LOCUS       DAYYAR010000001        57045 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000001 DAYYAR010000000
VERSION     DAYYAR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.001"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..1754
                     /locus_tag="ACHAT0_000001"
     CDS             123..1754
                     /locus_tag="ACHAT0_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8449983.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1816..2457)
                     /locus_tag="ACHAT0_000002"
     CDS             complement(1816..2457)
                     /locus_tag="ACHAT0_000002"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8449984.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2643..2719
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2739..3047
                     /gene="rplU"
                     /locus_tag="ACHAT0_000003"
     CDS             2739..3047
                     /gene="rplU"
                     /locus_tag="ACHAT0_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8449985.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3074..3418
                     /locus_tag="ACHAT0_000004"
     CDS             3074..3418
                     /locus_tag="ACHAT0_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8449986.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3431..3724
                     /gene="rpmA"
                     /locus_tag="ACHAT0_000005"
     CDS             3431..3724
                     /gene="rpmA"
                     /locus_tag="ACHAT0_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8449987.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4158..6965
                     /locus_tag="ACHAT0_000006"
     CDS             4158..6965
                     /locus_tag="ACHAT0_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8449988.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7156..7647
                     /locus_tag="ACHAT0_000007"
     CDS             7156..7647
                     /locus_tag="ACHAT0_000007"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8449989.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7750..8739
                     /locus_tag="ACHAT0_000008"
     CDS             7750..8739
                     /locus_tag="ACHAT0_000008"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8449990.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8772..9575
                     /locus_tag="ACHAT0_000009"
     CDS             8772..9575
                     /locus_tag="ACHAT0_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8449991.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9596..10510
                     /locus_tag="ACHAT0_000010"
     CDS             9596..10510
                     /locus_tag="ACHAT0_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8449992.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10593..10967
                     /locus_tag="ACHAT0_000011"
     CDS             10593..10967
                     /locus_tag="ACHAT0_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8449993.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11106..11351
                     /locus_tag="ACHAT0_000012"
     CDS             11106..11351
                     /locus_tag="ACHAT0_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8449994.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11523..12587
                     /locus_tag="ACHAT0_000013"
     CDS             11523..12587
                     /locus_tag="ACHAT0_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8449995.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12833..13258
                     /locus_tag="ACHAT0_000014"
     CDS             12833..13258
                     /locus_tag="ACHAT0_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8449996.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13251..13700
                     /gene="nusB"
                     /locus_tag="ACHAT0_000015"
     CDS             13251..13700
                     /gene="nusB"
                     /locus_tag="ACHAT0_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8449997.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13797..14651
                     /locus_tag="ACHAT0_000016"
     CDS             13797..14651
                     /locus_tag="ACHAT0_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8449998.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14652..15989
                     /gene="xseA"
                     /locus_tag="ACHAT0_000017"
     CDS             14652..15989
                     /gene="xseA"
                     /locus_tag="ACHAT0_000017"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8449999.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15994..16218
                     /locus_tag="ACHAT0_000018"
     CDS             15994..16218
                     /locus_tag="ACHAT0_000018"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8450000.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16220..17110
                     /locus_tag="ACHAT0_000019"
     CDS             16220..17110
                     /locus_tag="ACHAT0_000019"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8450001.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17119..17937
                     /locus_tag="ACHAT0_000020"
     CDS             17119..17937
                     /locus_tag="ACHAT0_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8450002.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            18044..18493
                     /locus_tag="ACHAT0_000021"
     CDS             18044..18493
                     /locus_tag="ACHAT0_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8450003.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18511..20193
                     /gene="recN"
                     /locus_tag="ACHAT0_000022"
     CDS             18511..20193
                     /gene="recN"
                     /locus_tag="ACHAT0_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8450004.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20248..20364)
                     /locus_tag="ACHAT0_000023"
     CDS             complement(20248..20364)
                     /locus_tag="ACHAT0_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8450005.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20437..21393)
                     /locus_tag="ACHAT0_000024"
     CDS             complement(20437..21393)
                     /locus_tag="ACHAT0_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8450006.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21564..21932
                     /locus_tag="ACHAT0_000025"
     CDS             21564..21932
                     /locus_tag="ACHAT0_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8450007.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22102..22533
                     /gene="mraZ"
                     /locus_tag="ACHAT0_000026"
     CDS             22102..22533
                     /gene="mraZ"
                     /locus_tag="ACHAT0_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8450008.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22554..23513
                     /gene="rsmH"
                     /locus_tag="ACHAT0_000027"
     CDS             22554..23513
                     /gene="rsmH"
                     /locus_tag="ACHAT0_000027"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8450009.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23518..23919
                     /gene="ftsL"
                     /locus_tag="ACHAT0_000028"
     CDS             23518..23919
                     /gene="ftsL"
                     /locus_tag="ACHAT0_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8450010.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23920..26112
                     /locus_tag="ACHAT0_000029"
     CDS             23920..26112
                     /locus_tag="ACHAT0_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8450011.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26138..27100
                     /gene="mraY"
                     /locus_tag="ACHAT0_000030"
     CDS             26138..27100
                     /gene="mraY"
                     /locus_tag="ACHAT0_000030"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8450012.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27114..28475
                     /gene="murD"
                     /locus_tag="ACHAT0_000031"
     CDS             27114..28475
                     /gene="murD"
                     /locus_tag="ACHAT0_000031"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8450013.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28501..29589
                     /gene="murG"
                     /locus_tag="ACHAT0_000032"
     CDS             28501..29589
                     /gene="murG"
                     /locus_tag="ACHAT0_000032"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8450014.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29604..30824
                     /locus_tag="ACHAT0_000033"
     CDS             29604..30824
                     /locus_tag="ACHAT0_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8450015.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30980..32305
                     /gene="ftsA"
                     /locus_tag="ACHAT0_000034"
     CDS             30980..32305
                     /gene="ftsA"
                     /locus_tag="ACHAT0_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8450016.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32328..33569
                     /gene="ftsZ"
                     /locus_tag="ACHAT0_000035"
     CDS             32328..33569
                     /gene="ftsZ"
                     /locus_tag="ACHAT0_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8450017.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33586..34263
                     /locus_tag="ACHAT0_000036"
     CDS             33586..34263
                     /locus_tag="ACHAT0_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8450018.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34277..34876
                     /locus_tag="ACHAT0_000037"
     CDS             34277..34876
                     /locus_tag="ACHAT0_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8450019.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34957..35178
                     /locus_tag="ACHAT0_000038"
     CDS             34957..35178
                     /locus_tag="ACHAT0_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8450020.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35227..36009
                     /locus_tag="ACHAT0_000039"
     CDS             35227..36009
                     /locus_tag="ACHAT0_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8450021.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            36030..36737
                     /locus_tag="ACHAT0_000040"
     CDS             36030..36737
                     /locus_tag="ACHAT0_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8450022.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36999..39797
                     /gene="ileS"
                     /locus_tag="ACHAT0_000041"
     CDS             36999..39797
                     /gene="ileS"
                     /locus_tag="ACHAT0_000041"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8450023.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39846..41372)
                     /gene="zwf"
                     /locus_tag="ACHAT0_000042"
     CDS             complement(39846..41372)
                     /gene="zwf"
                     /locus_tag="ACHAT0_000042"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8450024.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41387..42034)
                     /locus_tag="ACHAT0_000043"
     CDS             complement(41387..42034)
                     /locus_tag="ACHAT0_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8450025.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42218..42547)
                     /locus_tag="ACHAT0_000044"
     CDS             complement(42218..42547)
                     /locus_tag="ACHAT0_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8450026.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42724..43452
                     /gene="sfsA"
                     /locus_tag="ACHAT0_000045"
     CDS             42724..43452
                     /gene="sfsA"
                     /locus_tag="ACHAT0_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8450027.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43468..44481
                     /locus_tag="ACHAT0_000046"
     CDS             43468..44481
                     /locus_tag="ACHAT0_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8450028.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44481..45758
                     /locus_tag="ACHAT0_000047"
     CDS             44481..45758
                     /locus_tag="ACHAT0_000047"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8450029.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45821..48523
                     /locus_tag="ACHAT0_000048"
     CDS             45821..48523
                     /locus_tag="ACHAT0_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8450030.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48675..48992
                     /locus_tag="ACHAT0_000049"
     CDS             48675..48992
                     /locus_tag="ACHAT0_000049"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8450031.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            49022..49342
                     /locus_tag="ACHAT0_000050"
     CDS             49022..49342
                     /locus_tag="ACHAT0_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8450032.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49450..50910
                     /locus_tag="ACHAT0_000051"
     CDS             49450..50910
                     /locus_tag="ACHAT0_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8450033.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50978..51199
                     /locus_tag="ACHAT0_000052"
     CDS             50978..51199
                     /locus_tag="ACHAT0_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450034.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51230..51412
                     /locus_tag="ACHAT0_000053"
     CDS             51230..51412
                     /locus_tag="ACHAT0_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8450035.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51412..51825
                     /locus_tag="ACHAT0_000054"
     CDS             51412..51825
                     /locus_tag="ACHAT0_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8450036.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51948..53129
                     /locus_tag="ACHAT0_000055"
     CDS             51948..53129
                     /locus_tag="ACHAT0_000055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8450037.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53200..53364)
                     /locus_tag="ACHAT0_000056"
     CDS             complement(53200..53364)
                     /locus_tag="ACHAT0_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450038.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53518..53591
                     /locus_tag="ACHAT0_000057"
     tRNA            53518..53591
                     /locus_tag="ACHAT0_000057"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53552..53554,aa:Arg,seq:cct)
     gene            complement(53660..54799)
                     /locus_tag="ACHAT0_000058"
     CDS             complement(53660..54799)
                     /locus_tag="ACHAT0_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8450039.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            54971..55105
                     /locus_tag="ACHAT0_000059"
     CDS             54971..55105
                     /locus_tag="ACHAT0_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450040.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(55098..55733)
                     /locus_tag="ACHAT0_000060"
     CDS             complement(55098..55733)
                     /locus_tag="ACHAT0_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450041.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(55846..56481)
                     /locus_tag="ACHAT0_000061"
     CDS             complement(55846..56481)
                     /locus_tag="ACHAT0_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8450042.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(56515..56976)
                     /locus_tag="ACHAT0_000062"
     CDS             complement(56515..56976)
                     /locus_tag="ACHAT0_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8450043.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT        18692 a         9663 c        12095 g        16595 t
ORIGIN      
        1 atatagaaaa aataagaatt tatctaacag gaaactttgt gaaaaatatt actttatctc
       61 atgaaatatg atattctaat gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat
      121 aggtgttcaa aaattatgtt tcctcactaa aaaaagaatt ttcaggttat gatctttcaa
      181 gatttcagaa agatcttttg gctgggatca cagtagctgc tgtagcattg cctttagcct
      241 tggcttttgg ggtatcaagc ggagctgatg cggccgcagg tttgattacg gctatcattg
      301 ccggtatcgt tattggcggt ttatcaggcg gtttttatca aatatcaggt ccgacaggtg
      361 cgatggcagc tatcttgatg tctattgcgg caacaaaggg aatgcaaggt gtattgcttg
      421 ctactttttt agcgggtcta tttttacttg cagcaggact tttccgatta ggaacactga
      481 cttcttttat cccagcaccc gtaattacgg ggtttacttc aggtattgct atcattattg
      541 cattaggtca gatcgataat ttatttggtg ttcactcgga aggtacaaac gttattgaaa
      601 aactaagtag ttatcaaacg ataggatttc cgataaacac agcctctttg atcatgggag
      661 ggctagtcat tttagggatg tttatctatc caaaaaaatg ggcgaaacga atgcctagtt
      721 cgctgctggc tatcattttg gcaacagcag ttatgctatt gttccattta ccagtagcaa
      781 ctgtgggtaa gattccacaa acattgatca gtagtaatcg tttgaatatg ggagatttta
      841 gcttttctgc cttgcaacaa gtggcagttc cagctattag cattgcttta ttaggaatga
      901 ttgaaagctt gctatgtggt gcttcggctg gccgaatggc aaataagccc ttagatagca
      961 atcaagaatt aatggcgcaa ggaattggaa atctgttgct tcctttcttt ggcgggattc
     1021 ctgctactgc ggccatcgcc cgtacaagcg tggccatcaa atctggtgca caaactagat
     1081 tagctggtat gatacatgca gttgtcttac tgctctccat gcttgttttt gcgccagtga
     1141 tgtcacagat tccaatgcca gcacttgcag gtgttttgat tgtgacggct tggcgtatga
     1201 atgaatggga aacgatcaag gagctattta ctaataaata ttggtcagca gttctattgt
     1261 ttatccttac gatgatttgt acagttatct ttgatttaag tattgctatc gtgatcgggg
     1321 tgatcggcgg ctgtgtattt tttgttgtca aaagcgctgc tatcacgatt tcattagaaa
     1381 cgatcgattg gcaaagaatg gggctgccag aaacggaaaa gctgaataac tggacagtag
     1441 tgtatattag tggtccactg ttttttatgt cttcagaaaa actcaaaaat aaattggaag
     1501 aactaaatca tcacgatggt gtcattttct ccatgcgagg cgtaccaagt attgatttga
     1561 cggcacgtgc agtttttgat gaatttcaag aaaaagcaga gcaaagagga caaacgatta
     1621 tctatacttc cttgcagcca gaagtagaaa aacaactttc tcacttgtgg gaacatacat
     1681 tcacgcatca acatcaaaca gttgctcatg cgttgacagc tttacatgcg aaatatactg
     1741 caattagcca ttaaaacaat gaaaaagaac agacaagact gtaaactgaa gattttatgg
     1801 tcttgtctgt tctttttaat attgctgtaa aagtgaagct agtacttgcc agtcttgatg
     1861 gataacaggt acaagggagc gattgtagaa aatagctgct ggatgaaaaa gtggaagaat
     1921 tcgatagttt ttttctgacc attcgtaggc atccttttgt tctgttaatt gtaagacagg
     1981 ccctgtatag agttgaccat gatttttggt aaccgtatat tctttgccaa gtaaacgttg
     2041 aagaccaatg tttccaagtg tcgcaattaa ttttggctgg accttcatca gttcgtaatc
     2101 caatattggt gcatgcgcaa atacttcgga acgagtaggt gtccgatttg gataaacagt
     2161 ttctgactgt tgtgtccgtt tattgatccg atgagtcacg cgatacgggc ggctccttac
     2221 agcactggtg atatacacct cttctcgtgt taagccagtt gaagaaagtg cctgcatcaa
     2281 ttcttttcca gcttgaccgc taaaaggaac atgcgtatca atctctgttt tccctggggc
     2341 ttctcccacg agcatcagtt taggatgaag aggtccttgc ccagcgataa atccttctac
     2401 aggacggccg ttcattctta aagaagcttc ttgtatcagt tcttttgggt attccattta
     2461 tgctcctcct ttttctttta ttataagaat aaaagaaaca caatgcgact attttatttg
     2521 gtagaactgt gaggatagaa aagcagaagt attgcttgaa tttttgtttt actttttgat
     2581 tttaaattta gttgactaac ggttgctgtt ctggtaagct agtactgttg taattgtgga
     2641 ctccacagct acaaccgctc agaacaagta ataagcacag catatgctgt cacttcggat
     2701 ggcgagtcta agtatatcag aaggaggtgc tttatagcat gtacgcaatt attaaaactg
     2761 gtggtaaaca agtaaaagtt gaagtgggac aagcaatcta tgttgaaaaa cttgacgttg
     2821 aagcaggaga aaaagttgtt tttgacgaag ttatcttagt aggcggagaa tctacaaaag
     2881 taggcgctcc aactgttgca ggtgcaactg ttgaaggtac tgttgaaaaa cacggcaaac
     2941 aaaagaaagt cgttactttc aaatacaaac ctaaaaaaca ttcacaccgt aaacaaggtc
     3001 atcgtcaacc atatacaaaa gtggttatcg acgcgatcaa cgcttaatag cgtttgagaa
     3061 agaaggccta tatatgatca aaggaacatt taagcgaaat gacgcaggtc agatcgtttc
     3121 attcactttg accgggcatg ctgatgctgg tccttatgga agtgatatcg tttgtgcggg
     3181 tgtttctgcg ttagcgatca gcactgtcaa cgggattgcc tcattagccg gttttgagcc
     3241 gattgttgaa atgaatgaag aagaaggcgg ttatctttat accgaagtca cttcaggaat
     3301 gacacaagaa caaaacaata ttgctgagat tctattagaa aatcttttat taggattaca
     3361 atcgatagaa gctgaaaact cagaatatat ccaaattaaa acaattaatg ataaatagga
     3421 ggtgcagacc atgttgttat ctatgaatct acaattattt gcccacaaaa aaggtggcgg
     3481 ttctacatct aacggccgtg actcagaatc aaaacgtcta ggtgctaaac gtgctgatgg
     3541 acaaacagtt acaggtggat caattcttta ccgtcaacgc ggtacaaaaa tctatccagg
     3601 tgtaaacgtg ggtatcggtg gagacgacac tttatttgca aaagttgatg gcgtagtacg
     3661 tttcgaacgt aaaggccgta acaaaaaaca agtgtctgtt tatccagtag ctcaagaagc
     3721 ttaattgaat cttatgatta aagaggttgt gacaagactt ttgtcacagc cttttttgtt
     3781 tataaaaata aaagttgtag tggaggctac gaagtttact ttgtcttttt acacttaata
     3841 tttttttgcc aatttggaat caggatgttt tgataactat cgttttcaat tgatggaagc
     3901 tatattgttt gctatcagtg aaataattat atggtaaaca gtgttctttt tcttgtttcc
     3961 attataaaaa tcgtatcggg atttttcgtt cttttaaatt gaacatttct atctatccgc
     4021 gtgttatcaa caaaacaaaa tcaggtaaat agttgacgaa acagaaataa atacatagta
     4081 ttatgttttg aagcctaagt acaatagtct atagaaaagg agcgatacca ttggcaaaac
     4141 gcattgaccg aatttatatg tatgtcaaag aaaaaaccgc tcatttaaca aaggcagaat
     4201 atgatcaagg agtaacaaca caggaaattg ctgaagtgtt agggattcag cgaacaaata
     4261 gcagtaaaga tctgaatcag ctagtgcgcg aaggaaaact gataaagaca gacggacgac
     4321 cagtacgtta catatatcaa acactcgtta cgcacgtaaa gccagttaca aaacatgtag
     4381 tcagttacaa agaaacgccg gtctctgaag aaaaaaatac actccaaata gatactaagg
     4441 atatctttgc aaaaatcatt ggagctaacg gcagcatgaa aaattcagtg gaacaggcaa
     4501 aagctgcgat tttgtatccg ccaagaggat taaattgttt gattacagga cctaccggat
     4561 caggaaagac ttattttgca cacgcgatgt atcattttgc aaaagcgcat catgtaatag
     4621 atgaggaaac ggaactgatt gttttcaatt gtgctgatta tgccaataac cctgagcttt
     4681 tgatgagtca tttatttggg tacgcaaaag gtgcttttac aggcgcagat gaagaaaaaa
     4741 tggggattat cgatcaagcg gatggcggta tgctgttttt ggatgagatt caccggttgc
     4801 cgcctgaagg tcaagaaatg atcttttact ttatggatca tggaacttac agtcgtcttg
     4861 gggaaacaac gaaatcccat gaagcaaatg tacggatcgt aggtgcgacc acagaagacc
     4921 caggttcttc tctattagaa acttttgttc ggcgtatccc aatcaatatc aaacttccgg
     4981 cttttgaaaa acgtcctgca aatgagaaag tcgatttagt aaaaatcatg attgcccacg
     5041 aagcaaaccg cacgcagcga aagatttctt tgacagaaga tgtagcaaaa gcattgatcg
     5101 gcagtgtgac ctatggaaat ataggacaat tgaagtcaaa tatccagtta gtatgtgcac
     5161 gaggatttct aaatcatatg caatcacccg aaatctcgat cacaatcgat gatttgacag
     5221 aagggattcg tagcggattg attcaattag caagcaaccg cagtgcaatg gcagagcttt
     5281 ctaaaatatt ggagccaaag ataactgtag caccaaatga tacgatcatg aaaatccaat
     5341 cggactccta tgagttacct tataatctat atgatatcat cggagataaa gcagctttac
     5401 tgaagtcgga tggtttggat caagaagcaa tcaatcattt catttctaca gatatcaaca
     5461 tccatctgaa gtctttctat aaagaccatg gtttttcttt taatgcagac agcaaattag
     5521 cagaatttgt agatccgaaa atcatagaag tgaccaatca gatttatgta atggtcaaaa
     5581 gatctttacc atatgagttt cagcaaaact ttatttatgc tatgagcttg catatcagtt
     5641 cttttttgaa aaaaatcaac ttaggagaag aacggcatac gaatgataac atccgtgaga
     5701 tggcttttga ttttccagat gaatatgctg ttgcgaaaga agtgcgtagt tatatcgaaa
     5761 cgtattttca agtggagatt ccagaaagtg aagactatta cttgaccgtt ctactcgtct
     5821 cgttacgggc aaatcaagca agcggcagaa tcggtgtagt cgttgcagct cacggaaaca
     5881 gcacagcaag cagtatggtc caagttgtcc aacagttgtt agatgcagag aacgtacgag
     5941 cagtagacat gcctttggat atggatccga aaacagcatt ggaccgaatt gaacgcaatg
     6001 tacaagaagt agatgaagga agcggggtca tactcttagt agatatgggg tcccttgcgt
     6061 cgtttaattc gcaaattcaa agagacactg gaattccagt acgtactgtt gatatggtaa
     6121 caacttctct agttttggaa actgtacgaa aggtctcagt attagggaca gatctagata
     6181 tgttgcatga atcattgaaa aattttcgag gttacgcaga aatatcttct gtagaatctg
     6241 ttcaggaagc aaaacctatt cataagaaaa aagctgtttt ggctatctgc gcatctggcg
     6301 aaggtacagc acaaagaatc aaagaattga tcgaacgagc ggtaaacaag cgtcaagaaa
     6361 cagaattaga agttctagct ttgtcgattg tggaagtgaa agaagaactg ccagttatcc
     6421 aaaaagaata tcagatcata gctactactg ggattactga tttgaatatc caagccccat
     6481 ttattccttt agaacgtttt attgatcaaa atatcgaagt cattttggat cagttgctga
     6541 tggaatcaga attggaggaa actgaattca tctccctaga tgaagagtca gctaaaaata
     6601 cgtgtgttga gtttatctct gataacttca tctttattaa tggtagtaag ttaatcgatc
     6661 caatgtggca attttctaca caaatctctc agacgacggg aatcggcgat gaagaatatg
     6721 gattcaaaat caacctagtg atgcacacag caggaatgat cgaacgtatt attcggaatg
     6781 aaccattgac tgtggaagaa aacgaattga caaatacaac gaatgatcct ctttatagcc
     6841 aattggcagc ctcagttgtt ctgttggaag atcagataaa agtaaaagtg ccgatagaag
     6901 aaatgtatta tctgctacgt ctagtccata atcagttaga taaaaaagaa tacactgtgc
     6961 catgaataca caaaaacgat acacttttat agtgtatcgt ttttgtgtat tggttaagaa
     7021 tcctttaaat tcaattagtg tatcgtaaaa aagttggcac gctttttgct tatagtagtg
     7081 tgtgctagga ggtggttgtt cacacagaaa tgataggggt ttctgtgaag tagtgaggaa
     7141 gaaggagttt tgcagatgga tattcgattg gcacgtatag atgatcgttt aatccacggg
     7201 caagtcgcaa cagtttggtc gaagcaaaca ggtgttgaac gtatcattgt gatcagcgat
     7261 gtagtcgctc aagataaatt gagaaagttt ctactaaaag aagctgcacc gccggggatc
     7321 acagcaaatg taataaccgt gcagaaaatg attcaaatct atcatagtgg attattatca
     7381 cgagcaaaag tgatgctttt attcaccaat ccccaagatg ttgaagcggt tgtccgctgc
     7441 ggtgtacagt tgaagtcgtt gaatatcggc ggtatgagtt ttactgaagg caaatcaatg
     7501 attaccaatt ttgtttcagt aaatcaacaa gatattgatt cctttcgtta cttgaacagt
     7561 caagaagtgg agttagagat tcgaaaagta cctgctgaca ggaaaatcct tttgatggaa
     7621 ctactagcga aaatcaaaaa ggtgtagcaa gaatggttgt tggaaaaggt caaataattc
     7681 tgttgtaatg gttagcaatt attaatattt ttgaatttgt tacaacaaaa gtaaatagga
     7741 ggtataaata tggtagggat tatcctagca agtcatggac aatttgctga aggaatctta
     7801 caatccggtt caatgatctt tggcgaacaa gaaaacgtca aagccgtgat tttgaaacca
     7861 agtgaaggtc cagatgactt gagagccaaa ttggaagaag cggttgcttc ttttgataat
     7921 caagacgaag tgttattctt agttgattta tggggcggaa caccgttcaa tcaatcaaac
     7981 acattattcg aagagcacaa agataaatgg gcaatcgtca gcggtttgaa tttaccaatg
     8041 ttgattgaag cctacgcatc acgcttttca atggaatctg ctcatgaaat tgcagcacac
     8101 atcattgaaa cagcaaaaga cggtgtaaaa gtaaaaccgg aagaattaga accggcagaa
     8161 gcaccgaaag cagcagttga agacgcacaa cctaaaggag cacttccaga aggaactgtg
     8221 gtcggagacg gaaaaatcaa atatgtatta gcacgtgttg actcacgtct gttacatgga
     8281 caagttgcga cagcttggac aaaagcagtg caaccaaacc gtattatcgt tgtttcagat
     8341 gcagtatcaa aggatgatct gcgtaagaga ctaatcgaac aagctgctcc tccaggtgta
     8401 aaagcaaatg ttattcctat tagcaaaatg atcgaagtag caaaagaccc tcgttttgga
     8461 aataccaaag cattgctttt atttgaaaat cctgaagacg tattgacagc tgttgaaggt
     8521 ggcgtagaca tcaaagaatt aaatgttggg tcaatggcgc attcggtcgg taaagtcgtt
     8581 gtaagtaaag tattgtctat gggacaagaa gacgttgaag cgtttgagaa gcttgaacaa
     8641 aaaggtgtga aattcgatgt acgtaaagta cctaatgatt cacgcgataa catggatgat
     8701 attttgaaaa aagcaaaagc tgaattagca aaagcgtaat aaataatatt aaaatttata
     8761 ggaggcttat catgtctatt atttcaataa ttttagtcgt attagttgcc tttctagctg
     8821 gtatggaagg tattcttgat gaatttcaat ttcatcaacc actagtggca tgtacattga
     8881 tcggtttagt taccggtaac ctcgaagctg gtatcgtact tggcggtacg cttcagatga
     8941 tcgctttagg ttgggcaaat atcggtgccg ctgtagcacc agatgctgca ttagcatcag
     9001 tagcatcagc aattatttta gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga
     9061 tcgcgattgc cgttcctttg gctgttgcag ggttgttctt aacaatgatc gtacgtacaa
     9121 tcgctgttcc aatcgttcat ttgatggatg cggctgctga agaaggaaac atccgtaagg
     9181 ttgaaatgtg gcacatcatt gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag
     9241 cagcgttgct atttatccca gcacaaactg tgcaatcatt ccttgaatca atgccagcat
     9301 ggttaacaga tggtatggct atcggtgggg gaatggtcgt agctgtaggt tacgctttgg
     9361 taatcaacat gatggctaca aaagaagtat ggccattctt cgttattggt ttcgttgtag
     9421 ctgcaatttc tcaattaaca ttgattgctc taggtgcttt aggtgttgca ttagctctta
     9481 tttacttgaa cctttctaaa atgggtggtt catcaaataa tggaggcgga agcaattctg
     9541 gtgacccttt aggcgacatt ttgaatgact attaatcttg aaggaggaaa ataaaatggc
     9601 agaagaaaaa atcaaattat caaaaaaaga tcgtttagct gttgcgtggc gctctacctt
     9661 cattcaaggt tcatggaact acgaacgtat gcaaaacggt ggctgggcat tttctatgat
     9721 tccagcaatc aaaaaattat acaaaacaaa agaagaacgt tctgcagcat taaaacgtca
     9781 cttggaattc tttaatacac acccatacat tgcttctcca attttagggg ttacattggc
     9841 tctggaagaa gaacgtgcga atggcgcgcc tgttgatgat gtagcgattc aaggggttaa
     9901 agtaggtatg atgggtcctt tagctggtgt tggtgaccca gtgttctggt ttacagtacg
     9961 tccaatgcta ggtgcactag gtgcttcatt agctatggga ggaaatatcc ttggaccaat
    10021 catcttcttc ttaggttgga acattattcg ttgggcattc atgtggtata cacaagaatt
    10081 tggctacaaa gctggttcta aaattactga tgacctttct ggcggattgt tacaagacgt
    10141 aacaaaaggt gcttcaattt tagggatgtt cgtattagct gcccttgtac aaagatgggt
    10201 atcaatcaaa ttcttaccag ttgtatcaac tgtaaaatta gataaaggtg cttacatcga
    10261 atgggataaa ttgccttcag gtggtgaagg aatcaagagt gcatttgaac aagttaataa
    10321 tggcttagcc ctttctcctg aaaaggtaac aacattacaa gacaacttag accaattgat
    10381 tcctggttta gctgcattag gattaacatt gttatgtatg tggttgctta agaaaaaggt
    10441 ttctccaatc gttatcatcc ttggtttatt cgtagtcggt gttgttggac acgtcatcgg
    10501 acttttataa tccataaaaa tatagctcgg gcgttgtccc gagctttttt tatatagtat
    10561 actgttcata aacaaatatc tggagggaga aaatggttca atcaatcaat acaaaagtag
    10621 acttggtaat tgatgctact tcgtttacgg gtctgacaga ttacgggcaa atcatgatag
    10681 gtaataatgg ttttgagttt taccatgcgc gagatagacg caaatatatc caaattccat
    10741 gggaagaagt ggattatgta ctagcatcag tcatgctaaa gggaaaatgg attcctcgat
    10801 atgccattca aacgaaaaaa aatggtacat acacattttc ttccaaagaa cctaaaaaag
    10861 tgcttcgtgc gatcagagaa tatgtagatg aagatagaat ggtacaatca ttgagtttct
    10921 ttgatgtagt gaaacgtggt attcgtagta tgtttaaacg caagtaaaaa ataaagaaaa
    10981 cttctaagag gaggttttct ttattttttt actttgcaga agaaaatatg aaatagcaaa
    11041 accaattatt tgtccccttt aaatcccata tgctaaaatt gattagcaaa gaaggaggtt
    11101 atattatgag ttggttatgg acattgattg ttggagccgt gatcggagca attgcaggtg
    11161 cgatcaccag taaaggaaat tcgatgggct gtgtaacgaa tatcattgct ggactagtcg
    11221 gttcaagtat tggacaagcg attttcggcg aatggggacc aaaagctgca ggtatggcac
    11281 ttattccatc tattttaggc gcagtgattt tggtagcagt tgtctcattt ttctttggaa
    11341 aacgatcata agtttttaca aaatagtaaa ctaaaaggag ttgcagcgag actgtgactc
    11401 ctttttggct ttatttattt aaaataaaaa gaagttttcc ggaaattttt atttaactgt
    11461 cgtgtacgaa gaaaagtttg ctataatgga aaaaagcgtg caaagaaaga gggagtttca
    11521 ttatgatgct acgtgtagaa aaattgcgca aaaaaatgca agaagaaaat ttggattcat
    11581 ttttggttac cagtccgtac aatcttcgtt acttaacgaa ctttacaggt acgacaggat
    11641 tagctgttat tactttggaa aaagcatttt ttattactga ttttcgttat accgagcagg
    11701 cggcagcgca ggctcaaggg tttgaaatta ttaaaaatgt cggacctatt tttgatgagg
    11761 tagcaaactt agtacgcaaa gaagaactgc actcattagc ttttgaggaa acaacagtct
    11821 catttttaga atattcggtt ttagaagaaa tcatcgatgc ggaattagta cctgtttcac
    11881 agatgatcga agaactcaga gaagtgaaag atgaagaaga aatcgctatt atcgaaaaag
    11941 catgtcatat tgcggatatg gcatatgatc acatcctgaa aatgatccaa ccgggcatga
    12001 ctgagattga agtcgcaaat caactagact tttatatgcg ttctcttggc gcttcaggtg
    12061 tttcttttga tacgatcgtt gctagcggat tgcgttcggc tatgccgcat ggtgtagcaa
    12121 gcaaaaaaat catcgaacaa ggtgacttga tcacattgga ctttggttgc tattacgaag
    12181 ggtacgtttc cgatatgaca cgtacattcg ctattggtga tcctggggaa aaattaaaag
    12241 aaatttatca aatagtctta gatgctcaat tagcagttat tgatgctgca aaaccaggaa
    12301 tgactggtgt ccaattagat gcagttgcta gagattatat ttcaaaacat ggttacgggg
    12361 aagcatttgg acattctaca ggacatggga tcggtttaga gatccacgaa ggaccaaacg
    12421 tttcttctcg tgcagaaaag caatttgtac caggaaatgt gatcactgat gaaccaggga
    12481 tctacttacc aggaatcggt ggagtacgta ttgaggatga cttgttgatc acagaagatg
    12541 gaaatcgtgt gttgactcgt tcaccaaaag aattgatcat cttatagttt catcaataga
    12601 agaagtaagc gtaaaaatca gtgttcacag actttgttat agaaaatcac tcgatttaat
    12661 gatacactat agaagaactt ttaagctgta agagataata aagttgggtg ttttctccaa
    12721 ttcatagcag tttgcagctg atcggcggca gttttcttac cacgtgatca ctatgccgca
    12781 tcatagagaa ttcggatgta tctcgtgctt aggcaaaggg aggaataaat aaatggcaga
    12841 tgaaaaaaat ttgattttaa acgcaaacca agatttagga gagatcgtga tcgctcctga
    12901 agtcatcgaa gtaattatcg ggattgcagc ttctaaagta gaaggcgtat acggaatgcg
    12961 cggaactttt gccagcaatg tcactgaatt attaggacgt gcagctcacg gaaaaggtgt
    13021 ttacttagca aacgaggaag atggcttgaa agtagatctt tactgttatc tagaatatgg
    13081 tgtttcagtt cctaaagtag caatggaaat gcaggaacgg gtcagccagc aagtcctttt
    13141 catgacggat atcgatttat ctgaagtcaa tatccatgta gtagctgttg ttcctgaaaa
    13201 attagaacaa ccaacaatcg aagaactttt tgaagacgaa gaggaagaga atgaataaag
    13261 aattatcaag acacgagatt cgggaaatgg cattgcaggc tttatttcca ttagacttca
    13321 atgcagacct aacaaaagaa gatgcgattt tcaacgcaat cgaattagac caccgcgaca
    13381 tgatcaacga agatgaatca gagtttgttc ctgtttatct tgatacgcta gttggcggtg
    13441 tctgtgcaaa aaaagatgaa ttggataaag tgattgaaaa acatttaaaa aacaattggc
    13501 acatcaatcg gatctcaaaa atggatttgg tgatcttgcg tatcgcgatt tttgaaatga
    13561 cttatgtgac agatgtacca gcggctgttg ctttaaatga agcaatcgag ttggcaaaaa
    13621 catttagtga tgaccgttca cgtaaattcg tcaacggtgt tttatccaat gtattgaaag
    13681 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
    13741 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
    13801 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
    13861 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
    13921 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
    13981 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
    14041 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
    14101 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
    14161 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
    14221 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
    14281 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
    14341 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
    14401 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
    14461 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
    14521 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
    14581 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
    14641 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
    14701 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
    14761 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
    14821 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
    14881 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
    14941 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
    15001 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
    15061 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
    15121 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
    15181 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
    15241 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
    15301 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
    15361 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
    15421 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
    15481 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
    15541 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
    15601 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
    15661 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
    15721 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
    15781 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
    15841 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
    15901 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
    15961 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
    16021 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
    16081 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
    16141 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
    16201 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
    16261 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
    16321 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
    16381 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
    16441 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
    16501 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
    16561 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
    16621 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
    16681 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
    16741 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
    16801 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
    16861 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
    16921 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
    16981 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
    17041 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
    17101 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
    17161 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
    17221 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
    17281 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
    17341 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
    17401 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
    17461 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
    17521 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
    17581 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
    17641 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
    17701 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
    17761 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
    17821 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
    17881 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
    17941 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
    18001 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
    18061 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
    18121 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
    18181 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
    18241 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
    18301 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
    18361 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
    18421 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
    18481 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
    18541 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
    18601 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
    18661 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
    18721 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
    18781 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
    18841 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
    18901 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
    18961 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
    19021 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
    19081 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
    19141 gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
    19201 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
    19261 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
    19321 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
    19381 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
    19441 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
    19501 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
    19561 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
    19621 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
    19681 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
    19741 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
    19801 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
    19861 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
    19921 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
    19981 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
    20041 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
    20101 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
    20161 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
    20221 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
    20281 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
    20341 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
    20401 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
    20461 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
    20521 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
    20581 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
    20641 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
    20701 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
    20761 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
    20821 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
    20881 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
    20941 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
    21001 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
    21061 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
    21121 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
    21181 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
    21241 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
    21301 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
    21361 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
    21421 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
    21481 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
    21541 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
    21601 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
    21661 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
    21721 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
    21781 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
    21841 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
    21901 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
    21961 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
    22021 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
    22081 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
    22141 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
    22201 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
    22261 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
    22321 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
    22381 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
    22441 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
    22501 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
    22561 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
    22621 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
    22681 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
    22741 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
    22801 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
    22861 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
    22921 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
    22981 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
    23041 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
    23101 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
    23161 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
    23221 ccgaagaggc ctctttagaa caggcaatcc ggttattgaa agtcaatgga agaatcagcg
    23281 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
    23341 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
    23401 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
    23461 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
    23521 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
    23581 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
    23641 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    23701 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    23761 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    23821 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    23881 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    23941 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    24001 attttatttg ctacgagtat tggattgttc tttttatttg ccgtccgttt ctcgtatatc
    24061 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    24121 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    24181 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    24241 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    24301 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    24361 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    24421 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    24481 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    24541 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    24601 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    24661 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    24721 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    24781 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    24841 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    24901 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    24961 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    25021 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    25081 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    25141 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    25201 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    25261 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    25321 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    25381 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    25441 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    25501 gtacctggat acaatgtatc ggcaaaaaac ggaacagccc aaatcgcttc tgatacaggg
    25561 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    25621 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    25681 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    25741 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    25801 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    25861 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    25921 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    25981 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    26041 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    26101 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    26161 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    26221 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    26281 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    26341 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    26401 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    26461 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    26521 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    26581 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    26641 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    26701 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    26761 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    26821 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    26881 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    26941 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    27001 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    27061 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    27121 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    27181 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    27241 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    27301 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    27361 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    27421 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    27481 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    27541 cacgtttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    27601 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    27661 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    27721 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    27781 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    27841 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    27901 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    27961 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    28021 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    28081 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    28141 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    28201 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    28261 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    28321 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    28381 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    28441 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    28501 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    28561 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    28621 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    28681 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    28741 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    28801 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    28861 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    28921 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    28981 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    29041 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    29101 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    29161 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    29221 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    29281 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    29341 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    29401 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    29461 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    29521 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    29581 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    29641 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    29701 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    29761 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    29821 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    29881 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    29941 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    30001 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    30061 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    30121 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    30181 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    30241 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    30301 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    30361 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    30421 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    30481 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    30541 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    30601 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    30661 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    30721 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    30781 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    30841 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    30901 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    30961 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    31021 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    31081 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    31141 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    31201 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    31261 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    31321 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    31381 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    31441 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    31501 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    31561 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    31621 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    31681 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    31741 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    31801 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    31861 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    31921 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    31981 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    32041 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    32101 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    32161 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    32221 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    32281 tttctttaca aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa
    32341 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    32401 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    32461 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    32521 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    32581 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    32641 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    32701 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    32761 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    32821 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    32881 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    32941 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    33001 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    33061 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    33121 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    33181 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    33241 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    33301 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    33361 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    33421 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    33481 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    33541 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    33601 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    33661 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    33721 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    33781 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    33841 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    33901 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    33961 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    34021 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    34081 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    34141 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    34201 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    34261 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    34321 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    34381 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    34441 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    34501 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    34561 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    34621 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    34681 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    34741 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    34801 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    34861 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    34921 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    34981 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    35041 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    35101 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    35161 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    35221 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    35281 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    35341 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    35401 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    35461 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    35521 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    35581 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    35641 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    35701 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    35761 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    35821 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    35881 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    35941 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    36001 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    36061 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    36121 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    36181 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    36241 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    36301 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    36361 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    36421 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    36481 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    36541 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    36601 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    36661 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    36721 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    36781 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    36841 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    36901 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    36961 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    37021 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    37081 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    37141 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    37201 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    37261 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    37321 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    37381 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    37441 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    37501 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    37561 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    37621 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    37681 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    37741 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    37801 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    37861 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    37921 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    37981 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    38041 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    38101 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    38161 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    38221 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    38281 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    38341 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    38401 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    38461 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    38521 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    38581 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    38641 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    38701 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    38761 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    38821 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    38881 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    38941 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    39001 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    39061 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    39121 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    39181 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    39241 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    39301 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    39361 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    39421 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    39481 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    39541 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    39601 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    39661 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    39721 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    39781 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    39841 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    39901 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    39961 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    40021 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    40081 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    40141 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    40201 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    40261 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    40321 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    40381 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    40441 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    40501 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    40561 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    40621 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    40681 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    40741 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    40801 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    40861 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    40921 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    40981 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    41041 ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    41101 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    41161 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    41221 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    41281 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    41341 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    41401 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    41461 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    41521 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    41581 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    41641 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    41701 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    41761 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    41821 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    41881 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    41941 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    42001 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    42061 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    42121 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    42181 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    42241 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    42301 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    42361 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    42421 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    42481 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    42541 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    42601 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    42661 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    42721 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    42781 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    42841 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    42901 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    42961 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    43021 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    43081 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    43141 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    43201 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    43261 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    43321 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    43381 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    43441 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    43501 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    43561 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    43621 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    43681 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    43741 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    43801 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    43861 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    43921 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    43981 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    44041 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    44101 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    44161 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    44221 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    44281 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    44341 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    44401 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    44461 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    44521 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    44581 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    44641 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    44701 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    44761 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    44821 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    44881 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    44941 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    45001 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    45061 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    45121 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    45181 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    45241 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    45301 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    45361 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    45421 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    45481 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    45541 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    45601 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    45661 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    45721 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    45781 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    45841 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    45901 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    45961 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    46021 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    46081 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    46141 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    46201 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    46261 ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    46321 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    46381 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    46441 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    46501 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    46561 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    46621 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    46681 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    46741 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    46801 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    46861 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    46921 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    46981 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    47041 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    47101 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    47161 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    47221 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    47281 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    47341 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    47401 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    47461 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    47521 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    47581 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    47641 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    47701 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    47761 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    47821 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    47881 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    47941 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    48001 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    48061 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    48121 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    48181 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    48241 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    48301 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    48361 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    48421 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    48481 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    48541 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    48601 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    48661 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    48721 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    48781 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    48841 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    48901 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    48961 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    49021 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    49081 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    49141 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    49201 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    49261 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    49321 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggaagaag aaaaattttt
    49381 gaacggattt tttattttgt tataaggaga actctcttta tataaaaaac ttataggagg
    49441 aagtaaaaaa tgaacggact atcaagttgg atggagaaat acatcctgcc ggttgccgca
    49501 aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg cttttatcgg catactgccc
    49561 gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa
    49621 cagtttaaca gtacctatgc tgacagttta gcagcagaca aaggaatctt tgccgtattg
    49681 aatgtcatca tcggaatcaa tggttttgtc tggaacggta cactagcaat cgccggattg
    49741 atctttgcct tttcttgggg gtataatctt gcccgagctt atggagtaaa tgatctagct
    49801 ggggggatcg ttggtttagc taccttgatt caaggtattt cctttggtcc agcattatca
    49861 gcagcattag atgtgaatgt tccttcaaat gtcgcagatg caatcaatag ttctgagatt
    49921 ggtgcaacga ttgcagatgg cacactgtca gttggattat ggggttggct aaaactagat
    49981 catttgaatg gaaacgctta ttttacagtt atgatcatgg gagctatttc agtcattatt
    50041 ttttgtaaat tgatgcttgc caacattacg atcaaaatgc ctgattcagt accaccagca
    50101 gtatctaaag catttgcagc cattttacca gcgacgatcg ctttatacgt cattgctatt
    50161 atcaattata tcgtaggtcg tgtagctgat gggcaactga tcattgattt ggtgcaaaaa
    50221 tatattgcag aaccattcct agggttatct caagggttgg gagcagtatt gatcgtcaca
    50281 gtatttgttc aagtattctg gttctttggt attcatggat caaatgtatt ggcaccagtg
    50341 ctagagggaa tctggggaca agcacaacta atcaatattg atattttcca aaaaggttat
    50401 aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg aagatggaaa agcttatatg
    50461 tgggtccgcg gatcgttcga tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc
    50521 ttactgatag ctatcttgat tttctctaaa cgtgcagatt atctaacagt agctaaatta
    50581 tccattgggc ctggtatctt caatatcaac gaaccagtga tgtttggtct gccaatcgta
    50641 ttgaatgcaa tcatgtttat cccattcttg attgctccag tagtagcaac agcaattgga
    50701 tggacagcta cttatcttgg cctagtcgct ccggtatcgc agcaagtcac atgggtagta
    50761 ccgccgttct tattgtcatt cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca
    50821 ttagtatgta tggtcgtgac tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa
    50881 atggaccctg ccgcaggcga acaacaataa gatgaacgtg aggagcggga cgctttaagt
    50941 ttcgttcctc ataatgaatt ttcgaggagg aactgaaatg attacaattg ccagtgcaat
    51001 gcaacaagat gaaatcaaag aattgttaga aggatacaat gaagaaggaa tgacttttac
    51061 atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca agtgcagaag atccggaagt
    51121 tgcggctaaa aaagcaaagg aactaatcaa agcacagcct tggggaacag tcctttattt
    51181 tcaatcaaca gctgtttaga aaatggttta aaaagatagg atggttgaga tgataaaaaa
    51241 cggtttattt ttatgtagta ttggttcttt gatcgtgttg tatagtttga atccacattc
    51301 gcaaaggtat gatttgtata gtatggtcac aggtcttttt ctaacaggat taggaatcta
    51361 tttcttcatg aaggggaaaa aaagagaaga agcaaaggag aaaaataact aatggaaatc
    51421 acgaaaaaat taaatattcc agcatcgttt ttttacgata aagttatgga ctctgtcctt
    51481 ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa aacagctgaa aaatttggag
    51541 tacgtcaaac aattctcgca aaataaccgg gctcgcataa aaatcgaaga agtcattgaa
    51601 aaccagtctt ataagtttcg tacatcgaca acgaagaatg aattcgtcgt tcattatcaa
    51661 atcaaaccat tggacgattc gacctgtgag gttcattaca cagaaaaaat ggagtcttat
    51721 ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg
    51781 agacgtttca aaaaaatgct agatatgatc gaagcaacgt attaaatcaa tgaaacatgg
    51841 tactatttag ttattgaaaa atgaagtaag ctagaaggaa tttttcatag gaagatagat
    51901 ctttcctgcg aatcctcact agcttttttg taggaagggg cagcaaaatg acaaacaaag
    51961 taagagagct aggtgtatct tcgattgagg aaatgtttga tttagccact tatgcattta
    52021 atggggcaga tacacctgaa aggcgagaac gttttcgtac acttgcggag aattcttgga
    52081 attacggatt ttttgatgag gaggatcggc taacaagtca agtcatggcg accccttttc
    52141 cagtgaattt ttacggacaa gagtacctaa tggccggcat tggctatgtt gcttcttatc
    52201 cagaagcaag gggacaaggc gggatcaatc agatcatgga aaagatcctt gaagattgcc
    52261 gtgatcgtaa agtaagctta tcttatctgg cgccattctc ctatcccttc tatcggcgct
    52321 atggctatga acaatcattt gataaaatca gttatcaact aaacagtcgt gatgtgccgt
    52381 ttatcaaaaa aaaatctgga aaaataaagc gtatggattt tgaagatgca aaagctgcaa
    52441 tcagaagaat ttatgaacag atgccggaaa accaacgagc tggtctgaag cgcgaagact
    52501 ggtggtatac gtataaattc aagcaaaaag gcaattacca gtttgctttg cattttgatg
    52561 ataatgagga agcaacggga tatatcgttt atcagcttgc aacgtcttcc tttattattg
    52621 cggagtgggg atttttggat catgatgctt tttgcagcct tgttcgcttc gtaagttctc
    52681 acaatggtgc ttttgaaaca tttgaatatt catgcggcta cggcggtaat gatcaaaatt
    52741 atctattaga aaatccattt gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg
    52801 tagatatttc tttgtttttg gaaaattatc catttaaaaa gagacaagct gcatttgccc
    52861 tacaaataga agaagatcat tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg
    52921 aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa
    52981 gtatccaaca tatcacgcag ctattattag ggtatcgtaa agtggaagag ttggtattcc
    53041 aagaaagaat acaggtgaca aaagaatatg aagaaacgtt aagctcaatc ttgccacagc
    53101 aacagccaat tcttaatgac tatttttgat aaaaaacggg tgatgacaat atttttgtca
    53161 ccaccctttt gtttttcatt tggcatttga aggaaaatgt taaagtaaat cctctttttt
    53221 cgtaaatttg aattttttgt tttcttttgt tttagggttt tcttttttgt ggtcttggat
    53281 tgcttctttc gtaatctttt cccgattttt tttagatgaa tctaagcgtt ctgcgtatac
    53341 tttttctttt ggcttatcat tcattggaaa ggacctccct acattatttc attctacctc
    53401 tagtttatca tctgttaagg actagtggaa acgttttgcc taacttataa gctgattttt
    53461 ttagcagata agcttgcaag taaaaaacaa acaagctaca attgatttat tgaaattgtt
    53521 cctgtagctc agtaggatag agcaatcgcc tcctaagcga taggtcgctg gttcaattcc
    53581 agtcaggaac gtataaataa cccctgtatc ctttgcggta caggggtttg ttttgtattt
    53641 gtgccaaaaa tgtgccaaac tagttatcat ttttatataa actatcgaac acagtattga
    53701 ttttttcttt atcctgatct tcaagctctc gaacaatatg aagataagta gacattgtag
    53761 tttctaaact gctatgtcct aaacgtttcg atacgctaag tatatttact ccttgataaa
    53821 gtaaaataga tgcatgtgta tgccttagtc catgcaaagt aaattgcttt tcaatcccta
    53881 attcttttaa ttttttgcgc aaatatttgc tgacggcgtt tgatgaaact aatccatttt
    53941 ttaagttgaa aaaaacaaaa ttatgagggt ttttgatttc aaaattctcg aataattctt
    54001 tttgattgat tttaaacttt tttaagagat caatcgtatg tttatcaatg gaaattttcc
    54061 tatttgaagt ttcgtttttt gtttttcccc attcatttaa tttataattc catgttttat
    54121 taatagtgat gatttgatct tcgaaatcga tatcttccca tgttaatcct agaagttcag
    54181 caaatcgcat tccagtggca ccagcaacta acacgagcat aggagaagag tactgcgcag
    54241 aaaggttttc ttctacgagt ttcattaaag atttaaattg atcataatct aaatatttat
    54301 cttcttttgg cttcaatgaa tcttttcctt tgattactgc ttttctagtt ggatcaaacg
    54361 gaattaatcc ttcttctacg gcatctttca acgacgctct aatatggtta ttaaacttaa
    54421 taacggtaga ttttacatgg tctttagcat acttatttaa aaagcgttga tatcccactc
    54481 tatctaaatc agaaatcaat accgctggca tatatttctt tatgttcatt aacgtatctt
    54541 cgtattttct ataagtgata ggagatactg ttccttcttt ataaagttgc atccaatcct
    54601 caaagtaatc tgataataat agatttttgc gatccccttt aaggcctttg ttcaagttat
    54661 attctaattc gttggcggca tcttttgctt ctccttttgt cttaaatcct gattttctta
    54721 tcttcgtgta ttttccgtca tcttttttgt atgaaatttc gtattgccaa ctatttccac
    54781 gtttgactaa tcttgccata attgaatcag ctctctttct ctgatacaat aggaactata
    54841 aagaagccca ttgtttgggt tttattattt tttgcacatc ctttttgctt gccggcgcaa
    54901 ggatgtgcta tttgtcgttt taatggtata attggatgtg tattagatca tccaatcaga
    54961 ggtgtttatt atgattaaag tgatttttaa aagaataact gaacttccag agtttagatt
    55021 atttgttgat tattggtatg tctggttttt tattgttgca ttgctgatag gtctagatgt
    55081 catattagaa agaaaactta agtaagagtt gagttaataa attgaattcc taaatgagat
    55141 attttgtagc ccatattttg tttgccgaca tcacctaata ctatggagct aatcgttttt
    55201 tctaacagac caaaatcaga taatctattt aagctaccat ctattagctc ttgtgaatag
    55261 ccagacatgc tgatatttcc agtgaaattt tcgtttccac gactcattaa gcatgctaac
    55321 aattgaatct caatcgatgt taattgattc agtacaagaa gataatactc ctcgttatcc
    55381 catgaagcgt tgttaattcg aagtaaacaa ttcttataaa gatttttata catatctatt
    55441 ttattttgtg cctttgattt ttctacctcg tcatttatcc cttcgaatat tccaatgagt
    55501 tggtccttgt tctctaagtt gaaaacatct tctggaattt gatcgcgaat gttttctaat
    55561 gattcgttta aatttttgta gaatttctcg atgcgtttga aacgcttctc gtttttagac
    55621 ccaaaataaa gtgactgcac aacgccacca atataaggaa ttgctccaat accgccttca
    55681 actattaatt caagatattc ttttttggtt agtttttcat tgctattatc catagttacc
    55741 tcctattttg tcatttcctt tacaagcatt gtgactcctg atggcactct tttgtatagg
    55801 ttttattatt gtgcacaatc taactcttag tggggcgtgg ttcatttatt ttttacttac
    55861 tttactatta atcatatccc attgagcata ggatttttct atgttcgatc ctcttgaact
    55921 aatttctcct gtaaaagtta ctttatcgcc ctttgtaaag gtatcagcat tgtttaagtc
    55981 cttcgcaaaa attacatatg aaactttagg tgttgcagaa atatcattcc aacttttatc
    56041 atcgtactct ttgtctgcta taatggctat tctagtagtc attgattcga taacagttcc
    56101 ttcaaaagta tatgttttac cactaatata tttagtaaaa gctgtatttt gctcagtagc
    56161 tggagtaata gaataataac tatctaagaa agcaccccag tcatgattat ttgtctctaa
    56221 aaattcttca aattgagtta ggcttttatt gctgctgtta gatgtagtag tttgagtatc
    56281 agatgatgag ctttcctgtt tgttattgtc atttgcctcg tttgatgagg aaagcgagaa
    56341 acctacggta gtaaaaataa tcccgataat aaggagaatc ataggtgttt tcttatttgt
    56401 ttttttgata aatcttacaa ttaacatgat taaacctata ataaataaaa caataccagt
    56461 aaaaccaaat ataccgtcca ttaatagatc ctctttctct tttgatattg aatattataa
    56521 accaagcaat tcttttttct taaaatcgaa ttcttcttga ttaataatcc cttcatctaa
    56581 caactcttta tactttttaa tttcatctgc acttgaaatt tgtgtttctt tggaattatt
    56641 atctaatata gattgttgct tctctatata tttttttaaa ttttccattt cattccaata
    56701 tttttttgta aacataacgg tgttttcatc tttcgtggcc gctaatatac ctccgcgact
    56761 ttcgtttccg cctagcaagg taaattgtat ataaccatta ctcatacctg gtttttttaa
    56821 ttgtacggct gatatatttt taaaggggat agttttttct cctttaatac cttgattaac
    56881 taaatttata aatccttttc taataattga aatagaatca tcagttactt taatcaaggt
    56941 ttttcctgga gattttatta aaatctcttt ttccattttt tcttcctcat ttctgtgata
    57001 tgattttttt tgtagaagct catatatgag gaaagagccc gtgtt
//
LOCUS       DAYYAR010000002        56728 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000002 DAYYAR010000000
VERSION     DAYYAR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.002"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(639..1142)
                     /locus_tag="ACHAT0_000063"
     CDS             complement(639..1142)
                     /locus_tag="ACHAT0_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8450044.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1501..2526
                     /locus_tag="ACHAT0_000064"
     CDS             1501..2526
                     /locus_tag="ACHAT0_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8450045.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2849..3727
                     /locus_tag="ACHAT0_000065"
     CDS             2849..3727
                     /locus_tag="ACHAT0_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM8450046.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4203..4319
                     /locus_tag="ACHAT0_000066"
     CDS             4203..4319
                     /locus_tag="ACHAT0_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8450047.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5247..5585
                     /locus_tag="ACHAT0_000067"
     CDS             5247..5585
                     /locus_tag="ACHAT0_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450048.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5679..5909
                     /locus_tag="ACHAT0_000068"
     CDS             5679..5909
                     /locus_tag="ACHAT0_000068"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8450049.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6309..6680
                     /locus_tag="ACHAT0_000069"
     CDS             6309..6680
                     /locus_tag="ACHAT0_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8450050.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6954..7646)
                     /locus_tag="ACHAT0_000070"
     CDS             complement(6954..7646)
                     /locus_tag="ACHAT0_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8450051.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7649..8434)
                     /locus_tag="ACHAT0_000071"
     CDS             complement(7649..8434)
                     /locus_tag="ACHAT0_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8450052.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8671..10071
                     /gene="lacG"
                     /locus_tag="ACHAT0_000072"
     CDS             8671..10071
                     /gene="lacG"
                     /locus_tag="ACHAT0_000072"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8450053.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10096..10413
                     /locus_tag="ACHAT0_000073"
     CDS             10096..10413
                     /locus_tag="ACHAT0_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8450054.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10426..12099
                     /locus_tag="ACHAT0_000074"
     CDS             10426..12099
                     /locus_tag="ACHAT0_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8450055.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12113..13744
                     /locus_tag="ACHAT0_000075"
     CDS             12113..13744
                     /locus_tag="ACHAT0_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8450056.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14235..15800)
                     /gene="guaA"
                     /locus_tag="ACHAT0_000076"
     CDS             complement(14235..15800)
                     /gene="guaA"
                     /locus_tag="ACHAT0_000076"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8450057.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15859..16782)
                     /gene="coaA"
                     /locus_tag="ACHAT0_000077"
     CDS             complement(15859..16782)
                     /gene="coaA"
                     /locus_tag="ACHAT0_000077"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8450058.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16978..17583
                     /locus_tag="ACHAT0_000078"
     CDS             16978..17583
                     /locus_tag="ACHAT0_000078"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8450059.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17891..18907
                     /locus_tag="ACHAT0_000079"
     CDS             17891..18907
                     /locus_tag="ACHAT0_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8450060.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            19048..20349
                     /locus_tag="ACHAT0_000080"
     CDS             19048..20349
                     /locus_tag="ACHAT0_000080"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8450061.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20395..21057
                     /gene="deoC"
                     /locus_tag="ACHAT0_000081"
     CDS             20395..21057
                     /gene="deoC"
                     /locus_tag="ACHAT0_000081"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8450062.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            21078..21476
                     /locus_tag="ACHAT0_000082"
     CDS             21078..21476
                     /locus_tag="ACHAT0_000082"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8450063.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21542..22624
                     /locus_tag="ACHAT0_000083"
     CDS             21542..22624
                     /locus_tag="ACHAT0_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8450064.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22836..24398
                     /locus_tag="ACHAT0_000084"
     CDS             22836..24398
                     /locus_tag="ACHAT0_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450065.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24391..25524
                     /locus_tag="ACHAT0_000085"
     CDS             24391..25524
                     /locus_tag="ACHAT0_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8450066.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25524..26480
                     /locus_tag="ACHAT0_000086"
     CDS             25524..26480
                     /locus_tag="ACHAT0_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8450067.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26579..27745
                     /gene="deoB"
                     /locus_tag="ACHAT0_000087"
     CDS             26579..27745
                     /gene="deoB"
                     /locus_tag="ACHAT0_000087"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8450068.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27769..28587
                     /locus_tag="ACHAT0_000088"
     CDS             27769..28587
                     /locus_tag="ACHAT0_000088"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8450069.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28604..29311
                     /gene="deoD"
                     /locus_tag="ACHAT0_000089"
     CDS             28604..29311
                     /gene="deoD"
                     /locus_tag="ACHAT0_000089"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8450070.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29609..30295)
                     /gene="gpmA"
                     /locus_tag="ACHAT0_000090"
     CDS             complement(29609..30295)
                     /gene="gpmA"
                     /locus_tag="ACHAT0_000090"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8450071.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30467..31048)
                     /locus_tag="ACHAT0_000091"
     CDS             complement(30467..31048)
                     /locus_tag="ACHAT0_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8450072.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(31181..31864)
                     /gene="rpiA"
                     /locus_tag="ACHAT0_000092"
     CDS             complement(31181..31864)
                     /gene="rpiA"
                     /locus_tag="ACHAT0_000092"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8450073.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            32183..32596
                     /gene="rpsL"
                     /locus_tag="ACHAT0_000093"
     CDS             32183..32596
                     /gene="rpsL"
                     /locus_tag="ACHAT0_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8450074.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32680..33150
                     /gene="rpsG"
                     /locus_tag="ACHAT0_000094"
     CDS             32680..33150
                     /gene="rpsG"
                     /locus_tag="ACHAT0_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8450075.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33244..35331
                     /gene="fusA"
                     /locus_tag="ACHAT0_000095"
     CDS             33244..35331
                     /gene="fusA"
                     /locus_tag="ACHAT0_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8450076.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35503..36690
                     /gene="tuf"
                     /locus_tag="ACHAT0_000096"
     CDS             35503..36690
                     /gene="tuf"
                     /locus_tag="ACHAT0_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8450077.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            37097..37405
                     /gene="rpsJ"
                     /locus_tag="ACHAT0_000097"
     CDS             37097..37405
                     /gene="rpsJ"
                     /locus_tag="ACHAT0_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8450078.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37436..38065
                     /gene="rplC"
                     /locus_tag="ACHAT0_000098"
     CDS             37436..38065
                     /gene="rplC"
                     /locus_tag="ACHAT0_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8450079.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            38093..38716
                     /gene="rplD"
                     /locus_tag="ACHAT0_000099"
     CDS             38093..38716
                     /gene="rplD"
                     /locus_tag="ACHAT0_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8450080.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38716..39006
                     /gene="rplW"
                     /locus_tag="ACHAT0_000100"
     CDS             38716..39006
                     /gene="rplW"
                     /locus_tag="ACHAT0_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8450081.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            39045..39878
                     /gene="rplB"
                     /locus_tag="ACHAT0_000101"
     CDS             39045..39878
                     /gene="rplB"
                     /locus_tag="ACHAT0_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8450082.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39921..40199
                     /gene="rpsS"
                     /locus_tag="ACHAT0_000102"
     CDS             39921..40199
                     /gene="rpsS"
                     /locus_tag="ACHAT0_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8450083.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40222..40569
                     /gene="rplV"
                     /locus_tag="ACHAT0_000103"
     CDS             40222..40569
                     /gene="rplV"
                     /locus_tag="ACHAT0_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8450084.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40583..41239
                     /gene="rpsC"
                     /locus_tag="ACHAT0_000104"
     CDS             40583..41239
                     /gene="rpsC"
                     /locus_tag="ACHAT0_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8450085.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41242..41676
                     /gene="rplP"
                     /locus_tag="ACHAT0_000105"
     CDS             41242..41676
                     /gene="rplP"
                     /locus_tag="ACHAT0_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8450086.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41666..41854
                     /gene="rpmC"
                     /locus_tag="ACHAT0_000106"
     CDS             41666..41854
                     /gene="rpmC"
                     /locus_tag="ACHAT0_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8450087.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41879..42145
                     /gene="rpsQ"
                     /locus_tag="ACHAT0_000107"
     CDS             41879..42145
                     /gene="rpsQ"
                     /locus_tag="ACHAT0_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8450088.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            42203..42571
                     /gene="rplN"
                     /locus_tag="ACHAT0_000108"
     CDS             42203..42571
                     /gene="rplN"
                     /locus_tag="ACHAT0_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8450089.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42608..42916
                     /gene="rplX"
                     /locus_tag="ACHAT0_000109"
     CDS             42608..42916
                     /gene="rplX"
                     /locus_tag="ACHAT0_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8450090.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42944..43483
                     /gene="rplE"
                     /locus_tag="ACHAT0_000110"
     CDS             42944..43483
                     /gene="rplE"
                     /locus_tag="ACHAT0_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8450091.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43502..43687
                     /locus_tag="ACHAT0_000111"
     CDS             43502..43687
                     /locus_tag="ACHAT0_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8450092.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43723..44121
                     /gene="rpsH"
                     /locus_tag="ACHAT0_000112"
     CDS             43723..44121
                     /gene="rpsH"
                     /locus_tag="ACHAT0_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8450093.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            44153..44689
                     /gene="rplF"
                     /locus_tag="ACHAT0_000113"
     CDS             44153..44689
                     /gene="rplF"
                     /locus_tag="ACHAT0_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8450094.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44850..45206
                     /gene="rplR"
                     /locus_tag="ACHAT0_000114"
     CDS             44850..45206
                     /gene="rplR"
                     /locus_tag="ACHAT0_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8450095.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45227..45727
                     /gene="rpsE"
                     /locus_tag="ACHAT0_000115"
     CDS             45227..45727
                     /gene="rpsE"
                     /locus_tag="ACHAT0_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8450096.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45742..45921
                     /gene="rpmD"
                     /locus_tag="ACHAT0_000116"
     CDS             45742..45921
                     /gene="rpmD"
                     /locus_tag="ACHAT0_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8450097.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45965..46405
                     /gene="rplO"
                     /locus_tag="ACHAT0_000117"
     CDS             45965..46405
                     /gene="rplO"
                     /locus_tag="ACHAT0_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8450098.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46405..47700
                     /gene="secY"
                     /locus_tag="ACHAT0_000118"
     CDS             46405..47700
                     /gene="secY"
                     /locus_tag="ACHAT0_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8450099.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47762..48409
                     /locus_tag="ACHAT0_000119"
     CDS             47762..48409
                     /locus_tag="ACHAT0_000119"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8450100.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48600..48818
                     /gene="infA"
                     /locus_tag="ACHAT0_000120"
     CDS             48600..48818
                     /gene="infA"
                     /locus_tag="ACHAT0_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8450101.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48851..48967
                     /gene="rpmJ"
                     /locus_tag="ACHAT0_000121"
     CDS             48851..48967
                     /gene="rpmJ"
                     /locus_tag="ACHAT0_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8450102.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48985..49350
                     /gene="rpsM"
                     /locus_tag="ACHAT0_000122"
     CDS             48985..49350
                     /gene="rpsM"
                     /locus_tag="ACHAT0_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8450103.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49378..49767
                     /gene="rpsK"
                     /locus_tag="ACHAT0_000123"
     CDS             49378..49767
                     /gene="rpsK"
                     /locus_tag="ACHAT0_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8450104.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49847..50785
                     /locus_tag="ACHAT0_000124"
     CDS             49847..50785
                     /locus_tag="ACHAT0_000124"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8450105.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50813..51193
                     /gene="rplQ"
                     /locus_tag="ACHAT0_000125"
     CDS             50813..51193
                     /gene="rplQ"
                     /locus_tag="ACHAT0_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8450106.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51465..52304
                     /locus_tag="ACHAT0_000126"
     CDS             51465..52304
                     /locus_tag="ACHAT0_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8450107.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52280..53149
                     /locus_tag="ACHAT0_000127"
     CDS             52280..53149
                     /locus_tag="ACHAT0_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8450108.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            53139..53936
                     /locus_tag="ACHAT0_000128"
     CDS             53139..53936
                     /locus_tag="ACHAT0_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8450109.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            54139..54885
                     /gene="truA"
                     /locus_tag="ACHAT0_000129"
     CDS             54139..54885
                     /gene="truA"
                     /locus_tag="ACHAT0_000129"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8450110.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            55549..55743
                     /locus_tag="ACHAT0_000130"
                     /pseudo
     CDS             55549..55743
                     /locus_tag="ACHAT0_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55931..>56728)
                     /locus_tag="ACHAT0_000131"
     CDS             complement(55931..>56728)
                     /locus_tag="ACHAT0_000131"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8450111.1"
                     /translation="NKKETTFMNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFIS
                     KGPFPSPRKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFV
                     QDENDANEIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHS
                     TEKTLYFTGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAI
                     PDSTIIAVHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT        18111 a        10136 c        12117 g        16364 t
ORIGIN      
        1 aattagtcaa agaagggcgt atagatttct tttgatagga gatatacaac agaaatgaat
       61 aaatattaac gtgttttcaa acagtataaa acggtgtggt ttttggaata aaaaaataac
      121 atatctcatt ttacactttt gttattttat acttaaaaaa taacgacaca gaaaataccg
      181 tgtctcagat ttctctacta cttctaaaaa ctactgtttt tttcctttga aatgccttca
      241 aaaagtagga tcaggcagaa tatactggca gaatcaagga gaagggagcc taaataagga
      301 gaaaaaccat agaaaatata aagtaattac ttataaaaca agctttttat agaaaaataa
      361 tatacctgag tagggtataa attaagacgg aattcctttc tagattgaga tattgactag
      421 gaagaatgtt aagattttaa gcctttaagc taaaaaaaaa ccctttttgt taaataaggg
      481 gaatatagcc tgtcagtttc aaactgcaag tcgtcctgct gcaataatat agtagattag
      541 agatgaaaat agattttttt aaaccaatga ctgaataaaa agaccgtcat agatacacat
      601 tggaatgtgt ctattacggt ctttttttac gaacgatttt attgaagacg gacagttcgg
      661 gtcaaaaata aaaaagctac gatcagaaat aagaataaaa tgtatcccaa aataatggct
      721 agacggtcta acttatttgt cgatagatgt ttgtttagaa gttgataggc ccataaacct
      781 atcagtcctc ctgttgtatt agttatcaaa tcagtgatgt ccgctacgcc aataccgaat
      841 aggaattgaa tcaattctgc caagaagctt acaaggaaaa taatccctaa ttttttagaa
      901 aaagaaagtc tcttgaaatt gacattcaaa agcaccccaa aaggaaaaaa gacaatcaag
      961 ttataagtgg tctcacgtaa taccgtcgta ttttcaaaag aaaaagtaga aaaagggacg
     1021 agattgacat tgctgtaaga ataagtgaga aattgtggaa gattaaatga tagcttgaat
     1081 aaaatcaacc ataatagaaa gaataaataa atccctaata aaaattttga tacataccgc
     1141 atagacatct cctccattta aaaagtatac catcgaaaac cgctgaaaaa tcataaaaaa
     1201 gtatgaagtt taaggaccaa cgtagttatc gtacgaaaat tagaagcttt tttttgttgg
     1261 gaatccggtt tctttaacaa agctaagata gcgagttgct tggaaaagtt gctatctttt
     1321 ttctgtcaaa acgactcgga atgttcgttt tttgttctaa tttctccaag ttgtttactt
     1381 tttgatacta agtttatgaa aaatcaagaa atagttgtta tactaggtaa tgctgacaaa
     1441 cgggattggc atttcatctt ggttcagctt tttttccaat tactaggaag gagggataga
     1501 ttgttcaaga cttattttcg tttcaataaa atacaagata atccttttcg tatcatgaat
     1561 ctgatgctac ttatctttat tgtattatgg ttaggttaca taaatcatga ttttttgatt
     1621 tcaagttttt catcattagg gatctttact tttttctatt atcaaaacat accgatgaaa
     1681 tatttgctga aacgtatgtt tctgatcggt tgcggactgt tggttgcttt cgttttgggg
     1741 atcttatcga cttatgtttt atggcttgaa ccatttgccg tagctttggt tgctttttcc
     1801 agcagattcg tgttgcgatt attccatatt tctaaaccag gtggcttgtt ttttgcgatg
     1861 ctttctgcca tgggaacgag catgcagctg ccaatcgctc aattaccgat cgtctcactt
     1921 tattttttta tgggtgtcgt ttttgccctg attgcggcag tgattacaaa attactggat
     1981 tcacggcctg agcagacaat tgaaaaagca actttaaaag aaagatttca cgaagagcct
     2041 ttagtgatca ttgattctgt tttctacagc gctgccttat ttctttccgt ttatgtcagt
     2101 cacggcttga atctgcataa tccttattgg ttgaccttgt catgtgcatc gattttgcta
     2161 gcagaaaact tggatgcgat gaagcatcga caagtccaat atctgattgg atcgatgggt
     2221 gggttatgtg tgtcggcatt cttgtcattt gtgccgttta cgcagttgca gaccattttt
     2281 ctgatcactt ttctatatgg aatcgcacaa tttctagttg ctagaaatta tgcagttgct
     2341 aatatttttc ttaatccgat ggcgttgatg ctatctacac ttattcgtgg agcatacttg
     2401 atttctttga tcgaatatcg ttttttaggg atcgtcatag gaagtttcat cggtttaggt
     2461 gttgcgtggg taatgacggt tggtttgcaa cattatttaa cggttgttcg tcaaaaatta
     2521 gaatagaagg acaaatatat gtgccaaaag aggttatgac aagatttttg tcacaacctc
     2581 taattcaagg tgttcaaaag ccagattctc agcattaagt caaattcaca caaaaatgtg
     2641 gaaaggaact ttcaaaaaaa ttttatattt attctatcaa aaaattttgt ttatgatggt
     2701 aactgaataa actttcctga gatggtcaat gctttccttg aaagtcttac agatattctt
     2761 tcttcataga aagtttaagt tttatagtta gtgtacaata aaagtatgtt tataccatat
     2821 ggtgtccgga taatcagtca tttaaacaat gaggttcaaa gatatgaaaa ggggaaattt
     2881 gatgaagaaa ataagcaatg aaatccaaat tctggcagaa actttaactc ttcccaaagt
     2941 cgttatagag caaatgattg tacaagcaaa aaacatcctt gatgaggtgt ttgagcaata
     3001 tagtcatgaa ctaatggatc ctaataaagc aaaggcgact gcaaagaaaa tagacaaagt
     3061 ctatgaagag gaaccttttg cagcattagc tatttatcta tatgcagctt ctcaaagttg
     3121 gaaaaaaatt tatcaaccat tgaagattcc aagaactatc tatttggcaa caatgaatgc
     3181 ttttactaga tttattcatg aacatttcca agtaacagga agctatcgct ttgaccgtgg
     3241 tttttggatt tggcattaca ctagtggctt gatctttcgg attggtgaat tggagtacga
     3301 aatgacatat tttccgaaag ggtacacgga tctaagatta gaaggaaagc agtgcctttc
     3361 tcttcatatt ccttccgatg cagatttatc tcagaataag ataagagata gttacgaaag
     3421 tgccattcgt ttttttgaac tttattttcc tgattaccat taccaagtga tgtacacaga
     3481 tacttggtta ctatcaccca atcttactaa atggttgaaa aaagagtcaa aaatctgtct
     3541 ttttgcagcc gattatcgat tattatcagt tgatgaacag gacgatagtg gagtcccttg
     3601 gatctttgga cgagtagatg cccaaataca tgattatcca gaaagtacga gtttacagcg
     3661 acaagcaaaa gagcaattgc ttgcaggcga gcacattggc tctggcttag gtattcttac
     3721 ttcataagtt tagtacttcg aaatagttta aaagagcgtg agacaaaagt aaagaacact
     3781 tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata
     3841 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag
     3901 ttaaacgctt ctgtcccagc ctctttttta gtgttctatc gaacacacag tacgttcaat
     3961 aggtaaacta gctataacaa tagatattaa agttaacgga caagagggcc ttttttaggt
     4021 agctaaaacg taataatgaa aatttatttc cagagatagg agtcaatcag ctagtctgat
     4081 cgactgtttt aacaagttga ctttcatttt aacttttgat accatttaaa acacaaatcg
     4141 taattgtttc gatttgtgtt ttttagcatg actacaatca aagaagaaag tgggtcgtcc
     4201 atatggaaaa aacagatatc tcaagtgcat accgccgctt gaaaagccca aatatcaaaa
     4261 cacgaaaacg tgcgctgaag atcatcaaag atgtgaaacg gaatagtggg aaaaggtaag
     4321 aagaaggaaa cttaattaaa aaaatagccg aaaaacgttt ttcggctatt ttcaataatc
     4381 ggtttttgtg taaggtttcc gaaagaactg ctgttgattt tccattttta acatatgata
     4441 aattggtaga atctttcttt tcaaattgat accattccct aattccagtt atatagttaa
     4501 ctacagaacc acaaagcacg tacgtttata aaagtattat ctgtagttat ttttcaattc
     4561 cctttcaacc tcttattttg accagtagta ttgatcactt taccttttaa cacctttgtt
     4621 cgtttttgaa acatttttag tcaagaatgg aaacgtaact acataatcaa aggttttgcg
     4681 aaatatttca tgaaatattt catgtagaag cgagcaatac ggttgcaaat aaccattatc
     4741 gtttaataag cagttacgaa agctcccaat atctctaacg ttcagctcac tccatttctt
     4801 ttcttcaaaa aaatcagtga ttcgtatctc tttctatttg tcgaaaaagt taactttaaa
     4861 aagtaacgcc gaaaaaatat gcaaaaaaat aaaaaagcag ttactattgc tagaataaca
     4921 tcaaggaata ataagttttg gttttcagat aatagtcgat aagttgtgta ttggaatttt
     4981 agatacagta ataaagtatt tttcatgaaa ttttgtgaga atcactatta caagtatctt
     5041 taataaaatt aatggttaag gaggaactta aagtagaatg taaatgaata gttgtagtca
     5101 gtcgattgtg atactagact caaagttgca ggaataagtt tttcaatttg tgattggaag
     5161 ataaaaaatg aaaaagaagg gctttttttt atactttgtt gattgtagtg tttattttcc
     5221 ctctgtttag ccaagtaaca ttttctatga ctcaaccagt ttcaagtgag gaacatgata
     5281 acctttttcg tgctacagaa gagtttaaga aatatcagga aaatattgtt ccggagactc
     5341 cattagctag tgagccaatt actgatattg atggaaacca aataggatgg tatatacagt
     5401 acgaagttat ggtggtaaat aatgactcaa tagtaaaaaa tacagtaggc ttagagctaa
     5461 caccgattga aaaggtgagt tcattgttaa cgtatattta tactgatgaa agtgacgaga
     5521 ctgagatcat tttaacggat tactcaaaaa ttgctactga taaaaagtat atattgtgga
     5581 tgtagacact agtgaaatac tttatgatat agaaattgat gaaaatttac tagatgtagc
     5641 acaagaaatc gatcatcaaa agcaggaagc tattcgatat gctggtagaa aagggaaaag
     5701 aaaatattta ttacgtgaat gtagcaaaag ttagagaaga tgaaaatgag tggaaagaat
     5761 tcaagagtcg ctactcaatt aactcgactc cgacatttac agtttacaga gaaggatcga
     5821 tagaaaaaac tgttttttgg actaaagaaa gtggaatgtc tttagctgaa gtagaagagt
     5881 ttttggatta tgttagtatg caacagtaag ggagttctta ttatgaaaaa ttttgacaca
     5941 tattatgtta ttgttttttc gatatctttg ctgttaatat tactaacttt aatgcagtat
     6001 aaagagtatc cagagtattt tattttaact tttgtatatt tagtaagtag ctacacactt
     6061 ctatataaat tcagaagttc ttctagagtt tctaaagttt gtttcttaat tagcttattg
     6121 attctataat atttttgatt tatataggaa attaaaaata ttatagaatc aacactcaga
     6181 gaacgggttg gtttttaata gatttaataa gtactgataa tgaaatttct cctggttggg
     6241 aaaacgaagt tggttaccct gtagaagaat tttttcaaga ttactttgcg gagtatgccg
     6301 ataggataat gagagattac gtaacagata aaactcaaga agtcatcaat caatcccagt
     6361 cattaaatga attatctgat aaggcgaaag aagaagcagt tgccattatt tcttcaggga
     6421 ttagtgacta tattgatatg agtggtgaaa tcaattatgg cgataaagac aaaattgtac
     6481 gcatgataaa agcttttatc aaaacttttg atgtgaaggc agatcttaag tttaatgata
     6541 cttacctagt aggaaaatta attaatatcc ttgggacaga gaaagatacg gaaacgttta
     6601 tttcctcttt gtctatgttg tttacagata agcatttttc aactcagctt ggtggagagg
     6661 aactagtgga aatcttttaa ccattacgcc cagcattaat aaaagtatat caaaaaagta
     6721 ctcctgatga tttttaagat tggtttgaga aataatgtga aaacactatt tttaatgtta
     6781 attatagata gagatgaacg cgtcatatgg ttaaaagaat gaaaaagtca aagatacact
     6841 gctatatttc actaaggata tagcagtgta tctttcatgt cgtttcactg gcagtttttt
     6901 ataaaaggag gtgtgcattt cagttgcttt tctttggtag ttagttgaat cgtttaagaa
     6961 ttaacggatg tggcagacag gtgctctgtc caaaaaccag taaaatcttt gatggttcca
     7021 tctttattta gctgtgcctg ttcaaatact gttttgtttt taccgaatgg atcaattgca
     7081 ataattgttc cgatccccgc acgtttggca gcaagtaatc ctgagtaagc atcttcgata
     7141 acaagacaat cagccggagc taagctcagt ttgtctgccg ctttaagaaa aatatccggt
     7201 tgcggtttcc cagggaaaga gccgtcgtca tagacaatct tgtcccagtc aaaccagcgg
     7261 gctaaatcga aaatatcaaa ataaaaagca acattctctt tgacagtagc cgtggcaatc
     7321 gttaatggaa tctgttggtc agtcagctgg tcaagagtcg cttcaagccc ttttgtcaac
     7381 tgaagctcgt tttcattttg caagcaaagc atacgataat agctttcttt ttcttctgac
     7441 aatacggcga tctcagaggc ggtcaaatgc tctgaaatga aatgtttcaa aatctccgta
     7501 ttcgtccgac catgaatatt ttttaaaata tcctcgtctg ataaaccatc ttttgtatga
     7561 tcatgtatca tatgcagcca agcagcttca tgcagatgag agtccaaaaa cattgttccg
     7621 ttgaaatcaa atataatccc tttcatagtt acgctcctat agtggtattt tcggttgaat
     7681 cagcaatttg cagatcgaca tattgtgcat agtgctcttg aacctcccgc gagatctggc
     7741 gatccgtaat gagcagatcg aaatcatgca aattataaaa cacataaaaa tcatctcgat
     7801 tgaatttttt attatcaacg agtagatatt tcttacggga attatttaga gcgatctgct
     7861 gtgcttctcc ttcttcggta ctgtaagtag agatcgcttc gttgtgaacg ccatttgaac
     7921 tgataaatgc ttttgtaaac tttagtcttc ttaaattgtc gttggtgatc ggtccgacaa
     7981 atgcgccggt atttttccgg aaatcaccgc caatcagtag aatttctggt ccatctgctt
     8041 gagcaagaat atcaaaaact ggataattat ttgtaatgat ccgaattttc ttgttcagta
     8101 aatgcgtcgc caatagttcg attgttgtac ctggtcctaa gaaaatcgtt tctccctcgt
     8161 gaatcaagga ggctgctgtg gcagcaattt cttttttttc ttgaatctgc agagtggctt
     8221 tttcagtgtg agaaagttct tgatctaaag tgtaggaaag actttgagcc cctccatgga
     8281 cacgcagcag cttgcccgat ttttctaatt catctaaatc tctgcgaacg gtcatatcag
     8341 aaacatctaa ttgattgatg atttcattaa cggtaaggat tcctttttta ttgaccattt
     8401 ctacgatagt aagcaatctt tcttttttca acattgtctt cacctctaat ttaaggataa
     8461 tctatcatat aaacggacaa cagtcaatct aattcgaaca aaagtaaaca tttagggtgg
     8521 tttttatgtt tgttacaata gattttattg ctgttttctg gtttttatga ttatttgtgt
     8581 ttgtttttgt tgacaaatgt tttggatgcg tttacaatgc aatcagttag aaagagcgaa
     8641 aactgattat agatttgagg agagatgtac atgcttaaat taccagaaga ttttattttt
     8701 ggcggagcca cagcagccta tcaggttgaa ggagccacta aggaaggtgg caaaggcgct
     8761 gttgcttggg atgacttttt agaggaacaa gggcgattca gtcccgatcc ggcaagtgat
     8821 ttttatcatc aatatgcaaa ggacattgaa ttgtgtgaac gttttggtgt aaatggtcta
     8881 agattatcca ttgcttggag ccgcattttt cctgatggag ctgggaaacc aaatccagaa
     8941 ggaatcgctt tttatcatcg tgtgttcgaa gaatgtaaga aaagaaacgt aacgccgttt
     9001 gtgacgttgc atcattttga tacccctaaa cgtttgtttg accatggaga ttttttgaat
     9061 cgagaaacaa tagaagcatt tgtttcctat gccatttttt gttttcatga attcaaagaa
     9121 gtcaaggtgt ggagtacctt taatgaaatc tatccagttg caaccaatca atatttgtta
     9181 ggtgttttcc caccaggaat caaatatgat tttacaaaga tcgtcgcctg tcttcataat
     9241 atgatggttg ctcatgcccg tgtagtgaac tatttcaaag aaaacgaact tccgggtgaa
     9301 attggtgtag ttcactcatt agagacgaaa tatgcggcaa cagacgcgcc ggaagataag
     9361 catgctgcct ttttagacga tgcattgtcg atccgtttct tattagatgc tacctattta
     9421 ggctattatt caactgaaac cttgactgct cttgatgaga tatgtgaagc aaatcaggcc
     9481 agctatcatt ttcctgaaga agatttcgtt gaattgaaaa aagcatccac gcgtaatgac
     9541 tacttaggga tcaatcacta ccaatgccat tttgtcaaag cttacgatgg agaaaatgca
     9601 atccatcata acggtacggg tgaaaaaggg acctctgttt ataaagtgaa gggaatcgga
     9661 gaacgtattt ataaagaagg aattcctcga actgattggg actggctgat ttatccagaa
     9721 ggtctatatg acttgttgct gcgaattaaa agcgattacc cacattacaa caaaatctat
     9781 atcactgaga acgggatggg gtataaagat cagtttgaag atggcattat tatggatcag
     9841 ccgagaatcg actatttgag agtctatttg gaatccttaa gcaaggcgat aacagccggg
     9901 gtaaatgtga aggggtattt tttgtggtcg cttatggatc ttttctcatg gacaaatggg
     9961 tataacaaaa ggtatggctt gttttatgtt gattttgaaa cccagaagcg ttacccaaaa
    10021 gaatcagctt actggtacaa gttggtcagt gaaaccaaaa cgattattta ataagtgata
    10081 atggaggaag aaaagatgaa taaagaggaa ttacaaatgc ttggttttga gatcgttgca
    10141 tattcaggcg acgctcgcag tacattattg aaattattga aagaagtaag acaaggaaac
    10201 tttgaacatg ttgattcggc gttgaaagaa gccgacgaaa atttaaccct tgcacataat
    10261 tcgcaaacaa aaattttagc ggaagaagca tctgggaaag agatggagat gggattcatt
    10321 tttatccatg ggcaggatca tttaatgacc actttattgc tgagagatct gattcaagac
    10381 tttattgtac tgtacagaca gaatcaagga taggagaaga acgctatgga tggaatcatt
    10441 aaacaaatcg aaaaagggaa accatttttt gaaaaaatct cacgtaatag ttatttagga
    10501 gccgttcgtg acggattttt aacagcgatg ccagctattt tattttcaag cattttcatt
    10561 ttgattgctg cggttcctga gatttttggt taccaatggc ctgaatctgt ctccacatgg
    10621 ctatggaaag tctataatta ttcgatgggt gttgtagggt tactcgtttc agcaaccact
    10681 gctcgctgct tggctgattc gatgaatcgt cgcttaccga aaaacaaagt aatcaacacc
    10741 acctctgtga tgctggcctc tgtggtaagt ttcctgttgt taagcgtctc tccgattgaa
    10801 ggtggatttt caacggaata tatggggaca aagggactac tggcttcctt tgtcgctgca
    10861 tttttaacag ttaatttata taagttttgt gtgctgaaag atattaccat taaaatgcct
    10921 aaagaagttc ctggaacgat ttcgcaaacc ttccgagata tttttccttt ctctttttca
    10981 gtatttgcgg ccgttttaat tgatttaggc attcgacaaa tcttccaaat ctcatttgca
    11041 gaagcaatca ttacggtgct gcagccgcta tttacagcag ctgacgggta cttaggaatc
    11101 gctattatct ggggagcgat ggcgctgttt tggtttgtcg gtgttcatgg accgtcgatc
    11161 gtggagccgg caattgcggc aattatctat gccaatgtgg aaaccaatct gcaattgttc
    11221 aattccggtg aacaagcttc taaagtactg acggtgggtt tagggaattt tgtaggaact
    11281 atgggaggaa ctggggcaac attggttgtt ccttttatct tcctactctt tgctaagtct
    11341 aagcagttga aggcagttgg gaaagcttcc tttattccag taagtttcgc tgtcaacgaa
    11401 ccattgctat ttgcggcacc catgattttg aacccttatt tttttattcc atttttgtta
    11461 acacctgttg taaacgtttg cctgttcaag ttttttgtcg atgtattggg catgaacagc
    11521 tttatgtacg ttttgccttg ggcaactcca gcaccgatcg gtttggttct aggaacgggc
    11581 atgggtatct tagcttttgt tctagcagcg gttctaatcg tcgttgactt cctgatctat
    11641 ctaccatttt gcaaagcata cgatgctgtc ttagtagaga aagaagcaca aaaagcagag
    11701 gtgatcgcaa ccgaggaagg tgcttctgcc gatgtacgtg aggaagtacc tgtggcagcg
    11761 agtcaagtta tcagcgatgc aaataaagaa atcaatgtgt tggtactttg tgctggcgca
    11821 gggacaagtg cgatgttagc taatgcttta aaaaaaggtg catctgaatc gaaagcaccg
    11881 atcagtgcaa tggcgggtgc ttatggttct cattacgaaa tgctgcagga ttttgatatg
    11941 gtggtcttag caccgcaagt caattcttac tatgatgata tcaaagaaga caccgatcgc
    12001 ttaggaatca agctggttgc gacaaaagga gcagaatata tcggattaac gagagatcct
    12061 gaaaaagctg ttcagtttat tctgaaccaa tttcaataaa ggaggagcca taatgaaaat
    12121 gggaatttgg ttgcgtaaag ctgttttatt tgcagctgtg ttaggggctt tgggggctgt
    12181 ttcaaaaact gttgaagcat ctgccagcga atttattcgc ggagcagata tttcgatcct
    12241 gtcagatatg gaaaaatctg gtgcaaaata ttatgaagac gatatttcaa aagatgcctt
    12301 agagatttta aaaaataatg gtgtcaacta tgttcgactg cgtttgtggc aggatcctta
    12361 taatagtaat ggagaaagtt atggagcagg gacaaatgat ctggaaacga cgattgacct
    12421 tgcaaaacga gcaaaaaatc tgggacttaa ggtgctgctg gattttcact acagtgattt
    12481 ttgggttgat cctgggaaac agaatcttcc taaagcatgg caaggtctga cctttgaaga
    12541 aatgaatacg gcattgtacg actatacaaa gaacgttctg tcggaaatga agcagctgga
    12601 cgtttatcct gatatggttc agatcggcaa tgaactgaat agcggaatgc tctggcctta
    12661 tggcaagtct tggggagaag gcgggggaga atttgatcga ttggctgctt ttttgaagtc
    12721 tggtatccaa gctgttcgag acactcaacc taaaacaact ccagtaatgc tccacttagc
    12781 tgatggtggc gatacaggag catttacttg gtggtttgat gaaatcacga gtcgaggtgt
    12841 gtcatttgat ttaattggag tgtcgtatta tccgtattgg cacggttcaa tgaatgaact
    12901 tcaaaccaat atgaatgcga tcagccaacg gtatggtaaa gaagtgatcg tagttgaaac
    12961 agcttatggg catacaacag cgaatgccga tactatgcca aatgcttttg gtgaagccga
    13021 agcagctgcg gggggatatg aaccaacacc gacaggacaa gcagagtatt tgctggatct
    13081 tgccgatcgg attcaggcag taccgaataa tcgcggggcg ggcttcttct attgggaacc
    13141 tctttggtac aacggaaatg tgagttgggc aacacaagca ggtatgaatt atctcagtgt
    13201 tcaatccact atgggaaacg aatgggacaa ccaagcgatg tttgattttg ctggaaacgc
    13261 cttgccaagc ttgcgtgcgt ttaaacaagc aggggcgcaa ataaatctag ttaaaaatgc
    13321 aagtttcgaa agcgatggac caacgaccag tccttccaat tggcaaacct ggtttcaaca
    13381 aggaaccgct aagggtacga tcaaaacgga agcgggcact ttttttgggg actacaaact
    13441 aactttttgg caggatcaag catacgaggc atcagcttat caaaatgtta gtggcataag
    13501 caaaggaact tacacattga gtgcctatgt aatgtctggg ggagagtttg atactaatca
    13561 gttatatatc aaaaattatg gtggagcgga actaaatact gcggttgtta aaacaagtga
    13621 atggacaaaa attacaatca aagacattcc aattacaaca ggtgtttgtg aaattggggt
    13681 ttatagcaag gcttcaggaa atgcttggac gaacgttgat tttgtttcat tggtgaaaca
    13741 atagactgcg gatcgtttcg attcaagatc ggaggttccc ttcagccaca tcgttagcgt
    13801 agaaggcagg aatcgaacct gcacctcgcc tttgccaaaa ataaccagaa acgatttcca
    13861 gtcattttta ccaaaacgag taaagcacca gacttcatct acactttgta ttatgctaaa
    13921 gagagttcaa cgatttcgta agaagcaatg aaaaaattgc aaaaagttgt gcagataaca
    13981 taaagtttgt ttaagaaagg gtaaccttag aattccgtcg tgaatatgat ttgagcactt
    14041 agatggatag aaaaaagttc tcggaaaaca gttcgttttt cgagaacttc tttttatgat
    14101 aagaatgaaa caaattaact tatattatag tctcttttgg tagggaacca tcttattttt
    14161 aatgaaaatc aagcatttta gctattgaag aatgttgatt tatagggatt ccccctccat
    14221 attatgttgg ctctttattc ccactccact gttgctggtg gtttagaagt gatgtcatac
    14281 acgatacggt tgacgtgggc tacttcgttt acgatacgta cagagatttt ttgcaagact
    14341 tcccatggga tacgggcaaa gtcagcggtc attccgtcga ttgaagttac agcacggata
    14401 ccgactgtgt agtcgtaagt acgtccgtca cccataacac cgacagagcg gatccctgga
    14461 agaacagtga aatattgcca gatatcacgg tcaagtccag cagctgcgat ttcttcacgc
    14521 aagatagcat cagattcgcg tacgatttct agcttgtctt cagtgatttc gccaagaaca
    14581 cgaataccaa gaccaggacc agggaatggc tgacgccaaa cgattgaatc aggcatgcct
    14641 aactgagtcc ctaattcacg gacttcgtct ttgaataatg tgtttaatgg ttcgatcaat
    14701 tcaaattgca tatcttctgg cagtccacct acattatggt gagatttgat cgtctgtgct
    14761 gtttctgtac cagattcgat aacgtcagta tacaacgttc cttgtgctag gaaagagacg
    14821 ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa catatacgaa ttcattccca
    14881 atgattttac gtttttgttc tgggtcagat acacctttca acttgtctaa gaagcgatct
    14941 cttgcattga ctttgatgat gttcaagccg aattttccgc ctagactttc cattacttgt
    15001 tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga tagatgtcaa ttggtcaccg
    15061 atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa cgccacctga aagaccaagc
    15121 aagacttttt tgtcaccaac ttgttcacgg attttttcta cttgcatatc gataaagtta
    15181 tccattgtcc agttaccttc acagtgacaa acatcaaagg caaagtggcg cagcaattcg
    15241 attccgtatt cggaatgacg tacttctgca tggaattgga taccataaaa attacgtgtt
    15301 gtgtcttgga tagaagcaat cggacaatct ttgcttgttc cgactgtttc aaagccttca
    15361 ggaactttag ttaccaagtc accatggctc atccatactg tttgttgttt tggtgtttct
    15421 ttaaacaaga cagcttgatc gcttgttact tctaacaagg ctttcccata ttcgcggttt
    15481 ttagcaggct ctacggaacc gccaagtttg tacgtgatca attgcatacc atagcaaatc
    15541 cctaatacag gaatacctaa attgaagatc tcaggatcga tcccaaatgc attctcatca
    15601 tatacactgt ttggtccgcc agaaagaatg attccctttg gattcatcgc ttttacttct
    15661 tctgcagtaa tgcggtggct caataattcg gaaaagactc caagatcacg aatacgtctt
    15721 gtgatcaatt ggttatactg actaccgtag tctaatacga taatcttttc aacgttcgtc
    15781 atatcggcaa cgtttgtcaa atttattacc cctatccctt taaattttat ctcaaaatag
    15841 tcaaactatt ttaaaatgct aatattttcg catgaaaatc gaatcgatga tatgattttc
    15901 agttttatgg agaattatat cggctcttcc tcgtgtaggc aagatatatt cctgtaaatt
    15961 tttcaaattc acatttttcc atacatcttt tgccatttta aaggcttctt cacgatctcc
    16021 gatagcaaaa cgataataat aattctctgg attttggaaa gccgtatcca gtaacgcacc
    16081 aaaacgatcc aaataccatt tttcgatcag cacgggatcg gcatcgacat agacagaaaa
    16141 atcaaaaaag tcgctgacgt agatttgctg attcgcaggt aattgaagcg tattgatccc
    16201 ctcaacgatc aagatatcag gctgctggat caattcgtac ttgtctggaa tgatatcata
    16261 cacatcatgt gagtaaacag gtacttttat ctcttcctga ccagatttta cacggttcaa
    16321 gaaatcgatc agcatctcca tatcataact ttcaggaaat cctttacggt ccatgattcc
    16381 acgctctttt aatatacgat ttggataaag aaatccgtca gtcgtgatca gctgaacgtt
    16441 tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt ctggctgttg tactttttcc
    16501 aacggctaca cttccggcga tcccgataat aaaaggcggt acttctacgt atttatgaag
    16561 aaataaacct ttactcaaga acaaagattc aaattctttc atataaagat aaatcagatg
    16621 agcaagaggt acatagatat ctcgaacatc ctgcatggaa atgcggtcat tcaaactttt
    16681 gatattatct agttcatctt gtgtcaaagg tgcttgccca ttacgataaa attctcgcca
    16741 ttcttctctt gccacacggt agtagttcat tggatcattc atattagcct cctgcgttct
    16801 cccatcaatg gaactatttt aacacaaaat aaacgcaaac cccaccactt aagagagatt
    16861 gccgatcttt ttgtataatc tttataatag aaagaaacgg agtcttattc ttgacttgcg
    16921 cacggataca taaaaggata gactgttgtc atgaaagaaa tgagaaggat ggaagacatg
    16981 acaaatcatt actattctga acgcccagat acggcgcacg attttgaaca atgggctttt
    17041 gagttaaaag gcagaacctt tcaatttgta acagattctg gtgtattttc acgtgaaaca
    17101 gtggattatg gttcacgtgt cctgattgat gctttcgatt gggaagagtt acctgatgaa
    17161 ggaaagatct tagatgtggg atgcgggtat ggaccgattg ggttagcttt ggcatttgcg
    17221 acacagcggt ttgttgaaat ggtcgacatt aatgaacgag cggtaagtct agcgcaagga
    17281 aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc aatcgaatat ctatgaagca
    17341 gttcatgaag aaacctatgc agctatcgta agcaatccgc cgatccgtgc tggaaaaaaa
    17401 gttgtacatg aaattttgac aggcgcatat cctcggttga agaagggcgg tacactgaca
    17461 attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa agaaaatgga agaaacattt
    17521 ggtaatgcag aaatcgtcac aaaagataaa ggatattata tcatcaaaag tgtgaaagaa
    17581 taactagctg aaaaatagtg gagcgacgaa agagtggaac tgggcgtgca tgtacgggta
    17641 cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa agaactattt tttcttcaaa
    17701 aagaaatagt aggaacagag tcgttgtttt ttacgataaa ataaataaga tacagaactt
    17761 gtatagaaga ctgccaagtc ggtagataaa ggaggttttt atcaaaaaac atactttcct
    17821 tttttctcta ttcttttctt gggggttttg gtacactgat agggaattta gatagaaggg
    17881 agccaatagg atgaagctga ccatcagaga tatcgcagaa atggcaggcg tatcagtaac
    17941 aactgtctca cagattttga ataacaaagg aagcagattt agtgataaga caagaaaaaa
    18001 agtgttgtcg atcgtgaatg aacatcatta taagcccgat tatttcgctt caaatgtgat
    18061 caatcgacat tcaaagacca tcggaatgat cgtccctgat gtcactgatt tcttcttctc
    18121 caaactgatc gaaggtgtag agagctattt gaatcctttg ggctatgtga tcatgttatg
    18181 caattctcgt catagccaag aaaatgagat caagtattta caagagcttt cccaccgctc
    18241 agtggacgga atcttgctgg ccacaccaaa tattttacct gagaaatatg cattagacag
    18301 cggtttttat caaaaaatgc caatcatttt aatcgatcgc ggattgaatc gtcgggataa
    18361 cggacggctt atcgtaaagg aatatgaggg cgcctatcaa gctgtttcgc tgtttattga
    18421 aaatggccat acgaagatcg gcatgctgaa ggaaaccact ggatactatc aattagaaga
    18481 acgtttcaac ggatatcgtc atgcgctgaa agatcatggc ctgcctttta atgggaaatt
    18541 tgttgaacat ggagagttga ctgtccaagg tggatatact gcatcaaaaa aattactcaa
    18601 acataaagat atcacggcta ttttttgcgg gaatgatgcc atggcgatcg gatgctatca
    18661 ggcgatcgat gaattgggca aaaaaatccc agaagatatc tcagtcatag gattcgatgg
    18721 attaaagctg tcagaataca tgattccgca attgacgaca gtgcagcagc caagttttga
    18781 tattggattt tatgctgcac gtttcctgat cgatacgatc gaatttcctc aaagaaaagt
    18841 tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag agcgtaaaag tattgacaaa
    18901 gggctaactc ggcggtatat tgcttatgta agcatttaca aaggctagtt agtgcttttt
    18961 ttatttttag taaaccagtt tactaaagtg gtttaccttt cctaaaaata atgtataata
    19021 ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg atctgattga aaaaaagagg
    19081 gatggaaacg agctgtcgaa agaggaaatc gagtacatcg tcacgaacta tacgaatgga
    19141 aagattccag attatcaagt cagtgcatta ttgatggcaa ttttttatca agacatgaca
    19201 aatgaagaaa tcacgaactt aacattggcg attgctaatt ctggtgatgt catcgatttg
    19261 agttctcttg aaggaataaa ggtagataag cattcgacag gtggcgttgg tgacacgacc
    19321 acattgattt tagcccccct agtagctagt gtcggagtga cagtggctaa aatgtctgga
    19381 cgcggtctag gttacacagg tggtacacta gacaagctag aagcaattcc cggatttcag
    19441 atcgagcttt cggatgaaga atttgtacgc attgttaatg aaagtaaagt agcggtaatt
    19501 ggtcaatcag gaaacttggc accggcggac aaaaaattgt atgccttgcg tgacgtcaca
    19561 gcgaccgttg attctcttcc gctgattgcc agttcgatta tgagtaaaaa aatcgcggca
    19621 ggagcagatg caatcgtttt ggatgtgacc actggagacg gtgctttcat gaagaacatc
    19681 gaagatgcaa gacgtctggc aaagaccatg acgagcattg ggaaactggc taaccgagaa
    19741 acagtggcgg ttatctcaga tatgtcagaa cctcttggcg aagctatcgg aaatagtcta
    19801 gaagttgtcg aggcaatcga aacattacaa ggaaatggtc cagaagatct agtagaaatg
    19861 tgctacgcct taggaagcca aatggttgtt cttgctggca aagctaagac aaccgatgaa
    19921 gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag cgttagcaaa attcaaagaa
    19981 atgatccaaa atcaaggggg agacccagcg atcgtggaac atccagaacg aattttgact
    20041 gcacgctaca caatggagct tcctgctaaa caaagcggag ttgtgtcaaa aatcgtggca
    20101 aatgaattag gaattgctgc aatgatgcta ggtgctgggc gtaaaaccaa agaagaagat
    20161 attgatcacg ctgttggatt gaaactgcat aaaaaaattg gtgacactgt gacaaaaggt
    20221 gagtcactat tgacgatcta tagtaacgac gaagaaatca cttctgtcat tgaattatta
    20281 tataaaaata ttgaaattgg tgaatcggca atgaagccaa cgttgattca tgacatcatc
    20341 acagaataaa ggaaaaaagc ggactttacc gttttaaaga aaaggggaaa agaaatggaa
    20401 ctaaaccgaa tgatcgacca tactattttg aaagcagacg catctaaaga agctgtgatg
    20461 caaattatcg aagaagcaaa aaaataccat ttttattctg tctgcatcaa tccagcatgg
    20521 gttgcgctag cgaaagaaca attacaaggt gagccagtag ccgtatgtac agtaatcggg
    20581 tttcctctag gagcaaacac acccgagaca aaagcatttg aaacaacaga tgccatcaat
    20641 aacggtgcag atgaagtcga tatggtcatc aatatcggtg ctttgaaatc aaaggatgat
    20701 caacaagtcc aaaaagatat cgaagcagtt gttgaagcag caaaagatcg tgcgcttgtc
    20761 aaagttatta ttgaaacatc gttattagat agagaagaaa tcatccgagc ttgcgaaata
    20821 gcaaaagctg caggcgctga ttttgtcaaa acatcaactg gtttctcaac aggcggagca
    20881 aaggtagagg atgtccgttt gatgcgtgaa actgtaggac cagaaatggg tgtcaaagct
    20941 tccggcggta tccataacga agaagaagca atggcaatga tcgaagctgg tgctacaaga
    21001 atcggaacaa gcgcaggagt agcaatcgta tctggttcaa ctggtgaagg ctattaattt
    21061 tttcaaagga gaagctcatg gcagaactca aacaagaatg gatcaacaca gctattgaag
    21121 cgttagataa agcctatgtt ccttattccc attttccggt aggcgcttgt ctcgtaacgg
    21181 aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc atcatttgga ttaacgaatt
    21241 gtgccgagcg cacagctttt ttcaaagcag tatctgaagg agaaaaaaca tttacgcatc
    21301 tagtcgtagc ggggcacacc cccgatccta tctctccttg tggtgcatgt cgacaagtaa
    21361 tggcagaatt ctgttcccct gatatgccgg ttacgctagt aggagacaat ggtgtgacga
    21421 aggcaacgac cgttcgtgca ctgctgcctt atgcatttac tgaaaaagat ctataactca
    21481 tgttcattca gtcagctgac tgttgaaaaa taaaacaatt attttaggag gctttacaga
    21541 aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca ctagcagcag ggttattttt
    21601 aggtgcatgc ggcaacaacg gctctactga ttcatccggt ggcaaaagct catcagacac
    21661 aacaactgca gcattgatca cagacacagg tggtgtagac gaccgttcat tcaaccagtc
    21721 agcttgggaa ggactagaaa aatgggggaa agatcaagga ctttcacgag gaaatgatgg
    21781 cttccaatat ttccaatctt caaatgaatc agattacatc ccaaatattg accaagcatt
    21841 gaatgctgga ttcaaaacga tttttggtat tggttataaa ctaaaaccag cgatccaaga
    21901 acaagcaaca aacaatacag gtacgaactt tgttattatc gatgatgtca tcgatggatt
    21961 agataacgtt gtatctgcaa ctttcaaaga caacgaagct tcttatcttg caggtgttgc
    22021 agcagcttat acgactgaaa caaacgtagt cggctttatc ggcggtgtaa aaggtgaagt
    22081 aatcgatcgt tttgatgctg gattcaaagc cggtgttgat gcaggtgcga aagaattagg
    22141 aaaagaaatc aaagtattga atcaatatgc tggtgacttc tctgctccag ataaaggacg
    22201 ttcaattgct caaggtatgt atgcacaaaa cgcagatatc atcttccatg cttcaggcgg
    22261 tacaggtaac ggtgtattcc aagaagcaaa atcattgaac gaatcaggcg acaaaaaagt
    22321 ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc gaatacacat taaacggcga
    22381 aaagaaaaac ttcacattga catctacact gaaagcagtt ggaacagtag tagaagattt
    22441 ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa cacactgttt atggattgaa
    22501 agaagacggt gttggtctaa cagaaggaca actttctgac gaagcgaaaa aagcagttga
    22561 tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt ccagaaactc ctgaagaaaa
    22621 ctaaaaccat ttctatagcc ttgttagaca catgtttgac aaggctatgg tttgattaga
    22681 acaagaattg ttcatttatc caagctgcct tgtgacagtt tggataaggg aacaactccc
    22741 tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc gtgaatctta gtcaatcgtt
    22801 gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt tcaagaaaat attgtcatag
    22861 aaatgcagaa tattaccaaa gaatttggaa cattcaaggc aaatgacaac atcaatttac
    22921 aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa cggtgctggc aaatctacat
    22981 tgatgaacgt gctttccgga ttattagagc cgacatcagg gaaaattttg atgcgtggga
    23041 aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt agggattggg atggtccatc
    23101 agcactttat gcttgttgat gcctttactg taacagaaaa catcgtgttg ggaagcgagc
    23161 ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa agagatccaa aaagtttctg
    23221 aacagtatgg attgtcagtc aacccagatg cttacgttcg tgatatttca gttggaatgg
    23281 aacaacgggt agaaatttta aaaacacttt accgaggagc agatgtactg atttttgatg
    23341 agccgacagc tgtattgacc cctcaagaaa ttgatgaatt aatcgtgatc atgaaggaat
    23401 tagtcaaaga aggcaagtcg atcattttga ttacgcataa gttagatgaa atcaaagcag
    23461 tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat cggtacagtc aacgttaaag
    23521 acgtaacctc acaacaatta gccgatatga tggttggaag aacggtttca ttcaaaacga
    23581 tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat tgaaaatcta gtggtgaaag
    23641 aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga ggttcgtgct ggcgaagtac
    23701 ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt gatccaagct ttgactggtt
    23761 tgcgaaaggc agaaagcgga catatcaagc taaaagggga agacatcact aataaaaaac
    23821 cccgaaaaat cactgaacat ggtgtaggac acgtgccaga agaccgtcat aaatacgggt
    23881 tagtcctaga tatgacatta tctgaaaata tcgcactaca aacctatcat caaaaacctt
    23941 acagtaaaaa cggcatgctg aattattcag taatgaatga acatgccaga gaattgatcg
    24001 aagaatatga tgttcgaaca acgaatgaac ttgttcccgc aaaagcttta tcaggcggaa
    24061 atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga tcctgatctg ttgattgttg
    24121 caaatccaac tcgtggactg gatgtaggtg cgatcgaatt tattcataaa cgtctgatcg
    24181 aacaaaggga caaatacaaa gcagtgttac tgatcagttt cgaattagaa gaaattttaa
    24241 atgtttcgga tcgtattgct gttatccatg aaggagaaat tgtcggtatc gttgatccga
    24301 aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg ctatacctta gaagaagcac
    24361 gaaaagagtt agaaaagggg gctggcgcac atgaatgatc gcagcgaaaa attccgtaat
    24421 ttattagtgc ctgtactttc agttgtttta ggattgatct taggcgcgat attgatggct
    24481 gcttttggtt ttcagcccat ccaaggttat tcttctatgc tagatgcagc ccttggttcg
    24541 caaagaggga taggtgaaac gttaaggcaa gcaacgccgt tgatttttac agcattaggt
    24601 ttttctgtag ctaactcagc tgggtttttc aatattgggc tttctggaca agcactttgc
    24661 gggtggatcg tcagtgtctg ggtggcactt gcgtttccag atctgccaaa agttgttttg
    24721 cttccgttat gcgtgatctt aggtgcctta gctggtgcga tggcagcggc tgttccaggt
    24781 ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa caatcatgat gaactatatc
    24841 ttattatacg taagcacatt tgctttacat gaagtgatgc ctgaaagcta tcgctcaagt
    24901 ttggactctt ccaatatgat cagtcaaaat gcctcactgc ggttaccatg gttaacacaa
    24961 gcttttggcg gctctcgcgt aaactcagga ttatttttag caatcatcgc gcttattttc
    25021 gtttggctgc tgatgaagaa aacgacttta ggatttgaaa tccgttctgt tggattgaat
    25081 ccttttgctt ctgagtatgc aggaatgagt agtaaacgta cgatcgtttt gtcgatggtc
    25141 atttctggcg ctttggcagg gcttggcggt gtggttgaag gattaggaac gtatcaaaac
    25201 ttctttgttc aaacgacttc gctatctatt ggatttgacg gaatggctgt ttccttgctt
    25261 ggttctggat cagcaatcgg tattctttta tcagcgatct tgttcagtat tttgaagatc
    25321 ggtggacttg gcatgcagac aggagcaggt gttccgtttg agatcgtcaa tgtatcgatt
    25381 gctttgatta tcttctttgt cgggatcaac ttcctgattc gtttcgtcat ggctaaagtc
    25441 ttccaaggga aaaaacaaga agagatcgtt gcgacaatag aatcgaaacc aacgaatcaa
    25501 aaccatgaag ggggcgaact ataatgtcga ctatcgaatt aattgccttg atccttactt
    25561 ctacactggt ttattcaaca ccgctaattc taacgtcact aggcggaact ttttctgaac
    25621 gaagcgggat cgtaaacgta gggctagagg gaatcatggt catgggtgct tttagttcca
    25681 tcgttttcaa cttgacgatg agtccatcac ttggttctat gacaccatgg atcgctattt
    25741 tagtgggtgg cgtcgtaggt atcctatttt ctctgattca cgcagtagca acagtcagtt
    25801 tgcgtgctga ccatattatc agcgggacag taattaatct gatggcacct gctttaggtg
    25861 ttttcttgat caaagcatgg tatggtaaag gtcaaactga taacatcacg cagaatctgg
    25921 ggtatttttc tttcccagga ttagcagata ttccggtcat tggaccaatt ttctttaaaa
    25981 atacattctt acctgcttat tgtgctattt tgattgctgt cctagcttgg gtgatcatct
    26041 tcaagacgaa attcggcttg cgcctccgtt cagttggtga aaatcctcaa gctgccgaca
    26101 cattaggtat caatgtatat gctatgcgtt attcaggcgt actgatttct ggcttgttag
    26161 gcggtatggg cggtgctgtt tttgctcagt cgatttccgg taacttttca gctggtacga
    26221 tcgttggaca agggttcatc tccatggctg ctatgatctt tggtaagtgg aatcctttag
    26281 gggcaatggg tgcctcattg ttctttggtt ttgctcaaag cttgagtatc attggcgcac
    26341 aattaccagt tatttcttcg attccgccag tattgctgca aattgctcca tatttgctga
    26401 caatcattgt gctggttgtt ttcttaggga aagcttctgg tccaaaagca aacgggaaga
    26461 actatatcaa atcaaaataa tcttttagcg aataaaaagg gtgggttacg ggaagtgttt
    26521 ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa aagaaagaag gaataatcat
    26581 gtttaaacgc gtacatctga tcgtcatgga ttcagtagga attggagaag caccagatgc
    26641 cgataagttc ggtgatgttg gttctgatac attaggacac attgcgaaag aagcaggatt
    26701 gaacgttcca catttagagc agcttggttt aggaacgatc cgtcctcttg aaggtgtgga
    26761 atcagtgaaa gatcatcaag gctatgcgac gaaattagaa gaagtttctg tagggaaaga
    26821 tacgatgaca ggccactggg aaatcatggg attgaatatc aaaactccat ttcgtgtgtt
    26881 tcctaatgga ttccctgatg atctgatcaa acaaatcgaa gaattttctg gccgtaaagt
    26941 cgtatgtaac aaaccttata gcggaacagc tgttattgac gattacggtg aacaccaaat
    27001 gaaaacaggc gatttaattg tatatacttc tgccgatcct gtccttcaga tcgcggcaca
    27061 tgaagaaatc attcctttag aagaattata tcggatttgc caattcgttc gtgatataac
    27121 aaaagatgat ccgtatatga ttggccgaat cattgcacgt ccgtacgtgg gtgaaccagg
    27181 agacttcaca cgaacaagca atcgccatga ttatgcgttg aatccattcg gacacacagt
    27241 ccttgattct ttgaaagaag caggaaagga cgtgattgct gttggaaaga tcaacgatat
    27301 tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa agcaatatgg atggcgttga
    27361 ccagctgctg aaagttatga aaaacgattt ttcaggaatc agctttacca atttagtaga
    27421 ttttgatgca ttatacggtc atcgtcggga tgtagtcggc tatgcgcatg cgattgaaga
    27481 atttgactta cgaattccag aattgcttga agcgatggaa gaagacgact tgctaatgat
    27541 tacagccgat cacggaaacg atccgacgtt tactggtacc gatcatacgc gagaatatgt
    27601 accgttattg gtttacagta aaaaaatgaa agaacaaggg gcattgcctc aaggatatta
    27661 ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt tcttcgactg aaaatggcga
    27721 aagcttcttg aaattattaa aataatcttt ttaaatagga gaaagcaaat gaccaagtta
    27781 agcgagatgt tacaagaaac gacccagttt atccttgata aaggagtaaa agaaattgat
    27841 tttggtttga ttctaggttc aggattaggt gaattagcag atgaaatcga agatgctgta
    27901 atgatttctt atgatgagat cccgttcttt cctgtttcga cagtagttgg gcatgcagga
    27961 aagctagttt atgggtctct ggcaggcaag aaagtgttag ccatgcaagg gagattccac
    28021 tactatgaag gtcattccat gcaaacagtg acctatccag tacgtgtgat ggccggacta
    28081 ggggcccatt cagtcattgt gactaatgca tgtggtggtg tcaacgaatc atttgtacca
    28141 ggagatttga tgctgattac cgatcatatc aattttactg gtgaaaatcc tttgatcggt
    28201 ccgaatgaag atgcaatggg ccctagattt ccagatatga gtcaagctta tacgcctgac
    28261 tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt tggatcttaa agaaggcgta
    28321 tatatgggct attctggacc aacttatgaa acgcctgcgg agatccgaat gtctcggaca
    28381 ataggagccg atgcagtagg gatgtcgact gttccagaag tcatcgtagc agctcacagc
    28441 ggattaaaag tgttaggaat ctcttgtatt accaacttag cagcaggtat gcaagcgaat
    28501 ctgagccatg aagaagttgt agaaacaact cagagagtca aacaatcttt taaagcattg
    28561 gttaaagaaa cattgacatt attgtaatag gaggaacaat aaaatgagtg tgcatattga
    28621 agcgaaagaa ggcgagatcg ccgataagat tttacttccg ggagatcctt tgcgagctaa
    28681 atatattgcg gagacttttt tggaagatcc agtttgttac aatcaggtac gcggtatgtt
    28741 aggctttacc ggcaaatata aaggagaacg tatttctgtt caaggaacag gaatgggaat
    28801 gccatcagcc accatctatg cccatgaatt gatccagtcg tatggtgtaa agaaattgat
    28861 tcgagtaggt acatgcggtg cattgtcaaa agacgtccat gttcgtgatt tagtactagc
    28921 acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat ttccaagcct ttcattttcc
    28981 tccaatcagt gatttcaatc tattactgaa agcatatgag atagctaaag aaaagggcta
    29041 tacggttcat gtaggaaatg ttctttcgga agattctttc tacaaagatg atttgacaga
    29101 gacgttccaa ttagctgaac ttggcgtttt aggagtggaa atggaagcag cggctcttta
    29161 ttacttaggc gcgaaatacc atgtgcagac attaagtttg atgaccgtta gcgaccattt
    29221 gatcaccggt gaagaaacaa cagctgcaga acgtcaatct acgttcaatg aaatgatcga
    29281 agtaggattg gaaacagcga tagcagaata agaaggaaat gaaaaagctg ccctgcatta
    29341 agtaataaga acatccaagc aaaaatagct cctcatgttt ttgacttggc tggacttatt
    29401 attgcaaatg taagcttttg aacaccgttt accaagaggt tgtgaatctg ccgtttaact
    29461 ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt
    29521 gtgaccgaga agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg
    29581 tgtcacagtc ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt
    29641 catcatttaa ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt
    29701 cgtcagagat accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta
    29761 ctaaaacagt tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag
    29821 cgcgttctaa tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg
    29881 cgtaacgacg gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag
    29941 tatcgtaaga acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt
    30001 ttttgttcaa accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt
    30061 gtggtaccca aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag
    30121 ttaaaacaga agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc
    30181 cttcttttgc ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt
    30241 ggttcaatgc attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta
    30301 gctctccttt taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat
    30361 tttttggaag agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga
    30421 atataaaaaa acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt
    30481 gctcgatttc tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc
    30541 attttgaaga aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt
    30601 tgatcgtttt tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat
    30661 ctaatccaac taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca
    30721 ttaattggaa atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca
    30781 agtgcagccc gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc
    30841 ctaatcgatg aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg
    30901 actccaaatg aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt
    30961 cttgacaacc aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg
    31021 ttgtgcgtgc ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact
    31081 ggatataaca gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt
    31141 tccgctgtct ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt
    31201 caagaatctc aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa
    31261 gcccatgttc tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt
    31321 cgatgacttc tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc
    31381 cgttttcgtc tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct
    31441 gttgggagcc ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta
    31501 atttagaaga atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga
    31561 aaagcaaagc agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac
    31621 cgtctattgt caaatcgact acggggactt catcaatact ctttaacggt attcctaatt
    31681 tttccgcttg ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt
    31741 cacgcatacg gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac
    31801 ctacgatcat cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat
    31861 tcatctttgc gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt
    31921 gatttacggt aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt
    31981 gcatctcttg ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca
    32041 ggtttctgtg aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca
    32101 ttgcatttat tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat
    32161 cgtgggaagg aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa
    32221 atcaaaggtg gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa
    32281 aactcaaact aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat
    32341 gacacctaaa aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt
    32401 aattgaagtt actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt
    32461 attattacgc ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg
    32521 tgctttagat actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa
    32581 aaaacctaaa gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag
    32641 taattactat gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt
    32701 gcaaaacgtg atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc
    32761 aaccgtgtaa tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt
    32821 gacttgatca aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa
    32881 aacgttcaac ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta
    32941 ccagttgaag ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac
    33001 gctcgcttgc gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct
    33061 gcaaacaaca caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc
    33121 aaccgtgcgt ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag
    33181 tcgacctcag atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg
    33241 agaatgacca gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc
    33301 gatgctggta aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa
    33361 attggtgaaa ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt
    33421 ggtattacga tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc
    33481 atcgacacac caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt
    33541 gatggtgctg taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt
    33601 tggcgtcaag caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag
    33661 atcggtgctg acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct
    33721 cacccaatcc aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta
    33781 acaatgaagg ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt
    33841 cctgaagaat accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct
    33901 gaaactgatg aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag
    33961 ttaaaagaag gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt
    34021 tcagcattta agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca
    34081 tcaccgttag atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa
    34141 cgtcctgcag acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca
    34201 ttcgtaggtc gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac
    34261 gtacaaaacg caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc
    34321 aactctcgtt cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg
    34381 aaagacacaa ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc
    34441 atggaattcc cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa
    34501 gataaaatgg gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa
    34561 acaaacgtgg aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc
    34621 atcgttgacc gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta
    34681 tcttaccgtg aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct
    34741 ggtggtaaag gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct
    34801 ggtttcgaat ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct
    34861 gttgaaactg gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta
    34921 gatatcaaag ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc
    34981 ttccgtgttg ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc
    35041 cttgaaccaa tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg
    35101 ggtcacgtaa ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa
    35161 caagttgttc gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt
    35221 tctgctactc aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca
    35281 aaatcagtac aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa
    35341 accggatagt cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt
    35401 aaaataatta tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag
    35461 acaaataaaa aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt
    35521 gaccgttcta aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact
    35581 acattaacag ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct
    35641 tacgatcaaa tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct
    35701 cacgttgagt atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg
    35761 gattacgtta aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt
    35821 tctgctgctg acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt
    35881 ggtgttcctt acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta
    35941 ctagaattag ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac
    36001 gatgttcctg tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa
    36061 gaaaaaattc ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac
    36121 aacgacaaac cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact
    36181 gttgctacag gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt
    36241 ggtattgctg aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg
    36301 ttagactacg ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa
    36361 gacatccaac gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc
    36421 tctgcagaag tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact
    36481 aactaccgtc cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca
    36541 gaaggaactg aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac
    36601 ccaatcgcta tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt
    36661 gccggtgtcg ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt
    36721 tattcggacg taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt
    36781 tattgcagga tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag
    36841 agtcagtatt ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct
    36901 tgctttgtga gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac
    36961 agacgtaatc agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc
    37021 tttgccacga acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg
    37081 cgaaggaggg aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca
    37141 ccgtatttta gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag
    37201 cgtgtctggt ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac
    37261 tcataaatac aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga
    37321 cattgtgaac ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg
    37381 tgtaaacatc gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac
    37441 caaaggaatc ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt
    37501 aattccagta actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga
    37561 aacggatggc tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa
    37621 caaacctgcg aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga
    37681 attcaaaaat gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt
    37741 ccaagcagga gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt
    37801 tatcaaacgc cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg
    37861 tcctgggtca atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg
    37921 ccgtatgggt ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga
    37981 aagaaatgtt atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat
    38041 caaatcagct gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa
    38101 tgtagcatta ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat
    38161 cttcggaatt gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc
    38221 attaagacaa ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa
    38281 accatggcgt caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg
    38341 gcgtggaggt ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa
    38401 aaaagttcgt cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt
    38461 ggtagcaatc gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct
    38521 tgctaactta tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc
    38581 tgctttatca gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt
    38641 gctagacgtt gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga
    38701 ggaggtgctt gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa
    38761 tcaatgcttg ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa
    38821 actttagtaa aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc
    38881 gtgaacgtac gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt
    38941 cgcaaagcga tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct
    39001 gaataagcac taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt
    39061 acaaacctac ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa
    39121 catcaacacc tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca
    39181 acggccgcat cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg
    39241 acttcaaacg taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa
    39301 accgttcagc taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag
    39361 cacctaaagg actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag
    39421 tcggaaatgc tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa
    39481 tgaaacctgg taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg
    39541 gtaaagaagg caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag
    39601 ctgcatgccg tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg
    39661 gtaaagctgg acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga
    39721 accctaatga tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac
    39781 cagtttctcc ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat
    39841 cagacaaact tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac
    39901 attttgagag gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc
    39961 atttaatgaa aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt
    40021 ggtcacgccg ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg
    40081 gacgtaaaca tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat
    40141 ttgcacctac aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat
    40201 ttgaggggag gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa
    40261 acagttcgca cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt
    40321 gtcgcagatg caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa
    40381 aaagtattga tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac
    40441 ttggtagtaa gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt
    40501 gcaaaaggat cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca
    40561 gaaaaataag gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg
    40621 catcatccgc gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca
    40681 cgaagattta agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac
    40741 agttgaaatc gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg
    40801 tatggttatt ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt
    40861 aactggtaaa agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa
    40921 attagtaggt gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca
    40981 aaaacaagcc atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc
    41041 tggtcgtcta aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc
    41101 tcttcatact ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg
    41161 aaaactagga gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac
    41221 tgagaaagga gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc
    41281 cgcggaaaaa tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt
    41341 ttgcaagctg ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca
    41401 atgactcgtt acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct
    41461 tatactgcca aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg
    41521 gttgcaccag taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta
    41581 gctcgtgaag cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa
    41641 cgtgaggaaa tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc
    41701 cgaaatgctt gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt
    41761 agcaacaggt caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg
    41821 catcaaaact gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat
    41881 gactgaagga agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga
    41941 taaaacaatt accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat
    42001 gaactattct aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat
    42061 cgtgagaatt atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt
    42121 agtcgaagaa gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa
    42181 atctgaaagg aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa
    42241 ctcaggcgca cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc
    42301 taacatcggt gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa
    42361 aaaaggtgac gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga
    42421 cggttcatat attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc
    42481 tcgtggaaca cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa
    42541 gatcgtttca ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga
    42601 aacagttatg tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa
    42661 caaagaaggc gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt
    42721 aaatgtcgtt aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga
    42781 gatggaagcg ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg
    42841 ccgagttggc tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga
    42901 agttttagat aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga
    42961 aaaatatctt aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat
    43021 gcaaacacct aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa
    43081 cgctaaaaac ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat
    43141 gattacaaaa gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg
    43201 tgcaaaagtt acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt
    43261 ttcactacca cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg
    43321 aaactatact ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt
    43381 agataaagta cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc
    43441 tcgtgagttg ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta
    43501 cgtggctaaa aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc
    43561 ttatactcgt tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg
    43621 ccgtatttgc ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag
    43681 ctggtaaaaa gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca
    43741 attgcagatt ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa
    43801 gtacctgcat caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt
    43861 cgtgatgttg aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt
    43921 ggtaaaaacg gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt
    43981 gcttatgtca aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt
    44041 tcgacttctg aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa
    44101 gttatcgctt acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg
    44161 tatcggaaat aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa
    44221 cgttacagtt aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat
    44281 gaatatcgaa ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac
    44341 aattcatgga acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt
    44401 ccaaaaagcg ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact
    44461 tgtattgaac gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt
    44521 tgaagtacca gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga
    44581 attagctgca aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg
    44641 ttatgttggc gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa
    44701 aattgcatag aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa
    44761 tacattttta cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt
    44821 agtacaataa aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac
    44881 gtcaaaagag acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct
    44941 tgaacgtttt tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg
    45001 taacgctagc aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag
    45061 cagcacaagc tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag
    45121 tagtctttga ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg
    45181 ctcgcgaaaa tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga
    45241 tccaaaacat ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt
    45301 taaaggtgga cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca
    45361 cgtagggttt ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga
    45421 agatgcgaag aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt
    45481 aatcggtgct tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt
    45541 tgctgctggt ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc
    45601 taaatcttta ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa
    45661 acaattgaaa cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat
    45721 cggataagga ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc
    45781 ggacgtcctc aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt
    45841 tcagtggtta agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta
    45901 gtggacgttg aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa
    45961 acctatgaaa cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt
    46021 tggtcgtggt acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa
    46081 agctcgttca ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg
    46141 tcgtttacca aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct
    46201 tgatgtattg aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc
    46261 tggaatcgtg aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa
    46321 gaaattaact gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc
    46381 tggtggatca atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa
    46441 gacattagat cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca
    46501 catattactg taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt
    46561 ttcaacatgt tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg
    46621 ggggtttcac catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg
    46681 cctcgatttg ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg
    46741 actcgatatc tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc
    46801 aatcaatacg ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc
    46861 ggtttgattc taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa
    46921 aaaggtatcg gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct
    46981 gaaagtatca gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg
    47041 aaatctgcta tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta
    47101 acatttttcc aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc
    47161 gcgcctacaa gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc
    47221 tttgctagtt cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc
    47281 ggttctgaaa actggtacaa agtgatgatg gagatcttta actataatac agcacctggt
    47341 gctacgattt atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta
    47401 aaccctgaga aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc
    47461 cctggtaagg ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt
    47521 gcagtcttcc ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt
    47581 ccacaatcaa tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa
    47641 actgcgaaac agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa
    47701 gaaagcgtgt tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac
    47761 aatgaacctc attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat
    47821 cattgatgct tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca
    47881 aaacgaaaca gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc
    47941 tgatgaagta acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg
    48001 cttcttatta gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct
    48061 aaaagatttg aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt
    48121 ggtcgaacgt ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat
    48181 tttcaaccct acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca
    48241 aagagaagat gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag
    48301 tgaaccaatc ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg
    48361 tgaaatcgat gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc
    48421 gtagccgact tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg
    48481 tcagtccgac ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac
    48541 tgcagtttcc gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg
    48601 tggcaaaaga agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa
    48661 tgtttaaagt tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc
    48721 gtatgcatta catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg
    48781 atcttaaccg tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg
    48841 aggtattatc atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat
    48901 tcgtcgtaaa ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca
    48961 aggataattg gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat
    49021 aaacgtgtag tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag
    49081 atcttagctg atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa
    49141 acagatgcta tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa
    49201 gtgaacttga acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc
    49261 cgtggattgc ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca
    49321 gctcgcgcta tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg
    49381 gcagcaaaaa aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca
    49441 catatccatt ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca
    49501 ttagcgtggt catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt
    49561 gccgctcaaa tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact
    49621 gttgaagtaa cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa
    49681 gctacaggtt tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc
    49741 cgccctccaa aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac
    49801 gtcattgaac aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga
    49861 aaaaccaaga atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc
    49921 tcttgaaaga gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc
    49981 tttgccaggt gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac
    50041 catcaaaggt gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct
    50101 gaaaatgtat ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt
    50161 aactgctggc gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat
    50221 ctgtagtgtc tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg
    50281 ttatgttcaa gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga
    50341 ttcaatctac acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca
    50401 ccgtgaagat tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt
    50461 ggaagcaatg agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt
    50521 aactgatgaa gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa
    50581 aatgttggaa atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa
    50641 acgtgcagga atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa
    50701 agtccgtaac ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg
    50761 tttaggttta cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta
    50821 tcgtaaacta ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga
    50881 tttattaatc aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac
    50941 tgcagaaaaa atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc
    51001 ggctttcgta cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga
    51061 atcagcttta caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg
    51121 atacacacgc attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt
    51181 agaacttgtc taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta
    51241 tgatgttgga agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct
    51301 tggggagtaa gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag
    51361 atatgtttga aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt
    51421 tttgatagtg ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg
    51481 aattagaaaa gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg
    51541 tctcctttac aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa
    51601 aatcgacact tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag
    51661 tgggaaatca aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg
    51721 tttttcaaaa tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg
    51781 gtcttgaaaa tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg
    51841 agaaagttag aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa
    51901 aacaacgtgt tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg
    51961 aagcaaccag tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga
    52021 tcaaagaaga aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta
    52081 atgccaatcg gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag
    52141 aaattttttc tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa
    52201 aactgaagag cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag
    52261 aaaggatggt ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc
    52321 gaatacacca tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag
    52381 ttatactgca ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa
    52441 tgcgcttgtt aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga
    52501 tacggataat aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc
    52561 agaagcacag ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt
    52621 tggtgtcagc gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct
    52681 ggatgaaagc tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt
    52741 agcaatcgct ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc
    52801 aggacttgat ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga
    52861 acagcagatc actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga
    52921 ttatgtctat gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt
    52981 tcaaaacatt gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc
    53041 agaagaatta atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt
    53101 agcagacgct atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg
    53161 gtcgttatat gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag
    53221 ccagttttta ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt
    53281 tggcattttt cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc
    53341 gaggcattcg cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta
    53401 caagtggcgg agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga
    53461 tgaacggcct ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca
    53521 cgcttactac accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga
    53581 aagccgtacg gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg
    53641 tgcctacatt gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg
    53701 attttggtga aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt
    53761 tatttgtcag cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct
    53821 acaaaggtgg ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga
    53881 tggttatcat tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt
    53941 tcagtgggcc gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag
    54001 tgtatgacaa gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat
    54061 ctgaaaagaa agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg
    54121 aacaaaggag agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat
    54181 tttaatggat tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca
    54241 ttacaaaaaa tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc
    54301 ggagtacatg cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa
    54361 cgaatgcgtt tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa
    54421 attgtttcag aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga
    54481 gtggatatag ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac
    54541 cctcttgatt tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc
    54601 acctcttttt gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct
    54661 aagatggagg taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc
    54721 ttgtataaga tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg
    54781 cctgaagata gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca
    54841 gcacatcctg aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa
    54901 gtggtcagct tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa
    54961 atttcggctt attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa
    55021 agctgccctg catcaagtaa taagaacaac caaacaaaaa tagctcgtca tatttttcgt
    55081 ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg
    55141 aaatcagcgt tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    55201 gccgtttgtt gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg
    55261 tgagcctgtc atttaactct gagtcacaag taacaatttc aaaaaacagc ttctcatctt
    55321 tgatgaatat cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta
    55381 ttttttctgt aaccaaaaaa cgatggaacg ttgttttgat tgcccttaat gaggacagtc
    55441 ggaacaggtt tgtcattttt atatttaaat ggcacaaatt tttctaatta tttgtcattt
    55501 agcgctgaaa atttttgcta tttatctaaa aaagtgataa ctacttaaga tgaagcagaa
    55561 tatttacgcc atacggcata caaccgcgaa aaatataaaa aaaagaaaac gattgaacgc
    55621 gtttttgcag tcgcaaagga aaagcatgga atgcgctaca tcaagtatcg agacttggaa
    55681 aacaatcagg tgtatacgct gattgttttt gctgcaatga atctgaaaaa gttgtccact
    55741 tggcagttgg aacatatgtc ttcatatctt ttattataca aaaatagcaa aagcaccagt
    55801 ctacactctt agtaaagagg aaactgatcc ttttgtctac attcttaaag tacttaaact
    55861 aagtacttta ttttactctt taggattgat acagtagcat ctatcccttt attaataatc
    55921 ttttattttt ttaaataaag atgttgtctc catcttcagg cactaaaact ttttcggaaa
    55981 ttcctttttc tttattgaaa gactttaatt cttctcttga cagtccccaa tggtttacag
    56041 cttccatatg aacggcaata attgtggaat ctggtattgc tttatgcaat tcatataaat
    56101 cgtctttccc cataataaga gattctccaa ctaaaaaatg attatctcca gcatttgcta
    56161 ctatgatttc tggattatac tgatcaatag tttgtttaac agcttcatac caaactgtat
    56221 ctcctgtaaa gtatagtgtc ttctcagtcg aatgtttgaa aacaataccg caaacattac
    56281 ctacataatt caagatttct cctctgccat gtttgccttt tgttttaacc aattgtatag
    56341 attcaaaaac tgtgtcttct cgtagtacct ccacattttt aaaaccatat agtcgaattt
    56401 cattcgcatc attttcatct tgcacaaata attttatttc tttaggtagg acctctttcg
    56461 ctatatcgtc ccaatgatct agatgtaaat gtgttacaat aacagcatca ataccgttga
    56521 taatatcttc gactggcatt ggcaaatcaa ctagaggatt catttgatct tttcttggtg
    56581 atggaaaagg acctttcgaa ataaatggtg gaagtgttcc tttttccgct aacattggat
    56641 caattaaaaa ttttttacct ccatagtcta agatagctgt tgcattacga atgtgatgta
    56701 tattcataaa cgttgtctcc tttttgtt
//
LOCUS       DAYYAR010000003        55196 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000003 DAYYAR010000000
VERSION     DAYYAR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.003"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..232)
                     /locus_tag="ACHAT0_000132"
     CDS             complement(<1..232)
                     /locus_tag="ACHAT0_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450112.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEV"
     gene            complement(239..787)
                     /locus_tag="ACHAT0_000133"
     CDS             complement(239..787)
                     /locus_tag="ACHAT0_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8450113.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(832..1164)
                     /locus_tag="ACHAT0_000134"
     CDS             complement(832..1164)
                     /locus_tag="ACHAT0_000134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450114.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1178..1435)
                     /locus_tag="ACHAT0_000135"
     CDS             complement(1178..1435)
                     /locus_tag="ACHAT0_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450115.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1630..2172)
                     /locus_tag="ACHAT0_000136"
     CDS             complement(1630..2172)
                     /locus_tag="ACHAT0_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8450116.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2193..3284)
                     /locus_tag="ACHAT0_000137"
     CDS             complement(2193..3284)
                     /locus_tag="ACHAT0_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8450117.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3292..5502)
                     /locus_tag="ACHAT0_000138"
     CDS             complement(3292..5502)
                     /locus_tag="ACHAT0_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8450118.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5518..8046)
                     /locus_tag="ACHAT0_000139"
     CDS             complement(5518..8046)
                     /locus_tag="ACHAT0_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8450119.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8098..8508)
                     /locus_tag="ACHAT0_000140"
     CDS             complement(8098..8508)
                     /locus_tag="ACHAT0_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8450120.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8521..8739)
                     /locus_tag="ACHAT0_000141"
     CDS             complement(8521..8739)
                     /locus_tag="ACHAT0_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8450121.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8736..9728)
                     /locus_tag="ACHAT0_000142"
     CDS             complement(8736..9728)
                     /locus_tag="ACHAT0_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8450122.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9807..10718)
                     /locus_tag="ACHAT0_000143"
     CDS             complement(9807..10718)
                     /locus_tag="ACHAT0_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8450123.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10737..11081)
                     /locus_tag="ACHAT0_000144"
     CDS             complement(10737..11081)
                     /locus_tag="ACHAT0_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450124.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11101..11379)
                     /locus_tag="ACHAT0_000145"
     CDS             complement(11101..11379)
                     /locus_tag="ACHAT0_000145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450125.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11738..12238)
                     /locus_tag="ACHAT0_000146"
     CDS             complement(11738..12238)
                     /locus_tag="ACHAT0_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450126.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12239..12784)
                     /locus_tag="ACHAT0_000147"
     CDS             complement(12239..12784)
                     /locus_tag="ACHAT0_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450127.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12781..14037)
                     /locus_tag="ACHAT0_000148"
     CDS             complement(12781..14037)
                     /locus_tag="ACHAT0_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8450128.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14398..14709)
                     /locus_tag="ACHAT0_000149"
     CDS             complement(14398..14709)
                     /locus_tag="ACHAT0_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450129.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14732..16207)
                     /locus_tag="ACHAT0_000150"
     CDS             complement(14732..16207)
                     /locus_tag="ACHAT0_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8450130.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16656..17108)
                     /locus_tag="ACHAT0_000151"
     CDS             complement(16656..17108)
                     /locus_tag="ACHAT0_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450131.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17110..17475)
                     /locus_tag="ACHAT0_000152"
     CDS             complement(17110..17475)
                     /locus_tag="ACHAT0_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450132.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17574..21857)
                     /locus_tag="ACHAT0_000153"
     CDS             complement(17574..21857)
                     /locus_tag="ACHAT0_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8450133.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22004..22243)
                     /locus_tag="ACHAT0_000154"
     CDS             complement(22004..22243)
                     /locus_tag="ACHAT0_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450134.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22333..22575)
                     /locus_tag="ACHAT0_000155"
                     /pseudo
     CDS             complement(22333..22575)
                     /locus_tag="ACHAT0_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22688..22924)
                     /locus_tag="ACHAT0_000156"
     CDS             complement(22688..22924)
                     /locus_tag="ACHAT0_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450135.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(23935..24845)
                     /locus_tag="ACHAT0_000157"
                     /pseudo
     CDS             complement(23935..24845)
                     /locus_tag="ACHAT0_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(25041..26876)
                     /gene="lepA"
                     /locus_tag="ACHAT0_000158"
     CDS             complement(25041..26876)
                     /gene="lepA"
                     /locus_tag="ACHAT0_000158"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8450136.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            27023..28243
                     /locus_tag="ACHAT0_000159"
     CDS             27023..28243
                     /locus_tag="ACHAT0_000159"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8450137.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28263..28757
                     /locus_tag="ACHAT0_000160"
     CDS             28263..28757
                     /locus_tag="ACHAT0_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8450138.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            28735..29421
                     /locus_tag="ACHAT0_000161"
     CDS             28735..29421
                     /locus_tag="ACHAT0_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450139.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(29572..32181)
                     /gene="clpB"
                     /locus_tag="ACHAT0_000162"
     CDS             complement(29572..32181)
                     /gene="clpB"
                     /locus_tag="ACHAT0_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8450140.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(32432..33478)
                     /locus_tag="ACHAT0_000163"
     CDS             complement(32432..33478)
                     /locus_tag="ACHAT0_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8450141.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(33489..33761)
                     /locus_tag="ACHAT0_000164"
     CDS             complement(33489..33761)
                     /locus_tag="ACHAT0_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8450142.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(33796..34704)
                     /locus_tag="ACHAT0_000165"
     CDS             complement(33796..34704)
                     /locus_tag="ACHAT0_000165"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8450143.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(34836..36068)
                     /gene="pepT"
                     /locus_tag="ACHAT0_000166"
     CDS             complement(34836..36068)
                     /gene="pepT"
                     /locus_tag="ACHAT0_000166"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8450144.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(36083..37204)
                     /locus_tag="ACHAT0_000167"
     CDS             complement(36083..37204)
                     /locus_tag="ACHAT0_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8450145.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(37201..37902)
                     /locus_tag="ACHAT0_000168"
     CDS             complement(37201..37902)
                     /locus_tag="ACHAT0_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8450146.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(38046..38486)
                     /locus_tag="ACHAT0_000169"
     CDS             complement(38046..38486)
                     /locus_tag="ACHAT0_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8450147.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(38615..39541)
                     /gene="dnaI"
                     /locus_tag="ACHAT0_000170"
     CDS             complement(38615..39541)
                     /gene="dnaI"
                     /locus_tag="ACHAT0_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8450148.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(39541..40914)
                     /locus_tag="ACHAT0_000171"
     CDS             complement(39541..40914)
                     /locus_tag="ACHAT0_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8450149.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(40937..41428)
                     /gene="nrdR"
                     /locus_tag="ACHAT0_000172"
     CDS             complement(40937..41428)
                     /gene="nrdR"
                     /locus_tag="ACHAT0_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8450150.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(41740..42072)
                     /locus_tag="ACHAT0_000173"
     CDS             complement(41740..42072)
                     /locus_tag="ACHAT0_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8450151.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(42124..42753)
                     /gene="coaE"
                     /locus_tag="ACHAT0_000174"
     CDS             complement(42124..42753)
                     /gene="coaE"
                     /locus_tag="ACHAT0_000174"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8450152.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            complement(42717..43553)
                     /gene="mutM"
                     /locus_tag="ACHAT0_000175"
     CDS             complement(42717..43553)
                     /gene="mutM"
                     /locus_tag="ACHAT0_000175"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8450153.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(43598..46243)
                     /gene="polA"
                     /locus_tag="ACHAT0_000176"
     CDS             complement(43598..46243)
                     /gene="polA"
                     /locus_tag="ACHAT0_000176"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8450154.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(46369..47052)
                     /locus_tag="ACHAT0_000177"
     CDS             complement(46369..47052)
                     /locus_tag="ACHAT0_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8450155.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(47045..47530)
                     /locus_tag="ACHAT0_000178"
     CDS             complement(47045..47530)
                     /locus_tag="ACHAT0_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8450156.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(47837..49171)
                     /gene="murC"
                     /locus_tag="ACHAT0_000179"
     CDS             complement(47837..49171)
                     /gene="murC"
                     /locus_tag="ACHAT0_000179"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8450157.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(49302..50063)
                     /locus_tag="ACHAT0_000180"
     CDS             complement(49302..50063)
                     /locus_tag="ACHAT0_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8450158.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(50079..50561)
                     /locus_tag="ACHAT0_000181"
     CDS             complement(50079..50561)
                     /locus_tag="ACHAT0_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8450159.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(50581..52761)
                     /locus_tag="ACHAT0_000182"
     CDS             complement(50581..52761)
                     /locus_tag="ACHAT0_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8450160.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(52905..53636)
                     /locus_tag="ACHAT0_000183"
     CDS             complement(52905..53636)
                     /locus_tag="ACHAT0_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8450161.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(53705..54646)
                     /locus_tag="ACHAT0_000184"
     CDS             complement(53705..54646)
                     /locus_tag="ACHAT0_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8450162.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(54826..55194)
                     /locus_tag="ACHAT0_000185"
     CDS             complement(54826..55194)
                     /locus_tag="ACHAT0_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8450163.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT        15563 a        11885 c         8986 g        18762 t
ORIGIN      
        1 acacttcatt tttacttgtt attttttcat tgactataac tagttcatct ggtaaaaaaa
       61 ttaaccaaca aacatcgaca aacagtaaat gatataatcc atttccacct ccaagcatat
      121 tcaatactgg tgaacatgcc ttagaaacac gaatatagtt atggtttaaa tagatatttt
      181 ctttaaacat tgcttgttta aaagccatat gctcttgttc taaacttttc ataaactttt
      241 actctaatgc acagctagca gcagctgctg caattcctgc tacaatattt accttagcta
      301 actctttagc tgcagctttt cctaattgtt tttctgcaat ttttaataat aaatccgcag
      361 ctttttcaaa attaaaagct tttaatgctt caataaaatc tccctgcgta cctatagccc
      421 aagccactcc tcctccagga agagaattaa tggctacaca tccagcatat cgtttccaat
      481 caactttctc cattctagga acaataatat gttcataaaa gactcttgct gctcttcctt
      541 gttcgttatc ttgactgatt cgttcacgaa tagcaccaaa atcctttata tggtatacac
      601 catgttcgtc taaaaaacca actgtatcaa aataaaaagt taattcatct actaatttat
      661 tctttatttc taaatcaact gtattcactt tttctgtggc atacacactt gttccagtac
      721 taacaaaagt acttaaacct aataagaaaa cacctaaaac accaaatatt ttaattattc
      781 ttttcataat ttctcctcca taaaaaataa gttacttttt aaatatcttg tttatgaatt
      841 cattccccaa aggcctttta agattgatac agcagtccgt tcacttcttt tagacttgtc
      901 atacaagtct tttaaatgat cccatatcag actagtttgt ctatctagct tcaatccatt
      961 aaacgctaat gaacggttca ttttttctat tactttagat aaagctacag aaatttttat
     1021 accttcacaa cgcgcatctt ggatttcaat aatagtatca tcaattagtt tttgaatttc
     1081 ttgcctatca acatattcaa tcttagaaag agaatctttt aaagctacta atgattcaac
     1141 gatttctttt ttctcttcag acattttctt cctccttcta gtgacccgta aagccatgta
     1201 gagcagagtc cattgaacca gtaattacac tatctacaac gccattccaa tttactctac
     1261 attttccatt tttcagattt gtcggtccat acggatagcc tttttgacat tcaatgttcg
     1321 gattatacca gataggatta taccattcag ccgcagaagc agattcagtc gatacaaaca
     1381 atcccacacc aacaaatcca atacacatca cactatagcc aattagtttt ttcatctcgt
     1441 atccatctcc taaaaaatag aatagttctt atagttattc ataagacata ttttttccgt
     1501 ttttgtttta tcctttctgt cctataacaa cttttttcaa gtaaagaatg acatagtttt
     1561 tcttaaaaaa acagtctgtt tttttctatt tcttcggtgt ataataaata tgagtgttca
     1621 ttttttattt taatacgcac tccgtacata ataaatcttg atcgcatcaa tcttttgagc
     1681 ttcttgatct actttcaatt gaagtaatac aaattgagta accttattca ttgtttcttc
     1741 tccaaataac aaatattgat ccggattctc tatatcttgt gtcacctggt aaagtttccc
     1801 agttgttttc atttgtacat gcgggtctac atcaatacta ttgtctttaa tacaacgatc
     1861 tgtcatatag tccctcacag cttgattgcg tttataaaca tctgtgaaat tactccattt
     1921 tctagcaaaa tcttttaaga aattttttgt catttcatct atttgcattt gttgcgctgt
     1981 ctcgtcttct aatgaaccaa tttgactgtt actttcagta ctttgaagag ttccacttgt
     2041 ttcttttact gcttcgattg tttcttctgt cgttctcaat tgtgtttttt gtttttcaga
     2101 agaaaaccaa tgacaaccag taagacctat caaacataac caactaaata ctaaaattat
     2161 cccttttttc acgtttctag tcactgcctt tcttatttca ccttacgaac cagactacga
     2221 atttttgttt gattgtacgt gcgttgatac cgagccataa tccgtccttg ctcggcattc
     2281 tgttccacac tagagaaatg ttgtccgcca tatgaaacat ctgttataat tccggtgtga
     2341 ccatagattc caggagacaa tgcaccacct gcttgccaat tcaccacatc tcctgcttgt
     2401 aaatcacttg gttttgggtt catgattact tgccaaccat aggttgtcca gttataatct
     2461 tcccctatta acatcgcaaa catgtgtcca gaacccatta actgtgggcc acctaattga
     2521 gatacatacc atgccgtacc accataacat tccccattat ttacggatcg tccaatgatc
     2581 cgatccaaaa ccttcgcatt gcctttactt tttcctgcaa taagcgaagt tttttttgtt
     2641 ggtaggctat tccaatgctt gatcgcaaaa gtatcatatt ccgttaagtt ataggacgtt
     2701 atgatactca ttgctcctcg ataatacgga ctcccttcgg taatacctgc tgccgcatag
     2761 cctccacgac ctagaatcaa taaacaactt ccaggatctt tattgcccac acattgatta
     2821 aagttgcttt ggctcgccac cataaagcca taatagtcat aacaagcttt taagttcgga
     2881 aacttcatat agaaaccacc ttcagaacca ccaattccac gtgcagaacc ttgtgggtag
     2941 ggacaaccag gaaaccaggt aatcccaaaa aagttcgtat cgtttcttcc agattgtgac
     3001 tgtccataag ctgattcata gcctaattga gaaagaatca cactcggtaa taactgatac
     3061 tttacagctg caccaactgc tgcttgcgca tgttcatctg tgattggacc acgtccttta
     3121 atctctgaaa aaatcggtaa tccttcggta tattccccat taaaagcgac agctagacct
     3181 gtagcttctc ctccatgctc agaaagttct cctgaaaaaa agaatggcaa tagaaaaaag
     3241 ggaagaataa agaaaagaag acaacctaga caccctttct tcatttcttc ctcatccttt
     3301 cttcgataat ggtttttgga ctgttcgtcc ttttggtaaa gtaaaatcag ttcctggttt
     3361 tgcgtccaaa atcggcagtg ttttaggatc cttttctatt ttttccgatt gctctgttgg
     3421 cttatctaaa gaagaaaatg caggttgtaa cgattttggt gcggaaatcg acggtttact
     3481 ttcagacgaa cgttgccccg gttgttgcac agaacgattt cctgatagag tggctaccga
     3541 cgaattagga acagagtatt ggttcaataa ctgctgcatt gctggataac tcccattttt
     3601 cgcctgattt ttcacttggc gaatctgttg tgaaagagaa ccagcagctg ttaattgtgg
     3661 cacgggtact tttccagaag gcaacggaat cgattgacca acaaaagcga tacctttatt
     3721 taacaatggt tttctttgat tattcccttg ttgcttactc aattgtgcca atgattgtac
     3781 ctgttgtttt ggtcttactg aattcatgga cgaatttgga atcaaaccat ttgaaagacc
     3841 agaattccac tctgttttcg atttggtaga agacatttca ggctctaccc catgataacg
     3901 aacatgacga ccactataag gcaaggcttg atttgaacgc ccacctagtt caaagggatt
     3961 aaccacttgc ctccctttta ccgattcttg tggtgtacgt ttttgttgaa acttttgaaa
     4021 tccatgtatc gctgcagaag cgagtggatt cgttgctcga tacacattcc ctgctaatcc
     4081 tgcacctttg attgccaaat tttgtccagc agttgatagt tgaccaggca gttgtttcat
     4141 ttgagacaaa gaatgacttg ctatgttttt cgtgttgttt atagtttgat ctgcagctcc
     4201 taaccctagt ttctttaata aatctttccg ttttacaatg agtaaaattg ataggagtgc
     4261 tttgacaaac atatttccta aaaaaccaaa aacagtattc attggaatta aatcgtctac
     4321 tatgttatag atcaaaaatg ccaccaaaat aatcacactg taaaaagatt tttgaaacat
     4381 caagcccaaa aaggacttta aggtattcat gagcatataa tcaaatactg gaacaaaaga
     4441 agcgagtaat gaaaaaggaa tcacaagtaa taaacacaac accaacattt gaaagataaa
     4501 acgaatcatg ccaatcacta caagtggtaa tccaatactt agattaagca atggcaccat
     4561 agaaccaacg actgctttat aaacagcttt agttggttga atatagacac gataagcctt
     4621 ctttttccct ctatcttctc ctgcagcagt ggtcactttt gtctcaatat cagataattc
     4681 ttcacttgac gcttccttgg ctagaaattc ttgtggttca atctctgctt gatacaattc
     4741 ttctaaattt gttgtcccat aatttaacaa tatatacgga tcaacaacag ccgctttata
     4801 ataaagttgg cgcacttttt gaatggcttc cttttgtgtc gttggcacta aaacaccatt
     4861 gacatctttt gtcgccaatt catctagact atttgtagca gaaaaaataa agccttctac
     4921 ctccacggac aaatcattta accctttggt aaaagcttct cctttactag gtacgttact
     4981 accatagcca aaccaaacca ttgaaaaaat gacaacgaag agaaatcgaa taaacaccat
     5041 ttttgcgtaa tgtttccctc taaatatacg aagagtaaat agatataaca catacaagac
     5101 gacaatcccc gcaccaatag gatcgtatat ttttgaataa atagtatccg aaaaagtgaa
     5161 aaactggtga attaactcgt tcattgcagc tccttcatag agcttctcaa tgacaaaatc
     5221 aaggccttgc caaatcaatt tacttaaact aaagaacata ttcgtcatca tttttagaaa
     5281 atctgtgcca tttgccccaa ataacatttt gcctaatgaa aattcactat cagataaata
     5341 agaagaaaag gtatcaatat gaaatttccc aatctcatag atcgattgat ccacctgctt
     5401 ttctgccgca tacaccaatc caccaacaaa aagcagtccg aatccaatcg ttaaaaaaaa
     5461 caaaacgcac catttctttt ccttatgctt tatataagcc attgtttcac ctcatttcta
     5521 ttgcatgatt aacgtatcct gttcttctac ttgagagtga cttgtttttt ccgttgtttg
     5581 taatgcagcg gtcatttctt caaacaaact gtgtacattg agttttccta cacgtccata
     5641 gggatctaac atccaacact gtcctttaat catgtggctc atttcagcta agttcgcttc
     5701 atttacttcc aaacgtaaat gattcaaaat cttttctcgc tcactgtctt catcaaaagc
     5761 aaagacgaca ccaaaattcc ctttttcttt atctgaacct aagtcaccaa taaattgtgt
     5821 atctaacact aattgattca tctgggaacg ccccacacgt gccattgctg caataatact
     5881 acgtcctgcg ttcgaattat taaataccca tgcttctgta aaataaatcg ctgttttctt
     5941 actactatct cgttttccaa acatttcaca aaaacgacct aaactaatca tcaaacacaa
     6001 agactttcga tccgcatctg tatatagttc tggtcggaca ttgtcatttg gtaatttcaa
     6061 atcttggatc tctaaaatcg ttgttttggc attaaagtct aatcccgggt tttctccata
     6121 agaaaatcct aaacgtaaaa tcgaatcttc taccgtcaag cgtaaaaact caccatattc
     6181 tcgaacctct ttttgttcca tttgttctaa atagtcgacc acatgcatca agccaacttg
     6241 ttgtccggct tctttttgaa ctaaaacttc ttggattccc tttaataccg ccccttttaa
     6301 aaggtgattg gtacctaacg gacttaactc atcaatcata tcctgggcta cttgtttcgc
     6361 atctactcgg gataaacaaa caattggatc taatacacca taattacggg gatttgtagc
     6421 atctaacgtg acataactca ttttttgcaa atgttctaca aacaaagggt aatgtttctg
     6481 ataatacggg tcttgaatga gtttcgtaaa ccatttgcgc ttttctcctt tgggatccac
     6541 aaataaactt tgtacattca taaacgaact atacaaaaag agcaagccaa ctaaaaaggt
     6601 cttcccttta ccggtttttc cagttactgc tacatgaggc gcatcaaaca acgcaccttt
     6661 gattccttcc gcaatggcta gaagattcaa aaagacaaaa cgatttgacg cataaattaa
     6721 ttcttctaat ggtgtttttt ctcctcgaga accaccatcc cctaatgtat cttgccgacc
     6781 aatataccag ccggattcca tacctaattc tgatactgct gaaaacaacc attcgccaac
     6841 tgcttctaca gttgttcggt gcttccaatg taactgccgt cccaatggtt gccctaataa
     6901 acatttatag aacaattctc gttgatcatt ttttgcacgg tggatatgga taatcgtatg
     6961 aagttgttga ataaccgttg cactattttg acgaacttct tcaaccgttt gaccgtatac
     7021 aataaaacat gcgatccaat cgacgacagg ttgcccatta tctactcgtt gctttaaatg
     7081 ttttactaat cctcgaacag ttaataagcg ttcactaggt tcttcatcat tttgaaccaa
     7141 ttccttatgc tctgttttga accgttccga tagccaattc agttttaaac caatacctcc
     7201 ctgtcctttc aagcgtggat agtgaatttt cattcgcaat tctgctggga aagaaagatc
     7261 ctgagcaaat tgaaatagat cacaatttct catattttca ggtgtttccc caataggcaa
     7321 caatgctaaa taactactcc ctaactcatc ttctaattga agaaggcctt tatgttcctt
     7381 cggtatacgt acatacgttc cttgattgtc tttcagtact tcccaattat tttgttcttc
     7441 tactttgtga gccattcctc gaatatattg cagacgattg agatacaaca gctctttttt
     7501 tactaaggga cgaatagaaa agctataaag taattgtgtc acttctttta cataaggatc
     7561 actttcagaa aacaaagttt ccaaatcatc tacttctaat ccaaataagc gtgctaaact
     7621 tcctgctaaa tctaaccaga ttagatttat tacatcatga aaactctcca ccgttgcttt
     7681 ttctttgact ttgattccta aaataaatga aggacgatag acttgacctt ctcgttccaa
     7741 caatgtcact gtttcttttg cataatacgc accaattgct tgactatcgg gagaaaatcc
     7801 ctttcctaat gtgtcaaact tcgtccgtaa attcattgtt cgtggataca tcaccaactc
     7861 cacatcttca taatctgcta aaagcaacag taaacgagcc atcatttctt ttatttcctg
     7921 tgtctttttt ggtgtgtttt ttgaaattgt tcgtggaaaa acatgatagt aggcccagat
     7981 acaatcctct ccatttaaca aacaattttt cttgaatgct tttactggtt cttttacaag
     8041 caccaatgaa tttcacctcc caataaaaaa ggaacaacca gttgattgtc cctttttcta
     8101 atcctctaaa taaacatact tattcacaaa aagtaattct tccaatgcat tgtttgatac
     8161 cttgtcccag ttataataca cacaatcacc attcatcata cgaatcatat aacaaaacca
     8221 atcccataaa taaaaatgca ctttttttcc atcaggtttt agttttgcca gtgctaacac
     8281 acataaatag gggagtccta tataaaaaac aactcctgct ggtggaaaaa aagaaatgac
     8341 ccaaccaata aatgaacgta aaaataaata ttcaaagatt acgaccaaca aaaaagttgc
     8401 tgcattgact aatgcaactg gaaatggaag acgccaattg cttgtaatct gttgtacgcg
     8461 ataaggttcc gcataaacaa tcgaataatc ataaggttcc caatccactt cactatcctc
     8521 ctaatccata aataattttt caatgatttt accaaacgca tcaaatacac gtgttggatc
     8581 atttaagaag aaataaactg cggcacctgc tgcgatagat attaatgcat ttcctacttt
     8641 tgatttcata aaatttcgca atcccagata aacaaccacc attattaaaa aatagccacc
     8701 ttgcgttaat ataaaatcta caattccctt cagtgtcatt tccgttcttc tcctttccta
     8761 tccaaccaat tatgtgttaa tttttcaaca taatactgat ttgttttttt cgtaatgaca
     8821 atcgtcattt gttctttttg taaaagtgac gtatcaccat ctcgaaagac gaccgttgct
     8881 tttacaataa aaggtttttt cgtttcttta taacgcaata ctttcatctc tacttgatca
     8941 actttagctg tttgattgag tccttcaggt tctttcatca tgtatgacat atcttttgca
     9001 ttgtttttcg catacttatc taagaaatct tccacaaatc cttcaacatc tttcgcttcc
     9061 gacgcatcaa ccgtttcgta taatgtgtca tcataagtaa tcattcgttg tttttctgta
     9121 ttctttggta gtgccgtaaa ataaggataa gcaataatac gacagccctt gtttgaaata
     9181 gaaaagggaa gattgagtac ttgtgtgatt gtcacacttg tttctttctt actctctgga
     9241 ttcatcgagc gaatatcata agttacttta taactagcag ttggaatggt ttcaatttct
     9301 tttaatccaa taaacgaagc agcagttaat tttcgaacac ctgaaacact tcctggttct
     9361 tgaataaagt tttgtgcata aaactctttt tttaatgttt cttgacgttg ttgttcttgt
     9421 tcatgattat cgaatgtatt catatacaaa gcaacaaaac gattcataaa ggattcaatt
     9481 tcaggtgtgt acgttaattc tgaccgactt tgtacttctt tcttttgtaa ttgttggatt
     9541 tcatctttca aaaacgacac ttgaaccttc gttttcatgg attgaaaaaa tgccaagccc
     9601 cctgaaaaac aaagaaataa aaccaaacac caaatccatt tcactgcttt ttttgaatgg
     9661 attttctttt gcttcggtac tttctctgta taaacttttt ctttccgccg tagttgtcgt
     9721 tttttcataa gctactcctt ctagtatgtt tttactagta cgtacgattg acaacgataa
     9781 gcttgttttt ctgaaaaata gtccattcaa ttaccctcat tttctattcc ttttctcttt
     9841 cttttatatc caacaaatcc agcaatactt gtcaacactc ctcctagaac agaaagtgca
     9901 acatgtattt cttcaccagt ctttggtaag acttttgttt ttccatctgc tgttttgatt
     9961 tcaatattgc cacttggaag tttctttaca ggaatcgaca aatcctttac caaattccct
    10021 tcactatctt ggattaatgg aataccatct tttacaccaa caattgtttg atcacctgct
    10081 acttttaatg gggtggttgg tgttggaata tgggtcaatt cctctacatt actagtgaca
    10141 atcggtacac tcattccttg agaagtattc gtcgtaattt caccgcttgg ggttaccgat
    10201 actgtgactt cttttgctgg aacaataggt ttattttctt ttggaattgc ttctgatttt
    10261 tcaacaggtt gtttcggttc ttctttttgt tcttcactgg tagacgacat cgttggctgt
    10321 tccgtacttg acgaatcagt attcttttca gaagaactgg ttgttcctga ttgttccgga
    10381 gttgtgacta tacttgaatc cgttgttgtt gaactatgca caccttcttc ttcgatcgaa
    10441 tcagtcggta aagttgattc aggaaactct ggttctggtg tcactggctc tttttcaggt
    10501 ttctctgact gcaattgttc aggttctgct ggtttttccg ttgttcctcc atttagctct
    10561 gtctcaacat ctggtaatcc ttcccctggc aatgtttcta caatttctga cttaggtgtt
    10621 tctacagttt catctgcata aaccaatgaa ctccctaatt gaaacgaaaa taatgaaatt
    10681 cctagtgttg ttacgactgc taatgttgtt tttttcatga ttattcctcc aattttttat
    10741 gcaattcgtg ttttgttcat agacgccgca ctaatattat ctgaacgata aaagaactcc
    10801 cgtccgtttt cttctaaccc aacatatgga ttatttttga ataattgaat caatagttca
    10861 ctggtgttgt attttggata gtttgcaaaa aaataatact gtgcttgtcg cccatccgaa
    10921 aattcaacaa gtaattcata acgataccgc ttcaatcctt ttcgttgttt taaaggatct
    10981 ctataccaac ttgtccatcg ttccattcgt ggatagagat agacacgata cacatagaac
    11041 aatgccatac cacataccac caaaaataat aatcgccaca taaaaattcc cccttttctt
    11101 ctacctttct tgaatcatcg aacattcata ccctctatag agatttgaaa aatccatcat
    11161 caattgttca tataaacgtt gttgcaaata gcgattgttt cgaatacata attcttcata
    11221 gccatcaatc acttgatctt ctttttcata gcatcttgta gctctatgac gctcagcttg
    11281 caagacataa ccaaactctg caccatatcg ttctactgtt aataactgtt gactacgcaa
    11341 taatgcaata taacaatcac aaaaatgttc aaccgacatt tgatttttct cacgtttgtg
    11401 aaataggtat tctttagaaa gcacattatt cgttaaacaa tctgataaat agcgataatc
    11461 tggcggtcgg ttattttcat ctgtgaattt ttcccataat aaactgattt ttattcgttc
    11521 agcggctaga tagtcttgtt cgcgtggata aaattcatca atcgcacaaa gttttttatc
    11581 acctgaataa acaaatccat ataatgcgaa acgaactctt ccttttcaat tggctctcct
    11641 aaaaattcca gtgacatacc atcttctcct tcctaaaaaa aataaaaaag cagaaaccga
    11701 tttggcttct acttttcttt ccttttatag ctctttttta cggaagaatc cgcaaatgac
    11761 attttttcaa aaaatcaatc ggcacaagtt cacgaaaact tggagcaatt ttggatagaa
    11821 tcacttcccc atcttgtaaa atacaatcat aaaaatcatc agttacttga tacttacgct
    11881 tcttacaata attgataaaa tagtagcgat cattttctat ttccattcgt tgttctacta
    11941 agcgcatacg aacatcatca tagaaataag ccacaaatcg cccttcaagt tcatgttttt
    12001 caatgggata ctcttctggt aacaatctat tttttcgtcg tttatacatt gctggatacc
    12061 ttagaagaag ttcatctaaa cgtctgtcag tatatggtaa ctgacgaatc aacgattcta
    12121 atcgttcttt atacagatag aataattggc gcaagcaata gtgtaccgcc tctggatttt
    12181 ctaatactgt tggatcacgt gaccaatacg caccaacata aatcataatt tttttcattt
    12241 agacaatcac accagcaata ctaatgaccg atccacaata ggtagaacaa gtggcatagc
    12301 gaccaatttc ctctaattct atctcaatct cttctaaaga aagatatgtc tgttcttctg
    12361 ttggctggcg actcatttcc caagtgacag tcaccaatga aatttcttcc ataagaggtt
    12421 tttcgctgct aagtgctgcc acacagtttt catatatctt cattttatcg cagccttttt
    12481 taaccgctaa aatcgcaggt attggcaatg aaaatcgata aaagcgaatg aattctagcc
    12541 ttcctttcct tcggctaaat tccaactctt cgagttcttg ttcgtatccc ttacgaactt
    12601 ctaataagcg tgcaatttct tgccagttct ctttttcctg ataacgtcca gcttttaccg
    12661 cttgttcaat ataaggaact cgaaaacgaa aataagacaa atcaaccatc ttttcattta
    12721 atgcttcgat tgcagccttt aaatccaaca ggcgcagcca ttctgttgtc gtatatgcat
    12781 tcatgcaatt ttccctcctg acgttcccat ccattctaga tagttttgtt gctcgtcatt
    12841 tagttcggca ttatcaatcc cttcttgtac aaagtctctt cctaaaatac gatctgcttc
    12901 ttgtaacaac tttaatgtcg gagctacttg tttcaaaaac cagcgttctg aacgatctaa
    12961 tgagaatttt tctggcttta ctgtaaaatc caattcttcc atcgaacgaa ataattttgc
    13021 ccatcgttta tcaacaatcc gtgcatcact ttccagtcta tcccaaacaa taagttttgc
    13081 attaattaaa ccaacgccaa tctctcctaa acgtttcggt gaatcaccta aaataaatgc
    13141 ttctactgct tgtgttgctt tctcattggc taaccgtaac tcatatcgat tataaatacc
    13201 atatttcgtt aatgcttcta attctgaaat tttttctcgg ttcgcaattt caaaacgttt
    13261 ctcataaaaa ttaaaaaaga gaggactctt tcgcgatcca aaatacaaag ataatccttc
    13321 acttcgaaaa tagccatcat ctaaaaaaca accgccaata ttctggaatc tcttaaagtc
    13381 catttctaat aaacctgctt tttgtttttc tagtaattca aagagatcga aatgttcttc
    13441 cgtttccaat ccccaaagtt catctaacgc aatatcgaat cggctacatt gcacacgtgt
    13501 ataaccagct tccaactttc cttgattttt ccaaatggaa tatagccatg ttcgccaatc
    13561 tgtttgttgt tcacgcatga cttcctcata ttcccggcaa ccttgtcctg aaaattgcac
    13621 aagtacattt tctgttacag ttcgttttga atgccagaac acccagatat tcccacgaac
    13681 agaaagacag tcaaaaagat acaagcccgt tggacgtgtt tcaaattctg tcagatccat
    13741 tcgtaataca cgttgaatca ctgttttgta gtctgtccca aaaaaggaaa gacgcaaata
    13801 atcaatcatt gctcgaagat ctattccgct tttcatatac aatcgaaagc gttccttgat
    13861 tcgttgttca aaatcctctg ggatttttct tttcccttgt tccatctttc taatgtaatc
    13921 ttttgagcag cccatcaatt gcgcaaatct tgcttgtgat agcggctttt tatctccact
    13981 tatattatga attgcactta acttctttct gtagcttttt agctcaatcg gtgtcatttt
    14041 ctcgctcctt ttataaaata acaaaatcga ttttttttaa ccctttagaa acaccgatat
    14101 atcaactagt ccccctcgtt ttttgggaaa agtagtacaa ctgctgacct tagataatca
    14161 cttgatacaa aagggtttat aaaacttttt tctatgtgtt atgttatttc tgccccccat
    14221 attatatatc ggggggcttt acacaaagag gccgtgcgca agcgcacgac ctctttgtga
    14281 gctgcacacg ctgctagcga gtgcgcatta taggcccatg cttcgcagcc ttaatgcgca
    14341 ccgccaggca gagtgcggct accagactac gtctagagcc ctcccgatag ataataacta
    14401 caaaattaaa tgatctaatt ttgttatttc actatatttt tcaggagaaa aaacgcgata
    14461 aattgacaaa gttaaatcct cttctaaatc tactcgataa tgatacaatc gagtcaatcg
    14521 ttctatatct tttttcgtta gcaatacttg attaataact tttcctgtaa aaacacgttc
    14581 tttgcgcaaa aagcggtaat cagttacttg ttcaatacga ttcaaccaat cataaaacgt
    14641 tctggaagaa agagaaggat taatcattcg tattgctgta gctactctat aataacaact
    14701 ctctgccatt taacttcacc tccttagttg attacaaaat atgcgcatca gaaaaacttg
    14761 ttccttttgg atcttttgct aaatacattt ggcgctctag ttgttcttgt aacaactgat
    14821 tatcatctcc catcgtttga tcttgactat cctcttttac tgtaaaatca tagacaatca
    14881 tttcagtaaa tttctcaaat tctttgataa agtgataatc tttgtaaaca aatggcgcaa
    14941 aaaactctac aggtacatca aatcctggaa aagcatatcc acgaccaggt tgatctttgt
    15001 tgtaaaatgg cttattttta aaatcttctc caaaagtcat taaataacca atgggagaca
    15061 atcgaccaac agaaatacgg aatgttaaat tatcccgaac accacttgga aaatattctg
    15121 catctggtcg ttgcattgct aaaataatgt acatccctgc ttgacgaccc tttaatacta
    15181 atggctgaat caatttatcg actctatttc tcgcttccat atccaacgaa ccataaaaag
    15241 ctgcccattc atcaaaaaag ataaaatgcg gtggtaaccc ataataagca taatttttcc
    15301 ctggttcata tttaggatct ttcatggttt tcatataggc ataccgttga ttcatttctt
    15361 ttaatgcatt tcgtaaacaa ataatcattt cttcatttat tttgtaaaac acatgtcctt
    15421 tgaatacatc gacgtcacct aatgctgcta aatctgccgc ttttccatcg caaatatcta
    15481 ctgtcccaac acttaaacaa gcttgaatta aagaatacaa taaataggtt ttcccaccac
    15541 cgattgcacc agcaatcaac atatttggtg attgatcata tacccaatcc aaagttttca
    15601 ttaaatgaat acgtccattt tctgctatac attgatcaat aggaatccga ccagaatctg
    15661 aaaggaatac ataattgatt cgggcttttt ctcgttcttc acgaattaaa tcacgcaaaa
    15721 atacttcaga taacaaatga cccattttta aaaatcgttc gtgaaaacgc ccaccatcta
    15781 aaggaaaaga aaccactaat tcttgtttct tttgataaaa ataaactttt ggtaatctca
    15841 ttttttccct tgtttgtttc tcactaactt gtttctttgt ataaacaaac cgattgtcaa
    15901 ttaacattcg aacaagtaac tgacgccaat aaacagaggc aaaatatcct tcttttagtc
    15961 gcgaccaatg catcaaccct aaaattaacc cactaaccag caacaaatac aacacaatac
    16021 ttattccagg aataagatac atttgccaat tcgaaaacgt acttaaacgt ggataaaata
    16081 ctttccaact aaaccaacct aacagaatca aaaatggaaa caaaaagaaa ccataatagc
    16141 aaaaccataa ataacgattg aaatagcgaa ttcgccgacc acgatacttg aaaaaatgag
    16201 ctttcaattg tcacattcct cctttctgtt tttacttttc ctgtgctaaa aacttttgac
    16261 aaaaagcaac taatacagga atatcacatt tagtaaaaaa acataatcga aaaagattta
    16321 atcgattttt ataagggtgt gcacacatat tacatattcg tactatacga atccaaatca
    16381 cttcctgatg atcttgtttc cactggtacg gtaatgaaaa acgaacaaga tcacgtaaaa
    16441 acatacactg cccaataacc gcacaaatat atgcatttgt ttgtacaaaa atcgttggat
    16501 tgtctccaaa atactcaatc aattgttgga tattttgata acaagcacaa tctctcaaag
    16561 tatttgattg gttttatctg aattcaaaat ctccccttcc ttctatctta gataaaaaga
    16621 ataggaagac tccttcctat tctttcaaaa aaaccttatt ttttctcttt ttccttagaa
    16681 tcaacacttg gttgtttttg atctaaacct ttttcttgtg attgtttcat attaggatta
    16741 tttgttggag cttctacttt ctctaatcct ttcgcataaa tcactaaatc tgaatattca
    16801 cgattgccat cttgtgctgg aatatatacc acatgatatt ctggatcaat taatttcaca
    16861 ggatcacgtc gtttaaaacc agtcaaatct acatctgctg gaaatttaac tcgtcgtgca
    16921 atctttggtt catctggatt tccaaaaact aattgtgctc gctgtgatcg aatttctccc
    16981 gtataatcgc ctgtttcatt atcaaataca cgatcagact cttctaatgc gccaaagcgt
    17041 acggctccta aacaagtttc atctaatgct tctgtaatgt cataattatt tggaaatttt
    17101 aatcccatct tacaaaacct ccccaatttt tacaccagtt aaaaagacac caacatcttg
    17161 atagccagtt gttcgacgct ctgtttgtcc catatcattt gtttcacgac tattcactcg
    17221 ttctttacga aaaacaaaat aagcttcttc aaatgaaata cgcacctctt tttctggtaa
    17281 tcgtaatctt ttatcctctt cagctgtgag ataaacaaaa aaagcaggct gttcttctcg
    17341 cgaacactct gcttgtaatt gaacatatcg cccagacaca ccattaattt cctttggtgg
    17401 attaggttcc cgtgaaccca ataaaaatac ttgacctaaa aactctttct tcatacgcaa
    17461 tgctttttct gccataaagt ttttcctcct cttaaaaacc aaaataaatt ctaaatattt
    17521 ctacattaca atcaaacaag cggttaaaaa tcctaaccgc ttgttcctca tgactaatct
    17581 ttggctttac gtactttata aagtgttata cctaaagcaa tgactaatac tactaatcca
    17641 agaaccataa gtatactact tgtttgttca cctgtttttg gcaacattgg tacaacagta
    17701 gctggtttaa cccgttgttc tggtttcact ggttgcggtg gttttggcgc ttccacaatt
    17761 tgaaccgttt gatctttgtt tgttagatct acattgtgtt tcgccacttc ttcaaacgtt
    17821 tctttctcat cttccgtttt cgcttggagc cattccgcaa aactcaagcc ttttcctgca
    17881 agattgcgac cgtccactaa gaattcaaat aagaattcca tcatttcttt atcggccgca
    17941 aagacttttt ccgcatgagt gccttcaaat ggtgtgccgt ctggtgttaa gtagccttga
    18001 acaattaagc gatagatttg accaatcgca agaccgtcta ctttgaccca ttcaagcaca
    18061 gtttgatttg gcaaggccaa gatctctttt ccgccatttt tattcgtcgc tttcgtcgta
    18121 atggttggat tactgatttt gatggtttgg tcattgtcgg taatatctgc gtgagaagcc
    18181 aataatacgc cttctctaaa cagatcttca aagaccacga cttctttgcc tcgcaacgca
    18241 cgtgcgtcta gaataaagtc tagattgact gtaccatccg ttgtttctgc cacaaacgtt
    18301 aaggtacttg tgacttcttt tccatctact agcactggtt tttccgttac tttgtccatt
    18361 agaacaccgt tgactgtgta tgtttttcca acgaccaaat cgtggtattt cacagcgtct
    18421 gtaatggtga ctttcgttaa tggattaacc tctttcaagc cagtgggttt atgtgtagcc
    18481 gttgtacgta cacttgggtt agttactcgt accgtttgcc ctttgtcgtt gatgtcttta
    18541 tggtttgcta gttcttcatc atttctaaaa agactttcaa acacgacgat ttcacgacca
    18601 tttatacctg tcaaatcaaa ggtaaatggt acgatcaccg tttcattttc tgctttggca
    18661 ataaagccaa cactcgtttc taccgtattt ccatgcacaa gcaagggttc accggttgct
    18721 ttatccatta agatcccttt gaccaagtat gcttttcctg gaagaatctc ggtaatttca
    18781 acggtatcgt ttaaggtaaa caatcctgtt gggtctgcga ctgtcaatcc gtcttcaaag
    18841 acagccgtgg tatgaacggt tggattattc actttcacac tttgggcttc atcgttgata
    18901 tccgcatgtg ctgcaacttc aatgccatca cggaaaagtt tttcaaaaac aacgatcgtt
    18961 tgacctttca gaacactagc attaaccaca aattcaaggt cttttgtgcc attgactttg
    19021 tctgcaataa aggccaaggt acttgtgatt tctttgccat cttgtaaaat tggcgcattc
    19081 gttgccttgt tcatcaatac tcctgaaatg gtgtattctt ttccgacaaa caagttggta
    19141 taagaaacac tgtctttaat gacgatcgtt tctaatggat caaccacttt ttcaccatcg
    19201 gctttatttg acgcttgtgt ttctagtttt ggtggaatca agtaattacg tacagaaacc
    19261 actaacgttt ccccatcatc tggcgcattt tgtttgaatg caaccgtgaa tgcttgttta
    19321 tctgtatttg gttggaaact ttctaatggg gcattttcca agaagtaata gtcatcggca
    19381 actagacctt tcactaaaat ttccccgttt tgatcagtgg tatattcacc aattgcttga
    19441 tttttaccgt tatacaaggt aaagttcaca cctgcgagcg gcgttttctt cttgttccat
    19501 tcttcatact tgaataattt tacacttttc cgtgcttgac ggttcgcaaa atggatcact
    19561 ttgatcggtt ctgtatcatt ggtaatcgag aacactaatg ggttttgatt caaaacgtaa
    19621 tttacggggg ctttgatttc ttccaaacga tagcgatctt ttccataagt aagtgtaccg
    19681 ttagttagga aaaagccttt ttcattggtt ttaaagatat ccgttgtttc tgtatcattt
    19741 ggaatctcca ttgtgacata acgattggct aaagtatcaa aaatcttgaa ttctgtatta
    19801 gcaagtggaa tcacttttcc tgtttcttga tccactttca ccactttaag acgttgttca
    19861 attactttat cttctaaaac ataatggaaa gtttgcccat tttcagaaat tgtgacttca
    19921 agtggttcaa atagtttata cccttctttt cctttggttt cagaaagaat gtacgtatca
    19981 aagacaacgg tatttttacc aaaagtagca taaccatcat tgtctgtcac aacggttttc
    20041 accactttgc cagttgtttt tgacgtcaaa gtgatttcaa tgccagctaa ttttggtttg
    20101 ttatctggat tcgttgaatc gactaaatct ttattagcaa ctttcactaa atcaatggtt
    20161 cctttgatca cttgatcttt tgcggtcgca ttgacagttt ttccaggttc aacaacaatt
    20221 tcaattgctt catcagaaag cacataacct tttggcgctt tgatttcttt taaaacatat
    20281 ttgccaggat caagatcttt aatttctgcc tgtactttgc cgttaatttt tttcaaggta
    20341 atttctttaa tctttttccc atctgcttga tacaaaccat agaccgcatt ttctaaagaa
    20401 gctgcacctt gtgctttcac accagtttcc gcatcttctt taaacacacg aatgtttcct
    20461 tgttttagaa tttgtaaatt aataacagac gcattaggtt ctgttggatg taacacacca
    20521 attgtttgag ctcctggacg acgataggct aatggcgcac cttgaacttt aattttattc
    20581 aattgaattt ttgctttatc cggtgcctct tttgtgactg taatccatac ttcattgccg
    20641 tgaatagata cattgacacc ttttggtttt tgaatgtctt tcacatattt caaaaaatca
    20701 tttttattct ttactttcaa cgtttcgcca atttttaccg tatgtgtttg attattaaaa
    20761 gttgcttttt caccaaagtt attaatcaaa cgttgaattt ctgcttttcg acgatcataa
    20821 tcaacacgga tatttttgat tgggattttt gtgcccaaag cttcccaaat cataaattgt
    20881 gccgcaatgt attgttcatc ggatttgtct ttatttgtga catatcctaa ataagagatc
    20941 aagcttgttt ttttccgtac atttgaattt ttcaaatagt ttttcaaagc agtagctgta
    21001 taaacttgac cttctgcggc tgagacacca ggctcaatac aaaacgccac ttgtccatta
    21061 atctcaaaac gaaccatata atttaaagtg aagtttagat tgttcggact agaagtaaca
    21121 ccaatatttt cgccccaatc aattttccga ccacctaaaa cagctgcatt ggttgcacgg
    21181 cgaacacgaa ccgaacgatt ggttggtact gttactaaca gtgtaggtgt acctgttttt
    21241 ggaatgttct gctctttttt cttttgattt tcaatactag ccgtagattc tttgtttggt
    21301 ttcgttggtt gattttgact agctgcggtt gaagagattg ttttttcaac gttttcctca
    21361 ttttttcctt ctttgtcagc agtagccgct ggttgttcca ctgtttcttc tattcgtttc
    21421 ttttcttccg cttgtttctc agaagactct ggctgcacag aaggcgttgc ttcggcagct
    21481 ttttctcctt ctagagtgga cgtttctgaa gcaggggttg taattgaggc agtttcttca
    21541 actggttgtg aagtaccagg actattccct gggtgttgtg tttccccaat cgtctgatcg
    21601 agagtcgttt tggaattgac cgttggcgct tctgtcacag gggcctgttc aacagtggct
    21661 tcaccagctg ggaccccggc ttctgctggt tggacttctg tcacttcaga agcgacttgg
    21721 gcaatggctt ccatcgtagg ttgtatagcc gtcgtttctt ctgcatgtgc ttgagcagtt
    21781 cccaccaaga aacagaattg agaaaaagcc accaatgcga gtgctttcaa ccaattcatt
    21841 ttcgtttttg catacacaag aattcctcct tttcctgaga attttatata ataaaacaaa
    21901 acgccgttac caaaagtgat aacgacgtga actgtctctt ctatttttat ctggcgctct
    21961 tatctgaaca catttcatca atgattttac cctcccacta taattacaga acaaaaaaag
    22021 aacctgcttc gtggaaatca actgtccatt tcccaattgg aataccagct tttttggcaa
    22081 cttcaccatc accaaatctc atgccatacc aattactacc ttcatcctta atttctttcc
    22141 aaccatatga catagcttca tctaatgaat caaaaatcat gacactattg ccaattgtta
    22201 caggatctgg cttaagatct tctggaagag aaatatcaat cataccataa tgatccgatt
    22261 cctcagtatt attgtcatta ctagctactg gtttggttgt tctactggct tattttgata
    22321 caacaagtac tcttattctt cttttacttc tccgttttca atttcccatt caaaccactc
    22381 cgcaaagtct tgcttaaaac catctactcg atacctattt tgtaaatagg catcttctga
    22441 cgtcgtagga tctaaatagg taggtttatt ctctggacga gcatagacac ctacccatgt
    22501 ttcgtaatct ccttcaaaat aactatctac caaatagcct ttttttctaa gaatctcccg
    22561 aacttgattt ttaattattg atacttgttc aaaaccatca ctcattctat cacctcaaat
    22621 aactctagat tctcataaat attcccgaca atttcaatcc tatcaatgat ctctaacaaa
    22681 tcgctactta tgcttcccca ttcgtataag aatttccctt ccgcaaaata aattttccat
    22741 aaacttcttc atgatcgtcc caaccaatat cactctcgaa aaattccacg ttgttcttat
    22801 cttttagctc agtaaatgcc ataagttcaa catgttttcc aacctcgaaa caatcacacc
    22861 acccatcaac cgttacacgt gtgccttcaa tacctaagaa actttgtcct cgtgtattaa
    22921 acactaaaac tttccgcatt ttttgctcat taattaatca cgcgcgaaat ctaggaatca
    22981 caacttattc gcttcttctt tataaataaa aaaactccaa taatcggaaa ttactatctt
    23041 ttcctcattc cttttttaaa tcttcatact tcttcttatc tttgtcctaa cctaataaca
    23101 caaattttga agaaaaactc agtaaaacct gattcttttt ttatcttcac aacaaccaac
    23161 tttttcttca gatcctttta ctagaacttg tatacaaaaa ttgttgaatg ctgatataat
    23221 ttgcggctac ttgaaaaaac gatagatgta aaaagataaa tagaatcatt ttttattcta
    23281 tatcagtcta tgattcattg cttatcaatc acccaaccca ttcgatgccg ttttttttag
    23341 agaaatttaa aaaagaccgt acgaatctcg cattatccga ttacttttcc agaaaagatc
    23401 cccaattctg tataaataac cctatcttct tgttggtttc attttctcat ataagaattc
    23461 agtcaatcta cccactatga ccattactac gtccaacaca actgacatat taaccacgta
    23521 agtattccct cacagatctc cgaatctcca tggtatggat tcccatcctg acgctgcaga
    23581 ggacaacata tttcatgtgg ctctgtaccc ttttgatttg gaaacactac aaatcaatac
    23641 tcatctataa cttatcaagc gaactttcgc taggcttcta gtgattacta aattgttgga
    23701 taacatacat agcccatttt atctagctgt tattcataac agttagttct tgtattactt
    23761 cttccgcttg tgttgccatt gagcttgtca aaaagttact tagaaaaaag ctgccagtaa
    23821 agcaactttc aacaaaccct ttttgtttct tatcatatca agaaacctcc ttttataaat
    23881 tttttctata ataaaaaacg tcgataactt aataagtatc gacgtaaact atttttatat
    23941 cccgtaaatc aaaaattcta aagaaagatt tcctatataa gcatattgga taattcgatt
    24001 agtattgggt atatctctac cattctcata cctatctatg gaccattttg aaattgaagg
    24061 agaaaaaagc tttccaaact cctccctttc tagataataa tctttcctaa ttttcctcat
    24121 ccttaatccc atactaaaag aatttatctt tttaaaatcg ctaactcttt catcttttgg
    24181 aagtatattc ttataatctt ctccgtacat aaggtaagga acagtgacct taccaagata
    24241 ggcaatattc attagttttt caatatctgg aatattaata cctttttccc atctacttat
    24301 aacatttttg cctgtataga tggtaaagtt tttaccaaat ttttcaagcg tctcttcccg
    24361 gttctttcgg acttctcgta ttctttttcc cataacaatt ttatcgtctt ttgataaact
    24421 aaatatctca ctatcacgat taatctcctt caaaatttct ttttcattac ttaatagaaa
    24481 aatatcgatt ttttttgcat ttcttttgac ggatttctct ttctatttaa cacataactg
    24541 agtaatactt cactaattcc tatattatca gctaactctt tgttaagtaa cttattcata
    24601 tctctgtatg caattagttt accgatgaga atctcaatct tatcttcctc atcttttatt
    24661 agagtttcta tattcagaat cttgcttaga gtattatcta cgtctttagg gatttttctt
    24721 gacccttttt catatttaac taaagtactt tgtgaaatac ctgctaaatc agctaacttt
    24781 ttttgagata gttttttctc ttttcttaga gatttaagaa cccttccact tgaattatca
    24841 accatgaaaa aagcacctcc tatgagatgc tttcatcata tactgacagc taaaaatctt
    24901 caagaaaaat attccaattt tgttaatttt agtatcgccg tgataaccaa atgacagttt
    24961 aaaatctttt atacagataa tcctcagtgt tgcatctact caaaaaggtt tatttaatag
    25021 gtttttcgat tgattataag ttatttcaca ctatcttgct catccatttt cagaacggcc
    25081 atgaaggctt cttgcggaac ttcgacggag ccgatttgtt tcatccgctt cttcccttct
    25141 ttttgtttct ctaagagttt acgtttacga gaaacatctc caccataaca tttagccaag
    25201 acgtttttac gcaaggcttt gatgtctgaa cgggcaacga ttttttgtcc aatagctgct
    25261 tggattggca cctcaaattg ttggcgtggg attagttttt tcagtttttc aacgattgct
    25321 tttccacgtt cataggcaaa gtctcggtga acgataaagc ttaatgcatc gactttttct
    25381 ccattcagca agatgtccat cttcactaac ttgctttttt gataaccaga catttcataa
    25441 tccaatgagg catagccttt cgtgcttgat ttcaatttgt cgaaaaagtc aaagacaatt
    25501 tcagaaagcg gaatattata gacgacattt actcggtaat catctaaata atccatcgta
    25561 atgaattctc cccgttttcg ttgagaaagt tccattactg caccgacaaa atcatttggg
    25621 accatgatct gtgctttgac gaatggttct tctacatcct gaatcgttac aggttcagga
    25681 aaatctgccg ggttatctac tgtagcagtc gtaccatccg ttttattaac atggtaaata
    25741 acggatggtg cagtcgtgat caattctaga ttgaattccc gttccaagcg ttcttggacg
    25801 acatccatat gaagaagtcc gaggaatcca cagcggaacc caaaacctaa tgcttgagag
    25861 gtttctggtt cgaattgtaa agcagcatca ttcaattgca gtttttctaa agcttcccgc
    25921 aaatcgttgt aacgagaagt atcaattgga taaagaccac aataaaccat cggattcatt
    25981 tttcggtaac ctggtaaagc ttcagcggca ggattgtctg ctaacgttac tgtatcccct
    26041 actcgtgtat cttggacagt tttgatactt gctgtgatat aaccgacatc accgaccatc
    26101 aaaaagtctc tagcaactgc ttttggcgag aagaccccga cctctgtaac atcaaatgtt
    26161 ttgccattac tcatcagttt gattttgtct ccgggtttga ccataccgtc cgtgatccgg
    26221 acatttagaa caacaccgcg gtaactatca taaatagagt caaagatcaa agcttttagc
    26281 ggtgcatcta agtctccact tggtgcagga acatattcca cgatctgctc gagaatatct
    26341 tcaatcccaa tccctgattt ggcactggct aatacagctt cactagcatc gattccgatt
    26401 acgtcttcga tttcttgacg gacacgttcg ggatctgctg cgggtaggtc gattttattg
    26461 atgactggca ggatctccaa atcattgtct aacgctaaat aaacattggc caatgtttgt
    26521 gcttcgattc cttgtgctgc atcgaccact aaaacggcac cctcacaggc agcaaggctt
    26581 cgagagactt cataggtgaa gtcgacgtgc cctggggtgt caatcaagtg aaagatatat
    26641 gtttctccat ttttcgctgt gtaattgagt tctacagcat ttaatttaat cgtgattcca
    26701 cgttcacgtt ccaaatccat cgagtctaac agctgatctt gcatttcgcg agacgtcaca
    26761 gtgtgcgtca tttctaaaat acgatcggcc aatgtagatt tcccatggtc gatatgagcg
    26821 atgatcgaaa aattacgaat ctgctcttgg cgttctttca ttttctttat atccatagcg
    26881 tctcctcttt tcatataatc agcactttcc attataccaa cgaaaagcag taatttaaag
    26941 agtttttaat tagtcagcta tagaagtttt ttttgtagaa ttttgctata ctgttatcaa
    27001 gctaataaat ggagatgaaa aaatggatca aaaagatttt agagaaaatc ttgaattaaa
    27061 accggtcact caagaacata tcgatcaatt caatgaattg ctttcctatg tgttccaagt
    27121 gactgaagca gacatcgagg aaagcgggtt tgagaataaa cgagctttta tccgttccaa
    27181 aaaaccgatt ttggaagtat cgaaagtgtt cggatggttc cacgaaaata aactgatctc
    27241 tcaaatcgct atctatcctt gtgaagtcaa tattcacggt gcacgttata aaatgggtgg
    27301 tgtgactgga gttggaactt atccagaata tgctaaccat ggattgatgc aagatctaat
    27361 tatcgttgca ttggataata tgcgcaagaa taagcaatgg atctcttatt tgtatcctta
    27421 cagtatcccc tactatcggc gtaaaggatg ggaaatcatg tctgataaac tttcctttaa
    27481 aatcagagat acacagctac ctaaacaaat cccagtaagt ggtatggttg agcgattacc
    27541 agttgatcat ccagacgtat tttccgttta tgacaaattt gcccaacaaa accatggtgc
    27601 tttattccgc agtgattttc attgggaaga gtattggcgt ttcgaaaatg aagatgagcg
    27661 cacagccgct gtctactacg atagcaacca tgaaccaaga ggtgttctgt tctactgggt
    27721 agctgaagaa atatttcacg taaaagaaat gttctatctt gatcaagaag ctcgaaatgg
    27781 cttatggaac tttatttccg cacatttttc catgatctac tgggtacatg gagatattta
    27841 taaaaatgag cctttagcat ttttgattga agatagtcag atcaaagagc agatcgaacc
    27901 atattttatg gctcggatcg ttgatgtaaa agaattcctt caacggtttc cgtttgttgg
    27961 aacagctgat gcttttcatt tcatcattga agatcctgtc gctccttgga ataatggtgt
    28021 cttcgcttta acatgggatg aacagggaca agtccgtgtc ttaaatgaac ctataggaaa
    28081 atccgttcga ctggatatcc aaacattgac ttgcttgatg atgaattatc gccgtgcttc
    28141 ttatttggca agaatcgaac ggttagaaac agatgaagaa acactgaagt cactagagcg
    28201 tatcattcca aacatggaag cttactttag cgattatttt tgataaaaag gatgatgttt
    28261 agatgaaaat ttattttgcc gcaccaatgt ttgctaaaag tgatttgtta tataaccaac
    28321 agttagttaa agaaatcaga gagctctctt ctgagctttc gatctatctt cctcaagaaa
    28381 atgaagcaat caatgacaaa actgcttatg cagacagtcg aatgattgct cttgctgaca
    28441 cagaaaaagt gttggctagt gatttaatga ttgctttact agatgggctg acgatcgatg
    28501 ccggggtagc atcggaaatc ggtgtggctt atgcgaaagg catcccagtg atcggcttat
    28561 atactgatac tcgacagcaa ggcggaaccc acccgaaaaa aatcgctgca ctacaagaaa
    28621 cagctgaaaa tcaatttcac tacctgaact tatatacaat aggtttgatc aaattgaacg
    28681 gaaaagtggt ttcttcagaa attgagttgc tttcagaagt aaaaagattt ttagatggag
    28741 ggactttcag tgattaaaga aatcaaaaag gtgcaaatag ctttacttgc ttttggtgta
    28801 ttcgttgtct gctataattt ttatgaattt atcacacaaa aatattcgac ttctcaagga
    28861 atcacattta tcgtggaatc tttattaggg attgcattga ttttcatgcc acaagtcatt
    28921 ttgaaagttt tcaaacttaa aataccagca gcaatcgttt tgttttactg gtttttcttg
    28981 ttcatctctg tctttttagg cacagggatg catttgatca gtatcatctc tttttgggac
    29041 aaaatcttac atgcggtgag tccaatggtc ctgacagctc ttggctatgg attgattggt
    29101 tacttgatga aagatgctga gatatcaaaa accagtcctt ggttgttcct cttattcggc
    29161 tttgcgtttg ctggactttg cggcgtgttt tgggagttct gggaattctt atgcgaccag
    29221 ttcttaggca tgaatcttca acgttttgct gcttctgatg gcacactgtt tgtcggacga
    29281 gcagcattga tggatacgat gggcgacttg ctgacaaata cgataggtgc tgccttaatg
    29341 ggattgtttg catggtctca aagtaaaaaa gatgagcgtt actttgaaag ttataaatta
    29401 gaaaaagtga aaaataccta ataaaagagg ttgtgacaga agcgggcagc ttcaagaaat
    29461 ttcggcttat ttttcgaaga aactacttct ggaacactgt ttatttgaaa aaaggaggtc
    29521 tgacactagt tgtgtcagac cccttttttc ttttatagtc tcaatggaag attagacgat
    29581 ttcttctagc tcttctgttt tgaaatgaag ttgtccatca agtaaagtaa tcgttacttt
    29641 tgatttaggc ataacatgtc ctgcaacgat ttctttagct agtggtgttt ctacttcctt
    29701 agtgataaat cgtttcaatg gtcttgcacc atatgctggt tcataagcat tttcagcaat
    29761 ccacgttttg gcttcatcac tgattgtcag caggatttct tgatgttcca gacgttgtgc
    29821 caattgcgcc accattttgt caacgattcc tttcacgttg tctaggctca atggtgtaaa
    29881 taaaatcgtg tcgtcgattc ggttcaagaa ttctggtttg aagtttccac gtaatagcgt
    29941 gttgacttgt tcagccactg cttctggaat cgttccatca gcagtcacgc cttccagcag
    30001 taactgtgaa ccgatattgc tggtcatgat cagaaccgta tttttgaaat ctactactcg
    30061 cccttttgaa tcagtcaagc gtccgtcatc taggacttgt aacaagatat tgaagacatc
    30121 gggatgcgct ttttcgattt catccaacaa gacgattgta taaggatttc gtctgacagc
    30181 ttccgttaat tgaccacctt cttcgtaacc tacatagcct ggaggagctc cgactagacg
    30241 agacacagca tgtttttcca tatattcact catatcgatc cgtaccatat gatcttctga
    30301 gtcaaacaag ttctcagcta acgctttcgc taattcggtt tttccgacac cagttggtcc
    30361 taggaaaagg aaagaaccta gaggacgatt tgggtcttgc aatccagccc gtgaacgaat
    30421 taccgcgtca ctgaccgcat caactgcttc atcttgacca atcactcgtt tatgaagtgt
    30481 ttcattcaat ttaattagtt tttctcgttc accttctaca agtttcgtta cggggatacc
    30541 cgttaatctg ccaacgactt gagcaatctc attttcggta acggactctt gaaccatctt
    30601 aatctcgctg tcttttgctt tagcttctaa ttccttcaat tctttttcta attgaggtat
    30661 cgtaccatga cgcaatacag cagcgcgttc taaatcatag ttattttctg catcttctaa
    30721 ttcgtgtttt gctttatcga tttcagcacg ttttgctgaa acagaattga cttcttcttt
    30781 ttctgtttcc cactgcatct tcatcgcatt tgcttcttca cgaagttcag ctaattcttc
    30841 ttgcaaattt ttcaagcgtt ttttacttgc atcatctgat tcttttttca gtgcagcttc
    30901 ttcgatttcc aactgcatca aacgtctagt aacttgatct aattccgttg gcatagagtt
    30961 catttctacc cgaatcgttg cactggcttc atcgatcaaa tcgattgctt tgtccggtaa
    31021 gaaccgatca gtaatataac ggtcagataa agtagctgct gctactaatg cgttgtcatg
    31081 gatgttaaca ccatggtgga tctcaaaacg ttcttttaat ccacgcagaa tactgatcgt
    31141 atcttccact gttggttctt tgactaatac cttttgaaag cgacgttcca acgctttgtc
    31201 tttttccata tattgacggt actcatctaa agttgtggca ccgatcaaat gtaattcacc
    31261 gcgagcaagc attggtttca acagatttcc ggcatccata cttccttctg tttttcctgc
    31321 ccctacgata ttgtggattt catcgatgaa caagaggata cgtccatcac tttttttgac
    31381 ttctttcaag actgctttta gtctttcttc aaattcacca cggaattttg ctccagcgat
    31441 caatgcaccc atatctaatg aaaaaatcgt tttatctttt aaattttcag gcacatcttt
    31501 gcgtacgata cgttgagcaa gcccttcaac gattgccgtt ttccctacac cgggttctcc
    31561 tatcagaaca ggattatttt ttgtttttcg tgaaagaata cggatgacat cacggatttc
    31621 ttcatcacga ccgatgattg gatccatttt tccgcttttg acttgctgta ctaaatcaac
    31681 gccgtatttt tctaatgctt tgtattgttc ttcttggttt tgtgaggtca ctctttctcc
    31741 tcctctcatc tcttcgatgt ttttacgcaa ttctttttca gataatccgt ttttattcaa
    31801 ataaacagtt aacggataat tttttaattt catcaacgct aaaataacga cttccgttga
    31861 aagaaactcg tcgccgaagc tttcacgtat ttggtctgct tcattcaata atcgaaagaa
    31921 attctggctg aggttttgtc catattggac gtttccacca gaaacaactg gatactcatc
    31981 cagtagacga tcaatttcat gttcaaaaga ttcgacatcc aatccggcat ccgtatagaa
    32041 atttcgacca aaatgattag attgtaaaaa gattttccat acatgggcaa tgtcgatatc
    32101 ttgatgtttt cgagtgaccg caatctgttg agcttcagca atcgcttctt gaagcgtcgt
    32161 agtcattttc tcaatattca tacaggtata ccctcctaat taaaggtaag atcacttatt
    32221 tctttgatct ttacaaaaac tattatatcg ctttggtcaa ttttggtcaa aatatacaca
    32281 tagttttttg actttttttc tcgacttata taaatcttaa aaaaacaaaa ttgattgtat
    32341 gtatataaaa aaagagccaa acaaacgtaa aattatctta tccataacgt ttttctttga
    32401 tttgataaaa agggtagatc ttataaaaga cctacccttg attttcttta ttctcttttt
    32461 ttacttctat tatttcttct ttgatcactc ttcgccgttc agcaatcaca tacacaaatc
    32521 catagagaaa tggcaagata aaggaactga aacgatacag taaaagcgaa gaaactgcgt
    32581 caactgttcc aacaactggt ctgaataata acaaatagac aaattcaaag gaaccaatac
    32641 cagcaggcgt aggaataacc cctgaaagca ctactgcaaa gctgataaac gaaatggtca
    32701 gtagaaaatc tattttagga tggttttcaa tcagaatcaa ataaggaagt acataccaaa
    32761 atacaagttt taacatattc catagataaa tccgcaatag cgcagtcctg tcttggataa
    32821 tggtttggac cgtttccctt aaagagtaga tctgctgatt gcatgtatcc acccatttcc
    32881 gcaaacgctc acttttaaat aacttattcg ataagctgac aaaaaatatt tgaacattca
    32941 agctgacgga catcgccaat aaaaaggcaa caatcacgat cgtcaaaagg ataccagcga
    33001 tgataaacca gatcattgat ggaccattag aataaaaaag agagaattgg atgaccaagc
    33061 cagctaatgc ataagtgatc actgccagtt tgtacataat catatggagt gctgtcaccc
    33121 ctgctccctg ggatagcttc attccttttt tgttataaaa ataaatctcc gagatcagcg
    33181 ttcctgtacc aaacgaaacg attcggtaaa acgcaatata gctagaggtc aagaacccat
    33241 ctttcgttgt aaaattcggc gcgaaaggtt cagcaatttc tttgattgag cgcccttcag
    33301 cgatttggta aactgtccct aatataacag ccgctgccaa aacaacaaca ctggtagaca
    33361 gcaattcggc aaagatatct gcaaatgatt gattgattaa ataataaata atggcaaaaa
    33421 tcacaatcag caaaagcaga ttcagaatga ttttagtttt cgttccttta gacgacatct
    33481 cctgctcctt aatggaaaaa ataaatgaga atcaaatcaa caagtacacc tgcagcgaat
    33541 cctatagtaa acttcttttt ccgagtttta tgatggaaaa cttttcgaga gatcaatcct
    33601 cccaaaccac cgcctaacag gctgacaaat atcaaattat tctcaggaac acgccagccg
    33661 ctagttctag cctgatgttt atcatacccc atcagacaaa ataagtaaaa attcacaatg
    33721 aaaaggtaaa accacaaagg attgtgtaac aatacatcca ttactcttct tccaactttc
    33781 tgttaactac tatttctaag ctttttcttc tttctttggt gaataaactg ttaatccttt
    33841 tgatacaaca gataattcaa ctggaagatc atctgtcgtg tctccatccg ttcgagtgcc
    33901 cacagatttt tcctgcgctt ggattttcat gcgtttggct gtgaaataag taatgccggg
    33961 gatcgtatag aatttgcctc tcatgattct aggcaaataa aaaataaatc ggaaaatatc
    34021 taaggaaggt acgatcgtta catgaaattt tccatcatct agggtcgctg agtcgtcaaa
    34081 atttgtaaaa ccgcccacgg agttagacat cgtgactgtc aacagttggg attttccttt
    34141 ccaatgctcc tcgtctgttt ctaaatccaa atgatatttt ttcttcttca gcaataactt
    34201 aaaaaacttc ttgataaaga tccacgagcc gtaacgctgt ttttcttctt gactgatcca
    34261 aactgctgta tctgctaaga ttccgattgt cagtgtagag ataatgactt catcattgac
    34321 ttgaccatag tctattttcc gtacattgcc ttccaggatc acgtcagcag cttcttcttt
    34381 tttgagggga atctcaagaa cacgtgcaag gttgttgaca gttccacctg gaagtaatcc
    34441 gacatggtat tgagataatg ttttttggaa cgcttggacg atatgatgaa tcgtgccgtc
    34501 tccacctata attactaaag tatcagcttc tatggcttct gctttcttga ctaattccgc
    34561 atcatcaatc gatgggccag tttcagctag ttctacatat aattctggct gcttttcttt
    34621 tacataatct tgaaaccagc gtgctaattt ctcaccctca tctttccctg aacttttgtt
    34681 ataaaaaact agcagctttt ccattcttta cctcctattt actcttcaaa aaagagggcg
    34741 ggacaaccaa ttccattgtc ctgcccttta gccttagaca gatgacattc catcattggg
    34801 acgaacgcct tgtcataagc tgttcttttt cgattttaac tctttgtgtt caattctgca
    34861 atttttacaa tcacgtctgt tgctttttcc attacttgta aagaaacaaa ttcgaatcgt
    34921 ccgtgcatat tttctcctcc ggcaaataga tttggcgtcg gaatacccat gtaagagatt
    34981 tttgaaccat ctgttcctcc acgaactggt tcgatcactg gagagatacc aagctcgatc
    35041 attgcttgtt tcgctaattc cacgatgctc atatcttttt caatgatttc tttcatgttg
    35101 taatattgat catacatatc tactttgatt cgttcttgat caaatcgctg attcaactct
    35161 tcttggatat gtgtgatttg tgctttgcgt gcttcgaatt tttcacgatc atgatcgcga
    35221 atgatataac tcatagaagc ttcttcagga ttcccatcca atgccatcaa atggaagaaa
    35281 ccttcgtatc cttccgtttt ttctggaact tcatcggctg gaagctgatt atgaaaatcg
    35341 attgctaact gtagagcatt gatcattgta tcttttgccg tccctggatg tacatttttc
    35401 ccttggatcg taatatttgc ttgagcagca ctaaacgttt catactgcaa ctcacctaaa
    35461 ggaccaccat cgactgtata agcaaaatct acatcgaatt gctcaacatc gaatttatcc
    35521 gctccgacac cgatttcttc atcaggacca aaagccactt tgatggtacc gtgagggata
    35581 gaaggattat tgatcaagat ctccattgca gtcatgattt ccgcaatccc tgatttgtca
    35641 tctgctccca gtaatgtact gccatctgtt gtgatcaatg tgtggccttc ataatttttc
    35701 aaatttggaa aatcttttgt attcaatgta tagcgttctt ccgcatctag tacgatcgta
    35761 gactcgccat catagttttc aatgatttgt ggattgactc ctactgcatt aaaatctgct
    35821 gtatccatgt gggaaataaa tccaatggaa cgcacttcat gatcaacatt gcttggtaat
    35881 gttcccataa cgaatccgtt atcttcatta tagaaaacat cagctaagcc taactcttct
    35941 aattcttttt tcaattcttg cgcaaaagca acttgtgtct gagtcgatgg agtcgttttg
    36001 cttgttgcat cggagcgtgt ttctgttttg acatatcgta aaaaacgtgg caaaagattt
    36061 tcgtacatag tattctctcc ttttaattaa attgaaatgg gtttgtattt gtttcagaaa
    36121 cgaaaaagtt aatatcccaa ttttcttctt gtttccaaga ttcaaatttc tcgataaatc
    36181 gttgcttgca taacgattca atataatgac cgggatcaat tgcaatcaac ccagcgcttt
    36241 gcatatcatg agccgtatga taatagatat ctcccgtgat atagacatct gctctttggg
    36301 cgattgcttg cggataaaat ttttcaccgc ttccaccgca aatcgcaatg cgcttcacag
    36361 aggattttgc attttttggc tggactattc tcagtccatc tagttggaac gcttctttta
    36421 cttgttctac aaatgcctct atagaaattt cttgcggcaa ttcacctact cgtcctaacc
    36481 caaacatttc gactggttca tctatagcga aaagatcata agcaggttcc tcataaggat
    36541 gagctgaccg catggcttga atcacttgtt tttcgatagt ttccggcaaa ataacctcaa
    36601 cttttgcctc ttgaacttgt tctgtcttcc ctactttccc aatcgctggt tgtgcaccag
    36661 cttccggagt aaatcgaccg tgtccaatcg aggtaaaact tgtaccggta taatcacctt
    36721 gtgtaccagc accagctgca gctaatactt cacgcatttt ctgtgcatga tccacaggta
    36781 cataaacggc cagtttttta taatgaatct catgagtctt tactaaataa ctctccactt
    36841 caataccgag aagttcacaa aaccagtcat tcagtccatc ccaaataata tccatgttcg
    36901 tgtgtgctgc atagacggca atatcatgtt tcaacaaatc tgcatacatt ttttcttgtg
    36961 gctgatcagt aaccagccgt tttacaggac gaaagattgg aggatgttta gcaatcagca
    37021 aatcgatttt tttcttgatt gcttcttcga ccacttctgg acgaacgtct aatgtcatca
    37081 ttactcgttg gattggctta tccaatgttc cgatatgcag tccgactggg tctccttctt
    37141 cagctagcca ctgaggacaa tagctttcaa acttttggat gaaagtgcgt ccgctgattg
    37201 tcatggcttt aaaacctcct caatcatttg gatttcctgc tcgatttgtt tttgacgtgc
    37261 ctcttgatta gctgcttgtt tgagctgttc acggatagct tttctttgtt gaagctctct
    37321 tgtccatttt gatttgaaga tctcactttt ctcttcaagc agaaatggcc cgaacatcag
    37381 ctccatttca ttgtaagtaa ctggtttttg ttctttttcg gctacaatga tttcatatat
    37441 ttttttattt tcttcaagta ttttttctga ttggatttta tactgatttt cttgcagcca
    37501 agatcgtaat gtacgctctc cgatgtttgg ctgaaggatc aaacgttctt gtccgcttaa
    37561 gcgttgattc aacttgccag cttctaggat atctcggatc agtgaacctc ccatacccgc
    37621 aatcgtgaca gctgtaatac catcttgtgg ctggatagca tctagaccat ttgcaaggcg
    37681 tacggtgata cggtcttcta atccatcttt tttgacttgc ttacgtgctg attcataagg
    37741 accttgaacg acttcaccag caactgcata ttcgattttt cctcttaata tcaatgcaac
    37801 gggtaagtaa gcgtgatctg atccaatgtc tgctaaacga gcggattcag ggacaaagtc
    37861 acccactgcg gctaagcgct gagataaatc taaatggttc atagcttcac tccttctaga
    37921 taattatagc attttccaaa taaaataagg agactaggaa taaaaaaatt cccatatctc
    37981 cttaaaaaat ataccttttc gttctataaa acaacatttt cttatttttg aaaaaatgtt
    38041 attggttatt tgttcaatga aacatcaata atcgaatctc caggaacttg cggtgggata
    38101 gacatcgtca tgatcggcat ctcaactaat gtaaattgga ctttgtcacc gattttcaat
    38161 tcatttatgt ccacgcttat tttttcagca gtagcattta agatcactcc gtcattgctg
    38221 aatgacgttc ccatattcgc tggatcttct ttggccatga catcgacgag tttcaactga
    38281 agcgtttctt cttctgcttc tacttgagtg atactttcaa tcgttccgac aaatatgctt
    38341 tgtctctccg ttttcgtact gcttgttttt actgtttgac ccgcatcaat cgaatcaaca
    38401 gaacctgtgt tgctatctgc gctaccgcag cctgctaaaa gaaaaaatag accaactaca
    38461 gatatactag aaaaaatagc cctcataaaa aatcctccgt ttaagtttct gcattacttg
    38521 ttgcggtgat tgtctccggt catttctgtc catataatga ttcgctgacc tgcttttact
    38581 actaggtcaa atgtcagttg gacttgtcaa ctgttcaacc attgcgtcgg ttgcgaccag
    38641 tcatttcgat ttcttttgtc aaataacgta tgcgttccat tatccgtttc gcttttagcg
    38701 gctcttcatc tccacgttga gtgacggaca aatgctgttc caattcatcc attgtaaaat
    38761 tcgatgagaa gaaggttggt aactgttcct gcatccgata ttgaaggata acaccgaata
    38821 cttcgtctcg tatccaacta ctcatcgcat ctgcaccaat gtcatcgatc atcaaaatag
    38881 gtgaacgttt cactgcatct aacttttctg ccacttgatc ttttccgatt gcctgtttca
    38941 tctcaacggc aaatgtcgga aaatggacta gcgtcgtggt gaatccagca atcgctaatt
    39001 ctctcgctgt tgctcctaat aaatatgttt ttccaatacc aaaacttcct actagataca
    39061 gtcctttatg ataccctttt ggatcagcag tatatttctc tacaaaatct agggcttcta
    39121 agctcgcata tcctcgtcca tctgtacgtt caaagttttc aaaagaagcc gattgaatat
    39181 cttttggcat atccattgct tttactcggc ttctgatttc ttcttgtctt tgccgtgcta
    39241 acaatgcttc cgttggtaca taagtgacat ccacataatg aaaattcaac gtcagcttcg
    39301 gctcatagcc gggagcgatc atcgttggat cgtttttttg atatttacgc ttttcttgca
    39361 cgaattcata caatttagcg taactttttt caatatccgc ttgtgtcaat tcttcttggt
    39421 gtgcgcttag aaaagcttga acatccgcat catttaagac ttcagcgatc atttcagcat
    39481 acttttcttg ataattgcgt ttgctgatga tcctcttcaa ttctttgcct acgtcttcca
    39541 ctagatatca ccctcctttt ttgccaggta ttctttaaaa cggcggtcaa tctctgcttt
    39601 tttctcgctg ctgatctgtt tttcatcttt tggctggttg acccaatcag gaagcttttc
    39661 ccgtcgaaca tttcgcttgc cagtttgata atttgtttgc ttcttttgtt ttgattgttt
    39721 tgcttgatta cttatttgcc gaatgtgctg gatcgcttct tctgctgtgg cgattttctt
    39781 ttgtgcccat tcatttgcca ctgtatttac ataactggca tttaagctag cattattctt
    39841 gatcactagt acataattca gcaaaacatt gatcacgcta ctggaaagac ctgattgaga
    39901 aacaagatct tgcagtaacc agttttcttg tttagaaaca tatccgcctt tttctgactt
    39961 gattgcttcc agatatttca tcggtggaat gctctgtgct tcttttatca gctgaagcgc
    40021 tgcgtttgtc aaatgttcgt ttgacgaggg ttcattactg ttttcagaaa ccgcaactgt
    40081 ttcttttgtt tgcttcaccg gacgtctctg cattgccaaa atggcttttt gcagttcttt
    40141 ttcagatact ttttgttcac taaagtcata agattgactg atcagttcgc caagtgtcaa
    40201 ttcatcgaaa ccgtaaagct gatgatacat gtcgacttgt ttgatgattg gatcatcaag
    40261 acttgcaata tgtttttttg ccagccaatc attcaaaaac tgccaatcga atgtcgaatc
    40321 tgcttcaagt aatgctactg attcagattc ttcaaaagat ttattcagcg attcgatgtt
    40381 ttcttgatta gcagcgtaca ctgcttcatt gaagcgataa acatctaaaa atttttttgt
    40441 gattttttga tatccctctg ctgtatgcct tttaggttca aagaattggg taagcttctg
    40501 gaatcgtcgt tcaccaactt tttccagaag aagaaaagcc agaacagagt ctttgaaaaa
    40561 tgctttagga atgagtggtt ctaacagctc ataaataaaa cgaggtccca gatcactatc
    40621 ttctttaaaa taaacatcca gtaagccgat tccttctagc ttttttcttg catcataaaa
    40681 ttgcgggata ccgcagccta gactactaag cagatctgca tgcatccctt caggaccttt
    40741 gccagattct tgagagattt ctgataacag ggtgaaatag atactcaagg cttctccgcc
    40801 gattagtggc tgataaagat acagcaaggc gttcttgctg tcttctctca gcggagccgc
    40861 ttttcttcct tggtaaatat tttttggttg aagctctttc caagcatttt ccaaggaaat
    40921 gcctcctttt tgtttcttaa ttgttttctt tattattctc tttattattt tcttttgctt
    40981 tatccacgat atcttgcaac tcttttaaga aaacagacat atctttgaac tggcgataaa
    41041 cacttgcaaa acgaatataa gcgatttcgt ctaaatcaac taaatcttcc atcacatatt
    41101 caccaattag attcgtcgat acttcgtttt ctccgagact gcgtacgcgg ttttcgacat
    41161 ggttgacgat ttgttccatt tgttccatag ctacaggtct tttttctgct gaacggatga
    41221 gtcctcttaa aattttatcg cgattgaact cttcgcgttc cccgtttttc ttgattacta
    41281 ataacggagc agcttcaatt cgttcaaatg tagtgaatcg gaacgaacaa ttctcacatt
    41341 cgcgacggcg acggatcgca cgtccatcat ctgcttgacg gctgtcgatt actcgtgaat
    41401 tattatgttg acatctggga catctcattt tgtctcacct cttgcatcgc attttatata
    41461 aaggatgtgt ctttctttac aactagttgt attataacac gaaacaaatc acaaacgcga
    41521 ctgtcctacc caaaaaacac ggatataaga acaatttttt ctactttcat gcgtaattgt
    41581 gtcccctgtc cttactaaat ttcaattttc caatactgat caccttgatt actgagaaat
    41641 aacttacaca aaagctgatt tagtcatcta gcacaaaaaa cttgaagcag atgaacacct
    41701 tctcatctgc ttcaagtcac atctcgattt tttactattt tataacttct cttcgattcc
    41761 tgtatagtgt ttcattcgct gttcctcgat tttttgctca tcgggaatgt ggtcactttc
    41821 tgggagcggt gttttactac gatcaggtgt cggatttttt cgttcttgag tcaaatcttg
    41881 ccgcttcgct tcttcttctg cctgctggtc ttctatttca tcgattcttg cttggtactg
    41941 cttttctttt tcttctgctg tttctgattg cgtacgacct tcatcgatta gattttcttt
    42001 attgagcatc tcgccgattt tttcttttgc gttacctacg actttatcct tttgattttt
    42061 taatgatccc atcgctatcc ctcactttct atatactcag gatagcaaaa ttgacacaga
    42121 tactcaaaga aagcgattag caaaaagcca attttcgatt tgtcccacaa gtatttcttt
    42181 tgttccattg ttgtcgaaca aaatatccgc acgctgcttt ttttcttcga ttggaagttg
    42241 actactaatt ctctgctgcg cttgttctag cgtcagttga ttacgtttca tcaaacgctg
    42301 aagctgagtt tctggcgtag tataaacgac agctactgca tccatatagt ggtcataatg
    42361 accttcgtag agcaatggga tatctacaat tactaaagga tgattggaac ttagagaggc
    42421 gatttgatct tttatcgctt ttcggataaa cggatctaat agctgatcaa gtttttgccg
    42481 tttttccttt gatggaaaaa caatcatccc aagtttccct cgatctagtt gcccatcagg
    42541 ctgcaatacc tcttggccaa acactgatac aattttcctc agagcaagcg ttccaggctc
    42601 aacagtttct ctggcaatca catcgccatc gatcaaagga aatccatgag actgaaagac
    42661 tttggctgcc gtacttttac cggtagctat tccacccgtc aaacctaaaa caaatcctac
    42721 tttgcgattt ttagcctttg acaattcgga caatagtgcg tccccctttg tgccactttt
    42781 gttttcacga tcggcgttcc acaacgtata catggtttgc cagtttgctg atagacatgc
    42841 agtgctactt gaaatccgcc tgattctcct aatgcattca aataactgcg aatcgtggta
    42901 cctccggctt ctattgcacg atccaaaaca tcaatgatcg atagacgtaa ttgttctacc
    42961 tctttggggc gcagtgtgtt cgccggttgt tctggatgga tcttcgcttc ccataatgct
    43021 tcatctacat aaatattacc tagaccagtc actagacgtt ggtctagcag caatggcttg
    43081 attgctttgt gggacttttt cagtccattt gcgaaatctg ctaataagaa gctatctggc
    43141 aaaggctcag gtcccaattt catgattcct cggtaagttg cacttgctcc tttttcgacg
    43201 attgtcatac gaccaaattt tcgtacatcg ttgtagcgaa gttggcttcc atcctcaaaa
    43261 aagaaaagta catgcgtatg tttatcgatt ggtgtatttt ctttcgtaaa ttgatatttt
    43321 ccttccattc gcaagtgtga gatcagttca caatgatcga gatgaaaaat taaaaacttc
    43381 cctctacgtc caatggattg gatggtttcg ccaacaagag aagcctcaaa gatgggtgtc
    43441 tctggttgct cgatgatctt gggccacaag acctgaactt tttgaatcgt ttttcctact
    43501 actaaccgtt ccaatccttt acgaacggtt tctacttctg gtaattctgg cattttttct
    43561 tttcaccttc attatattgc tctgtcttaa acaatgttta ttttgcttcg taccacgttt
    43621 ttccccaact gctgtcagtg atcagtggta catgaagaga aacagcttgt tccataactt
    43681 ccttcaccaa tttatctaat gattcaagtt ctttttccgg tacctcgaag acaagttcat
    43741 catgaacttg taacagcatc gtagcttgaa gacctgtttc ttttaatcgt ttatccaatt
    43801 cgatcatcgc gattttcaaa atatcagcag cacttccttg gattggtgta ttgatagctg
    43861 ttcgttccgc aaaagaacgg atattgtaat ttcttgaatt gatgtctggc agatatcgac
    43921 ggcggtgata cagcgtttcg acgtagcctt ggtcttttgc ttcgcgtacg attctttcca
    43981 tatattcttt gacacctggg tatttttcaa agtacgtatc gatgtattgc tgtgctgctt
    44041 ttcggctgat tcctagattt tgtgaaagtc cgtaatcaga gatgccatac acgataccaa
    44101 aattcactgc ttttgcctgc ctacgcatat ttggtgtgac atcctctgcc ttttcgattc
    44161 caaatacacg catcgctgtg ctggcatgga tatcttgacc ttctaggaag gcagctttca
    44221 aatgttcatc atttgagata tgcgcaagta cgcgtaactc gatttgtgaa tagtcagaag
    44281 aataaatcag ccagtctttt tctctaggaa caaatgcctg acgtatcttt ctcccttcat
    44341 ctaatcgaat cgggatgttc tgcaaattag gatcaacaga gcttaatcgt cctgtttggg
    44401 tcaatgtctg gacataacgg gtatgcactt ttccatctga ttggatcact ttcagcagtc
    44461 cctcgacata ggtagattga atttttgcaa tttgacgata ggtgaggata tcctctacga
    44521 ttggtgcttg ttcacgaagc tgttccagta catctacggc tgtggaatat ccagtttttg
    44581 tttttttgat aacgggtaat cccatttttt caaataagat cactcctaat tgctttggtg
    44641 aattcaaatt gaattcttcg ccggcttctt cgtagatttt ttgttcgatc tctcttaagc
    44701 gagcagagaa ctcgcccttc atttcttgta agcgagtcgc atctactttg atcccgctta
    44761 tttccatctc tgccaaaatg atagacaatg gcagttccat tttccggaaa agatcttctt
    44821 ggtttttttc tgccagttct tggctcaatt ttggtgtcaa cgcattgatt gcagctactt
    44881 ttcgcgctaa atgagcaaaa aagagttcct cttcttctgg taaacctttt ttcgcccctt
    44941 tgccgtatac agcttcatct gattggatat cgttgtatcc gtaatgagca gctattcctt
    45001 cgatatctgt gcttttttca ttcgtatcga gaaggtatgc acctagcaag acatcaaaat
    45061 cgattccttt cgtttttcca gtatagcggt ttaaagcaac ataagttcgt ttcgcatcat
    45121 atactttttt cagccgagtc ggatcagtga tccaagaatg gaacgctcgg ttttcgaata
    45181 cagatagatc gttcgtgaca taaattttct tttctgtccc ccacgctact ccgacgattt
    45241 cctctgtatg ataattgtcg cccatcattt ccacgtacaa cgccatatcc gtagtaaaca
    45301 tttcctcttt gaattcatga acgacttcaa acaaaatatc atccattttt acttcttctt
    45361 ctacgatatt caatttagaa aggaactgat tgaaatccat ttctttataa aacggaacga
    45421 gtttatcgag atttttccct tcatacttca atgagtcgat agagacatcc acaggcgctg
    45481 aagtattgat ggtcgccaat tttttcgata agaaagcctg ctctttgtca tttatcagat
    45541 tttctttcat tttgctttgc ttcatttcat cgatatgttc ataaattcct tcgactgagc
    45601 catattgttt caatagcttg atagctgttt tttcaccgat tttagtcaca ccggggatat
    45661 tgtcagaggc atctccggct agtcccttca tgtcaataat ctgttttggc gtcaacccat
    45721 catatttttc cgctacatgt tctggtgtat agctttcgat atcgctgaca ccttttacag
    45781 tgatatccac ttttacttct ttactagcta attgagtcaa atcccgatca cctgaaagca
    45841 cgacgacatc aaattgatct ttgtctactt tttctgccaa agtaccgata atatcatctg
    45901 cttcataatt gggcaattcg taatactgta cccctagtcc tgtcaacaaa tcacgtatat
    45961 atggcatttg ttctttgaat tcgccaggtg ttttggatct tccacctttg tactccgcat
    46021 acatttctgt ccgaaaagtc gtttttcctg catcgaacgc aaccagaaca tgagtcggtt
    46081 gctctttttg cataacattt tcaaacatcg tattgaatgc atagattgca tttgtatgaa
    46141 gaccgtttct atttttgaaa cgttcaagag aattgtgaag cgcaaagaat gcgcgaaaag
    46201 ccacactgtt cccatcaacg agtaataatt tattttttgt catcttctta actccttact
    46261 gtaaagttct tattcattct aacaaagaat cctgcggaat gcgaaaagaa aaagcgggac
    46321 cataccaaat aattcggtat agccccaact gatgaatttt tttctttttt agttgttccg
    46381 tccaaagatt ctcagaaacg ccaagaacaa gttgataaag tccaaataca gcttcaatgc
    46441 catgaataca gcaattccac tatgtgctgt tccgtttgtc tggacataga tattacggat
    46501 gctttggtta tcataagctg taatccctgc aaagatgacg actaacagta gggagatgaa
    46561 cagctcaact acgctacttc tcaaaatgaa gatgttcaag actagtgcaa tgatcactcc
    46621 gattaaagct gcctgtcctg ctcttccaac cccgctaaga tctctttttg ttgttactcc
    46681 gacaacagac atccccatga acatcaaagt agctgtcaag aaagcttgcg ttactgttcc
    46741 aatatcgtag tatgcaagag taaccgctaa tattatgcca ttcaaaagag agtaggcaat
    46801 aaatccaccg attgctaagc tcggattctt ttgtgctttt acgcctagaa ccattactaa
    46861 aatcagttca acgatccaca gacccacaaa gcctagtgga aaattattga taaaagttgc
    46921 taattgcaaa ggaaataccc aaagagccaa gtaagaagtg actgcactaa gggcaagtcc
    46981 catacctaaa aatccataaa cttttgcata aaaacgattg atacctgaca ttgatgtact
    47041 tggattattc attcaattca ccttcacttt cgattgctat ttccaattca ggaacttgtg
    47101 gcggttggac cataacgaca gcatccaaca cacgatcttc gttcgtattc tccgcttcta
    47161 cagaaatcgt caccacatcc ttcatcttat ctacttttat gacctcaaac tgaaaagtca
    47221 gtgtttcata atggaaaacc ggttcaataa aattgacaga aaaattgaca acatgtgatc
    47281 ctggtccagg aagatgcttt gaaattgcac ttgtgatgat ccccatcagc atcactgatg
    47341 gaacgattgg ctgttgatat tctgtttttt gtgcaaagtc atgttggata tacaaaggat
    47401 ttgcatcatt cgtcaatccc aaatacagca aaagctggtt atcttcaata gattctgtta
    47461 aggataacga gtcgccttcc tcgatttcat ttattgtttt tcctagtttt ctcggcttac
    47521 ctatgttcaa gatagaaacc ctccagttta tttttaatca tacattattg taccaaaagt
    47581 agcgtaaaag gcaagttctg ctcccttgat ttaacagatt cctatagttt acagaggtcg
    47641 tgaaaaagct gtcctgcatc aaataataag aacagccaag aaaaaacagc ttctcatgtt
    47701 tttgatttga ttgggcttat taccgcagat gcaagctttt gaacaccgtt tatcataagt
    47761 tgcaaaaaag ggggcttggc aagatttttg ccactccccc tctttctatt tttataagga
    47821 cactgcagta attgtattac agtacatttt tcgtcgtgct gcttaaaagt ttttcatacg
    47881 cttgttcaaa tttttgaaca tctccagcac ccatgaagat tactactgca tcatgataat
    47941 ctaacaatgg agatacatta ttttctttga tgacttctcc accttttttg attttcgctc
    48001 caaggtcttc aatcttaacg tttccttgtt cttcccgagc cgaaccaaaa atatcgcata
    48061 agtaaacttt atcagctaaa tcaagtgctt ctgcaaattc atccatcaat gcaatcgtac
    48121 gcgtaaacgt atggggttgg aaaactgcaa ttatttcttt atctggatat ttttggcgtg
    48181 cgccatcaat cgttgctttg atctctgcag ggtgatgcgc ataatcatcg actacggtca
    48241 tgtctgctac gatcttttca ctgaatcgac gttttactcc tgggaaagtc agcatttctt
    48301 ctgcaacttc tttcagatcc agtttttcga aataagctac tgcaataaca cctaaagcat
    48361 ttaagatatt gtgtttaccg aatgctggta cagtgaagtg cccaacaaat tcatccccat
    48421 gatagacatc aaaagcagaa ccagaagttg tgcgttcgat gtttcgtgct tggatatcat
    48481 cgttttctgt gacaccataa taataaatcg gcacatttgc ttttaatttg cgtaaataag
    48541 catcgtcacc atacgcaaaa atagcttttt tcacttgtcc agccattgtt tggaaagctg
    48601 taaatacatc gtcgatactt gtataataat ccggatgatc aaaatcgata ttggtcatga
    48661 ttgcatagtc tggtgaatag gcaaggaaat gacggcgata ttcacaggcc tcaaatgaaa
    48721 agaattccgc ttgcggatca ccatgtcctg ttccatctcc aattagataa cttgttggac
    48781 ggacaccact caatacatga gagagcagtc cagtcgtact tgtttttccg tgagagcctg
    48841 ttacagcgat actcgtataa ttttggataa aatgaccaat aaagtcgtga tagcgaatca
    48901 cttccaaacc taattctttt gctcgctgga tttcttcatg tgaatcagga aatgcatttc
    48961 ctgcaatgat cgtcatccct ggttttacat tatctgcgtt aaatggcaga atcgtgatat
    49021 ttgctttttc taaatctctt tgtgtaaaga aatatttttc aatgtctgat ccttggacat
    49081 ttagaccttg ttcatgtaaa acaagagcca aggagctcat tcctgaacct ttgataccaa
    49141 caaaatgata caatttattt tgattttcca tttgaatcct cttctcttat ctagcgtcca
    49201 ttcttttagc tattacattc aacacgctcc tcattatctt ataaacgatt gaaaatttca
    49261 accaaattta tttggaaagg aggattcgct tgctaaaatg gtcaagtacg agaaaaatcc
    49321 gtttttattg actctttaat tctggtgtaa gaagaccaaa cttcctctga tatatcaatg
    49381 tgaggaataa aatcttttcc tttcggacca tagccgttct catcgtcatg gagacgttcc
    49441 atttctccta atactagcgc atggaaatat tccttaggcc attgatgatt cagttctttt
    49501 gctgtaaaga tgatctcttc agaaaagtga aggatttccg gcacaaatgg cacagcattc
    49561 gcgaaaatag tttgttcatt ttgccatact ttctcaaaag ttgctcgagt cctgcgttgc
    49621 agtgtcggat cattcatcaa taagattttt tctggtacag ctcctaagga atgaagaatc
    49681 tccagtgaaa aagtggcgtt ctctccagaa tttgtagact tcgattctat cagaaaagcc
    49741 ttatcgggca aatcatagac ttcttttaga tactgacgac agatttcact ttcactcatg
    49801 ccatcttcaa aatgaaatcc ttgcttttca aaattctcat aaaggattct agtcgcatgc
    49861 cccacccctc cgaccaaaaa caattgatct acttggccat ttaaatacat ttgtgctgct
    49921 ttatcagcta agataggaag acagtttcca gctaaaataa gtgtaggtgc cgtcagttcc
    49981 tcaaaatttc cctcttcagg tgcggctaaa aaatttaata gctgattcca gtcttttaga
    50041 ttcctctgct cacttttttc caaaggcctt ctatcctttc atctgatttt ttgtgcttca
    50101 ttaaagtctt ctctttttgt ctcaatccat ttgatttctc catgacatct tccacaacga
    50161 tatttgcgta catcgattct tctcttgcgg cagatcgtgg cagaacaatc ttggcatcga
    50221 taatactcca tctttatctg tttcttcgtc aaggctggtg catagcgcaa ccctcctgtt
    50281 tttttcaaaa gatacttgaa atccatatct ttatgctgat accctcttcc agctagatga
    50341 agatgataat ggcaaagttc atgtttgatg attcctgcaa tcgtctcttc gtctgactct
    50401 tcaaatagct tcgcattaaa atctaaatga tgatcgttta aatgatagcg cccgccagtt
    50461 gtacgtaagc gtggattgaa cattgcttgg tgcttgaaag gaagctggaa gctttttaat
    50521 gatatctctt ctactaactg ctgcagttct tcttgtgtca atttttgtta ctcctcattg
    50581 ttactcttca taaggggaaa gcatcgtcaa actgattctg ccttttttcg tatcgacttg
    50641 ttctatccaa actgttacga tatctcctac tgacacgaca tctgtaggat gcttgacgaa
    50701 ttttttgctg agtttcgata tatggacaag tccgtcttgc ttcacaccga tatcaacaaa
    50761 tgcgccgaag tcaatgacat tacggaccgt tccagtcaat tccatccctg gtttcaagtc
    50821 ttccatactc aatacatctg tacgtaataa gggcgctggc atctcatcac gcatatctcg
    50881 tccaggttgt gtcagcccag ctaaaatatc ggctaacgtt tcttctccga ctgataggct
    50941 ttcagccagt ttctctacgg ataatcgtgt caatttttct actgcttctt ctgtaccaag
    51001 ctctttttct gaaagttgga ctgaagttaa tatttctttt gcaatagaat aactttcggg
    51061 atggatacct gtgttgtcca aaatgttttt cccaccaggg actcgcaaga atccgattgc
    51121 ttgttcatat gcttttggtc ctaagcgtgg cacttttttc aattgagttc gtgcggtgaa
    51181 ttctccgttt tcttcacgat aaatgacaat gttttgggca gttgttttat tcaatcctga
    51241 tatatgctgc aagagctgcg ggcttgctgt attcacatcc acacctactt ggttaaccgc
    51301 tgtttccaca acaaaatcca attgttcagc caaacgtttt tgagaaacgt catgttggta
    51361 ctggcctact ccgacagctt taggatcgat tttgactaat tcagctaaag gatcttgcag
    51421 gcgacgggca atagaaacag cacttctttc ttcaacttgt agatgcggaa attcttttcg
    51481 tgcaatttcg ctagcggagt aaacagaagc gcctgcttcg ttgacgatag cgtaataggt
    51541 tttatggtcg gcggatttca actgctcagc cacaaaaagt tcagattccc gactggctgt
    51601 accatttccg atagctacca tatctacttg atatttatta attaattgga taaaagctgg
    51661 tccagcagct tctctttttg cttgcgctgc aggtttatga ggataaataa cttcgattgc
    51721 taaaactttc cctgtcgcat ctacgactgc tagtttacat ccagtacgat aagccggatc
    51781 aaatcccagg acaactttac cttttagtgg cggttgcaac aaaagattac ggaggttttc
    51841 gccgaaaata gcgattgctt gctcatctgc cttttcagtc aattcattgc gcagttctcg
    51901 ctcgattgct ggctgaataa agcgtttata actatcctga taggcttctc tgacgaatga
    51961 agaagaagga gaggcaggat ttttgactat ttggcgatcc aagtaagcta aaattgctgc
    52021 ttcatctact tgaaagaata ctttcagaat atcttctttt tctccacgat tagtagctaa
    52081 tattcgatga gaaaccattt tatggacggg ttctgcaaag tcataataca tttcatatac
    52141 gcctttttca tctgcttgct catccttgac attgctgaca tacatacctt tgttatacgt
    52201 ataagaccga atccaagtcc gaaattttgc attatcgctg acttgttcag caataatctc
    52261 atgagcaccg tgcaacgctt cttcggcaga agagacttct ttttctttgt caatatattt
    52321 ttctgcttca gattgcacct ctccatctgt aagctgcatc agccacttcg cgagtggttc
    52381 aagtcctttt tctttagcaa tcgttgcttt tgtccgtcgt ttttgtttgt atggacgata
    52441 caaatcttcc acttgttgca ttttcacggc ttgggtgatt tctgcttcta actcgggagt
    52501 cagtttccct tgttcatcga ttagccgaat cacttcagtt ttacgttttt ctaagttttc
    52561 aagataggca taccgctctt cgatttcacg aatctggact tcatcaagac ttccagtcat
    52621 ttcttttcgg taacgcgcga taaaaggaac agtgtttcct tcatttaaca aattaagtac
    52681 agtagtcaac tgcttaggac gatagtctga aagttctttt tgtacaagct gaatgatcgt
    52741 ctggttcaaa ttttccgtca ttaaaatgcc tcttttctgc taaaaattgc ttctttattc
    52801 tatcatactt tttatcctgg gttaaactac tgaaagaaac aatattctct ataaaaataa
    52861 aaagagccgc aacaaagtca cagctccttc attctttcca ttagtcagat tccatgccaa
    52921 ggtccttcgt tgttctgata ttcaatatct tgaagaatac cttccttgtc aaaaacaacc
    52981 ccatgcaatg accctccgaa tacagcccca ccgtccattc cgattttatg atcctgaatc
    53041 cagaggtctg ttgtctgcat atctccatgg agcattggtg taatcgtatg tccaaataca
    53101 attgtcttac ctgtattgtt tttcccttca tgaaatggtt cccttatcca aataaagtct
    53161 cttagacttg tcttacgcca gtctttcttt gtcaaatcca caccggcatg gacaaaaaga
    53221 taattttccc attcataata caaggggcgc tggactaaaa atttaatcag ttctgaatat
    53281 ctcgattgga tcatcatcga gatttcagtt ggggagtatt cctccgatgc ccctgggtga
    53341 atcaggcttt caaacgtttc ttttccacca tttctcatat aaggcgcata ccagtcctcc
    53401 ggtgattgca gaaattgcaa aaaatattct tcatggtttc cccggaggta aatggctcct
    53461 gtttcttcga ctaattgctt tcctaataaa aaacattctt ttgtacgagg acctcgatca
    53521 ttgagatccc ctaataaaac aagctgatgg atctttggat cataatagtg aataagtttt
    53581 tcaaaaagct catgtttgcc atggacatcc ccaatagcat aaatcaatga tttcatgtgg
    53641 atcaccatcc tcaacgctag tatagcgcat attggaaatg gtcgacaatc aaaactattg
    53701 ttttctattt aattaaagct gtaaaccgca gttctgcttc ttctggagag attccatggt
    53761 cttgaacata gcgattgcgc ggatgtttcg cacactctat gctacatgct cctaaatgtt
    53821 tttcttcatt ttcaatggaa gcaagaatct gacgattgca ctcaggattg ctacaattga
    53881 tatatcgttc acatggtgtt ccgtcaaacc agtcgcgacc gacgatttgt ttatctactt
    53941 ggttgatatc gacagcgatc cgttcatcaa acacatacat cttgccgtcc cataattctc
    54001 cttggacttc aggatcctta ccataagtag caataccgcc atgcagctgt ccgacatctt
    54061 tgaatccttc acggacaagc caaccagaaa acttctcgca acgaatgcca ccagtacagt
    54121 aggtcactac ccgtttttcc ataaattctt ctttgtgctc gcggatccag tctggaagct
    54181 ctcgaaaact gcgtatatct gggcgtacag cccctcgaaa atgtcccaag tcatattcat
    54241 aatcattccg tgcatcaatc accactgtct gttcatctaa aatagcttct ttaaattctt
    54301 gcggtgaaag ataggcacct gtcacttggt ttggatcaat gtcctcttct agctttaaac
    54361 ttacaagctc tggacggtat cttacaaaca tcttcttaaa ggcatgtcct tcgcttggat
    54421 cgatcttgaa catcgtatca gcaaatcgag gatcagcttt cattgctttc atataggcag
    54481 tcgtgttttc aacggttcca gacacagtac catttattcc ttcttcagct actagaatac
    54541 gacctttaag atccaaagat ttgcaaaagg ctaaatgatt tttggcaaat tcctctggtt
    54601 cttcgatttt tgtataattg taataaagca gtacttgata atccatgtat ttcttccttc
    54661 cttcttttcg ttcctaaaca gtataaagaa ggcgttcgca tttgacgatt tatttgcttg
    54721 tgtataatta cgcatggaaa gttttttgtt tacataatat tattatttga attaaaaaga
    54781 cttcgctttc ctgtttcgca cagaaaaaca aagtctttat ttctattatt tacctgtaac
    54841 cgcaaagtag ttgccatcgg gatcagcaaa gttaaagaca tattcttctc caagctgtac
    54901 catatcacct aatgtgactg attgcgtctg cattttttta tatagtccaa agatatcttt
    54961 actgtaaaac atcaaagatg gggcactagt agctgtgcca ggtgaatggg actcaacaaa
    55021 tgctcgatca tataacagaa atgaggtttg actgtcctca actcctactt caattataag
    55081 cgtcccatcc atttcttgac gatctttttc cgcaaagcca actgctctcc aaaacgttgc
    55141 atttttttct acatcgtcca cataaagcat gatttttatt tcattcgtaa acatct
//
LOCUS       DAYYAR010000004        54877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000004 DAYYAR010000000
VERSION     DAYYAR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.004"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..815
                     /locus_tag="ACHAT0_000186"
     CDS             135..815
                     /locus_tag="ACHAT0_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8450164.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            905..1939
                     /locus_tag="ACHAT0_000187"
     CDS             905..1939
                     /locus_tag="ACHAT0_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8450165.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1953..2288
                     /locus_tag="ACHAT0_000188"
     CDS             1953..2288
                     /locus_tag="ACHAT0_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450166.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2272..4752
                     /locus_tag="ACHAT0_000189"
     CDS             2272..4752
                     /locus_tag="ACHAT0_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450167.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            4808..6367
                     /locus_tag="ACHAT0_000190"
     CDS             4808..6367
                     /locus_tag="ACHAT0_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8450168.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6364..7728
                     /locus_tag="ACHAT0_000191"
     CDS             6364..7728
                     /locus_tag="ACHAT0_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8450169.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7899..8969
                     /locus_tag="ACHAT0_000192"
     CDS             7899..8969
                     /locus_tag="ACHAT0_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8450170.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            8984..9652
                     /locus_tag="ACHAT0_000193"
     CDS             8984..9652
                     /locus_tag="ACHAT0_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450171.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9672..10250
                     /locus_tag="ACHAT0_000194"
     CDS             9672..10250
                     /locus_tag="ACHAT0_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8450172.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10651..11562
                     /locus_tag="ACHAT0_000195"
     CDS             10651..11562
                     /locus_tag="ACHAT0_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8450173.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11650..12567)
                     /gene="rnhC"
                     /locus_tag="ACHAT0_000196"
     CDS             complement(11650..12567)
                     /gene="rnhC"
                     /locus_tag="ACHAT0_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8450174.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            12769..13206
                     /gene="zapA"
                     /locus_tag="ACHAT0_000197"
     CDS             12769..13206
                     /gene="zapA"
                     /locus_tag="ACHAT0_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8450175.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13265..13813
                     /locus_tag="ACHAT0_000198"
     CDS             13265..13813
                     /locus_tag="ACHAT0_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8450176.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            13928..16288
                     /locus_tag="ACHAT0_000199"
     CDS             13928..16288
                     /locus_tag="ACHAT0_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8450177.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16375..16689
                     /gene="trxA"
                     /locus_tag="ACHAT0_000200"
     CDS             16375..16689
                     /gene="trxA"
                     /locus_tag="ACHAT0_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8450178.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            16871..18649
                     /gene="uvrC"
                     /locus_tag="ACHAT0_000201"
     CDS             16871..18649
                     /gene="uvrC"
                     /locus_tag="ACHAT0_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8450179.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            18794..19693
                     /locus_tag="ACHAT0_000202"
     CDS             18794..19693
                     /locus_tag="ACHAT0_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450180.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19686..20948
                     /locus_tag="ACHAT0_000203"
     CDS             19686..20948
                     /locus_tag="ACHAT0_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8450181.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21109..23007
                     /locus_tag="ACHAT0_000204"
     CDS             21109..23007
                     /locus_tag="ACHAT0_000204"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8450182.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23337..24056)
                     /locus_tag="ACHAT0_000205"
     CDS             complement(23337..24056)
                     /locus_tag="ACHAT0_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8450183.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    24246..24293
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24393..24725
                     /locus_tag="ACHAT0_000206"
     CDS             24393..24725
                     /locus_tag="ACHAT0_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450184.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            24712..26715
                     /locus_tag="ACHAT0_000207"
     CDS             24712..26715
                     /locus_tag="ACHAT0_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8450185.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            26734..27204
                     /locus_tag="ACHAT0_000208"
     CDS             26734..27204
                     /locus_tag="ACHAT0_000208"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8450186.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27225..27809
                     /locus_tag="ACHAT0_000209"
     CDS             27225..27809
                     /locus_tag="ACHAT0_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8450187.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            27824..28810
                     /locus_tag="ACHAT0_000210"
     CDS             27824..28810
                     /locus_tag="ACHAT0_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8450188.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            28800..29111
                     /locus_tag="ACHAT0_000211"
     CDS             28800..29111
                     /locus_tag="ACHAT0_000211"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8450189.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29133..30914
                     /locus_tag="ACHAT0_000212"
     CDS             29133..30914
                     /locus_tag="ACHAT0_000212"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8450190.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            30907..32283
                     /locus_tag="ACHAT0_000213"
     CDS             30907..32283
                     /locus_tag="ACHAT0_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8450191.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32290..32925
                     /locus_tag="ACHAT0_000214"
     CDS             32290..32925
                     /locus_tag="ACHAT0_000214"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8450192.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            32955..34310
                     /locus_tag="ACHAT0_000215"
     CDS             32955..34310
                     /locus_tag="ACHAT0_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8450193.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34502..35896)
                     /locus_tag="ACHAT0_000216"
     CDS             complement(34502..35896)
                     /locus_tag="ACHAT0_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8450194.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            35988..36959
                     /locus_tag="ACHAT0_000217"
     CDS             35988..36959
                     /locus_tag="ACHAT0_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8450195.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            36956..37738
                     /locus_tag="ACHAT0_000218"
     CDS             36956..37738
                     /locus_tag="ACHAT0_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8450196.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(37907..38278)
                     /locus_tag="ACHAT0_000219"
     CDS             complement(37907..38278)
                     /locus_tag="ACHAT0_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8450197.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38412..38855
                     /locus_tag="ACHAT0_000220"
     CDS             38412..38855
                     /locus_tag="ACHAT0_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8450198.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(38898..39707)
                     /gene="yidA"
                     /locus_tag="ACHAT0_000221"
     CDS             complement(38898..39707)
                     /gene="yidA"
                     /locus_tag="ACHAT0_000221"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8450199.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(39726..41093)
                     /locus_tag="ACHAT0_000222"
     CDS             complement(39726..41093)
                     /locus_tag="ACHAT0_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8450200.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41077..41907)
                     /locus_tag="ACHAT0_000223"
     CDS             complement(41077..41907)
                     /locus_tag="ACHAT0_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8450201.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42070..42507
                     /locus_tag="ACHAT0_000224"
     CDS             42070..42507
                     /locus_tag="ACHAT0_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8450202.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42583..43203
                     /gene="rpoE"
                     /locus_tag="ACHAT0_000225"
     CDS             42583..43203
                     /gene="rpoE"
                     /locus_tag="ACHAT0_000225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8450203.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43392..43898
                     /locus_tag="ACHAT0_000226"
     CDS             43392..43898
                     /locus_tag="ACHAT0_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8450204.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            43915..44814
                     /locus_tag="ACHAT0_000227"
     CDS             43915..44814
                     /locus_tag="ACHAT0_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8450205.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            44901..45785
                     /locus_tag="ACHAT0_000228"
     CDS             44901..45785
                     /locus_tag="ACHAT0_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8450206.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(45977..46048)
                     /locus_tag="ACHAT0_000229"
     tRNA            complement(45977..46048)
                     /locus_tag="ACHAT0_000229"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46013..46015),aa:Arg,seq:ccg)
     gene            46108..47442
                     /gene="rpoN"
                     /locus_tag="ACHAT0_000230"
     CDS             46108..47442
                     /gene="rpoN"
                     /locus_tag="ACHAT0_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8450207.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47591..48682
                     /locus_tag="ACHAT0_000231"
     CDS             47591..48682
                     /locus_tag="ACHAT0_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8450208.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            48723..49724
                     /gene="gap"
                     /locus_tag="ACHAT0_000232"
     CDS             48723..49724
                     /gene="gap"
                     /locus_tag="ACHAT0_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8450209.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            49834..51024
                     /gene="pgk"
                     /locus_tag="ACHAT0_000233"
     CDS             49834..51024
                     /gene="pgk"
                     /locus_tag="ACHAT0_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8450210.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51128..51883
                     /gene="tpiA"
                     /locus_tag="ACHAT0_000234"
     CDS             51128..51883
                     /gene="tpiA"
                     /locus_tag="ACHAT0_000234"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8450211.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            51999..53297
                     /gene="eno"
                     /locus_tag="ACHAT0_000235"
     CDS             51999..53297
                     /gene="eno"
                     /locus_tag="ACHAT0_000235"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8450212.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            53696..54835
                     /locus_tag="ACHAT0_000236"
     CDS             53696..54835
                     /locus_tag="ACHAT0_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8450213.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        18226 a         9572 c        11602 g        15477 t
ORIGIN      
        1 gtaaacgatt ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc
       61 ggatgaattc atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa
      121 aggagtgatt tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc
      181 taggcggctt ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta
      241 cctttactga accaacaaat accgtggagc ctcttaaccc tactgatcct tcaaaaccag
      301 tagagccagc agatccggaa aatccagcaa ctggccagac aggtccttta acactggatg
      361 tagttcccga attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag
      421 tagacgcttc aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg
      481 cagatggcca agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt
      541 cacaagtttt gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag
      601 ataacgaatc cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag
      661 cgacaccaat atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg
      721 atccagcaaa tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt
      781 tgacttggaa cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg
      841 cggtgaaaat actataggtg aaggattttt taccgcagtt tttgttgaaa ggatgtggtg
      901 gacaatgaat caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt
      961 catttttccg tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt
     1021 atttccagag aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga
     1081 acaaaatcag acactagaag taattatcag caattcttca gatgaagaac gaacatttga
     1141 ggtctctgtc aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa
     1201 cccaacgtta gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa
     1261 ggaaatcaat gtatcagcac atgcagaaac aactgtacct atagaagtga aaatcccagc
     1321 aaaaagcttt aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga
     1381 aacagaaaat gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt
     1441 cgttttacaa gaaagtcaag agacgataga accggatctg aaacttttat caggagattt
     1501 ggacgaggtc aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat
     1561 tatcagcaat ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa
     1621 tacgtctaat gcatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc
     1681 tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag
     1741 taatgaatgg cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa
     1801 tgaaaattcc gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt
     1861 tgctgcagtt ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa
     1921 aaaaggagaa acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga
     1981 ttattgcaag tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt
     2041 caggtacgat tacattttat gatctaagtc caattccgca tccaagtggt tcagtcaatg
     2101 gcgcatcaaa acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat
     2161 tcccggagac gaatgaacaa aagatccgtt ttctttattt gatcattggt atagaattat
     2221 ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta
     2281 caaaataaat tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata
     2341 tttatctttt caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat
     2401 ccatacacgt atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg
     2461 aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac
     2521 gatggttcaa atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg
     2581 aaactaacaa atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg
     2641 atcatcaatt tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac
     2701 aacgcctact tgtatattgc cggtgccccc atcaaggtta ctagcggaag tttattatca
     2761 gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattgaca
     2821 gatattattg ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt
     2881 gatgttacag ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca
     2941 aacaaaattt tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct
     3001 gttgattttt atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct
     3061 ccagctgcag gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat
     3121 gcattttctg caagtgcaga gataaatacg tggaatagtt acatctcacc aagtgattca
     3181 acaggaaaat ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac
     3241 cttccaggct ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat
     3301 cagttgccat ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca
     3361 caaggagcaa tactgactca tactccagca agcacaagtg gattgaatca gacagccaat
     3421 atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta
     3481 ttggagcagt acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag
     3541 tggggaaagc tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa
     3601 ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca
     3661 ggatggacag atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct
     3721 acttctacac tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac
     3781 gatgtactta ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga
     3841 atcaccactc ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag
     3901 ctaaaaaacc ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat
     3961 acgattcaag aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa
     4021 aaaatcacga tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat
     4081 ggtacaacta tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg
     4141 agcaaaatag gagataccat tcatcttact tacaactatc aattaaatac tgtagatact
     4201 tctttacaat ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta
     4261 gcagatggtg ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc
     4321 cccaaacaag aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc
     4381 cccaaaccgt tgaagacaag ttattatgaa tcaaaaggcg atttttcttt tgatgtgcgg
     4441 gatactcggc ttccaagcac gtctccttgg caactgacag gtacattgac ttctttattt
     4501 aagaatgatc aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca
     4561 aagcaattga ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa
     4621 ggagaggttc tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt
     4681 acgaatgctc aacctggcgc aacgtatcaa ggcatggtca catggaaact gacagctggg
     4741 ccaaccagct aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt
     4801 ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa atgcttgaag gagtggactt
     4861 ggaacttttc agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt
     4921 attagaggaa aaaggagagg tttccgttaa taacgtactt acccgcttat cagttgatcg
     4981 ggctaccttg aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt
     5041 ggaaattgtt atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt
     5101 cgatgtttat tatcattgtt acttgcgcta ttctacaaaa tatcagatac ttctttatct
     5161 gttggaaaat ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc
     5221 tactttgtct cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat
     5281 aaaaagcggg cggctgaccg gtccagaaag tcaaattcgt tatttttatt tccaattatt
     5341 ttggtttgga cgtccctatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac
     5401 ttttttagat ctgctgcaaa aagagtttag ttttgctatt acagaagaag gacgattgaa
     5461 atttgaaata tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc
     5521 agattatagc aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca
     5581 tacatttttg acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt
     5641 ggttttttat cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca
     5701 ggagtttttg ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt
     5761 taaacaatcg atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa
     5821 gattgcgttg acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa
     5881 tatttttggc caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct
     5941 ggttttatgt gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga
     6001 cgaggaaaat gcccgctttg aattatttgc tcgctatgca tctattttac ggacgctgat
     6061 caagcagtta gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc
     6121 ggatatcgtg aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt
     6181 cgaatacgag aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc
     6241 gtcacagaag tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc
     6301 ctatttgaac caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa
     6361 aacatgaaaa tcgtgatcat tggtgcttcc catgcaggca tcacagctgc cctaaatcta
     6421 agaaaacttc aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta
     6481 ggctatgttt caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa
     6541 gttgcccata acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga
     6601 gtaactgaac ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac
     6661 gagaccataa cctatgataa attaatcgta gcgacaggtt cctctcctgt caccttgtat
     6721 aaacaaattg aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta
     6781 cttgccgctt taaaagaagc aaaagaagta gtgatcttcg gtgctggtta cattggattg
     6841 gaattagcag atgctttgag aaataaagga tacattattc atctagttga ttatatgccg
     6901 aatgttttat caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa
     6961 actaagcaaa taaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag
     7021 aaagtagtat ccgtacaact tctgtctcaa acaataaagg cagatatggt cattttttct
     7081 gcccaaactc gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag
     7141 actgtgatag tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat
     7201 atcgtcccag tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga
     7261 gctgtacata tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac
     7321 ctcagacaaa agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg
     7381 acagaagatg aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat
     7441 ctatttccac aatacgacga agaaaacaaa acagtaaatg caaagctgat ctaccatccg
     7501 gatacattag aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg
     7561 aacctgcttg cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat
     7621 tttggatttc tgtctgaata cacctctcgt tttcactacc tcaatgagtt agcttttaaa
     7681 gtactagcta agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg
     7741 tcttttattg tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa
     7801 tgaaatagcg gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa
     7861 ttgttgtata aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa
     7921 ttatgcattc aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc
     7981 tggtattttt tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg
     8041 acaagtggga ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt
     8101 ccatgattac taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc
     8161 aaacacctgg agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct
     8221 tcttcggtat cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg
     8281 ataacgatga tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccatag
     8341 gagatacagt taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc
     8401 atgccaaatt caatgtctca ccagttctct atacaaccat caatgcttac caagaaatcc
     8461 gcttcgaaaa agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta
     8521 aaatcagtaa tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat
     8581 tacctggata taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta
     8641 ttgcggctat tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct
     8701 ttggtgtaat gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac
     8761 aaacattcat tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat
     8821 cccttgttct acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta
     8881 gcttgttgat gttagtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa
     8941 aaatcgatcc gttgaaggca attggataag aaggaggaag aacatgaaag caatagaatt
     9001 taaagaagta gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac
     9061 aaccatgagt atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa
     9121 aagtactttt ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat
     9181 caatgatcaa gaattcagta atcaaaaaga aagcgaacgc gttaaactac gttttaaaga
     9241 aattggcttt attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt
     9301 attattagta gataagatta aacatgaaca tcgtcaaaaa gaagcaaacg aattatttga
     9361 gcagcttggc gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg
     9421 gcaaagggtc gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga
     9481 accaactgct agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga
     9541 atcaaaagac aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa
     9601 atgcgataga atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag
     9661 caggtggaaa aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag
     9721 cgactgactt gtttatgtcc aagggatatc tcggcacttc aacaagagaa atcgcacaaa
     9781 aagttgggat cacccagccc aatctctatc attatttcgg tgataaagaa aaactatata
     9841 cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa
     9901 caagcgaaac gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac
     9961 atctggtcga tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa
    10021 gagatcatct tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt
    10081 cagcaaacca atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt
    10141 tcttagtgtt agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg
    10201 tggcgcaatt gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata
    10261 tagggagaaa atagttaggt gaagaagcaa gatatgacga agatcgttat atcttgcttc
    10321 tttatatagg tagtttttca taaagacaaa tttgttattc tctcatgatt atcagggtat
    10381 atttttattt attatgtcgg tgttccagga agtgttttaa tttaataacg aagaaattaa
    10441 ttgtttgtag aatgaaggaa actatttatt gggtagatga atcaccacga atagtcaaaa
    10501 ggagatttca ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg ttattttcta
    10561 cggttatatc ttttacagcc ccttttttgc ttacttaagc tgattttaag ttttggccct
    10621 tagtctggat ctacgaggtg agagtcaaga atgaaaaaaa taattggagc ttttgtattt
    10681 gcaatcatta tcattattat ctgttttcaa atagatgttt ttggtcaaaa aaagatagtt
    10741 ggacaaacgg tcagcagtac gaacagtacg gttacaaaga caaaggaatc atttgatgat
    10801 acagaacaag agacattaat caaaatcgga aaaaatgacc gtacactata tacagatgac
    10861 tcgctaacta ccccacttgc aactataaac ggcggagagc tggcggagtt tctttctgaa
    10921 acaaaaaatg cttatcagat caagacaaat gatggctata ctggatattt agccctcgaa
    10981 gatggaacca aagtcacaaa gaatatacaa acaaaaccgg aagagctatc tgatgcagtg
    11041 attgtattag atcctgggca tggcggaaat gatactgggg cattaagcaa tgatgaacaa
    11101 acagaagaaa aagatataac attaagtaca gccataaaag taaaaaaggc gctagaggat
    11161 gctggagcaa cggtttattt gacccatact acagatgaat tagtacagct tggagatata
    11221 tgcgactaca gtgaagaaaa aaaggcagat gtctttatca gtttacacgc agactctacg
    11281 gaatatgcga atgaagcaac tggaatcacg acatattatt actatggaca agaggaaaca
    11341 ttagcacaga caattgctga ctccttcact gatctgcctc tggattcaag aggaatctcc
    11401 actgggaact atcaagttct tcgagaaaat cttcaacctt ctattttaat cgagatgggt
    11461 tatatgaata atgatagtga tttaaaggaa ttagtaacag acagttatca gcagcagatt
    11521 gctgcaagtt tgacacaagc attgaccact tattttcagt aataaataag cccagccaaa
    11581 caaaaatagc tcctcatatt tttcgtttgg ccgggcttat tatcgcagat gcaagctttt
    11641 gaacaccgtt tattttttta gtagcttcaa agctttttct gtatttgcaa agtgaagttt
    11701 ggcatatttt tcaagcatag gcatgccgcc tttctccaat agttttgccg ctacttggtc
    11761 agatttcgtt ccagctcctg atggaagggt gattccagct tcagctgatg ctttgtgtaa
    11821 ctcttccaaa aagctggcac gactgatgat agaagcagca gcaacagcta aatgatattg
    11881 ttctccttta gtaacgaagt acagtttttc ttgtaattga tttttttcta aacgcgcata
    11941 ttttttaaaa tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg
    12001 ctcgattttt tttagcagca aatggatcgc ttgattgtgc aaagcgactt tcatccgaac
    12061 ggcattgtat tttggttgga tctcattgta tttttctgga gataagacaa gcagtttata
    12121 ggggatggtt ttttttaaga tgtcagcaag ctggatgatt tggctatctt ttaactcttt
    12181 tgaatctcta acaccaagtt tacgtaattc tgcaagctga tcacgtttca cataagcagc
    12241 acatactgtt agcggaccga aatagctgcc atttccgacc tcatcactac ctaaaacgga
    12301 taattgactg atattttttg gcaaagaaga ggctgttttt gctggcttgg cttgtttatc
    12361 ctgtgcctcc ccccatttat cagcttctat tgctgcattc gtaccttgaa acatcacttt
    12421 tccggatgta taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc tagtataagg
    12481 aatctgttta tttaacaaat aagagcgata gtatgctttc atttgactaa gttggtcgtt
    12541 tgttgctttg ataatgacgt tgctcataaa attctcctcg tttttataca taaaactata
    12601 ccgcaactca cgacttttcg ttttctaaga gattgcgaga catagcattt ttttaaagtt
    12661 tatcatataa tagttaagta tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt
    12721 catggtacac taaataaaac tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa
    12781 aaaacacgct acaaagcggt gattgccaac caaacatata ctattatcgg acgggaaacc
    12841 aagcatcata tggatatcgt aacgaagctg atcaatgaac agttagcaga attgaaacaa
    12901 ttatcaccac aaatggataa tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg
    12961 gatcaattga agaaacaaga aagaatcttg gagctggaag aagaaacagc agaattaaag
    13021 aaaaaaatga tcaaatttac agagcttgaa aatcgtgtga aacgcattga agcaatcgag
    13081 aacgaagctc gggaagtatt gaaagaaaat ggtcaagcag atcataccat taaaaatcat
    13141 gtggaagcac aacagatttt aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct
    13201 aattaagatc cttttatcaa aataagaaaa atttaaaaaa agttagaaga gaaaggaacg
    13261 tagcatgctg agtttgctga ttttattttt attgctaatt gcgttttttt ctggagcacg
    13321 tcgcgggttt gctttgcaaa tcatattcgc gattggatat gtattgtcat ttattgctgc
    13381 ccagcatttt tacaaaccat tagcaagtca tttggaatta tacattccat acccagctgt
    13441 cacgccaaca tcaaagttag cattttttga tcaggtattt gcctttcacc tagatgaagc
    13501 tttttatgca ggaactgctt ttctgattat tttgctaatt ggctggctgc ttacccgatt
    13561 tgtgggggtt tttgtccatg gattgacgta tgtgccgatc ttgagacagg tagactggat
    13621 tgcaggaggc atcctgtcac taatcatggc ttacgttact atatttttga ttttgcagtt
    13681 acttgccttt gttccattag atgtggtcca aaatcaattc ggcgagcata gtttcgctcg
    13741 ctttatcgtt gagagaacgc catttcttac caacaagatg tatgagcttt gggtaacgaa
    13801 tattttgaat taaggaaaat agtgctggaa aatatttcgt tttttgtaca gagtagatta
    13861 tttaagaagg ctgcgtagcg tatgtccggc acagctcttt tttcatagtg aaagataagg
    13921 tgataaaatg aataaccgaa tccttgaaac attggaattt gaaaaagtca aacagatggt
    13981 cagacaattt gtagtgactg cacaaggaaa agaagaactg gcagaattgg taccagtgag
    14041 cgaggaacaa acgatcacaa actggcttca agaaactgag gatggattga aagtgcaacg
    14101 tctgcgaggc gggatcccta tacctaaact tgaaaatatc cgtccccata tgaaacggat
    14161 tgagatcgga gcagacttga atggagtgga gcttgcacaa gtttccagag tattgtctac
    14221 gacaagcgaa ttgaatcgtt tcattgatga tctttatgac agtgaaatcg aatttgcacg
    14281 tttgtatatg tgggcagatc agttagtcac gatccctgtt ttgagccgcc gtttaaagga
    14341 agcaatcgat gaagacggac gcgtgactga tgacgcttct cctgagttaa agagcataag
    14401 acagaacatt cggcgaagtg aacaagctgt tcgtgaacag ttagatggga tcgtccgtgg
    14461 gaaaaatgca aagtatttaa gtgatgcgat catcaccatg cgaaatgacc gctacgttat
    14521 cccagtcaaa caagaatacc gcggtgtatt tggcggtgta gtccacgatc aaagtgcttc
    14581 aggacagact ttatttattg aaccaaaaca agtggtggat ttgaataatc gtttgcgtca
    14641 ataccaaatc gctgaacgaa atgagattca acgtatttta agtgaacttt ctgctgaact
    14701 tgttccccat cgtcaagaaa tcatccacaa tgcttatgtg attggtaaaa tggatctgat
    14761 gaatgcaaaa gcacgatttg gtaaagaagt aaaagctatc gtgccgggaa tcagtgaaga
    14821 caatcatgtc gtcttgaaac aagcaagaca tccactgatc gatcaggaaa aagtagtttc
    14881 taatgacatc acaatcggaa aagactatca agccatcgtc attacaggac ctaacacagg
    14941 tgggaaaacg atcacgttga agactttagg tctgttacaa ctgatggggc aagcaggatt
    15001 accgattcct gcaggtgaag aaagtcaaat cggtattttt gaggaagtat ttgcggatat
    15061 aggagacgaa cagtcgattg agcaaagtct atcgaccttt tcttctcata tgacgaacac
    15121 tgtagatatt ttatcaaaag tcaatgaaaa aagtctggta ttatttgacg agctcggtgc
    15181 aggaacagat ccgcaagaag gtgctgctct agcaatcgca atcttagatg atcttggtaa
    15241 aaagtctgct tatgtgatgg caacaacgca ttatcctgaa ttgaaagttt atggatataa
    15301 tcgggcaaat acgattaatg ccagcatgga gtttgatgtg gatacattaa gtccaacgta
    15361 tcgtttgctg attggcgttc ctggacgaag caatgcgttt gaaatctcta gccgattagg
    15421 tcttgataca gaagttattg atgaagcgaa gcagttgatg aatgatgaga gccaagattt
    15481 aaacgaaatg atcacagatt tagagaatcg ccggaaaatg gcagagaccg aatatctgga
    15541 aatgcgtcat tttgtttctg aggcacaaga acttcacgat gatttgaaag aagcttacag
    15601 ctatttcttt gaagaacgtg aaaaagaaat ggaaaaagca aagaaaaaag caaatgaagt
    15661 cgtgtcagag gcggaagaaa aagcagaaaa aatcattgct gatattcgta aaatgcaaca
    15721 gcagattggg caaggaaatg tgaaagagca tcagttgatc gatgcaaaga cccaattagc
    15781 aaatctacat caagaagaaa ctttgaagaa aaataaagta ttgaagaagg cgaaagaaca
    15841 aaaaacgttg aaaccgggag acgaagtgtt ggttacgact tacggccaaa gagggacgct
    15901 tttacgtaaa aacgggaatc agtggcaagt agaaatcggt atattaaaaa tgaatgtgtc
    15961 cgaagacgag ctgacgcctg ttgctcctca aaaagagccg acacaacgag ttatccatgc
    16021 tgtacgatct gaatctagta gtcacgtgcc gaatcaattg gaccttcgag gaaaacgata
    16081 cgaagaagcg ctaagtgagg tagatcaata tttggattct gccattctag ctggttatcc
    16141 tcaagtgact attgtccacg gaaaaggaac aggcgcactt cgcaagggaa ttactgatta
    16201 tcttaaaaac catcggagcg tcaaaagctt tgagtttgca ccggcaaacc aaggcggaaa
    16261 tggtgcgaca attgttaaat ttaagtaaag cttactaaag gtaagcagat ggttcgctat
    16321 ccaagtatag aaatgctata ctagtcatag attttaaaaa tggaggtctt tgatatgtca
    16381 caagtaatta cagataagac atttaatgaa gaaacagata aaggacttgt attgattgat
    16441 ttttgggcaa cttggtgtgg tccatgtcgt atgcaagcac cgattttgga tcagttggaa
    16501 caagaatatg atgaagaaga attcagaatt gctaaaatgg atgtggatga aaatccagaa
    16561 actcctcaac aattcggtat catgagcatt ccaacattaa tgttgaaaaa agatggtcaa
    16621 gtagtagaaa aagctgttgg tgttcattca aaagaacaac tacgtcaaat gatcgatcaa
    16681 tatttataaa aacttacagc aaaaataagc tgagggtggg actgaatcca gttccgtcct
    16741 cggttttttt gcggttgagt taaggttatt caaattaata ttttttgagt ataattacgt
    16801 tgtcaggctg gattgttttt ttgacagaca tgctataata actttaattg aatatgaagg
    16861 ggagtaccag atgaacgaac gaatcaaaaa caaattagct ttacttcctg atcagcccgg
    16921 ctgttacttg atgaaagata aaaatggaac gatcatttat gtcggcaaag ccaaaatatt
    16981 aaaaaatcgt gtccgctcat attttactgg tagccataat acgaaaacgg aacggcttgt
    17041 tagtgaaatc gaagactttg aatacatcgt tacagaatcg aatatcgaag cattgctctt
    17101 agagatcaat ctgataaaga aaaatgatcc taaatacaac attatgttaa aggatgataa
    17161 aacctatcct ttccttaaga taacgaatga aaaatatccc cgtttggtga ttactcgtaa
    17221 ggtactaaaa gacaaagcac tgtattttgg accatatccc gatgtcggtg cggcgaatga
    17281 gacaaagaag atcctcgatc gattatttcc tctgagaaaa tgcaaaccta gtcagacgaa
    17341 ggagccttgt ctttactatc atttgggaca atgtctgtgt ccttattatt tcgacgtaga
    17401 tccgaaagtc tatacgaaaa tcgttgatga agtgaaaaac tttttaaacg gaggacacga
    17461 aaaaatcgag tcagagatcc atcagaaaat ggaaaaagct gctgaaaaca tggagtttga
    17521 aaaagctgca gaataccgag accagatccg tgccattgag acaatcatga ctcgccaaaa
    17581 aatgacaaat actgatttac tggatcgaga tgtatttggt tatgcgattg ataaaggatg
    17641 gatgtgtgtc caagtgtttt tcgtaagaca aggaaagcta attgaacgag atgtttcaat
    17701 gtttcctttc tataacgaag cagaagaaga tttcttgact tacctcggtc agttttatca
    17761 ggaaaatgag catttcattc ctaaagaagt cttgattcct gatacgatcg acaagcccag
    17821 tgtagaagct ttgttagcta cgaaagtgct gcaaccaaaa cgaggagaaa agaaaaaatt
    17881 agttgaattg gcgagtaaga atgcctcagt tgctttgaat gaacggtttg atctgattgc
    17941 cagaaaacag gaacggacga tcggagcagt tgaacgttta ggaaacgcga tgaatatccc
    18001 gaccccaata cgtatcgagt cttttgataa ctctaatatc atggggacga atcctgtggc
    18061 tgcgatggtc gtattcattg atggaaaacc ggcaagaaaa gaatatcgga aatataaaat
    18121 aaaaacagtc aacggaccgg atgattatgc atcaatgaga gaagtcatct atcgcagata
    18181 ttcgcgagtg attcgcgaag ggttgccttt acctgatttg atcttgatcg atggaggaaa
    18241 aggacaagta gatgtggcaa aagatgtctt agaaaatcaa ttaggaatcg atattcctat
    18301 tgctggcatg gcaaaaaacg ataaacataa aacaaatgag ttgctattcg gtacagattt
    18361 ggctgtagta ccgcttgagc gaaattcacc agagtttttc ttactccagc gaatccaaga
    18421 tgaagtccat cgattcgcaa tcactttcca tcggcagcta cgcagtaaaa acagttttgc
    18481 ttcccgatta gatgaaattg aagggttagg accaaaacga aaaaaaatgc tgcttaaaga
    18541 gttcaaatcg ttgaaaaata tcacagcagc cacgacagaa gaattacaag aaatcggact
    18601 gccgaaaaat gtcgcacaaa acgtatttga taaattacat caggaatgag gaaagggcac
    18661 tttacaaaga aagtaaagtg tcctatcatt ttatgtacaa caaaaagagc gtttacgttt
    18721 tgttggtaaa taataaatcg cttacgtctg gtgtctaagg atatatgtga tttcttgaag
    18781 aaggagtgaa gaaatgttag aagtcaagga tcttgttaaa acttttggga actacacagc
    18841 tgtcgatcat gtctctttcc aaatccccga tggaaaaatc atggggctaa ttggccaaaa
    18901 cggggctggg aagacaacga cttttcgttt gatcttggat tttttgacac aagaccaagg
    18961 agaagtgtta tggaatggcc atcatcttgg ggagaaagat tatgatgtca tcggttatct
    19021 tccggaggaa cggggacttt atccgaaagt aacgattcaa gaccaactgc tttattttgc
    19081 agaacttaga gggaaatcaa gaaaagagat tgaaccaaaa atcgactttt ggatggaaaa
    19141 atttcaggta aaagggaaaa agacggataa agtaaaatct ttgtctaaag gaaatcagca
    19201 aaaagtccag ctgatcgcca cgttaatcca tgaacctaaa ttgatcattt tggatgagcc
    19261 attcagcggg ttagatccgg taaatgctga attgctaaaa gatggaatat tggaattgaa
    19321 aaaacatggt tcatgtgtca ttttttctag ccacaatatg gataatgtag aaaaaatctg
    19381 cgatcatttg atcatgttgc gaaacggaaa aatggttttg aatggaaaag tacatgagat
    19441 tcgtgaatca tttggccgta cgaagttatt cttggaatct ggtttaagcc aacaggaagt
    19501 agaagaaatc gaaggcgtga aaaaagtgat ccttcatgaa gacaatacgt tagagattac
    19561 cttagaagat cctgaagctg ggaaagaaat ctttactcgt gcgactcagt tcggctacat
    19621 tcctatgttt aaccagcaac cgccaacttt ggaagagatt ttcaaattga aagcaggtga
    19681 aatcgatgcg taagttttgg attattacca aagatgtcta tttaaaaaat gttaagtcga
    19741 tttctttctt gatcatgata ttagtccctt ttatcatgct gggtatcatt tatgtagcag
    19801 gaaactttgc tcaacaaaat agtgatgtca ataaaatcgg cgtattggct gataatgagc
    19861 aaatcagtca gcaaatgagc caagtcaaaa cagatgattt tgagttcaaa gcagttgatt
    19921 ctgaagaaaa agcacaaaaa gagctttcgg aagaaaaaat cgatgcgtat ttagtagtct
    19981 caactgagaa tgataagata aatgggatgc tttatagcga aaattcatta ggacaaagta
    20041 cacaactact gatacaacaa caactgactg gcttgcaatc gatgcttcga gcgagtcaat
    20101 taggcatttc accagaagaa gtagctagct tgagccaaca agcaagtttg actcgacaaa
    20161 aagtcaattt tgattcaaaa ggaaaaatga cagtcggaga agataactcg acagtccaat
    20221 acgtcgtaag ttatgtagca acgattgtct tgttcattat tattttgacg tatgcgcaga
    20281 tcattgcaca agagatcgct tctgaaaaag gcacgcggat catggaagtg atcctgtcca
    20341 gtacgactgc acaaaagcat ttttacggaa aattaacagg tgttttactt gttgcattaa
    20401 cgcaaatggc attatacggt attatttttg gtataggata cagtcaattc aaagatatgg
    20461 aaatggtcaa aaatatatta gacggtatct cattggataa cttgattgga ccattcttgt
    20521 ggtattctct tctattcatg atctttggca ttttgatttt ttctgtttta gcagctttat
    20581 gtggctcttt ggtcaacaaa gcagaagata cagcaaaagc aattttgcca gtgacttatc
    20641 tatctctagg cggatacatg cttggattga tactaggagc ttcagatcca aacaatatcg
    20701 ttattcggat cacttcgtat attccattct tatcttcata tattatgccg gttcgcttag
    20761 caaatgagac agtcggtatg ggtggagcat tgatttcctt gttggtactt gtagccgtaa
    20821 cattcttatt gatgatcttg tctgcaaaca tgtacaaatc gaatgtactc gtttatagcg
    20881 aaggcggatt atggagttca ttgaaacaat cgatttcaat tatgaggaat gaacgaaaaa
    20941 aagcataaaa caatcctaaa atacagaagg aagctggaca tctattgttc ccagcttctt
    21001 tttgtataga cctattttat ttattttact atattggata tatattcatt gaaaatagtg
    21061 tataacaact ttataatatt gagtatacca atttttggag gcgttttaat ggaccgttat
    21121 ttagatttac tttcagaaaa attcccctca acggaggatg tcatcacgga aatcatcaat
    21181 ctcgaagcaa ttttgcagct tccaaaagga acagagttat ttttgagtga tatacatggc
    21241 gaatttcctg cttttgatca tattctgcgt atcggttcag gaaatcttaa agaaaagatc
    21301 aaagaccttt tttctgaaca aatgacggaa acggatatca gccaattcac tatttttacg
    21361 gcttatccag aatatgcatt aacgactgat tggtacaaag aacaagataa atcgcagctg
    21421 atccatcatt tgatcgatct tttacggttt acgacagtca aatatacccg gtcaaaagtt
    21481 cgaaaaggat tgccgaaaga atatagctat atcattgagg aactcttgta tatcgatgac
    21541 cgtatcaatg gaaaaaaaga ctatgtaaaa aaaatcattg aacaacttgt tttgatgaag
    21601 gaagaagaaa aatttttaac gaaacttgca cagacgatcc aaaaatctgt catcgatcat
    21661 ttgcatatcg ttggagatat ttttgaccga ggaactcaag cagccaaagt gatggatcga
    21721 atcatggatt tttcttctgt agatatccaa tggggaaatc atgatatttt gtggattggt
    21781 gcgtattgcg gctctgaggc atgtttactg acacttttac ggattgcagc acgctataat
    21841 tatttatttg aattagagaa agaatatggg ttgaatttac gtccattgtt ttcttttgct
    21901 catcaaactt atcagaagaa tccagtattt gcgcctaaaa acagtaaaaa tctatccgaa
    21961 agagagcaag aagaattaga aaaaatccat caggcacttt ctatccttca attcaaatca
    22021 gaacatcagt tattggatcg tcgacctgaa tttaaaatgg acgatcgtaa attgctggaa
    22081 aaaatcgact atgaagcatc aacgattgat ttagaaggtc agctctatga gttgaaagga
    22141 acttgttttc aaacgatcca gccagatgat ccaaaaaaat tcttggaaga ggaacaaaaa
    22201 gtgattgata gtttgatgta ttcattccag cactctttga gaatgcaaaa acatatgacg
    22261 tttttgatcg aaaaaggggg aatgtatctc acgaacaatc agcatgtgct gtttcatggc
    22321 tgtatacctg tagacgagaa gggacagctc ttagcatttg aattagacca atcttatcgc
    22381 ggaaaagaac tgctgggctt ttttgaaaaa cagataactg aaagcgcaaa agatctgact
    22441 gcaaaagaag atttagcaac cgatttgatt tggtatgcgt ggagtgggcc attttcgcct
    22501 ttattcggca agagtaaaat ggcgactttt gaacgttatt ttctcacaga gcctcatacg
    22561 cacgtcgaga aaagcaatcc ttattatcac ttgcgagatg aagagtggtt cagtgaaaaa
    22621 attttagcag aattcgacac aaaagaagaa gggtcagcaa tcattaatgg gcacacgcca
    22681 gtaaaagtaa aaaaagggga gtcaccgatc aaagcaaatg gaaaagtatt tgtcattgac
    22741 ggaggtttat ccaaagctta tcaaaaaacg acaggaattg ccggttattc actattatgc
    22801 aattcttatg gtttccaaat cgtgacacat tatccgtttt tgcctattga acaacaattt
    22861 gcaaaaaaat ccgaccaaac aaatgtgaaa aaagtaatcg aacagcaact accacgcaaa
    22921 ctcaatcgtg atacaacaaa aggtatggaa ctgcagcagc agattaccca gctcaaaagg
    22981 ctgttggact atcgaaagga cgattgagaa gaattccagt gaacattgtt ccatagactg
    23041 gagactgaga caaaagtcgt gtcgcagtct ctttttccgg ataaacggtg ttcttctgcc
    23101 aactcctcgg tcacaagtca cactattcaa caaacatgaa aagctgtttt ttgaaattgc
    23161 tccttgtgac tcagagttga acggcaggct cacgacctct tggtataaac ggtgttcaaa
    23221 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tgaaaagcca ttttcacaaa
    23281 atttttctta atactcgaaa ctaaacagct ttttcacaac cccctcagaa agtctattaa
    23341 aattcattca caaagaaatc gaataagcgc tgttcagatg gatgtacacg gtgggtaagt
    23401 tccggatgcc attgaacacc gaggatttta tgcatatcat ctttgctttg aaccgcttca
    23461 atgagtccat cgttagaaaa agcgatggct tttaataaag gtgaaagatc tttgactgcc
    23521 tgatgatggt aagaattcac aaaatatgga tcggggagaa ggttgcttaa caaactgtct
    23581 gaaacaatct tgacttcgtg agtcgtaaat tgcggggcag tgggctgttg ttcatgtttt
    23641 actgaccact ttggatataa tgaaagatct tgataaagtg taccttccaa aacgacattc
    23701 aatagttgga gtccccggca gactgcaaaa attggttttc tttgtttcaa tgcttccaag
    23761 atcagtgcct gttcaaattg gtctcgttga atatttgttt ccgttagctt tggatgagga
    23821 tcttccatgt aaagttgtgg actgacatct tgaccacctg caagaataag tttgtcgatt
    23881 tgtccaatca gttgaggagc tgaatctgga gacatgactg gcagaataag aggcaatcct
    23941 tgagcatcta aaactgcatc cacgaatcct tgcggtgtat agctcacttg atttccttgg
    24001 aaagtatctg ttgctcgaat cagttgattt ccggcaatgc cgatgattgg tttcatttgt
    24061 attcccccta aagtagactt attgtaacaa ggatttaaaa aaaattaaac tatgtaagac
    24121 ttgcccctat acttatctgt tcttgctgtg ttactatctg taaagagaaa tttcgtgtga
    24181 aattcatgac gatagttgaa aatattgctt aatttgttat aataaaaagt aagcagaaat
    24241 aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa ggggtgagaa ggacatttta
    24301 ttgaaggggc ctgcctatac taagttatct ttagaggtac atactttcaa tgaaaaaaga
    24361 aagcatgtac ctctttttta aatcgaaagg aagtgaagca agtgttagaa gctttggaac
    24421 aaatccgaaa agcagaagaa gaaaatgaaa agcgtgctca aacactgcgg cagcagctga
    24481 agatctatgc aacagaaaaa gagcaggaat tggaaacgat ccaaacaaac tatcgacaac
    24541 aactaaaaca gttggtggaa gaaaaagaac aatttgaaat acaagaatta gaaaaagaaa
    24601 aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac tttgaaagat caatatcaaa
    24661 agcatcagag tcaagcgatc ggtgcgatca ttgaaagggt gaaagaaact tatggccgtc
    24721 actaagatgg aaaaagtgac cctcatttca gataaaagaa atcaggagag gattttacaa
    24781 gcagtacaag gaatccaaaa cgttgaaatc cgggatctgt atcaaaatag aacaaacaac
    24841 cagtgggtgg agaagtattt tccagatacc acgattattg ataaagaagc aaaattaagc
    24901 gctttaggta atcggttgaa agacatccaa gaagctatcc aattcataca acaccacggc
    24961 gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac tttccttaca agatttggaa
    25021 gccaattatt ccgaagaaaa tttcataaat aaattgaaag aagtccttga tttaaaagaa
    25081 aagtgggaac aactaactga agaacgtgat cgtttagaag aagaggaaga atggcttctt
    25141 cattggcaac agctggatat tgctccaagt atgtatgcaa gtaagacgac catttttgag
    25201 atggggactg tgagtgcagc aaattttgaa agtttcaagg aagaactgag taagcttgat
    25261 gacgtttata tggaagaaat agattatacg ccaaaacaag tttatctttc gtatgttgct
    25321 ttaaaaaaat cagttgctgt tatagaagaa attgctggga aatacgggtt caataaagaa
    25381 acgtatcctt atgaacaaaa tcctaaagaa aaactgcaag agaccaagca gcaattatcc
    25441 attgttcacg agcagcaaaa gaagataacg acagcgttag gtcagtgcag tggctatatc
    25501 caagaatttg aatgggtaga agaagtcaca cttgccgttg cagaacgaga aaaaataaaa
    25561 gaacggttta tccatgcgac ttatctgatc gttttgcaag gttgggtaga tgcagaagaa
    25621 aaacagaatt tgctgcatgt gttaaacgaa accgtatcag aagaagatat atacgtcagt
    25681 ttcgaaacac caaactcaga agagattcag caggaagtcc ctactaaatt gaaaaaccat
    25741 ccgcttgtcg aaccttttga attgctgact gaaatgtaca gtctgccaaa atatgaagaa
    25801 gtagatccaa caccttggat gacaccattt tatcttgtct tcttcgggat gatggtcgca
    25861 gatgttggtt atggtttgct gatgctcatt gggacaatac tagctcaaaa actacttgtt
    25921 ctaccacgcg ggatgaaacg tttcgtaaaa ttctttgaga ttctctcgat accatcgatc
    25981 atctggggat tgatctacag ttcatttttc ggacagtcat taccaaaaga actgtttggc
    26041 atccgcttgc ctttcccaat cctatctacg acggatgacg tcaatacgat tttgatttta
    26101 tccgtgattt tcggtctgat acagattttg gttggattat ttgtggcagc aaaggaaaat
    26161 atcaggagaa aagattactt gagtgctgtc agtgatggat ttgcctggca agggatccta
    26221 attgggatcg ttattgcgtt ggttggtgcc atgttgataa aaaatacagc atttgtttac
    26281 cttggcgctg ggctatcgat cattgctgcc ttatgtatct tgatcgttcc gattatccaa
    26341 acgccgtcta aagtcaaagg tgctgccaaa ggtgcgtata atctttacgg actgaccggc
    26401 tatatcggtg atttagtcag ttatacacgt ttgatggcat tgggtatttc aggcggaagt
    26461 attggagcgg cattcaatat gctagttgct ttcatgccgc cagctgctcg attcagtgta
    26521 gggatccttt tgatcatcgc tttacacgca ctgaatatgt ttttgacact gctaagtgcc
    26581 tatgtccatg gcgcacgatt gcagtatgtc gaattttttg ggaagtttta tacaggaggc
    26641 ggacgtgcct ttgatccgct gaaaacggcg gaaaaatacg tcaacatcaa tcacaagaaa
    26701 aaaaacaata aataattgga ggatttttat ataatgatgg attacttaat tagtcaaaat
    26761 ggtggaatgg tatttgcggt attagcgatg gcaacagcga cgattttttc agggatcggt
    26821 tcagctaaag gtgttggaat gacaggtgag gcagcagcag cattgacaac aagtcaaccc
    26881 gaaaaattcg ggcaggctct gattctgcaa ttgcttccag gtacgcaagg tttgtatgga
    26941 ttcgttattg cctttttgat ttttatcaac ttaggaaatg acatgtcagt tgtacaagga
    27001 ctgaactttc taggtgcttc tctgccgatt gcttttacag gcttgttttc aggtattgcc
    27061 caaggaaaag tcgcagcggc aggtattcaa attttagcga aaaaacctga acatgctaca
    27121 aaaggaatca tttttgctgc gatggttgaa acatacgcga ttttaggttt cgttatttcc
    27181 ttcctattag tgttgaatgc ctaagcataa aggaggaaaa gcagatgaac gccattgaaa
    27241 aaatcatctc tcagatgaac gaagcagcag agcaggaaag agcggcgtta gaacaagaag
    27301 agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac gcaagttgaa acagaacatc
    27361 aaaaacaaaa agaaaaacaa atcgaattac tcgaaaaaaa ataccgtcag ctaaggaatc
    27421 gccagcaagt agaagtacga caagaaaatt tgaatgcgaa gcaggaattt ttaagacgat
    27481 tgtttgcaga tgcggtaact gaaatggaaa attgggatga atctgaacaa atccagttta
    27541 tcaaaaatgc attgtattca ttacctttga caggaaaagt agcattcatt gctggagaaa
    27601 aatcagcagc ctatctttct cagacgttgt tggatgaatg gaacaatgaa cttcccttta
    27661 tgatggtttt aagtgatgaa actgttgctg atcaagcggg ttttctgata aacgatcaag
    27721 gagttcagta taattttctt ttcagcagcc ttgtacaaga tatccaagga acgatgagtt
    27781 ttgagattgc caatcaatta ttcgaataag gagggatcag tgtatggaat accacgaact
    27841 aaatcccttg attcgcggaa gagagcttga acttttgtcc aatgagacat ttgaacggat
    27901 gattcaaaca aattctgtcg aagcacttgg tgaaatattg aaatccacga tttacagtcc
    27961 atatatttat gacggatatg aatatgactt cgaacagaat cttttaaagg agcagagccg
    28021 tctatttcgc tggttaaagg aatcagcacc ggagcctgaa atcgtttgga tctatacgat
    28081 gcgttatact ttccataatc tgaaggtttt gacaaaagct gagatgacaa atcaaaatct
    28141 ggaccatttg tatatcgacg atggatttta ttcattagaa accttgaagg atgccattca
    28201 tacgcaagtt tcaaatgaac ttcctgaaag cattatggac agtatacgag aagtacgtga
    28261 ttattttgaa gagtctacca ttcttcaggg aattgacgtg atttatgatc gacagttttt
    28321 gacagagcag cgacggcttg ccgataagct aggatatcaa gaattgctga cagaagtcat
    28381 tgcttttatt gacttgacca atatcacaac gatggctcga ggaatattgc agaaaagaac
    28441 atatgggttc atgacgacgg tactttccag ctcgggaagt atcgaaaaag aagtattttt
    28501 gaactttgta gataaagata tggaagatta cacgaatttt ctattagaga cagattatgc
    28561 tgattttctg cgccctgtca tccgtgaggg aaatattgat ctcgtaaagc ttgaacagtt
    28621 gaaagatgat tatctaagtt cattgtatca agaagcacaa acccaggctt tcggaccact
    28681 gccactacta gcctttttga acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt
    28741 tatcggcaaa agaagccatt tcgaatcaga aacaatcaga aagcgggtga gacaagtcta
    28801 tgacgcataa gatcggtgta gttggcgaca aagattctgt tttgccattc aaattgtttg
    28861 gctttgatgt tcgttatggt acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg
    28921 caaagaatga gtatggcgtg atttatgtca cagagcaatg tgcagcattg agcccagaaa
    28981 cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt attgattccc aatcatcaag
    29041 gatcattagg gatagggctg gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa
    29101 atatcttata gtatgaaaag gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct
    29161 ccggacctct tgtaatggct gagaacatgt ctgatgccag tatccaagac atgtgtctag
    29221 tgggagatct aggagttatc ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc
    29281 aagtatatga agagacttct ggaattggtc caggcgaacc ggttcgctct actggtgaag
    29341 ctttatctgt tgaattagga ccagggatca tttcacaaat gtttgacgga atccaacggc
    29401 cattagatac ctttatggaa atcacacaaa gtaatttctt agggcgcgga gttcaattgc
    29461 ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg
    29521 tgagtgctgg agatgttatc gggatcgtag aagaaacaaa agttataaaa cataaaatca
    29581 tggtgccaaa tgggataaaa ggaacgatca aaacaatcga aaaaggttca ttcactattg
    29641 atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa
    29701 aatggccagt aagacgtagt cggccaatca aagaaaaatt aaacccggat gcacccatga
    29761 tcactggaca gcgagtgatt gatacattct tcccagtgac aaaaggagga gcagcagctg
    29821 tacctggtcc ttttggtgca gggaaaacag tcgttcagca tcaaattgca aaatgggccg
    29881 atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt
    29941 taaatgaatt ccctgaattg attgacccta atacaggaga atcgttgatg gaacgtacgg
    30001 tattgattgc taatacatca aacatgccag tagcagctcg agaagcttct atctatacgg
    30061 gaatcacaat tgcggaatat ttccgtgaca tgggttatga cgttgctatc atggcagact
    30121 ccacttcacg atgggcagaa gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg
    30181 gtgatgaagg ttatccagct tatcttggtt cacggcttgc ggaatactat gaacgatctg
    30241 gaagagtcat tgcattagga tcagaaggtc gcgaaggaag tataaccgca atcagtgctg
    30301 tatccccgtc tggaggggat atttcggaac cagtcacaca aaatacattg cgggtcgtaa
    30361 aagtattctg gggtcttgat tccagtttag cccaaaaaag acattttcct tcgatcgatt
    30421 ggatacaaag ctattctctt tattcaacag aagtagggaa gtatatggat catatccttc
    30481 aagctgactg ggcatccatg gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc
    30541 tgaatgaaat cgtgcgtttg gtagggattg attccctttc agataatgac cgtctgacat
    30601 tagaagtggc aaaatccatt cgtgaagatt atttgcagca aaatgctttt gatgatgtcg
    30661 atacattcac ttcaagagag aaacaattca aaatgttgaa gatgatcttg acctttggtg
    30721 aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag tgagatcatg gacggtaccg
    30781 tatcgattcg tgaaaggatc ggacgaagca aatatatccc ggaaacagaa ttagaaaaac
    30841 tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca
    30901 tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt
    30961 gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag tccgtatgca aaatggcgag
    31021 attcgtcgtg gacaagtatt ggaagtccaa gaagataaag caatggtcca aatattcgaa
    31081 ggaacaagtg gtatcaattt acgtgactct tctgtacgat ttttaggcca tcctttagaa
    31141 ctaggtgtat cggaagacat gatcggtcga gtattcgatg gtttgggacg accgaaagat
    31201 aacggaccag agatcctgcc tgaaaagcat ttggatatta acggagaagt aatcaatcca
    31261 gtagctcggg attatccaga tgagtttatc caaacaggta tctctgcaat cgatcatttg
    31321 aatacattag ttcgtgggca aaaattacca gtattttctg gctctggtct tcctcataaa
    31381 gaattagctg ctcagatcgc gcgacaagca acggtactcg attcatcaga tgatttcgcc
    31441 gtagtctttg ctgctattgg aatcactttt gaagaagctg aatttttcat ggaagatttt
    31501 cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga atttagcaaa cgaccctgca
    31561 atcgaacgta tcgctactcc tcgtatggct ttgactgctg cagaatatct tgcttatgaa
    31621 aagggaatgc atgttttggt tatcatgaca gacatgacca actatgcaga ggcattgcga
    31681 gagatttctg cagcacggcg agaagttcca ggaagaagag gatatcctgg gtatctttac
    31741 acgaacttag ctactttatt cgaacgagca ggaagaatcc gcggactaaa aggatcggtt
    31801 actcaaattc cgattctaac aatgccagaa gacgataaaa ctcatccaat ccctgacttg
    31861 actggttata ttacagaagg acagatcatc ttgactagag agctttacaa gagtgggatt
    31921 cagccaccaa ttgatgtact cccgtctctt tcgcggttaa aagataaggg aactggaccc
    31981 ggaaagacaa gagaagacca tgcagctacg atgaatcagc tttttgcagc ctatgcacaa
    32041 ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat ctgctttatc tgatgtagac
    32101 aagatctatg cgaagtttgc tgagcggttt gaaaaagaat atgtcaatca aggattctac
    32161 acgaaccgtt cgattactga aacacttgat ctagggtggg aactgctatc gatgttgcca
    32221 agaaccgaat taaaacgaat caaagacgac atgctggaca aatatctgcc agaagggaag
    32281 tgagttctga tggcacgatt gaatgtgaat ccgacccgca tggaattgac tcgattgaaa
    32341 aaacagttga caactgccac aagagggcat aaattattaa aagataagca agatgaactg
    32401 atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc tgcgtcaaga aatggaagag
    32461 tctatgacag aagcaatgtc tgattttgta ttggcaaatg cttcgatcac ggaacctttc
    32521 atcgaagaat tatttgtttt gccggcggaa aacgttgatt tgtctatcgt agacaaaaat
    32581 atcatgagtg taaaagtacc tgtgatgaat tttgaatatg atgaacagct gacagacaca
    32641 cctttggaat acgggtattt gaattccagt gccgaactgg atcggtcaat cgatcggttt
    32701 actaccctgc ttccaaaact tttggaactg acagaaatcg aaaaaacttg tcaactgatg
    32761 gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat tagaatatat gacgatccct
    32821 caattagaag aaacgatcta ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa
    32881 gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg actaagtggt atcttttttt
    32941 aaaagaggaa aacgatgatt aaacaaagca aacaaataaa gaaacgtttg ccggctgttc
    33001 agcttctcgc attaggattt tttctcctga ttttagttgg cggcggccta cttaccctac
    33061 cattttttag taatagtggg catgggacaa attttatcga tgcgcttttc actgcaactt
    33121 ctgctgtctg tgtaacggga ttgacaacat tgaatacagc agagcattgg aacagtgctg
    33181 gtcaattttt gatcatgctt ttgattgaga tcggcggtct tggttttatg atggttccga
    33241 ttctatgctt tgcattgata aagaagaaag tcagtctcag cacacgtatt atattaaaag
    33301 aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct catgctttac atcctaaaat
    33361 tggcctttgt gatccaattg attggtacgg ttgctttatc ttttgtattt gtgcctgaat
    33421 ttggctggtt gaaaggtatc tggtacagtt tgttccatgc aatctctagt ttttgtaatg
    33481 cagggtttga tttgttagga gacagtttag ccaatcacca gcaaaatgtt tatttgatca
    33541 tggtggtatc tgctttgatc atttctggtg ggttaggctt catcgtatgg cgtgatttac
    33601 tggaatatca tcagaaaaag aagatgacga ttcattcaaa aatcgcatta accgttacag
    33661 gtaccctttt attaggcgga ttcgttgtgt tctttttcac agagcgcaat ggggcaacgc
    33721 tagtggaagg gaatatgttt caacgattgg caaacacctt ttttatgagt gtgacaccta
    33781 gaacggctgg atattattca gttgattacc tccatatgag ccatgcaggt ctgattttaa
    33841 cgatgttttt gatgtacatc ggcggaacat ctgggtcgac ggccggcgga ttgaaaacaa
    33901 ctacgttagg tgtgttgctg atccagatgc gttcgatgtt gaaaggacgt acgagagcgg
    33961 aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg ggcacttact ttattttttg
    34021 ttacgttgac gttgtgtgta gtcgccatca tgattttatc ggtgactgaa acgataccta
    34081 aaacttctgg catcgaatat attgcttttg aggtcttctc tgcgtttggt acagtaggtc
    34141 ttacgatggg attgacgcca gacttgactg caatagggaa attgatcatt atagcgctta
    34201 tgtacattgg acgagttggt gtattgactg tggtcttctc tctgatgaca aaagcacaaa
    34261 gaacacaatc aaaatacaaa tatccagaag aaagtgtcat gattggataa tttcatcttc
    34321 tgacagaaac ctagtgattt agtaaaataa gaaaataaaa taatttttct tgcttcttgg
    34381 atactttaag tgcaaaatgt tcgtttattt tttgaggcac tacccgttgt tgaacctcgg
    34441 gtagtgcctc ttttttattg aaaggaatca ataagatttg attcctttat ttatcaatag
    34501 gttataattc atattttttt attttttctt ttacgatact tagtccagaa gctaaaattt
    34561 caaggttatt agaggatagg gaaattcgaa cgaatgcatc tggttgcgta cgtgtagtaa
    34621 agcgtctgga atgataaagt cggataccgt tagcaagaaa gtcattctca attatcttag
    34681 ggtcaatctc aggatcaact ggaagaattc gatagtaagg gacacgattc gttggttttt
    34741 ctactttgaa aagagaatca aaaagctgat tggctctttt tgtaagactt aatttagttt
    34801 ctaagatcct atttgcttca tttgaatgga ggatttgagt gatgacttcc acatctaatc
    34861 cagaagtttt cacatttata ttaaaaattg cttgttctat cttgctttgg acatgtactg
    34921 gaactacaag ataagcaact cgcaatccag aacaaataaa ttttgtcatt ccagctaagt
    34981 agattgtttg gttaggtaat aaattttgga aaggaggaat gatcgtatgt tgatagtaag
    35041 ttgttaaaaa tgaatggata tcatcttcaa taacccatag accttctttt tcaatcactt
    35101 cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc tataggatta ttacaagaag
    35161 gcataagaaa gacaccatga atcttctttt ttctacattc ctgaatcaat atatcaggtc
    35221 gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa atgatataac tgagccaatt
    35281 caatgaaatt agcgtatgta tatcggtctg ttgctattct atcacctgga gagaaaagtg
    35341 cagaaagtgt gatagccaat ccattttgaa caccggaaac aatagataga gattctaagg
    35401 acggaatgac tccttgagtt tttaaccaat ttgctgcagt agtcaattga aaatttgtgc
    35461 ctttgggatc ttggtaatta agcaaaccaa caagatttgg gttattacta gttgattgaa
    35521 taaacgggac aacgaattta ttacattctt caaaagaact aaccagacca aaatcaatga
    35581 tatgttcggt tacttcattg gtagagatgg tgatggactc aaagacattg ggagaaataa
    35641 atgtgccact tccaatattc gtatacagca aacctttctc aattccatat ttataagcct
    35701 tgccaacggt agtaaaattt aaatctaaat aatcagctag ctctctttgg gagggtaact
    35761 gagtgttttt ctgtaattta ttctttaaaa tatcttgttc taattgttga atcaacgatc
    35821 gataataagg acgcttcaat ttttgtttgt ctggtcgcca agttaacggg taattgtcat
    35881 aagaattaat aggcattttc tccactcctt aaaattgtat accatacaat tttaccattg
    35941 attgtattga ttttagaagt taatcttttg tgaggaggga attgtctatg aaaattatta
    36001 attttgagga agtagtagct tcttttagtt ttgaagaagc gattcaagta atgaaaaaat
    36061 gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc tcgattggtg gaaattttac
    36121 ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc ttatttgggg gagaacagat
    36181 attttggcac aaaagtcatt accgcttttc cggataatca tcagtatcat ttaccttctc
    36241 atttaggaga gattttcttg tttgatgctc aaaacggact acccaaagca ttgataaacg
    36301 caaatgcagt cacctggatt cgaacagctg ctgtatctgc gctggcaaca gattacttag
    36361 caaatccgca tgcagaaaca cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt
    36421 tgactgcgat ttctaccatt cgtcccatca ctacagtgtt tgtttacgat attttagaag
    36481 aaagacgaaa tacttttatc gctaaagcta aaaaacagta tcctaaaata acgtttataa
    36541 atgcaatatc agtagaagat gcagtacgtc cagcggaaat catttgtacg ttgacctcta
    36601 gtaaagaggc atttcttcaa aaagagtggg taagggaaga cgcacatatc aatgctgtgg
    36661 gcacatttac tcctgataca agagagataa gtacatcgtt gatggaaact tctcaaatct
    36721 atgtagatga ttactcagct gccttaacag aaagcgggga cctgcttata gccatggcag
    36781 aaggagattt agaggaatct tgtattgttg gtacattagg cgaattagtt tctgagaaaa
    36841 aaacatttta taaaggagaa tcgaggccga cgattttcga cgcagttggt ctagctattg
    36901 aagatctttg ttgtgcagaa tatatttata acaaattaga gaaaggggct ttaaaatgaa
    36961 cttagattat tacgtagttg atgcatttgc agatgaggtg ttcaaaggaa atccagcagc
    37021 ggtttatgtg ctagaagaat ggctgccaga aggtacgatg caaaaaattg ccattgaaaa
    37081 caatttatct gaaacagcat ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt
    37141 tacgccagat cgagaaatcg atctttgtgg tcacgcgaca ttagcaacag catttgtttt
    37201 atttaactat tacaaaatac ctgatgaaac gattaaattt tctactcaaa gcggtaatct
    37261 atttgtgaca agaattaaag attattatta catggatttt cctagtatta tgccgaaaaa
    37321 agtgccgata ttgtctgaat atgaagaagc gattggggct aagattaaag aagcctactt
    37381 agcacgtgat ttatttttcg tattagaaga tgaaaaaacg gtggctaaat tgcagccaga
    37441 ttttacagct atcaaaaatt ttgaattagg aataggagtc atcgtgacag cagaaagtgt
    37501 gcaaaaagac tttgtttcta gaaccttctt tccaaaatta acaatcaatg aagatccagt
    37561 atgtggatca gcacattcta atcttattcc ttattgggca gaaagaatga ataaaacaaa
    37621 attgtcagct tatcaaatgt catcaagggg cggggatcta gaatgtgaat taaaagaaga
    37681 tcgtgtgata ataggaggaa aagcagtttt gttttctaaa ggtacctcgt ttatttgatt
    37741 tctttcaaat gaattaaaaa taaattggtg gagagtggca accttatagc tacaatggtt
    37801 gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt ttgcgtctca ttttaattat
    37861 tggtgtttgt tttttattca atcaagagta aaatgcccca tactaactat cctcggatga
    37921 cttcgatttt ataaccatca gggtcaatga caaagtagta agaaggcgca gtgccaggta
    37981 atccttttaa gtcagtaatc tcaagaccag cagctttatg ttgctcgtgt aatttttcta
    38041 gatcatcagc tgctatagcg atatgaccat agccgtctcc tagatcatac gcaccatgat
    38101 catagttgta agtcaactct aattcatagt catccccagg aagtgtcaaa taaacaagtg
    38161 taaatttgtt ttctggaaaa tcacggcggc ggctttcttc aaaaccaaat gccttttgat
    38221 aaaattcgat tgatgcttca agatctttca ctcgaacaca tgtgtgagcc attttcatag
    38281 attcatctct ccttttcctt ttactcaatc ataacaaaaa gaagtaagga aatagttgat
    38341 ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata gaaaataaaa ctgttaaggt
    38401 ggaaactatt catggataaa ccaatttttg gtgaaaaaaa gcaatttgct gattatcaaa
    38461 cccgttatgc agcatatatt gtcatagaaa gagatcagca gattgcagta atcgaggctc
    38521 caaatggtgc tttttttctt cctggcgggg aaattgaagg agaagagacg aaagaagaag
    38581 cgattgtccg tgagctgata gaagaaatgg gaattgtggc agaaatttct tcttatctgg
    38641 gagaagctga tgagtatttt tactcgaatt atcgtgcaac ttattattat aatccaggat
    38701 atttctttgt tgctgattct tggaagcgta taggagaacc aactgaaaag accaataaaa
    38761 tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa acgtggtagc caccgatggg
    38821 cagtcgagag atggatcgaa aaaaataaaa actgaaagaa agcgagtaga tcagctttct
    38881 ctcagttttt ttatttatta cagtacatat tttttgattg cttcagctac accgtgctcg
    38941 tcgtttgatg aagtgatgac atttgctgct tctttgacaa gagaaacggc atttcccatt
    39001 gctactccta gaccagcata ttcaatcatg gataagtcgt tctcgttatc tccgatagcc
    39061 atgatttcat cttgatcaat accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta
    39121 cttgcttctt tatttagtac ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta
    39181 tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc
    39241 atcatcattt tgacgatgtt catgtctggt gtcatttctt caggtgttcg atattttagc
    39301 ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc tgatatctcg attaggcgtg
    39361 tagatcgctt tgtcatcgat cgaatggaga tgactgccga cacggcgtgc catcacttcg
    39421 atctctagga aatcttcatg tgtcagtccg tgacgggcga tgattttacc tgatttcgtt
    39481 tgttgtacga gtgccccgtt ataagtaata acgtaatcat tatcttgaaa aaggttcaac
    39541 tcgtttaact gttcctggac acctggtaag ggacggccag tgcataaaac gatcgaaaca
    39601 ccttttgcaa cagcagcggc aatcgcttct tttacttctt ttgttacttc tcttttctca
    39661 tttaataaag taccatcgat gtcgatagcg actagtttga tagacataat ttcctccaat
    39721 atattttaat ttatttcaat caatgcgcca ttacggatat ggctggcaaa ttcttgataa
    39781 gtttcgtcaa ataaatcata atgatcacgc aaggacggat caaccatttc ttttggaaag
    39841 aaaaatcgct catctccttg ttcttgtccc gctaatgcag caacaagctg actaacctgt
    39901 gataattcta tcaaagttcc gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct
    39961 tgttctgggc gataaaaatc ataaggtaga tcgtagctag aattcacagc agtgtaataa
    40021 acaggattat atccgacctt ttctactaac aaagtgagtt cttggactaa ttttgaatcc
    40081 cggttgccag aaaaagtagc agatttcagt ggtttacgat tcaaaaaacg tttagctaaa
    40141 tcatttaaaa taggatcagc agattcagac cattgagtaa agtaagtgcc caagacccca
    40201 tcatcaagtt tgaggtattc ttctaaagta aagcttccgt caagaaatgg cagaagtaat
    40261 tgagaatgga gttcaaaact gctggcatct tcttgataca actcgtgtgc gcgatgaagc
    40321 agatgctcca aaattacttc cataccacgg gaaacaggat ggaagtacac ttgcacatac
    40381 atttgatatc ggctaacgat ataatcttcc actgcatgca tgccactcat ggaaaaagcg
    40441 atcccgcctt cataaggacg aatcacacgt aatattcggg taagatcaaa tgttccatat
    40501 tctgttccag tgaaataagc atcccgaagc aaatagtcca ttcgatctgc atcgatctga
    40561 ctagaaatca tttggacaac ttgtgggttg gggtaagttt tttggatcac actagctacc
    40621 ttttcaggaa aaccttcttc tactcggtta agaatctgat acacttctgt ttctggagaa
    40681 gtgatgatct ctacagtgat ggcttcatga ttcgtatgga agatatgttc aaatgtatga
    40741 gagtaaggac catgaccgac atcatgcaac agtgccgcac ataaagtcac cagccgttcc
    40801 tgatcatccc agcctccgtt gcctattttt tcttttgaaa aatttcgaga aaagatatcg
    40861 cagattcgtc ttgaaatctc ataaacgcct aatgaatgag taaaacggct atgttctgct
    40921 ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc gtcgtagacg ttggacttct
    40981 ttcgaattga tcaaatctaa gattacttga tgctggacat ggatatagtt atggactgga
    41041 tcacgaaaaa ctttttccat aggcagcatt tgatatttat aaggggttgt cataatgtag
    41101 ctcctttatg ttattacgtt gttccattct ctggatttgg ttgttccatt gctccagctg
    41161 gatttctttt tttagccagt cggtagaatc ctgtaaatca cttgcagaat acagcttttc
    41221 aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa aggggttggt caagcagttc
    41281 ttcaagtgtt gccattgtag caggatccac tgcaggatag ccatttgtac caaagtcttc
    41341 ttttaaagca gattgataaa atcgcttcat gacttgcccg cgtttttcct gattaccgtt
    41401 gacacttaag tacatcataa cagaaatccc atttttgatc cgacgctgag cgataccggc
    41461 aaattttttg ccatggatgc ttaaatcata agttccagga caataagaat gggtgatttc
    41521 gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc aataggagca tttgctcgta
    41581 agcgtcatct atggttattt tttttgtgtc actttggggg aaaatcaaag aaatattcaa
    41641 aattccttca tctgatacga tccctaatcc acctgaattt ctgatcactg gagcgtaacc
    41701 tgcatctttc agtgtttgga tcccgtcact tagaaaagga gttcgaacat ccttcatacc
    41761 taagatgact gtgcagtcat attgccagaa atgtaaaaag atttgctggt gttctttaga
    41821 atattccgtt aatatatctg ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg
    41881 ctgatctagt cgaatcgcag ggttcattca accggctcct ttgtataaat tcatattttc
    41941 tacaatatta tactctaaaa tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg
    42001 gacaagttgg ttgacaaaac aaaagaaact aggaacaatg tagctatgag aagtaaagga
    42061 gaatgaataa tggaattatc acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta
    42121 aaacagcaag gggaagtgca agatttttat tttgatttga aagggcaaat ggtgacaatt
    42181 ggcgatacgc tgtacatacg ttataaagag attcaggagg agacgggcga tgagattcct
    42241 gtaacgatca aattagttcc agatggtcat gtacagctga tccgtgcagg agaaatgcgg
    42301 atgcgtttga aatttggcta taaagaacgg ttagaaacat cctatcgaac accatacggt
    42361 atgatccaga ttgctacttt tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca
    42421 gcagggaagg ttcgaatcga ttatgatctg ttcatgggac cggaaaaaat cggcgaatat
    42481 tatttgacct tggattttac agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga
    42541 ttttgtatga taaggagtag actgtgaaag gacgtgtcct gattggaaat tagtgtattt
    42601 gaaggtgcaa acaaaagcga attatcaatg atcgaagtag cacacgcgat cttagaacaa
    42661 cgtggcgatg tcatggattt ttctgattta gctaaccaga tccaaaacta tcttgggaaa
    42721 tcagacagtg aaattcgtga ttcactcgca caattttata ctgacttgaa tattgacggc
    42781 agctttatct cattagggga taatcgttgg ggcttgcgtt catggtatgc tatcgattcg
    42841 atcgatgaag aagtaaacca tggaatggac gaagaggatg aagatactcc acgtcgccgt
    42901 aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag acgcgatcga ttacaatgac
    42961 gatgacccag aagatacaga tctgacagaa gaagatgacg atgatctgtt tgatgacgat
    43021 gatgatgaag atgaagaaat cgctgcttat aattctgact tgcaagaaat cggagcagat
    43081 gacccgacag atgatgaaga agacttgcca ggaaatatcg aagaagattt aagtatcata
    43141 gacgaagacg atgatgatga agattatgaa gaggaagaat tttctgactc tgaaaaagaa
    43201 taaacattat tttcttacga gaggttgtga cagagacgat cagcctatca gaataaggag
    43261 gtgtgacacg atttttgtca caccttcttt tttgtatata aaaatcccaa gtaaatacat
    43321 aaaaaataaa aaaattgaaa cttttagcgc accttgtacg tcatattaag tgaggcagaa
    43381 aggagtggag aatgattttt actggatata agaaaaaacg gcagatcgaa aaataccgtc
    43441 acctgctgaa tggactcatc gaaactgatt atcaaaaaat gttccgtatc acgatgaatt
    43501 acgtacatga taaagaagag gccttagatg tgatgcaaga tagttttcat aaagccctga
    43561 actattttga aaaagaaaaa gagatcgagc attttagtgc atggttttat cggatattga
    43621 tccgcacggc attagatagt tggcgcagac aaaaaaggac gcctgtggac ttatttgaaa
    43681 ttcaagatat cccccaacag ttacctttag aaacttccgt tgcagagctc catatgattt
    43741 tagataaaat agacagtccg gaaaaagaga ttctgatcct ctactttttt gaaggatttc
    43801 gtttgaaaga aattgcgact atcttggata tgaatgaaaa cacagttaaa acgaaaatgt
    43861 accgctcatt gaatgcttta cgtcaaatcc tagaatgaag gagagaagag tgccatgtat
    43921 tcccaaaaag aaaatttaaa aaaactaaag aaaacttata aagaacaagc aattcccaaa
    43981 gggcttaaag aggagatccg ccagcgtttt attatcgaag agcaatcatt tataaaagca
    44041 aagcggagaa agagacgtct ccaagggttc ggagtatctt tggctatcgt aatgattaca
    44101 acaagtagcc ttctttttaa tagtcaagtt cgaagctttg cagaagattt acccatctta
    44161 ggatcagtca ttgaattgat cttaggcgaa cgctttactg atcgatctga aaaaatcgat
    44221 attcaggtac cgaagatcaa tacacaaaat gagaaagaaa ataaaacaat tcatggatta
    44281 aatcaaaaat actttcgcga aggacaagca gattttgaaa aagctgaaaa agaatatgga
    44341 aattttgaaa cggatcatta ccaagttttg ggagattatc agaaaatcgt agacgataac
    44401 cggtttctcg tgattgaaag gcagatcacc caaacagcag cagatagcca tgttgaaaaa
    44461 agatacgaca caatcgacaa gaaaaacagt gtccagctct cacttccact tttattcaaa
    44521 gatgacacct atctatctgt tttgacaaaa gaagtcaaac ggcagatggc cgaacaagtc
    44581 aaggaagatc catctaagta ctattggact gagcaagata ttcaagaagg gacgatagaa
    44641 aaaccaacac ttgttacgcc tacacgaagt ttttatttga acaaagagca tcaattagtt
    44701 ttgacttttt ctcaatacga aattgctcct ggttatatgg gtacaccaga atttgtcatt
    44761 cctaagtccg tgacaaaaac gattttggca tcggaagatt atttggatca ttagacgaga
    44821 agtagttgcg actagcagtt tcaattttca agtaagtaca agtaaataat aagaagagaa
    44881 aaagagaaag aagagatata gtgaaaagaa attatttagt tatcagtatt attgctttat
    44941 ccgtgctttt gtttttcgaa attacttcgg tggcctcctt gatcaccgaa gtaaaaggac
    45001 aaacacactc ttcggagcat tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta
    45061 aaagccaatc aactgaaaca gctaacgtca aagaagccat tcaagaatca gcggaaaagg
    45121 tacaaagtca gtcatcacaa tcaacacaag acagtacaaa aacgagccga gatccggctg
    45181 tcaaaagcgt cgcaatcagc tttgatgatg gaccaggagc gaccacaaca ccgcagcttc
    45241 tgcgaatctt aaaagaaaaa aacgtgcatg ccactttttt cgtcttagga gaaaatacgg
    45301 cgcagcatcc tgaaatcgtg aagcagacag ctgaagcagg gcacgagatt ggcaatcaca
    45361 catacgatca tcagaatctg gccactcttt ctgcccagtc gatgacggag gaagtgacga
    45421 aagcagacac tgagatcaaa aaagtcactg gcaaaacccc cacttttgtt cgtccgccct
    45481 atggttctgt cacgaatgta ggagcaacga tcatccagcg accgatcatc gaatggtctg
    45541 ttgattcaga agattggaag acaagaaatc ctgatttgat tttgcagaag atccaagcaa
    45601 cagtatacga tggggcaatt attttattcc atgatatcta tcctgaaacg attcgtgccg
    45661 ttcctcaagt gatcgattat ctgaaagagc aagggtatcg catcacgaca gttggggatt
    45721 tattagggca tccaacggct gtcgagaact attatggcag aaacgatcat cggccagttc
    45781 agtaagtgag acaagtatct ttaataaata acacatttta ataagtagtt gaacacataa
    45841 ctaccttact tgcaaaggct taacaatatc tatacaggta caacaaaaaa ctgtccacca
    45901 attttctttg gcggacagtt ttcacttaaa aataaagaag caactattcg taaattatgc
    45961 taaaatagca gtagaacgcg tcctgaggga atcgaacccc cgtctcaaga accggaatct
    46021 tacgtgatat ccactacact aaggacgcaa gcactgggat aattgtaact aaacagataa
    46081 aaaattacaa ggggattgga caaaaaaatg aaatttgagc aaaatttttc acaaaaacag
    46141 cagcagacac agaaactagc catgacgcaa cagttgcaac agtccatcca agttttacaa
    46201 tataacagtg aggagctgtt ggcattcgtt gagaatcaag caatggaaaa tccattggtg
    46261 gaagttgttg agccggaatg gcagccagat tacataaaag cttcttcatc ttcttatgaa
    46321 ggagaagaga caaattacct aaatcagatt cctgatacaa aagggtcttt atttgattca
    46381 ttgatcgaac aagtccattt gaattaccga gatacttttt tgaggaaaat cgttctttat
    46441 cttgtagaat acatcgattt gaacggattt ttgacgatta gtttagaaga agccggaaaa
    46501 gagattggag ctacgcctat ccaaatgctc gatgcgttga ctctgattca gcaattagaa
    46561 ccagctggag taggtgcacg tagtttgcaa gaatgtctca tgcttcaaac agaacgagat
    46621 gattatgcgc cggaattagc ttatatcgtg ttggaagaat gttttgagga actggtggag
    46681 cgaaaatgga aagaaatcgc acaacgtttt tctgttgatc tacatgctgt tcagcagata
    46741 ttcgactatt tacaaacgtt gagtccaagc cctggccgga ttttcgaccg tagcagcgaa
    46801 ttgtacatta ttcctgatgt ccgggtatta gtggacgacg ataaaaatgt acaagttatc
    46861 tcaaaccgaa aaaatcagcc aaatatacgc tttcaagaga gttacttcaa acaaatgtca
    46921 caacaagcag ataaagaaac tgaaaattat ttaaaagaac ggaaacagga attcgaatgg
    46981 ttgaagaaaa cgatcctaca acgtggggat acaattttgc gggttgctca agtcattgtt
    47041 tcccggcaaa aagctttctt tatcgacaag gaacgtccaa tcaagcctct tacgttaaag
    47101 gaagttgcaa ctgagataga tgtgcatgaa tccacggtca gtcgtgcagt caacgggaaa
    47161 tatttggaaa cagacttcgg tgtatttgaa ttaaaaaaat ttttcactac acgaattcct
    47221 acgaatagta cagaacagac agaagacctt tcagcagata cagcgaagaa aaagctgcag
    47281 gaattagtcg atcaagaaga caaaaacaag ccgttgtctg accagaaatt ggttgaattg
    47341 ctgaaaaaag atgagatagc gatctcaaga cgaacagtag caaaatatcg tgatttatta
    47401 ggaatcccga gttcatcaaa aagaaaacgt tatgataact aatcagtcaa aagactagaa
    47461 aaagtatttc actttttcta gtctttttag ttgaaaaacg agagcaaaac tgttagactt
    47521 acagtcgtga ggttgagatt tccctttatt gatgtgaaat tcgaatagtt aatttctttt
    47581 tttatcgtat atgggtcgtt ttaagtctac attggacgta aagtgtccaa ctaaggagat
    47641 cgctatgctg gatgaaataa aaatgattga atctgttgca ccagatatta tcgaagtgtt
    47701 gcaagaacgc tataaaattt tacgaaatat ctattggatg cagccgattg gacgtcgaag
    47761 tttatcggaa agtatgggga tcacagaacg agtattgcga acagaaacag atcttttgaa
    47821 aaatctgaat ttgatcgaca cctcaaaaag cggtatgacg ctgacaacta agggcgaaga
    47881 agtttatcaa agccttgaaa acttcatgga tcagctttta ggtacgcatc aaaccgaaca
    47941 acagttggca gaatattttg gtaaccaacg ctgtatcgtt gtttcaggaa acagcgaaga
    48001 gcagaccaaa gtggctgatg cattcggcga agcgctgagc gaagccctag accgactact
    48061 tccagaaggc gagaatatca tcgctgtgat gggaggaact actatggctg tggtagccga
    48121 acaattgtct aatctggaaa ataagaaacg gcataacctg tttgtccctg caagaggcgg
    48181 gattggtgaa gcgataactg tacaggcaaa ttctgtcagt gcaagaatgg ctgctaaagc
    48241 aaacggtaac caccgtgcac tttatgttcc ggaacaatta agtctggcaa cttacaattc
    48301 tttactcaat gagccatcta tccaagaagt cttaaacttg attagtgaag cgaattgtgt
    48361 tattcatagt attgggcgtg cattacacat ggcagcacgt cgtaagatga ctgaaaaaga
    48421 attagtcatg ctgaaacaag caaatgctgt tgcggaatct tttggctact ttttcaatga
    48481 aaaaggtgaa gtcgtttata aggtccctag aatcggcata gaattgcaag atttggaaaa
    48541 agtcccgatc atcatggcaa tcgcgggcgg aaaatcaaaa gctaaagcaa ttcgagctta
    48601 tatgaaaaat gcaccaaaac aaacgtggct catcactgat gaagctgctg caaatgagat
    48661 tttaaaaggg gtaacccttt aaaataaata tttttttgat tttcataagg aggaaatctt
    48721 aaatgacagt taaagtaggt attaatggtt ttggacgtat tggacgtcta gcattccgtc
    48781 gtatccaaga tgtagatgga atcgaagtag tagcaatcaa cgacttgaca gatgctaaaa
    48841 tgttggcaca cttgttaaaa tacgacacaa ctcaaggacg tttcaacgga acagttgaag
    48901 ttcatgaagg ttcattcaac gtaaacggaa aagaagttaa agtattagct aaccgcaacc
    48961 cagaagaatt accatggggc gaactaggcg tagatatcgt tctagaatgt actggtttct
    49021 tcacttctaa atcagcagct gaaaaacatt taacagctgg tgctaaacgc gttgttatct
    49081 ctgctcctgg cggaaacgat gttccaacaa tcgtttataa cacaaaccac gaaacattaa
    49141 ctggtaaaga aacagttatc tcaggtgctt catgtactac taactgttta gctcctatgg
    49201 ctaaaacatt gaacgacaaa tttggtgttg ttgaaggatt aatgacaact atccacgctt
    49261 acacaggtga ccaaatgact ctagacggac ctcatcctaa aggtgacttc cgccgtgcac
    49321 gcgctgctgc tgcaaacatc gttcctaact caacaggtgc tgctaaagct atcggtttag
    49381 taattcctga attgaacggt aaattagacg gagctgctca acgtgttcct gtaccaacag
    49441 gttcattaac agaattagta acagttcttg aaaaagaagt aactgttgac gaaatcaatg
    49501 cagcaatgaa agaagcttca aacgaatctt acggatacaa cacagacgaa atcgtttctt
    49561 cagatatcgt tggtatgact tacggttcat tatttgatgc tacacaaact aaagtaatga
    49621 cagttggcga caaacaatta gttaaaactg ttgcttggta cgacaacgaa atgtcataca
    49681 ctgcacaatt agtacgtact ttagaatact tcgctaactt ataagataga caaacgtaca
    49741 agtgtaaatt atagacatgt acatttaaaa gcggggaagc aatcgcgctt cttccgcttt
    49801 ttttgataat aagtttattc aggaggaaat aaaatggcta agaaaacagt gaaagatatc
    49861 gatttaaaag acaaaaaagt tcttgtccgt gttgacttca acgttccttt gaaagacggc
    49921 gtgatcactg acgacactcg tatcaaagca gcattgccaa caatcaacta cgttctagaa
    49981 caaggcggaa aagcaatcct tttctctcac cttggacgtg taaaaacaga agaagataaa
    50041 gaaggaaaat ctttagctcc agttgcaaaa cgtctaggcg aattattagg taaagaagta
    50101 acattcgtac ctgaaactcg cggagaacaa ttagaagaag caatccgcaa catgaatgac
    50161 ggcgacgttg tcgtattcga aaatacacga tttgaagata ttgacggtaa aaaagaaagc
    50221 aaaaatgata cagaactagg taaatactgg gcttcattag gcgatgtatt tgttaacgat
    50281 gctttcggta cagctcaccg tgcacatgct tctaacgtag gtatcgcttc aactggtatc
    50341 ccaacagttg ctggattctt gatggaaaaa gaaatcaagt tcatcggtga agcagtagaa
    50401 gaaccaaaac gaccaatgat tgcaatcctt ggtggcgcaa aagtttctga taaaatcggc
    50461 gtaatcgaaa acttgattcc taaagcagat aaaatcttga tcggtggcgg aatgacttac
    50521 acattctacg aagcaaaagg aatcaagatc ggtaactctc tagtagaagc tgataaagtg
    50581 gaactagcaa aagaattaat cgaaaaagca ggcgacaaac ttgtcttgcc aatcgacaat
    50641 gtctgtgcac cagaattttc aaatgatgtt gaaacacaag taatcgaagg agacattcct
    50701 gatggtttga tggctttaga catcggccca gcatcagtaa aattatttgc tgatacatta
    50761 caaggtgcaa aaacagttgt ttggaacgga cctatgggtg tatttgaaat gagtaacttt
    50821 gctaaaggta caatcggtgt ttgtgaagca atcgctaact tagaaggcgc aacaacaatc
    50881 atcggtggcg gagactcagc cgcagcagca gaacaattag gatttgctga caaattcaca
    50941 cacatctcaa caggtggcgg cgcaagcttg gaattgcttg aaggtaaaga attaccagga
    51001 ctagcagcaa tcaacgataa ataaacgacc aagtttgctc aattctgacg agtaatcgaa
    51061 caaaaggtga agggagtttt catgactccc tagccatttt tcaataaatt aaggagtgtg
    51121 ttcctcaatg cgtaaaccta ttatcgcagg taactggaaa atgaacaaaa ctgcatcaga
    51181 agcaaaagaa tttgctgaag cagtaaaaaa caaaatccct tcaaatgaca aagttgattc
    51241 agtcatcgga tctcctgcat tattcttaca agaattaatg acatcagctg aaggtacaga
    51301 actaaaaatc tcagcacaaa actgctactg ggaaaactca ggtgcgttca ctggtgaaac
    51361 ttcaccagca gctcttgctg atctaggtgt tcattatgtg atcatcggac attcagaacg
    51421 tcgtgaatat ttccatgaaa cagacgaaga catcaataaa aaagcgaaag ctatctttgc
    51481 aaatggtatg acaccaatcc tatgctgtgg cgaatcttta gaaacctatg aagcaggcaa
    51541 aactgctgaa tggatcgaag gacaaattac agctggttta gctgatcttt cagctgaaca
    51601 ggtgtcaaac atggttattg cttatgaacc aatctgggca atcggtactg gtaaatcagc
    51661 tgacgcacaa atcgctgacg aaatttgtgg agttgtacgt caaacagttg aaaaacttta
    51721 tggaaaagaa gtatcagaag cagttcgtat ccaatacggc ggttctgtaa aaccagaaaa
    51781 tattgctgaa tacatggcaa aagaaaatgt tgacggtgct ttagtcggtg gcgcaagctt
    51841 gcaagcagat tcattcttgt cattgttaga cgctgtaaaa taattgttga aaatttgcga
    51901 aaaatgcaag aaatgtttgc actaatgagc aaaattaact aaaatcgtat atgagggaaa
    51961 tcccttagga aaaaacaaac tcaaaggaga gacaaaacat gtcaattatt actgatgttt
    52021 acgctcgcga gatcttagac tcacgtggta acccaacaat cgaagtagaa gtatacacag
    52081 aaagcggagc ttttggccgc ggaatggttc cttcaggtgc ttcaactggt gaatacgaag
    52141 cagttgaatt acgtgacgga gacaaagctc gttatggcgg taaaggtgta actaaagctg
    52201 tcgacaacgt aaacaacatc attgctgaag caatcattgg ctacgatgtt cgtgaccaaa
    52261 tggctatcga caaagctatg atcgctttag acggaactcc taacaaaggt aaattaggtg
    52321 cgaacgctat tttaggtgtt tctatcgctg ttgcacgtgc agctgctgac tacctagaag
    52381 tacctttata ccactaccta ggcggattca acacaaaagt attgccaaca ccaatgatga
    52441 acatcatcaa cggcggatca catgctgata actcaatcga cttccaagaa ttcatgatca
    52501 tgcctgttgg cgctccaaca ttcaaagaag ctctacgtat gggtgctgaa gtattccacg
    52561 cattagcttc aatcttgaaa gctcgcggat tagcaacttc tgttggtgac gaaggtggat
    52621 ttgctccaaa ccttggttca aacgaagaag gttttgaagt aatcatcgaa gcaatcgaaa
    52681 aagctggcta tgtacctggt aaagacgttg ttcttgctat ggatgctgct tcttcagaat
    52741 tctacgacaa agaaaaaggt gtttacgtac tagctgattc aggcgaaggc gaaaaaacaa
    52801 ctgacgaaat gatcaaattc tacgaagaat tagtttctaa atatccaatc atctcaatcg
    52861 aagacggatt agacgaaaac gactgggacg gattcaagaa attaactgac gtattaggcg
    52921 acaaagttca attagttggt gacgacttgt tcgtaacaaa cactcaaaaa ttatctgaag
    52981 gtatcgaaaa aggaattgct aactcaatcc ttatcaaagt gaaccaaatc ggtacattaa
    53041 cagaaacttt cgaagctatc gaaatggcta aagaagctgg ctacacagca gttgtatctc
    53101 accgttctgg tgaaacagaa gattcaacaa tctctgatat cgctgttgca acaaacgctg
    53161 gccaaatcaa aactggttct ctatcacgta ctgaccgtat tgctaaatac aaccaattac
    53221 tacgtatcga agaccaactt ggtgaagttg cagaatacaa aggtttgaaa tctttctaca
    53281 acttaaaaaa caaataattc ttttctaatg aagatacatg atcgactatt gaaagatagt
    53341 cgagtatgat cacttagtta agtaatcgaa ctctctactt gctgaaatat cagcaggtag
    53401 agagtttttt tgcaactagc ttctgtatat gatgtttaca aagaatggta caagaaaaga
    53461 tcatccttga aaaaggaaag ctaacaataa ctagcatcat ataagagctt ttaaggatag
    53521 aacagtagaa taggaatcta tgagcaagtc ctctcgtata aataaaatag gagggaagta
    53581 gcagcatatt tttcataaag tggctctctt tcttcatttt tactttggtt gctcctattt
    53641 gaaaattcga gcacactcaa atacaataaa aagaaatggc aagggagagg attagatgaa
    53701 aaaagaagaa aaaatcgcga ttttacaaga gatcatccga attaaatcag tgaatggaaa
    53761 cgaaggtgaa gtagcagcct atttgaataa gcttttggca aagcatgaca taacaggaga
    53821 gattgtttct tacagagatg gtagagataa tttgattgct cgttaccaaa agggccaatc
    53881 aggaaaagtg ttaggcctat ctgggcatat ggatgtcgtg gcggctggtg atgaatcgtc
    53941 ttggacctat gcaccctttg cagcagaaat ccatgggaac cgtttgtacg gacgtggtgc
    54001 tacagatatg aagagtggct tggcagcaat ggtgattgcc atgatcgaac tgaaagagtc
    54061 aggaaaacca tttaacggaa cagtcaaatt attagccact gtcggagaag aagtgggtga
    54121 actaggtggg gaacagctca cgaaagcagg ctatgtagat gacttagatg ccttgatcat
    54181 cggagagcca accaattaca gtttgatgta tacgcacatg ggatcaatca actatacagt
    54241 cacctcgcac ggaaaagaag cgcatagctc tatgccagac caaggttata atgcaatcaa
    54301 tcacctaaat gaattcatta cgaaagcaaa tgctgaaatg aatcacctcg ctgaaacaat
    54361 cgagaatcct gtcctaggaa aaacaatcca taatgttaca ttgatcagtg gcgggaatca
    54421 agtcaacagc atcccaagcc atgcgcagct ccaagggaac attcgatcga ttccagaata
    54481 cccaaatgat aaaatcattg cattgctgca atcaatcgtc aatgaattga atcaagagac
    54541 agactatcat ttggaattga tgatcgatta caacaaaata cctgtcaaag ctgatccaga
    54601 ttctcctttg attcattgca ttcagcaaca attcagccag ccattacctt tagttggagc
    54661 ggcagcaaca acggatgcag ccgaatttac gaaagctaac cattccttcg actttgtcgt
    54721 ctttgggcct ggtgtcgtta ctttgcctca tcaagtagat gagtatgttg aaatcgataa
    54781 ttatttggat atgatcgaaa aatatcaagg aattatttta tcctacttag cgtaagattt
    54841 ttttaataaa tgaaaaaaca gagccagatt agcatta
//
LOCUS       DAYYAR010000005        49509 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000005 DAYYAR010000000
VERSION     DAYYAR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.005"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            558..3197
                     /locus_tag="ACHAT0_000237"
     CDS             558..3197
                     /locus_tag="ACHAT0_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8450214.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="ACHAT0_000238"
     CDS             complement(3365..4063)
                     /locus_tag="ACHAT0_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8450215.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            4281..5426
                     /gene="tgt"
                     /locus_tag="ACHAT0_000239"
     CDS             4281..5426
                     /gene="tgt"
                     /locus_tag="ACHAT0_000239"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8450216.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            5523..5903
                     /gene="yajC"
                     /locus_tag="ACHAT0_000240"
     CDS             5523..5903
                     /gene="yajC"
                     /locus_tag="ACHAT0_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8450217.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            6208..8805
                     /gene="adhE"
                     /locus_tag="ACHAT0_000241"
                     /gene_synonym="adhC"
     CDS             6208..8805
                     /gene="adhE"
                     /locus_tag="ACHAT0_000241"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8450218.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            9034..10140
                     /locus_tag="ACHAT0_000242"
     CDS             9034..10140
                     /locus_tag="ACHAT0_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8450219.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            10157..11185
                     /locus_tag="ACHAT0_000243"
     CDS             10157..11185
                     /locus_tag="ACHAT0_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8450220.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            11191..11670
                     /locus_tag="ACHAT0_000244"
     CDS             11191..11670
                     /locus_tag="ACHAT0_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8450221.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            11686..12981
                     /locus_tag="ACHAT0_000245"
     CDS             11686..12981
                     /locus_tag="ACHAT0_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8450222.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            12992..13816
                     /locus_tag="ACHAT0_000246"
     CDS             12992..13816
                     /locus_tag="ACHAT0_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8450223.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            13981..14880
                     /locus_tag="ACHAT0_000247"
     CDS             13981..14880
                     /locus_tag="ACHAT0_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8450224.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(15006..15875)
                     /locus_tag="ACHAT0_000248"
     CDS             complement(15006..15875)
                     /locus_tag="ACHAT0_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8450225.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            16028..17488
                     /gene="rhaB"
                     /locus_tag="ACHAT0_000249"
     CDS             16028..17488
                     /gene="rhaB"
                     /locus_tag="ACHAT0_000249"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8450226.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            17485..18741
                     /gene="rhaA"
                     /locus_tag="ACHAT0_000250"
     CDS             17485..18741
                     /gene="rhaA"
                     /locus_tag="ACHAT0_000250"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8450227.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            18755..19600
                     /gene="rhaD"
                     /locus_tag="ACHAT0_000251"
     CDS             18755..19600
                     /gene="rhaD"
                     /locus_tag="ACHAT0_000251"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8450228.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            19602..19913
                     /gene="rhaM"
                     /locus_tag="ACHAT0_000252"
     CDS             19602..19913
                     /gene="rhaM"
                     /locus_tag="ACHAT0_000252"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8450229.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            19995..21146
                     /gene="fucO"
                     /locus_tag="ACHAT0_000253"
     CDS             19995..21146
                     /gene="fucO"
                     /locus_tag="ACHAT0_000253"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8450230.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            21273..22031
                     /locus_tag="ACHAT0_000254"
     CDS             21273..22031
                     /locus_tag="ACHAT0_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8450231.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            22131..22775
                     /locus_tag="ACHAT0_000255"
     CDS             22131..22775
                     /locus_tag="ACHAT0_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8450232.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            22782..23789
                     /locus_tag="ACHAT0_000256"
     CDS             22782..23789
                     /locus_tag="ACHAT0_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8450233.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            23805..24647
                     /gene="kduI"
                     /locus_tag="ACHAT0_000257"
     CDS             23805..24647
                     /gene="kduI"
                     /locus_tag="ACHAT0_000257"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8450234.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            24667..25470
                     /locus_tag="ACHAT0_000258"
     CDS             24667..25470
                     /locus_tag="ACHAT0_000258"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8450235.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25719..26783)
                     /gene="fni"
                     /locus_tag="ACHAT0_000259"
     CDS             complement(25719..26783)
                     /gene="fni"
                     /locus_tag="ACHAT0_000259"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8450236.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26780..27865)
                     /locus_tag="ACHAT0_000260"
     CDS             complement(26780..27865)
                     /locus_tag="ACHAT0_000260"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8450237.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="ACHAT0_000261"
     CDS             complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="ACHAT0_000261"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8450238.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="ACHAT0_000262"
     CDS             complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="ACHAT0_000262"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8450239.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            30108..30767
                     /locus_tag="ACHAT0_000263"
     CDS             30108..30767
                     /locus_tag="ACHAT0_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8450240.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            30852..31757
                     /locus_tag="ACHAT0_000264"
     CDS             30852..31757
                     /locus_tag="ACHAT0_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8450241.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(31759..32391)
                     /locus_tag="ACHAT0_000265"
     CDS             complement(31759..32391)
                     /locus_tag="ACHAT0_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8450242.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    32542..32667
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            32711..33235
                     /gene="infC"
                     /locus_tag="ACHAT0_000266"
     CDS             32711..33235
                     /gene="infC"
                     /locus_tag="ACHAT0_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8450243.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            33307..33508
                     /gene="rpmI"
                     /locus_tag="ACHAT0_000267"
                     /pseudo
     CDS             33307..33508
                     /gene="rpmI"
                     /locus_tag="ACHAT0_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355798.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
     gene            33561..33920
                     /gene="rplT"
                     /locus_tag="ACHAT0_000268"
     CDS             33561..33920
                     /gene="rplT"
                     /locus_tag="ACHAT0_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8450244.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            34172..35509
                     /locus_tag="ACHAT0_000269"
     CDS             34172..35509
                     /locus_tag="ACHAT0_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8450245.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            35557..37686
                     /locus_tag="ACHAT0_000270"
     CDS             35557..37686
                     /locus_tag="ACHAT0_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8450246.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            37661..38350
                     /locus_tag="ACHAT0_000271"
     CDS             37661..38350
                     /locus_tag="ACHAT0_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450247.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(38758..39371)
                     /locus_tag="ACHAT0_000272"
                     /pseudo
     CDS             complement(38758..39371)
                     /locus_tag="ACHAT0_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(39507..39701)
                     /locus_tag="ACHAT0_000273"
     CDS             complement(39507..39701)
                     /locus_tag="ACHAT0_000273"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450248.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(39751..40191)
                     /locus_tag="ACHAT0_000274"
     CDS             complement(39751..40191)
                     /locus_tag="ACHAT0_000274"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8450249.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(40195..41040)
                     /locus_tag="ACHAT0_000275"
     CDS             complement(40195..41040)
                     /locus_tag="ACHAT0_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8450250.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            41189..42607
                     /gene="pepV"
                     /locus_tag="ACHAT0_000276"
     CDS             41189..42607
                     /gene="pepV"
                     /locus_tag="ACHAT0_000276"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8450251.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(42664..43611)
                     /locus_tag="ACHAT0_000277"
     CDS             complement(42664..43611)
                     /locus_tag="ACHAT0_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8450252.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(43834..44184)
                     /locus_tag="ACHAT0_000278"
     CDS             complement(43834..44184)
                     /locus_tag="ACHAT0_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8450253.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            44384..45463
                     /gene="pepA"
                     /locus_tag="ACHAT0_000279"
     CDS             44384..45463
                     /gene="pepA"
                     /locus_tag="ACHAT0_000279"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8450254.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            45607..45927
                     /locus_tag="ACHAT0_000280"
     CDS             45607..45927
                     /locus_tag="ACHAT0_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8450255.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            45949..46413
                     /locus_tag="ACHAT0_000281"
     CDS             45949..46413
                     /locus_tag="ACHAT0_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8450256.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            46618..47223
                     /gene="ytpR"
                     /locus_tag="ACHAT0_000282"
     CDS             46618..47223
                     /gene="ytpR"
                     /locus_tag="ACHAT0_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8450257.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(47262..48551)
                     /locus_tag="ACHAT0_000283"
     CDS             complement(47262..48551)
                     /locus_tag="ACHAT0_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8450258.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            48979..49458
                     /gene="rlmH"
                     /locus_tag="ACHAT0_000284"
     CDS             48979..49458
                     /gene="rlmH"
                     /locus_tag="ACHAT0_000284"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8450259.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT        15667 a         9045 c        10631 g        14166 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttgtccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca
     1201 ctgccattgg catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg
     2521 tcagtcaagg acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ctttaataac accaatatac catgtatcaa aaaatatttc tgtcataatg tttttctttt
     4141 ttttgttatt cataaaaaaa ggataaaaaa aagaatatta aataaagtag aaggaagccg
     4201 agggaacttg caaaaagcac ttgaaacgat tatgatggtg ggagttgtca agatatttta
     4261 tatagaaagt agggaaatga atgacgcaac ctgccattcg ctatcgttta atcaagaaag
     4321 aaaaacatac tggcgcgcgt ttaggggaat tgatcacgcc acacggaact ttccccacac
     4381 caatgttcat gccagtagga acattagcta cagtaaaaac aatgtcccca gaagagttga
     4441 aagaaatggg tgctggcgtt attttaagta atacatatca tttatggcta cgccctggtg
     4501 aagacttagt agaagaagct ggcggactgc ataaatttat gaactgggat caaccgattt
     4561 taacagattc aggcggcttt caagtttttt cattaagtga tatgcggaat atcgaagaag
     4621 aaggcgtgca tttcaaaaat cacttgaacg gttcgcgtat gtttttatca ccagaaaaag
     4681 caatcaacat ccaaaataaa ttgggctctg atatcatgat gagttttgac gaatgtccac
     4741 cattcgatga aagttacgac tacgtgaaaa aatcagtaga acgtacgtct cgatgggcag
     4801 aacgaggatt gaaagctcat gctaatccag accgtcaagg attatttgga atcatccaag
     4861 gagcagggtt tgaagatctt cgtcgtcaaa gcgcgaaaga tctagtcggg atggatttcc
     4921 ctggttactc aatcggcggg ttatctgtcg gggaacctaa atcagaaatg aatcgtgtat
     4981 tagaattcac tacaccgttg atccccgaaa acaaaccacg ttacttgatg ggtgtcggtg
     5041 ctgctgattc attgatcgat ggtgttatcc gtggagtaga catgtttgac tgtgtcttgc
     5101 ctactcgaat cgctcgaaat ggcacatgta tgacttctca aggccgtcta gtagtgaaaa
     5161 atgcgaaata tgcccatgat ttccgtccaa ttgatgagaa atgtgattgt tatacatgta
     5221 aaaactatac acgtgcctat atccgccatt tgatcaaatg tgatgaaaca tttgggattc
     5281 gtttgacttc ttaccacaat ttatacttct tgttgaatct aatgaaacaa gtacgtcaag
     5341 caatcatgga tgataatttg cttgaattta gagaagcctt ttttgaagaa tacgggttca
     5401 ataaagaaaa cgcaaaaagc ttctaaagga aaatctttta aaaattaagt aaaaaactag
     5461 tgttatcagc atttttctgc taaagtgttt tttgtagata cagaattagt gaggtgaaaa
     5521 aaatgggaac attaccaatg cttgtgatgt ttatcgcgct attagcaatg tggtttttta
     5581 tgtcacgttc gcaaaagaaa caacagcaag aacgtcaaaa cttgttagat agcatgaaac
     5641 caggtgattc agtcgtaacg atcggcggat tacatggagt tgtttcagaa atcgacaatg
     5701 acaaacggac agttgtttta gactgcgaag ggatcttcct tgagtacgat cgtgcagcaa
     5761 tcaaaacggt caaaccaggg actgtcgtaa caaatgatgc aacgactact gttgaaacaa
     5821 cagaagaacc agctgagaca gttgaagtaa aaactgaaac aacagaaacg ctggaagaga
     5881 cagaagagaa ggaagaaaaa taagtatcaa agcttattta aagacgaggt agggcagaaa
     5941 gatgtcctac ctcatttttt attcaaaaaa gaaagagata agagcatggg atatattgct
     6001 caatattgaa taatcgggtg tgtcatataa ataataataa ataactttat atatttttct
     6061 atacaaacac ctgtaaataa agcaatttta tatttaaaaa taactgtgaa aaaaatcaca
     6121 aaaaagtgtt tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa
     6181 taaccatgac ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag
     6241 acaattgacg tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa
     6301 gaaatggaaa gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca
     6361 gctttggatc aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt
     6421 tacgaagata aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa
     6481 catgataaaa cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct
     6541 gaaccagtcg gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt
     6601 ttcaaatcaa tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt
     6661 gcccaaaaat cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga
     6721 gctcctgaaa attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta
     6781 atgaaccatc caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct
     6841 gcttactcta caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt
     6901 gaaaaaacag caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat
     6961 aatggaatga tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca
     7021 gtaaaagcag aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa
     7081 ttagaagatg cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat
     7141 tctgcgatgg aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg
     7201 attgctgaat tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca
     7261 gttcttgcca tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg
     7321 ttgaatcttg gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat
     7381 gtcaaattcg gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa
     7441 ggcggcatcg gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct
     7501 tatggtaaga actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact
     7561 gtagcgaaac ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa
     7621 aagaactctc tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt
     7681 gaccctggta tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt
     7741 aaaaatgatg tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc
     7801 gtatacgcag gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga
     7861 ggcggttctg caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca
     7921 tctttctttg gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac
     7981 aaaccagaaa aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta
     8041 acgccatttg cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat
     8101 gcattgacgc cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct
     8161 gtcacagcag ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc
     8221 atggcttcag attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat
     8281 ttagaaaaat cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct
     8341 acaatggctg gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat
     8401 aaaatcggcg gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat
     8461 attatccgct ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat
     8521 ttccgtgcag atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca
     8581 acagaggaat tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc
     8641 atcaacatga gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac
     8701 cgtatggctg aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg
     8761 atctctgaat tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta
     8821 gaaaaaagat aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt
     8881 tttttaattt ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag
     8941 acagtttttg ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata
     9001 taaaatgaaa gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta
     9061 cctaaagaat tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa
     9121 gacgataatg gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg
     9181 acagtgtgga attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg
     9241 acaaaaaatc ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa
     9301 ggcgctccgc ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat
     9361 gaagacacga aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg
     9421 aatgacatgc ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat
     9481 aagaatcagt tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg
     9541 aaactgttga atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc
     9601 aaatacttga tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat
     9661 cataactatg ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca
     9721 gagatcattg aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa
     9781 acacttgaag cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat
     9841 acgttgttgg atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac
     9901 ggtattttga aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat
     9961 cgtgctatcc aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca
    10021 atcgggacag gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg
    10081 ccttatggac agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa
    10141 aagaaaggaa acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct
    10201 aggtctgcta ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc
    10261 agatgtaaat gagatagtta ccttgcgctt tgcttatgca agcaacagcc agccagtaat
    10321 tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat
    10381 ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc
    10441 aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta
    10501 ttcgattttt tcagttcctt atctattcaa tgatgaagaa catttcttta aggtgatgga
    10561 taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat ttaccggatt
    10621 gacctattat gattctggtc agcgcagctt ttatatggta gacggtccga tccatacgcc
    10681 ggaagatttg gagggaaaga agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt
    10741 agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca cgtctcttca
    10801 atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg
    10861 tggtgtggct aaatactatt cctatgatga gcatacacgt gtgccagata tcatcatcat
    10921 gaatgatgcc atcaaagaac agctaactga tgagcaggaa aaagccattg aagaagcagc
    10981 gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc
    11041 tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac cgtttcgaga
    11101 ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag acctttatca
    11161 aaagatccaa gagcaaggag cgtagctgtg atgaaaatga ttttaaacaa ggtattagag
    11221 atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca agtattttcc
    11281 agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt cggtcttgtc
    11341 tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacattt agccgtaacg
    11401 gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt
    11461 ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc tgtcacattg
    11521 acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta tctgtcagta
    11581 cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga tacattaagc
    11641 aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc aatagaagcc
    11701 ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc gattgctata
    11761 agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga tgtttctgct
    11821 tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat cgctattccg
    11881 tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa actagtggat
    11941 tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg
    12001 aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc gattggcgga
    12061 gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac agcggtcaat
    12121 atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat catgtattca
    12181 cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct cgtaggcgct
    12241 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
    12301 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
    12361 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
    12421 gcttcagcga tagctgtcat ttactccctg ttgattgcta tgttttacta taaaacggta
    12481 aaactaaaag atatacctaa tatgttgttt caatcagtag caatgtcagg gacgatcatg
    12541 ttcttattgg ctacttcttc catgatgtct ttcgccatgg cgtttaccgg tattcctcag
    12601 gcaattagtt cgttgattat gggcgtaacg gataataaat ttattattct gttattagtc
    12661 aatcttgtgt tattactggt gggtatgttt atggatgtgg cgcctgctat cttgattttt
    12721 acaccaatat ttttaccaat cgtcacaagt gtaggggttg atcctgtaca ttacgggttg
    12781 ttttcaatca tgaatctgtg tgtaggctcc atcacgccgc cagtaggtac aggactatat
    12841 gtcggtgcaa gtgtaggagg cgtaaaagca gaggcaatgc tgaaaccgct tcttcctttt
    12901 tatggggtta tcttagtcgt cttgtttttg attacttact tcccgcaaat cgtcatgtgg
    12961 ctacccaata tgctgagtta ggagaatagt tatgttgaac attacaatta gaggacatga
    13021 tctttccgaa gtccaaacga tagaagatct tgcagaaaaa acaaaagaac aagggattca
    13081 tacactgcag ctcgccttag gtctttcgtt tcctgaaaca ccttctggcg caaatgagat
    13141 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
    13201 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
    13261 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
    13321 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
    13381 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
    13441 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
    13501 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
    13561 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
    13621 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
    13681 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
    13741 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
    13801 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
    13861 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
    13921 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
    13981 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
    14041 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
    14101 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
    14161 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
    14221 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
    14281 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
    14341 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
    14401 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
    14461 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
    14521 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
    14581 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
    14641 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
    14701 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
    14761 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
    14821 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
    14881 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc
    14941 ggttatttta tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat
    15001 atgaatcagc tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg
    15061 cggctaaagt aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat
    15121 tctgtatacc gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt
    15181 ggtggagaac cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat
    15241 tgttgttcta attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc
    15301 agtttgtaaa cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa
    15361 atcagttcat tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc
    15421 gtgccttgtt gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga
    15481 tcccctgttg aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg
    15541 acgttctgac cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga
    15601 taacgctgat attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga
    15661 ttatcattat atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt
    15721 aaatgtgctc gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata
    15781 taaaaaaata aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc
    15841 gaccatgagt ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat
    15901 aatgcaatta tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca
    15961 ttgttaataa atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg
    16021 tggagaaatg aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat
    16081 gaaaagtacg agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa
    16141 cggctttcgg aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat
    16201 tttgattgga tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga
    16261 tacatgggca gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat
    16321 tgcttatcgg gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct
    16381 agataagttg tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt
    16441 gtttgctgaa gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta
    16501 tttagggtat gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca
    16561 gctgctgaat gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc
    16621 gaaggaatta tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga
    16681 acaattttca aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac
    16741 ggcgtcagct gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg
    16801 gacatggtcg cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc
    16861 tgaaaattat acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat
    16921 ggggatgtgg ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga
    16981 attggcagag cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc
    17041 tcgatttttg aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga
    17101 gcagccagtc cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct
    17161 ttgttacgca caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct
    17221 gcacatcgta gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc
    17281 cggaatcatt gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca
    17341 aatgctcagt acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc
    17401 gtttccatgt gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt
    17461 gaataaaatc aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa
    17521 gaaaaatatg caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc
    17581 aaaatttcta tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc
    17641 gaattgaccg gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa
    17701 gagcttagac aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac
    17761 cttcatgcta tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa
    17821 cattttgaaa aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg
    17881 actttctttt cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa
    17941 gttcgtgaat actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg
    18001 aaagaaactg ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat
    18061 ccgattgatc gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca
    18121 gaagagttag atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc
    18181 gctgaagctt atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac
    18241 aaacttgttt gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta
    18301 tcttcattag ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg
    18361 gattcagacc atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc
    18421 cgtaacgaat tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac
    18481 cgtatcgctg cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg
    18541 ctggaaccag ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg
    18601 gcttatacgg aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac
    18661 aaaaatcaag ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac
    18721 gtgttggaat taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc
    18781 gccttatgtg aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga
    18841 aagaaatggc gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga
    18901 tcctgaaaaa gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg
    18961 ctattttatt gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga
    19021 gaatcttgga atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct
    19081 ggaaaacgaa tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg
    19141 tttagaagta gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc
    19201 gatgagtttt acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg
    19261 tacggaatgc ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg
    19321 aacaaatgag attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg
    19381 gccacaacat ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga
    19441 gacagcagaa aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca
    19501 aacaatcacg gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc
    19561 aggttatttg gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt
    19621 gcatgaaagt ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc
    19681 cagaaatgcg tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc
    19741 cgagtacaag tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa
    19801 tggcaaatac atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa
    19861 atccagatgc ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag
    19921 caactgggca accggttgct tggggttctt tcgtttttat tgtgaaaaaa aatacaaatt
    19981 aggaggaata gaaaatgagt aatcgaatga ttttaaatga aacatcgtat tttggaaaag
    20041 gagcgattga gtcgatcgtc cctgaatttc agcagcgagg attcggcaaa gcagctgtca
    20101 ttactgacaa aggattgatc gagcacggta tagcaacaaa agtaacagaa cttctagata
    20161 aagcagcaat cgcttatgct ttatacgacg ggatcgtacc taatccaacg atccaaaatg
    20221 taaaagatgg agtggctttt gttaaagagg cagaggcaga ctgtttgatc gcaatcggtg
    20281 gaggttcgcc aatcgatacg gcaaaagcca ttgggattat tttgacgaat ccagagtttt
    20341 cagacgtcat tagtttagaa ggtgttgctg atacgaaaaa tccatgttta cctatattag
    20401 ctataccaac gacttctgga acagcagcag aagtaacgat caactacgtc atcacagatg
    20461 aagtaaatca ccgaaaattt gtttgtgttg atcctcatga tatcccaatc gtcgcgttca
    20521 tcgacagcga tatgatgatg ggaatgccga aaaaattggc tgcatcaaca ggtatggatg
    20581 ctatgacgca tgctattgaa gggtttatca caaaaggcgc ttgggaaatg actgatatgc
    20641 tgcatttaaa agcaatcgaa atcatcggcc attctttgga agtttctgta gatggcgatc
    20701 aaaatgggcg agaaaaaatg gcattaggac agtatattgc gggaatgggc ttctcaaatg
    20761 tcggtttagg actggtacac gggatggctc acccgttaag tgcatggtac aacattcctc
    20821 atggcgtagc gtgtgcagca cttttgccaa ctgtcatgaa atataacaaa gaatatactg
    20881 gagaaaagta ccgtgagatc gctcttgttc tagggattaa aggagcagct gaaatgtctt
    20941 tagaagatgt aagagaagcg gcttgtggtg agatcgatcg gttgagcaaa gcggttggaa
    21001 tcccagaaac catttctgaa ttaggtgtaa aagaagcaga tattccagca attgcagaag
    21061 atgctttgcg agatgtatgc acaccaggaa atccaagaga aacgacagta gaagaaatca
    21121 ttgctttata tcaaagtttg atgtaaaaac gaagcggatc ttcaacatta aattgcctgt
    21181 aaagagaaat ggtagtttat atctggatat ctcttttaaa ttgatggtat ttccgctata
    21241 atttttctta taaagaaata ggagaatatt atatggaaaa taaaaagcct tatggaactg
    21301 tattgatcaa agcctctaaa atactagact atttagcgga aaaccaagat gtctctttgc
    21361 agacgatcgc aaagggtgtg ggaatgacct cttctacagt cttgaaaata ctagatactt
    21421 tgttgatgat cggctacgta aataaaaatt cagagaaaaa ctatcgttta ggtgcaaagc
    21481 ttattcgcta cgcgaataaa aacattgaac aaatcgattt ggtagagttg actttaccgt
    21541 ttttagaaca gctgcagcaa aagatcgatg agactatcca cttaggggta ttggacaaca
    21601 atgagatctt gtacgtgaac aaactagatc caaaacatca aacgatccgc atgtcttcaa
    21661 aaatcggaat cacccgacca ttatacaatt ctgcgatggg caaagccgta ttagcagaat
    21721 tcagtgaaga gcagtacact gattatcttg aaaagcatcc gttgatccct tacacagaat
    21781 atacgatcac caatgcatta cggttgaaaa aggaaatcca aacggtaaaa gaaacccaca
    21841 tcgcatttga cgatgaagaa atcgaacgtg atattttttg tataggagcc tcaatcgtga
    21901 aaaacgaaca aatcattggt gcatttagtg taagtatgcc gaaatatcgt ttaacagatg
    21961 agacaaaaga acaaataatc agtgccctca agcaaacaaa aagcgaaatc gagcaaagaa
    22021 ttcaaaaata atgaaaaaag cgtttgcaat ttttcttttc tgccgtataa tatgggtata
    22081 gatttccaag ttataaaata aaatttctaa ataggaaata ggtggagaag atgaaaaaaa
    22141 ttgatatttt atctcgattg aaaaacgccg gagtgattgc ggtggtacgc ggaaaaagca
    22201 aagaagaagc tttgaatgct tgccacgcaa tcatcaaagg tggtctaact ggtattgagt
    22261 tgacttttac agtccctcaa gcagatcaag taatcaaaga acttttgtca ttctacaaag
    22321 accaaccaga aatagtgatt ggcgcaggca cagtattaga cgctgtcaca gctcgcttgg
    22381 ctatcttagc tggcgcagag tatattgtta gcccttcctt tgatcaagaa acggcagaga
    22441 tgtgcaatct gtatcaaatt ccttacttac caggatgtat gactattacc gagatcaaaa
    22501 cagcgctgaa aagcggggta gatatcgtca aactatttcc tggaagcgct tatggaccaa
    22561 gtatcatttc tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca actggcggtg
    22621 tgagtctgga caatatgaaa gagtggttcg atgctggtgt cgtaacagtc ggagtaggtg
    22681 ggaacctttt agcacccgca gctacaggag attttgacaa agtaacggaa gtcgcgcaac
    22741 agtatgcagc aaaaatgaaa gaaatcaaga ggtagctgct tatgggaaaa gtagtgacac
    22801 tcggagaaat catgttgcga ttgtccacag agcagggaac ccgtatcgat tcaagtgaac
    22861 gattcgctgc ccattatggc ggaggggaag caaacgtagc aatttcttta gctaattacg
    22921 gacatgaagc gatttttgct acaaaagtac ctgataacgc attgggaaaa gcagtcaaaa
    22981 agcacttgca atgttatgga gtagatacga aacaggtatt atctggtgga tcaagactag
    23041 gtacttatta tatggaaact ggtagtggcg aacgcgccgc ctcagtgatc tatgatcgtg
    23101 ccggatcaag ttttgctgaa atggatcatt tggaatggtc gctagaggag ttattccaag
    23161 gaatagatat atttcatgtt tcaggaatca ccccagcttt gtctaaaaaa tggcaaaaaa
    23221 tgacgaaaca ggtgatagaa gctgcaaaaa aagctggctg cctgatcagc ttcgatatca
    23281 actatcgtgg aaaattatgg acacagcaag aagccagcga agtaatccat cagcttttac
    23341 ctttagtaga tatctgctca gctggaaaaa tggatgctct ttatttgtta ggtatcgaga
    23401 aagcgccaga agcagaagaa caacctctaa tttattatta tcaaaaaatg caggaaaagt
    23461 tcccgaatat tcaggtgttt tattcgacca agcgtaccgt tcattcagca agtgaaaatg
    23521 aattgaaggg gacattgtgg atgaaccatg aatatgtgga atcccaagtc catagaatca
    23581 ccccaatcgt ggatcgagta ggtggcggag atgcttttgc aggtggcgtg ctccatggga
    23641 ttttatccaa tatgtctccg caagaaatca ttgattttgc tacggcagct tctgccttga
    23701 agcatacgat ccatggggac tgcaaccaat ttagtcaaga agaagtcgca caatttttag
    23761 catgcggctc tggaaaaatc attcgataga aggagtagaa gaaaatgcaa gaaatggaaa
    23821 cacgttatac acacagtcca gaagatatcc gtcattattc aacggaacaa ttgagaaaag
    23881 aatttttagt agaaaaggta tttgaaccag ggaaaatcag tttgacttat acgcacaacg
    23941 atcgaatgat ctttggtgga gtgacaccta ctgaaaaaga attagaaatc acattagaca
    24001 aagaactagg tgttgattac ttcttagagc gtcgtgaact aggtgtgatc aatatcggtg
    24061 gaccaggatt catcgagatc gatgggcaaa aagaagaaat gaaaaaacaa gacggatact
    24121 atatcggaaa agaaacacga catgttgtat tttcttctca agatgctgaa aacccagcta
    24181 aattctatat tagttctgtt ccagctcatc ataaatatcc aaatgtaaaa atcagtatcg
    24241 accaagtaaa accaatggag actggtgaag gcctgacact gaatgaacgt aaaatctacc
    24301 aatacatcca tccaaatgta tgcgaaagct gtcaattaca aatgggttat acgattttag
    24361 aaccaggaag cgcttggaat acgatgcctt gtcatacaca tgaacgtcgt atggaagctt
    24421 atgtctactt tgattatgcc aatgaagata cacgtgtgtt ccacatgatg ggcaaaccag
    24481 atgaaacaaa acatttagtt gtagacaatg aacaagccat tatttcgcca agctggtcga
    24541 tccattcagg tgtaggtaca agcaattact cattcatctg ggcaatgtgc ggagaaaata
    24601 tcacctatac agacatggat atggtgcaaa tggatcaatt aaaataagca taagggagag
    24661 aaaatgatgg aatttagtat ggatatgttt cgtttagatg gaaaagtagc attagtcacc
    24721 ggtgctgtct atggtattgg atttgagatt gcacgctctt tagctacagc aggagcaaaa
    24781 atcgtattta ataacttgaa tcaagaatca gttgatgaag gtatagcgca ttataaagaa
    24841 gcaggcatcg atgcaaaagg ttatgtctgc gatgtgaccg atgaagaagc ggttcaagcg
    24901 atggttcaac agatcaaaga agatgtaggt tcagttgata ttctagtcaa caatgcaggg
    24961 atcatcaaac ggacaccgat gatcgaaatg gatgcggcag acttccgcca agtaatcgac
    25021 gtggatttaa atgccccatt catcgtttca aaagcagtta ttccggatat gatcgaaaaa
    25081 ggcggcggta aaatcatcaa tatctgctcc atgatgagtg aattaggacg cgaaacggtc
    25141 agtgcttatg ctgcggcaaa aggcggatta aaaatgctga cgaaaaacat cgcttctgaa
    25201 tacggtcaat acaatatcca atgtaatgga attggaccag gttatattgc tacaccgcaa
    25261 actgctccat tacgcgaaac acaagaaaac ggtgaacgtc atccattcga tcaatttatc
    25321 gttggacgta cgccagcagc acgctggggc gagccaatcg atttggctgg accatctgtc
    25381 ttcttggctt ctagcgcatc tgatttcgtt aacggccatg tcttgtatgt ggacggaggc
    25441 atacttgcct atatcggaaa acaaccttaa aagagcttta gtacactgtt tagtggagag
    25501 gttgtgaaat cagcggattg aacgaatacc gaaggacagc tgattaaaca tcatttattc
    25561 gaaaaaagag gctgggacag aagcatttaa ctccaagaaa taagaaggaa ttcacgaaaa
    25621 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ttacttccgc
    25681 cgtttgtacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
    25741 tctgtgcgtc ttccatactt cactaagtcg atccctcttg cttcgcacca atccttcact
    25801 ggtccggaaa agatcagaga ttgttggtgt agggccgcgg tattcgttgc gccgaccata
    25861 gtataaagca gacgaagctc ttcttgccat tgcttcatca gtataatagt ttcttctacg
    25921 ccgtgattca tcagatgcgt cagtacggtt cctgaagtac ccacggcatt tgcgccgaga
    25981 caaagggctt taaatatgtc ataggcattg cggatacctc cggaggctag gatttccata
    26041 gaagtgtctg cttcatttgc ttcaagcaat gaagagactg tcgattgccc ccagtcagct
    26101 aaataactca gttcccgctt ggagcgacgg gcgttttcga tctgggtgaa gcttgttccg
    26161 cttcgaccgc tgatatcaac tgtatgaact cctaaagcag ctagatcatt gagcgtttct
    26221 cttgtcatgc caaaaccgac ttcttttacg atgaggggga cgtcaatggc tgtttgagtt
    26281 tcttgaataa gtactttcca attggtaaaa tcacggtctc cctcaggcat aaccaactct
    26341 tgaggtgcat tcaaatgaat ctgtaaggca tctgcgtgaa acaatcggat agcttctttg
    26401 gctctttcga cagaagtccc tgcaccgatg ttggcaatga ttttcccgtg aggatattct
    26461 tgtcgcataa tcgtataagt atcggctaaa gaagggactt tcaaggcagc gctgactgaa
    26521 ccagtggcaa tcatcaaatc tgcttctctt gcgacgattg caagatcttg gttgattttt
    26581 tttgtttttt cgcttccgcc agtcatcgca ttgatataaa atgggctgct tagtgtaaac
    26641 ccaactgcct gtgtacttag atcgacatca gaaactgcta tttgcggcaa agggttatgg
    26701 atgatccgta cgaagtcaaa ttcgtttttt tgtttatcat gaaaggcttt agctagtgaa
    26761 acatgttcgt cttttcgatt catcggtttt cctttctttg atcgctatag acatgtaacg
    26821 gcagtggagt gatttctgct ttttcccatg cactcattaa aggaagaatg ccagatttct
    26881 ggtcaacaat tacgattccg caatcgcccc cacctgcacc agaagatttt gcggctcctt
    26941 catactgttc agctaaatta cacaatttgt tcaaagcagg cgtttcgatg acgaccccag
    27001 tgattgcaga taaatcatgc agtaattgtc ggttttttcg aatcatctgt tggatcaacg
    27061 ttacattatt ttctttaaac cctttgatca tttcgttgac acattctgta ctatttttta
    27121 agaaaagctg ataagccacc attttatctt ctctcgaacg gtgaacttga tcgaccaaat
    27181 cagaagtaga ggcagggcta cccgtccaac caatcaataa acgtaaatct tcaggagcaa
    27241 tcaatggctc aatggataga cctggccaat ccagggcaag taactcactg atagaatgct
    27301 gagtttcttg ttcttggagc caaggatggt cgaaggttga gaaagcgatc cagccaccat
    27361 aacagctagc agcgatgtcg ccgcaggaac cattatcttg aactgctaaa ttggctagtg
    27421 ctgcaatctt gaaaatctcc aactgagata aattcaacgc gtaaaaaaca ttcaaggctt
    27481 tgaccgttgc aacggtcacg gcaccgctag aacccaatcc atatttccgg ccatttgaac
    27541 tgtctaactc acttgttact ttcagatcat aaaaggataa aagaatgttt ttttcttgtg
    27601 catacttttc agtcaagcga atagcagcaa ggatataatg aaaaggattt tcccgaatat
    27661 ctaaaactaa ttctccgttg cgtcttgtcc aacgtacagg catcccacta tattgtgcag
    27721 attggatact tccgactttt cgtgcagatt ctacagtgac tgtcacgaat tgatcgactg
    27781 cagcgataac tgctggatgg cctgtttcaa caactgcata ttctccggca atatagagtt
    27841 tgcctggtgc agatacttca atcataaaaa tctcccttta ttcaataatc gcaattcctg
    27901 gaccagcatg tgcagtaatt acttgttctt tgctaaataa tttggtaaat gtttcttgta
    27961 cctcagataa atggctgttt tccactaaaa ctttgacatt agggccagca tccatcgtaa
    28021 agtagcatgg aatgccttgc tttctcaatt gtcgcaccgc atccatcgct tttaagctgt
    28081 caggagacca gtaagtgaat ggaggctggg cagctaaagt cgttccatgc atttttagtc
    28141 cgtttctttc catcgtttct ccgagaagtt ggaaatcttt tgttttgatt gcttgtttca
    28201 attgatatag atccccttca acggaatcta accagccttg ataaaaactg gatgtttcga
    28261 ctgtccgacg catcccattt ctgctggaca cttctttttt ctggtcgttg atcaaaacga
    28321 aaaccattgc taaatcgtct tcgaaagaat cggaagggac tggcttagcg taagaactta
    28381 agtcgtcatg gcctttttcc cattcgacga aaccaccgaa aatacttcgg caagctgaac
    28441 cagacccgcg tcgagcaaat cttgaaagag agagatcatc taatccaagc ttaagagcag
    28501 tattgcaagc gcctgctaaa gcagctagcc cgctggcaga ggaggctaat ccagctgcgg
    28561 taggaacgaa attctgactg attactgaag cttttagtga gaggccggct tctttacgaa
    28621 aaaggtcaag aaactgactg actttttttg ttgctttttc gtcttgcaag gtgccatcta
    28681 gataaaattc atctaccata tagctatcag aaaagatgac ttctgtctct gtataaaatg
    28741 catccaatgt taacgaaagg ctgttgttca ttggaaggat gagttcttca tttttcttac
    28801 cccaatattt gattagagca atgttggtat atgcgcgtgc tttgccttta aacataggta
    28861 tgtactccta atccttgtat ccaagtttca gcagcacccg catcttcaag tgcttgactg
    28921 atttcttggg cttttgtttt tgtttgtgct aatgcaatca tacagccacc gcgtccaccg
    28981 ccagttaatt tggcacccaa tgccccggta tcttttgctg tttggatcaa tagattaaga
    29041 aaatcattgc tgattgtcag cttttccaga agtgattgtg attcatccat tgtctgtgcc
    29101 aacgtttctg gtgaattttc aatgattgct tgttttgctt gcttcgtcaa gtatcctaat
    29161 ttttggatca tttgtccagt ttcatgaggc tgggtttcaa agaggtgagc gacatctttg
    29221 acggcttctc ttgtttgtcc tttgattcct gtatcagcga caatcaaaaa agcatcgatg
    29281 ttcaaagaaa agtagtcgaa aggatgccct tttgtaaaat aaatcggctg caagctgcta
    29341 gtagcggctg catcgattcc actaggatta ccgtgggcga ttttttccga aagctggaca
    29401 ttttctaata gtatttcacg agacaaagga aatgctaagt aatcataaaa agcacgagtg
    29461 actgctgtgg cgactgcagc gcttgatccc attccacgtt cagcaggaat cgtgctttca
    29521 atggtcagtt gcaagttttt ttggatgtca tgctgttttt ttaagtgaat gaataatttt
    29581 ttgatatttt ttaatgcatg aggagcttcg gctaaatttc ctgaatagta ggaagaaacc
    29641 agttgatcgt ccattgcatc cagctcttca aggaatgcgg tgacttttgt tgcatagaaa
    29701 ggaaaggcaa tcgctggttc tccataaaca accgcgtgct cgcccatcaa tatgatcttt
    29761 ccgcttgatt ctccttggcc atagtttgcc ataatctaac tccttctcac gtaaaagtga
    29821 tttttaagca aaaaataaag cgcccaaaac gagttgcgct gcttcacgaa tttttttgtc
    29881 cactaagggt ctcaaaaaac attcctcctt tattttacta aaaccatctc agaagtaaaa
    29941 ctaagaatac gttttctaaa gaaaaaacac ttttttctta agaatttttt gacttttttt
    30001 cttctttgac tagaaaaaaa tacaattgac actactagat attgggggta tatttaaaaa
    30061 ggttggagtt aaacacaata tttagtgatg aagtgagctg gaggtaaatg agcgtgatcg
    30121 ttttagcagg tacaattggt gccggaaaat caagtttaac agaaatgatg gctgaacact
    30181 ttgatagtca ggcattttat gaatcgattg atgataatga agtcttgccc ttattttatg
    30241 caaaccctga acagtatgct tttttgctgc aaatttattt tttgaataaa cgttttgcaa
    30301 gcatcaaaca agcaatgaaa gatgacaata atgtattgga ccgttcgatt tatgaagatt
    30361 cactgctttt ccatctaaat gcagatctag gaagagcaac agaaacagaa gttcgtgtgt
    30421 atgatgaact gctggaaaac atgatggaag aacttcccta tgctgcccat aaaaaacatc
    30481 cggatttatt agttcatatt cgtgtatctt ttgatacgat gttggagcgt atcgaaaaac
    30541 gtggtcgttc gtatgaacaa ttgtcctttg atccaagtct atacgattat tacaaagaat
    30601 tgaacagacg ttacgatcaa tggtatgaag aatacaaaga aagtccaaaa atccaaatcg
    30661 atggagaccg ctataatttt gtggaagatc ctcaagcaaa agaagaagtc ttgaaaatga
    30721 tcgaagaaaa attagcagag attcgccaaa ccaaagtagc catttgattt acagttgctc
    30781 aaataaaggg taaaccttta tttgagcgac ttttttcttt atactggaaa caaatgtcag
    30841 gaggatagag tatgcgggaa aaacactatt ttaatcaaga ccgtaatttt tggatcaatg
    30901 tacagcaaaa cgatgtgcta gctgttaaaa acttaaaaga agaatatggc attagtgacg
    30961 agatgctgac ttattctcta gataaaaatg aacgggcgcg cgtagagtac gattcttttg
    31021 atgaagcgtt actgcttgta tcaaatgttc cgcaccagca aaaagtcgat aatcattatg
    31081 aaactagtcc tatcgctttt attttaaaag aagacggttt gtttacattt accacgccga
    31141 ataccgagta cgtgattcga ctaattcgat cgcttttgga acgaatgcca gacatgagtg
    31201 tatacagttt attgtttcgt acattgtttt tgatttctga ttcctttttt cctttaatcg
    31261 aggaagtgaa tagtgaacgt cagcggctta atctgaaatt aagagaaaaa acaacgaaca
    31321 agaatctgct gcaactttca gatttagaga tcggtttggt ttacttagtt acaggaacaa
    31381 aacaaaatgt agtactttta gaacaaatca aggcattggc tatttatcgc aagttgtcag
    31441 aaaaggaaaa agaagagttg gacgatgcgc tgatcgaagc acgacaagca gttgaaatga
    31501 cgaacttagc ttcgcagatt ttagaccagc tctctggcac ttataataat ttgctgaata
    31561 acaacttgaa tgacacgatg aaatttttga cggtctggtc gctgatcttg accgttccaa
    31621 ctattgtgac aggattcttc ggaatgaatc tccagttacc ctttacccat tccgttttcg
    31681 gatggggaat cgccttgatc atcagtttgg tcttatctat ttggatgctt attgcactgt
    31741 ggcggcggat tcgttgatct agtctttgat ctgcaaacca agggcttggg cgatcacaag
    31801 tgcttgctct tggttgacga ttagtcccgc caaagaatct tgagataaag cgattgctgt
    31861 gaagaaatta gtcgtaaagt ctaatccctt cagagaagtt cgaaaccagt tcgaaccggt
    31921 caactgattg tcagacaaaa aaaggttttt ccaagtgacc tcataaaatt cgctgtcttc
    31981 taattgacaa tgatcgaagg agacagcttt caagtttgta ctactaaagg aagccatcga
    32041 agcaatacaa tcagtgaaag tacagtctcg aagataactt tcggcaaaat tcgtacctgt
    32101 taatttacat tgatgaaaca cgacttggtg gaaactgcca ccaatccact ccagattgga
    32161 aaaatcacat ttttcaaaaa tgacattact gcattcaaaa tgacgtaaaa ctgttttttg
    32221 catcgtaagt ttttggatat gactctctct taataccata tttttggcat cgagatagct
    32281 ttggtcaccc ccgttgatca gaagaccaga gaagatctcc tcatcttcta aataaaactc
    32341 tccctttgta agaagaggaa gagaaggtgg aataggttcg atttttttca tgtgggttca
    32401 cctcgttttt ctttagtata gcaaagaagc gggaatcact gctagaaagt ctgctaatct
    32461 aatcattttc agaaaaggaa gacaaactgt atgaaaaaca ttgacaaaaa gattagtttt
    32521 tgctatacta tgatggttga gaaataaaag cagaagcacc cgcttctcgc cttagttgat
    32581 aatgtcactg ggctagatag ttttcactgg ttctattagg tgaagtacgc gcgggttctg
    32641 taagagaact cgttttttta ttgccgttta ttcggtaagc ttttctttct ctcataatat
    32701 ttggaggtga atgaccatag caaaggatat gatggttaac gacggcattc gtgcacgaga
    32761 gttacgattg atcggttcag atggtgagca attaggagtt aagacaaaag cagaagcatt
    32821 gaatattgca gaacaagcaa acttggatct tgtgttagtt gcccctggtg cgaaaccacc
    32881 agttgcgcga atcatggact acggtaaata ccgtttcgaa caacaaaaga aagaccgcga
    32941 agcgcgtaaa aaacaaaaag tgatcaatgt aaaagaagtt cgcttaagtc ctacgatcga
    33001 cttgaatgac ttcaatacaa aacttcgtaa tgcacgtaag ttcttggaaa agggcgataa
    33061 agtgaaagct tctatccgtt tcaaaggccg tgccattacc cataaagaaa ttggtcaaaa
    33121 agtcttgaat cgcttagctg aagaaactgc ggatattgca acagtggaac aaaaagcgaa
    33181 aatggacgga cgcagcatgt tcttgacgct ggcaccgaaa aacgacagta agtaatttga
    33241 atgactaact ggctgttgga cagccgatag tcggataacg aaagattttg aggaggaaat
    33301 atagtcatgc caaaacaaaa aacacaccgc ggatcagcaa aacgtttcaa acgtactggt
    33361 aaaggcggat tgaaacgctt ccgcgcgttt acaagtcacc gtttccacgg caaaacaaaa
    33421 aaaacaacgc cgtcaattgc gtaaagcaag catggtttct agtggcgact tcaaacgtat
    33481 tcgccaacaa ctagcaaaaa tgaaataaga agtacaaata acagaatctg aagatttatt
    33541 tttattagga ggaattatac atggcacgtg ttaaaggtgg aacagtaact cgtaaacgtc
    33601 gtaaaagagt gctaaaatta gcaaaaggtt actacggttc aaaacacact ttatttaaaa
    33661 cagcaaaaga acaagtaatg aattcataca actacgcata tcgcgatcgt cgtcaaaaga
    33721 aacgcgactt ccgtaaattg tggattgctc gtatcaacgc agcagctcgt atgaatggct
    33781 tgagctactc aaaattaatg cacggtttga aagttgctga aatcgacatc aaccgtaaaa
    33841 tgttagctga cttggctgtt aacgatgcag cagcatttac tgcattggct gaccaagcaa
    33901 aagaagcttt ggctaaataa gctaaacaat tataaagagg aaagaagata acgaaaagtt
    33961 atgttctttc ctcttttttt atttacaatc ctccatcaaa tgaaacagga caataacgta
    34021 taagtctaaa atagcataat taaggttttt taattgaaaa ctgaacattt atggtcaccc
    34081 tttaatgaaa gcgctaacta cataagttag aatgagtttg actgatatat tagtttgctt
    34141 atcagaaaaa caaaatggac ggaggaataa catgagtgat attattattg gtattttgtt
    34201 agttatctca ttctttttca tggtctggta ctgtgtaaaa ggctacaact tgatggtcgg
    34261 ctttgccatc atggctactg tatggatggg attagcgcta gtcggaaaca cgttttcacc
    34321 aaacccggcg atggaagggc aaggagtaat tgatgtgttg acgcatatct atacgacggg
    34381 gccagctgag tacgccaaat cgatcttggt aaatgtcttc tttggtgcct ttttcgggcg
    34441 tgttctagta gatagcggaa tcgcagctac cctgattcga aaggtagttg aacttggagg
    34501 ggacaagcca agaatcacga tgagcttact atgtattgta accgctgtta tattcatgtc
    34561 gatgacagga atcggaccag tgatttctat tgcagttatt gtgttgccaa tcttgatgtc
    34621 attaggtatt tcagtaccgg ttgcactgtt ctcatttatg ggctcgatta tggctggaat
    34681 ctttgctaat atcgtcaatt tcaaacaata tcaaacgatt tatgctggtt tcaatccagc
    34741 ggctgaaagc tatacttata atgactattt ccaaattggt atgattggga tgattgtaag
    34801 cttagttgtg gtactaacgg tcgctaatat atcaatgaac aaaaagaaac gctatgcaat
    34861 ggcagcaaac gtaccagctg aaggtggcga cgcaccaatg atctcatggt tagccgtatt
    34921 attaccagtt ttaggggtcg ttcttttaga tcttccaatc atcttaggtt ttattctagc
    34981 aggtatctgg gcattgctgt ttactggaaa attgcgcgga ggttataaag aaatctgtcg
    35041 tcagtttgcg aaattattta cagatggcgc agtagatgtt gcaccaatgg ttggtttctt
    35101 gatgacctta gcgatgttca ataattcagc tgcgtatgct tctccttatt tttcagctat
    35161 ttttggagat tggataccgc aatcaccatt agtcttggca atcgtatttg ctatattgac
    35221 accactagga tttttccgcg gtccaatgaa tctagtcggt tcaggctcag ctattttagc
    35281 tgtcgtatta gctgttaatc caacgatgtc gccagccttt ttgttcccgt tatttgcaat
    35341 cacaacgatt gctcctcagc atttagatat tacacaatct tgggttgctt ggggattagg
    35401 ttatacaaaa gtaacttctc gagaatatat gaagaaatcc atcccaactg gttggatcat
    35461 cggagcgatc ttatgcctga tcacgttctt attgtacggg aacgcctagt aaacaaaacg
    35521 tcgataaaaa cgattagatg aatggaggaa aagaaaatga aacgttttgt gcgaatggga
    35581 attgatgttg gcggtacaca tacgaaagca gtcgcaatcg ataatgcgac gcacgaaatc
    35641 atcggaaaat cttcagtgaa aacaacccac gatgatgtcc gtggagtagc cgcaggtgtc
    35701 gttcagtctt ttcaaaattg tttgcgggaa aataatatca gtccagaaga tgttgtgttt
    35761 gttgcacatt ctaccacaca agcgactaat gcactgattg aaggagacgt agcaaaagtc
    35821 ggcgtaatcg ggatggcaaa aggtggatta gaaggttttt tagcaaaacg acaaactaga
    35881 ttgaacgata tcgatttagg gaataagaaa aaaatagaga ttgtcaatgc attcttacct
    35941 gtcaaacact taaatgtaga ccgtgtttca gaaacgattt cttctttaga aagagaaaga
    36001 gcggaggtct tggtttcttc tatggcattt ggagtagata atggggaacc agaacgagtt
    36061 gtttatgaag cagcatcggt aaaatcgatc cctacaacga tggcttctga catcacaaaa
    36121 ttatatggat tgacaagaag aactcggaca gcagcaatca atgcatctat tttaccaaaa
    36181 atgctagata cggcaacctc tacagaagat tccgtacgag aagcaggggt caatgtttct
    36241 ttgatgatta tgcgcggaga cggcggagta atggaaatca atgaaatgaa aaaacgacct
    36301 gttttaacca tgctttccgg tccagcagca tcagtcatgg gctctttgat gtacttgaga
    36361 gcgtctaatg gtgtttattt tgaagtaggc ggaacaacaa ccaatatcgg ggtcattaaa
    36421 aatggacgtc cagcaatcga ttattcaatt gtaggtggcc acccaacata tatctcttca
    36481 ttagatgttc gggttctagg tgtggcaggc ggttcgatgg tacgggctaa tcagtcagga
    36541 atcatcgatg tcggtccacg ttctgcacat attgccggtc tagattatgc agtatttacg
    36601 gagacagaaa aaatcaaagg tcctaaggtt gaatttttct ctcctaaaga aggagaccct
    36661 gctgattacg tcaaggtagt gatggaagac ggagaagaag tgactattac aaatacatgt
    36721 gcagccaatg tccttggatt agtgcaagaa gaacattttt cttatggaaa tgtcccatct
    36781 gcacgaaaag ccatccaagc attagctgat tattgtcata cgacggttga agacatcgca
    36841 gaacagatca tggaaaaatc ctatgctaag attgaaccgg tgattttaga attggcagac
    36901 aagtatcact tggagaaaga tcagatttct cttgtaggtg tagggggcgg cgctgcctca
    36961 ttgattactt actttagtaa taaaatgggt gtgaaatatt cgattcctga aaacgccgaa
    37021 gtgatttctt ctattggtgt agcacttgca atggtccgag atgttgtaga acgtatcatc
    37081 ccttcgccaa gtaaagaaga tatacgatcg ttgaaaaatg aagcgatgaa taaagcgatt
    37141 gaatcaggcg ctacaccaga atcgatcgaa gtccacgtag aaatcgatcc acagacttca
    37201 aaagtaacgg ctatcgctac tggttctacg gaagtgaaag caaccgatct aaccaaggag
    37261 atcacaacag aagaagcact agagcttgct gcagaggata tgcgattaaa taaaaatgaa
    37321 gtttgcttat tggaaaatac acctttcttt tatgtatgcg gcgaacagaa tcgttcaaaa
    37381 aatgctggaa gtcttcggat catcgatcaa aaaggattta tcaaggtcca acgtggacat
    37441 gcttcatgta tgaaaacaac tgcagccaat tatatgacag ctgtcgaaca actttgggaa
    37501 gatatggcag tttatcaaac tgaattgatt gcccgaccag aattttatct ctgcttagga
    37561 gcaagagtct ctgattttac agcgacggat ctggaacaac tacagctttt gatggatctg
    37621 gaagtatcta ctatggaacc ggaggaagaa gtgatcgtcg ttgccggaaa tatcaaacaa
    37681 acataagtta gaagagttac taaacgaatt acaacagatc cccgaagaaa tttggggatt
    37741 ctaccagttt cggcgagatt tattttggaa aaagattcct ttgtccaagc agaaaattct
    37801 tatacaacaa tcaatcgatt gtggtatcga aacggcatgt agcatcaaaa aaaagtaccc
    37861 ttttgccgat gtaggagaaa tttgtgaaca aatggctgtt ccaatcgttt cctgtgaatc
    37921 agagcaaata aacgaacgaa tcacttttgc aacttatgca gaagatgaag ggattcgatt
    37981 gatgacggag ccgttagaaa aattaaaatg ttcaggacta gcttcgatct ctaaagaaac
    38041 agcgcaagct ttgattattg gacatgaact atttcaccac atcgaagctt ctgttaaggg
    38101 aatttatacg caaagcgaaa aaattgtgct gtggcggttg cctttttata cacatcaatc
    38161 aaacattcgt gcattgagcg agatagcagc gatgagtttt tcaaaagaga tgaatcaaag
    38221 cgatttttcg ccttatgtat tagaggcagt cttactttgg ccatataatg aaattcgtag
    38281 ccaagggatc ttagaagaaa taaaaaaaat agaaaaaaga tgtacggagt atgatttaga
    38341 ttacaaataa agagactgtg acaaaagtcg tgtcacagtc ctttttttcc gaataaacga
    38401 tgttctcctg tcaactcctc ggtcacaagt caaaatattc aacaaacatg agaagctgtt
    38461 ttttgaaatt gttccttgtg actcggagat gaacggcagg ctcacgacct cttggaaaac
    38521 ggtgttcaaa agcttgcatc tgcggtaata agtccagcca agttaaaaac ataagaagct
    38581 atttttactt ggctgttctt attacttgat gcagggcagc tttttcacaa cctctcaata
    38641 aacggtgtgc aatcagctga ccctcggtat tagctcaaaa aaaacggcaa aatatgagga
    38701 actattttgc cgtttctttc tctaatactc aggtcaaaac gctgattcca aaacctctta
    38761 tttgatgggt ctgtcctgaa tatcttcaaa ataatcgggg taattatgaa tcacgtattc
    38821 ttgctcctcg ataatcatat gtaaatgcat cgtgcaaaga aatccgactt catcaaagtt
    38881 atgatcaatc attgattgaa gcattgtctg atgttcatct aatactcgac cccagtctaa
    38941 ttctgaaatc gtcaaacgta accatctgaa acggttcaag tgcgtactat aactttctag
    39001 ccaatcccaa atttctcgtt ttccagcaat ttcataacaa gttcggtgaa aatcagtatc
    39061 caaatcaaaa aaagcttttt tattttgagc gattactgcc tgatcggttt ttccaacttt
    39121 tcccgcgtat aacgggctaa tgaagcagat cggatatcga tttttgaaac ataagtgccg
    39181 gactgtggaa tggtttttac aagctcttga ttgcgcagtt ggatcagtgc ttctcgaatc
    39241 ggtgtacgcc cgatagctag ttgttcttct aacactttat cggagatttt acttccaggt
    39301 gctaactcgg aataaatgat ttgtttacgt atagattcat aagcttgctg ttgcaaatta
    39361 ttgataacca tttaaccttc ctttctatgc agatataatc tattataaca taaatcgctt
    39421 acaaatcgtg tgggcataaa aacacagtct gcaaaaaaag ccctgatagg catgcctatt
    39481 agagcctcga aaagaaaaat attgaatcaa tctggtcgta cctcttgtat cggtttgaca
    39541 cgaccgccga ttcttttagc aaaagcttcg gattcttttt tgtctttgaa aatgagcaga
    39601 ttgtctaagt ttttttcccg agtgcgacag ccagttttat cgatgtaacc gtgcattact
    39661 ttaacgacat acatggggat cacctgcttt tttcttttca ttatacgaac tactcgacac
    39721 aatacaagaa aaatgcttag acggtcttta ttaagaattt acatcatcca aagaagcgta
    39781 cgcttggaat aactgatcta actccggatt gactttgctc agcttcacta atcgttgcga
    39841 atcagaaagt aaaaaacagt gtttactact gccagtagtt tttaattttt tgtcttcagt
    39901 tcccacaatt tcgtagctaa aatccaaccg tgtccccgta tacttttgga tagttggatg
    39961 gatgctcact ttttctccgt aatggatcat ttctttatag ctgcaattta cttccagaac
    40021 ggggatgatg atcccagatt cttcgatttt tttataggga aaccctagat gtaccaataa
    40081 ggctacccga gcctcttcaa accagcggat ataattgctg tgatgaataa tccccatttg
    40141 atctgtttca taataaaaag gttctcgcac ataacctgta aatttctcca tgactcatgc
    40201 ctcgttttcc attgattttt tacaaaaata attcatccat ttcggaagtg cttcgctgat
    40261 ttgtgtcggc aaaacaatat attgttcttt tgccagctgg tctccaatga aactatgaag
    40321 ataaatagct gctgcaatcg tttggtcatt ttgaggaaac tgggctaaaa atcctgtgat
    40381 catcccagct aaagtgtcac ccatgccgcc cgttgccata gcagcagaac ctaaaggatt
    40441 ggtgtagaag tgctggtgac cataaatttt tgtctcatga cttttcaaaa cgagcacagc
    40501 accgatttcc tgttgcttct ttttgttttc tttatcccgt tgttcttgta tcggtagaga
    40561 actcaaccgt tgccactcca tttgatgagg tgtaagtaca gtttgttcgg gatagggtag
    40621 caaaagatga ttagcagcaa gtaaacttaa agcagagcca tcaatcacca gccattgttc
    40681 ttttgattgg ttttctagta cttgtttgaa acgttccaaa ctcttagaag aagtacccaa
    40741 tccgggacca atcaagagta catctgcttg ctttattacg gaaagaaact ggtcagtatc
    40801 cgtccaaact acaatcattg cttctggcag atgactgtga agagcactcc gatttttttc
    40861 atcagttact acagtcgtga gacccgctcc gctgtgtacg gcagctaaag cactcataat
    40921 gattgctccg ccatattgtt cattgccgcc aatcaaaacg atacgtccaa atgttccttt
    40981 atgtgaaatt tttggacgag gtgtgatgac tcttttcact agttgttcgt ttatcagcat
    41041 ctaaaaacct ccttttactc attatagcgt atattttttt tagagaaaaa cagataagaa
    41101 aaggaattgt cagatttttt gaactatttt tttctgtaat ttgtggtatc atgaacacga
    41161 agacttaaat aagtggagga ttaactttat gacaattgat tggcaaaaag aagtagaagc
    41221 acgtaaagaa gacttgcttg aagacttgaa aaatctttta cgtgtcaaca gcgagcgcga
    41281 tgactcaaaa gtaacaccag atgcaccatt tggacctgga cctcgcgacg cattgaaaca
    41341 tatgttagct tatggcgaac gtgatggatt taaagtaaaa aatgtcgata actatgcagg
    41401 gcatattgat cttggcgaag gagacgaaac gctagggata tttggacaca tggatgtcgt
    41461 tcctgcagga gatggctggg acacagatcc ttatgaacca gtaatcaagg atggaaagat
    41521 ctttgcacgc ggatcaagcg atgacaaagg tccaagtatg gcagcttact atgcgatgaa
    41581 gatcattaaa gaacttgact tgaaactatc aaaaaaagtt cgcttcgtag tcggcagcga
    41641 tgaagaaagc ggctggggcg atatggctta ttatttcgaa catgaagaag aaccagattt
    41701 tggtttctca ccagatgctg aattcccaat catcaatggt gaaaaaggaa atgtttcttt
    41761 agctttacgc ttcaaaggcg acaatgcagg agattatgtc ttgaaatctt ttgtttccgg
    41821 acttcgcgaa aatatggtgc caggaacagc aactgcagca cttcaagtac caagtgcaga
    41881 tgcagctatc gcgatggaag aagcatttta ccaattcatc gaagcaaatc cagtaagcgg
    41941 aacgattgaa gcggacaata cgtatgtaaa aatcgaatta gttggtaaag gcgcacatgg
    42001 tgcaagccca caatctggta tcaatgcagg ttctttcttg gcattgttct tagataacta
    42061 tgaattccta ggatctgcta aacaatttat ccatgtagca gctgcttatg tccatgaaga
    42121 tttttatgga gaaaaactag gagtcgctta tgaagatgaa aaaatgggca aattgacaat
    42181 gaatgctgga ctatttgctt ttgaagaaaa tggtacagag gaagcgaact tcatcaatat
    42241 gaatttccgt tttccaaaag gtgtaacagt agacggattg caaagtgaca tcgaacaaac
    42301 agtggggcaa gaaggcgcaa cagttactcg aggtgctcgc gtaatggaac cacattacgt
    42361 accaatggat gatccattgg tggctacatt attgcaagtc tatgaagacc acactggaga
    42421 aaaaggctac gaacaaatca tcggtggcgg aacttacgga cgtttgttga aacgtggcgt
    42481 tgcttatgga gcgatgttcc caggatatac tgatacaatg catcaagcaa acgaatttat
    42541 gagtcttgat gacttgttca gagcaacagc aatctatgca gatgccatct atcgtttggc
    42601 gaaataatta attaaaaaga ggatcaagaa ttgcaaatta tttttgcagt cttgatcctt
    42661 tttttacttt ctactgtggt attcatgata aacatgatat agattcaatt catattcttt
    42721 cttagcgttc gtgacgactg tatccaaaat caaccctgtt actaatgaaa gaagtgctaa
    42781 agtcatcaat ccagtactta aaatcgcaga agggatgcgt gtaataaagc ttgtcagcat
    42841 atattcgcga atcacaggaa tccccgtgac caatccaaat aaaaagaaga agatggtcca
    42901 aatcccaaag aaaagcaaag gcttataatc tttaaacatt ttaataatca tcatgatcac
    42961 tttgaatccg tcagagaatg tattcaattt tgattcgctg ccttcaggac gatcacgata
    43021 atcgattggg atttcaacga acttgaattt tttatctaaa gcatggatcg tcaattccgt
    43081 ttcgatttga aaaccagaac tcataatagg aaaacttttg acaaagatac gattgaatcc
    43141 tcgatagccg gtcatgacat caagaatttt tgttttatac attctagtaa ttgtatttct
    43201 aacaaggtta tttccaaaat cgtggaaagg tctcttattc tcatcaaagt aggttccatt
    43261 tgataggcga tcaccaatga ccatatctgc ttcaccagaa cgtaatttct cgagcaaacc
    43321 atgtacagct tctgcaggat aagtatcatc gccatctacc attaaataat agtcggcatc
    43381 gatatcaaag aacatttggc ggatcacatt tccttttcct tgacgaggtt ctttttttac
    43441 gattgctcca ccttgaacgg ccaattcgta tgttcgatcg gtcgaattat tatcatagac
    43501 ataaatatct gcttctggta attctcgttt aaagtctgca atgactgttg aaattgttgc
    43561 ttcctcattg taacaaggaa tcagaacagc aattttttct tgttgttcca tttttcactc
    43621 tccttaacta tagaaaacaa aagctaaatt aaaaatacct cataatcaaa ggaaatgcta
    43681 gtttttcccg ctaaatatca tttatttaaa aagaaaatac tggaatatat gtttggaaag
    43741 ctctatctaa aaaaccaccc actacaaaag aaggacatga tacctctctt ttgaatagtg
    43801 ggtgcgtatt cgttctctca cttttttgac gtattaatct gttgcacctt tttttcttga
    43861 aatttctaat acggttcctg tatgagcatc tgctaaaaat tcataataaa cagtttcgcc
    43921 atcttctatt cgagaaagac ctccccgata agcactcgac cgaacggcga atttcctaat
    43981 aggttccttt tcaaaagaga tccaagaacc ttcgattgca ccttctttta gaaaagatga
    44041 cttgatattt tcaagtactt ggtctgctga caatactttt ttgcgatgat accaaatcgt
    44101 tgaagctacc ccgctcaata atcctagacc gattccaact gctaacccgc ctttaaaata
    44161 gcttagttcg ctttcttcat acatgtggac atacctcctt cttctcactt gttactcctt
    44221 gtgcttattt taccacactc tcatattttc tttgaaataa ataaccgcta ccagaaaaaa
    44281 taagctataa taaatacgat cggtctgcag aaaaaatcat attttatccg cagatcaata
    44341 taagtctgag aggagtttag tctgaaattt agacgaatag catatggaag aaaaaacatt
    44401 tcaacgaatc aaagagttaa cagaattaca aggaacaagc ggttttgaac aagatgttcg
    44461 cgcttatatg gaaagtcata tggaaccgct ggtagatgag ttacagttag atggtttggg
    44521 cggcattttt ggtctgcgcc atcatgaaga agcggatgca ccccgtgtaa tggtcgcagc
    44581 acatatggat gaagtcggat tcatgctgac acaaatccaa gaaaacgggc tttttaaagt
    44641 cgtacctcta ggcggctgga atccttacgt tgtttctgct cagcgtttta cacttaaaac
    44701 ctctactggt aagaattatc catgtatttc ttcttccgtt cccccacatt tattgcgagg
    44761 aacaagcgga caaaaatcag tagaagtaac cgatattttg ttcgatgctg ggtttgaatc
    44821 aagagaagag gcaatgagtt acggtgtcct tccaggagac acaatcgttc cttacgctga
    44881 aacgatcaaa acagccaacg gaaagaatat tatcagtaaa tcatgggaca accgctatgg
    44941 ctgtacaatg gtattagaag ctttagaagc gttgcaaaat gaaacattag gtcatacatt
    45001 gattgcaggc gcgaatgtcc aggaagaagt aggcttgcgt ggttctaaag cttctgtcaa
    45061 taaattcaag ccagatctgt tctttgcagt agactgctct gcagcagatg atacagtgac
    45121 gaaaaatggc acattcggac atttaggaga aggtacattg atgcgtatcc aagatccagg
    45181 tttgatcatg cttcctcgtt tgcgcgagta cttgctggat atcgctgaaa caaacaatat
    45241 cccttatcaa tactttgttt caaaaggcgg tacagatgca ggtgctgctc atacacaaaa
    45301 cgaaggaatc ccaagtacag tgattggcgt agtgggacgt tatatccata cgcatcaaac
    45361 gatgttcagt attcgcgatt ttgaagctgc acgagaaatg ttgattcaaa cactgaaagg
    45421 tttggataaa tcaacagtga atacgatcgt atacggaaaa taatgcgata gtgaaactat
    45481 aaaatctggc aaaagattgc tttttgccag atttttttta taaaaagagg atcaatgttt
    45541 gttagttcga aagaaaggtg tataatttaa ttgaaaaata cataaacaga aagtagagga
    45601 caaagaatga ttatcccaaa tagtttagaa gaattagcag gctacgtaga aactggaaag
    45661 agtgtatttt tctttactgc cggctggtgc ggagactgcc gttttatcaa accgcaaatg
    45721 cctgaaattg aaaatgactt ttctgattgg caatttattg aagtagaccg tgacaagtac
    45781 atagatgtag ccgctgaatg gaacattttt ggtatcccaa gttttgtcgt gatccaagac
    45841 ggaaaagaat taggtcgtct agtgaataaa gatcgtaaaa caaaacagga aatcgaatcg
    45901 ttcctagaaa gtgtaacaaa aggctgacat ctgttaggag gggtcaagat gaatcaagaa
    45961 tataagaata ttttagtagg tatcgacggt agtgaacaag cacttgctgc tttcaaaaaa
    46021 gcggtagaag tggctcgccg taataatggg aaagtatatg tagcaaacgt aatcgatcag
    46081 caattttata actttatggg ctatgcacct attgatcaaa atctagtcga tcagcaaact
    46141 gaaacagcca aggaaatgat cgaagaatgt aaagaatatg ggaaatctgt tgattacaca
    46201 aatatagaag gaattattgc atatggttct gttcgtgaat ccatggcagt caaattgcca
    46261 aaaaaatatg agatcgatct aatcatggtt gggcagtcag gtctaaatgc agtcgaacgc
    46321 tttatgacag gaagtgtagc cagctacgtg atacgtcatt caccttgtga tgtcttgatc
    46381 gtttctgacg aagaagaaca gccaagtaat taagcaagtg gaacttgtac gaccttttta
    46441 atcaattaac taaaacagta aagggactgt gacaaaagtc ttgtcacagt cccttgttac
    46501 gaataaatgg tgttccagaa gaggcttctt cggaaataaa gcatgaaaaa aatttcatgc
    46561 agtgaatgca attttttgtt aagataaatc tcagcaaagg ataatggagg aaagaacatg
    46621 atttttgctt ataacaaagc acacgtcgga gacaccttga tggttattat agccgacgat
    46681 aaaggaacag aaaacactgt tgaacgcaaa tggaatgttg ctcggatcaa agatgaacaa
    46741 ggtcaagttg ttgcttggaa ctttttccat atctccgatc atctaacaat cgaagggaac
    46801 ggccaagtaa gcttaacaga tgaggaatta gcgaaattga atcgattgat caaagaagca
    46861 ggattcgaag aacagcttga agctgatcat gaaccgaaga tcgttgtcgg gtacgtaaaa
    46921 acatgcgtcc cacatcctga ttctgaccat ttatcgatta ctgagacaga agtagacaat
    46981 ggtacagtat tgcaaatcgt ttgcggtgca cccaatatcg aagctggaca aaaagttgtc
    47041 gttgcaaaac caggagctat gatgccagat ggaatgatga tctggccagg aactttgcga
    47101 ggggtagata gctatggaat gatctgttcc gctcgtgaat tgcatttgcc aaatgctcca
    47161 gcaaaaaaag gaatcttagt attgcctgag gatgccgtag tcggagaagc atttccaaaa
    47221 taaagaggtt gtgacaagtc tttgtcacaa cctctttttt attaattttc actattttga
    47281 ttttgtttta atgccgattg atcgatcgtc aattcaacag tagttgtttt ttctttcgag
    47341 ccttcatagt aagtgatttt gacagaatct cccacttttt tcttatacaa agcagattga
    47401 agttctacac cagaagaaac ttctttgtca tcgatcttag taatgacgtc gtattgtttc
    47461 aatccagctt tttcagccgg tgtagctgtt tggacatttg tcacgatgac accgttagtt
    47521 acagatgaag gaatcttcaa gatttgttct tgttgctgag tagaaacggc agataagtcg
    47581 accattgtga tacctagagc tggacgtgtc acttttccgt ctttttccag ttgattgatg
    47641 atattcacta catcgttact tggaatagca aatcccattc cttctacgct tacattagaa
    47701 gtagattcag aagtacttgc gatcttactt gagttgatac cgataacttg tccttcgata
    47761 ttaactaaag gaccaccaga atttccaggg ttgattgctg catctgtttg gatcgcattg
    47821 atattgactg tttcgttcga ttcattcgta cttgtcactt gtcggttcaa ggaagaaatg
    47881 attcctgaag tgacagaatt tgcatattca gatcctagtg gggaaccaat cgcaatcgca
    47941 ggctcaccaa ccttcagagc acttgagtca ccaaaagagg caactgtttc gactttgtct
    48001 gaattgatct tcaacaccgc taaatctgaa taagcatctg ttccaactaa ttcagctttt
    48061 accttcgtac cgtctttcat caagacttca agaccttgct ggccatctac tacgtgatta
    48121 ttcgtgacta cataagcagt attcccgtct tttttgtaga tcacgccgct tccttcacta
    48181 taagcttcca aattactatc atctgagctg ctactttctt cttgctgccc gaatagtccg
    48241 ccaaaaccgc ttgattggtt ttgactttgt aagttgataa cagaaacgac agcgtcttga
    48301 actttatcta ccgctttggt gatatcagaa tctacattga ctttgacatt ttccacaact
    48361 gtttcaccgg cagaattctg attgccgctt gtagcagtag aactattgtt gccagtgccc
    48421 atcgctgcat aaaaaatgcc agctgtcagt agtcctccaa cgatccctcc aacaagcccc
    48481 agacctaact tacgccagag gctattattt ttactttttt tcatctttgg tgtcacattt
    48541 tttctatcca tgtgttcctc ctccaattac attcatttat ctactgttag tatagtctta
    48601 tttgttgact ttagtctact ttgagactgt gaacttttta tgaaaaactt taggaaaaat
    48661 cattgttttc agatgaaaat atttgatttt ctttttccac agatactgtg taaaactacg
    48721 cggataggat tggaaaaata agggataaaa catatccaca gcctgtgtat aaagtggata
    48781 aattcttata aaacgtatgt ttgcctaatg aatgtgatga aataaggtaa aataacactg
    48841 ttttctgtgg ataaacctgt ggatgttgtg cataactgtt ggggaaatct ttttaacaaa
    48901 aatagccaaa ataaacatat agacgaaaaa attgattata ataaacaaag tgagcatgaa
    48961 gaccaaagga gagaaaacat gaatatcaaa attatctcag taggaaaatt aaaagaaaaa
    49021 tacctgatcc aaggtatcaa tgagtatgtc aaacggctga atgcctatgc aaaaatcgaa
    49081 ttgatcgaag ttcctgacga aaaagccccg gagaatttaa gcgaagcgca aatgagacaa
    49141 gtaaaagaaa aagaagggga acggatctta gcgaaaatca aagaacaaga atacgtctat
    49201 gccttagcaa ttgaagggaa aaatcctaca agtgaatctt ttgctaaaca aatcgaccaa
    49261 ttaggtatcc aaggaaaaag ccaccttgtt tttgttatcg gcggctcttt aggactcagc
    49321 gaagccgtca tgaaaagaag caatgctcag atctcttttg gaaaaatgac gtatccacat
    49381 caattgatgc gtctgatcct tgtcgagcaa atctaccgtg ccttccggat caatgcgggg
    49441 gctccgtatc ataaataatg atatttaaga ttgaatgctc ctgaacacta agttagcttt
    49501 tcattgaaa
//
LOCUS       DAYYAR010000006        48185 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000006 DAYYAR010000000
VERSION     DAYYAR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.006"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..439
                     /locus_tag="ACHAT0_000285"
     CDS             <1..439
                     /locus_tag="ACHAT0_000285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8450260.1"
                     /translation="RTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVRKA
                     LPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLTLT
                     VYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(922..1452)
                     /locus_tag="ACHAT0_000286"
     CDS             complement(922..1452)
                     /locus_tag="ACHAT0_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8450261.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            1557..2108
                     /locus_tag="ACHAT0_000287"
     CDS             1557..2108
                     /locus_tag="ACHAT0_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8450262.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(2261..2734)
                     /locus_tag="ACHAT0_000288"
     CDS             complement(2261..2734)
                     /locus_tag="ACHAT0_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8450263.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2872..4344
                     /locus_tag="ACHAT0_000289"
     CDS             2872..4344
                     /locus_tag="ACHAT0_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8450264.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            4341..5051
                     /locus_tag="ACHAT0_000290"
     CDS             4341..5051
                     /locus_tag="ACHAT0_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8450265.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            5403..5687
                     /locus_tag="ACHAT0_000291"
     CDS             5403..5687
                     /locus_tag="ACHAT0_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8450266.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(6004..6387)
                     /locus_tag="ACHAT0_000292"
     CDS             complement(6004..6387)
                     /locus_tag="ACHAT0_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8450267.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(6389..6973)
                     /locus_tag="ACHAT0_000293"
     CDS             complement(6389..6973)
                     /locus_tag="ACHAT0_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8450268.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(7020..8495)
                     /locus_tag="ACHAT0_000294"
     CDS             complement(7020..8495)
                     /locus_tag="ACHAT0_000294"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8450269.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            8676..8987
                     /locus_tag="ACHAT0_000295"
     CDS             8676..8987
                     /locus_tag="ACHAT0_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450270.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(9102..11438)
                     /locus_tag="ACHAT0_000296"
     CDS             complement(9102..11438)
                     /locus_tag="ACHAT0_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8450271.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            11580..12248
                     /locus_tag="ACHAT0_000297"
     CDS             11580..12248
                     /locus_tag="ACHAT0_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8450272.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            12251..13294
                     /locus_tag="ACHAT0_000298"
     CDS             12251..13294
                     /locus_tag="ACHAT0_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8450273.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(13531..14412)
                     /locus_tag="ACHAT0_000299"
     CDS             complement(13531..14412)
                     /locus_tag="ACHAT0_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8450274.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            14571..15299
                     /locus_tag="ACHAT0_000300"
     CDS             14571..15299
                     /locus_tag="ACHAT0_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8450275.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            15455..15772
                     /locus_tag="ACHAT0_000301"
     CDS             15455..15772
                     /locus_tag="ACHAT0_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8450276.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            15765..16373
                     /locus_tag="ACHAT0_000302"
     CDS             15765..16373
                     /locus_tag="ACHAT0_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8450277.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            16428..17219
                     /locus_tag="ACHAT0_000303"
     CDS             16428..17219
                     /locus_tag="ACHAT0_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8450278.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            17357..17845
                     /locus_tag="ACHAT0_000304"
     CDS             17357..17845
                     /locus_tag="ACHAT0_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8450279.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            17896..18768
                     /locus_tag="ACHAT0_000305"
     CDS             17896..18768
                     /locus_tag="ACHAT0_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8450280.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            18944..19306
                     /locus_tag="ACHAT0_000306"
     CDS             18944..19306
                     /locus_tag="ACHAT0_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8450281.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            19315..20067
                     /locus_tag="ACHAT0_000307"
     CDS             19315..20067
                     /locus_tag="ACHAT0_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450282.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            20081..21883
                     /locus_tag="ACHAT0_000308"
     CDS             20081..21883
                     /locus_tag="ACHAT0_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8450283.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            22160..23200
                     /locus_tag="ACHAT0_000309"
     CDS             22160..23200
                     /locus_tag="ACHAT0_000309"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8450284.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(23252..25396)
                     /locus_tag="ACHAT0_000310"
     CDS             complement(23252..25396)
                     /locus_tag="ACHAT0_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8450285.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(25753..27126)
                     /locus_tag="ACHAT0_000311"
     CDS             complement(25753..27126)
                     /locus_tag="ACHAT0_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8450286.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(27126..27521)
                     /gene="rbsD"
                     /locus_tag="ACHAT0_000312"
     CDS             complement(27126..27521)
                     /gene="rbsD"
                     /locus_tag="ACHAT0_000312"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8450287.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(27549..28466)
                     /gene="rbsK"
                     /locus_tag="ACHAT0_000313"
     CDS             complement(27549..28466)
                     /gene="rbsK"
                     /locus_tag="ACHAT0_000313"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8450288.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(28574..29587)
                     /locus_tag="ACHAT0_000314"
     CDS             complement(28574..29587)
                     /locus_tag="ACHAT0_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8450289.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(29668..30267)
                     /locus_tag="ACHAT0_000315"
     CDS             complement(29668..30267)
                     /locus_tag="ACHAT0_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8450290.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            30453..31154
                     /locus_tag="ACHAT0_000316"
     CDS             30453..31154
                     /locus_tag="ACHAT0_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450291.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            31164..33875
                     /locus_tag="ACHAT0_000317"
     CDS             31164..33875
                     /locus_tag="ACHAT0_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8450292.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            34326..35954
                     /gene="treC"
                     /locus_tag="ACHAT0_000318"
     CDS             34326..35954
                     /gene="treC"
                     /locus_tag="ACHAT0_000318"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8450293.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(36014..37195)
                     /locus_tag="ACHAT0_000319"
     CDS             complement(36014..37195)
                     /locus_tag="ACHAT0_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8450294.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            37447..38970
                     /locus_tag="ACHAT0_000320"
     CDS             37447..38970
                     /locus_tag="ACHAT0_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8450295.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(39039..39767)
                     /locus_tag="ACHAT0_000321"
     CDS             complement(39039..39767)
                     /locus_tag="ACHAT0_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8450296.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(39780..41060)
                     /locus_tag="ACHAT0_000322"
     CDS             complement(39780..41060)
                     /locus_tag="ACHAT0_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8450297.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(41201..41803)
                     /locus_tag="ACHAT0_000323"
     CDS             complement(41201..41803)
                     /locus_tag="ACHAT0_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8450298.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            42048..42254
                     /locus_tag="ACHAT0_000324"
     CDS             42048..42254
                     /locus_tag="ACHAT0_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450299.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            42566..43285
                     /locus_tag="ACHAT0_000325"
     CDS             42566..43285
                     /locus_tag="ACHAT0_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8450300.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(43366..44049)
                     /locus_tag="ACHAT0_000326"
     CDS             complement(43366..44049)
                     /locus_tag="ACHAT0_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8450301.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            44268..46454
                     /locus_tag="ACHAT0_000327"
     CDS             44268..46454
                     /locus_tag="ACHAT0_000327"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8450302.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            46800..47210
                     /locus_tag="ACHAT0_000328"
     CDS             46800..47210
                     /locus_tag="ACHAT0_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8450303.1"
                     /translation="MIKVYTSPSCTSCRKARAWLKANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            47224..47580
                     /locus_tag="ACHAT0_000329"
     CDS             47224..47580
                     /locus_tag="ACHAT0_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8450304.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        15679 a         8499 c         9590 g        14417 t
ORIGIN      
        1 ccgcacaaca acaaaattgt ctgccaaaga actggaatct gttctatcga tttggctgca
       61 tgctaacttg gattctcatg aattcattcc atcaacttat tgggagaatc aagtagaatc
      121 tgttaggaaa gctttaccag atgcagtgat ttacttagca gaaatagatc aagaaattat
      181 tggttttgct ggggtgattg atcagtttat cgcaggaatc tttgtgaaaa gggaataccg
      241 gcaacaaaga atcggtacgc aactaatgaa aaaaataaaa gaaaccaata aaacactgac
      301 tttgacagtc tatcaaaaaa atcaatcagc tattcatttc tatctttcac aaggttttac
      361 aattcaaaca cagcaaaaag atgaagcagt aaatgaggag gaacttgtga tgaaatggaa
      421 tcaaaacgct gatttttgat gaacagaata acgaaataat gctcccagcc taattttttg
      481 ctgggagcat tatttcattt tgaagtcact aatttaactg tgtgaatatg cagttgttac
      541 aatctttgat aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
      601 atatgaggaa ctatttttcc atttgtttct ctaatactca ggtcaaaacg ctgatttcac
      661 aacctcttga taaacggtgt tcaaacggct gcttctcggt aataagccca ctagcctaga
      721 aaatatgaaa agctattttc taactagcgt tcttattact caaagcataa cgctgatttc
      781 acaacctctt gataaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc
      841 aaaacatgag gaactatttt tccgtttctt tctctaatac tcaggtcaga acgctgattt
      901 cacaacctct ctttcacaac cttattttac tcgtttataa ttttcatcga agtattttcc
      961 gctacggata tcatctggca ttccatcgta tggttcttcg ctaataacat cttcattgtc
     1021 tatacctgca tcacccatat aagtgatcgt agcaaattca ggaacaagta attttggata
     1081 agtttcatat ttttcacgag ctgcttccat ttctgcgata tgttcgtttt ggaaagtaac
     1141 agtgatttct ggatgttctt caacccattt agggaaaaag atcattccgt gaagtttctt
     1201 ttcatttggc ataaagtcca atgccacgtc tgtccctgtt ggctctgtcc aagtgatttt
     1261 gaatacgccg tccgtcaatt taacgatatc agcttcttgg tcttttaccc aacggccttt
     1321 gaccattccg ccatggatac ggtaatcaac agtatggtca tttttggcat accattcata
     1381 ttcccaacca ttgtcatacg tatagataaa gtgagttcca ataaaatctt ccaattcttt
     1441 aaattctttc atgtttaagc atctccttat aaattaaaac atgtaataaa ctacatgttt
     1501 tcgattactc atttatgata gaatggattt aaagatttgt caaggaggga aaactgatgg
     1561 caagaaaaaa tacacttcga tatattttgt tagggttatt aagtaagaag aagatgacag
     1621 gatatgaatt gaatcaatcg ttcaaaaatg aaataggaga attttggcaa gcaaaacata
     1681 gtcagattta tcctgaactt gcaaaaatgg aagagcaagg aatcattcag catcaagtag
     1741 agatcacagg tgaaaaattg gaaaagaaag tttatgaatt aacagaagaa gggaaggaac
     1801 aattggcaga gtggattcat acacccacca atgaactacc ggttaatcga gacgaatttg
     1861 ttctgaaatt gtatttcgtc aaagacgtga atgatccggc attagtagaa attattcagc
     1921 agcagcgtga ccttcatgaa gaaaaacgac ttcatctatt agagcgaaaa aaaacgatct
     1981 ttcctactga aaaagaaaaa catgaaaatt acggacacta cctgatttta gcgcatgcga
     2041 tcaatcggga aacagaatat gcaaattggc taacagatgt attagaagaa agtaaccgaa
     2101 aaaaataaaa aaatatccga acagtgatgg agaattcctt tctatatgaa agaaacgcag
     2161 atcaccgttc ggatattctt atgttcaaac aggccttttt tcaagtattt atgtagcatc
     2221 tacttattaa aaagcttggt acttaggaga ttagtggcgt ttagagttct tttaacaact
     2281 gttcgtagtc agctttttca tcgacaggaa ggagcttgta agttaattcg tctgtctcgt
     2341 tgtcatgaat gatgtacatc ttagcattct cttctttgaa gttaccgatt tgtaaaatcg
     2401 tatagcgttc gtcgatcaat tcgatatctt tgattgcttt tgaaaaaact ttacggacat
     2461 ggtgcatttt cttttggcgg atttctgcct tttctgcagc ttttgacaaa gtaaatccgt
     2521 catctaaata ttttttgatc agttctactt ttatgactgt aggtaaacga tattttctag
     2581 gacaattcgc atcagcttcg atcgaacgaa tgaatccctt ttgttcccaa taccttaatt
     2641 gacgtggaga cacacctacg atatcactta attcactgat gccaacaacc aattgatcat
     2701 tttccaacaa acgtttaagg gaagccccaa ccatttttgt cactcctttt gtcctcttgc
     2761 ttcccttaat tatatccgta ttaacttatg tgtcaagtag gttgacaaat agcgaattta
     2821 ggactaaagt attctagtag tttcagaaaa aattcagaaa gaaggagaaa tatgagtaac
     2881 aaccaacccg tagatattta cggaaaacca tataatcgga cacttttagt tgttgtctta
     2941 ttaattggta cattttgtac cgttttgaat cagaccttat tgacgacagc ttttcctaca
     3001 ttgatgaaag ctttcgatat ttcagcgtct gatgtgcaat ggttaacgac tggtttcttg
     3061 ctagtcaacg ggatcatgat cccaataaca gcatggctga tcaataaatt cagttcaaga
     3121 aatttatatc tttctgccat gacgatcttt ttgatcggaa cgattacttg ctttacagca
     3181 cctaatttta gtacattact aatcggtcgt ttgatccaag cttgcggtgt gggagtatcg
     3241 atgccattat tgcaaaatat catgctatct attttcccgc cagaaaaaag aggatcagca
     3301 atggggatgt cagggatcgt tattggactt gctcctgctt taggccctac attatctggt
     3361 tatatcattg ataactacca ttggcgtgac ttgtttggta tggtactgcc aatcgttgtt
     3421 cttgtcttgg ttttagcatt cttcctaatg aaaagtgtta ttccattgtc aaatcctagc
     3481 attgatattt tatccgctat tttatcaacg attggattcg gtagtttact ttacggattt
     3541 tctagtgtag gagatgacgg ctggggaagc ttgaaagtaa ttggtttctt agttgttggt
     3601 gtgattttca tcgccttgtt ctcatggcgt caattacatt tggaacatcc attcttggaa
     3661 cttcgtgtat tcaaatcatc tacattcacg attgcagctg ttttagctgg tgtaacaaat
     3721 atggcgatgg taggggcaga aatggtcgta ccattgtata ttcaaaatat tcgtggagaa
     3781 tcagcattcc attctggctt gatgttgttg ccgggtgcat tgatcatggg ggctatgatg
     3841 cctgtgacgg gtgcgatttt cgataaatac ggcgcgaaac gtttagccat cacagggatg
     3901 ttcattttga cagcggcaac tgcaccattt gctttcttga caaaaacgac tccagtttta
     3961 tatatcgtga tcctttatgc gatccgtatg tttgggatct ccatggtcat gatgccggta
     4021 acgacttctg gtatgaatgc attgccaaat aacttgatca gtcatgggac agcggtaaat
     4081 aatacattcc gtcaaatcgc aagctcgatt ggtacagcta ttttgatcag tgtgttgacg
     4141 aatgtgacaa aagcaaatat cccaagcaaa tctgtattgg ataatacgcc attagcttat
     4201 aaagatcagg caatcaatgc cacactgtca ggttaccatg ccgcgtttat cgtagcgatc
     4261 atctttggga ttcttggctt tattattgcc tttttattga agaaaaaaga acctgttcta
     4321 atgaaagcgg gggataatgc atgattattg gagttttgat tgtcagcttg cttgcttttg
     4381 ctttaacgaa cgtgttcgct aaaaaaacat ggcaaacctt tttatctttg attttcggac
     4441 taatcttcgt cgcttcacta agtttgatcg tcgctaattt gtccaatcac tttggaatgg
     4501 aaaaagtaac ggaaacaaaa acagaaaaaa ttgtttccag tgctgattcc caaggagcag
     4561 atatgctttt gtataaagct ttagggaatg gaaaagaaaa agtgtatctt taccgaacaa
     4621 atgaaaaaca agaaaaacca aaagcaactg gtacggataa cgaaacaaat aaagtagaaa
     4681 agacagacgg cgatgctgaa aaagtgacga aaactactta ttgggaatac aaaaacgaca
     4741 tgtataaatt ctggttcaat attgctgata acaatcacga atatgacagc cgagtgaata
     4801 cattcaaaat cccagagaca tgggtagaat taagtacgga ccaagcagcc aagttagctg
     4861 aattagtgaa gaaacagcaa agtacgatgg aaagtgaagc aaaagcttat gttcaagacg
     4921 gtatggtaaa agcaatgact gaaaatccaa aaatgagcaa agcagagcaa gaacaacgta
     4981 cgaaagcctt agctgctgaa ttccaacaac aagcattcgc aaaattagtg aaagaagcaa
     5041 aaggcgaata atattttgaa caccatgtag gaggttgtga aaaagctgct ctgcatcaag
     5101 cagtaagaac aatcagataa aaatagcact ccatattttt catctgattg ggcttagtgc
     5161 cgtagatgca agcttttgaa caccgtttaa ggaagaggtt gtgagcctgc cgtttaactc
     5221 tgagtcacaa ggagcaattt caaaaaacag cttctcatgt ttgttgacta ttgtgacttg
     5281 tgaccgagga gttggcagga gaacaccgtt tattcgaaaa aaggaactgc gacaagactt
     5341 ttgtcacagt tccttctttt ttttgctatg ctatactaga tgtaaaaaat aggagaaata
     5401 aaatgaggga aatttattct tttgaatcaa aaatctatgc ttccgaagtc ggcaagggcg
     5461 gggcatatat tatttttccg tatgatataa gaaaagaatt cggtgctgga agagttaaag
     5521 tccaagcaac ttttgacaat gtgccgtatg aaggcagtat cgtgaatatg ggcgtgaaaa
     5581 atccagatgg ttcagtctgc tacatccttg gactacgaaa agatattcgt aaaaacatcg
     5641 gaaaagaaat cggtgatatt gtagcagtta cagtgaagca aaaataaaag aggttgtgac
     5701 aaaagacgtg tcacaacctc ttgataaacg gtgttcaaaa gccagtttct cggtagtaag
     5761 tcaaatttat gcaaaaatgt ggaaacccat gtttacaaaa tttttcttac tactcgaaac
     5821 gaagcggctt tatcacaacc tcttgataaa cggcgttcaa tcagctgtcc ctcggtatta
     5881 gctcaaaaac ggcaaaatat gagaagctat ttttccattt ttttctctca tactcaggtc
     5941 ataacgctga tttcacaacc tcttctttca taatggtttt tcaccatctt tataataaac
     6001 tggttatttg atatggaaat ctattttttc caaccaatct aaaatcttgt aacctaactc
     6061 ttctgacatc ggaaaagagg cgttgatttg tgcattggag atatggtcaa catggatacc
     6121 ggaagtgatc gtacaacttc ccggaacggc agttttgatg atcacagcta ttttttcggc
     6181 taagacatcg tctttatgga accgaccatc gtggctaggg aaacgtacag tttgcatctc
     6241 tgtatcacga gaaaatgtag tgactgttcc gatatgtggt gtatctccgc ctgttaaagt
     6301 gatcaataag tctttaccga ttactttaac agcagcttgg atcgtgaaac ctagtttggt
     6361 tacttcaact atcgtttctt tttccatatt aatcaccctg ccaacgtttt gaatgctcat
     6421 ttacaatttc aaattgatcc aaaagtttca ttgtatggtg attaacaaga tcttggatcg
     6481 tttgaggctg attataaaaa gcaggaattg gtggtatgat ttgcacacct agtcgtgcta
     6541 atttagtcaa gttttccaag tgaatggcat ttaatggcgt ttcacgagga acaatgacta
     6601 actttcgttg ctccttcatt gttacatcag cagcacgtgc aatcaggttg tcgcctaaac
     6661 caacagagat acctgcgact gttttcatac ttgccggtac gatgatcatt ccatcggtca
     6721 aaaatgatcc gctggcgaca gcagcaccta aatctttgtt tgaataatgg taatcaaaca
     6781 ggctttcgaa ttcagttagt gataagtcgg attccagctt taagttctgt tttgcccatg
     6841 gactgatgat tccgtgtgtt tctatcatcg gatattcttt tagtttcttc actaggttga
     6901 tggcataaat ggtgcctgaa gcgccactta caccaatcac gatttttttc ttccgttgtg
     6961 ccaattgttc cattttccgc actccttttt atgatgtcaa aacagttgca gatcattcat
     7021 tatttcaaat atttttccca atcttctact tcttggaatt ttgcacgtac aaaatgttct
     7081 ttcatatcga aaggaactgt accgtcaaag atagtttttg cacttatccc acggaaacga
     7141 atagagccag gattatattc tggtaattct gatgggtcca atggatggtt cctcattcct
     7201 gggatcacga tgatatcttt atctccttgg aaacgagtgt tgaaagtcca aactacatcg
     7261 ttcatgtcga aaatatcaac atcttcatcg actaaaatga ctgtcttcaa ttctttgaag
     7321 gctgataaag caagaatagc tgcttgacgc tgaatacctt catccgcttc gttttcttta
     7381 cggatttgca tgatggtcat caatttacca ccacctgcag gaggattgta gacatttact
     7441 acttttcctg gaatagcttt atcgaccaag gcaagaatac ttgcttctgt aggaataccg
     7501 gccatagata cgtgttcttc actgggacca atcgttgttt gcatgattgg attgtctttt
     7561 cggtgagtaa ctgctttgac tttcactact tgtacagctg gattagcatc cccagtatag
     7621 ccaggaaatt ctggcattgc ttttcctgag ttcgtgttga tatcttcttg cattgtttca
     7681 tttggcatga tttctgcttc aatgatgaat tcagcacgag caatacccac ttcatcgact
     7741 gctataccat caacgatttg aataggttcg ttacgcaaag ctcctgcaca ccatagttca
     7801 ttgtagccaa gtggagtggt aggtggttcg aaagtcgtgc caatggcaat tgctggatct
     7861 aaaccaatat tgatagtgat aggcattggt ttattcaagg cttcgaattg tttttggaag
     7921 tgaccaatgt ggcgtccgcc aggcatgatg tacataccga tcgtatcttt atcttctaga
     7981 accatacgat ggatcgttac atcgctcatt gttttttctg gatctgatcc taagactaca
     8041 cccattgtga tgtatggacc agcatcttca ggagtatttg taggtgcagg caaaatgttg
     8101 cgaatatcga agtctgcatc ggtagctaga tgaacgactt cttgtgctgg agcgtctgct
     8161 ttgtctactt tcacaggttt gattggattt tctacagcgt ctttcaagaa acgaccaagt
     8221 gttcgataat catggtgcaa aatttcacct acacgtttac gactagccat tgttccgatg
     8281 ttcacacgtg tgccggggaa acctttgata ttgttgaaag tcatagctgg tccttcttga
     8341 gtcggacgtt ttactgtacc gccagcaccg atataacggt aaacaccaga taattctgct
     8401 tcaggatcga tttcaatatc tgtttcgtga tattgacctg gaagtgtttt tagttcttcg
     8461 atgacttttc ttaaatcata cggttgatct gtcatgagag attcctcctt agttgtcttt
     8521 acctatctat agtaaaaggt gaaaaagagg aaaacaattc aacttgttcg gtaaagtatt
     8581 ctacaatgga atggatgcgt ttttttgata atagaataaa ctatgatata cttatcacta
     8641 aaagaaagcg catacttagt gttggaggaa ggaaagtgaa aaaaatggag gagtcatttc
     8701 ctaaagcagt aaaagtggaa aatatagcga atattttgaa agttacattt gaaaacggag
     8761 aagtaaaata cgtgaaatct cattggacag aagagataac cgatgccttg caatttggaa
     8821 aaaaaggaag aggaaaaaga aaaaatcttc tcgcactttc tcgtaatatg tggattggga
     8881 ctgaagtaac gattgaagca gatggaacag tattcataaa tggaaaagac aggtatacgc
     8941 cagaagagtt gtggtacaaa ggaaagaaaa gtataccaga attataaaaa ataattaaaa
     9001 aaaacagcgc gaaacaagga acatattact tcctcgtttc ttgctgtttt ctactatttc
     9061 agcgctctta tgactcaaat ctaaatgtgg ttgtcacaac cttattctgc acgcaaagcg
     9121 atagcggcgt cttttttcgc tgccattcgt gcaggaagat gaccgccaag catcgtcaat
     9181 atagtagaga cgatcactag aataatcgca tggacaggat ttagttgagc aacatttttt
     9241 agatccgtca tgttgtataa gatgctattg atagggaacg tagctagcca tgcaataaac
     9301 acacctaata tcccagaaga aatacctaaa atacacgttt ctgcgtcaaa tacgcgagtg
     9361 atgtcttttt tacgtgcacc taaagctttt agtacaccga tttcttttgt ccgttcaatc
     9421 acagatgtat aagtaatgat actgatcatg atcatacttg ttactaaaga gatccctgca
     9481 aaagcaataa ggacataagt gatagcatcc atcaatccgc cagtcagttc agtcatcgtt
     9541 ccagctaaat cagtataaat gattttatct tcatctgatt tgcctttatt gtaatcatct
     9601 agataatcca agatcttttc tttgtcttca aaattgttcg gataaatcat gatacttgaa
     9661 ggcaagctgt ctccacctaa ataactaagc aatgtttgtt tggcattttc gtcaactttt
     9721 tcagtagtta agacatttac accactgtct ctttgagctt tgacgatttc tgattctttg
     9781 ttttcgttga cgatttgagt cgttaattga tcgctataag cgattcctgg agataagaga
     9841 ttcattgtag aagaagattt cacgcgtagg atccccgaga tcttcaattc atcagaagca
     9901 ttctgataca tcgcatcata atctgtatta gggataaaat tcccagttgg aagttttgta
     9961 tagtaagtat tgttgtttac taatttgaat gttgtaccaa cgatatcatc aaaatcaagt
    10021 ttttcgtctt ctttcacatc aaatccaaga ttcttcaatg cgttgatatt agtattatta
    10081 ttgccgtcta cgatcagcac gacatctgtt gctgaggctg gataacttcc ggcaagtaaa
    10141 gaataattgt cttttaaaaa gttttctttg ctagtgtcaa gctgtgtagg gaaacttgac
    10201 actcccacac ctgtcattgc tgacatcgta ctagataaag aaagagattc cgtatcagga
    10261 ttttgattcg agaagctgac aggctgtact ttgccattta catcacgtaa aagattgatt
    10321 cctgtagtac gggtaaatcc gatattatta cttaagttag gatcgatgtc tgttacgtaa
    10381 tcgacgtatg tttgatcaat cttattcgtg tgctgtgctt tatcttcttc gctgactttt
    10441 gcagtaatcg ttttgctgtc tggataatcc gctttgcttg cacccaatcc ggcatcgtca
    10501 cgagtctggt tagtcgttac cttagaaata gtaatcggaa atttcgccat cgtttcagct
    10561 tgcgtgttat cgatttgttt ttggaatcca gaagaaaggg acagaacgat cgcgattccg
    10621 atgatcccaa tactggaagc aaaggaggtc aagaaagtac gtcctttttt tgttcggata
    10681 ttgttgaagg aaagctttaa cgctgtccaa aaactcatct ttgttctgcg taagttaaat
    10741 tgatcatctt ttggtcgttc gatgtgagga tgggaatctg ttaagatctt accatcagaa
    10801 aattcaatga ttcgatctgc gtattgatgg gccagttcag gattatgtgt gaccatgatc
    10861 actaattttt ctttcgataa ctcttggatc aattccatga tctggatact tgtttctgta
    10921 tctaacgctc cggttggttc atcgcacaaa aggatatctg ggtcattggc tagtgctcga
    10981 gcgatggcca ctcgctgcat ttgtccacca gataactgat ttggcttttt atgcatatga
    11041 tttgtcaagc cgactcggtg caatgcatct tctgcccgtt ttcgtttttc gtctttagaa
    11101 acaccactta atgtcatccc aagttcaacg ttttcaataa tgcctagatg accaatcaag
    11161 ttgtaacttt gaaaaatgaa accgatagag ttgtttcggt aggcatccca gtcactgtcc
    11221 ttaaagtctt tagtagactt tccgttgatt accatatcac cggaatcata attatctagt
    11281 ccaccaatca cattcagcat agtcgtttta ccagaaccac taggacctaa aatcgctacg
    11341 aattcttttt gtctgaaagc gacagacaca ccatccaatg ctttggtggt ggtttcgccg
    11401 actttataat atttttttat atcttttaat tgaagcataa tcacccttct cccgtattga
    11461 aatattcggt atactaataa gtatatcaga acaatgatcg tagtatacga aataaatgtt
    11521 aactgaagat gaactacaag aaaaaatcta gaaatttttc aaaaaacaga gggagatcaa
    11581 tgaatcgtat tttagtagta gaagatgatc cgcaattaca gttgctttac cgttctgtgt
    11641 tggaacgagc gggcttttca gtcatcaccg cattgaatgg agaggatgcg ctgaaaaaac
    11701 tagaagcgac acgtatagaa ttaatcatta cagatatcat gatgccgaaa atggatggct
    11761 atgacttgct agactcatta cgtagcacca gatttgacac acctgttcta atgattacgg
    11821 caaaggccga ttttgaggat aagaaacgcg ggttcaaatt aggagcagat gattatatga
    11881 caaaacccat cgatgtcaat gagatgatcc tacgtgtgga agcattgctt cgacgttcaa
    11941 agatcaatta cagcagtagc ttgataatcg gacaaacaaa attagagcta gatgcttata
    12001 cagtgttaca agatcagcat tcgttgattc tgcctcaaaa agaatttctg cttctgtata
    12061 aattactgtc ttatcccaac aagatcttca ctcgccaaca gttgatggat gatatttggg
    12121 gattagatac gaatacagaa gaacgaacga tcgatgtaca tatcaaacgt ttaaggaccc
    12181 gttttgaaaa gacggaagat tttcaaatca tcacagtacg gggactaggc tataaggcgg
    12241 ttatttaaag atgaaaaaaa agctattttc acaattatgg atctattttg ctagtattgt
    12301 cttcatttcg atcttagtga cactgctgtg ttttctcggt tttattttct tattgtctcg
    12361 acattctatc tcgcctgctg aatcggctgg aaaaccaacg ttatacccgt tattcgtctt
    12421 tgcggggttc agtatggtcg tgggtacagg aatatcgatt tttgttgggc ggcgtatcct
    12481 gcatccaatc agtgcgctag ggacaaatat gagtctggta gcaacgggag atttttcgat
    12541 tcgaatggat gaacagcaga aagtagcaga agtacagcaa ctgtataaag atttcaacgt
    12601 tatggtccaa gaactcaata gtatcgagac actgaggaat gactttgtgt ccagtgtatc
    12661 ccatgaattc aaaacaccac tagcaacaat ccaaggatat gttcaattgt tacaagcccc
    12721 aaatctctct gacgaagaac gccaaatatt tttatatcgg attatcgaaa gtatcacgca
    12781 gctttcacaa ttaacagaga acatcttaaa attgaacaaa ctggaaaacc agcgtatcca
    12841 actggaaaag aaagagtatc gattagatga acaaatcaga gaagtaatcg ttttttttca
    12901 gcctaaatgg gagaaagaac agctagaatt ggatatcgaa cttgctgcag taaattacac
    12961 aggcaatgaa gagtttttgt accaagtgtg gctgaatatc atggataacg ccataaagta
    13021 caatcaaatc aatggacaga ttcatatcaa actatttgag acagccacag agattgtact
    13081 ggaagtgacc gattcaggtg taggaatgaa tgaagagacc agagatcgaa tgtttgaaaa
    13141 attttatcaa ggagatacaa gtcgtcaaat ttctgggaat gggctaggac tttcactggt
    13201 taaaaaaatt ctagaactgc atgatggacg tattgattat agcagtatcg aaggagtagg
    13261 aacgacagca atgatacggt taagtaaaca ataggaaaaa gaataaaacc cctaaaaatt
    13321 ctattgaagg tttttagggg tttttagtcg tttttttgtg attagggaaa tgttcttacg
    13381 gttcttttgg cataaaaatc gctgctttct tattgaacta cgagaaatgt tagaggtgtc
    13441 attgaaacaa ggacatgatg caagaaagag agcgaaagtc atgcgtcgct ctcttttttt
    13501 gggtggatag atggacagaa aatgtagggc ttagttttct tcttgattta aatactccca
    13561 aaatgtatct gaagccaaag aatgctcacc gactttacga gtaaatgcaa gataactctc
    13621 tgttgttgga gaaataggga gaatcacagt attttctttt aatagattct tgactgtttt
    13681 ttccataaca atagcaatcc ccatgtcatt tgcaatcata ttgatgatca agtcgattct
    13741 tgctccttta taagagattt ttggttcaaa tccagcttcg cgacacagat ctagtacagg
    13801 ttgatacaag ttcgtagtgg ggccaaggag caaaaagttc tcctttttca gttcgcttag
    13861 atccaatttc tttttactag ctaaaggatg atttttaggt agaactgcta caaaatcgtc
    13921 tgtttcagta ggcaaccact caatcgaact tggtggggcc tggaaacttc tagcaaagtg
    13981 gatcgtgtca ctatgttcat tggcgtcatt gtaagttaat tcgacacttt ccacttcttt
    14041 caactggact gtaaattcag gatggcgttt caaaaactcg gtgattttgg aaaaaccttt
    14101 ataattgatc attgtcggta tcgttaagat atttaatgaa agattgttgt ctttcgtata
    14161 ggtttcaagc acttcttgca tagcatggta ttgttctacg acatttttta catatgggag
    14221 caataagctt ccagcttcgg tcaattcgat ttttctatgg gcgcgttcga ataatttgac
    14281 gtctaattct gtttctaacg aaagaatctg tttagaaata tttccttggg tggtgaattg
    14341 tcgatcagca gttttggtga aattcattgt ttcggctaaa tcaagaaaaa cttcgaattt
    14401 atggatatcc atcatattcc tcctacttat tccaataaag aatactttaa cacaaaaaat
    14461 gaattggtgt aatcttctct gtctgttaca atctttcttg cagcgaaagc aataattaca
    14521 gaaatttttc tgattttttt cgcttcgtag aaaaagagag gagttttttg atgccaactg
    14581 gagttttgat caatgtaggt tccgttttgt ttggcggact aattggcgga ttagtaggaa
    14641 ataaattaag tgaacatttt aaagcacagc tgacgatggt ttttggtgtg tgttcgatgg
    14701 ggatgggaat ctattccatt gcgccgatga aaaatatgcc ggctgttatt tttgctttag
    14761 tgataggaac agcgattggt ttgattgttc atttaggtaa tgggataaat aagggggctg
    14821 cgctgatgca agtacctatt tctaaaatct tccctagtga aaagcttggt atgacccacg
    14881 atgaatttat cagcacatta gtgacggtca ttgttctatt ttgtgcaagt ggtacgggga
    14941 tctatggaag tttagattct gggatgacag gagacagtac gattttgatt tctaaatctg
    15001 tccttgattt tttcacagca gctatttttg cgtgtaattt agggtatgtc gtttctgtga
    15061 tagcaattcc ccaattcatt attttttata ttttgttctt attggcaaaa tttatttatc
    15121 cattgactac accagacatg atcttagatt ttaaagcttg cggcggattc ttgatggtcg
    15181 ctaccggttt tagaatgatc aacgtgaaaa tgttcccagt agctgacatg atacctgcaa
    15241 tgatcgttat catgccactg agctggatgt ggacaaattg gattatgcca ctgttataat
    15301 aaaaatcaaa gagaagccat tttttgctga ctaaaaagga tcaaatcttt tagtcagttt
    15361 ttttgtattt gacaaactga ttttgaaact atattatatg ttgtataata tagattgaaa
    15421 taaaatatta cacttgagaa tagaaggtga cgtaatgcac atccagattc caactgttct
    15481 tcttgatggt actgtgctag cagcattaag caaggaagat atgtatgggt atgccttaac
    15541 aaaaatcgta caagaaagtc tctctgtcag tgaatctaca ctatatccgg ttttgagaag
    15601 attgaaaaaa gaaggatatc ttgagaccta tgatgaacct tttgaaggca gaaaccgtcg
    15661 atattactcg ttgacaccaa tgggaagtcg tcatttagaa gaaattattg atgaatggat
    15721 tgtttttcga gattctttag accggttatt ggaggagagc acaaatgaat gaatatttga
    15781 atgaagttgt ggatcatctt caagaaattc ctgaagacga tcgttttgat ttgatccagt
    15841 attatgagga atatttttta gattctggaa aaacattaga tcagatcatt gatgaatatg
    15901 gtacaccaaa gcagtttgcc ttaaaattaa aaatcagtta cttttcagaa gaagaacaag
    15961 cagaaaacga gttagtgaaa aatccaaaaa gacaaattcg cctgatttgg ttaatcatta
    16021 tcggtttatt tgcttctcct gtcctcattc cactagcact ggggtttata ttggttattg
    16081 ggggactatt ggttgcttta ctagcgctca tttttagtat ctatgtttta tgtatcagta
    16141 tcttaggtgt tggcctattc atgatcatct caggaatcag tgtgtttgga caatcgattc
    16201 tgagtggatt attctttatc ggtgtcggtc tgttcgccac aggaagcgca gtatttattt
    16261 cgccactgtt gctgaaagga acaaaagggc ttttttatgt catgatgaac tttgttaaat
    16321 ggatgggacg acgttttatt acaaaaagag atctgcattc agcgggcgtt tgaaggagga
    16381 cacataaatt gagattgaaa cattttttga ttaccggtat tggtctgatg cttactggtg
    16441 gcctcctagc aggtgcaact tatacattag gcgctcaaaa gagtctgact tgggataatg
    16501 gtcctcggtt gatcgatatg gttactgaga caaaaaaaat caaggatgca aacaaaatcg
    16561 tcatccatgc agaaaatcaa acagtttcgg tcatacgcgg attggatttt gagataaaaa
    16621 cgacttatga taagacagaa aagccagaaa taaaggagaa aaatggcgtg ttgactgtga
    16681 atgcagtcaa aaaatcaaca ggtgcaatca ttagtttaga agacacaagt gcacaaatca
    16741 ctattgttgt ccctgcagat catgaattat ctgaattgac aatcgaaggg aataatagtg
    16801 caattgatat caacgaagta tcaatcaaaa aagtgaatac taagacacat gatggctggg
    16861 tatcgttcaa tgaatttaca ggaggaacaa taactgcgaa agatgaatta ggctcacttc
    16921 gattagatga ggccaatgtg gataacttga tcgtcacagg tgaaaacagc aacttccatc
    16981 tagaagacag tttgatcagc aaagaaggaa aagtcatcct aaacggtggc aatttatctt
    17041 tgcatgagat ggaaaacagt ggctatacta tccaaacgtc tggtgaatct tatattgaaa
    17101 aagactacca gccaataaca aatactttgt tagaacaagg acagaaaaag ctaaagatca
    17161 ctgctaaaga cacatcgatc aatattacga cggatcaaga ggacaatgaa acagattgat
    17221 cctgtactca actggatatt ttaagaaaaa acagcaaaga tccttccaat ctttgctgtt
    17281 tttcatcgct taacgcacat aactatagta aaataagaaa gaaaatatct aaaaagaaaa
    17341 cagggtgaaa gagtatatga aggtagaaga aggtcaaaga gaaaagttaa aaacagaaat
    17401 ggaaagatta catacttata tcactcaact aagccaaacc ttctatgatc cagacaaaga
    17461 aaaagtgatg gttaattacc cgaacaattc agaaggtcgc caattagagc aagtgtacca
    17521 tgaagttttc aaacatttat tgacggtgaa gaaagagctg gattattatt ctttaccgat
    17581 tatcgataca ggaatattga agtatgatgg gaagaaggaa cgatttatct ttaaatctgt
    17641 tcgggaagat ttgcctcttt cagcagggat ggatctagag gtcttggtag aagactattt
    17701 tacagaagaa aagcactggg ttcgaacaaa attggattat ttaccgcagg cagccggagg
    17761 gacacaggca agcggttggt acatcactga agacaaagag ttagaactag aaggtgtaat
    17821 ggcgcgtatt cgaaaaaaaa attaaaaaag taacattaga tatttattga aggataaagg
    17881 agcgcaggaa gtagcatgag agaaaaaaga gcattttata tcaatggtta tgcagggatt
    17941 gtcggattgg tcgtattagc tttagttgga ttgtttttct tctatttagg tatgtggcaa
    18001 gcgaaagttt gggcactttt tttaagtatt tttctttggt tgatcacgtt attgttgtta
    18061 agttcggcaa ctgtagtcag tcctaatcaa gcaaaagtaa ttttgttttt tggacaatat
    18121 ttaggtacga ttcgagaaaa tggctttttc cttacgatac ctttggcaca aaaaatgact
    18181 gtttcgttga aagttcggaa tttcaatagt tctgttttaa aagtcaatga tttggatgga
    18241 aaccccatcg aaatctcagc agtagtagtc tttaaagtcg ttgatacagc aaaggcatta
    18301 ttcgatgtag cgtattatca agattttgta gaaatccaaa gtgagacagc cattcggcat
    18361 atcgctagcc aatacccgta tgatacattc aatgagaatg atttgacgct tcgaggaaac
    18421 acgacggcag tgtctgacga gttgcaaaaa gagctgcaag agcgtttagc agtagcaggt
    18481 gtcgaagtaa tcgaaacacg cttgaaccat ttagcctatg caacagaaat cgctagtgcc
    18541 atgctccaac gtcaacaagc aaaagccatt ttgtctgctc gacaaaccat tgttgaagga
    18601 gctgtcacaa tcactcaaat ggccttagag caaatcgaag atggacaaga aatcaatttc
    18661 accgatgatc ggaaagtcca attgatcaat aatttacttg tctcaatcat tacagacaaa
    18721 ggaacgcaac ctgttatcaa tacaggcgac atgaaagacc gtaagtgaca tactttttgt
    18781 aagataggaa gaaggagttt aggacggatt attgtcttga actctttttt tagttgtttt
    18841 aaagaaaatg acaaaattga aagaagaatg atagttgatt cgaaggcaga attgttcgct
    18901 tttttctata atcagtatat aaagtaagag agaaggggaa aaagtgaaaa aacttctgat
    18961 cggtctactt gttttcatcg ttgtggtact tgcaggggtg aaaatcgcag attacgtagt
    19021 tatgggtgga gaagaatact atgtacaaat tacgacagat ggtacaaaaa cagtttcaaa
    19081 atcagactct ggacaagaaa cagtcaacta tagatatatc ctgcctggct atacgaaaga
    19141 gggtaaggaa aaagaactgg acttcaaggg acaattgact cgaccgcttc gtaaaggagc
    19201 atatttaaaa atcacctgga acaaaaataa aggtgtcact tcgtatgaag aagtcgaaaa
    19261 aaaagacgtg ccgaaaacag cactggaaaa actaattaaa gggtagggaa agaaatgaac
    19321 aaaatagtcg aagtacagaa tgtaacaaaa gtatacggaa aaaacaatga gaaaaagacg
    19381 caagcattaa gcggcatcag cttcgatgta gaaaaaggcg aatttattgg gatcatgggc
    19441 gcttctggtt ctggtaagtc gacacttttg aatattttat ccacattaga taaaccaaca
    19501 gatgggcata tccgaatcaa tcaaaaagat gtgaccacat taaaaggaaa tcaactagca
    19561 gattttcgtg cgaatgagat tggctttatt ttccaagatt tcaatttatt agaaagcttg
    19621 acagcacaag aaaatatcgc tgttcctctt tctttacaag gcgtcagacc aaaagaaatc
    19681 aagcaacgtg tccaaaaaat tgcagaacgt ttatccatct ctcatatttt ggaaagctat
    19741 ccttctgaga tttccggtgg acaaaaacaa cgggtcgccg ccgcacgtgc attgatcaca
    19801 caaccaacga ttctattagg ggatgagcct acaggtgcgc tggattccaa aagtgcccgg
    19861 gatcttttag atacgatgga cgaattgaat acaaaagatc atgtatcgat tcttcttgtg
    19921 acgcatgatc cgttttctgc aagttattgc caacgtattt tattcatcaa ggatggcggt
    19981 atccatcaag aagtgaaacg tggagatcaa agccgtgatt ccttctatcg agaaatacta
    20041 tctattcttg gaaacttgga acagtagggg gagattgact atgctttgga aattatcact
    20101 tactgggatc aagagccgtt taagagacta cttggtgtta ttctcaggat tagttatggc
    20161 atctgctatt ttttacatgt ttgaatcaat ggcaagcaac gaagcttttt tgaaaagtaa
    20221 tacgatcgtt gcggctacag ttatcatttt ccgattcggt tctgttttac tagggatcat
    20281 cacattcgtt tatattttat atgcaaattc ctttttgatg acgatgcggc aaaaagacta
    20341 tgcaatgttt atgatgctgg gagcaaaagg cagaaaaatc gcacagatga ttttttctga
    20401 aacattctta gtagggatcg cagctacttt agtgggttca gctgtcggtg taggattgac
    20461 aagtatcgtc caccgattat tagtggatca gttagatctc aatatttcgc attttacacc
    20521 ttttagtatt cacgggttgt tgatcactgt tttgtttttt gctgttttgt ttttattagc
    20581 tgctgttgtc aatgcttttt cgattgtcaa aaaaccgatt ttagcattga ttcgtgcgga
    20641 tcagacacca acaagaatca agcagcataa attcttgttt ttccttgaag tagtactggg
    20701 aattgctttt ctaggctgcg gttactatat gatgaaacac gtggggacct atcaagtctt
    20761 tggtataggg gtagccttag ttacgatcgt attaggaact tatttcattt tccattctgt
    20821 gatcattttc tttttatcat tactaaagaa atcagaacag atttcattga aaaaattgaa
    20881 caatttcaca ttgtcacagt taaactttcg tattcgtgaa tatacacaaa tgctctcaat
    20941 ggtcgctatg ctgtttgcct tggcactggg cgctttgacg gtaggattag gttttaaaaa
    21001 tgaaattgca gacttgacag aaaacaattc tggatatgat ctagtgatga ataatcctcg
    21061 ggcagaagat cagcaaaaaa tcaaagaact ttcaccaaca ctttctgctt cttatactca
    21121 aaaagaagat gcgggaacga tttactataa tgcaagtgaa tttgcggctc aaccattgat
    21181 catgataaaa catgaagatg gaacaccgcc gaagatcacg aaagaaaaag ttcctgctaa
    21241 aaaaatgaat gaactgactg tacaagagga acttcgaagc tatgaattgc cggaacagca
    21301 agaaaaagaa atcaaggtag tcagtcaatc agaatttgat caactgaact tgccacaatc
    21361 cactttacag ctagttcagg taaaagactt ccaagcaaac ctgaaaccaa tcgaaacact
    21421 tgttgctcaa aataaaatga acaatccatc tttgcaaaaa gtagagtaca gcaaccagaa
    21481 atatgaaatg tatgaagtgt ataatggtct attctctggt tttgaattta tgggattctt
    21541 cctaggttta gctttcttga ccatgcttgc tagctgtttg atgttcaaga tcttgtcagg
    21601 aagcaagagt gacattgctc gttatgaaat gcttgaaaag ataggaacac gacgcgggtt
    21661 gcttaaacaa tcgatccgta gagaagtagg cgtgttattc ttagcaccag gaatcttagg
    21721 tgtagtccat gtcttattcg gattacaaat gtttggtcta ttcatgcaaa atccatacaa
    21781 agatatttgg gttccgttta cgatattctt cgtattgtat ttcatctact atgtactgac
    21841 gacatggtta tatactggaa tcgtattgaa aaagagattg tgaaaaagct gtcctgcatc
    21901 aagtaataag aacagccaag caaaaatagc tcctcatatt tttcgtttgg ttgggcttat
    21961 taccgcagat gcaagctttt gaacaccgtt tgttcggaaa aagaggttgt gacaatattt
    22021 ttgtcacaac ctttttttca cggtctttta ttagaatata gtaaattttt tctcgttact
    22081 cacggaataa gctgttaaga aaacggtttc catgtatact ggaatcagtg caaatgaaag
    22141 aaaagagggt gcaaggaaca tggaaatcaa tgaacaaaat tttggcgaaa actatcactt
    22201 gattacgatc acgaatcaaa atgggacatc tttgtctgtc agtgatcttg gggcacgggt
    22261 tgttcatttt caaacaataa tcgataatga aaaacgagag ttgatcttag gttttgatga
    22321 agcaaaagaa tatctagaaa aagaccctta tatcggtgct tcaatcggac gtatggctgg
    22381 acggattacg aatggcacat tcaaattaga tgatgaaacg ttcgaagtaa aaacagaccc
    22441 ggacactggt cattgtttac atggtggtgc acctagcttt gaagccaagc agtggcatta
    22501 tacagtcatc aatggtgaaa acgaagcatc ggtcattttc tatcagacta gcccagatat
    22561 ggaaaacgga tttcctggaa ctttagatgt agaagtacgg tatacgctga cgaacgatga
    22621 tacatggcga gtgacggtcc aagggatcag tgaccgcaaa acagtattca atcctacaaa
    22681 tcatgtctat ttcaatttaa ccggtgatcc agcacagtca atcgacgaac atatactttg
    22741 gttgaatagc cgattatttg ctacgctcaa taaagataca acgcctacag gagaagtagt
    22801 ttcagtaagt ggcacagcat ttgacttcca acgtcctaaa aaattaggtg aggtctttgc
    22861 cagtgatttt cgccaatcaa tgatagtggg cggcatcgat catccatttt tgttgaaaaa
    22921 acaatctggt ttactaaatg ctgcggaatt gacaagccca gatgaaaaag tatcgatcca
    22981 agtaaaaaca gatgcttcag gaatcgtgat ttttacagca aattttggag aagatggacc
    23041 ggaaatgcgc gggaataaat tagtaaatca tggcggtatc acatttgaga ctcaagaact
    23101 gccaggagcg gagcgacttg aaagttttgg agatgtcact ctggaaccta accaagtacg
    23161 cgagtttgta acggaataca aaataaatat aaagaaatag aataaattaa aaagggagtg
    23221 tgacagtgtc acatcccctt tttaatttat tctatttctt taaaggcaga tcaagccaga
    23281 atgatttgtc taaacttaac aaggttagta caggaacagt tgcagcttta actgtacgag
    23341 tagttgtttc tggtatctgt cttgccgtaa aattgttcca caaatcccag attgaagcga
    23401 cacggaagat ggaagaagcg ttgttgacgc gttttatcgt tgaaaattct aatcctgtga
    23461 ttggcacgcc atctgttgtc catttgattg gcacatcgaa tcgcttagac atattgagga
    23521 tttcagcgtt tgcttcttca gttgttgctg cttctttttt cggttcatca tattttgtca
    23581 ggtcgtacgc ttcgatgatc gcgttcaatt tttgtgaata ttggcgatct gtagcataac
    23641 gtccttccaa atatttggtt gcttctttgt agtcttttgt ttccgacttc caagttggtt
    23701 tgtagaaatc aggattgcca gtcagtccat ttttgatcaa gtcggcatag tcttccaaac
    23761 tttccttgta agaaggatat ttacggaatt gtgcgcggat ttggtagctt tgtccggaac
    23821 tatcttgttc aagtgtattg aaggtcacgc tgcttccttt atatgatcct ttgataccaa
    23881 aaaggttatg atttggttcg cttgataata agctgtttcc agatccactt tcaaggatag
    23941 cttgagccat catgactgat gcgtagattc cttctttttg cccgatttcc tgtgcatcat
    24001 ctgcaatctt tttcaagaac tgatcagttg tttggttttg actgaaatgg aacgagccat
    24061 ctaatccatc taaaatagat gagccgcctg tcagcgaacc tgaagcagca ggtaaacctg
    24121 ctaagtttac tcggacagca aaatcaggcg tgaaatctga aatagaagtt actcgaactt
    24181 tatctcctgg ttgaggcgca tggataaatt gtccgttccc aatatagata cctacatggt
    24241 gagtaacacc ggaagaaggt ccccagaaga cgaggtcgcc aggttgggcc tgacttactg
    24301 gaatctgcgt acctgcatat tgctgttcgc ctgtccagcc accgatataa tgaccggttg
    24361 ctttcatata tacgtaataa gtcaaaccag aacagtcaaa agtatttgga ccttttgctc
    24421 cccatacata tggttttcca agatgcttga atgcttctcg aacgattgct tgttggacat
    24481 tcgttgtatt tgtaggcaat acagcatcaa ttggtgattt tacaacaaaa tcagaagaag
    24541 cagaagatgg attctctata ccgctttgat ttgctgtatt tggacgagat gaagcattgt
    24601 tcgttccgcc attcgatgaa ccattgtttg gatggtttgg ttctctctga gtatttccat
    24661 tgttattcga tccagatggg tttgtttgat tcgaagaatt cccgctgttc gaattattgt
    24721 tattattgtt tgaattattt gaaccattat tattgttgtt attacccaca tctggtttgt
    24781 ttggcgtttc cggatctggt gtactaggga cttcttcact aggatcagtt ggtttctctg
    24841 ttgaagtatc cggtccagct ttaaattctg cagtaataga taaattgttc gtacctactg
    24901 taaaggtctt tgcatcataa tttacttctt tggcaccatt aacagcgact gaagcaattt
    24961 gttcaccttc agctggcgta atagtgtaag ttaccttcgt ttccgctaat aaaccttcta
    25021 ccgttccact ttccgtaata tccaaaggtt gctgttcagg ggaatccgct gtcgaagcag
    25081 tcagttggat cttaccaatc aaagaaggag caattgtaag ggaatatgtc tcggctggct
    25141 tttcattttc tgatggatca gccggatcaa tttcagttgg ctcggtcgat cctgttgttg
    25201 agttagttgt tccagttggg tcttctgtaa aagaatccgt tgtttcacta gtagctgagc
    25261 tggatgtagt cgtatctgtt gtgactgttt gatcagctat ttgattatct acggcaaata
    25321 cggtattggt cgacaaaaca gacggagcaa tcagccaagt agcagctagc aaacgtatcg
    25381 tttttttatt tttcataata aactagtcct tttcctttca ttgattttct gtcttattca
    25441 gcattcatta gacatttttt ggaacagata atcgtgtcga aaagcttttt cttttcataa
    25501 atctgtctgt gtattctaaa taaaacaatt tgtaaataag caacgagcat tcattcgttt
    25561 ttctttgacg ttcattttat tgcatagacc acctttgttt ggggataaac aagtggggat
    25621 aaaaatggtt atgcatatgt aaattatacc aaagtttgga aagtatcaca aaaatttata
    25681 aaaataatga tcctaaaaag aaaaagactg aaatatgtga taaacatctc atacttcagt
    25741 ctttttcatt aactattcat aagacattag gtcttcatca aacccgctaa cgagagtagt
    25801 gatcattgct acgactagac taacagccag tcctgctaag taccagaaaa aagtaccgga
    25861 aatgaatgca ggcaatgtgg tcagactacc aaatacaaaa gcgatcgctt tcacttgggc
    25921 aaatcccatt acagctccac cagcagctgc accaatgcaa gcagcaacaa atggtttttt
    25981 caatgggatc gcaattccgt aaacagttgg ttcggtcact cccgcaagta aaatcgaagc
    26041 aaaagcagaa ccggagatcg aacgggtttt tgaacgttta cttcgcagaa atacacctaa
    26101 agctgcacca gccattccaa aattggctgc tcctgtcaac ggaacgattc ggtcgaaccc
    26161 ataagcagag atattactga tcatgacagg gtttacagcc cattggatac caaatattac
    26221 caaaatattc caaccaccag cgatgattgc gccactgatc ggtccgttaa tatccatcat
    26281 ccaagtaatt gcggcagcta aaccttcgcc aaggtaaaca cccgcaggcc cgaataccat
    26341 taacgtcaaa ggaaccgtga taactaatga aagtaaaggt acaaatacta attgcatatt
    26401 ttcgcgaata tattttttga ggaatttttc aacaaaagaa taaaagaaaa tacctaaaat
    26461 cgccggcatg acagtagacg tatactgcat cagtactaca ggaatcccca gaaaatcagt
    26521 agctgctttg ccactgtctg aaagtaaaga ggtcagattc ggttcaatca gtgctgcagc
    26581 aatgacagcc gcaatatatc cattcacttt aaacgttttg gcagcagaaa aagcaagaaa
    26641 gattggcaga aaataaaaga cggcattcga cgcagcagac aaaatatgat aagttccgct
    26701 tgtttcacta agccagccaa tcgtagtcgc taaaacaacc agacctttta atataccaga
    26761 accggcaaac agcggaatga tcggcgtaaa gatttccgat atcaaaccaa atagtttttc
    26821 gatcggattt agcttttctt tcggagctga atgatcgatt tgacgcgtca attccttgta
    26881 agcaggagcc aatccctgat ccatgattat ttctgcatat ctatcgagta aaatgaaata
    26941 cttaatggaa gggatttgct gttttaattt ttctgcattg gctactggct gttttttcaa
    27001 tttgatacga agccgagtcg cacatggaag gacagaatca atattgtctt ttcctcccaa
    27061 ctgttcaatc agcatatgaa tctgctcgga cagtaccatc tgttcaggat tcattgtcgt
    27121 ttccattaga agaccactcc actcactaat ataatattgg cgtaaggtgt catttcgcct
    27181 gtacgaataa atgcatgaca gttattcaac tcttttttca tttcttcatg tgggataaag
    27241 ctaatcggtg tatctggtaa caataatttg attttttcca atatttccgg gttcattgtc
    27301 tttatttctt ccgccaattc aatcgattgt acctcaagtt cttctaaaac gttttctaaa
    27361 acagataaaa aacttggaag accgtttgag acagccaaat ctattttttc agtaccaaag
    27421 gggacaggca tgcctgcatc cccgatactt aatttatcaa agtgccccat ttgtgcgatt
    27481 actcgtgaaa tatctgaatt gattactttt ccttttttca tgtttgtttc ttcctctcat
    27541 tgttaatttt agactgctga ttgttctaaa gcttcgcggt aagggataga aggttgcgca
    27601 ccataagact gcacagcaac agaagaggct agatttccat aaaggatcgc ttctttcaag
    27661 ttactaaggt ctggctgaag tgtgctgctt aatgcgccaa taaatgtatc acctgcggct
    27721 gttgtgtcaa cggcatccac tttaaaagca gggacgatac cgtgttcttt ttctgtatga
    27781 tagaaagcgc cggcactgcc tagtgtaatg attactgttc caatacctaa ttcatggagt
    27841 ttttcggctg ctgcgaccaa actgttatga tctgtcactc tgattcctgt aatgatttct
    27901 gtttctgttt catttggtat aatgatatct gtcttttcaa gtagttcagt aggaatcgtt
    27961 tcacgagctg gtgcaggatt caggatcgtt gtttttcctg cgtcttttgc aatagaaaaa
    28021 gctaggatcg tcgcatctaa gctactttca aactgagcaa taataaagtc actgtttgta
    28081 atcagttgct tgttttttag gacttcttgt tcatggaaag cattatttgc ccctgcatgg
    28141 atcaagatac tattttctcc tgctgcgtct accataatca ttgcttgtcc ggttgtggcg
    28201 ccttggattt cagctatccc agatgtatcg atgttttctt ttgtcagtag atctaataac
    28261 tgttgtcctt cgctgtcagc cccaacgcct ccaataaaag atgtccgtgc gcctgaacgt
    28321 ttggctgcca ctgcttggtt ggcaccttta cctccgccag cgtgaaaaat ctcatgcgca
    28381 tgcatcgttt cgcctggctt gggcatgttg gttaaacgaa gggtagtgtc catattaata
    28441 cttccaatta ctgtaaccgt gttcatttta caaacctcca actatctcat ttattttgat
    28501 gggaaaatta ttttttaaaa cgttttagta aaacgtttta atagttcgat aaaaatatag
    28561 cacaccttga aagctatttc aagggtgcag ttgaaaatct tttttctaat cgtacaggta
    28621 atcttttttc ttcccattct ttttctggga attggatccg atctaacaat aacttggcag
    28681 atgtttgtcc caattcgaag attggttgtg cgatcgttgt cagtttcggc ttgatatact
    28741 cgcacatatc tatattatca tagccaataa tgctgtagtc ttcagggatg ctttttccag
    28801 cttcctctaa tccgcgatat aatccaaaag ctaattcatc atttaaagca aacacaccag
    28861 ttgcagcaga atcaagaagt tctgctgtga tctggtagcc accttgtttt gagaactgtg
    28921 taggtaagag gatcagttgg tcgtgaggga tttggtaaac atcaagagca gatttaaacc
    28981 cctcaatccg agcgtggaca ttttccggtg gattttcagg atagacaagg gcgatcgttt
    29041 gatgtcctag actaagcaag tgcatgcccg ctaggtaacc accgcgaaaa tcgtctgttc
    29101 gtacggcatc actaaatcct tcagattttt tctgatccaa aacaatgaag ggccggcctt
    29161 gttttttcaa attttcgttg attgcatctg tagagacagc agaagtcgcg ataatgaatc
    29221 catccacacc tcgacgtgtg agctcggcta gatattcgat ttctttttga tgatcagaat
    29281 cagcattaca taatatcgtg acgaaattct gcttatacaa gatatcctca attcctcgca
    29341 tcagtgtact gaagaatgga ttcgtgatat caggaacgag tacaccaatt gttttcgttt
    29401 ccttggtaat catttgtcta gcaaaatagt caggctgata gcctaattct tctttggcag
    29461 ctaaaacttt ctgaacagtt ttcggactga atttctccgt atttccattt aatatcaagg
    29521 agacagttgt gattgaaacg ccggaaagtt tagcgacttc tttgattgtg atcttttttt
    29581 tcaccatgtt catactccta tctaaatcct gtggttattt tcccatgaaa tcaaaaagaa
    29641 gtaaatgaaa atcacgtgtg ccagtactta tttgctttgt tggatagctt cgattatttg
    29701 tttcgttgag cgtactcgtg cagttttcgg aaatatcttt tttatgaaca attgatggga
    29761 ttctttgtca cgatcactgc aagcatcttc agcaagcacg atatggtacc cgtgttgaaa
    29821 ggcatctaat gcagtagcat atacaccgtt tgttgtcgct acaccgctta aaatgatagt
    29881 ttcgattcca cgccggcgaa gttgaagatc aagagaagta ccaaaaaaag cacttggatt
    29941 gtattttgtg atagtcaaca tattatctgt atctttcaaa gaatccttca aaagtaaatc
    30001 agtgaattct ggaggtacag aagcagtcat tccacccata tcagtttgtt gactcaaata
    30061 agggaaagtg gagatgtcta cgttcactaa aactttcaag gcagcggtac gtttcaatgt
    30121 tcttactaac aaatcattgt ttgtcagaat agatgctgca gtgtgtggag agagtgtacc
    30181 gttatttacg atctcttttt gcatgtcgat cgcgacaaaa gcagtttttg ataaatcaaa
    30241 aatcatattt tcctttaaat cattcatgat gttccttctt tcaaataaca atgtttttta
    30301 tgtaagtaaa tcataaatcg tttatttgtc aactaaatta aaggattcct ttctttagtc
    30361 tagagaaatt cagtggtaaa ataaataagt gtgagaaaac gcattcgatg catagatata
    30421 gcgagaaata tcgtgaaaat ggaggaatca gagtgaagta tgtcgaagta aaaaatgaat
    30481 acaaacgata caaaatggga gaaacgacca ttacagcaaa tgacgatatt tctttttcaa
    30541 ttgaaaaagg cgaattagtg attatacttg gtccaagtgg agcaggaaag tcaacgattc
    30601 ttaatatttt aggtgggatg gatacacctg atgaaggtca gatcattatt gatgacacag
    30661 atattgcgca attttcagat aaacagctta ctgcttaccg acgaacagat gtagggttcg
    30721 ttttccaatt ctacaatctc gttcctaatc taacagcaaa agaaaatgtg gaattggcaa
    30781 cggaagtttc tcctgatgcg cttgatcctg tagaggtatt gaggcaagtg ggcctagctc
    30841 atcgattgaa taactttccg tcccaattat caggaggcga acagcagagg gtttcaattg
    30901 ctcgtgcact agctaaaaat cccaaacttc ttttatgtga tgaaccgaca ggcgcgctgg
    30961 attttgaaac gggcaagcaa gtattgaaat tattgcaaaa cgctagcaga caacacggca
    31021 ataccgtttt gatcattaca cataattctg ctttagcgcc catcgctgat agagtcattc
    31081 acattaatga tgctaaagta aggtctgttg aattaaatga tcatccttca tccattgatg
    31141 agattgtatg gtagggggat tttatgaaaa caagaacata tcttaaagca ggttttcgcg
    31201 aaattcggca atctaaagga cgattcattg caatcatcct tattgtcatg ctaggaacac
    31261 ttttatttgt aggtgtcaaa actgctggac ctgtcatgca aaaaacgatg gatcactatg
    31321 ttggtacagc aggactttct gatttgcaaa tcgtttctac tggtggcttg acacaaaaag
    31381 atattgctca agcagaaaag atcccagatg caaaagtaga aacaggaaaa cagatctatt
    31441 attccaatac tggcaaaaac gaagtgattc aaatattttc ttataacaaa aataacaagc
    31501 agaacaagct acaattaact gatggaaaac tcccggaaaa accaaaccag cttgtattgg
    31561 atgaaaaagc ggaagatgaa gggtataaaa tcggtgatat ttatcagatt gattcagatg
    31621 aattgaaaga aaaagagtat accatcacgg gctttgtccg ttctccattg ttcatcaaca
    31681 atttggagag aggatacgcg aacgtcggaa atggtagcgt cgattatttt gtctatttac
    31741 ctgaatccgt ttttaaaaca gatatttatt ctgtcttata tcttgatttt acaaacgtaa
    31801 agaatttgaa tacgtacagc aaagcttaca aagataaaat ggagcaaaat caagaaaagg
    31861 cagaaaagta tttacaagat cgacccgaag aacgcttaga agagctgaaa aaatcggcca
    31921 acgaagaatt agaaccggca cagcaaaaaa tagcggatgg caaagcacaa acaacgcaag
    31981 caagaacacg gttagaagat gctaaaaaac agttagcgca acagtcagca actatccagc
    32041 agcttcctga agcgcaacga acaatcgctg ttcaaacatt atctcaacaa gaagctcaac
    32101 ttgctgaaca agaaaggcaa ttagttgaga aagaacgtga actggcctct gcagaggaag
    32161 aattgaatcg aaatgcagaa aaaattgaaa atttaactaa accatcatac ctatacaatg
    32221 aacgttcaga aaatgtcggt tttcaagaat ttggtgatct gtctgatcgt atcgctgcga
    32281 ttgccaatgt ttttcctgtt ttctttttct ttattgccgc attgatcacc tttacgacaa
    32341 tgacacgtat ggtagaagaa aaccgcaggg aaatcgggac attaaaggcg ttaggttata
    32401 cgaaagttga aatcgcaaaa aaatatgcaa tctatgcaag tctagcctca gggataggta
    32461 ttgttttagg aacgatatta ggaacgaatc ttttgccaag gatcattttt gagctgtcta
    32521 acgagcggta tgatattggg agtgcgctta ttttttatga ctggccgccg attttacaag
    32581 cagctgccgc attcttcatt gccgcatttg gcgctgcaat gatcgtttta ttcaaagatt
    32641 taagagaacg gcctgctgca ttgcttcaac cgaaagcacc gaagccaggg aaacgcattt
    32701 ttcttgaata catcactccg ctgtggtcac gccttagctt taatcaaaaa atcagttacc
    32761 gaaatctctt ccgatataag tcaagaatgc ttatggcgat tatcggaatt gctggatgtg
    32821 ccgggctgat ggttgcaggc gtgggattga aagattctct aagttctgtt tcagccaaac
    32881 aatttggacc gatcattgac tatcaagcaa tcgttacatt ggattcatcc acggaagata
    32941 gtcgtcaaaa agcagcggat atgctgcaga agcaagaaaa agtaaatgag atcctagcta
    33001 ttcatactca aaccgtagaa ttgagaaaaa aaggacatgc ttcgcaaagt ctgacagtga
    33061 tcgtaccaag taaaacagct gatttggatc gttttatcca tttaactgat cttgaagaga
    33121 acccacgctc cttgcctaaa acaggaatcg ctattacgca aaaagtagca gaactatttg
    33181 acttatctac aggagatcat atttcgcttt atgatgaaga tcaaaaagaa tggaaagtcg
    33241 aagtaaaaga aatcgtgaaa aactatctag gtagctttat ttatatgtcc cctgaatatt
    33301 atgcttcagt tactgatcaa tcgatgatgg ataatgcatt tttgctgcgt acagataaga
    33361 tgacaacaaa agaagaagaa atgctttccg agaaactgct tgccacagac gctgtgacaa
    33421 atacgtcttt catgtccaaa caaatcgaaa cgcaagaaga atcaatggcg aacttagact
    33481 cggttgtctt aatctttgtc gtattatcag gactgctggc ttttatcgtc ttgtataatc
    33541 taactaatat caatatttcc gagcgtgtac gcgaattatc tacaataaaa gtccttggat
    33601 tttttgataa agaagtcacg atgtacattg ttcgagaaaa tatcatcttt actcttttag
    33661 gaatcatcgg aggatttggt gtaggatata tattgactga cttcatcttg caacaagcgt
    33721 ctatggagaa tgtcatcttt ccattagtga tcacatggtg ggcttatgtc ttatcagcag
    33781 gattaactat cgtatttacg gttattgtga tgattgttac ccatttcaaa ttaaagcata
    33841 tcgatatgat tgatgcgttg aaatcgaatg aataaaaaca gaggttgtga aatcagcggt
    33901 ttgacctgag tattagagaa acaaacggca aaatagtttt tcatattttg ccgttttttg
    33961 agctaatacc gaaggacagc tgattgaaca ccatgaatca gaggttgtaa aaaagccgtc
    34021 ctgcatcgag caaaaagaat actcaaataa aattagctcc tcatgtttta catttgagaa
    34081 gactgattgc cgtagataaa agcttttgaa caccatttat agaagaaaca agaatatttt
    34141 gctattttaa acaagaggat agacaaatac atgtctgatc tcttgttttt tattgggcaa
    34201 cagacctctt agaatgtata aagataaatt ttacaaacgc tttcggttca gttataataa
    34261 taaatgataa cttgtatata taagttgtgg taggtaataa atcagttgaa tggaggtaaa
    34321 tatccatgac atttaaagac aaagtcattt accaaatcta tcccaaatct tacaaagaca
    34381 gtaatggtga tggaattgga gatttaaaag gaatcaaaga aaaactcccg tatctgaatg
    34441 atctaggcat cgacatgatt tggctaaacc ctatttatcc aagccctcaa aaagacaacg
    34501 gttatgatat ctctgattac acagccatcg atcctatctt tggtacgatg gaggaattcg
    34561 aacagctaat agccgaagca aagaaatacc ggatagaaat catgctcgat atggtgctga
    34621 atcacgtttc gacagaacat gaatggttca aaaaagcact agcaggggac cagcattatc
    34681 aggacttctt cattttaaga gaagagccaa cggattgggt atcaaaattt ggtggaagtg
    34741 cttggtcgcc atttggagat acggggaaat attatttgca cttatatgat cggacacaag
    34801 ctgacttgaa ctggcggaac caagcagtaa gagaggaact attcaaagtt gttcgttttt
    34861 ggatggaaaa aggggtaaaa ggattccgtt tcgatgttat caatgttatc ggtaaagacg
    34921 aacaattgaa aaataataaa gaaaatgatg gcaaacctga atatacggac aaaccaatca
    34981 cccatacgta tctaaaagaa ttgaaccaag caacgtttgg gacggattcc actatcatca
    35041 cagtcggtga aatgagctct acgacgatcg aaaattgtat cttgtataca gaacctagta
    35101 gagaagaatt atcaatggtc tttaatttcc atcatttgaa agtcgattat gaaaatggga
    35161 ataaatggtc tactcctcca tttgattttg cagaattaaa gcgactattt catacatggg
    35221 gagaaaagat gagtgagggt aacggctgga atgctctatt ttttaataat catgatcagc
    35281 cacgtgcgct caatcgattt gtagctatcg atcagtatcg aaaacaagga gcagagatgc
    35341 tagcaaccat gatccatttg aaccgtggga cgccctatat ttatatggga gaagaaatcg
    35401 gcatgatcga cccagactat tcatcgattt ccgactatat tgacgtagaa agtttgaatg
    35461 cttataagtt actccttgaa caaggattca cagaaaaaga agcgtttcgt cggatcaaag
    35521 caaaatctcg cgataactct cgaacaccca tgcaatggtc agacacagaa caagcaggat
    35581 ttactacagg gaaaccttgg ttaaaagttg gaggcaagct agaagagatt aatgtagaaa
    35641 aagaactatc ttctgaagat tctattttgt cctattacaa gaaattgatt cggctgagaa
    35701 aaacatatcc aatcgttgca caaggagatt atcatgctta tgaagcaaat catccggaag
    35761 tttatggctt tcttcgccaa tttgaaggac agcagctgct tgtattaaca aatttctatg
    35821 ataaagatac aaggatcgcc atcccagaag gattcacaga agccaatatt cttatcgata
    35881 attattccga tactgttctt catcaaagtc tggagctaaa accataccag gcaattgctt
    35941 tgctgaaaaa ttaaaacaaa aacacaaaac gtcgttccta cgagctattc tcgtaagatc
    36001 gacgttttgt gttttattta aaataatctt cttcagaaaa tgaagagttt tcatactctc
    36061 ctttatcgcc ataaaattta ttgtagcgtt gtttgaattt acggaaaata aaagtaacga
    36121 gtaatttgac taacgtatag ataggaatac caaaaataac gcccacaatt cccattagat
    36181 cacccatgac aagtaaaacg attaagatcg taatcggatg gatctgtaaa cgatcaccca
    36241 ttactcgagg aatcaccaag tcaccgtgga gaagttgtac ggcaaaccag acaatgacaa
    36301 atttgacagc cataaaagta gagtcttgga atgcaatgat caaacctgga aagaaggcga
    36361 taaacggccc aatatatgga atgatacata acaacccagc agaaatagct aaaacactag
    36421 caaattctaa tccgatcagt aagaaacaaa cccagtagac tgctccaagg ataaatgaag
    36481 caatgatttg cccctttaga aatgaaccca attgttgatt agcaatcttt gtcaaatttt
    36541 taaaatctgt acgaaatgct ggtgggacaa tcgctaaaac tgactgataa aatttttgag
    36601 gatctctcaa aaggaaaaaa gcgacaattg gaccagtaaa taaagtaata aaaatagctg
    36661 taacagttga aaaaatgttg ctgatccgtt gtgcgccact ttcccaataa tttccaataa
    36721 atccagctat atcagacgtc acgtcgtcaa tagaagcgaa aaattgatta aatgaatcgg
    36781 caaaaggtgt tttctccaaa aattgtcgga gactctcttg aaaatcttga aacaaatctg
    36841 gcaaactact gatcaatgtc tttgcctgtt cttcaagaaa aggaataagc catatgcttc
    36901 caagccccaa taaaaataaa ataatcacat aaacgataat gactgacaag ttcctcgaaa
    36961 ggtgcttgct caaacgtttg atgactggtt ccaaaagata atacaagatc agtgcaaaca
    37021 ataatggggg caagatagta gcgataatga ttccgacagg cttgaatata aacgagatct
    37081 gatggaaaaa ataaatgatc agcgcaagta agataactat tcctaaaaca tagtaagagg
    37141 tttttcctcc taaaaagcgg atcaaacgat tcgttgtatc tgtttctttt ttcactcttt
    37201 ttcctccttt atctataact tcattataga caaaagcggg tataaaagga aaatgataga
    37261 ggttgattgc aaaaatatcg tcaatcattc atataaactt tctcatcaat taaaataaga
    37321 aaacgcttga cagttctttt aaccatatga taaattaaat cctgttgcta aaacgtaact
    37381 attacggttt gcgaaaagcg aatcggaaca ttcaaatgaa agaagatgag aagaggagtg
    37441 tcataaatga aacgattgat aggtatattg gccatgctgg ttttagcagg tatgttaact
    37501 gcttgcgggg caagtgggaa agccgaggac agtaaagaaa aattgagcgt gatgacgacc
    37561 ttttatccca tgtatgattt tacaaaagca atcgtcgggg atgaaggaga agtagagtta
    37621 ttgattccag cagggactga ttcccacgat tatgaacctt ctgctaaaga catggcaaag
    37681 atccaagata cagatatctt tgtttataat gatgaaaaca tggaaacatg ggtccccgca
    37741 atccaaaaaa cattacaaga aggcaatgtc catacgataa aagcgacaga aggtatgctt
    37801 ttacttccgg gaagtgaaga aggacatgat catgaccatg aacacgggga agaaggccat
    37861 acacatgagc tagacccaca tgtatggcta gctccaagtt tagcaatcaa gcaggtagca
    37921 aacatacgtg atcagctgat tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat
    37981 gcagcagcct ataccgagaa acttcaagca ctccatcagc tttatcaaga aacctttaag
    38041 caagcaaaac aaagatcttt tgtcacgcaa catgctgcat tcaactatct agcgttagaa
    38101 tatggattga atcaagtatc gattgctggt ctttcttcta gtgaagaacc gtctgcggcg
    38161 agaattgctg aactgaaaca ctttgttaaa gaacatggaa tcaactatat ttattttgaa
    38221 gaaaacgcca aagatagtat tgccagaacg ttagcaaacg aagcaggtgt atctttagaa
    38281 gtattgaatc ctttggaagg attgacgaat gaacaaatag aaaatggaga aaactatctt
    38341 tctatcatgg aagcaaacct cgaagcgttg aaaaaaacga cagagacaga aaacccgcta
    38401 gaagatactt tgacccccaa aaaagaaaag agcgtctaca acggttattt tgaagacgag
    38461 gatgtacaag atcgctctct ttcagattgg gcaggaactt ggcaaacagt agatacgtat
    38521 gtagaagatg ggacttttga tcctgttttt gaatataaag aaaaattgaa tcaggataag
    38581 acagcacaag aatataaaga atattatacg aagggctatc aaacagatat tgcttcaatc
    38641 aagatcagtg agaaagagat ggcatttacg tttaagaatg gaacaacgaa acgttcaaat
    38701 tatcgttatg tagggaaaga aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat
    38761 ctattcgaag cagaagataa ggaaagtggc gcttttagat atattcaatt cagtgatcac
    38821 acgattgcac cggctgactc cgaacatttt catttatatg caggaaatga aagtcaagaa
    38881 gtattgctga aagaaatgga gaattggcca acattttatc cagatacaat ggatggacgt
    38941 gaaattgcac aggaaatgct tttgcactga taggcaaaaa agaaccgaag ggtttggatc
    39001 ggctggtcat ttagtcggtc caaactattc ggttcttttt atgaaatcaa tttctttaat
    39061 ccacggatag aggctgggat ggtctcgccg tttgccaacg tgacttctct ggttttttta
    39121 ttaatcgacc gcacattttg tgtattgata acataggatt tatgacaacg gataaaattc
    39181 cgatgcattt tctcgatttc tttgatcttc tcatagaatt gtaattgggc atgagtcaaa
    39241 tggacttcca gtttatgtgg cgctcctagc gaagtagaaa aatattggat gtctttgatc
    39301 ggtaaaaatt tgatctcatc attaaactct attttgaaga tatctttttg gatttgggac
    39361 atttgaaaat cttcgaaaat tttgctcagg atatgctcga cattttcttt taaatcataa
    39421 agatcttctt tgacgatata atcagtcggt tcgatgttgc tttttaagat tttcatcgac
    39481 atttccgtat gggaagtcac atagattatt tttcctaaag gatctaattc acggatcaat
    39541 ttaccgatcg ctacaccatc catttcactg tcttctagtt cgatatctaa aaagtaaata
    39601 ccaaaagaag aagttctgat cttggctgca ttgagaagtt cttccggatc agaggtagaa
    39661 atatggacat aactgtctaa gttttcaatc atgattctat ttttgatcac cgttgataaa
    39721 acttctaatt gttttggatc gtcttcacaa atataaatgg gaatcatatc attactcctt
    39781 tattttattt gtatataaat cgtttgagaa aaacgctggt tttcaattga tgtttcaagg
    39841 aaaagataag gattggtctt tgtcaattca tccaagataa acaagccaag gccttcgttt
    39901 tttggatttg tttttgttga gaaccctttt tgtttcaact ggtttaacgg taatgtattt
    39961 gttgttgtat tgatcacacg aatcatcacg gaatgattct tcttgagtat ggtcaattcg
    40021 atttttttct tctttgcagc actagctcct tcaatcgcat tgtccaagat aataccaagc
    40081 atacgtacaa ggtttaccgt atgtttcgta tccagttcga ttggctcagg aaaatgaagc
    40141 tggacgatca accctttttc ctgtgccatc atgagtttta atgacagtaa actacggatc
    40201 tcaggaactt tcatattatc caacatactt agattggccg gaaaaaggtt catcatctgg
    40261 cgtgtaggta atatcgtttg atcataataa gaccgtaatc cttctaagtc gccttctgaa
    40321 atatagctgt gaagagaaaa aagcagattt tggtaatcat gtttgaattc tctcaagtct
    40381 tgatagtttt tttctagatc tttgatataa tcctgttgca tcctgacttg ttgctctaat
    40441 aactgatcag ctaatttctt attagagatt ttccaataat aaaccaagat gatcaggcca
    40501 acacacagat atagaatgaa aaaactcgta ttcaaagcta agacatcagg tgtttcacca
    40561 gaaaaacggg tataaaaaat aatgatatag tatgtaaata atatcaatgt cccgatcgta
    40621 ccgatcatac ggcggtccac agtgtgaaac caagtctcta acgatttttt taaacaaaaa
    40681 gctaataaag cagataaaaa agcggataag acaatgtgaa aatactgcaa tgaatctcct
    40741 ggttcaataa ccgaagattt caaaacaaaa aaatcgccga tgcttgttaa gtgatctcct
    40801 aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca attgttgacg cgaagtcgaa
    40861 aagaagaaaa aagtaagcat cagcattcct atagcaatta gagcagtaaa aatccctaaa
    40921 atcgaataga cccatccaat caaaatacaa gaaatcagaa tgactcccgc aaacagatat
    40981 ttgtatcgga tgtgatggtc aatcaataag atacaaattg tgtaaatcaa atattgcaac
    41041 gctatggacc ataaaaacat aaagtacagc tcccattgat ttttaccaaa ttatagcaca
    41101 attggcgtat tattttttta atttttcagg gattttgaat tcagaactca taccaaagca
    41161 gcagccagat acaaaaaagt cagataagtt agttaattgt ttaatgattg tttttttcat
    41221 gaaagattcc tccaaaattc atcaagtagt ttgtaatagg aagcaacgta gctatttcta
    41281 aaacaatccc aagtagcaaa taggtagaaa agtaagaagc tgagaaaaat aacgtaatga
    41341 gagaaaaaat tagaaacatg ctgatcgcct tctttaataa tgggggacgt ttctcgggtt
    41401 caatgggatt gattgctgta cctcttgatc catagcgaag cagtaacaaa aagcagctga
    41461 ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg cagatgtttc atcaccaagg
    41521 gaacgcccca taaataaagt agtgtccaca gcagacaaac gaattcagag tcggaatgca
    41581 atccataagc atatctgcgg atgcagatat aagaaagaaa ggtgattcct gtttccgccc
    41641 atgagcctgt caccagagaa aagactagaa cgaccgctag ttttccaaga ttattcaata
    41701 aaacttctaa gacaaatttt acttggacat acaccatatc ttcttccgat ttgtctccgc
    41761 tgaataaggc ttctaataat ttgttcacaa ctttatcttt catctcatac gtacctccac
    41821 cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt ttcataaaaa gtggttttga
    41881 catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt ccaagaacct taccaaaaaa
    41941 agtgacagtt ttgatacgaa aagtactttt tatgacaagt ttgtgctttt tcgagtgatt
    42001 tatgttaaaa aattactgac cttataaaag gtgatggagg gattcttatg gaaaatgaac
    42061 aattgtttga agtcggcgaa acatatgcat ttaaatctga tttatttgaa tcaccaataa
    42121 aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat acgtgtcatg aaatgcgcag
    42181 tagaagacaa tgataccgct cataatctgc actggcagtt agttgtacgc aaatcaagta
    42241 caatagaaga ataaaaacag ataaaatgtt gagagcataa agaggttgtg agtctcccgt
    42301 ttagctctga gttacaagga ataatgttaa aaaacagctt ttcatgtttt atgaatattg
    42361 ccttgtgacc gaggtgttgg caggagaata ctgtttattt gaaaaaaggg ctgcgacaag
    42421 acttttgtca cagcctttat tttcatgaat aggagttact tttggctact tttagagaag
    42481 tagcttttaa ggagcagata aagtcaaggc ttttatttct ataaatgaag tagtaaaatg
    42541 aagaacagat tttattacag gaaggatgcc gataattgtg atcattgttg caggaacgat
    42601 cggtgcaggg aaaagcacac taactgaaat gcttgcccaa gatttagaaa caaaaccttt
    42661 ttatgagaac gtggaagata atgaagtatt gccgctattt tattctaatc cagaaaagta
    42721 tactttttta ttacagattt tctttttgaa caaacgcttt ttggcaatca aagatgcttt
    42781 cagccatgat gacaacgtac tagaccgctc catctacgaa gatagtatgc tgtttcattt
    42841 gaatgcagat ttagggcgtg tttctgaagt agaagtaaag cagtatgaag gtcttttaga
    42901 aacgatgctg aaggagttag aagaaatttc gccacaaaag aaaccagatc tactggttta
    42961 tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa aaaagaggcc gggaatatga
    43021 acagctagaa caagatcctg aactctattc ctactataaa gaactcaatc gacgatacga
    43081 agagtggtat gaacagtttg atatatgtcc caaaatagtg atcgatgggg acaaatatga
    43141 tttcgtagcc gatccggcat gtggagaaca aatcgtacaa gaaatcaaga tgcgggcaaa
    43201 gaaaatgaca gaagaggcag atgcccagtc gatccgctac actacaaata acccgaaagc
    43261 aaagggaatc tttacaacta aataatataa aaactaagca aattgctcac tcgcagatat
    43321 ttctctgtga gcagcatctt gcttagtttt tttgattcat tgagatcaaa tagtgaaccg
    43381 attcgtatga taaaattgcc ataaatgaaa tgtctgagtg agggccatga tcaaaaataa
    43441 aatggaaacg atcaaaatcg aatgggcgcc aaacagatga tgacagaaaa caaggacaat
    43501 agctcctgcg ataaaggcaa gaacgacact tagatagtgg aaaaatcgat caaaatctgc
    43561 tttttcttta gaaatacaat aatcatataa gttcgttgct acccctttta aatttccagt
    43621 tgtaaataaa ttagtgtagg cacgcccgtt gattttatca aaagctaccc actggatcga
    43681 tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc tcatggacgg gtgtcaaact
    43741 aacaaagaaa aaaatcactg cttcataata tagaacaaac aagcgccaga aatgtttttc
    43801 tttttttcta aaataatcaa ttagatactt tgttagaaga atccctaaga aaaagaaaag
    43861 ggtagacaat acttttttac caacacttag ccattctccg tcaaatgctt ggatgatcga
    43921 tagaatgata ttgccagttt gagcagaggc aaaagcacca tattggatat aagtataact
    43981 gtccatggct cctccgataa atgtcagcaa aagtcctatt gtacgcccct cgtgaaagga
    44041 agtctgcatt tttttcatca ccttttatga atatttttgt cttccttatc ttagcataaa
    44101 cgattgaaaa aaaaggaaaa attttcagaa aactaaaatc atatcgcttc ccattttcat
    44161 ttttagaaag gtctgttagt ataaagaaaa aatagacaac gaaactttaa ataaccaact
    44221 tgaaataaac aaattcaaag aagaaagatg ggaaggaaat caatcagttg aatgaaaaag
    44281 aaattctccg attacaaaaa gaattgaaac gctgggagaa caagtataaa aagcttcaac
    44341 aagtctttcg gctacaagaa gaaatcattg ctacctatga agcggcttat ggcgaagcac
    44401 ccggacttat tgaggaagaa gtacaagtaa aagaaagtga aggaaacagc tctttcttta
    44461 aaatgatgga gaaacgttcc tataatcgct tcaatcaaga acaaaaagca gctatcacat
    44521 atgatatgaa gaaacatctt cgaatcatag caggagctgg gagcggcaaa acgcaaacaa
    44581 tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt agacgaagaa cgcatactaa
    44641 tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa acgagtagat aatttcagcc
    44701 agagaaaaac cgctgtccat atcggaacat tccatagtat cttttttagg atatacaatg
    44761 aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat acaaggagac ttttcaaagg
    44821 atactgcaca aaaagtgaat gctgttttgc aacagctgat acgtaaatat aatctttata
    44881 tatttgatca atatggagaa aaaacgattg cttctcggtt agactattgg cagaatatga
    44941 acctttcagt agaagaaatg gttcagttag ttaaagaaaa atatgattcc attgataaga
    45001 atgcacgaca gccgatatca gaacgactgt atggattgct cacggaactc caagaacaaa
    45061 aaagaagcca gcaattgcta gagttcaatg atgtactcca aaatctgaaa agcgctttgg
    45121 agaatgagga gatccagcat tatatcggcc aaaaatatga ttatctattc attgatgaat
    45181 ttcaagatac taatccactt caatgggaaa tcgtgaaacg gatgacaaag gaaaacagga
    45241 tcaagttgat tgttgttgga gatgatgatc agagtatcta tggattcaga ggatcagagc
    45301 cagcatacat caagaacttt gaaaaagaat atcctaccaa aactcttttc ttattaacta
    45361 attatcgttc tcgggcagct attgtccaag gagcaaatag attgatcagc tataataaaa
    45421 atgatcggat accaaaggcg atgattccag cacaaaaaga agcaggtgtt atggaagcct
    45481 atttgttttc tgatactaaa gcagaaagtc agtggctaat caaacaaatt cagacattta
    45541 ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata ccgatcagct tcacaaacgc
    45601 agcagcttgt ccaatctctg ctgaaaacga atattccttt tgtactggaa gcagatagtc
    45661 catatgaagg tatctttggt atcaagtctt tccaacattt ctatcaaaaa attgttcatt
    45721 ggcaaacagc actgaatctg caaaagaaaa atcaggccta ccagcaattg ctccggcagc
    45781 tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca atacttttct cctaaatatc
    45841 aaaatatagc cgttgcagat tatattttgt cgataagccc caatttaaaa tcgaaatctg
    45901 cagaaatccg agagtttgag gaagcgttgc gtaaaacaga ccagcatcaa gaaatctatt
    45961 cgcttgttca aagctatctt catctgccac gtatagctaa ggaaatcgac cagagtgatc
    46021 aagaatggat acgagaagaa gtaaagcagc atgctacttt cagaagactc caagccttat
    46081 cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg cttgattgct tacagacaag
    46141 gaaaacttga tgcactatgt atccagagca ttcacaaaag taaaggtctt agttaccgaa
    46201 atgtcttttt gattggttgt aacgaaggaa gtttgcctta taacggagcg acagaaaaga
    46261 aagtaatcga ctttaatgaa ataaaagcag aaccagtaac gacgatagaa gaagaacgaa
    46321 gactatttta tgttgcgatg actagagcaa gaaatcgtct gtatctatgt gtacctcaaa
    46381 tgaaaaattc aagaaaattg aaagtatcga gatttatcaa agaaaccggg agtcgtgtga
    46441 aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg tcagtttcaa gaaagaagaa
    46501 ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt cagcttattt tcgaagaagc
    46561 tatttctgga acacagttta ttcggaaaag ggaggtgtga caagaatttt gtcacacctc
    46621 ccttatttat tttgaaaatg agaatactat tatcaaaata gagaatatat agaaaaattc
    46681 tcattattca aacgtaaata atcagttatt ttgatttttt ttatatttta ttagctaata
    46741 attggtattt taaaagaaat tactgtagaa ttggattata attaaaaagg agtgagaaaa
    46801 tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga gcatggttga
    46861 aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta acagaaagtg
    46921 agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt tccactcggt
    46981 cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag caaatgatcg
    47041 tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat gaaaaaagaa
    47101 tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa attcgatgtg
    47161 ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag ggagggagcc
    47221 ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa ggcgaaacaa
    47281 tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg cgatttaagt
    47341 acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca gctatttgcg
    47401 acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg aaaaaaacaa
    47461 ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat tattatcgat
    47521 gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt aaataaatag
    47581 actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag tcgaacactt
    47641 agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata gagacatgct
    47701 caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa tatattgaca
    47761 tgtgtaaatt tacatgatat attcatttct gttgttttaa gtagcaaagc aataaaatag
    47821 tattttaaaa aacatataaa atagttgttg acatgttaga aatgacatga taagataact
    47881 gagttgttaa aaaagaacca cttgtgaagc ttgattctat cgaaaaaaac ttcaaaaaag
    47941 ttcttgacaa ataacaaatg ataagttatg ataaatgagt tgctgtaaga tttcgaaaaa
    48001 cgaaatcaaa aaaactttta aaaaagagtt gacaattact tgtctctctg atatgatata
    48061 aaagttgcta cggcagccaa aaacgaatcg gggaacactc ctgatgaagt agacctttga
    48121 aaactgaaca aagtaagaca aaccaaatgt gtagggcgtc ttgattcaat tcaagacaac
    48181 aaaca
//
LOCUS       DAYYAR010000007        44665 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000007 DAYYAR010000000
VERSION     DAYYAR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.007"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..1012)
                     /locus_tag="ACHAT0_000330"
     CDS             complement(2..1012)
                     /locus_tag="ACHAT0_000330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8450305.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1192..1536)
                     /locus_tag="ACHAT0_000331"
     CDS             complement(1192..1536)
                     /locus_tag="ACHAT0_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8450306.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1529..1963)
                     /locus_tag="ACHAT0_000332"
     CDS             complement(1529..1963)
                     /locus_tag="ACHAT0_000332"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8450307.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2101..2838
                     /locus_tag="ACHAT0_000333"
     CDS             2101..2838
                     /locus_tag="ACHAT0_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450308.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2831..4042
                     /locus_tag="ACHAT0_000334"
     CDS             2831..4042
                     /locus_tag="ACHAT0_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8450309.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4246..5028
                     /locus_tag="ACHAT0_000335"
     CDS             4246..5028
                     /locus_tag="ACHAT0_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8450310.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5098..5760
                     /gene="trmB"
                     /locus_tag="ACHAT0_000336"
     CDS             5098..5760
                     /gene="trmB"
                     /locus_tag="ACHAT0_000336"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8450311.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5858..7612)
                     /gene="ade"
                     /locus_tag="ACHAT0_000337"
     CDS             complement(5858..7612)
                     /gene="ade"
                     /locus_tag="ACHAT0_000337"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8450312.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7744..8673
                     /gene="comGA"
                     /locus_tag="ACHAT0_000338"
     CDS             7744..8673
                     /gene="comGA"
                     /locus_tag="ACHAT0_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8450313.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8630..9679
                     /gene="comGB"
                     /locus_tag="ACHAT0_000339"
     CDS             8630..9679
                     /gene="comGB"
                     /locus_tag="ACHAT0_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8450314.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9676..9954
                     /gene="comGC"
                     /locus_tag="ACHAT0_000340"
     CDS             9676..9954
                     /gene="comGC"
                     /locus_tag="ACHAT0_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8450315.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9929..10399
                     /gene="comGD"
                     /locus_tag="ACHAT0_000341"
     CDS             9929..10399
                     /gene="comGD"
                     /locus_tag="ACHAT0_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8450316.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10371..10718
                     /locus_tag="ACHAT0_000342"
     CDS             10371..10718
                     /locus_tag="ACHAT0_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450317.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10681..11106
                     /gene="comGF"
                     /locus_tag="ACHAT0_000343"
     CDS             10681..11106
                     /gene="comGF"
                     /locus_tag="ACHAT0_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8450318.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11078..11437
                     /gene="comGG"
                     /locus_tag="ACHAT0_000344"
     CDS             11078..11437
                     /gene="comGG"
                     /locus_tag="ACHAT0_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8450319.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11609..12616
                     /locus_tag="ACHAT0_000345"
     CDS             11609..12616
                     /locus_tag="ACHAT0_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8450320.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12641..13825
                     /locus_tag="ACHAT0_000346"
     CDS             12641..13825
                     /locus_tag="ACHAT0_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8450321.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14262..15845)
                     /gene="abc-f"
                     /locus_tag="ACHAT0_000347"
     CDS             complement(14262..15845)
                     /gene="abc-f"
                     /locus_tag="ACHAT0_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8450322.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15950..16078)
                     /locus_tag="ACHAT0_000348"
     CDS             complement(15950..16078)
                     /locus_tag="ACHAT0_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450323.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16423..16566)
                     /locus_tag="ACHAT0_000349"
     CDS             complement(16423..16566)
                     /locus_tag="ACHAT0_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8450324.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16789..17268)
                     /locus_tag="ACHAT0_000350"
     CDS             complement(16789..17268)
                     /locus_tag="ACHAT0_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8450325.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17341..17625)
                     /locus_tag="ACHAT0_000351"
     CDS             complement(17341..17625)
                     /locus_tag="ACHAT0_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450326.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17703..17897)
                     /gene="ssrS"
                     /locus_tag="ACHAT0_000352"
     ncRNA           complement(17703..17897)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHAT0_000352"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17969..19255)
                     /locus_tag="ACHAT0_000353"
     CDS             complement(17969..19255)
                     /locus_tag="ACHAT0_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8450327.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19393..20064
                     /locus_tag="ACHAT0_000354"
     CDS             19393..20064
                     /locus_tag="ACHAT0_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8450328.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20030..20518
                     /locus_tag="ACHAT0_000355"
     CDS             20030..20518
                     /locus_tag="ACHAT0_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8450329.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20531..21478
                     /gene="prmA"
                     /locus_tag="ACHAT0_000356"
     CDS             20531..21478
                     /gene="prmA"
                     /locus_tag="ACHAT0_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8450330.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21479..22243
                     /locus_tag="ACHAT0_000357"
     CDS             21479..22243
                     /locus_tag="ACHAT0_000357"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8450331.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22476..24689
                     /locus_tag="ACHAT0_000358"
     CDS             22476..24689
                     /locus_tag="ACHAT0_000358"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8450332.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24700..25146
                     /gene="dtd"
                     /locus_tag="ACHAT0_000359"
     CDS             24700..25146
                     /gene="dtd"
                     /locus_tag="ACHAT0_000359"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8450333.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25317..26576
                     /locus_tag="ACHAT0_000360"
     CDS             25317..26576
                     /locus_tag="ACHAT0_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8450334.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26665..27612)
                     /locus_tag="ACHAT0_000361"
     CDS             complement(26665..27612)
                     /locus_tag="ACHAT0_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8450335.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28006..29298
                     /gene="hisS"
                     /locus_tag="ACHAT0_000362"
     CDS             28006..29298
                     /gene="hisS"
                     /locus_tag="ACHAT0_000362"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8450336.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29313..31082
                     /gene="aspS"
                     /locus_tag="ACHAT0_000363"
     CDS             29313..31082
                     /gene="aspS"
                     /locus_tag="ACHAT0_000363"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8450337.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31191..32066
                     /locus_tag="ACHAT0_000364"
     CDS             31191..32066
                     /locus_tag="ACHAT0_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8450338.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32332..34215
                     /locus_tag="ACHAT0_000365"
     CDS             32332..34215
                     /locus_tag="ACHAT0_000365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8450339.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34290..34574)
                     /locus_tag="ACHAT0_000366"
     CDS             complement(34290..34574)
                     /locus_tag="ACHAT0_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8450340.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34564..35298)
                     /locus_tag="ACHAT0_000367"
     CDS             complement(34564..35298)
                     /locus_tag="ACHAT0_000367"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8450341.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35427..36050
                     /locus_tag="ACHAT0_000368"
     CDS             35427..36050
                     /locus_tag="ACHAT0_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8450342.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36242..37363
                     /locus_tag="ACHAT0_000369"
     CDS             36242..37363
                     /locus_tag="ACHAT0_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8450343.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37370..40498
                     /locus_tag="ACHAT0_000370"
     CDS             37370..40498
                     /locus_tag="ACHAT0_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8450344.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40517..43711
                     /locus_tag="ACHAT0_000371"
     CDS             40517..43711
                     /locus_tag="ACHAT0_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8450345.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43857..44564)
                     /locus_tag="ACHAT0_000372"
     CDS             complement(43857..44564)
                     /locus_tag="ACHAT0_000372"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8450346.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14327 a         7939 c         9385 g        13014 t
ORIGIN      
        1 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
       61 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
      121 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
      181 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
      241 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
      301 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
      361 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
      421 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
      481 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
      541 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
      601 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
      661 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
      721 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
      781 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
      841 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
      901 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
      961 ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc attaaaattt
     1021 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
     1081 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
     1141 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
     1201 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
     1261 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
     1321 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
     1381 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
     1441 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
     1501 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
     1561 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
     1621 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
     1681 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
     1741 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
     1801 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
     1861 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
     1921 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
     1981 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     2041 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     2101 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     2161 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     2221 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     2281 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     2341 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     2401 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     2461 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     2521 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     2581 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     2641 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     2701 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     2761 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     2821 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     2881 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     2941 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     3001 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     3061 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     3121 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     3181 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     3241 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     3301 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     3361 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     3421 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     3481 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     3541 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     3601 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     3661 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     3721 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
     3781 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
     3841 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
     3901 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
     3961 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
     4021 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
     4081 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
     4141 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
     4201 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
     4261 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
     4321 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
     4381 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
     4441 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
     4501 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
     4561 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
     4621 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
     4681 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
     4741 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
     4801 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
     4861 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
     4921 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
     4981 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
     5041 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
     5101 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
     5161 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
     5221 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
     5281 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
     5341 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
     5401 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
     5461 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
     5521 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
     5581 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
     5641 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
     5701 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
     5761 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatccca gaaaatgaac
     5821 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
     5881 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
     5941 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
     6001 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
     6061 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
     6121 gctgattgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
     6181 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
     6241 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
     6301 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
     6361 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
     6421 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
     6481 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
     6541 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
     6601 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
     6661 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
     6721 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
     6781 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
     6841 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
     6901 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
     6961 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
     7021 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
     7081 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
     7141 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
     7201 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
     7261 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
     7321 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
     7381 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
     7441 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
     7501 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
     7561 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
     7621 ctcccaagat aaatttttat aagtataaca taaattcggt ttttgccaat atttttttgt
     7681 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
     7741 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
     7801 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
     7861 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
     7921 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
     7981 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
     8041 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
     8101 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
     8161 agcggaccag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
     8221 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
     8281 cagatcaatc cgaaaatagc acagacttat gatacacttt tgagactcgc attacggcat
     8341 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
     8401 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
     8461 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
     8521 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
     8581 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
     8641 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
     8701 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
     8761 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
     8821 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
     8881 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
     8941 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
     9001 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
     9061 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
     9121 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
     9181 gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
     9241 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
     9301 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
     9361 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
     9421 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
     9481 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
     9541 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
     9601 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
     9661 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
     9721 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
     9781 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
     9841 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
     9901 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
     9961 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
    10021 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
    10081 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
    10141 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
    10201 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
    10261 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
    10321 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
    10381 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
    10441 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
    10501 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
    10561 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
    10621 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
    10681 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
    10741 ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
    10801 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
    10861 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
    10921 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
    10981 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    11041 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    11101 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    11161 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    11221 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    11281 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    11341 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    11401 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    11461 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    11521 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    11581 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    11641 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    11701 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    11761 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    11821 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    11881 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    11941 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    12001 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    12061 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    12121 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    12181 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    12241 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    12301 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    12361 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    12421 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    12481 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    12541 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    12601 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    12661 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    12721 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    12781 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    12841 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    12901 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    12961 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    13021 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    13081 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    13141 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    13201 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    13261 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    13321 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    13381 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    13441 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    13501 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    13561 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    13621 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    13681 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    13741 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    13801 tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag atgaaacgca
    13861 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    13921 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    13981 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    14041 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    14101 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    14161 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    14221 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    14281 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    14341 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    14401 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    14461 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    14521 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    14581 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    14641 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    14701 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    14761 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    14821 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    14881 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    14941 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    15001 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    15061 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    15121 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    15181 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    15241 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    15301 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    15361 cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag
    15421 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    15481 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    15541 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    15601 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    15661 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    15721 gatttgccag ttccgttgat accgactaag gcaatttttt caccagcttc aatttgacag
    15781 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    15841 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccatg gcctgtgtta
    15901 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    15961 caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    16021 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    16081 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    16141 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agtttttttt
    16201 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    16261 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    16321 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    16381 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    16441 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    16501 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    16561 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    16621 cgactaatcg ccctggtgtt cacttttttt tattttactt gaactcattg actgttttcc
    16681 ctttatttta aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg
    16741 gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt
    16801 gtggtcttta gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc
    16861 tgcgtttcta ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa
    16921 tcctgtggct cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat
    16981 aatttgtttt ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt
    17041 cgcattgctt tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag
    17101 cattccatca aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag
    17161 aagttctccg ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc
    17221 atcagaacca tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc
    17281 ctcaagtcaa tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat
    17341 ttactgcttt ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat
    17401 aattacgatc agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat
    17461 acccattacg cctaaaacag catatgcagc tccgtaaaat tgtaggaatc cttgattttt
    17521 atcatttttg ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac
    17581 gaataataat actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa
    17641 aataagcaac aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga
    17701 tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag
    17761 gtgggtccca tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac
    17821 acaagacggg tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc
    17881 gaacatcaca gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga
    17941 gctcgcagaa acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt
    18001 ttatacggta atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat
    18061 aatactgagc attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa
    18121 aatgatgagg atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc
    18181 tatctctcaa atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg
    18241 catctaatgc catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt
    18301 ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca
    18361 ctgtacgagc cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca
    18421 gacgccggca aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg
    18481 cagcatccac atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag
    18541 catctccatt tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca
    18601 gtgtaagatg aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc
    18661 cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt
    18721 cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga
    18781 ctgccaactg gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc
    18841 ggatcgctgg attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc
    18901 ggccactttc taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt
    18961 catccaataa gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat
    19021 ccttttttga atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga
    19081 tcgcacttgc gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga
    19141 gcatgcgagc atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac
    19201 caacgacttc atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat
    19261 tttcctcctt cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa
    19321 gaacagcctt ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag
    19381 taggtgagaa aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg
    19441 ccagaagtgg cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga
    19501 ttagctggat atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat
    19561 agctatggtt taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc
    19621 tacctttaca cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt
    19681 cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa
    19741 aatcgtggcg gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca
    19801 ttagcaattc ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt
    19861 tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac
    19921 aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct
    19981 ttacaaagaa aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa
    20041 ggatctatta acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc
    20101 tttagattgg gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa
    20161 taaagcacaa tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga
    20221 atttgaagac ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta
    20281 tctagcagta atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt
    20341 agtggaatat ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga
    20401 tgaagacgat gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt
    20461 agtagaagta aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg
    20521 agtgacagat atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc
    20581 agttgctaat attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga
    20641 tgttgaacat tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc
    20701 gctgaaagaa ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat
    20761 cttgcctttt atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa
    20821 aaataaagta acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata
    20881 ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca
    20941 gacagaagat cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg
    21001 cactcatccc acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga
    21061 aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg
    21121 tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa
    21181 tatggatatg aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg
    21241 aatcgagatt gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat
    21301 gatcccagat gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat
    21361 tgaagagaag aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca
    21421 aattttccag caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat
    21481 gcagcggtat tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga
    21541 aaactatcac cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc
    21601 attcaatgat caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta
    21661 tctgaaagaa attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc
    21721 gagtggctat cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg
    21781 agcgcaagct tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa
    21841 acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca
    21901 tcggcagatc gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct
    21961 agatgattat gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc
    22021 aaatgcagcc aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg
    22081 accagaaggt ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact
    22141 ttgtggatta ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc
    22201 tgctagctat caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa
    22261 agagtatcga cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat
    22321 ttatacaatg gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa
    22381 aaaaacgtat aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc
    22441 cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg
    22501 gacccggtgt cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg
    22561 agaaagcatg cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac
    22621 cctacattat tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc
    22681 atacagtggc tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag
    22741 atctgagaga agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag
    22801 ggaaaataaa atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc
    22861 tagcaatggc tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca
    22921 tgcgtacgtt gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag
    22981 aaatctatgc gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag
    23041 atacagcact tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga
    23101 cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga
    23161 cagaagagct ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct
    23221 atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc
    23281 gagtgatcgt agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat
    23341 ggaaaccaat gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc
    23401 aatctttgca tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa
    23461 cacaggaaat gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag
    23521 gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga
    23581 tactcgaact tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag
    23641 atatctttag tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa
    23701 aaggatccgg accattggac tttgcataca gtatccatac agatatcggt aacaaaacca
    23761 ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata
    23821 tcattgagat catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag
    23881 ttgctactag caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag
    23941 aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc
    24001 ctaaagatat tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa
    24061 cagaagatga tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca
    24121 atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag
    24181 aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg
    24241 gcgttgtcat tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga
    24301 ttcctggtga tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc
    24361 gagattgtcc gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg
    24421 aatgggaaga tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct
    24481 ataatcgttc aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc
    24541 tcaacagcgt tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg
    24601 gtatccagaa tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg
    24661 tctacagtgt acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg
    24721 gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta
    24781 cttgttttat taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa
    24841 aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa
    24901 gatgtgggag gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa
    24961 ggaaatcgac caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa
    25021 tcattcaatg aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca
    25081 gatatgtcgg tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac
    25141 aaataaagta atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact
    25201 gataaactct aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc
    25261 ggaaaaaaag ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga
    25321 aagaagtacc agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat
    25381 acaacatgtt ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt
    25441 tcaataaaga agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa
    25501 ggaatttcaa acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag
    25561 gtggagtaca gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc
    25621 taatgacacc ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag
    25681 cacttgaagc acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta
    25741 catctatttg cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa
    25801 aattagcaga atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca
    25861 taaaaggtgt cgagaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag
    25921 ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca
    25981 cagatttaga tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca
    26041 tccgtccaac ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta
    26101 gaccagattt gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc
    26161 aagaaatgag acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag
    26221 tagaagtaga tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa
    26281 cagaccatga tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag
    26341 ggaaagcaaa agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa
    26401 attttcgaca aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt
    26461 tttccgatca gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc
    26521 cattcaatca gtttccgact tatccgaata gccgagcgct actttacaag aattagatta
    26581 gcataaactt atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta
    26641 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
    26701 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
    26761 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
    26821 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
    26881 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
    26941 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
    27001 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
    27061 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
    27121 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
    27181 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
    27241 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
    27301 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
    27361 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
    27421 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
    27481 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
    27541 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
    27601 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
    27661 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
    27721 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
    27781 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
    27841 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
    27901 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
    27961 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
    28021 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
    28081 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
    28141 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
    28201 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
    28261 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
    28321 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
    28381 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
    28441 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
    28501 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
    28561 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
    28621 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
    28681 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
    28741 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
    28801 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
    28861 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
    28921 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
    28981 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
    29041 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
    29101 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
    29161 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
    29221 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
    29281 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
    29341 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
    29401 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
    29461 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
    29521 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
    29581 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
    29641 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
    29701 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
    29761 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
    29821 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
    29881 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
    29941 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
    30001 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
    30061 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
    30121 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
    30181 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
    30241 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
    30301 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
    30361 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
    30421 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
    30481 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
    30541 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
    30601 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
    30661 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
    30721 tctatgcaga agcttatgat atcgtcttga acggttatga attaggcgga ggttctatcc
    30781 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
    30841 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
    30901 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
    30961 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
    31021 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
    31081 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
    31141 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
    31201 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
    31261 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
    31321 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
    31381 taccggtatc aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa
    31441 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
    31501 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
    31561 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
    31621 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
    31681 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
    31741 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
    31801 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
    31861 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
    31921 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
    31981 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
    32041 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
    32101 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
    32161 ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt
    32221 tatgggatga tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt
    32281 aatacccgta tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa
    32341 caaaaaaggt taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct
    32401 gtggtgggtg ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc
    32461 gttccgattt ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg
    32521 gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat
    32581 ccggcacttg tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg
    32641 ctgttaaaca atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg
    32701 caaatcaata acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat
    32761 atctggtctt tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg
    32821 ttgatggtat ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc
    32881 ctctccgcca tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat
    32941 tacggtaccg acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg
    33001 ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct
    33061 ggaggaatct cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat
    33121 ttcatctatt acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg
    33181 acagcacacc ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt
    33241 ggcaaacgaa gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca
    33301 aaggtgactg atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt
    33361 ctaatcatca gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat
    33421 ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa
    33481 ccatgggttg ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca
    33541 ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa
    33601 aaattagcag aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg
    33661 atccttgata agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta
    33721 acggcatttg gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca
    33781 aaagtagtgg tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa
    33841 aaattaaagg aacaagattt gttgaaagac actgttctga ttggactagg aacgaatggc
    33901 tcattctcgg aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac
    33961 tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa
    34021 cagatgacga aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac
    34081 catagtgact ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac
    34141 attcacttag tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca
    34201 aacgtgacag aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag
    34261 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
    34321 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
    34381 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
    34441 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
    34501 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
    34561 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
    34621 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
    34681 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
    34741 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
    34801 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
    34861 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
    34921 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
    34981 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
    35041 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
    35101 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
    35161 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
    35221 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
    35281 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
    35341 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
    35401 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
    35461 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
    35521 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
    35581 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
    35641 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
    35701 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
    35761 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
    35821 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
    35881 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
    35941 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
    36001 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
    36061 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
    36121 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
    36181 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
    36241 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
    36301 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
    36361 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
    36421 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
    36481 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
    36541 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
    36601 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
    36661 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
    36721 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
    36781 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
    36841 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
    36901 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
    36961 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
    37021 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
    37081 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
    37141 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
    37201 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
    37261 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
    37321 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
    37381 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
    37441 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
    37501 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
    37561 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
    37621 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
    37681 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
    37741 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
    37801 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
    37861 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
    37921 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
    37981 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
    38041 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
    38101 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
    38161 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
    38221 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
    38281 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
    38341 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
    38401 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
    38461 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
    38521 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
    38581 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
    38641 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
    38701 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
    38761 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
    38821 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
    38881 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
    38941 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
    39001 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
    39061 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
    39121 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
    39181 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
    39241 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
    39301 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
    39361 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
    39421 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
    39481 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
    39541 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
    39601 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
    39661 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
    39721 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
    39781 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
    39841 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
    39901 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
    39961 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
    40021 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
    40081 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
    40141 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
    40201 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
    40261 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
    40321 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
    40381 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
    40441 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
    40501 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
    40561 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
    40621 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
    40681 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
    40741 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
    40801 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
    40861 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
    40921 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
    40981 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
    41041 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
    41101 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
    41161 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
    41221 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
    41281 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
    41341 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
    41401 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
    41461 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
    41521 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
    41581 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
    41641 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
    41701 tggtgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
    41761 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
    41821 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
    41881 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
    41941 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
    42001 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
    42061 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
    42121 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
    42181 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
    42241 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
    42301 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
    42361 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
    42421 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
    42481 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
    42541 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
    42601 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
    42661 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
    42721 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
    42781 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
    42841 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
    42901 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
    42961 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
    43021 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
    43081 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
    43141 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
    43201 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
    43261 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
    43321 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
    43381 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
    43441 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
    43501 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
    43561 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
    43621 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
    43681 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
    43741 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
    43801 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
    43861 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
    43921 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
    43981 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
    44041 agcatatgaa attctttctc agccagctga aaaatctctt tttctggttt ttggaaacct
    44101 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
    44161 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
    44221 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    44281 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    44341 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    44401 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    44461 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    44521 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    44581 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    44641 aagctgataa ataaaggttt ttctt
//
LOCUS       DAYYAR010000008        40000 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000008 DAYYAR010000000
VERSION     DAYYAR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.008"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..1028
                     /locus_tag="ACHAT0_000373"
     CDS             159..1028
                     /locus_tag="ACHAT0_000373"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8450347.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1193..2464
                     /locus_tag="ACHAT0_000374"
     CDS             1193..2464
                     /locus_tag="ACHAT0_000374"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8450348.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2461..3756
                     /gene="rho"
                     /locus_tag="ACHAT0_000375"
     CDS             2461..3756
                     /gene="rho"
                     /locus_tag="ACHAT0_000375"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8450349.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3855..4118
                     /locus_tag="ACHAT0_000376"
     CDS             3855..4118
                     /locus_tag="ACHAT0_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8450350.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4563..5012
                     /locus_tag="ACHAT0_000377"
     CDS             4563..5012
                     /locus_tag="ACHAT0_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8450351.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            5069..6487
                     /locus_tag="ACHAT0_000378"
     CDS             5069..6487
                     /locus_tag="ACHAT0_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8450352.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6487..7347
                     /locus_tag="ACHAT0_000379"
     CDS             6487..7347
                     /locus_tag="ACHAT0_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8450353.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7521..8294
                     /locus_tag="ACHAT0_000380"
     CDS             7521..8294
                     /locus_tag="ACHAT0_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8450354.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8441..8806
                     /locus_tag="ACHAT0_000381"
     CDS             8441..8806
                     /locus_tag="ACHAT0_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8450355.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8954..10162)
                     /locus_tag="ACHAT0_000382"
     CDS             complement(8954..10162)
                     /locus_tag="ACHAT0_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8450356.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10181..10459)
                     /locus_tag="ACHAT0_000383"
     CDS             complement(10181..10459)
                     /locus_tag="ACHAT0_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450357.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10652..11308
                     /locus_tag="ACHAT0_000384"
     CDS             10652..11308
                     /locus_tag="ACHAT0_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8450358.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11750..12547)
                     /locus_tag="ACHAT0_000385"
     CDS             complement(11750..12547)
                     /locus_tag="ACHAT0_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8450359.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12544..13728)
                     /locus_tag="ACHAT0_000386"
     CDS             complement(12544..13728)
                     /locus_tag="ACHAT0_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8450360.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13753..15000)
                     /locus_tag="ACHAT0_000387"
     CDS             complement(13753..15000)
                     /locus_tag="ACHAT0_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8450361.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15215..16960
                     /gene="dnaX"
                     /locus_tag="ACHAT0_000388"
     CDS             15215..16960
                     /gene="dnaX"
                     /locus_tag="ACHAT0_000388"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8450362.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16980..17294
                     /locus_tag="ACHAT0_000389"
     CDS             16980..17294
                     /locus_tag="ACHAT0_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8450363.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17319..17915
                     /gene="recR"
                     /locus_tag="ACHAT0_000390"
     CDS             17319..17915
                     /gene="recR"
                     /locus_tag="ACHAT0_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8450364.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17979..18623
                     /gene="tmk"
                     /locus_tag="ACHAT0_000391"
     CDS             17979..18623
                     /gene="tmk"
                     /locus_tag="ACHAT0_000391"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8450365.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18638..18967
                     /locus_tag="ACHAT0_000392"
     CDS             18638..18967
                     /locus_tag="ACHAT0_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8450366.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18981..19919
                     /gene="holB"
                     /locus_tag="ACHAT0_000393"
     CDS             18981..19919
                     /gene="holB"
                     /locus_tag="ACHAT0_000393"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8450367.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19955..20779
                     /locus_tag="ACHAT0_000394"
     CDS             19955..20779
                     /locus_tag="ACHAT0_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8450368.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20772..21119
                     /locus_tag="ACHAT0_000395"
     CDS             20772..21119
                     /locus_tag="ACHAT0_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8450369.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21188..22060
                     /gene="rsmI"
                     /locus_tag="ACHAT0_000396"
     CDS             21188..22060
                     /gene="rsmI"
                     /locus_tag="ACHAT0_000396"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8450370.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22169..23290
                     /gene="dinB"
                     /locus_tag="ACHAT0_000397"
     CDS             22169..23290
                     /gene="dinB"
                     /locus_tag="ACHAT0_000397"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8450371.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23344..23946)
                     /gene="nrdG"
                     /locus_tag="ACHAT0_000398"
     CDS             complement(23344..23946)
                     /gene="nrdG"
                     /locus_tag="ACHAT0_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8450372.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24261..26450)
                     /gene="nrdD"
                     /locus_tag="ACHAT0_000399"
     CDS             complement(24261..26450)
                     /gene="nrdD"
                     /locus_tag="ACHAT0_000399"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8450373.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26776..27543
                     /locus_tag="ACHAT0_000400"
     CDS             26776..27543
                     /locus_tag="ACHAT0_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450374.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27533..29575
                     /locus_tag="ACHAT0_000401"
     CDS             27533..29575
                     /locus_tag="ACHAT0_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8450375.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            29856..30005
                     /locus_tag="ACHAT0_000402"
     CDS             29856..30005
                     /locus_tag="ACHAT0_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8450376.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            30016..31530
                     /gene="dltA"
                     /locus_tag="ACHAT0_000403"
     CDS             30016..31530
                     /gene="dltA"
                     /locus_tag="ACHAT0_000403"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8450377.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            31527..32732
                     /gene="dltB"
                     /locus_tag="ACHAT0_000404"
     CDS             31527..32732
                     /gene="dltB"
                     /locus_tag="ACHAT0_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8450378.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            32777..33010
                     /gene="dltC"
                     /locus_tag="ACHAT0_000405"
     CDS             32777..33010
                     /gene="dltC"
                     /locus_tag="ACHAT0_000405"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8450379.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            33013..34281
                     /gene="dltD"
                     /locus_tag="ACHAT0_000406"
     CDS             33013..34281
                     /gene="dltD"
                     /locus_tag="ACHAT0_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8450380.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            34422..35504
                     /locus_tag="ACHAT0_000407"
     CDS             34422..35504
                     /locus_tag="ACHAT0_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8450381.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            35504..36307
                     /locus_tag="ACHAT0_000408"
     CDS             35504..36307
                     /locus_tag="ACHAT0_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8450382.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            36377..37117
                     /locus_tag="ACHAT0_000409"
     CDS             36377..37117
                     /locus_tag="ACHAT0_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8450383.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            37128..38132
                     /locus_tag="ACHAT0_000410"
     CDS             37128..38132
                     /locus_tag="ACHAT0_000410"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8450384.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(38177..>40000)
                     /locus_tag="ACHAT0_000411"
     CDS             complement(38177..>40000)
                     /locus_tag="ACHAT0_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450385.1"
                     /translation="YNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMK
                     EIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQW
                     ITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKV
                     YLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFA
                     LALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCT
                     FQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTP
                     WIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQV
                     DFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILE
                     EAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIH
                     ALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQE
                     RFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        12831 a         7033 c         8291 g        11845 t
ORIGIN      
        1 gcgctattgg atattttatt ttttgcaaaa aacttcttga aaattttcat tttcattgat
       61 tttagtagaa aactaccgaa atgtttggta aaatagggtc gttggttaag aattgtctta
      121 acaaatttag ttttacaaaa cttaggagga attttattat gccagtagta tcaggagctg
      181 aatttttaaa agctgctcgt aaaggcggtt acgctgtcgg cggattcaac acaaacaact
      241 tggaatggac tcaagcaatc cttgaagcgg ctgaagcaaa acaagcacca gtacttatcc
      301 aaacatcaat gggtgcagct aaatacatgg gtggttacaa aatctgtaaa gatttaatca
      361 ctgatttagt tgaagcaatg aacatcactg ttccagttgc tatccactta gaccatggtg
      421 attacgaagc tgctttggaa tgtatcgaat taggctatac ttcagtaatg ttcgatggtt
      481 cacatctacc atttgaagaa aacttgaaat tagctaaaga cgttgttgaa aaagcacatg
      541 caaaaggtat ctcagttgaa tgtgaagttg gttcaatcgg cggagaagaa gacggaatca
      601 tcggtaacgg cgaattagct gatattgaag aatgcaaaca aatggttgct acaggtatcg
      661 actacttagc ttgcggtatc ggtaacatcc atggtcaata tccagaaaac tggaaaggat
      721 tagcttttga ccacttacaa gcaattgctg atgctgtagg tccagacatg cctttagtat
      781 tacatggcgg atcaggtatt cctcaagatc aaatcgaaaa agctatctca atgggtatct
      841 caaaagtaaa tgttaacact gaattccaat tatcatttgc tgcagctact cgtaaatata
      901 tcgaagctgg taaagataaa gaaggtaaag gattcgaccc tcgtaaatta ttagctccag
      961 gtaaagcagc aatcgttgaa gatgctaaat ctcatatcga ctggttcggt tcagctaaca
     1021 aagcgtaagc atccacataa cagatacgat atataggaat ggagaacgac ttttgaagtc
     1081 gctctccatt cttttttact taaaacggca gaccatgctc gtttcttttt tttatctatg
     1141 ataaaataca agcatatcca tgaaaaaaga ttaataaaca aggtggaaac agatgaaaaa
     1201 aatcgtaatc aaaggaaacc gacctttagc tggtgaagta acgatcagcg gtgcaaaaaa
     1261 tagtgcagtc gcattgattc cagctgcaat cttagctgat tccccagtga tcttggaagg
     1321 tgtgccggac atacaagatg ttcattcatt aattgaaatt ttagagatca tgggcgcaac
     1381 tgtccatttt tccaacaaca tactagaaat cgatcctcga gaagtcgtat cgattccgat
     1441 gccgcacgga aaaatcaata gtttgcgtgc ttcttattat tttatgggaa cactgcttgg
     1501 taaatttggc gaagcggtag taggcctgcc aggtggctgt tatttaggcc ctagaccgat
     1561 tgacctacac gtcaaaggat ttgaagctct aggtgctgaa gtgacaaatg aacatggtgc
     1621 catgtattta cggacagaaa ataaaggatt gcgtggcaat cggattttca tggacgtggt
     1681 ttcagtcgga gcaacgatca atgtgatgtt agctgctgta aaagcaaaag gacagacagt
     1741 aattgaaaac gctgcacgag aaccggaaat catagatgtg gcaactttac tgaataatat
     1801 gggggcaaaa gttcgaggag cgggtacaga tgtgatccgt atcgaaggag tcgaaacatt
     1861 acatggctgt cggcatttca tgattcctga ccgcatcgaa gcaggaacct atctagcatt
     1921 agcagctgca gtaggaaatg gtatcaaagt gaaaaatgtg atctttgaac atttggaaag
     1981 ctttatagca aaattgcagg aaattggtgt ccatatgaaa atcagcgaag atgaaatcga
     2041 agtttacccc tcgaaagaat taaaacccgc aaacatcatg acctatcctt atccaggatt
     2101 tgccacagat ctgcagcagc cacttactgc gttactattg atgactacag gcacttcaga
     2161 aatcatcgat acgatctatg cgaaacgtgt gaaccatgtg ccggaactag cacgaatggg
     2221 cgctgatatt accgtggaag gaaatatgat catcgtgaat ggtccaaata aacttcacgg
     2281 cacagaagtc gtggcttctg atcttcgggc aggtgcttgt ttagtgattg ctggactatt
     2341 ggcagaaggg acaacgacga tttacaatgt agattatatc ttacgcggtt atgatcatat
     2401 tatcgagaaa ctaacagcct taggggcatc aattgaaatg atagaggaag tgggagctga
     2461 atgagcgatt atttgacaat ggcagaacta gagaacaaaa cgttaaaaga agtttatagt
     2521 tatgccaaag aatttaaaat tccttattac agtaagatga ataaaaagga attatctcta
     2581 gctgtgattc gtgcacaagc agagaaacaa ggattttatt atatggaagg aatattggat
     2641 atcgttggtc aagatggtta cggtttttta cggccaatca attatggacc aagtgcagaa
     2701 gatatctata tttctgcctc acaaatcaga cgattcagtt tgagaaatgg ggacaaagtg
     2761 gcaggaaaag cccgtccacc taaagaatcg gaacgttatt atggtttgat gcacgtggaa
     2821 agcgttaatg gaaaagatcc ggaagaagca aaagaacgtc cgcattttcc agccttaaca
     2881 cctctttatc cgcaaagaca gcttcaactg gaaacaacac cgcaaagact atctacccga
     2941 atgatcgata tcttttctcc aatcggtttt ggtcagcgtg gattgatcgt cgcaccgcca
     3001 aaagcaggga aaacttctgt attaaaagaa atcgcaaatg gtatcaccga aaactatccg
     3061 gaagtagaat taatcgttct actgattgat gaacgaccgg aagaagtaac ggatctagaa
     3121 cgaagcgtga aaggcgacgt ggtatcttct acatttgatc tgcagccgca aaaccatact
     3181 cgagtttctg aattagtatt ggaacgcgca atgcgtttag tcgaagataa acgagatgtc
     3241 gttatcttga tggatagtat tactagactt gctcgggcct ataacttagt cgttccacca
     3301 agcggacgta cattgagtgg cgggattgac ccagccgcat tgtataaacc taagaaattt
     3361 ttcggggcag caagaaacat cgaagaaggt ggcagtttga cgattttagc gaccgcttta
     3421 gtcgatacag gaagccgtat ggatgacgtg atctatgaag aattcaaagg aactggaaat
     3481 ctagaattac aactttcacg tgaattatct gaacgtcgga tcttcccagc catcgatatc
     3541 aaaaaatcag gtacccgaaa agaagaatta ttgatgccaa gtgaagaatt agaagaaatt
     3601 tggaaacttc gaaaaaatat gactggagat tcattagaat atacggaaca actgatccgc
     3661 tttttgcgta aaacaaaaaa caatcagcaa tttttcaaag aatttcaaga tgtctccttt
     3721 ggtaaaaaaa atccgaccag aaatctgaaa agataattgc aaccttgact gaaacatgtt
     3781 aagatgttaa tgttgtaaat cgacaaatca actctggttc agcaaatgga gcagggcaaa
     3841 ggagcgaaaa aatcatgaaa gaaaacatcc atccagatta ccacccagta gtattcatgg
     3901 attcaacaac tggtttcaaa ttcttgtcag gttcaacaaa aacttcacaa gaaacagttg
     3961 aatgggaaga cggtaataca tacccagtca tccgtgtcga agtaacttct gactcacatc
     4021 cattctacac tggacgtcaa aaattcacac aagcagacgg acgtgtggac cgtttcaaca
     4081 aaaaatacgg tctcaaagac gcaaacgctg cggaataata aaaacgctga taactcaacg
     4141 tttagcagac tatctgatag ggatagtctg tttttttatg ttttttgcca atttaaaact
     4201 tcgtttgatg ctgaaaaagt cgtaggatca taatgcacaa aacaatgaaa aactagtata
     4261 tagttttaat tatccacttg ataaaaacac cttatattaa cgaaatgaag aggggttatt
     4321 tcgaagtaaa aaaccattta cctaaaatta gaagctaagc tttcctttca aggaaggctt
     4381 tttgttttgt tatagacttg cgattgtttg tgagctagat cgttcatttt aatgacttat
     4441 acacgcggat gatgggtctt tttcctctgt tcattcataa tattgaacgg tacagctagc
     4501 gatgctatac tgacctcagt tccaagaaaa cgttaacatt ttttgtgggg gagaggtttt
     4561 ttatgaggtt tgatcaattg atcgcagaag aactgattgt tttcgatcca tcgatagatt
     4621 caaaaggatc attatttgag cgaatagggg atgtgttgaa aaaaacaaat aaggtaagaa
     4681 atgttcaaaa agtcgttaag gcactattca aacgagagga ggagatctcc acaggaatag
     4741 aagatggatt tgggattccc catgcaaaaa gcaaatatgt gacaaaacca gcactgtgtt
     4801 tttttcatac agccaaatta acggactata gcgggataga tgaaatgcca attgaatata
     4861 tttttgccat cgtagttcct gaaaaatcag cagatatgca tctagagatt ctcagcacac
     4921 tctccagacg attgatgaat gcagatttta gaaagggaat aaaagaagcc actacgccag
     4981 cagatatcat cagacagatt agtcaagaat gaaaatagta gagtctttgt ccgagtatcg
     5041 atagtgaaga aaaaggagga aaataatcat gaaaatcgtt ggtgtaactg cttgcccgac
     5101 tggaattgct catacttata tgtcggcaga aaaattagaa gtaacagcaa gaaatctaaa
     5161 ccatcaagca aaattcgaaa cacaaggtgt aaaaacagaa aatcagttat ctgaacaaga
     5221 aatcaaagag gcagatgcaa tcattcttgc agtagataaa gaaatcgaat tggaccgatt
     5281 tgccgggaaa aaagtaaagc gtgtctctac aagccgagca atcaaagaac cgcaagtagt
     5341 aatagacgaa gcattgagag atattggcgt attcgttgtg tctaatgaaa aagaaccagc
     5401 tgcaaatgag aaaaagccga caatctataa tcatttcatg aatggtgtga attacatgct
     5461 tccatttgtg atcgcaggcg gaatcatcat cgcactgagt tttgcctttg ggattacagc
     5521 ggctgatccc aactcagcag actataatgt gttagcagct gcattttcaa ggatcggagg
     5581 agatacagcg tttgccatga tggttcctgc gttaggcgcg gggattgcta catcgattgc
     5641 tggaaaagca ggttttgccc caggaattgt tgccggatta ctggcctcta caggtggttc
     5701 aggttttctt ggcggaatga ttggtggcgt gctagcaggg tatatctgtg attttctggc
     5761 aaataaaatt agcgtaaaaa aagaattttc tgctatttat cagctgattg tcgtcccctt
     5821 tttatcgatc ttaattattg ggcttttgat ggtatttgtg attgaacaac cgatcacatg
     5881 ggtgttggat gctttgacaa attggctgaa tagcttggga aatacatccg ggctattgtt
     5941 cggattactt atcgggatca tgatggcagc tgatatgggt gggccgatca ataaatcgac
     6001 ttcgactttc tcgatcggtt tgatgtcagc tggagtaaat gcaccgattg ctgcttgtat
     6061 ggctgcagga atgacacctc ccctaggtat tgctttagcc actttattat tcaaacataa
     6121 atttaccaaa gaagaacgga cttcaggtca atcatgctgg attttaggcg cttcgtatat
     6181 tacagaaggt gctataccgt ttgcggtatc tgacccgatc cgcgtgatac ctagtttgat
     6241 gacaggttca gcagtcgctg cagggatttc catggcagta ggagttactt caatggcacc
     6301 tcatggaggg atttgggtga tgctaattcc aaatgttatg aacaatttac ctatgtatgt
     6361 cttggcaatt gcatcaggta cagtcactac agcctttatg cttggactat tgaaaaaaac
     6421 agtgaaagag aaaaaacaag acaagtggac gaaaaatgaa aatctaacaa atgaggtgga
     6481 agcgtaatgt tatatacaat gaaagagtta ttgaaagtgg ctaaagagaa tcaatttgca
     6541 gtaccagcct tcaatatttg cagctatgat atgatgaaag caatcatcga agaagcagag
     6601 agattagaag caccagtaat cttagaaatt catcctgatg aaatcgccta tttaagtgat
     6661 gagtttgttg cggcagtaag agcctatgct tatacaagta aactgccgat cgttatccac
     6721 ttggatcatg gcggtacgat agaagatgtc ttacgggcga ttcgaaacgg ctattcttcc
     6781 gtgatgatcg atgcgtcttt gaaatcattc gacgacaatg tacagattac gaaaaaagtg
     6841 gttgcgttag ctcatgcggt aaatgtctca gtggaggctg agcttggaac gattgggaat
     6901 aatggttctg ctgaaggagg tgctgaaaca atcatttata cagatcctga tcaagcgaag
     6961 atttttgtga aagaaacagg aatcgataca cttgctgtag caattggaac agcgcatggc
     7021 ttgtatccga aaaacagaac tccaaaatta aatatcgact tactgaaaga attgaaccaa
     7081 caaatcgatc ttccatttgt tttacatggc ggttcaggta atccggacgc agaagtcagc
     7141 gaagcagtaa aatacggagt tgccaaagtg aatttgagct cagatcttaa aagcgtcttt
     7201 ttcaatgctt tgagagaaat attgagtgaa aatccatcga tgtacgagcc gaatgcaatt
     7261 tatccagcag ctaataaaaa ggtacaagaa gtggtcaaac ataaaatgga aattttgaac
     7321 acagtaggca aggctagttt atactaaaaa taaaaatgtg tggtcaagag ggtatgacaa
     7381 tagtcttgtc ataccctctt aaccacacac gatgttcaga agtagttttt tcgaaaaatt
     7441 tcttcttatt tctcgaagcc aatcacttct gtcacaacct cttttttcct gttactatat
     7501 aaagtaaatg gggtgatttt atgttgaatg aagtggagaa tagacagcag gagattgtga
     7561 attatctaaa aaacaaccaa tttgcaagag tcaccgattt gatcgagtta gtaaattata
     7621 gcgaagccac gattaaacgt gaccttgttt ttctggaaaa aagtggattg atccgtagag
     7681 ttcgcggtgg cgccatgctt attgacaatc gaaaaattga cgtgccttac ctgatgaaga
     7741 tcaccaaatt agaagaagaa acagaaaaac gtaagattgc agatacagct tcttctttga
     7801 ttcgagatga tatggtctta ttcttggatt ccagtacaac ctctttacac ttagtcagaa
     7861 acctagcaag atttgatggt ttgcagatca ttacgaatgg catcgtcaca gctgctatgt
     7921 tgagtgaata tacaacggca aaagtatctg tattgggagg ggcaattgtt ccgaaacgag
     7981 caacaatcaa cggttcaaaa gcctataacg atgcattgtc atattatgcg gatttagcga
     8041 taatgagttg tcgcgggcta gatttcagcc aaggagtaac agaaactcat gaaggagagg
     8101 caatggtcaa aaaagctttt agaaaacaat cagaccggct tgccgtttta gttacacagc
     8161 aaaaaattgc acataaattc atgcatcaag ccattgcttt tcatgaaatc gattatctgg
     8221 tgacaagtaa agaactgaca aaagaacagt atgcgaaagt aaaggaacac catatcgaat
     8281 gtctgtacgg ataaattatt acgtgaatgt tctaagtggc ttcctcattt ttagatagga
     8341 ccttatcttt gctcgcttct ctcagccgat ggtataataa tgaaaaggtt atcttgttag
     8401 ataatcttac ttttttatcc aggagaaagg agtgcagaaa atgaaacatg tgaaggcgtt
     8461 ggtagtgaag gcaatcatga tctgggcgat tctgtgggtc gtgctgacag gtttatacgg
     8521 tgttagcttt atggattcaa cgattgttgg ggtaattatt gtagtcatga tctatgtgct
     8581 aggggatctg ttgatcttac gtaaagtagg gaacatcgca gcgacgatcg cagatgctgg
     8641 ttcagctgca gttatcttgt ggctctatct atattttatg aatgatacag tcgatatttg
     8701 gatgaaagta ttgattcctg ctctattgat cggtgtggca gaatggttct tccacaagtg
     8761 gttgctaagt caaggcatcg taccagatga acggaaaatg gaataaatca acagttaaac
     8821 tattcttatc gataaatgaa agctttaaaa ataaatagat gtttaattaa gaataatcat
     8881 aaactatttt tactcaaaaa accgctcaga ttattttcag atctgaacgg ttttttttat
     8941 ctattcatca agatcaaatt tcatttttca atgattcaat caattcgccc tctgcttcat
     9001 gactatgttt tcggccatta acaagataga gtgctgtaca tatcaatgga atgactccgg
     9061 caatcacata caatcctcta aatgaagtaa gagattttag catccccatc aaataaggac
     9121 caacgcctaa gcctaggtct aaaccaataa agtaggtaga taaagcaata ctgattcgat
     9181 gttcgcggac gatttttaaa caaacagcct gtccatttga cataaacgta ccataaccta
     9241 ggccgactaa tgctccagag ataagcaatg tcacactatt cgtcgttaca ctcagtaaaa
     9301 ataatcctgc tgttaagaat aaatagctag gatacatcac gtattgttct cctttgacat
     9361 cgaaaatcct tccagtcaat ggacgtgtaa atgtgatgac caaagcatat acgacgaaga
     9421 aaaatgaccc agcagcaact aagtggatta cttttgtata agaagataaa aatgataaaa
     9481 cacttgaata agaaagaccc atcaaaaagg caattcctgt aatgaaaagt gctttctttt
     9541 caataaaact attgatattc catgagcgaa gagatatttt ttgttcttca gataatggaa
     9601 tattttttac aggaaagaca aggcaggcaa tcgtaacaaa gaagaccagt acaattgaaa
     9661 accagataat gaaacgaaaa tcagtcatat tcagcaaaat cattcctaaa aatggcccaa
     9721 cagcggcagc taaactagta cttaatccat aataattgat gccttcccct tttttagatg
     9781 cagggatata tgcagtaacg atagcatttg tagctgtaga aactgtaccg tagccgaatc
     9841 cattcaagaa tcgaactaaa tacaatgccc cgatagttgg cgtataaaga tatgctaatg
     9901 ttgaaaggag atagaagatc gcgccgcctc ttaaggtaaa cttccgacca aatagttcta
     9961 actgtttccc catgaataag cgtgccagca atgttccgat gatatagatc ccagaagcaa
    10021 aacctgcttg gctcaagctg gcgtgtaagg tgtcttgcgc aatgactgcc atgatgacca
    10081 tcaacagata ataaatcagg tagacaataa aattgatcaa tgtgattcca ataaaatatt
    10141 tattaaataa tttcttttcc atgtatgtta tacattcccc ttaagctgaa tatgctttta
    10201 atttttgtat ttgaacttct acatcgtgat aattggatgg atagcgatca acaagttggc
    10261 gaataaataa atctcttgta gacataaaca gatttaaaaa ttcttcgcat tcgtttgttg
    10321 gattctcatc cactgctttg atcaattcga tcgagttttg atacgcatca tttaaaaaga
    10381 ctaaagaagc tttcatatca aatcttgttt gatatgctgt ttctaatcga ttaatgattg
    10441 tgttcatcca gtaattcatg ttttttccct ctattcttaa aaaattttac ttctgcagta
    10501 taacgaataa aaaaaaggaa aaaaatcgca taagtgatgg aaaaaggtac ttaacaaatg
    10561 ttaatatcat tagtaaaata cctttggact ttaaaaagtt ttaaagtact gtatgatctt
    10621 tttagatgaa agtttggatg ggggagcatc attggatagt agagtcttaa aggaatattt
    10681 ggataaaaat gattttccga ttatacgcaa aggatatcat aaatatttga cattcgaagg
    10741 gatggaagat tcccatacgt atatcttgaa agagggaacg gtaaaagcaa gtgttatttc
    10801 aaatgatggg agagaattta accttcgcta tatcaaagat cttgaaatcg tctctctttt
    10861 aaaagacgaa tattcacaat tcatcgattc gccgtataat attcgagttg aatcagaata
    10921 tgcagagttt tatcgaatca atcgtgtgcg cttttggcaa aatatcaacc aagacccaaa
    10981 gctacaacac tatgtaaaag agtattaccg ctatcgtctg ctttattcga tgaaaaaaat
    11041 gcagcaaatg ctattaaacg gaaagctggg agctgtttgt acgcaacttt acgaattata
    11101 tgatttattc ggtgtgcgga cagaagaagg gatgctgatc gatttttcga tcacgaatga
    11161 agaaatcgcc aaattttgtg gcatctcaac tgctagcagt gtaaacagaa ttttaaaaca
    11221 actgaaaaaa gatggtatta tcaccgttaa agaccatcat atcgttattt tgaatatgga
    11281 cttgttagaa gacaatatta tcttttgatg aacaaagagg ttgtgaaatc agcgttttga
    11341 cctgagtatt agagaaacaa acggaaaaat agttcctcat attttgccgt tttttgagct
    11401 aataccgagg gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc tgccgtttaa
    11461 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
    11521 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
    11581 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
    11641 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
    11701 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
    11761 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
    11821 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
    11881 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
    11941 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
    12001 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
    12061 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
    12121 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
    12181 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
    12241 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
    12301 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
    12361 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
    12421 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
    12481 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt
    12541 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
    12601 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
    12661 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
    12721 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
    12781 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
    12841 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
    12901 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
    12961 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
    13021 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
    13081 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
    13141 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
    13201 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
    13261 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
    13321 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
    13381 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
    13441 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
    13501 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
    13561 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
    13621 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
    13681 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
    13741 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
    13801 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
    13861 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
    13921 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
    13981 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
    14041 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
    14101 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt agccttcacg
    14161 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
    14221 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
    14281 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
    14341 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
    14401 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
    14461 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
    14521 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
    14581 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
    14641 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
    14701 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
    14761 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
    14821 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
    14881 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
    14941 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
    15001 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
    15061 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
    15121 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
    15181 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
    15241 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
    15301 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
    15361 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
    15421 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
    15481 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
    15541 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
    15601 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
    15661 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
    15721 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
    15781 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
    15841 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
    15901 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
    15961 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
    16021 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
    16081 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
    16141 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
    16201 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
    16261 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
    16321 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
    16381 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
    16441 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
    16501 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
    16561 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
    16621 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
    16681 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
    16741 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
    16801 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
    16861 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
    16921 actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
    16981 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
    17041 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
    17101 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
    17161 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
    17221 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
    17281 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
    17341 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
    17401 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
    17461 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
    17521 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
    17581 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
    17641 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
    17701 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
    17761 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
    17821 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
    17881 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
    17941 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
    18001 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
    18061 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
    18121 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
    18181 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
    18241 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
    18301 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
    18361 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
    18421 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
    18481 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
    18541 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
    18601 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
    18661 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
    18721 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
    18781 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
    18841 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
    18901 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
    18961 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
    19021 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
    19081 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
    19141 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
    19201 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
    19261 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
    19321 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
    19381 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
    19441 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
    19501 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
    19561 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
    19621 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
    19681 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
    19741 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
    19801 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
    19861 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
    19921 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
    19981 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
    20041 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
    20101 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
    20161 agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc
    20221 aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt
    20281 tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt
    20341 ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg
    20401 cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc
    20461 ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt
    20521 gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag
    20581 cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc
    20641 tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct
    20701 gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt
    20761 aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt
    20821 tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa
    20881 aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc
    20941 aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc
    21001 ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg
    21061 acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac
    21121 caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt
    21181 gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct
    21241 tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca
    21301 agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt
    21361 tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca
    21421 attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc
    21481 ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt
    21541 gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc
    21601 tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt
    21661 gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac
    21721 tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga
    21781 attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat
    21841 cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat
    21901 gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat
    21961 cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa
    22021 aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata
    22081 atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat
    22141 gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga
    22201 tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg
    22261 agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg
    22321 tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc
    22381 aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca
    22441 tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat
    22501 tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg
    22561 caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct
    22621 aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca
    22681 aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt
    22741 gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg
    22801 gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg
    22861 aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt
    22921 tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga
    22981 agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag
    23041 agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt
    23101 gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc
    23161 aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac
    23221 tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca
    23281 agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg
    23341 tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt
    23401 ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt
    23461 gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac
    23521 aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc
    23581 aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg
    23641 tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa
    23701 tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa
    23761 tggtttgcca tatcgaaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa
    23821 gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt
    23881 gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt
    23941 tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct
    24001 actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct
    24061 tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac
    24121 ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag
    24181 cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc
    24241 aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg
    24301 cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact
    24361 acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc
    24421 ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca
    24481 agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt
    24541 tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc
    24601 ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg
    24661 tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa
    24721 cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca
    24781 tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa
    24841 gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac
    24901 aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa
    24961 tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct
    25021 ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag
    25081 cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt
    25141 ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt
    25201 tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt
    25261 aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc
    25321 aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc
    25381 aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt
    25441 cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat
    25501 cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc
    25561 aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg
    25621 cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct
    25681 gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac
    25741 gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat
    25801 tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag
    25861 cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct
    25921 ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg
    25981 atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg
    26041 ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct
    26101 ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat
    26161 tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt
    26221 tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca
    26281 taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg
    26341 atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat
    26401 gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac
    26461 tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc
    26521 ggctatatat agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata
    26581 ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat
    26641 tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa
    26701 cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa
    26761 agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc
    26821 agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag
    26881 tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc
    26941 acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg
    27001 aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc
    27061 aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg
    27121 atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa
    27181 gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct
    27241 gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg
    27301 gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa
    27361 gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta
    27421 tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc
    27481 tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta
    27541 taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag
    27601 tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt
    27661 agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat
    27721 cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag
    27781 gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat
    27841 atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg
    27901 tattattttt tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac
    27961 tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat
    28021 ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag
    28081 ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt
    28141 actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag
    28201 ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc
    28261 ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg
    28321 gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca attttttgat
    28381 aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt
    28441 cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc
    28501 ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa
    28561 aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta
    28621 taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa
    28681 tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat
    28741 ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa
    28801 tatttcatta tatggatcta gtattggact gccagataat caagaattga agattcaaca
    28861 aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt
    28921 agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt
    28981 atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg
    29041 gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag
    29101 cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg
    29161 tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa
    29221 tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac
    29281 agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct
    29341 ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt
    29401 tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta
    29461 tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta
    29521 tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac
    29581 ggaataagac aagataataa atagaactaa attttgaata ccatttattt ggaataagag
    29641 gttgtgacac gatttttgtc acaacctctt tattttttat attttttaaa agtacgagaa
    29701 attataaaga aaaaagcagt gatttgatgt gctattttta taaaagctat gtgacaaaac
    29761 tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt tctttttgtc aaaattagtt
    29821 acactttgtg tgaggttata tgaggaggga atccaatgaa caaatggcgt tctaatccaa
    29881 aattgaatta ttggggaaaa ttcatcattc gaacaatttt ttatagtgca gtactgctgt
    29941 tcctgattta tctgtatcac tataaaaata ttcaaggagg aacttttatc tataacgaat
    30001 tttaggagga agagtatgga aatcaaaacg attattgaag caatcgatga ttggggaata
    30061 aatgaacctg atcgagttgc ttatcaggcg gatgagacgc atacttttgg tgaattgaaa
    30121 caagcatcag acgcacttgc ttattattta gaaaaaaagg tagaaggcga tggaccaatt
    30181 gtcgtttttg gcaatttgga gtttgaaatg attgtctcat ttctaggggt tgtcaaatct
    30241 ggccatgcct atttgccgat tgaagagcat acacccaagg agcgtatcct atcgatcttt
    30301 cgggtggcta aaccatctat ggtcatcagt atcggggact ggatagaaga tattccttcg
    30361 gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga agataccagt aggattttat
    30421 cccagaaata gcgtaaaagg tacagaaaat ttttatatta tttttacttc tggtactact
    30481 ggagaaccaa aaggcgtaca aatcagtcat gataatctag tcagctttgt tcaatggacg
    30541 atggaagact ttgggatcaa gcctggtatg catttcttag cccaagcgcc attttctttt
    30601 gatttatcgg tattttctat ttatcctgca ttagtttctg gcggaatgct gaagccgtta
    30661 aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc cttgaagttg
    30721 aatgtgtggg tttctactcc atcatttatg gatatttgct tgatggaacc tacattcaat
    30781 ggagaaaatg tgccggaatt ggacatgttt ttattttgcg gagaagaatt gacgaaaaaa
    30841 actgcagaaa gtctacttga tcgattccca aatgcacgga tatataatac ttacggtcca
    30901 acagaagcaa ctgtagctat ttcaagtatt cagattgacc agcatgtact agatagttat
    30961 gaccgactac cgattggcta cgtaaaaaaa gacacgaaag tttctattgt ccaagaaggt
    31021 aaacctgttt caaaaggaga aacgggtgaa atcattattg caggaccaag cgtatcaaaa
    31081 ggttacctga acaatcccga aaagacagct gctgcgttct tttgtcaaaa tgacttagca
    31141 agttaccata caggggatgc aggtagacta gatgaatatg ggcttttatt ttatgaagga
    31201 cgtatggatc agcaggtcaa attacatgga taccggattg aactcgggga tatcgaacat
    31261 tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata ccaaggaaca
    31321 aaagtacagc agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca gccggatttt
    31381 cagttgacta aatctatcaa agaacaatta ctagaagttg taatggatta tatgattcct
    31441 cagaagttcc attatgtcga tctgctgcct cagacagtta atggaaaaat cgatcgaaaa
    31501 aaattaacag ctgaggtgaa tcctgaatga tgttacctca tatgatccct tatgcagagc
    31561 cgttttattt ttttctttta gccattgcgc ttgtaccgat cgttctatct ttattgatat
    31621 gggaaaaaag attaccagtt tatcaatcag cagtgacact tttcttttta tttgtcagct
    31681 ttggaggtaa ttattggcaa cagggagcgg cgctgattgg gtatgttttg tggcagacga
    31741 tacttgtatg gggctatttt gtttatcggc aaaggaaaaa tcagacagga tggttttttg
    31801 ccgccgttat tttagcgata attccactgt ttttaacgaa agtgactcct ttttttgaac
    31861 atggaaaagc atcgttactt ggttttttag gaatctctta tttaaccttt aaatctgtac
    31921 aaatgatcat ggaaataaga gatggcatga tcaaaacata taagccattt cgttatatcc
    31981 agtttctgct gttctttcca acaatctctt ctggaccaat cgatcgctat aggagatttg
    32041 aaaaagatca gctccatccg cctgttcgtg ataaatatgt tgatcttttg ggaaaaggcg
    32101 tccaaaatat ttttgtcggt tttttatata aattcattat cggttattat tttggtcaag
    32161 tactgcttcc tgtagtggga cgcattgcct tacagcatgg tggtatttca tgggcattag
    32221 taggttatat gtacgtgtac agtatgtatt tgttttttga ttttgctggc tatagcttat
    32281 ttgcagtcgg tacaagctac atgatgggat atcagacacc aatcaatttc aataaacctt
    32341 ttttatcatg gaatatcaaa gaattctgga atcgttggca tatgacgctg tccttttggt
    32401 tccgtgatta tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa ttttttaaaa
    32461 gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt tcttctaatg ggtatatggc
    32521 atggattgac gtggtactac atcgtatatg gactgtatca tgcttgtctg atctgtctga
    32581 cagatgcatg gatccgattt aagaagaagc ataaagagca gttgccttct aacaaattta
    32641 cgcatggctt agccgtattt ttgacatttc aagcagtatg ttttagcttc ctgatttttt
    32701 caggattttt agataaactt ttttttagct aacgaatagt gacatattaa tttatataac
    32761 aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa taacaggaac
    32821 agatgaagta agaaaaaatc gtgatattga tctatttgat gaagggttgc ttgattcttt
    32881 agcatctgtt caattattag tcgagctaga cggagagctg gatatccaag tccctgtatc
    32941 tgaattcgag cgagaggatt ggtgcacacc gaataaaatc attcaacaag caactgcatt
    33001 gaagggttga gtatgaaaaa gaaacttttt gctattttcg gcccggttgt tttagctgtt
    33061 ggcttactgt ttgtgttttt tacctcctcc tatcaagtag atatctctaa tccagaaacg
    33121 attagacgtg cctcttcttc catgtcagtg aacgtgttga aaggagatgc gataaaaaat
    33181 tatgcattat ctgaaaaaca atatattcct ttttttggct caagtgaatt aagcagaatc
    33241 agtccatttc atccgtctgt tctggcagaa aaataccagc gaaactatcg tccatttttg
    33301 ctgggagcac ccgggacaca atcgttaagc caatatatga tgatgcgttc tgcaggagat
    33361 gctatgaaaa acaagaaagt cgtctttatt atttctcctc agtggttcgt taaaaatggt
    33421 gtaaaaactg attactttaa tacgtattat tctgaacttc aaacatacga ctggttgttt
    33481 tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta gaagattatt aactttctca
    33541 aaagtaaaag agaatgatac gttgacggcg attctgcaaa cgatcaaaaa agggaagcta
    33601 cctttacctg aatctttgaa tcagctgcgc agccaatgga acatgctgaa gagagaggat
    33661 gaagtattca gcaatattgg gttgaaagat cgacagcaaa aaatcgatca tgagtcaaaa
    33721 cgattaccaa aacaatacca agaaacagaa ttatccattt tagcaaatca aattggagaa
    33781 cgagaaacga cgaataatcc attcgggttg aaaaacgatt tttatacgca tcgtatccga
    33841 gcccacgagc cagagctcaa acagtcacaa aaaaattggg attatcgttt ttcgccagaa
    33901 ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa accataacga agtattgttc
    33961 atcatcccac cggttaacga gaaatggagc gattataccg gactatctca agaaatgtta
    34021 caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa tcgaatagct
    34081 gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca tctaggctgg
    34141 aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa tcatattact
    34201 gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca tcaaatacca
    34261 gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt ttctttattt
    34321 ttgaatattc tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa tcataagcaa
    34381 tggtataatc acaatatgaa tatagaaatg ggggaaaaca tttggataca aaagaagaac
    34441 agctacttat tgatttaact tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag
    34501 aagtattcag agaatatgcc aaaccattcg ctgatgatat tttttatgac ggtttaggca
    34561 gtgtgattgc caaacatggt gctggcggag gccccaaggt ctttatttct ggacatatgg
    34621 acgaagttgg ttttatggta acaaaaataa ccgaaaaagg attcttggaa ttccaaactc
    34681 ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag
    34741 ggaaaatcgt ccacggtgtg attggttcca aaccaccaca tgttttaaca cctcaagtaa
    34801 gaaataaacc ttatgaaata aaggatatgt tcattgatat tggcgcatca agccaagaag
    34861 aagccaaaga gtggggcata cgtcctggag atatggtcac tccatatatt gaatacaaac
    34921 gaatgaatgg cagtaaatat ttactggcaa aagcatggga taatcgtatc gggacagctg
    34981 tttctctgcg agtattagag aatctatcta aagaagcaca cccaaatgtt ttattcgcag
    35041 gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa
    35101 atcccgatat tgcttttgct ttagatactg gtacagcagg agacactcca ggaatgacac
    35161 caaaagaagc agattcgatt ttaggaaaag gcccacagat tttgattttt gatgcatcta
    35221 tgatcccgca taaaaaatta ttgaattttg ttattgatgt agcagaggaa atggatattc
    35281 cattccaata tacagtgatt tctggcggag gaactgatgc aggacaaatg catttgacaa
    35341 gagatggtgt tccgtctgta gccattaccg ttcctgttcg ttatctgcat tcccatacat
    35401 cagtgattca tgaagatgat tatttgaata cagtgaaact agtcacagaa ttagtgaaac
    35461 ggcttgatag tgaaacagtt gaaaaaattc ggagttatga ataatgaata atagaatacc
    35521 tatttctgaa tatcggaaaa aagcacgagt ttctttggaa aatcaatggg gaatcaatac
    35581 ttggcttgtt ttcttatcta taatatttac gagcgtcatc cagaactttt ttgacggttt
    35641 cagtcctttt ccggaagaaa gcatgcagca gcacttattg agttttctag tcgataattt
    35701 acttttattc gcattcaact atgcattgtt ttacattgcg ctagtagttg tacggggtgg
    35761 cagaccaaaa agtaaccttt tattttcagt tttccaaaga aagtattttg gttcgatact
    35821 tggcatcaat ttgatcaatt cagtcatcaa ctggctactc aacagtttaa tctttctgcc
    35881 catgttttta atcagcggag tgagtactta tatgcagctg cttttcagtt caaatgcggt
    35941 acctacgggt gtcatgccag aagattttct tgatatcagc tttgttttat cgatgatagg
    36001 gatgattttt gtttcagcac tcatcatgag tattgttggc ggtttattcc aatttgctgc
    36061 atggacaaag ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat atggctggtt
    36121 tttattgaaa gatagaattg gtacgtatat tttgttgcag ctgtctttta tcggctggta
    36181 tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt
    36241 gtcagttgca gaattttatg aacaagcacg gattgaaaaa ggaggtccag acgcctattt
    36301 tacttagcat gtaagcagtt tattcatcaa aaaaataact tttgtggtag gataaatctt
    36361 tgagggggaa tgtacgatga aattaactgt tttaggatgc ttaggagcgt atccttataa
    36421 aaatcaaggg acaacaggtt atttgcttca atctgacggg ttcaatttgt tgatcgatgc
    36481 aggtagtgct acgctgatca aattacaaga acaccttgat ccacttgatc tagatgctgt
    36541 gatccttagc cattatcatc atgatcatat tgctgacctt ggtgtattac agtattactg
    36601 gcaacttcat cctaagagaa atccggagac attgctttca atttatggac acacaaaaga
    36661 ttccattcac tttgaagaat tgacgttgga aaatgtctca aaaggacatc cttattttga
    36721 agctgaagaa ttaaaaattg gtccttttct gattactttt atggaaacaa tccatccagt
    36781 agtatgttac gccatgcgta tcgtagaaga aacaacgggg aaagtattcg tatttacagg
    36841 tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc
    36901 agatacctat ctttttgaag gaaatgaaca tcatcatgcc catttcactt caaaagaagc
    36961 tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc tacctcagtt
    37021 tggtgattta gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat
    37081 agaacttgct gcagttgaca aagtgtttaa catttaggag gaagaacatg atttttgtac
    37141 caaatgaaaa ccacgaccca agaatcaatt tagcaattga acaatatttg ctgcaagaaa
    37201 tgcctttaga tgaacctatt ttgctctttt atatcaatga accttcaatc atcatcggca
    37261 gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat ggcattcatg
    37321 ttgtccgtcg cttaagtgga ggcggagctg tctaccatga ttttggaaac ctgaacttta
    37381 gttttatcat gcctgatgat ggggattcat tccgtgactt tgcgaaagta acacagccaa
    37441 tcatccaagc acttcatgaa ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc
    37501 tagtcattga tggcatgaaa ttttctggca acgcgatgta tgctaccaac ggtcggatgt
    37561 ttgctcatgg aacaattatg ttcgactctg atatcaatga agtagtcaat gcattaaaag
    37621 taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata
    37681 tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa
    37741 tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac gaattgactg
    37801 atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat tgggattgga
    37861 attacgggaa gtctccagat ttcaatttct ctcgccaaaa acgtttttct atcggttcga
    37921 ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt
    37981 tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag aaatatgata
    38041 aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag
    38101 aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa tccacatata
    38161 tggattccgc tattttttat acaggattcg ggtaatttcg agagagttga acagtgatcc
    38221 gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc gctacgtctt
    38281 tcatcatcat taagtcaaaa cgttcctgat catctaattc ttctgtgatg cctcgactaa
    38341 ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt tcctcttgtt
    38401 cgtgtatcga caaataaatt tttcgataca gtggattttc gatggcatga gtttctctaa
    38461 aggaatgctc taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg ataggaagtt
    38521 cttcgggaag atctgcttgg aaggttatct gaatattccg tttggcagct aacttctggt
    38581 aacgcgctaa gataagattt gtcacctggt ggttgcaaaa agaacacagc atttcgaaat
    38641 tttttgcttc ttctaatatc ttttccacat agtttttagc ttttgtttgg tccgtatgca
    38701 ttaaatcttg cagattctcc atctcttgga caaattccag gcgaagtgta tttacttctt
    38761 ggatatgctg tcgaagctgc tcataatgtt gctccaacaa atcatttttg atttttaaga
    38821 aatctacttg tctccgatct ttgtcggtat ttaagatgtg atcgattaga ttgtagctga
    38881 taataacgat tagaatgata atatataaaa tcgtagacca attgatcgtt gtttgataga
    38941 tacctaatgc cgtttggatg taaatcaagc aatggataat ggctaccaag acgatccaag
    39001 gtgtacaagc tacataaaaa cggttattct tatatgcttc gccaatgcat gcaagtgaag
    39061 tgactaaagt gcttataaca ctagcaatat tagcaatcgg cattaattca ggaagttctg
    39121 caggagtaaa agttgcataa agcagaacgc cgataaaaat caaaagttgg aacgtacaaa
    39181 aaattccgta ccatttttta tagtaaaaca ttcggctcca atagtagcag atgatgaaag
    39241 ctgacgttaa gataataaat aaatgagata aaaaagaatg aacgaatggt tcaaaaagaa
    39301 taccacttaa aatatcttct gatacagctt caaatcctaa tgccaaagca aagcaactta
    39361 agagaataag tgagtattca agacgttttt gtttatacat caagtagatc gtatagaaaa
    39421 aggtccctat agcgataaaa acagcgatta tcatcgtcaa tacttgcggc actgattgtg
    39481 aataaataaa actaatcaat ggtccagttg ttcctagata aactttcggt ggcagtccag
    39541 catagttctg ataaggagat gagactgtaa gggtcaattt ttttccgata taatttgctg
    39601 gtaaatctat cacagccaat gagagtccgg gattgctttc ttcctgataa tctttccgtt
    39661 ggtataaaat cttttcgcct agatatacag taatccattg atggtttcct ttgattagta
    39721 aatttgcttc gtccaacggt ttttcaagtg tcgtttccat aatgaatgtt tcgtctttat
    39781 ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt aaaatcagga tcatcttttg
    39841 tatagaaaac ccacgaatct tttatttctt tcatttgatt gctgtccgca ggagaaaaaa
    39901 taaaataaaa gaaataagca attcctgtta aaaataacaa tgataatata atgattatgc
    39961 tccactttat acgtaaataa aaagattctt ttttattata
//
LOCUS       DAYYAR010000009        39413 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000009 DAYYAR010000000
VERSION     DAYYAR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.009"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..205
                     /locus_tag="ACHAT0_000412"
     CDS             <1..205
                     /locus_tag="ACHAT0_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450386.1"
                     /translation="ENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIFLFPP
                     FIFIFAGGVWKLVSIILTIGNER"
     gene            227..1738
                     /locus_tag="ACHAT0_000413"
     CDS             227..1738
                     /locus_tag="ACHAT0_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8450387.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            1750..2709
                     /locus_tag="ACHAT0_000414"
     CDS             1750..2709
                     /locus_tag="ACHAT0_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8450388.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            2795..3874
                     /locus_tag="ACHAT0_000415"
     CDS             2795..3874
                     /locus_tag="ACHAT0_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8450389.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(4126..5526)
                     /locus_tag="ACHAT0_000416"
     CDS             complement(4126..5526)
                     /locus_tag="ACHAT0_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8450390.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            5744..7405
                     /locus_tag="ACHAT0_000417"
     CDS             5744..7405
                     /locus_tag="ACHAT0_000417"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8450391.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            7362..8060
                     /locus_tag="ACHAT0_000418"
     CDS             7362..8060
                     /locus_tag="ACHAT0_000418"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8450392.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            8076..8597
                     /locus_tag="ACHAT0_000419"
                     /pseudo
     CDS             8076..8597
                     /locus_tag="ACHAT0_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            9088..10053
                     /locus_tag="ACHAT0_000420"
     CDS             9088..10053
                     /locus_tag="ACHAT0_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8450393.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            10075..10287
                     /locus_tag="ACHAT0_000421"
     CDS             10075..10287
                     /locus_tag="ACHAT0_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8450394.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            10362..10838
                     /locus_tag="ACHAT0_000422"
     CDS             10362..10838
                     /locus_tag="ACHAT0_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8450395.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            10835..12643
                     /locus_tag="ACHAT0_000423"
     CDS             10835..12643
                     /locus_tag="ACHAT0_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8450396.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            12720..12935
                     /locus_tag="ACHAT0_000424"
     CDS             12720..12935
                     /locus_tag="ACHAT0_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8450397.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            13027..13890
                     /locus_tag="ACHAT0_000425"
     CDS             13027..13890
                     /locus_tag="ACHAT0_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8450398.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            13927..14463
                     /locus_tag="ACHAT0_000426"
     CDS             13927..14463
                     /locus_tag="ACHAT0_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8450399.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            14477..14899
                     /locus_tag="ACHAT0_000427"
     CDS             14477..14899
                     /locus_tag="ACHAT0_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8450400.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            14829..17228
                     /locus_tag="ACHAT0_000428"
     CDS             14829..17228
                     /locus_tag="ACHAT0_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8450401.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            17260..19251
                     /locus_tag="ACHAT0_000429"
     CDS             17260..19251
                     /locus_tag="ACHAT0_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8450402.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            19274..19525
                     /locus_tag="ACHAT0_000430"
     CDS             19274..19525
                     /locus_tag="ACHAT0_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8450403.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            19515..20744
                     /locus_tag="ACHAT0_000431"
     CDS             19515..20744
                     /locus_tag="ACHAT0_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8450404.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            20741..22822
                     /locus_tag="ACHAT0_000432"
     CDS             20741..22822
                     /locus_tag="ACHAT0_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8450405.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            22971..26891
                     /locus_tag="ACHAT0_000433"
     CDS             22971..26891
                     /locus_tag="ACHAT0_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8450406.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            26892..27836
                     /locus_tag="ACHAT0_000434"
     CDS             26892..27836
                     /locus_tag="ACHAT0_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450407.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            27840..28043
                     /locus_tag="ACHAT0_000435"
     CDS             27840..28043
                     /locus_tag="ACHAT0_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8450408.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(28343..28579)
                     /locus_tag="ACHAT0_000436"
     CDS             complement(28343..28579)
                     /locus_tag="ACHAT0_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8450409.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(28762..29133)
                     /locus_tag="ACHAT0_000437"
     CDS             complement(28762..29133)
                     /locus_tag="ACHAT0_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8450410.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(29130..30458)
                     /locus_tag="ACHAT0_000438"
     CDS             complement(29130..30458)
                     /locus_tag="ACHAT0_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8450411.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(30419..30748)
                     /gene="mobC"
                     /locus_tag="ACHAT0_000439"
     CDS             complement(30419..30748)
                     /gene="mobC"
                     /locus_tag="ACHAT0_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8450412.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(31006..31377)
                     /locus_tag="ACHAT0_000440"
     CDS             complement(31006..31377)
                     /locus_tag="ACHAT0_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8450413.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            31522..31707
                     /locus_tag="ACHAT0_000441"
     CDS             31522..31707
                     /locus_tag="ACHAT0_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8450414.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            31865..32032
                     /locus_tag="ACHAT0_000442"
     CDS             31865..32032
                     /locus_tag="ACHAT0_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450415.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            32650..33309
                     /gene="vanR-B"
                     /locus_tag="ACHAT0_000443"
     CDS             32650..33309
                     /gene="vanR-B"
                     /locus_tag="ACHAT0_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8450416.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            33312..34655
                     /gene="vanS-B"
                     /locus_tag="ACHAT0_000444"
     CDS             33312..34655
                     /gene="vanS-B"
                     /locus_tag="ACHAT0_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8450417.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            34826..35632
                     /gene="vanY"
                     /locus_tag="ACHAT0_000445"
     CDS             34826..35632
                     /gene="vanY"
                     /locus_tag="ACHAT0_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFM8450418.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            35650..36477
                     /gene="vanW-B"
                     /locus_tag="ACHAT0_000446"
     CDS             35650..36477
                     /gene="vanW-B"
                     /locus_tag="ACHAT0_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFM8450419.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            36474..37445
                     /locus_tag="ACHAT0_000447"
     CDS             36474..37445
                     /locus_tag="ACHAT0_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8450420.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            37438..38466
                     /gene="vanB"
                     /locus_tag="ACHAT0_000448"
     CDS             37438..38466
                     /gene="vanB"
                     /locus_tag="ACHAT0_000448"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8450421.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            38472..39080
                     /locus_tag="ACHAT0_000449"
     CDS             38472..39080
                     /locus_tag="ACHAT0_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8450422.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(39146..39319)
                     /locus_tag="ACHAT0_000450"
     CDS             complement(39146..39319)
                     /locus_tag="ACHAT0_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450423.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT        10657 a         9467 c        10280 g         9009 t
ORIGIN      
        1 tgaaaacaaa gatatcacat ttttacaaat ttttgaacaa agtgcatgga aagtattttt
       61 tgattttttg agaaatattc tttgttgcgt attgttatta ggatttgtac tgctcatatt
      121 tcttttccct ccttttattt ttatctttgc tggtggcgta tggaaacttg tatctattat
      181 cttgactata ggaaatgaaa ggtagggaaa gctgttggac gaagaagtga aagtaaaaat
      241 cagagaggat tttacggtag gagagatcag ttctcggctg tttggctcat ttgttgagca
      301 tatgggaagt gtcgtctata cagggattta tgaaccgggc catgaaacag ctgatggtga
      361 cgggtttaga caagatgtcc tctcttatgt aaaggaatta gaattaggtg tcattcgcta
      421 tcctggagga aactttacat ctggttatga ttgggtggat actgtaggta ggaaagaaca
      481 acgaccaaaa aaaattgatt tagcatggcg aggtatagaa ccaaattctt ttggactcca
      541 cgaatttttt aaatggatca gtcatgtaca agcgaaaccg atattcacgg tgaatttagg
      601 aacaaaagga attgatgaag caaaaaattt agtagaatat tgtaattttc caaaaggaac
      661 cttctggagt gatttaagaa taaaaaatgg gcaaaaaaag ccttttggcg ttaaattatg
      721 gtgtttaggg aatgagctag acggtccatg gcaagtgggg gctaagagtg cttatgaata
      781 tggtaggtta gcaaatgaag cctcaaaagt catgaagtta gtggatgatt caattgaaac
      841 aatcttagtt ggaagctcta cgccacgttt accatcgtac cctgaatggg atcgtatcgc
      901 attagaagaa gcttatgaga atgtagatta tttagctttg cataactata ttgataagta
      961 tgatgaagaa gacttgacta aacctcctaa aaatgagcgt gatgatacag cgacatacct
     1021 tgcaaaagct tatcgattcg atcgtcaaat tgaagaaatc gttgctactt gtgattatgt
     1081 aaaaggatat ttgagaagtg agaaaacaat gtatctagcg tttgatgaat ggaatgtaca
     1141 tgcccaaccg gaaaaaagac atagggattt tgaagtcggt agtcccatcg attggtgtta
     1201 ctttactatg gaagatacat tattatttgg ttcattaggg ctggctattt tacgacacgc
     1261 agatcgcgta aagattagtt gtcaatcatt gttagtaaac acaatacctt taatactgac
     1321 agatgctaaa ggacaagcat ggaggaatcc aacttattat gtgatgcaac acttatcgaa
     1381 atatgctaaa ggtcaagtat tgaaacaaca aatcatttgc cctgtttatg attgtgaaaa
     1441 gataaaaaat gttccttatg tggacagtgt gatcgtagat aacggcaatc agctgactat
     1501 tttcttgatc aatcgcacaa atgaacaaca aaagataacg attgaacatg atttcaattt
     1561 gctagatcaa ggagagcact acacattaac cagtagcgat ctgttggaca aaaatacacg
     1621 tgaagaacca gaaaaaataa aacctgaaat aaactatttt tcaatagaaa aacatgacga
     1681 attaaatgta gggatagaaa aatactcttg gaatgtaatt agaataaaga aaatgtgagg
     1741 aaatttgtta tgatgaagac actttataat aatcctatcg ttcggcaacg ggcagatcct
     1801 tgggtatata aacatacaga tggttattat tattttacag gatcggttcc aggctaccaa
     1861 gtgattgaat taagaagggc aaagtcactg aatgaattag aacatgctgc aagcctgatt
     1921 gtatggcagg ctcatgaaga aggaccgatg agtgaattga tctgggcacc tgaagtccat
     1981 tatatcaatg gaaaatggta tatttacttt gctgcaagca atcataaaac tatacgtgat
     2041 gaatctcatc atcaccgcat gttcgtactt gaaaatcagc atatagatcc aatgaataca
     2101 gattggatag aaaagggaca aataaaaacg aaaagggaaa ctttcagttt agacggaaca
     2161 actttctctt ctcaaggaaa gctatattat gtgtgggccc aacaagatcc tgaaattact
     2221 gggaattcta atctttatct atccgaaatg gataatcctt ggacgttaaa ggggaagcag
     2281 atcctccttt ctatacctga gtatgaatgg gaaaaacggg gtttttcagt aaatgaaggt
     2341 ccagcagtcc ttactcgcaa tgggaaaata ttcattacgt attctggtag tgcaacagat
     2401 gaaaactatg ccatgggcct gctgtgggct gatgagcaca agaatctttt attaaaagaa
     2461 tcatggcata aattatcgga gccagtattc gtttcttctg aaaaaaataa gcaatttggt
     2521 cctggtcaca actcgtttac ggtttcagaa gacgggtgct ttgatatttt gatctatcat
     2581 gctcgaccag agaagaacca aaaaggggat ccgttagaca atcctaatcg gcacgcgaac
     2641 gcgcaatgct ttacgtggaa tgagcaagga tttccagaat ttggtgaacc tgttccgtat
     2701 gccttatgag cacaagaaac cttgattttt gcttgaataa accttgcaga ctcgttatga
     2761 tatatgtacg tacaaataaa aaggggcaag gcaaatggca aaatatcaag atattgcaga
     2821 agagataaaa caaaagatcc ttcgtgagga atacgaagta aatacaacca ttccctcaga
     2881 attcaagctg caagaaatat atgacgtcag cagacatacg attcggcaag caatcgctgt
     2941 gttggtaaat gagggctatc tgcgtaaaga aaaagggtcg ggcacatatg tagatgacag
     3001 ctataagaaa gaaaaaacac cattgaacag aacaaaaacg atcggtgtca tcaccacata
     3061 tctctctgat tatatttttc cgtcgattat ccgaggaatc gaaaatgaat tacgagaatc
     3121 tgggtattca ttgcttttag ccagtacgaa taacgatcac gaattggaga aagagtgttt
     3181 gaagaaaatg attgcttatg gggtagatgg tttgatcgtt gagcctacga aaagtaatca
     3241 gtataaccca aacttagcac tttatgttgc tttgagagaa caaggcattc cgatggtgat
     3301 gatcaatgct gcttatgaag agttaaatgc ctcttatatt tgtttagatg atgtgaaagt
     3361 cggttttatg gcagcagaat atttgataaa aaaaggacat cagcgtctgt tgttcatcac
     3421 aaaaatcgac gatcttcaag gaaaatacag gatgaaaggt tttatcagcg cgtgtgaaca
     3481 gtataatgtg cgttttttga cggaagatat cattactttc accacggaaa cacgagaaac
     3541 aatcgctgaa aaaacagtac aacggttgaa agaagaacaa gctttagatg gcatcatttg
     3601 ctataacgat caaatagcta gtttaatgct agataagttg acagaaaatg gctatcatat
     3661 tccagatgac ctatcgatcg taggaacaga cgattcttct ttaagccatt tagggaataa
     3721 aaaattgaca acattgacgc atccaaaaga aaagttggga caagatgccg cacggtggat
     3781 cgtcacaacg attgaaaaag gacaggcaca gccaaatatt ctctatgaac ctgtactagt
     3841 tgaacgggaa acagtgaaag aaaacgaaaa ataagagacg ggtttttacc gcctcttatt
     3901 gctcctacgg ttactcgtcg caatgtttgt gagagctttt gtcatcgctc aaggctgaac
     3961 gtttctttca ccacctctcc actaaacggt gttctcctac caactggtca caagtcacaa
     4021 tattcatcaa acatgagaag ctgttttttg aaattgttcc ttgtgactgg aaactaagcg
     4081 gttccttctt atttcttgaa gctgaccact tctgtcacaa cctctttaag aatgatggat
     4141 agaaatacgt ttaggataag tttttttaga tgcactttgt tcgatttcct ctaaagattt
     4201 cccacgagtt tctggtacga tctcttgaat aaataaaacc cctaaaaagc agatgatccc
     4261 aaaaatgccg aaaacgattt cttgagacat agaagcagtc atcacaggaa aaagcaagcc
     4321 aactaggaaa gaaccaatcc aattaaaaga agaggctagg ccagatgctc gcccgcggac
     4381 agctagtggg aatatttcgc ctatgattac ccatgtcagt ggagcccatg tgcaagaata
     4441 aaatgcgaca taaatacata aaaacaagag aatcaacaac gaatttgtat gagcatccag
     4501 tacagttcct aaaacagctg gtaggatgaa tgaaagtccc atcacgctac cgcctatttt
     4561 caataaagtt cgccgattga acttgtctgc aataaccaaa aagagcaaag aaccagcaac
     4621 taaaatgatt ccttggatga tcggccacat caatgcatcg ctcgctgcat gcccagtcgc
     4681 tttttctacg atcagcggaa tatagtaaaa aatagcatta gctccttgaa attgctggaa
     4741 tactgctaca ccaataccgg caatgactaa ggaacgatat ttttctaaaa acaaagtatg
     4801 ccaagaagtt ccggttgtct tttcttgttt gacattcagc tggatttgtt cgaattcctg
     4861 ttcagcttct tttggtgtcc gaatatagtt caatactctt tttgcctctt ccaagcggcc
     4921 agattgcata agaaaacgcg gtgactctgg tagagctaaa acaccgaaaa ataaaatcag
     4981 cgcggggaca gcagcgagcc ctaacatcac gcgccaagcc atcgtttcag gcaacacttt
     5041 tagcaaataa tctgcaacat aggaaaggag catgccagaa gcaatcatca cttgattgat
     5101 ccctgacaat cttcccctta gtctagcagg agccatttca gacatataag caggaactag
     5161 ggcagatgca gcgccaacag ctaaacctag aagaatcctt gctgcaatca gaaaataaga
     5221 accattgtga ggggcagatg cagaaaggac agaaccagcc ataaaaataa gtgcagaaag
     5281 aagaatcatt tttcgtcgtc caagcttatc agaaagaaga ccagataaag agccgccaag
     5341 gatcgcacct aacatgaccg aagacgtgat ccatcctatc aaagaagctt tacctgccaa
     5401 accccaatca tgctgtaaaa aaggtaaggc gccggtcata acaccaatat catatccaaa
     5461 tagaatgccg ccaaatgcgc caaaaaaata aataaacgat gaggaaactt ttttttcttt
     5521 tttcataagt attctctcct tctagaattt cgagaaggcg gttgatcaga ttttgtaagc
     5581 aatttcattt tataacttat gtacgtacaa aacaagtgtt ttttattgta ttcttgtaaa
     5641 taaccgcttg acatgtacgt acaaaagtat acaataatca ttgtaagcgc tactaaaaag
     5701 acggtttgat caggtttgac gagaaggagg gagttaaatc attatgaagg aagtaaatgc
     5761 gcaaagtatg ttagatgtca tagatggtcg tacatcttta gggattgagt ttggatcaac
     5821 taggatcaaa gctgtattga ttgattcatc ttttactcct attgccgctg gtagttatga
     5881 gtgggaaaac aaactagaaa atggtatctg gacctattca ttagaagata tttggaaagg
     5941 actgcgcgtc agttaccgaa aaatggcagc agaagtattc gaaatctatg gcgagaatct
     6001 tacgacaatc gggtcgattg ggttcagtgc aatgatgcac ggttatctag catttgatag
     6061 caaggaacag ttactagtac catttcgtac ttggcgaaat acaatcactg aagaagcaga
     6121 acgaatatta acggaaaaat ttcaatacaa tattccgcag agatggagta tcgctcattt
     6181 gtatcaatct attttgaata aagaagaaca tgtgaaagaa attaattttt tgacaacgct
     6241 tgcaggttat gtccattggc agcttactgg tgagaaagta ctaggcgtag gagatgcatc
     6301 aggcatgttt cctatcgatc cttcaacgaa aacctaccat caagaaatga aaaatatttt
     6361 tggtcagtta atcgaaaaag cgcacgtagc gattgacttg gatcacattt tgcctaaagt
     6421 tctagtagca ggagaagaag ctggcaagtt aagcgaatca ggggcttatc tgatcgaccc
     6481 tacgggaaat ttgagaccag gaatcccggt ttgtccgcca gaaggggatg caggaaccgg
     6541 tatggtcgct acgaacagtg tagccaaacg gacagggaac atttcggctg gtacttccgc
     6601 cttcgctatg atcgtattag aaaaagaact agaaaatgtc catccggaaa tcgacatggt
     6661 cactacacct tcaggagaac tagtaggcat ggttcatacg aataactgct cttcggatat
     6721 caatgcttgg gtcagtattt ttgaagaatt taccaattct ttgggtatgg agataagcag
     6781 agataaatta ttcacggtat tattcaacaa agcgctagaa ggcgatacgg attgcggggg
     6841 attactttct tatggttatt tttccggtga aaacattaca ggagtaaatg aaggaaggcc
     6901 attatttgta cgtacaccag gtagccgttt tgatttagcg aatttcatgc ggatccattt
     6961 agccagtgct tttggtgcaa tgagaatcgg aatggatata ttgaaatctg agaatgtcca
     7021 aattgatcgt ttagtggggc atggggggat ttttaaaaca ccagaagttg gacagcggat
     7081 tctagcatct gctatggagg caccagttac agtgatggac acagccggag aaggtggagc
     7141 gtggggaatc gcgttattag cagcctatat gatggatcga catgggaagt ctttagaaaa
     7201 cttcttggca gaagacgtgt ttggacaaga ccaaggaatc accatcacac cttcagcaga
     7261 agaaatagca ggctatcaga tatttatgag acgttaccgt gaggggatcg acattgaaaa
     7321 aacagcgatt catcaattgc aagaaaaagg agtgatagaa aatgctggaa caactgaaag
     7381 aagaagtata ccaagcaaat cttgacttgc ctaaacacgg actcgtcaaa tatacatggg
     7441 gaaatgtgag cgcttttgat cctgaaacac gatatttcgt tatcaaacct agcggagtca
     7501 gttatgaaga gatgacagca gacgacatgg tcgttgtgga cttggacaat catatcattg
     7561 aaggtaaact caatccctct tcggatacgc ctactcatgc tgttttatat cgttcatttc
     7621 ctcaaatcgg aggaatcgtc catactcact cgacatgggc gacgatctgg gcacaagcag
     7681 gattggatgt tcctgcgatg gggacgacgc atgcagatac tttttatgga tcggtacctt
     7741 gtgctcgatt tttgacacaa caggaaatcg acagcgggta cgaatatgaa acaggaaaag
     7801 ttatcataga aacattcaaa gaacgcaaaa ttgatccctt agcaattcca ggggtacttt
     7861 tacacggaca cggtccattt acttggggga aagatgcgca aagtgcggta atgaacgcag
     7921 tcgtactaga tgaagtatgc aagatgaatt tatttaccag acagctcaat tcattttcag
     7981 aagagctgcc tcaacggatt ttagataaac attatttacg aaaacacgga aaaaatgcgt
     8041 attacggaca aaacaaataa aaggagtggc taattatgtt gaatattgga gaaaaagaat
     8101 tttggtttgt tgtaggatca cagcatttat atggggaaga agcactgcga gaagtaaaga
     8161 aacatgcaca agaaatggtc gatgaactga atgaaaatgg ccaattgcct tatccaatcc
     8221 gccttcaaga attagcagtt actgctgata cgatcacaaa aatcatgaaa gaggtcaatt
     8281 atcgagaaga agtagcaggt gtgatcactt ggatgcatac tttctcacca gcaaaaatgt
     8341 ggatccgcgg gacaaagctt ctacaaaaac cattgcttca tttagctacg caatataacg
     8401 aaagcattcc ttggaaaacg atcgatatgg atttcatgaa tttaaatcaa tccgctcatg
     8461 gtgaccgcga atatgggttc atcaatgctc gattaaataa acaaaataaa atcgtcgttg
     8521 gctactggaa acgacctgag atccaaaaag agattgctga ttggatggat gttgcagtag
     8581 cctataatga aagttttatt ccaaaaattt aggttatcgc tgaaagcccc ggaaatacgg
     8641 gcatttcgca ggatatgaga actcaattta ctaccaattt actactttcg gattgagccg
     8701 catatatgct gaaccatact gctaataccc gccaccccaa atacggcaca tcataaattg
     8761 aatacgacac cgtaaacgcg gcgttcctct ttcccgtcat gggagaacag gacgccgctt
     8821 ttttatgccc aaatagagaa aggaggcgac gccatgctat ttacttccgg ccctgaccgg
     8881 gcgtttgaac ggctgatgca ggaaaagccc ggtttcgatc actacgacgg cggcggggcg
     8941 gacgcgccgg aggattgcgg cacctgccga ttttaccgcc cggattggaa atatcaattc
     9001 tgccagtttg cagagtgtcc ctatcagccg ggcaagctga ccgactatgc agcggtcaaa
     9061 ttcacagtga aaggagcgaa caacgctatg gcagttttcc gtgtggaaaa gaacaaaggc
     9121 tatacggtga tgtcaaatca ccacctgcgg aataagaacc tgaccttaaa ggccaagggc
     9181 ctgctgtcgc agatgttgtc cttgccggag gattgggact ataccctaaa aggactgtcc
     9241 tatatcaacc gggaaagcat cgacgcgatc aggacagcgg tatgggagct tgaaaaagct
     9301 ggatatatcc ggcgtgagca gggccgtgac gcaaaaggca aaatggccga tatggtctat
     9361 accatctacg aacagccggt attggaaaac ccggtattgg aaaatccaac atcggataac
     9421 ccggtgttgg aaaatccgac atcggataat ccggcgtcgg aaaatccaac gcaattaaat
     9481 aaagaagtac aaagaactga cttatcaaaa aaagaaaaaa taattacaga tggacaaagt
     9541 acccattcca ttcctatccc atccccgacc cctgccccta tggagggcga ggcagcggct
     9601 acgccgccgg aacggaaaag gacaggaaag gatgaggctg tacggattta tcgggaaatc
     9661 attttggaga acatcgagta tgcctatctc attcaggaca ggagcattga ccgtgaacag
     9721 cttgacgaaa tcgtagacct gatgctggaa accgtctgca ccgcccgaaa gacaatccgt
     9781 gtcgccgggg acgactaccc tgccgagctg gtgaaatcca agtacatgaa gctgaccggc
     9841 gaacatatcc gctttgtcct tgactgtctg cgggagaaca caacgaaagt ccgcaatatc
     9901 aaacaatacc tgcgggctat gctgttcaac gcccccagca ccatttccaa ctactacacg
     9961 gcccttgtcg cgcacgacat ggcacagccc gattggggaa agcctaaatc cggcctgccg
    10021 gactattcct gcggcgaggg cgaaagcctg taacgagaaa aggaggacga ccctatgaag
    10081 cagggcgcat tgatttttga cgagcgaaca gaccgttacg acatccgctt tgatctggcc
    10141 gactattacg gcggcctgca ttgcggggag tgtttcgacg tgatgatcgg cggcaaatgg
    10201 aggcccaccc gcattgaaat ggccgaaaac tggtatcttg tcggcatccg ggccgacgac
    10261 ctttccggcc tgcgggtgcg gatttgacct atatctacct gcggcgtctg gtggttccgc
    10321 cttagacgcc gcttttgact ttctgacaag gagggatgcg attgcaggaa gaagttactc
    10381 aaaagaccat tgccctgtcc atgaaaacgg gcaagctcac ggctcaagtg ctgcaagcgg
    10441 cgctgaaaaa gtacctgcaa caccgggcca agggcaaaaa cacgctgcac cacggccagc
    10501 agagcctaaa gcagcttaaa aagcatgggg ctgatctctc caacattgag atcaccgagg
    10561 ccaacattgg agcgttcaag ccatgcgcca agaaatacgg cgtggatttt accttacgaa
    10621 aagataagac cacccagccg ccccactacg ttgtgatttt caaggccaag gatgcagata
    10681 accttgatct ggcgtttcgg gagtttaccg ccaagacgct ttccaaggag cagcggccct
    10741 ctatccgcaa ggttttgtcc gctgtgaagc aaaaggcggc ggcccagacc aagcagcggg
    10801 ccaaggagaa aatcaaggaa agggggctgg aactgtgaag cctgacgtaa agaaattgct
    10861 gatcctgaac ctcccgtatc tgatttttgt gtacctgttt gcgaaatgcg gggaggcgta
    10921 ccggctggcc gctggtgctg acctgtcggg gaagctcctg cattttgcgg atggttttgc
    10981 ggcggctttt gccaacccgc tgccaagcct gcacctgttt gacctgtgta tcggtatcgc
    11041 cggtgccgtg atcgtgcggc tggtggtgta ctgcaagagc aagaacgtga agaaataccg
    11101 caaaggcatg gagtatggca gcgcccgctg ggggacggcc aaagacatag cgccttacat
    11161 cgaccccaag tttgaaaata atatcctgct cacccaaacg gaacggctta ccatgaccgg
    11221 caggcccaaa gaccccaaga cagcgcggaa taaaaacgtg ctggtgatcg gtggcagcgg
    11281 ttcagggaaa acgagatttt tcgtgaaacc caacctgatg caatgctttc cgaccacgga
    11341 ctatccgact tcgtttgtgg tgacagatcc gaaaggcact ttggttctgg aaaccggcag
    11401 gatgttccag caggcgggct accgcattaa aatcctgaat accattaact tttccaagtc
    11461 catgaagtac aacccctttg tctacatcca ttcggaaaag gacgtgctaa agctggtaaa
    11521 taccctgatt tccaacacca agggcgaggg ggaaaagtca gcggaggatt tttgggtgaa
    11581 gtcggaacgg ttgttttatt cggccctgat cggctatatc tggtacgagg ccccagcgga
    11641 ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc agtgaagccc gcgaggacga
    11701 cccggagttt caaagccccg tcgatctcat gtttgagcgt ttggagcaga aagaccccga
    11761 ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc gcaggcaaga cccgttcttc
    11821 catcctgatt tcatgcggtg ccagacttgc gccctttgac atccgggagg tacgcgagct
    11881 gatggaggac gacgaaatgg agctggacac catcggggac gaaaagacgg tgctgtatct
    11941 cattatgagc gatacggaca cgacctttaa tttcattctg gctatgctcc aaagccagct
    12001 aatcaacctg ctttgtgacc gggctgatga tgtgtacggc gggcggctgc ctgtccatgt
    12061 acgcctgatt ttggatgaat ttgccaacat tggccaaata cccaatttcg ataaactcat
    12121 tgccaccatt cgaagccgtg aaatatcggc ctctattatt ttgcagagcc agtcgcagct
    12181 aaaagcgatt tacaaggacg ccgccgagat catttcggat aactgcgata gtgtgctttt
    12241 ccttagtggg cgcggcaaaa atgccaagga gatttccgat gcgctgggga aagaaacgat
    12301 tgacagtttc aacaccagcg agaaccgggg aagccaaacg tcccacggat tgaactatca
    12361 aaaattagga aaggagttga tgtcccaaga cgaaattgcc gtgatggacg gcggcaagtg
    12421 tatcttgcag ctacggggtg tgcgcccgtt tttatcggag aaattcgata ttaccaagca
    12481 tccccggtac aaataccttg ccgacgccga caagaagaat acctttgacg tggacagatt
    12541 tttgactatc acgcgccgga aacggcagca agtggtaacg caggatgaag tattcgacct
    12601 gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa taaacagcgg cttttttcat
    12661 tccaacaaca aaatcaaaaa atcattatga gccggaaacc ggcagaaaga ggtaaattta
    12721 tggcattttt taattccgca gttactactt tgcagactat cgtgatcgga ctgggcggcg
    12781 ctctgtgcgt atggggcggc atcaatctgt tggagggata cgggcaggac aatccggggg
    12841 caaaatcgca gggtgtcaag cagcttgtcg ctggcggcgg cgtggccctg atcggtgtca
    12901 ctctggttcc cctgctgtcc gggctgctgg gctgatcctg gcctttgtcc ctgtccaaac
    12961 ccttaactaa tcgcctccgc gtcctcccgg ccagaccggg agggcgcggg aaaggaggtg
    13021 ctacccgtga ttagtgaaat cattgaagaa tggataaaag gcatcctcat ggacggtatc
    13081 acgggcaacc tgtccgggct gttcgatacg gtaaatgcca aagtcggaga aatcgccgcc
    13141 gacgtgggac gaaccccgca ggcatggaac agcggtattt tcaatatgct gcacagcctg
    13201 tcggaaaccg tgatcgtccc catagccggg gccatactcg cccttgtcat gtgctatgag
    13261 cttatccaga tgataacgga aaagaacaat atgcacgact ttgacagctc catgttcttc
    13321 cgctggatat tcaaatccgc gtttgcaatc ctgatcgtaa cgaacacatg gaacatcgtc
    13381 atgggcgtat tcgacgccac ccaaagcgtc gtcaatcaaa gcgccggtgt gattatcggg
    13441 cagacatcca ttgattttaa caccctgata ccagaccttg aaagccaatt ggaggctatg
    13501 agcgtgggcg ctcttttggg gctgtggttc caaacccttg ttgtgggcct caccatgaat
    13561 atcctttcca tctgcatttt ccttgtgacc tatggccgca tggtggaaat ctatgttgtc
    13621 acgtcgctgg ggcctatccc ccttgccaca atgggcaacg cggaatggcg cggcatgggc
    13681 caagggtatc tgaaatccct gctggcgctg ggcttccaag cctttttaat catggttgtg
    13741 gtgggtatct acgcggtgct gatacagaat atcggttccg cttccgacat atccggcgca
    13801 atctggggct gtatgggcta tacggtgctg ctgtgcttct gcctgttcaa gaccggcagt
    13861 atatcaaagg ccgtattcgg ggcgcattga tcccaaaatc caagcataca caggaggaac
    13921 ttgcttattt caacgtacaa catcatttac gctgacccgc cgtggcggta tgactgcaaa
    13981 aacgtacagg gcgcagcgga aaatcactat tccaccatga gcattgacga gctgtgcgcc
    14041 ctgccggtgg aacggctggc gtccaaagac tgcctgctgt ttttgtgggc cacattcccc
    14101 atgctgccgg aagctctgcg gttaatcaaa gcgtggggct tttcattcaa gaccgtggct
    14161 tttgtatggc tgaaacagaa ccgtaaaagc cttacatggt tttacgggct gggccgctgg
    14221 acaaggggaa acgcggaaat ctgcctgctg gcaacacacg gcaagccgaa gcgtcgttct
    14281 gcctctgtcc atcagtttat tatcagcccg attgaacaac acagcaaaaa gccggatgtg
    14341 acccgtgaaa aaatcgtaga gctggcgggc gatttgcccc gcgtggagct gtttgccaga
    14401 cagaaaaccc ccggctggga cgtgtggggg aacgaggtgg aatgtgacct tatcctgacg
    14461 tgaaaggagg tgccaaatgg cttatgtaac cgtccccaag gacttaaccc atgtgaaaag
    14521 caaggttgtg tttgggctta ccaaacggca actgatctgt ttttccgggg cgctggtgat
    14581 cggcgtcccc ttatattttc tcacccgtga ctatcttacc aacagcgcgg cggccctgct
    14641 catggtattt gccatgctgc cgggcttttt gcttgccatg tacgagcgtc acggccagcc
    14701 ccttgaagtg gtgattatgc agatcatcca gtgctgcttc atccagccaa aggagcgtcc
    14761 ctatcagacc gacaacacct acaccgccct tgtgcggcaa taccagatgg aacaggaggt
    14821 aaaggccatt gtccaaaaag gaaatcccaa gagaaaccga aaaaacaaag ctgacccgcg
    14881 cccagcgaaa agaaattgac gcggtaatcc gtaaatataa gggcgacggc aggccccaca
    14941 cggcgcagca gagcatcccc tacgaggtca tgtacccgga cggcgtgtgc cgggtatcgc
    15001 ccggcgtctt ttccaagtgc attgagtttg cggacatcag ctatcagctt gcccagccgg
    15061 acacgcagac ggctatcttt gaaaaactgt gcgacctgta taactatgtg gacgcctcca
    15121 ttcatatcca gttttctttc ctcaatcgga aagttgaccc ggtgcagtac gccaagagct
    15181 ttgagatcgc gccgcagggg gacgacttcg acgacatccg ggccgagtac accggcattc
    15241 tgcaaaagca gcttgcaaac ggcaacaacg gcatggtaaa gaccaaatac ctgacattca
    15301 ccatcgaggc cgaaagcgtg aaagcggcgc gagccagatt gaagcgcatc ggctttgacc
    15361 tgttaggcta tttcaagagc atgggggccg tagcccatgt gatggacggc tgggaacggc
    15421 tgaacctgct gcatggcgtg taccacccgg acggcgagat attcaatttt gactggaagt
    15481 ggctggcccc gtccggcctc tccaccaagg attttattgc cccgtcctcc ctgtgcttcg
    15541 gcaatgcaaa gacattcggc atgggcggca aatacggcgc ggtaagtttc ctgcaaatcc
    15601 tgtccccgga gctgtcggac gatatgctgg ccgacttcct caatacggaa agcggtgttt
    15661 tagtcaatct ccatgtgcag gccattgaac agaccaaggc gatcaagacc atcaagcgca
    15721 aaattaccga ccttgacgct atgaagattg cggagcagaa aaaggccgtc cgcagcggtt
    15781 acgatatgga cattcttccc agcgaccttg ccacctacgg cgaggacgcc aagaagctgc
    15841 tgacaaagtt gcagacccgc aatgaacggc tgttccagtt gacgttcctt gtcctcaacg
    15901 tggccgacac caagcaaaag ctgaataacg acgtgttcca agccgcaggc gtggcccaaa
    15961 agcataactg tccccttgtc cggctggact accagcagga acagggcctt gcaagcagcc
    16021 tgccgctggg cgtcaatcag atcaagatac agcgcagcct tacgacttcc agcgtggccg
    16081 tgttcgtccc ctttgtgacg caggagcttt ttcagggcgg cgcggccatg tattacggca
    16141 tcaacgccaa gtcccgcaac atgattatgc tggacaggaa gcaggcccgt tgccccaatg
    16201 ccttgaagct cggcaccccc ggcagcggca aatccatgag ctgtaaatcc gagatcgtca
    16261 gtgtgttctt aacgacgcct gacgatattt ttatttcaga cccggaggcc gaatattacc
    16321 cccttgtcaa acggctccac ggacaggtaa tccggctgtc gcctaccagc aaggactttg
    16381 tgaacccgct ggacatcaac ctgaattatt cggaggacga taacccgctg gccctgaaat
    16441 ccgactttgt gctgtcgttc tgtgagcttg tcatgggcgg caagaacggg ctggaagcca
    16501 ttgaaaagac cgtgatagac cgggctgtgc gcgtgatcta ccggccctat ctggcagacc
    16561 cccgcccgga gaatatgccg atcctgtccg acctccacaa agcgttgtta gatcagcatg
    16621 tcccggaggc tgaccgggtg gcgcaggccc ttgacctgta tgtgtccggc tccctgaacg
    16681 tatttaacca ccgtaccaac gtggacatcg gcaaccggct tgtgtccttt gacattaagg
    16741 agctgggcaa gcagctcaaa aagctgggga tgctcattgt ccaagaccag atatgggggc
    16801 gcgtcacggc caaccgaagc caaggaaagg ccacatggta ttttgccgac gagttccatt
    16861 tgcttttgaa agaggaacag acggcggcct attctgcgga gatttggaag cggtttagga
    16921 aatggggcgg cattccaaca ggtgccaccc agaacgtcaa agacctgctt tcctccccgg
    16981 aaattgaaaa tatccttgaa aacagcgact tcattacgct gctcaatcag gcgtccgggg
    17041 atagaaaaat ccttgcggaa cgcttgaatt tgtccaccga acagcagaag tacatcgaca
    17101 attccgaacc cggcgagggc cttttgatct ttgaaaacgt ggtgctgccc tttaccaacc
    17161 ccatcccgca caacacccag ctttacaaga ttatgacgac ccgcttaaac gaggtggcgg
    17221 gagtatgacc cggcgacgcc acaaaggagg tgaaccacca tgaaagagct gaaagccaag
    17281 gcgaaagtga cgcaggccat gacccgcgac gggctgacgc tggaaaatca ggccaccggc
    17341 gaggtggaga acatttccag ccgggaggcc gaacaggatt tgtccgcgcc ggggccgggc
    17401 ggcaccgccg ataagctgct ggaacgggcc gacgccatcc atgacggcca taaggccaag
    17461 aaagccgcga agcaggccgg ggctgtggcc gccgaggggg acgctgccct gcaccggcca
    17521 tccgcccggc tccaactgtc ggaggaagaa cgggccgacc cggatttaca gccctatatc
    17581 cgaaaatcgg acaaacgggc cgataagctg gataaggccc gcgccgccat ccccaagaag
    17641 cgcgtcccgg tcaaggaaac cgtctttgac gaggcaagcg gcaaagcaaa gaccgtcctg
    17701 cactttgaca aacaggacaa ggggcctccc tccctgaaac ccaatccggc aggccgtcct
    17761 gtttccgagg tttttctatt cggccacggg aaaatccatg aagtcgagca tgaaaacgtg
    17821 ggtgtggagg gcggccataa gggggaggaa cttgccgagc ggcaggccgg aaaagccatc
    17881 cgcagcacca tccggcacca caggctgaaa ccctactggg acgcggaaaa ggccgagcgc
    17941 aggctattgt ctgccaacgc ggagtatttc taccagaaat ctttgcggga taacccggag
    18001 atcgcacagg cggccagcaa ccccatttcc cggttctggc agaaacagca gatcaagcgc
    18061 cagtacgcca aggccgcccg gacagcgggc cagaccgcag gcgcggcggc acagggcgcg
    18121 gccaagacag ccggggcctc cacgaaagcg gccaaggccg cagccaaaga aagtgaacgg
    18181 gcggcctcct ttgtggcccg ccactggaaa ggggcgctgg ccgtccttgt gttggggctt
    18241 ttgatgctgc ttatcatcgg cggcttacag tcctgcaccg ccatgttcgg cagcgccgga
    18301 agcggcatca cggcgtcctc ttacttttcc gaggatagcg atatgttggc cgccgaggac
    18361 gcctacgcgg agctggaagc cgagctgcaa gactatctgg acaactacga ggccacccat
    18421 gactatgacg aataccattt tgaccttgac gagatcagcc atgaccccta tgtgctgatt
    18481 tccattctgt cggcgctcca tgacgggata tttaccatcg gggaggtaca gggtgatttg
    18541 tccatgctct ttgacaagca atatatcctg acagaaaacg tcgtggtaga aacccgttac
    18601 cgcaccgaaa ccgatacatg gacggacgcg gagggcaaca cccatacaga cacctaccaa
    18661 gtgccgtatg actattacat ctgctacgtc acgctggaaa acttcgacct gtcccatgtg
    18721 ccagtctata tcatggacga ggaaacgcta agtttctatt ccctgtatat gtccacgcta
    18781 ggaaaccggc ctgatctgtt ttccggcagc cagtacccca acgcctccac cctgacgccg
    18841 ccgacctact acgacgtacc cccggaggct ttggaggatg ccacctttgc cgccatgctc
    18901 aaagaggcgg aaaaatatat cggttatccc tatgtgtggg gcggcagctc tcccagcacc
    18961 agctttgatt gcagcggctt catttcgtgg gtaatcaatc atagtggatg gaacgtggga
    19021 cggctggggg cgcagggcct atataacatc tgcacccctg tttccagttc gcaggccaag
    19081 cccggcgacc ttgtattttt caagggaacc tacgacaccc ccggcgtgtc ccacgtcggc
    19141 ctgtatgtgg gcaacaatgt gatgctgcat tgcgggaacc cgatttccta cacgaacctt
    19201 acttcaaact actggcaatc ccatttttac agttacgggc gtttacctta acgccagaaa
    19261 ggagctttca attatggcaa ccaaacttga ccgcatcgaa aaggacatcc agaaaaccaa
    19321 gtctaagatc gcggagtttc agaaacagct tcgggagctg gaaacgcaga aaaccgaaca
    19381 ggagaatttg cagatcatcc agcttgtgcg cggcatgaac atgaagccgg aggaatttgc
    19441 cgcgttctta cgcagcggcg caatgcaggc cgcccctgcc gcaacccctt accatgaaca
    19501 ggaggacaat gcccatgaag aataagacca gacggatttc ccgtattctc cccgcacttc
    19561 tggccgtgat gctctgcgtc acggcctttc ccgttaccgc ccttgcaggc ggcaatgacc
    19621 cggcaccggc cccgctgccg gaggctacaa ccgaacccgc tacgggcggt ttggagcctg
    19681 aaacagacga aaccggcacc gccacggacg ggacagaaac cgaggccgaa aaagataccg
    19741 gccctctgac cggcaaggac atcaccgacc tgttttccgc gctgttcggt tccaaggtga
    19801 gcattgccgc cacggacgac ggcatccaga tcaccaccgg caccgacaaa aaagaacctg
    19861 aacagaccgg caccgtcacc accaacggcg gcaggctgaa tgtccgcacc ggcgcggggc
    19921 ttgacaagac cgcgttcacc cagcttccca atgggacgac ggttgaggta gtcggcacag
    19981 acggcgactg gattaaaatt ctcttgccgg agcgcatcgg ctatgtccat tccgactata
    20041 tgaccgtcag cgaaaaggaa gtagcggcta ccggcgaggg cggtttttcc ctctccattg
    20101 acccggagga aattgcttct ctgctggaac tgttcaatgg cggcggggcc agcgccgccc
    20161 tgtccccgga cgggaacctg tcactgattg acgacatcgg cagcgccgcg aaaaatggca
    20221 agcagtttat caccgtggaa accaaaaacg gcaatgtgtt ttatctgatt attgaccggg
    20281 acgacgaggg aaaagaaact gtccatttct taaatcaggt ggatgaagcc gatctgctga
    20341 cgctgatgga ggacggggaa accgtcaccc ccgctgctgt ctgctcctgt accacgaaat
    20401 gcaaggccgg ggctgtgaac acgaattgcc cggtatgtaa aaccaacctg accgagtgca
    20461 gcggcccgga gccgcaggaa ccccagccgg aggaacccga agccccggag gaagaaccca
    20521 agggcggcgc gggcggcctg atcgtgttcc tgctggtggc gctggcgggc ggcggtgcgg
    20581 ccctttacta tttcaagttc aaaaagccca aggctgaaac caccggcagc gacgatttgg
    20641 gcgagtatga ttttggggag gacgaggatt tggaggacga ggaacccgaa accgagtttg
    20701 acccggaagc tgattttgtg agtgaacagg aggacgagga atgacatata aacttgtaat
    20761 cgcggaaaaa cccagcgtgg cccgcagcat tgcgggcgtg atcggggcca cggagaaaca
    20821 cgacggctat ttggagggga acggctatct ggtgagctgg tgcatcggac atctggtgtc
    20881 ctttgccgac gccggacgct atgacgagcg tttcaaaaaa tggcgctatg aggatttgcc
    20941 cattctcccg gaaacatggc agtacatcgt ccccgacgac aagaaacagc agtttgacgt
    21001 tctctgttcg ctgatgcagc gccccgacgt gaccggcctt gtgtgcgcca ccgacgccgg
    21061 gcgcgagggg gaattgattt tccgtttcgt ctaccagatg gcgggatgcc aaaagccctt
    21121 tgagcgcctt tggatttcct ctatggagga cagtgctatt aaggacggct ttgcccacct
    21181 gaaaccgggc ggcgactatg atccgctgta tcaatcggcc ctgtgccggg ccaaggccga
    21241 ctggctgatc ggcattaacg ccacccggct tttttccgtc ctgtaccaca agaacctgac
    21301 tgtgggccgc gtccagacgc cgaccttaaa aatgctggtg gacagggacg ccaagattac
    21361 cgatttcaag aaagagaaat atcacatcgt ccatatcacg gcgggcggcg cggacgcggt
    21421 aagcagccgc ttttccgacg ctgccgaggc aaacaccgtc aaggcggctt gtgcaaaagc
    21481 gcaggccgtt tgcgtttccg tgacgcggga gaaaaagacg gaacagccgc ccaagctcta
    21541 tgaccttacc accttgcagc gggaggctaa ccggcttttc ggctataccg ccaagcagac
    21601 ccttgactat gcccaggcac tgtatctaaa gaagctgctg acctatccga gaacggatag
    21661 tcaatatctt accgacgata tgctgcccgc cgtggaaagc cttgtgtccg gcctgtggga
    21721 gctggtgccg tttgccaagg gcctgaacct ttccccgcag tttgaccgca tcctgaacag
    21781 caaaaaggtg tccgaccacc acgccattat ccccaccgct gaatttgtga agcagggctt
    21841 tggcactctg gccgagagtg aaaaaaagct gctgtccctg atctgctgca agctcttatg
    21901 tgccgtggcc gggccgcata tctatgaggc cgtcaccgcc acatttacct gtgccgggca
    21961 ggagtttacc gccaagggca agaccgtcct ttcccccggc tggaaagaga ttgaacgccg
    22021 gttccgttct tccttaaaga ccgacgccga cgaggacgcc gaggcggtgc gggagctgcc
    22081 ggagctttgc gagggccaga cctttgcaga cgtggccgcc tctgtcaccg agcatttcac
    22141 cacgccgccc aagccctaca ccgaagatac gcttttgtcc gctatggaac gggccggggc
    22201 cgaggatatg ccggaggatg ccgagcgcaa gggattaggc acaccggcca cccgcgccgc
    22261 cattctggaa aagctggtgc agatgggctt tgtccagaga aaaggcaagc agctcattcc
    22321 caccaaggac ggcatcaatc tggctgtggt gctgccggag gcgcttactt ctccccagct
    22381 tactgccgaa tgggaaaacc ggctgaccga gatcgccaag gggcaggccg atcctgacga
    22441 atttatggac ggcatcgaga cgcaggcgcg ggagctggtg cagacctatt cctgtatcag
    22501 tgaggaaaag cagaaattgt ttcaggccga gcgtgttcct atcgggacgt gtccccgctg
    22561 tggtgaggct gtctatgagg gcaagaaaaa ctactactgc ggaaaccggg cctgccagtt
    22621 cgtcatgtgg aagaatgacc gattttttga ggaacgcaag aaagcgttca ccccgaagat
    22681 cgccgctgcg ctgctcaaaa ccggcaaagt caaggtaaag ggcctctatt ccgtaaagac
    22741 cggcaaaacc tacgacggga cggtgctttt ggctgatacc gggggcaagt acgtcaacta
    22801 ccgcattgaa cacaggagct aaaggacagt aacacagcaa gcatagggag tgtggctaca
    22861 ctcccggtaa aatcccccgg ccgcgtccct gccgggccgg ggatttttgc ttgttgggga
    22921 agtccccaac cccctatttt ttgaaacaga acagagaaag gagtgaccgc gtgaacgaag
    22981 tattttctat ccaagtccat aaccggcagt ttttggaaca gggcaaacaa ggcgtctggc
    23041 tggatttgcc caccacaacc gaaacgctgc aagccgccct gcgggatgtt ggcattactg
    23101 ccgacaatcc gcaggatttt tttatcaacg ggtattcctg cccggaggac agacaccttg
    23161 ccattcccta tgacatggtg ctggccgccg acgtggacga gctgaatttc cttgccgccc
    23221 ggctgggcca gcttgacgcc gccgagatcg cagagctgaa cgccgccctg caaaatcccc
    23281 gtggcggctt tgagaatatc ggccagatca tcgactacac cgaaaatgtg gattactatg
    23341 tgcatcttcc cgacgttagg ggggatggac agttgggcga ctactacctg aaccgttccg
    23401 gcatggtgga tatgccggag gaatggaaag ccgggattga tacggcgcag tttggacgcc
    23461 acattgccca gcaggaacaa ggcgttttta cccagtacgg ctatctggtg agaagcggcg
    23521 acgaatggca gcgcgtccat gagggccagc cggtgccgga ggaataccgg gtgatgcact
    23581 acccccagcc ggaagctgac cgggaggaac cgcaggccca gcagccggag gcatccgaaa
    23641 gccccgacaa agcgccgccc ccggttatcc cgatcatgct ggacggcatg aacagcaaag
    23701 aacgcatgaa agtgattact gaccgtctgg aaaccggcgt acaggagctt tttgaaagta
    23761 gccgctatat ggcttactta tccgctatgt ccaagatgca cgattacagt ctgaacaata
    23821 cgctgctgat cgccatgcag ggcggttcga ttgtggcggg ctataacagg tggcaggatg
    23881 aatttgaccg ccatgtgaag cggggcgaga aaggcattaa gatactggcc ccggccccgt
    23941 tcaaggttaa gcagcagatg gagaaaatcg acccggccac ccagcagccc gtgatcggcg
    24001 cggacggcaa gcccgtgacc gaagaacagg aagtgacggt tccggccttt aaggtggtga
    24061 cagtctttga catcagccag accgagggca aggattttcc catgccctat atcagtgaac
    24121 ttaccggcga cgtgcagcag tatcaggatt tctttgccgc gctggaacgg acatcaccct
    24181 atcccataag cattgagccg atttcagacg gggccaaggg ccgctgcttc tacgcaaaac
    24241 agcgcattgc catcaacgag ggcatgagcg aattgcagac cctaaagacc gccatccatg
    24301 agattgccca tgccaagctc cacgccattg accgggaggc ccccgccgag gaacaggcgg
    24361 cccggccaga ccgcagcacc cgcgaggtac aggccgaaag cgtggcctat accgtctgcc
    24421 agtattacgg gctggacacg tcggactatt cttttggcta tgtggccgat tggagcagcg
    24481 gcagggagtt atccgagctg aaagcctccc ttgaaaccat ccgggccacg gccagcgaat
    24541 tgataaccga gatcgacggc cattttgccc agcttcagca ggagcgcgag gcccggcagg
    24601 aacaggaggc cgtcacggtt gacgttaccc cggaggcagc ggccccggcg caggccgagg
    24661 aagaacaggc cccggccctt gatccggcag ccgagccgct tgtgactgtg ctatggagcg
    24721 aaagccccca gctaaaggac ggccagcaaa tgccgttgca tgaggccgac gcgctgtttg
    24781 cccgtctgga tgcagcatat ccggcagagg caggctatga caaaaccgcg ttccgcattg
    24841 actgtatctt tcagggcgag ccggacagct acgaggggcg gcaggatttt ggagataggg
    24901 acggttccct gatcgaacat atccagagct accaccaatt ttacgcgcag gacgaaaact
    24961 ggaaaaagca tgtaatcgct acaaccggca tagagggctg ggagcaggac aaggcccatc
    25021 gggatatgct gttaggcgag tttgtcccct atatgaagct gcattgcagc cttgcccaaa
    25081 tggaacagac ggcgcagggc cgcttacagt ccggggaaac cctgacgccg gaggaaacgg
    25141 cctatttggg ggcgcttctc tcttatgtgc aggagtgccg ccccatgctc aatcagggcc
    25201 agtatcagtt gccggagccg ccgaaactgg ctgacttcga ccagagcttg caggactaca
    25261 aagcacaggt acaggccgag atcgcgcagg aggccgccgc agccggtatg accgtggagg
    25321 aatacgcggc ggctggctat gaagcacccc aacaggacac cttttccatc taccagcttc
    25381 gaggcgggga ggaaacccgc gactatcggt ttgagccata cgagcgttta caggccgcag
    25441 ggctggccgt ggacagtgcc aactatgacc ttgtttatac ggcaccgctg gataaaagca
    25501 cgtctttgga ggatatttac cgcacattca acatcgacca cccggcagat tttaggggac
    25561 acagcctttc cgtttccgac gtggtggtgc tgcaccagaa tggacaggac accgcccact
    25621 atgtagacag cttcggctat atggaagtgc cggagttttt gcgggaaaat cccctgcgta
    25681 ccgccgaact gtctacggag cagaacgaaa acatgattga cggcattatc aacaatacgc
    25741 cgcccgaacc gcagccggag gaacccgaac agctatcttt tgaggatgtg gcagttgctt
    25801 ccggccagac acaggaggaa cccccggagg ctgcgccccc gcaaacggct gtccgctatt
    25861 atcccatcaa tgagggagcc gcccgccgcg ccaaggaagc catgagcttt tctgactacc
    25921 ggcccggcag cgccacggcg gaataccggc actatgtcga tcaggccgtg gaaattgcgg
    25981 agcgccagaa aaagcgtgtg gagccagagt atcacgaaaa gatagacagc ctccttgaca
    26041 cctacgcccg caagctggcc gagaacatga ataagggcta tgagattacc gcccgcgtcc
    26101 cctctgtcct gattgccggg ccgtccaatt tcccggtacg ggcgaaagaa aagcagaacg
    26161 ccgccagtga ccgcaacatg gaggaatttc agtacattca gggcttgctt gacaaaatcc
    26221 gcagcaccgg catgggtggt atcagcgccg acgatccgca ggccgtttcc aagctggaaa
    26281 agaaactgga aaagctggaa gcgtcgcagg agttgatgaa ggccgtcaat gcctactacc
    26341 gcaagcatgg gacgctggac ggatgccccc atctgaccga aagaggcatt gaaaacctga
    26401 aagcggatat ggcgtcgggc tggcactatg agaaaaaacc gttccagtcc tggcagctct
    26461 ccaacaataa cgccgagata cgccggttaa agggccgcat tgaggaattg acccgccaaa
    26521 aggaggcggc ctatgtggga tgggagtttg acggcggcac tgtggagatc aaccgggtgg
    26581 ccaaccgcct gcaaatcttc tttgagggga agccggatgc tgctgtacgc gacgagctga
    26641 aaagcaacgg tttccgctgg tcgcccaagg ccggggcatg gcagcggcag ttaaacgaca
    26701 ccacaatccg cgtcgctgac cgtatcaagt gcatccagcc cctttccggc gaaaagccca
    26761 gcgccttaca gcttgcggcc cgcagggaga aagaaaagcc ctctatccgg gcgcagctca
    26821 acgcggccaa gaccgaacag gccaagcagc caccggcgcg ggaaaaatca aagggattgg
    26881 aggtagagta aatgcagaag tttgaaaatg tggacgttct ggccgcgctg gaacagatca
    26941 tgcggcagaa caccgcgttt tatcaaagtg actttggcat tgacaaagac attatcaggc
    27001 gggatgccgc cagcgatcag gccgcagaca agaccctgct ctggatgtcc cggccctccg
    27061 ggacgtactg tttccgggaa cgtgatgttt tcctgaaaga cacccgacag tacaacacat
    27121 ggaaatttta cggggagcag acccgtgaca agatactggc ctacgcggtg gagctgaccg
    27181 gcacacaggg cggcactatc cggggcaatc tctatgagct ggactatccg cagcatttcc
    27241 ggcacgtcat tgaaaccgcc cagccggtga gtgtgaaccg gctcttttat gagcatggca
    27301 cccgtgacat cccggagagc cagcgttttg acggaagccc tgaccgggta ttgggaaact
    27361 ttttgtacta tgaggcccag ccccatgacc cggctgtgct gcaggaggcg ctacggacgg
    27421 agcagcacag ccgggggcgg gccgtccccg gcgatttcaa agtccacgtc accgctttgc
    27481 atgacagccg gattgaaacc gaggcccggc gtattgtgga cagcctgaaa gatctgtccg
    27541 cgcctaacag ccccaataaa acgcatttca tggtggagat ctccccgcac tttgcggcga
    27601 ttgcttccac caaggataat gaccggctct ttgccatgct gccctataaa tccctgtgtt
    27661 ttaccgggat gaaaggcagg catggcgtgt atgccgtgat taaccgggac gagcgccggg
    27721 atgtgagtat tcgcaggccc cggccctcta tccgcaagca gctatccgac agcaaacagg
    27781 ccgccagccc aaaaaaggcg gcggccagaa ccaaaaagaa tgaattggag gtataaggaa
    27841 tgatgcactt taccgtggag gaagaaaacc tgatctgtat gtaccacaat gcagaccggc
    27901 gcagaacgat aaacaggctc accgccgtca tgctggttat ggatggggat atgcgggcgc
    27961 aggcccagca gaccctttcc aagctggaac gcatgacgga tgccgactat gacggccaga
    28021 agttccactt cacagacgaa taacgaacag cagacgccgg gcgcggcggc catgtggtcg
    28081 cttgcgtccg gcgtcttttc tttttgccta ttcctacgca tttagaaccc ggtattgtcg
    28141 gggataggat gaagtattcg cttgcccctg caaacatgcg ggagaagcct tgcagcgggc
    28201 ggttttcaat cccttaaatg cgtaggttgc agctataatt agacccctga aacagatata
    28261 gccgcgcagc gggtgtatct gtttcagggg tgtgcaggtg atagccgcaa agcggcgtaa
    28321 gagggtgcag ccctcttgcc gtctatctct gttccagcgg gaagctcggc aatgcttcca
    28381 gcagtttcag ggaaatgcgc tgccgtatat cttcgtcaat gactttcttc tttttgccgt
    28441 ttgcctgtct gatctccacc gtggaaagct cgttgatgta gtcagcataa tgggccaaca
    28501 ctttttccgt ggcccacctc tcaccggcaa cggcggcttt gatcgtttca tagtccagca
    28561 gcttttcttg tccgtccatc ctgatacccc cgtcagactg attttttcta tgataccata
    28621 ttcggaagca tggaaccaga gaaaacggcg cttttctgct ggtaaattct acgcatttag
    28681 aacagcactt cggcggggat aggataaaac acccacatac cccgctgacc atgccggaaa
    28741 agccttgaaa tataagggct tttacagctc taagtgcgta ggtttccagt atcttttccg
    28801 ctggcgctcg gcctctttgc ggcggcgcat ccgtttggag caggccgcgc agtatttggc
    28861 ccggttagac ttcggcgtga acgtggcccc gcactcacag cagcgtttca cttcgtcccg
    28921 gtctttggtg atctcggcat acagggccgc atccagcggg aggacagcgg ccttaaacca
    28981 gcggcacaga agcgaatagg aaatgctctg tacgcaaata cattcttcgc cgtcgtccag
    29041 caacaggcag ttgccgtcac aatagttgca gcactcatgc gtcagcttcc gggccttgcg
    29101 atattgaggg taattcattc gtggaatact catagctctt gcgtccattc ctttcccggc
    29161 gtcatacgca aaatgccgtc cacattctgc ttgatgatgc cgtattcctg catctgcttt
    29221 ttcgcttcgc cgtacttctc atacagccgt tccttttcct cggccaatcg cttgtactcg
    29281 gctttcaggg cgtacagatc ggggagcttt ttcacaccct gtccctttag ggcctttgcc
    29341 gcagcttcat acaaaatcaa cgtgctttca tgctcacgcc ggaattttgc cttatccgct
    29401 gcattgcggt attccatagc cacgggccag agctgcttgt aggtagaaat attctttatc
    29461 agcaccgcca tatccgcaag ccggtgttcc atagctttga gggtagcggc ggcctcgtcg
    29521 ctggcggcgg tgatctcggc cactttgctt tccaaatccg ggtaggtgtc tatcccatgt
    29581 tcagtcagga aattcaaagt ctttgcggcc tgcttcaaat tgtggagctt cgcccacctc
    29641 tcatagcctg ccgactgctg ggccttaatg ttgttttcca gatcaatccg caaagaaatc
    29701 cccttgcgct ctttgggcgc tctggtgata acgcggcctt tgatacgctc tttgatggct
    29761 tcctcggtgt aggcttcccc caatgtcttg cagcgggtaa agcgttcctg tccgggagcg
    29821 cggaaagaaa taaactttcc cggcttgatc tcatagcctt gcgcctgcat gagccgcagg
    29881 aaatcatcaa aatcttttgc ctgcgggata gcggcgtcaa tggctgcttt gagcttcgct
    29941 ttccagctcg tccctttccg ctgggcgtcc cactcggcat agctcttgcc cttactctgg
    30001 ccgggcttca ccacggacaa tccgttttcc ttgcacagcc tgtcgctggt gcgccggata
    30061 taggcatagc tctgtttgtt ggaaatgtac ttgcgctgat ttaccatatc cacggcacag
    30121 aaaataatgt gattgtggac gtggccctta tcaatatggg tagtcagcac atagggatat
    30181 ttcccttgca gcacttcctc ggcaagctgc ttcccgatct catgggcctg ttccggcgtg
    30241 gcttcgccgg gggcaaagga ttgtatcagg tggtggccca gattattgcc cttttggtat
    30301 gcctgcgcca gcagcatttc aaattcaatg tccgctgttt cataagagca ggcaaaggag
    30361 gccaccagca gcttgccgtc agttttgtcc gggttctcga tgtagtcaag ggcttttttc
    30421 agcgttgact tgataggatg tatctttgta actgccatat ctgcgccagc gcccccttta
    30481 tatcctcgat gtcctgtgcg tacaccggcc ccataccgtt cagcctcttt gcaatctgat
    30541 tgacgttgac gccgatcttc tgtatctcgg cggtctgcgc ccggatgtcg ctggtgtcca
    30601 tgttgacgat atagccgtca atgagcatct tccgggcgta ggctgaaaag ttcttcgtat
    30661 gcagcagcgc cattttctgt tcaatcatag cccgttcctc cggcgtcaca gggacgcgca
    30721 gcacgatttt tcgctttctg tttgccatgc ttcaccgtcc tttccattca ggggtttggg
    30781 gatttcccca acaagcaaaa cggacgcggc ccggcaggga cggggcaggc cgtttttccg
    30841 ggagtggcta cactccctat gcttgctacg ttactgtttc tccactaaca agacgtttcc
    30901 aaagccccaa actacccgca aaatagccct ctggaaagaa aaaagtgcaa aaaaatagaa
    30961 gctacgctgt gcagctcccg ctaaaagtaa aacagaggat gccagttaca catcctccgt
    31021 ttcattcttc ttcggctttt caattagtat ggcgctgtca atcgcgccct cgatgatcgg
    31081 caggtattct tcggggcaga gtttcagctt gtggctgacc ctttgccgca gttcgctttc
    31141 ctcccgcatg atctccgggt taaagtagcg ttccacagga agcccgcagg ctttaataag
    31201 ctgtatgaca accggcagac tgggaatggc cccgtcgttt tcgatgttgg caagatagcg
    31261 ccagtcaata tttattttct ctgccattgc tttgcgcgaa acgcgctttg cttgccgggc
    31321 cgctttcaca tccgcgccga aagtttcaaa gccgggacaa tcttcaatat tcgccatatc
    31381 gcacctccca cttacattgt atatttcata ctctgcccgt ggaatgttgg tatatgcacc
    31441 tttcttaaag tttatatttc acattttgtt tcttttattc ttcgagcaaa agaattataa
    31501 tgtaccctgt ggaggtgtac tatggactac atgacgttga aagaggcagg cgagaaatgg
    31561 ggtgtgcccc cacgatggat aaactactat tgtgctgcgg gacgcatccc cggcgctgta
    31621 aagatggcga cgatctggct cattcccaaa actgcggaaa agccgattga caggcgtaca
    31681 aagaaaggaa aggcggttac tgaatagtat aatgaaaccg gcgcattttg gatatggttt
    31741 tgaagagaga gaaagcatgg tattttgtca cggaaaaact ataagatgat agatggcagg
    31801 ctgctccaga ccgataaaaa gtattctcat ctgaaacaga aagaaaagat ttacacatgg
    31861 atgtatgaag aagcgaaact ttatcatgac agaaatggaa aatgcccgaa acaaagacga
    31921 ggaagctgct gctgtatcag ccgtatatgg tagaattgaa aaggccggca tctggattct
    31981 ttatggtgaa gtctccagac attggataac tgctggtcta atgcgccttt gagtatgggg
    32041 tgattgattt taataccggg gaggtacggc tttttgagga aaatattctg ggatttacat
    32101 ttggcgatta ttacatttcc tgtaactgca aggacacgga tgatgaagat atgaggaata
    32161 tagacttgct gattttctac tgtcttgaac ggtattagga ctgaatctgc tgcgcggagt
    32221 gcggcaagag cgaccggagg gagtacacac aggccctgcc tgtgtgaaaa gccataccgc
    32281 aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag gcttggggtg tccccaacaa
    32341 gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag aatatccgta aaggatattc
    32401 tgatccattg cttgccagta ttatcgggat gttttgacaa ccgctgtaat cgaaacatcc
    32461 caaacattaa gaaggtgtta attattatta tcttgattta attcgtttaa accaataata
    32521 tagatacata caccatgaaa agaagcgagt atcccaatga cggatgatta aaatccgaaa
    32581 agaaatcgct ttgaaactac agggaaacta cagactgtta tgttatcttc ttaaaatgga
    32641 gggattttta tgtcgatacg aattctactt gtcgaggatg atgatcatat ctgtaataca
    32701 gtaagggcgt ttctggctga ggcaggatat caggtggatg cctgcacaga tggaaatgag
    32761 gcatacacca agttttacga aaacacttat caactggtta ttcttgatat tatgctgccc
    32821 ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga aaaatgatac tcccattctg
    32881 atgatgacag ccctgtcgga tgacgaaaac caaatccggg cgtttgatgc agaggcagac
    32941 gactatgtaa caaagccatt caagatgcag attttactaa agcgggtgga agccctgctg
    33001 cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca ggctgacact tctgccggag
    33061 gatttcacgg tactttgtga cggtacagag ctgcccctga cacgaaaaga atttgaaatc
    33121 cttttgctgc tggtgcagaa caaaggcaga accttaacgc atgaaatcat tttgtcacgt
    33181 atatggggat atgactttga gggtgatggc agcacagtcc acactcatat caaaaatttg
    33241 cgggccaagc tgccggaaaa tatcatcaaa accattcgcg gtgtaggata ccgattggag
    33301 gaatcataat aatggaaaga aaagggattt tcattaaggt tttttcctat acgatcattg
    33361 ccctgctact gcttgtcggt gtaacagcaa cactgtttgc acagcaattt gtgtcttatt
    33421 tcagagtgat ggaattacag caaacagtaa aatcctatca gccattggtg gaactgattc
    33481 agaatagcga taggcttgat attcaagagg tggcagggct gtttcactac aataaccaat
    33541 cctttgagtt ttatattgaa gataaagagg gaagcgtact ctatgccaca ccaaatgcca
    33601 atacatcaaa tagttttaga cccgactttc tttatgtggt acatagagat gataatattt
    33661 cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta tcaagggctg acaattcggg
    33721 gaattgttat gattgcgata atggttgtat tcagcctttt atgcgcgtat atctttgcgc
    33781 ggcaaatgac aacgccgatc aaagccttag cggacagtgc gaataaaatg gcaaacctga
    33841 aagatgtacc gccgccgctg gagcgaaagg atgagcttgg cgcactggct catgatatgc
    33901 attccatgta tgtcaggctg aaagaaacca tcgcaaggct ggaggatgaa atcgcaaggg
    33961 aacatgagtt ggaggaaaca cagcgatatt tctttgcggc agcttctcat gagctaaaaa
    34021 cgcccatcgc ggctacaagc gttctgttgg agggaatgct tgaaaatatc ggtgactaca
    34081 aagatcattc taagtatctg cgcgaatgca tcaaaatgat ggataggcag ggcaaaatca
    34141 tttccgaaat actggagctt gtcagcctga atgatgggag aatcgtaccc atagctgaac
    34201 cgttggacat agggcgcacg gttgccgagt tgctgcccga ttttcaaacc ttggcagagg
    34261 caaacaacca gcggttcgtc acagatattc cagccgggca aattgtcctg tccgatccga
    34321 ggctgctcca aaaggcacta tccaatgtca tattgaatgc agttcagaac acgccgcagg
    34381 gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa atgccgcctt tttgttttga
    34441 acatgggcgt tcacattgat gatactgcgc ttccaaggct gttcacccca ttctatcgca
    34501 ttgatcaggc gcaaagcaga aaaagtgggc gaagcggttt aggacttgcc atcgtacaaa
    34561 aaacgctgga tgccatgagc ctccagtatg cgctggaaaa cacctcggat ggcgttttgt
    34621 tctggctgga tttaccgctc acatcaacat tgtaaatatt taaaacttaa atgattttga
    34681 ccgacaggta taaccctgcc ggtctttttt tgctgctaca ggaaaactac agactggcta
    34741 cagggaaagt acagatgcgc ttgccataat aacaatcgta ccagccacaa atcgtagttt
    34801 tattgcaaag gaggcattcg atcaaatgga aaaaagcaac tatcattcca atgcggatca
    34861 tcacaaacgg catatgaaac aatccgtgga aaagcgggct tttctatgtg cgttcattat
    34921 ttcgttcaca gtctgcacgc tgtttttggg gcggagactg gcttccgtat tggaggcaac
    34981 acagataccg cccatccctg caactcatac aggcagcagc actgacgtag tggagaattt
    35041 ggaggaaaac gctcttgcca ccgccaaaga acagggagat gaacaggaat ggagcctgat
    35101 tttagtgaac aggcagaacc ccatccccgc acagtacgat gtggagcttg agcaactatc
    35161 aaatggtgag cggatagata ttcggatttc tccctatctt caagatttgt ttgatgccgc
    35221 aagaactgat ggagtttacc cgattgtcgc atccggatac cgaacaacag aaaaacagca
    35281 agaaattatg gatgaaaaaa ttgccgaata taaggcgaaa ggctacacct ctgcacaggc
    35341 taaagcggaa gcagaaactt gggtggccgt gccgggaacg agcgagcatc agcttggtct
    35401 tgctgtggat atcaatgcgg acggaattca ttcaacaggc aacgaggttt atagatggct
    35461 ggatgaaaac agctatcgtt ttggttttat tcgtcgctac ccaccagaca agacagagat
    35521 aaccggtgtg agcaacgagc cgtggcatta ccgatatgtt ggcatcgaag ctgccacaga
    35581 gatgtacaac caaggggttt gccttgagga atatttaaaa ccagaaaaat gagaaaagga
    35641 tgttatgtta tggacagaaa aagattgaca cagcgtttcc cgttcctgct tccaatgaga
    35701 cgggcgcaga gaaaaatgtg cttttatgcg ggaatgagat ttgacggctg tcgctatgcg
    35761 cagacgatag gggaaaaatc gctttcccat ttgctctttg aaacggattg cgcattatac
    35821 aaccacaata ccggatttga catgatatac caggaaaaca aggtgttcaa cttaaagctg
    35881 gcagcaaaga ccttaaacgg cttattgata agaccggggg aacccttttc tttctggtgg
    35941 ctggtacgcc atgcggacaa agataccccc tataaagacg gccttacggt gaccaatggt
    36001 aaactcacca ccatgtcggg cggcggtatg tgccagatga gcaatttact attttggatg
    36061 ttcctgcata cgccattgac aattatccag cgcagaggtc acgaagtaaa ggagtttcca
    36121 gagccaaaca gtgacgagat taaaggggtg gatgcaacca tttcagaggg ctggattgat
    36181 ttaaaagtgc gaaacgatac cgactgcacc taccaaatat gggtgacctt agatgatgag
    36241 aaaatcatcg gtcaggtgtc cgccgacaaa gagccccaag cattatacaa aattacaaat
    36301 ggcagtatcc agtatgtccg tgaaagtggc gggatttatg aatatgccca ggttaaacgg
    36361 atgcaagttg ccttaggtac cggggaaata atagattgca agctgcttta tacaaacaaa
    36421 tgcaaaatct gttatcccct cccggaaagt gtggatattc aggaggagaa ccaatgagaa
    36481 aaagtgtggg cattactgtt tttggatgcg aacaggatga ggcaaatgct ttccgcgctt
    36541 tatcgccgga ttttcatatt atccctacgc tgattagcga tgcgatatcg gcagacaacg
    36601 caaaattggc cgctggcaat caatgcgtta gcgtaggcca taagtccgag gtttccgagg
    36661 cgacaattct tgcgctgaga aaggtcgggg taaaatacat ttctacccgc agcatcggct
    36721 gcgatcacat tgatacgact gccgccgaga gaatgggaat ctcggttggc acggttgcgt
    36781 attcgccgga cagcgttgcg gattatgctt tgatgctgat gctgatggcc atacggggtg
    36841 caaaacccac catgcacgcc gtggcgcaac aagatttcag attggatcgt atccggggga
    36901 aagaactggg ggatatgact gtgggagtta ttggaaccgg ccatatcggg caagcggtcg
    36961 tcaaaaggct gcggggattt ggatgccatg tgctggccta tgataacagc cgaaaaatgg
    37021 atgcagatta tgtccagctt gatgagcttc taaaaaacag cgatattgtt acgctccatg
    37081 tgccgctttg tgcggatacc cgccatctga tcggtcagaa gcaaattgga gagatgaagc
    37141 aaggcgcatt tttaatcaac accgggcgcg gagcgcttgt cgataccggg gcgctggtgg
    37201 aggcgttgga aagcggaaag ctgggtggtg cggcactgga tgtgttggag ggcgaggatc
    37261 agtttgttta taccgattgc tcgcagaaag tgcttgacca tccctttttg tcgcagcttt
    37321 taaggatgcc gaatgtgatc atcacacccc atacggcgta ctacactgag cgtgtgctgc
    37381 aggatactac agaaaaaaca atcaggaatt gtctcaattt tgaaaggagt ttacagcatg
    37441 aatagaataa aagtcgcaat catcttcggc ggttgctcgg aggaacatga tgtgtcggta
    37501 aaatccgcaa tagaaattgc tgcgaacatt gatacggaaa aattcgatcc gcactacatc
    37561 ggaattacaa aaaacggtgt atggaagcta tgcaagaagc catgtacgga atgggaagcc
    37621 gacagtctcc ccgccatact ctccccggat aggaaaacgc atgggctgct tgtcatgaaa
    37681 gaaagcgaat acgaaacacg gcgtattgat gtggctttcc cggttttgca tggcaaatgc
    37741 ggggaggatg gtgcgataca ggggctgttt gtattgtctg gtatccccta tgtgggctgt
    37801 gatattcaaa gctccgcagc ttgcatggac aaatcactgg cctacattct tacaaaaaat
    37861 gcgggcatcg ccgttcccga atttcaaatg attgataaag gtgacaagcc ggaggcgggt
    37921 gcgcttacct accctgtctt tgtgaagccg gcacggtcag gttcgtcctt tggcgtaacc
    37981 aaagtaaacg gtacggaaga acttaacgct gcgatagaag cggcaggaca atatgatgga
    38041 aaaatcttaa ttgagcaagc gatttcgggc tgtgaggtcg ggtgtgcggt catggggaac
    38101 gaggatgatt tgattgtcgg cgaagtggat caaatccggc tgagccacgg taccttccgc
    38161 atccatcagg aaaacgagcc ggaaaaaggc tcagaaaatg cgatgattac agttcccgca
    38221 gacattccgg tcgaggaacg aaatcgggtg caggaaacgg caaagaaagt atatcgggtg
    38281 cttggatgca gagggcttgc ccgtgttgat ctttttttgc aggaggatgg cggcatcgtt
    38341 ctaaatgagg tcaataccct gcccggtttt acatcgtaca gccgctaccc acgtatgatg
    38401 gccgccgcag gaatcacgct tcctgcactg attgacagcc tgattacatt ggcgttaaag
    38461 aggtgataag tatggaaaat ggttttttgt ttttagatga aatgttgcat ggcgtccgtt
    38521 gggatgccaa gtacgccaca tgggataact tcacaggaaa accggtggat gggtatgagg
    38581 tgaatcgcat catcggcaca aaggccgtgg cgtttgctct gcgcgaggca caaatccatg
    38641 cggctgcgct tggctatggc ttgcttttat gggatggata tcggccaaga actgcggtgg
    38701 actgcttcct gcgttgggca gcgcaaccgg aggacaatct cacaaaagaa aaattttacc
    38761 ccaatataga gcgagccgag ttgattacaa agggttatgt ggcctcacaa tccagccata
    38821 gccgtggaag cgcaattgat cttacgctct accacctgga tacaggggaa cttgtttcaa
    38881 tgggaagtaa cttcgatttt atggacgaac ggtcgcatca tacagcaaaa gggatagggg
    38941 atgcagaggc acaaaatcga agatgcttgc gtaaaatcat ggaaagcagc ggatttcagt
    39001 cttatcgctt tgaatggtgg cactataagt tgattgatga gccatacccc gatacctatt
    39061 ttaattttgc cgtttcataa tgaaagtatt ggttttgtat cagattttgg atttgataaa
    39121 atctatgctg gtcatggaca atcttttact ggaaagtggt taaagcatct tttgtaatac
    39181 attgcaaatt atagtattca atctgcccag cggcagaaaa gaaaaaaacc gctgttgggc
    39241 agacctttcc cacgcccatt ttgtattttg ctggcggcgg ttttgagaaa tcaaggccgc
    39301 cgttcttttc tggcagcaaa aggtgtggcg gcccatagga ggatgccgct atgctgtttt
    39361 gctattgcca tgctctaatc cacttccacc actcaaaaaa agccttgccc ctc
//
LOCUS       DAYYAR010000010        38166 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000010 DAYYAR010000000
VERSION     DAYYAR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.010"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..726)
                     /locus_tag="ACHAT0_000451"
     CDS             complement(61..726)
                     /locus_tag="ACHAT0_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8450424.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(873..1478)
                     /gene="dhaL"
                     /locus_tag="ACHAT0_000452"
     CDS             complement(873..1478)
                     /gene="dhaL"
                     /locus_tag="ACHAT0_000452"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8450425.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(1506..2516)
                     /gene="dhaK"
                     /locus_tag="ACHAT0_000453"
     CDS             complement(1506..2516)
                     /gene="dhaK"
                     /locus_tag="ACHAT0_000453"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8450426.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(2491..2886)
                     /gene="dhaM"
                     /locus_tag="ACHAT0_000454"
     CDS             complement(2491..2886)
                     /gene="dhaM"
                     /locus_tag="ACHAT0_000454"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8450427.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            3040..3360
                     /locus_tag="ACHAT0_000455"
     CDS             3040..3360
                     /locus_tag="ACHAT0_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8450428.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(3472..4329)
                     /locus_tag="ACHAT0_000456"
     CDS             complement(3472..4329)
                     /locus_tag="ACHAT0_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8450429.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(4437..5741)
                     /locus_tag="ACHAT0_000457"
     CDS             complement(4437..5741)
                     /locus_tag="ACHAT0_000457"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8450430.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(5738..6247)
                     /locus_tag="ACHAT0_000458"
     CDS             complement(5738..6247)
                     /locus_tag="ACHAT0_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450431.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(6422..8557)
                     /locus_tag="ACHAT0_000459"
     CDS             complement(6422..8557)
                     /locus_tag="ACHAT0_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8450432.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(8640..10295)
                     /locus_tag="ACHAT0_000460"
     CDS             complement(8640..10295)
                     /locus_tag="ACHAT0_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8450433.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(10297..10611)
                     /locus_tag="ACHAT0_000461"
     CDS             complement(10297..10611)
                     /locus_tag="ACHAT0_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8450434.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            10781..11041
                     /locus_tag="ACHAT0_000462"
     CDS             10781..11041
                     /locus_tag="ACHAT0_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8450435.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            11055..11369
                     /locus_tag="ACHAT0_000463"
     CDS             11055..11369
                     /locus_tag="ACHAT0_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8450436.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(12099..12653)
                     /locus_tag="ACHAT0_000464"
     CDS             complement(12099..12653)
                     /locus_tag="ACHAT0_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8450437.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(12779..14708)
                     /locus_tag="ACHAT0_000465"
                     /pseudo
     CDS             complement(12779..14708)
                     /locus_tag="ACHAT0_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(15014..15730)
                     /locus_tag="ACHAT0_000466"
     CDS             complement(15014..15730)
                     /locus_tag="ACHAT0_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8450438.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(15731..17554)
                     /gene="glpO"
                     /locus_tag="ACHAT0_000467"
     CDS             complement(15731..17554)
                     /gene="glpO"
                     /locus_tag="ACHAT0_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8450439.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(17568..19064)
                     /gene="glpK"
                     /locus_tag="ACHAT0_000468"
     CDS             complement(17568..19064)
                     /gene="glpK"
                     /locus_tag="ACHAT0_000468"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8450440.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(19200..20660)
                     /locus_tag="ACHAT0_000469"
     CDS             complement(19200..20660)
                     /locus_tag="ACHAT0_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8450441.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(20650..21945)
                     /locus_tag="ACHAT0_000470"
     CDS             complement(20650..21945)
                     /locus_tag="ACHAT0_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8450442.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(22277..22954)
                     /locus_tag="ACHAT0_000471"
     CDS             complement(22277..22954)
                     /locus_tag="ACHAT0_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8450443.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22984..24075)
                     /locus_tag="ACHAT0_000472"
     CDS             complement(22984..24075)
                     /locus_tag="ACHAT0_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8450444.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(24176..24610)
                     /locus_tag="ACHAT0_000473"
     CDS             complement(24176..24610)
                     /locus_tag="ACHAT0_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8450445.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(25194..26651)
                     /locus_tag="ACHAT0_000474"
     CDS             complement(25194..26651)
                     /locus_tag="ACHAT0_000474"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8450446.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(26648..28543)
                     /locus_tag="ACHAT0_000475"
     CDS             complement(26648..28543)
                     /locus_tag="ACHAT0_000475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8450447.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(28601..30376)
                     /locus_tag="ACHAT0_000476"
     CDS             complement(28601..30376)
                     /locus_tag="ACHAT0_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8450448.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(30426..31922)
                     /locus_tag="ACHAT0_000477"
     CDS             complement(30426..31922)
                     /locus_tag="ACHAT0_000477"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8450449.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(32116..33738)
                     /locus_tag="ACHAT0_000478"
     CDS             complement(32116..33738)
                     /locus_tag="ACHAT0_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8450450.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(34066..35379)
                     /locus_tag="ACHAT0_000479"
     CDS             complement(34066..35379)
                     /locus_tag="ACHAT0_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8450451.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(35624..35848)
                     /locus_tag="ACHAT0_000480"
     CDS             complement(35624..35848)
                     /locus_tag="ACHAT0_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450452.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35868..36290)
                     /locus_tag="ACHAT0_000481"
     CDS             complement(35868..36290)
                     /locus_tag="ACHAT0_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8450453.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(36432..37142)
                     /locus_tag="ACHAT0_000482"
     CDS             complement(36432..37142)
                     /locus_tag="ACHAT0_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8450454.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(37160..37657)
                     /locus_tag="ACHAT0_000483"
     CDS             complement(37160..37657)
                     /locus_tag="ACHAT0_000483"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8450455.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(37694..38146)
                     /locus_tag="ACHAT0_000484"
     CDS             complement(37694..38146)
                     /locus_tag="ACHAT0_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8450456.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
BASE COUNT        10724 a         8207 c         6445 g        12790 t
ORIGIN      
        1 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
       61 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
      121 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
      181 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
      241 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
      301 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
      361 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
      421 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
      481 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
      541 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
      601 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
      661 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
      721 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
      781 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
      841 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
      901 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
      961 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     1021 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     1081 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     1141 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     1201 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     1261 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     1321 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     1381 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     1441 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     1501 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     1561 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     1621 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     1681 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     1741 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     1801 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     1861 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     1921 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     1981 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     2041 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     2101 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     2161 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
     2221 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     2281 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     2341 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     2401 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     2461 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     2521 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     2581 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     2641 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     2701 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     2761 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     2821 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     2881 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     2941 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
     3001 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     3061 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     3121 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     3181 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     3241 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     3301 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     3361 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     3421 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     3481 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     3541 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     3601 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     3661 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     3721 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     3781 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     3841 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     3901 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     3961 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     4021 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     4081 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     4141 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     4201 agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     4261 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     4321 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     4381 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     4441 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     4501 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     4561 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     4621 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     4681 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     4741 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     4801 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     4861 cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc
     4921 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     4981 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     5041 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     5101 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     5161 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     5221 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     5281 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     5341 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     5401 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     5461 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata ttcgatcaca
     5521 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     5581 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     5641 catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat agacaaacaa
     5701 gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc accccatttt
     5761 ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag acagaataaa
     5821 aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact tcttcaagta
     5881 attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt acttttttgt
     5941 atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa
     6001 tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa aaagcgtaca
     6061 aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat ataaaaggaa
     6121 gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat
     6181 taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct cttgttactt
     6241 ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca tacatttcat
     6301 ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa catttctcat
     6361 cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt ttatctctta
     6421 attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc gccgtgtaca
     6481 tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag attacatgat
     6541 caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct aatccatatg
     6601 aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg gtgataactg
     6661 ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac aattcttcag
     6721 aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat acgatgtcga
     6781 tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg ctagaacggt
     6841 aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa caacgcataa
     6901 tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa
     6961 agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta taatcttgta
     7021 cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca agtaaatgac
     7081 gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa gcataagctg
     7141 agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc catgccattt
     7201 cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt gcataagcta
     7261 caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac tgcctatctt
     7321 ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc aaaagatgat
     7381 tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc aatttaggat
     7441 gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact gtttttccat
     7501 ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct gattgtttta
     7561 gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca ttttctcccg
     7621 tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca
     7681 gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg acattcgaaa
     7741 tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat
     7801 ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt ttaccacttc
     7861 ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt gtgttttgtt
     7921 ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg cctaagactt
     7981 ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc aaacgatctc
     8041 tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt tgacgaaccg
     8101 tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt gaacgccaat
     8161 cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata taaaaggatt
     8221 cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga cttttcaata
     8281 aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc atctgatcga
     8341 tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag taactgtcaa
     8401 aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct atttttgccg
     8461 tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac acgttcttca
     8521 gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc tttttttaaa
     8581 gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt taacgagtat
     8641 catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga aaatgcacca
     8701 tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag atagctacgt
     8761 aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt taatcggtcc
     8821 cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc caattcttct
     8881 gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa ttcatgccat
     8941 tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag catgtttact
     9001 ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg taaatcgaag
     9061 atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc cacacctttt
     9121 gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat caacttcagt
     9181 aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac atgaatgact
     9241 tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga tgcggcagtc
     9301 attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg agaaattccg
     9361 gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag agaaataagt
     9421 gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc ataaatatca
     9481 ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc taattcgatt
     9541 ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat aactaaatca
     9601 gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg
     9661 acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc tataaaagct
     9721 gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac catgacttct
     9781 aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc accaacgatc
     9841 acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc tggtacattt
     9901 ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac aactggtttt
     9961 gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg
    10021 acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg acgcacatct
    10081 aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc ccgttcactg
    10141 atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata cggtcctttt
    10201 tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc agcagacatt
    10261 ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta ctgttcgatc
    10321 aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt tctttaattc
    10381 tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg tcttttcttt
    10441 gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt ttatatgccc
    10501 gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca aaagcttttc
    10561 ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca ttttctgcac
    10621 ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt tattaatatg
    10681 tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa aataccgata
    10741 aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg ataataaaaa
    10801 tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa aaatgatcga
    10861 agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt ctagtattga
    10921 tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga aacctgatag
    10981 taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta ttaaaaagta
    11041 aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa tggtcagaaa
    11101 cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt gtcgtatctg
    11161 taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc ttagatttgc
    11221 aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt
    11281 ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt gaactgcaat
    11341 cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa gtgatcagct
    11401 tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt
    11461 tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
    11521 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    11581 aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa aatagctcct
    11641 catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaagga
    11701 agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggatt
    11761 tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttaa
    11821 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac ggaaaaatag
    11881 ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
    11941 accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa
    12001 cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg gagtctattt
    12061 atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt
    12121 cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa
    12181 atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca
    12241 tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta
    12301 gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac
    12361 aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat
    12421 ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata tctccgagca
    12481 atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa
    12541 aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat
    12601 aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg
    12661 ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg
    12721 atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt tgagataatc
    12781 acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta
    12841 gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat
    12901 aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca
    12961 ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg
    13021 tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc
    13081 gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt gtaactgctg
    13141 tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg
    13201 cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt
    13261 ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    13321 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    13381 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    13441 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    13501 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    13561 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    13621 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    13681 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    13741 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    13801 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    13861 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    13921 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    13981 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    14041 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    14101 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    14161 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    14221 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    14281 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    14341 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    14401 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    14461 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    14521 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    14581 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    14641 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    14701 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    14761 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    14821 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    14881 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    14941 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    15001 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    15061 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    15121 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    15181 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    15241 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    15301 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    15361 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    15421 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    15481 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    15541 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    15601 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    15661 catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt tgagtcatat
    15721 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    15781 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    15841 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    15901 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    15961 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    16021 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    16081 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    16141 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    16201 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    16261 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    16321 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    16381 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    16441 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    16501 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    16561 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    16621 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    16681 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    16741 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    16801 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    16861 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    16921 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    16981 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    17041 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    17101 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    17161 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    17221 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    17281 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    17341 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    17401 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    17461 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    17521 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    17581 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    17641 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    17701 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    17761 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    17821 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    17881 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    17941 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    18001 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    18061 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    18121 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    18181 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    18241 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    18301 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    18361 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    18421 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    18481 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    18541 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    18601 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    18661 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    18721 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    18781 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    18841 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    18901 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    18961 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    19021 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    19081 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    19141 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    19201 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    19261 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    19321 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    19381 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    19441 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    19501 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    19561 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    19621 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    19681 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    19741 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    19801 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    19861 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    19921 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    19981 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    20041 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    20101 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    20161 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    20221 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    20281 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    20341 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    20401 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    20461 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    20521 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    20581 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    20641 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    20701 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    20761 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    20821 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    20881 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    20941 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    21001 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    21061 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    21121 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    21181 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    21241 acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    21301 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    21361 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    21421 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    21481 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    21541 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    21601 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    21661 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    21721 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    21781 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    21841 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    21901 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    21961 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    22021 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    22081 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    22141 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    22201 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    22261 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    22321 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    22381 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    22441 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    22501 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    22561 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    22621 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    22681 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    22741 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    22801 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    22861 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    22921 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    22981 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    23041 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    23101 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    23161 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    23221 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    23281 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    23341 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    23401 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    23461 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    23521 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    23581 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    23641 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    23701 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    23761 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    23821 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    23881 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    23941 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    24001 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    24061 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    24121 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    24181 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    24241 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    24301 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    24361 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    24421 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    24481 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    24541 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    24601 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    24661 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    24721 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    24781 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    24841 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    24901 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    24961 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    25021 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    25081 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    25141 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    25201 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    25261 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    25321 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    25381 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa atagtcaatg
    25441 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    25501 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    25561 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    25621 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    25681 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    25741 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    25801 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    25861 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    25921 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    25981 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    26041 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    26101 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    26161 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    26221 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    26281 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    26341 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    26401 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    26461 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    26521 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    26581 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    26641 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    26701 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    26761 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    26821 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    26881 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    26941 ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg tccgttggaa
    27001 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    27061 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    27121 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    27181 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    27241 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    27301 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    27361 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    27421 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    27481 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    27541 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    27601 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    27661 ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc acgatgatac
    27721 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    27781 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    27841 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    27901 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    27961 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    28021 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    28081 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    28141 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    28201 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    28261 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    28321 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    28381 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    28441 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    28501 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtaccccttc
    28561 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    28621 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    28681 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    28741 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    28801 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    28861 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    28921 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    28981 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    29041 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    29101 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    29161 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    29221 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    29281 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    29341 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    29401 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    29461 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    29521 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    29581 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    29641 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    29701 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    29761 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa
    29821 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    29881 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    29941 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    30001 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    30061 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    30121 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    30181 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    30241 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    30301 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    30361 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    30421 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    30481 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    30541 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    30601 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    30661 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    30721 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    30781 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    30841 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    30901 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    30961 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    31021 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    31081 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    31141 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    31201 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    31261 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    31321 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    31381 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    31441 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    31501 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    31561 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    31621 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    31681 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    31741 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    31801 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    31861 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    31921 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    31981 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    32041 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    32101 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    32161 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    32221 tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    32281 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    32341 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    32401 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    32461 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    32521 cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct cttttgtaaa
    32581 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    32641 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    32701 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    32761 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    32821 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    32881 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    32941 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    33001 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    33061 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    33121 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    33181 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    33241 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    33301 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    33361 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    33421 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    33481 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    33541 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    33601 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    33661 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    33721 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    33781 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    33841 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    33901 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    33961 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact attttttcgt
    34021 ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt tcacattcaa
    34081 tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag atacttcttt
    34141 tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg caatcccatc
    34201 tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga tcgcaatctc
    34261 taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta ccataatcaa
    34321 tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga acaagcctaa
    34381 caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa cggcgatacc
    34441 agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac ccgcaagcat
    34501 agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt ctttcatgaa
    34561 acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag tcacaagcaa
    34621 gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat gttttaatgc
    34681 aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag gcattggaat
    34741 caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga tgccacctgg
    34801 tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca accaagtaga
    34861 accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga tgatcccacc
    34921 ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg catatttcaa
    34981 atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga tcaaaataaa
    35041 aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc ccattcctaa
    35101 acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc caattggata
    35161 acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa
    35221 cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa ataagatata
    35281 agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg tggtgttcaa
    35341 tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc ctaaatccga
    35401 acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt gttttatcat
    35461 atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat ccgaacgttc
    35521 catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat tacaagaatt
    35581 tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag tgtgatttga
    35641 ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca ggattcagat
    35701 tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc ttcttttttc
    35761 ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga tacttcaaat
    35821 aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca atcagacgac
    35881 tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa agtcaagatg
    35941 ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt ttcaaattca
    36001 tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa cttctgcaca
    36061 aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac aatactctta
    36121 aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc tacaaagaat
    36181 cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg tatcatcatt
    36241 tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat tatatcacga
    36301 aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag tttctaggat
    36361 tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta
    36421 tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg tctaacacta
    36481 gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg tgcgccacgt
    36541 gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc
    36601 agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc ttgttcatca
    36661 tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa aggcaggaat
    36721 gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc gatatcttct
    36781 ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac aagcggattg
    36841 attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat agaagaacgt
    36901 gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat gacttcgttt
    36961 tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc attcaataat
    37021 tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt tacggataag
    37081 atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat tacttttttc
    37141 atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct gtttggggat
    37201 catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta cgataaaaag
    37261 cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt tgcttttctt
    37321 ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt ttaaattttt
    37381 ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca gattgactcg
    37441 ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact aggaaaaaat
    37501 cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct tgtgtcaagt
    37561 aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat gtttcgatac
    37621 taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg tatttattca
    37681 cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc caatcttcgc
    37741 taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct tcgtcagaaa
    37801 gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa ggatagattt
    37861 ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta tcttccaaac
    37921 gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt gtacgatcta
    37981 ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa atacgtacta
    38041 aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg ctaatagaat
    38101 ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca tcccaccttt
    38161 attttg
//
LOCUS       DAYYAR010000011        36856 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000011 DAYYAR010000000
VERSION     DAYYAR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.011"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..848
                     /locus_tag="ACHAT0_000485"
     CDS             36..848
                     /locus_tag="ACHAT0_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8450457.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            845..1645
                     /locus_tag="ACHAT0_000486"
     CDS             845..1645
                     /locus_tag="ACHAT0_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8450458.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1806..3074
                     /gene="rseP"
                     /locus_tag="ACHAT0_000487"
     CDS             1806..3074
                     /gene="rseP"
                     /locus_tag="ACHAT0_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8450459.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3142..4851
                     /locus_tag="ACHAT0_000488"
     CDS             3142..4851
                     /locus_tag="ACHAT0_000488"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8450460.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5063..9421
                     /locus_tag="ACHAT0_000489"
     CDS             5063..9421
                     /locus_tag="ACHAT0_000489"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8450461.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9566..10039
                     /gene="rimP"
                     /locus_tag="ACHAT0_000490"
     CDS             9566..10039
                     /gene="rimP"
                     /locus_tag="ACHAT0_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8450462.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10062..11237
                     /gene="nusA"
                     /locus_tag="ACHAT0_000491"
     CDS             10062..11237
                     /gene="nusA"
                     /locus_tag="ACHAT0_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8450463.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11259..11552
                     /locus_tag="ACHAT0_000492"
     CDS             11259..11552
                     /locus_tag="ACHAT0_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8450464.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11549..11860
                     /locus_tag="ACHAT0_000493"
     CDS             11549..11860
                     /locus_tag="ACHAT0_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8450465.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11873..14179
                     /gene="infB"
                     /locus_tag="ACHAT0_000494"
     CDS             11873..14179
                     /gene="infB"
                     /locus_tag="ACHAT0_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8450466.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14206..14553
                     /gene="rbfA"
                     /locus_tag="ACHAT0_000495"
     CDS             14206..14553
                     /gene="rbfA"
                     /locus_tag="ACHAT0_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8450467.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14710..15166)
                     /locus_tag="ACHAT0_000496"
                     /pseudo
     CDS             complement(14710..15166)
                     /locus_tag="ACHAT0_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15331..17693
                     /locus_tag="ACHAT0_000497"
                     /pseudo
     CDS             15331..17693
                     /locus_tag="ACHAT0_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            18061..18984
                     /gene="truB"
                     /locus_tag="ACHAT0_000498"
     CDS             18061..18984
                     /gene="truB"
                     /locus_tag="ACHAT0_000498"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8450468.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18988..19929
                     /gene="ribF"
                     /locus_tag="ACHAT0_000499"
     CDS             18988..19929
                     /gene="ribF"
                     /locus_tag="ACHAT0_000499"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8450469.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20281..21066
                     /locus_tag="ACHAT0_000500"
     CDS             20281..21066
                     /locus_tag="ACHAT0_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8450470.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21079..21930
                     /locus_tag="ACHAT0_000501"
     CDS             21079..21930
                     /locus_tag="ACHAT0_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8450471.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22054..22506)
                     /locus_tag="ACHAT0_000502"
     CDS             complement(22054..22506)
                     /locus_tag="ACHAT0_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8450472.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22971..23339)
                     /locus_tag="ACHAT0_000503"
     CDS             complement(22971..23339)
                     /locus_tag="ACHAT0_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8450473.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23489..23971
                     /locus_tag="ACHAT0_000504"
     CDS             23489..23971
                     /locus_tag="ACHAT0_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8450474.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            24008..25018
                     /locus_tag="ACHAT0_000505"
     CDS             24008..25018
                     /locus_tag="ACHAT0_000505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8450475.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25133..25846)
                     /locus_tag="ACHAT0_000506"
     CDS             complement(25133..25846)
                     /locus_tag="ACHAT0_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450476.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(26019..26626)
                     /locus_tag="ACHAT0_000507"
                     /pseudo
     CDS             complement(26019..26626)
                     /locus_tag="ACHAT0_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            27110..27337
                     /locus_tag="ACHAT0_000508"
     CDS             27110..27337
                     /locus_tag="ACHAT0_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8450477.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27353..28519
                     /gene="hemW"
                     /locus_tag="ACHAT0_000509"
     CDS             27353..28519
                     /gene="hemW"
                     /locus_tag="ACHAT0_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8450478.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28753..29796
                     /gene="hrcA"
                     /locus_tag="ACHAT0_000510"
     CDS             28753..29796
                     /gene="hrcA"
                     /locus_tag="ACHAT0_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8450479.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29890..30453
                     /gene="grpE"
                     /locus_tag="ACHAT0_000511"
     CDS             29890..30453
                     /gene="grpE"
                     /locus_tag="ACHAT0_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8450480.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30504..32333
                     /gene="dnaK"
                     /locus_tag="ACHAT0_000512"
     CDS             30504..32333
                     /gene="dnaK"
                     /locus_tag="ACHAT0_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8450481.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32484..33650
                     /gene="dnaJ"
                     /locus_tag="ACHAT0_000513"
     CDS             32484..33650
                     /gene="dnaJ"
                     /locus_tag="ACHAT0_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8450482.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33732..34361)
                     /locus_tag="ACHAT0_000514"
     CDS             complement(33732..34361)
                     /locus_tag="ACHAT0_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8450483.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            34373..34546
                     /locus_tag="ACHAT0_000515"
     CDS             34373..34546
                     /locus_tag="ACHAT0_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450484.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34591..35328)
                     /locus_tag="ACHAT0_000516"
     CDS             complement(34591..35328)
                     /locus_tag="ACHAT0_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8450485.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(35330..36832)
                     /locus_tag="ACHAT0_000517"
     CDS             complement(35330..36832)
                     /locus_tag="ACHAT0_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8450486.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
BASE COUNT        12371 a         6447 c         7864 g        10174 t
ORIGIN      
        1 atagtgggaa tcttttaagt ataggaggaa aaagaatgtt acgttttttt ccgcaaaaaa
       61 acaaatatat tcaagaaaaa agcgaatttc aatttaatac cgatggacca attcctcagc
      121 acattgccat catcatggac ggcaatggtc gttgggcgca aaaccgtcgt ttgccacgag
      181 ttgctggcca taaagaaggc atggaaacag taaagaaagt cacaaaaaaa gcatcaaggc
      241 ttggagtgaa agtgttgacg ctttatgcct tttcgactga aaactggaag cgaccaaaag
      301 atgaagtaag ttttctcatg caactacctg tcgacttctt tgatacgttc gtaccagaat
      361 tgattaaaga aaatgtcaaa gttcatgtca tgggttatga aaacgtattg ccagaacata
      421 cgcaagatgc tgtaagacgt gcaattgagc aaacaaaaaa caatactgga atggtattga
      481 attttgcttt gaattatgga agcagagcag agatcgtgac tgctgtccaa gaaattgccg
      541 aagaagtagc aaaaggagaa atacacgcag aagaaatcga tgatgaattg attgctaagc
      601 atttaatgac aggtttcttg cctaaagagt tacaagatcc tgagctgatg atccgtacaa
      661 gtggagaaga gcgaattagt aattttctgt tatggcagat cgcatatagt gagctgtatt
      721 ttacaaaagc gctttggcct gattttgatg gtgcgcatct tgaagaagca atcgcctctt
      781 atcaaaatcg agaccgtcga ttcggcggtg taaaaaaaac agaagatgag aaaggggatc
      841 agtcatgaaa caacgtgtgg tcacagcagc cgtggcgcta gtcttgttta ttccgattat
      901 atggatcgga ggcatagcgg ttgagcttac agcagcaatc ttagcagtaa taggagttta
      961 cgagctgttt cggatgaaag gattgacact cttaagtttt gaaggagttt tatccgccgt
     1021 tggtgcagtg ttacttgttt tgccaaaaga aagatggttt ttctttcttc cagagaatgt
     1081 aagtacattt atgttatttt atcttatagt tatgattctt ttaggaacat cagttatttc
     1141 taaaaatacg tatacgattg atgaagcagg ttttcctgtg cttgtcagct tgtatgtggg
     1201 aataggtttc cagaattttg tcaatgcacg aacagaaagc ttggctgttt tgatatttgg
     1261 tctatttatt gtttgggcaa ctgatatcgg agcatatatg gttggtcgac gttatggtca
     1321 gcataagtta agtcctgata tttcgcccaa caaaacgatt gaaggtgcgc tcggtggtat
     1381 tggtagtgct gttgtagtcg catttcttta tttattgcta tacccagcaa aagaactatt
     1441 taatcatggt atgtttgtca tgcttctgat gacgattatt ttttcagcag tcggacaatt
     1501 tggtgatttg gtcgaatctg caatcaaacg tcattatgat gtgaaagact cgggaaatat
     1561 cttaccaggc catggtggaa tcttagaccg tttcgacagt ttgctgtttg tatttcctat
     1621 catgcattta tttggcatta tttagtaagt atacctgcgt ttctttgtga atttcataaa
     1681 agacgcaggt ttttattttt cattatattg atactattgg tgtctttggt atactatggt
     1741 aaaattatag tgttttatcg ttgttttata aaaaacacaa aaaacaagta aaggagaaaa
     1801 aagctatgaa aacgattctg acatttatca tcgtttttgg tatattagtg attgttcatg
     1861 agtttggtca tttcttcttt gcgaaacgat caggaatcct cgttcgcgaa tttgctatcg
     1921 gtatgggtcc gaaaatttat gggcatcaag caaaagacgg gacaacatat acgcttcgcc
     1981 tattacctat tggcggttat gttcgaatgg caggcaatgg ggatgatgaa acagaaatgg
     2041 caccaggaat gcctctttct cttttactaa attctgacgg catcgtagag aagataaatt
     2101 taagtaagaa aatccaattg acaaatgcta tcccgatgga attgagccgt tatgatttag
     2161 aagatgagct gactatcact ggttatgtga atggagacga aacagaagta gtaacctacc
     2221 ctgttgatca cgatgcgacg attattgaaa acgacggaac agaaatcaga attgcgccaa
     2281 aagacgtaca gttccaatcg gctaaattgt ggcagcgtat gctgaccaac tttgctggac
     2341 ccatgaataa ctttattttg gcaatcctgt tattcatcat tctggcattt atgcaaggtg
     2401 gtgttcaagt gacgaacact aatcgcgtag gagagatcat gcctaatgga gctgcggcag
     2461 aagcaggctt aaaagaaaat gatgaggtag tcagtgtgga cggaaaagaa atccattcat
     2521 ggaatgatct gacaacagtc attacgaaaa atccggataa aacattagac tttaagatcg
     2581 aacgagaagg tcaggtgcaa tctgtcgacg tgacaccgaa atctgtagag tcaaatggcg
     2641 aaaaagttgg ccaacttggt ataaaagccc caatgaatac aggttttatg gataagatca
     2701 ttggagggac gcgtcaagct ttcagcggtt cattggaaat tttcaaagcc ttaggatcac
     2761 tatttacagg tttcagttta gataaattag gcggacctgt catgatgtac caattgtctt
     2821 cagaagcagc aaatcaaggt gtaacaactg ttattagttt gatggcgctt ctttcaatga
     2881 atctcggaat cgtcaatctg cttccgattc ctgccttaga tggcgggaaa ttagtcttga
     2941 atatttttga agggattcgc gggaaaccat taagtcaaga aaaagaagga atattgacat
     3001 tagctggctt tggattttta atgttgttga tggtgctagt tacatggaac gatattcaac
     3061 gattcttttt ctagaaaaag ttgacaaata gggtataata acaaacgata actattttga
     3121 ggaaataaag gagtatatag aatgagacaa tcaaaaatgt tgattccaac attacgcgaa
     3181 gttccaaatg atgcagaagt attaagccat caaatcttgc taagagctgg atatatccgc
     3241 caagtatcag cgggtattta ttcatatctg ccattagcaa atcgtgtatt ggaaaaactg
     3301 aaacgcatta tgcgggaaga atttgaaaaa atcggagcgg tagaaatgct gatgcctgcc
     3361 atcttgccag cagaactttg ggaagaatcc gggcgttacg aaacatatgg accaaacttg
     3421 tatcgtttca aagaccgcaa tgatcgaaaa atgatccttg gaccaactca cgaagaaact
     3481 tttactgatt tgatgcgtaa tgaaatcaat tcatataaga aattaccttt aaatctttat
     3541 caaatccaac ctaaatatcg tgacgaaaaa cgacctcgct tcggtttgct tcgcggaaga
     3601 gagtttatca tgaaagatgc gtattctttc catgcaacgg aagaaagttt ggacgagaca
     3661 tataaagatt acgaaaaagc ctacaccgaa gtattcaagc gttgtggctt agaatttcgc
     3721 tcgattatcg gagacggtgg cgcaatgggc gggaaagact caaaagaatt catggcgatc
     3781 tcagaaatcg gggaggatac gatctgttat tcgactgaaa gtgattatgc tgcaaacttg
     3841 gagatggcta caagctacta tgtatcaaaa aaatcgcatg aaacacagct tgaattggag
     3901 aaagtagcta cacctaacgt aaaatcaatt gacgaagtag cagatttctt ttctgttgaa
     3961 cctcaaaaaa tcatcaagtc tgtattgttc attgcggatg aacaacctgt ccttgttttg
     4021 gtacgcggag accatgaagt caatgacgta aaactgaaaa actttttatc tgctgacttt
     4081 ttagaagaag caacagaaga agaagcacaa aaatatctag gtgctgagtt tggttctgtc
     4141 ggaccagtcg gtgtttcaga tgacgtaaaa gtatatgcag accgttatgt ccaagattta
     4201 gcgaatgcca ttactggagc gaacgaaaca ggattccact ataaaaatgt gaatccagaa
     4261 cgcgatttca ctgtactttc ttatgaagat cttcgttttg tacaagaagg agatccttca
     4321 ccagacaaca atggtgttct tgcctttact cgaggaatcg aaatcggaca tatcttcaaa
     4381 atcggcactc gttacagtga atctatggga gcaactgtac tagacgaaaa tggacgagaa
     4441 aaatttgtga ttatgggctg ttatggtatc ggtgtaagtc gtttattatc agcaatcgta
     4501 gaacaacatt cagatgaaca aggaatcaac tggccaaaag gaattgcgcc atttgatttg
     4561 catatcgtcc aaatgaatct aaaagacgaa taccaaacaa acctgacgaa tgaactagaa
     4621 gaagcgatga caaaagctgg ctatgaagta ctggttgatg acagaaatga acgcgcagga
     4681 gtgaaattcg cggattctga tttgattggc tgtccaatca gaatcactgt cggtaaaaaa
     4741 gcagtggatg gaatcgtaga agtgaaaatc aagaaaactg gcgaaatggt tgaagtacgt
     4801 aaagaagaat tggtgaatac tttaccaatc ctcttgaacc aaggcgaata ataagcgtta
     4861 aaacacagaa aataaaaata gctgaatcag ctgtttttga caatctgatg attcgctttt
     4921 aaaattggaa ctgcgatttg aatcgcagtt ccaatttttt aatgtaaaac tcagaaaaaa
     4981 cgagtatact aaaaggtact gaaaaagaag aggataagat gcgatcaatt gcattgtttc
     5041 tcatcagaaa gggcgtatat ctttgtcaga aaaacgagat ttatttgaaa aactgttgga
     5101 acaaatccaa ctagaggaag cagaaaaaaa tcatccactg cttactgctg gtgaaatgga
     5161 tagtgtagtg gtccatcgta aatcgcgttt atgggaattt acgctgcatt tttccaaaat
     5221 tttgcctatt atgctatatc ggtcattgac tcagcatctg actatcgctt ttaaagatat
     5281 tgcacaagtc aaactgaata tcttagcaga tgaccagaca ttcgatgaac agctcttgca
     5341 agattactgg gcccaagctt tggaaaatca gcaatgcgat acaccactag cacagcaagt
     5401 actgaaaacg caggtacctg tgataaaaga tcgtaaagtc atcttgccaa tcgacagtac
     5461 aggagctatt tcttatttga agcaacaata cttgccatta gtagaagagt tgttcgtcag
     5521 ttacggattc ccaaaatttc gtatcgaacc agaagtggat gagcagcaag cggaacgagt
     5581 attgaaactt tttgaagaac gcaaacaaga acaggcagaa gcctttatga agcaagcagc
     5641 tgaaagtcta gttgtccacg aacaaaagaa aaaggaaaga aaagagcaat cgccagctct
     5701 tgaaggaccc atccacatcc agctaggacg aaatattcca gccgatgaac caacgacacc
     5761 aatgatcagt atcgttgaag aagaacgaag agtcacactt gaggggtacg tattcgacaa
     5821 agaagtcaga gaactacgtt caaaacgcaa gatcttgact ttgaaaatca cagattatac
     5881 ttcatcattt atcgtcaaaa aattctcgaa cggcgaaaaa gatgaacaag tatttgatgc
     5941 gatcagtgtc ggtagctggt taaaggtacg aggaagtatt caagaagata cgtttgtccg
     6001 tgatttggtc atgaatgcgc aagatatcat agaagtcaaa catacacctc gcaaagatta
     6061 tgctcctgaa ggggaaaaac gagtagagct tcatgttcat agcaatatga gtacgatgga
     6121 tgcaacaaac agcatctctg atcttgtggc acaagcaggg aaatggggac accgagctat
     6181 cgccattaca gaccacggtg gtgcgcaagc ctttccggaa gcacatagtg ctggaaaaaa
     6241 agcaggtgtg aaaatcttat acggcgtgga agcgaatgtc gtagatgatg gtgtcccaat
     6301 tgcttataat gatgcacatg aagcattgag tgaagccaca tatgtcgtat ttgacgtgga
     6361 aacgacagga ttgtctgcag tatatgacac gatcatcgag ctagccgcag tcaaaatgta
     6421 taaaggaaat gtgatcgaaa gctttgatga atttatcgat ccaggacatc ctttgtcacg
     6481 aacaacggta gatctgactg gtatcacgga tggaatggtt cgaggatcaa agtcggaaga
     6541 agaagtattg cggatgttcc ttgaattttc aaaagataca attttggtag cccacaacgc
     6601 ggcttttgat atggggttct tgaatactag ctacgcaagg tatggtattc ctgaagcggc
     6661 caatccagtc attgatacgt tggaattagc tagatatctg tatcctcaat tcaaacgttt
     6721 cggattaggt gtgttatcta aaaaatttgg tgtcagcttg gaacagcatc accgagcaat
     6781 ctatgatgct gaagcaactg gtcacttagc atggatcttt gtgaaagagg caatggacaa
     6841 tcataacatg ctttatcatg atcagctaaa tgagcatatc ggagaaggag attcttacaa
     6901 aagggctcga cctttccatg taacgatttt agcaaaaaat caggcaggac tgaaagattt
     6961 gttcaaactg atttcaatgt cgaatgtcga atacttcgaa cgcgtcccac gaataccacg
     7021 ctcccaattg aaaaagatgc gtgaaaatct attgatcggc tcagcttgtg ataagggaga
     7081 aatattcgaa gccatgatgc aaaaaggggt agaagaggca agaaaccgag caaaattcta
     7141 cgattatatt gaagttatgc caaaagcggt ctatgcgcct ttgatcgagc aagaattggt
     7201 aaaaaatgaa catgatttgg aagaaattat ccaaaatttg gtagaaatcg gaaaaagttt
     7261 ggataaaatc gttgttgcaa caggtaatgt acattatctg aacgaagaag atgccattta
     7321 ccgaaaaatt ttgatcaatt ctatgggcgg agcgaatccg ttgaatcgtc atagtttgcc
     7381 ggatgtccat ttccgaacaa ccgatgagat gctaactgca tttcactttt taggggaaga
     7441 aacagcgaaa gagatcgtag tagaaaatac gaataaaatc gcagatatct gcgaagaagt
     7501 gattccagtc aaagatgaac tttatacacc gaagattcca ggttcagaag atgaaatatc
     7561 agaattgagt tacacaaaag caaaacaaat gtacggagat ccgttacctg agatcattca
     7621 aaaacgatta aaaaaagagt tgaattctat taatggtaat ggcttctcgg ttatttatct
     7681 gatcgcccaa aaactagtgc ataaaagtaa tgaagatggc tatctagtag gttcacgtgg
     7741 ctctgttggc tctagctttg ttgcgacgat gacaggaatc actgaagtta atccgttagc
     7801 ccctcattat tattgtccag agtgccaata ttccgagttt tttgaagatg gtacgtatgg
     7861 ttctggattt gacatgccag aaaaacaatg tccaaaatgt ggtgcacgct taaacaaaga
     7921 cggtcatgat attccatttg agacattttt aggattccat ggagacaaag tgcccgatat
     7981 tgatttgaac ttctcaggtg attatcaagc agaagcccat aactatacga aagtattgtt
     8041 tggcgaagac tatgtttacc gtgccggaac gattggtaca gtcgccgaca aaacagcata
     8101 tggctatgta aaaggatacg aaagggacaa taatctgcag tttcgaagtg cagaagtcga
     8161 cagattagca aaaggcgcta ctggagtcaa acggacaacc ggccagcatc caggggggat
     8221 catcgttatt cccgattata tggatgtcta tgattttacg ccgatccaat atccagcaga
     8281 cgatcaaaat tcggaatgga aaacgactca ttttgatttt cactcgattc acgataatgt
     8341 attgaaatta gatattctag ggcatgatga cccaactgtt attcggatgc ttcaagattt
     8401 atcagggatc gatccacaaa cgatccctac ggatgacccg gaagtgatgc ggatatttgc
     8461 cggtcctgaa gtacttggag taagtcagga acaaatttat tccaaaacag ggacattagg
     8521 tattccagaa ttcggcactc gttttgttcg agggatgcta gaagaaacgc atcctacaac
     8581 ttttgcagaa ctactgcaaa tttccggact atctcatggt acagacgtat ggttaggaaa
     8641 tgcggaagaa ttgattcgac gaggagatgc gacactggcg gaagtaatcg gctgtcgtga
     8701 tgatattatg gtttatttga tccatgctgg attagatagc gggatggcat ttaaaatcat
     8761 ggaaactgtt cgtaaaggac aatggaacaa gatccctgat gaattgcgag aaacctatct
     8821 atcggcgatg aaagaaaata atgtgcccga ctggtatatc gattcttgtt caaaaatcaa
     8881 atatatgttt ccgaaagccc atgctgcagc ttacgtattg atggctcttc gtgtcgctta
     8941 tttcaaggtt tatttcccta ttttgtatta ctgtgcatac ttttctgtac gtgcagacga
     9001 ctttgatcta gtctccatgt gcaaaggaaa agatgctgtg aaacaagcaa tgaaagaaat
     9061 cacggacaaa ggattagatg cttccgtaaa agaaaagaat caactgacag tgctggagtt
     9121 agccaatgaa atgctggaac gaggattcaa atttggaatg attgatttgt ataaatcgga
     9181 tgccgttaac tttgttattg aaggtgatac gttgatcgca ccatttagag ctgtcccaag
     9241 tttgggaacc aacgtggcaa aacaaatcgt tgaagcaaga aaagacggtc catttttatc
     9301 caaagaagat cttgccaccc gcggtaaagt ttcaaaaaca ttgatcgaat atatgaacga
     9361 caatggcgta ttgaaagatc taccagatga aaatcaatta tcgttattcg atatgctata
     9421 agcatctgtt ttcctgtctg cttgtaattt taagcagagt atggtatagt tcttataggt
     9481 aatgatacta actgtgagtg agcggatttt tcgctcactc tttttaatgg aattacgcac
     9541 ttaatttttt aaggaggcga aacttttgag tagcgtcgtt gaaacagtca cagaaatggt
     9601 gacaccgatt ttagatgaac aaaattttga acttgtcgaa gtcgaatttg taaaagaagg
     9661 aaaaagctgg ttcttacgag tatttattga caaagaaggc ggtatcgata tcgaagaatg
     9721 cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca gcagatccag atccgattcc
     9781 tcaagcttat ttcctggagg tatcctcccc gggagcagag cggccattga aaaaagaaag
     9841 cgactatgaa aaggcacttg gtgattatgt ccatgtctcc ttataccaac cagtagatgg
     9901 cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct gaacagctta cattgaaaat
     9961 acgcataaaa acacgagaaa aagaattgac ttttgaccga aaaaatattg caaaagctcg
    10021 cttagctatc caattttaat cacggagagg aatcagaaaa aatgagtaaa gaaatgttaa
    10081 atgcgttaga cgctttagaa gctgaaaaag ggatctcaaa agaaatcgtg atcgatgctt
    10141 tagaagcagc attggtttct gcatacaaac gtcactatgg ccaagcacaa aacgtcgaag
    10201 tggaatttga tcagaaaaaa ggaaagatcc atgtctatgc ggtaaaagaa gtaacggaag
    10261 aagtgatgga ttcacagttg gaagtctcat tgaaagatgc acttttaatc aatccagcat
    10321 atgagatcgg tgatacgatc cgttttgaag taacaccaaa agattttggg cggatcgctg
    10381 cgcaaacggc taaacaagtt attttacagc gtgtacgtga agcagaaaga acgattattt
    10441 ataacgaatt cagtgcttat gaaaaagaca tcatgcaagg aatcgtcgaa cgccaggata
    10501 aacgttatat ttatgtcaat cttggtaaaa ttgaggcagt tctttctaaa caagatcaaa
    10561 tgccaaatga attttatcaa ccacatgatc gaatcaaagt ttatgtttcc cgagtagaaa
    10621 atacgtctaa aggtccgcaa gtatttgtta gccgcagcca tccagaccta ttgcgccgct
    10681 tatttgagca agaagtacca gaagtctatg atggaatcgt tgaaattgtc agcatcgccc
    10741 gtgaagctgg cgatcgttca aaagtggcag ttcgctcaaa tgatccaaat atcgatgcag
    10801 tcggtacatg tgtcgggcca aagggacaac gagttcaagc aatcgtcaat gaattaaaag
    10861 gcgaaaacat ggatatcgtc gaatggaatg aagatccagc tgtattcatt gcaaatgcat
    10921 tgaatcctgc acaagtcatg gatgtgatct tcgatgagac aaatccaaag gcctgcacag
    10981 ttgtcgttcc agactaccaa ttatcgctag caatcggtaa acgcggacaa aacgcacgtt
    11041 tagctgctaa attgactaac cataaaatcg atatcaaatc agaatctgac atgacggaat
    11101 tttatgaaaa tcaagaacaa caaaaagaaa cagaagaact gcatgatgaa gcaatcgttc
    11161 agtcagattt aacagacgat gaatatgaaa caattgcatt caacaatgaa acggtcgaag
    11221 aaaaaccaga agcttaactt tagtaaggag gcaaagagat gaaacaaagg aaaattcctt
    11281 tacgcaaatc cgtggtttca ggtgaaatga aacctaaaaa agagatggtc cgaatcaccc
    11341 gttcaaaaga aggggaagta tcgatcgatc caactggaaa aatgcctgga cgaggagcgt
    11401 atgtctcact tgagccggaa gaagtacaac aagcttggga taaaaaaatt ctcgatcgag
    11461 tactcgaaac aacattaaca gatgagtttt atcaagaact tcttgactat gtcactcacc
    11521 aaaaagcacg taaagagtta tttggaaaat gaatacagtt aatcggcaaa aagccatgaa
    11581 tctcatcggt ctagcgatgc gcgcgggaaa actaatcacg ggtgaagaac tgaccattgc
    11641 tgaaatccgc aaacaaaaag caaaattggt gtttgtggca accgatgcaa gcgagaatac
    11701 aagaaaaaag ataaaagata agagttcata ttatgaagtt ccttgctttg agctattttc
    11761 tgaagcagaa atcacgcaga tgatcggaaa acctcgcaag gtattcggaa taatggatgc
    11821 cggatttgcg aagaagacca aggagctaat tgaaggttag gaaggtgatt gcatgggcaa
    11881 caaaagaatc tatgaattag caaaagaact caaaaaatct agtaaagacg tcgtggaaaa
    11941 agcacaacaa cttggcatcg atgtgaaaaa ccatatgggt gcgattacta gcaatgatga
    12001 gaagaaattg caacaagcgt tcaaaaaccc aagcacgaaa caacaagctg cacaacaagc
    12061 aagcagaaaa ccagccaatc aacagccgaa cgatcaacgt aaaaaagaaa caacgaacaa
    12121 tcaacaaaaa aataaaaata accgcagtta tcaagatcgc ggtcaaggga gcggtcaagt
    12181 gaatcaagga aaaaaaccaa caacaaacca aaataatacg ggaaatacca gcagccaaaa
    12241 tcgtcaagga acgagccaag gcggcaatca aaaccgtact aacaataaca acaaaccaaa
    12301 caataacaat cgcggcaaat ttaataataa taaccgtaat cgttataata aaaaaggtaa
    12361 aaaaggcaaa cagcaacaat caactaaacc agctgtacca ccacgcaaat tccgtgaatt
    12421 gccagatgta ttagagtata ctgaaggtat gaacgtagca gatattgcga aaaaaattca
    12481 tcgtgagcca gcagaaatca ttaaaaaatt atttatgctt ggtgtcatgg tcaatcaaaa
    12541 ccaagcactg gataaagata cgattgaact gttagcgacg gactatggca tggaaccaca
    12601 agaaaaagtc caagttgaca ttgctgatat cgataaattc ttcgaggcag aagaagtcga
    12661 tccagataaa ttagtctcac gtccgccagt cgtaacgatt atgggacacg tagaccatgg
    12721 gaaaacaact ttattggata cattacgcca ttcacgtgta acaagcggcg aagccggcgg
    12781 aatcacacag catatcggtg cctaccagat cgatattgat ggtaaaccaa tcactttctt
    12841 ggatacacca ggacatgcgg cctttacaag tatgcgtgca cgtggagcaa gtatcacaga
    12901 tatcacgatc ttagttgttg ctgcagatga tggcgtcatg ccacaaacag ttgaagcaat
    12961 caaccatgcc aaagctgcag gcgttccaat catcgtcgct gtaaataaaa ttgataaacc
    13021 aggtgcaaac cctcaacatg ttatgcaaga attaagtgag tatgagttaa ttcctgaagc
    13081 atggggtgga gaaacgatct ttgttgagat ctcagcgaaa ttcggccaaa acatcgaaga
    13141 attgctggaa atgatcctgt tagtcgcaga agtagaagat ttaaaagcag atccaacaca
    13201 acgcgcaatc ggaacagtga tcgaagcacg tcttgataaa ggtaaaggtc ctgtgtcaac
    13261 cttacttgtc caagaaggaa cacttcatgt tggtgaccca atcgttgtcg gtaatactta
    13321 cggacgtgtc cgtgtaatga caaacgatct tggccgtcgc gataaagaag caggacctgc
    13381 aacgccagta gaaatcactg gcttgaatga tgtacctcaa gcaggtgacc gtttcgtagt
    13441 cttcgaagac gaaaagacag cacgtgcagc gggtgaagaa cgcgcgaaac gtgcattatt
    13501 agaaaaccgt gctgccacaa gccgtgtgac tttggataac ttatttgaaa gcctgaaaga
    13561 aggcgaattg aaagaagtta acgtaatcat caaagcagat gtacaaggtt cagcagaagc
    13621 actggctgca tcattgaaga agattgatgt ggaaggtgtc cgtgtaaaaa tcgtccattc
    13681 agctgtcgga gcaatcaatg aaagtgatgt aaccttagca gcagccagca acgctattat
    13741 catcggtttc aacgttcgtc ctacaccgca ggcaaaagtg caagctgata ctgaagaagt
    13801 cgatattcgt cttcaccgta tcatctataa agccatcgaa gaaatcgaaa ctgcgatgaa
    13861 agggatgcta gaccctgaat ttgaagaaaa aatcaccggt caaatgatcg ttcgtgaaac
    13921 cttcaaagtt tctaaagttg gtacgattgc aggtgcctat gtaacagaag gatatattcg
    13981 tagagatagc ggtgtccgtg taatccgtga cggaatcgta atctatgaag gccaacttgc
    14041 aagtctgaaa cgattcaaag atgacgtgaa agaagtcaaa atgggttatg aatgtggtgc
    14101 aatgattgaa aaattcaatg acatcaaagt agatgacgta attgaaggat ttatcatgga
    14161 agaagtcaaa aatgactaaa aactgataaa aggaggccaa tcattatggc taactaccgt
    14221 gaccgccgag tcggtcaaga aatcttaaaa gaagtcaatg atattttgcg caaaaaggtc
    14281 cgtgaccctc gtgtggagaa cgtaacgatc acagatgtga aagttacggg ggacttacag
    14341 caagcaacga tctattacag cattttatct gacttagcat cagataagaa aacagcacaa
    14401 gatggattga ataaagcatc aggattgatt cgtcgagagt tagggcacaa tctaagtatt
    14461 tataagacac ctgaattgtt ctttgaactt gatgaatcag ttgtctatgg caaccatatc
    14521 gatgaattgc ttcgcgactt gaacaaagac taatcaaaaa ctcccgcaaa tcctgtgaaa
    14581 tcaggggttg cgggattttt ttgttttcat taagacgtat tacctaagag ttccatgact
    14641 ttagtcttgc ggcgtgtcag ctaaccaaaa aagagaaaaa gaaaagtaaa aaattcgact
    14701 ttcttttttc tagtagtata taaatttttg catatagata aagatcaaga taccgataac
    14761 ggaagctaga ttgtaatcta ttttagtgac agtcaggtat agccaagtaa ctagaagatc
    14821 agtggaacct gcaccataaa agtttgagtt tgtcggtccg ttgacagtta aaagaaaaat
    14881 cacgttgaag ttattgatat ttccgattac gccgcttcga tttggtcacg atccaaattt
    14941 tggataatcc ctgtagaaat tagcatagta gctgggatac caattcacat attgacaaag
    15001 ataacagaga cttttacata aaacggatca atcaggaaag gaattgctcg atccagcccc
    15061 agttttccag caatgtgtta actggcccag cgtcattcag caaattatgc atgatgagga
    15121 gcgaaatgaa ctgcggaaca gccattgtga taacgacaag ttttttggat caatgtaaaa
    15181 aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct gttcatttgg ctagatataa
    15241 aatccaaaca aatgaacaga agtgcttatt aggtttcaat tgggagtatc tgcgctagaa
    15301 taagagtaat aatgaaaagg agtgttatgt atggattatt ctagtaaaga atactttgat
    15361 aaaatgacgg cttggtggcg tgctgctaat tatctttctg ttgggcaact ttatcttaaa
    15421 gataacccct tactgagacg tacactgaaa cccgaagatg tcaaaaaaca tccaatcggt
    15481 cattggggaa cgattcctgg ccaaaatttt atttatgtcc atttgaaccg agtcatcaat
    15541 aaatatgatt tgaacatgtt ttatattgaa gggccaggac acggcggaca agtcatggta
    15601 tcgaatgctt atttagatgg aagttacaca gagatctatc cagaagtaac ggaagatgag
    15661 acaggaatgc aaaaattatt caaacgattt tcatttcctg gaggcattgc ttctcatgca
    15721 gcaccagaaa caccaggttc tatccatgaa ggcggagagc ttggatactc tctttcacat
    15781 gcagtcggag ctgtattaga caatcctgaa gtaatctctg ctgtcgtgat tggtgatggt
    15841 gaagcagaaa ccggtccttt agctggaagc tggttctcaa atgtctttat caatccagtg
    15901 atagatggag ctgttttacc gatccttcat ttaaatggtg caaaaatagc caacccaacg
    15961 atcttggcac gtaaatcaga tggagaatta gcaaattact ttaacggact tggctgggaa
    16021 cctttcttta ttgaaggaaa tgattcagaa aaattaaatc ctgttatggc tgaaaaaatg
    16081 gatcaagcaa tcgagaaaat taaatccatc caaaaagaag ctcgcctgaa gacagcaaca
    16141 gatgtcgtga tgccaaaatg gccagtattg atcgtacgca caccaaaagg atggaccggt
    16201 ccaaaagaat gggacgggga accaattgaa ggaaccttcc gagcacacca agtaccgatt
    16261 cctgtagatc aagaacatat ggatcatgca gatgctttac tccgttggct gaaatcctat
    16321 gaaccagaaa aattatttga tgcacaagga cgtattttag aagaaatcag agaaatcgcg
    16381 cctactggtg accaaagaat ggcaaaaaat cctatcacca atggtggaat cgatccaaga
    16441 ccgcttatta tgccagattg gaaaaaatat actttgcaat ttgaaaaacc aggctccgtt
    16501 aaagcagaag atatgaccga acttggtaaa ttcgttcgcg agatcattga gaaaaatcca
    16561 gaaaatttcc gtatatttgg accagatgaa acaaaatcaa accgattgaa tcaagttttc
    16621 aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag aaaatgatga atggctttcg
    16681 ccatctggtc gcgtcatcga cagtcaatta tcggaacacc aagatgaagg gttcttagaa
    16741 gggtacgttc tgactggacg gcatggtttt ttgcaagtta cgaatcattc ttgcgagtgg
    16801 ttgactctat gttgactcag cacttcaaat ggatgcggaa atcacatgac ctttcttggc
    16861 gaaatgatta tccttcgcta aatctcattg caagctcaac agtcttccag caagatcaca
    16921 atggatatag ccaccaagat ccaggaatat tgacacattt ggcagaaaag aaggcggagt
    16981 tcatccgtga atatctgcct gcggatgcaa atacattgct cgcagtcatg gataaagcat
    17041 tccgctcgtc agaaaaaatc aatttgatca tctcttccaa acatccgcgt gctcaatttt
    17101 actcagcaga agaagcagcc gtcttagtga atgaaggctt gaaaatcatt gattgggctt
    17161 cgacagcaaa agaggaggaa cctgaattgg tcattgctgc tgcaggaacg gaatctaact
    17221 tagaagcatt agcagctgta actttgctgc tggaagaatt cccaaaacta aaaattcggt
    17281 tcatcaatgt cgtagattta ttgaaattac gtcatccaag tcaagatccg cgtgggttga
    17341 gcgatgaaga atttgatcaa tattttacaa aagacaaacc gatcttattt gctttccacg
    17401 gttatgaaac attggtccgc acgattttct ttgatcgtca taaccatcat ttaatgattc
    17461 atggctacaa agaaaatgga gatatcacga caccatttga catgcgtgta gtaaatgaac
    17521 ttgatcgtta tcatttggca aaagacgcag ctttaaaaat caaaggaagt caagcagaag
    17581 attttgcgaa aaaaatggat cagaaattac aagaacatca aaactatatt cgtgaaaatg
    17641 gtattgatct gccagaagta ttggattgga aatggaaaaa tcttgaccaa taaaatgata
    17701 aaatcgatcc cgcaaaatag aattttttac attgattaag aaactgaaaa aataaataaa
    17761 aaaaacggaa aaaagactgg aagaatggcc agtctttttt ttcgtttttg ggaagtgaaa
    17821 accaaactat ttacctgtaa gccacccata aatccaatca aatggtgcaa agaaaaaatc
    17881 ttccattcta ttccctcctt taaaaaagca taacatgatt tctatttacg tgagcatgaa
    17941 ttttgttacg tttttattgc cgaaaaataa aaaccgttca ttatgatgtc aaattcgtca
    18001 gtaattgatg caatgtgtta taataattca gttaaatcaa aaatgaaatg gagaaaagac
    18061 atggacgggt tattaccttt atggaaagaa cgcggaatga caagtcatga ttgcgtattc
    18121 aaattaagaa aaatcttaca tacgaaaaaa atcggacatg ggggtacgct tgacccagac
    18181 gtagacggtg tgttgcccat ttgtattgga aaagccacaa aagttatcga atatttgaca
    18241 gattctggga aaatctatgt aggcgagata actctaggtt tcagtacaac gacggaagat
    18301 cgatccggag aacgggtaga aacaaaacca gtagatatgt ctttatctga aaaaaaaata
    18361 gatgaagtga tggcttcatt tattggtgag atcacccaga taccaccaat gtactcagcg
    18421 gtaaaagtca acggtaagcg gttatatgaa tatgcccgca ataatgaaac agtggagcgt
    18481 cctgttagaa aggctcagat ttaccgtttt atccggacaa gtgaattgac aagagacgaa
    18541 gtgacaggaa ccatttcttg gcgcttcgaa gtagaatgcg ggaaaggaac ctatgtccgc
    18601 acattagctg tagatacagg caagaaactt ggttaccctg cgcacatgtc tgatctgacc
    18661 cgaacagcaa gtgccggtat gtcccagaag gatgctctga cattagaaga agtagctcaa
    18721 gctgtgaaaa atgggacaat cgatgattgt ttgctgccaa tagaaaaagg agtggaacgg
    18781 ttcaaacgag tagatattac tgatgaagta tggcagaaag taaaaaacgg tatgagactt
    18841 gattatactg tatttgcgct cccagaaatg ccgtcagaag aaattgcttt gttttataaa
    18901 ggaaaagttg tcagtattta tcagccaaac ccgaaagaaa aaaacaaatt aaaacctagt
    18961 aaagtattga gaaatgagga ataacgaatg aatatcatca agatcagaca tccttaccaa
    19021 tcagaacaaa ttccagaaga cgatgtcgtt ttagtgctgg gatttttcga cggcgtgcat
    19081 atcggacacc agaaagtcat aaagacaggt atggaaattg cacaaaaaga aggattgaaa
    19141 ttagcattga tgactttcaa tcagcatcca tctattgtct ttaaaaagat cgatcctagc
    19201 agcgtgaaat atttgactag ccttaagcaa aaagaagaaa aaatggccgc tttgggtata
    19261 gattatttat atgaagttga ttttacttct tcttttgctc atttagcacc gcaggaattt
    19321 gttgatcaat acatcgtagg gttacatgca aaatatgctg tttccggttt tgattatacg
    19381 tatggaccaa aagacattgc agatgtcgct cattttcctg aatatgccaa aggaagattc
    19441 aaaatcgtca cagtttctaa agaagaagaa caaggtgaaa aaatcagctc tacccgtatt
    19501 cgtggattac tagacagcgg acaagtaaaa gaagcagcaa aactattagg ttgcacatat
    19561 gaggtagaag gggtagttgt ccatggcgat gcacgaggca gactactagg attccctaca
    19621 gcaaatgtca aagtaaagag cacggttcat ttgcctaaag aaggtgttta tgtgtcggaa
    19681 ataaaaattg gggaaacatg gtatccagcc atgggttcaa ttggacataa tgatacattc
    19741 ggtgaaggac gggagctgac tgtagagttg tatattttgg actttaatca agatatctat
    19801 ggtgaacatg tcgcggtacg ctggaatcaa tttttaagag atcaagtgaa attttctagt
    19861 gtagaggaat tgattgaaca actcaaggag gatgaacaag caacagctga atatttttct
    19921 cagcaataaa acattatgca taaaatctct tgtttatgta taactagcaa ttacaataaa
    19981 atgggtgata aataagccca ggaaaggggc tgtgacaaaa gtcttgtcac agcctttttt
    20041 tccaaataaa tggtttttca gaagtagctt cttcggaaat aagccgaaat ttcttgaagc
    20101 tgaccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag caattcatga
    20161 aaacgattgc tagagcgtat catttgcttt agactatata tcttggtgta cactcacttt
    20221 aaggcggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg agtgggtact
    20281 atgtacaagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa ggggattgga
    20341 acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa ctataactcg
    20401 gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg aaaagcagtc
    20461 tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga tacggcttta
    20521 gaaaattttg ggacactgga cgtttgggtt aataatgctg ggatggaaaa tcagcatcca
    20581 acccatgaac ttagccttga agattgggaa agagtgttaa aagtcaattt gactggcgtt
    20641 tttatcggaa cgaaagcggc tttaaattac tttacagaga ataataaaaa aggaaatatc
    20701 atcaatatgt cttctgttca tgaacagatc ccatggccaa cgtttgctca ttacgctgct
    20761 tcaaaaggcg gggtaaaatt atttaccgaa acggtagcaa tggagtatgc accgaaggga
    20821 atcagaatca atagtatagg ccctggtgct atccgtacac ctattaatgc ggcaaaattt
    20881 gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg cgtaggtgaa
    20941 ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag ttatgttaca
    21001 ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca aggtggaaaa
    21061 ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt tggctggggg
    21121 atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat attaggaact
    21181 ggtattggag cggtgcttgt cggcttactc gttcaattat tcgcttctcc agctggtatt
    21241 tcagtgacta catttttgat tagtttactt tctggtgcat tttgggttat tggacaaaca
    21301 ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat ttcgactgca
    21361 ttacagttag tcggaacctc gttgattggt gtcttcgctt ttggagaatg gtctggaagt
    21421 atggggaaaa tcattggagc agcagcaatc attcttcttg tatttggttc tgctttaacg
    21481 gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaattag tctattagca
    21541 tcaacaagta ttggttattg ggtatacagt gcattgccta aactagttga tgcaagcgga
    21601 ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat ttatgtgatt
    21661 ttccgtaaac caaccgcatt ttctgaagac aagagttgga aagcaacgat cgttggagcc
    21721 atctttagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg ggttgctact
    21781 gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat gtacatcctg
    21841 catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt gctgatcgta
    21901 gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaattt catttattaa
    21961 aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct agaatttcca
    22021 acatctctgg aatttctagc gctttttaat tgtttaagat acaagggaaa tatctgtctg
    22081 gatcaataat ttttcattga tccattgact aaagtttacg acataattga agttaccacc
    22141 gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac ggtctttttt
    22201 atttgcgtaa acatcgttat ggtactggac taattgttct cggaattgat tgattgctgt
    22261 aaaatcacga tattgaaggt tgaaacgcat cgtgattggt gcatttagca tgaatcgctg
    22321 catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa gattacgttc
    22381 agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat gaagataatt
    22441 tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg agtaaaaatc
    22501 tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat ttgcttaatc
    22561 acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt cagaaaattc
    22621 taacttttat ttctctaaag aaattcgttc tctcctaaag tgatattata tttaaatata
    22681 cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat tttttcctta
    22741 ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtaga aaattcaaag
    22801 ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat agcagatact
    22861 ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca aaacctgtat
    22921 aaggtgatga atagtttcct cgccagtaaa aggtttattc gtataaagct ttatcgccaa
    22981 aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc aatcaattcc
    23041 cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac gtagcctgct
    23101 tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat agaaaaatga
    23161 tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat aggttggttc
    23221 cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa aaggtctttg
    23281 atcttttgtt gatgttcttt ttctttgatt tgattgatga tcggctgaaa tttttccatt
    23341 ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata gttatgtata
    23401 aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata agatgtctca
    23461 aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat tcttacacga
    23521 aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag atgctttggt
    23581 aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct taaaagataa
    23641 aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt tcaaagcatt
    23701 agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg aagaacaaac
    23761 gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc cgattattat
    23821 tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac cagatcaaac
    23881 gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga tgccgatatt
    23941 tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata cttggaggtg
    24001 aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg ttactgcacc
    24061 atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa agagaatgga
    24121 agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata aggaaaaatc
    24181 aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg aaaatatcca
    24241 aatgatcttt ccgacttggg gaggagaatt attgattgag attctcgatt ttgtcgattt
    24301 tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt tgctacttgc
    24361 tgtcacatta aaaacaggga tagccactgc acatggaact aaccttattg atttaagagg
    24421 agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa gagaaggaga
    24481 atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg tggccccatg agcattcatc
    24541 atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg gagatgcagt
    24601 aaggatgaag ggacgattat taggtggttg tattgatacg atcaggcatt taatcggtac
    24661 tccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc cgatcttgtg
    24721 gtttctagaa aactgtgagc tatcgtcagt tgatttaaga agatcacttg ttcagatgaa
    24781 actggcaggc tggtttgatg gcagctcggg gatcatgttc ggtagaagtg cggcagagga
    24841 tttgttagat tataaaatga aggatgtata cgaagatctg tcaaaggaat taaaactacc
    24901 gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga ttaatggtgc
    24961 ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt ttatttaaaa
    25021 aattttttcc tgccttggag tctatcaaag accaataaat ctaaatagaa aagaaagtga
    25081 aagaagctga caaaatgctg tcagcttctt tttgtataac atattagaaa gtctaattat
    25141 ctataactgt cacatcgatt aactgtttca cgtcattggg atttcctgta ggaatagcta
    25201 ctacagatgc attatcaatg atttcttgag ttgtttctct aatgaacgct ggataagatg
    25261 tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt gtcccgtcat
    25321 ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc ataaccacat
    25381 ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct gcaggataat
    25441 gaataacatg taattcgaaa tcacccgatg tatctctaaa agtattacct ctaatagcta
    25501 gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga tctattgact
    25561 gagaagactg tgaggaggtt gtgtctattg ttgaagaaga aatatttgtc tcaactgtac
    25621 tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa gatgtattcg
    25681 tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt tgtctgattg
    25741 aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac gtacatccta
    25801 caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa aatacctcat
    25861 tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa accttgtcta
    25921 tgaatataaa tagagtgtat cttctttgat aaatgtcaga aaaagtgtga caaataatca
    25981 tcagtaaaat tacttgtcac actattgata aggagtattt aattgaagag aatgattagg
    26041 agaaaggatc tccgtacttt ttatagtaat catagaatcc aagtttgtat agacgaatcg
    26101 ttttttctct tatttcttct gtttcaaaat ggactgcttg aagaatcaag tctgtgaatt
    26161 ctataggagc tgttccattc gcagtaatca aattacgatc tttcacagct tgttgttcta
    26221 taaaatcttc tttattctga taattttccg attctaacca aggaaattgc gcatttccgg
    26281 tgtgtttgta accttgcaat aatccgctct gagctaaata atcaactgcc ccacaaatag
    26341 cagctactgg tacgtctgtt tttaacaatt tatcgattat ttgatataaa tgatgatttt
    26401 ttatgtccca tgaattgcca ccgatcataa taaaaaggtc aactttttca ggtatttgtt
    26461 cgattaagta atcgattttt gtttttaatc ctccaatgga agtgacctct gattggatag
    26521 aagcagtttt gttttgccat ttctcatttt ggtttagtgt gctagtaata taagcacctt
    26581 cccaatcggc atattcctct aatagaaacg taatcgcagt tttcattttc aagctctcct
    26641 ttactatcta taacaaggac aacagtttta acctacagac attcattaaa attgggtagc
    26701 gacagcgaaa acaacaattc ttaagtttta gacagagtat acagatagtc aacttaatta
    26761 aatacgaact aaataaaaat gacaggtaat catttttgtt taccagcatt ataacatttt
    26821 gcatacatgg atgacaaaag aaccaattcc cacttgcttg agtacaatat taaaagaaac
    26881 tggaaggcaa aaagtaatta tagaaacgaa aaataaatca tatcgggcac tcagacatga
    26941 aaacaaccct agagatatat aactaggtaa ccagtttaat gaaagaaaaa atcatccaag
    27001 aagtcgattc ttggattttt tagtggattt gttcgccaaa tacgtttagt atgcgtatat
    27061 gagaggttgt aagcaaagcg ttgtggtctg agcacttaag gaggaatctg tgaaaatagc
    27121 ttttcatatt tttgcagatt ttgacttaat gccgaaggtc agctttgcga ttaccgttta
    27181 tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat aagcaagcat ttttagagct
    27241 aggtttcctt gattctaaat atgcggctta tttcaatgag ttggtgcttg aacgcgattt
    27301 taagaaggtc gaaaacaggc acgacttttc gacgtaagga gaaagaattt caatgataga
    27361 aacaataaat cccgcagaaa acaaggtttc tgcgtacatt catatcccat tttgcgagca
    27421 tatctgctat tactgtgatt ttaataaagt ttttttagaa gggcagccag tagatgagta
    27481 tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa agtcatccga cagcgaattt
    27541 agaaacactt tacgtagggg gcgggacacc aacttcttta tctgctaaac aacttgatcg
    27601 actgttatca ggtgctagag agatcctgcc ttttcaaaat ggtaatgagt ttactgtaga
    27661 agcgaatcca ggtgatttga cccgtgaaaa acttcaagta atgaaaaact atggcgtcaa
    27721 tcgtttatcc atgggagtgc agacatttga taatcgctta ttgaaaaaaa ttggacgtaa
    27781 acacaccgca gaagatgtgt atcaaactat gcaattttta gaagcagaaa attttaccaa
    27841 tgtaagcatc gatttgattt atgcattacc gggacaaaca ctagaaggat atctggatac
    27901 tttggaaaaa gctttggctt tggatctgcc tcactattct ttgtactcat tgatcctcga
    27961 aaacaaaaca atgttcatga actgggtacg acaaggaaga ctcgaactgc ctgatcagga
    28021 aacggaaacc agaatgtttg aagagacgat tgaagcaatg gaaaagcatg gacgtcacca
    28081 atatgaaata agtaactttg ggctctccgg acatgaatct caacataacc tcatgtactg
    28141 gaacaatgcc cactattttg ggtttggagc tggtgctagc ggttatcttg gagatattcg
    28201 ttaccgcaac aaaggaccaa tccaacacta tttacgccca ttacgagcaa atgaacgccc
    28261 ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata gaagaagaaa tgttcttagg
    28321 actaagaaaa aaaacaggga ttactaaaaa acatttctat gatcgctatc aaaaaacaat
    28381 tgaatcggtg tacggtgatg tgctaaggga attagagcaa gaagggttac ttgttaatga
    28441 agaagaccgt gtttatttga caccaagagg gacctttttg gggaacaatg tgtttgagcg
    28501 ctttttgttt gaaaaatagt ttaaagaata tttgtttttt atctgaattt aaataaaaaa
    28561 agttcaaaaa tacaataaag gactattgaa acgttgatat aactggattt acatcaatta
    28621 tttagaatta gcactcttgt gtttagagtg ctaaaaaatg gagtctttct cttgacagga
    28681 gaagggcttc ttgctatatt tataagtgga ttagcactta gaatatatga gtgctaatga
    28741 gaggtgagga agatgttaac aaagcgacaa aacgacattt tacgtctgat catccaacac
    28801 tatacgagta gtggcgtgcc cgttggttcc aaaacattga tgtctgaggg agtcaaggcc
    28861 agctcagcaa cgattcgcaa tgatatgaaa gcattagaag atgaaggatt gctgttgaag
    28921 actcactctt cttctgggcg gattccatct gctttaggct atcgttatta cgtggatcat
    28981 ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc tgattcgtca gtctctaggc
    29041 agagaatttc atgaaatcaa tgagattatt aagcaatctg ctgagatatt atctgagctg
    29101 acaagctata cgactttttc attgggacct gaaataaaag accgccgact gactggcttt
    29161 cgtatcgtac ccttgaataa tcgacagatt attgctatcg tcgtaacaga tatggggaat
    29221 gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa gtcaggaact agaaaaaatg
    29281 gtacagattg taaacgaccg tctagtcgga gatcctctga tgacggtgta ccataaactt
    29341 cgtacggaga ttccgatgat tcttcataaa tatttccaaa cgacagaagg gatgtctcag
    29401 ttatttgaca ccgttcttgg tcatgttttt gaagataagg tttatgtcag tggacaaatg
    29461 aatctcttgg attatgagcc ccgtcaagat cttgatcagt tcaaatcgat gttttctttt
    29521 atgaaagact cagacggact tacacaaatg atcactccga tggatagcaa tattcatatc
    29581 aagattggtg ctgaattagg caatgattta ttgcagaata tgagtatgat tcaagcaagt
    29641 tacgagatag atggtcatgg aagaggtaca atagccttgt tagggcctac tagtatgcct
    29701 tattctcgga tgttcggtct agtcgatgtt tatcgaagag aactagcagc taaacttgct
    29761 gattactacc gttcgcttga cttgtcaaac tcgtaatgtg aataacgaag ctatttgcag
    29821 ctgatcgttc tctctttctt agtaagagca atcaaacagc tgactgaata atgaaaggaa
    29881 gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag aaatgacaga tgctcagcca
    29941 gaaccggaaa tcgatgtcga agctgcagaa gattcgggta tttcagaagc tgaagcggaa
    30001 gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa tggaagataa atacttgcgg
    30061 gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa atgagcgtga gcaattacaa
    30121 aaataccgtt cacaagattt agccaaaaaa ttacttccat caattgacaa cctggaacgt
    30181 gcattagcca cagaagtgtc agatgatcaa ggggcaggtt tgaaaaaagg cgtggaaatg
    30241 gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa ttgaaaaaat ccctgctaaa
    30301 ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa ctgttccagc gactgaagat
    30361 acaccagccg atacgattgt agaagtacta caagaaggct acaaattaca tgatcgcgtg
    30421 ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg gatataaaac ttacactttc
    30481 aattaaagaa ggagacgtta attatgagta aaattatcgg tattgactta ggtacgacta
    30541 actctgcagt agcagtattg gaaggaaacg aagcaaaaat catcgctaac ccagaaggaa
    30601 accgtacaac accttctgtt gtatcattca aaaatggaga aatccaagta ggggaagttg
    30661 caaaacgtca agctgtgaca aacccaaata cgatttcatc aatcaaacgc catatcggtg
    30721 aagcaggtta caaagtagat gtagaaggta aatcatatac accacaagaa atctcagcaa
    30781 tgatccttca atatatcaaa ggattcgcag aagactattt aggtgaaaaa gtcgaaaaag
    30841 cagttatcac tgtgccagct tactttaatg atgcacaacg tcaagcaaca aaagatgctg
    30901 gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga accaactgca gctgctcttg
    30961 cctatggttt agataaaaca gatcgcgacg aaaaaatctt agtattcgac cttggtggcg
    31021 gtacttttga cgtatctatc cttgaactag gagacggtgt ctttgatgta ttgtcaacag
    31081 ctggcgacaa ccatctaggt ggggacgact ttgataacaa aatcatcgac tacatggtag
    31141 cagaattcaa aaaagaaaac agcattgact tgtctcaaga caaaatggct ttacaacgct
    31201 tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg cgtatcaagc acacaaatca
    31261 gcttgccatt tatcacagca ggagaatctg gaccattgca tttggaaatg acattgacac
    31321 gtgcaaaatt tgatgaatta acagctgact tagtagaacg tacaaaagta ccagtacgtc
    31381 aagcgctaaa agatgcaggt ttatcacaat cagaaatcga cgaagttatt ttagtcggtg
    31441 gatctactcg tatccctgca gttgtagaag cagtacgtaa agaaacagga aaagaaccaa
    31501 acaaatcagt taaccctgat gaagtagttg ctatgggtgc tgctatccaa ggtggcgtga
    31561 tcactggtga tgtgaaagat gttgttttac ttgacgttac accattgtca ttaggtatcg
    31621 aaacaatggg tggagtgttc actaaattga ttgaccgcaa tacaacgatt ccaacaagta
    31681 aatcacaagt attctcaact gcggcagata atcaaccagc agtagacatc catgtattac
    31741 aaggtgaacg cccaatggct gctgacaaca aaactctagg aagattccaa ttgacagata
    31801 ttccagcagc tcctcgtggt attccacaaa tcgaagtaac attcgatatt gataaaaacg
    31861 ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa agaacaaaaa atcacgatca
    31921 aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat ggtaaaagat gcagaagcaa
    31981 atgcagaagc agataaagct cgtaaagagg aagttgacct acgtaatgat gtagatgcat
    32041 tgctattctc tgtggacaaa acattgaaag aattagaagg caaagtggac gaagaagaag
    32101 tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc tgttgaagca aacaacatcg
    32161 aagaaatgaa aactaaacgc gatgcattga acgaaatcgt tcaaaacttg actgttaaac
    32221 tatacgaaca agcggcacaa caacaagctc aagaaaaccc agaagcagct caaggcggag
    32281 cagatgatgt cgttgatgct gattttgaag aagtcgatgg cgacgataaa taatcagttc
    32341 actgaatttc gagagaaggc caaagttgct actttggctt tttcttttga aaaatagttt
    32401 gaagtatcgg tttttgtttc aaacaaaata tgatatgata acaagcgacg tgaaaatgat
    32461 gagaaccaat aggaggaaag cctatggcaa caaaacgtga ttattatgaa gtcttagggc
    32521 tctcaaaagg agcatcagaa gatgaaataa agaaagctta tcgtaagctt tcgaaaaaat
    32581 accatcctga catcaataaa gaacctgatg cggaagaaaa attcaaagaa gtatcagaag
    32641 catatgagat tttaagtgac ccgcaaaaac gcgctgcata tgaccaatac gggcatgcag
    32701 gcacagatcc aaattacggt gctggtggag ctggtggcgg atttggtgga ttcggcggct
    32761 tctctggtgg cggcttcggt ggttttgaag atatcttcga ctctttcttt ggaggaggcg
    32821 gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga tttgcaatat acggtcgact
    32881 tgtcttttga agaagcgatt tttggtgtag aaaaagaaat caagtataat cgggaagaaa
    32941 tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac acaaccaact acttgtcata
    33001 aatgtcatgg tgcaggtacg attaatgtag aacgccaaac accacttgga cgagtgatga
    33061 gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga aatcaaagaa ccttgtccaa
    33121 cctgtcatgg ctcaggacat gagaagaaag cacattcagt taaagtgaat gtaccagctg
    33181 gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg agaagctgga gaaaacggcg
    33241 gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga aagtgatatc tttgatcgtg
    33301 aaggttctga aatttactat gaattgcctt taagttttgt acaagcagct ttgggggatg
    33361 aagtccaagt accaactgtc catggagatg tgaaattgaa gatccctgct ggaacacaga
    33421 caggtacgaa cttccgttta cgtggaaaag gcgctccgaa gttacgtggt ggtgcaacag
    33481 gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa tctaaatgaa caacaaagag
    33541 aagcattacg tgcattcgct gaagctggcg gcatcaaagt tgaagaacag caagaagacg
    33601 gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa gcggaagtag tttttctagt
    33661 aaaaacgcta agtcattaat aattaaaaac cagacgaaca atcatatgtt cgcctggttt
    33721 ttttgtagtt gtcatgtttg taccaacaaa aaagccagct ttagaatatg ataatcgtca
    33781 tagtttctag gggtataacc agtgagagaa tggaacttgt tcaatcgata ttgcaaggtg
    33841 tttttatgaa taaataattc ttctgagcaa tttttgatgc ttccgttatt tttactgaag
    33901 catgccaaag tcttcttgaa caaatgtact tcttcttctg tcaggctttt caaaatacgt
    33961 tttacatata atgttcgttg atctgacggg attgctggta aaacgatccc gaggtctaaa
    34021 tcttcaaaaa agcggatctc gttcacagat tgatggaaag ctatccattc gcaaacttgt
    34081 ttggcatgaa ggaaagattg cggtgtctct tgctctgtat aacaaagcga cccaattcca
    34141 aagtttagct gtaactgata cattttatta aactttgtct ggattttatc aagcaatgat
    34201 agcaatgagg ctctggaaat ccgattgact aggataatga tttgttgtct atgtacagtg
    34261 agcaaagcct gtccgtaata agagaagacc ctttcgagca actccaataa aggattgatc
    34321 cgttcttgac acaattgctg attgtcagaa agttgtccca caactacata atatggagaa
    34381 gaaaaatcaa gctcaattaa gtgagggtgg tatcgactat gtacttgata tcatcaatga
    34441 accgatggta ctttcttatg cgatggaaca ggtagagcga ttgcttgttt gtgcaggaaa
    34501 acaagcgatg cggctgatac gattaggtga gaaaataaga agataaaaat acacggtccc
    34561 ttcatggcga ccgtgcattt ttggttttta ttaaatattt aatactttat tcaagaaatc
    34621 ttttgtccgt tcattttgcg gattttcaaa gatttgttga ggcgttccat cttctaaaaa
    34681 gtttccgtcg tcaatgaaca tgacgcgatt ggccacttct ttagcgaacc ccatttcgtg
    34741 cgtaacgata accatcgtca taccttgttc tgctaacttt ttcatgacgt tcaagacatc
    34801 accgaccatt tcaggatcga gtgcagaagt aggctcatca aaaagcatga tgtcagggtt
    34861 catcgctaga gcacgagcaa tagccacacg ctgtttttgt ccaccagaaa gcatttcagg
    34921 atacatatct tttttatctt ctagaccaac tgtctgcagt aatttgattg cttttttctc
    34981 tgcttcttct ctagattctt tcttcaggtc catcggtgct aaaatgatgt tttccaaaat
    35041 agaaagatga gggaataggt taaagtgttg gaataccatt ccgatatgtt gtctcaccaa
    35101 gttgatatct gtgcttttat ccatcaagtg ggcgccgtca ataataattt caccgtcaga
    35161 aggttcttct aaacgattca agcaacgcaa gaatgtactt tttcctgaac ctgaagggcc
    35221 aatgatacag acgacatctc cttcgttgat ggatacattg atgtcgttta atactgtgtt
    35281 atcgccgtat tttttgacta aatgctcaac taagattttt tctgccatct tacttcacct
    35341 tcttttctaa aacttttgct aattttgtca atgctgtgat caaaatcaag tacatgatcg
    35401 caatgatcaa gtaaacatac gtactttgtg ttgtacgtgc aacaatgatc ttaccagttt
    35461 gcagcaattc gactacaccg attgcggaaa tgatcgttgt gtcttttaat gagataacga
    35521 actgattgac gaatgatggg atcatgatgc gtatcgcttg cggcaaaatg attttttgca
    35581 ttgtccgatt ataagaaaga cctaaacttc tggaagcttc catttgtccg acaggaacgg
    35641 cgttgatccc gcctcgaaca atctctgcga tataagcact tgcgttaaga gtcaaggtga
    35701 taacaccagc aaggaagtca ggaatcgtaa atccaacgat accaggcaac ccaaagaaaa
    35761 taaagaacgc aagaaccatc attggaattc ctcggatgat atcgacataa atactagcaa
    35821 ttgtgcgaag agcgcgtatt ggtgctacac tgaataatcc aaaaatcaca ccgattacta
    35881 tagccaaagc aaaggagatc aaagttagga gaattgttct ccaaagtcct ttcaacaatg
    35941 cttgatagtt gttttggata agtccaagaa atgttgattc atcagcagtg gaagactcac
    36001 tggaagtttc tgaaccatcc gctacatatt ctttaataat cttatcgtat tcgcctgttc
    36061 tttttaattc tttcaatcct tcattgaaca tattaagaag ctcaggattt tgtccttttt
    36121 taacggcaaa tgcatatttt ccgccagatt cttttttgat cggtgtttcc aattcttgtc
    36181 cttgtttgat cgcataaccg attaccggat aatcatccat cattgcatcg attgcgccaa
    36241 cttttaaccc gtcgtacaat tgatcagcag catcaaaata tttgatagaa tagccatatt
    36301 cagattcatg gtcgcttaaa aattcagcac tttcggtacc aacttttgct ccgactgttt
    36361 ttcctttcaa atcttcataa gacttgatcg aagtatctcc ttttttgaca gctaattgga
    36421 tcccgctatc aaagtaagaa tcagaaaaat catagctttt ctttcgttca tctgttacag
    36481 tcattccggc aatcattccg tctgcttgtc ctgattccaa agcttgcaaa gcagcagaga
    36541 aaccacggta gtccattcgg atgttaaatc cttgcagttt ggcgatgttt tccatcaatt
    36601 ttacatcgat gccttcgtac tcgccctttg cgttttgaaa ttcaaatgga gcaaaagcag
    36661 agtcactggc aattacgtat acgtcttttt tgggctctat tttttgcatt gtctcgcttg
    36721 tgcttgactc tgccgttgta gtgttgtcag cttgtgcaac atgaccaaac gcaagtgtca
    36781 caagtcctgt cattaagaaa acaaatgagt gaaataaatg ctttcttttc ataatcaacc
    36841 tcctaaattt ccaata
//
LOCUS       DAYYAR010000012        36612 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000012 DAYYAR010000000
VERSION     DAYYAR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.012"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            418..738
                     /locus_tag="ACHAT0_000518"
     CDS             418..738
                     /locus_tag="ACHAT0_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8450487.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            892..1797
                     /locus_tag="ACHAT0_000519"
     CDS             892..1797
                     /locus_tag="ACHAT0_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450488.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1794..2567
                     /locus_tag="ACHAT0_000520"
     CDS             1794..2567
                     /locus_tag="ACHAT0_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8450489.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2748..4172)
                     /locus_tag="ACHAT0_000521"
     CDS             complement(2748..4172)
                     /locus_tag="ACHAT0_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8450490.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4383..4829
                     /gene="nrdI"
                     /locus_tag="ACHAT0_000522"
     CDS             4383..4829
                     /gene="nrdI"
                     /locus_tag="ACHAT0_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8450491.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4894..6876)
                     /locus_tag="ACHAT0_000523"
     CDS             complement(4894..6876)
                     /locus_tag="ACHAT0_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8450492.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6890..7786)
                     /gene="murQ"
                     /locus_tag="ACHAT0_000524"
     CDS             complement(6890..7786)
                     /gene="murQ"
                     /locus_tag="ACHAT0_000524"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8450493.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7789..8850)
                     /locus_tag="ACHAT0_000525"
     CDS             complement(7789..8850)
                     /locus_tag="ACHAT0_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8450494.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(9061..9588)
                     /locus_tag="ACHAT0_000526"
     CDS             complement(9061..9588)
                     /locus_tag="ACHAT0_000526"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8450495.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9655..11157)
                     /gene="eat(A)"
                     /locus_tag="ACHAT0_000527"
     CDS             complement(9655..11157)
                     /gene="eat(A)"
                     /locus_tag="ACHAT0_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8450496.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11415..12536)
                     /locus_tag="ACHAT0_000528"
     CDS             complement(11415..12536)
                     /locus_tag="ACHAT0_000528"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8450497.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12662..13603
                     /locus_tag="ACHAT0_000529"
     CDS             12662..13603
                     /locus_tag="ACHAT0_000529"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8450498.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13753..14649)
                     /locus_tag="ACHAT0_000530"
     CDS             complement(13753..14649)
                     /locus_tag="ACHAT0_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8450499.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14918..16258
                     /locus_tag="ACHAT0_000531"
     CDS             14918..16258
                     /locus_tag="ACHAT0_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8450500.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16476..16919
                     /locus_tag="ACHAT0_000532"
     CDS             16476..16919
                     /locus_tag="ACHAT0_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8450501.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17423..18841)
                     /gene="nhaC"
                     /locus_tag="ACHAT0_000533"
     CDS             complement(17423..18841)
                     /gene="nhaC"
                     /locus_tag="ACHAT0_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8450502.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19244..20467)
                     /locus_tag="ACHAT0_000534"
     CDS             complement(19244..20467)
                     /locus_tag="ACHAT0_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8450503.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20498..21337)
                     /locus_tag="ACHAT0_000535"
     CDS             complement(20498..21337)
                     /locus_tag="ACHAT0_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8450504.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21354..22034)
                     /locus_tag="ACHAT0_000536"
     CDS             complement(21354..22034)
                     /locus_tag="ACHAT0_000536"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8450505.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(22036..23109)
                     /locus_tag="ACHAT0_000537"
     CDS             complement(22036..23109)
                     /locus_tag="ACHAT0_000537"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8450506.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23165..23403)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23446..23679)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23756..24427)
                     /locus_tag="ACHAT0_000538"
     CDS             complement(23756..24427)
                     /locus_tag="ACHAT0_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8450507.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24514..27162)
                     /gene="mgtA"
                     /locus_tag="ACHAT0_000539"
     CDS             complement(24514..27162)
                     /gene="mgtA"
                     /locus_tag="ACHAT0_000539"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8450508.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27504..27678)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27851..28288
                     /gene="ndk"
                     /locus_tag="ACHAT0_000540"
     CDS             27851..28288
                     /gene="ndk"
                     /locus_tag="ACHAT0_000540"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8450509.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28310..29176)
                     /locus_tag="ACHAT0_000541"
     CDS             complement(28310..29176)
                     /locus_tag="ACHAT0_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8450510.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29336..31429)
                     /locus_tag="ACHAT0_000542"
     CDS             complement(29336..31429)
                     /locus_tag="ACHAT0_000542"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8450511.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32178..32801)
                     /locus_tag="ACHAT0_000543"
     CDS             complement(32178..32801)
                     /locus_tag="ACHAT0_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450512.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32960..34357)
                     /locus_tag="ACHAT0_000544"
     CDS             complement(32960..34357)
                     /locus_tag="ACHAT0_000544"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8450513.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34389..35360)
                     /locus_tag="ACHAT0_000545"
     CDS             complement(34389..35360)
                     /locus_tag="ACHAT0_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8450514.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35734..36159
                     /locus_tag="ACHAT0_000546"
     CDS             35734..36159
                     /locus_tag="ACHAT0_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450515.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        10600 a         7618 c         6405 g        11989 t
ORIGIN      
        1 aaatatagtt gttttctcac gaaatagaca ctatttttaa atatttttcg ttattttttt
       61 atcacaaaaa ttatcttact tattgtaagg taaagctttt tttaatttat tgcccgataa
      121 tatatagaaa taaggtctag ttactgtttt ttttaaatga ttaataaata acaacttctt
      181 tctatgaaat tgcaaagcat tcatcttctt ttccgctaac gatatatccg tcagtgcttg
      241 gtacacttta ctaaaaagta tgagattgcc gatatctgga taggtgtgct atactacaga
      301 aaaagatata caatctgcca ccgggtagga taaagaggat gatcattcca ttaggtcttt
      361 gaagggatga ttttcctctt tttgtgtatt tttttatagt taaggaggaa tgtaagtatg
      421 gcatggatgg aattaatcat agcaggaatt ttggaagtat tctggtcaac gatgatgaaa
      481 tggagtgatg gtttttctaa aatcaattat tctttttaca cagtgattgg catgattgct
      541 agtttttact ttttgtcaaa ggcaatcaaa agtttgccga tgagtctctc ttatcctatc
      601 tggacaggga ttggcgcagt tggttcggtc ataattggtg tggtcttgtt tcatgataag
      661 ctgaatcttt ccacttggtt ctttgtgggg atgctactca tcagtatcat tggcatcaag
      721 attactagcg gccactagac acttggaata agtactgatc agaaaaaata agccttttta
      781 tttaatattc aattttttta tgaaaaaata agaaataagt ccaatgacct agcgacttct
      841 ccactcatca ggtgtatgct tgtactatct atagaaaagg agtgaatatt tttgaaaagt
      901 ctttcggtcc aagaattatc caagaacttt ggtcacaaac aagccctcag cgatgtcagt
      961 tttagcttat cccctggcaa aatcgttgga ttagtaggtc ctaatggcgc tggaaaaaca
     1021 acgatcatga aagctatttt aggactgatc cattactcag atggaacaat ctgtattggt
     1081 gaaaatgaga tttctccctc tagtcataaa gggttggaac aagtaggagc attgatcgaa
     1141 tacccgggta tctatccatt cttaacaggc tatgatcatc tgaagttatt ctctgaaacc
     1201 aacgatgtat ctgctatcga cactatcgtg aatcagctca agatggaaaa gtacattcac
     1261 aaaaaagcga aatcctactc gttgggaatg aaacaaaaat tgggaattgc tcttgcctta
     1321 ctgaaccatc cgtctatcgt tattttagat gagccgatga acggtttgga tccacaagca
     1381 acaagagatg tgcgaaaaat cattaccgac ttggctactc agggcacctc tttcttgatc
     1441 tccagtcact tactcagcga gcttgaaaaa atggtcgatg agctgatcct gatcaatcaa
     1501 ggaaagatca tcaaacaatg tacaatggct gaattgaacc aaacagcgca agatttcatc
     1561 gttatctcta caacaaatga tctagctgct cgaacgatct tgacagaagc tggatatact
     1621 ttggaagatc ctgataaaat caagctttta aaagaaaacg aagaattatt aggaaccgtg
     1681 atccagctat tgtctgcaca aaatatccat gtgacagacg tccagcatct gacgaatgat
     1741 ttggaatctt ctctattgga aattctggat tcagtaaagg gggaagaaga aaaatgacat
     1801 tgcttgtaaa acaagaatta tttaaattga tcaagaaaaa gtcaactgct gttttatctg
     1861 ttctattagt tgttttgctc ataggaacag cacttctagc caaaaaatac acgacgatca
     1921 ttgatccagt tgaaatgact gctcagcttt ttagtgccac atcttggatt gtctttatca
     1981 tgattgcagc tgcaagcacg atcatttcga tggaagcaca atatggtaca ttgaaaaatc
     2041 tcctttatcg aaagtattct cgaggagaaa tattagtaag caaatggatc acattagtta
     2101 tttattctgt ttatttatac cttttagcta ttattgtcac tgtgttgatg aaactgattc
     2161 ttttcccttc gatttccttc acggagaagg tttctactgg tcaaacactg atccaaagct
     2221 tgttcactta tactttgggt agttatatcg gtttatggct gattttaagt ttagtgttga
     2281 tgattgcttg ctttatcaat agttccggcg catccatctc agcaggaatc gtcttttatt
     2341 ttgcttcgtc aatcatatct ggtatcctta ttgcactcat ccaaaaatgg gagtggatca
     2401 aatggaatcc gatcagtatg ttgaatctgc aaaaccaaat tggaaacgaa gagatcatga
     2461 agcaattgac tcatctttct actaatcaaa tgctattcgg taatttagct tacatcgttt
     2521 tgttcttagc attaggatat ctcgtattta agcgaaaaaa tgtatgatga ctcatcaaaa
     2581 aagaggctgt gaaagagacg ttcagcctta ttgccgattg gctgtctctt aaacaccgtt
     2641 tattctgaaa aaggagctgt gacaacattt gtgtcacagc tcctttccta gtatgttttt
     2701 gaataaacga caggctcaca acctctcttt atacttcttt tatacttcta tacgtgaagc
     2761 atcccagtgg caaataaaac cattgcaatc actccgacca tcgtcacacc tataagtaaa
     2821 atcttatcta agtggatatg ttgtacaggc tcgttttgct ccttacgtgc tttgaggtaa
     2881 aaataaatgc ctggcaagaa aagaacaaga acaagcatta cttcattaaa ggctacgact
     2941 agcatagccc atacttgaaa ggcacttgct aaaagcccta ctaaaaattg gacccattct
     3001 ttccgctgat aagaaagttt catttggtac aatcctaaga aaagccacgt gaagaaaata
     3061 gctgctgtac caagtgtgta agcaaagtta taagcactgt cagtgaataa taatgaaagt
     3121 aaaaagacat tcgtacataa cgtcgtcaat acgatagcat aagttggtgc cccatttttg
     3181 ttgagttttc cccatgcttt aggcaaaagt ccgtcttcag ccatgtttcc cattgtttca
     3241 atcggtaaga tcgtccatga gagccaaaca ccaactaaag aaatgatcaa accgccgttg
     3301 atcacgactg ctccccattt accaaccatc atttcaaaca catagcccat agcaggctgc
     3361 gccttgatcg tttgcagctg attcatcgtc attgtcccat atggaagcat ggaaaccaag
     3421 acgtaaataa taatcaagcc gaatatccct agaatcgttg ctttcccaac atctgattgt
     3481 ttttttgctc gtgaacctac gacagaggct ccttcaatac caacaaatac ccaaagcatc
     3541 accatgatgc aactcaatac ttgactggaa aacgggatcg gctgtccatt cgcattcaat
     3601 ataatgtcgc tagaaaagtt atcaatgaat tgttggatcg aaaacgtgct gatagctact
     3661 acaataaata aaaataatgg cactaattta cagaatgtga cgatagcatt gacgaaacta
     3721 gcattttcga ctccccgatt cactagccat gcatatcccc ataaaaagac tgtactcatc
     3781 acgatcgatg ccagattttg cccaccttca aatactggga agaaatatcc taatgcgctc
     3841 atcaacaatg ttgcaaaagc tacgttgcct aaccagcatg tgagccagta accccaaccg
     3901 ctgataaaac cagcgaaacg gccaaaacct tcttctgcgt aactaaagat cccgcctttt
     3961 aaatctggac gttttttatt gatattattg atcgacaagg agagcatcaa cattgctgtt
     4021 cctacgagta cccaactgaa aatagctggg ccgccagccg atccggagat gtccgtcata
     4081 ataccaaata cgccacctcc aatcgctgaa ccaattacta aggcagcaag tggaattaaa
     4141 ccaattcctt ttttctgttg ctctgtatcc atgtaagcat cctccaaaat aaaaccgctt
     4201 ttatcaaaaa gtataacaaa ccttttttat ttttacttaa ttaataaaaa ctttaataga
     4261 aatataattc taccggcttt tttctcatta tacaagcaaa gcgcctagtc cctttccaaa
     4321 ttacaccacc aaaaaactta aaaaagatgc tatactaaaa ggaaagaatt ggaggatgat
     4381 ccatgaacat tttatatata tcgatttcag gaaatacgcg tgcctttgcc aaacatctag
     4441 cagaatatgc tgaaaaaatg catgcagaag atcctgtcaa tcctgaagtt actttaaaag
     4501 aaatccatga aaatagtgat tttacgaaag aaacagaacc attttttact ttcgttccta
     4561 cttatttaga cggcggaaat ggtttagata acggtgatac agaaatcttg accgaaacga
     4621 tgcgtgaata tctggaacat gaagacaatc ataaactttg tcttggtgta gtcggtagcg
     4681 gaaataaaaa cttcaataat cagtattgtc taacagccaa acaatatgca caaacatttg
     4741 gctttccttt cttggcagat tatgaattac ggggaacacc atctgatgtg acacgaattt
     4801 atcagatttt agcagaaaac aataaataat aaaaaagaag aagctgaaaa atagatttca
     4861 gtttcttctt tcgtttctat tgtttctatc atattaagct ttgacatcca tcacaggtgc
     4921 ttttgctttt gctttacctg ttttttccaa gacaaagttt tttacacgat ccatattggt
     4981 taccaagacg atcatggatg tttctttgcc tgctgctttg atcgcttcta agtcagcttc
     5041 tgctacaagc gtatcaggtg tcacttgctg cccttctgtt actttcacat caaatggcgt
     5101 accgcctaag tcaactgtgt tgattcccat atgaagcaag atttctaaac catttggcgt
     5161 tgtgatccca atagcgtgtt tcgttgggaa gacagagctg actaccccag taactggaga
     5221 atagatatgt ccgttttctg gttctacaaa gtagccttca cccatcatct tttgtgcaaa
     5281 aactggatca cttgctttat ctaaagaaac aactgttccg tcagcaactg atgagaggat
     5341 agtcacactt tcagctgaag tttgaggtac tactttttca ggcacttcat ttactgtggc
     5401 tgtttcagtc gctaattctt ctttttctaa acggtcttta ggaataccga agaagaatgt
     5461 agcaacgaag ccaccagcat aagctgccaa taatccaagg atgtagccta accatttacc
     5521 gtcagagatc aatgggatca aagcggctcc tgaaggtcca atagcgatag ccccgacatt
     5581 tccgatcatt ccgatcactg ccccgccgat acctccaccg atacaagcag taataaatgg
     5641 gcgtcctaga ggtaatgtta ctccgtaaat caatggttcg ccgattccta ggatcccgac
     5701 tggtaaagct cctttgatca tttctgttaa ttctttatct tttttgcagc gaatccataa
     5761 tgccaatgct gcacccactt gtcctgcacc agccatcgca agaattggaa gcaatagcgt
     5821 acgaccagtt tcatcaatca tttgcacatg gattggagtc aaaatttgat gcagtccgaa
     5881 cataaccatc ggcaagaata atgtgcctag aacgaagccg gcaaatgctc caccaacgcc
     5941 aagtacccag ttgatcccac cgaccatgcc atcagaaatg aaacctgcta aaggcatgat
     6001 gaagaaaatt tcaatcagac cgatggcaat caatgaaaga gttggtgtaa cgatgatatc
     6061 taccgcatca ggtactactt tatgcagttt tttctcaacg attgacaata gccaaacagc
     6121 gaagatgaca cctaggatcc caccttgtcc agctgcaaga gcttcaccag taaataagtt
     6181 tttcagtggt gcttccggat tcatccctgt cagcatcaca acggccccga taactccacc
     6241 aagagttggt gttgcaccaa aaacttgtgc agcattgata cctgtataga taactaggta
     6301 gctaaacatc ccgtttttca agatgttcat cacatcgata tattgttgcc atgttgctgc
     6361 atcaagtgtt ccggctgtga ctaagtttgc taaaactgcg gcaatccctg ccacgatccc
     6421 agtaccgaca aatgcaggaa tcattggcac aaagatatta gaaatgtctt ttaataccga
     6481 tttgatttta gatggttttt gtttcgattt ttgtgccgct ttcatttctt gtgcccgttc
     6541 gtttacacgg tcttttccag atgctgatga atttgctgta gctgtactta cagaagaaag
     6601 tttttctcct aatttcacgc ctgccaaatc aaccatttgc tgtgctactt tgttcacttt
     6661 cccaggtccg atgatgactt gaagctgttc atcttctact acaccaagca ctcctggaat
     6721 cgcttttaat tcagagacat ttacaagatc atcattgatg atcttcatac gcacacgagt
     6781 catgcagtgg acaatgcttt ctacattgcc catgccgcct gcccctttat aaatttgttt
     6841 tgccagttct tccaaagtca gttcgttttt tgccatggtc ttttccccct cataaggtcc
     6901 cttttacaaa tccgtttgct tcagctaatt ttgcttgagc ttcctcttta tcactatttg
     6961 tcaaaatcat gacgatagcc agcttaacat tttgatctgc ttcttcaaag tacttttcag
     7021 ctgtttcgta atcagcttct gtcgcttgca tgatgattcg ttttgagcgt tcgattagtt
     7081 tttcatttgt tggtttgaca tcgaccatca agttattgaa tactttacct ataccaatca
     7141 tactgatagt agaaatcata ttcaagataa gtttttgtgc tgttcctgac ttcaatcgag
     7201 tagagcctgt caaaaattct ggacctgcat cgacttcgat tgggaaatcc gcgtgtttac
     7261 tgatttcggc ttgtttattg caagaaatcg ttcctgtgac agcaccgatc gattttgcgt
     7321 aatctaatcc accgatcaca tatggtgtac gaccgctagc tgcgatacca ataactatat
     7381 cattttttgt caaattcagc ttgattagat cttcttgccc tagctcataa gaatcttcgg
     7441 ccccttcgac agctacagtc attgcttctt ctccaccagc aattagacct atgaccattt
     7501 ccggttctac accgaatgtc ggtacgcatt cagcagcatc taacacacct agtcttccac
     7561 ttgttccggc tcccatataa atcagacgtc cacctttttt aaaggactca atcgtgttag
     7621 caatcactgg ttcgatactt gtcagttctt tttcaaccgc agcggccact ttctgatctt
     7681 ctttgttcat tagttgaacg gcttgagaaa tactcatctc atctaatcca aaagtttctt
     7741 ggtttctgcg ttccgttgtt aggttctcta aattgatttt gctcattttt atcttccttc
     7801 ttttcttata aattgatagt tcataccagc tttgatttgg ttgatcaatg gtagctcttc
     7861 tttgataact ttagctacta cattcacttt ttcatccgct ggcaaatcat atttggttaa
     7921 ttggatctca cccatatagc gcaaatactt ttcgttatct aaagtaatag accctttcat
     7981 acgcgtagct gtatttcttg ctgggatagg cggaatctct cgaaaacgtg catctacgct
     8041 tcgtatgaca tctcttgcat cgtcttgccg attcgtatgc ttccctaata tatattcgta
     8101 aaattcttca tctacaggtt ccgtatggag caagactgtc tgttcttttt ggaaagaagc
     8161 aaattgctcc tgaacgtttt ctgacagacc tgcatctccg atgtaaacaa tatccgtgtt
     8221 acttgctaat agatctagag cgcctgcaag aggatggatc cctcgatgtt cttccaaagt
     8281 tggcaatcct tgatatagtg ggcctctcaa ctctgtatct cctggtacaa aaccttggac
     8341 aaggagacct tgaattttga gccattgatt ttttttctga taccattctt tgtccagccc
     8401 tgtttcggga cgtggataat aattatgcca agcttcaatc tgtgagaaat cagcttctgc
     8461 ttctttcaat tcatcgatat ctttaggcgt gatcgtgcta gcattcaaac tgattttcaa
     8521 ttgatgtgac cagtcggcaa tctgctgatt ggaaatatga taatccattc ttaaacctgt
     8581 tacaccaatt gctttcaaag gcgttagatc ttctaatgaa aaacccgctc gttccaatgc
     8641 ctctcctgaa atatcgacca tcaattctag tttattcttt ttcgcttcgc ttcctagttc
     8701 gatcaatcgc ttttgatatg ccgaaacatc atcttctggt atatgcatgg aagtgaaaat
     8761 accgacaaag ccattggatg ccatttggcg gatatactgt ttcttctctt ctgacaaaga
     8821 atcgttcata aatattgaaa agccaaacat attatccctc tttttctgta tccgtcttca
     8881 tttctatgac tagagtatat atctaaagaa acaaaatttc aatactttta ttaaaataat
     8941 taaatttcat ttctaaaggt tgagtttata ttatcaaaag aattgtcata acctaaaaaa
     9001 ggcactgcta agcaaaagca gtgccttgaa atattttatt taaataacac aaaaacataa
     9061 ttactcataa gaaaccaatt ctgtattatc taccatgaat gtaaagaacc cctcattagg
     9121 catcggatga aaataagcat tgatcactct tatcaaggca ttatggcaaa ctaacagata
     9181 gacattttct tcgtcttcga tgcattcttt caataaagga acgatccgtg catacacatc
     9241 taagactgac tccccatttg ggaatcgtac agcgaacgct aaacgtgctt tttgaaagtt
     9301 ctcatctttg cttggcagtc catcataatc cccaaaatcc atttctacca atcgttcatc
     9361 aacggtcagc ggcaagctga gacgatcagc aactgcttgt gctgtatctc ttgctcgttg
     9421 aagaggggaa tggatgattt tcgtgattgg aacaggcaaa tctactacct tttctgctag
     9481 tttctgtgcc tgcatctgtc ctttttcagt cagcggcaca tctgcccgtc cgcaaactaa
     9541 tccttgtgcg ttccaagttg tttctccgtg tctcgttaca taaagcatgt gattctctcc
     9601 ttcgttggca aagttcccct taaccatacc ttgttgttct tgagattaca agggttagat
     9661 tctctcaaga gaaataatct cagtagatac tttatccagg aaggtttgat catgttccac
     9721 tagtaacatg gcaggtttca cgttcaagat cagttgttct aattgttctt gattgaagac
     9781 atccaaataa ttcaatggtt catcccatgt atatagttca gctggctgtg ataaagattt
     9841 agccaattcc acttttttcc gttggcccat actcatctgc tcgatcttgt tatgaaaaac
     9901 atctctttcc atcccaagct ttcggaggtt gttcaaaaat gcttggtagt ctacttggtt
     9961 tttctctgca aattccgcca acgttcctcg attgtcttca taattttggc ttgcataact
    10021 aatgctcaga tgttttggct ggtatttttc tcctataacc ttgccgttga atgcccccag
    10081 aagataatgg ataatggatg actttcctgc accgttagga cctgaaatcg ccacccgctg
    10141 atgaggctcg attgtaaaat gaattggctc gaataacaga ttttcatacc ctaattgaag
    10201 atcttctacg cttaaaagtc gcttatggtg agacgcttgg ctattcatcg tcaaagaatc
    10261 gatatattcg atatctttta atagtttttc tttctcgctg atctgggtct ccatccgatt
    10321 cacgattgct ttggatcgtt tcatcgtccg tgcagcatca gcacccactg ctcctttatt
    10381 cactcgtcta gattcagtat cgatgaatcc gacttgtttc tttgttttat ctccttctcg
    10441 ggaacgagac cattcggctt tttcagctgc tgttttcttt agcctactga cttctttttt
    10501 caatttttca ttttgagcca tttcaaattc atcacgaagt tttttctgtt cttcatagat
    10561 agagaaattc ccttgataaa gttccagttg acttttttcg attgctaaaa catggtccac
    10621 tacttcatcg ataaatcccc ggtcatggct gaccacgatg aagccttgtt ttttcttttt
    10681 caaataagcc gctacttgtt ttctaccaga gatatccaaa tgattcgttg gttcatcgat
    10741 taacgggaaa tgagtgtcat ccacaaataa aagtgccagt aggacttttg tcttctctcc
    10801 ccccgatagt gtgctgaatt ctctccataa gatttcagga tctgtttgca tcaattggag
    10861 ctctctttcg atttcccata tctcaaaatc cgtaatatca tttaacacgt aataggttaa
    10921 acgttcttta tcttttgtct gttgcgggaa ataggcaaat tcttgctgat ggattacttg
    10981 cccttgataa ggtagtttgt tttgtagaat attcagtaaa gttgtctttc ctcgaccgtt
    11041 tcgtccgata agtcctagtt tccattgtgt gtcaaaattt agatttgctt gttcaaataa
    11101 taatgtgcct tggctgtcgt agccgaatgt cagatttttt atttcgattt tagacatttt
    11161 ccatcacctc gtaaaaaaag accagtccta ttttcagaac tggctaaaga ggatgcaagg
    11221 gttggataca cacaaaaaga cccatacatc gaaaaaacca agccaatccc gaaaatctgc
    11281 acactgcttc cttgtctcca caaggtacag tctgaatgca gctttcatca atcgacttaa
    11341 tttttcaatg cacggatccc tcactttgtc ctatatttga aattacgata gcataaagaa
    11401 aatgattttg tcaactactt cgggaggatg gtgaaaaatc tgctaagtgt ctgtcctaag
    11461 atttttgttt tttctaatgt ttgcctgtgt tccattgctt cttctaaacc tattggaccc
    11521 aaatgaatac ttaagacaat tgggagaaaa tcagaaagtc cttctagctc ttggtctcta
    11581 cttccgcaaa tagcgattat tggttttcga tatttttttg caagcactgc tacaccgtat
    11641 ggaacttttc cgtggatcgt ttgctgatcg atccgccctt ctcctgtaat gatgacatct
    11701 gcttgttcga tctttttttc caatccagtg atttggctga ctaaatcgaa accagaaacc
    11761 atcttcgctc ccaacaaaaa aagggctcca ccaataccgc ctgctgctcc acttcctgga
    11821 atatcatcta aatccacctc ccagaaatct tttatttgct gagcaatctt tcctgcttgt
    11881 ttatctaaga aagctagcgt cgtatctatt cctccttttt gtcttccaaa ttgccaagca
    11941 gctccttgtt gacctgtata tggattggta acatctgcag cgataatcaa ttttacttta
    12001 gaaacaagaa cgtttcttcg atcaaaatga agttttttag tagataataa aggatttcct
    12061 tctgtgtacc cttctaaaga agcacccaag gattggagta atcctagacc accatcgcta
    12121 cacccgcttc ctcctaaagt gacaataatt tgttctactt cttgaagcag tgcatcttta
    12181 ataagttctc ctaaggcata actggaagca agtcggatag tttgatccgt tggtttatca
    12241 agtagatcta accccaatgc ttgtgcagat tcgatgactg cggtgttttt tccttgccaa
    12301 gttgtcaata aatagctgat ttttcgctga cggaataaga gatccatccc atcaatggta
    12361 cgccatgttc ccctgagcgc gtaatagatt gcttctgcag tattttctcc gccatctgca
    12421 atcggcacat tgatttttct tcctttacga tcttcccagc catttagaac tgcctgattt
    12481 agttcaaatg aagaagcact tcctttaaat gaatcaattg ccgccaaaat atccatctgt
    12541 atccctcact ttcttcttaa gtaagacact ttttttcatt tttttcaaac aaaacaatgc
    12601 tattttcttc catttttcta tatactaaaa gggaactcat tgaataaagg agagaaaaaa
    12661 catgaaaatc gcaatagctg gggccggagc aatgggcagc cgaatcggat tgatgctaca
    12721 tcaaagcgga aatgaggtcc tcttgatcga tcgttggcct gctcacattg aagccattcg
    12781 tacaaatggg ttgattgctg actttaacgg taaagaagtg gtcgcaaaac tgccaatcta
    12841 ttcaccagaa gaaatcatcg aaagcaacga acacgtggac ttgatcgtag cattaacaaa
    12901 agccaatcaa ttagatgata tgttttgttc gatccaatcc attattactg acaatactta
    12961 tgttttgtgt ttactaaatg gattaggtca tgaagatgta ctggaaaaat atgtgccaaa
    13021 gaaaaatatc ttatttggga tcacgatgtg gacagctggt ctagctggtc ccggaaaagt
    13081 cacattgtta ggtgatggag aaatcgaact agagaacttg gaaccagaag gcgaagcatt
    13141 tacaaaaaaa gttgtagaag tctttcaaga agcaaattta aatccgatct atagccataa
    13201 cgttcgttat tccatttggc gaaaagcttg tgtaaatgga acattgaatg gtttatgcac
    13261 gattttggat tgtaatattg ctgaactagg cgcgcaaaaa gcggcagaga gcatggttcg
    13321 aacaatcgta tctgaatttg cttctatcgc agcaaaagaa ggaatcatac tagatcaaga
    13381 agaagtgtac caacatattg cttctactta tgatcctgac aacatcgggt tgcattaccc
    13441 atctatgtac caagacctga tcaaaaacca tcgtttgact gaaatcgact acatcaatgg
    13501 tgccatttgg cgaaaaggac aaaaatatga tattgctaca ccatattgtg cctttttgac
    13561 ccaattagtc catgcaaaag aaggaatcct tggcgcagaa taaaaggctc tctgatgaga
    13621 atcagctgaa tcagcagctg gttctctttt tttaagccca ataaaagatc aaaaagaaaa
    13681 cacaactctc atgatgccat aagaattgtg cttgtttgtt tcagtacata atctcttcaa
    13741 aatgtaggtt ttttatttgt tttcaacaga ggagaggtca tattcgtatt gctcataaat
    13801 agtggaatcg ttagtgataa atcgttttgc ccaattttct gcatcataaa attccaatag
    13861 ctctgatagg attggcttaa atggtgtatg cagaaattcc ggtaattcat ctaaatcacg
    13921 ttctcgaagc aaaaaaatat tttcaggacg ataaaaatga taatgagcaa tcgcttgatt
    13981 atccgtcact agtttctttt gaaaatacat ggattcaaat gtccgcaaag tcactccttg
    14041 ctgaccctcc cgcaacagtt ccagaatccc gcgcgattcc cctgtttttt tcaggtactc
    14101 ttcataagta agatatccag gttgttcatt ccctcgatgt ggaagaatga aatagttggt
    14161 cgtaacatct ctttttataa attcctgctt atagctttct gctcgttctt ttctcccatt
    14221 attcgttgca ccaaaaaata gatccgtctt tggttcgcta gaagaaaaat tcatatgttt
    14281 cgaataaaat gaactattat gtttcagccc aagttctttt gcttccaaaa aatcaaaatg
    14341 ataaatctca tcgattgccg gttcatcttg tgcctgcttc agcatcgcct cgtgttctgc
    14401 tttgaagtga ttccaaaaaa ataacacaat ttttgcattg gtaaatgggc gcacgcgtcg
    14461 agccatctcc atatcaaatc cataatctaa aaaaacgatc agctgataat ttgccaactg
    14521 ttgtctagct gatttaatga attccgactg tagtaaatct tttccggacc aaacatctaa
    14581 agagcggatg gaaggcaaaa aataaagtga tttatttttt gcaactagca gtacgtcatt
    14641 tttgttcatc ttttccgcct cactttctct gtcacttttt cctatgatag cctgtttaaa
    14701 tagtaaaaag ccagtaaaac gtttcctata tttgtatgaa aatttaacat ttgtatgaaa
    14761 atatgatgaa ataaatcatt cttctattaa aattcgccta aaagttttct gaatagttgt
    14821 aattgtctga aaatttttta tcattagtca ttgttaaata ttgctattta ttcttaatat
    14881 aaattttgat tgtgaattta aaagaaagag gtcatttatg gaagataaaa aattgaaaaa
    14941 acaacttagc tccagacata tcaccatgtt agctttaggt ggagcaatcg gtgcaggtct
    15001 atttaaagga agtggcgaag cgattgggat cgccggccct tctgtattga ttgctttttt
    15061 gattggtggg ctgattttat atatcgtgat gaaaggtctg ggaaaaatcg ttttaagcgg
    15121 tggcgacacg catcacggat tatctggttt gatacgccct tatctaggtg ctcattctgc
    15181 tgattttact gattgggtct actggtctat gtggattatc aatatcatcg ctgaagctgt
    15241 tgcagctgct tcctttttgc agttgtggtt cccgcacatt ccagcctggg tatttgtttt
    15301 tatgttggct gttttaacta cgatcatcaa tctttattcg gtacggttat tcgcagaaac
    15361 agaatattgg ctggcttttg ctaaaatcag tgtcattatc ttactgatca tttttgctac
    15421 ttatcttgtc ggtcagcaaa tgttgggcac aggcgttttc cctacccttc agcaattgac
    15481 tgaccatggt gggtttactc ctcatggtat gaagggaatc atcagttctt tgcttgtagt
    15541 tatctattct tacggcggtt ctgaactgat tgctatcacg gtaagcgaag cagatgatcc
    15601 taaacgtgcg attcctaaag caatcaaagg tgtcatggga agaatcattt ccttttacat
    15661 cattcctttg tttttgcttt tgattctttt tccttggaat actttagcag gcacgaatgt
    15721 cagtcctttt gtgatggtat ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa
    15781 ttttgtgatt gtccttgctt tgttctcatc tatcaattcg ggtgtgtatg catcttcaag
    15841 aattttgttc ttccgtttga aggatcgaaa aggttcctct agaaaactag ctgttttaaa
    15901 caagcatcaa gtaccgcaac gtgctgtttt attttgtacc agtgttcttt atcttggtgt
    15961 agctctttct tattttgtcg gtgacaaatt atttggctat ctggcaggtt ccctttcgta
    16021 tacagtatta ttgatctgga tcattatcag tgcggcatcc tttgtactgg cactaagaaa
    16081 aggaacatta tgggataaag gaatcagctt ttttgcatta gctgtcttag gattgatttt
    16141 ccttggtatc ctgttcacga actccattgg ggtaactgta ttgactggcc tgctttacct
    16201 gttcatttat ttcagttatc aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa
    16261 tcgatagaaa attttatttt atttatgtgg aatagatgga gctattgaag aaaggagttg
    16321 tgacaaaagt cttgttgcaa ctcctttttc cgaataaaca gtctcatgac ctcttggtaa
    16381 acggtgttca aaaaccctct gtgcaaaata attgattgtt ctccatgctc atgacatgat
    16441 agaagaaagg aatgaaaaaa aggagtttgt tttaaatgaa aaagtattgg aagtttttgt
    16501 tgatgatcgg tgtttcaacg gtagttatgt ttggtatgat gtacttgaat gtctatgcgc
    16561 tcgatcatat cttcttcagt cgtacacgag tattcatggc gttgatgatg ggtgcagtca
    16621 tggcagtcat catgctgttg ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta
    16681 tgatcttagc cgctagtctt cttgtttttg ccggttcctt gtttatggta cgaagccaaa
    16741 ctactgtcaa cgacacagct tggatgaagg ccatgattcc tcatcattct atcgctatcc
    16801 tggcaagtaa acgagctgat ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca
    16861 ttcaggcaca agaagaagag atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac
    16921 cattatccgt ccaaataaaa aaagaggatg ttccagaagt tatcagcttc aagaaataag
    16981 aagaagctac ttctggaacg ccgtgaataa ggggttgtga aaaagctgcc ctgcatcaag
    17041 taataagaac agccaaacaa aaatagcttc tcctattttt cgtttggtta ggcttattac
    17101 cgcagatgca agcttttgaa caccgtttat taagaggttg taaaatcagc gttctgacct
    17161 gagtattaga gagacaaatg gaaaaatagt tcctcatatt ttgccatttt ttgagctaat
    17221 accgagggtc agctgattga acaccgttta tcgagaggtt gtgaaaaagt cgcttagttt
    17281 cgcgtattaa gaaaaatttt gtgaaatggc tttccacatt tttgtataaa tttggcttaa
    17341 taccgagaaa ctggcttttg aataccgttt attcgtaaaa gggattgtga caaaagtctt
    17401 gtcacaatcc ctttttatgt ttttattgtt ctaattgcgc tgtcttgctt actgcttctt
    17461 gcggtttcac aaattttttt gagattcccc aacctgtgaa ggcactgacc atcgaaaaca
    17521 caggagaaag caaactgaaa aatacgaatg gcaagtaagt gagtgtagga ataccaaatg
    17581 tacttgctac gaaacttccg gcaattcccc aaggcactaa gtagttgatt actgttccac
    17641 catcttctag cgttctacct aatacagttg gatcaaatcc tgctttttca taagtttctt
    17701 taaatgcatt accaggcaga ataacagata ggaattgttc accaataaaa atatttactc
    17761 cgatacttgt catcaatgtt gtaaaaatca aatttggtgt gttcgtcatc tttcttgata
    17821 ctcgttccat cacggttgag atcaagccaa attcgatcaa caatccacct aatgacaaag
    17881 ttagaataat caatgacaca gtaggcatca tactttcgat tcctccgcgg ctaagcagtg
    17941 aatcgatttg ttggtttcca gtatttgaaa caaaccctga ttcgatgacc ccagccaact
    18001 tcgttaagga agtttgtggg ttttgaataa aaatcattcc tactgctaca gcgatattca
    18061 acaaaatcgt taaaattgcc ggcacctttt tccatgcaca cagtaacatt aaaaataaag
    18121 gaatcagtga ccagattgaa atagtaaagt ttgcttctag aacatcagtc acttcacgaa
    18181 tagatgaaag acttgcggct ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa
    18241 caacaaatgc cgggacagtt gaccacatca tatgcttgat gtgtttgaac aaatcggcat
    18301 caacaatagc tgctgctaga tttgttgatt cggataatgg agacatctta tctccaaaaa
    18361 cggcacctga aacaatagct ccaacaacca gcgctggatt taagcctagt gttgtcccaa
    18421 taccgaaaaa tgcaatccca atcgttgaca taacagtaaa ggcactccct acagcgctac
    18481 ctacgattgc acacacaaca aatacagaag ggacaaacca ctggacactg atcaatttga
    18541 acccaatgac cattagagtt ggaatgatcc ctgcttgtat ccatacagca atcaatgaac
    18601 cgactaaaat aaaaataaag attgggatga ttcctggttt gatgccattg atgattcctt
    18661 cgtgcagcca atctgtcggg tatcttttta tcactgcata caacatgacc attccgatcg
    18721 ctgctaagat cgtgatatta ggtgagacct ttaatccgac aacgccaatc gcaatgccta
    18781 aaacaataac cattaataca agtgttgctt ctaaaaaact tggtgttcct ttttttccca
    18841 tgataatctc tcctttttta gttgttctta tgttctaaaa aagttaagat atcggattac
    18901 tccgccgcaa ttcatccgcg attcctcctt tataaaaata aaaagacctg ttttaaacta
    18961 aggacgaatg actccgcggt accaccttaa ttaaaaacag atctgttttt cactttcaaa
    19021 cttttattca aatgtatgga ctttccatgt aattcgtcaa ttaggcttga tcggttcaca
    19081 gcgaccaccg actttctgaa caaaacacct aaccgctact gcgtgaaaag ttgacttgta
    19141 cgaagtttac cttccataag atcatttgtc aatgaaaaag aaaatatttt ttcatttttc
    19201 tgaaaattat ttttataact ttcacacaca agattatttt ttactagaaa gtagcgccct
    19261 cttgtttttt tcttgctact atttcttgat gttcttgctt gattctttct aatagctttg
    19321 gctctttcaa cagatcaaga gctgtattgg cgagtagttc tgctgcaatc gcaatagaag
    19381 caaggccttt gtcactttta gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg
    19441 tctcagagat tgctaccgta ggctggattg ttggtatgac ttggctgaca tttcctacat
    19501 cagaagaacc gagacttgtt ctttgttccg tttctatttc ttcatctgga acacctgctt
    19561 cttttgcatg ctggaagaat aagtcatcaa aagaaggagt aacgatgaca tcatcaactg
    19621 cattttggaa caatccaaac tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga
    19681 tattttccac tttttcatat actttatcta atgtttgccg atttcctgca cgtaaataga
    19741 aacgtccagc agcgtaatct ggaatgacgt tggcagctac tcctccgtct gtaatgatcc
    19801 catggatatt gacatctttt ggcaattgca agcgtagagc gttgattcca ttaaacactt
    19861 ggatcaatgc ttccaaagca tttactcctt tttcaggtgc tgctgctgca tgggaagcga
    19921 caccgtaaaa tttgatgtcg actggatcat tcgctaaaga ttctgtcgtt ttcccataac
    19981 gaaaagctgg atggacgcat aacgctacat cgacatcctc aaaaaaacct tctcgaacaa
    20041 agctgccttt agctgatccg ttctcgcccc cttcttcccc tggggtgcca tatacacgga
    20101 tttctccacc agtttcatca atgaattgct tcactatgct ggcggctaat acggagtaag
    20161 taccaaacaa attatgcccg catgcatgac caatacctgg taaagcatcg aattctgcca
    20221 aaaaggcaat tgtgggaccg ggtttattgg aaatataacg tgcatcaaag gctgttcggt
    20281 gtccagctac ttctttttct acccaaaacc tttctttttc taattgttta atcaacgctg
    20341 ctgaggaata aaactcatag ttacttactt caggatgttc gtggatatct agagcaagtg
    20401 cttgatattc tggtaaacga tattggatta gatccttcaa ttggtttgtt gagattgttg
    20461 ttgtcataaa aaattcctcc tatgctagtc atttttctta ttttctcccg aatgtttccc
    20521 atgcaggcac gcttgatcct tttgatgttt tctcgattac tttgactgtg tcttctgttt
    20581 gataagcgtc tactattttt tgatacgttt tattgttttc atcttctttt cttgcaacga
    20641 tgatattcac ataaggacgt gcatggtcat ccactggttc taagaagatg gcatcagaag
    20701 caggattaaa tccagcatct acagctacac cgctattgat cactgaaatg tctacatctg
    20761 acaaagcacg tgctgtttgg gaagcatcta attcagaaat gtctagattt tttctattct
    20821 cggtaatatc agaaacagtg ggcgcttgtc cttttgcctt atccactttg atcaatccag
    20881 cagtttgcaa taacagaaga gctctgccgc catttgtaac atcattaggg attgctactt
    20941 ttgctccgtc tttgatatca gctacatcct tcaaagtgtt agaataaatt cccagcggtg
    21001 cattgacagt atttccgatc gatactaaat cagttccgtg ttctttattg taattgtcta
    21061 aaaagaattg atgttggaaa gaattcaaat caatcgatcc atctgctaaa gccacatttg
    21121 gttgattgta atctgtaaat ttcacgtatt ccaaattaat gttctccttt ttcaaacggt
    21181 ctttcacgtt gtcccataca tctgtatctt ctccaataat ccctagtttt acgatagttt
    21241 cttttttgtc agttgtatct gcttttcctt ggctacaccc tgccaaccaa ataattgcta
    21301 caaatgtaat gactacaaaa cgaataattt ttttcatttt ttctcctcca ctttcaatgt
    21361 gtaatttttt taatcagttg attactgata aattgactta aaaaaactaa gattagaatc
    21421 aataatgtag ccacaatcgt tacatctgtt tggaatcgat tataccctct cgatatagca
    21481 agattcccta atccgcctgc acctactgct ccagccatgg tagtcagtcc gatcacgttg
    21541 atgatagtca acgccgagac ccgaatgata ccaggcaaac cttctaacaa atacacacgt
    21601 aaaatgatac cgattggact ggttcccatc gcttctgccg cttcgatcac cccaggatca
    21661 acttctagca gtgcgttctc gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat
    21721 ggcactaaag ctgctttcgc accaatcgtc gttccaacta agaaacgagt agtcaatgct
    21781 aaaagagaaa gcaagataat gaaagggatg gaacgaatga tattgatcag cttatcaagt
    21841 atttcaaata gcagacggtt agctagtaat ccattagggc gtgtcactac taacgtcaca
    21901 cctaagatga ttccaatcat cccgccaatg aatgctgtcc aaaaagacat atatagtgtt
    21961 tgcttcgtcg cttcgacaaa ttcatccgga atcaaataaa cattgggcaa atatgtttta
    22021 aagaaatcag tcattttatc ttcctccttc gatcactttg atacgactat ctaaatcgtt
    22081 tttttgtaaa atatttattt ttacaccaga tttgatcaaa taatccaatg cttttttccg
    22141 ctgtttggtt tctccagcta aagtaacgat caaactccct aacggcgttt cttgtaataa
    22201 ctctacattg ccatataaaa tattcgctgt tacttgatat ttgctgtaca gatgagcgat
    22261 tagaggttcg ttcgtctgat ttccaatata acttaattcc accagccatt tattggcgat
    22321 ctgatcggca aaagcagaat gggcaatgat cgtttctaat gcttggtcaa gatgagtagc
    22381 tgttcgaata aaatctttgg tcaattcttc ttcaggattg ctgaagatct ggatgctgct
    22441 acctttttca acgattcttc catcttccat aaccgctacc ttgttgcaga tttctttgac
    22501 aacttgcatc tcatgagtga tcaggacgat tgttaaacct aactggcgat tcaatttttt
    22561 taacaatgca aggatctgaa gggtcgtttt cggatcaagt gcactggttg cttcatcaca
    22621 taataatacc ttaggttcat tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc
    22681 ccccgacagt tgtttaggat aagcagatgc tttttcttcc aaaccgacaa gttccagtag
    22741 ttcattcact ttttgacgac gttcctgttt cgattttcca gaatacttta aagaaaaatc
    22801 aacgttgtca aaaatagttc ggctttccat tagattgaaa tgctgaaaga tcataccgat
    22861 ggcctttctt tcttttcgca attctttggg agagagctga ctcagattcg tctgattgac
    22921 aatgacttct cctgctgttg gtttctgcag caaattcatc acacgaacta acgtactttt
    22981 ccctgcacct gagtaaccga cgattccata aacatcgcct ttttcaatcg ttaaatctac
    23041 agaatctact gcctgtatct ctttttcttt ttgtttgaaa catacagaaa catctcgcaa
    23101 ttcaatcatt aaaattcctc cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca
    23161 ttttcttgag gacgcttttt tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca
    23221 actcctcttc aagtacgatc atactctagc aagatatcgg tcgcagccgt catcgcctta
    23281 catctatgct cgggatgaat tgctcgatgg ccattttcca atagtcgttc cttgcttctt
    23341 ctcagctacc gaagctctct gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa
    23401 taatattcaa aaaaaaagtc cttttactaa cataaactat gtcagtaaaa ggacgacgta
    23461 tcgcggtacc accttttttc ggaagtttcc ttccctcaag cccaacatct caaaaagaga
    23521 gattggcgtt cgttttaacg gtcgaacagt tccgaatttg cctacttatc gacaaattat
    23581 tctccaaggc cattttcagc ttttcttact tgactcttct caccagccag agcctctctg
    23641 caaagtttcc aaacttactt tccttttcaa cgaaatcgat attttgttgt tgggattaat
    23701 ctacatgagg aaaactgaaa agtcaagtac gaaattcaaa ttattttttc tatatttatt
    23761 tttctgtttt tttatttaac atttcaataa cagttcgaag atcttttcct tccgcaagaa
    23821 gtttcaattt ttccttttct tcgacggtat aatttcggac aaaactggaa tcaggtatca
    23881 aaacaacgcc taatcctgct cgtgaagcag ctgttgctcc tactaaagaa tcttccactg
    23941 caaaggctga actagcttct aatcctgttc gttccaaagc tgtcagataa ggaatcggtg
    24001 aaggcttgtt ctccgacact tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt
    24061 taaagtgatc caagatatct gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt
    24121 tcatatgacg ttcttttaag tctgccaata tttcttttgc atatggtttt aagtggatgg
    24181 ctccgcttgt cagctgttga taaaaatatt cttcacgctt cgctcggact tcctgtactt
    24241 tttcagggct acctgctgct ttcactaaaa tatcaaaact ttgtttccag ttttttccgc
    24301 cccaagcgtc aacgatttct tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc
    24361 agccatcacg atacgttttt tctgtgtcaa ctaacaaacc atctaaatca aatattgcac
    24421 ctttcatcta tacattaatc ctttccaaaa caatacacat agtttagcac gactggtcca
    24481 aaaagaacag aagcaaagaa ccagccttct cgtttataag agttcttgga atcgacggat
    24541 atagattttc ttcacagctg tcacaagcgc cagataagcg atgactgtag caaataacca
    24601 taaccagtaa ttgccaggaa gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg
    24661 aaggacagaa ccgacaataa ttcctaatgt agtgataaca aacatggcaa atgttgcgtt
    24721 gctttggata aatggtactt tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca
    24781 tagtgattcg acaaaccatc ctgcatggaa taatgcgatg aaaatcactc gctgtgaact
    24841 atccaatgta tggaaacttc cgcccactac agcaggacaa ataacaaaat acatcaacaa
    24901 gtaagtagta atatcaaata tcgaacttgt tggaccaaag taaaccatga attccccaat
    24961 actagatgca ttccattttt ttggctcgtc caaatattct ttatccatcc gatcccatgg
    25021 caaggaaatg caggaaacat catagatcag attcaaaaac aaaagttgta aaggcagcat
    25081 tggtaaaaat ggcaggaacg tacttgcaat cagcacggaa aacatattgc caaaattaga
    25141 acttgctgta gccttgatat atttcatgat attcccaaaa gtttctcgtc cagaaagaag
    25201 gcctctttcc aaaatcgtca aatctttttc taaaaggatc acgtctgcag attctttcgc
    25261 aatatccaca gctgtatcta cagaaatacc gatatcagcc actttcattg caggagcgtc
    25321 attgatacca tctcctaaaa atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa
    25381 acgtgctttt tgctgaggat tcaattttac gaatacttca tggtcttcag ctacttgtgc
    25441 taattcatga tcactcattt ccgagatttt ctcaccggta accaattcat tgatctctag
    25501 acctacttga cggcacactg aacgtgtaac taattcgttg tccccagtca gaacttttac
    25561 agctacacca tgttctttca gagctttcaa cgcttgtttc gttgtttctt ttggtggatc
    25621 aaggaaagca agataaccaa tcaaaaccat gtctgactca tctttgactg aaaattctcc
    25681 aacgacgctt ggattggtct tttgtgcgac accaatcact cgtaagccat cttcattaag
    25741 tgactgtact tttgcaagta cggcttcttt tcgttctttt gttaatggtg taacagtacc
    25801 gccaagatca ataaatttag aaatgttcag catttcttca atcgcacctt ttgtgatcat
    25861 ttgtgtttta ccagctggat cagcaaccac aacactcata cgacgacgtt caaaatcaaa
    25921 aggaatttca tccacttttt gataattgat cttatctgta tctaaagttt ctgttgcttc
    25981 atcaatgata gccacatcta acaaattttt cagccctgtt tgatagtagc tatttagata
    26041 cgcatggcgc aaaacacgat catcttcatt tccttcacaa tccatatgat attcaagaat
    26101 gattttatct tgtgtcaatg tccccgtttt atccgtacat aacacatcga ttgcaccaaa
    26161 gttctggatg gcattcaaat ttttgatcac tgttcctttt ttggccatca cacttgctcc
    26221 tttgaccaaa ttcgtcgtaa cgatcattgg aagcatttca ggtgttagac caactgctac
    26281 agatagtcca aaaagaaaag cttctagcca atcccctttt gtaagcccat tgatgacgat
    26341 aactgctgga gccatcaatg acataaattt gatcagtagc atggatgttt tatggatgcc
    26401 taattcaaag cttgattgaa ttggtttttc agatagcgtt tctgctacat gaccaaatag
    26461 tgtttcattt ccaacagaaa caatgattcc ttccgcactt ccactgatga cattacttcc
    26521 cataaagaca agattttcgt aacttgtttc gctttgctga tcgtcttcac atactttcgc
    26581 atgcttttct actggatagc tttcccctgt caatgctgct tgggaaatga ataaatcctt
    26641 tgcggacaat aaacgcatat ccgctggtat catatcccct gctgaaagcc gaacaagatc
    26701 tccacagacg atttcttcca taggaatctc tgtgtacttt ccattacgtt ttacagctgc
    26761 cgttactttc acaagtgatt tcagtttttc agccgcttga ttggaacgta cggactgaat
    26821 caacgtcatt gtcccgctga cgataaccat agtgaagata atggctacac cgaaaagatc
    26881 tttatctcca ggagctgcta atacatattc cgtgatgaat gaaataattc ctaatgcgat
    26941 caacactaat gtaaatggcg tgatgtatgc tttcaatatt tctacaatga atggtgtttt
    27001 tttgccatat gaaatcgcat tttcaccgta attttctctt gaacgttcga tttgttcttc
    27061 tgtcaatcca tttgctgatg catggaatag ttcaaataat tcttgtttcg tttttccagc
    27121 aaaagctgca tagatttttt gtgtatcttt ctttttttcc atctcaatct ctcctttgtg
    27181 tccaaaagac agctgttctc gctgcttttg gtctttagct attcatcgga tcgatagaga
    27241 aaaaactgaa aggggctgag aacagataga taatagccct ggcctttcct ctagatcctt
    27301 gtttttcgaa tgactgtcat ccatttgttc tcatctcctt tactaagtaa ttttaattca
    27361 tgacttccaa tcatttttcg aatagttatt tttccaatta gaccacctcc tttttatttt
    27421 tcaccgatgg ggatttgcgg caacaaaaaa acctctcgcc aaatactcag cgaaaggttt
    27481 gaaaaagcat attaaaaaaa gcttctcaga tctcatcgtt gagttttgac actgtacaac
    27541 gtaaaaagca gatacctgct tttagctacc ttatgaaaag ccttatctcg acaattcctg
    27601 ttctacccat tggcgtctct cgacgttttt gggcagtagc ctatgtttgt ataggagcct
    27661 cacctaacaa atcttattcg gttttccttc gttaatataa caactaaaaa agaggatgtc
    27721 aaatcaattt tccatgtttt tcatactttt tttaaaatac acacgttata ttcctgtctc
    27781 acgcggtttt atttgtctca ttttccacct cccgctataa tagaaatata gtaggaaacg
    27841 aggtgctgtt atggaaagaa cacttgttat tatcaagcca gacggcgtga gacgccattt
    27901 agtcggaagc atcatccaac gttttgaagc aaagggactg gccattgctg aaatgaagtt
    27961 tgaaactatg actcctgaat tagcaaaaga acattatcag cacttgactg agcgcagttt
    28021 ttttgatgaa ctgatcgatt acatgacttc cggtcctgtc gtttatcttg tgctggtcgg
    28081 cgaggaagtg atcgatatcg tccgtaagat ggttggagca acaaaagctg ccgatgctgt
    28141 tccaggtacg atccgaggag actacgcgct gccggggact gaaaacatca ttcacgcttc
    28201 tgattcccgt gatgctgcgg taaaagagat cgcccgcttt ttcccgccat tcgatataaa
    28261 caaaccgtct gaaaaagcgg cgaagtgata caaattctaa actgctggtc tagttgctgg
    28321 gttcagcaat ggccagcagt tttttctcta aggacctccg ttgattcttt cctaacatat
    28381 attcgagcac tgtgaagaat tcttggtagt ctttcgtctt ttcttcccct aaagcagcag
    28441 aaatcaatct ttgataataa tctgccatca atgtttcata gatcactgta cttctttctt
    28501 tatgtgctaa gctccgtttc atatcttcat tcacactacg tgcctttttc aaccgaccat
    28561 tttctatata aaacgaagtc aggttcatta aaaaagtaaa gagatactgc tcatgattgt
    28621 tcaactgtgc taatcgtcgc attctttttt tgattttcaa ataattgttc tcaatgaacc
    28681 gatcactgaa tatccaagga aaatcagcta gaatagatag ttcaaattga ccatagtctg
    28741 gtatctgcat caagtattct tcaatgattc ggcattcata acgtaaaaat tcaaattctg
    28801 tctcagatac ttctgttttt tcgaccatgt gaagattcaa aaccaaataa tacagatctt
    28861 ctgttttctt aaaatgccta cggttattgc tgatctcttt ataaggatca aagctgtctt
    28921 cttgtacccg atctagcatt cgtctttttt tctttaactt tattggttca tccagctggt
    28981 ttcgcatcca ttcatattct gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg
    29041 ttacaaatga catttttttc actgaatttg tctcaatccc gactagagaa gtcttaaaac
    29101 agattccttc cgctaattgg gactgtgtca acttcttgtt gactcgaagt ttcttgatca
    29161 attcaccatc atacataata gttctccttt ttaatttaaa tttttccaca ttcaataaat
    29221 agatacgtta aaaaggaaaa atacacgaaa aaaataaaaa aatgtttttt tcataaaaaa
    29281 taccaacctt caagcattag aatgttgtct aactactgaa ggttggtttg attgtttatt
    29341 gattattttt ttgctgttca acgtctgttc ttgttactgg atataagttg gttgttgttt
    29401 tattaccatt ttttgaaaag acacttgtcg aatgttcgcc tttttgcttt tcgattgctt
    29461 cattacgttc cgcttcattt ttatagtcat attttttcgg atcaatcgtc tcaaatccgt
    29521 ttggcacata aaaccgcaag agattttcct gattcagctt atctgaaaga cttaacgcag
    29581 tcttgacttt ttcctgatct ttggagattt cctcatcgat tcctgctgct ttcgtatcga
    29641 tgatttctcc tgtctgatta ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca
    29701 caaagttccc atttcgaaaa gcaactactt gatcatgctg agaagataat aagtctgtac
    29761 caaaatgaag ataatcttta tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat
    29821 cgatttctcc accgtattca tgattgactg tccctttagt ataaccaggc atatggatca
    29881 tcaatggcac tcgttgcatc tgtgcgttgt caaattcatc ccatgtatct ggatctttac
    29941 caagagcgct tgctaaatct ttattatctg tattcgagat cccaaaatga tccccgtaaa
    30001 tcacaaagat tgaattttgg taaataccag attttttcaa atacgtaaaa aattgttcca
    30061 atgattgatc cagataatgt gccgttcgaa cataattatc tactgtgcta ttgccagtat
    30121 tcaaagatgg aaagtcgaaa ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg
    30181 aaagaaattt cgtgtaaaac ggctgttgta gatgctcaag atattgggca gattctctga
    30241 ataaatcttt atctaatatc ccataaccta acgtttcgtc ggattcatca aaataagatc
    30301 gatcaaagaa attgtcataa cctaagtttt tatatacatg atctctattc caaaaactag
    30361 ctacattccc atggaaaaca gcgcttgtat agccttgctt ttgtccaagg atcgctggtg
    30421 cggcttgaaa gacattgtct gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa
    30481 atgtgccagt ttccaacata ttttcagcat cacttgtctt tccctgtcct acttcatgaa
    30541 agaaattgtc aaagctgatc gttgcttgat tctggaaaat actatttaaa aacggtgtga
    30601 cttcttgtcc gtctaccttc atattaatca aaaactgctg gaaactttct aaatggagaa
    30661 cgatcacatt tttcccttta gcgatcccaa atgtttcggg atttggctct gcataatgtt
    30721 tctttgtata atctaaaact tcatcaatcc cattactgct tgcatgcgcg cgaacactgc
    30781 tagtcattcc tgttttgatt ccgtcataaa tagtatacgc atcgattcct agatatttga
    30841 caatatatga acggtcaaat gttcgctgca gcaattgtgg acggttggct tcgctcagcg
    30901 ctagatttcc tgaaaaaata agccctgaaa gacaggtaac agcgatagcc atcggtttgc
    30961 cgacagggtt gcttttgata ggtattttct tgaccttcaa ccaaaccagt agcccaatga
    31021 acacagcgat atccagccag tagatgacat catggggctt catcaaagaa aaggagcttc
    31081 ctgacaatcc ttgcgacact ttcgaaaagc ctaatacaga tttgactgta atgaaatctg
    31141 tgaattcccg ataaaaaata atattcaagt acaacaaaag cgtatttaaa atatcaatga
    31201 tcaaaagaat agctaacgct ggctttggct ttttaatata caaagaaagg ccaaaaaaca
    31261 ataaggtagt cgcaattggg ttgatccaca agataaagta ctggatcgta ccttccacac
    31321 ctaatgaaaa atctgcatag taggcaatga tcgtcttcag ccagaaaaag aaaacagctg
    31381 tcaaaacgat tgctaaacga ttttggcaca attttttaag ttgtgtcacg tatatcactt
    31441 ctctctattc acattttttc ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt
    31501 gttatcttaa gattttttta ttctttctaa agattattcc gtccactttt cacagcataa
    31561 aaccgctgcg atgtaagcga ttaaactaat gaagagataa aagtcttgtg gtgaccgctt
    31621 cactccacgg tattcaaaag ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca
    31681 aaattcttgt cgcagctcct tatttcttga tgcaggacag ctttttcaca acttcttcct
    31741 taaacggtgt tctcctgcca actcctcggc cacaagtcac aatatttata aaacatgaga
    31801 agctgttttt tgaaattgtt ccttgtggct cagagttaaa cggcaggctc aagacctctt
    31861 gatacacggt gttcaatcag ctgaccctcg gtattagatt atgtcaggac gctaatttta
    31921 caacctcttg gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg
    31981 aaaaatataa ggagctattt tttgaaattg ctccttgtgg ctcagagtta aacggcaggc
    32041 tcaagacctc ttgatacacg gtgttcaatc agctgacctt cggtattagc tcaaaaaacg
    32101 gcaaaatatg aggaactatt ttgccgtttg tttctctaat actcaggtca aaacgctgat
    32161 ttcacaacct ctgtttttca ttgtccatag gattgatatt gtccttgtga tgactcattc
    32221 gtaataattg aggcttgatt ttcttcactt cccgttatcg tttgtccact gccagaatct
    32281 gaaggacttg ttcctgtcga ctcgtttttt tgagaagaga cagttgtttt cttgacttgt
    32341 tcatttgtct ttttccattc cgctaaacga tcggcgacca cttttttgtc tactttttca
    32401 gaataagcgg tgatcacttt tccatttttc aaacgggcta ctttgtcgcc atcatgagtc
    32461 aaaagcaaaa cggtcaaatg cttgtcttta aattgatatt gtctgagaga aatcgcacta
    32521 agtacgtcat cgatattaat aaattgattt tcttcttttc cttttggcaa ataaatgtcc
    32581 aattcgcctt tttcgtaatt ggcagttatt ttatactccg cccaccaata agttgagaat
    32641 tcttttttga aattatctac tgattctttg cttgctacga ttttttctgt ttctttctga
    32701 gcaagatctg ctgtcatttt tcctgaaaat gctgcggata aaataagcag taagaaaaaa
    32761 ccgaataacc aaaagagaag aaacggtctt tttttcctca tgctttcctc cactcctatt
    32821 taattttgac tattctatta tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa
    32881 gatcgtaaat aattgaactt ctttttcaca aaatggtaag aatggagaat ttcacgcatt
    32941 tgctctcaac tattctttct taagcctcaa atccgttttt atctgatacg tctttgaacc
    33001 aatgacccga ttttttgatt gttctttttc catcttgtgc taagtctact gaaataaagc
    33061 catatcgatt cttatatgca ttggtccacg accagttatc catacatgtc cacatatgat
    33121 agcctaaaca attggcacct tcttgaatgg cttgatggac atatttcaga tgagattgga
    33181 caaattcgat ccgataatca tcgtggatca tgccatcttc tttgataaat cgtgcttcgt
    33241 tttctacacc cataccgttt tcagaaataa agcagtcgat atttccataa tgatctcgta
    33301 cattgatcaa aatatcatag attccttgtt catagatctc ccatccgcga taaggattca
    33361 ttttacgacc aggcatttca taattgtcga aataatcctc aggcattgga ccgtttttag
    33421 tatcgattgg tgtttctttt gcttttaccc ggcgtggctg gtaataattg atacctaaaa
    33481 gatctactgt attttctttg ataaccgtca aatcctctgc atggatcaca ggcatgagat
    33541 caagttcttt tactatctca atcaattcac ttggaaactc gccttttaca gctggatcaa
    33601 ggaatgaacg attgaagaac gcatctgcta tttgcgctgc tttaacatct tcagggtttg
    33661 attcgtctct tggatagctt ggtgtcagat tcaagatgat accaataaca ccaccttgct
    33721 ccatttcatg gtaagatttg atggctaacg ctgaagccaa cgcttcatga tagccaactt
    33781 ggatcgcttt tttcatatct gactcttctg gataatgcca tcgatataaa taacctgctt
    33841 cgactggaac gattggttca ttatgagtaa accatttttt tactttcgaa ccaaacaatt
    33901 caaaacaact tttggcatat ttttcatagg cttcaaccgt ttctcggttc aaccatccgc
    33961 ctttttcttg cagatacatc ggcatatcga aatgatacag attcacaaag ggttcgatac
    34021 cgttttcaat caactcatca aaataacgat gatagaaacg aacggcttct tcgttgattg
    34081 ttccagattc atcaggtatc aaacggctcc attggataga tgttcgaaat gaattatgcc
    34141 cagtctgttt catcaactgt acatcctctt gatacttcgt ataaacttgc gaggtttttt
    34201 caggtcctac ttgatcaaag aacttttgcg gttctttttt gtaccaataa tcaaaaatgt
    34261 tctctccttt tccatcgcct gaaatccttc cttctgtttg tgggccactt gctgcagaac
    34321 cccaccaaaa atttgctgga aattgatatt ttttcatcaa agcacctctt tttctttttt
    34381 agttcgtttt agttgattcc atgtatgata ttttccatca caatcacttt ttcattttct
    34441 tcctcttctt ttttgattcg ttttaacagc tgttctgctg ctcgttcccc gattttatct
    34501 gttggtggcg tgatcgtgat tttcggaagg atactcagat cgtttttatc taaataatca
    34561 tagtgactga acgcaatatc cttcccgatt ctgacacctt cttgattgaa acattcgatg
    34621 gcaccaatcg acatattgta attgctgaca aaaatagcag ttgccccttt ttctaataat
    34681 gcttttgccc cgtaatagcc gctttttcta gaatagtctc ctgtaaagac ctctaattgg
    34741 atcgatggat cattttggac cgcttctttg gctcctgcta aacgttctct tgctacgtag
    34801 tctgtttgag tggcagcaat aaatccgatt tttttatgtc cttgttcacc atgatgagcg
    34861 atcacttttg ctacactttc tcgatcattc actaatattg tatcaatacc tgctggtcct
    34921 tctggacaat tcaatgcgat aactggaata ctcatttttg tgagcatttc aaatccagac
    34981 cactcttctt cggataagaa gacaatcagc ccatcgatca catgcttcat caggtattca
    35041 attttttgtt caattttttc tggctgatcg gcaaacccgc ttaataataa gctataccca
    35101 ttttcttcag ctactctttc gatccctgag acaacttccg acccaaaacg gctggaaatc
    35161 ccgtttatca aaagtccaat agagaagctt cgattattct tcaacccttt agcaatgatg
    35221 ttttctttat agtccaacgc tgaaatcgct tctgctattt ttttcatgtt cttttccttc
    35281 agttgctgac cattcaagta acgggaaact gttcccactg aaacaccagc catcttagcc
    35341 acatcacgga tcgatgccat acattctcac tcctgccagt tacatttgat ttctctttct
    35401 tgttgttcga ttatgatgac cgcattcttt gcatagtgtt gcttgtcatc aatatccata
    35461 taactaaagt cttcagtaga gcaacttcct ctcactatta attatactta aacaagtatt
    35521 tgaaccggtt catttttaat atagcatttt tttaaaagat tgcaacactt ttttaaaacg
    35581 ctttctgaaa gtggcggatt ttttgccttt ttaaaatttt attctaaaat ttactctatt
    35641 tgtgggtgtt ttgtgcgttt ttctcttcca tccgtttgaa gttttacatt ccaatggtat
    35701 aatttcttta tcctatcaaa tgggggaagt attttgaaga aattaatcgt actatttatt
    35761 ttgtctgcgg gacttttgac attgactggc tgtgatttgt ttggcggctc tgaccaatcc
    35821 tcggacgaaa caaccgattc ttctgtctta aaagcggaaa aagcagatac caccaacagt
    35881 gtatatacgc taaaagtacg taaagatatg gatttatccg cgggacagtt gacatttaca
    35941 gataaaaaaa tggaatggac acgcacgtat cagacacaag gaactgattc ttctgaatca
    36001 tctgaagaaa aaacagtgct gacagatatc aagatcacta ccaaagatga cgaatatatt
    36061 atttcaggaa aagaaaataa aaaagaaaca acagtcatat ttacaaaaat cggtaattac
    36121 cgtttgaagg atgaggatgg caacatctac agtctatagt gccgtctgtt ggatcttctt
    36181 ttgtttctcc ccttcttttc tgctatagta aagaagcaag atgattgtaa aggagacgaa
    36241 atggatgatc gatagtcctg attattgtac atgtaataac aacaaaaata tccatacaga
    36301 atttaatgac caaggaagct tgagcatatg cgacaactgt cacaaactag tcaaagattc
    36361 atttaaaaca tttgaatctt atcctgttca aaattatgta aaaagtagaa gaagataaac
    36421 aaatccaagc aaaactcact atggctgatt tttatgaact tcaaactcag cttcttcctt
    36481 tgatcaagct tcatcagtaa aacaaacagt ggatcatttc agtttacaaa tcaaagagta
    36541 aagatttatt tatttcaaat atttgaatat aaaataacca aacgatagaa aggcttattc
    36601 tacatcacgt ag
//
LOCUS       DAYYAR010000013        36217 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000013 DAYYAR010000000
VERSION     DAYYAR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.013"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1500
                     /locus_tag="ACHAT0_000547"
     CDS             <1..1500
                     /locus_tag="ACHAT0_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFM8450516.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDD
                     TLLEVQNDKSVEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="ACHAT0_000548"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="ACHAT0_000548"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8450517.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            3076..3351
                     /locus_tag="ACHAT0_000549"
     CDS             3076..3351
                     /locus_tag="ACHAT0_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8450518.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3357..4124
                     /locus_tag="ACHAT0_000550"
     CDS             3357..4124
                     /locus_tag="ACHAT0_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8450519.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4292..6121
                     /gene="typA"
                     /locus_tag="ACHAT0_000551"
     CDS             4292..6121
                     /gene="typA"
                     /locus_tag="ACHAT0_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8450520.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6382..8141
                     /gene="recQ"
                     /locus_tag="ACHAT0_000552"
                     /pseudo
     CDS             6382..8141
                     /gene="recQ"
                     /locus_tag="ACHAT0_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
     gene            8255..8557
                     /locus_tag="ACHAT0_000553"
     CDS             8255..8557
                     /locus_tag="ACHAT0_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8450521.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8560..9723
                     /locus_tag="ACHAT0_000554"
     CDS             8560..9723
                     /locus_tag="ACHAT0_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8450522.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9749..13177
                     /locus_tag="ACHAT0_000555"
     CDS             9749..13177
                     /locus_tag="ACHAT0_000555"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8450523.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13198..14322
                     /locus_tag="ACHAT0_000556"
     CDS             13198..14322
                     /locus_tag="ACHAT0_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8450524.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14303..14749
                     /locus_tag="ACHAT0_000557"
     CDS             14303..14749
                     /locus_tag="ACHAT0_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8450525.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14733..15071
                     /locus_tag="ACHAT0_000558"
     CDS             14733..15071
                     /locus_tag="ACHAT0_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8450526.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15190..15750
                     /gene="rsmD"
                     /locus_tag="ACHAT0_000559"
     CDS             15190..15750
                     /gene="rsmD"
                     /locus_tag="ACHAT0_000559"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8450527.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15747..16238
                     /gene="coaD"
                     /locus_tag="ACHAT0_000560"
     CDS             15747..16238
                     /gene="coaD"
                     /locus_tag="ACHAT0_000560"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8450528.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16228..17271
                     /locus_tag="ACHAT0_000561"
     CDS             16228..17271
                     /locus_tag="ACHAT0_000561"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8450529.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17430..18158
                     /locus_tag="ACHAT0_000562"
     CDS             17430..18158
                     /locus_tag="ACHAT0_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8450530.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            18274..18519
                     /locus_tag="ACHAT0_000563"
     CDS             18274..18519
                     /locus_tag="ACHAT0_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8450531.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18516..18734
                     /locus_tag="ACHAT0_000564"
     CDS             18516..18734
                     /locus_tag="ACHAT0_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8450532.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18870..19409
                     /locus_tag="ACHAT0_000565"
     CDS             18870..19409
                     /locus_tag="ACHAT0_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8450533.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19402..21675
                     /locus_tag="ACHAT0_000566"
     CDS             19402..21675
                     /locus_tag="ACHAT0_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8450534.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21713..22759
                     /gene="holA"
                     /locus_tag="ACHAT0_000567"
     CDS             21713..22759
                     /gene="holA"
                     /locus_tag="ACHAT0_000567"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8450535.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22848..23102)
                     /gene="rpsT"
                     /locus_tag="ACHAT0_000568"
     CDS             complement(22848..23102)
                     /gene="rpsT"
                     /locus_tag="ACHAT0_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8450536.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23502..23771)
                     /locus_tag="ACHAT0_000569"
     CDS             complement(23502..23771)
                     /locus_tag="ACHAT0_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450537.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(24033..24161)
                     /locus_tag="ACHAT0_000570"
     CDS             complement(24033..24161)
                     /locus_tag="ACHAT0_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450538.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(24241..24696)
                     /locus_tag="ACHAT0_000571"
     CDS             complement(24241..24696)
                     /locus_tag="ACHAT0_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8450539.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24834..26762)
                     /locus_tag="ACHAT0_000572"
     CDS             complement(24834..26762)
                     /locus_tag="ACHAT0_000572"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8450540.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26959..27792)
                     /locus_tag="ACHAT0_000573"
     CDS             complement(26959..27792)
                     /locus_tag="ACHAT0_000573"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8450541.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27984..28688)
                     /locus_tag="ACHAT0_000574"
     CDS             complement(27984..28688)
                     /locus_tag="ACHAT0_000574"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8450542.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28711..29337)
                     /locus_tag="ACHAT0_000575"
     CDS             complement(28711..29337)
                     /locus_tag="ACHAT0_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8450543.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29663..32038
                     /locus_tag="ACHAT0_000576"
     CDS             29663..32038
                     /locus_tag="ACHAT0_000576"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8450544.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(32062..32553)
                     /locus_tag="ACHAT0_000577"
     CDS             complement(32062..32553)
                     /locus_tag="ACHAT0_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8450545.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32740..33660
                     /locus_tag="ACHAT0_000578"
     CDS             32740..33660
                     /locus_tag="ACHAT0_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8450546.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33814..34158
                     /locus_tag="ACHAT0_000579"
     CDS             33814..34158
                     /locus_tag="ACHAT0_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8450547.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34348..34848)
                     /locus_tag="ACHAT0_000580"
     CDS             complement(34348..34848)
                     /locus_tag="ACHAT0_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8450548.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35376..35459
                     /locus_tag="ACHAT0_000581"
     tRNA            35376..35459
                     /locus_tag="ACHAT0_000581"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35410..35412,aa:Leu,seq:cag)
     gene            complement(35783..>36217)
                     /locus_tag="ACHAT0_000582"
     CDS             complement(35783..>36217)
                     /locus_tag="ACHAT0_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8450549.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11985 a         6325 c         7491 g        10416 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
     3001 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
     3061 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
     3121 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
     3181 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
     3241 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
     3301 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
     3361 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
     3421 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
     3481 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
     3541 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
     3601 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
     3661 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
     3721 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
     3781 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
     3841 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
     3901 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
     3961 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
     4021 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
     4081 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
     4141 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
     4201 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
     4261 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
     4321 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
     4381 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
     4441 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
     4501 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
     4561 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
     4621 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
     4681 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
     4741 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
     4801 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
     4861 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
     4921 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
     4981 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
     5041 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
     5101 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
     5161 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
     5221 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
     5281 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
     5341 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
     5401 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
     5461 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
     5521 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
     5581 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
     5641 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
     5701 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
     5761 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
     5821 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
     5881 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
     5941 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
     6001 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
     6061 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
     6121 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
     6181 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
     6241 gccgaaagac gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa
     6301 taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg
     6361 gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag
     6421 tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga
     6481 atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat
     6541 gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg
     6601 aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct
     6661 agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt
     6721 tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt
     6781 gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg
     6841 gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg
     6901 actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt
     6961 cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa
     7021 caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca
     7081 acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt
     7141 agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac
     7201 gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat
     7261 gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct
     7321 ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat
     7381 atccaagtat ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa tatgaaaaat
     7441 taaaagcgat gacagaatac gtgtatattg agtcctgtct tcagcaatac atcctaaact
     7501 attttggcga gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat gacagagaaa
     7561 tagtagaagt aacgacagaa gcacagatgg ttctttcttg cttaaaacgt atgggtgaga
     7621 actatggtaa acaaatgctg atgaaagtct tagcaggatc gaaagaacaa aagctgcaag
     7681 ctttaggttt tggacattta tctacctatg gactgatgaa aaaccaatcg caaaaagaaa
     7741 cgatgcagtt aattgaatat ttgatatcga atggctatct tctgaccatc aatggtgagt
     7801 atcctgtttt gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa gaatctgttt
     7861 atcgaaagga acccaagaaa gtccagcagc tatcggatga agaaaccgat acactatttg
     7921 aagtgctgcg ggaactgcga acggatctag catcagaagc aggcgttccc ccgtatgtcg
     7981 tattttcaga ttccacattg aaagaaatga gccgaatacg tccatcaagc agattggaga
     8041 tgcttcagat aaaaggagtt ggccaaagca agcttgataa atatggcgaa gcctttttat
     8101 ctcggataaa aaatgcggac tctaacgttc tggataagta gaaagaacga atacgtaaaa
     8161 atgaataaaa acaactggtt aaatctgttt tttttgctcc ttatgaaaat caaatatgtt
     8221 ataattaagg aattagaatg ggaggtaagc acagatggat tctttgtcga aaaaacttgc
     8281 gatccaagta ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa atcaaaacaa
     8341 cagtctctgt atctcacaat gcaaagcctt cgaagaagtg gtagatacac agatgtacgg
     8401 cttttcccgg caagtcggtt ttgctgtccg tataggagta atcggaaaga acgagggcca
     8461 gctgatgtta agtgagctag aacgagaatt gaatcagtta tatagtgagg tttaccaaga
     8521 aatttatgat aaaaaagaag ttggcaagga ggaatagact tgcctaagaa agtgaaaaaa
     8581 aggcatctat tagactatag catcttgatt ccctatttaa ttttatgtgt attgggattg
     8641 atcatggtat atagttctac gtcgtatcta ttattagaaa acggacaaaa tccgtccgca
     8701 tcagtcatta accagagtat tttttgggtg cttagcttga ttgctatcgc actactttat
     8761 aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca tggcagccat cgcagttttg
     8821 acgattttgc tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc gaaaggttgg
     8881 ctgcaaattg caggattctc gatccaacct gccgaatacc taaaaatcat ttctatctgg
     8941 tacctgtctc tgactttatc taaaagacaa aacagtgtac aaaaagattt tcttgggact
     9001 gtgaaacgtc cacttgcaat ggttatcgga cttactgcat tagtcgctat tcttcccgat
     9061 tttggtaatg cggcagttat ctttttgatt attttggttc tgcttttagc cagcggagtc
     9121 aattatgtgt atactttgat tgtaggagta ggaggctttt gtctaagtac ttttacgatc
     9181 tggctgatca atatcacaaa cggaaagatc ttacccggac gtttacagta tatctataat
     9241 cgatttgcta tttaccaaaa tcctttctca gacgaattaa ataaagggca tcaactagtc
     9301 aacggctact atgccatgtt caacggagga ctatttggca ggggattagg taacagtatc
     9361 cagaaaaaag gatttttaca agaagcacaa accgatttta tttatgcaat cgtagtggaa
     9421 gaactgggag tgattatggg gattttgata ttagcgttgc tattctttat gattgtgcgc
     9481 attgtccttg taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg cattgggatc
     9541 ggcgcaatgt ttttgatcca agtatttgtc aatcttggag gaattactgg cgttattcct
     9601 ttgacaggga tcacgttccc atttttaagt caaggaggct ccagtttgct gatgctttcg
     9661 atttgtgtcg gctttgtcct caacatcagt gcggatgaaa aacgcaagag cttaggttta
     9721 taataaaaaa agatgtagga gtggttcgat gaaaaaagtt ttagttgcta acaggggtga
     9781 aatcgctgta cgtgtatttc gtgcttgtac agaattagga attaaaactg ttggaatcta
     9841 tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat atttagtagg
     9901 acaaggaaaa aaaccgattg atgcgtatct agatattgaa gggatcattt ctatcgcgaa
     9961 agaatgcgga gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa acttgaattt
    10021 tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga cccaaactgc atcatttaga
    10081 tatttttgga gataaaatca aagcaaaagc tgcagctatc gaagcgggga ttgcttcgat
    10141 tccaggcaca gatggaccaa tcgcttctat agacgatgcc ttggagtttg cgaaacaata
    10201 cggttatccg attatgatca aggcagctct aggcggcggc ggacgcggca tgcgtgtagc
    10261 tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag caaaagcagc
    10321 ctttggtagt gatgaagtgt atgttgaaaa atacatcgct aatcctaagc atatcgaagt
    10381 acagattctc ggggatacgc atggaaacgt catccatctt tttgaaagag attgttctgt
    10441 tcagcgccgt caccaaaaag tagtagaagt agcgccttgt gtctctatga atgaacagca
    10501 acgtcaaaaa atctgccagg cagctgtcca attgatgaaa tatgtcgggt atgtcaacgc
    10561 aggtacagta gaatttcttg ttgaaggaga tgatttttac tttatcgaag tcaaccctcg
    10621 cgtccaagtg gaacatacga tcacagaaat gattacagat attgatatcg taacaacgca
    10681 actgttgatt gctcaaggtt tggatctgca taaagaaatc ggattgcctc agcaagaagg
    10741 aattaaatta aatggttcag ccattcaatg ccgaatcact acagaagacc cgttgaataa
    10801 ctttttacca gacacaggga aaatcgatac gtatcgttca cctggtggtt ttggcgtacg
    10861 tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg attcattgct
    10921 agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa tggaacgttg
    10981 cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt ccatttatgc tcaatgtcat
    11041 cacacatcct gaattccaat caggaaatgc aaaaacaacc ttcattgata gtacagcaac
    11101 attatttgaa tttcctcgac tacgagaccg cggaaacaaa acgatgaaat atatcggtga
    11161 aattactgtg aatggttttc caggtatcga aagtggcgaa aaaccatttt atgaagaacc
    11221 acgtatgcct aaagatctaa tcacaagaac tgattacgta acggctaaga atgtattaga
    11281 tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga caggaaaatt tgttgttgac
    11341 ggatacgaca tttagagacg cccatcaaag tctgctagca actcgtgttc ggacaaagga
    11401 tttcaagcag attgcacgtt tgactggaga aggtcttcct gaactctttt caagtgagat
    11461 gtggggggga gcaacatttg atgtggctta ccgctttttg aatgaagatc cttggcaacg
    11521 gttgagaaaa attcgttctt taatgcctaa tacgttgctg caaatgcttt tcagaggttc
    11581 aaatgccgtt gggtattcta attatcctga caatgtattg gtggaatttg tcaaagaagc
    11641 agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt ttgaactgga ctccacaaat
    11701 ggaaaaaagt atccaagctg tacgggatac tggtaaaatc gcagaggcag ctatttgcta
    11761 tactggcgat atcaacgatc caagtcgggc aaaatacaat gttcagtatt ataaagacat
    11821 ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca atcaaagata tggctgggct
    11881 gcttaaaccg caggcagctt atcgtttgat tagtgaattg aaagaaacga cagatctgcc
    11941 aatccatctg catactcatg ataccagcgg gaatggaatc atcacttatt ctgctgcttc
    12001 aaaagcagga gtagacatcg ttgacgtggc aatgagtgca atgagtggga atactagtca
    12061 gcctagcatg agcagcttgt attatgcact ggtcaatggt ccacggctgc cagaaatcac
    12121 gattgaaaat gcacagaaat taaaccatta ttgggaagat gtgcgcatgt attacaaacc
    12181 gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa tgccaggagg
    12241 acagtattcg aatctacagc aacaggcaaa agctgtcgga ttaggtcatc gctgggatga
    12301 aatcaaacaa atgtatcata ctgtcaactt gatgtttggc gatatcgtta aagtaactcc
    12361 atcatctaaa gtagttggag atatggcact atttatggtc caaaatgatc ttacagaaga
    12421 agatatttat gaaaaaggcg aaacactaag tttccctgaa tctgttgtga ctttcttcca
    12481 aggagaacta gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga ttattcttaa
    12541 aggcagacct gcattgaatg aacgtccagg tcttttggct gaatcagtcg acttcaacga
    12601 agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca aaacaagacg aagtattaag
    12661 ctatttgatg tatccgcaag tctttttgga ctatcaaaaa gcatataatc aatttggcga
    12721 tgttacatta ctggacacac caacattttt ccaaggtata cgtttgggtg aaacaatcaa
    12781 tgtacaaatc gaacgcggaa aaacgcttat cattcgttta gatgaaatcg gagaacccga
    12841 tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg aaatcactat
    12901 caaagataat tcgatcatca gtacggtcca aacccgattg aaagctgaac cgacaaaccg
    12961 agaacaaatt ggagcaacga tgtctggttc tgttttggat gtattagtga aaaaaggcga
    13021 taacgttaaa aaaggtgata cgttgatgat tactgaagcg atgaaaatgg aaacggccat
    13081 cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg tcttcaggag acactatttc
    13141 gtctggtgac cttttgattg aagtgactga gaaataataa gcgaaaggtg gggaaacatg
    13201 aaaagactgg gtttgttttg gctgattctg ctattagtta ttgtggcatt ctacatggaa
    13261 ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca aatatcaaac
    13321 caaatgacaa cgtggaaata ccaggagctt aaagcaactg gtttttctac ttatatcgga
    13381 aaaccgatcg atcagctgga aaaggatttt ggccaagcct atgatcgatt aactagcgga
    13441 tttggttttg aaacaagata ttatgaaaat aaagctagtc atctcacatt tgaagcgaac
    13501 atccaagaca gcagagtcac aactgttaaa gtactgcaaa cagggcctgc tgatatcgcg
    13561 ccgttttatt tagggatgac gatgcaagat cttacgaaaa tcactactat ctatactaat
    13621 ttcacttttg agtaccatga tacagaagtt ggaatcgaac taatggaaga ggatatgaat
    13681 tatcgtccgc taattgcttt tgataacgac acttttgcta ttttattttt tgatcaaaca
    13741 acaaacggcc tattttcagt tgtgtattta gataaagata gtcttttgaa actattgcca
    13801 tatcaagtgt ttggtgaagg ccttccacat tatcaagcag ataaatcggc tgattgggaa
    13861 tcaatcaatc gcgttaaaga acgaaaaagt acgactttat taaatatgct tagaagagaa
    13921 gacgaacttc ccatatacaa tcaaacactt gcctttcaaa accacagtca gctgctacta
    13981 catgattttt tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc tgattggcaa
    14041 aaatcgctag gcagtgctca aacgacagtg aaattctcac tgacgaatga tgaattggag
    14101 aagttaatca gtaaacaaaa attagaaaat actagtgggc tatttattca tccggcgatt
    14161 gacgctactt tttcgttttt atattggtac agcgatcctt acaaccatga ccgtttcatg
    14221 gacccgggaa cggattctgt aggcattgct ttttctaaag aaaatatgtt agtcttattg
    14281 caagaagaaa acggagaaag tagtgatagc agtgatcgtt aacgaagagc tgtttcaact
    14341 ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag agtaaaacga tggcttctta
    14401 tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt tgccagttga aagaagcgtt
    14461 cttgtctaag aaagaagcat ttgaaaatgt agcggcatat ggtgagtatg cacctgatta
    14521 tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag cttgatatgg atgagcatgt
    14581 tgcagctttc cgatatagtg aaacacagct gcaaagtctg ttggatcaaa ttgtccaagt
    14641 acttgccgaa gaagtttcta atgaaatcaa agtagatgca ggtagcccgt tttttgaaac
    14701 gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt agacaatagt gaatttgaaa
    14761 taacaaaacg acgcgggctg atcgtatggg tctacagctt aaaacaatta aagaatctga
    14821 aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa atatgtcgtg ctttacttaa
    14881 atgaagagac attggaagaa acagaagaac gaatccgtaa attacatttt gttcgaaacg
    14941 tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc cgaaaaaata ggaaacaaaa
    15001 cagtattaaa agacgaaggt ttcgaaattg aagagctaaa tacagaaatc cgattagctg
    15061 aacatcttta aaaatctctt atgagcagtt tttaaataaa aaataacttg gataacgatc
    15121 gatttttgat cattgtccaa gtattttttt ggtatactag taccagtacc atttctaagg
    15181 agtgagtaga tgcggataat atcaggaaat tatggcgggc gccgtttaaa aagtttagca
    15241 ggggcgaaca ccagaccaac tacagataaa gtaaaagaat ccatattcaa tatgattgga
    15301 ccatattttg atggagagac agtattggat ctgtttgcag gaagcggtgg tttagctatc
    15361 gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag acaaaaatta ccaagcgcta
    15421 aaaatcatca aagaaaacat cgaaattacg aaagaaccag agaaatttac cacaataaaa
    15481 cttgatgcgg acaaagcaat cgaacggcta gcagcggaga aacaacaatt cgactacttg
    15541 tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc aaatccataa aatggaagaa
    15601 ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa cggataaaac agtcgaattg
    15661 ccagaaactg tggctggatt ccatcaaata agaaaacaga tttatggcat aacggccgta
    15721 actatctata gaaaagaggg aaatcaatga aacgtgcatt attcccagga agttttgatc
    15781 cttttacaaa aggtcactta gatacggtgg aacgtgcggc taaattattc gatgaggtag
    15841 taattggtgt ttttatcaat acttcaaaga aaagtttgtt tcccccagaa gaaagaatga
    15901 cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa agtcatgcat caagaaaatc
    15961 aattgacggt agaaacagca aaagagattg gtgcagatgc gttgatcaga ggcatacgaa
    16021 gtatcaaaga ctttgaatat gaacgagaga ttgctcagat gaatcatcat ctatatgatg
    16081 aattggaaac tgtcttctta ttagcaaaac cagaatatag tcacgtgagt tccagtattt
    16141 taaaagaagt actgcacttt ggcggagatg tgagcagcta cttaccgcca gtgatcaacg
    16201 aagctttagc tggaaagcgt gaatcaaatg aaagctaaac aatggttgaa aacagtactg
    16261 attatcatat ttggaatgtt gatcattgga tttgtagtag ttcccttgcc gtattatgta
    16321 gaatcaccgg gagccacgat cgatctgaaa gaactaatta cggtcaatga taaggaagat
    16381 aaaggttctg ggtctttctc tcttacctct gttggtatca ggcaagctac aggtttctta
    16441 gctttaaaag ctaaaatgac cccttttgaa gaattgatca gtgaagaaga attgactggt
    16501 ggggcgacaa atgaagaata taatcaaatg caacagtatt atatggattc ttcccaaaat
    16561 gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt ataaaatgga attcaaaggc
    16621 gtatatgtca tgagtgtaga gaaaacatcg aatttctttg gcaagctttc agttggagat
    16681 acagtaacaa aagtagacgg gaaaagcttt caaagtacag aagcatttat ggattatgta
    16741 aaaagccaaa aagttggaca aacaatcgaa atcagttatt tgcataatgg tgaagaaaaa
    16801 acagcttctg gtgatctgat cgagttacca acagataaaa aggcaggaat aggtatcacg
    16861 cttactgacc ataccgagat cgaatcagat acagatgttc gtattaattc tggctctatc
    16921 ggtgggccat cagcgggatt gatgttcact ttagaaatct atgaacaagt cactggtaaa
    16981 aacttgcgac atggtaagca aattgcagga actggaacga tcaacagtga aggagaagtt
    17041 ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg ataaagctgg cgtagagatt
    17101 ttctttgcac cagatgatac gatttcagac gatgttaaga aagaatatcc tgaaataaaa
    17161 accaactacg aagaagcaaa agctgccgct aaaaaattga attcctcaat gaaaatcgta
    17221 ccagtaaaaa cagtacaaga agcgcttgac tatctagaga aaatgaaatg aaactaaata
    17281 attaaagagg ggttgtgaca cgagttctgt cacaacccct ctttttcgtc aaaaatcttc
    17341 acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta gatgttgaag ggacgatttt
    17401 gccagagctc actgtttctt caattttaaa tgaaaggagt aatgaatgat ttttactggt
    17461 cattctttga aaaaagaaca atgatagaaa aaatcagcaa gaaaatacta gcttcaccca
    17521 aattgatcat ttggatgtct atgatagttc ttatcttttg tatcgcaagt ggttttttat
    17581 tatcaaattt gctattttat agagaaacag aagagactgc tgcaaaagaa atactttctt
    17641 cgtttagttc cagtggagaa gatccatcag ctgaaggaaa cgatgtagaa gaggatgctc
    17701 gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa ggagcctggt atctattcct
    17761 tttcttcaga agaacgggtc tatgatgtac taaagcgtgc aggtggatta ttagaagaag
    17821 cggatagcga tcgaatcaac ttttctgcga agatagaaga tcagcaagtc ctatatatcc
    17881 ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt ggcatcctcg gaaggaaaac
    17941 agcctacagc agattccgag cctcccaaaa taaatatcaa tacagccact ccatcagaac
    18001 ttcagcagat tcctgggatc ggatcagtca aagcgcaaga aatcattcga tttagagaag
    18061 aaaacggatc cttccagaaa gtggaagacc tgcaggagat ttctggtatc ggtgaaaaga
    18121 cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa tgattcaaaa tcgactggca
    18181 cgagcaataa atggacgcta atggatacta agaataatcc aattctaaga gcttggcagg
    18241 acgaggaggt agtatttttg gataggaaat agcttgattg ttcgcaaaga aacattaaaa
    18301 aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg ggattcatat tatgaatacg
    18361 gctgtcagcg gcaataacag tcaagctcta cgagaacgct ttgccaaaga tgtacttagc
    18421 tatacagcag ataaagtatt tattctcatt gggacgaatg atctagcaga gcataaacaa
    18481 ctttctaaag aaacgtacca aaaaatctgc agtggatgat agttcagctg aaaaaagact
    18541 atagttctga tcatgctttc tttatcacgc caccagcagt cgatgagaca aaacaaaaat
    18601 atcggaataa caagttgatc aaagaatata tctctgttct cacagaagta gcaaaacgcc
    18661 atgattgtga agtatcgatt tttatacgga atttcttgaa caaatggatt cgaagaattt
    18721 ttataatggg ctaatggatg acggacttca tttcggcagt gaagggtagg atcttttgtc
    18781 agacaggatg attcgttata tttaaagaat attttgttta agcaatgagt actttttaat
    18841 caacttgaaa aaacggagga atataaaaca tgttagaaaa atcttcaaaa gcatgcgata
    18901 cgaataaaga aaaagttact gataagtcac ataatgaacg cattccttgg gaccagtact
    18961 ttatggctca ggcagtactg ttatctttac gtagtacgtg tacacgttta gaagtaggag
    19021 cgacactcgt tcgagaaaaa aggatcattg ctggcggcta taatggagca gtcagtggag
    19081 atgtccactg catagatgaa gggtgttacg tcgtggatgg acactgcttg cgaacgatcc
    19141 atgcagagat gaatgcactc ctacagtgtg caaaacttgg tatcccgacg gaaggttcag
    19201 aaatctatgt cacacatttt ccatgtttgg cctgtacaaa ggctttgttg caagctggtg
    19261 tgaaagggat caattatctc catgactatc gaaatgatcc ttatgcccaa gcgttgatcg
    19321 aacaattagg tgtatctgta caccaagttg ctttagactc acagcatttt tcaaaacttc
    19381 aaaatgaatt aacaggaaaa catgtctagc gttttagaaa ggcatgttcg aaatcggttg
    19441 atttttccgg caattacgtc gatgattatc tgtttgttgt attacactga caattggggt
    19501 tatcgtttgc ttcttgccgg aattttttta tggttttcgc taaaagaagg acgttttatc
    19561 gcgtacttgt gtattttgtg tagcataacg gtatccattt cttataggct acaagagtat
    19621 tcatttttag aggaaaaaac aacatctgcg caaattactg tatttacaga tacgattcgt
    19681 gcaaatggta attttttgac aatggaagga aggttatcag agaataaaca aaagctccag
    19741 ctttcttatc aagtaaacac agaagcagaa ttgaaaacat ggcttaactt ccgaggaaag
    19801 ggaatagaat tgactgctga aggcgagttc atctctccag caaaaaagcg gaatctttat
    19861 aattttgacc aagaagctta cttcaggaac catagaattt ctggacggtt ccaaattcaa
    19921 aagattgaac agatcaccat gcaaaatcac tggcgaaact ggcttcagag aaaaagagga
    19981 tcttttatct cctttgtcca atctgaattt ccgcaaaaaa caagtgtata tattaattct
    20041 ttattatggg gctataaaga tgcaggattc agagaaccag aagaaatttt tagggagagt
    20101 ggtttgctgc atctgttcag tctatcgggt ctacacatcc aattttattt agggtggctg
    20161 tattatttgt ttcgacgtat cggatggcca ctacacgtca gtattattcc gttgagtcta
    20221 ctaactatct gttatgtttc tattgcagga agttctattt ctgttttgcg tgctagtttg
    20281 ctgtttttat tgcgattgct agtcaaattg ctgaataagc attattccac catggactta
    20341 tttgcagttg tcttttggct tcttttacta tttgatcctc gatttctttt tcatgcgggt
    20401 ggacagctta gttttttcat gagttttttg tttatcctga tttcttttga tggagattgg
    20461 ttgaaaaaaa cggcctctca cgtcctgttt actatgatca cgatgccgtt gattgtatgg
    20521 catttttatg agtggcctat tttaggtagt ctttttacat tactgattgc accggtattc
    20581 aagtttcttt ttcttcctgg tgtgttattt ctcacactct tcaatcgctg gctgccggaa
    20641 actttacttt tgctgataga agagggatta atcttatttg agcgaataat cgatttttct
    20701 tcagggtcgt ctctcattat aggacgtttg ccccttgtgc tatctgggat gtatattttc
    20761 ggattactga tttgtatgga taggttgaaa gaacaacctg tgaaacactt gttttatgtt
    20821 ggtctttttt cttttatttt tttgactttg ccatttttac gcttttcttc aacgatcgct
    20881 tttatcgatg tcggtcaagg agacagtatc tttttgcaaa gtccttttcg aaaagaaacg
    20941 attttgatcg atactggagg tcagttacac tttgaaagag aaaagtgggc gcaaggtatg
    21001 atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa aaggtcaggg gataagtgta
    21061 atcgataaac tcatcttgac acacgacgat acggatcatg tgggagaatt acccacgtta
    21121 tctcagcatt ttactatcaa aaaaatttat atcggttggg gcgctggacg aaatgagcca
    21181 ttacaaaggc atttatctga agctcaaaaa aatggtactg agatcattga aatcaaacat
    21241 ggggacagga tcagcggtta ttatgatctt tatgtattga ctccttttga aaagggagag
    21301 ggaagaaatg aagattcgat tggtctatgg atggaatata acaataggcg ctttttattt
    21361 ttaggagatt tgaatcaagc aatggaaaaa caattgcttt tgatttatcc taatctgaaa
    21421 gccgatgtag tcaaattagg tcatcacggt agccgtacgt caagtaacac agattttctt
    21481 tcccatattg aagcaaaaca tgggattata tcctgcggcg taaataatcg ttatggtcac
    21541 ccgcacagcg aagtattgga gacactagaa gaaaatcaga taaaaacatg gcgaacggat
    21601 caacaaggaa tgatcaccta tcgctggcat ccagtttttc atcctcatgg gctagtggaa
    21661 acaatgatag actagtaatt ctcatctgct catggtagga taatttttga aagtggtgag
    21721 taatttgagt atacaagcag agttacaaaa aatacgtaaa gaaaaacttg catcttgtta
    21781 cttgatttta ggaacagaaa agtttctcca agatcaagtt cggacagaaa ttgtcaaaaa
    21841 aatccaatta gagggcgaag atgacttaaa ttttttaagt tttgacatgg aaaatgcaag
    21901 tttagatgag gtagtagctg aagcagagac gttgccgttt ttcggagatc aacggttagt
    21961 attcgtagag aatccttatt ttttaacagg tgaaaaagta aacaatggaa tcgaacaaaa
    22021 tactgattta ttgacggctt atttgaaaga accattagaa tcgactgtat tagtattctt
    22081 tgcgccatat gaaaagttgg atgaacggaa aaaagtaacg aaacaattga aaaaaacagc
    22141 aattatcatc aatgtccaac agttgaatga aaaagaagta cgtcaatatc tgatgaatac
    22201 cttagagaat tcctcaatca aaatggaacg aagtgcaatt gatttattct tgaggttgac
    22261 cgacttagat ttatccaaaa tgatgagaga gttacagaaa ttggtccttt atggacaaaa
    22321 tcaacagcaa atcacggtaa gagaagtaga agaattagtt cctaaaacgt tagaacataa
    22381 tatttttgac atgacccaat atattttaaa agggaagacg gaacaagctt tgcgtttata
    22441 cgaagattta gttatgcaag gggaagaaac aatcaaaatt aatgcgattt tgctttctca
    22501 attacgttta tttttacaga caaaatttct tgtaaagatt ggttatcagc aggcaaatat
    22561 tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt gccatgcagg aagtaaaaaa
    22621 attcgatgaa catttgctgg tacagttgtt tgatcaatta gttgagatgg attatcaaat
    22681 caaaacggga caaatcgaaa aagaattaag ttttcaacta ttcgttttgc gcacagggca
    22741 gttgttaaga aatcgataag aagaaagacg agggcaaagc atgcgggaag gtcgtttgtt
    22801 tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc gattctttta tttagcgatt
    22861 tttttgctta aacgagattt gtcgcggtta gctttgtttt tgtggattag accttttgat
    22921 tcagccatat caatagcttt aacagcttct ttatataacg catctacgtt gtcagcacct
    22981 gaagctacag catcttcaaa ttttttgatt gcagtacgca tagcgcttgt ttgagatgaa
    23041 tttttcacat tagcgttttc gcttgtacga acacgtttga ttgcagattc aatgtttggc
    23101 attagtttca cctccgataa cttaagttct tacgcgtgtt accgcataat tcaacattca
    23161 ttattatacc taaaggcagc gtgctttgca atatgaacat gacaagtttt tttccttaac
    23221 agtcttttat taggattttt tttcagtaaa aacggatagt acaagctgaa aaaaatcaaa
    23281 aagacgctat tgtgggaggc aatagcgtca aaccaaacta gatttttatt tatctattta
    23341 aacaaagttt gaatgggaat gtttggtact aagttattat ataacaaaga gaaccgtttg
    23401 caaagttttt tttgaaaagg gatacgattc cctgttgttc tttgtttttc tattaagaaa
    23461 ctgcagttat tttataacca gtatcctttg atcagtaaag gctattctgg tgtaccgatt
    23521 attttaatgt attcatcgat attttttatc aacatttttt ttatgaccaa ctggttccga
    23581 cggttccatt tgccacttac ttccagcaca tatttattta ggggcaaacg taagatatat
    23641 tttgccaaat agcgatcttt aagcaggcca ttgatcacac ggtcctcaag ctgcaagttg
    23701 atcccagtca gcatcattgg ctgttcttgt aaaacgtacg tatcaattaa aagaccttca
    23761 aaagtttcca caagcgtctt cctttctttt caaagtaacc tgaaaacagg cactaattct
    23821 tccattttga tattcgttga agtggatgcg atcattcttt cttttgtcca ctgtaatcta
    23881 gtataacaaa aaagagcaga agagccaaaa agaaaaaaga gcatcgaatt ctataggaag
    23941 tctcattggc acggcctaaa ttattacaaa acattgatta aaaaaagccc ctgcatcgca
    24001 ggagcatccc ctatatcgtt ttaataggtc aatcaaaaag cagtatcaaa cacattttgt
    24061 atcaaatcag gaaataatag ataagctaag cccagaatca aaagtaccgc tagaagaaac
    24121 cagccgattg ttcctatgaa atactttaat ttttcgaaca ttgtatcacc tcgttgtcag
    24181 tatatcacgg atcagtaaat tgggggtgtt aagaatctcc taaaatagac aaaatttgtt
    24241 ctattttcct aaagcaaata cttgtaagat acagtaagtg ataccagaag caatgaccgg
    24301 tccggcggcg attcctctta agaagacaac acctaaaatc gtgccgaaaa caagtgcaac
    24361 agtgatttgt ggatcagaag caatcaatcc tacacccttg gaagataaga cagccactag
    24421 cccaccgcaa aaaacagcta cccagccaat aggggattta aaggcttcta atagatcacg
    24481 tagaccgatt tctcctgttg cgatagggat tagaatagcc gctgtgatga tcgtcacccc
    24541 aatattgatt cctttgcctt gaagccaagg gaaaaaggaa cgggaggctg gtatgagctt
    24601 cataatcaaa acaaaagctg ccgctatcat cagactttga tttttagcta aaattgctac
    24661 aagtaaaata attcctaaaa aaagccaact ttccatgaaa tatattcctc actttcttcg
    24721 aaacaatcat aacatgtttt tagaaagtta caaaagtagg aattcatcct tcaagaccta
    24781 ttcataagct gtttttttca taaaataaac aaaatcataa aaaaacactt tgcttatttg
    24841 acagaagaca gccgcgcttt gtatagtgga caagacttac atttttttga agaatcagaa
    24901 agatcaggta aaggtcttag taaacgcaag ccattcagaa taaccaatat tgtgcttcct
    24961 tcgtgtccta caactccaag aggtagattt aaaaattgca ggaagttaga agcaatcaac
    25021 aagagaataa caagaatcga aaaaataata ttttgtttga tgatccgccg catttttaaa
    25081 gatagccgat gactgtagac aaatttattt aagtcgcttt tcattaagac gatgtctgcg
    25141 atatccatag cggcatctgt cccttgcccc atagcgacac ctaaagaagc ggctgctaaa
    25201 gcaggcgcat cattgactcc atcaccaacc atcgcattta cttggtaaag ttcattctct
    25261 tttttgacta gttgtgtttt ttcttccgga gtacagcttg catagtaatg ttcaatccct
    25321 acttgttcag caatggcctt tgctgtgcca gaatggtcgc cggttatcat acttgtatgg
    25381 atgttttggg atttaaagta ggaaattact tgttgggtat ttgcttttgg aatatctagc
    25441 agacctaaaa cagcaattaa ctgctgatct ttagaaagat aaatcacggt tttgccttga
    25501 ttttccaaac gttcgatcat ttccgcaatc tctttgctga ttatcatcgt ctcaggatca
    25561 tatgcgtgct tgccaacttt ccattgcgca cctaaatagg tagtttgtaa tccaaaacca
    25621 gtaatttctt ctattggtaa gtattggatt tcttcaggta ttgtgcaatc gaaatattgc
    25681 aaaatcgcat gagcaagtgg atgcgtcgtt ttttgttcca tagcaacaag aagttgttgt
    25741 gccaaatcct tttcatcaaa gaaaaagctg tcagttacaa ctggttttcc tttagtgagc
    25801 gttcctgttt tatcaaaggc aatcgctttt aattcagaaa gcttttctaa atggataccg
    25861 cctttgatta aaatcccatt ttttgcagaa tttgaaagag cagctagaga agctggtgta
    25921 gcagatgcga caagcgcaca aggagaagcc acaactaata ataccattcc tcgataaaag
    25981 ctttcatccc aactccaacc taggaaaaag tgggggagaa ggatcattag aggaacagca
    26041 agcaaaatca attttacata attattttcg attcgttcga taaatgaagc tgtttgagtt
    26101 ggcgtattct gcgcttcttc tactaattgg ataatcttag caaagacagt ttcggaactc
    26161 gtttttgtta ctcgaatcgt gatgggattt ccttgattca gcgttcctgc atacatctca
    26221 tcgcctaaat atttttctac aggcacagat tcaccgctta atgcagactc atcgatcgtt
    26281 gcctgtgtgc tttctaaaaa gccatctatc ggaacagctg ctcctttagc gacaaatacc
    26341 aagttatcca cctttagttg agaaacatct acttcttgca tttgtccatt ttcttttaat
    26401 aattgggctt tttgtggttg aagattcatc aaagcagtaa tttctttttg acttttatta
    26461 gttgtgtatt cctctaatgc accgcttaaa caaaagatga atgtcaacat tgctccttca
    26521 aagtaattac cgatcaaaca ggcaccaata gcagctaaag ccatcaataa atccacattc
    26581 agtgtccgat cattccacaa ttctttcaag ccttccgatg tttgtttata tccaccgatg
    26641 ataatggcac tgatataaaa aagtggggtg atggtaagag agaatcggtc gaagataaaa
    26701 ccgaagatca taaataaaag gcagccgatt gttgctgcaa aagctttttt ctgattagtc
    26761 atcgttatta gacctccttg tcaaaacaag gatagcataa taaaaatgta attgataata
    26821 ataacagcta aatgagaacg ataatggtta tcatttggtg attaagcttg atgttgtttt
    26881 tattatttat aatgattatt attaaagaaa ataaaaaacc agaacatttc tcaaataaga
    26941 gaagtgttct ggttttttct acattgcaat aaaccaataa ccaatcaaaa tgataccaag
    27001 gataatacga taccagccaa aagcggtaaa gtcgtttttc ttcaaatagc ttagtaagaa
    27061 tttgatagct aagacggaaa ccacaaaaga tacgaatgtt cctacaagaa gaacaacagt
    27121 ttcttccatt ttgaatgaat tcccttcagc taaatatttg acgatcttca agccgcttgc
    27181 gccaaacatg acaggaatcc ctaagaaaaa tgaaaactct gtagcgacaa aacgcgaagc
    27241 accaatcaag atagatccta ggatcgttgc gccagaacgg gatgttccgg gaacaagtgc
    27301 caagacttgg aataacccaa caagaagagc tgcttggtac gtaaagtcat tcagagaagt
    27361 ccaacgaggt gttttgtttt tgttgcgttt ttcgacaata ataaaggcaa taccgtaaac
    27421 gatcaaaaca atggcaactg gtaagaactt gtggaaattt gcttctaacc aatcatccaa
    27481 aggcaaacca atgatgatgg ctggtatgca agcaacgatc actttaaacc aaagtgtcca
    27541 cgtatctttt ttttctaccg ttgatttact tggagaaaat gggtttaact tgtgaaaata
    27601 aagaacgacg acagccaaga tcgcccctaa ttggataacg acattgaaca tcgacataaa
    27661 ttgtgaactg gcatccagtt tgatgaattc atctgctagg atcaaatgac cagtactact
    27721 gatgggcagc cattccgtta ttccttcaat aataccgaga atcactgctt tgataatatt
    27781 tgcaaaaaac ataaattcct tccttcccaa ttaagataca tcttatagta taccgttatt
    27841 ttttgagaaa cccaatctgt tttttaatat taagaaaaac ttgagaaata aagcagataa
    27901 gtacacgaag agaaaaaaga agacaaagcc ctcgttcaga ctttgccttt tatgattctc
    27961 tggtaaaaat taaaatatcc ccattataga aacgagtcgc gactatgtgt tttcccgtta
    28021 tcgatataag acgtatcatt gatgccgtgg atagaaaatc gaccgtttgc ataagtgacc
    28081 tttgttacgc tgccatttaa aagcccagca cgaacaggct gtttaggatc gatcagatta
    28141 agcaaaaagg cgatcgtcaa accatgagat acgactaaaa catttccacc accatttttt
    28201 tcgcagtgat gggcgatatc ttcaaatcct gtccatacac ggtctcgcaa tgtttcataa
    28261 gtttccgccc acccagcggt atctaagttg tatacagcat tagcaatttg ttcaaaagaa
    28321 acttctgtgg taaacatttc atcatatgtt ttaaaattca atacacgagg gatcacaccc
    28381 cacatttcca tgtcatatcc accttcaaga gacccgaaac accattctct aatccttttt
    28441 tcaataaaat aaggaatttc ttttccctta gaatgttctc ctagaataat acgggcagtt
    28501 tcgattgcac gaccactgtc actagaataa gcaaatgaaa agtcgacatt ttttaacccg
    28561 cgacctaaat aatgaatgcc ttcttgtcct tgctttgtga ggggtgtatc acaccagcct
    28621 tgtactcgtt caattgtatt gaacatcgtt tttccgtgtc gtacgatata aagtgtagtt
    28681 tctgtcattt tattctcctc ctaagtcgtt ttaacgattg aaaaaaggat ttgtcctttt
    28741 ttcctgttca atcgtcgttg catcgccatg tcctggatag actgcaaatt catcaggtaa
    28801 tacaaacagc tgggtccgaa tactatgaag taattgttct aaattccctg tatacagatc
    28861 ggttctaccg atacttcctc taaacagggc gtcgccagaa acgacaaaat catcaaaaat
    28921 aaagctgacg cttcctatag aatgccctgg ggtagggacg acgcgaaaag acatgtttcc
    28981 taaagtataa gttttcattt caaattcata ttctgcaggc gagacaataa catcagcaat
    29041 atcatcatgt ctaccgagtc cagaaagatt caaaatcgga tcacctagcc atttttgctc
    29101 tagtgggctg acataaactg ggatttgata atacgcgcgt aaaggttcca ctgcaccgat
    29161 atgatcgtag tgagtatggg taagcaaaat cgctaccggc tgttggtttg tttttttgat
    29221 ttcttgtttg attcgttccg catcttctcc agggtcaatg atcaaaagtg cttcttcgtt
    29281 gtagaccaaa taacagtttt cttcaatcat tcctgttttt aatctttcga tatgcatgtg
    29341 cattcctcct tgatagccaa aacttctttc atccattagt ataacgcatt cattgctagg
    29401 aaacaaaaac aggtacttat ttctctttac ttatctgaat tatcagacta tatgaaaagt
    29461 atttggatat tctaaagaga aggaaagtaa gttttcatag cattttaata tgtccttgtt
    29521 acattagatt catccaaaaa tccaaattct atatcctata tccctgccta atcagcaggg
    29581 tgttttttta tgtttggaaa gagagctctt tttgctaaac taggtttttt caggtaatct
    29641 tagagagaaa gaggtggtgg aaatgatcaa acagtcaaaa atatctgtgc ggcacttagt
    29701 agaatttatt ttgcgacgtg gaagtatcga caatcgtaaa aaaagtaacc atactgcatt
    29761 agaaggagcg agaatccacc gcaagctcca aaaagatgcg ggagaaaact atgaaaaaga
    29821 agtcttttta aaaacaacag ttcaattaga tgaatattac ctgatcgttg aaggccgtgc
    29881 agacggtatt ttcaaaagag acggtattta ttatatagat gaaatcaaaa cctccgaacc
    29941 acgatttgaa gatttagaac cagaacaagt ggaacttttc tttcatcaag caagggtgta
    30001 tgcctatatc tatagtcaag aacatgattt acaagaaata aatctgcaat tgacttactt
    30061 ccagacggca gaagaagtga tcacgagaaa ggttgaacat cagacaagag agcagttaga
    30121 aacttttttt aaaaagctga ctgaagatta taaagaatgg cttgtttttc aagaaaactg
    30181 gcgcactgta cgcaatgcct ctttgatggc tttgaaattc ccccatgatg agtataggaa
    30241 aggtcagcgt gaactagcgg tagctgctta taaaacgatc cgtacgaaac agaaattgtt
    30301 tgtagaagca cctaccggaa caggaaaaac aatctctacg ttattccccg ctttgaaagc
    30361 tgtaggggaa gaagagggag aaaagatctt ttatctaacg gctaaaacaa taactagaca
    30421 agtagctgaa gatgctatga cagcattaaa agatacagga gctgaagtaa aaagtgtcac
    30481 tttgacggcg aaagacaaga tctgcttttt agatgagacg acctgtaatc cagatcagtg
    30541 cccatatgcc aatggttatt acaatcggat taatgaaggt ttatgggatc ttttgaatca
    30601 tgagaatcag atcactcgtg aagtcatcga aacttatgca agaaagcata cactttgtcc
    30661 tttcgaacta tcattagatg tcagtatctg gtgtgacgta atcattgggg attacaatta
    30721 tttatttgat ccaactgttt atttaagacg tttttttgaa gatgaaaaaa acgaggacta
    30781 tctgtttttg attgacgaag cacataattt ggtgaatcgt tcccgagaga tgtattcggc
    30841 agaactatct tatgaaaaaa cgaaacgaag taaggaagct ataccaaagg aattcaaaaa
    30901 acttcatcgc cgtttcaaca aactattaaa agaatttgat tcgatccgag aaatagcaaa
    30961 agaagatcat tgggattatc atcatcaaaa agcaccggct gaatctttag taaaggcagg
    31021 ttatcaatta agcgaaaaaa ttaaagaatg gctggcagaa tttcctgagc acccatcaca
    31081 agaacaattg cttccttatt attttgattt gcttcatttt ttgaaggtca gtgaatttta
    31141 tgatgatcac tacgaaacaa cagtcgaaaa aacgtatcgc gatctgatag taaaagagtt
    31201 ttgtattgat ccaagcttgt ttcttgagca gtcgttggat aaaggaaaaa gcagtattct
    31261 tttttctgct agtttttcac cactctctta ttatcaggaa acactggggg gacaaaaaag
    31321 tctggcttac aaactgccga gtcctttccc agaaggaaac cagcaagttt tcatcgcaaa
    31381 ttacctagag acgacttatc gaaaaaggga acacagtctg gcaaaattag tagagacgat
    31441 tcatgctttt gctgatggaa aggtcgggaa ttatttcgtt ttctttcctt cttatcaata
    31501 tttagatctt gcggtggaag cattcagaca gcaatatccc gacagcaatg tgctaatcca
    31561 agaaacggac atgaatgagg aagaacgcga acgattttta acaaaatttc aaacaaatcc
    31621 cgctgaaaca ttgattggat tttgcgtact tggcgggatt ttttcagaag gaatcgattt
    31681 gagaggggac cgattgatcg gcagtatgat cgtaggtgta ggtttgccgc agatgaatca
    31741 tgagcaggaa ctgatcaaat catattatga tgagaaggaa caccttggat ttgcttacgc
    31801 ttatcagctt cctggaatga acaaagtctt gcaagctggt ggtcgagtga ttcgtggcat
    31861 ggcggatcaa ggaatcgttg tattggcaga tcaacgattt tctagttttt cttatcgacg
    31921 tttattccca caacattggc aaacagcaag agaagtacga agtgttgggg aattggaaga
    31981 aggaatacag cgtttttggt tatcaaaaga agcagtaaat caaaaaaacg aggaatgaca
    32041 gacgtcagcc tcgttttttg attaacgata gttagtgaat tgcaactcga ttggtaagtc
    32101 aagtttacgt aataaagcaa tcacagcttg tagatcatca cggttcttac ctgtcactct
    32161 aatctgatct tcttggattt gtgcttttac tttcagtttt tcatttttga tagctgtagt
    32221 gattttcttc gcgttttcac gatcgattcc gttgaccaaa tcggcagttt gtcgagcttt
    32281 gccacctaga gcgtgttctg attcggaaaa atggatattt ttgattggca catttctttt
    32341 gatcagctta ccaaataata catctttgat ctgttcgatt ttgaaatcat catcgctcaa
    32401 tacaactaga ttgctgttct ctaatttgat ttctactgta gaatccttga agtcaaagcg
    32461 attggacaat tcttttgttg tttgctgaat cgcatttttt acttcttctg tattgacttc
    32521 tgatacaatg tcgaagctag catcttttgc cataggaacc ctcctgatat acatataaat
    32581 atttaccata catctatctt atcataggat tagggcagat tcttacctgt ttgctaaaat
    32641 tcttctcatt tgagttcgaa gtattttatt ttaatagata aactgttatt cttacatttg
    32701 gaataccaaa gtaggggaga ggtgcttgtg agtaaaatta tgactgcgta tcataaaaat
    32761 gaaagattaa agaaaatagt ggttattttg ataaccggat tgaccgctgc ggtcgcttta
    32821 aattattttt tgattccagc caatgtcttt tctgcaggga tgaatgggat cgctcaaatc
    32881 atttcctcgc ttttatatga atggtttcat atcaagatag acacaggaac atttattttt
    32941 cttttgaata tccctgtgtt tgtattaggg tttctcaaag taggaaaacg agcaacgatc
    33001 cttagttttg tgaatgttgt atgtgtatcg gtgatgacga tgctgcttcc agtcggacag
    33061 atcactgata atattttgat gaatgcccta gtaggcggtg ttttattagg tcttggcgta
    33121 ggattatctt taaaaatggg ctttacaact ggtgggatgg atatcatctc tcttgtactt
    33181 tcacaaacaa ctggaaaaac agtcggaaac tacatgctga ttctaaatgg tatcattgta
    33241 gtcatcgcag gcttcttctt taactgggaa agtgccttgt atacgattat ttccatttat
    33301 gcaatgactc aagtaattga tgccatccac acgagtcatc aaaaagtaac agcaatgatc
    33361 gttaccgtca agccagaaga ggtaacagaa gaactagcaa aacggatggt acgtggaatg
    33421 acattactgc cgtctgtcgg tggctattca aaaaaacaag ggaacatgat catgatggtc
    33481 atcacccgct acgaaatgta tgatcttgat cagatcgttc atgaaatcga cgacgatgca
    33541 tttatcaata ttttaccaac tcattcagta tttggccggt ttgcaaatga aaacgaacaa
    33601 aaaatgtatc gaacaactgg cgtattgcca gaaataaaaa atcgttcaca aaaaaaatga
    33661 ggaatcaggt ttattttaga ataaatttaa tctattacat ctaataagaa catttttata
    33721 aatttaaacg agaatcttac ttctaaattc tcatttacaa gcagctaaaa aaagagtatg
    33781 ataaataaaa atcgtgttaa ggagtgtcgt acgatgagcg aagcaaacca agaacggtct
    33841 ggacaagaaa tagaactaat caaagaacgt attcttgctg ccttggaaat ggtgattgat
    33901 cctgaattag gaatcgacat cgtcaattta ggcttgattt atgatataga attcaatcct
    33961 gaaaatggag aaacagtcat taagatgact ctaacaacta tgggctgtcc attagcagat
    34021 attttaacag aatctatcca tgacgcatta aaagaagtgc cagaagtaac gaaagcagaa
    34081 gtcaaactag tctggtatcc agcatggacg acagataaga tgagccgtta tgcacggatt
    34141 gcgttgggga tacgatagaa atcttggctg actagctatg agaagcaaat aggaaaaaca
    34201 aaaaagcctt ttatcctttc tagaagaaaa gagataaaag gtttctttgt ttataaaatt
    34261 ttgtctgcta tattataagt taattaaata aatgtgttct attgataaag gacattcaaa
    34321 tccctttcct tttggaatcc ttctttttta catcagtttg attttcgtat agtattcttt
    34381 ctcatcttca cgataaatcg cagccccatt tttttgttgt gctttaagac tgccaagatt
    34441 ttttttattt acagacaaaa gtgcctcatc aatgccgatt ttttttgcct cttgtagtac
    34501 taatgctaat ccttgtgtgg catatccttt tttacgataa ttaggggaga tgccgtaacc
    34561 aatatggcca gcgccgtttc ttaatgtttc gttcagttta tggcgcaaat taaaaattcc
    34621 tacatactca tgttcatcac tttctaaaat aaagacggtt gcgggaacgt aactctcagg
    34681 aagatcgatt cctaaagacg ttttttcgca tttttttaca taatctaaaa attcagcaaa
    34741 tgtatagcca taagcattgt tgataaatcc actctcattt ggtccaaagc tagtttgtag
    34801 ctgataagct ttctttaaat ctgcttccca tagtttgatc agtttcatcg tattcacctc
    34861 tttaagtaac cttattcatt tgttttctac tatagcacta ttgttgtaaa tagccatgcc
    34921 tatttttatt tattgtgtca tatcgtatta agaatcatcg aacaatacaa atatgaatta
    34981 caataaaaaa attatttgaa gttcgttcat tcgttactta tgatctctag aagaaagaaa
    35041 acgtgaatta cttcagcgtc tagaattcca gaaggatagt aatcaacaga agaaataatc
    35101 aatatccaaa tgaaagaaga tagctgaatc gagaaaaaga ggaaaaaaga aatgaagaaa
    35161 aaaataaaat agttactaat cccagagtcg ttcgtttaac attgaaatcg atcatatgga
    35221 taaaacagat tttttcacca accctcaata gatgatggag ttgctcggat gaattatttg
    35281 tgtagatggc ataagggagg gcgtacagaa atgaaaaaga acaaaaatat gttcttgaca
    35341 aaaaacacat gtaatcctaa actaaaagat gtgatgcggc tatggcggaa ttggcagacg
    35401 cgctagcttc aggcgctagt gagggtgacc tcgtggaagt tcgagtcttc ttagccgcat
    35461 aatttgtttt tttataaaga gaaggtacac ctggagcgat gagaaagaaa ataacatatt
    35521 atctaaaaga atataggaaa aattttaaac aacggaacca gattcaaata aatcaaaaaa
    35581 aatttgtgtg aaggtgggac agtttaaaac ctaatagatt gattcaaata gtagatttat
    35641 agattctgat acaagaaata ctggcaaacg atagttaagg aataaagact aaacttaaac
    35701 tcaaaaagag gtcatgcgaa agaataaatc gcatgacctc ttactatatt ttgaatgtgt
    35761 ttaaacgttc aaataatgaa tcttagtacc aaccgtttgc ttcccagaat gctttcgccg
    35821 cttcccaaga accataacgt gaagtaacat attgttctgc tactctttct tggtttgcag
    35881 cagagtagtc accatttaag taagaagaat ctaattggta acggccgatg taacgtccgt
    35941 tcgttgctgt gtaagaaccg cttgattctt tttgtgcgat ccattctttt gcagagcttg
    36001 tacttgctgg agtagcagca gtttctgtta cagcaggagc aggttgtgtt tcagcaacag
    36061 gcgctgcttg tgtttccgtt tgaaccgttt cttgtacaac aggttgttca gcttgaacag
    36121 gttcagcttg gactggtgct tcttgaacgg ctgcttgagg ttcagcagct gcttgtgcag
    36181 agccttctgt tgggattgtt aattgttgtc ctgagta
//
LOCUS       DAYYAR010000014        35643 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000014 DAYYAR010000000
VERSION     DAYYAR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.014"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            324..2090
                     /locus_tag="ACHAT0_000583"
     CDS             324..2090
                     /locus_tag="ACHAT0_000583"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8450550.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2192..3451
                     /locus_tag="ACHAT0_000584"
     CDS             2192..3451
                     /locus_tag="ACHAT0_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8450551.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3598..4899
                     /locus_tag="ACHAT0_000585"
     CDS             3598..4899
                     /locus_tag="ACHAT0_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8450552.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4902..5753
                     /locus_tag="ACHAT0_000586"
     CDS             4902..5753
                     /locus_tag="ACHAT0_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8450553.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5766..6581
                     /locus_tag="ACHAT0_000587"
     CDS             5766..6581
                     /locus_tag="ACHAT0_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8450554.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6732..7898
                     /locus_tag="ACHAT0_000588"
     CDS             6732..7898
                     /locus_tag="ACHAT0_000588"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8450555.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8128..8775
                     /locus_tag="ACHAT0_000589"
     CDS             8128..8775
                     /locus_tag="ACHAT0_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8450556.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8859..9803
                     /locus_tag="ACHAT0_000590"
     CDS             8859..9803
                     /locus_tag="ACHAT0_000590"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8450557.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9938..11389
                     /locus_tag="ACHAT0_000591"
     CDS             9938..11389
                     /locus_tag="ACHAT0_000591"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8450558.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11410..12246
                     /locus_tag="ACHAT0_000592"
     CDS             11410..12246
                     /locus_tag="ACHAT0_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8450559.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12246..12905
                     /locus_tag="ACHAT0_000593"
     CDS             12246..12905
                     /locus_tag="ACHAT0_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8450560.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            13031..13720
                     /locus_tag="ACHAT0_000594"
     CDS             13031..13720
                     /locus_tag="ACHAT0_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450561.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13726..14682
                     /locus_tag="ACHAT0_000595"
     CDS             13726..14682
                     /locus_tag="ACHAT0_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8450562.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14726..15238
                     /locus_tag="ACHAT0_000596"
     CDS             14726..15238
                     /locus_tag="ACHAT0_000596"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8450563.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15494..16042)
                     /locus_tag="ACHAT0_000597"
     CDS             complement(15494..16042)
                     /locus_tag="ACHAT0_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8450564.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16131..17312)
                     /locus_tag="ACHAT0_000598"
     CDS             complement(16131..17312)
                     /locus_tag="ACHAT0_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8450565.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGLGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17548..19056)
                     /locus_tag="ACHAT0_000599"
     CDS             complement(17548..19056)
                     /locus_tag="ACHAT0_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8450566.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19215..19406
                     /locus_tag="ACHAT0_000600"
     CDS             19215..19406
                     /locus_tag="ACHAT0_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450567.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19609..20526
                     /locus_tag="ACHAT0_000601"
     CDS             19609..20526
                     /locus_tag="ACHAT0_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8450568.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20519..21592
                     /locus_tag="ACHAT0_000602"
     CDS             20519..21592
                     /locus_tag="ACHAT0_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450569.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21740..22258
                     /locus_tag="ACHAT0_000603"
     CDS             21740..22258
                     /locus_tag="ACHAT0_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450570.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22239..22631
                     /locus_tag="ACHAT0_000604"
     CDS             22239..22631
                     /locus_tag="ACHAT0_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8450571.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22707..23642
                     /locus_tag="ACHAT0_000605"
     CDS             22707..23642
                     /locus_tag="ACHAT0_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8450572.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23775..24554
                     /locus_tag="ACHAT0_000606"
     CDS             23775..24554
                     /locus_tag="ACHAT0_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8450573.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24547..24864
                     /locus_tag="ACHAT0_000607"
     CDS             24547..24864
                     /locus_tag="ACHAT0_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8450574.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25108..26718
                     /locus_tag="ACHAT0_000608"
     CDS             25108..26718
                     /locus_tag="ACHAT0_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8450575.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26875..27723
                     /locus_tag="ACHAT0_000609"
     CDS             26875..27723
                     /locus_tag="ACHAT0_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8450576.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27954..28433
                     /locus_tag="ACHAT0_000610"
     CDS             27954..28433
                     /locus_tag="ACHAT0_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8450577.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28466..30694
                     /locus_tag="ACHAT0_000611"
     CDS             28466..30694
                     /locus_tag="ACHAT0_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8450578.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30823..32466
                     /locus_tag="ACHAT0_000612"
     CDS             30823..32466
                     /locus_tag="ACHAT0_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8450579.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32539..33084)
                     /locus_tag="ACHAT0_000613"
     CDS             complement(32539..33084)
                     /locus_tag="ACHAT0_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8450580.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33107..33592)
                     /locus_tag="ACHAT0_000614"
     CDS             complement(33107..33592)
                     /locus_tag="ACHAT0_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8450581.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33582..34394)
                     /locus_tag="ACHAT0_000615"
     CDS             complement(33582..34394)
                     /locus_tag="ACHAT0_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8450582.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34533..>35643)
                     /locus_tag="ACHAT0_000616"
     CDS             complement(34533..>35643)
                     /locus_tag="ACHAT0_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8450583.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11669 a         6409 c         7117 g        10448 t
ORIGIN      
        1 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
       61 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
      121 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
      181 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
      241 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
      301 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
      361 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
      421 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
      481 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
      541 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
      601 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
      661 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
      721 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
      781 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
      841 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
      901 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
      961 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
     1021 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
     1081 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
     1141 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
     1201 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
     1261 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
     1321 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
     1381 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
     1441 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
     1501 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
     1561 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
     1621 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
     1681 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
     1741 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
     1801 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
     1861 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
     1921 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
     1981 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
     2041 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
     2101 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
     2161 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
     2221 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
     2281 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
     2341 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
     2401 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
     2461 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
     2521 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
     2581 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
     2641 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
     2701 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
     2761 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
     2821 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
     2881 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
     2941 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
     3001 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
     3061 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
     3121 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
     3181 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
     3241 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
     3301 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
     3361 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
     3421 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
     3481 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
     3541 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
     3601 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
     3661 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
     3721 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
     3781 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
     3841 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
     3901 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
     3961 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
     4021 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
     4081 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
     4141 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
     4201 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
     4261 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
     4321 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
     4381 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
     4441 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
     4501 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
     4561 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
     4621 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
     4681 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
     4741 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
     4801 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
     4861 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
     4921 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
     4981 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
     5041 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
     5101 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
     5161 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
     5221 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
     5281 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
     5341 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
     5401 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
     5461 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
     5521 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
     5581 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
     5641 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
     5701 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
     5761 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
     5821 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
     5881 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
     5941 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
     6001 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
     6061 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
     6121 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
     6181 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
     6241 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
     6301 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
     6361 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
     6421 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
     6481 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
     6541 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
     6601 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
     6661 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
     6721 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
     6781 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
     6841 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
     6901 cttctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
     6961 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
     7021 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
     7081 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
     7141 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
     7201 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
     7261 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
     7321 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
     7381 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
     7441 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
     7501 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
     7561 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
     7621 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
     7681 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
     7741 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
     7801 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
     7861 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
     7921 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
     7981 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
     8041 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
     8101 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
     8161 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
     8221 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
     8281 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
     8341 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
     8401 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
     8461 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
     8521 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
     8581 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
     8641 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
     8701 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
     8761 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
     8821 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
     8881 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
     8941 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
     9001 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
     9061 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
     9121 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
     9181 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
     9241 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
     9301 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
     9361 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
     9421 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
     9481 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
     9541 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
     9601 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
     9661 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
     9721 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
     9781 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
     9841 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
     9901 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
     9961 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    10021 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    10081 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    10141 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    10201 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    10261 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    10321 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    10381 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    10441 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    10501 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    10561 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    10621 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    10681 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    10741 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    10801 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    10861 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    10921 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    10981 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    11041 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    11101 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    11161 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    11221 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    11281 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    11341 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    11401 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    11461 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    11521 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    11581 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    11641 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    11701 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    11761 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    11821 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    11881 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    11941 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    12001 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    12061 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    12121 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    12181 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    12241 gagtgatgta ctttcctcta attgattttt ccctcatgtg gacttcactt cctttcgtac
    12301 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatctgg
    12361 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    12421 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    12481 ttgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    12541 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    12601 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    12661 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    12721 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    12781 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    12841 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatg catcagtcaa
    12901 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    12961 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    13021 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    13081 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    13141 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    13201 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    13261 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    13321 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    13381 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    13441 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    13501 aaaatatttt ccaacagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    13561 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    13621 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    13681 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    13741 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    13801 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    13861 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    13921 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    13981 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    14041 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    14101 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    14161 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    14221 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    14281 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    14341 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    14401 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    14461 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    14521 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    14581 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    14641 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    14701 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    14761 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    14821 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    14881 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaagcgt
    14941 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    15001 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    15061 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    15121 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    15181 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    15241 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    15301 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    15361 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    15421 ccttcatcga gaggtcgtga catagctttt gtcacgacct ctcggcttat cctacgagta
    15481 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    15541 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    15601 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    15661 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    15721 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    15781 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    15841 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    15901 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    15961 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    16021 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    16081 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtata ttataccagc
    16141 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    16201 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    16261 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    16321 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    16381 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    16441 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    16501 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    16561 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    16621 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    16681 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    16741 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    16801 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    16861 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    16921 cccaacccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    16981 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    17041 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    17101 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    17161 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    17221 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    17281 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    17341 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    17401 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    17461 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    17521 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    17581 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    17641 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    17701 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    17761 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    17821 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    17881 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    17941 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    18001 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    18061 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    18121 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    18181 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    18241 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    18301 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    18361 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    18421 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    18481 atgatacagt tggttcaagg aagtagcgag ctttccactt cctgggccag gagctgtcac
    18541 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    18601 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    18661 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    18721 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    18781 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    18841 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    18901 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactagct tcccaccaaa
    18961 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    19021 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    19081 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    19141 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    19201 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    19261 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    19321 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    19381 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    19441 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    19501 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    19561 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    19621 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaatgcgga tcaggataca
    19681 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    19741 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    19801 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    19861 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    19921 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    19981 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    20041 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    20101 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    20161 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    20221 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    20281 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    20341 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    20401 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    20461 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    20521 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    20581 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    20641 tatgctagag gaaacaaaaa tacacgaaaa agaaaaagta actccgacga atacgaatac
    20701 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    20761 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    20821 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    20881 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    20941 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    21001 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    21061 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    21121 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    21181 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    21241 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    21301 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    21361 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    21421 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    21481 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    21541 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    21601 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    21661 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    21721 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    21781 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    21841 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    21901 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    21961 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    22021 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    22081 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    22141 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    22201 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    22261 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    22321 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    22381 aaaatggtca aaacttttcg gaaaaagaag tgttcttcaa aaaaggcgat gatttactga
    22441 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    22501 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    22561 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    22621 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    22681 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    22741 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    22801 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    22861 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    22921 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    22981 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    23041 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    23101 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    23161 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    23221 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    23281 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    23341 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    23401 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    23461 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    23521 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    23581 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    23641 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    23701 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    23761 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    23821 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    23881 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    23941 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    24001 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    24061 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    24121 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    24181 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    24241 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    24301 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    24361 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    24421 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    24481 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    24541 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    24601 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    24661 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    24721 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    24781 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    24841 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    24901 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    24961 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    25021 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    25081 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    25141 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    25201 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    25261 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    25321 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    25381 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    25441 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    25501 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    25561 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    25621 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    25681 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    25741 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    25801 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    25861 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    25921 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    25981 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    26041 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    26101 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    26161 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    26221 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    26281 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    26341 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    26401 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    26461 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    26521 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    26581 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    26641 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    26701 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    26761 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    26821 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    26881 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    26941 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    27001 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    27061 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    27121 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    27181 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    27241 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    27301 gaaatacttg cacatatcga tcgaatccga gataatacct acgcttatct gattgtagat
    27361 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    27421 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    27481 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    27541 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    27601 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    27661 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    27721 taattccttt tattcaaaaa acataccaaa aatattccaa catcatcttg gactttctac
    27781 atgttactaa tgagcgtgta aaagaacgag atggtgtttt tatttctatc aatgaagctc
    27841 tttttctttg caatcctcac ttcaatcctc tataatgtcg catatagttt agtaaatgag
    27901 taaactacta atttacttat ttactaaaaa taaaaggatg aggagttaaa ataatggaaa
    27961 taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga agactatgat aaagttgatg
    28021 gaagaaatgc attaaatacg gatgcaaaag ggctttcact gggccttgct cagtggaatg
    28081 atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca tactggagaa aaatggtcac
    28141 gacagtcgga agaattacca atcacacgta ttttagattt agcgatctta gctgcacaag
    28201 gaagtttata cttccaagat gcttatcgtt atgaaaaatt ttatgatcca gaaaatccag
    28261 tcgtagacat cattggatta caagggaacc gaatgacggt agaagtagat accaaaaatc
    28321 caaagatcga tgaagacatc attttgtatt acgatacact acaaaaagac ggagaactga
    28381 ttggggaacg attccgtatt ttaaaacgtc ttttagatga gctaggatat taatagacag
    28441 agatgatcaa aaggggaaga gaagcatgga gaatttctca ctaacagaaa accgaacctt
    28501 ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa agccatcgga caagtgcaga
    28561 agaatggcgg atcgcctccg aaatcaaagg gaattggcaa gatcctgaaa tgaaaatcag
    28621 taacgtactt ttggaaggag atgccgggtc ggggaagaca caattagcca aagcactatc
    28681 tgctgatctg caacttccct atacaaaagt gacctgtttt gcagatatgg acaaatcaga
    28741 tgttttcgga gccttattac ctgtagtaga ttctaataag gaacaagacc aagaattact
    28801 agaagctatt taccaaacag atacacttga agctgtactg acactgatcc agaaccatta
    28861 cggcattgat cctttttctg ctaaagaaaa attagcagag ctagttcaaa ggatcgaagc
    28921 tgattatcca gatgcaatcc attacagcta ttacccttct gaaatcgttc gagcgattga
    28981 aaaaggatat ttattagaga ttcaagagcc gactgtcatc cgagatgcct ctgttcttgt
    29041 ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat ttaccgacag gaatcattcg
    29101 tcgccatcca gattgcgtca ttgtgatcac tacaaatcga aattaccaag gaaatcgccc
    29161 attaaatgaa tctttaagag atcggatgca acatgcggaa aaaatggatc ttcctgcctt
    29221 agaagtcatg gcagaacgtg cgatggcgaa aactatgatc caaaaacaag aacttctctt
    29281 aaagatggca gagattatcc gtttgttgga tgaaactgca aaagctaacg ccatcaaagg
    29341 tgtagcagga atgcgttctt atttttattg ggcaaatacc tataagcaag gacaagattt
    29401 actccagtcg atttatccaa aagttttgta taaattatcc actgattcag atgaattggc
    29461 aattcttgaa caagctttgt ctgacagtgg attgctactg gaacttcagg acatattgag
    29521 aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa ggaagaacca ttagcgccga
    29581 agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa attctgcgct tagatgaaaa
    29641 gaaatcgttg tctgctgaag aaccaaatga gggagaagag aaacaagaag aaacaataga
    29701 aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt agcgatatcg aaggaaaaaa
    29761 tcaaaaagaa caacaaacag gagatgattc tcaagaaatg tctgcttctg acatggaagc
    29821 acatgataag gaaatcaaga agcaactgaa taaagaagcg cgacaaatca tgaaggaaac
    29881 gattcatgaa aaagaaggtc tcatcgttca ccgaccagct ttccaaggaa aaacgccgga
    29941 agcacgtgag ctacacatgc aagtaatgcc aatagtagaa tcacttagcc ggcaaatttt
    30001 agagttactg gataatgaac aaacagagac gtaccaaaaa ggaaaatacg aaggtcagcg
    30061 gttcaatgca agcaaagtag catatggtga tttgagaaat tttgacaaaa agaacccgcc
    30121 acatgagcaa ccttctttag cggtagctgt acggatcgat gaatctgggt caatgatacg
    30181 ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc attgccgaat tttctaagaa
    30241 agtcgatatc ccattgttga tttacggaga tacagcagac cgttcgtcaa gagaaaagac
    30301 ttctttgttt tcttataaag aatttgaaga cggatttcaa tggttaaatg aaaaattagt
    30361 cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca cttcgtttag cagcagaaaa
    30421 attaaacaga caatccgcaa caaccaaact gttgcttaat attagtgacg gtcagccaaa
    30481 agcgttgccg gattatacag gcaaaaaagc aaaagaagat atccagtctg ttttgaaaga
    30541 atatgaacgt caaggaatcc tatttgtctc cgcagctatt ggccaagaca aagaagaaat
    30601 caagtcgatc tacggtgaaa gtcgttttgt agatattact gacttgaatg aatttcccaa
    30661 gcaaatgatt caactgattg cccgctatct atgataaaat aaagctcaac gaacgcactt
    30721 agcagattcg ttgagctttt cgtttctagt taccggtaac aaggatactt ctaaaataga
    30781 atcggttata atcaagaggg accgttaaag gagtgaaacg atatgataca agaacaaaaa
    30841 tggtggaaag aagcagtagg ctatcaaatc tatcctgcta gtttcaaaga tagcaatggc
    30901 gatggtcaag gagacatcaa tgggatccgt gaaaaactta attatttgaa agatcttggt
    30961 atcggcttta tctggatcaa tccgatctat caatctccat ttgtcgataa tggttatgac
    31021 gtaagtgact atcaagatat agaagagaga ttcggaacga tggaagattt tgatttattg
    31081 ctaaaagaag cacatgaatt agggatcaaa gtgattatgg atcttgtcat caaccatagt
    31141 tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta aagaaagccc gtatcgcgat
    31201 tattacattt gggtcgacgg ggtagatggc aaagaaccga ataattggac atcgattttc
    31261 ggcggttctg cttgggagta ttctcatgaa actggacagt attatctgca tgtttttgca
    31321 aaagaacaac cagacttaaa ttgggaaagc gagaaattga aagaagaact attcaatatg
    31381 atccgatggt ggttggataa agggatagat ggatttcgtt tagatgcgat ttctcatgta
    31441 aaaaaagatg aatattcagt aaaagccaca gagaaccctt tctcaccctt tcaaaatgtc
    31501 tcaggaatcg aagaacattt gaccgatttg aaacatgttt ttgaagagta cgatatcatg
    31561 actgtcggag aagcaagtgg tgtcactgca gaagaaggac cacaatgggt agggaaagac
    31621 ggttatttcg atatgatatt cgaatttgat catatccata tctggcagca agaaaaagaa
    31681 gggcaattag atgtccttaa attgaaactt gcattaagcg cttggcaaac ttcattggat
    31741 ggtatcggat ggaacgcttt atatatggaa aaccatgatg ttcctcgtgc cgtttcagtg
    31801 tttggagata ctcgtcccga tttttgggct atgtctgcaa aagcaatcgc tatgatgtat
    31861 ttctttttgc aaggtacgcc atttatttat caaggacagg aaatcggaat gacgaatatg
    31921 ccctttgaat cgatcgatca agttgatgca gtagattcga aaagacttta taaaaggctg
    31981 cttgcggaag gtaagacaag agaagaagca ttggatatca ttcgtgaaac aacgagagac
    32041 aacagcagga cacccatgca atggacttcg gaacaatatg ctggtttttc tacacatgaa
    32101 ccttggctga tcactaatcc aaataccaaa acgatcaatg tagaacaaca agaatatgaa
    32161 ccagagtcgg tcctgcaatt ctataagaac atgatccgca ttagacaaac caataaaggt
    32221 ctgatatacg gttcttataa agaatatctt catgaacatc cacaacttta tgtctatgaa
    32281 agatatttag aagatgaaca atatcttatt atggtaaact taacagaatc acttgctgac
    32341 tatgaacttc caaaagaagc agatcagtcc tggacattac tcttgtcaaa ttcctctagc
    32401 ggagaatttg aagctaaagg catactagca ccttatgaag ctcggttata taaaacaaac
    32461 aagtaaaact tcctcgttaa agttcattag ttggactttg cgaggaagtt tttcagattg
    32521 ctctgtcaat tactcatttc aataattcgc tctaggacca ccgatatatt taggacgaga
    32581 gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa cgaggtctga caggtacttg
    32641 gacatgtttg atatgcatac cgattgatgt gtcaccgata tcgataccgg cttgtccaac
    32701 tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa gcagcaacag aacaagcacc
    32761 tccagcatgt agtgcaggaa caacagagac aatttcgaaa ttttttgctt ctgccactgt
    32821 tcgttctact actaatgctc ggttcaaatg ttcacaacct tggacagcta gcgcaatttc
    32881 tttaggatcc aacaactctt ttaacgtacg gatgatccat tgaccaactt ccgcactgga
    32941 attttttcca atatgtcctc caacgacttc gcttgtgctg catcctaaaa caaaaagatc
    33001 accttttttc aagtgtgcct cagccaatat gtcattaact atttcagtca attggtcttt
    33061 taattctttt tctgacaact tcattttcta aaaccttctt tttcaattaa tattgcttcc
    33121 gtgcttgaat acgttgaaga cttgcagtta taggaaaggc aagtcccatt ccaatcagat
    33181 tttggaagat gtttcctggt atagaagcca ctccagccgg ccaatcatat aaaatccaac
    33241 cggcgatacc atagccgatg atcataacta agcttcctaa taaaagccct aatccccaca
    33301 tacgagtaga agatttgctg aaagaaccag caacatatcc ttgtaacccg tggatcacta
    33361 atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc gatcaataga ccactggaag
    33421 caccaacaac taatccaccc gttggaccat aaagaaaagc agcggtatag atcccggcct
    33481 cgcataatgt cacaaatcca ttcgtagccg gtacagggat caaaattgtt aatgaaaggg
    33541 caactgtcaa cgcaatcagc atagcataga tcgttaattg cttagttttc atttctcatt
    33601 cctccttttt gaataccgcc agaccagaca cttccggtat gatcgttcat cagcacgcca
    33661 ttagaaatac aatgggcaac aaatttttta ctttctgcta ctgctttggt aaaatctgcc
    33721 cctaacacaa gattcgctgt tattgcagaa gaaaaggcac aaccggcccc gtttactgtt
    33781 gtagtgtcga tttttttatg agaaaatacc tcataatgat caattgaata gagaagatcc
    33841 acagcttcat ctccagccat ccttgtgccg cctttgatca caatgttttt tgttcctgaa
    33901 gcagtgagtt ctttagcgag atggatcatt tctgttaacg aagagggaag agcttgacca
    33961 gaaagtaaag cagcttctgc caaattaggc gtaatcatcg taacaagtgg aaaaagttct
    34021 tcgatgagct gcgaaatata caattgattg atcacagctt cagtttcttt gaatgccaaa
    34081 acagggtcta aaatgattgg gatattttgt tttgttttta tgaagtctcg aacaaccatg
    34141 attgcttcac ttgaattcaa taacccaatt ttgataccat ctaatgaagt cattttatca
    34201 atcgaaacta actgtcgttt aagcaattcc aaagatatag attccagcat aaatcgctca
    34261 ttaatatcta gtgcccctat agaagtcaag gcgcttagtc cgaatagacc aaaattctca
    34321 aaagttttta aatctgtttg gattcctcca ccagcaaatg gatcagaacc gccaattgtc
    34381 aagattcttt tcataacatt ttcctccatg aataagagta taacgaaatt ttcaaaaaag
    34441 aaaaacagcc aattggcata tttttatctc atccaattta aaaaaacaaa aagaaaagaa
    34501 agaccaaaat ggatctttct ttccttaaat agttaggact tttgtgattt taattctttc
    34561 tcgatttgaa gccgttgcac tttcattgta gctgttcgcg ggattgcatc aaaatcacgg
    34621 atgataggga cgttcaagcg aggaagttca tggacttgct cccaccatcg attccaatcc
    34681 atttctttat caggagctaa agcaataatc ggttgtggag aatgattttt atcccttaca
    34741 atcactactt ctgccaaaaa atctaacgaa tctaataaaa agtcttcaat agctaatgta
    34801 ctattgatcg tttcgatcaa atccacttgc cgatctttca aaaataggtg gccatgctta
    34861 tccataaaac cgtaatctcc gctatcccac caatcgccat aaacattttc ctggaaccgt
    34921 gcatcttcct tgtaataagt caaagcccgt cctttagaca gtaattgtat atgaccatct
    34981 gtgttttcag gtaggacatt cccaactgaa tctgtaatcc gagcgcttgt catgtcttcc
    35041 agtccaacgc ccatatctct tgcatcactt gttttcaaac tctctagtgt atgagcctta
    35101 agaatcattg gtccgcattc actttggcca tatacttgaa gaaataccgc atcctgtgcc
    35161 atcgacgttc gcaaaaatgt cttcattgtc tggttgttta tagcatcaaa agtggagtga
    35221 tagtatcgga tacccgaaaa agcttctgga tgctccttag cagtaaaacg ccactgaaca
    35281 aagttattcg gatgggtctc tagtgcaatt ggccggtgag cttccaacat atgaacaact
    35341 tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc ccatggacat caaagaagag
    35401 ataccaatat tgaatctgga atgcactgga gagatgtgaa aagcgattaa tttcttttcc
    35461 gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc atcccataga atgagcagaa
    35521 tgacaaataa gtttagggat accagttgta ccagaagtat gggtcatata agaaatttca
    35581 tttttgtcta aaaatgattg agaaacagac aaagcaggtt gttccattaa ataaagaata
    35641 gga
//
LOCUS       DAYYAR010000015        34449 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000015 DAYYAR010000000
VERSION     DAYYAR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.015"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACHAT0_000617"
     CDS             352..1041
                     /locus_tag="ACHAT0_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8450584.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACHAT0_000618"
     CDS             1028..2236
                     /locus_tag="ACHAT0_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8450585.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACHAT0_000619"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACHAT0_000619"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8450586.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHAT0_000620"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHAT0_000620"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8450587.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACHAT0_000621"
     CDS             complement(6339..7034)
                     /locus_tag="ACHAT0_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8450588.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHAT0_000622"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHAT0_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8450589.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACHAT0_000623"
     CDS             complement(8233..8424)
                     /locus_tag="ACHAT0_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8450590.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACHAT0_000624"
     CDS             complement(8445..9335)
                     /locus_tag="ACHAT0_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8450591.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACHAT0_000625"
     CDS             complement(9322..10089)
                     /locus_tag="ACHAT0_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8450592.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHAT0_000626"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHAT0_000626"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8450593.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHAT0_000627"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHAT0_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8450594.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHAT0_000628"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHAT0_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8450595.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACHAT0_000629"
     CDS             14660..15043
                     /locus_tag="ACHAT0_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450596.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHAT0_000630"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHAT0_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8450597.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACHAT0_000631"
     CDS             complement(15987..16805)
                     /locus_tag="ACHAT0_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8450598.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHAT0_000632"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHAT0_000632"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8450599.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHAT0_000633"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHAT0_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8450600.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17934..19268
                     /gene="dnaA"
                     /locus_tag="ACHAT0_000634"
     CDS             17934..19268
                     /gene="dnaA"
                     /locus_tag="ACHAT0_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8450601.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19466..20596
                     /gene="dnaN"
                     /locus_tag="ACHAT0_000635"
     CDS             19466..20596
                     /gene="dnaN"
                     /locus_tag="ACHAT0_000635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8450602.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20825..21067
                     /gene="yaaA"
                     /locus_tag="ACHAT0_000636"
     CDS             20825..21067
                     /gene="yaaA"
                     /locus_tag="ACHAT0_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8450603.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21054..22178
                     /gene="recF"
                     /locus_tag="ACHAT0_000637"
     CDS             21054..22178
                     /gene="recF"
                     /locus_tag="ACHAT0_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8450604.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22175..24121
                     /gene="gyrB"
                     /locus_tag="ACHAT0_000638"
     CDS             22175..24121
                     /gene="gyrB"
                     /locus_tag="ACHAT0_000638"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8450605.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24141..26612
                     /gene="gyrA"
                     /locus_tag="ACHAT0_000639"
     CDS             24141..26612
                     /gene="gyrA"
                     /locus_tag="ACHAT0_000639"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8450606.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26848..27147
                     /gene="rpsF"
                     /locus_tag="ACHAT0_000640"
     CDS             26848..27147
                     /gene="rpsF"
                     /locus_tag="ACHAT0_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8450607.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27197..27709
                     /gene="ssb"
                     /locus_tag="ACHAT0_000641"
     CDS             27197..27709
                     /gene="ssb"
                     /locus_tag="ACHAT0_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8450608.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27735..27971
                     /gene="rpsR"
                     /locus_tag="ACHAT0_000642"
     CDS             27735..27971
                     /gene="rpsR"
                     /locus_tag="ACHAT0_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8450609.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28114..30087
                     /locus_tag="ACHAT0_000643"
     CDS             28114..30087
                     /locus_tag="ACHAT0_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8450610.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30093..30545
                     /gene="rplI"
                     /locus_tag="ACHAT0_000644"
     CDS             30093..30545
                     /gene="rplI"
                     /locus_tag="ACHAT0_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8450611.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30819..32186
                     /gene="dnaB"
                     /locus_tag="ACHAT0_000645"
     CDS             30819..32186
                     /gene="dnaB"
                     /locus_tag="ACHAT0_000645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8450612.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32966..34258
                     /locus_tag="ACHAT0_000646"
     CDS             32966..34258
                     /locus_tag="ACHAT0_000646"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8450613.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10504 a         6696 c         6824 g        10425 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgcggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggagtcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa atggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggtgattt cctgtgtatt ccgtctccta actattggta tatctatatt
    17701 ttttatccac agatgcaaga ggaaatctat tgatttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt tttgacaact gtgtttttct cttttttctt tttgtcgatt
    17821 gtgtggataa ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa ctattgaaaa
    17881 aaatgctaaa ttagtactag ttattcactt tttaatgaag gaggaggcct tatatggtat
    17941 ccctcgatgc tttatggaat gaattaaaag caacatacca aaaggattta tcgcctgcta
    18001 gctataatac atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac cagctcgttg
    18061 ttgaggttcc tagcaagatt cataaagaat attgggaaaa aaacctggcg acaaaaatcg
    18121 ttgaagtcgg ttatatgtta tcaggaaatg agattatccc tcgctttatt actggcgaag
    18181 aagcagagca agaagaagtt atagaagaaa aaaatccaaa agttgtggca cctagtccgc
    18241 taaaaaaagc catgctgaac cctaaatata cctttgatac gtttgtcatt ggtaaaggaa
    18301 accagatggc tcatgcagca gcgctcgttg tagcagaaga tccaggttct atttataacc
    18361 cgctcttctt ttatggaggc gtgggacttg gtaagactca cttgatgcat gcaattggtc
    18421 atcaaatgct gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt gaaacttttg
    18481 ctaatgattt catcaattcg atccaaaaca aaacagcaga agagttccgt caagaatatc
    18541 gaaatgttga tttactgcta gtggatgaca ttcaattttt tgcagaaaaa gaagctacgc
    18601 aagaagagtt tttccatact ttcaatgcct tgtacaatga agggaaacaa atcgtactaa
    18661 ccagtgatcg gttgccaaac gagattccga aactgcaaga acgcctcgtt tctcgctttg
    18721 catgggggct gtctgttgat attacaccgc cggatcttga aacaagaaca gcgattttac
    18781 gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga cacactaagc tacatcgcag
    18841 gacaaattga ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg caagcttttg
    18901 ctgcgatgaa tagtgaagat atttcgacta gtctagctgc agacgcactg aaaacgttaa
    18961 aatcaggaaa aggacatcct caattgtcaa tcctgcagat ccaagaagaa gtggcaaagt
    19021 attatcacat ccaattgaaa gatatcaaag gaaagaaacg cgtcaaatct attgttgttc
    19081 ctagacaaat cgctatgtat ttggcacgtg agctcacaga taattcttta ccgaaaatcg
    19141 gagctgaatt cggtggcaag gatcatacaa cggttatcca tgctcatgaa aaaatccagc
    19201 agctgatgga ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa aactttttac
    19261 taaattaatt tgtcgattct atcaactgaa aatagcatgt ggataaaaga aaaaaacgtt
    19321 caaaagttat gcacactttt tccacagctg ttttttcgtt tctctcttac gttttttgag
    19381 ttttccacag aatcaacatg ccctattact tttattattc tttttaacta ataataaata
    19441 aacaaacatt caaaggagat acatcatgaa agttacttta aaccgagcta gctttatgca
    19501 ggaattgcaa actgttcaac gagctatttc aagcaaaacc acgatcccta ttttgacagg
    19561 tgtaaaaatc acactgacac aagaaggttt gactttgacg gggagcaacg ctgatatatc
    19621 aattgaaact tttttgtctg ttgaaaacga aaaagcaaat atgcaaatcg aatctactgg
    19681 ttccattgtt ttacaagcac gtttctttag cgaaatcatt cggagacttc ctgaagaaac
    19741 atttacttta gaagttttag aaaataaaca agtagcgatt acttctggaa aagcgaattt
    19801 tatcgtaaat ggattagatg cagataacta tcctcatctt cctgttgtcg aaagccataa
    19861 ccagatgaaa ttacctgtac acgtattgac taaactaatc aacgaaacag tttttgctgt
    19921 ctcacaacat gagagtcgtc caatcttgac aggtgtgcat ttcattttat ctgataattc
    19981 tttattggct gtagctactg attctcaccg tctaagtcaa cgcgtgattc cagtagaaca
    20041 agcggctgat cattttgata ttgttattcc tggaaaaagt ttgatcgaat tatctcgctc
    20101 attaacaaat gaagaagaaa tcgtcgaaat cagcattatg gaaaaccaag tgttgttcaa
    20161 aacagaaacg atgtatttct attcccgttt gctagaagga aactatcctg ataccaatcg
    20221 tttgattcct tcaagtttta atacagaggt tgaattttct gttcctagct ttttagcggc
    20281 gatcgaacgg gcttctttac tttctcatga aggccgtaac aacatcgttc gtttatctat
    20341 tcgtccagat gcggttgttt tatacggaaa ttcgccagaa atcgggaaag tagaagaaag
    20401 cttgagttat acagcaagca gtggcgatcc attagatatt tctttcaatc cggattatat
    20461 gaaagcagcg ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta tctctgctat
    20521 tcgtccattt acattggaac caacggaaga tggcgttcaa tttattcagc tgattactcc
    20581 tgtacgtaca aattaacaat gattttcatt gttcttgaaa atttaattga aaagaaatag
    20641 aaaaaataaa attttaaaca gaaggcccta aaatctcttt taaggctttc tgtttatttt
    20701 tgtttaaatc acctttttta gattaaatcg tcttatatgg aaaaatatat ggataaattt
    20761 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
    20821 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
    20881 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
    20941 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
    21001 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
    21061 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
    21121 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
    21181 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
    21241 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
    21301 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacaaaaaa
    21361 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
    21421 tagtaaaagg cagcccgcag atcaggcgga agtttttaga tatggaatta ggccagatca
    21481 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
    21541 atttaaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
    21601 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
    21661 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
    21721 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
    21781 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
    21841 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
    21901 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
    21961 ttaaagaaga gacgggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
    22021 atgatagtcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
    22081 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
    22141 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
    22201 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
    22261 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
    22321 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
    22381 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
    22441 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
    22501 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
    22561 tgttgttaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
    22621 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
    22681 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
    22741 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
    22801 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
    22861 cggcgggatt aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
    22921 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
    22981 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggcgg
    23041 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
    23101 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
    23161 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
    23221 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
    23281 attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat tactagcttc
    23341 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
    23401 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
    23461 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
    23521 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
    23581 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
    23641 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
    23701 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
    23761 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
    23821 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
    23881 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
    23941 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
    24001 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
    24061 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
    24121 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
    24181 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
    24241 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
    24301 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
    24361 tcatgggtaa ataccatcct catggagact atgcgatcta tgagtcaatg gtacggatgg
    24421 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
    24481 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
    24541 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
    24601 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
    24661 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
    24721 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
    24781 ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc cgaagagcgt
    24841 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
    24901 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
    24961 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
    25021 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
    25081 ctgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
    25141 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt cttgaaaact
    25201 atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa gaaaaagcag
    25261 aagcaagggc acacatttta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
    25321 ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg atcgaacggt
    25381 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
    25441 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
    25501 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
    25561 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
    25621 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
    25681 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
    25741 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
    25801 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
    25861 ttccagaata cgggcgcaca gccaaaggaa taccaattat caatttacta ggtatcgatt
    25921 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
    25981 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
    26041 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
    26101 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
    26161 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggttaga ggaatacgcc
    26221 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaagatcaa gaagtattag
    26281 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
    26341 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
    26401 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
    26461 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
    26521 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
    26581 aaacagttga agaacaagga tcagaagaat agccatcaag actaattgtg taagctagca
    26641 agaatgaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
    26701 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatc
    26761 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
    26821 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
    26881 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
    26941 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
    27001 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
    27061 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
    27121 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
    27181 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
    27241 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
    27301 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
    27361 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
    27421 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
    27481 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
    27541 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
    27601 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
    27661 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
    27721 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
    27781 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
    27841 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
    27901 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
    27961 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
    28021 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
    28081 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
    28141 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
    28201 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
    28261 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
    28321 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
    28381 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
    28441 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
    28501 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
    28561 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
    28621 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattggat
    28681 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
    28741 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
    28801 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
    28861 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
    28921 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
    28981 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
    29041 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
    29101 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
    29161 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
    29221 atcgttatca acgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
    29281 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
    29341 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
    29401 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
    29461 ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc agagctagtt
    29521 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
    29581 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
    29641 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
    29701 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
    29761 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
    29821 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
    29881 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
    29941 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
    30001 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
    30061 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
    30121 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
    30181 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
    30241 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
    30301 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
    30361 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
    30421 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
    30481 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
    30541 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
    30601 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
    30661 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
    30721 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    30781 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    30841 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    30901 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    30961 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    31021 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    31081 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    31141 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    31201 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    31261 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    31321 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    31381 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    31441 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    31501 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    31561 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    31621 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    31681 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    31741 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    31801 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    31861 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    31921 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    31981 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    32041 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    32101 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    32161 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    32221 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    32281 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    32341 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    32401 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    32461 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    32521 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    32581 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    32641 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    32701 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga
    32761 agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt
    32821 ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa
    32881 taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt
    32941 aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg
    33001 cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg
    33061 gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact
    33121 gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt
    33181 tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga
    33241 aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt
    33301 ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat
    33361 cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga
    33421 taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt
    33481 aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata
    33541 cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga
    33601 tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg
    33661 aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg
    33721 tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt
    33781 cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt
    33841 aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt
    33901 agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga
    33961 tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact
    34021 aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga
    34081 attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa
    34141 cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc
    34201 tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg
    34261 ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata
    34321 cgataataat aacttgaaga ggtagtgaaa gagacgttta gccttattgc cgataggctg
    34381 tctcttgaac actgtttatt cagaataagg gactgtgaca aaaaccgtgt cacagtccct
    34441 ttttccgaa
//
LOCUS       DAYYAR010000016        34412 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000016 DAYYAR010000000
VERSION     DAYYAR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.016"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(388..723)
                     /locus_tag="ACHAT0_000647"
     CDS             complement(388..723)
                     /locus_tag="ACHAT0_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8450614.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(710..994)
                     /locus_tag="ACHAT0_000648"
     CDS             complement(710..994)
                     /locus_tag="ACHAT0_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8450615.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1050..2573)
                     /locus_tag="ACHAT0_000649"
     CDS             complement(1050..2573)
                     /locus_tag="ACHAT0_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8450616.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(2566..3741)
                     /locus_tag="ACHAT0_000650"
     CDS             complement(2566..3741)
                     /locus_tag="ACHAT0_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8450617.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(3745..3930)
                     /locus_tag="ACHAT0_000651"
     CDS             complement(3745..3930)
                     /locus_tag="ACHAT0_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450618.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(3896..5590)
                     /locus_tag="ACHAT0_000652"
     CDS             complement(3896..5590)
                     /locus_tag="ACHAT0_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8450619.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(5587..6060)
                     /locus_tag="ACHAT0_000653"
     CDS             complement(5587..6060)
                     /locus_tag="ACHAT0_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8450620.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6129..6284)
                     /locus_tag="ACHAT0_000654"
     CDS             complement(6129..6284)
                     /locus_tag="ACHAT0_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8450621.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(6418..6798)
                     /locus_tag="ACHAT0_000655"
     CDS             complement(6418..6798)
                     /locus_tag="ACHAT0_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8450622.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(6802..7017)
                     /locus_tag="ACHAT0_000656"
     CDS             complement(6802..7017)
                     /locus_tag="ACHAT0_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450623.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7020..7427)
                     /locus_tag="ACHAT0_000657"
     CDS             complement(7020..7427)
                     /locus_tag="ACHAT0_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8450624.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(7693..9168)
                     /locus_tag="ACHAT0_000658"
     CDS             complement(7693..9168)
                     /locus_tag="ACHAT0_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8450625.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9158..10006)
                     /locus_tag="ACHAT0_000659"
     CDS             complement(9158..10006)
                     /locus_tag="ACHAT0_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8450626.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10043..10405)
                     /locus_tag="ACHAT0_000660"
     CDS             complement(10043..10405)
                     /locus_tag="ACHAT0_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450627.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(10406..10564)
                     /locus_tag="ACHAT0_000661"
     CDS             complement(10406..10564)
                     /locus_tag="ACHAT0_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450628.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(10705..11329)
                     /locus_tag="ACHAT0_000662"
                     /pseudo
     CDS             complement(10705..11329)
                     /locus_tag="ACHAT0_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(11326..11637)
                     /locus_tag="ACHAT0_000663"
     CDS             complement(11326..11637)
                     /locus_tag="ACHAT0_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450629.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(11681..11974)
                     /locus_tag="ACHAT0_000664"
     CDS             complement(11681..11974)
                     /locus_tag="ACHAT0_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450630.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12168..12815
                     /locus_tag="ACHAT0_000665"
     CDS             12168..12815
                     /locus_tag="ACHAT0_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8450631.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            12876..14021
                     /locus_tag="ACHAT0_000666"
     CDS             12876..14021
                     /locus_tag="ACHAT0_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8450632.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14102..14407)
                     /locus_tag="ACHAT0_000667"
     CDS             complement(14102..14407)
                     /locus_tag="ACHAT0_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8450633.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(14432..14863)
                     /gene="ruvX"
                     /locus_tag="ACHAT0_000668"
     CDS             complement(14432..14863)
                     /gene="ruvX"
                     /locus_tag="ACHAT0_000668"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8450634.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(14860..15126)
                     /locus_tag="ACHAT0_000669"
     CDS             complement(14860..15126)
                     /locus_tag="ACHAT0_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8450635.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(15381..16106)
                     /locus_tag="ACHAT0_000670"
     CDS             complement(15381..16106)
                     /locus_tag="ACHAT0_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8450636.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(16467..16537)
                     /locus_tag="ACHAT0_000671"
     tRNA            complement(16467..16537)
                     /locus_tag="ACHAT0_000671"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16503..16505),aa:Gly,seq:ccc)
     gene            16654..17406
                     /locus_tag="ACHAT0_000672"
     CDS             16654..17406
                     /locus_tag="ACHAT0_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8450637.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNNLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            17422..17901
                     /locus_tag="ACHAT0_000673"
     CDS             17422..17901
                     /locus_tag="ACHAT0_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8450638.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18152..19732)
                     /locus_tag="ACHAT0_000674"
     CDS             complement(18152..19732)
                     /locus_tag="ACHAT0_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8450639.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19725..20468)
                     /locus_tag="ACHAT0_000675"
     CDS             complement(19725..20468)
                     /locus_tag="ACHAT0_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450640.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(20644..21420)
                     /locus_tag="ACHAT0_000676"
     CDS             complement(20644..21420)
                     /locus_tag="ACHAT0_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8450641.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(21668..22504)
                     /locus_tag="ACHAT0_000677"
     CDS             complement(21668..22504)
                     /locus_tag="ACHAT0_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8450642.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(22515..23444)
                     /locus_tag="ACHAT0_000678"
     CDS             complement(22515..23444)
                     /locus_tag="ACHAT0_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8450643.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            23636..23989
                     /locus_tag="ACHAT0_000679"
     CDS             23636..23989
                     /locus_tag="ACHAT0_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8450644.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24063..24719)
                     /locus_tag="ACHAT0_000680"
     CDS             complement(24063..24719)
                     /locus_tag="ACHAT0_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8450645.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            24945..26879
                     /locus_tag="ACHAT0_000681"
     CDS             24945..26879
                     /locus_tag="ACHAT0_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8450646.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(26965..28188)
                     /locus_tag="ACHAT0_000682"
     CDS             complement(26965..28188)
                     /locus_tag="ACHAT0_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8450647.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(28182..30053)
                     /locus_tag="ACHAT0_000683"
     CDS             complement(28182..30053)
                     /locus_tag="ACHAT0_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8450648.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(30074..31192)
                     /locus_tag="ACHAT0_000684"
     CDS             complement(30074..31192)
                     /locus_tag="ACHAT0_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8450649.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(31275..32108)
                     /locus_tag="ACHAT0_000685"
     CDS             complement(31275..32108)
                     /locus_tag="ACHAT0_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8450650.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(32101..33018)
                     /locus_tag="ACHAT0_000686"
     CDS             complement(32101..33018)
                     /locus_tag="ACHAT0_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8450651.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(33041..33220)
                     /locus_tag="ACHAT0_000687"
     CDS             complement(33041..33220)
                     /locus_tag="ACHAT0_000687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450652.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(33245..34369)
                     /locus_tag="ACHAT0_000688"
     CDS             complement(33245..34369)
                     /locus_tag="ACHAT0_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8450653.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        10306 a         6957 c         5645 g        11504 t
ORIGIN      
        1 atcgagcatc acaaaatctg tggtattaat aaactcattt tctaaatgta gataatactt
       61 ccaataaaaa tttgtaaact ctgctttgtc cattaaatca cctcttttga attataacaa
      121 aaaaaacacg tccacaacag acgtgttctt tgatttctac acaatcagct tgccaaaacc
      181 atccaatcag tcctagcatg cctgtattaa aattgaacgt tcatcatcta tcatctttct
      241 tagtccagca tgtttgcaac cacacctaca attttcagtc caacaataga tttcgactag
      301 ctaagtcttg caaaattcag gcagctaacc cttcatttac taccttaatt atactaaata
      361 cccacaacgt ttacaagtgt aatcattcta agaaatctgt ttagctatga tcgttgtata
      421 atctttaaac gccgttcctt cattgattga aataatttta taactcttgt ttttccaagc
      481 tactttcata tcgtttgtta gttcataatc ttgatcgtat cgaataataa acgtcaacgt
      541 tccttcaaga atggtaccta cactagcaag cacgtctttt agcatttgtg aacgcacatt
      601 agcccaacat tcaaatacag tttcttcact atccaccatc tgcccatctt catcttttac
      661 ccgttttgtc tttttaaact tgactctctc agtcaattca tttacattct taatgattgg
      721 cataataagc acctctcatt tgttgtacta atgcttgtac cgttactggt atccgttcgc
      781 tgctggcttc ttgacggttc aaataccaat gttgagtaag taatgacacc gccaaatcaa
      841 attgctgata attatccata tctttatccg ttgcgctact atcaatagca ctttttatat
      901 attcgcctgc tgtatcaatc aattgttgaa taaagtcatc atcaatagtg tgatcgatcc
      961 gcatactttt ttttatgcta tccaaaatta ccattttatc tctcctaatg aaaaaaaggc
     1021 agtgggataa accgccgccc aatatttttc tatacttttg gtgttacaac tggcgtaaac
     1081 tcaatataca ctgaagcatt ttgatcaatt ttcttataat cgttgcgaac gatcactgca
     1141 agtccttgtg agtagtaatc gaatttttcc cattgagtcg ttacttgatt tctacgagca
     1201 acaaagatag attgtgcaat atcccccata atcataggga atgttccagc tttaggattg
     1261 gcaaacaatg tatctgcaat caatactact ggcataccga ataatgattt accactaggt
     1321 gctgtcacat cgggttgtaa aatgtaacgt ccatctgaat ctttcaatgt atctaggtgg
     1381 taatatccgc tttggtttag aattaccatt ttatttaatg ctgggtctaa tgccacattg
     1441 tatagttgtt tcaaatcgtc caacgtagca gccgttttct tagtgaatgt ttttaataaa
     1501 tccatgatat gcttattgtc agtgttttct accaatttcg ctaactgatc tttgacctct
     1561 tgtacaatat tcactgctga atcttccaca acctcatttg ataaggcaat cttaccagca
     1621 cgagtttcta ctttataatc aactgaagta aacatttcag cgtcaatatc accaatttca
     1681 gcaagttcag cttttgttgc taacactgct tgttggttag ttgctactgg atatttacct
     1741 tgtccatttg atactgtttt tactgttgca tattgagcta agttataatt tgaacgtttc
     1801 aaatcaaaga cttcaccgat tacttcttcg ggaacaactg ccgctgcatt tacagtagtg
     1861 actccatcac gcacttcgcc ttgtgaacgg atatattctt catacccacg caattctgtt
     1921 ttttcgttat cgattaaagt tttttccatg ttattactct ccttttgtgc tttagaagtg
     1981 gattctaagc tattgtttgt ttgttttgct tggttgttac tcataaacga ttcgtaagaa
     2041 cgcttattta cttggacatt tgatgaatca taagcgggaa cggtcacgac actaatttca
     2101 ttaagtgctt tgattttatc tattgatcgt tcaattgtgc catcttcttt tttgtcgaat
     2161 gaatctaatc caagcacgaa cccaaaggac atagaatcaa caaccccttt tgagatattc
     2221 tcatacacat catttgaata ggtcgtgtcg gttagctctg cttcaaaatg taatcctgta
     2281 tcatcgacat tcaatttcaa cgtacctgct ttgacactag ctagtggctt tgaataatcg
     2341 tgtccatata ataagaacac atttgataaa tccacctctt ttaatgcttc gggtgtaatc
     2401 acttctacaa agccgcctaa atcttttgac ggttgcccga atttcaacgc atagcctgaa
     2461 atagttttgc cgtctgttgg ttgtttttca ttttctttct ctactttggg agaatcggct
     2521 gttagctcag cttcttctgt cagccgtttt tccttttctt cttcattatt caactggtgt
     2581 aactccttcc tgttctttta attgatattt ctccaacgta tctaaatacg taaagttcaa
     2641 acttgctagt aaacgatcgc caccatccat ggggggtagc cccattttcg accgcccctc
     2701 attaatcgtg agaagtgacc ctttgacctg atccaataca ttttttactt ttgtttcagg
     2761 atcagtctct agtaactgat cggaattaaa gcggatattg gtttcaagtt tcgtatctaa
     2821 ttcactcaca aacacattaa agtaatgaat cagcgtactt tggatatact ggagattact
     2881 ttgaacggtg ctagaatgtt cattttcaac gcctaaacgc tcaatcggta caccaaacgc
     2941 cttggcaatt tgttttgtgt tccaatcatt ggaattgata agcttcaaaa catcggtgtt
     3001 tatttctaat gttttgtagt ccattgtttc atcaagaatg atcgttctaa gagcattatc
     3061 tccactactt gaaccgttgg cttcttcaaa cttttcacga attgcgttct ttgctgatcc
     3121 gtctaaatct gacttatgaa ccttcaaaat acctgaccca ctgacaccac gagtaaagaa
     3181 attgtgcaat gtgcggttgc cagcttgttg aattttcagt tcatcacgta acgcataaag
     3241 tggtggtaat cctgttaaac catcttgggt gaaatatttg aagtgcaaaa tatcgctaga
     3301 cttcacacgt ctttttttat caccaatctg ataagacaac gtaccattat ctaattgagt
     3361 aaccgttaca cttgagttag gtaataagtg aatttcttct accttttcac cgttgcgttt
     3421 aatctctgca aagctattac cgtttagcaa catattgacc gccaaagcaa actttaaatg
     3481 ccaaccatcc atttctgaat taggtttctc gtttagtaac ttcaccagtt cattatcagc
     3541 ttgtggcata ttgtttttga ccaattgaat cggacttgaa gcaatatcac tagcaataat
     3601 tcgaacggct gcaaacacat cactatttct taatgcacga acgcttgtaa agctggtggt
     3661 gtaatcatcc gattggattg ataccacatg ttgtaggaaa ggatctcccg ttgtattttg
     3721 tctcgcttta aaaaatgcca tcttttaacc tcccttctgt tctgtattta gcatgaccgc
     3781 taccccaata agcactatgc ccagcgctaa caagccatag tagacatttg ttagtaaggt
     3841 gatagcaacc aacacacaca ccaatccaag aagtaaaaga acggtatgaa tgtaattaga
     3901 aagaaaattc ttcacttgta taaaattcat tgtctgcttt cgctccttcc gttgtggtga
     3961 agtaatccat tgcaaacaca taggcattga ttaatgcagc tattggatca attttgttac
     4021 tgttctttga tttattaatc tgaatgccgt tgttatccgt ttttaaaata gcgttgttca
     4081 ctgcatgggt gagaatcgta tttttattgt gaataacgtt gccttcataa acttgttctc
     4141 taaaagttcg ggtcggaacg tttagagtaa tcgttccttg tcttacttct aacattgggt
     4201 agttggcttt ttcagcttta ctgataagcg aattagcgtt atacgggtca taacaaatcc
     4261 ccatacattc caaatcattt tcttgaatca aatcttgaat aaactgaaat acttggtcat
     4321 agtccacgat tccactttct aattgagtaa tggaacactc accagctttt tctagttctc
     4381 gataaggcaa accgtcgcgc ttttctttgt cttgaagccc atatttggtg gctacaaagc
     4441 tatgtgaatc acaataaagt tcgccgttat ctagcggtac gatccatgaa acgctagtta
     4501 aatcatctgt tttagataaa tccactccga tataaacggg cttgcctgta atatcttgtg
     4561 gttctacttc ggttgcctgc cagtcttcag caatcatata gctgtcctca ctcgcttgtc
     4621 tccataaatt gaagtttttc accaatacag aattaaggtt attttgtttc aaagcaaggt
     4681 tcacatcatc ttgaatggtt ggaatcatta cttttttgat ttcttcactt tcaaaaatcg
     4741 gattggcttt gatccatttc tcttgatcgt gaatttcttc gggatcgtcc aattcccata
     4801 tcgctataaa gtacctgtcg gcttgttctt cgccttttag cacacgatct aacaataagt
     4861 attcctcgta cataggaacg tttaaatcaa gcccagcggt actaataaca cacaataagc
     4921 cgttcttttg ttgggtcatt cctgacttaa tgacgttgta cgttttacga gtttttgctt
     4981 cgtgccactc gtcaagaatt gcggttgttg gtgcaaaccc atctagcgtg ctggtttcac
     5041 tagccagtgc catagcgaac gaattagagg gcaagtgctg aatcttagag ttcataattt
     5101 tcagttgtgg tcttaaaaac ttactagact tgaccacgtt tctaagtgaa ttagacagca
     5161 tatcatagcc caatttagct tgtttcaaag cgttacttac aaacaatact tgccttgctt
     5221 ctgctggttc ttgttccata atcaacgcat tggcagccat gccacttgcg atatacgttt
     5281 tcccattctt acggctcata ctggtaaacg ctcgattgaa tcgcctgtaa ttcccttctt
     5341 tagtacgcca gccataaaga ctgcctagaa tgaatttttg aaatcctaac atcttgattg
     5401 ctttaccgtc cgtagatggt agcaactcaa tgaatttaat cgctttggtg gcttgttctt
     5461 catcgaacac ataagggaag ctatctgact tagaacgctc caaatcgctt atatgacgct
     5521 cacacgcttg gataattttc ttgggtgcaa tcagcttacc tgataggact ttctcaatgt
     5581 aatcattcat gaattcatca actctttgaa cgggtcttcg ggttcatcat ttttaacctt
     5641 gttcaaagct agttttgatc gtgattctaa ggtcatacct agtgaggtgg cttgtttttt
     5701 caaatcactc atagcttgtg attgaacccg aaccgctgga ttggctttct ttactccgtt
     5761 ttcgcatgtg ataaacgtac ctgattttct tatctcggct tcggctgttt tcattcgagc
     5821 aaaggctaaa caataggcta ccaatgaacc ataatctgct tcactcaatg gataatcttt
     5881 ttttaatgtt ggtacgatac gattccattc acttctagcg ctcgctgcca tccagtcagg
     5941 gggcgtgata tttattaatg gttgctgctt gaacaattct tctctagcgt ccacacggat
     6001 tgctttttct tcacttgata tattgccttt tgtatcgtgt aatattttag ggtgtcccat
     6061 gttgtcctcc tttcagacaa aagaaaaagg aacgtaagca attaagctca cattccttcg
     6121 tttttcgatc agaatttttc tttatctaat ttatctatat aaatgatttt tccgttctgc
     6181 atttgaatgg tcacttgacc aaaatgaggt aactcaatgg tgcgctgttt ccctgtttta
     6241 tcagtaataa caattgtatt tttcaataaa tctcctactt tcattatata ctttttcact
     6301 tgtgattaca agtgtaatcg ttatttgtaa ttacccttat gccttttcac aacggttatt
     6361 tgtaccaaaa tgagtaggca tcgattgtca gtaagtttca cactatgccc ccttaactca
     6421 aagggtactc ttttctcgtc tttgtctttc attggctgtc ttggaattat ggtgtgcatg
     6481 gcataaagaa cgaaaattag tttcatctaa ccgtctattc caatcatctt ttagttcaat
     6541 tttgtgatct acaacatcag ctttccttat caagccttct tctaggcaat cctcacatac
     6601 tggatgattt aaccgataca agtatgaagc ctttcgccat gttctacttt gatagaattg
     6661 aaaatattta ccttcgcttt gctttctttc ttcacgctta attgccgttt gctttgcttt
     6721 atgcttccca caatacttct gcctataatc aatcaatgtt ttacatccag catgattaca
     6781 ttgtttttta actgccatag attaccaacc aaagcgatct cgttcgtcag aatcatcttg
     6841 ttcccatttt gatttgtatt caatataagg cgaaactatc ttcaagcacg ttccgtcttt
     6901 aaaattaact ttagtgattt tatcttcctc aaaactaata aagtctacat cttcttgagt
     6961 gtttgaatac tcaatgtgtt tctcaccatc gaagtatcta atactttcca tatgcataat
     7021 tattctacct ccaataaatt agttatcaat tcatcatctg ttagtagcaa agccaagaac
     7081 ttatcatgga tgtttaaaaa caaattatcc tcattcgatg taagctctcg ttgtctcgct
     7141 cgatcatata ggttaagcag acaattaaat tcatccatta gctcacctgt atcatcatag
     7201 ttgccactat ctaccaatga tcccactact agcgattgac accacgctac tgactcctgc
     7261 agccaatcag gttcttcttc cattatcttt aatacttctg gatcaaatgt ggaataatct
     7321 tctgttacca atcccacact aaatgccagc gtgttcatta gaaaccgatc catttcatct
     7381 gtatattgaa agttcggtgc tttgtttgtt gaaataatat tgagcattta ctttcctcct
     7441 tgattcgtaa cacgtggtaa cacctacgta acaggtaccc gttaccactc aatcccaacg
     7501 gggacaaggg ttctagcgtt ttggtaacag gtaacgggta aaatccagta acattattcc
     7561 ccagcatatt agctaactat ttcaaaagtt ttaaggaaat aaggtgttac cccgttacca
     7621 aattgctgta tcccttgggg cagtaagggt aaactagtaa cgggctaggt gttactgacc
     7681 cgttactacc cgttactttt tgtactttcc agctcgattt ttattttcat aggcatttga
     7741 cttattgtaa aatcctcgtc gtttatttct ttttggcagt tgcttccgtt cccaaccagg
     7801 aatactgcta ataatcaagg caatcttttt agtttctgca tctgatcgac cattcaataa
     7861 atcctttgct tgtttatcaa aaacagcttc caaaatatca gcggtgagta cgctatcaac
     7921 taaataaaac tcttgttgca tagtagctcc attatagaaa tgctgtaaat ttttctcatt
     7981 acatttactg tcaggatagt tctgataata agaacgtctc gcccacgctg gagcttcttc
     8041 ccatttatac gggatttcca tttccaaata tttagtgatt gcttctttaa ttggattctc
     8101 catcttggca tcctcttgat agtcttttgc aatttctaag acttctttat cttcatcggg
     8161 actaaaatag attcgttgtc ctttatcaaa aagcaccttt gcttcagcta acacttgaag
     8221 aaaatattca tcctttgttt taaacggatt catctttggc ttattcttac aaggcaaagg
     8281 ataaaaacgg cggtttcctg tatcatcttt caaatactgc ccatcattcg acgttccaat
     8341 aaatacacat tgccttgccc aagctatgac attccgacca taaggaggtc taattttatc
     8401 gatcttggca ctgatgaagt tttttatctg ttcaatttta gttttgttca ttgaagataa
     8461 ttctccaagt tcaataatta cattgccttg aagctgcata taatcatctt tcttttcacc
     8521 tagcgaatct aatgaatcgg tgaaaaaatc atccgtgtaa agtaatgctg gaacggtact
     8581 cttaccaatc ccttgcggtc catccaaaac tggaacgatc tcaaacttta ttcctggttt
     8641 atagatacgt gcgactgatc cgactatcca acgttttgtt acttctcgtg tgtaaaggtt
     8701 gtcgtctacc cctaaaaggt caataaacag tgtttccagt ctaggctgtt gatcccattt
     8761 aactttttcg atacgttctt ttactgggtt gaaaacattc tttctagcca catttactac
     8821 tgctttgtgt attttgttgt catcaaaaac tacattgtag ttttcttcaa tatatgccgt
     8881 taaggcatcc tcaaaactgt cttcccattc tccagcggta cgattgatcc aaggagaatc
     8941 ttttaatagg tgaatgtatc cgctaaattc gttataagca attgttccag ctaaattgct
     9001 atcattggtt aaaatcaatt ctgcattcac aacactgtta cttttaatct gatcgtcttt
     9061 attgcatctt aatcgtgaca tccaggtttt caattcaatg atattgtctt cacgctcgct
     9121 ggcttcggga aagtctaaaa tactatctaa tttatgctta tgagccatga gctttccccc
     9181 tcaaatcttt tttaatcatc gatcgaataa tttgattcac ttctttatct tttagcggtg
     9241 gttcattgta cgcatttgct gtataaatca attctgctaa agtttgatta tctacaccgt
     9301 gccatagtaa ccaaccgcat aacttagtta aatgatcgtt tctactacct tcgccagcgc
     9361 cttgaacaat catatctaat ttttttgctg tccctgtttt tgctacgggt cggtattgag
     9421 tctttatttc ttgcggtttt tttagcagta ggttcactag ccaatcggga gctggtgcaa
     9481 tattatttag agatacgttt tcaataggtt catatgctct cccaacactt cttgaaataa
     9541 tcatagttgg cgctattgga actcctttag tgataatatc aatcccgctt aaacttttac
     9601 ctttatcatc acgcccgaag atatcagcaa atgcacctgt ttcttgttgg ataattacac
     9661 catcaggaat tttaaagaag aaatggattc ctttacctgg tgtgatttcc gtataagtag
     9721 aaggaaatgg atcatacatt gaagcaagtt ttttataatt ctcaacgccg tttcgtgttt
     9781 cagaatgttg atctaggtca actaccacca tttgatgatc ttcaagcatt aacccaatat
     9841 ttctagttgc atcttccgtc caccattcat taatttgatt agaatcgtta gttgcatcat
     9901 tccaaccact agagcctttt attggaattt tagttccagg agataaggga taaattgacc
     9961 atcccgactt ttgtaattcc gttgctgctt gataattatt tgccaataaa cctctcctcc
    10021 tttttcaaaa tactggtata aatcattcag acacctccat gagcttcaac tgctcaagtt
    10081 cgtatttcaa cccatcccga acaaattcag caccttttaa ttgattctga tactttaccc
    10141 atgtattaaa tgttttcacc cttgcttctt gcatatcagt aatgtttaac catgcggtta
    10201 aaatctgatt ttttagctcg ttgatttctc gacaaattat tttgtgttct gcatgttttt
    10261 tgagtacata caaaactgtt agtttttcat tgaatgaatt tccaaagtag gttttcccga
    10321 tattattttc tagttcagcg atcccttgat taatttcctc taattcaagg ctatcgagtt
    10381 ggtttaatag ttttgttttg tacatctact tatgccctcc agttcttaat tgatagtcgt
    10441 actcgtcata agcagtgaag agatataacg ctaatcctgc aaagatagct atttttgcaa
    10501 ttaatggtaa taagccgaaa attccgccga ttaaaatgct tcctataatt aagtttcgtt
    10561 tcataatggt tccctccgat tttttaatat ggtataatcg gaacaaagaa tatcgggttt
    10621 tatcccaatt aagttgcctt tatcctgtcc gccaagactg ttatggataa ggctttttct
    10681 ttgttctcga tcatgttaat gctcctaagt tgtctgcaat tcagcagaac gattataaat
    10741 tttctcactc actgaaaact gcaacccata tttttcttta acagtcatta attctactgt
    10801 ctcatctaaa ataggttcac gatcaaccaa catttcagga gtcatatctt tcttttttac
    10861 cattttggaa tgaccataaa tagttgatac agctttattg gctatcacat tcgctttaat
    10921 gtagtctact ggttttggtt cagtaatagc gtggcttaat ttagtcattg cttctttttg
    10981 gtgttctttg tctagcatgc ggaatacttg aaagccttca agtcctgtgg actggcgtag
    11041 ttctttgatg atgtcgaaaa tccagtcttg aaatgcctcg gcttcttttt tacggctttt
    11101 gaatgccatg cggtaaatat tttttttcgt tgatgatgtt tacatcttgt gttctgccca
    11161 atgaatcgat gaccttacta gtagtaaccc catctttagg caaagagtga atggctctag
    11221 taacctgttt aagacctaac gcttccgcaa catctttcgc cactgcccac cattcatcat
    11281 taatatcgac aaatcttata atgtgtccgt tccaaatttc agttttcatt caacgtcctc
    11341 cagttcaagc cctagcaagc tcgctaacaa ctcaacttgc tgatacaaaa tttcttgata
    11401 ctcttcaata gaaagtcgtt ctttaaactc tgcttgtact aaatcgttca agccacgcaa
    11461 tactccgctt accgttggtt cttgtggtag caatttttga tagtccattt gttcgtacaa
    11521 acattcaaac gtttcttcta gcgctttgcc ttccaagttt gctaattctg ccgctgtgtc
    11581 ttctgtaaga attgttgata atactttttc gattgcttta ttttgtgtcg attccattat
    11641 tatttcctcc tattaatccc tttgccccca cggcttgcgt ttatttttgg tattcttcca
    11701 taaagcgatc acattcatct tgtgaaattc ttacgactcc atcaactata atcaacttta
    11761 aaccgttagg aacatatttc tgcatcatag tattgtatga aacgttcata tacttagcag
    11821 cttgtgcctt gttcatatat tttggaaact ggtacttttt ttctaataat gaaagtaacg
    11881 ttccttgagt ttgctctact tgttgaacaa ctcgatcgtc aataatttta aaagctggat
    11941 catcaagcgt gagtttgtca atttttaatt ccatatttat cccccctcgt tagcatacat
    12001 ttctatacaa agattttaaa aaaatgcaag aggctcttga atacatttct atacctctta
    12061 taaactcaat ataccatcat accttttcat ttgcaagaat tttaacatac ttttttgaac
    12121 ttttatgttt cacacatatt taaatcgtga tatagtagag aaaatatatg aatggtggtg
    12181 aaaatgtggc tagaaaaaaa taccccgaag cagtggagac aggacaaaga attagactgg
    12241 ctagaattga aaagggactc tcaatggaac aacttggagg taaattatcc ccaccggcca
    12301 gtaaaggtgc cgttagtaat tgggaaaatg gctataatct tcccaataac accaggctta
    12361 aacaactttc aaaggtatta gatgtatcca ctacctatct attaaacgga tcttatacat
    12421 taagagatat gcatatgatg cccgaaatta agcgaaatgt gcttgaaaag caattgaaaa
    12481 aaactattga aaaaaataac tctataattg attctaatct ttctaatgct atcaataaca
    12541 tcgaagaaga taacctgact gtctcacaga agatttattt aacaaacgca ataaaatatc
    12601 ttaaaacagg atctcgaatt gaatttcctg acgggagtac cccaattgct tatctcgcag
    12661 ctcttctaaa tacgctttta cgatatgact cattaattaa tgattcagaa aaaaatacag
    12721 atgagaaaaa agagtatcaa gaattttatg aaaatgatct tctaaaagag ttcacagctc
    12781 ttgttactga gctaaataaa cactattcaa aataataata aactgcctcc tccaaccgcc
    12841 cccacggctt gctacactat ggagggaaaa ctattatggc aacattcaag caatatgaaa
    12901 ctaaaaaagg taaattttgg ctctatgaag cctatcttgg aatgaacaaa atgactggca
    12961 aacctgataa ggttcgtaga cgaggttaca aaacaaaaaa agaagcccag cttgccttgt
    13021 cacgcttgca agtggactac gataaaaacg gtattaaaaa agcgaacaac gaaacgttta
    13081 aagaagttta tgacttgtgg ctagaaacat ataaaaccac tgtccgagaa gccaccttca
    13141 tgaaaactga aatcaagttt agaaagtgga ttcttccaaa atatggaaat cttcgtgtga
    13201 atgaagtcac ggtaaaaaaa gcccaacaaa ttgtaaatag ctgggctgaa actactgacc
    13261 aatacaaggt acttcattca acttctaagc gtatttttag ctacgctatc aatcttggga
    13321 ttattgagac taatccacta gacaacgtat taatgcctaa aagaaccacc actaagcata
    13381 aagaaaaggt caaagtttac agtaaagaac aactgatcac cttgtttgat tacctaaaca
    13441 gcaaacctga aacttatcga aacgactttg ataaaacgtt actacgcttt ctcttttata
    13501 gcggggttag gatcagtgaa gcccttgcat tgaattggtc agacattgat tttcaagaaa
    13561 atacggtcac catcaataaa acactaagcc aatccaaaaa tggctataaa atcagtgatc
    13621 ctaaaactga ttgtagtgct ggggttcttc cactagatga aagaacaatt tctatattaa
    13681 aaaaatggca gattaatcag cggaaatata tgctgacact tggtgtaact gatccgacta
    13741 tgattttttg tggtatctat aaagaactaa tcacacacca cgctatttat gcaagattga
    13801 ttactatttg tgaaaaagct atggttccat ttctcggtgt gcatgtagca agacacactc
    13861 atgcttcaat gctcttggac gctggagcta ctatggtaga agttcaaact agattacggc
    13921 atacaaaaat tagtcagacg atcgattgtt acggtcactt ggcaaaagaa acgaaagaaa
    13981 agactgttga aaaattagta caacacttaa atattgacta gatggcatac aatatggcat
    14041 acagtaaaaa ataaaaaaag gagaaaccct atcatatcaa ggtttctcct agctttatag
    14101 tctattcttc ttcttccgcc atgaatgtat tgaatacttc ttcaatcatg tcccattctg
    14161 cgtctgtttc gatttgttct aactcgcctt ctgttccatc ttcgttttcg atgtatgcat
    14221 aagcttgtaa ttctacatct tcatcttctg aaacaccagc aggatataaa agaacatagt
    14281 tacgtccaaa ttcttcttgt ccgtcaactg ttagtaggat ttcatataat gtttcatttc
    14341 cttggtcatc caccaatgtg atgtgttcgt gtccttcatg gtcgtgggaa tggttatgtt
    14401 ctgtcataag attacctctt ttcttttatt attaaaataa ttgcttacct gagccgtcta
    14461 aataattttg taagatcatc acagctgcta atttatcgat gacttttttt cgtttggctc
    14521 ttgaagtatt tgcctgctct actagcatac gttctgcctg aacagtggtt aatcgttcat
    14581 cttgataatc aactggcaag gagaatagtt ctttgatctt ttctccgtat gacatggaag
    14641 cttctgccct tggtccgatg gaattgttca tatttttagg taatccaact acaaaccggt
    14701 ctacttcata ttgtgctact aattccttca accgatcaaa gccaaactct tcttctgctt
    14761 cattgatccg aatgatctct actccttgag ctgtccagcc aaaggggtca ctgatagcaa
    14821 cacctactgt cttagaaccg acatccaagc ccattactct cataaattca ctccatgatt
    14881 tgagaggtag tccttgacaa ttacttccat gatctcatcg cgttcgtgac ggcgaatcaa
    14941 atttcgagca tcacgataac gaggaatgta ggacgggtct ccagaaagca agtagccgac
    15001 aatctgattg attggattgt agcctttttc ttccaaagcc cgataaacaa tcgctaatgt
    15061 ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt catctgtaaa
    15121 acccatgatt ctacacctct ttcccgcaag gtaattccag cttaccttct acaaccttat
    15181 tttactcgaa gaaagggaaa actacaaaca aatattccaa acttccgaat aaattaagaa
    15241 acactatttg tatgtttttt tccttttttc tgtagcttta tcaaaagtac tcttttcata
    15301 aagtatacgc tatatttctt tgaatgtatg tttttacaca attttttcat aatattcata
    15361 aaaaattcga atagctgcta ctaatttttt gtttttgttg cttgtcctgt ctcataattg
    15421 atcgtgtagc catcttgaac attgtagata aagacattga attcaatttt tttgtcttca
    15481 atgctttctg cttccaattg cactcccctg gctaccagtt cattcccctc aaaatgagga
    15541 gtgacacggt agcgtacatg atgatttgtt tctttgatat atgccgcaat atcattttcg
    15601 tgagcaagca tgtatggagc attcaacgaa cgtgtccctg tcatcaagtt tttaatgttg
    15661 ttattttgac cagtcagctg gaaaccaatt aaatggctgc gattatataa ccattgcccg
    15721 tcacttaatt tcttttgttt ccaacctgtc gggtcaatat acaaaggctc tctcttttca
    15781 gtagggaaca aatcttttcc aagcatagca ttggcttgtc ctactctgtt caatttatca
    15841 atatttgaat agctttccca tgttcctttg gaaaggctaa gatcctcttt tgtaaattcc
    15901 ggctgattcc cattgatttc tatttcttga tgttttccat catattctgg cacatccgct
    15961 acttgagaag cttgtggtgt ggaatcaaaa atagttgtaa tctgctgaaa gatatctgaa
    16021 tctttttgtg tggtggaaac accgaatgcc cctgcaatca cagcagcgac gatccccacg
    16081 atcgccttgg ttgttttttt gctcatttct ttactccttt actaacagct ccctaaatct
    16141 taacatatgt tcccctataa ataaatacta gaaagaaata gaaacagcaa aaaataaaac
    16201 ctgttttaag atggcttaaa aatacaaaaa agagacggat tcctcgtctc taatcagtca
    16261 ccactctatc tgtaaggtat ttattattat tcaaaaattt tcttcatact caagacaata
    16321 cactgatttc acaaccgctt ggtaaacggt gcgcaaaagc ttgcatctgc ggtaataagt
    16381 ccatccaagt taaaaacata agaagttgtt tttgcttggc tgttcttatt acttgttgca
    16441 gagcagcttt ttcacaacct attttaagcg ggtgacggga atcgaacccg cgacatcagc
    16501 ttgggaagct gggattttac cactaaacta cacccgcaat acaaaaacag tatacgcctc
    16561 ttttattcct tcctcaagtt tttttattct ttacgcttct ggtatgtttt gtagtaaaat
    16621 aaaactaatc aatcaattat tggagaagat gctatgacgg gaaaagaatt agatctgttg
    16681 ctggacactt gtttactcgc tgggaaaatc atgatggaaa gcaatgcgga gatgtatcgg
    16741 gtagaagata cgatgagtcg aattgcttta gcttctggaa atttccgttt ggtcagctat
    16801 gtcactcaaa ccggtctctt tattggtctt gatcgtacct cgaccatccg aatggaacaa
    16861 attaccaacc gttcgattaa tttagaaaaa gtagtaaaag taaataatct ttcacgtaaa
    16921 tatgttgctt cagagctaac attagaagaa ctttactgcg aactgcaagc aatcgaccga
    16981 gaccgttctt tctttcctgt ctggttgcaa atcgttagcg cggcagtgat cagtggaaca
    17041 attatggtgt tatttggcgg aaagatgacc gatttacctg tgactttagt cattggagga
    17101 ttgggatacg cagcttatct atatagtttg aaatttttta gaatcaaatt tttatcggaa
    17161 tttttatctt ctttattgat tggcatagcc gctatctaca gcgtacgttt tggcttagga
    17221 agcaatcagg atctaatcat tatcggatgt gtgatgcctt tagttcctgg agttcaaatt
    17281 acgaacgcta tccgtgattt gctagctgga cattatgtct caggagtttc tcgcggaaca
    17341 gaagcgatga tgactgctac gatgatcggt tttgcgattg cttttatttt ccagcttttt
    17401 tattaggaaa ggaacacaag tatggggacg atatttattc agttttcttt tagtttttta
    17461 gcgactgcgg catttgccgt tattacaaat gtacctaggc gttcattgat ttgttgtggt
    17521 ctttctggtg cttttggctg gatgatctac tggatctgtg ttcaaatagg cggatctgct
    17581 gcttttggtt cattgcttgg ttctttagga gtagcattca tcagcgatct tttctctaaa
    17641 cgtttgaaaa tgcccgtaac gattttcaat attcccggga tggttcctct agtccctggt
    17701 ggtttagctt atcaagcggt tcgaaatctc gttactgaat cctatcgaga agcggctcat
    17761 tatgcggtcc aagcaattat gattgctgga gcaatcgctt taggtcttgt attatcagaa
    17821 gtctttaacc ataacatccg taatttcaaa gtaaagcgtg acgcaatcat gatgaaacga
    17881 aaaaaacggc tttttaaata aacgaagtgt aacaaagaaa aggttgtgaa agagacgttc
    17941 agccttgagc gatgacaaaa tatctcacaa acattgcgac aagtaaccgc aggagcaata
    18001 agaagtaatt atgagtatta aaaaaagttt tgtaaaaata gcttctcaca tttttacata
    18061 aaattgactt aatactgaga aactgacttt tgaacaccct ctatctgtaa aaaaggggct
    18121 gcgacatgat ttttgtcacg gcccctcttc tttattcaaa ttttttccaa aacgttcgtt
    18181 gataataaac gatccataga gcggaaagta ttagaactaa accgatcaca ggaatattca
    18241 ctaaccaaaa tggataaaac aaagccaaca ttccgtataa gacgataaga ataatactga
    18301 tgatcaatga acccatcata gttgcgacta aacccaaact tcctgaacct ctgttaaata
    18361 attggttaat attggtccag ttcagcagca gcaaccgata atctctagcg aaataacgta
    18421 gtgacaataa gtagcagccg aataaagaac caaatagcaa gctagggata aaaaacaaag
    18481 gcagctgaaa agacagtccg cttaataatg cgatcccacc agttaaaaaa ctctgcaata
    18541 tccatccgaa tctgaacttt tcttttaaat attggttcat agagattggc aaagaacgga
    18601 taaacagaaa attttcttga tccaaagaaa taagattgct gataaaagaa gtctgattaa
    18661 ctgttaagaa agccaatgcg atccctacca ggaaaacaac accgattaac ttattatcta
    18721 aatcagttaa ttttatttcg cctcctaggg caaaagcaag aataaaaatg attggagtta
    18781 acaaggaaga agaaagaact tgcataatca aattaggttc ttttactaat tgagcattgt
    18841 aactaaataa aagctgatgc acattttggt tctttttatg ggttcttttt attttgaact
    18901 cactgtttga atcagtcagt tgctctgtaa gctttggtac tatcagctgt tttacagcaa
    18961 ggaaaaatcc cagagccgtg atagctagta ttccccaacc caataaacct tgttgagaaa
    19021 gtggctgact ggcagcatca tagaatggca ataatatcga aatcggatgc cgatctgtta
    19081 actcattttc aatagtcccc gtttgatgat tcatccacat gattccagca aatgcgatca
    19141 gcatcgagct gccaagcaag atactagaaa ctgtttttct atgtttttta aacactgctg
    19201 ttcgagccaa accaaaaaca atcaaactag ccagacaaaa aagaataatc aaaaatagag
    19261 aaaacaaaag gattgagata atgatagtaa gcaaaatccc tagatcagct cgccatccgg
    19321 ttaaaataaa catgactaga agcggaaaaa caaaaggaat gatggtcaaa gcaactacca
    19381 aaatctttgc tgaaaaaacc tgttcttgtc caaaaggcag aggcaaaaat acttgcaggt
    19441 catttccttc aaaaaagata ttataaatga ctgatatact ttgagttaaa cctaacatac
    19501 caaaaagtgc cacgtaataa gtaaagaaac ctggcaattt aacaaagttc atagcaaaca
    19561 tagcaaatcc gtaaatcagt aaaaaaatca gccctgaaaa aagattttga gacaaaagat
    19621 agatagttaa tcgctttccc ttttttcctt tctctcttgc tttattcgtg agctgtggat
    19681 taacatataa aagattaact cgaattaatt ctaataattg tcgcttatac atacggtcca
    19741 tctcctgtcg cttgccttcc agccattttc aaataaatct tttctagcgt ttcatcaggt
    19801 gagccattca ataattcact aactgccccc tgataaatca attcaccttt cttcaaaata
    19861 gccagtttat cgcataactg ttgtgcggta tccaatacat gtgtagaaaa aatgactgtt
    19921 tttccttttg cggcatgagc cttcatcatt tctttcaaat caaatgctgc ttgaggatca
    19981 agtccttgca aaggttcatc caatacccaa atatctggat ctggcagcaa cgtgccgatc
    20041 aaaatcgttt tttgacgcat tccatgtgaa aaacttgcga ttgtttcatt ttgatgacta
    20101 tacatatcaa aaagcgctgt caattctgct aaacgctttt cttttttttg tgtgtctaat
    20161 tcataggcag ctgaaatcaa gtcccaatat tctccagccg tcaattgtaa aaaaatatct
    20221 ggggaatcag gcacataacc gattttttgt ttgatactca atcgattttc cgtcaatttc
    20281 attccatcta ctaaaatcgt tcccgatgat ggttcaataa tactaactaa acttttaatc
    20341 gtagttgatt ttccggcacc attatgtcct aaaaatccaa aaatttcccc ttgtcttatc
    20401 gagagagaca agtcttttaa tgcttgtttt ttcccgtaac ttttacttac attgatgaat
    20461 tcaatcattt ctgtactcct tttgtttgtt attacttttt attgtaccct tcgttattgg
    20521 tggagtaaat gtcaaaaaga gtgcataaca aaaaaggata gaaacgacta ggctgctact
    20581 tgggttgttc tcttctacag gaaaacactc ttctaacagg taagatatga gtctttgttt
    20641 tctttaaaat gattcatttt ctatcttttc ttgcataaaa ttctttagtt tatcaatact
    20701 tctctttccc atctctagtt gttctccatt tgcaaatgtt actatttcgt ttactctgct
    20761 aatagatgta atgttttcag tattaatgat ataagctttt aaatctttac ataaatactc
    20821 atattcgagt gattttttat attttttaat tgtgccgttt acgagaatct cttcatcttt
    20881 caatactagg cctattttat ttttataacc tggcatgctc attagataaa ggatttcatc
    20941 gtaatggata tagtgatcgc tattaccata acggacagtt gtccaccttt ttttcgtagc
    21001 atgttgaaca ggatagtttt ctttttcttt caataagtaa ctttggatgc tttttttatt
    21061 tttttcataa gtgaattctt ttatcaaata accactaggc gtaatctttc gattaataac
    21121 agttattgct ttattcggat gagacgttag ataaacaatt ttacactttt cattcttttc
    21181 tctaagcatt tcgcctagtt cgataccgct ataataggcg ttcaaatcga tgtctaggaa
    21241 aaaaatatct gaggaataga tttcattatt tttaatgaat tcaacaaatt gtataacgtt
    21301 caatccctga acgatctcaa atttccaagg acttaataaa attctggatt cttccaaaat
    21361 ttctactagc attttttcaa aaaccaaact atcatccaaa ataaatatac gcgttaccat
    21421 aaaaagtacc tccactcgat attttactat caaaatcatt tcatacaact tttcaacata
    21481 catgttagga ataaattgta attctcttca caatcttttc caacaccttt gaaaccgata
    21541 tcaaatactt tcaggatgct gcttctaaaa taaatatcaa acaaatgaat acatcatgca
    21601 aaaaaagcca gacaaccgac gtccttgcgt gtcagctgtc tgagctatgt ttatttattt
    21661 tgttctttta aaaagaacga tacacatact gatttttcgg agcagagact ttctcagcgt
    21721 ttgtaacaac tacagatggg atttcacgct tgaatggttg ataatagtct gattggatgg
    21781 ttccttctat tttataccat tcatcatttt taaccgttac tccttccggc atatgcgtca
    21841 acaaaccaaa gactcctgaa tctgccacac aatggataat gccgaatcgg aacaagaaga
    21901 tatctgtatt gctatcgttc ggtgtttggt aaacaaatcc ttcatagctg atttttttgc
    21961 caatgaattc gctagggtaa ttataaatta gctccataac ttctaaatag ttttcatctg
    22021 tgatcttgat agtttgctta tccccatact tttctttcaa ctcattcatt tggtctaagt
    22081 agtctgattt gttgaaataa atactggtat ccggctttaa gtattgtgtc tgcatctccg
    22141 gatcccctac tgattcttta cttactggga aattaaatcc tttggcttct acgatcgtag
    22201 tgtcgagact aactgttgga aacaacgtcc ccacgattac tggcaaagct agcagtacat
    22261 aagcgattcc acgttgataa ggttttgaca acccatggtc atgatcgtgc tcgtgtgcct
    22321 tttcttctgc ttttccgttt ttgacccaca aatacagctg aactaaagcc aaaagagcag
    22381 ataaaatcat tgaaagaata gctaagtagc gataatggac attgatatat tgatcgagtt
    22441 tgccagaaac ttgcaagtac atcatcaatt cgaaataccc agataaaatc aaaaaccgca
    22501 gcatattttc cctcctaaat aaataatgtg aatccaataa cgctaagggc aatcagtccg
    22561 atcagcgata aaataaagcg tcctgtaaaa tatcgtttca tcatcagtag atttttgata
    22621 tcgaccattg ggccaaagac caaaaaggca gctacaggtg ctgtgccgaa cagacttaac
    22681 aaagaagatc ctataaacgc atctgcttct gagcaaagag acatcgtaaa ggccaataac
    22741 agcatgatga taatggctaa aatctttgtt gagccgagcg ttagcataat tctagttggt
    22801 aaataagtct gcatcgcagc tgcgattaat ccgccaatga tcaaataacg acctgtatca
    22861 aaaaattcgt cgattgaatg tgtcaacaca tgcccagttt gatggatcag ctgatttttt
    22921 ggttcattct tctcactttg ttcttcttca tggacatgat gatggtgatt ttctgctact
    22981 ttagacgtga ttttttcttt caatacagcg tctttttgaa aataggcaag ccagacacca
    23041 atcacaaggg caacaaaaaa gctgcctatc acccgccagc ttgccatctg ccaagaattg
    23101 ccaaatgcga taaaagtgga aaaaatcacg attggattga tgatcggagc agtcagcata
    23161 aaagcaaacg ctgtgtgaac tggtacgcct tttttaacaa attgatgaac gatcggtacg
    23221 atgccgcatt cacatgaagg aaagaaaaat cctaagatcg aaccagtaaa aatagagaag
    23281 atacgatttt caggaagcca tctcttcaca cgttctgggg tcaaaaatac ttgtaaggcg
    23341 cctgagataa gacagcctaa taatacgaaa ggtaatgctt caataacgat cgataagaaa
    23401 atcgtactca tctgcaaaac cgaatttgga ataaaagaaa acatgagttt cctccttttt
    23461 atttctagtt cattcgcttt gcaaatatac gcttttattc aaaaaaaaca agttgctctg
    23521 taaattttta cacaactctt attttttacc aaagcacttc aattgacttt ttctgttatt
    23581 cccattaaat taaggtctca tacatatcac ttctatcata aaagggagaa taaaaatgaa
    23641 acaaaccact aacttgactg aagttcaaga catactgcaa cagtcatccg ttgccgtcat
    23701 ttatctttcg atgcctaatt gcagtgtttg tcacgctgtt cgtccacgac tagaggaact
    23761 tctcacacat tatgatattc cggcacttca tttggatgca tttgaaacac cagaagtagc
    23821 tagtaaattt gaagtgctga ctgcgccagt tgtcctgatt tttcatcaag gaaaagaagt
    23881 ctttcggcaa gcacggttca tcgactttaa aaaaatccgt tatctactag atgaactgac
    23941 agcagaagat gattcccttt cttatgaaga aatcttccga acaaaataat cgttgtccaa
    24001 acagaaaaaa gcgattgaac aagtgaaagt tttgccttca tttgttcaat cgctttttat
    24061 ttttattctg ctgtattttc ggaatgcgca tttgcgtcag aatgcaagaa atagatcaag
    24121 gtttgcaatt cattcgtcaa atcaacattt tggatcaata catctgacgg agcattcaat
    24181 cgagcagcgg taaagttcaa gattcctttg attcctgctt ccgttaactg attgactact
    24241 tcttgtgctc tttcagcggg tatcgttaaa attgctactt cgatttgttg gatcttgatt
    24301 tgttctgaca tatcttccat tggataaacg gggataccgt ctacgatgcg cccaacgatt
    24361 tcctcattca catcaaaagc acaactgata cgtatgctat tactttgatg gaatttgtat
    24421 tttagtaaag cacttcccaa gttccctaca ccgatcagcg caacatttgt caattgatcg
    24481 tcgtttaatg tctttgcaaa aaagtgcata agatcttcta catcataacc atacccacgt
    24541 tttcctaatt cgccaaaata agaaaaatct cgacggatcg ttgcactatc tacttgaacg
    24601 gcttcactta attcagttga agacactttt ttctttccag cattatttaa aatccttaaa
    24661 tagcgataat acaaaggcag tcttttagct gtcgcctttg gaataatatg atctttcacg
    24721 aaatgacctc cacgtattgt ctaattacat ctttgatttc tccatcatag cagtatttat
    24781 aggaaaaaat caattccatt gctcagatat tgtgaaattt tattctttat tcatttattt
    24841 atcatttctg caaatctcgc agtataatgg aatttatggt agactactaa ttgaaaaata
    24901 gttttttatt tgaggagaat tcacaaagta gaaaagagga ttccatgata ttattacaag
    24961 ccaatcagat tgctcgccat tttggtgcag aagtcttatt tgaaaacata catttagaaa
    25021 tcagtacagg cgctcgtgtc gctttagttg gcagaaatgg cgctggaaaa tcgactttat
    25081 taaagatcat tgctggaatt gaagcacctg atgaaggaaa gatcgctaaa aataaaacag
    25141 cgactctcgg atacctcgct caggacacag gacttgattc caatgaaaca gtttggaacg
    25201 agatgctgaa agcttttgaa caagttagac aaatggaaga gcggatgcgc gagctagaga
    25261 ttttcattag cgaaggtacg ccagacacac ctgcttatag ttcttattta aaagaatacg
    25321 accggctgca acacgatttt tcagatcgaa acggctatgg ttatgaaaat gaaattcgtt
    25381 ccgtattgca tgggtttaaa ttcgattctt cattttatga tcagaaaatc gatactttat
    25441 caggcggaca gcgcacacgt ttagctttag ctcgcatgct tttgcaacac cctgacattt
    25501 tgattctaga tgaaccaacg aaccatttag atatcgagac actttcctgg cttgagaatt
    25561 atctgcaaag ctatactggt gcattattga ttgtttccca tgaccgttat tttttagata
    25621 aagtagtcaa tgaagtctat gaattaagca gaagaaaaat gcgccattac aaaggtaact
    25681 attcgaaata tctagatttg aaagcagaac agttagcaag tgaatggaag gcttatgaaa
    25741 agcagcaaac agaaatccat aaattagaag acttcgttgc tcggaacctt gttcgcgcct
    25801 ctaccacaaa acgagcgcaa agccgcagaa agacattaga aaaaatggac cggctggatc
    25861 gtcctcaagg ggatgaaaaa caagcaaact ttctattcag tatagaaaag ccttctggaa
    25921 atgtagtctt gcaaatcgaa gatgccgcta tcggatatga taatgtcacc ttatctgaac
    25981 caatcaatct agatatccgt cgtcaagaag cgattgctct agtaggtccg aatggtatcg
    26041 ggaaatcgac gttgctgaaa tcaatcattc accagatccc ttttatccgt ggaaaagaaa
    26101 cgctagggac aaatgtcacc atcggctatt acgatcaagg tcaagctgat ctgcatacaa
    26161 acaagacgat cctagcagaa ttgtgggatg agcatcctac cactccagaa aaagagatcc
    26221 gaagtgcttt aggtgctttc ttattttctg gcgaagatgt agaaaaacca atccctctgc
    26281 taagtggcgg tgaaaaagca cgggttgctt tagccaagtt agcgatgaac aaagaaaatt
    26341 ttttgatttt ggatgaacca acaaaccatt tagatattga caacaaagaa gtattagaaa
    26401 atgcattgat cgattatgac ggcacactgc tttttgtttc ccatgaccga tatttcatca
    26461 atcggatcgc gacaaaagtc gtcgaactct ctgaaaatgg cagcaaagtc tatctaggag
    26521 actatgatta ttatttagag aaaaagcagg aagaagaaga aatcgccgaa ttgttggcac
    26581 aagatgagcc agaatctgct cctgctccca cgaacaaatc tgattattac caaagcaaag
    26641 aacagcaaaa gcttatccgt agccttcaac gaaaaattac tcaggttgaa gaagaaatgg
    26701 cgcggatcga tgaatggatc gatcagttgg aacaagaaat ggtcgcgcca gaaaacctgg
    26761 aagatcacgt aaaactcaat gagctgaacc aagatttaga agctgctaga caagatcaag
    26821 aaacaaaatt gacggaatgg gaagaactaa gcttggaatt agaagaatat gaaaattaac
    26881 gaaaaaacca aaacagaaac cattcatgct gttttggttt ttcttttttt attttatcaa
    26941 acaaaaattc catatttacc tttttcacca ataagaagac caattactag tcattctcac
    27001 taaagcttct aaataatagt aatcacccca aatacaaggt gtatttaccc cattattttt
    27061 gggcttatta taaacagact gtgttaaaaa gccatcttct tcttccgttg tagcatatct
    27121 ttctttcaat tgtcgcataa ttttccaaac agcagactgg tataagttct tttctgtttt
    27181 gtctaataga cctgattcta ttaattctaa taatccacaa accatgatca ccatactaga
    27241 tgtatctttt tcttctctgc tagaatcatc aaagatcaaa tcccaataac aaatggtatc
    27301 ttctggcaac cgattcataa aataattggc taatattttg cttaaatcaa aatatttttg
    27361 ttctttcaaa taacgataac ttaatgcaaa accgtaaatc ccccatgctt ggcctctagc
    27421 ccaacaggaa ttatcactat aaccttgatg cgtacttcca tatcttggtt ttccatcttt
    27481 gacatccata taaaatgtat gataagttga accatcttct cttactaaat aatctgctgc
    27541 attttgaata tgagcctgag cagctttttt atatttttga tttccagttt cttcactcgc
    27601 ccaatacaat aatggaagat tcatgcaaca atccatgatc attctacctt gttgtgaagg
    27661 atcactagga tctccccaag cttgtataac tcctgccttc tcccaaaaac gctgaagtaa
    27721 tgtttctgca gcatcaattg aagcttcctt catttttcga tttcccgtta ttttataatc
    27781 tgctacggtt gataaagaat acaaaaaacc aagatcgtga tgattagtga taatttgaca
    27841 gtcaatcctg tggataaaat ctatatctgc tttttcagct gtttgttgaa ataaaggatc
    27901 acccgtcttc tcaaataaca accaattaat tcctgtccaa aatccttctg tccaaccaat
    27961 attgtctctt aatggataac gtccttttat aggacactcg cttgggaatt gatactgact
    28021 gttactgata cgtttgatta acctctccgt gtgtctgcga caacgctcga tctcattatt
    28081 ccattctttt tccattaatt ctttataaca ttcatttatc catttttctg gatgtattat
    28141 ctcttcaaac atcactttat caagcatttc cccaccagcc tttagcataa ttttagtctt
    28201 tttttattgt ttttattttg gatagttagc ccggttgtcc catcaaaatc aattttggga
    28261 cgattgttcc atacttctaa actataatcc cgatcttgat gagcaaccac tcctgttccg
    28321 agccaatggg ttccttttcc aaatgtaccc tctaatccag gaataaaagt gttttcccat
    28381 gtcatcaagt ttgtattggg caaacccata atagctgtgg attttcgttt aatagtatcg
    28441 tcacttaaac agataatacc tgaaaagcca gcttcactca ccctatagtt ttccttgtct
    28501 tcttctatga ttattccttc tttctcagga gaacattcaa tagcaaaccc tgtctcatat
    28561 agaacatatt ctgtatctaa ttctactttg tgtacgcgaa tctgccagcc ttcaaaagga
    28621 ataagccaag ttctgatttt tgcctgtaaa gtaggttccc atgtagaggc aagtacctct
    28681 ttcttacata caatgtctcg tacattattt cgaacgatga actgtccata ggttttgatg
    28741 ctctcattct taaaactggt cgtgatatat gtgtccgctc gctgaatccc tagcattgaa
    28801 tccattgcca tttctgcttt caattgattc gctcgcggta tcgaaaaacc aaattgacta
    28861 gaataagcaa attttaaata tttttcattg gtcaatgtat ggaaatttgg ccctaattgt
    28921 cccgcattca aaaaaaatgt atgtccatta tcatgttgta tgagcaaatt ggcttctgaa
    28981 agaagtttag ttccttcacg aataggcata ggcatttctt tagccgtcca atactcatgg
    29041 tcttcaggta gcgccaacaa taaaaaaatc ttattcagcc ataatggaga taacgtacta
    29101 ttatagggtt cagtcaatgt caattgaggg taggtataac caatattcag gatacctttt
    29161 tcatcaaata tttctttttg catccaccat cttatattcc gattgactaa ccccttgatt
    29221 tcacctaatt taaaaggcaa aagatcggcc cacaacatag ctgaccaaaa gcttgaaact
    29281 gcaaaacgat aaatcatgct tcgaccaaaa ggcacatttg caccatcttc cgcaaaaaaa
    29341 tgaatatagt ctctagaaaa atcaatagct cggttcttat atatctggct aatgggcttt
    29401 tctggttcta gtacgctata aatcaaacca taataatgaa atgcaaaagg attataataa
    29461 tcttggcgac ctcttgaact gtctcgatac cagccatttt gaagataaca attttctata
    29521 ttatcaaatg cttctttcag tgctgattct ttaactggat aaccaaattt ttggataatt
    29581 gttccgacaa ttatcttaaa aaattgccaa ttaccatctg cacattcata ttcaaaaatt
    29641 tgcgagaacc aatgaataat ctggcttttt tctcctgtat ctaatttttt ccataataat
    29701 tgttcatgat gatataacgc taataacatt gcgggcattt ctaccatacg ctggtctctt
    29761 tcatgtattt ctccccaata atcgggatgg gaaggatctg ttccagcttt tattcctgat
    29821 agaattaatt tcaaccatcg ttcatctaat tggtcaattg cgggccccgc tccccataaa
    29881 acccgagaga atccttccat tcctgcaatt ttatttccat atcctacccc ttgatgtata
    29941 taatcaattt gtgcgccact tgctcgataa aacggctgga gtggttgatt tatcttttta
    30001 aataagtcaa agtaatcctg tctggtcctt aatagtggta ctggatgttt cattgtattt
    30061 tctcctcttt ttactaccaa atagctaatt ccgactgttc taatcgaagt aagccttcaa
    30121 taaaaaaata atcagaatag atcagtggca cattataagt cagttctcta tggtatgctt
    30181 ctgctccgcc tccaatgatc ccatcttcat ccatattcca atttgcatag tgcttttctg
    30241 tcttttggag tatattgata gcagcttcat gatacatttt tgatcgtaca gaggaaacat
    30301 atcctgctaa ttccagaaga ccgcatgcag cgcatactcc agcactggta tcaatattcg
    30361 aatcttttac agcaaaatca atgagaggaa tataaccagt tttttcgatt ttctgaataa
    30421 aatttactgc tatcttttct gctatagcta gatagtacgg attttttgtg taacgataac
    30481 ttaacgcgaa tccatataaa gcccatgcat ttcctctgct ccaacaagaa ttctcattgt
    30541 atccttgccc gccaagtaac cgaatatatt tccctgattc tgcatcaaat tctgcgatat
    30601 gcccggttga tccatctttc ctcactagat acttagctgt tgtatctgca tgctgccgag
    30661 caattttaga gaacctcgga tcaccagttt ccctcgaagc ccaatacagt aatggtaaat
    30721 tcatcatact atcaataatc atccaaccag gtttatggtc attccatgct actaaatagt
    30781 tccctgaaat attatagcgt ccagcaagta ccgtagctgc atgtaaccct agttgtcgac
    30841 tatgtttgtt ttgagactgt tgataatgtg caatagccga tggtaagaat aaaaaaccta
    30901 catcatgatg aacgtctgaa aaatggtaca gggcagattc taataattgt tcttgttgta
    30961 ttgctgcctc tttgaacttt tctcgttttg tcttgtgaaa gagctgccat aaaataccac
    31021 tccaaaaacc attggtccac cagtcgattc ctctgtcctt tattacgtct gtatattttc
    31081 cgttgtaaga aatatatgga attttattcc ccattctgtc taattccgca tctatctttt
    31141 cagaagctac attcaaaatt tttcttgacc attccttcgt ctgttcattc atggatatcc
    31201 cgccattctt ttaattttta tgaatgtgga gttgctccta gaacgaacta gatggttaac
    31261 attcgttatt ttttttactt atttgcagct ccaatgtcat tctccttaaa tttagactgc
    31321 gcccacacat aaatcaagac cattgggatc actgaagttg ctaaaattag cataattgcg
    31381 ccataattaa ttggctcacc tgtactagaa accgtcatat ttttgataat taagggaacc
    31441 gtatatttat ctttatctga aagcatgact aatggaaata gatattgatt ccaacttccc
    31501 ataaagataa gtaaaccact agtaataatc gtaggtgata aaatcggtac agcgattttc
    31561 gtaaaaatca cccactcatt ggcaccatcg attctggcag cctccatcag tgctgtagga
    31621 aattgactgc tatattgtcg catcataaac actgaagttg tactggcaat cgaagggatg
    31681 atcacacctg cataagtatt cgataatcct aaacgactca taatgtaaaa taaaggaatc
    31741 aatgtggtaa aaaaaggcac catcctagat agcataactg tattgaacaa cagtttattc
    31801 cccataaaac gatactttgc taatgcataa cccgccattg tagaactggc tgtagctaaa
    31861 actgtactga ttacagcaat aacaatcgaa ttccaaataa tcaagggata attatatctc
    31921 gccatgatgg cttgccagtt ttttaatagc atatccccaa acttgaaatt gattccgcca
    31981 cttaaaattt cttgattact ttgcgttgaa gctacaatca tatataaata tggcatcaaa
    32041 aatattaagg caccaaacac aataatgacg tacgtaaaaa ttctgctttg tatcttttgt
    32101 ttagtcattt ttatcacctc cgaatttaaa ttgaatagcc gataatataa cagatacaaa
    32161 caccatcgtc caggcaatag cagaggcttt tccaaaatca accatttcaa aacttgtttt
    32221 atataaattg actcctacga ccaaagctga attttctggt ccattggaag tcccaaagaa
    32281 tatatttggg atattgaaat tacccaagcc attgatagtg gctaaaactg tggccattaa
    32341 aatgattggc tttagcatgg ggatcgttaa taaaaagaat tgccgaaatt tcgatgctcc
    32401 atctatttca gcactttcgt atatctcttt agaaatccct tgaagaccag ctaaaaataa
    32461 aatagtgtta tatcctacac taagccaaat catcgtgatg atcattgata ttcgtatcca
    32521 aaaaggattt gttaaccact gtacaccgtt aattcctagc gcattcaaaa atccattgaa
    32581 taacccagtc ggattgaaaa aagtcatgaa tacggaagct acagccaccg ttgatgtaat
    32641 ggttggaata taatagacag ttctaaaaaa tccttttcct tttaactgtg ctgaattcaa
    32701 taaaacagca aagtaaatag ctaatagcat cgttattgga attgatcctg ccatgataat
    32761 caaaacattc gtgtaagatt taataaacaa tggatctcgg aagatagcta gataattgtc
    32821 aaatccaaca aattcaaatg agtagccaat tttcataaaa ctaaaagcaa acgagataat
    32881 gatgggaatc aaaatcgtca caataaagaa aataacaaaa ggtgtgacca atccataagc
    32941 acttttttta tgagacgtat ctgttgcttt tttcttgctg ttctttattg tcttgcttgg
    33001 taacgaagat tgcatcatcg ttaagtcctc ctaatatttt ttaatcacca gttaatttta
    33061 cgccgtactt atgagcaaaa ttttctgctt cttttagata tgaattttca aaatcacctg
    33121 tttgccaata gttatatgca gcggtagtca aataagacgt agcatctgaa taagtggctg
    33181 gtaaaacagt atttgatgta ttttcattga ttttattcaa tagttcatag tatttttgtc
    33241 ctccttagta gtcaaacttt ttctcagctg cttctgtttg ataggccaat gagttagatg
    33301 tagccacgcc cagctccagt gcgttggcta agcattcttc atctgtcagc ataaatgtaa
    33361 gaaattgttg tgccacttgt ggattttttg catctttagg gacataaaaa cttgatcctc
    33421 ctgatactgc accttttcct ggattttctt ttgaaaaagg aatcagtgga gccatgcgcc
    33481 atttgtcaga tgcatcagga aaattcaaag acatattcgt agcataccag ccgccagcaa
    33541 caaacattgc agattcttga aatgctttct cagcatcttg tgcaccgtag aaatgtacaa
    33601 tatctgagtc gatcatttct ttgattaact ttgcagcatg tttcgcttct ttagtattca
    33661 gacggatatt tccttcttca tcaagcaaag attgattctg ctgggctagc atgttgataa
    33721 acaattcttg gtccccagat tctggcaagg caattccaaa actaccagtt ttttctttta
    33781 atgcttttgc cacctggata tattcttccc aagaactgat ttcttcaaaa tctacaccta
    33841 tttcagcaaa tttttttggt tgataataac aaagtgctgg ggtaaaatca ctagcccaag
    33901 gaataatcgt ttcattactc gtttgttgac gcaaaatatt caatttttga tcataaaact
    33961 gatcgccata tttgtcataa aatccatatt catcagcact ataaaaagca tcttgaaatt
    34021 tttccagcac acctgctgct gaagtgtctg ccatggagat aatatccggc atatcttcgt
    34081 gtgttaccaa ttttggggtg atcatttgta ctgttgctgt tcccgaagct acattcacaa
    34141 tttttaagtc aatatttgct ccagttttgc ggttatagat ttttgcagca ttttcaacat
    34201 agtttgtcag tttgccgttt gcgacatata cttctaatgt agtggctttt tttatcattg
    34261 gatctgcgat gtttgctgtt acttcttcgt tagtttttcc acatgcactt aagctgaata
    34321 atagggtaat gtttagcaaa gctatcacca atttgtatct ttttttcatg tctatccctt
    34381 ctttcttttc aagttcttat ataaaacgct tt
//
LOCUS       DAYYAR010000017        33751 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000017 DAYYAR010000000
VERSION     DAYYAR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.017"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..565)
                     /gene="frr"
                     /locus_tag="ACHAT0_000689"
     CDS             complement(8..565)
                     /gene="frr"
                     /locus_tag="ACHAT0_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8450654.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(568..1290)
                     /gene="pyrH"
                     /locus_tag="ACHAT0_000690"
     CDS             complement(568..1290)
                     /gene="pyrH"
                     /locus_tag="ACHAT0_000690"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8450655.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1426..2307)
                     /gene="tsf"
                     /locus_tag="ACHAT0_000691"
     CDS             complement(1426..2307)
                     /gene="tsf"
                     /locus_tag="ACHAT0_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8450656.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2406..3188)
                     /gene="rpsB"
                     /locus_tag="ACHAT0_000692"
     CDS             complement(2406..3188)
                     /gene="rpsB"
                     /locus_tag="ACHAT0_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8450657.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3547..4026
                     /locus_tag="ACHAT0_000693"
     CDS             3547..4026
                     /locus_tag="ACHAT0_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8450658.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4243..5334
                     /gene="phnW"
                     /locus_tag="ACHAT0_000694"
     CDS             4243..5334
                     /gene="phnW"
                     /locus_tag="ACHAT0_000694"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM8450659.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5327..5455
                     /locus_tag="ACHAT0_000695"
     CDS             5327..5455
                     /locus_tag="ACHAT0_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8450660.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5459..6004
                     /locus_tag="ACHAT0_000696"
     CDS             5459..6004
                     /locus_tag="ACHAT0_000696"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8450661.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6458..8149)
                     /gene="argS"
                     /locus_tag="ACHAT0_000697"
     CDS             complement(6458..8149)
                     /gene="argS"
                     /locus_tag="ACHAT0_000697"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8450662.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8569..9516)
                     /gene="arcC"
                     /locus_tag="ACHAT0_000698"
     CDS             complement(8569..9516)
                     /gene="arcC"
                     /locus_tag="ACHAT0_000698"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8450663.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9631..10650)
                     /gene="argF"
                     /locus_tag="ACHAT0_000699"
     CDS             complement(9631..10650)
                     /gene="argF"
                     /locus_tag="ACHAT0_000699"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8450664.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10741..11970)
                     /gene="arcA"
                     /locus_tag="ACHAT0_000700"
     CDS             complement(10741..11970)
                     /gene="arcA"
                     /locus_tag="ACHAT0_000700"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8450665.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12431..13132
                     /locus_tag="ACHAT0_000701"
     CDS             12431..13132
                     /locus_tag="ACHAT0_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8450666.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13741..14757)
                     /gene="tsaD"
                     /locus_tag="ACHAT0_000702"
     CDS             complement(13741..14757)
                     /gene="tsaD"
                     /locus_tag="ACHAT0_000702"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8450667.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14754..15218)
                     /gene="rimI"
                     /locus_tag="ACHAT0_000703"
     CDS             complement(14754..15218)
                     /gene="rimI"
                     /locus_tag="ACHAT0_000703"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8450668.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15225..15767)
                     /gene="rimI"
                     /locus_tag="ACHAT0_000704"
     CDS             complement(15225..15767)
                     /gene="rimI"
                     /locus_tag="ACHAT0_000704"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8450669.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15751..16473)
                     /gene="tsaB"
                     /locus_tag="ACHAT0_000705"
     CDS             complement(15751..16473)
                     /gene="tsaB"
                     /locus_tag="ACHAT0_000705"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8450670.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(16664..17644)
                     /locus_tag="ACHAT0_000706"
     CDS             complement(16664..17644)
                     /locus_tag="ACHAT0_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8450671.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(17668..19152)
                     /gene="galT"
                     /locus_tag="ACHAT0_000707"
     CDS             complement(17668..19152)
                     /gene="galT"
                     /locus_tag="ACHAT0_000707"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8450672.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19164..20153)
                     /gene="galE"
                     /locus_tag="ACHAT0_000708"
     CDS             complement(19164..20153)
                     /gene="galE"
                     /locus_tag="ACHAT0_000708"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8450673.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20401..20568
                     /locus_tag="ACHAT0_000709"
     CDS             20401..20568
                     /locus_tag="ACHAT0_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8450674.1"
                     /translation="MEKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(20630..22441)
                     /locus_tag="ACHAT0_000710"
     CDS             complement(20630..22441)
                     /locus_tag="ACHAT0_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8450675.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(22438..22803)
                     /locus_tag="ACHAT0_000711"
     CDS             complement(22438..22803)
                     /locus_tag="ACHAT0_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8450676.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(22966..23361)
                     /locus_tag="ACHAT0_000712"
     CDS             complement(22966..23361)
                     /locus_tag="ACHAT0_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8450677.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(23379..24341)
                     /locus_tag="ACHAT0_000713"
     CDS             complement(23379..24341)
                     /locus_tag="ACHAT0_000713"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8450678.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(24341..24553)
                     /locus_tag="ACHAT0_000714"
     CDS             complement(24341..24553)
                     /locus_tag="ACHAT0_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8450679.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(24574..25272)
                     /locus_tag="ACHAT0_000715"
     CDS             complement(24574..25272)
                     /locus_tag="ACHAT0_000715"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8450680.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(25292..25834)
                     /locus_tag="ACHAT0_000716"
     CDS             complement(25292..25834)
                     /locus_tag="ACHAT0_000716"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8450681.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(25966..26970)
                     /locus_tag="ACHAT0_000717"
     CDS             complement(25966..26970)
                     /locus_tag="ACHAT0_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8450682.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(26967..27956)
                     /locus_tag="ACHAT0_000718"
     CDS             complement(26967..27956)
                     /locus_tag="ACHAT0_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8450683.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(27953..28759)
                     /locus_tag="ACHAT0_000719"
     CDS             complement(27953..28759)
                     /locus_tag="ACHAT0_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450684.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            28925..29881
                     /locus_tag="ACHAT0_000720"
     CDS             28925..29881
                     /locus_tag="ACHAT0_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8450685.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(29958..30476)
                     /locus_tag="ACHAT0_000721"
     CDS             complement(29958..30476)
                     /locus_tag="ACHAT0_000721"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8450686.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(30564..30713)
                     /gene="rpmG"
                     /locus_tag="ACHAT0_000722"
     CDS             complement(30564..30713)
                     /gene="rpmG"
                     /locus_tag="ACHAT0_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8450687.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            30941..31387
                     /locus_tag="ACHAT0_000723"
     CDS             30941..31387
                     /locus_tag="ACHAT0_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8450688.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(31582..33477)
                     /gene="asnB"
                     /locus_tag="ACHAT0_000724"
     CDS             complement(31582..33477)
                     /gene="asnB"
                     /locus_tag="ACHAT0_000724"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8450689.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         9831 a         7088 c         5905 g        10927 t
ORIGIN      
        1 gattttatta aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt
       61 tgatgctgtt atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg
      121 taatatcacc atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac
      181 gaatcgcgat ttttgcattt tctgcttctt ttttaacatc tttagctaat tcttttctgc
      241 gttcttctgt cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga
      301 ttccgatatc acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag
      361 gtgtgatcat taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg
      421 ttggcgcacc gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg
      481 cgcggatttg cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg
      541 cttctgtcat aattgcatct gccattttta tttccccctt acagttgttc cgatattttc
      601 gcctaagatt gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat
      661 atcattatcc atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat
      721 aacttccaga tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc
      781 agaataaaca ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc
      841 cgcgcgtaat gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa
      901 gataacgatc cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc
      961 gatttgacgc atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt
     1021 atcctgtaaa gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc
     1081 acgttccatg cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac
     1141 gattgccatt tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat
     1201 tactggtggt ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa
     1261 gactacacgc tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat
     1321 tttaccatac ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa
     1381 aagggagcgc acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg
     1441 gctcataact tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa
     1501 acgtacgaat gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc
     1561 aggatcttta acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg
     1621 tccttctacc attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt
     1681 aagaacagtt ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg
     1741 aggattgata gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc
     1801 atctagaaca gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc
     1861 attgtcgtct ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg
     1921 agttgcttcg atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc
     1981 ttccatgtct gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg
     2041 gaacatttcg tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt
     2101 gttacctttc acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc
     2161 agcctttgcc attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac
     2221 ttcaactaat gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt
     2281 taccatttta gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa
     2341 aaaagctgct tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt
     2401 caaaattatt ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat
     2461 gttgctgtgt tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct
     2521 tcgatgaaag catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg
     2581 tttgaaggga ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg
     2641 attgggatat ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg
     2701 atgtacatta catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga
     2761 cgttcacgtt ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca
     2821 tcttcttcca ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag
     2881 tttgtcaatg ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct
     2941 tcttctttga ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct
     3001 tcttcagcaa cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc
     3061 aagtcaatga tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc
     3121 cagcgacgag tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt
     3181 actgccatgt ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg
     3241 ccggcaaact ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca
     3301 taaagcaccc tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt
     3361 tctgctcaac attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat
     3421 ttctctttgt ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat
     3481 attattgggc aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga
     3541 aagaaaatgc gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg
     3601 atccgtacgc aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag
     3661 gcaacgattt ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc
     3721 gtttcccgct tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag
     3781 gatcggctgg ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg
     3841 acgatcgtca ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc
     3901 aaacctccct ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa
     3961 aatgaggagg acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt
     4021 ttttaatcag agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc
     4081 cttattgaat aaacctttac actctttaca aaaatacatc attttttatt caaatttttc
     4141 aacaccttga ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc
     4201 ttgctctttg tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact
     4261 tctggaccat tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact
     4321 tgggatcaag aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct
     4381 caagtatcgg aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta
     4441 gaatcggtat taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc
     4501 gcatatagac aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat
     4561 gaggtatcgg aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat
     4621 ccgtctatct ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat
     4681 ttggaagaga ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg
     4741 tcaagctttg gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc
     4801 agctcaaaca aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg
     4861 gaactggaaa aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac
     4921 cgcgtgatgg aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg
     4981 gcttttcaac aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca
     5041 cgttatctcg ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct
     5101 ttcattcaag aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga
     5161 tttgatttta atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg
     5221 aaaatcagcg atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat
     5281 attttggaac tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt
     5341 aatttttgat tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt
     5401 tagaaatgct tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat
     5461 gggaaaatta aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca
     5521 atggatagct atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca
     5581 atttgaacat tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt
     5641 ggaaacgatc gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa
     5701 tgctcctatg atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt
     5761 catggcaact tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa
     5821 aaacatgcag tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt
     5881 ctcagatatc caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag
     5941 ttcgctaatg gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt
     6001 ttaaaacagt ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat
     6061 ttaaaagagt taacagaata cattttgtac gctgacatga atccagaatg acttttatac
     6121 aaaataagag gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa
     6181 tagctcctca tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac
     6241 cgtttattcg aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg
     6301 caaacaaaca gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct
     6361 cggtatttgc tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat
     6421 actcggatca gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa
     6481 gtcctagcaa acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc
     6541 gtgcttcttt ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg
     6601 cttgtgctaa tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag
     6661 acaagaccgt ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat
     6721 tcaatgcata gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg
     6781 cacgagcatg tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt
     6841 ccaggttaaa gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc
     6901 caaccccaac ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga
     6961 tttgttcttt agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc
     7021 ctttacgagt tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca
     7081 tgtcgtcaga ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat
     7141 agctttgttc attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt
     7201 acaatgcagc agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca
     7261 acgctggatt caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca
     7321 gcaaatgttt ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt
     7381 caccgttcaa cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca
     7441 tcgattcatc acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac
     7501 gtttgaacca agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga
     7561 attggacgta taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac
     7621 cccatttttt gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt
     7681 tgatgcggat tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa
     7741 ctgtcgaacg caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga
     7801 ttggtactgt tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta
     7861 ctgtttgcaa aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc
     7921 cgacaacttc gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga
     7981 tttgctgtgg cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc
     8041 cgtgttcagc tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat
     8101 ctttgacagc gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc
     8161 ctaaagtgtt cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg
     8221 ccatcactag agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc
     8281 tccatctgat atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt
     8341 tgtatcttct tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt
     8401 actctcttgt tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa
     8461 aacgaaaggc tagtcgtttc ataaaaaaac aatccagatg gcctaaagga gaagaaatac
     8521 cacctggata gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac
     8581 gataatcgtt ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat
     8641 tgccttagcg tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat
     8701 gcttcctgga gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt
     8761 ttctaatttc ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa
     8821 tacgatcagc aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc
     8881 gattacggct tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc
     8941 accaacagag actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt
     9001 aatcgagatg ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt
     9061 gaatgtcaca gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg
     9121 accgactggt ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt
     9181 gactaaagaa accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt
     9241 ttgtaaccag taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc
     9301 cggtgttttc tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc
     9361 atttccgtgg gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc
     9421 tgtttccatt aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt
     9481 gccccctaaa gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct
     9541 accgaatttt ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact
     9601 cgttttttcg tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa
     9661 tgttgctgcc atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg
     9721 agcatatttg ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc
     9781 tttcatttgt tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa
     9841 gatcaaacga tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt
     9901 caacaatttg atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga
     9961 ataaaggacg tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc
    10021 accggatttt tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc
    10081 ttttggtgca acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt
    10141 agccatattg tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa
    10201 gttttcttgg atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa
    10261 gccattccaa actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa
    10321 tccacggaat tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga
    10381 ttctttttta cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc
    10441 gatcgctgca gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc
    10501 aatatttttt ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg
    10561 ttctgaaaaa tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa
    10621 gcttcttcct tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat
    10681 ttaagattaa agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa
    10741 ttacttcaag tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg
    10801 tgacaattca cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt
    10861 cgatacataa tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga
    10921 tccatcattc cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag
    10981 tacaagatca tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt
    11041 aatcttgata tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact
    11101 ttgaattgct ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa
    11161 atgcatcatt gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt
    11221 ttttgcgaat aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat
    11281 cccaacagct actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg
    11341 attttcacga tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa
    11401 aatatattct gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt
    11461 catcccattt cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc
    11521 catgtaaaat ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc
    11581 gatatctttt gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga
    11641 tagaaggaac tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa
    11701 cattttattc aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt
    11761 ttccaagtaa agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc
    11821 ttcttgagca atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg
    11881 tgtcaaattt tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat
    11941 ttcagagaaa acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat
    12001 gtataggata tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca
    12061 ttcctcacat taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat
    12121 ttattcattt tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt
    12181 tttgaataaa ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat
    12241 tgttgttttt tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat
    12301 tttttacata ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat
    12361 gttaaaactt tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga
    12421 ggacttttta atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc
    12481 tgattttgaa catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg
    12541 ttcttataaa aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat
    12601 cttgaccaaa ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta
    12661 tgactatgtg aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta
    12721 cacttataca gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta
    12781 tgaaaaatta gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa
    12841 agtagttgaa atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg
    12901 ggtaaaacaa aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca
    12961 tatcaccatt ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg
    13021 tgaaactgct ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa
    13081 aaagcgtttt actttctgta agacagctac tactccagct atgagagaat aacgcgaaca
    13141 aaaataagca gtgaccctga taaatatcag ggtcactgct tattttcctc cctaaaactt
    13201 ctgaaagatc cggcagtttt agatcactgg acaagactca aagattttct atgtgtgttt
    13261 cagttatgta atattcactg tttgcaaaaa atactgttag attaactaga tgaagcgtct
    13321 tgtcctcatc cgatctagat tattcgatat tcctcgacaa taaacgatta ctcagtgagg
    13381 gcaatcagag gaacggataa tgataaataa gctgtaaaaa atagcaaaat aacaataatg
    13441 attaaaagta tgtaaaaaat agggctttga caaaaacgtg tcacagccct atttcgatta
    13501 aacggtgtgt acctgtcaac tcttcggtca caagtcataa tgttcataaa catgaggagc
    13561 tgttttttga aattgttcct tgtgaatcag agttaaacga caggcttacg aactcttgat
    13621 taaacggcgt tcaaaagctt gcatacgcgg taataagaac agccaggtca aaaacatgag
    13681 aagccgtttt tcctgggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct
    13741 ttattcagat aaaaccaagc ttggatcagc attcaactga tagctagcaa aatttctttt
    13801 ttgcatttcc acatgtgctg ctgctccaat cattgcagca ttatctccac aaagtcgaag
    13861 agggggaatc actagttcta cctcagggag tttagctgcc aaagcatcct gcaatccttc
    13921 acgcaaccct tgattggccg ctacacctcc agctataatc agttgcttga ctggataatt
    13981 ttgacaagct cgaagtgttt tagtaatcaa tacatcgatc acacttgctt gaaagctagc
    14041 tgctaaatca tttttgtcta gatcttctcc acgctgttgt gcattatgca ccaaattgat
    14101 aaatgcactt tttaatccgc taaaactgaa atcataatta tcttcatgga tcatcgctcg
    14161 cggaaaatgg taattatctt ttccctgatg agctagctga tcgatttctt ttccgcttgg
    14221 gtaagaaaga cctaatactc ttccaacttt gtcataagct tctcctgctg cgtcatctct
    14281 tgtctcccca atgatttcat atgatccatc ttcttgcata tagactaatt ctgtatgtcc
    14341 tccgctaaca agaagtgcca tcaatggaaa ctggaatggt ttaaccagac gggctgcata
    14401 gatatgccct gccatatggt ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc
    14461 cttagctgca gaaataccaa tcagcaacga gccgacaagt cctggtccat aagtgacagc
    14521 gactgccgat aaatcttctg ccgacacacc agcttctacc agtgcatctt ccaagcataa
    14581 ggtgatttgt tctacgtgat ggcggctagc tacttcaggt actacgccac caaaacgttt
    14641 atgactgttt atttgagaag caacgatgtt agataagatc tctgttccat ttctaacgac
    14701 cgctacactc gtttcatcac aacttgattc aatcgccaga atcaattctt cattcatttc
    14761 atcgtctccg tttttaactt tctgctcata atgatcgcat cttccgtcgg attgcgataa
    14821 tatgatttcc gtttacctaa tatacgaaac ttttctgatt cataaagttt tcttgctgct
    14881 tcattagata gacgtacttc taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc
    14941 agtaattgta acaaatgccg tgcatgccct tttccttgtt ctgaaactgc aacagcaata
    15001 tttgtgatct cgacttcatc tattactttc gagtatccaa taaagccaca gatctggtta
    15061 tcttcagcta aaagcagata atcagagtga ggctgttgga tgtctgccaa aaactgcatt
    15121 ttcgtccaag gtgaaccatg tggataagca ttttcgctaa tcatccatac gcattctgcc
    15181 aagtacgctt catcaaaatc tttttttctg caaatcaact aatctcactc attttctaaa
    15241 agttttatca tatccaacgc atcttctttt gtttcagtgt aatatctttt tttgatcgtt
    15301 gttgcttgaa accccaattt ccgataaagt ctttgtgcat cttgattact catacgcact
    15361 tctaatgaca tcgtctcaca gcgattcatt atagcaaagt tctcgatttc gtcaataagc
    15421 agcgaaccga ttcgtttacg ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa
    15481 tctaaaccga acgctcttga accgataaaa ccaatcattc ttccattatc aaagatacct
    15541 agatatagat gaggaagcgg tgacttcaat tctgatataa aggcactttt tgtccatggc
    15601 aattcgccat tatagacttc tcgttcgaca tccagcagct ctttgatatc ttctgtagtc
    15661 aattctctta gtacgtaacg acgatcgatt tgttcgactg ttttttctgt ataaatcatt
    15721 ttatcttgaa aaaagcttct ggcatacgct ctaaattttt tcaacataac tttcgtctcc
    15781 aggtgtgtgc gtctccaacc atttttcttc tgcttctact cgttttaaat agcgaggaag
    15841 aaaagcttgt ttctctgcaa ctggttcaac taaagcacct aattgagcca cagttacacc
    15901 acttggaata tcccatgctg gaactgtatt aatcatcgca ctaggcaata tttcctttat
    15961 ttcagagaag aatttgtgac aatcactccc gacgaaataa atagcttcat tcagcttttg
    16021 tagttgtgtg aacaaatcag aagcagaaat atgtacatct tcaagtacag tttctaatct
    16081 attttctttc cagcggtatg ccccagcgta tacatttttt cttcgagcat caaacatggg
    16141 cacaataata ttatccacac cgatgcaatt agccgcgatg gttttcagac tagatactcc
    16201 tactagctct ttatctaatg tataagcaat cgtttttgct gtcgttacac ctatacgaag
    16261 ccctgtataa gaaccgggac catcagaaac cgcgattcgg tcaatgtctt ttggacttat
    16321 ttgaagatct gcaaacaatt ggtcgattgc aggcatcagt gttacactgt ggttgcgttt
    16381 gatatttgtt tgtatttgtc caagaatttg ttcgtcttct accacaccga tcgccaaagt
    16441 ttggtttgca gtatctattc ctaatgtaat catatatttg atcctttctg ctatgacatt
    16501 ctcgccttcc attgtagcat acttttttat tctgaggatg aaaataccaa aggcaaaact
    16561 agtacaataa tacatgatta tctgctggcg ggcaaatttt gccccttcct gaaacacaaa
    16621 aaagctgcgg tatcactcca ttttcccaca gcttttccat cttttatttc gttgattctc
    16681 gaataatcaa ctctgtttcc acaataatct ttcttgaaac cggtgctttg gaattactta
    16741 attgaagaat cgtctccact gctagctctc ccatccattc ggtataaact tttacagtag
    16801 aaagcgaggg cgttacatat ttcgctacac tgacatcatt gaagcccatc acagaaatat
    16861 cttctggtac cactaaacct ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat
    16921 cattagctgc aaaaagcgca tcaggaacat gcggattcgt ttttaaatat tgcttcactg
    16981 cttgatatcc tgactcgaca gaaaaatctg cttcgataat tggaccttcg tcgtagcctt
    17041 tttctgccaa aacttgttga aatgccataa ttctgggatc ttttagccgc tgatggtttt
    17101 tcttcgtata ttctgttcca gcaagcatag cgatatgttg cgctttatgg gagacaaagt
    17161 gttcaatgac tttttgaaca cttgacttaa agtcgacaac tagagagtcg attccttgag
    17221 aagtgccgtc agaatctacc aaaagcaagg cattttggga tcctttcatt tctaataact
    17281 gagcttcgtc gaattttcct agtgcgatcg ttccatctgc cggaagatca ctaatggaat
    17341 cccacgtttc tttcaataaa gtaacgtttg cttcttctgc ctttttttcg attcctaggc
    17401 gaatagataa ataataaaga tcttcaagct cttccgtatc gtcgtaccac tggatcaggc
    17461 gaagcgtttg cttagtattt gctgcatttt tacgatgttt cgtataattt aagcgttctg
    17521 ctgcttcaaa aattttttgc tttgtggcta agccaacgga aagatcagga tcatagttga
    17581 gaactcttga aacagtagca ggagaaactc ctgccaactg cgcaatgtct ttaattgttg
    17641 ccatttgtct ctcctccttt tttacgttta taaaatcgca gtaaatcgtt caaacgctgc
    17701 ctgtcctttt tcatctcgct tgaatactcc agcgtcttcc agtacacgtg caaaaacctg
    17761 acctacagat ttttgtacga catcggtgac cgtatctttc gtcaaatcag gatgttcttt
    17821 ttttaattgt tctgcccacg gacgatgata ttcagccatc tggttttctt ctcctaataa
    17881 gaaatgctct acttctacta gttctggttt caaacgaggt ggtaaaacag caagccccat
    17941 tacttcgatc aacccaatgt tctccttttt gatatgttgg acatctttat gtggatgaaa
    18001 aatcccatca ggatgttctt ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa
    18061 gtaattgtcc cttcttcgtg caattggcgt aatcgtatgg tgtggtgtac catctgctga
    18121 aaaagctctg atagatactg tttcatccga atatgctttc catttttcca atactgagtt
    18181 agctgcttca ataagcaagt ttttctctgt tgattgcaaa cggatcacag acatcggcca
    18241 tttcacgact cctgcgatga cttgcggata atcttctagt ttgataagtt tttctatcgg
    18301 tgcttttgcc ataggaaatt catgacgtcc tgcctgataa tgatcatgag aaagaatcga
    18361 tcctccaaca attggtaagt cagcattcga accaacaaag taatgaggca agacttcagt
    18421 aatccgaagc agtctagcaa atgtttcctt tgtgattttc atcgggcggt gttgttctga
    18481 taaaatgatc gaatgttcat tgtaataagc ataaggggaa tattggaatc cccagctttc
    18541 accgtctaga ttcattcgaa taatccggtg attggtgcgg gctggatagt tcaagcgacc
    18601 tctatatcct tcgttttcca tgcaaagcat gcaggtagga tagtctactt ttaccgcctc
    18661 gcgttctgct gcgatttgtt ttggatcttt ttctggctta gataaattga ttgtgatttc
    18721 cagttcacca tactccgact ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt
    18781 gatgtagtca tttttcttac ttaaattaaa gaaatactca gttgcttcct ctgggctttt
    18841 cgaatagtgc tgtgcaaaga atgcattgac gactgaaggt ggcggtgtga gaaaatccat
    18901 taattgcgct tcaaaaatct cttgttctgc tggcaattct gaaagaacac cattttctct
    18961 tgccttagcg acaagctgat ccaataagtc tacagattct tccttaactg gtcttacttc
    19021 aatgctttct agagcatctt ctcctaccat ccctaaaaga cgattctgta aatataagcg
    19081 gtccatttcc atccatccgc cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg
    19141 actgattgac atatctcatt cccctattct tcataaccat ttggatggct gacatgccaa
    19201 tcccatgctg ttttgataat cgctttgata tctgtgtatt caggtttcca gcccagaatc
    19261 tctctcgctt tttcacttga agcgactaat cggctaggat ctccagctct tcttggtgct
    19321 atttctgctg gaatttcttt tcctgtcact tctctagctg cttctagcat ttccttgact
    19381 gagtatcctt tgctgctgcc taaattgaag aagttgcttt catttccttc tttcaagtat
    19441 tccaaagcta aaatatgtgc agcaatcaaa tcttctactt gaacataatc tctgatacat
    19501 gttccatctg gtgtatcata atcgtctcca taaacagcca aggcttttct ttgaccaaga
    19561 gctacttgta aaatgattgg gacaagatgc gtttctggtg tatgatcttc tccgatagat
    19621 gcatctgctt ttgctccggc tacattgaag tagcgcaaag cgacatagcg catgccatat
    19681 gcttggtcac accacttcat cattttttcc atcatcaatt tactttcacc atacggattt
    19741 ttaggattag ctggtgtatc ttctgtgatt ggagactctt tcggctctcc ataagttgct
    19801 gcagtagaag agaatacgat tttattcacg ttgaactcgt gcatgacttc aagtaagatt
    19861 tgcataccgt atacgttgtt gttgaaatac atcaatggtt tttcgacaga ttctcctact
    19921 aaagagcttg cagcaaaatg gattactcct tcaatcggtt ccttttcaaa tacggaacgt
    19981 agaaattctt tatcccggat gtctccttca taaaaatgag catctggatg tactgcttgt
    20041 ttgtgacctg tgagcaaatt atctaccaca gctacttgat accccttttg aactagctgg
    20101 tctacagcgt gcgaaccgat ataaccggct ccgcctagta ctaaaattgt cataaaaaac
    20161 actcctttga ttagtaaaat tgtgtttgtt gattctatat aatttaaaaa acgaagttta
    20221 cttttatggt gaaaacaaac aaagtaaaat ttcgtaaatt catatggtaa atttataata
    20281 aaacatttag taaacatttt caagggtttt ttgcaaaggt ttacaaacaa acgtaaaact
    20341 agcctttttt tctcttttgt tccataatag atataacaaa taataaatga ggtggattca
    20401 atggaaaaat ttgtatctgg aatattagta ggaagtctgg caacagccgc tgccgttgct
    20461 ggtttggttg cttctgtgaa aaaaacagtg atcgatccaa ttgacgaaaa agaagcaatg
    20521 atcgaagaaa accgtaaaaa agcaatgcgt aaacgcgttt ctcgttaata attatttaaa
    20581 aatacgcatg aagactttct aaaaaacaga taagtcatca tgcgtatttt tatttgtcag
    20641 tcaatataag ttgtgctatt ttatctgcct gtctcagatt ccatggatct gtataaggtc
    20701 tagctactgc tgcagttagc caatccacac cttcaacagg tattccatag caataaaagt
    20761 cagactggat ttcccctgaa gcatcaatca cgcgattcgt acctcgctca acatctatag
    20821 cttctgaatg ataacttcct gtattatctg aaaatgaaaa agggcgaaca agtttgtttt
    20881 ccttcaaatt ttttatgaca ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag
    20941 cttcaatcaa atagcgactg ctgatttctt tatcttgtag agaaataaca tagcggttgt
    21001 gcttttgctg aatatcaaat ggcggttcga tcaaatgaaa aatacccgca tctatcagtg
    21061 ctgctaattc tttcgtccga atgattggcg gaccgatcgt taaaaagcta tttagatgag
    21121 taaaccatcc ccatagtatt tctttgtatt ccttagcaga aaaaacgtgc cattcaatag
    21181 cctgccggat tggatcacgc cagtctttta atgcatcaaa tgttgaagca aatgctcctg
    21241 tgcaattgcc tttttccgct tcttttatct gcccttcaat aaatattcgg ctagcttctg
    21301 aaaagggctc attctgatag gcaagaggag tttctaaata cgaccaatcc catctataag
    21361 ggattagtgc tggatgcttt ccgagacact cctctgaaga gagggagaga aaatcatgct
    21421 caaaattttt ctttggaatc ggcaagtgat attcttcgat gattttcttg taataaaaca
    21481 actcaacgtc ttttttcaga tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat
    21541 gccattgctc caggttttct tttgttagta accgtggcca tgccattgct ccgccttgct
    21601 tttgatttct tcctcgaggg taataaggga gcccccttct tgatccactg tatacaatcg
    21661 gttccttgcc tgatggatga tagaccagtt ttcccttttc gttctcgaag cgccctcctc
    21721 ttgatactgt aaacaatccg acataatcaa aaaaacttaa gcctagacct cttaaaaaga
    21781 cattttcttt aggcggtatc ttcgaaagat ctacatctgc aggattagaa ggaagctgat
    21841 acagtaaaac atttttcttt gcgtagtcta cccacactct ttcttcttct gtctcatcat
    21901 tttcccagtg accgcttgct aaaacaagct gatcgataga atagctttgc tgatctgtct
    21961 gtaaaagata accatctttt cttttgctta ctttttttac taaagaataa acaaactgaa
    22021 tagaagctgg aaattcttct tgcaactttg agaaaaacca gcgttgatag agtccgtata
    22081 aacatcgagt agattgctga tctttttcca gacgattgat ttcttctata attagttgct
    22141 tatttttggt taccgtctgt tcgatataat ctgtagcagg tcctttactc cattgaaaca
    22201 agttgggtcc ctgtgttacc aatcctccac tggacaatgt ttcatctgta aaaagcgtca
    22261 cttgttgaga aacagaattc attaataact ccggtggctg attcaaccgc caaactcgac
    22321 ctccaggacc attgggatcg aaaagaaaaa gttcaagaga atgtctgtcc tgattattat
    22381 ggagaatcct ttctgctaca cttaatcctc taggtccggc accaatgatc cctattttca
    22441 tttgcatgcc tccatttcga taaatttttt caataaaaca gcatggttct gatgttcatt
    22501 ttttgcacca taaacaaaag taatgactgg atgttctttc ataagttgac tgagtttaaa
    22561 aatagcttgc ttcttttctt cttcgaaaat caattcttgc tggtatttct ttgcaaatga
    22621 agcaaatcgt tctggtacat ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc
    22681 tttcaaccac agatctaact gcgctttttc ttttgaaatt cctcgtggcc agaatctatc
    22741 cacgagtatt ctatagccat cctctttttc agcatcaagg taaacacgtt ttaatttgat
    22801 catagttgtt tccttctttc taccttttcg cttagcttat atatgtagat aatagcacaa
    22861 aataaaaaaa atgaacggca caactatcca ttgcgccgtt catgtgatct tggtcaaaat
    22921 ctaatgattg attcggtttt taaagctgtc atacaatcat ctgttttata cttttttact
    22981 tttgattttt ccagaccatc cgtcatatcc acccttaagt aaataaatct cttgataacc
    23041 tgctttacgt aatttatttg ctgtacggat gctcaaactt ttcttttgat cgtaaatata
    23101 gacaggctgg tcttttcgaa tagatcctaa tgaatctttc aaaacagtgt aagggatgtt
    23161 tcttgcacct aaaatatgcc cagcatcaaa cacatctttt tcgcgaacat cgattacttg
    23221 tgcttttctc attccttctc gaaattcttc ttctgtcaaa gtcgttgctg aacgtttggc
    23281 cattactcgt agatagactt cccaaaggat gattgcaagc acaatcaata gcaaaatgat
    23341 attaattacc cataaaacca tgtaatgcct ccgttttttt aagcaaattt caaggctaat
    23401 gaggcagcac caatgacacc agcttcatta ccaagctctg ctaatttgat ttttgttgat
    23461 tctcttactt gcgggaaagt aaattcttta aaatatttct ctacacggct acgtaaaaat
    23521 tctcctgctg ccgatactcc tccacctaac acgatacttg acggatttaa tgtattcccc
    23581 aagtttccgc aagctaaacc taagtagaag catacgcgat caacaaccat caatgcaaac
    23641 ggatcgtctt cttgagccat ttcaaagata tctttacttg ttacttcttc tccgttgtct
    23701 agcattgctt tcagttttga atcccctgcg tattcttctg ccaagaaacg acctaggcgt
    23761 accacacctg tagcgctaga tacagtttct aaacatccgc gtttaccgca agtacattcg
    23821 aacccttcag gatccacagt gatatgtccg atttctcctc cagctccgct cacgccgtga
    23881 atcaaatttc cgccagcaac gatacctccg ccaacacctg ttcctagagt aacgaataca
    23941 acgtctggat cgttttctcc agcacctttc catcgttcac ctaaagcagc aacattcgcg
    24001 tcattatcga tagcgaattt gattcctgtt cctgcctcga tcagtttttt cacaaattga
    24061 acttctgtcc agtttaagtt atacgcacca ataaccgttc cagcttcgct gtccacgctt
    24121 cctggggtac ccatgccgat tccgataaag tcttcggctt tcatattata taaattcaaa
    24181 cgatgattga ttgattcaat gatttcagga acgatatgct taccatcatc caaaatattg
    24241 gtatcgatgc tccatttttg ctgaatctct ccttctggtg ttaaaattgc aaattttgct
    24301 gttgttccac ctaaatcaat cccaataatc ttcttgtcca ttacaaattt cctttctttc
    24361 ggctttcttc aattcggtgc tctcttttta atatgcgcca cgctgtcatg tatgtatcat
    24421 gatcaatcaa acggctatca tacagattac gcaattcgat agtcattaat tcaatgtcat
    24481 agatgcgtgc acccacatag ataaagatgc caaatttttt gaacagctgc tgtacatcat
    24541 ataagctctc catctttaag cacttccttc atattataca agtaaaccat atttttttaa
    24601 acccagtaaa atacatatta caaaaagaaa tacaaaaact agtgtagaaa gaatcctttg
    24661 gtgtatcgaa tagcttgtct gttgtttcgg tagacccaaa acattcccta acactaatcc
    24721 gccaattagc ccaccgacat gtccccaaac atctacagag gtatccagaa gaccaaaaac
    24781 aaatgtcaac acaataaata aaagaaaatg tctgactagc tgctggatcg ctgggttatg
    24841 tttaaaatga aaccctaaga taaacaacgc accgaacaaa ccgaatatcg aagtacttgc
    24901 accaccagac agaacattcg cttcgttaaa agcaaagctt gctgtatttc ccattatccc
    24961 gctgaacata taaatcaaga aaaaacgcca atggccatag attgcctcga tttgctgccc
    25021 cataaaatac agtatcagtg agttcattac gaaatgcatc agtccaaaat ggatgaagat
    25081 tggtgtcact aaacgccacc attcattgaa gtgaacaaga taaggaccat acattccggc
    25141 ataataaggg atctcaagtc gcggaaataa ttccatcatg aggaagaccg ctgtctgtat
    25201 ccctaaaaaa gcataggtcc ataacggtcg tcttaaccat gcacgcaatt ttatttgctg
    25261 ttgataattc atgaaaacct cccatttcat tttagtcaat aaacaattgt tggatcttct
    25321 gatcaaagct ctccggttgc cattgttcat tcaattgttc acgaaaaaca aggctacatg
    25381 tttttttatg aaaattattt aaataccggt cgtaataacc accgccaaaa ccgattcgat
    25441 aaccggctct attgtagata atacctggta caatgaggag atcgatcatc tccggcaaat
    25501 agagcttctc tgattgtggc tcaagaacac caaaactcga acgtacaaga acggactgtt
    25561 catccatttc ataaaacgcc atctgacgtt tcggcaaagt tttaggtaca actattttct
    25621 tttgttcttt ttctgctcgt tggataaccg gctgtgtatc cagttcaaat tcattcgata
    25681 aagtcgtccc tatcactttt gctgacttcc agtactcgct ggcgaatagc tgtttataga
    25741 ttcgctgttc tttttccttt ttcttgccac ttttggccag atttttcagc ttttcgattc
    25801 ctttccggcg taatcttact ttcaattggt ccaaaaactc acccttcctt ctctcataaa
    25861 atggtatagg aaaaaaagaa aaatagcaat cgaactttca aataaatgca taaatagaat
    25921 aaaaagaaga cctagaaatg attggccttc cttttttaat gaatttcaac tgttcagcgc
    25981 gtgtttttgt ctgataaaaa gtgcatacag aaaatatggc gcaccaaaaa gggcaatcaa
    26041 aattcctgta ggtacttccc cacttggtag aatcactcta gcaaccatat ctgcactaag
    26101 cagaaaaatg gaaccgaaca aaccgctcag aacaaaggtc cagttatttt ctttacgtac
    26161 gactagcttc gcagcatgag gagctagtag tcctacaaaa gataaactac ctgtcaccgc
    26221 tacactaccg cttgccaaag caacagctaa caacaaaagg attttttgac ttttagccac
    26281 gttaaggcct aatccttgcg cgcgttcttg accaaatgat agcaatacaa tcctctgtgc
    26341 ataaagtcct cctagcggaa ttaagataac cgcccatgga agcaatgccc aaacataagg
    26401 ccagctcgca ccccagattg atcccgctaa ccatgcattc acaaatccat aattttcctt
    26461 agataattgg atcgtgcata gcaatgttaa tgcagaaagg ccggcattaa cggcaatacc
    26521 ggacaataag attcgattca tacccaaaaa gccatttcga tcataggccg caagatagac
    26581 acagcaagca gctaaaacac cacctagaaa ggctacaact agcaatagaa acggcagcga
    26641 atagtgcgaa aagaaaccta aataaagcat cactaaaaat cctgctccgg cattaatgcc
    26701 caatatacta gaatctgcca attcatttct tgttactcct tgcaagatat aaccagaaac
    26761 gccaagacaa gctcctgcta ataaactgat cagtaatcga ggaactcgaa attctctaac
    26821 aatcagcgcc aaagaagatg attctttccc tatgaacgtg gcaaaaagct gatccaggta
    26881 aataaaaggc gtaccaatca ttaaactacc tatagacagg atgacaagta caaaaagcaa
    26941 gaaatagaac gtccaatata tttttttcac cgttgttccc tccttacttg tatcagtaaa
    27001 aacggaacgc caatcaatgc aatgactaat ccaaaaggtg tttcaaaagg gggattgatc
    27061 atcctcgctg taaaatccgc caacataacc aatccaccgc caactaaaaa agagacaggt
    27121 agaacataac gatagtccgt tccaaccatt ccgcgaacaa ctggcggaac caacagccca
    27181 ataaatgaaa caggtccgac taacgaaacg gcaatggctg ctaacactgc aactagacag
    27241 aaaacaatca accgcgttcg tttaggatga cttccaagag ctaatgcatg tgtttcactc
    27301 aaatgaatca aagatattgc ttttcctaat gaaaaagaaa ccaaaacagc aagtatatat
    27361 aaaggaaaca atactgctaa ttgtttccat gtgatattgg cagctccgcc tacaaaccaa
    27421 aaagcaagat cctgctgcag attaaagaat tgggtaagtg cttggctgat agcagtaaaa
    27481 aaagaactaa tagctacgcc agctaaaatc agctgtaaag gattcatgcc cattttcgaa
    27541 aactgcaaaa aaccaaatac gagcaaagtt acagcaaaag aacctgccaa tgacattaga
    27601 aacacagaaa aatcagaagc tttaggccaa aaagcaaaac taagggccat tgccaacgaa
    27661 gcacccgcgt tgattcctag taagcctgaa tctgctaaag gatttcttgt cactccttgc
    27721 atcaatgcac cgctcaatgc aaaacaactc cctacaagaa atgctcccgc cacacgaggc
    27781 aaacgaatat ttcgtatgat ttggtgactt tgttttgatc tatcaaaatc aaacaacgcc
    27841 tgccaaaaat ctgctttttc cacagtcgct gcgccatttg aaagagcaac aaaaaaaata
    27901 atcaaaagaa caaaacagac caaaagcagg agtctatttt tcttcatagt cctcataatt
    27961 gatcctcctg ctcttttggc aacaaatcat aggttagaaa aactggttta tttgttcttg
    28021 gaagcgtgac aatcgttgca tcaatctcaa atattttttt catacactct tttgtgatca
    28081 cttcttcagg tgtaccttct gtgatcaact taccgttttt cattgctaat aaataatctg
    28141 aaaaacgtga agcatgatta atgtcatgaa tcgacatcaa gatcgttgtt tgtttcattt
    28201 gattgatttc agtcaataac tgtaggatct ctaattggtg tgccggatct aaaaatgttg
    28261 tcggttcatc caagatcaag atatctgttt cctgagcaag cgccatagcg atccagacac
    28321 gctgggtttg tccaccagac agcgttgcga tatcccgcat acgaaatcca gctagacctg
    28381 tagcatccaa cgcccactca attgcttcat gatcttgcgt gtccatgcca ctgaacagtt
    28441 tttgataagg gtatcgtccg taagatacta cttcttctac tgtcatacct gtagggtgtt
    28501 cactagtttg tgctaataat gcaatttttt ttgcaagttc ttttgaactg tatgtagaga
    28561 tatcagagtt attgacaatg atctttcctg atttcacagg tagtatacgg gtaaaacttt
    28621 tcaataaagt agattttcca cttccatttg gcccaatcaa actagtgatt ttcccttcag
    28681 gaatcgtaac agacatgttt tcaatgattt ttttgttttc gtagccggct gcaatgtcag
    28741 ctgctcttaa tctcgtcatt tcacccatcc tttcgctgaa aatcattctc aattatgacg
    28801 ctgtttcaac atttcgtcaa gcgctacttt catatctata gccaaatgaa gcggttgaca
    28861 gcgttcgaaa tatcaggaat aataaccaat gagaaagatt ctcaattatg ttaggaggaa
    28921 agaaatgaaa ttaaaaacaa aagtattcac attatctagc atattgctgc tggctggagt
    28981 actgagcgct tgctccccct cttcagataa agaaacagct acaactgcca attcgacgac
    29041 ttcatccaat agtactcata cagtcacaga tacacttgga cataaagtag agattccaaa
    29101 cgcgcccaaa cggatcatcg gcagttactt agaagactat ctgattgcac taggggaaaa
    29161 acctatcgcc cagtggactg ttggcagtgg ttccattcaa gattatctgc aagatgattt
    29221 aaaggatgtc ccaacgattt cttatgacct tccttatgag aaagtgttaa gctttgagcc
    29281 tgatttgctg ttgatttctt cttccgctac tgtcgaaggc gggaaatacg aacaatatag
    29341 caaaatcgcg ccaacttatg ttgtaaaaaa cggaacagat gtcacatggg aagaacaact
    29401 aaaagatgtc ggcaaagctt tggcaaaaga aaaagaggca gaaaaaatca tttccgatta
    29461 tcaaaaatcc atcaagaaaa ctcgagaaga actagcagat aaaatcgaga acaagacagc
    29521 tgcagtgttg tgggtgacca acaactcagc ctttatggtc agcaaaaatc ggtcaagtgg
    29581 aagaatcgta tacgacgatc tggaatttgg tgttcctgat ctagtggaag aggtctccaa
    29641 aaaagccact tctgactggt ctgctgtttc ctctgaaaag ctagctgaat tagatgctga
    29701 ctacatcatc cttgtgaaca gtgacaaaga tgctgccatg ttcaatgaac caagctggaa
    29761 gaatctaaaa gcagtgacag acaatcatgt actgcaattc ggacctgaat ctagttggct
    29821 atataacgga cctattgcca gtcaggaaat ggtcaatgat atcaaagaaa aactaaattg
    29881 attttaacta aaaaaagcat cttcacgatt agtcgtgaga tgcttttttt cttattaaca
    29941 aactagacgc atagatttta ttcttctttt gaagcaaccc tattttttac tctttcccat
    30001 ttttgaaaac gatgggcata tagattttct tgatccgtta tcccctcata ctcctctata
    30061 agggcgaagt ctgaaaaatc aatattccca atatacgtat cgccttcaaa cgtgctatcg
    30121 atcaatgttc gccagatccg accagtgtaa ggaagaagtt cttgaaaaat ccgactacca
    30181 cccgaaacat agatatcttc tgagatcgtt tgactatacg acaaaagttc atcgatcgaa
    30241 tgcattactt ccgcattttc tttcgtgcga aaatttggtt gcgttgtcaa cacgatgata
    30301 tggcgataag gaagtgaaag atcccccatt ccttcatagg tctttcttcc catcactaaa
    30361 attcgatcca ttgtatgttc gcgaaagaaa cgcatatcat ttggcaatcg ccatggtaac
    30421 aaaccatttt tgccgatcag accattttta tcttgtgccc acatggaaat aaacatatta
    30481 tccgctcctt atgattttag atgttttgag ataaaaaaag tctcaattct ttgaccagca
    30541 aggaattgag aactttaaaa acattatttt gtttcacggt gcaaagtaac tttgcgttcg
    30601 cgtgggcaat atttttgttt ttccaaacga tcaggattgt tacgtttgtt tttgcttgtt
    30661 agatagttac gttctttaca agaagtacac tctaaagtga tgtttacgcg catgtttttc
    30721 cctcccattt ttacaagaat cctctaagat gactctcaga cttgattatc ttaccatgat
    30781 tttttctaga ttgctagtgt ttttcacgat tttgttaagt aaaactactt gacgataaat
    30841 acaacggttt tacttctcaa acaaaatctt tttctttttg tataaatacc cttacacttg
    30901 tttatataga ttatgttttt taataaaggg ggatactaat atgccatgga acatgaagga
    30961 ctttcctgca tcaatgaaaa acttagataa attgacaaga aaaaaagcaa tcgacatcgc
    31021 aaatgcatta ttagatgaag ggtaccctga taaccgtgcg atcccaattg ccattgatca
    31081 agcaaaaaaa tggtatgaag atgctagtga atcagaaaaa cgaactttcg aaaaagaaaa
    31141 aaatccaagc aagacggatg aacacgatac gaatcctcgt gctggaaaac tgcttgattc
    31201 agatgtgatc gtagaatatg aggaagaaca atggatcgtg aaatctaaag gggcaaaaaa
    31261 ggcaagcaac cattttgata caaaaaaaga agcaatcgaa aaaggaaagc aagtagcaca
    31321 aaacaaagaa tcaactttgt tcatttataa aaaagatggg acaaaagaaa aagaaatcga
    31381 ttattgaaca tatgtaaagg ggttgtgacg aaaatcgtgt cacaaccccc ttatgttcga
    31441 aacaatggtg ttcctctgcc aactccgaac cataagtcat aatatctata aaacgggaag
    31501 agctgttttt tgaaattgtt tcttatcact cagtgttaaa cggtaggaga aaacctcttt
    31561 tttattgata tttgattttc atcaaacagt cacatagtcg attgcttctg cttgcggaat
    31621 cgtttcatct acaaagtaga ctttatacca tgtcaagaaa ctgaagattg tccagatttt
    31681 tctttgctct tctttacgtc cttcgtgatg gtcatctagc aacttcatga ttttttcttg
    31741 atcaaaaaat tcttttgcaa aatctgcttc gaataatgct ttgacttgtt cgtagccgtg
    31801 ttcttctttc aaccaagctt tgattggcac agggaatcct aatttcacac gattcgacca
    31861 ttcttccgga agatgtttgt tcgcagcttg tctgaaaata tcttttgtgt tgttttgatt
    31921 caataaaaac ttacttggta tttgttgagc caatttcatg acttcgatat ccaataaagg
    31981 tactcggact tccaaagaac ttgccataga aagtttatct gcctttaaca agatatcttt
    32041 tggcatccat tgatgaagat cgacatattg catcttattg acttcatctt tgattccttc
    32101 tgttttctcg taatgaacag tcatgatttc atccacagat ggtgctgttt gatattttgg
    32161 tgttaacact tctgatgctt ctgattcttc gaacactcga gcatgaccga taaaatattc
    32221 ttttgcagga gcaagtgatt catataaatg gatccgtcca tggaagtttt tcatttttcc
    32281 gattttacgt cctaaattat agcgtgttcc ttttggtaat tttttcaatc cttgagcaat
    32341 cacacggatg aattttgaat tcgtatggaa tccatacgtt tggtatcccg caaacagttc
    32401 atccgcccct tctccagatt ggactacccg cacactttgt gaagcaagat ttgctaaaaa
    32461 gtacaaaggt acgcaagaag gatttgaatc aggttcatct aagtgatact ggatcaaagg
    32521 gaatgctttg aacgacatgt ctccgtcgat cactgctgct gtattatcta agtccaatag
    32581 ttcggttagt tttctggctt caatcgcttc attataggtt ttatcatcaa acccgattga
    32641 gaaagaatga tctggtttca atacagaggt tacataactg gaatcaaccc cgctggataa
    32701 gaaagaacca acttctacat cactgaccgt atgtgcttca atagaagctt gaacggtttc
    32761 gtcgattttt tcgatccatt cttgtcggga atgcgtttct tttgtttcaa aatctgcatc
    32821 ccaatattgc tgaatgtcta tttttccatc tttatacgta taataatggc cttcaggcaa
    32881 acgatagaca tctttgaaga atgtttcgcc gtttaatgga gaatattgga aagtcatata
    32941 aggtttcaac gcttctttgt tcaattcttt gttaaaatca ggatgaggca gaaaactttt
    33001 gatttctgaa ccaaacataa atgttccgtt catttctgca taataatatg gtttgatccc
    33061 aaagtggtct cttgcaccga ataactcatt cttttcatta tcccaaatag caaaagcaaa
    33121 cattccgcgg atcttttgca ataattctgt tccccattct tcgtaaccat gcaaaaggac
    33181 ttctgtatct gcatgtgttt gaaaaacgtg gccagctgca atcaattctt ctctcaaagg
    33241 ctggtaatta tagatttcgc cattaaaaat aatgatcttt gtccgatctt cattgtaaat
    33301 aggctgagta ccgccctcta agtcaataat acttaatctt ctgaatccta aagcaacatg
    33361 tttatcgata tattctcctg aactattagg tccacggtga acgattcgat ccatcattgt
    33421 attaataatt gtttttttgt tgtccttgtc gttaacaaaa cctacaatac cgcacattta
    33481 actttcacat cctaatataa aattactgtt tatttcattc atatgcatct tatcatagaa
    33541 taaatagcgg acgaatattt tctctcttac aatttcgtca cgtgaaaaca aaagatgaaa
    33601 gtttctgttt ttacagaaaa taaagtccca atatctaatt taccatgttt tcttttttga
    33661 ggctacctta ttctcttcgt gttaaaaaaa tattagtgga tgctatttcc aaaaaaacca
    33721 gcaaaaaaag acacgaccta aagatcgtgt c
//
LOCUS       DAYYAR010000018        31634 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000018 DAYYAR010000000
VERSION     DAYYAR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.018"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..903)
                     /locus_tag="ACHAT0_000725"
     CDS             complement(55..903)
                     /locus_tag="ACHAT0_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8450690.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1127..3067)
                     /gene="thrS"
                     /locus_tag="ACHAT0_000726"
     CDS             complement(1127..3067)
                     /gene="thrS"
                     /locus_tag="ACHAT0_000726"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8450691.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3404..3583)
                     /locus_tag="ACHAT0_000727"
     CDS             complement(3404..3583)
                     /locus_tag="ACHAT0_000727"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8450692.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3630..5453)
                     /locus_tag="ACHAT0_000728"
     CDS             complement(3630..5453)
                     /locus_tag="ACHAT0_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8450693.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5731..8010
                     /gene="helD"
                     /locus_tag="ACHAT0_000729"
     CDS             5731..8010
                     /gene="helD"
                     /locus_tag="ACHAT0_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8450694.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8103..8750)
                     /locus_tag="ACHAT0_000730"
     CDS             complement(8103..8750)
                     /locus_tag="ACHAT0_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450695.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8929..9648)
                     /locus_tag="ACHAT0_000731"
     CDS             complement(8929..9648)
                     /locus_tag="ACHAT0_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8450696.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9779..10930)
                     /locus_tag="ACHAT0_000732"
     CDS             complement(9779..10930)
                     /locus_tag="ACHAT0_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8450697.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11147..11887)
                     /locus_tag="ACHAT0_000733"
     CDS             complement(11147..11887)
                     /locus_tag="ACHAT0_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8450698.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11891..12214)
                     /gene="rsfS"
                     /locus_tag="ACHAT0_000734"
     CDS             complement(11891..12214)
                     /gene="rsfS"
                     /locus_tag="ACHAT0_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8450699.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12271..12864)
                     /gene="yqeK"
                     /locus_tag="ACHAT0_000735"
     CDS             complement(12271..12864)
                     /gene="yqeK"
                     /locus_tag="ACHAT0_000735"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8450700.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12848..13498)
                     /locus_tag="ACHAT0_000736"
     CDS             complement(12848..13498)
                     /locus_tag="ACHAT0_000736"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8450701.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13508..13819)
                     /gene="yhbY"
                     /locus_tag="ACHAT0_000737"
     CDS             complement(13508..13819)
                     /gene="yhbY"
                     /locus_tag="ACHAT0_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8450702.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13835..14944)
                     /gene="yqeH"
                     /locus_tag="ACHAT0_000738"
     CDS             complement(13835..14944)
                     /gene="yqeH"
                     /locus_tag="ACHAT0_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8450703.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14941..15468)
                     /locus_tag="ACHAT0_000739"
     CDS             complement(14941..15468)
                     /locus_tag="ACHAT0_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8450704.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15648..16433)
                     /locus_tag="ACHAT0_000740"
     CDS             complement(15648..16433)
                     /locus_tag="ACHAT0_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8450705.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16426..17295)
                     /gene="accD"
                     /locus_tag="ACHAT0_000741"
     CDS             complement(16426..17295)
                     /gene="accD"
                     /locus_tag="ACHAT0_000741"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8450706.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17296..18675)
                     /locus_tag="ACHAT0_000742"
     CDS             complement(17296..18675)
                     /locus_tag="ACHAT0_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8450707.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18683..19102)
                     /gene="fabZ"
                     /locus_tag="ACHAT0_000743"
     CDS             complement(18683..19102)
                     /gene="fabZ"
                     /locus_tag="ACHAT0_000743"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8450708.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19127..19603)
                     /gene="accB"
                     /locus_tag="ACHAT0_000744"
     CDS             complement(19127..19603)
                     /gene="accB"
                     /locus_tag="ACHAT0_000744"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8450709.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19606..20841)
                     /gene="fabF"
                     /locus_tag="ACHAT0_000745"
     CDS             complement(19606..20841)
                     /gene="fabF"
                     /locus_tag="ACHAT0_000745"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8450710.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20860..21597)
                     /gene="fabG"
                     /locus_tag="ACHAT0_000746"
     CDS             complement(20860..21597)
                     /gene="fabG"
                     /locus_tag="ACHAT0_000746"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8450711.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21609..22526)
                     /gene="fabD"
                     /locus_tag="ACHAT0_000747"
     CDS             complement(21609..22526)
                     /gene="fabD"
                     /locus_tag="ACHAT0_000747"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8450712.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22633..22857)
                     /locus_tag="ACHAT0_000748"
     CDS             complement(22633..22857)
                     /locus_tag="ACHAT0_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8450713.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22910..23872)
                     /locus_tag="ACHAT0_000749"
     CDS             complement(22910..23872)
                     /locus_tag="ACHAT0_000749"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8450714.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23882..24322)
                     /locus_tag="ACHAT0_000750"
     CDS             complement(23882..24322)
                     /locus_tag="ACHAT0_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8450715.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24628..25593
                     /locus_tag="ACHAT0_000751"
     CDS             24628..25593
                     /locus_tag="ACHAT0_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8450716.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25650..25880)
                     /locus_tag="ACHAT0_000752"
     CDS             complement(25650..25880)
                     /locus_tag="ACHAT0_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8450717.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25966..26853)
                     /locus_tag="ACHAT0_000753"
     CDS             complement(25966..26853)
                     /locus_tag="ACHAT0_000753"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8450718.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26942..28165)
                     /locus_tag="ACHAT0_000754"
     CDS             complement(26942..28165)
                     /locus_tag="ACHAT0_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8450719.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28182..29228)
                     /locus_tag="ACHAT0_000755"
     CDS             complement(28182..29228)
                     /locus_tag="ACHAT0_000755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8450720.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29392..31119)
                     /gene="ptsP"
                     /locus_tag="ACHAT0_000756"
     CDS             complement(29392..31119)
                     /gene="ptsP"
                     /locus_tag="ACHAT0_000756"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8450721.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31119..31385)
                     /locus_tag="ACHAT0_000757"
     CDS             complement(31119..31385)
                     /locus_tag="ACHAT0_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8450722.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
BASE COUNT         8728 a         6935 c         5442 g        10529 t
ORIGIN      
        1 tggaaaacta cgttttgtta ttctaaaaaa cttatataaa aaatagtaag gtttttattt
       61 cgaatccata aaatattctt ggattgcttc tcgtgttttt ttcacttttt cgctaagctt
      121 ttcgttttta gccatataga aataaaaaat gatatcgatc gtagcaaatt gggccaaaag
      181 tgaattagtt gccgcacttc gattcgtgat ttcttttggt ctagatgttt gtacgagtac
      241 atcggctatc ttgctaagcg ggttatttcc aaattgcgtc aacgcaatga tttcgatatt
      301 catgcttttc gccagctcgg ctaatgcgac tacatctgca cttctacctg agtttgaaac
      361 aagccaaagc actttctttt tctcattgct gacaagttgc gggagcaaga cgtggatatc
      421 tttttcaaaa atgattggct taccgacacg agaccatttt tgcagaatat cttctactac
      481 taaagaagag gcgccaaccc catataaaaa aacagtctct gccgattcca aacactctac
      541 gactttttgg atcgtttctt cttctagaat atctactgtt tcttttatcg ctacttgtgc
      601 attgttacca agtttgtttt tgatagagta aaaggactca tttgcttcta tatcactaaa
      661 accttcatcg attccagtct gtagttctgt tgaaatcgtg attttgaagt ccgtcagact
      721 ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc gcactcaacc cggaagcttc
      781 tgctaattca ttagctgtca ttgtagggac cttctcaata ttaccgataa tgtagttggc
      841 caactttttt tctgctgaag aataatcttc catcatactt tcaatacgac taattacgct
      901 cataagttcc tccacccttt taatgatagt ctatgtaagt ataccatatc agacccttat
      961 tcacggtcgt atactggata aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc
     1021 catcaatccc acagattcta ttgagcatct gtgtctttga ggaatacctg ttatctattg
     1081 ttcggattgc tttctatcta ctactttttt acaggaaatt tacctattag ttttctctgc
     1141 tgtaattttt gacttctgct tccatagcat caataaagat attcaaatct tctacagctg
     1201 tttctttact tccataacga cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa
     1261 caactaattg gtaagggatc ttttgtgttt gagaggcacg tatcttatag cccatttttt
     1321 cattacgatc atcaacttct acacgcaacc ccttcatttg caaacgttct ttgatttcat
     1381 aagcataatc gccatgtgca tcaacagaaa cagggatgat tgttgcttgt ataggtgcca
     1441 accatgttgg aaaagcgcct ttgtatactt ctgttaaata agcgacaaaa cgttccattg
     1501 tagaaacaat tccgcggtgg ataactactg gacgatgcgt attttccccg tcttctccaa
     1561 catatgtcaa atcaaagcgc tctggcaata agaagtctaa ttgtatagtt gataatgttt
     1621 cttctgtacc aagagctgtt ttgacttgaa catctagttt tggtccgtaa aaggctgcct
     1681 cgccttctgc ttcaaagtaa tctagttcta attcatccat agcggctttc agcattgctt
     1741 gggctttttc ccacattgcg tcatcatcaa aatatttatc tgtattgttt ggatcgcgat
     1801 agctcaagcg gaaacgataa tctgtaatat tgaagtcagc atatactgcc accatcaatt
     1861 caagcgtacg tttaaattca tctttgattt gatcaggacg aacaaatgta tgaccatcat
     1921 tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc acctgatttt tcatagcgat
     1981 gcatcattcc taattcagcg atacgaattg gtaattcacg ataagaatgg atcgtgtttt
     2041 tgtagaccat catgtgatga gggcagttca ttgggcgaag aacaagcatt tcgccatctc
     2101 ccatatccat tggcgggaac atgtcttctt gataatgatc ccagtgtcca gatgttttgt
     2161 acaactcaac atctcccatt attggtgtat atacatgttg gtaacctaga ctaacttctt
     2221 tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc cccttttgga agccagaatg
     2281 gcaaaccaga acctacttct ggggaaacca tgaacaagtc aagttctttt cctagtttac
     2341 gatggtctct ttcttttgct tcttcacgga gacggatata ttctttcaaa gctttcttgt
     2401 caaaaaaggc tgtcccgtat acacgttgca tcatttgatt gtttgagttt ccacgccaat
     2461 aagcacctgc cactgataat aatttaaata cttgtatacg gccagtggat ggaacatgtg
     2521 gtccacgaca caaatcgatg aattcgcctt gttgataagc agtaatcact tcttcttctg
     2581 gcaattcact aatcagttct actttgtacg gatcacttgc aaaaatttct aatgcttcct
     2641 gttttgaaag aacacggcgt tcgatcggca gattttcttt gacgattttc atcatttctg
     2701 cttcgattgc cggcaaatct tctgcagtta cttggttttg tccattatct gtatcgtagt
     2761 aaaaccctga atcgatagct ggtccaacgc caaagtggat atttggatag aggcgacgca
     2821 tggcttgggc catcaaatga gctgctgaat gacggactaa gccaagtgca tcttcatggt
     2881 ctggtgtgat gatctcaata cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt
     2941 ttccgttcac tttaccagct aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa
     3001 ttgccaatgt tgttgttcca gactcaaatt ctttgactgc accatctgga aaagtaattt
     3061 ttatcataat agtttcctcc ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac
     3121 aaaaaaatcc tagcatctct tgaaaagaga tactaggacg aaaagttcgt ggttccacct
     3181 aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa
     3241 caagggtttc gaaagtggtc atcctttggt tttctcctgg aaaagtttca gcctagcttt
     3301 tcctctctta tcgaagactc tcaaattcag ttggctttct catcaatcaa ttatgtgttt
     3361 atttaaccac taataaatag aaaaatcaag tgggaaacta aacttatttt ttccattccc
     3421 catttacacc gtatgttcct tttttcatct cattgatgat gacatggatg tgttcttttg
     3481 gtgcctgcgt gtttttatga acagcttccg tgatatcttt catcatattt tctaattgtt
     3541 ccggactgcg accttctact aattcgacat gtacgaatgg cataatgctc tctcctcttt
     3601 ctgatcgttc ttttttcaac tcgattctat catactatgt ttgtcttggg tactgaatca
     3661 ggaaaaatgg ctggatcttt ttttgctctc aaggcactca aaatagagac cgtatccacg
     3721 acttcttgca aaacagctcc gaataatgcg gggattagtc ccgtacttgc aattaacatc
     3781 aaaaaaatac aaataaagat gccaatcagt acagactgtt tcgctattct catcgtatct
     3841 cttgaaatca ctactgcttt tgctacacgc aagagatcat ctttcaagat gaccgcatct
     3901 gcactttcac tagcagctgt agaaccatga gcccccattg caataccgat atctgcagca
     3961 gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg caacaggtct ttgatcttta
     4021 gggatacttt ctaaaatggt cagtttatct tgaggcaagc tacgggcatg tacagaatct
     4081 aattggactt tttctgcaat cgtttgagca atggcagatc catccccggt gatcatcatc
     4141 aaatttttca catcaagttt tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc
     4201 tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa ttgttacata gattgctgta
     4261 ttggacgttt ctatttcttc agctgaaaca aaagaggcac gtccaatctt tacttcatga
     4321 ttattgacta ctgcttggat tccttgaccg gtcacttctt tcagttgact aacaggatac
     4381 agttggctgt ttttcacgtt gttcactaac gaacgagcta agatatggct agattcctgt
     4441 tctgcactag ctgcatacag aagtagctct tcttttttta ttccattgac tggctgaatc
     4501 gcctctactt ccaatatccc tttggtaagc gttcctgttt tatcaaaagc aatcgttttt
     4561 gtacgtgcga gcttttcaat cgttgttcca gtttttacaa cgatcccgtt tttacttgaa
     4621 cggctcattc ctgcaactaa agcaatgggc gcagctagaa tcagtggaca aggtgatgcc
     4681 actaccagta cctcagcaaa tcgtaaaggg tcttttgaga accaccaaga aataccaccg
     4741 atcaaataag caatcaacgt gaaagggact gcataacgat cagctaaacg gacaaatcgg
     4801 gcaggctttt cttttgattc ttcgactagt ttaataatca tttgatactg gctgtctgcg
     4861 gcacgttttt cagcgatcat ttggatcgat ccgtctccat ttaaagaccc tgacataatc
     4921 tgatctccag gtttcttggt ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc
     4981 gaagattctc ctttggtcac tcgaccatct gcgggtacaa gttcgccagg cttgactagt
     5041 aactggtcat caatatttat ttcatcgatt actatatcct gcaattttcc ttctttgaat
     5101 cgatgggcaa ttcttggggt gttatctaac aaagactgca gttctttacc cgctcgttta
     5161 ttcgcgtaat cctctagact atctccgccc gtaagcatga tcaatatcat caaactagcc
     5221 cagtattctt tgacggctaa tgttgcaagg attgcggtaa tcgctaatac atctacgcca
     5281 tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca tcaagaaggc gagaatccct
     5341 ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat
     5401 aaagctatcc caccaataag caaagtaagt aaaaattttt tgaaatgtgt cattgttttt
     5461 tctcctcttt tatgtgactt agagaggtta gtaataaatt tccagctgcc tctttcatct
     5521 taagcatagt ccttgactag cactttttaa aacaataggc cttaattgaa aacaactatc
     5581 actatcagcg tttccaaaat tcatcagttc tacaaacaaa aatattcagc aaggattact
     5641 ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa ctatgctata cttttaattc
     5701 gttatttacg gcggaaggag caaacatgcg atgaatgaga aacaactaga acaaacgcac
     5761 gtggatgaaa cgatcgaact aatccagatc gaacaaaata tgctggatac aaaacaacaa
     5821 gaattgactc aggaaatgaa tcattcgaca aaagacacag ctaatcacaa gattcgagct
     5881 ggttccaatg aagcttttta tgaatctgct gttgaatatc gccaacatga gcaggaattg
     5941 cttttgaaat accatactct tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg
     6001 aaagatagtc cttattttgc ccgtatcgac tttaaagaag accgtgaaac agaaacatta
     6061 tatctaggta tcgcttcttt aagagatctg gcagaagaaa caatcgttat tgactggcgc
     6121 gcaccaattg ccaatcttta ttatgaaggt gagattggtc ccgcatttta tgaaactgaa
     6181 acagatagaa tagatgtcga tctgcagttg aaacgacaat ttaaaatcca agaaggacgt
     6241 attttatcta tggtagatac gtcggaagta atcaatgatg actttttgct agagatattg
     6301 gatgaatcat caagcaacca tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat
     6361 gcaatcattc gggacacaac aagtaaagta atgctgattg aaggaatcgc cggaagtgga
     6421 aagacatccg ctttgcttca acgtattgct tttctgttgt atcataatcg caagtggctc
     6481 gaggctgaaa acgttcttct attttcaccc aaccatttgt tttctgatta catctcgact
     6541 gtattgcctt cattaggtga aagcggcgtg cctacacaga cattcaaaaa ttatttggtg
     6601 cagctgctgc cagagttttc tttactagat gaacagcaac aagaaagtgg ttttttgcta
     6661 ggggaaaaag atccgataca agtgatgaaa agcggattga ccttagtcga tcagattgat
     6721 cgctatatcc aaggcatcac aaactacggt cccttgttcc gtgatatgaa aataaatggc
     6781 cgtacgatcc tatcaaagga atcgattcgc caatggtata aagaaacaaa tgaacaatta
     6841 cctttacatc atagactttc tctattacaa acgaagctcc tgaaaaaatt aggcggtctg
     6901 caaaaagacg aaacacgtca gcaatgggtc aaagatctgg cggaagaaca gctccaagaa
     6961 ctatacgcaa cagatccaaa tcttgaatat acagaacaaa aggagcgttc attacgtaaa
     7021 aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat
     7081 tatcaatttg tcaatataac gaaacaatat ctccactttc tacaaactgt gccactttct
     7141 cttctaacac gacataaaat cagcgagacc aggtggcagg aacatttgac aactgttcgt
     7201 acacacttgc gtcaaagaga attgcgtcaa gaggatgctg tactattctt tttactgatg
     7261 cgccgaacat atccagtgac ggttacacac aaagctcgct atatctttat tgatgaaatg
     7321 caggatttcg caccatcaca agtggcattg ctgcaagcct tgttcccaaa agcaaatttg
     7381 actttctgtg gagatctaaa tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg
     7441 gaagaccttt tcgtggaccg tgaagtcact cgtttccaat taacgacaag ctatcgctcc
     7501 acaaaagaaa tcactgattt tgctaatcat tttattgtga aagaacatca agtagaaaca
     7561 acagcaagaa aaggagacct tcctttgctc atccaaggaa caacagatga tatcaaattg
     7621 aattggttag cagataccat tcgaacgact cagggaaaag cccggtattg gcgtacagca
     7681 atcatagcta aaacacaaga agaatgcgaa gctctttatg ctcaactgcc agacgagttg
     7741 aaacaacaag tccagctgat tttaaatgag agtgacttta tgaagcgttc cattatttta
     7801 cttccagcct ttttagcaaa aggtctggag tttgaccgag ttttcttgtg ggatgtagat
     7861 gcagaacatt tttcttctca gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg
     7921 atgcatgaat tgaccctttt tacctccaat aatgaaagtc ctttgttaaa caatatggat
     7981 atttcatctt atgagaaaaa agtactgtaa gtaaaaagac aactgctatc attaatgtta
     8041 gcagttgtct ttttatttac aataataatt gcagaaaaag taattcaatc atttaaggtc
     8101 atttattcta tatataaatc caaatctgtt tcaggatatt ttaatttgac aagcgaaata
     8161 atcattttca gttcttccat gttttctgga tattctgggt caaatacctt gctatgacgg
     8221 ccaatcccgc cattgtcgat caatcggtaa attcgattcc attcaatgaa ttggtcaatt
     8281 gattggaaat tgctgattgg atagccgcca caatccaaaa tctctgatac acgaaatgct
     8341 aggtatgcac tatcgatcac tttatttttt ttcgtgtcta tcttctcatt tctttcgata
     8401 agaatttttt caggacgatg tttcaagctc cactctaata gtagaaagaa agcctccatc
     8461 cgatactctt cttcaaataa ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc
     8521 agataatttt gtctataaat cagcattggt gcgcggtcca ccatacactc taaataacgg
     8581 ttcagatacc ctgtaccgac atagtttcct aatcgatagc cttcaacgcc gaatcttgcg
     8641 tatttttgcg ctaattttga acaatcagtt acgttcacag ctaactcttc tactttgcgg
     8701 taaccgctta agatacgttc aaaaggttct aaagtgattt ttgcttccat attcttcccc
     8761 gtccatcttc cacatacttc atatcatttc acatgatact tatgctctca tcataccaca
     8821 ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt tagtgagatt
     8881 gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa
     8941 tgccacatta tggaacacat gctgtacatc ttctagatct tccagtgcat caatcatttt
     9001 ttcaaatgtt tcttgatctt ctggagatag tgttacctcg ttttgaggga tcatttggat
     9061 ctcagcgatt gaaaaatcag tgattccttt ttcttttaat gcttcttgta tactatggaa
     9121 atccgctact tcaccgtaaa cgatgatttg tccttcttct tccattacat cgcgaacatc
     9181 gatgtctttt tccattaggt attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa
     9241 gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt tcccgccatt
     9301 tttcccaaat gctgcccgta cgtctgctgc tgttcgattc acattgttcg tcaatgtatc
     9361 aacaatcacc attgagccgc ttggtccaaa tccttcatag cgcaattcag agtatgtttc
     9421 atctcctgca ccttttgctt tttcgattgc tcggtcaata atatgttttg gtacattata
     9481 agttttagcg cgatcaatga cgaaacgtag tttttggttt gcatgcggat caggatcacc
     9541 tgattttgct gccgcgtaga tttcaatccc aaattttgca taaatccggc tattattagc
     9601 atctttagca gttttctttg ctacgatatt tgcccattta cgtcccatat tttcacttcg
     9661 ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt tccattatac ttgtgaatca
     9721 cgattctgtg aagctttttt tctttcttcc ttcattcttc tcatttttag tcaattatct
     9781 atactcggat tgggatttgc ccgaaattct gttccggaat attgcctccc aattgataga
     9841 tttgatcact tctaactaac agttccgctt cttgtgcctc tttttttccg attcgggaat
     9901 aaatagaaat ctcttttttt tcttttaaga atcttcttcc agcatcggta aatccaagta
     9961 gtcgaagata atttttattt tgttggacag gaatctcttg ctttttgata ttcagcaaga
    10021 cataacaaga taatcgttgg atccgtgtcc atgtatatct ttttgttttt acctttgaca
    10081 caaaatcctc aaaactatca gcagttgcag ctgctgtttt catgcgcgat tctattcctt
    10141 cggtcatctg atagatatct gtcagttcca gcaaagtaga agataacaac cgataacgca
    10201 aatacggcca ataagattcc caagtctgca gcgaacaagt tgaaagatct tcttctgtct
    10261 gaggcggcac aagtccagct attctttttc cttcttctaa cgcatggcga atggctgttg
    10321 cactggctat cctctgatct gtgatgattt tttcatgata gcctgcactt ttacgtcgga
    10381 tcggatgtaa ttccataggg tggacatact tggcattttc ctttgcataa cttaatccca
    10441 agatatgatt tggcgaagaa aaatcaagcg taatatcggg atacagactg cgaaaaacct
    10501 cagtcatttt ttgcggataa ctatacgttt gatcagacag ttcttgaaat gcctcttcta
    10561 tcctatcttg attctcttgg acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt
    10621 cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg caagattttt actgcacctt
    10681 tagcaaaata gtcagctgac tgaaccgccc attcgacggg aagctccact actaaatcca
    10741 caccattttg taaagcttca ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc
    10801 tttgcaaaaa attcccgctc attacagcaa tgacgacctc tgctcctgtt atttttcgtg
    10861 ccatttccac atgatagcga tgtccattgt gaaaaggatt gtactctacg ataatcccgc
    10921 agcttttcat gcgcatcctc cttcatagtt atcgataaaa aagaaataga gacaagtaaa
    10981 aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac tattctcgca aacacaatac
    11041 ttctgcgacg aaaagctgca ctgcaagagc aatgcctata tcgttcggat atataattta
    11101 tgttgaagat ttattctttt cttgttaact cgagcaggca aaaccgctag tctttgtatg
    11161 ctacaaagaa ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg
    11221 cttcgacttt cgaaaagccg ctgttttcca gcattcttaa ataactttcc aatggatagg
    11281 tacgctcttg atgtagttca tcttcacgaa taaatcgacc agacccttct tgttcttcaa
    11341 cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct tcctggataa ctatcccaca
    11401 aaaaagcaaa attatcccct tgataatgat agctatattc tggaaaaacc tcatctactt
    11461 gataggtgga gtgtacgtca aataaaaatc ggccattttc ttctaacgct tgatagactt
    11521 catcaaacac ttgctgtacc tcttgtctat ttttcatata gcacagcgaa tcagaaaaac
    11581 aggtaattgc ctgatattgt ccgatttctg ataaatcgag catattgcct tccacaaatt
    11641 ggatcgatgc gtcttcttca tatgctcgct ggctggcaat catcaacatc tcttctgaaa
    11701 ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc aaccgctaat gctccagtac
    11761 cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta
    11821 accattgctg ataaagtgtt tcgtccatca cttcatcgta aatgaaagca aatgtttcat
    11881 aagccatatg ttaatccaac cattcactaa gatcgactag cggagcatct gaccatagtt
    11941 tttccagatt atagaaattt cgttctggcg catggaaaac atgtacgatc acatctccaa
    12001 gatcgatcaa tacccatttt ccgccttctt ttccttcaat acgttttact tcatatttgt
    12061 tttcttcttc tttatcaatg atttcttctg taattgcgtt gatttgacgt tcgctatttg
    12121 ctgaacagat catgaaataa tctgccaaca aggaaacatt tcgaacgtct aaagcgataa
    12181 tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat ttttaattgt tctttactat
    12241 ctatgatgat ttcctcctaa tatgagcagc ttattttttt gctacccagt aattatatgt
    12301 ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt
    12361 tgtttcaaat gctacagctt cgtctagatc agcccatgca atggctcgtg catcttgtac
    12421 cccaggaaaa tctcgacctg gttcaatata gtctgctaca taaatgattt tgtcaaggag
    12481 actcatctta gcagccccag tcgtatgcac ccggattgca tgaagaatct ctgcatcggt
    12541 gattcctaat tcccgctcta caaacgaagc gccaacaaga ccatgccaga ttgcattatt
    12601 ataattcaat aactccggat caaaaccgtc tcttacgatt accattttga actcctcatc
    12661 tggtcgttct tttgcataat catgtgtcaa tgcagcaata ctagcttttt caggagaagc
    12721 accgtatttt ttggcaagag ccacggctgt ttcttctact ccaagcacat gcttgaagcg
    12781 ttgttcgctc attctcatct gtattttttg catcaggttt tctctagaat aagcagtata
    12841 ttcattacta tagtccatat tcatacaacc cttcattttg aatataatca attactttat
    12901 ctggaacaag gtagcggata gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac
    12961 tgatgtcgat ctcaggtaca tctacccaaa ttacaggata tggagtatcc gttgtgtatc
    13021 ctggtcgacg tatacccaca aagttgacca tagaagtcag ctcatcgatt ttataccatt
    13081 ttggcaaata ttctaccata tccccaccga taataaagta ataatctgta tccggattgt
    13141 tttgcgtcaa ttccttcata gtatcatagg tgtaactttt cccaccacga gctaattcaa
    13201 tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat
    13261 gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa
    13321 atacgcggtc aagacctaaa ttccggccag cttgttcagc catcactaaa tgtgcaaggt
    13381 gaacgggatt gaatgttccg cccaagatcc ctacttgctt tttcttctct aagaattgag
    13441 gcatttcttc aggaaataat tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg
    13501 tcactcctta tattgctttt acttcttttg atagcctttg atacttttct tttgtggaag
    13561 gtttgtataa tacaataaca cgtccaatga tttggacgat ttcgcaatct atttcttttt
    13621 ctaatacttg agctgcttct tctgcaatct catctgtatt ttgaagtaac gatactttga
    13681 tcagttctct tttttctaaa gcttcttcaa tttgaacgat cattgctgaa ttgatcccac
    13741 cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt
    13801 gttttcctct taaactcata attttccttc ttccttaaat caatgctttg cgtttcaata
    13861 catctacacc ctttggcgcc cagcccgcaa ttactactgg cgctgttaca gtgatccagc
    13921 ctagaccggc aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa cgaacaagct
    13981 ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa taatccacct acatgttttt
    14041 gataaaattc gtctgctttt tccagtttcg ttcgatgaat atttacatca tttgacacat
    14101 aagcaataaa tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga
    14161 aaagtgtttg cccttcattc agctgataaa ctttaggttt gatttctttt aacggtgcaa
    14221 tgatccgcaa atctttttta cctaagtagt gcgccatttg atggcgatgg ataattcccg
    14281 gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc gattttatcc aaggtcgttc
    14341 ctggaaactg cgaagttgtg attacatctt gtactcccgc tgtatttttg atgatttgat
    14401 tgataagcgt cgattttccg acatttgtta ctccgacgat atagacatcc cgtccatcgc
    14461 ggtattcttc gattttttct aatagttccg tcatctcatg agatttctta gcactagtta
    14521 ataatacatc tactggacga attcctgctt cgtaagcacg ttccttcatc cattgtgtta
    14581 atttagtttt tttcaatgat ttaggtaata tatccacttt gttcccaact aaaagtactg
    14641 gattgtcacc gacaaaacgg tgtagtcctg ggattagtga accattgaag tcaaaaatat
    14701 ccaccacatt gacgatcaat gcatcttttt gccctaattc attcagtaag cgcaaaaagt
    14761 cgtcatccgt caatgagaca tcttgggtct cattataatg gcgtaatctg aaacaacgct
    14821 gacaatagac ttccccggaa gctaaacctt tttccaaagc cgtttgcggc gtgtatccta
    14881 attcaccggg tttgtttgtt tgtataacgg caccacagcc gatacattgt aattcttctg
    14941 tcattcgatt ccacctcgcc agcccatatc agggtgtttt gctaatagat aggccataat
    15001 tcttttttcc caaaaacgat taaattgtgt cttccaacta tctgttgcga taatgggttt
    15061 tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt atatcagtca taatctgatc
    15121 gcctaccatt actatctcat catctgctaa atccaattgt ttctttgcta tgttgatccc
    15181 tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg
    15241 cgcgacacgt tttgaattat tgtttgaaac aacaacaaca gggattcctg catttttcat
    15301 ttctaaaatc caagtaagca actcttctgt tccatctgga ttattccatg caatcaacgt
    15361 attgtctaaa tcggtcaata ctgctttgat gcctagattc tttaattggg caggtgtaat
    15421 ctgatagatg gcttctacca tccatgttgg tttgtaattt gaaaacatgc tgtctcctca
    15481 tctgcaaaaa atgaaggagc acataaagta ctcctcaacc ccgtattata ctgcatatca
    15541 gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa aagcatttct ttacaaaatg
    15601 accaaaaata aagattgatc acagaaaaat ttagaaacaa aaaacaacta atattggcgg
    15661 aacctcttgt atcgtttttc cagtaattta tgctgatcta atcgaacaag ttccgtcaat
    15721 ctttcaatca acgctttttt cataatgtgt gcgactttat atcgtggcaa ctgctcgccg
    15781 ttgaacgttt caggaataac tcgatcaacg attcctaact cgcgtagttc tggcgcagta
    15841 attttcatca actcagctgc ttctgcggcg cgactaccgt ctttccacaa aatcgacgca
    15901 aatccttctg gagaaagcac agcataaatc gtttcttcca gcatccagac ttcatccgct
    15961 aaacctagag caagggcacc gccacttcca ccttcaccaa tgatgatggc gatgatcggt
    16021 acctttaaat cagacatttc gagcagattt ctggcaatag cctctccttc gccgcgctct
    16081 tctgctccta caccgcaata tgcacctgct gtattgatca aggtaatcac cggtctattg
    16141 aacttttccg cttgtttcat cagtcgtaaa gcttttcgat agccttcagg atggggagaa
    16201 ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt
    16261 accggtttgc catctaatcg agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa
    16321 cgatcaccat ggcattcctc gaaagaatcg aagatctctt caaaaaaatc caagcttgtc
    16381 catcgttctt tagctcttgc taactgaacg acatcatgtg ctgtcttatc cacgatctat
    16441 ccatcctttc tgtgtatgca attcaagaag cgtatggatt ttctgtttca attccatgcg
    16501 gggaacaatc tgatcgacaa aaccatgaga taataagaat tcagcctttt ggaaatcttc
    16561 tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct
    16621 ttggggttct gctagaataa tatcgccttc cattgcaaaa ctggcagtta ctcctccggt
    16681 cgtcgggtcc gttaaaacag ttagataaaa aagaccttca ttgctatgcc gtttgacagc
    16741 agcagagatt tttgccatct gcattagaga aaagattcct tcttgcatcc gtgcgccacc
    16801 tgaagctgtg aacaagacga ctggaagact ttcctttgtt gctcgttcga ataatctggt
    16861 gatcttttct ccgacaatcg ttcccatact tcccatgata aaattaggat ccatcactcc
    16921 aataatgaag ggaatcccct gaatggttgc tttaccagtg aggactgctt catgaagtcc
    16981 tgttttatct tgcatttttt taattttttc cgtatattcg ggaaatgcta gcggatcttt
    17041 ggttacaaga tccgtatccc attcttcaaa acttttttca tcaataatca aagctagacg
    17101 ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg catacttttt cttcaccgat
    17161 gtctttcgta taaaggatat gtttgcaatt tggacacttt gcccacatgt tatcaggaac
    17221 agatggcgag ttagcaggtt tgctctctct attaggattg atccgaatat atttcttttt
    17281 cttaaacaga gccatctatg cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa
    17341 ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg actgcaggat gactaatcaa
    17401 atccatctga aattctgcgt tcgtgatgat tccttctgtg ataaattcac ttaaggctcg
    17461 ctgcatcttc attaatgctt caaaacgcgt atttccatgg acgattattt ttgcaatcat
    17521 tgaatcataa tatggcggga tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc
    17581 catcccacct gctggaagca gtaagttttg gatcttccct ggagaaggag caaaatggaa
    17641 agcaggattt tctgcattaa tccggcattc gattgcgtgt ccttggaata ctacttcttc
    17701 ttgacacaca ttcaaaggct caccggctgc tatctccacc tgttttttta ctaaatcgat
    17761 tcctgtgacc atttctgtta ctggatgttc cacttggatt cgagtattca tttccatgaa
    17821 ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt ccggcatttt cataattgac
    17881 tgctttagct gctcgaacag cagcgtctcc tagtgcttct ctttttgttt gtgagatcac
    17941 tacagaagga gattcttcca gtactttttg attgttgcgt tgtaaagaac aatctcgctc
    18001 gcctaaatga atgacatgac caaatcgatc acctaagatc tgaacttcta tatggcgagc
    18061 aggataaatg attttttcca aatacatatc atcatttcca aatgccgctg ctgcttcttg
    18121 ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat tgaactttcc gaatgccttt
    18181 cccgccacca cctgcagctg ctttcagcat cactggataa ccaattctgt ctgctatttc
    18241 caatgcttct tctactgtag ataatacacc agtacttcct ggaatgactg ggacatttgc
    18301 ttcttgcatc aattgacgcg cattgatctt atttcccatt gcatcgatcg ttgcactttt
    18361 aggaccaata aatgtgatat tgcactcttc acacatggag gcaaatcggc tattttcaga
    18421 taaaaaacca aatcctggat gaattgcttc ggcttttgtc acgattgcag cgctcagtac
    18481 ttcctgaaca ttcagatatg aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc
    18541 tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa tatatcgcta cggtttggat
    18601 ccctagttcg cggcatgcac gaatgatacg tacagcgatt tcaccgcggt tagctatcaa
    18661 tactttagaa aacatatttc acctatccaa tcataaaggt taattccgct tcggctactt
    18721 ttttaccgtc tactttagcg attcctttgc ctattccggc tgaagctttc actttcaata
    18781 tctcaacttc aaggtataag gtatctcccg gtgtaacttt cttacggaat tttgctttgt
    18841 ccaatccgcc gaaataagcc gtctttccct tgaattgttc aagagaaagt aatgcaactg
    18901 ctcctgcttg agccattgct tcaacaatca atacccctgg cataacaggt tcttctggaa
    18961 aatgtccttg aaaaaagggc tcattgatcg ttacattttt cttagcaacg atccgttctc
    19021 cttcaaccat ttcttccact cgatcaatca aaagcattgg ataacgatgt ggaatgattt
    19081 cttttatttc ttgtatgttc attgttttcc ctctttcctg ctttgcttat ttttgctgaa
    19141 tcctaaagag tggctgtcca tattcgacaa ctgactcgtt ttctacaagg atttctgtga
    19201 tcactccgtc ggcctcacta gttatctcat tcatcagttt catcgcttca ataatgcaga
    19261 taacttctcc tttggcaact tgatcaccta cttttttgaa agctggtttg tcgggcgctg
    19321 gttgcagata aacgattccc acgattggcg aaacaacctc gattccatca aatactgccg
    19381 attctttttc tttcattatt tctggagcag ttgttgcttg ttcaggtatg acattgactg
    19441 gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt catgcctcga cttgttttat
    19501 ttttattcat atacaattca aattgacctt cacgcaaatc gaattccgtt agcgaggatt
    19561 gatcaaattg actgactaat tctctgattt cattgatatc caaaattatt cctcccagcg
    19621 tttcatgcac agaaccgcat tatgtccgcc aaaccctaat gaattactca ttgcataagt
    19681 aaaacttgca ggtttgcttt cgtttttgac aactgtcaag tcaatctcag gatcttgttt
    19741 ttctacattg atcgtaggtg ggataaattg atgttctaat gccagcaaag ttgccaaggc
    19801 ttcgatcccg ccggctgctc ccaataaatg tccagtcatc gatttcgtgc tgctgacgta
    19861 agtatctctg aaattttcac ctaatgcgat ttggattgct tttgcttctg cctggtcatt
    19921 ggctggtgta cttgttccgt gagcattgat atatccgact tgttccggct gtacaccagc
    19981 ttcttccatc gcaagcttca ttgctttaga tgcaccagaa ccatctggag taggagctgt
    20041 catatggtaa gcgtcacagt ttgcaccgta tccaacgatt tctccataga ttttggcacc
    20101 gcgagcttgt gcatgttcta aagattcaag aactagaacg cctgctcctt cacccatcac
    20161 aaaaccattc cgattttcgt caaacggaat cgaaccacga ttaggatcag ttgattctga
    20221 gagtgctgtt aaagctgcaa atccagcaat ccccatctca caaatcgttg cttctgttcc
    20281 tccagcaaga atgacatcag aatagccatg tttgatgtta cggaatgctt ccccaattga
    20341 attcgttccg ctagcacaag ctgttacgat agctgtacaa ataccttttg cacctacacg
    20401 caaagcaata tttccagctg ccatgttggc aatcgccatt ggtacgaaca ttggagaaac
    20461 tcgttttgca cctttttcat gtagacgaat aacttggttt tcgattgttg gaagaccacc
    20521 gatcccagat ccgatgatga cgccaaaccg gtcttgattc attttttctt tatctaaacc
    20581 gctcatctcc attgcttcta aagcagcgta gattccatag atcgaaaaca tatccattcg
    20641 tttgctatct tttttaataa aatatttatc gtaagggaaa tctttgactt cacccgctaa
    20701 ggtaatccct gtttctgaag catcaaattt agtgatcggt gcgatgccat tcgtcccctc
    20761 ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata ggtgaagtga ctccatagcc
    20821 agtaattacc actcgattca tttgtttgct ctccttttct tatccgtgca tgaccaatcc
    20881 gccatcgaca ttgatgactt gtccggtgat atatggattt tgtgctaaga aaactgctgt
    20941 ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc
    21001 cttgactttg tctgctaaaa cttccgtcat atcagttgtg atgaatcctg gcgcaatggc
    21061 attacatgta atgcctctag ttgcagcttc tcgtgccaca gattttgtta atcctactac
    21121 gccagcttta ctagctgcat aatttgcttg accaatattt ccaatcaacc cagatacact
    21181 agagagatta atgatggccc cttctcgctg cttcatcatt tttttcaata cgtgctgcgt
    21241 catattgaag gttccaatca aattgatatc cagacatttt ttgaaatctt ctgcatccat
    21301 tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt actaacacat ggatcgatcc
    21361 aagcttttct tctgcttctt tgatcatttg tcccgctttc tcataatcag agatgtcacc
    21421 ggaaactcct acgcatttta caccgaatgc ttcgatttct tctatctttt cttttggaat
    21481 ctctccgcga ccattcaaaa tgatatttgc tccagctttt gcaaaagcta aggccatcgc
    21541 tttgccgatt cctcttgtac tacctgtgat aaatacattt tttcctttta catccataac
    21601 gttcctcctt atagtattgc tattgtttca tcaaatgtct gcttattctc aacacgcaga
    21661 actggtattg ttttatcgat ttttttcata aatccgctta atacttttcc ggggccgact
    21721 tcgatgactt gttcgatgcc gattgttttt aacttatgga tactctcata gaaacgaaca
    21781 ggttgcatga cttgctgttc aagtagtccc gcgattgttt ctttcttcat tatttccgtt
    21841 gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt gaatctgact taggtccttc
    21901 gccagttttt ttgctgccgg ttctaaaata gctgtatgaa aaggcccgct gacatttaga
    21961 gggatcattc ttttgaaccc tttttctttt aatagtaaaa cagcttcgtc tactgctttt
    22021 tcttcgccac caatcacgat ctgctgaggt gtattataat ttgcaggaga tacgatgccg
    22081 tacttgctag cttcatgaca gctttcctcg atcgtttcaa tcggcgcatt catcactgca
    22141 accattttcc cgcttccagc tggggcagct tctgtcatat aagcgcctct ttttgctact
    22201 aacgcaactg cttcagtgaa ggatagcgca ttactagcta ctaatgccga atattctcct
    22261 aaactcagtc ctaaagctgc atctgggatg attccatgct ccttcaataa gcgataatac
    22321 gcgatgctga ctgttagaat agctggctgt gtatattgcg tctgatccaa acgttcattt
    22381 tctgtaaagc aaagttcagc catatcgtag ccgagaatct cacttgcttc atcaaatgtc
    22441 tgctttacaa cggcttcttc atataaatct tttcccatac cttgatattg tgcaccttgg
    22501 ccactaaata agaatgctgt tttcatgttt ttctccaata tatccagaat atatgtttga
    22561 ttcttcctat tcaaaaaggc cgagacattc ctatttcagg aaatgtccca gcccccaacc
    22621 taattttaat gattatgcag tttgtttttc tacgtaagtc actaaatctt gaaccgtttt
    22681 gattccgtct tctgtttcga ttttcacgtc aaattcatct tcaatttcat tgatgatttg
    22741 gaataaatcc aagctgtcag cttctaagtc ttcttggatg tcagtagtca atttgatttc
    22801 ttcagtttct tttcctaatt cttctgcgat gatttcttga attttttcga ataccataat
    22861 aatatattcc tccgatattg tttgcttttt atttttataa tttaacagtc tacaactcca
    22921 tgagaatact tccccatgtt aaacctccgc catagcctgt aaatattact ttctgctgtt
    22981 ttcctaaggt gatctttcca ctagaaactg cttcatccaa taagataggg atgcttgcag
    23041 cagatgtatt cccatattta tccatattgg tcaggaactt ttcttgcggc atctttactt
    23101 ttcgggcaat tttctcaata attctgaggt ttgcctgatg caaaagcaga taatcaactg
    23161 gtttgtcttt catgacttct tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa
    23221 tatctctgcc agtcatttgc aaataatacg aaccttctga atcttctgaa tagaacggat
    23281 ttttattttg ctggtagcca gcggtcaatg agctacttct ggtaccgtca gctgctaatt
    23341 tttctttcag caattttttc tgtggattcg cttctaagag aatcccacct gctccatcgc
    23401 caaacaacac agcagtcgta cgatcagacc aatcgattag cttagatagt gtttcaccac
    23461 caatcaccag tcccttagtt gagccgcagc ggatcatttt ttcagccatg cttaacgcat
    23521 aaacaaatcc tgagcacgca gcactgatat caaaagcgat agcatgagtt gctcccaatt
    23581 tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc gggcgacatt gttgctacta
    23641 aaataaaatc caaatcttta gggtttagat ttttctgttt tatcaatttt tccgccacta
    23701 aaatgcataa atcagaagtc tcctgatttg ttacacatct tcgttcacga atacccgtcc
    23761 ttgacgaaat ccattcatca gaggtatcca tcatcttgga aagatcatta ttagtaataa
    23821 tcttttcagg aaggacgctg gctgttgcag taatcattcc aaattgttcc attaaatatc
    23881 ctcacttata gtcttgcaag aagtcgtgca agtttttcag tgcatccagc aaggcatgct
    23941 gttcctccgt agccattccg tctaagatgt tcttgaccat ttcacgatga aaatgctgat
    24001 ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa ctttacaacg cggcggtcat
    24061 cttcactgcg gatacgttcg acatagcctt tttttactaa cttgttaata gcagtggtca
    24121 aggtcccaac agtaattgat agttctttgg ctacttctga agttgttttt ttcttgtaca
    24181 tcccgattgc ctcgatcgtg tgcatctctg tgattgacag atctttgaat tgagattttt
    24241 tcagctcaga ctcttcgatt gtcaaaatat cattgaagac actaacaaga taatcattta
    24301 ctgtttttaa atctggttcc atgttccacc gtccactaat tagtttgatt atcaaatctt
    24361 ttgatgttca aactatatct taactttttt caaaatgcaa ccttaaaaga tcattttttt
    24421 actatatgag ctttttttaa gattttactc atttaaaaca actgataaat cagcatttat
    24481 aatttctttt attagaaaaa tcatctatct cacatatttt gattatcaaa gtattttctg
    24541 tgaacagaac tatagaattc cctaaaactt gtccattatt tcaaaatttg ttatagtctt
    24601 tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa aaatgaaaac tctccccatg
    24661 ctgccgggtg tctatcttat gaaagaccga gatggtaagg tcatctatgt tggtaaagca
    24721 aaacggctca aaaatagagt aagcagctat tttcatcaga ataaacagca ttcaaaaaaa
    24781 gtattacgta tgattcatca tataacagat tttgatttcg tcgtagtaga cacggaatta
    24841 gatgctttat tgctcgagtg tcaattgatc caacattacc gtccttttta taaccgacaa
    24901 atgaattatt tcagtaatta taattatgta catattacca ataagggatt tgtattaaca
    24961 gatactccta cggctcgaac atacgggcct tttcgtctct ataaaaaaat gccgtctatt
    25021 ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt ctgaaattag tttgttagct
    25081 ttgcgagtcc agcttcctga cctacaggaa atgacttttg aacagaaaaa aaaggaactc
    25141 caagggctgt ttcaaggccg aaataaaaag ctactgactt acttgaaaaa aagacaacaa
    25201 catttcatct atcagttgaa tttcgaaaaa gccggtatgc ttcaaaaaga tatcgaatta
    25261 gttacttatt ttattagacg gatccaggaa caaaagcagt ttttaagaac accatcgctc
    25321 accttcagca tgccattagc agcagatgag agtcagaaaa agcactacct cgtttgttat
    25381 ggtcaacttg cagaaacaat gattacctct ggtgacgttt ctcctgactt ctattatgaa
    25441 aagaaagaag ctcatttatc tttgaaacgt caattgtcaa aagaagaaat cgatcctgta
    25501 caaatcttga tcagttaccg caaaaaatta gaaaaagaac agtttgaaat tgaactgtta
    25561 aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc tcggcttctt ttaggcatct
    25621 tcaacttcaa atgtcaatta tcctaaaatt tattggatta agtcatagat ttccatcgct
    25681 acgatatcga tattatcaaa ttcgtattgc tgatgagtat ctgtttcagt taattcaaat
    25741 gtatcggtcg ctttatcata agttactgta cacttctcta cgccttcttt ttcaaaacgg
    25801 cgaacttgca gttcattatc tgctgactcg accatagcct ccagtcgttt tatgattgcg
    25861 actaattgtg aatgtgtcat cggaggtcct ccttttctta tttctgttat attgtatcaa
    25921 aagcatagcc tacttgcatt gattttctct aaaaaaactc gttttttatg attttttttc
    25981 tggtattttt ccttcaggcc accaaagttt gatcaaagct tgggttccgt cattctcaaa
    26041 acctttatct gccaactctt catatagttt caatgcttgg gtagttgctg gcaatacaag
    26101 atccattttt tctgcttctt ctaatgcaat ctttaaatct ttgataaaat gtttgacaaa
    26161 aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa taattgctta aagaccagtt
    26221 agctgcactt ccacctgaaa gcgtctcaat cactttcttt aaatcaagtc cgtttttttg
    26281 tgcatagaca agcatttccg tcattcctgt catcgtacct gcaatcatga tctgattagc
    26341 catttttgta tgctgacctt tcccagcaga accatgcaat gtaaatgtcg taccaaattc
    26401 tttgaataaa ggcagtactt gatcataaac ggcttcttct ccaccgacca tgatcgtcag
    26461 tgtaccattc tttgctccta agtcaccacc ggataccggt gcatctaacg catctgcgcc
    26521 tttttctttt gctgtttttg caattttttc tgccaggctt ggtgtacttg tagtcaaatc
    26581 aactaaaata tgtcctgaga tatctgcaga aaaaatcccc gactgtccat agtagatttc
    26641 ttctacatct ttggggtaac ctaccattgt aaagagaata ttactttgtt ctgctaccgc
    26701 ttttggtgta tctgcccaga tagctccttc tgttactaaa ggatcagctt ttgcttttgt
    26761 ccgattatag acagctactt cgtgtcctgc ttctagtaga tggcgtgcga cagcacttcc
    26821 catgacgcct gttccgataa aaccaagttt cattttttcg acctcctaaa aatcaaaaat
    26881 ccttgcttct atgctagtat acgctagaaa caaggatttt ttcataaaaa atgtttgaat
    26941 tttatctttt aaatgaattt aattttattt tgattcgttc aatcgagtct tcttcttctt
    27001 ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg ataaaattcg atcatagctt
    27061 cgccgaaatg tgtagatgaa atatcataaa gtttagcttc taaaactttt ggatctttag
    27121 gaacttcact ggaaatataa tcaataagtg ctggtgcaaa gtcttcgtcg gtttcaaaag
    27181 ttttccctaa tgaaggatga tcgaacaatc ggttcaaata agcatttcct tccacgacac
    27241 aaggtgttcc ggctgccatc gcttctgtat aagtaagccc ttgtgtttct gaagtagaag
    27301 cactgacaaa ataatctgcc gctttataat aaagtgctac atcattgttg tccacttctc
    27361 ctgtgaactg cacaaattct tctaactgca gttctgagat caattcctct aactcttcta
    27421 cataaggtcc ttttcctacg ataactaatc tggcgttagg ataagcggag atgacctgag
    27481 gaaacccatt aatcaaggct tggatatttt tctcataaga aatcctgctt aaagaaagaa
    27541 tcatcaaatg gttttcctga agacctagtg actcccttaa tttgctgata tcctgctgag
    27601 tgatatcaga acgtttgaat ttttcaatgt cgataccagt aggaatcacg cgaagcggtg
    27661 ctattacacc atagctgcgt aatgtatcaa taacacgttc acttggacaa actacacctg
    27721 tcgtatgatt tgcaaataat ctagacaagt attttacatg ggattgcctg atcacttttc
    27781 ctttaccgat ataatgaagg tagtcttcat acatcgtatg gtaagtatgg atcagaggta
    27841 ttttcaactt cttagctacc atcttcccaa gtatccctgc tccgaattct gtatgggtat
    27901 gaataagatc tagttctagt tctttggcaa ccaaataagc ataccacatg cctctaacaa
    27961 caactcttcg atccttgaaa gaaataaatg gtacacttgg cattcgaatc acatcttttt
    28021 ccaacttatc cgcattagga tcggtcgttg taaaaatata gacttcatgc ccatgtcttt
    28081 ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc gctgacttgc ggaaagtagg
    28141 tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa actagtcctt gacctgattt
    28201 acttttttta atttgctaga gactttttca ttcagtacac tttcgtatac atttttaagt
    28261 tcttcaccga ttttattgat acttcgctct tttgctgttt gataaccggc ttgtgtcgta
    28321 ctcggtagtt cttgattggt aatctgttgg atcagtcgga taaattcttc gtttgtcttc
    28381 cccatatagc agttcttgct atcttctaac cagccattat acactggaat atctcgaaca
    28441 agtacgtttt gtttgcttgc taaagcttct aaaacaacga tcccttctgt ctcttcataa
    28501 gaagggaaaa agaatagatc tgcacctgaa tacgcacctt ctatgatctc tccacgaatg
    28561 taaccaggaa acaggacatt ttcaggatga tcttctttta caattttccg gatgttccga
    28621 ggaatcgagt acatgggaac atgaccaaac caaataaatg tgtattcggg catcttctta
    28681 gcaatttcta cgaaatcaat gattccttta cgttcgaaaa acaaaccgac acaaatgatt
    28741 actttttgat ccggagacag tttgaaatat tctctgaact tttgttcttt cattggatca
    28801 gggtgatatt tctctaagtc gatcccattg gaaatagctt gaatcggcac acggatacca
    28861 tagctttcca acaactgttt tgaataaggt gttggcgtga tcagatagtc cgctttttgg
    28921 tacaatccta ccagatattt ttttacgata ggagaaagct ggttcgatct gataaaggaa
    28981 tttcggaaat cttcctctgt agaatgcgca tgatagacaa ctttttttcc ttctcttctg
    29041 gctttattga ccatattatg actattgatt ccgtatgtat tgatgtgcag gatgtcataa
    29101 tcttcttttt catctagtgt ataagagata ccgacttccg tcagagcacg tttttgatga
    29161 tcaagcgcac gcccgattcc agattttgca agcatctttt cactttcaaa atataataat
    29221 accttcacct tacgtccccc ttcacttata tagtataaca tagcctacaa actgtcactc
    29281 aaacttaagc cctatttttg tctattctta ctgacaaaaa cgttttctta aaaaagaaca
    29341 aaaaaactgg cgtagctctt ttacaagcca caccagcttt tgatacgttg tttattttac
    29401 atattcatga acaagttcaa cgacttcttc catcatatcg cattctttca atgcacgatc
    29461 agcaagttca gccattttcg ctgtgtccaa gcgtttcatt aaactacgcg ttttaagaat
    29521 agatgtcgca ctcattgaga actcatctag acccatacct actagcaatg gaacagctgt
    29581 ttgatctcca gccatttctc cacacatacc tgcccatttt ccttcagcgt gtgctgcatc
    29641 aattacattt ttgatcaaac gtaaaattga tgggttatat ggttggtaca agtaagaaac
    29701 gcgttcgttc atacggtctg ctgccattgt gtattgaatc aaatcgttcg tacctacaga
    29761 gaagaaatct acttcttttg caaacttgtc tgctaagact gctgctgctg gaatctcgat
    29821 catgatgccg acttggatcg tatcagatac ttccttgcct tcacttatca atttttgttt
    29881 ttcttcttca aagatcgctt ttgctgcgcg gaattctttg agtgtagcta ccatagggaa
    29941 catgatacgt aaattaccat gaacagatgc acgaagcaat gcacgcattt gtgtacggaa
    30001 catttcgtcg ccttgttcag ataaactgat acgcaatgca cgatatccta agaatggatt
    30061 catttcatga ggtaattgca gatatggtag ttctttatct ccaccgatgt ccattgtacg
    30121 aacaactaca ggttttcctt ccataccttc taatacagct ttgtaagctt catattggtc
    30181 atcttctgtt gggaagtctg gagaatccat atacaggaat tcagtacggt acagaccaac
    30241 tgcttctcca ccattattat ggacaccaac tagatctttt ggtgttccga tgtttgcagc
    30301 taattcaaaa tgcttgccgt cagctgtgac agtttctgca ttttttaatt tttcccattc
    30361 tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat tcagcttttt gttcatctgt
    30421 aggatcgatg attacatcgc cttcgatccc gttgacagcc aaaatatcgc cttctttgac
    30481 tttagcagta atttcttttg tcccaacgat tgctggaatt tctaaagaac gtgccatgat
    30541 agcagaatgt gaagtacgtc caccaatgtc tgttacaaac gcttttacaa aattgcgatc
    30601 taattgtgct gtatcacttg gagtcaagtc atgtgcaaca acgaccactt cttcgttgat
    30661 cattgatgga tttggtaaag taacgcctaa taagtgcgct aaaatacgtt tagcaacgtc
    30721 acgaatatct gcagcacgct cttgcatata agcattatct tccattgctt cgaacatacc
    30781 gatatacata tccgttactt cttttagcgc tgattcagca tttacgctgt tgtctttaat
    30841 attttgcttg atttgaccta ccatttctgg atctgaaaga accatcaaat gggcatcaaa
    30901 tacttgcgct tctgcttcac ctaagctttg cgctgctttt tcacgaattt gctgcaattc
    30961 ttcagtagat ttagctaatg cagcatccaa acgagcttct tcagccgcag tatcttctac
    31021 tgttgcctta ctgaatgtta aatccggttg aactagcagg taagcttttg caacggctac
    31081 accgtcacta gcggcaatcc cttttagcat ttcaaccatt attcagataa gccttctttc
    31141 ttcattgttt caacgattgc tgccattgca tcagcttcgt cagcaccttc tgcagtaata
    31201 gtaacatcag aaccttgacc tacacctaaa gacataacgc ccatgattga tttcaagttt
    31261 actgatttac ctttatactc taagtttaca tcagagttaa atttgcttgc tgtttgtact
    31321 aatagtgtag ctggacgagc gtggatccca gtttctgcta ctacgtgaaa ttctttcttt
    31381 tccatattaa tcggtctcct ttaagtgagt gttgttttta tgtgagggtt ttctttttta
    31441 gtaattcaga gaatcctaac aaatgccacc ctttcatctg taaagattaa catttttttt
    31501 cgttagatac aacagttttc attcaatttc agataaaagt attcaaatta ttccggattc
    31561 tttttcatcc ttttcttcac attttataac aaacgtgttt atttcctttt tcttgtcgct
    31621 ttctcttctc ctga
//
LOCUS       DAYYAR010000019        30202 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000019 DAYYAR010000000
VERSION     DAYYAR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.019"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACHAT0_000758"
     CDS             complement(38..1189)
                     /locus_tag="ACHAT0_000758"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8450723.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACHAT0_000759"
     CDS             complement(1202..1639)
                     /locus_tag="ACHAT0_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8450724.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACHAT0_000760"
     CDS             complement(1654..3420)
                     /locus_tag="ACHAT0_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8450725.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACHAT0_000761"
     CDS             3684..4361
                     /locus_tag="ACHAT0_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8450726.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACHAT0_000762"
     CDS             4364..5491
                     /locus_tag="ACHAT0_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8450727.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACHAT0_000763"
     CDS             complement(5691..6131)
                     /locus_tag="ACHAT0_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8450728.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACHAT0_000764"
     CDS             complement(6142..8175)
                     /locus_tag="ACHAT0_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8450729.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACHAT0_000765"
     CDS             complement(8177..9880)
                     /locus_tag="ACHAT0_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8450730.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHAT0_000766"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHAT0_000766"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8450731.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACHAT0_000767"
     CDS             complement(10963..11988)
                     /locus_tag="ACHAT0_000767"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8450732.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACHAT0_000768"
     CDS             complement(12197..13066)
                     /locus_tag="ACHAT0_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8450733.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACHAT0_000769"
     CDS             complement(13525..15417)
                     /locus_tag="ACHAT0_000769"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450734.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACHAT0_000770"
     CDS             complement(15414..17141)
                     /locus_tag="ACHAT0_000770"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450735.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACHAT0_000771"
     CDS             complement(17157..17807)
                     /locus_tag="ACHAT0_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8450736.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACHAT0_000772"
     CDS             18011..18307
                     /locus_tag="ACHAT0_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8450737.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACHAT0_000773"
     CDS             18479..19099
                     /locus_tag="ACHAT0_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8450738.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACHAT0_000774"
     CDS             complement(19191..20357)
                     /locus_tag="ACHAT0_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8450739.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACHAT0_000775"
     CDS             complement(20369..20722)
                     /locus_tag="ACHAT0_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8450740.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACHAT0_000776"
     CDS             20850..21872
                     /locus_tag="ACHAT0_000776"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8450741.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACHAT0_000777"
     CDS             21891..22052
                     /locus_tag="ACHAT0_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450742.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACHAT0_000778"
     CDS             complement(22311..22745)
                     /locus_tag="ACHAT0_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450743.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACHAT0_000779"
     CDS             complement(22855..24714)
                     /locus_tag="ACHAT0_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8450744.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACHAT0_000780"
     CDS             24715..24855
                     /locus_tag="ACHAT0_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8450745.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACHAT0_000781"
     CDS             complement(24832..25992)
                     /locus_tag="ACHAT0_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8450746.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27210)
                     /locus_tag="ACHAT0_000782"
                     /pseudo
     CDS             complement(26299..27210)
                     /locus_tag="ACHAT0_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(27376..28569)
                     /locus_tag="ACHAT0_000783"
     CDS             complement(27376..28569)
                     /locus_tag="ACHAT0_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8450747.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(28653..28853)
                     /locus_tag="ACHAT0_000784"
     CDS             complement(28653..28853)
                     /locus_tag="ACHAT0_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8450748.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(29277..29510)
                     /locus_tag="ACHAT0_000785"
     CDS             complement(29277..29510)
                     /locus_tag="ACHAT0_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8450749.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(29517..29948)
                     /locus_tag="ACHAT0_000786"
     CDS             complement(29517..29948)
                     /locus_tag="ACHAT0_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8450750.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         8479 a         6440 c         5483 g         9800 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaattata tgggatttta gattatcttt ttagaagttc
    27241 ttgaatgtcg gtatagcccg tgtttccggg cttttccggc atttttgata ttgttagata
    27301 cttacaaatg ttcttattac ctctcaccat ttccccctca aaagtagtaa atggagtagt
    27361 aaaaccggcc ttgtgtcagg ctgccagccg ttccatttct gcccttgcgg aatcgaatgt
    27421 ggcgtgtgca taatagttca gcgtcatggt gatattggaa tgtcccataa tgtactgcaa
    27481 ggctttgggg ttcattcccg cattggctaa ccgggtgcag aatgtatgac gcatagcatg
    27541 gggcgtcgtc gttttcggca acgcttcttc atggctcttg ttgtacttct tcacaagcct
    27601 gccaaacatc ccgccataat ctaccgccgt catgggatag ccgttctgtt tgagaaacag
    27661 gaaattggca tacccgtcaa taatgaacgg ctttgcacct ttgcggtttt ttatcactcg
    27721 ctggaacgcc tgatacactt cctcattcat ggggattttt ctgacgccgc tttgggtctt
    27781 gggttcgtca atatagtaac cgacgccggt atttctcaaa agctggtggc tcacgatgat
    27841 tacccggttt tcaaaatcca aatctttgtc cgtcagtccg cacagttccg aaatgcgaag
    27901 ccctgtcccc agcagaataa cgattgcgtc ataatgcttt tggtaaacct tatcaccttg
    27961 aataaaggac agaaaacttt cttcctgtgc tggtgtaaga ggcacctttg gttccgtatc
    28021 gtcgtccaga acgtcactaa gctggaagtc aaacgggttt tttcggatgc aatcgtcctg
    28081 tatggcggtg taaaaagccg ctttcaggga acgcttgtcg ttattgatgg tcttataaga
    28141 aaggcccttt tctttcatcc gaagcgccca ttcctttgcg tcggacagct tcacactgtc
    28201 aatcgggcag ctccccagcc gatcttgttc cagaatacgc atcagccgtt cccggcccag
    28261 tttggtgttg tgcttcacat tgccccggtg cctgatctgc ttggcgtaga gctggcagac
    28321 ggtcattttc ttgccgatgg tgtctatccc gtcgtcaagg tctttctgga tttctttctc
    28381 cttttcccgg agtgaaatat cctcccgttt acctgccggg gtcttatccg taggcactaa
    28441 cttccatgag taaacaaact gcggttttcc aaaggtatca acatatttgt aagcatatcg
    28501 tccgtctttt ctctggctct ctccagaccg caaaacgcga tttttgctgt ctcgtctttt
    28561 ttctgacatc tcgtcatgct cctttccatg acggaaagag ccgcatatat gcttataaat
    28621 tattataaca tatctgcggc cctttttcac tattagattg tgtccaaagt gtcaattatt
    28681 ttctcaaact gcttgcgttt gatctggatg cggttgccat tcataatgac ccagccagag
    28741 gccggatttt cctcggccag cttacgcaat ttgttttctc cgatacggaa atatttagac
    28801 gcttcctcaa tcgtcagcgt gtatttttcc caaataggca catcatggtt cataatgcag
    28861 ccccctttca caaattcaaa ggtttgtgtg atttaggggc atagacggac ggctgctggc
    28921 acagctccac gggactttca cccccgcgtg gttctcacgc ggccctaccc attgcctgcg
    28981 acgcttttaa cgctcggact gtggctataa gggagtatca tagtcctgcc tgtacgtcgt
    29041 cgcccgcaag ccgccacgct cacattgcgg cgtggtgttc ctcgctccgc ttttcccgta
    29101 gggaaaagta tcatggcgca ccagccgtcc ggctcggcac agacagaatg tgtccgtatg
    29161 ccctatgatg cgccgccgtt atcttgcggg catatttatc aaggtgcgat caggccaccg
    29221 ggaaaacacc gaaacgggga aagggtaagc cgtttcatgc tgccggggcc ggttcgtcag
    29281 ttcgtgacga tagcgtggat gagcttgatt tccagacggc gcttcatggc ctcgtccaga
    29341 cacacatgcg gatagccgtt ttcgtcatag agcgtccggg tacacagctt gttcatgtaa
    29401 ccgctgtaat actgcaaaac gcgctctacg gctccggcat ccccagcgcg ggcggcctcg
    29461 atcaccgaaa ggggcaaaag ctcaatgccc ttgtagttcg ggttcttcat ccgcgtctaa
    29521 cctcccttcg gcatcaacgc ctttagctgt ttccgcagct ccgataaggt atttgtcctg
    29581 tgccgctgaa cggcactccg ggacattccc acaaggccgc cgatctcacc atctgccata
    29641 tccagaacac agtgcaggat taaaatactc tgctccatgc tgggcagcgc ggcaaaggct
    29701 tcggcaacaa gctcattgtt gatatgtagg tcatagccat gcgacgaaaa cgtaaagctg
    29761 tcgctggggt agtgatctac tgtacagagc ttgtccaaat ctgcctgtga caaagagctt
    29821 agtgacactt cacggtcacg gcggcgcttc atttcagcca agtagctttt ggcctcgtta
    29881 cgcagtaccg tcttgcaaaa agcatcgaac atgcaccgcg cttcatagtc gcgggggata
    29941 gcatccatct tctcaccccc tttctgtggg ggacttaggt ggttctctcc ctttccgcta
    30001 acaagacttt gacaaggccg ccagctaccc gctaaatacc tcctttttga aaatttctca
    30061 aaaaattttt ttggggacag ccgggccgca aaagacgaca aaaatcgccc ggcaggtcgc
    30121 aaacctaccg ggcgagaaaa tgctttgcta tgctgctgat ttacatggta tagtacatac
    30181 tcataaccgg aatagccctg tg
//
LOCUS       DAYYAR010000020        29395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000020 DAYYAR010000000
VERSION     DAYYAR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.020"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..250)
                     /locus_tag="ACHAT0_000787"
     CDS             complement(<1..250)
                     /locus_tag="ACHAT0_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFM8450751.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEI"
     gene            complement(341..793)
                     /locus_tag="ACHAT0_000788"
     CDS             complement(341..793)
                     /locus_tag="ACHAT0_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8450752.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(806..1216)
                     /locus_tag="ACHAT0_000789"
     CDS             complement(806..1216)
                     /locus_tag="ACHAT0_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8450753.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1491..1703
                     /locus_tag="ACHAT0_000790"
     CDS             1491..1703
                     /locus_tag="ACHAT0_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8450754.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1706..1918
                     /locus_tag="ACHAT0_000791"
     CDS             1706..1918
                     /locus_tag="ACHAT0_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450755.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2058..2213
                     /locus_tag="ACHAT0_000792"
     CDS             2058..2213
                     /locus_tag="ACHAT0_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450756.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2210..2434)
                     /locus_tag="ACHAT0_000793"
     CDS             complement(2210..2434)
                     /locus_tag="ACHAT0_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8450757.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2512..2700
                     /locus_tag="ACHAT0_000794"
     CDS             2512..2700
                     /locus_tag="ACHAT0_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450758.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2839..2967
                     /locus_tag="ACHAT0_000795"
     CDS             2839..2967
                     /locus_tag="ACHAT0_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450759.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            3026..3361
                     /locus_tag="ACHAT0_000796"
     CDS             3026..3361
                     /locus_tag="ACHAT0_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450760.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3594..4535
                     /locus_tag="ACHAT0_000797"
     CDS             3594..4535
                     /locus_tag="ACHAT0_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM8450761.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4537..5427
                     /locus_tag="ACHAT0_000798"
     CDS             4537..5427
                     /locus_tag="ACHAT0_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8450762.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5470..6270
                     /locus_tag="ACHAT0_000799"
     CDS             5470..6270
                     /locus_tag="ACHAT0_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8450763.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6285..7136
                     /locus_tag="ACHAT0_000800"
     CDS             6285..7136
                     /locus_tag="ACHAT0_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8450764.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7202..7336
                     /locus_tag="ACHAT0_000801"
     CDS             7202..7336
                     /locus_tag="ACHAT0_000801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450765.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            7337..7798
                     /locus_tag="ACHAT0_000802"
     CDS             7337..7798
                     /locus_tag="ACHAT0_000802"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8450766.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7824..8276
                     /locus_tag="ACHAT0_000803"
     CDS             7824..8276
                     /locus_tag="ACHAT0_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450767.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8382..8576
                     /locus_tag="ACHAT0_000804"
     CDS             8382..8576
                     /locus_tag="ACHAT0_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450768.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8607..8900
                     /locus_tag="ACHAT0_000805"
     CDS             8607..8900
                     /locus_tag="ACHAT0_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450769.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8831..9370
                     /locus_tag="ACHAT0_000806"
     CDS             8831..9370
                     /locus_tag="ACHAT0_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8450770.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9445..9912
                     /locus_tag="ACHAT0_000807"
     CDS             9445..9912
                     /locus_tag="ACHAT0_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFM8450771.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10631..10798
                     /locus_tag="ACHAT0_000808"
     CDS             10631..10798
                     /locus_tag="ACHAT0_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450772.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            10824..11168
                     /locus_tag="ACHAT0_000809"
     CDS             10824..11168
                     /locus_tag="ACHAT0_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8450773.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            11173..11454
                     /locus_tag="ACHAT0_000810"
     CDS             11173..11454
                     /locus_tag="ACHAT0_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450774.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVDIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            11557..11871
                     /locus_tag="ACHAT0_000811"
     CDS             11557..11871
                     /locus_tag="ACHAT0_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8450775.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            11849..13543
                     /locus_tag="ACHAT0_000812"
     CDS             11849..13543
                     /locus_tag="ACHAT0_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8450776.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            13563..14741
                     /locus_tag="ACHAT0_000813"
     CDS             13563..14741
                     /locus_tag="ACHAT0_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8450777.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            14704..15390
                     /locus_tag="ACHAT0_000814"
     CDS             14704..15390
                     /locus_tag="ACHAT0_000814"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8450778.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            15390..16550
                     /locus_tag="ACHAT0_000815"
     CDS             15390..16550
                     /locus_tag="ACHAT0_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8450779.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            16560..17435
                     /locus_tag="ACHAT0_000816"
     CDS             16560..17435
                     /locus_tag="ACHAT0_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450780.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            17432..17743
                     /locus_tag="ACHAT0_000817"
     CDS             17432..17743
                     /locus_tag="ACHAT0_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450781.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            17733..18086
                     /locus_tag="ACHAT0_000818"
     CDS             17733..18086
                     /locus_tag="ACHAT0_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8450782.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            18076..18477
                     /locus_tag="ACHAT0_000819"
     CDS             18076..18477
                     /locus_tag="ACHAT0_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450783.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            18470..18874
                     /locus_tag="ACHAT0_000820"
     CDS             18470..18874
                     /locus_tag="ACHAT0_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450784.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            18886..19491
                     /locus_tag="ACHAT0_000821"
     CDS             18886..19491
                     /locus_tag="ACHAT0_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8450785.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            19511..19873
                     /locus_tag="ACHAT0_000822"
                     /pseudo
     CDS             19511..19873
                     /locus_tag="ACHAT0_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19876..20058
                     /locus_tag="ACHAT0_000823"
     CDS             19876..20058
                     /locus_tag="ACHAT0_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450786.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            20076..23495
                     /locus_tag="ACHAT0_000824"
     CDS             20076..23495
                     /locus_tag="ACHAT0_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8450787.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            23546..24283
                     /locus_tag="ACHAT0_000825"
     CDS             23546..24283
                     /locus_tag="ACHAT0_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8450788.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            24293..26584
                     /locus_tag="ACHAT0_000826"
     CDS             24293..26584
                     /locus_tag="ACHAT0_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8450789.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            26608..28689
                     /locus_tag="ACHAT0_000827"
     CDS             26608..28689
                     /locus_tag="ACHAT0_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450790.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSITSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SDYTFAITYTI"
     gene            28706..28855
                     /locus_tag="ACHAT0_000828"
     CDS             28706..28855
                     /locus_tag="ACHAT0_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450791.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            28852..29298
                     /locus_tag="ACHAT0_000829"
     CDS             28852..29298
                     /locus_tag="ACHAT0_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450792.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            29300..>29395
                     /locus_tag="ACHAT0_000830"
     CDS             29300..>29395
                     /locus_tag="ACHAT0_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8450793.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT        10422 a         4459 c         6119 g         8395 t
ORIGIN      
        1 ttatttcatc atttaaatct tctttctctt tttttaataa aataatatca ttttttaaca
       61 tagtaatttg tttatttaaa tctgatagtg tttcttcctt acttaatata aatctttctc
      121 tatcatgtaa tttatttagt ttttcatcat ttttttgttc taattcataa gcttcttttt
      181 ttattgtgtt agctttcgac agtttagtga ttcctataat taataaaata agtccaatca
      241 ataataacat tcaaaactcc tcatttcttt gatataatta ttgtagagtt tactcagaaa
      301 tgaggaaaga gtccatgtga aagcatgggc ttttttattt ttaatgttgt aatactaaat
      361 gttcaagatt atgaggaagc cctaaatatt caataatttg gaactgactc atatcagata
      421 aatcttgttc atctattgtt ctagttaaca tttcaattgc aaacatatta gcttcagctt
      481 ctataccata aatcatacaa ctaaggttgt ttgctctaaa agctgctgtg caaatacctt
      541 tatggagtct aacgtgggct atttcatgcc ataatacaaa tcttttcata gaaggtgata
      601 ggttttgatt aattaatatc acataacatc ttttattgtg tatcgtttgt cctaatgttt
      661 tattccctaa atcagtttca attaaatcta tatcagaata tttgattatt ttatatggat
      721 taatcgcatc agatccaaac tctacaaata atttttctat ttcttctaat atccagtatt
      781 tgtttaaatg cataccaaag cccacctatt gacgatattt tttaggagtg aattttttct
      841 ttgctttaac tttggttaag gcaagtgcaa gttgaatggc atcgtataat ttttccttat
      901 cttcatcgtc taaaggttca ccataataat ttgtttcagc atctgaattc aagccttcaa
      961 gtaatttttc agcttgtaaa gcaatatctt gtttatcttt ttctgttaaa tcataataat
     1021 ggcgtttatc agtacgtcct aaaagataat cagttgatac attaaagtaa tctgcaactt
     1081 tttgtatttt atctccagat ggagtacttt tattccatct agacatgctt ccatttccga
     1141 aatttaactt tctttcaagt tctgccaacg tcattgaacg ttcttttaat aagattttta
     1201 tacggtctaa tagcatagga tatgcattcc tttcaaaata ccgacaaaaa aagtattttt
     1261 ctgcaaatta tctatttaca aatagagaat ttgcagttat actagttttg taagcaagat
     1321 atttacggca tacaaagact aaaagcaata aactaatagt ctccccagac atcagtaagc
     1381 tcgctttttg tgcgctgtat tttgtatgct tacataatag catatttgca gtttttgtca
     1441 aacttattag acaaaaatat tagaaaatat tcgataaggg ggtatgaagc atgactttaa
     1501 aacagcaagt attaattgct ttagtaaaga aaggttggag tcaaagagag ttagctcgac
     1561 gaatgggtat atcaattact tatttacgag atatcttcat cgaaaaaaga aaacctaagg
     1621 aacgtttaaa acaaatcgaa gaacttttgg atatcaagtt agaagtagat tctaatgatc
     1681 agccatcaga acaggaggcg taaaaatggt acaacgtttg agcaaagcgg agtttaaaga
     1741 gaaatataag tattctgaaa gtacttatca aagaagaatg aaggaattta aaaaatcacg
     1801 ttttagtgag gggtataacg cagttacttc caaagaaata acaattgatg tcgatttata
     1861 tgaacagttt agaacatggc gttctaaaaa tcgatttaga gttagagctg tttgttaata
     1921 gctaaaaaat ctaaattcac cacttctgaa aagaggagtt gctgctctcc tttcaagttt
     1981 gccaaagtcc acgttttttc attttggcaa ctcctcttct tggaggtggt gacggcgaga
     2041 gacgaaggag ggatgacatg aaggaagaat caaaaatagg gattttgaat gagaacaatt
     2101 acacaagatt aattattgaa acagaagaag ggaaaaaagt agcagaagtt acattgaccg
     2161 atgcaactcc tgctgacggt tatcgtattc gattaactcc taaatatgat taaccttttg
     2221 gtgggtgtgg atcgtttccg tgactatcac ggctttgaat ttttccatct ctgccatgaa
     2281 taaatagctc agatccttga tttttagaaa tctctctagc gatttttact gcttctgatt
     2341 ttgtattagt atgtgcagtc gctttagaat ttccagcacc tttgacattc caaccaccat
     2401 tggaattagg tacaacatgt tgattttttc ccatagtttt cacctccatt tctatagaaa
     2461 tattttatca aatagaaatg gagaaattca ataaacgaaa ggagaagtga tatggcatat
     2521 accttgcaac aagaacatca aattctcggt ttgattaaac aacgcaggaa acaattacaa
     2581 gatgaccgtg cagcgcttag aaaagccgat gagctatcag ataggcaagc tgaactaatt
     2641 gcttctgaac ttgaggattt gagaatgcta gaaataaaaa atagggagat tagattatga
     2701 agaagacaga cacacttttt ataggattca ttttggggtt attagtgatt gtagcgcacc
     2761 aaagtattat cgggggaagc ttgttcgcag cattgatggt tttaatcaat ctgcttgatt
     2821 caaaagaaag gagcaactat ggcacgagaa gaagcgctaa aaatcggtaa agtgattgct
     2881 gataatcggt ggggaaatag ccgccctatt attttaagca agcaacatat cgaaaagcaa
     2941 aaagcatggc aacaaataaa aaagtgactc tgccagccag caaagagtca catacaaatt
     3001 tataaaacta ggagaattat atcacatgaa taagaaaatt gaaaatctta tcgaagaatt
     3061 gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt acagcccaaa aagaagggga
     3121 acttaaaagt ctcgtttatg gtgagacaac agaaatttta ctttgtcttg ctatgcaaga
     3181 agaacattta gatgaaaatc tcccattgtc agcacatatt atgcgtagaa ttgctgtgga
     3241 tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct tcaaatcata cttttgttat
     3301 taataacaag gaagatttag ctgatgtgat gactcgtatt ctcaaggggg aatttcaatg
     3361 atgaatgaaa aaacaattga tctactcatt attttatttt tcacgttagt gattgtagcg
     3421 aaaagttatg taatctttgg cttgttattc gcaattacca tgcttgtgct ttgcgtgtta
     3481 gcaagcagaa aagaggttaa acatgaacga gttagcaaat cttgacaact atttaactga
     3541 tcctgattat gctaaaccac catatgaagc accaattgat gaggaggatg aagatgagta
     3601 aatctacctt agaaatgagc catcaagaat ggcttgaaga ccgtaagaaa ggcatcggag
     3661 gttcggatgt tggaacaatt ttaggattga ataaatggaa atctccttat caactatggc
     3721 tagaaaaaac aggacaagta gtacttgaag aatcaggaag tgagcccgct tattggggta
     3781 atgttttaga agaagtggtt gctaaagagt ttcaggaacg tacaggcaaa aaagtacgca
     3841 gaagaaacca agtatttgaa catccattac atccattttt aagagcaaat attgatcggg
     3901 aagtagtggg agaaaatgcc attctggaat gcaaaacagc caatcaattt ctcggtaaag
     3961 aatgggaagg cgaagaggta ccgcttagct atctctgtca agttcaacat tacatgaacg
     4021 ttctaaacaa agactattgt tacatcgctg tcttaatcgg tggtcaaaaa tttatctgga
     4081 agcggattga acgagatcaa gagctgatcg atacaatcac tgaacaatta gtagagtttt
     4141 gggaaacaaa cgttcttgga ggtatcgagc caattattga tggtagtcaa gcgacagctg
     4201 atttcttgaa agagaagtat gcagatgttg aagacgttca aacgacgtta ccaattcatt
     4261 tcgatgaact agttgaacag aaaaacgaac tcaaacggac taagaaagaa attgaatcag
     4321 ctatccgtca agtagacaac gagattatca gcgagctagg taaacgtgaa gccagtatcg
     4381 gtatcactca aaaaaacatc atcagttgga aatttgtccg tacgagacgt atgaactcga
     4441 agaaactagc agagaaatat ccagatgtcg caaatgatga agagatttat aacgttactg
     4501 aatcaagaag gctaaccgaa agggagatca aataatatgg caacaaatga atcgttaaaa
     4561 aatcaattgg cagcaaagcc acagaaacaa gttgcaccag gtcaattagg acttaaagct
     4621 ttgatgaata caccaacaat gagaaagaag ttcgaagagg tgcttcatga caatgctaat
     4681 gcttttatgt cgaatgttat gactcttgta tctaatgaca gttatcttgc agatagtgaa
     4741 ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat taaatcttgg gctagataag
     4801 aatttaggtt atgcatattt agttccattc aatagtaaaa acaagcaaac aggaaaatgg
     4861 gaaaagaaag ctcaattcat gcttggctat aaaggatata tccaattagc ccaacgatca
     4921 ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag gagaactaaa aagctggaac
     4981 cgactgacag aagagtttga gtttgatcca aatggtagaa cgtctgatga agtcattgga
     5041 tatgttggct atttcgagtt actgaatgga ttcaaaaaaa ctgtctattg gaccaaacaa
     5101 gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg ataagacaaa gttaagtggt
     5161 gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg ttttgagaaa tcttctttct
     5221 aaatggggaa tcttgtccat cgaaatgcaa gaagccacca catcggatga gagagtccaa
     5281 agggttcaag aagacggcag cattattgct gaaacagaag ttgaagaaga tattcctgaa
     5341 agaaaagaag cagaggttat atctgaagaa aatgaagatg tacaaactgg attatttgat
     5401 gcatctaatc cgccgttaaa caaataatga gggagctttc tcccttgctt ttctagagga
     5461 gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa aagtgatgaa ttcattcgtt
     5521 tggaccaatc cttatatgta taaattatgg aacttgtgtt tgatgaaagc gagtcatgag
     5581 aatcgcaaga tactttttaa tggaaaagag atacaagtga acagcggaga attcgtcaca
     5641 gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga aacgtgaaca tcaagtgaac
     5701 agcggttctg tatggagatg gctaaaacaa tttgaaaaaa acggaatgtt gaacatcaaa
     5761 tcaactacga aatacagcgt tatatcaata aaaaactggt ctttgtacca aagcagtgaa
     5821 caacaaatga acatcaaacg cacaacaagt gaacaacaag tgcacacaaa caagaatgaa
     5881 aagaattata agaatgaaaa gaataataaa acatcatcgt cgcaaccacg caaaaagcgt
     5941 gtttacgaca ccgactcagt ttactacatt ctcgcggagg agttattcaa acagatttgt
     6001 cagaatcagg aaatcaaaaa gccgaatctg caaagttggg cagatgacat tcggaaaatg
     6061 atcgagatcg acaagcggac cgaaaaccag gtacggggaa tgattgaatg gagtcagcac
     6121 aatgtgtttt gggcatcgaa tattttatct gctaaaaaat tgcgagaaaa atacgacaca
     6181 atggcagcgc aagcaaatcg tgattataaa acaaaacaaa ctaaaacgct tgaatacgag
     6241 aaatttagca cagatgagtt gcctatttga gaggaggcgt aggcatggaa actgttgggg
     6301 aaatcatgga aaagctgata cagaaagtgc ttgtccaacg tggcgaatgt cctgaatgcg
     6361 gacagccttt gtatggatgg cgtacgaaga accctgatgg ctcagaacgt tgtaagccaa
     6421 cctgtatgca atgtgggtat aaagcattgc gagttcagga agacttacaa acagaacgaa
     6481 tttacaacga gagcctgaaa gcaagagcaa tcaatttttt caaaggtggt tctgttgtgc
     6541 ctaatcaagc gttgtttgat tgcacattgc agaattatca aattgtcgat caagaaacaa
     6601 gacaagcggt tgaagtaacc aaacgctttg ttaattcagt cttgttagga aatccaagtc
     6661 accttgtttt aactggaaaa caaggaacag gtaaaagtca cctagcgatg gcagcggctt
     6721 gggaagtctt gaagcgatca aactacgata aaaaaatctt atttatcggg ttacaggaaa
     6781 tgctggatca aatcaaattt tcgtacaaca atcctgaact cagaaaaacg attgagggat
     6841 cgttgattgc agatatcaaa acagcggatt tagtcatcat tgatgatatc ggttcagaac
     6901 taggaaaaga tgcatcagat agtcgagcgt ttggcataaa cacgctaaat tcgttcttgg
     6961 atgcacgaca gaacctagca acgattatca caacgaactt gcttggtgaa gaactgaaaa
     7021 aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa ctctgatgga tttacgatgg
     7081 tattttctca aacagcagac aagcgcataa aaccagtcaa aggagcgatc gcatgaattt
     7141 gactaaacaa gaaaaaagaa tctgtcgtca gataatgttg caaattatag gtgatcgtga
     7201 catggacgaa ttatctcaac aagagagtcg aatggttcgg aagataagta agctaattat
     7261 ttatcgggat acagaaagat ttctcactgt tttaggtaac gtctacgaga atccagagtt
     7321 attggaggta gagtaattgc caaaaatatt agatgcatgt tgcggtagta gattgttctg
     7381 gtttgataag aacaattctg acgtaacatt tatggattgt cgtcagcagt atgaagaatt
     7441 atcaacaggt catgtgatca atgttgatcc agatgtggtc gcagactttc gcgatatgcc
     7501 ttttgaagat gatgtgttcg atatggttgt atttgacccg ccacacttaa gacatgtcgg
     7561 cgaaaactca tggcttgcca aaaaatatgg aaaattagac gaactttggc cagaagacat
     7621 caggcaaggt tttgcagagt gtatgcgagt tttacgacct tcaggaacat taatttttaa
     7681 gtggaacgag gaacagatac cactttctga tgtgttagag gctattggag aacagccatt
     7741 gtttggtaat aaacgaagca aaacacattg gcttgtgttt atgaaaaaat tcagctaacg
     7801 gaggaaacaa aggaggaaaa gtaatgaaga ttgtattaaa taaatgtttt ggtggctttg
     7861 ggttgagcca tgaagcaaaa atggaaattt ttaaaagaaa aaatattgaa gtattcccat
     7921 acatcaataa ctttagctat gactcagatg atgaatacac gagatacaca ggccaaaagc
     7981 taggttcgat ggattttatc tactatttca aaaaagaccc gaaaatcgac aaagttacag
     8041 gaatttatag cgaaattgac cgactgtacg gaattgctga tgattccagt ttttcaagtg
     8101 attcaaacag aggtgataag gacctcgttg ctgttgtcga aaaactagga agtgaggcca
     8161 gtggaccata tgcaagtctt aaagtcgttg atatacccga tggggctgag tgggaaataa
     8221 gtgattatga cggcgttgaa actgcacatt atggttttca aacaggtagt atttagtcca
     8281 ctaatgacaa ctaaagagga ggcagaagga tgagtgaaga tgaatacttt gaacagtagt
     8341 ctattaacga cagagatttt taactggaaa ggggtgtagt catggacgca tcggaagtag
     8401 taaataaaat taatagtctc aaagaggttt tcggagacgt cgaagtagtg ataaacgcac
     8461 aaggctatga caacgaattt tttaagcaga ttaccgacat tagcattcag caaggaatgg
     8521 aagacgaaga cggaaacttt atcgatgaag tagccatttt agtgacttgc gagtaagtaa
     8581 ttgacagcta aagaggagga agcggaatga aactaaaaga cggattttac tccagcggcc
     8641 acggcatcgg cggtttaatg ctagatatgc cgacaaagaa tcctaaaaca cgtaagaaac
     8701 caaaattcaa agtcggtgac atggttcgct gcgaagcaga aggattcatc tatccatttc
     8761 gtggatatgt agaacacgtc tataatcact cagcaatcat tcgcattgaa aacacgatgg
     8821 aatgtgataa gtggttagcg aaaagcaaag agaatttagc agtggtgaga ttggtggaca
     8881 tggaagttat aaaatcttaa ttttgctttt ttacaactat cctacaacaa tgagcgttat
     8941 tccacaactg gaaggagaag aggcaattgt gaacatttta gagaatatag atattaagca
     9001 aacaaggaaa aatgctagac gattactaaa aagatacaga aatttagaac gtttggtcgg
     9061 accagtgaaa atagactttt ccgtgatgac tgttaccaaa aatttgaaat tcacaattga
     9121 cagtcaaaac gaagaaatca ttgaagcgat aagtactcga gattcggtta tcgaagcatt
     9181 aacacggcta agtagaatcc atttccaggt actttattat agttattgtt ttcctaataa
     9241 gatgtcaatg tatcaaatag gagaaaaatt aggatattcc gatagaacta tcgagagaat
     9301 gaaggcagta gctttggttg aatttgctga ggcgtataaa tcaggagaac tcatttcacg
     9361 tacaaaataa aaaaagtcgg attcctccga ctatgaataa tatttccgac ataagtatta
     9421 tatcataatt gggggaatca gaggatggta cttttcgatg taaagaaata tgagacacca
     9481 agcgcgaaag atgttgatat ggaacgcaca aaacataatg tcgctgtgtt tctttcagca
     9541 tatctatcag ctagatgtag agtagggcaa catcgagaac caaaagtgac agcatcttat
     9601 tccttggttc caccttctac agctgatcat atatttgaag cagaaagaat gctgatcgat
     9661 aaagaagaag cacaggaaga atttgagtat ctgcataaat tgtttgttcg aggctattca
     9721 gcgatacagc atccgcataa gcctgatgtg actgaaaggc gcaagaagat attctatgat
     9781 cgttatatca atggtctgcc catttatgta actgctcaaa ggaataatac tagcgaagaa
     9841 tcggttaaag tagaatcaaa cagaattatc atccaatttg cttcatcgtt agaactggtt
     9901 gctttcaagt agccagtttt tacacttttt ataccctttt attaccaatt tggtttccat
     9961 tttatacctt ttttatacca atcacttacc tattcaacgt tgtattatga tagtgtcgaa
    10021 aaattaggaa acaggatcga caaaataaaa tgtaagggag gaaatctccc tcatcgttta
    10081 attaagcttc gataggcagc aacggaaata ttaagaataa ggatgtgaat ttcaactcct
    10141 tctaaattgt tcttattatc tatcatccgt tgctgtctat tgttttttta attattcaca
    10201 cgataactaa aggtgagtga agagaaatga ttccattaat aatttcaatt tttgcgctct
    10261 gtcttaatgt ctatatgatt ggatttaaaa atgggcaaaa taaaaaatag tagcagccaa
    10321 gaataatttt atagtgtcac tgtggcggaa agggtagacg cttaaaaata aggtcaatac
    10381 gtcgagggat agccttaacg ttttatgatt tgaccatgca atgttcgatt cattgccagt
    10441 gactttggca accgagggtt ggaaatgggc gctcaaagta cacgagcaag gcgaggtcga
    10501 tagtaatcga tggaatcggt gtaggttgct tgatagagct ataactgcat ctcgtttatg
    10561 aggtgtagtt tttacagatt agatcactct ttgagtggtc tttttatttt acacaaagga
    10621 ggtaacaaga atgtatagac cacaatactt agaacagaag tatgaagtaa tcactgtgca
    10681 aaacggtaac ggtgagatag tacgaaagta tagaagacca ataaagagcg atacatacaa
    10741 acgaaaggaa agcaatgaag ttattccatt gtatggcaaa agaatagcta agcattaaat
    10801 aagattgcga aaggagacgg aatatgaccg aggaattcta tagatggcta ttacagttga
    10861 taagagaaga tcgtttagtt aagttctatc agtctcctaa atggcgcagg cttagagaga
    10921 aagcgatgaa acgagatcac tatgaatgcc aagagtgtag aagactaggt aagtatcata
    10981 gagtagaaaa cgttcatcat ataaaggaag tcaaggatag acctgactta gctttagatt
    11041 tagataatct tatttgttta tgtgttgaac atcataatga agttcatggc agatatctta
    11101 cagcattaga taaacaagag aagaagatag aaagctttgc taacttcgat gcaagtgaaa
    11161 ggtggtaagt gcatgatcat caatgataat ggcagagagt atgatacaga aaagattgaa
    11221 gagtattcat cttatactca gggattaatt aaacgtttga tatacgttcg ctatgtagat
    11281 attagggatc tgttatcaga taactgttgt agtaaataca aagtgaatca agtaagagaa
    11341 gcgttgaata aagataataa cgtcgaaaga ataaaaaatg tttttggata tagtattgaa
    11401 gagattaatt attacattga cttcgctgaa gctttcattc cgatggtgag ataacccccc
    11461 cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg agggggctgt caggaaaaga
    11521 gattttttcg aactttatca tgaaaggagg gctaaaatgt ttaaaaacga attgtctcaa
    11581 aatcgctaca gagaaaaatt acgccgctct ttaataagcc aattggaaag tcagaaaaca
    11641 aatattgagc cattcttaga taatgttgat cgttatatca gtttatggga aacggcgata
    11701 tcactggaag aagatatatc cgagaacggc attagactgg agaatggtaa aaagaatgaa
    11761 tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat tgatgttgga taaacttgcc
    11821 attactcctg aattggtagg tgaagcaaat gaatcaattc ctgagttata agcatattga
    11881 aaattggttc aaagctatag aagaaggcac tatcaaggta tgcaaagagc aattattgct
    11941 aaaaaattat ctagaagaaa gagtctttac tagagaagat atttacttcg ataagcagat
    12001 ggtagaggat tcaatcaata taccagcaca atactttcca ttcgaattaa ttccgtggga
    12061 aaaatttcta caatgtttta tttatggtgt tcgatggaaa aaagataaaa cactagtgtt
    12121 caatagatat ctttcattaa tgggacgtgg taatggtaaa actggttttg cttcttggaa
    12181 caacttcttt ctactaaccg ctaaacacgg tattaaaaat tatgatattg atatctatgc
    12241 caataatgaa agccaagcaa agactagttt tgatgatgta tttaaagtaa ttaaagatca
    12301 tcctgattta gataaaaaag tatttaaagc tacgaaggaa gttattcaaa atatcgctac
    12361 aaacagcaaa cttcgttata acacggcaaa tgctagaaca aaagatggga agcgaccagg
    12421 tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat tcaatgataa atgtggctac
    12481 ttctggtggt ggtaaaattc gagattatag agaattttat gatacaacta atggtcatgt
    12541 tcgtggtggt ccgcttgatg acattataga agaatcaaaa atgattcttt ctggagaact
    12601 tggaattgac aaggatggag cagaattttc tagtttgttt ccatttattt gtcgcttgga
    12661 taacgataat gaagttgatg atcccgacat gtgggaaaaa gcttgtccaa ctattaatta
    12721 caatgcagat ttaaaacgga aaatgtttca agaatactct caaatgcaac gtaatgctgg
    12781 tttaagactt acgttcatga ccaaacgaat gaacagacct atggaagata cacgatttgc
    12841 tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa gaatttcctg aaaaaatgga
    12901 tgaagtgata ggaacagtcg attttgctga tagacgagat tttgccagcg ttgggttgct
    12961 aggaaaatac gataaagatg tttattttac acaacatact tttatccacg aatcagccct
    13021 tcgattacaa aacatcaaac gagaggttat agatatttct atagatcaag gaaaatcaca
    13081 gatcgttcat ggaaaaaata tagaagctga ttatattgta ggttggtttc ttgaaatgag
    13141 taataaatat tatattaaaa aaatcgctat ggatatgtac cgtgcaaaaa tattgaagcc
    13201 cgctttagaa gaagcaggtt ttactgtgga aattgttcga agcggatctg ttacacatgg
    13261 tatgttaaaa gatctggttg atgacctttt tattaatcaa cgtttatttt ttggtgacga
    13321 tgcgattatg cgttggtatt gcatgaatgt atatgaagag catatttcta atggaaatat
    13381 acgctatgaa aaaatagaac ctgaaactag aaaaacggat ggcttttttt cattccttca
    13441 tggtttgaat tttttagatg atatttatga ttctgctcct gtaacagtca caaatagctc
    13501 agtagaaaat acaggaactg gatttactcc tctagtattc taacttgaaa ggaggtgaga
    13561 aagtggggat ttttcaaaag gcggtaggat acttcacaaa aaaagcaacg gttactttag
    13621 aagaatactt ttgtaaattg caagttgatt ttgtgtatcg aaaatttgcg attgaaactt
    13681 gtattgattt gattgcaaat gcgatgagta aagcggaatt caagtcatat gaagatggaa
    13741 aaaataaaaa gaatgatctt tactataggc tgaatgtagc tcctaataag aaaaataatg
    13801 caacagaatt tagaaaaaaa ctgatcagga gattaatatt ctacaatgaa gtattgatcg
    13861 tttctccgtc taataattct agcgaaatat ttattgcgga tagttgggat gtcacagaat
    13921 atgcattgaa agatgatgtg ttttctcaag tgcaaattaa caacatagtc cttgatagag
    13981 aatttctaga aagtgatgtt atctatataa aatacgcaga tcaacaaatt aggcaactag
    14041 tcgatgcgta ttatcaagcg tatgggaaac tcatttctag tgccatgaat gtttacaagc
    14101 gctctaacgc tcgtagatac gtactgaaag ggaatttatt ccgaccgcaa gacaatacaa
    14161 cacaagatca aatcaataaa atgatgacat cacaatttaa ggcttttatg gaagctgata
    14221 atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt agaagatttc agcggaaact
    14281 tccaaagcaa ttcaagagat ataaaaaact taatagacga catctttgag atgacagcag
    14341 cagcgtttca cgttccgaaa aacctactaa agggagacat gagtgggtta tcggatcaag
    14401 tggacgcttt tttaatgttc gaaatcatac cgattgctga acttattcag gatgcgttta
    14461 acgctagtct ctatgaagta gaagaatact tgtcagggaa ttttgtacgt gtggatacaa
    14521 ctatgatcaa gattactagc ttcaaagatt tggttgacgc tattgatgta ggcattagaa
    14581 atggagtatt tacaatcaac gaaggaagag aacgcgttgg aaatgatcgc tctgataagg
    14641 cgatggcaga tgaaatattt ataactaaaa acaatcaaca agtatcgaaa ggaggtgagg
    14701 cgaatgacga caatgaaaac atttctagca gtaaagaatg aaggcgcagt accgcaaatt
    14761 tttattcagg gatttattgg ttctagttgg ttctttgaag ggaatactga caagggaatc
    14821 aaaaatattt tggatagtct aggtgatcaa gaagaaattg aagtagtaat taattcaaac
    14881 ggtggagacg tatttcaagg gattgctatt gggaacttac ttaagtcaaa taaagcaaaa
    14941 gttaacgttg tgattaacgg cttagccgct agtgctgctt caattatcgc aatggctggc
    15001 gatactataa aaatctacaa caatgcacaa ttgatgattc accgcgcttc cacatacgga
    15061 gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac tggaatcaat tgataaatcg
    15121 gtaaaggctt catataaaac acgattcaat ggcacagatg aagcattgca agaacttctt
    15181 gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt atggattggt cgatgaaatt
    15241 atcgatgcag aaaatagctc aggtactgaa gctaaaaaag aacaaagcgt tgaagaaatt
    15301 ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg catttacagc agcattaaat
    15361 aaaacatttg gacaaggaga tgtaaaataa tgacagttaa aaatttaaaa ggtgtaacag
    15421 ctgcaagcga ccaattgatg aaagctttta aagatggtaa cgaagaatct tttagcgcag
    15481 ctatggtaag cttatctaag gaaattcagg ataaaatttt agaagaagca acagcaaaaa
    15541 atcaagatca attagtatta atgaaccgtg gtcagcgtgt attaactaca caagaaacaa
    15601 aattctataa cgaagtagtg aaaaacgaag gttttgcagg ggtcgaagaa ttagtgccag
    15661 ctactgtatt tgaacgtgta tttgaagatt tagaacaatc tcatccacta ttgcaaaaaa
    15721 ttacttttgt taacacaact ggtgtaacag aatggattgt gtcacgtgga gtcaatccag
    15781 catggtgggg taaactttgc gaagctgtta aaaaagtttt agataatggc tttgatgtaa
    15841 ttaacatgaa gcagttcaag ctatcaggtt atattcctgt atgtaaggca atgcttgatt
    15901 taggtccagt atggttagat cgttatgtcc gtactgtttt agtagaatca ttgagaattg
    15961 cattagaaca agcaattgtt gatggtactg gtaaagatat gccagtcgga atgatgcgtg
    16021 acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa agcagaacct attacagctt
    16081 tagatgctgt aactatgggc ggtttgatgg cgcgactatc aaaattcaat atcgaaggtg
    16141 tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt ggtcctaatt gtgaatccaa
    16201 ctgattactg gtctaaagta ttcccagcta agactgtact aactgctaat ggagaatacg
    16261 tacaagtatt gccagtacca gtttcagatt tgcagtcaac ggctgtgcca gaaggaaaag
    16321 cagttattgg ggtagcctca gattacttca tgggtgtagg atctacacta aaaattgaag
    16381 cttcagatga ataccatttt gttgaagacg aacgcattta tctagctaaa caatatgcaa
    16441 acggtcaacc taaacgtaac gatagtttca ttgtattaga tattagcgct ttgggaacta
    16501 ctactacaac tacaaaacca acaaccacaa caactacaac acaagcgtag gtgatcagaa
    16561 tgaagtatat tctttgtcag ccggcaatca atcggtttaa atgggagctt gaagtttgtt
    16621 taactaatct gaagaaacta ggaatcaaag atatcgtatt gcttttcagc agacacgatg
    16681 atcagattcc tatttttttt gagaaggaat atggtgttga agttcatgtg tacgatgatc
    16741 tgcgggacga caaagagtat attccttcga ttaaaccata tttatggtgg aaatatttag
    16801 aagaagatca ttcgcgtgag gacgaccgat atttctatat cgattcggat gtcattttca
    16861 ataaaagaat taatttgcgc aaattgcctt ctaaagatga tgtttggtat tgtagcgact
    16921 gctgtagtta tctaagtctt gattatatta gaagctgtga aaacggagaa aatattctaa
    16981 aagatatggc aaacattgta aatgttacag tagaatcttt ggaaactata aacactaatt
    17041 caggaggcgc acagtgggtt attaaccgcc ctaaagcgaa ttattggaaa aaggtttatc
    17101 tggattctaa tcggctatat cgctacctta gagggcaaaa aacaaatata aaaatctgga
    17161 cagccgagat gtgggcacag ctttggaaca tgatgtattt caatattggt cctaaagttc
    17221 acgaggaatt agacttttgt tttgctactg atccaataga aaaagttaaa gaagtaaaaa
    17281 tcttacacaa tgctggagta acaacaaacg atgaagattt atttttcaaa gggagatacg
    17341 tgacttctac gccttttgat gaagatttat catttgtaaa caagaaaaaa tgctcttacg
    17401 catatgttaa agcaattaag gcggtggtta gatgacgcct gaacaagtga ctgaagaatt
    17461 gctaatagct gtgaaggata atatttacgt tacctggaac gaagaagatg agtcaattaa
    17521 aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa gtgagtacaa cactttcttt
    17581 ttctcctgaa agcttagaat acggattgct aatcgaaaga tgtagatacg actggaatcg
    17641 tgctttagat gagtttgaac aaaatttcgc tagtgagtta ttaggtttca ttcaacatta
    17701 tgcgctacaa gaatatattg caggtgatgt gaatggcgaa taatcgtaga ctcgaagaaa
    17761 cattcaacga tggttggtta aagattttga cgcaaactac caaaagaaat gaactaggaa
    17821 aaaagattgg tgtagaagat acagaaatca cttctttaaa atttagaaat ctttccatga
    17881 gagatagtga tataacagct atggatgcga tgggatcgaa attaactaag aaagtaaaga
    17941 ctccatttca tccaatcgcc aagaaattta ataaagatca atattttatc gtaatcgata
    18001 gtatgcgtta caacgttatc tatgccgatt acgataattt ttatatctat ttttatcttg
    18061 aaagtgtggg tgaatatggt gattgataat tctaaagaaa aagaacgttt aaataagcaa
    18121 atttctgcta tcaaaacttc cttagaagag cattttggcc tcaaactctt tcaagactcc
    18181 gtaggcgagg atgagctacc tgatgatttt aattacttca ttctcgaaac aggagaaata
    18241 gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac tttctattca
    18301 gaaaatagag aagatttaac aggagattca ctagatatta tttcattgat tcaaaatcgt
    18361 tcgattcgtt ttcagagaat ggatcccaat catttaaaac tagagaacca agatcgctat
    18421 atcgatcaat tggtatttac gtttagacga ttattgaaga gtgattgtca tggctaaaaa
    18481 tagttgggag ctaaaaataa atggacatga tgaacttctt gtgcggatgg aacgctattc
    18541 aagcgagagc gaacgactga ttaacgaagc attgaaatca aaaggttcgg ctattgcagt
    18601 ggataggatt acagaaaaaa ttcctgtttc tgaagcagat ttaagaagag ggcaccaaca
    18661 cgcaaaaaat agtcgtccac ttaagactca atacattaat ttgggtttca tcattagacc
    18721 tacaagaaaa tttgagtatt taaaatatcc tgatttgggg ataggtactt ctaaaagaaa
    18781 tcagccggac gaatttatga gaagaggatt aggtcttgca cttgatccaa ttacagaact
    18841 tctgattcgt caattcgata aattaaataa ataggaggaa caacaatggc taaaacaaca
    18901 acagtaacaa cttttgacaa tataagtatt aaaagaattt cttttaattt taagaatgca
    18961 acaaatgcaa tttcaactga ttgtaatgga caattagatg gcgaaacaga aatgcagaga
    19021 attgtaaaaa aatgcggttc aacagaagta aaatcgaaat ctaaaccaat caatatgacg
    19081 gtaacaatta ctgcacatgt accgatggaa gtttatcgac gcttcaatgg gttgaaacaa
    19141 gatgaacgta ttaaatcggg aatttactct tatggtcctg attccgtagg ggaagatttc
    19201 tctcttgctg cagagatcgt ggatgatttc gaagaaaaaa ataagctgat tggtatgtta
    19261 gcatgcactt cgaatacagg attaacattc tctattgaaa atggtgcgga tgaagtagct
    19321 gcgttagaac tagaaacaaa agttatgcaa gatgaatttg gtaaatttta tcatgaagca
    19381 attgttgcag aacttgaaga agacttaaca gatcaatgga tgacgaatct atctgctgat
    19441 gtgattaaaa aaacttcagt tgtgacaact acggccactc aatcacagta aaaaaaacgg
    19501 aggtagcgaa atgaacgaag attactcaaa aattgaacta aacgatggaa caattttgaa
    19561 tttagaacct aaactgaata tcaagaaatt attgatgatc aatagagatt ttaacacaga
    19621 cgagtttgca aaaatgtcga tgggaaaagg ctctatggat attacagtta ttcaaggtgc
    19681 aaaagccgta tatgtagctt atcgtcaagc gaacatggtc gattacattt cattcgatga
    19741 atttatagat aaatgagatt ttgatatgga ggttgcagta gctgtataca gtactatgat
    19801 gttcaaacaa gcacgtgatg cctatcaaaa agaattcgaa aaagcaaata aggaaaaaaa
    19861 gcttcaaaag taaaaatgcc aaagctctta gttgaaacgt gggtcgatgt ctattcgatg
    19921 ttgaccgacg ttttttctat gccttcagat ttggttttaa gcgatatctg tttagatgac
    19981 attttgcaaa tggcttacaa caagagcgct tatgaaggat ggaagaacta tgcaataaat
    20041 caatcccaga aaaactaaag gaaaggaggt aaaaaatggc taaaaagaga acggaagcag
    20101 aagtaacttt catagctaac gatgacggat tgaaatctac gttaaaagaa atcagtgctg
    20161 aattaactaa aaatagagca gaattaaaac tagaacaagc tcaattgcaa cagactggtt
    20221 ctgaatcaga caagttagga agtaaattat cttctttaga aaagcagtat gaattacaaa
    20281 gtcaaaaagt tgaagtaact agccaacgtt tagccaatgc gaaaaaatat tatggagaaa
    20341 attccaccga agttcagaaa cttgaaagag aactgattaa tcaacaaaca gcgcaacaac
    20401 gtttgtcaaa cgaaattgat aaaacgagta atgcactagc tcaagcaaaa ggcgaaatac
    20461 agacgtacga gtctacaatg caacagttgg atagcgaaca aaaaaatgtt caagctagtg
    20521 cttctctgat tgaatccgaa tacaaaaaat ggcaagcaac tgctggtcaa tcagcttctg
    20581 aagccgagaa attagcgaaa gcccaagaat atgtttctca acaatctgaa aatgcggaga
    20641 aaacgataga tatcctaaga cgacagttag aagctacaca gtctgaattt ggcgctacat
    20701 ccacagaagc aatgcagatg gaagcgaagc ttaatgatgc tgaacgtgaa tttgaagagt
    20761 taggacaagc tgctaaaaat gtagatacaa ctaacttgga cgatatcgga agcaaaatag
    20821 atatgaataa tttaatggaa gcttctgacg ttttaagcga cattggcgat aagcttacag
    20881 aattagggaa acaagcagtg gactctgcta acagtgtagg tagttcccag agtaagatac
    20941 aagctaattt tggtttgtct aaacaagagg ctgaagaatt aacgaatgta gccagagaca
    21001 tttattataa aggttttgga gaatcgttag atcagtccac agatgcattg attttggtaa
    21061 agcgtaattt aggagattta aataatcaag atttacaaaa tatcacggaa caagctatgg
    21121 tcctagaaaa caccatgggc gctgatatgg atgaaacgtt acgtggtgta aatggcttaa
    21181 tggtcaattt cggcttgagt gctcaagatg caatggattt aatggtttcg ggtactcaaa
    21241 acggtttaga taaaacgcac gaattaggcg acaatatggc agaatatagc caattatgga
    21301 gtcaaatggg atattcagct gatgaaacgt tcggaatgct tcaaaatggt ttagatgcgg
    21361 gtgcttataa ccttgataaa gtcaatgact tagttaagga aatgggaata tcgttaacag
    21421 atggtcgatt tgagcaaaac atggatatgt ttagtgaaag tactagaaaa gcttttgaag
    21481 agtggaaaaa tggcggagga acacaaaaag acgttattaa ttccatgatt caagatttta
    21541 gcaatatgga tggtcaatac gaccaattaa ataaagcttc gacaatttgg tctgcgcttg
    21601 gcgaagataa tgcgatgaaa gttgtccaat ctttaactga tgttaaccat acatttgatg
    21661 atgttagtgg atctgcacaa aaaatgaatg aagattctac tactccgtta caagaattaa
    21721 acggaaaaat agctgaatta aaggattcat tagctcctat aggcaacaca atcatagatg
    21781 cactcgaacc agtaattgat tttctaggaa agatggctga tgcgtttaat aatcttccac
    21841 aaccagtaca ggattatgcc gtagcgattg gcggattgac tgctgcattt actttattaa
    21901 tgccaataat agttggcttc atggctctag gtggtcctac tacattaata ataggagcag
    21961 ttattactgc tattgctgga gttatagcaa ttataaaaaa ctggggcgca attactgact
    22021 ggtttaaggg aatatggagt aaattcactg attggttggg tggtacttgg gaaagtataa
    22081 aagaaggtgc ctcatcagtt tgggatggag ttaaagaaac ctggtctgga tttgtagatt
    22141 gggttcaaga tatttggcaa ggagtttctg attggtttgg agagctatgg agcggattag
    22201 ttgaaggagc ttccaacatc tggcaaggag tccaagaaac ttggcaaaca ttcgttgatt
    22261 gggtttcaaa tatttggaac ggagtcaaag aagtatggtc gattatttgg gcagacattg
    22321 taggaattgt tcaaatacca tggacattaa taacgtcatt gattcaagct ggtattaata
    22381 ttatcgtggg tatttttgat gtagctggac agttattagg cgcagcttgg caagctgttt
    22441 ggacacctat ttctgatttc cttaaaaata cttgggatac tatgacacaa tggataagca
    22501 tcgcttggaa cggaattgta actacattcc atactatatt tgatccagta gtggcatggt
    22561 ggaatggtat atggacatct attagtacta cggcttcaaa tatttggaat tcaattagtg
    22621 caacagcttc tagtatttgg aacagtatca agaatacaat cactagcttg gtacaagcag
    22681 ctgctacagt aattcaaaat atttggtcaa ctgtatctag ttggttaggt ggaatttgga
    22741 attcaatcag ctctacagca tcaaatatct ggaatagtgt gactagtagt ataagcaatg
    22801 ctataaacgc agctaaaagt gccattcaaa gtgtttggaa tagtatatct tcgtggatca
    22861 gcggaatttg gaacggtatc aaaaacactg ctttgaatct ttggaatgga attacaagca
    22921 ctattagctc taaagtaaac gatggaaaaa atgcaatttc aagcggttgg tccaatctaa
    22981 caggtattgt ttccgacata ttcaataatg ttaaaagtac aattgctaac atttgggaag
    23041 gtatcaaaaa gactgttagc gctccgattg attggattag agataaaatc agtggcatct
    23101 ttgataattt gaatatttcg ataccacata ttccgttacc acattttaaa ttgagtgggg
    23161 aattcaatcc attgaaggga aaaatcccaa cgttgggtgt tgattggtat gcgaaaggta
    23221 gtgtgtttaa ttctccgaat attatcggtg tcggtgaagc aggacctgaa gcagttttac
    23281 ctttgaaaag atctgttttg caagaaattg gtgatcgtat cttgagtagc acatcagttt
    23341 catctagggc acaaacgatt caacctgtga ataactacga attcaatttc acaattgatg
    23401 gtaacgcaga tgaggttact atgaagcaaa caactcaaca aatcattgat agcattacaa
    23461 aaattcaaaa tgataatgct tcggcatggc gttaaacagg agagtatttc tcctgttttt
    23521 ttagtattaa aaaggatgtg aaaaaatgac tgattgtata cattctataa tcgatggatt
    23581 tcctgattat ttgcataaat tggctttagc tgaaagacca accatacctt ctccaaaaag
    23641 acagagagtt gaaacttctg ttttaggtag gttaggtggc ttagtacaag attactcgtt
    23701 tgaagatatg tcgtttacat tgcactataa ctatttagag gatgtggaag accatcaagc
    23761 gttcaagcaa tcgttttata tcatgcgtca ttggttaaac tatgcaaaga aattagaatt
    23821 ctctgacgat cccaacgtct attacgttat ccagactatc gatattgggg atgcagaaaa
    23881 cgatattgtt gaatggggag agttcgatgt aaatattact gcgaaaccat tcgcaagagt
    23941 tcaagaagat gtacctataa ccgtagataa accacagtca tttaacttgc tgaataatag
    24001 tttagaagaa agttttccaa agattatcat cactccttca gctacttcat gccagttcat
    24061 cttaaatgat tatgtgttta gtttcgaagg cttagtagca ggaactgatg tagtcattga
    24121 tagtgatttg atgctttgct acgaagagca atcggacgga gatattttag atcggtccaa
    24181 caaaatgaag accatgcaat atccaacgtt gcaagtggat attaatcatt ttaattgtac
    24241 tggtttgagc aaaatacaaa tttatcgtaa cgggttaagg taggtgaaat agatgatcga
    24301 taatttaata actatttacg ataaaaatga cgcgaataat ttagctgaac atttatatga
    24361 tacgcaaggt ttaggcgctt tgtcagactg gttaacagct actgttagca ataaactaaa
    24421 cggagccgag atatttcagg gtacttattc aataaacgga actaatgcag atttgattgt
    24481 agaaggacgt attattcagt gttatgtaga tgaaaatcga gcaaaacagc gtctacggat
    24541 ttattatgca aaaacttctg taataggcaa tacgatagaa gtaaaagctg aacctatttt
    24601 caatgatata agaaaatcgg tgttgaataa atatgacagc ggaacagaaa agatcactgc
    24661 tactcaggca tggcaaaacg caaaagtttt agcgaaacca gctatccctt cgcagttttc
    24721 tttctcgtca ttagtagata cgcttgctaa tgtgaagata gaaaaggcga attttttaga
    24781 attctttggt ggaaaagagg gatctattct agatcgattt catggtgagt ttctaaaaga
    24841 taataacaca ttacgtcatg aaaaaagtct aggtacggat cataaaatca aagcgattta
    24901 tactaaaaac ttaactggtc ttgacttaga gattgatgct caaagtgttt tagttggagt
    24961 ttatccattc attagcagct cttcagaagg agaagacgag atcactctac cagaagaagt
    25021 tattttcacg gattacgtgg atgattatcc tgctggatat gtttcttttg ttgattttaa
    25081 agacaaagcg actgatgtag ccacattaag ggaagctgct aaagactggt tgaaaacaaa
    25141 catagataaa caaaaaccac aagtgagtgg ttcgattgaa ttagtaccat tgaggcatca
    25201 aagaggctat gaaaaatttg ttgatctaga aaaagtttcg atgggtgacg gagtagatgt
    25261 gtatcatcca cagttaaaag tgaatatgtc agcgagaatt gtggaatata cgtttaatgt
    25321 tctaaccaat tcatacgata aattagttgt aggaaacgtc aaaacaaact tcttagaaaa
    25381 cacagagaat aatgtcagca atttgattaa tgatgccatt gatcaattga aaaatggtgg
    25441 cgaaatcagt gatttaatca atgatattgt agatcatcaa actgatatga ttactggtca
    25501 aaatggtggt tacgttttat tagatcctaa agaagcccct agtcgtattt tgattatgga
    25561 cacaccagat aagaatactg cacggaacgt tttgcaaatc aacaacgctg gtattggttt
    25621 ctctaaaact ggcattaatg gaacgtatga caccgcatgg acgttagatg gcggattcaa
    25681 tgcctcgttt attacagctg gtgagatagt agggattact attagaggta ctacattaat
    25741 tagtgatggc actgattata gaacaagtat agctaatggc aaaatgactt ggtactcaaa
    25801 aaaagttaac aaagatatta tggagctaga agcacgtgat tatgtaagtg ctgatgcagg
    25861 tattgtatca tacaccatga aaactggtgg tggtttcatg attagaaatc cacaggggaa
    25921 tttggttttt agtacgtggg ataatggtaa taacaaaccg tttttatctt ttggtgcgcc
    25981 caatttcagg tatagcaatg ctagttatat aactgatggc gatggtagtt ctttaagcat
    26041 taatggtagt gcgggcagtt catgggagtt taaagtagct ggtaggacta tgaaatttac
    26101 tagtgatggt atgctaacgt tgccaggttg tttttttggt tcatgggaag atggaaaact
    26161 tgctaggttt gaacaatcaa cggtacaagt atataaaaat tttactgtta gaggtactaa
    26221 aaactcaacc gtaccaacag aacattatgg acaacgacta ttaaacgctt atgaaactcc
    26281 agaatattat ttcgctgatt atggggaagc cgttacaggt gatgatggta aagttcgtgt
    26341 tgatattgat cctatgtttg ccgagacagt aaatctaagt cgatatatga cacatgtgac
    26401 acctacagag ctagttttgt gtgctgttac tcatgaagat attgaccatt tcatcattga
    26461 aactagtaag ccaaacgtat tggttagatg gaatttagtg gcacatcgac tagggtatga
    26521 agatgttaga ttaaaagagg atacagcata tgatagcaca gtgcttgacc aaaaacgttt
    26581 ttaaaacgaa gacaaggagg tatataaatg gctagcagtt tatataattt ggctttagat
    26641 ttcagcaaag aattaaacta caccaaagct attatggctc gtcaaggtga caaaggaatt
    26701 acggtgactg ttaaaccgtt tttaaatggc ttgcagatgg atacgagtgg cggaaccttt
    26761 acattaaaag gaacaacacc atctaaccgt tacgtagata gtgtcgctac tagtataact
    26821 agcgaagaag tcacattttc tcttgatgga acatttatga gtgaagcagg atactataaa
    26881 cactgctacg tagaatatag aaaagataat caaattttaa cgacgcaaga tatcattttt
    26941 ttctcactag gagtgtctga catttcgcaa ggccaagccg atgaatatgt ttcgcaatta
    27001 gaagaattga ttcgaaagta taacgaaact tttgatgctt ttctggctga aattaaaggt
    27061 agaatggata gcttaaataa acagattact gatttaactg gtcaagctaa aacactacaa
    27121 gacaagttag atgctctgaa agaagaaatt tctaaattag gtaacttaca tgtgatgtac
    27181 tccaacagca tcgacttcgg gggctatgat tatagtggga atccgaattt aatgtccaaa
    27241 ctaaaatcga gcgattttaa cgttggttac cacgggtcac taactaatgt taacggaatg
    27301 ttacatttta tgtctgatgg tacaggggac attgatatgt ttacgcgtat taatacacct
    27361 cagcttgcta gtgggaaaac ctatactctg agtgcgaaag ttcgatttga tgaaggaact
    27421 acaggagcta ttgataaatt acgtttggtg tatcgtacat caccaggaga aaagatatta
    27481 ttggaagcaa atagtacaaa tattacaaca gatgatgtag ggaaagaaat aacaatcaaa
    27541 ggtacagcta acgttaatta tcaaatcaca aatttagatc gattttatat tagtgttagt
    27601 ttttctaaca gggataaaat aaatggtgga tttaaattgt acgacatcaa aatcgaagaa
    27661 ggctcaacag ccactccgta tcagccaaac ttactagatg acccttactg ggtaggtaaa
    27721 acatcgttgg gtaagaatat tgcaaataaa ggtacgacgt tcccaattaa atctagcgcg
    27781 tacgaaatat ataaaggtaa cacggaagaa gagcttataa taggtcaaac gtatactatc
    27841 acgcttaaag gaacaaaacc cgcaagtcaa acctttgtag catataatta ttggaacgtt
    27901 aattttggag atctaaaacc agttgaggga ttgacagacg tatggtctct aacattcaca
    27961 ccaacgaaac ttgaaccggg tttgcctaaa gaccttcgca tttttcagtc gcctaaagaa
    28021 acggccggcg catgccgaat tgactggctc aagatcgaaa aaggcgacac acgaaccccg
    28081 aatattgagc aatataaata ccgaggaatc ggcatgcgag actcaaacaa tccaaaagat
    28141 tatgtatggg atctagaacc tagctatgtt gagaataacc tagctacaga tatcaaggtt
    28201 gctgaaatca caggtaaagc aaacaattat accgatggga aagtatcgga gattaattcg
    28261 cagttgactg cttcaattaa tgaagtagac accacagcta aggatgctca aacaaaagcg
    28321 aatgctaatg cgactgctat agatgaatta gacaataaga tcgatgaacg cattaatgat
    28381 acagctacta ccacattaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
    28441 gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaacgcag tggtggaaat
    28501 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
    28561 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
    28621 gccattaaat ggcgaagaaa ctcaagttat ggatcggatt atacctttgc aattacttac
    28681 acgatttaga aaagcgtgaa tcgatatgaa ggcagcatat agaccaattg aaccttacgg
    28741 attcgagcaa atcattgtga atgatgaaga acatttaccg gaagaatgca cagaagtcga
    28801 accaccgatt ccaaattgga aaccgaaatt caattactgg gagggaaata aatgaaaaac
    28861 atttggaaat atggacgtac tggcggagag tacgcaggaa aagtattgga cgacatgctt
    28921 gtatccgttc cttacacaga tcagccaccg cttgaaggaa ttcgtgctga tggcgaaccg
    28981 ttaacaatcg ctgatcaaat gtttgatcct aaattgaatc aatggattat tttagcgaac
    29041 gcactagatc acaacgattt aaacaatctc aaagcaatgt atgagtcgtt agaaaatgag
    29101 aacggcgatt taaaacagat caatgccaaa ctcatgctaa gcgatgtagc aattaaacag
    29161 gaaaatactg cattgaaaga aaaagcggat agtttagcac aaatcaattc aaaaatgatg
    29221 cttgcttcgt tacaaaatag caaagacatt tcagaaatta aagagcaact aaatccagct
    29281 tcaaagggag gtgagtagta tgtttagttt tagcgatgtg aaaatgatgt atgattgggg
    29341 ctgtttcact aacgaacaag taatggtttt cgttccgttg tgcattactg aagaa
//
LOCUS       DAYYAR010000021        29291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000021 DAYYAR010000000
VERSION     DAYYAR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.021"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..256)
                     /locus_tag="ACHAT0_000831"
     CDS             complement(<1..256)
                     /locus_tag="ACHAT0_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8450794.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNE"
     gene            complement(289..1374)
                     /locus_tag="ACHAT0_000832"
     CDS             complement(289..1374)
                     /locus_tag="ACHAT0_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8450795.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            complement(1393..2433)
                     /locus_tag="ACHAT0_000833"
     CDS             complement(1393..2433)
                     /locus_tag="ACHAT0_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8450796.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            complement(2449..3840)
                     /locus_tag="ACHAT0_000834"
     CDS             complement(2449..3840)
                     /locus_tag="ACHAT0_000834"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8450797.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            4218..5201
                     /locus_tag="ACHAT0_000835"
     CDS             4218..5201
                     /locus_tag="ACHAT0_000835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8450798.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            complement(5569..7707)
                     /locus_tag="ACHAT0_000836"
     CDS             complement(5569..7707)
                     /locus_tag="ACHAT0_000836"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8450799.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(7746..9332)
                     /locus_tag="ACHAT0_000837"
     CDS             complement(7746..9332)
                     /locus_tag="ACHAT0_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8450800.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(9322..10542)
                     /locus_tag="ACHAT0_000838"
     CDS             complement(9322..10542)
                     /locus_tag="ACHAT0_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450801.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(10554..11360)
                     /locus_tag="ACHAT0_000839"
     CDS             complement(10554..11360)
                     /locus_tag="ACHAT0_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8450802.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(11570..12850)
                     /locus_tag="ACHAT0_000840"
     CDS             complement(11570..12850)
                     /locus_tag="ACHAT0_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8450803.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(12894..14927)
                     /locus_tag="ACHAT0_000841"
     CDS             complement(12894..14927)
                     /locus_tag="ACHAT0_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450804.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(14961..15812)
                     /gene="rfbD"
                     /locus_tag="ACHAT0_000842"
     CDS             complement(14961..15812)
                     /gene="rfbD"
                     /locus_tag="ACHAT0_000842"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8450805.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(15910..16938)
                     /gene="rfbB"
                     /locus_tag="ACHAT0_000843"
     CDS             complement(15910..16938)
                     /gene="rfbB"
                     /locus_tag="ACHAT0_000843"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8450806.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(16960..17532)
                     /gene="rfbC"
                     /locus_tag="ACHAT0_000844"
     CDS             complement(16960..17532)
                     /gene="rfbC"
                     /locus_tag="ACHAT0_000844"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8450807.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(17546..18412)
                     /gene="rfbA"
                     /locus_tag="ACHAT0_000845"
     CDS             complement(17546..18412)
                     /gene="rfbA"
                     /locus_tag="ACHAT0_000845"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8450808.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(18512..19219)
                     /locus_tag="ACHAT0_000846"
     CDS             complement(18512..19219)
                     /locus_tag="ACHAT0_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8450809.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            complement(19224..20051)
                     /locus_tag="ACHAT0_000847"
     CDS             complement(19224..20051)
                     /locus_tag="ACHAT0_000847"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8450810.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(20051..20851)
                     /locus_tag="ACHAT0_000848"
     CDS             complement(20051..20851)
                     /locus_tag="ACHAT0_000848"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8450811.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(20844..21983)
                     /locus_tag="ACHAT0_000849"
     CDS             complement(20844..21983)
                     /locus_tag="ACHAT0_000849"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8450812.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(22188..23381)
                     /locus_tag="ACHAT0_000850"
     CDS             complement(22188..23381)
                     /locus_tag="ACHAT0_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8450813.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(23460..23666)
                     /locus_tag="ACHAT0_000851"
     CDS             complement(23460..23666)
                     /locus_tag="ACHAT0_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8450814.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(23659..24801)
                     /locus_tag="ACHAT0_000852"
     CDS             complement(23659..24801)
                     /locus_tag="ACHAT0_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8450815.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            25313..25948
                     /locus_tag="ACHAT0_000853"
     CDS             25313..25948
                     /locus_tag="ACHAT0_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8450816.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            26084..26176
                     /locus_tag="ACHAT0_000854"
                     /pseudo
     CDS             26084..26176
                     /locus_tag="ACHAT0_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            26290..26925
                     /locus_tag="ACHAT0_000855"
     CDS             26290..26925
                     /locus_tag="ACHAT0_000855"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8450817.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            27032..28270
                     /gene="mef(H)"
                     /locus_tag="ACHAT0_000856"
                     /gene_synonym="mefH"
     CDS             27032..28270
                     /gene="mef(H)"
                     /locus_tag="ACHAT0_000856"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8450818.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            28497..28916
                     /locus_tag="ACHAT0_000857"
     CDS             28497..28916
                     /locus_tag="ACHAT0_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8450819.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            28921..29160
                     /locus_tag="ACHAT0_000858"
     CDS             28921..29160
                     /locus_tag="ACHAT0_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8450820.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         8963 a         5782 c         4957 g         9589 t
ORIGIN      
        1 tctcattttg taatgctctc tttactaaat tgatttctgc ttgtacaatg gaaaaaccac
       61 cccaataaat tttaacacgg tttttattac ataaaaattc aacattatca tatgatttaa
      121 atgcttcaat aaatggttct atatcagttt ttttatctat atgaataata aaaactgcat
      181 tttcgttcaa tcgattgacc aatctcttta agttgccaat atcattgtat gccaatatca
      241 aataacatat tttcaaaaat aatcactcct aaaatatctt gaacattttt aatgatctct
      301 actatatata ctctccattt gttcagtagt gtacgaaaca tcataactct ttagttcttg
      361 tgttatttta ttagcatacg gctcttttat tacttttttt gcccattctt gtggtgtttc
      421 atctaatgat acaaaatcca cattatttgt taactttgta tctttggaaa tagtatctgc
      481 taagactaat tttaattttg ctgcttgtgc ttctaatcca accattggca gaccttcata
      541 aagagaaggc attacaaaaa catccatcac agataataac tcgggaatat cgtttctttc
      601 tcctaaaaat tttactcttt tctctatatc caattctact gctttggctt tcagtttatt
      661 tctaagtgac ccttcaccta ctaaaatcaa gtaagaatca gaattcattt ttagtatttc
      721 taaaaaaatc tctaacaaat aagattgatt tttttgtttc tctaatcgtc caatgtttcc
      781 cacaacaaaa gtactcattg agatatccaa ttcttttctc ttattaacct tagcttctga
      841 atttttacta taaagctcta aatcaatacc attatgaact atctcaaagg ggtggttttg
      901 aaacagactt tcaccagctt cttgactaac ggcaaggtag tctgttgcat tttgtaacaa
      961 caatttggtt aaaaatttac gcaatacatt cattacaata ttttgagatt ttccatcttc
     1021 agttgcatgg ctatgagcaa ttctctgtct gattccattc tttttggcta accataaatt
     1081 aattcccccg ccaaaataaa cctgattgtg aactatagaa aaagattctt tttttagtaa
     1141 cttattcact tctattaaat atttaaaaat atttttctta ggattaggta caacaaaaat
     1201 acggcctcct aatctttcaa tctctgaatg atagtcttga acatctgcat aatcttcata
     1261 aatcaaaaaa tcaaattgat agtttgtacg atcaatttta cgatataaat tcatcatgaa
     1321 agactctgct ccacctaaac ccattcgtcc ctgaaaatgc aaaattcttt tcattatatt
     1381 ttcctccatt aactaatata ctcgcttcaa agcagccatt aaccctggca ttttcacttc
     1441 aacgaagctt ccaactttta cagcagataa aaatagtgga gctggcatat tgtaaagcaa
     1501 ctttgttatt ttttttgctg aagaatccgt cttagcaatt tgcttggctt ctcggatcac
     1561 tggatcactc aatatctgct ttgcttcgtc tttcttctct tttgaagttc ttgcagaggt
     1621 actataaata gaaaaaataa ctgaagttgt cccttttaaa agaagcgccg ccataccgtc
     1681 tttattaata tcgtctgtta cagtatttgc taatgaatag aattctttaa atgtttcgaa
     1741 cttgttaggt cggtattgat tagtgatccc cttcccacct tcaaattcat aaatatattc
     1801 tacttttggg ttcatgatca cttgatgaca gtattcataa taatgtaagt taaatattcg
     1861 atcctcacaa ctattataag ttttaaatag aatttggtta tttttcagaa tatcgctacg
     1921 atagcatttg ttccatacaa catacatcaa ctgttcattc attaactgat acatgtcttt
     1981 tataaactct tcgtgagtta gatagtgaca ttctgtaact aagttttctt tttcggaaac
     2041 gattcttccc tcgtccattg tacgaaaaac aaacgaacta acaacaagat ccgcttttgg
     2101 actcttttct agcaaatgat cataatctga caagatcgtt tttactggcg tgtcatctgc
     2161 atcgaagaat aagagatatt ctccttttga ctgtcttatt ccttcgtttc ttgctaatcc
     2221 gggcccttga ttggtcgtag taatcaattc aaaacgttca tcattttcaa tcagttcttg
     2281 aatgatttcc tgtgtttgat ctgttgatcc atcatttact aaaacgactt cgtactcttc
     2341 ataatccaat tcttttagaa tatcaatcgt tcgaccaata gttgcttctg cattgtatac
     2401 tggtacgatt atgctgaatt tcggattgtt catttttacg cctcccaact agcgaaagat
     2461 tttttcattc ggaaaagcaa tttctgccaa tgagatcaca ttttcgtctt cttcaagtcc
     2521 ttgagaggac attttatcaa ataggatctt gatggtttgc agcaagatct ttaaatcaag
     2581 catcaatgaa tattttttga tataaagtaa atcaaaacgt aatttactgt tgtaatcaga
     2641 gacatatttt ccatagactt gtgcataccc agtgatacca gcacgaacat tatgccgtaa
     2701 gtagtaataa gggttttctg cattgaactg atcgacgaag aaaggacgtt caggacgtgg
     2761 tcctacgatc gacatatcgc ctttcagcac attgaagatt tgtggtaatt catcaaagcg
     2821 gacagctcga ataaatttac ctacaggcgt tactcgtgca tcattgctct tagctaaaac
     2881 tggtccagat tttgcttcgg ctgtggcggt catcgagcgg aatttataga ttgaaaattc
     2941 cttttgattt tttgtgatcc gcacttgttt atagatgatt ggtcccggtg atgtcacttt
     3001 gactaaaatt gctgtgaata acataaatgg tgaagcaata actaataaaa caagtgaaac
     3061 gatgatatca aacaaacgtt tgaatagttc atattcaggt tggattctaa atccagatgc
     3121 ttcaataata ctttcatctt cgaaattcat aatatttgga ttgatcaatg tcaagttttc
     3181 aaatgttgta ttcaaaaaca attttttttc atttttagta actaattgat agattttgat
     3241 tttttcagtt tcttcgatat gagaagcaag atagacaata tcgatgtctt taatcatatt
     3301 tttgatgttt gcataatagt tatcaagaat gactgcttcg actttatgtt tgttgttttt
     3361 ctttgaacca aaattttgga ttgctggtgc gacatccttt tctaacccaa taatgacgac
     3421 ctttttatct cgacttacgc gtaaataaag tttatatacg agaatacgat agatacttaa
     3481 gaaaagcgta ccgagaacaa atgaaagtaa gataacaaga cgtggaaaag caaaccagcg
     3541 gcccgcaaaa gtcactacca tgatccctag tgtcatcaat acctgaccga tgactgtgac
     3601 aaacacgatg tcactgatca ttcgattata aaaaacatat gcacccaaca aaatgttcaa
     3661 cgcaataaaa atcactgaaa taataattgc tgagtgttgg aaagtttcta aatttcgtaa
     3721 tgggatctct ccacgaaact tcagtaagaa actactatat attgctgcat tgtatacaat
     3781 aacatccatt aatataatga atatccgtcg tgcagcactc cattctccat ttttattcat
     3841 atatacactc ctaatcacaa aataatatct ctattatctt accatagtca cctttaaaaa
     3901 cctagtttat aagcaaattg taatggaatt taaaaatgtc taataaaaag ctttatacac
     3961 ctctaatcaa gacaaggact ctaaattaca aatctccaga tgaatcgatt ttcactaatg
     4021 ttggtaaatt aaagaagtcg aagtatttgc tgcttttttt atccactcat aaagtgaacc
     4081 tttatatttt tcagagaatc catcttccca aagcgctgtc tagtccataa gaatacctgt
     4141 attacgtgaa gaatcgaaaa aattgaaatc gatttttaat gacattatgc ttatagaaga
     4201 aagtgaggta atatcttatg aataaacaag agttgcatga ttttattggt tttcgacaac
     4261 caaatatatg tcaaattgtc gcatataagg atggggtaga agtttataca gatgaatgga
     4321 atggttataa aaaagacgat acttgtcata tcatgtcagc aacgaaaagt attgtatctt
     4381 tgctgattgg cattgcttta gagcaaggat taatcaaaag tatcgatgat aaagtattag
     4441 attattttcc agagtataaa gtgaaacgcg gggagaaaac tatttacgct attacaatca
     4501 agcacttgct aacaatgaaa gcaccctaca aatgtaaagg cgatccatgg acaaaagtct
     4561 gttcaagtga agattggaca aaaacatcac tagatttatt gggaggaaga aaaggtataa
     4621 ccggcgaatt caactatcaa actgtctgtt tacacatatt aactggcttg ttgtatcaga
     4681 caagtaagct aacaactgtt gattttgcca ataagtatct atttgatcct atagggatac
     4741 caaaccatgt caattattat gctgaaacag ctgaagaaca taagcagttt acaatggata
     4801 agtctcctaa aaccaatgtc tggttttgtg atccactaaa tatcgggacg gcaggatatg
     4861 gtttgtgttt atcggcaagt gatctggcaa aaattggtgt attatgccta aataaaggga
     4921 tgtataaagg aaaacaaatc gtaccaccca tgtggataaa cgagatgttc aaacctagaa
     4981 ccagtgcttt agaagactta caaaatatga gatatggcta tttatggtgg gtattagatt
     5041 ctgagaacga aatatattca gcaattggta atagtggaaa tgttctttat attgacgcta
     5101 agaacaatct ggttatctcc atcacttctt attttaaacc catgataatt gatagagtgg
     5161 cttttgtaga ggaatacctt aaaccattta ttttaaaata gcctatatta aaataaaata
     5221 tcgattcttt gacagctagg gttcgtgaaa ctttggctat gattttgtat ctattggcgc
     5281 tctgagcatg aataagagcg tgggacaaaa gtaagaaaca cttttgtctc attctttaaa
     5341 aacgaataaa cggtgggagc agaagcaact tcttcttaaa taagccgaaa ttcacaaaaa
     5401 tttgaaaagc aattttcgtg aatttcttct tatttctcgg agttaaacac ttctgtccca
     5461 acctcgactt atttttttga gtcggcacct tctgcctttt tttgacaaaa aactataaaa
     5521 agccgttatg tgcgagcgaa tcacaacata acagcttttt attatagttt aatctggtct
     5581 caacgaaaag tcgccgattc cttcttttgt taaattatca ttgtaaaatg gatcatgcgc
     5641 aatatatgct ggccatttat cttgcatttt tttgatttca ccagcaaaac gtctttgttt
     5701 ttctggagta tcttcataac ctcttgattt agattcgaaa tggtacaatt ctgcttgatg
     5761 tgcatagaca ttgtaacgac ccagttcata aactttcaag cacaagtcaa catcgttgaa
     5821 tgctacttct aatgtttcat caaacccgtt aacagcattg aagtcagctg ccttgaccat
     5881 catacaagct gcagtgactg ctaagtaatt gacgtcaatg accaatcggc cgaaataacc
     5941 acaatgtgtt cgatcatagt tattcaatgc atgccctgca acaccaccga ttcctaataa
     6001 tactcctgca tgctgcgttg tatcatctgg ataaaacagt tttgctccta cacagccgat
     6061 tcgatcaaac tgtgcataag aaaccatcac cgtcatccaa tctggctcaa tcacttctgt
     6121 atcgttattc aagaataaaa agtatttgcc gtttgctttt tctgctgcta aattgttaat
     6181 tcgagaataa ttgaatggaa tgtcaatcaa ttctacgata aagcggtctt tcagctgatg
     6241 cttgtattct gcaaataatt cttgcatttt tggatctgta cttccgttat ccgcaataat
     6301 gatttcataa tttgaataag acgttttttc gatgatcgaa tccacacaag tttttagatc
     6361 ttcatagcca ttcttcgttg gaatgatcac actgaccaaa tcctcttgca agacgtcata
     6421 agcaatctca taaaatccag aaattctgcc aggacgtacg cttcctttga tgtttcttct
     6481 agacagggca tctgtcaatg ctttcactcc ggaatcatag atataacttt tggcttcgcc
     6541 attacttgca gttgatcctg ggatcgtccg ccaatgatac aagactcggt caatgtggta
     6601 aatatgatct gcagggattt gttctgtcac acgaaggacc aaatcataat cttgagaacc
     6661 ttcatagcct ttacggaaac cgccaagttc tttaacaatg cttgttcgat atacgcctaa
     6721 gtgagagata taattatttc ccatcaatgt atcaggcgac cagtctgctt tgaaatgcgg
     6781 atcaaagcga ttgccatctg catcgatttt atcttcgtca ctgtagatca catccagttc
     6841 tggatgtacg tttagcactt ttgccacttc atataatgca aatggcggta attcatcatc
     6901 attatctaag agtgctataa attctccttc tgctatttcc aatgcagaat tagttgccag
     6961 actgatgtgt ccattttctt ttcggaaaac gactttgatt ctatcatctt ttgcttgata
     7021 ggattccaga cactttcgga tagctggatc agtagaagca tcatcactga tacatagttc
     7081 ccaatgatca tacgtttgat ccagtaccga atcaatgcat ttttcgagcc atttgatttc
     7141 aacgttatag acaggcataa gtatggaaat caatggacgg tacgcaaatc cttgtgcttc
     7201 tttcctttgc tctttcaaat ctggtttctc atgacgactg atccactctg catagtcgat
     7261 cgatcctggc ttcaattcca atttgacccg acggatcgct ttttttacgc catgagtcct
     7321 tgcataattt atcccttttt tcagtaatct tttttttctt tcccatgaag attcaatgcc
     7381 gtcatcataa gggtatttac cgtttaattt cacagctatg ccattttgat gtctcgctgt
     7441 ttggaagtct aagatggctt ttcctctcat tttccctgat aaacgtacct taaaaccgct
     7501 ttgggtctta acatcaagat tatataaatg attgatatcc aatcttacga catgtgttgc
     7561 ttcagcaatc acattttctt tttctacctt tatcgtaggg atttctttgg tcacttcatc
     7621 aattgcccag cccacaataa gcaaatcgcc agtggcccgt tcacgagtga tttgatcgat
     7681 aaatacggca atttcttttt tttgcacgat tcgtttactc cttatcttta taagttaatc
     7741 attatttaca tttcaatgat ccatgattgg ccattttgag ctactgcttc cactaagaat
     7801 gaacgttctt gctcgcaaag gattcgtaac tcaaatcccg cttctgttcc ttttgcgagc
     7861 tgttctgctt gattgacttc ttctcgtgaa acaggaattg cttcgaaaaa tggcgattgg
     7921 tcagctgata acttatagga caagggctgg cgatccgaat tagcgattcc ccatccgatg
     7981 atttttaata tttttgtttc tgcatcccag attttctcgt caatacatat agaaagttct
     8041 ttttcttgga tttttttcga agtttctttc ttacgtcgtc ctagtctatg ttttattgtc
     8101 cattcatcac tttccaacaa tgtgcaatag cgatttgtgg ttgtttttaa cgtatggtct
     8161 agtcttgtat tttcctcaag aagatgatga ttttctcttt ctaattcgtc atttttctca
     8221 gaaagctgct ttgtgacttc agccatggga cgaatcgttt ccaacaattc tttcattgtt
     8281 ggtgtcaatt ccccgataaa gcgatagtga caatggatag tcacttcttt cccagaaata
     8341 tccgttagaa cgaactctgg taattctttc ccatcaaaca aataacaatc atttactgtt
     8401 ttgactacag cattcgaatc gataaatgtt agtttttctc cggcagcttc tgcgctgaat
     8461 tcgataaagc taggttgttg cgcgaagcga aacaccattc tttctgttgt ttctgatacg
     8521 gggaaactta gtgtctcatt ttcccctgtg aaattattga atggtatttg ttggattgtt
     8581 tcatcttgtt tcgtttgctg ggttacttct aacactttta cataattaga attttgtggc
     8641 tctgcaatgg aactttgtgc tactggatgt ttcatcaatg agaaaaagta ttggtagact
     8701 tcaccaaatg gtcgcatttt gaaatcatag cgtacagctt ccggcaattc ttcataagtt
     8761 gattttaatt ctgtatcacc tacagctaag tacaaataat cttcaatatt aatgaataat
     8821 cccgcttttt caaaaacttt ttggaagcca tcatgagtat aaaaagaatt atgggtttca
     8881 tctaggagac cataagaagc ccacggcaat tggttattga acaaatcgat catgacagaa
     8941 ttatgcgcca agttagggaa tgtaatcagg atctctcctt cttcattcaa aaattctctg
     9001 actttcttca atgtttttcc aggatctacc aaatgttcta aaacatccgc aaacaagacg
     9061 taatcaaatg tctgtccagc ataatagttt gcccactcaa aactttcgat atcaccataa
     9121 aatccatctt gagcaaattc gctgacaaaa tcgaacaatt ccttatctaa ctcaacaatg
     9181 ctcacttcac attgttttgc tccgattaaa tgtttcgtta atcggccgtt tccgggacca
     9241 aactctaaaa ctttacttcc tggtttgatc tgtgcggcaa tttttcccac actgctttgt
     9301 tcatctaggt ccatttcaaa atcatatttc attgctatga gtcccttctt cccatttatg
     9361 gtctaagacc acgatgcctt ctccgatata agaaccgctg acaaacaagt tcatgtactt
     9421 tgtcttatat accaaaggaa ccgttgcatt ttcttcaaag accgcaacgt caaaataata
     9481 ttcaccagct aacaaaccta acttttcata ctccagataa aagatattct ctcctttttt
     9541 ccaaggaatc ttgataccat ctagtagagt attaagtccg catacataaa aattgtcgac
     9601 tgttcgtagc gcaattccta aaaccggatt tttgattgta tcatctttta cttcataagt
     9661 tactttaacg tatagttttt ctccttgttc cagcatatcc accggatttt tgtctttatc
     9721 caacaattcc gctttcgtca cgtcgacgat atctggagcg gcgaaccgtt ctacttggtt
     9781 tttattccga tccactgttt taactgattt tttctttaaa aagttttcat aattcgtcgt
     9841 tacttccatc gtatcgccat attcgacgac ttctccgttt ttcaaccaat aggaacgatc
     9901 acaaaaacga cgaatcgaac tgatatcatg agaaacgaat aaaatcgtct ttcccgagtt
     9961 cttgatttct gtaaattttt ccatacattt tagttgaaat tcaagatcgc ctactgccaa
    10021 tgcttcatcc acaatcaaaa tatcgggatt cacattgatt gctacagcaa aagctagacg
    10081 aacgaacatc ccgcttgaat aagtttttac aggttgataa atatggtctc cgatgtcagc
    10141 aaattcaatg atatcatcga ccatttcatc gacttcttct cttgaaaatc ctagaaccat
    10201 tccatttaaa tagatatttt catagccaga atactcagga ttgaatcctg atcctagttc
    10261 tagcaaagct gagatttttc cttcgatttc catcgtgccg gatgttggtg tcagtactcc
    10321 cgtaatgatt ttcaacattg tagattttcc cgatccgttt tctcccacga aaccgatcat
    10381 ctcacctttt tttatttcta cattcacgtt tttcaacgca taaaatacat catgatagga
    10441 tttcttaaaa ggattgaggg cttcacgaaa tcgatccgaa ggttttgcgt acatattata
    10501 agattttgtg atatctttta gtctaattgc atattctgtc atagctattc tccttacagc
    10561 acatctgaaa aatgtggttt caatctacgg aacactgcag agccgattac gaacattact
    10621 aacgtgaaaa cccagaaata caaagtatat gttccatgct cccagaacca agcttcactt
    10681 aaaaatgact cacgataacc ttgaacaaca tagtacagcg gatttaattt caatatgctt
    10741 acaagacctg aatcaaactg attagggctc cataatatcg gtaatgtcca catgatcaac
    10801 tgcagcacga tcgtaattaa ttgcatgata tcaggtaaaa atggttggat cgatgccgtc
    10861 agccaagtca atcctgtcaa gaaagcacac atgcaaaata aataataaat cagctgtaaa
    10921 gacattgctg aaggatatat accatttata agcgcgacag ctatgccaat cagaatgaag
    10981 aataagtggg tgtacagatt ggataggatt ttcgtcgtgg gcaagatacg aatattgaat
    11041 acgactttct ttactaaata agtatattcc cgaaaaacaa ccgtagttga tgcaaatgca
    11101 tctgagaaga agaaccaggg aatcatcccg gtaactaaat atacgataaa tgggtatttt
    11161 gcatcgatca tttgtgcttt cagtccaaca ccaaatacca gccaataaat caatacagta
    11221 aatagcggat tgataaacgc ccataagatt cctaaaaacg atcctgcata tcttgatttg
    11281 aagtcattga tcgaaaactg tagcaataat tttctgttct gataaatgtc cttcataaat
    11341 aaatacaaat ctttaaacat ttgtgttcct ccaagcgatt ttcagtgaat tacagtcaag
    11401 tgccatttta ccatattttt ggtaccgagc tgcgttctta tttctttctt aattttctat
    11461 gatacaagat tttctctaac gtttctattt tacagaagat gctagacaat tgcaataaac
    11521 caaaaaaggc tgcgacggtt gtcacagcct ctctcgtatt ttgtgcctat caatgatagc
    11581 gttggatcct ggaagaatga gcgctatctg gttgttcctc ataagatgta tcatcataag
    11641 tcatatcgtc atatgcctct tccataacat agaattcctc cgcaacttct ggttcatcat
    11701 atcgatgaac ggccatagcc aacaggatat aaataatcgg aacccatggg tatgaagtca
    11761 atgtatcctc cataaatgac tgcatcatca ccgcaaaaaa ggcccagata aaagtcatct
    11821 gcccgttgga taaatctgct ttcaacgtta agcgaccaat aaaaaggata agcagcaaag
    11881 cagcaatccc aaatcctatc aatccataga tattcaaaat agaattataa ctattgtggt
    11941 ttcctaaaaa ttggtttcct cgttggaaga taaatgtttt cattcctagt aaaatttgtt
    12001 cagatttttc tcctagcgtc tgatagaatt tatgccaaat atcttctcga ccggtaaaca
    12061 aattgacttt ttcagagaca gccgccagat aagaaatcgg caaagctaaa agaatcgtaa
    12121 ccgtgtaagc aataaatggc gcacgaatga cacgcatcaa cgatttaggt aaaagatcca
    12181 atatcgcaaa aacaatcaac gcgactaatg cacctctaga ttggcagacc caaatcccga
    12241 gaaaagctgc aacaactgct actgcaccga tgattcgtac ataccatcgt tcaaaagaac
    12301 tggcaaatcc actgatcaat aaagcaagag tcatcaaaga acttccgatt atattcgtat
    12361 tgatccaaat atcagaaagc gaattgcctg gagcccagat gctttgtcct tctggtactt
    12421 ggatctctgt aaatacacga tataacgtga ttccgaggaa gaccgaaaag acaatcagat
    12481 aaataccgcg atcccatttt gtccactcga tccatttaat tactaaacaa aataaaataa
    12541 tcaaaggaaa cagtaagtct ccaaataaag actcatttct taattcccga aagtaagaaa
    12601 agaaaatgaa aagggcaagc gcaacaccta gataaagaaa atcgacgcct tttgctttat
    12661 aaacatttaa aatgagcaaa atagccacac taaaagcgat aagtaatgaa ctattgttat
    12721 atataaacaa agttgctgga aaacctaacc ctgctgtcca aacaaaaaag actagcgtca
    12781 gtaacaaaag caagaaaccg acactcattt tgttgacaaa tttttgtacg attgcttgaa
    12841 ttatctccac tctcctttct actaacattt caggattatt ctcagctagc ctattactgt
    12901 acgactagct gattttcctg agtttgtgtt tgtggtacat ctggataatc cggatattct
    12961 actttaaaaa tcatataacc gtctttatcc ggtccatata gctgcgtgaa ctgcgcttca
    13021 tataattggt tgtaatcagt taatggtcgt ttactgatca catagtcgat ccctaatttt
    13081 tctgcgtctt caaaattcaa cgtgaccgaa aacatatctg gacgatctaa aatgaacttt
    13141 gtatcttcct tagcgataaa agctcttgta tgagcatacc gattataata cttctcgtat
    13201 tcttctttcg gatcgatttt cttccatgca gctatatctg gataaaagcg tactgtattg
    13261 aaggatttca ctcccaatgt tggggtgaag ttatataatt catcttcaga caaccaaact
    13321 ttaccaggat cttgttgatc gattttttgg atttcttttg ccagtgtttt ttcaaaaatc
    13381 gctcctgtac cttctacgac tggattgacg aaaaagccag atacgataac taaggcggca
    13441 aaagcgaggt aaaacaagcg tctccagcca aataatgcaa aagctaaaat cactgttccc
    13501 aacacagcaa ctgcgatggt ttcaaaatcc gaaaaataat tatccatttt cgaatgcgta
    13561 atcgcataga aataagacca tgcatttact gccaacaaaa tgaatttata ccaaatagga
    13621 ataacatctt ctctccaaat ataagcgata aaccagatcg ataataaaca agccgcaaaa
    13681 ctaaaagtaa ggatggctct taatggagga acataagaca gcagagtgat ttctgctagc
    13741 cattttggca acccaacggt aatccaaaag atagaaaaca gacaaaaaat catcaaaata
    13801 cgtcccagta acttttgttc gctatctttt ttgccaaata acacaaatgg acttgctagg
    13861 aatacagttg gaaaaaagtg atagaacagg gccacttcag agttattcga atagttgata
    13921 tcttcaaaag gaattttcca atttgtaagg aaagaaaaaa attcgccaat cgtccacttt
    13981 cctccagtac ttacccgttt tcctggatat aaggtattca aagaagctaa caatgcgtct
    14041 tttgacgtta gccagaaatg aaccatgact ccgccaataa ataaaactgc ccctccaatc
    14101 aacactacat ccaacagatc ccatgaaact ttcttgctat agtaaataag caggccaaca
    14161 aaataaaaaa tcagcaagta cgccagcatc acttgatggg ctgggtaaat cactaaaata
    14221 aacccaatac cgaaaataac caccaaaagc atcatcaacg ttcgtagcca ttttttatca
    14281 tgattgtaga aataatgata aaaagcagtc atcagtccca aagtaaagaa aatcaaatcg
    14341 cccacatgtt gcatgaacca ccactggact gctggagaaa atgtcagcgc aaatgcaccg
    14401 attagcgata gagctttttt tctcttcgtt aatatcatca gtacttcgaa tccaagcaag
    14461 accatgacga ctaatttcat cgcccaataa aaggaaagtc ctcgatctct tccgaggaaa
    14521 aagaatcccc agttgaatgg ttttccgata accgtaatat cctttacagg agaattatac
    14581 gcaatgatca tgttttgtcc gtttaatgaa taatcctcgt tcaccaacgg aaagtcctct
    14641 tcagcttgtg agaggtaaaa aggtgtctgc accagccatt cgtctgaccg cacttcccga
    14701 ttctgtccaa aaatcacatc agtttttttc ccatcgtctc tttgcgacac atatttatcc
    14761 caaacaccaa gtgaacttcc gtttaaatta aaagtaacga tgaccacgat gactaaaacg
    14821 ccaataagat agcggagttt tagaaacagg tcaagcacca aattcgaatc tgcctggttc
    14881 gcttttggct tttcttttcg tcgtgcactt cttgtttgtt tttccatatc atgctccaaa
    14941 ttctatttga taaaaggaaa ttacttattc atttttttgg cagcttccag catttgcgca
    15001 agtgcttctt gccaagttgg tatgacaaat cctaactctt ctgtcttttt caggctcatg
    15061 actgaatact gcggacgtgt tgctttttgc ggaaattctt ctgacgtaac aggtgcaact
    15121 tctacatctg tatctttcaa tatttctttt gcgaattcat accaagaaca gctattttcg
    15181 ttcgacaaat gataaatacc aaatggtgct ttttcttcga tgacatatac cataaattct
    15241 gccaaagtac gtgtccaagt tggtcgccca aactggtcat ttactacagt cagtttgtcg
    15301 cgggtttccg ctaggcgttg catcgtgaag acgaaattgt gtccatagat cccgaaaacc
    15361 caagaagttc gtatgatata atagtcttct aaaatctctt ctactgcttt ttcgcctaat
    15421 aatttcgtac gaccatattc atttagtgga ttcgtttggt cgtctacttc gtattctaca
    15481 tttttgtttt ttccatcgaa aacatagtct gtactgatat agacgaattg agcattcaca
    15541 gcttttgctg caagtgctac atttcttgta ccatcaacat tgatcttctc atctaattct
    15601 tttgcttctt cttctgcttt atctactgct gtgtaagctg cacaatggaa aataacatca
    15661 ggttttaatt ctgtgatgta agccattgtt ttctcggcat ctgtgatatc caagtctttt
    15721 gcatctgttg aaacatattg cagatttttt tcatctaata aatggcgcaa ttctgtacct
    15781 agttgcccat ttcctccagt taataaaaac atatgattat ccgctccttg atttttattt
    15841 taaaaaagag acaggcgagc gcctgcctca catgtgtttt tccattaata cagtgacatt
    15901 ttctctgtat tattgtccat ttttggcata tgctgcttca acggcctctt tttctttttt
    15961 ccaccagtct tcatgttctt cgtaccattt gatcgtgtca gccaacccat cacggaagtt
    16021 cgtaaactca ggcttccatc ctaactcttc ccgtaatttt gtcgaatcga ttgcataacg
    16081 caagtcgtgt cctgcacggt cgtttacatg gtcgtacgca tcttttggtt gacccatcat
    16141 ttctaagatc aattccatca cggtcttatt atcttcttca ccgtctgcac cgatcagata
    16201 ggtttcgcca attctgcctt tcgtcaaaat cagccacact gctgaagaat gatcattcgt
    16261 atggatccag tcacgaacat tctttccttc gccatataat tttggacgaa taccgcttaa
    16321 gatattcgtt acttggcgag gaataaattt ttcaatatgc tgataaggac catagttgtt
    16381 tgaacagttt gaaatggttg cacgtaagcc aaaagaacgc acccatgctt ttactaacag
    16441 atctgaacca gcttttgtag aagaatatgg acttgaagga ttatatggtg tttccgcagt
    16501 gaatttttct ccttcgcctt ccccatgtcc tggcaggtct tcacgtaatg gaagatcgcc
    16561 gtaaacctcg tcagtagata cgtgatgata gcgtacatca tacttgcggc aagcttctaa
    16621 aagtgtatac gtaccaatga tattcgtttg tacaaatggg aaaggatctt ttaatgaatt
    16681 atcgttatgg gattctgccg cgtaatgaac aactgcgtcc gcttctttta ctagacggtc
    16741 cactaattct gcatctgcaa catctcctac gaccaattct acgcgatcgc taggaagtcc
    16801 agccaagttc tctttgtttc ctgcataagt cagtttatcc aaaacagtga catgcacttc
    16861 tggatgattg tttaccacgt aatgaacgaa attagagccg atgaatccgg cacctccggt
    16921 aacaatgatg tttttcataa gaaaatttct ccttaattct tagatttctc cgtaaacaaa
    16981 tggattttct gcttcaaatt cttttaatgt cggatgacgg tgatcttttt ccgacataat
    17041 cgctcttgat ggatcaattg gccaatcaat cgctaaatct ggatcatcaa atgcgatgcc
    17101 cccatctgct tctgcattat agtaattatc acacttataa aggaagttca catttggtgt
    17161 caaagtaacg aatccatggg catatccttt aggtacaagc aactgtcgat gattatgttc
    17221 agataagata aatccgtccc attgaccata agtcgggcta cctttgcgga tatccactac
    17281 tacatctaaa actgctccag tgactacccg aatcaatttt gtctgtgcag cttctccttt
    17341 ttggaaatga agcccccgaa ggacaccagc ttcagctgat agtgaatggt tgtcttgaac
    17401 aaagtcatat ttcaatccat gttctgcaaa ttttgctttt gaataacttt ctgtgaagaa
    17461 accgcgatga tcgccaaaga cgtccatttc tacgattttg acatctgtta attttgtatc
    17521 tgttactttc atctgtaagg actccttatt cagcggccaa attcaataaa tattgaccgt
    17581 attgattttt tttcaatggc tgtgcaaggg ccagcaattg ttccttatcg atgtagccca
    17641 tacggtaagc aatttcttcc aaacaagcca cttttaagtt ctgacgtttt tcaattgttt
    17701 cgataaaggt agatgcttca agcaatgatt catgtgttcc tgtatcaagc caagcaaatc
    17761 cgcgtcccat aagttctacg gatagttttc ctttatccaa atatgcttta ttgacatccg
    17821 taatctctag ttctccacgt tctgaaggtt tgataccttt agcgatagaa attacctcat
    17881 tgtcataaaa ataaaggcca gttacagcgt aatttgattt tggctgagca ggcttttctt
    17941 caatcgacaa agcttgcata ttctcatcaa attctactac accaaagcgt tctggatcat
    18001 tgacatgata gccaaatact gtcgcgcctg tatcttttga agctgctcgt tgcaacattt
    18061 tagacagacc gccaccatag taaatattgt cccctaatac caagcagaca ctatcatctc
    18121 caataaattc ttctccaatg ataaacgctt gtgccaatcc atctggactt tcttgtactg
    18181 catattcgat ctgcagtcct aaatcatttc cgtttccaaa taattccttg aaacgtggtg
    18241 tatcttgtgg cgttgaaata atcagaattt ctttgattcc cgccaacatc agagtagaca
    18301 ttggataata aatcattggt ttgtcataaa tcggcatcaa ttgtttcgat gttgcctttg
    18361 ttaaagggta cagacgtgtt ccgctgcccc cagcaagaat gattcccttc atggatatct
    18421 gctctccttt ttgatttcaa atttcacttt gttattatct catcaattgt tatatttgtc
    18481 atggaaatat taaggattta aacatctttt atcaacgttt aaaccatttt cgtttgatca
    18541 acgccgtaat cagatgaaca cgccaagaaa ttttttgccg taatttagaa tgtgtttcaa
    18601 tctcactcgc gtttaaatca tgtcttcggt acagagttaa cggttctctt aggaacccag
    18661 tcttctttcc tcccagcaaa ccaatccaca tatcatgcat cggcacttct gacgggatcg
    18721 gcaaaatatc gggttttaaa gaagaccgga aagccatacc agcaccaata tagtgacttc
    18781 ttatcacatt attaagccat ccttctttgg aatttcgaaa cgaaaaatac gaatcatgga
    18841 tctcttgcag ctcgctgtcc acaatgatta aatcagaaac tactactagc tgatccggat
    18901 tctgctcaaa atagtcagtg atctttttca cttttgtctc caaccaaaca tcatcttggt
    18961 ctgccaaaaa gattagatct cctttcgtct gctggatcgc ataaccaaaa ttagcgatca
    19021 atccttgacg aggccccgaa aaaagctgga ttcgcttatc tttttctgcg tattcagtta
    19081 atatatccca tgtatcatct actgacccat catcagaaat gactacttca tcttctgctg
    19141 aaagctgaga taaaatacta tctaactgct ctcttaaata tctgcctcca ttaaaggtag
    19201 ctatacaaac cgaacgcacg aattcacacc ttccaattct taacatatga gcgcaaagtc
    19261 atccgccaga tatgccggtt tttgacggtc aagaactgtt tagctgttct taacaataat
    19321 tgtcgtcggt gactggataa atgcctttga tcatattcat gataaaatcg attttctgct
    19381 tgtaaaatag aacggtatcg attacttgaa actttttcat agtctaaaac cgaaagatca
    19441 tgttccattc gtacaggaag gatctccaca gttttttcca actgatccac tttccaaaac
    19501 agccaatgat ccaaaaaatc taaagaaaaa gcaggattaa acccgccaat cttttttaga
    19561 aatttgacag accaggcaac acctgaattg attgccatga ttcgttcagc tgttgggcct
    19621 tcaacttcta ctgcttgagc tgtacgattg atataacgat ctgaatatag gggagaaact
    19681 tgtcggctcc cagaaaaaat catcggaact gcagcaacac attcatctgt aaaggttact
    19741 ttacgcaata gttcaaaata atcgtcgact agcctcgtat cctgatccag agtgacgaga
    19801 caacgtgttc cttcatctgc ttgagacaac gcaaaattat aagccaccgc taaaccagga
    19861 ttttgcggat catgatgata aacgatattt tctttttcaa acagctcatt ttgctgcctt
    19921 tgaggactat tatcgtaaat aataagctga atttgctttt cgctaagtgc tttgcttaat
    19981 ggtccataag ctggagattg actgaatagt tgctgataaa gcacaacgac tactaatatt
    20041 tctggtttca tcagaaaaaa cgccaccccc acttattgaa aaatttcatc atagaagtca
    20101 aaaacacgcg aaacagtttt gcatttcgat gcgcaccctt cccgtaaaaa tggataactg
    20161 cataaaaggg tgtgtacaat aacttgtaac ctgctttttc aaaactcaaa cacaagtcgt
    20221 tgtcttcaaa gtacatgaag aaacgctcat caaacccctt tatctcttta aatttatcaa
    20281 tatcagctac cataaaacta cctgagatca tccgtacata actattagca tcagtaggca
    20341 atcctcggca ttcaaacaca gccagtcgtt tgtcaaactt ttttttgacc catttgaaag
    20401 gcacaaagcg caaaacgtaa tcaaaaacag ccagctgctc acggatgagg tgctgagttg
    20461 ttccatcttc attaagaatc ttaggtgcta acatcgcaca ttccggatgt tctttcaata
    20521 acaagaccaa tgctgtaact gtctcttcat ccactaaaat atctgggtta caaataatgc
    20581 catagcgaga atcgctagct atcagattgt gattatgtcc ataaccaaat ccggaatttt
    20641 catcatgaaa atagatatcg atgtagggat atttcctata ctctgttagt ctttttttgt
    20701 actcgggaag tgaattgttg tcaaaaattc ggatttttac tgctgtttca tcttttacag
    20761 ttgaaataaa attatctagt gtccgaaaaa ttttatcact gtttgccgtc acaatactaa
    20821 taaggatcgg ctggtttaat ttgttattca tctatatgac ggttccattt cttcaaatat
    20881 ttctgtttta cttgcgtacg gtactcttta ttccctaaaa atcgcaatgt ttttaaaata
    20941 aactgatgtt tctcattgat cagaccaatc aattcaaata ataaaattgc agcaaacaac
    21001 atggcaataa tcaatatgat cgttccgatt cttccagtat aattcatgac catagctgtg
    21061 aaagaaaaca tcaaagccat cgcataaatc gtcaggacag cacctttatg agtaaagcct
    21121 aaactgatta gtcggtgatg aagatgtttt ttatctgctg tagaaaaaga aacacggttt
    21181 gcttttctcc taatgatagc aaaaaacgtg tctgttaccg gcactcccat gacgatcagc
    21241 aaagaaatcg atgaaacaaa agtcacgttc ttcagccctt gtagtgaaag gacggccatc
    21301 ataaacccta ggaatagagc tcctgtatcg cctaaataaa tctttgccgg ataaaaattg
    21361 taaggaaaaa aaccgataat acttgctaca agacagaaaa taacaactgg aatatacact
    21421 gtagaagcat ggaggaaaaa atatccaatg attcctatcg taattaaacc gatcaatgaa
    21481 atccctgccg ccagcccatc caaaccgtca atcagattga ccgcattcgt gataccgaag
    21541 atccaaaaaa tcgttacggg aaaactaagc cagcctagtt ggatatatcc aataaaagga
    21601 agcgtgaagg aatcaatacg tatatctgct acaaaataaa taataagaga agcaatcaag
    21661 atccctatac ttttttgttt aggtgtcagc tcatatttat catcgagcac tcctgtgact
    21721 atgattacta aagaagcaat caagatcggt acaatatacg attgagggat aattttacgg
    21781 aataagaata aagtcgatat aacaaatgaa ataaagatag gcagtccgcc cgctgaaggc
    21841 attggaactt tatttattct tctttcattt ggtttgtcga ccatacctgt ttgtactgaa
    21901 atgattttga atacaggtgt taatatcagt gataataaaa ttgtcagaaa aaatcttata
    21961 accatactaa aagaaacaag catatgggca cctactttaa ttaagagtca taattattat
    22021 aaattatata tgtctttttg actatctttt tatatcttct caaatgtccg caaagcctta
    22081 ttttatcggc tctacgggca ttttgctttt gtggtaaacc tcgcatatct aggtctatct
    22141 tcttatattt tccctgtcaa tcgtggttaa aatcgtggta aatacttcta tgcgattaga
    22201 cgctgaacct ctgattttgc ggtatctatg gacgcatgag cgtaccagtt cattgtgata
    22261 ctgatgtttg aatgccccat gatatactgt aaatctttcg ggttcatgtt cttgcttgcc
    22321 agccttgtgc agaatgtatg gcgtagcgta tgcggtgtaa tatgtggcaa gggattgtcc
    22381 ttgtggtgct tgttgtattt ctttaccata cggataaata aggcgttata atcaatggca
    22441 acttttggat tacctttcgg attgataaac acaaaaccgc tgtgtccgtc tacctctatc
    22501 gactttatct ttggtcggct gttgataacc cgttgaaatg cctgtatcgt ttctttgctt
    22561 aatggcactt gccttatccc gcttttcgtc ttaggcgttt caatataata gccctgttcc
    22621 ttactcttta gtaactggtg gtcgataatc acaacttcat tcttgaaatc aatgtcggct
    22681 actgtcagtc cgcacagttc cgagatacga agtcctgtct ttaacagtat cagcacatca
    22741 tcataatgct tgtgatacac gttgtccgtc ttaataaatg acaataaggc ttgctcctgt
    22801 tcctctgtca atgcgacttt ctctttggta tcattttcta tgacttcact tagcttgaaa
    22861 tcaaaagggt ttttcctcac ataatcatct tgtatggcta tgtagaatga cgcttttaac
    22921 gagcgtttat ggttgttaat ggtattgtag gaaaagcctt tgtctttcat gcgtaacgcc
    22981 cattctttag cgtcagaggg ttttatcgta tcaatgctcc tagcacctaa cttgtcctct
    23041 tttaataacc gcatgagttg ttcccgttgt ttctgtgtgc ttttcttcac atttgtcctc
    23101 tgtgcgttct gtttggcata gagttggcaa agcgtcattt tcttgcctgt gctgtcgata
    23161 ccgtcctcaa tatcccgtct tatctgctgt tccagttctc gaagtgaggg tttttcccgt
    23221 tttccctttg gtgtcgggtc tgtgggtgtc aatctccaag catacacata ttttgtgttt
    23281 cccaatgcgt ctacatattt atataagtat tttccgtctg ttcgttggct ctctccagta
    23341 tgcaggatac gacctttgtt atcccgtctt ttttctttca tggtgtctgc tcctttcctt
    23401 gttggaaaga gccttgatat gacttgtgtt catcataaca catacaaggc tcatttgcat
    23461 tagattgcgt ccaatttatc aataaccttt tcaaactgtc ggcgtttaat ctgtatgcgg
    23521 ttgccattca taatgagcca gccagcgtcc ttgttttcct ctgccaatcg tcttaacttg
    23581 ttttctccaa tgcggaaata ctttgacgct tcttcaatgg taagggtgta tttttcccat
    23641 acgggaatat cctgtgtatt actcataaaa caccccctca ctttcgatta cgtctgatat
    23701 gtccgtacac atctgttcca caagcatttc ctgtttttca gaaaggtggg tacattcatc
    23761 aatcattttc atgtagtttg aacctgtcag cctgtcgatt tcctctatca tcacgattga
    23821 cggcatgacc tgtctttcca gccattgcag agtgcgttcc atattgacag gtacagggtt
    23881 catttcaaaa ggtaaactgt catgggaaat gaatatctcc cacaatggat agtcaggaaa
    23941 ttccacaaaa tcggtaatga gataaaacac cagcccatga gggttatacg tcaataacag
    24001 ttcttcaact gcctgtaccg cctttttgtc acgcagacga atttcaaaac ggttgataat
    24061 atcggtatgt ttctttttcg ttgcctgttc cttttgtttt tcatacaggc agaaatattt
    24121 tgtgcttgcc tttgagccga tataaagggt acttgctaaa ttacggtttt ttccgcttaa
    24181 ttttccacct tgtacatttt catagttttt acagcgacat tccgcaccac catttctgta
    24241 tttttcagat agcacaggaa tatccagcag accgcacata tcattgattg ccaagtcaaa
    24301 acgtcggata acaccgccac agtcaagaca gcggtttaag aatgaatacc agtccctgtt
    24361 ctgtgcctgt aagacatatt ccatgttgcg tgagcctgcc ccttttaatt ctaaaaatac
    24421 gcccatgtgt tcatcatcag aagccatgac cttgatttcc ccgtaagcat aatgctcctt
    24481 atagccataa tacccatagt cataatggat aaaataatct gctttcatgc caagcacctt
    24541 tcggataact tccaatgcgt cagtcgttgg aaaacggaca gacagataat caacgagcag
    24601 ataaaaaggc ttatcgcaga tatagtaatt cagacaacgc aggataatgt tctgtgtttc
    24661 cttgctggcg ttagcttttc cgttctcaaa gcggttgata ctatatcttg atacatgggt
    24721 aagacttgca agttctcctt gtgaaagacc gctggcaaga cgtttgttct tcatttcaag
    24781 tgcaaattct aaattgttca ttgtattcct ccaatcccac aatagccttg aaatacctgt
    24841 aaaccacctt tcaaaaagtg tgatatgtac tatcttctta aaacctctca ttactactca
    24901 caggtttggg ggtgtatggt ttttgacttg tcttttgtga aatagtgccc ccctgttaga
    24961 taacgggggc acagacaagg ctcgcaggct cgccagccga caaagcattt cttccccttg
    25021 acacaaggtg tcatggcggt aagcctgaga ggcttccgcc accgtgtcag aaatactttg
    25081 tcagcaagtt ctgttttgcc ttttctaaca agttccattt caaccgatat tcatatctac
    25141 ggatttaatc agataggtat ttctttttga atgtttttat atttcattct attgtgctta
    25201 ttcgattaca agaacatagt aattcttata tgcttatttg tcaatatctt tttttgcctt
    25261 tacaaatcat attaagcatg atagacttat aatagcgaag atataacgag atatggaggg
    25321 ttatgcaatg aaaataagcc atttaggaaa taatatacag accataagaa aatttagggg
    25381 aatgaaacaa caggaacttg cggacaaaat cggtatcaat atgcagagcc tttccaagat
    25441 tgaaagaggg ttgaactatc ctgcctatga aacgctggaa aagataatgg aagtgctgga
    25501 cgttacgccc aatgaattgt tgtcgggaga atggaagtat gtcaatcaat ctgaaaagga
    25561 agtctgtcag tttttaagag ccgaagaacg tctaaatgca gaactgaaac atggacacta
    25621 tgataacttc tttgacagcg aggaagaatg gctggaatat gagttagaaa agttacggga
    25681 atatattaca gactatatca acgggaaaag cattgaagcg tccgaccttt acccaatcaa
    25741 agaatttatc cagcatttga agtttcaaaa attattagac cgctatgatg atttatacag
    25801 tatggatatg tttggggaaa gcatagaggg acataagtat aagacacctt atcaagtcgt
    25861 gaaaatgata aacccgaact caaaagaaga tatggaactg ttacgagaag tattaaggaa
    25921 taacaatttt gatgatgagg acgagtaact tttttcctct tgcgagaaga tacagcaaac
    25981 aaaaagcccg atcaagagtg caaagcacca ccaatggtgg caaagctctt gacagggctt
    26041 tttcttttct gttgtgggca atcaagagga agaaagggat aaaatctgat ttgtggtaaa
    26101 tcaggcaaag gcaacgcagg tatcacagta tgaattgaca ccgcataagg aaagcaactg
    26161 gaagattgta gaataaatga aaaaggcttg cattctgcat ttttataaaa ttgcaagtct
    26221 ttatattgac aagcataaaa tactaagata tactttaatc agaccaacgg tttgtattgg
    26281 aggtaagtta tggcacgaaa taaatatcca gaagaaacag taaaactcat tttagatgtt
    26341 tctacaaaaa tgttttgtga aaaaggatat gataacacat cattgcaaga tattatcaat
    26401 gaaacaaaac tttctaaagg tgctatttat catcatttcg attcaaagga agatatttta
    26461 aaagcaattt ttcaaagaat tggaaatgaa aatgcagtaa tatttgctaa aattagagat
    26521 aacgatagtt taaacggttt agaaaagtta agagaaatat ttaagacagc ggtattcaat
    26581 tctaaccaaa gcgttttatt gacagtttct cctcaactat taaacaatcc tcgttttctt
    26641 gctatgcaaa tagaacaaat gtatgaaatt gtagtacctg aatttgttga acccgtttta
    26701 gaacaaggta tcaaggataa gtctttggag attgaaaatt cacatgaaat tgcagaagtt
    26761 atcatggtat taagtaatgt atggctcaac ccattagttg aaatgacgga taaagagggt
    26821 atggaaaaac gttgcaatac attcaatgcc cttttaaatg gaataggcat agacacatta
    26881 ttagacgaag aaacaatttc tgcatttata cagttttgta aataactgta tttgttatcc
    26941 ctatacgata gactgctcta taaaagagca gtttatttaa accattatat aaaccgacgg
    27001 tctgtttgat taaataaaaa ggagagattt tatgcaagca gaaaaaaaac tttttaatcg
    27061 tgattttata cttgttgtta taggacagat aatttcttta tttggaaacg ctatcctacg
    27121 atttgcatta ccgtactatt tgttaaaaga aactggttca tcagctttgt ttggtattgc
    27181 tacagcctgc tcttttttac ctatggttat tttgtcgctg gtaggtggta ttattgctga
    27241 caggacaaat aaaagaaaca ttatggtcat actagatttt gtaacagcaa tcataatttt
    27301 agtattttat ttgtcattgg gaaaaattcc tatcatacct ttgtttattg ttgctttgat
    27361 gttactttat ggtatttctg gtgcatatca accagcggta caagcaagta ttcctctatt
    27421 ggtatcatca gataaactta ccgtcggtaa tgctataata aatcaagtaa atactttagc
    27481 aaactttatc ggaccagcta taagcggagt agtttttcaa ttttatggta tcaatcccat
    27541 tttaattgtt agtattatat gttttacttt ttcagccatt atggaaattt ttatccatat
    27601 tccacaccaa aaaagacctc gtgaagtagg tgtaattgca gttgctaaaa acgatttaaa
    27661 agatagttat acctttttga aaaaggagaa acccattttt atatccgtaa tctttttggt
    27721 ttgcattttc aatttggttt taagtgctgt tttaattgtt ggtctgccta tcttaatttc
    27781 tgaagtttta aaaatgccag atacactata tggtttttca caatcagcat tatcattagg
    27841 tggtttatgt ggtggtattt taacagctct tgttgcttca aagttaaaac taaaaaacct
    27901 ttataccttg ttacttattt gttctgtttc tgttttactt atgggaattt cgctattttt
    27961 agaattgcct gcaatagttt cctactgggt agttacagtg atgtgctttg tggctatggg
    28021 tgtatctact atgtttgttg ttcaaattta cactcttgtt cagacacaaa caccaccgca
    28081 attagtagga aagataatgg ctacgctggt agcagttgct atgtgcggtc aacctattgg
    28141 acaaatggtt tacggtattt tattcgatgt atttatacaa gaaacatggg ttgttatgtt
    28201 tgtagcttct attgcttcaa tcatgatttc aatatattca aaaagaattt ttcaaaagtt
    28261 agagatataa tacaaggggt taaatagtat gttaggctaa aaacaatata taaaagaaaa
    28321 tttgaaatct gctaaaagga tagtgttata aaagaaagag taatattgca gtatttaagg
    28381 ctcgcacaaa tatatgtttt gtgtgggctt ttttcttacc atttttctgt atttctaaag
    28441 ggtataaacg gtattccgtt tatatatttc agcattagca gaaagggggt gagattatga
    28501 ggacatcttc tttcgagcat atcgtcagat tacagttcaa ttctttaatg ctgattgtca
    28561 ttaagaacaa agtgaaaagt cgaaacagac agcttgcaag acattctaaa cgtgaagtat
    28621 tgttttgtga aatagccgaa acgaaacaag ttgaacgtgg aacgattgat acctgttttt
    28681 atgattgtgt atcatttaaa gtcttgcact ttacaattta tgtatctgat gaaacacttg
    28741 gtacagtttt acatagactg tcagaaaaac agcgtagtgc gattttatta cgttattttc
    28801 aaggaatgaa cgacagagaa atttcagaac tgtaccatgt atcacgttct gctatttgca
    28861 gtagaagaac tagaggacta aggaagttaa aaatgctttt gagcgaaagg aagtaaagaa
    28921 atgagtaaag attatggcta tccctctttt gaattgattt gcaaagcgtc aagcggagat
    28981 gaaatggcag ttaaagagat tttgaagttt tatgacggtt atatttctaa attatgcttg
    29041 cgtccttttt accactctga aagtggtaaa atcattatgc aggttgatga agaactaaaa
    29101 ggagaaattc atacagacat gatgaaagca atattgaaat ttgaaatcgg agtgaagtaa
    29161 tcaactgtat aaaaaaatcg ggagatacac agtttcgtgc tatctccctt agtagtttta
    29221 cggtcatatc aaggctcatt caatcgtggt aaattcgtgg taagatgagt gtttttctat
    29281 acttcaaaat g
//
LOCUS       DAYYAR010000022        26251 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000022 DAYYAR010000000
VERSION     DAYYAR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.022"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..976)
                     /locus_tag="ACHAT0_000859"
     CDS             complement(32..976)
                     /locus_tag="ACHAT0_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8450821.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            1197..2411
                     /locus_tag="ACHAT0_000860"
     CDS             1197..2411
                     /locus_tag="ACHAT0_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8450822.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            2433..4448
                     /locus_tag="ACHAT0_000861"
     CDS             2433..4448
                     /locus_tag="ACHAT0_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8450823.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4531..5904)
                     /locus_tag="ACHAT0_000862"
     CDS             complement(4531..5904)
                     /locus_tag="ACHAT0_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8450824.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(5930..6214)
                     /locus_tag="ACHAT0_000863"
     CDS             complement(5930..6214)
                     /locus_tag="ACHAT0_000863"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8450825.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(6216..8231)
                     /locus_tag="ACHAT0_000864"
     CDS             complement(6216..8231)
                     /locus_tag="ACHAT0_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8450826.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8410..8715)
                     /locus_tag="ACHAT0_000865"
     CDS             complement(8410..8715)
                     /locus_tag="ACHAT0_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8450827.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(8804..9835)
                     /locus_tag="ACHAT0_000866"
     CDS             complement(8804..9835)
                     /locus_tag="ACHAT0_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8450828.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10042..10917
                     /locus_tag="ACHAT0_000867"
     CDS             10042..10917
                     /locus_tag="ACHAT0_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8450829.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(11069..11911)
                     /locus_tag="ACHAT0_000868"
     CDS             complement(11069..11911)
                     /locus_tag="ACHAT0_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8450830.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(12003..13880)
                     /locus_tag="ACHAT0_000869"
     CDS             complement(12003..13880)
                     /locus_tag="ACHAT0_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8450831.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(13877..15298)
                     /locus_tag="ACHAT0_000870"
     CDS             complement(13877..15298)
                     /locus_tag="ACHAT0_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8450832.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(15301..18690)
                     /locus_tag="ACHAT0_000871"
     CDS             complement(15301..18690)
                     /locus_tag="ACHAT0_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8450833.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            18947..20338
                     /locus_tag="ACHAT0_000872"
     CDS             18947..20338
                     /locus_tag="ACHAT0_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8450834.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(20411..21058)
                     /locus_tag="ACHAT0_000873"
     CDS             complement(20411..21058)
                     /locus_tag="ACHAT0_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8450835.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(21066..21752)
                     /locus_tag="ACHAT0_000874"
     CDS             complement(21066..21752)
                     /locus_tag="ACHAT0_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450836.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(21758..22831)
                     /locus_tag="ACHAT0_000875"
     CDS             complement(21758..22831)
                     /locus_tag="ACHAT0_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8450837.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            23053..23727
                     /locus_tag="ACHAT0_000876"
     CDS             23053..23727
                     /locus_tag="ACHAT0_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8450838.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            23732..25072
                     /locus_tag="ACHAT0_000877"
     CDS             23732..25072
                     /locus_tag="ACHAT0_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8450839.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(25221..26207)
                     /locus_tag="ACHAT0_000878"
     CDS             complement(25221..26207)
                     /locus_tag="ACHAT0_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8450840.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7621 a         4951 c         4819 g         8860 t
ORIGIN      
        1 ccttcatatg ctttagtgaa agattttctt cttaatgttt atttctccat tcattgggag
       61 tacatgttga atacttcttg aataatttga ataaagaagt cgtttgttta tatccgatac
      121 gattagcgat ttcttccaac gaaaaactcg tttcttgaat cagttccaac gcaaagtcga
      181 attttttctt tagaaccagt tcttgaaagg aagcatcaaa atacattttc aatttattac
      241 taagatagac cggattgtag ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa
      301 aatgcttatc gatataatct aatactctaa gcatctcatt ttctgcgcat ccatctgctg
      361 atgaaaagct ttgttgttct aataaatgag tcaattcgat cagtaatagt gatgtataga
      421 gatttaacga acggatctta tgactgtatg tatcaaaata aatcaacagc atattttcaa
      481 tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc
      541 ctttttcttt tgatgcattt atcaaaaaag aagaaagtag attatttgtg cttgccaaat
      601 gattcaatat ctctgtagat aaggtttctt cttttaataa gatgttgatt aaaatatcct
      661 cttgccctgt gtattcgatt ctttgagtag cttctttatc aatgatcacc acgtcacttt
      721 cttccatctc tatcctttct ccattaataa tttgtaaaca tgtaccgcta tacatataat
      781 tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc atatcgacgg tgtttactga
      841 tatgaatatt tccctgttga aatagatccc ctcggtggat ttttggcggt gtctgaagat
      901 ggtaagctaa gtttaaatca ttgatattca ttccttgttc acgctgcaga gtttcgattg
      961 attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt ccaattgact ccctcctttt
     1021 tgtaagcggt ttctttcgct tattagttca cgctgtactg ctattatatg caaagctatt
     1081 tttggtttca taaagacttt ctatggttgg gacatgaact tcctgtgatt ccgctattga
     1141 gagaataacc acttcatact atatttttga taagcgctta caaaaatgaa aggaacatgc
     1201 ttatgaataa aaagatgatg aaattttctc tgctgtcagt ctctcttatt ttgacttctg
     1261 ctggtgcagt cacgtctgca attcccagta tgtcagctac ttatgccacg cgttctttat
     1321 ctgctgttga attattaacg actgctccct cttttatggt cactattttt gttttgatta
     1381 gtagttttat tgctaggaaa atcggacaaa aacaaactgt gatcatcgga ttagtgattg
     1441 catcgatttg tggtatcctc cccctcttct cacaaagtta cagtattgtc ttagcttcaa
     1501 gagctggact aggtattggc tttggattga tcaattcttt ggcagtcagc atgattgctg
     1561 ccttttatca cggagatgaa cgagcctcta tgattggttt tcaaagtgca tttcaaggat
     1621 ttggtgcagc tttaatgaca tttgttgcag gacagctagt acagttcggt tggcaatata
     1681 cctttttagt ctacgccatc agcttaccta ttcttgtctt gtttgttttg tttgttccta
     1741 cacctgaaac aactgaactt caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa
     1801 cgaatcctaa aatgattagt tatggtattt ttctcttgct ggtcatcgtg gtatataatg
     1861 ctgtatcgat taaattagcc acagtcctga cgacttattc tctaggaaac gaaactaatg
     1921 cagcgaatat cttaacaatc atgcaattgg caagtatggc tgccggagtt tgttttggct
     1981 ggcttcagac aaaagcacat cgctatatgc ttacactttc tttactactg atgggaatcg
     2041 gctttgtatt gattgccact gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa
     2101 caggaatttc tttttcgtta ttcattcctt acttattcaa ccaagtaagt atccaagcgc
     2161 ccaagtacgc ggagagtttc aatacttcaa tcttattggt tggcgctaat ttaggtactt
     2221 tcattgcacc atatggtttg ctctttctct cttttgcttc tcttgggagt aaaacacttg
     2281 ctgttttcgt caatggcagt atcttgctgt tgattagcgc gcttattagc cttctagtag
     2341 tacacaggac gaatagaaag acagtgataa gagtagatga agaaataaaa gaacaattga
     2401 caacaaaata aatttgatgg aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat
     2461 ggctttggta tcctggagac tttgagatcc gccatggact gctacaaaat ttccaacggg
     2521 aagaacgagg ttttgactgg ccagcgtact ggtacatgga tgattgccat cggaacgtca
     2581 agtttaagag atattacttt cttgatcaac cttccatgtt taaagtgacg atccaaggag
     2641 ttgggtatgt agaaattaat ggacaaaaac atccatgtgg aaaatggctg acttgtccag
     2701 ctggtaaagc aaaaattcgt atctttgtag gacatacatc agggctacca gcgatgttta
     2761 tcgagggaga cgaagtaaaa tcagatattg gctggacagc aagtaatttt attgaagaat
     2821 atccagctgg ctggagtccg ctttatgtcg atatcgcaaa agatccaaat caaatctatt
     2881 atcaaaaaga atgcgtccat ccagtcactg aaacggagat tggcggagga atcttattcg
     2941 attttgggcg agcagtgaat ggctgtgtag aactaatatc tctggatgac catatcgaat
     3001 ctatcactct atgttatgga gagtccgatg tagaagctat ggatatcgat tattgctact
     3061 ataagcaaat aggaatgaca agaaaaatga aagctcgtaa acgcgctttt cgctatttgt
     3121 ttataccaga ggtttctact ggaatggttc aacttcaagt ttttcatgaa tttttatctc
     3181 tttcgaaaca agctgctttt tcaagcgatt cttctttact gaatcagatc tggtcagtat
     3241 cagaagaagc cttcaagtta tgtagtgatt tgttttttat cgacggtatc aaacgagatc
     3301 gctggatttg gtctggagat gcgtatcaat cattcatgat caatcaatat cttttttttg
     3361 acgaggaaat caataaacga acgatattag cattaagagg acaaaatgaa atcaagcagc
     3421 atatgaatac tatcgttgac tattcgatct tgtggttgat tggtatcgaa aaccactata
     3481 tgatgacgaa agactatgaa tttttacgca tcgtctaccc taagatgcaa tcattaatga
     3541 gatacttgat ggagcaaacg aatgaattag ggtttattta tgggagagca aaagattgga
     3601 tatttattga ttggtctgaa atggataaag aaggaacgct tgctgcagaa caagtcttat
     3661 tactgaaagc ctatcattcc atgatcactt gcggagatat cttagctgaa acatatggag
     3721 agaacgaaaa ttatcatact catctctatc aagaaaaata tcaactattg aaacaaaacc
     3781 tatttgacta cttttgggat gaagaacttg gcgcttttat cgattgctat gaatctggga
     3841 gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt attcgatttt gctaacaaag
     3901 accaacagca atcgatctta aagaatgtct tattgaatga cactatcaca cagattacta
     3961 ctccatattt caaattctat gaacaagatg ctttatgcaa actcggacag acgaatatcg
     4021 tctaccaaac gattcttgat tattggggag ggatgttatc tcatgatgcg gtaactttct
     4081 gggaagaata cgaccctact caaacaggaa atgaaaagta cagtatgtat ggagatcctt
     4141 atggaaaaag cttatgccat gcatggggag caagcccaat ctatctttta ggacgctatt
     4201 ttatcggttt gtatccaaca aagccaggtt atgaagaatt tatgatacaa cctaatctag
     4261 attcatttga aacactgaat tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa
     4321 aaacaaaaga taagatcgtg attacttctg atagaacagg cggaacagtg aaaatgaatg
     4381 gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt aacaagctat tcaaaaataa
     4441 atgcataaaa caaaaaactg cttgcgacat atcagaagac tttaaatctt cttctgatat
     4501 tgtccaagca atttttattt tgaagtttgc ttaattctgt tctttagaaa caatgtttgt
     4561 tctttttccg gcaaaaagaa agctagataa tacgacaagt aaaaagaaag cgatgattcc
     4621 aactgtcaag gctatttgac cgtattctcc tagtttccca agtacaagtc ctggtactag
     4681 ataatcagta tcggcaaatg tagaagaagc gattttaaga tctcctaata ctggaaggat
     4741 aaataatggc agccaactaa tcaataagct attgaaagca gaacctaata ccgcccctct
     4801 tctaccgcca gtcgagttgc cgaagacacc agaagcagct ccgcagaaga aatgtcctac
     4861 taccccagga atgataactg tagtttgcaa gcctatcata gcaaacatgg atagtgttcc
     4921 tacaaagaag ctaacaaaaa agccaattaa tacagcatta ggtgcatatg gaaaaacgat
     4981 aggaacatct aatgctggtt tagaatcggg tacgagtttt ttagcaatcc cttggaatgc
     5041 tggaacgatt tctgccaaca ccattcgaac cccttgaagg acgataacaa acccagctgt
     5101 gaatgttcca gcttggatca gtgcaaagat tacagcgttg tctccattgc ttagttctct
     5161 ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca tatacgatcg acatcaagag
     5221 catgatcgat acagtagaat ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc
     5281 tgtagaacgg tctttgtttc caaaaaactt tccgatccat ccactagaag cataaccgat
     5341 atttccggta tgccctaacg caacttgatt gtttccagtg atgttcttca taaacggttg
     5401 acaaagagca ggcgtaatag ttaatagtgt tccttcgaag accccgccta ataagatcat
     5461 ccacaaattt gcagtaaacc ccgcgcttac taaaatgact gctgttagac aagaaacata
     5521 aagcattgct tgacctgtaa gaaaaatgaa tttatatctt gtgaatctag caagtaaaat
     5581 attcacgatc atccctacta gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc
     5641 aatcgccact actgcttcat tgctaggaac tactccttga gtatgcagag ctgtttggaa
     5701 catcgaagca aaaggggtta gtgaattact gattaaacca gcaccgccga tcagtactag
     5761 gaatccgaca aatgttttga tagttccttg gatcacttct gaaaatttct tcttctgaag
     5821 aacgagacca agcagtgcaa tcaaagaaac gaggatggag ggctgactga cgattgacac
     5881 aaacaaattc aacaatttat ccattgatag ttcctccctt gaatcatttt tattcttttg
     5941 acaagatctt cgttactttt tcttttactt cttcgctgtc aatgatcgat tgcaaaggta
     6001 cgagcttttc atcaggtata gaagttagcg tatctttcaa agaatgctca atgaagaaat
     6061 aatcagccat atcgctagat acacttccaa catccgtatg acttacttca aactcgtcca
     6121 catctgctcc aagatctgca agtgcttgtt tgatgttcat ctctaccata aaacttgttc
     6181 ctaatccact ttgacatact gcaaaaattt tcatatcatt cgtctccttt tcttatgatc
     6241 gctaatattt cttcttttgt tgatgcgtgt aataaatctt gaagattttg tttattagaa
     6301 agtagctttg ttaaatttgc caaagcccga agatgtgcct cattatcgat agcagctaaa
     6361 cagatgaaca aactaatggg atgggctgga ttatcaagca gatccacagg ttgttgaagt
     6421 ttcagcaaag acatccctac ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg
     6481 atgtgatcgc caatatggat aaacgcacca tactgctcca cgcgctcgat catagctttg
     6541 atataacttt cttctatttt attttctgat aatagcggct gtgcggcaaa tgtgattgct
     6601 tcttgccagt tttttggttg atccgataat tggatcattt cttctgttaa taattcagat
     6661 agcataggac gttgatcctc cttcttttct aaaagtttgt tcattttacg atttaatact
     6721 tgatagagct tttcttcgtc tacgccttct tttaattcga tataaggcag tagcgccgac
     6781 aacaattctt gggcagaagg cattgaaaat cctggtatca gccaatctct ttgtacttga
     6841 ttgatcagct gatttttttc caagtcagtc atgattggat gcagaacata gagagacttt
     6901 ttcttatcag taagtgtgag aggtacagta gaaaaaataa tatcgtaaga agtttcgtca
     6961 acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa agatcatcgg gaataattgc
     7021 tgaagttcac tttttaagat caatgaagag ctaatgccac ttggacagac gatcacggct
     7081 cttatttttc tatttctttc ttctgcgtcc gcttttctga tctctccgcc aaatagtata
     7141 gtgaaaaaac caatctcttc ttcagggatg ctcttcccta ttcgtctttc taacggaagt
     7201 aatgcttttc ttgtcatttc gtagatggaa gcatattgat gcttaatttg atcgatcatc
     7261 acattaggta aataaaaaga ataggaaata cgaaaatagg cagggaccaa gtgataaaag
     7321 acattcatca ataattcacg aaacgaagca aattctacgg ccatcaggaa ttccatgcga
     7381 tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg caggctcctg gacatctcct
     7441 tcaacgatcg atgctaagca aagagagaaa aatataatct ccgaattttg atgttcggaa
     7501 tagccaaggc cagtcaatac ttgtcgacta atgtcagtga ctgcaagaga agccaacagt
     7561 tcagcatctt ttaccagttc catggatgtt tttccaattc gaatggttgt caaactcaaa
     7621 taataagcta attcatccat tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt
     7681 ttatctagca aatctctaac tttggcgtaa taatcaatgg aaagggttgc ggttaaatac
     7741 gtgagcccaa atcttccagc aagtgactgc atcaaacgag cagtgcgatt ttttgcttct
     7801 cttcttaata ttgcttctga ccctagcagc ctaaaaccag ttttcctagt atacgacagc
     7861 tgacaatctt ccacttctaa ttcatttctt atctttttga tatctgacag gactgtccct
     7921 ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa
     7981 accatcaaat aaagaagatc tcttcgctct tgttcggtat acacagcttc aaatcgagta
     8041 atatccaaaa atttttctgc ccagtcgatt gctcggaatt tctttatacg gatacgggta
     8101 tcatccacat attcggcaaa aggagttaat tgtttgatcg cctcgcttgt atttacttcg
     8161 atgatctctg ctgctttttc tagactgatt tctcttggtg ttttcaggag ataactaatt
     8221 ttgtagttca cagcgctgtt cacctcctac ttatattcta gtatggtagc gcttttaaat
     8281 cgaggggtgt ttggtacaac ttacagttgg atttttttta cgactgtacc gaacgatttc
     8341 tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc tcctgctttt atgaattgat
     8401 ttcgtccgat caatgatgat catgtttatt ttttgaggtc agttcttcaa atttttcttt
     8461 gacatcttca accgcttttt cgatatcttt ttcaaagtga ttgggctcat taggctttgt
     8521 ttcctcttct acagaatttg aaccagataa agctgtttct ttattttctg tttcaccaga
     8581 gtttgtgcca gctaatgatt cttctttgtc ttctgtttca ccagaattag ttcctgctaa
     8641 agccacttct tcttctgttt taggggccat tgaacctctt aatcgtttgt gtttttcacc
     8701 gtcataattt gtcatgttgg ttcctcctag tagttctata ctttttcatc gtacaataag
     8761 acagtttatg cgtcaaaaaa caaacccgat tcttacaaat atgtcatatt tttccttctg
     8821 attcttgaat gcttttgatt ccttcttcca aaattttcat ttgtaagatt gtttcttcat
     8881 ctttgatgac tttttctgcg ccattaacta atgtctcgta aatcccttcg tagacacggc
     8941 tgtaatcacc aacctcggaa attacttttt cttcatgata actcccttgt tcatctatat
     9001 aggtcaatac accataatgt tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt
     9061 aaaacatttt cagatgttct tcttgtcgat cttttgtttg tttcacgaac atcccttttt
     9121 ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa ataactggat tttatcgaaa
     9181 ttttcatatt tccatagaaa aaatccagat cgaagtaatc attcattcgt cctttcccta
     9241 aaagttgacg tacatcatag ctgatatcat ctggttcgcc gaaataagaa agaacttgat
     9301 ctactgtatg acaagcatgt ccatataaat agctgttcgc agtagaaaat tcatgcgtat
     9361 tttctggtac ttccggtctg aaataatcat aatgcatttc tacttctaaa atatccccta
     9421 attttccgct ttcgattact ttttggactg tcaagaagtc agaatcgaac cgtctgtttt
     9481 ggtaacattg gataaataga cctttttctt ttgctaatgc aaataactct tctgcttcct
     9541 ttgacgtttc tgcaaaaggt ttctctacta gtacattctt accatgttcg agtgccattt
     9601 ttccatattc ataatgaaat tgactaggtg tagtgatgac aactaattgg atctcaggat
     9661 catcccaaag atcagacagc tgatcagtat attcgatgtc ttcgaatgtt ttccacactg
     9721 gttcagatgg ggaaaaaatt tttttgatat tgattttgtt cgtaagtttt cttgaaaaag
     9781 gcaaatgata gcgatttgta ctctttccat tgccaagata agcgattgta agcatgagtg
     9841 gtgcctcctt ttattgatgt tttcagtata tcctagaaaa gcgcagaaag tatttataat
     9901 agctattctg gtaaaaaagt tcagaaaaac acttgttatc attcggtttg gtgaaattaa
     9961 tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga accaagtgtt tatcagtaag
    10021 atagaatttg gaggtcagat catgcttatt gaagaaaaac taacaaaaca agagttgttt
    10081 accacgacag agaaacggat tgtcgattac atccgtagga atatcgaagc tgcagtctac
    10141 atgacgattg aagaattagc aaaagctacg tatacttccc attcagctat cattcgttta
    10201 tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt tcgagctggg gcgggaagtt
    10261 catcaattga ttgctcagtt caatcaaacg attgatccta attttccttt ttcaagtgaa
    10321 gatagcactc agcaaattgc aaagaaaatg gcggatttat cgatccagtc aataaaaaaa
    10381 gcacaattgc agattgaaca tgaagattta gatcaaatcg gtaaagtatt gaacaaggca
    10441 cagcgaatct ttctttttgc aaaaggtgat tctcaaatca cggcaaggaa attccagaat
    10501 aaaatggtca agttgaataa gtttttgatc atggctgaag aatacagtga ttcttcttgg
    10561 aatgcagcca atttgactag cgaggattgc gccattttta ttagctacag cggtcgcatc
    10621 catcattatg aacgaataat gacctatttg aagcatgtag aggcccctac cttgctgatt
    10681 accggaaatc agcattcaga aatggccaaa caggctgata tgtgtcttgt tatatcgcag
    10741 gaggaatatg attttgctaa agtagcgact ttctcctctc aaattgcctt cgattacgta
    10801 ttaaatacgt tattctcagt gatatattct caaaattttg aagagaatat ccgcaacttg
    10861 aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa
    10921 aagatcctca tttagtaata agaaagatag tgtttgatag gctctctttg atatcatcat
    10981 aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt ttgtttacgg aacgattcga
    11041 atagatatat tatagaaatg aggaatagct aacttctgtt tcttattttg aacgattgac
    11101 gaaaaagata tataagaagg aggaaaatca gtatacatgc agctaacaat ccatacagga
    11161 aaaatcgttt gttctcggat actttctgta cttctttttg aacttctttt gcatgataag
    11221 gaacccggtg ccctctgaca agtaagcgat gactgttgac catataaggt gtacaagtta
    11281 ggagtgtcac gaaatcctgc cctttttcga ttctcagatc ttttgtatca gtcggttcta
    11341 ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc attgatttcg atatagaaag
    11401 aatcacctaa agtcagtttc ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat
    11461 ggctggaaat aacggcatgt gtattttctc cccccgtagg ataagacgtt ccttccaata
    11521 atgaggcacc tttttctagc agcacagtat tcgtttcatc aaaaattggc aaatggacat
    11581 tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc aaaataggtt ctatcctttt
    11641 tcgcaggttc ttttttctta gtaaagggat ctgcacctgg atttccacct tcttttgcta
    11701 attgctggtt tttctttgtc atgttttctt ggattttagc catgacttct tcattttctt
    11761 tgaccgcttg ttgctgataa tgagagatga tctgctgatc cagataatta ttcagcgcgt
    11821 cgctaacaaa tggataagct agtatcccta ttcctgagag aagcaagaaa agcatcagca
    11881 gatctagaag tttccttttc tttggtttca tttgtgttct ccttgtgatg actgtttgat
    11941 aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa
    12001 gactaaatct gactgtgctt gcgtctagca aagtacagtc cagcaatcag tagaaggact
    12061 actcctgcac cgatatacac atagattccc ttaccgcctg ttgaaggaag tgtacctttg
    12121 tgtttatttg gtattttttc aggagaaatc aactgtcctg ctgttacata agattgttca
    12181 tcgatagtaa atgctacacg gtttgttaat ggaacatatt tttctggcgc tttcgtttct
    12241 tccagatagt acgtgccata ttttagacct gtcacatcga ttaaaccgtt acttgtggtt
    12301 gtaaaaaccg ttgctgattc tttcgccgat acccagctga cggcttttgt ggatgggtcg
    12361 atcgataaat atttcgctgt gtcactatct tgatcacgaa cgacgaattc tgctccagca
    12421 agtgtttggt ctgatgtaac gtcaccatct actttaacaa atcgtttgcc cccagtaacg
    12481 acatcgactg acggtggtgt ttgatcattt gtatgcccgt tatcgacatt tgcttggtta
    12541 gaaaatcctt tggttggatc tgctttttcg ttcaaatgca tatagtaaac gaatttcaat
    12601 gtaccgccag gagttaatga aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct
    12661 gtttgctcag tgacagaata atttactggg tcgatttctt tatttccatc atataaggca
    12721 taagcatacg ttcctgaaga atcattatca aatgttaaag cagcgtcatg agtatcgata
    12781 agtttgaatg ttgtgtactt gttttgcgtt ccttctttat cagcgatacc taatgggata
    12841 ttgacagaaa tctcatattg aattttttca ccgattgcga catcttttcc attcaattga
    12901 ggtgttgttt tatcaacttt agacgtatca tttttgatgg tcttttctac tggtgtttgg
    12961 ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt caatcatttc cgcattatca
    13021 ggagctttta cttcttctaa attatatttt ccaacttcta aatgattaac gatcaactgg
    13081 cctttttcaa tagatgcctc ggcaaagtca ttgttgccga tgtcataaga atgaccagta
    13141 atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt acaatccatc tgtgacactt
    13201 tggatgtatt ttttgacgct tggtgttcct tcttctttag aaataataaa ttctgctcca
    13261 tttagtgctt cgttttcggc agtaccgatt tttgtaactt tcaacgttcc atcattaccg
    13321 actgtatttt tagggtagag atggatagta tctagttctt ctgtcccgta tttataagag
    13381 ccatctgctt gtttgatcat ctcatataca gggaaagcta aaaccatgtt tgcggcagct
    13441 gacactccgt cttttggttc ttctttgatc gtgtagactg catctttccc attttgtttt
    13501 ttaggtaaag ataaagtgac atttccatca gcatctgtcg ttcctgaagc aactggtgta
    13561 ccaggagtca aagactggac tgcttgtttt gctgcatcca cggacgctcc tttatctcgt
    13621 tgcgcataaa attcttgagt gacgttataa actgaaaatg aaatatcggc taatccttgg
    13681 tattgatcga attcgctcat ttctttcccg ctgttttgga ttaaaggatc gggtaaatca
    13741 gtcattttct ttttatgaag gatgacttga gctgctgtct cctctgcaaa cacttttttt
    13801 gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa ggactcctaa cataacgttt
    13861 atttttttat gatttttcat tttttcactc ctgttctttt tttgataaac aaccagattg
    13921 caaagcttgc gaataccata ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt
    13981 ttggaagaaa tggacggcta ttttcttttt tattgactac tttcaatata ctgtccgcct
    14041 ctttatcctc agaaagaaaa gttgaaatgg taaacggaat ttctgtttca cttaaaatat
    14101 aaccagaagg tgcttcgatc tcttttaatc gataacttcc tgttttcaac ccggaaattg
    14161 aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc tgattcgttc tttgtccact
    14221 tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt gtttttaata aggaatactg
    14281 cgccagctaa tcgtttatct ggtttatttg tctcgatttt gacaaattgc ttactccctg
    14341 tatatacaga gactttcttt gtactgacac caaaaccaaa atccagtgtt ccgttgttgt
    14401 tgatttcctt gttcgcctgt gctgtactgc ttagctgcat ctgataagaa atggtcagtt
    14461 tttttccagc atatttttcc aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt
    14521 tcttgtgtat gacaaagcct tctgtcagct tttctccatt cgatgaaatc gttaaactat
    14581 taggtaaaaa cgttaatgct tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa
    14641 ggatatttac aggaattgtc gtatgcaagg aataagtgat tttttcacca atcgtaaaat
    14701 catttctagg ctcttctaat accttctcaa aaggtggatc agggtagtcg ttttcttcat
    14761 ttttaggata caaatggata gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa
    14821 caactaccat attctctgcc ttttctttga cgacttctgg tactttggat tcaatgaaaa
    14881 gatacgcttt atctcttttt tcttgatctt tagcggccag agaaaaagaa gcgataccat
    14941 cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt
    15001 tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc cttttcccgt agatggtaaa
    15061 aagaatctgt gacatcataa acttggaatg tgacaccatt taatcctcga tacgtttgta
    15121 ataaagcttt ttcttgtccg tcatttggat gatttttcgg cagttgcccg tttgggaaaa
    15181 gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt ttctgttgct acctgagtaa
    15241 aagctggttt aaatagtaac aagaagagac acatactaag ccaaccaagt tttttcatat
    15301 ttagctgccc cctctttctt ttttcaaaaa aagataccaa atagaaaacg cacaaccaat
    15361 catccctact agatagattc ctaaacggcc aattcctccc gtttcaggta atggtacttt
    15421 tgcctgattc gtgatgtcta aagaaatctg gttgtttttg gctcctggtg acagaacaga
    15481 ttcatgatct tgtccatcca cttgaattga cccatcttcg tgtataacta tagtaactgg
    15541 ctcttttaga ccttggtatc cttctggtgt aaaagtttcg gtcagcgtat aagttccagg
    15601 ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca tcttttggcg attctaagtc
    15661 catttctggt ccctgcagtc tgaattttgc tccttttaat gtctgtccgt tgtcttcttt
    15721 tttgtgtacc gttagatcaa acgcttttag tcgattttga tgagtgagcg tccatacatt
    15781 atttgtcatc tcggtatttt ttcccgtgta aatgattttt ccatatttat cgatttggaa
    15841 ttcataattt cccggcgaag tatcatatcc ggcaggacca gctgtttcaa cgactcgata
    15901 tttaccaggt gtgagtgaat caaaactggc aagacctttt tcattcgttg tttggctgtc
    15961 aattggctga taagtaccat ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag
    16021 atttatctct ttgtccgtcc cttgcatcgc atacttacgt actgctacag gaacttcaac
    16081 aaaaggattt tcaatagtca actggatcgt atcatcggaa gtagtgactg tttttccatc
    16141 aatggttacc gtaccatcag aagcgatctt gatttcccaa gtagtctttt tgcttaatcc
    16201 atgcccttct ggagcttttg tttctgtcag cgtataggtc atttcttttt gcaatttgac
    16261 attttctgga agagaatagg ttccgtcgcc atgatctgtt agtaaagtat caatagaatc
    16321 tcctgttaat ttgaaaacag cgccaataag atccttttca cctgtagagg aatttttcgt
    16381 tattttcaag tttaacggtg tgaattgttt agtatttttc catgtcattg catctatttg
    16441 ttgagaatcg tatcctggta caggtaattc tttgattgtt tggtaactga atgcttgacc
    16501 ttgattattg tattgaggta gtgataatat ctcttgataa ctttctccgc tatttgcaga
    16561 taatttgtca atgtctgcac gttcccacgt atttgttgta tctttagctg gtttaatgat
    16621 tcgaatatat ccgtttttcc aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa
    16681 agtaacttga tctggacgat cagctagatt gttgtcaaat tcttcccata actttttgat
    16741 gtgaagactg actccgggag ctttagcaga tggtataccg aattcagcta attcattggt
    16801 atcaatgttt ggctggaaag ttgtcctgcc gttcatttga taccagaaat ttggatggaa
    16861 gtcctcattt tctgtttgga ttctcacttg gtaatggatt tggatttctt ggtcttttcc
    16921 taaatagatc tgattgctct tgatggtatt tccttctatg gaaatagatg gagtgaccgt
    16981 tgtaggactt gtccccacac tcttgactga aagtgtacca ggctgataaa tgaatggttc
    17041 tgcgattgga tcatttattt gtccattgct tacagttgct gagatctgta cagctttttt
    17101 ggctaggtat tcagagatat ctgttgcatg atcagctgat tcatagtaaa gatcgccttt
    17161 ttcatctgat gaaaccattt gtcgcatacg agactctact tctgcttttg agagaccagc
    17221 tgccggatcg ctttgaagtt ggatgccaag accatgtatt tcgattcctc gttctttgag
    17281 tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg attcttctgg atagattgtt
    17341 ttggtcaggt gcatcataga ttcttgagat tagagaagta tttcccggcc gatcttgcgt
    17401 attagaaaac tgagttccgt aataattgct gcttgattgt gcgtgtaccc gctgtacttt
    17461 ataggaaaat gttggtacac catccgtcag caaaacgatc acttttttat gtccatttgg
    17521 aacggatagc atgcttcctg catctcttag tgctttttgt gtaaaagtac caccatttgt
    17581 ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa tcaaatgaac ccatctgtac
    17641 tgcaccgtta ctgtagctat atccttcgct tgagtagccg acatatccca tattgatttt
    17701 gtctgtgata ccgctatctg ctaaagtatc gacaaaacga tcgacaccaa tctttacttc
    17761 accgatccga ttattgtcgt tcatacttcc tgaccagtct acgaccaata cgagatcaag
    17821 aggcgtgata tctttttgta catttccacg agcattcaaa taaacatcaa acaatccggg
    17881 tgtacttgtt tcctttgcat actttcgtat cgcatagtct gcttgattac cggttcctcc
    17941 atattcgata tacgaatggg tccgatcatt aggatctcca ttccaagaat ttataccatc
    18001 ccattgattt gttccgtttt tattgccttg atgatcaaga acatttgtat ttcctgtagg
    18061 ttgccaactg gctttaggat aagtgccctg atcatctgtt gtatattctg taggaatcgt
    18121 ttgattagat ggcacactca cagctggcaa ggataatgta ggcaatgtaa atgggctgac
    18181 agaattttcg ttttcttttg atgattcttc ttctatttcg tctggctttg gtactgacac
    18241 atctgtatct ttcttatttt ctgtctcatc aataatgtca tattctttat tatcgacgat
    18301 cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt
    18361 ttgtttagtg tttgatgaga tttccaacgt aattttcccg ctatttacct gataagcata
    18421 gccatctcct tcttttcttt ccacatcact taagtaattt tcgactagac caatttcttt
    18481 ttctatcttt tcagcactgg tgttattcgc atttagcttg atgttcagtg ctttttttcc
    18541 agactgatcc gaaatgactt cagctgaaat tgtttctaaa actgttggat catctgtagt
    18601 gtctgctatt gcagataatg gcacaaagtt tgataaactc aatattatca gcatgaaaat
    18661 aactttcagt ttcttccctg tggtcgtcat ttttcccctc ctttttcttt ttttctgttt
    18721 tgacgtgaat agtgaaaatt attaaaataa acaggcgtta cataatttta tttattttaa
    18781 tatgataatt attatacgaa gggcgctaaa gggaatcaca ccaattttca taccaattgg
    18841 tttcatttat gaggttgatt ccttcgatta atgagcgttt cattgaaaga aaagggttga
    18901 gggagaaaaa catatgatca ggttgttgga attaaactat agaaagatgg aaaaaatcat
    18961 tcgaattctg tccatttctg acaagtggat gactaaaaaa gaacttgcca aacgaattgg
    19021 tagttcagaa tctacatttg taaggtatat cgaagagatc aaaatccgct ggggaacgga
    19081 tgtaacgatt caatcttcac aaaaattggg gtatcgtctt gaacatttga atgtaccagc
    19141 ttatttaaat gttctgatgg aaatgtcgca aagttctaca aacagtcgtt tattaaatga
    19201 gattattgtc aatccgggta aaacgattga gtattattgt gaaaaaattt ccatcagccg
    19261 gtcttctttt acacgtaagt tgaaacaatg caatcaaatt ttagaaagct atttattaaa
    19321 agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa agtgagctcc agttgcgtat
    19381 ctttgttacc tttttctttt taagttacta cggaagacgc acgttgccat ttccaatcga
    19441 aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt cagatgaatt tgtttcagga
    19501 agaaagccca tatgagcaat ctttctttat tatgtatttt gttgtgcatc tcatgagaga
    19561 atcccaagga actccatttg aatggaaagc tggagactct tatgtacagt tgataaatat
    19621 cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga aaattgaagt acagcagcta
    19681 tataaaagta gtgaactatt ttgtcaatac gattttttca gaattttcgg agaaagaaaa
    19741 aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac tgttcgtttt catccgctga
    19801 ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta acgcaaattt attttttctc
    19861 ccgtattttc ccatacgata cgactcattt gacgatccgt gcaacttact ttgcacagca
    19921 gatgcaacac cgccagatac gtctatatgc tggatttcgt gcagaacttc aattttattc
    19981 cactctactg aatcagaatc tgatttatta tttatctagc tatatctttt ggatgcttat
    20041 cggtaatcct gaaattcacc attatacttc tgataaaaaa atactgatta tcagcgactt
    20101 aagtcttcga catagtcaat atattgaaga atatatcagt gatattcttg ctgtgcataa
    20161 aattcactct gagacaactg ctattactga agatcagctg tcgaaataca atcttctcga
    20221 gtatgatctg atcgttacga atcaacccat tttgaatgcc catgttcctc atatattgat
    20281 tgatgatagt gtgtcattcg caaatgaaga agaactcatc cgcttattcg agttatgaat
    20341 aatcaaaaaa cagctatgta taaaccaccc tgataaaaat ttcaggacaa atacacatag
    20401 ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga tttatagcga aagtctttta
    20461 acaactcttc ttttgtccaa ttatttgcaa tgagatccgt caaagtgttc attacgaggg
    20521 tcatggaaaa gtataggaaa ctaactgctt cttccgacga ttgaatggag aaatgattga
    20581 ttcttaataa ttccagccat ttttcgttca ttggagaatc agccgatagt tctctacgtt
    20641 tggttgtctg ctgatcattt cgctgggtta acaagtataa cctttgccaa tagatacttt
    20701 cgtgatctaa gtcgcagcac cactctaaat atttttcgat ccccaaaaag aaatgttgtt
    20761 tgttttcgct aatgaatttt aagatatctt gatggactat gtgagaactg cttttgatcg
    20821 tatacaaata agcatcttcc aaatcttgaa aatatttata aaaagcaccg cgcgacatcc
    20881 ccatcttgtc aacgatttct gagactttga cttggctgat gtgctgtgag caaaatatct
    20941 ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga taaattgaaa aaagtttctt
    21001 ttggcatcca catctctcct ttatctctga ttatacgtgg atagcccttc attgtcatga
    21061 aggattcatt ccttcttttt ctcttcttct gtcaagtacc catcttccat tcgataaacg
    21121 cgatcactcc attgaatcat acggacatca tgagtaacca taatagtcgc tttttgtttc
    21181 tcatgagctt ctttaactag taatttgacg acttcatatg catgatctgt atccaaacta
    21241 gctgttggtt cgtctgctaa gatcaagctt ggctcattat ataaagcgcg agcaattgcc
    21301 actctctgtc tttctccacc agaaaggtct ttaggataac tatttttaag tgactggata
    21361 tctaaagatt gcagcaagga atcgattttt gttgaatctt ctttttttga agtaacttgg
    21421 tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga
    21481 ataaatccta tttctttaaa gcgtaaatct gctcgttttt tttctggaag ctcagttagt
    21541 gagtgaccgt tgatcactat ttctccggaa gtcggtgttt gcaatcctcc tgaaatggta
    21601 aggaaagtac ttttacctga accagaagga ccaatgatgc tgacgaattc tccttccttt
    21661 acataaaaat caattgcttt caaagctgag acctttgtat gaccagaacc aaatgttttg
    21721 gtaacttttt tcatcgttaa tatttttttc atttttatta acctcctatt gctgtaattg
    21781 ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc tagcattgcg acaatgatca
    21841 aaatcccact atatagcaac cattgtgacc aaaaaacagc aaaaggcatc gcgcttggta
    21901 aaatcagact agtcaaataa gcaaacaaaa gagcaagcag aacgcctacc accccaacaa
    21961 taaaggactg tgctactaaa gatttagcga tgaagaaatt tttgatccct tgtgctttca
    22021 ttactccaaa aatagcagtc ttctgaagtg tcatcacgta catgaaaata ccgaccacgg
    22081 cagcaaccac taagaacaaa aagtatatca tcgcatttaa ggtcatattt tgggcagaat
    22141 agccaggaat actttcaatg aatgtcccaa tagttaataa ctgtagaccc ttcgatgctt
    22201 tttctttcga aattttagct gaatccttta ttacgatccc attgatcggc tgttcttctt
    22261 cagaagcaaa cggctgttgt ccatacttca aagcagtcca cgtatctagg gacgcataga
    22321 tgacgggaga aactgtgtaa taagtttcag gaaagatacc gacaatcgtc aaaggatcgt
    22381 cgtaactgcc aattttgatc gtatctccaa ttttatagcc tgaatcagcc agattttgag
    22441 agatgatgat ttcattcttt gttttaaatt catgcccttt cgtgagtttt ggtaataaaa
    22501 atgcttcttt cgttgtgccg aaaactgtaa cattttcttt gttttttcct ttgattgctc
    22561 cgctgtacaa tccgattggt gctttctctc ctccttcaat gtatttcaga tctcctcttg
    22621 ttaattgtga agctgcaaat acttgatttg catcctccga caaaacaatc tcttgtgcgt
    22681 cccaatcatc gattgctttt ttaaactctt ctgaaagtcc attcgctagt cctgacaaca
    22741 tgaatacgac atacgcaatc aagaacataa taccgacgat caagccatat cgtaatttag
    22801 aatagcgaat ttctttgata gctagaaaca tttgtttttc ctccctatga tagattgata
    22861 gtgacaaaat gtcacttgtt gaatagtgta actctaaagt gacaatttgt cacttgtgtt
    22921 gtttttttct tatcaataaa ttgtctaaga catctttcga taaaacctta acatgagtca
    22981 taaaaaattt atttaagcct tatttatgct ttccatgata aactaaagtg accaaatcaa
    23041 tgaggtggga aaatgattaa actattagta gtagaagacg ataaagtatt atccaataat
    23101 ataaaagaaa tactcgctgc aatcggcgag ataacacaag tttatgatgg tagtgaagga
    23161 ctgttcgagt tggaaagtgg agtttatgat cttgcaatct tggatttgat gatgccggaa
    23221 atgaatggat atgaagtgtt agcaaaaata cggaaagaaa aaatacagac tccagtattg
    23281 attttgactg caaaagatag tttggaagat aagattgaag gatttgaacg aggtgcagat
    23341 gattatctaa caaaaccttt ctatcgagaa gaactgatca tgcgcgtgaa agcattattg
    23401 aaacgctcgc taggtctctt tgatgaaaat gttctgaagt ataaggatat cagttgtgat
    23461 ttaaaaagca atgaagtctt gtataaagaa caagttttgc ccatacaagg aaaagagttt
    23521 gaattgctcg tttatttttt acaaaataag ggaatcattt tgacaaaaga gcagatattt
    23581 gaccggatct ggggatttga ttctgatacg acgattacag ttattgaagt ttatatgagc
    23641 aacttacgaa aacatctgcg aagtactgga ttggataaag aaatcaaaac cttacgtaat
    23701 gtcggttata tactgcaaag ggtgtgatgc aatgaatgga gatattacac gaaagcaacg
    23761 tagtcgcttt tttgcgacaa atgtagcagc atttgctttt atcttttttc ttttgggatt
    23821 cattacttta cagatattaa accaatctgc atacagagaa acagatctgt cattagagaa
    23881 aataaatttt gattctcgaa cagtacaatt agagatacaa agatatcagc aaggcgatcc
    23941 ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac atggaacagc cattaatgga
    24001 accatcaaat gaccgtttta acactcaagt gatcttgtgg tctaaaaatg gtgagatatt
    24061 gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa
    24121 aaatctgaat accatccaaa atattgaatt aaatgaagac agtggctcat ctgccctctc
    24181 tttccgttcg attacgaaaa aagcggagac gaatcaagat ggcgttgcct acatccaaat
    24241 tttagaaaat acaaatcaaa tcaagaattc attagcgact tttcgcacga ttatgatttt
    24301 gtgtatgatt gttttttgga ttctctctat tggtatcagt tattacttgt ctagtctaag
    24361 catgcgccct attattcttt cttggagaag acaaaaagaa tttgttgaga atgcttctca
    24421 tgaattgcgc acaccgttaa cgattattca aaatagtttg gaacatcttt ttaccaagcc
    24481 taatcatact attattgaag aatccgaaag catcgctcaa gcattgtcag agacgaggcg
    24541 cctcactgga ttgacttcgg atttgctgac aatcgctcgg aatgattctg atcagcagct
    24601 gctttcaaag caaatgatca atacacaaga atatatcgaa caacttgtta aaccatttca
    24661 agagatggcc atcatagatg gaaaagagtt tattctccat aacgatgcaa cattagaagt
    24721 accaattgat gaaaaaaaaa tccatcaagt tttggttatt ttgctggata atgcattaaa
    24781 atatacgaat ccagaagata agatcatttt ggattcatct gttaaccata aagaatggct
    24841 gattcacgta aagaatactg gcccttctat cagtgaagaa gatcaagcaa ggatttttga
    24901 tcgattttat cgggaagatc aatctcgggc gaaagagact ggcggatacg ggcttggctt
    24961 ggcaattgca aaacaggtaa tcgaacagca tcatggacag atttctgtat ccaattatct
    25021 tccacaagga gtcgatttta aagtccaatt gccgttgaag cctaaaaaat aatgcttatc
    25081 cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt ttttgttgcg aataaacggt
    25141 gttccagaag tagcttcttc ggaaataagc cgaaatttct ttaagctgac cacttctgtc
    25201 acaacctctt tagcttacgc ttacgatttt ttattgcggt cttcaatgtg tacatcaccg
    25261 cttgtgtcaa tatccaattc ttctcggcta acatcttctg tcactttttc aacatcttca
    25321 tgttcttctt tatgaatccc aacttcttca cgaataactg tatgtttgct tacatctact
    25381 tgctcttctt tgattgggat agtaatcgtg tcatcttcca atttttcgtc tgttccctgt
    25441 gaagattgat ctgttactgg tttgcgttcg atgacgattt cttcacgttt gaccggtact
    25501 tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt cgacttctcc agttgtcaca
    25561 tcatgtgtat ttacatctaa tttttcttct tttagttgaa ttgtatcatc atctagattt
    25621 ggtttgcgat cgatgtcttc agtcttgctt gtacccattg tatcagtctc gccaggtacg
    25681 tccatcggat cagttcctgg tgtcatattc aatggatccg ttgtattttg aaagcctgtc
    25741 gtgccaccta cgtccattgg atctgttcca gtgccagttt ctaatccagt ccccattgtg
    25801 cttgctgggc taacagtatc ttgtgtatcc attgtatctt ctgttgtttc tggctgacga
    25861 tagcctttta ctgtgatgac aaattttcca tcagaaatat ctcctctata ttgatataaa
    25921 ggatcatttt ctggtgtagc cgtctcagaa tcataggtat caaaagaaaa agtatctttt
    25981 attttttccc ataaagaacg gtcttcttca ccatccatcc gattttcatc tgtttcaaca
    26041 tctataccag acaatccgtc aaatcctagt ctagatgctg cttcatcagt agtataaata
    26101 acgatatcat ctcgttcata cccttcgtta cgcaaccgtt caattactga taatgcttct
    26161 tccctcgtac tatagcttcc gatgaccgtt aaatttttct cttccatgta aattcctcct
    26221 agcaaataga tttatgattg gtatattgtt a
//
LOCUS       DAYYAR010000023        26237 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000023 DAYYAR010000000
VERSION     DAYYAR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.023"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..268
                     /locus_tag="ACHAT0_000879"
     CDS             2..268
                     /locus_tag="ACHAT0_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450841.1"
                     /translation="MEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLASKKTNSFGLI
                     LSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(912..1865)
                     /locus_tag="ACHAT0_000880"
     CDS             complement(912..1865)
                     /locus_tag="ACHAT0_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8450842.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1888..2643)
                     /locus_tag="ACHAT0_000881"
     CDS             complement(1888..2643)
                     /locus_tag="ACHAT0_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450843.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2640..3599)
                     /locus_tag="ACHAT0_000882"
     CDS             complement(2640..3599)
                     /locus_tag="ACHAT0_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8450844.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3592..4542)
                     /locus_tag="ACHAT0_000883"
     CDS             complement(3592..4542)
                     /locus_tag="ACHAT0_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8450845.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4692..5144
                     /locus_tag="ACHAT0_000884"
     CDS             4692..5144
                     /locus_tag="ACHAT0_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8450846.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5233..7500)
                     /gene="gshAB"
                     /locus_tag="ACHAT0_000885"
     CDS             complement(5233..7500)
                     /gene="gshAB"
                     /locus_tag="ACHAT0_000885"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8450847.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7687..8685)
                     /gene="ftsY"
                     /locus_tag="ACHAT0_000886"
     CDS             complement(7687..8685)
                     /gene="ftsY"
                     /locus_tag="ACHAT0_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8450848.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8696..9505)
                     /locus_tag="ACHAT0_000887"
     CDS             complement(8696..9505)
                     /locus_tag="ACHAT0_000887"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8450849.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9514..13095)
                     /gene="smc"
                     /locus_tag="ACHAT0_000888"
     CDS             complement(9514..13095)
                     /gene="smc"
                     /locus_tag="ACHAT0_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8450850.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13115..13801)
                     /gene="rnc"
                     /locus_tag="ACHAT0_000889"
     CDS             complement(13115..13801)
                     /gene="rnc"
                     /locus_tag="ACHAT0_000889"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8450851.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14149..15930)
                     /locus_tag="ACHAT0_000890"
     CDS             complement(14149..15930)
                     /locus_tag="ACHAT0_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8450852.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15956..16870)
                     /locus_tag="ACHAT0_000891"
     CDS             complement(15956..16870)
                     /locus_tag="ACHAT0_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8450853.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16897..17859)
                     /gene="opp4B"
                     /locus_tag="ACHAT0_000892"
     CDS             complement(16897..17859)
                     /gene="opp4B"
                     /locus_tag="ACHAT0_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8450854.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17859..18803)
                     /locus_tag="ACHAT0_000893"
     CDS             complement(17859..18803)
                     /locus_tag="ACHAT0_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450855.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18804..19808)
                     /locus_tag="ACHAT0_000894"
     CDS             complement(18804..19808)
                     /locus_tag="ACHAT0_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450856.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(20076..20318)
                     /gene="acpP"
                     /locus_tag="ACHAT0_000895"
     CDS             complement(20076..20318)
                     /gene="acpP"
                     /locus_tag="ACHAT0_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8450857.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(20440..21441)
                     /gene="plsX"
                     /locus_tag="ACHAT0_000896"
     CDS             complement(20440..21441)
                     /gene="plsX"
                     /locus_tag="ACHAT0_000896"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8450858.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(21490..23526)
                     /gene="recG"
                     /locus_tag="ACHAT0_000897"
     CDS             complement(21490..23526)
                     /gene="recG"
                     /locus_tag="ACHAT0_000897"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8450859.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23643..25325)
                     /locus_tag="ACHAT0_000898"
     CDS             complement(23643..25325)
                     /locus_tag="ACHAT0_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8450860.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(25377..25739)
                     /locus_tag="ACHAT0_000899"
     CDS             complement(25377..25739)
                     /locus_tag="ACHAT0_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8450861.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            26009..26197
                     /gene="rpmB"
                     /locus_tag="ACHAT0_000900"
     CDS             26009..26197
                     /gene="rpmB"
                     /locus_tag="ACHAT0_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8450862.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7214 a         5710 c         4640 g         8673 t
ORIGIN      
        1 tatcgaagaa acacaaaata tcgagggttc ttatattgaa gtaaaaacac tctttgtcaa
       61 tgaagataaa accaaagcat tggatattta taaaaaatta gcttcaaaaa aaacgaatag
      121 ttttggattg atattaagcg agtataaaat caaagcagaa gagagttatt tctatcaatt
      181 actaaaacgg tggagtaagc ttcccgccga tttttatcga aaaatgcaga tcatcaacta
      241 tcaaccgctt gctgagacac atgcctaaat agaggttgtg aaatcagtgc tttgagctaa
      301 taccgaaggt cagctgattg aacattgttt agaagagatt gtgacaactg cgatttgctt
      361 tgagtaataa gaacgctgga cagaaaatag cttctcatat tttctagtct agtgggctta
      421 ttgccgagaa gcagcagatg gaacatcgtt tagaagagat tgtgacaact gcgatttgtt
      481 ttgagtaata agaacgctgg acagaaaata gcttctcata ttttctagtc tagtggactt
      541 attgccgaga agcagcagat ggaacatcgt ttaaagaaga gatcggtaca aaataaaaat
      601 atctaaattt aagttttatc taagtttcgc tttgtacctt tgattcaaat caaaggaggc
      661 gatcttgatg ctgaaaaaat ggatcacact attttttgcc actccccatt ctagaatcaa
      721 cccgtatgat gtagataatg aattcacgtt gaaaaaagat ttctcaaata aataaaaacc
      781 atccgtaaaa tcatcaatac gattttacgg atggtttttg ataacttctt tgaggatagg
      841 ctcttgctat ttaagaatgt aagaaacatt attgttacat agtatgagag tttaatcctc
      901 tgttatttat gctatttaaa tgcttgattc acatcttcga tcatcagttt cattgattct
      961 aaaccgcccc cgcttaaata ccagacatct ggttcaaggg agatgatctg ctgattttta
     1021 ccagcattcg tttgtgctac cagttcattg gcagaaatat catctttgct gtcgtctcca
     1081 ccaatagctt ttgttctgtc tactacaaat aagatatccg gatttttttc cagcacatat
     1141 tcataagaaa cactttgacc gtgagtggat gcttcgattt gatcatctgc ttgttcaaag
     1201 ccaaataaat catggatgaa accgaatcta gaaccagatc cgtaggctga taattgccct
     1261 tcattcacaa gtgtgactag tgcttttttg tcagtagctt ctgctttctt tttcgtctga
     1321 tcgattgcac tagataattc ctctaattga gttttagctt cttcttgctt accaaagatc
     1381 tcccctaaag cagtgacatt ttgctggaaa gattcccatg gatttttcgc atctaaagaa
     1441 aggaaaatgg tcggagcaat ttgacttaga tctttttgaa aatctctttg tcggccagaa
     1501 atgatgatta gatctggctg aagctgatta attttttcta agtctggttc ttttattcca
     1561 cctgctgatt ctactttttt ataggatgaa agatactcag gaaggctgct tgtagctgct
     1621 ccaactacct tatctccaac accaagagca tccatcgtat ctaaagaacc attgtcgaat
     1681 acgacgactt tttctggatt atttggcacc tcgatttggt ctccattact atctgttatc
     1741 gtcaatgtag tttcagtttg tgtgctgctt ttttttgtgt ctgcctgact ggtgttgttg
     1801 ctatttccac atgctgctaa accaaatgat gctagaatca agatacctac tactaatttt
     1861 ttcatctctt ctctcctaat gttagtttta gaaataaaga cagaatctgc ggccttcaat
     1921 ctcacaaatc cgtatgtcca tttcgtataa ttcatccaat acatcttttt ttatcatttc
     1981 ttgggtcgtt ccttgcgtaa ataactgtcc atctttcatt gcaacaattt cgtctgcata
     2041 acttgcagca aagttgatat catgaagaac aataacaatt gttttcccga tttcttgtac
     2101 taaatttttt aaaatctgca tgatttgaac cgcaaaattc atatctagat tgttcaaagg
     2161 ttcatccagt aaaatataat ccgtgtcttg cgctagcacc attgcaatat acgcacgttg
     2221 gagttgaccg cctgaaagag tgtcaatcga ttgatccgct atttcttcta acccaaggtg
     2281 ttccagtgat tcctgaacga tttcctcgtc ttttgtcgtc aattttcctt tcgtataagg
     2341 aaatcgtcca aattctacta attctttcac ggtgatttgg acatttattt cattcgattg
     2401 tcgtaggatc gataattttt tggctaattc attggatttc caagccttta cttcgttatg
     2461 gtctaaatag atttcacctg tatcttttgg tatcaaacga ctgatcatcg acaacaccgt
     2521 tgactttcca gcaccatttg gaccgataaa agcagtcaac ttcttttcag gaatcgttaa
     2581 ctgtacatct ttaaccactg attttccatg gtacagttta ttgacctttt gtaaatcaat
     2641 cataggatac tcttcctttc gcgaatcaat tttgtgacaa aataaattcc tccgacaaat
     2701 tcaacgacga cactcactgt tgtattccag ccgaaaatct gctctactgc gagctgtcct
     2761 cctgtcagaa acagcatagc taccagaaag cttccgataa atagctgacc atgacgataa
     2821 gtagaaaaga cacgataact gatatttgcg acaataaacc ctaaaaaagt aactggaccc
     2881 acaagagccg ttgatgtccc tgtcaaaaga ctgacacaac ataaaatcaa aaattcaaaa
     2941 cgatctgtgt ctatcccgag gtttactgct tgttcatttc cgagatggaa aacgtctagc
     3001 acatgtcgat atcgaaataa caggtaccag cagccgataa tgatcaaaga agcaggcaaa
     3061 agatactgac tctggatatt gccaaagctt gcaaataatc tgccttgaag caaatcatat
     3121 tcattcggat ccatgatgac ttgtaaaaag gtactgacac taccaaaaag cgtacctaaa
     3181 atcatccctg acatcagtaa taaaaaaaga ttcgctctca tttttttcaa aagaaagaaa
     3241 aataacaata gactcagccc gaccatcagc ccaacgttca ataagaaaaa aactgtacct
     3301 gtctgctgaa gcatcgtcac tcctccgata aagaagaaaa ggaccgtttg gacaaggaca
     3361 tataacgact caagtcccaa tactgaagga gtcaaaaact ggttatgcgt aattgtctga
     3421 aagctgattg tggcactgct gacagctagc gagacaagga taaatgcaaa aattttctta
     3481 ctcctcaatg cacatgcaaa atgccagttt ccatatgtat ggatcgtcaa gtacattcct
     3541 gtaacaaata aaacaagaac aagcatacca ataaaaatag atggcttttt tttaagcata
     3601 tcgtcgtccc cttatcagaa gataaaggaa gatagcccca ccgatgattc caacgatcac
     3661 actgactggt atttcataag gaaaaatcaa cacgcgagcg acaagatcgc aaaatactaa
     3721 gaacaaacta cctgctaaag cagttacgcc aattgttttg tgcacttgat ctccatatat
     3781 tctagagaca agattcggaa tgatcactcc gataaaagga atgcttccta ccatgatcaa
     3841 catgactgca ctagccaatg atactaagcc aatgcctaaa aactgcatct tctggtaatc
     3901 tattcccaga tttactgcaa acgattcgcc cagaccaatg atcgtaaatc ggtaagcaaa
     3961 tagccaaacc aagagcaaca atggaacaac agcatacaat aactcataat ttcctcgtat
     4021 cacagtcgca aaattccctt gaagccatga agtcatattt tgtaccaact ggaattgata
     4081 agcaaaaaaa gtagcaactg aaccaatgat attccctaac atgactccaa tcaacggaag
     4141 catcaattga tttttctgga ggaacaatcg cgtaagggat aaaaaaagca atgttcctgc
     4201 aaaagcaaag caaaatgctg tcaaggatcg gacaatcaag gatgcaccag gtaggaaaat
     4261 catagccact aaaataccta aacgcgcact atccatcgtg cccgcagtcg ttggtgaaac
     4321 aaatttattt tgggtcaaat gttgcatgat caacccacaa atactactgg tcgctcccac
     4381 aaggatcaaa caaatcgttc gtggcaaacg agtcactagc aagatttgta agctttgccc
     4441 atctaactgg agcagttctg acaattgtac attcgatact ccgatgaaca atgaaatact
     4501 acttaaaata actaataatg ctactagtcg tccgtagcgc atccaatcca cctttctaat
     4561 tgagaatgat tttcattctt attttaagtg aatcgttgaa caaaagcaac cgctttttca
     4621 tatgttttag gtattattgg gaatatgcta taatcgtaag aaagacaaag agaagaagga
     4681 gggtattttt tatggcacaa gatgatgtaa aaagccattt ggataaggga ctttatggta
     4741 cacctttagt gaaccctgaa gaacagcata aatatttagg gacattcagg gaacgctgct
     4801 atcttagtat gactgttgct gaaatgatcc aaccaaaaaa caaagaaaac tttttaaaag
     4861 aactcaaaaa acatccagat gcttccatac tcttgaatgg ggctctaccc ctttctgtcc
     4921 aaaaccaata tatccagcta gcaacgaaaa acaatagccg tttcactgta gtcaacgatt
     4981 acgtcactgc aaatcctgag gaattcggat tgctcttaac agcaaaagaa gcagtaaatg
     5041 aacctgtgat tgatatcgaa aaaaaatacc cagaagaaaa ctcttcgcca gctgaaaaag
     5101 aaaaaccaaa aaagaaaaaa agcttctggg atcgtttatt ttaaaacgaa aaaacacata
     5161 aatttttaca tgcaaaaaaa gcttttgatc agattgacat caaataggac aatctgtcaa
     5221 aagctttttc ttttaaaata cttcaggata taacaatttc aatacattca tcgtcaatct
     5281 tctgcctttt cctgcaaaag gataaacatg catatgcatg gctggattga aatttgcttc
     5341 gatgatccca tatgcttttt tatcagttgt aggatcgatt tctttatctg gtataatcaa
     5401 atcgatacca ctaatcttag ctcctagtgc ttctaccgca gaaacggcga tttttttgta
     5461 agcttcagaa aattcatcag tcatatcgat cgaatcacca cctgtactga tatttgagtt
     5521 ctcccttaga taaacaattt gattggcttg gggaactgat tcgatcgtca acccttgttc
     5581 tttcaacatc aattgttcta attcacctaa ttggatcaat tctaaaggtg cacgatgatt
     5641 cgttccacgc aaagggtcac tatttttttc ttcaacaagt tctttcactg ttcggatact
     5701 gtcaccaata acattagcag ggactcgcaa catgatggct tggacttctc cgtcgatcac
     5761 aaagaaacga tattccgttc ccggcataaa ttcttctact aaaaccgaac tgtcttcacg
     5821 aaaagcaatg gctaaacctg cttgataatc ttccagacta gctccttcct tgaaaatcgt
     5881 gatacctaac ccgtaattgg ttgatttagg tttgatcaca aatgcctgct cggcaaatct
     5941 cggataagct tttaccgctt cttccatgga tgaaaattct gcgcctcctg gtacccggaa
     6001 cccagcttct tttaagactt ttttcgttac tgttttgttt tccatgataa gtgggacgat
     6061 atagctgtcc ttacttgtca tgtttgcatt tttcacatat tctacatggt cttggaattg
     6121 cagacgcaaa aattgatccg actcatctaa tactttgact tggacacctt tttggatggc
     6181 atcgaacata aatatctgag tagaaagttc catttcgcga aaaccggcca actgataggg
     6241 acgttcatgg ctttctttat aatattgatg tcccaatgcc acagcaactt cttgctgact
     6301 cgatagctgt gcttcttgat agagtttcgc tgctaacgtt tgttcaggat gatccatttg
     6361 agtcaatgct tcctctacta aataaagaga atccaaccaa tccaattcaa ccagcatttg
     6421 cttcattcct tcaagaattt ctctaccttc ttgatgaaat tctgtttgat ctgatggttg
     6481 ttctagtgct actttgttat ttcgagtcgt accttctgct acccattggt cacagtcttc
     6541 tttttcttct gtccataaca tgtacatcag gaataattgt aagaaccgaa cttgctctgg
     6601 attgataccc aatcttgcat aaggattcaa atcgatattc cgcaattcaa tataacgaac
     6661 acctgttgtt gctaaatctg cgactttctt tccgcctcgg aaacgaagcg gtgtataaaa
     6721 ttctttctcc tccgaaagtt tcccttcttc gatcattcgc tcgatatctg ctaaatactg
     6781 tttcatagaa gcataggaaa ctttcacatt tggatgattc gtatagccca gcgcactgtt
     6841 gcgaatactg cgaacaggtt cttgcggatg tgattcatct aaaaagtagt tcttctcact
     6901 cattggtgaa gcaccgaata aataagtgat catccagcga tagcgcataa agtttctcgc
     6961 tgttttcaaa taaagttctg ttttgaattc ggaaaaatct tgaaattctt cctgatggct
     7021 gaacaatgtt cttagcaact catcgccaaa ttcaaaattg aaatgaatac cgcttaccat
     7081 ctgttttcgc ttcccgtatt tttttgctaa ataacgtcga tacaagacat cttcgaaatt
     7141 ttttaatttt gcaataatga tctcttcgtc tttttccggt aaggcaggtg gcatacttaa
     7201 tggccagatc atctcttctt tcggtataga acgagcagtc acatcataaa cagcagcgag
     7261 atactgaaat aattcgggaa tagaatcggt aacaggggtg atcatctctg tttgtgtttc
     7321 gctaaaatct gtttggatat aggggtgata ggatcgatcg ccaaagattg ctggatgatc
     7381 ggtttttgct agatttcctg caagatcaac ccgctgtcct tcccgttcta taccaaatcg
     7441 agcttggtcg ataaaagggc gcgcattgac atgcaataat aattgcttaa aattcatcat
     7501 taagtgacct ccaattcata tgaatctaat tataatcaaa aagtctaaga aaaagaacga
     7561 aattgtttga gaaccgctta caaataagta taaaaatgaa ggcatcaaaa acacagctat
     7621 gttaaaaaca atcattcaaa caaggagctg caacttttgt cacagctcct gctttttaga
     7681 catgggttat tcttctttta gtaatccttt gaacaatcca acgacaaaat catttggatc
     7741 aaacggctcc aagtcatcga ttccttcacc aagaccgacc aatttcactg gtaaatgcaa
     7801 ttcattacgg atagcaagta cgataccgcc ctttgctgta ccatctaatt ttgtcaaaac
     7861 aagaccggtc acatctgttg tttctttgaa ttgttttgct tgaaccatcg cgttttgacc
     7921 agtggttgca tctaatacaa gaagtgtttc ttggggtgct tctggtaatt cccgctggat
     7981 tactcgtttt attttttcta attcgttcat caagttcact ttattttgca gtcggcctgc
     8041 agtatcaact agtaaaacat ctgcctgctc tgtctttgct ctttccaaag catcgaatac
     8101 gacagctgct ggatcgcctc ctgcattgcc acgaacaact tcaacaccag cgcgttcgcc
     8161 ccagacaact aactggtcga ttgctccagc gcggaaggta tctgctgctg ccatcaatac
     8221 ttttttacct tcgtttttga actgatgggc aagtttacca atacttgttg ttttccctac
     8281 accattaaca ccgacaaaca aaataactgt tagtccgtct tcttggatat ttagttcatt
     8341 tacttcattg attccttccg cttcatagag atccacaagt ttctcgatga tcgcattttg
     8401 gacttctgcc gattttttcg catttctgag tttcacttct tgacgtaatg cttcggtgat
     8461 tcgtatcgct gtttcaaaac ctacatctgc accgataagt gtttcttcta attcttcaaa
     8521 aaagtcttca tcgactgttc gaaaattggc aaacaactca ttcatccgtt caccaaatgt
     8581 tttacgcgtc ttttctaaac ccttgtcgta cttctcctgt acttcaactt cttctgtttt
     8641 ctcttcgcca gtaaaagcac gcttgatttt atcaaaaaat cccatttgac tccccctact
     8701 ctaatacaaa tttttggatc actttggcta cgccattttc ttcattgctg gctgtcacga
     8761 aatctgctgc ttctttgacg aaaggcacag cattctccat cgctacgcct agtccagcat
     8821 attggatcat cggtaaatca ttttcctcat caccgatcgc catgatttct tcttgtttca
     8881 aaccaagatc ttttgccaat aaagaaatac cataagcttt tgtaacacct tttggcataa
     8941 attccaaaag catacttctt gttttgatga tctcatacat ttcatggaat tctgctggta
     9001 tttctttgat tttaggatct aattcttctt ggtcataagc gattactgcc ttattaagca
     9061 caaattcatc tgtaatatcg cttatatccg ctggttggaa ttgcagcagc ttattcaaca
     9121 cattgtacaa ggattgcttc cttggggcac taggcagctg taaaaccaca ttgtcagaca
     9181 aaacatctag tggcagatcc aattgctgag ccagtttgta taagcgttgg atatctgata
     9241 cttccatcac tttcttttcc atgatttcac cagtatcatt tttttgtacc aaaccgccgt
     9301 tgaaagtgat gctgtagtca ccttcatcta ccagtcctaa ttcttgcaga taatgcgcca
     9361 tcgcagctaa ggggcgtcct gtacatagaa cgatcttgac cccttgctgt tttgcttgtg
     9421 ctaatgcttg tttattctca tctgaaatca gcttttgttc atttaataat gtaccgtcca
     9481 aatcgattgc tactaatttg atcattttcg ttcctacact ttcgtttcgt tgattttcgc
     9541 ctcttcattg atatcttcca tccgtacaga aacgattttt gatacaccgg attcctgcat
     9601 cgtcactccg tacagtacat ctgctgcgac catcgttcct ttgcggtgtg tgactacgat
     9661 aaattgtgtg tcatcttgga aatcgctcaa gtaacgacca aaacgtttca cgttggcttc
     9721 atctaacgct gcttctacct catctaagat acaaaacgga acaggacaga cacggataat
     9781 ggaaaagagc aacgcgatgg ctgtaagtgc tcgttctcct ccagaaagca aacttagact
     9841 ttgcagtttt ttgccaggag gttgtacttc aatctcaata cctgttttca gcaaatcagt
     9901 tggttccgtc agtactaatt cagctttacc gccgccaaac atatttggaa agacaatttt
     9961 gaattcttga cggatagctt caaatacttc tttgaatctt gtccgcactt catcatccat
    10021 ctcatccatc gtttcaaaca attggttttt cgctgctaac agatcatctc gctgtgttgc
    10081 taagaatgta tgtctttcac tgacttgttc gtattgttcg attgaattta agtttactgg
    10141 tcccagtttt tcaatctgtt cttttaagga tgaaaccttt gttctactgt cttcgatatc
    10201 ggttgtttct tgatagtctt gggaagcttt ctcaaatgtc agctgatatt ctgactgcag
    10261 ataggtcaaa tgattatcta aaatcatttc agctcggtct ttttggactt ctaatttgga
    10321 ttgttccgac agtcgtgctt tttgctcacg attacgctct gccaagactg cttcaagctt
    10381 atcgatctcc ttttgccttc tatctcgctg ctctttggct tttgccaact cggcttttaa
    10441 tgtttctcgc tgagcagata attcgttaat ttgtttttcc aaactttctt ctgtgacttc
    10501 atgatcagaa aagtctgctg tcaatgtcgc caactgcttt tctatcgctt cttgacgttc
    10561 ggttgcctct gctttttgga ctcgggcacc gcggagctgg atctgcagat gattaaactg
    10621 ctctttcaag acagcaagat ctgcttgttc ttgtgctttt tgactttgta cttgtgctct
    10681 tctggcttcc atctggtcgc tttcttgact taacgatttg atttcttcgt cgattttttg
    10741 tcgttgcgat tcaagatctg tttgtttgtc tttcaattcg ttcgtttgct tttcgtaatc
    10801 ttcgataaac tgctgcagtt cacgtgtctc aaaagttgag atttgctttt ctttttcaaa
    10861 acgttcaagt tcatttatga tgttttgtag ctgatttgtc gcttcttgtt cttcaaaacg
    10921 taattgttcg cctcgtgtac gcaaaacttc ttgttcttcg gctaaacgag ctgtttcttg
    10981 ttgcagctcc tgaacttttt tttcttgtgc ttggagttgt ttatctgctt cttcaaattc
    11041 actagtcaac tgtttcaatt cttgattttg tacaaacaaa ctgccttggt ttcctcgttt
    11101 attcgctcca cctgtcatgg atcctccggc attcatcaca tcaccttcta aggaaaccac
    11161 tcgatattga tagcggatcg tttgcgcgat tgcattggca ctagttaagt ctttggctaa
    11221 aaggatcgtt cctagcaggt tttgaaccac agtttggatt tgatcgggat aactgacttg
    11281 ttcactggca atccctataa acccttctac agctgctgct tgtgtcaata tatgagccgg
    11341 taattgtctt ggtttgattg tcgtcagtgg aaggaatgtt gctcttcctc ctctttgctg
    11401 tttcaaatac gtgatggctt gccgagcatc tttttcattt tcaacgatga catgctgggc
    11461 tgccccacct aaagctgctt cgatagctaa cgtgaaatct gcaggaacat cgatcagttc
    11521 agccactgct ccgacaatac ccgaaagctg ctgtttatgc tgcaagacca atcgaacccc
    11581 ttggtaaaaa ccaaaatagt tttcttggat ttcttgaagg cttttcttac gagcacgtag
    11641 ctgctggact tgattcatta attgatacat tttaggctgt tcttttgcta atgcttcttg
    11701 gaactttttc ccgttttgtt ggatcatttc taacttctct tttgtttcag ttaaagatga
    11761 ttttagctgc gcctgctttt gtgtcaattc ttctttttgt aagatcaatc ggtctactga
    11821 tgcttctact tccgattgct tggctaacgt ctgcttgctt tttgctgttt cttgtatata
    11881 ttgacgttct agatacttca gttcgtttcc tacagctgct tgttcttgca tcaaatcaac
    11941 atattgatct cttaattctg ccaataattc tttcgttgat ttttggtatt tttctaattc
    12001 ttgctgtgtt tttatgagat ttgcttcagc tttttggatc tctgtttctt tttctgctgc
    12061 tgctttcatc aagctttctt gaagcttttc aaaatgttta accttttttt gtgcttctgc
    12121 taatgaagtt tgatattctt gcgagctttt ttgtgtatgc tttgtccgct cctgcaaaac
    12181 atctttttgt ccttctgtct gcttcagttt ttccgaaaga tctaataata cttgctgatt
    12241 tttttcgatc agacgatcgg cttgcgcatt ttctttacgc tgcttcgcca aaatggattc
    12301 ttgttcttgg atcgactcag atagtttgcc taattctaga ttgaatttcg caagctgtgc
    12361 ttgtttgttg tcccaatctt tttttgctgt cttgatttca gcaaccatca aagagacatc
    12421 ggtttgagtc agtgtttctt ttagccgtaa aaactccttt gctgcctcac tctgggcggc
    12481 caaaggagtt aactgttctt ccaattcgtg gatgatgtct tggacgcggc tgaggttgtc
    12541 ttctgtttca aaaagcttct gttctgcttt ctttttccgc tgcttgtatt tcaagacacc
    12601 agctgcttct tcaaagatcc cacgacggtc ttccggttta ctactgaaaa tcgcttctac
    12661 ttttccttga gaaatgattg agaacgattc ttttcctaat ccagaatcta agaacaattc
    12721 ttggatatct tttaatctgc agctttgttt attgatgaaa aactcgcttt ccccagtccg
    12781 tcgataacgg cgggttacac tgatctcatt gtactccaat ggcagataat gatcactatt
    12841 gtctaaaatc actgtgactt ctgcaatatt cagctgctta cgggtatcgg aaccggcaaa
    12901 gatgatatcc ggcatttttc cgcctcgcag acttttagca gattgttctc ctaataccca
    12961 gcggatagcc tctgtaatat tgctttttcc gctgccattt ggtccaacca ctgctgtgac
    13021 gctattttca aaatcaatga ctgtcttgtc tgcaaatgat ttaaaaccgg ctatttcaat
    13081 tcgtttcaaa tacaaagctt tccctcactt acttttacag tgctgccaat gcattttccg
    13141 ccgcagcttg ttctgctaat tttttcgatt tcccttgtcc agtgccaatc aattgatcat
    13201 ctacataaac ttcgatccaa aatactcgtt catgagctgg accttcttca ttgatcaatc
    13261 ggtattcgat tgatacatca cctgattttt gcaagacttc ttgtagtttt gttttatgat
    13321 ccatctcatg tgaaaaagca cctgctttga ctttaggaaa aatcactttt tcgatgaatg
    13381 catgggtcgt gtcgaatccc tggtcaagat ataacgcgcc taaaaaagct tcgaataggt
    13441 cgcaaagcaa tgctggtcgc gtacgtcctc ctgaattctc ttctcccttg cccaacaaaa
    13501 catattgatc aaagtgacat tctttggcaa atttcgacaa gctgtcttcg cgaacgatcg
    13561 cagctcgtgt ttttgtcaat tttccttctg gcaaatgagg atataaacgg aataaatact
    13621 gagaaaccat cagttctaaa acggcatctc ctaaaaattc aagtcgctca ttatccgata
    13681 attgcagatt gcgatgctca ttcacatagg atgaatgggt aaaagcttgt tcaagcaaat
    13741 ttagatcgtg aaaaacgatg ttatagtttt cttttagttc ttttgtcagc tggttgtcca
    13801 ttttccacat tccttttatg gatatttttt cgatacttcc ttattatact ggttttttgt
    13861 caaatttgaa atggttcggt caaaaggtaa gtaaagcttt aataatccta gtgattgacg
    13921 agaagttgtg aaaaattcgt ttagtttcga gtatgaagaa aaaattttgt gaaaatggct
    13981 ttccatattt ttgcataaat ttgacttaat accgagaaac tggactttga acaccgttta
    14041 ttggagaagt catgaaatca gcgccttgac ctgaatatta gagaaataaa cagaaaaata
    14101 tcaaaaaacc gaaaaaggac atcgcccttt ttcggtcatt tttagtgatt atgaacgtga
    14161 atctgctgta acagaaacat ttgcccatgg gtttggtaca tcataagacc aatcccagtc
    14221 tttgacacgg tcgttgactg gcaatacttc attacggaac aacgttggaa tcgcaaatgc
    14281 ttcttcaaat gcatattgtt gccatttttg atatgcttct ttttggtact cttcatcaaa
    14341 tgctttgctt gaactgatat cgttgatcaa ttttgtattt tcttctgatt cataacgagt
    14401 gtagttgaac gcagcatttg gtccccatag tccacttgga tttggatcag ttcctgtatt
    14461 ccaagcagct tggtaaacat caatttcagg atcatcgttt tctaatttat catagaaaga
    14521 attgaattcg atcagacgac ctgttgtgta ttggacatcc aaaccaattt ctttccattg
    14581 ttggatatag taatctgcca atggttgcgc tgtttcccca ccggacatcg aagcaaagtt
    14641 gattgttagt ttatttccat ctttgtcttc tcttaaacca tcgtcatcca catctttgta
    14701 gccagcatca tctaatagtt ttttcgcttt gtctagatct tgtgtgaatc ctttcatctc
    14761 atcatcatgg tatgactgga atactggagg aatcaatgaa gtcgcattgc ttctcaaacc
    14821 tgcatagaaa cgttcaccta ctgcattatt atctaatgca tagcccattg cttgacgtaa
    14881 tgatttatca gccatttttg aatctggatt gtattccaca ctctttgtat ctgcattcca
    14941 tttacctaac ttgaatccta gataagtata agaagtttgt tcacggccta agtttgtata
    15001 gccagcaatt tctttatagg tatcgtaagt atctgttggc atttggaaaa tcatatcata
    15061 taatttagag ttcaatgctt cagttgcact tgccatcggt acagatttca tcgtgatctt
    15121 tttcaattta ggcgtaccat tccagtagtg ctcgtttggc aagtattcta ctgattctcc
    15181 tgcaacgata ttactcatgt aataagggcc aaaagtcact ggatttttac gtacttgatc
    15241 gcttgattcc atatctttga ttgctacacc ttctaacgta tgttttggtg ctgcatatga
    15301 ccagatacca ccacccgatt gaagcatgct tgggtttact tctttatatt cgatttcaac
    15361 tgttttgtcg tctatttttt tgattccgga gattgtgtcg gcacttccat catgatattc
    15421 ttccatacct acgatgttac ggaaagtgtc gtcataacgg ataccagtgt aatctttatt
    15481 cccaatgatt tcgtaaggga aaatcacatc gtccgccgtt acatcttcac catctgacca
    15541 tttgacgttg tcttttactt tgattgtcgc ttttttcgta tcttcatcta gatcaagcgt
    15601 tgcagctccg tcgtctgtga ttctgaaatc gtcatcgttt gcaaatagcg gttcatgaga
    15661 aggagccata aaagctgcat catatgaatc ttggtaaaac tcccactgga acaatccttt
    15721 aaactgactg tccgttgcga ccgcagcttc taattcgccg tcttcgattg catcatcatt
    15781 attttttgtt gcaattggaa aagttaattc ctctttgtta ttgccgttgc ctccagagtt
    15841 gtttccagag gtatctgtac ctccgccccc gcatgctgca agaacgacag cagacatcag
    15901 cgctgcaaat ccaaaaatcg ttttcttcat tcctatttcc cccttcgctt ctatactagc
    15961 ctaaccgttg gcgcgcatct gcagaacgct tgaatgcttg tccaacataa ttgatactca
    16021 acatcaaaac taaaataagg attgatgcgg gtaaccagat ccattgccga ttcactaaga
    16081 cttctccgtt acttgcataa ccgatcaatg tacctaaact aggtacattt gccggcaagc
    16141 cgaaacctaa gaaagtcaaa gttgtctcga taccgatgtt tgccgcaaag ttcatcgtca
    16201 aattagtaat gattaatgaa cttagattcg gcatgatttc gcggaacatg attttaaaat
    16261 cattggttcc cattgttttt gaggcactga cataatctct tcttgcttca gataatgtct
    16321 tgcttcggaa taatcgggct ttagctaccc aataaaatgc actcatgatg aatataaagg
    16381 accagacatt gtatctggaa ataatcgata cgaagacgat aatgatcagc atgatcggta
    16441 gaatcatgat aaaatcaacg atccgcatca agatgttgtc aatgatccct ccgtagtagc
    16501 cagagatgat ccctaatcca accccgatga tcgaagtaag gatcgtgatc gcaaatccga
    16561 tcgtaacaga gtttctagca cctatgatca attgtcctaa tacatcacgt ccgccttcat
    16621 ctgctcccag aatgaacttt tgtccttgtg tcgaaactgt tcctggtgca gcgtatttgt
    16681 cgaagatact aacatacatc actttgtctt gatcggtcag catcgcaccg ataaagatca
    16741 ctaataataa aaccactagc aaacctaatg aaaaaatagc aattttatct tttttaaatt
    16801 ctcgagcaat cattcgtatc cccataggag gaatactttc ttgaactgct gtattgtttt
    16861 tttcactcat cgatccattc tcctttctga ttggttttat tgaatccgaa tacgcgggtc
    16921 aacgatactc atgatgatat ctgaaagtaa tgtccccact agcgtcgcga ttcctaaaat
    16981 caatactaag gatgtgatca cggtatagtc ccgttggccg atggaatcaa taaatagttt
    17041 tccgactccc ggataaccaa aaatcttttc gataactaca gaaccagcaa tcaacgaagt
    17101 gatttcatac cccatttgag aagccatcgg caacgctgca tttcggaaaa tatgtcgact
    17161 gaatacttta ttcgtcggta cccctttgga acgggctgtt ctgacaaaat cgagcgattt
    17221 cgaatcgatt acttcacttc gcagatattg gattgtgacg gcagtcgcca aaaatgcttg
    17281 ggtaattgat ggtaacagaa tatatttcaa acggtctatc cattgagtga taaatccacc
    17341 ttgcaaacct agtgtttgtg atccacttgt tgggaaccag cctaaacgat aaccaaaaac
    17401 aaatagcatg atcaagccga agataaataa aggaacagca aaactaaaga agttgtagat
    17461 tacgacaatc ttatccaaga ttgaattttg ataccggcca gcaagcatcc ccagaggcac
    17521 agcaatcaaa taagtaagga caactgtcaa gatcgataga tataaagtgt tgatgatccg
    17581 ttcgccgatc aagtcagcaa cagagcgttt gtataagaaa ctttgaccaa aatcgccttg
    17641 caaagcattt ccgatccaac ggaagtactg gacataccat gggtcattca agccagctgc
    17701 ttgtcgcatc tgttcgatca ctgccggatc ctgatttgga ttgatcaaac cggtaaatgg
    17761 atcccccggc atcgcttggg ctaataagaa gatcagaaca cttaaaataa taacttgcgg
    17821 gatcatcaat aaaacacgtc gcagaatcgt tttccacatt agcttgcacc tccatctttc
    17881 aacgccactt tgtgtgtagt tgtgatcgta cgcaaatcgt agactcgacc agtcgcatca
    17941 tagtattctt tttggttttg gatatactct tgttctactc ttcgtcgatt ttctttgtgg
    18001 ctttccctat tttctacatc gatttgcgga atagctgaaa gcagacgttt cgtatagata
    18061 tgtcgcgggt cattataaat gtcttctctt gtaccgattt ctacaaatcg acctttgtac
    18121 ataattgcga tattgtcgca catgtgtttt actacaccta aatcatgtga aatgaataaa
    18181 tagctgaggc cgtattcttg ctggattcgt ttcatgaaat tcaatacttg cgcttgaaca
    18241 gatagatcca gagcagatac aggttcatcg gctacgatca gttttggatt ggtagcaaca
    18301 gcacgggcta ctcctagacg ttgacgttgt ccgcctgaaa attcatgagg atatttatat
    18361 agcgcatctt ccggcatccc aacgatatct agcagtcctt ttaccttttt cttttcttct
    18421 tgatcgctta accgttcaaa atttcggatg ggttcagcaa tgatatccaa cactcgcttt
    18481 ttaggattca aactggacat tgaatcttgg aagatcatct gcacatcctt gttataaccc
    18541 atttttttgc ggttgcttcg cttcgttaca tcttttcctt catacatgat ttgtccggct
    18601 gttacctttt ctaatcccac aacggctttt cccgtagtag attttccaga gccagactct
    18661 cccactaaac catatgtctt tcctttttcg ataataaaat ccacaccatc tacagcatat
    18721 acatgatccg ttactcgatt gaagaatcca ctgcgaatag ggtaatgaac tttcaggtct
    18781 ttgattgtga ttaattctgt catctacacc tctccttttt cgtctctaaa gtggaaatgc
    18841 ttatagcagg agcaacgtac caagtggttc ggtgcgactt catgaaggat aggctcttct
    18901 tcatgatcgc ttgctggtat ccatggaata cgtggtgcaa accgacaacc tttacgaggc
    18961 atgtttttca gagaaggaac gacaccttcg atcacatgga gttctgcatc atgatttcct
    19021 tcttgtggta tagagttcag taaagagcgt gtataaggat gcttaggatg atcaaacagt
    19081 tcttttacac ctgctacttc aacgaactgt ccgccataca tcactgccac tcgatcagcc
    19141 atttctgcta cgactcctaa gtcatgagtg atcaagatga tccctgtttt tgtttcttcc
    19201 tgaaggtctt tcagtaaatc aagaatctgt gcttggatgg ttacatctag ggctgtcgtc
    19261 ggttcatctg caatgacgat tggaggtttg caggcgattg cgattgcgat gatgacacgt
    19321 tgtctcatac cgcctgatag ttcatgggga tattggcgtc caactctcgc aggattagga
    19381 atccctactt gttctagcaa ttctagtgca cgagcttgac gctgttctgc attcatcttt
    19441 gtgtgatagg aaagtccttc tttgatctgg tcttcgattc tcatgagagg atttaatgca
    19501 gacaacggat cttggaaaat cattccgata tcatttccgc ggattttatt atagagcgtt
    19561 tcgttcaggt cgattaaatt cagatcgtta tataaaatct cacctgtaat ttttgtgtta
    19621 ttaggatcaa gtaatcccat aatcgtagta gccaaggtac ttttcccgca cccagattcc
    19681 ccaacgattg ctaaaatctc gtttttgtct aaagtaaatg aaacgtcatc tactgcatcg
    19741 taatagtcat ctttgagtcg gaatcctgta tgtaggttct gaacttctaa taattggtta
    19801 tcggacaatc gaatacccct ctctgcttcc taaatggaag tttttcttaa taaaaaagga
    19861 cattacacag aataaagcgt tttataacaa aggttattct tatctttgtg taatgaatta
    19921 ttaataatta tatagaaaat cgctttactt tgcaacataa tttctgttta tcgccagaat
    19981 tatcagaaaa atgactttta cgtgtcaaaa agattaaaaa aagaaggata aaaagctgta
    20041 tgcagctttt tatccttctt tgcttttatt tgaaattatt taagatggct gctgatataa
    20101 tctactgcgc caccgactgt ttcaatttgt tcagcgtctt catctgaaat ttccgtaccg
    20161 aattcatctt caagctctaa aacgaattcc ataatactaa ttgaatcagc ttttaaatca
    20221 tctttgatgt tcaattcatc cgtcacttta tcggtatcta tctcaaaatg atttgaaata
    20281 attttcgcta ctttgttaaa tacttcttcg cgagtcaact ttttcacctc caagaatcat
    20341 acacttgtca attacctaca ccatggctaa ttttactggc atttttcagg aatgtccacc
    20401 tttttttcaa tggaacggat tgcttcagat atccaaatac tattcttcgc ttttttcaaa
    20461 ttgttcgact aattgtccaa ctacatctgt ctcaagcatt gtgtggattt ggcggatcgt
    20521 atagcgaaca gcatcggggc ctgttgctcc atgagtttta atgactggtg ctttcaagcc
    20581 aaacaaaacg gcaccgccat gtttggaata atccatttct gctttcaaag aacgtaagcc
    20641 gtcttttaaa agcatagcgc ccatttttcc tttgatccct tcatttagaa tagcagattt
    20701 taatagattc atcatattca ttgctgttcc ttcaatagat ttcaaaacag cgttgcctgt
    20761 aaatccatct gtgaccacta catcagccac accattgagt agctcacgag cttctacgtt
    20821 tccaataaaa ttcaagcttg tttcattctg aaggagttca taggcttttt tcgtcacttc
    20881 actacctttt gttgcttctg ttccgttgtt taagagtgcg acacgaggac gtccgatacc
    20941 tcggacttta cgtgcataaa aagaacctaa aataccgtat tgcaacaaat gttctggttt
    21001 gttttctgcg ttagcgccaa gatccagcat atcgaaaccg gcaccttctt tccctactac
    21061 tggcaaggta gacatcaagc cagggcgttc gatatttttg atacgtccta cgatgaacaa
    21121 ccctgctgct agtagcgcgc ctgtattccc tgctgaaaat acagcatctg cttcaccgtt
    21181 tttcactgct tgtgcagcaa ggaccattga tgcagtcttt tttcgtcgaa ttgcttttac
    21241 tggctcatca tcactattga ttttttcgtc tgtatggata atcgtgatgt ttttttcatt
    21301 tgttaagtat ttcttgattt ctgcttcttt tccgtaaagt aagaattcta cttctggaaa
    21361 atcttgctgc gctaaagcca cgccttctac aattgcttga ggtgcgtgat ctccgcccat
    21421 tgcatctacg gcaattttca ttgtttccac tcctttttcc gtcatcagta taaagtatag
    21481 cacagtctgc taatcgaaat aacgattatc ctgattttct atttggaggc tttgcgctaa
    21541 aggctgatac tcaggaagca tctgccaatc ctttatctgc caaagggctg cagcctcttc
    21601 tctggctacc tccaaaacat ttccatccgt caccaagtca gcaatcgcaa attgaggcag
    21661 accagattga cggaagccaa atacttcacc aggaccacgt aattccagat ctttttcgct
    21721 tagtacaaaa ccattggtgg tctctgtcat gattttcatc cgttccacac ctagttcatt
    21781 tttaggatta gcaactaaga tacagtagga agcatcactt ccgcgtccaa cacgtccacg
    21841 aagttgatgc agctgtgcca atccaaatcg atccgcatcc atgatcagca tgacggtcgc
    21901 gttcggcacg tttaccccta cttcaattac agtagtcgaa actaatatct gcatctggtt
    21961 gtctttgaag gcttccatga ttacttcttt ttcctgattc ttcattttac catgtaacaa
    22021 gccaacttca tattttggtg caaatagttc acgaagtttt tcatagattt ccactgcatt
    22081 ttttacatct aatgcttctg attcttcaat cagcggacag atgacataca tttgatgtcc
    22141 tcttgccagt tcttttttcg tccaatccag tacactgtcc aattgaggtg ttcttaccca
    22201 gcgtgtttcg atcgggatac gtccagctgg aagttcatca atgatagata catccatctc
    22261 accatatgct gtaatagcta atgttctagg aatcggagtc gctgtcataa ataagacatc
    22321 tggtcgccag cctttttcac gaagtacttt ccgctggttc acgccaaaac ggtgctgctc
    22381 atccgtaata actagaccta aattctgaaa ttcgactcca tcttggatca atgcatgtgt
    22441 gccgatcacg atgttaattt ccccttgctc tagtttttca aggagctcac gacgttcttt
    22501 tgctttcgtt gaccccgtaa gcaatgcgat ggttacttca ttgggatcaa acagctgttg
    22561 caagctttcc atatgttgtt gtgccaagat ttctgtaggc accatcaacg caccttggaa
    22621 acctgctgta acggtagcgt ataacgcaat cgcggcaacc actgtttttc cgctacctac
    22681 atctccctgc aaaagtcgct gcatatgctg cggactgcga agatcccgac agatttcatt
    22741 cgttacacgt ttttgtgcat ttgtcagttc aaaaggtagc ttttgagtaa attctttcaa
    22801 tttttggaca tcataaagga tctccaaacc attcgtttct gctttttctg cttttttcaa
    22861 tccttgaaga cgcatttgaa ataaaaagaa ctcttcaaaa acgacacgtc gtttggcttg
    22921 atggttttct tcgtggtctt ttggaaaatg catacttctt acagcagaag ctcggtcaag
    22981 cagtcgatat ttctccacaa ggttgtttgg cagattttct tcaatcaatg tgccgtattc
    23041 ttgaaaccct tttttgatta gatcaactaa tgtcgtttga cgaattgatt tgttgacgtg
    23101 atatatcggc gaaaagtcat ccattgtttg agaacctaaa atcttcatcc cgttcaatgc
    23161 tttacgcttt gcatcccatt taccgtatac ggcgatttcc tccgataaaa tgactttttc
    23221 ttttagataa ggttgattaa aaaaagaaac gttgaacaca tcatgttctt gcatcatacg
    23281 aaattgcaat cggctttttt tataaccaaa tcgattcatc acaggaggcg agactacgat
    23341 tcctttcaaa gtaacctttt cttgatcttg gatctcgttt agatttcttt cctgaatatc
    23401 ttcgtagcgg aaaggataat aagtcagtaa gtcttcaatc gtttggatgc ccaagctggc
    23461 aagactagct gctctttttt ctccgactcc tgccagcata gtgattggat catctaaaga
    23521 acgcataaat ttttcctccc tcccttaaca aaaaaaggac ttggtcagcg atcatacctg
    23581 atcactaacc gaaggccttt tatgaatgaa tactgtgtta gaaagatatt ctgacacaga
    23641 aattattcag cagaaaatag ataaggatag acaggctgat ctccttggtg gatctcgatt
    23701 tccaaatcag gatgtttttc tgttagtttc tcagcaaatt catttgcttg ttcaactgtt
    23761 ccttcatcac ctgccaagat cgttacgatc tctgtgtcct cattgatcat ttgttctagt
    23821 gtacttaacg ctgttgctgt gatatctggg ttagagatca ggatttttcc atcgatcatg
    23881 cccaaaaagt ctccttcttt gatctctaca ccgtcaattg aggtatctct gatcgcgtga
    23941 gtgacttgac cgctcacaac gctagacaac atttctgtca tcgtttcttt gttttcttct
    24001 aaagaagctt gatcattgaa tgaaagcata gctgtcaatc cttgggaaat cgttcttgaa
    24061 ggaacaactg ctactgggat atctgctact tctgctgctt gatcagctgc catgaagatg
    24121 ttcttattgt ttggcaagac aatcacttta tctgcgttta cttcttgaat cgctttaaca
    24181 atgtcttccg tacttggatt cattgtttgc ccaccactga taacataatt agcgcctaaa
    24241 ctttcaaaca gctcttggac gcctttacca gcagcgatcg ctacgatacc atacgggata
    24301 cgtggtgttt cttttttcgc aaactcgttc acttcttcat cgtgttccaa aatcgtctca
    24361 tgctgcaagc gcatgttgtc gactttgatt ttgactaatg aaccgaattt ttgtccataa
    24421 ttcaagactt ctcctgggtt ttctgtatgc acgtggactt tgatgatctc gtcatcattg
    24481 accacaagta aagaatctcc tataccatct aaatagttac ggaattcttc gtaatcgaat
    24541 gtactatcga ctgtaggtcc ttctccgatt tttaccatga tttctgtaca gtaaccaaaa
    24601 tggatatctt ctgtcgccat ttgaccttgg acactgcgat gatgttctgc attgaccata
    24661 tcatccattt ctgctggaga aggttcatat gtttcatccg cttggaattc tccattcaat
    24721 gcacttaaaa agccctcata aacaaacagc agtccttgcc ctccactgtc tacgacaccg
    24781 acttctttta ggactggaag caagtctgga gttttagcta aagcacgctt tgcccctttt
    24841 acaacagctg tcattacttc aacgacatca tcagtacttt ttgctttctt ttctccagct
    24901 ttcgctgctt ctctagctac tgtcaaaatc gttccttcta ctggtttcat tactgcttta
    24961 tacgcagttt caactccatg agtgaaggct gcagcaagat cggaagcatt taatgagaca
    25021 acatctggaa tttgcttaga aaatccacga aacaattgag acaaaataac gccggaattt
    25081 ccgcgcgcac ccatcagcaa tccttttgaa agcattgctg ctaattctcc tactttttct
    25141 gaggttgaat ctactactgc cttagctcca ctagtcatag atagattcat atttgttccc
    25201 gtatcgccat ctggcacagg gaatacattt aatgaattga cgtattctgc atttacttgc
    25261 agacgattcg cacctgcctg gaccatttcc tggaactggc ttgcacttat ttctgttaca
    25321 ctcacctaga taatcctcct tcagatcgac tgctgagtta ccagccagtc tcctgcctag
    25381 tctggcagta cacgaacacc ttgtacaaag acattaactg aattggcagt tacgcctaat
    25441 aatgcctcta gattgtattt cactttttct tggacgttgc gggaaacttc agagattttc
    25501 gtaccatagc tgataatcgt ataaacatct acagcaatac cattatcttc ttggcggaca
    25561 actactcctc ttgagtaatt ttctttacgc aaaatctcag taatattatc tttaatttga
    25621 tttttgctgg ccattcctac aacaccgaac acatctgtcg cggcgccacc aacaactgtt
    25681 gcaattacat cattggtaat ttcaatggtt ccagctgacg tttgaatttt tacagccatg
    25741 aatgagcctc cttaacgggt aaaaaactcc catttttctt ctatcatatt ttatcatagc
    25801 gcaacttgaa ataaaaggca gttacccaaa gtactcaaaa accttgcaaa atttcaggta
    25861 taaccttgtt taatttacaa ttattcttct caattgtcaa ttgtatctat gaaaatgtac
    25921 ttgcaaattt ccattctata tgatatgtta gtctggtatg agctgaatta ttctattgct
    25981 ccaaatcaag gcaaaggagg aaattaccat ggcaaaagtt tgttacttta caggtcgcaa
    26041 gacaaaaagt ggaaacaacc gttcacacgc tatgaactca acaaaacgta ctgttaaacc
    26101 taacttacaa aaagttcgcg ttatggtcga cggaaaacct aaaaaagttt gggtgtcaac
    26161 tcgtgctttg aaatcaggaa aagttgaacg cgtttaattg aatgatactt ggaccgttca
    26221 gatagtgaac ggttttt
//
LOCUS       DAYYAR010000024        25409 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000024 DAYYAR010000000
VERSION     DAYYAR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.024"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..856
                     /locus_tag="ACHAT0_000901"
     CDS             8..856
                     /locus_tag="ACHAT0_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8450863.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1027..1368)
                     /locus_tag="ACHAT0_000902"
     CDS             complement(1027..1368)
                     /locus_tag="ACHAT0_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFM8450864.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1454..2161
                     /locus_tag="ACHAT0_000903"
     CDS             1454..2161
                     /locus_tag="ACHAT0_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8450865.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2148..2471
                     /locus_tag="ACHAT0_000904"
     CDS             2148..2471
                     /locus_tag="ACHAT0_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8450866.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2560..3597)
                     /locus_tag="ACHAT0_000905"
     CDS             complement(2560..3597)
                     /locus_tag="ACHAT0_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8450867.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4044..5027)
                     /locus_tag="ACHAT0_000906"
     CDS             complement(4044..5027)
                     /locus_tag="ACHAT0_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8450868.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5443..6213)
                     /locus_tag="ACHAT0_000907"
     CDS             complement(5443..6213)
                     /locus_tag="ACHAT0_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8450869.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6206..7141)
                     /locus_tag="ACHAT0_000908"
     CDS             complement(6206..7141)
                     /locus_tag="ACHAT0_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8450870.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7735..9384)
                     /locus_tag="ACHAT0_000909"
     CDS             complement(7735..9384)
                     /locus_tag="ACHAT0_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8450871.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9579..10475)
                     /locus_tag="ACHAT0_000910"
     CDS             complement(9579..10475)
                     /locus_tag="ACHAT0_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8450872.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10468..11148)
                     /locus_tag="ACHAT0_000911"
     CDS             complement(10468..11148)
                     /locus_tag="ACHAT0_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8450873.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11149..11931)
                     /locus_tag="ACHAT0_000912"
     CDS             complement(11149..11931)
                     /locus_tag="ACHAT0_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8450874.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(11924..12841)
                     /locus_tag="ACHAT0_000913"
     CDS             complement(11924..12841)
                     /locus_tag="ACHAT0_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8450875.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(12844..13128)
                     /locus_tag="ACHAT0_000914"
     CDS             complement(12844..13128)
                     /locus_tag="ACHAT0_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450876.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            13916..17143
                     /gene="fss3"
                     /locus_tag="ACHAT0_000915"
     CDS             13916..17143
                     /gene="fss3"
                     /locus_tag="ACHAT0_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8450877.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            17255..17569
                     /locus_tag="ACHAT0_000916"
     CDS             17255..17569
                     /locus_tag="ACHAT0_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8450878.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17582..17956
                     /locus_tag="ACHAT0_000917"
     CDS             17582..17956
                     /locus_tag="ACHAT0_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8450879.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            17957..18310
                     /locus_tag="ACHAT0_000918"
     CDS             17957..18310
                     /locus_tag="ACHAT0_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8450880.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            18379..19395
                     /locus_tag="ACHAT0_000919"
     CDS             18379..19395
                     /locus_tag="ACHAT0_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8450881.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            19989..21863
                     /gene="ltrA"
                     /locus_tag="ACHAT0_000920"
     CDS             19989..21863
                     /gene="ltrA"
                     /locus_tag="ACHAT0_000920"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8450882.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            21965..22345
                     /locus_tag="ACHAT0_000921"
                     /pseudo
     CDS             21965..22345
                     /locus_tag="ACHAT0_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            22390..23532
                     /gene="dcm"
                     /locus_tag="ACHAT0_000922"
     CDS             22390..23532
                     /gene="dcm"
                     /locus_tag="ACHAT0_000922"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFM8450883.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            23522..23811
                     /locus_tag="ACHAT0_000923"
                     /pseudo
     CDS             23522..23811
                     /locus_tag="ACHAT0_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24067..25251
                     /gene="mobT"
                     /locus_tag="ACHAT0_000924"
     CDS             24067..25251
                     /gene="mobT"
                     /locus_tag="ACHAT0_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8450884.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            25248..25361
                     /locus_tag="ACHAT0_000925"
     CDS             25248..25361
                     /locus_tag="ACHAT0_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8450885.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         7953 a         4953 c         5005 g         7498 t
ORIGIN      
        1 gaactagatg aataattggc ttgagtattt tcctgaaaac gttttggaac gcggttacag
       61 ctatcatctg cacggttttg tccgtcattt gaactataca agcaagtact tgtccgcaac
      121 agtttctggc acggaagatt ataaggtagt gatcacttgg gatgaaaaga ccaacatgac
      181 ttgtgattgt ctatatgcca tagaaggaaa aaaatgcaaa cacatggctg ctgtcttatt
      241 cgcttacgaa gaacgtccaa taaaaaaatc caactactca ctttccgaac tctcgtccct
      301 tgtttcctcc gctagttcct ctcttgtacg ggagctttta acagagattc ttatagaaca
      361 tccgcacttt atcgaacgct tcaaagttaa gatgccattt catgcaataa attattcaga
      421 taaattaaca actatcatcc ataaatacga tcatattatc aagaaaaaca aaaacaggaa
      481 aacagcgaag ttcatcatgg agatgcgaaa gttcatccaa gaagcagttg aatcgcttat
      541 tcagcaaaat gcctatcttc ccgcattcga attgatcaat gaagtgattg ctactctcga
      601 aacattctat tgggagcctg aggatgaacg aacccttctt ctgatagagg actgttatta
      661 tttatggaaa gaattattag cagaagcacc tcatgcagaa aaaaggcaaa tgttctcttg
      721 gttcgtctgc caagtcgatc atactgacgc ctcttattca aaacgctact ctataaaaat
      781 cctgaaagaa gactttcggg aaaaagaatt ttctaaccaa aagaaaaaaa tagataaaaa
      841 actaaagaaa cggtaaagaa agacgatttc aatgaaaaat agcaagtaaa cgataacaac
      901 catcctaaat ccacaagaaa tacagcagac cttgctttaa aaacacgaat catgcaaagg
      961 atgaagtaaa gaagacttaa cgcgtaccca atattagtca tttgactatt aggtacgcgt
     1021 tttgttttat aagtcatgac aaatttcctt cgttgctgga tagattttcc ggcattgttg
     1081 atagatttta tgatactttt ctacattttg taagagaggt tccactgcat ctgtatattg
     1141 gataaaattc tgacaacaac tttcaggtgt gtcgaaccac cctaacccta gtgcagcaat
     1201 cattgttgcg cctaatgcag gtccttcctc cacacttaaa caatagaccg ttgcatcgaa
     1261 aatatcagct tggatttgta gccatgcaag atttttcgcg cctcctccca ccgaaacaat
     1321 acgtgtaaat ctgcgacccg ctagttcttt acgtttcaat cccaccattg acaaatgctc
     1381 attaaaaatt tagaatattc acaagtttaa cgacaattat gaaaatttat gtcgtgctaa
     1441 aggagttttg ttattggatg aaaagttaga tataaaaact gcgatcaaag acacgttacc
     1501 taccgttttc ggttatatcg gtattggact tgcatttggt atcgttggga aagctgccgg
     1561 atttcatcca ctagtcgtca cgttgatgtc cctgctggtc tatgctggtt ctgcccaatt
     1621 tatcacagtc agcatgcttg ctagtcacag cccattgctt tccatcgttt tctcgacctt
     1681 tctagtcaat tcccgaatga ttctgatgag tatgacgatt gctccttatt tcaagaaaaa
     1741 tcggctgctt caaaatctgc tgattggcac attgctgaca gatgaaagtt tcgcactggg
     1801 aatgaataaa ttgaactata ctggacaaaa gttaaacttt cgttggatga atacagctaa
     1861 ttggatttct tatttgacgt gggtgggttc ttctcttgtt ggcgcacttt tagggaattt
     1921 tattactgat ccgaaaaagt ttggtttgga tttcgccatc gtcgctatgt ttatcgggtt
     1981 actttatctt caagtcattt ctgatagaaa cacctctaaa cgattacagt tgatcctgat
     2041 tggtctcact ttgattctgg tatatgtcgg actgattttt atcccaagca acttagtgat
     2101 tgtagttgta acactgattg ggtgcggatt gggggtgtgg atcaaacatg ccttcttttg
     2161 agtttacatt gctgactatt ttaggttgta ctttagcaac ttggctttct agggtcctac
     2221 catttgtctt actgaaaaaa ttcgatttac cgcaaccgtt gcttgagtat ttaagttttg
     2281 tcccgatcgt cattatgtct gctttatggt tcagcagtct atttacccaa aatatcggac
     2341 atctgccgca gatcaatctc gaaaatgctt tggcttcagt tcctacactt ttagctgcta
     2401 ttctttcaaa aagcttattg gtgatcgtct tggcaggaat tctttccctt tcgttgattc
     2461 gcttattata aataaaaaaa caagagctta tggcaaatta ctgtcatgcg ctcttgtttt
     2521 tatcaaagtc aaataagaaa aaaatcggcg tattcttgct tatccgttga acggaccact
     2581 aacaacgact tttcctaaca tatgtcctgt ttctacgtct ttggttgctt gtttcaagtt
     2641 cttcgcattc atcccatctg aatagacctt gctcaatgta ctgcggataa tcccttcatt
     2701 tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt tcgatttcat agttatagtc
     2761 tgttttagcg aacatatact cccagtcgaa gctgatagat tggtttttcc aaacacctat
     2821 atctaacggc tctttgatcc ctacgatcgt tcccacatgt ccaaatggtc ggacaaggtg
     2881 cttgatttga tcaaagtagc gggtaatatc gaataataca gcgacatact ctacttgatc
     2941 gattcctaat tcacgaaaac ttttacttaa gtcgtgatga taatcgattg gatagtctac
     3001 acctgttttt ttcagccatt caaaattctt cggacttgca gtcgcgtaga cttctaggcc
     3061 agcccaatgc gctagttgat tcaaaatcga accgacgccc ccggatccgt tgatcactaa
     3121 gatttttttc ccgcgattgg cattttcttc tggagttaga ccaaattttt caaataacaa
     3181 ttcataagcg gtcaatgagg tcaacggcaa ggctgcagct tcttcatctg aaagattttt
     3241 tggtgcaaga gcgacaatac gttcgtcaac tagttggtat tcttgattgc ttcctgctct
     3301 tgttgttgta cctgcgtaaa aaacgcgatc gccgacagaa aatttttgca cttcgtcacc
     3361 tacagcaaca actttcccga ctccatcaaa acctaagata cgtaaagcat ttcttatccc
     3421 gttatcttgg cgaagtttcg tatcgacagg attaacagat actgcgttga cttttaccag
     3481 aagatcccgt gaactgggta caggtatctg ttctttttca tcaataaagc tttttggatc
     3541 atcgatcggc aacccttcat aaaaaccgac taccttcatt aattcctttt ctgacatacg
     3601 aattcctcct tagcttcatc aagttggagc atccatacag tgttcatttc aatatatata
     3661 gtatgctatt tgttccacct tttttattca tcataattat tctcttactt aaagtaaact
     3721 atttgcactt ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca acctcttata
     3781 aacggtgttc tcctaccaac tcttcggtca caagtcaaaa tattcatcaa acatgagaag
     3841 ctgttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctgat
     3901 ataaacggtg ttcaatcaac tgaccctcgg tattagctca aaaaacgaca aaatatgaag
     3961 agctattttt gtttggctgt tcttattact tgatacagga cagctttttc acgacctcta
     4021 attcacggtg ttcaagcaag aaatcattgt tcactgaaaa gcgttggatg ttctttttca
     4081 aaggtctcta agatctctcg tacttcatca gtagatttcg tattcatcaa ctgaactcgc
     4141 aagtcatttg ctcctggaaa tcctttgata tagattttaa agaaacgatg tagtcctgtg
     4201 atggaacgcg ggatgatttc tgaataatga tcttgcagat ctagttgcag acgaagtagt
     4261 ccgatcagtt ctttttctgt atgttccctc gggtcttttt caaatgcata aggattcttg
     4321 aaaatccctc ggccaatcat gatgccatca acgccatatt tctcggccaa ttcttctcct
     4381 gtctggcgat cggggatatc tccattgatc gtgattagtg tttgtggagc gatttggtca
     4441 cgtaaagcaa tgattttagg aatgacttcc caatgtgcat ctactttact catctcttta
     4501 cgcgtacgca aatggacaga aagattggct atatcttgat gaagtaggtg actgatccat
     4561 tcttccatct cagacatttc cgtatagccg atacgcgtct tgacgctgac aggcaaaccg
     4621 ccagctttag ctgcttcaat caattgtgcc gctacttctg gacgaaggat caagccactg
     4681 ccttttccac gttctgctac attagggact gggcagccca tgttgatatc gattccttgg
     4741 aagcccattt ctgccatagc aatgctcatt tctctaaaaa attctggctt gtctccccaa
     4801 atatgggcaa ccatcggctg ttcatcttct gtaaatgtta atcttcctcg tacactgtct
     4861 tttccatctg gatgacagaa actgtctgag tttgtaaact cagtaaagaa gacatccggt
     4921 gctgctgcat gctttacgac atggcgaaat acaacatctg ttacatcttc cattggtgct
     4981 aaaacaaaaa aaggctttgg cagctctttc caaaaattag tagtcattta tttgttactc
     5041 tccattctat caagcagtga agtcgcaatc tttctcaaaa acattgtcat tatactagga
     5101 aacaaaaaaa gaagcaaagt gaacagctta aaagatgttt ctgattgagc aaaaaaaaga
     5161 ctgtgacaaa aatcttatca cagtccattt tccggaataa atggtgttca aaagcttgca
     5221 tctgcggtaa taagtccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc
     5281 ttattacttg atgcagaaca gctttttcac aacctctcca ctaaacggtg ttcaatcagc
     5341 tgaccctcgg tattcgctca aaaaacaaca aaatatgagg aactattttc ccgtttgttt
     5401 ctctaatact caggtcaaaa cgctgatttc acaacctctt ttctaacttc tcaatcctct
     5461 tcctttattg atcaaatacc aagtaacggc gtacaaaaca acgatgaata acacaagtac
     5521 gatcatcgat gttgctactg gcacatcgat cgttccaagg aatccgtaac ggaaaccgga
     5581 gatcatataa acgatcggat tgacttttga aacagcttgc caaaagggcg gtaacataga
     5641 gatggcatag aaaacgccac ctagataagt taacggctgc aagacaaatg taggcacgat
     5701 cgatacatca tcataagact gagcaaaaat cccgttgagc agtcctgcta atgaaaacaa
     5761 gattgccgtc atcaataatg taatgatgac catgaaccat gaatacacat gaagtggtac
     5821 aaagaacaag gaaatgatcg ttacgagaga gccgacgaga atactgcgac ccacaccacc
     5881 aatcacaaag ccccaaataa tcacatgcgt aggaacaggt gctaccaaga tttcttcgat
     5941 atttttctga aatttttgag agaagaagga cgaagaaaca ttggcatacg aactagtgat
     6001 agcagacatc ataatcaaac caggaacgat gaattccata tatgaaaaac cacccatgtc
     6061 accgatccgc ccaccgatca tttttccgaa aataacaaaa tacaaggatg tcgtgatcac
     6121 tggcggaacc agtgtttgga cccatatacg caaatagcgg ttcgtttcct tgatagctaa
     6181 actttttaat gcagtaaaat aaagattaaa catttttctc ctccgtatga ttattttctt
     6241 ctgtgatttt caagaacagt tcttctaaac gattcgactt attcctcata gaaagaactt
     6301 tgattccttg tcttgtaagc tgatcgaaga ttccgttcac cccttgattt cgttcaactt
     6361 cgacagcgat tgtctgctca tcttcaaatg tacaagcata tccttcaatc acaggtttgg
     6421 tatcatatgg cgctaaatca aagataaatg tttcaaattg cagttttgac agaagctctt
     6481 tcatacttgt attctcgatc agttcacccg attggatgat tccgatgttg cgacaaagca
     6541 tttcagcttc ttctagataa tgcgtcgtca aaatgatcgt tgtaccattt tcgttcaatt
     6601 ctcgtagaaa ctcccacatc tcgcgtctca actcgatgtc aactcctgct gttggttcat
     6661 ccaagatcag caaacgtggt tcatgcatca atgccctagc aatcatcagt cttcgcttca
     6721 ttcccccgga aagcatacgt gcacggacat ttctcttttc ccaaagattg gactgcttca
     6781 aatatttttc gctgcgtttc atcgcttctt tgcgagagac accgtagtag cctgcttgat
     6841 tcacgacgat ttgctggacc gtttcaaaag ggttaaaatt gaactcttgt ggaaccaatc
     6901 caatctgttg ctttgctcga actagatcct tgtccagatc ataaccgaag acattcactt
     6961 ttccagatgt tttattgact aaagaagtaa taatcccgat cgttgtagac ttcccagctc
     7021 catttggccc taatagcgca tagaaatctc cttctttcac cgaaagatcg attccccgca
     7081 atgcttcgac cccacttcca tagatctttt tcaaattatt tatttcaagc gcatttgcca
     7141 tttatttcct atctccttat agacatgatt cttaattgta tcatggaata cagcagatga
     7201 aaaaacaact tggcttccac acaaaaacac acctgctaac aaagaggaga tgtgttttct
     7261 agttcatgat catcttcgat agaataacta tctaaacaac tctggaactt ttaaaatcat
     7321 aaaagtaccg acagcaacta ctacgatgac atatagaaga tccttataac tgaggttctt
     7381 gtctttttta ttcatgttaa gctttcctcc gtccttttat cgaaagcttt tttcttttat
     7441 tttaaccgaa ataaataaaa tggaaatagt tattttcaat ttctgttttc gctgtttgtt
     7501 ttacggagaa ctttgccatg gaacgtgcgt ctcgtcgctg atgctaagtt ttttcgataa
     7561 aagctgctag aaggaagaca gcgtggttta ttttgtatcg tcttttgcta aatcttccaa
     7621 gatcatctgg acagcagact cgttcccatt gaattctgcc acttttttgg catcaatgta
     7681 cttcttttga taatcgtctt tttggaccca cgcatcatgc ttcgtttgat aaagttaatc
     7741 aaacggatac cactgacgct gttcatcgaa caacaaactt tctttttggg cctgttcaac
     7801 tatggattta ggataattca aaactttcag caaggcaatc gcatttcggg tccttgaagg
     7861 tccttctttt aatttgaaat caaaactgat tccttttcct tctgttactt gttcttcaaa
     7921 gtggacattc tcacaataat tctttaaaat ctctgtcaat tcgatatcat gtgtggcgac
     7981 aaatgctaaa gatggatact ccgcaagcca ccgaacgact gaagcggaag aagcaatccg
     8041 ttcgatcgta tttgttcctt ttaaaatctc atctacgaag cagtagcagc gttctttgga
     8101 agccacttgg tccagcaaac gtttgatcga tttgatttct gaaacaaaat aactgtcgcc
     8161 ttcaaataaa tcatcttcga ttgccatgga agtcaacaca tgtccaggtt gcagggtaaa
     8221 ctgttctgca atcgctgttt ggatcgtctg tgctagaata cagctgatgg ctatactttt
     8281 gacatacgta gattttcctg atgcattgga acccgtgact aatgtattct tctgccagtt
     8341 gactggatta actaccgctt cttttaacaa tggatgatag atatctttcg ctgtcacacc
     8401 gccctcagta aactctggtc ggcaagtgat cggcatatag gtgcgataat tcaaaacggc
     8461 tgctgcaact tccaattctc ccagcaattc ccacagcttg atggcttcag ttgaatgctt
     8521 ttctaattta gtgagaacga aatgatagga gataaatggc agcatgaaca tgatgttgac
     8581 atattcaaaa atcaattctg cttcactccc gctcttcatt cgaaatgaaa aacctaattt
     8641 agggatgtct cttaatggtt tagtttgctg tttcagctct ttttgaagtg gtgtcgtcat
     8701 ttttgctaac tgattggcgg ctgaaatcgt tgaaaccaaa taactcatgc tattcagctc
     8761 tgtttctagt gtttgtttct tcactagata atagaccgta ttgaatacta agccaccgat
     8821 tgctagatag attcctagta cttgccctaa aagaagcaaa cctacaccca ccagcggaag
     8881 caagccaaga aaaataaata aacctacgcg tccaatcttt ttactttttc catccgctag
     8941 atattgtttt gcaaaattat gatctttttt tcctaaacga gcgaattgat attggattct
     9001 ttctctcagt tgaggatttt cctgataaaa agcaattagt ttttctaact gataatcttc
     9061 tccaaaatca aagctacgta aacgctgata caacgcttct gaacctacac tagaataggt
     9121 actgttgatc ccttcaaata cttcgaacat atccaagtca taccaagtaa gatcgtcgat
     9181 ttcactatcc catgaacgga attttttctc cgtgagccac gcatccttca aactttcttc
     9241 tttatcaaat ctcggttgat aaggaatttt tccccaatta ctacgaactg tttcctttac
     9301 tttcaaacgt gaaaaaatgt ctaaaacaac taataaaata acaactgcaa taataattaa
     9361 aacgataaat tctctttggc ccatatccct ctccgttcct tgatttgtta gttttagtat
     9421 agcactgttg cgctatttga aaagggggta caaaaataac tgtcttcgtt acctaattcg
     9481 tatcaatcac accaaaataa aaacaccttc caaaaaagaa gatgtcttta aaacgagata
     9541 caattttttg tcattcactt caggtaaatt agagccaatt aaaccctccg caacttgacg
     9601 gagatggaga agatgccccc gtctaatcta gcggtcatct ctcctcccat tccttccacc
     9661 aatttttttg acaggtaaag cccgattccg ctagattccg tatttgtgcg gctggtattt
     9721 tcggtataaa aacgctggaa gactttttca acagaggttt gcgctcctgg tttgatagga
     9781 ttggccactg ttaatcgaat gaatgaatcc tcttttgaaa atgtcacctt tgcttgtcca
     9841 tctgcataac gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa catgagcgga
     9901 tctgctaata cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat atttttctca
     9961 gaaaattgtt cataaaaaga gaaaaatact tcttccacct ctttagaaaa agaaagcgta
    10021 tggagatcca tttctttttg cttcgtctct attcgagcaa gatcataaaa ctgatccgtc
    10081 atactgagta atcttgcaac agattgtttg atgatcgtta aatacttctc ccgtttctcc
    10141 gtatcctcta cctgttccag taattgtaca taaccgttaa tagcagtcaa aggagttctg
    10201 agatcatgag aaaggccgac gatcgatagt ctgatgtttt cttccatttc ttgaaatgct
    10261 tgtccttttt gttcattttc atccacccat gtattcagtg catctactag gtccattaga
    10321 ttcttttctc gaaattcgac tgaaagacga ccaccatatt cttgtcgttc agaaagagtg
    10381 gcgattttct tagttaatct cttgatttcc ctttgcagga agaaaataaa acttcccata
    10441 aagataacaa cgagaagcaa cagccactta atcaaatcta aaccccactc cccacaccgt
    10501 atgaatatac acagcatgtg cctttgctag tttccctcga agattggaaa tatgcatatt
    10561 cactgttttc tcactatcaa aatatggttc ttgccaaact gtttcataaa gatttgctcg
    10621 cgaaaatact tttttcggat gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt
    10681 caaatggaca gccacaccat tcacatttac atccctcgac tctttgtcca gtaagatttc
    10741 cttgtaatag atgcgttgcg acagatcttt tacttggaca gaagtctgat gacgaagttg
    10801 gatgccgatt cgtaacaaca gttcttcgat atcaaatggt ttcgtcacat agtcatccgc
    10861 tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa gaacgatgat
    10921 cggaacattc gatgtctgtc ggatttctgc taacaatgct tcacctgaaa gccctggaag
    10981 catgagatct agaaggatca aatcgacagg atatgtgctt aacaataatt ttccttccgt
    11041 acctgaataa gcatcaagaa gtttgtatct ctgttcaaga acctcctcca gtaaagaatg
    11101 aacagcttca tcgtcttcga taatcaaaat agtttccagc aggaacacct acttaatgtc
    11161 agatttttgg aaaataaaca ttcctagagc tgatgatacc acgattgtta ctagactgac
    11221 cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg tatacatgag
    11281 ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc
    11341 gggaacgaag gccgttccca tgatataaag aaaagaaaga agcaaactga cactggtact
    11401 tctagtgatg aatgtcaaag ccactggaac agataatgtc gctaatatca gcagaagcaa
    11461 tacaggaaat acttttagaa cataatttcc atagtttttg attccctcaa cttcaccaaa
    11521 tccgaaagca agtgtgtaag caatcatggc tagccccgta atcaaaacca cgccaagtgt
    11581 tgtgatcaaa agcgtaacga gaagtttcga cacataataa gtcattcgat tccgtcctaa
    11641 agataacgta ttgcgaatcg taccgttttg aaattcttcc ccaatataaa agcacgcaaa
    11701 tgctgcgaaa gccagaagaa tccatgcagc cattttttcc accattaaaa tccctgttgc
    11761 accgttgatg actgtatccg ttgcttggtt taattcattg ccgtattttg tatgcatata
    11821 ccattcgttc caagaataaa aaagcggaag tacgaccatc agtcctagtg agacccacat
    11881 tttacggctc ttccatactt ttattttttc agcagttaat agattaaaca tgtggattgc
    11941 ctccagttaa ttgtaagaaa taatcttcca gtttttgccc aactaggtgg ataccatcca
    12001 caggaacttt tgcctctacc aatgcttgat tgatggctgc tacttggtca attccttcaa
    12061 aaagctggat aacacggcta gattggacaa aataatccct ataaccaaga gagtcgagaa
    12121 ccgttactgc ttgttccgtc gccgaagtgt caatttttat aaattgacga gattcttgtg
    12181 ccaattcttc tttcgataat tctttgatca agtgtccttc atgcaaaata ccgtagtgag
    12241 tggcgatctg tgacaattca tctagcaagt ggctagaaag caagacggtg atgccccgtt
    12301 cttttaccaa acgttggatg atttctcgca tttctgccat ccctgctgga tccatcccat
    12361 tcgtcggttc atccaaaatc aaaaattctg gattcgtgat cagtgcattt gcaatagcta
    12421 aacgctggcg catacctaaa gagaaattct ttgctttctt tttccctgta ttttccaatc
    12481 gcatcatttt cagcaggtct tcgatttctc tatccgacac accgccgttc gctgcttgga
    12541 tacgtaggtt atctcttgca gttaattctg gataaagagc aggggtttca atcgattgac
    12601 ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc gaaccttcat
    12661 caatcgagat caaacccata atagttctca taaaggtaga cttacccgct ccattctccc
    12721 caatcaaacc atagatcatt ccacgtttga tttcgatcga tactttatct aatgctttgg
    12781 cagcaccgta ttttttggtt ataccagttg ctttcaagac cgtttcattt ccaaaatcca
    12841 tacttatcct tctttcttat tgataagtga agtatagttg acgggtataa agaaagcagc
    12901 cagaaatgta taaagaaagt gtaaattttc caattgaatt tcaagaaaaa tcagactgtc
    12961 tcgcagacgt ggttgagttg tctgccaatc aaagagcgca ccaaacagtg ctaatccaag
    13021 tggactggcc accatggtaa tcgttgttat cgttgacatc agacgtcctt gtatatgcgt
    13081 tggaacttct cgctgaagcc agaccatata aaaagcaaaa gaaaacatta atccttaaaa
    13141 acggacttat gaaaaataat acatgccgtt tgggatttac aattagctac caaataagag
    13201 gttatgacaa aagacttttc acaatccctt cttccgaata aacggtgttc tcctgccaac
    13261 tcctcggtca caagtcacaa tatttatcaa acatgagaag caatttttgt aaattccttc
    13321 ttatttcttg aagctgacca cttctgtcac aacctcagaa gcggataaat aatattgttt
    13381 gatgatactt tatctttttc ccgtttgttt atagatcgaa tgatattata gaccaagaat
    13441 cctacctatt aacatagcta aaaacacaaa aaaggcactt tccaaattgt ggaaagtgcc
    13501 agaaaagtta ggattaaggt aactgcgctc agcattgctt cgcccagtac gattctcgta
    13561 actcgtgaac tcgaacgata atcgcaatta acgttttgag aactgtgaag ctttacgggc
    13621 tttcttaaga cctggttttt tcttcgtatc attgaataag tttgaaaagc cttgatttat
    13681 aaaggttttt ccgtaaatat attttgttat atttgattaa atttgaaatg tttgtgttct
    13741 aaaatgtgtt ctaatttaaa ggcagtatat ttcgtatttt atattaaatt tttaggctca
    13801 ttcattctca aatccattct atttagtcaa taaatatttt aaaaagactt tataaacaaa
    13861 gatgttcaag cgagcatctt atttttttgt gcctaatttg aaaggagaac atttcatgta
    13921 taaaaaacaa aaaaagaaat tatgtgtatt aactattttg agtttggctg ttcaatcaat
    13981 tgtaggagca attgctccta cgattgcttt tgctgatgaa attacccatc cacaaacggt
    14041 aaccgtagaa ctagatttag cacatcaata tgctgttgaa ggaactttta gtgatggtcg
    14101 tccaatgtca gaagtcactg ttccgcatta tgcagtttac aatggtgtga agcaagatat
    14161 tttctgtgtt gaaccaggag tcccgattta caatgagttc actcctggat atgagaagaa
    14221 cccgttacca gatatgtccg ataaagccaa attagtttcc gttttatgga aaaaagtagg
    14281 tacagatgca gatactcata ttgtggcaca gaagatgatc tggcaagaag ttaatgggta
    14341 tacccttcac tcaataaaac gatcagatgg tagtgccgta aatatcgcag ccattgaggc
    14401 aaaaatcaat caagccattg ccgattatca gaagaaacca agcttccata atagtacggc
    14461 taaaacagta ttagggcaat cgactactgt gacggatacg aataatctga atttgtcaga
    14521 gttcgatgag gttgtggaaa ataccgcaaa cattgattat cgtgtgaatg gcaatcagtt
    14581 gataatcagc ccaaatgcca attcaaaaga aagtggtgtg ttgacgctta agaaatctgc
    14641 tggtaccgga acacctgtcg cctataaaaa ggctggccaa cagactttaa tggccggggc
    14701 gatcgataag ccgaacactt atacggtcaa aattgatgtg gaaatggagg gttctttgaa
    14761 gattaaaaaa gttgataaag aatcgggtga tattatacca ggaacggttt tccatttaga
    14821 ctttggaaaa actttacccg caaaagacgt gaccaccgat aaagaaggca ttgcgacctt
    14881 agatgagatt cctcatggta caaaggtaac cattactgaa aaatcggtgc cggcgcctta
    14941 tacgattgat accacaccca tgactgccac aattaaagca ggtgaaacca tttcagtgac
    15001 ttcaaaaaat acgcgagaaa aaggtcaaat tattctggat aaaaccggaa ttgaaacagg
    15061 gagtgatctt tggaacgata attactcttt agcgggcaat acgtttgcca ttcgcaagga
    15121 tagcccaact ggtgaaatcg tccaagaaat gaccacggat gaaaaaggtc atgcagaaac
    15181 accaaaagag attgccaatg cgttggaatt gggaacctac tatgtgactg aaaccaaggc
    15241 tagtcatggc tttgtgaata ctttcaaacc agtaaaagtc gaattgaatt atgtcaatca
    15301 aacagtggct cttgttacca gtaacgtaaa agggcaaaac caagaaatta ctggtgaaac
    15361 cactttgaca aaagaagaca aagataccgg tcatgaaact caagggaaag ctgagtttaa
    15421 aagagctgaa tacaccttgt ttactgttaa ggatggaaaa gcggtcaaat ggaccgagaa
    15481 ctttaaacca gaattgttga agggaacgaa agcttctgat gaaaccgtaa ctttgacttt
    15541 agatgaaaag aatcaagttg ccgttaaaca cttagcgatt aatgagtatt actggcaaga
    15601 aaccaaagcg cctgaaggat ataccttgga tgaaacaaag tatcctgtat ccatcaaaaa
    15661 ggtggatgat agcgaaaaaa atgctgtgat tactcgagat gttgcggcaa aagaacaagt
    15721 gattcgcttt ggctttgatt tctttaaatt tgctggatca actactggca ctgccgaaac
    15781 tggcttcaat gatctaacct ttaaagtgtc gccattggaa ggaaccaatg aaatcaccgg
    15841 tgccgaagat gaagcgacca cagcttataa cgagcaatta gggtttgatg gttatggcaa
    15901 gtttgaaaat cttccttatg gggattattt acttgaagaa gtagaagccc cagaaggttt
    15961 tcaaaagatt acgccattag aaatccgttc tacttttaaa gaaaataaag aggattatac
    16021 caagagtgat tatatcttta ctattaccga aaaagggcaa aagcaaccga ttaaaacggt
    16081 aacggtccct tatgggaatc taaccaatga gaatttctca gtcagtttga accgtttgat
    16141 gctttatgat ttgcctgagg aagaagatag cttaacttct cttgcgactt ggaaagaagg
    16201 agataaagaa ttgactactc ttgattctac cgagctagtc gataaattga gctataatct
    16261 acatgaaatc aaagaagact ggtatatcgt agctcaagcc atcgatgtag atgctacaaa
    16321 agctgcccaa gaaaaagata aaaaagccaa accggtggcg attgcggaaa caaccgcaac
    16381 tttagcgaat aaagagaaaa caggaacttg ggagattctg cataaattaa ctgtcgaaca
    16441 agttttagac aagaccattg tcttattcaa ctatgtgtat gaaagcaaag aagcctatga
    16501 agctggtgat gagccggtag cgaaggatgc tagcttgaac aatcaagcgc aaactgttaa
    16561 ttgtatggtg gaacgtcatg tttccatcca aaccaaagcg cacttagaag aaggttctca
    16621 gacctttact catggtgacg tggtagatat gtttgatgat gtatccatta cccatgatgt
    16681 actagatggc tcaaaagaag cttttgaaac aattctgtac gctttatttc cagatggtac
    16741 aagtaaagaa atttggaaat ctggcaagat tgattacgaa gtgaatgaca aagaatttac
    16801 caaaaccgta cttgcggaaa aagtggatac aagtaaatat ccagaaggta caagttttac
    16861 cttcaaagaa attaactatg acaaagatgg gaatatcaat ggaaaacaca atgaagattt
    16921 gaaagaaaaa tctcaaactt taacgcccaa aaaagttccg acaacaccaa gtacaccaga
    16981 acaatctgaa atgccaactg ttccaagtgg ctctcaagaa tctagtccta cagtgaaggc
    17041 attccctcaa accggggaga aaacttccaa tgtgctactg ttcattggct ttagcttgat
    17101 ctttgcgacg gctggctatt atttttggaa tcgtcggaac taaggtgata agatgaaaca
    17161 tgtaaatagt agaaaacaat acgcgcgacc gcctcctaaa aaagtggtcg cgctattttt
    17221 gtattcaaaa ctaacaatga aaagaggaaa aaatatggaa ttgaaatttg ttgtaccaga
    17281 tatggtcgag acgttcggaa aactaacata cggtggtgaa ggtgaagttc tatcggaagg
    17341 gtatggtcga aatactacaa ttgttggtcg aagttaccat ctgttttcaa ataaacaacg
    17401 ggccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt ttgaccaaga
    17461 tcaaccacta aaagctgtaa atccccactt aattgccaaa ggctatgaaa ttgagaatcg
    17521 tgggtttacc gattacgtgt tgtatgttga tgatttagtg aaagcatagg aggaaaagaa
    17581 gatgagatta acagaaggta ttgtcgtaga ttcaggatta acctttggca agttgcgttt
    17641 ttccgcatta cgtcgagaag tacgcaagca aaatgaggac ggaacggtta gtaacgaggt
    17701 aaaagaacgg acctataatt tgaaatcctc cgcgcaaggt cgaatgatcc aagtcagtat
    17761 tccagcaaat gtacccttac gcgagtttgc ttatgatgca gaggtagagc tggtcaatcc
    17821 aatcgtggat acggtcgcca attatgtttt tcgggaagga accaccgtta attggtttat
    17881 caaggcagat gatttagtct tgaaacgaca actgaatcaa ggaaaaccta cgaaccaaaa
    17941 tgaaggaaag aaataggtga agaatatgga aattaatgtt gaacatattt tagattgtct
    18001 cgatcaattt ggcaaaggtg agctaacgga ggaacaactc actaacgcat taacctatga
    18061 tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg atgccaaaga
    18121 agactttgat gtactggatt ggttagggga agaagagtct ttctttatgg ttgtcgaggt
    18181 aaatgaaagt ttatgtcggc aggcagaatc tgttttggaa gaaatcgatg tggagatgcc
    18241 ggatgccatc gaaagatttt taaaacaatt agtggaaacg aaacaacttc ccgtagtagt
    18301 aaatgactag cataactttt tgaaattgcc caatgaaatc ctattgggct ttttcgactg
    18361 gaggtggaaa ccatcaaaat gtacaaagga catcgcatac gcgcaggaga tcaacacttg
    18421 atttatcact ttgttcttgg ctggctaatc tttttgttca ttggttggat gagcgtcttt
    18481 tatttacaag aacttagaca atttgatatt tctaaggtat cattttccac tagtgaaatt
    18541 ggctggtcta tgaaggatgt aatctgcttg ctaggaagtc tcgctctttc aggaagcatg
    18601 atgttacttt atatacactt ttttcaggat cattggcgaa gtctttggca tcgacaaaag
    18661 ttagctcgga tgattctaga aaaccattgg tatgaagtaa agcagactca aggtgaaggt
    18721 tttttcaaag atttgaatag tagttggacc aaagagagta tcagttactt ccccaaaatt
    18781 tattatcgaa tgaaagatgg cttactttct attcgcgttc aaatttcact gggaaaatat
    18841 caggatcagc tattgaagtt ggaaaataag ttagaaagcg gactgtattg tgagttagta
    18901 gaaaaagaac tcaaagactc ctatgtggaa tacaccttgt tatacgatat gattgccaat
    18961 cgaattggga tagaagaaat agtggcagaa aatggggcgt tgcgattgat gaaaaatcaa
    19021 gtatgggcgt atgattcctt gccgcatatg ttaattgctg gcggcacagg tggtgggaag
    19081 acctatttct tactcaccat tattcaagca ctattgaagt cagatgcaga attgtttatc
    19141 ctcgatccca aaaatgcgga cctcgccgat ttaggtacga taatgcctca cgtatactcc
    19201 caaaaagaag aaatttctga atgtgtggaa gatttttacg aacgcatgat agctcgtagt
    19261 aggtcaatga aagaaatgcc caactacaaa acaggagaga attatgcgta tctcggactt
    19321 ccgcccaact ttttaatctt tgatgaatac gtggcttata tgggtgcgaa tcgttttcct
    19381 acaagcatcg aatagatgtg aaatggaagc ctattgagat tatcaatagt gacaactaat
    19441 gttttgagag atggaatgaa agccttcaag ttgagctgaa acatccccgc taatactcct
    19501 acatgctcta atcaatgaag tagattggtt ttggtatgaa gctaggtgaa gtcgtctgaa
    19561 atctgcctaa gtgcttgagc ataaggctga ctatagggcg ggcggctgaa aaagataact
    19621 atggtaagaa tgtctgaaaa tgactgacga atctgcgaat gtacgggtct aaaaggctga
    19681 tcttattgaa agataggaac atacggacgt atggtgagat accgacaagt aaggttccat
    19741 gatatgaaaa tcgggatagc taacgaagaa gctataattt cacaggctca tagtagatac
    19801 ctaaggttat acatgatagc taaaaacatt ggaacgatgt aagcagaaaa ctatcaatta
    19861 aatgactact tctagatagg tgcatataag taataacgaa attgctttgt ttctgtgaaa
    19921 gtaagggcac agtaccgatg aagcgtctaa caaatgtgga gggatagccc taagtctatt
    19981 taataacaat gaatgttgaa acagatgaag ttcaaggatc tggtaagaac gtgggaaaat
    20041 ccgttatgga ggtgccacgc ttgaacacaa aaatacgtta ctgggaatat tatgatctac
    20101 aagaaacatt cgatcagcta tttactcaaa gtagaaacgg gaaaaagttt tatcaattat
    20161 atgagttaat catttctgaa aataatattc ttttagcata ccgtactatc aaggcaaaca
    20221 aggggtcttc gacaccagga acggattcct tcacaattga taactataag gaaatgaacc
    20281 aagcagagtt tatccatctt attctcagtc atttagaaaa ttacaaacca aaatccataa
    20341 agcgagtaat gatacctaaa cccaatggtg agaaacgccc attaggaatt ccatgcatga
    20401 ttgatcgaat gatccaacag atgttcaagc agatactgga gccaatatgt gaagctaagt
    20461 tttatgaaca tagctacggt ttcagaccat tgagaagtgc aaagcatgct ctaggtcgca
    20521 ttatgtatct cataaacatc agtaaaatgc actatgcagt agacattgat atcaaaggtt
    20581 tctttgataa tgtaaaccac cgtttactga taaaacaatt atggaatata ggaatttgtg
    20641 acaaacaagt cttagctatt ctttcaaaat ctctgaaatc acccattcaa ggagaaggta
    20701 tcccgagcaa aggaacgatc caaggaggta tcatttctcc actattatct aatgttgttc
    20761 taaacgactt agaccattgg gtctcaaagc agtggcatac ctttgaaaca aagtatccct
    20821 atactaaagg ttacaataaa tttcgtgctc taagagatac aaatctaaag caaggctata
    20881 ttgtcaggta cgcagatgac tttaaaatca tgactaatga ttatccgaca gcattaaaat
    20941 ggttccacgc tgtcaagctt tacctaaaag acagacttaa attagatatt tccaatgaga
    21001 agtctaaaat cgtcaatttg cgtaaatgta agtcggaatt tttaggattc gctatttgcg
    21061 tgaaacaaaa agggaaaaaa tgggtttgta attcacgtat ttctaacaaa aaaaaggatc
    21121 agattaaaga agagattaaa caaagaatca aagatattca aaagagtcct acagcacaaa
    21181 atgctctcct gtttaatgct ctcgtgctcg gtgttcataa ttatttcagg tatgccactc
    21241 atattaattt agatttgaat aggatgaact acgttctttc attcactact tacaaccgat
    21301 tatcaaattg tagtacaaga tgttacccca acaaagcttc cccaacttac aaaaagtttt
    21361 actcaacaac gatgaaaacg tataaagtag ctggagtcta tttgtaccct ctttgcgacg
    21421 tatctacaaa aacaatttac ggatttaata cggaggatac accgtttact ccgtttggtc
    21481 gtcaaagatt ggagcacaaa agcctacagt ctcttgtgtt ccaagaatta agaaaattga
    21541 tggaatcgaa aataccgaat agaacggttg agtatttaga tagtagaata tctcgttact
    21601 caatgaaatc aggcaagtgt gagattacaa aacaattttt gccagcaaaa gccgttcact
    21661 gtcatcacta tttgccaaaa agtcttggtg gagacgacaa attcgataac ttgagaatta
    21721 ttcataagga tatccatctt ctgatccata caacaaataa aatgataatc gatcactacg
    21781 tgcatgaact gaaactggtg ccagagcaaa tagcaaaaat caacctttat cgaaagatgt
    21841 gtaatctgca aaacattcaa tagaaaataa atagatggaa cgccgtatga ggtgaaagtc
    21901 tcacgtacgg tgtggagcgg gggaaaagat ggagattata tcaaattctt acctatcgct
    21961 atagatgtta acgacaaaag aaagtgcggt gattttgaat aagcttaaac aaatcgtgat
    22021 gttaggccgt caatctggtt tctttctgat tctagcttgt caaaggccgg atgcgaaata
    22081 tctaggagat gggattcgcg atcagtttaa cttccgtgtg gctttgggtc gtatgagtga
    22141 gcttgggtat tccatgatgt ttggagaagt cgataaaaat ttttttatga aacgcatcaa
    22201 aggacgaggt tatgtagata cagggggcag tgtgattagt gagttttata cgccacttgt
    22261 accaaaaggg tatgatttct taagggaaat tagcaacatt gttggattaa ccgttcatac
    22321 ggaaagagaa aataatagtg tgtagcctag gaatatcaat aatcctgggc ttttttattg
    22381 aggtgattta tgacattttt agatttattc gccgggattg gtggctttcg cttaggtatg
    22441 gaacaagccg gtcaccagtg tatcggtttt tgtgaaattg atgaatttgc tcggaggagt
    22501 tataaagcaa tccatgatac aaggaaggag gtggaaatgc atgacatcac aagtgtatca
    22561 gacgaatttg ttcgatccct cggaccagtg gacatccttt gcggcggctt tccgtgccaa
    22621 gctttttcaa ttgcgggaaa gcggcaagga ttttccgata ctcgaggtac tttattcttt
    22681 gaaatcgctc ggttcgccac tcttctccaa cctaagtttc tattccttga gaacgtccgg
    22741 ggattgctca atcacgaagg aggggctacg ttcgagacga tcctccgaac gttggatgga
    22801 ttggggtatg atgtggaatg gcaagtgctt aactcaaagg cctacgttcc ccaaagccgt
    22861 gagcggatct tccttatcgg acattctcga gatgcatgta ccgaacaagt atttcctatc
    22921 attggatcgt cttcaacatc tgatcaaaac atcagaaact tggtaaatat caatccttcc
    22981 aatcgaggaa tgggtggcca agtctatggt tcagatagcg tagcaccaac cttgaccggt
    23041 gatgaaggaa ttaaaattgc cttaccggta aaggatggga tttctgtagc gggtatgttg
    23101 tcgggaaatt ttgaacaagg caataggatt tatgaggtaa ccggaacagc ccccactttg
    23161 tcaacgaaac aagggggaac gaagattatg attcggaaaa atgaaacaga atatcaagag
    23221 gtgaagccag gggacagtgt gaacctggct tttcctaatt ccacaagtcg ccgaggtcgt
    23281 ctgggaaaac aaagtgtgca taccttacta acaggagacc aacaagcggt agtcacggat
    23341 cagtaccaga ttcgaaagtt aacacctagg gagtgctggc ggcttcaggg ctttccagat
    23401 tgggcttttg atcgagcttc tcaagtcaat tcagatagtc agttgtataa acaggccgga
    23461 aactcagtaa cagttccggt catttttgat attgctagaa gattaaaggg ggtaaaagca
    23521 tatgatttat gacgagttga ttggagaaat ctactggatg attggcaaga ttcaggcaga
    23581 tccgaaattg gaagaagaac tgtaccggct caattttgat attcgcaaaa acggggtgaa
    23641 agtccaggtg attcgtatgt agtggatgag gaaacggatg ctcgaatgga gctcaatcaa
    23701 gtcatcggtg aattggaacg aattgcggac cttgccaaag aacctgatat ccgccaatac
    23761 ttgtttgaga taaaagcaga gttggaaatt gagggtatca ccgcggagta atagggaaat
    23821 aacccactga taggcactga ccgcggggga tttgcgaagc agaaatcctc cgtggtgctg
    23881 tgcctgttca tggacggcgg cagccggcca tgatgtctca ccccccgtat ctaacagggg
    23941 ggtacaaaaa cacaaaaaac aagcaagtaa ccataaaaaa tgcttgttgt atcaaggata
    24001 ttaagtaatt tgtataggtg ttaactcatt tggaaaagtt aacaccttga atttggaggt
    24061 gaaaacttgg cgcaacgaaa tttagattat cggttattaa aagaacgacg caacgaatat
    24121 ggcgtttcac aaaataaatt agctactgct tgcggactta gccgcccata cctaaatcag
    24181 atagaaaacg gtggcgtaat cgcctctaca aagaccatga gaaaaatttt tgatcaactg
    24241 gaaagtttca atcccgattt acctttaacg ttactatttg actatgtaag gattcgcttt
    24301 cccacaacgg atgtgcggaa aattattcaa gaaattctcc atttgaaatt cgagtatatg
    24361 cttcatgaag attacgcctt ttactcctat caagaacaat atgtcatggg cgatattgta
    24421 gtgatgttat ctcatgaaga agataaaggt gtccttttgg aattaaaagg tcgtggttgc
    24481 cggcagtttg aaactttttt actcgcccaa aagcgtagct ggtacgactt ttttgaagat
    24541 tgtctaaaag ccggtggcgt gatgaaacgc ttggatttag caatcaatga tcgagtgggg
    24601 ctactggata ttcctgattt aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt
    24661 cgtaccttca aaagttaccg ttccggggaa ctgttgaaag ctgatgaaaa ggatggtatg
    24721 ggcaatacct tatatattgg cagcctcaaa agcgaggttt atttttgttt gtacgaaaaa
    24781 gattatgaac aatatatcaa gttagggatt ccattggacc agaccgaaac gaaaaatcgt
    24841 tttgagattc ggttgaaaaa tgaccgtgct tattatgcga ttcaagattt gttaaaaggt
    24901 cgtagcattg aaagtaccac gttttccatt atcaatcggt atttgcgatt tgcggataaa
    24961 gtagatggaa aacggcgaac caattggccc ttgaatgaac aatggggtcg ttttattgga
    25021 cggaatcgga aagagattca attaacttct gaaccgaaac cgtataccat tgaacgaaca
    25081 ttaaattggc taggtagaca agtcgcccct acgtggaaaa tggctaagga gttggatcga
    25141 ttgaatcaaa caacgtatat ccaggatatg gtacagaatg cgcgactctc ggaccggcat
    25201 aaaaagattt tggagcaaca aagtatggca atcgaaaact tgattgtatg aaaagaggaa
    25261 ttttgatgga aattgtaaaa gatatgttat tggtacttgg tggcagtatg cttggtgtta
    25321 ccgtgatgtg cttgatgaat gcaagtgcag cagcggattg agcaatggaa aagaaaggaa
    25381 agtgacaaaa tgaatttcgg acaaaattt
//
LOCUS       DAYYAR010000025        25264 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000025 DAYYAR010000000
VERSION     DAYYAR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.025"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..331)
                     /locus_tag="ACHAT0_000926"
     CDS             complement(77..331)
                     /locus_tag="ACHAT0_000926"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8450886.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(735..1517)
                     /locus_tag="ACHAT0_000927"
     CDS             complement(735..1517)
                     /locus_tag="ACHAT0_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450887.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1582..2418)
                     /locus_tag="ACHAT0_000928"
     CDS             complement(1582..2418)
                     /locus_tag="ACHAT0_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8450888.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2436..3845)
                     /locus_tag="ACHAT0_000929"
     CDS             complement(2436..3845)
                     /locus_tag="ACHAT0_000929"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8450889.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3859..5682)
                     /locus_tag="ACHAT0_000930"
     CDS             complement(3859..5682)
                     /locus_tag="ACHAT0_000930"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8450890.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6525..6851)
                     /locus_tag="ACHAT0_000931"
     CDS             complement(6525..6851)
                     /locus_tag="ACHAT0_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450891.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6897..7259)
                     /locus_tag="ACHAT0_000932"
     CDS             complement(6897..7259)
                     /locus_tag="ACHAT0_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8450892.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7296..7510)
                     /locus_tag="ACHAT0_000933"
                     /pseudo
     CDS             complement(7296..7510)
                     /locus_tag="ACHAT0_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7638..7973
                     /locus_tag="ACHAT0_000934"
     CDS             7638..7973
                     /locus_tag="ACHAT0_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8450893.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(8049..10046)
                     /locus_tag="ACHAT0_000935"
     CDS             complement(8049..10046)
                     /locus_tag="ACHAT0_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450894.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(10144..10419)
                     /locus_tag="ACHAT0_000936"
     CDS             complement(10144..10419)
                     /locus_tag="ACHAT0_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8450895.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10651..11385
                     /locus_tag="ACHAT0_000937"
     CDS             10651..11385
                     /locus_tag="ACHAT0_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8450896.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11424..12566
                     /locus_tag="ACHAT0_000938"
     CDS             11424..12566
                     /locus_tag="ACHAT0_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8450897.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12661..14052)
                     /gene="sufB"
                     /locus_tag="ACHAT0_000939"
     CDS             complement(12661..14052)
                     /gene="sufB"
                     /locus_tag="ACHAT0_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8450898.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14057..14527)
                     /gene="sufU"
                     /locus_tag="ACHAT0_000940"
     CDS             complement(14057..14527)
                     /gene="sufU"
                     /locus_tag="ACHAT0_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8450899.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14514..15749)
                     /locus_tag="ACHAT0_000941"
     CDS             complement(14514..15749)
                     /locus_tag="ACHAT0_000941"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8450900.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15746..17032)
                     /gene="sufD"
                     /locus_tag="ACHAT0_000942"
     CDS             complement(15746..17032)
                     /gene="sufD"
                     /locus_tag="ACHAT0_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8450901.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(17045..17818)
                     /gene="sufC"
                     /locus_tag="ACHAT0_000943"
     CDS             complement(17045..17818)
                     /gene="sufC"
                     /locus_tag="ACHAT0_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8450902.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(18015..18863)
                     /locus_tag="ACHAT0_000944"
     CDS             complement(18015..18863)
                     /locus_tag="ACHAT0_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8450903.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18887..19573)
                     /locus_tag="ACHAT0_000945"
     CDS             complement(18887..19573)
                     /locus_tag="ACHAT0_000945"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8450904.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19566..20600)
                     /locus_tag="ACHAT0_000946"
     CDS             complement(19566..20600)
                     /locus_tag="ACHAT0_000946"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8450905.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20663..20919)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20995..21333)
                     /locus_tag="ACHAT0_000947"
     CDS             complement(20995..21333)
                     /locus_tag="ACHAT0_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8450906.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21335..21691)
                     /locus_tag="ACHAT0_000948"
     CDS             complement(21335..21691)
                     /locus_tag="ACHAT0_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8450907.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21697..22884)
                     /locus_tag="ACHAT0_000949"
     CDS             complement(21697..22884)
                     /locus_tag="ACHAT0_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8450908.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(23075..23920)
                     /locus_tag="ACHAT0_000950"
     CDS             complement(23075..23920)
                     /locus_tag="ACHAT0_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8450909.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            24154..25119
                     /locus_tag="ACHAT0_000951"
     CDS             24154..25119
                     /locus_tag="ACHAT0_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8450910.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7388 a         5309 c         4282 g         8285 t
ORIGIN      
        1 gggatttcta ataaccgaca taacaaagaa acagtgtaat ccttacacca tttttcaata
       61 aattgaacaa gcgaatttag gttttccgag agaatatggc tgcggctttt tttaagattt
      121 caagttcctc atttaaacga gcattttctt ttctcagttc agctacttct tttccagtca
      181 gtcctgtgga ctgatcaggt aaatataaat tcttccattt gtaaatcgtt gcttcagata
      241 agccatattc tttcgctaac cctcgaacag aacgtccagt ttgattcagt tcaacaatca
      301 tttgtttaaa attttcttca tatcgggtca taaaaattca tcctctcgta gataagttta
      361 atttattctt gtctctacga aaagctgtct aagatttgat actaacacca agttatcttt
      421 agaaccattt cagatacata tgaaacaatt acatatgatt ttttcataaa aaaacagtac
      481 tgtctagtag tataaaatat acaaatttct taaaaatttt ttcactaaaa tcactacctg
      541 aaacagtcta aaacacttat atatcaacgt ttaatgaaac tatctccgtt tcatttcaga
      601 tacaaataaa tatagtcggg ctccaaatcc gaaaaaaaat acccccagta gcttatccta
      661 agctactgga gatgtataag tacggtcata taacaacggg gtagggtaaa taaatgaaaa
      721 agatggaaga atcattattc aataactttt ttaaatactt cgttgtaatc agttacctgt
      781 ccgttcttaa ataccacaga ataatctttt ttcccatttt caagtaaccg aagtggaatg
      841 tctttaccaa cttgctctgt cactgcatta ctgatacctg ttagattagc taataaatct
      901 gataaagatt gagcatctgt agaaatctct gtatcagtat ttatcgtgat tcctttatct
      961 tcatttacaa gcttccaagt aggtggtata gctgtgtatg tttcgatgaa tgaatcaatt
     1021 ctttcatcta tcgtcttccc agtatctact tctgtctcat tactcttaga tgaatcgatt
     1081 ctttcatcca tcgtctgctc cgtaactgct tctgtctcat tattctcacc ggaaactctc
     1141 ttcacaccca aacttatata ttgtccatct ttagtgtgca tagttaaaat tcccacgctt
     1201 ttactaataa attcactata accagttctt tcaatagaga cttcaatgta tttcacacta
     1261 tcatactcaa aagaaaaata gacatgtaaa agcattttgt tgtttacatc tacataataa
     1321 tcctttgact ctacttgaga ccttcctata cggaatacag ctttactgtc tgatatctct
     1381 ataccagcat ccttattatt aatctgccaa tctccaagta gctcttctgg caagctagta
     1441 gtttcttgtg aacactttac cacttcactg gtagaggtcg aaacattcac ctccgcactg
     1501 ttagaagaat tttccaagga atcaggagcc ttagcacaac caacaagtct ttatatattg
     1561 tgcagccgat attttcttca ttcagtacac ccttgaaata tgcagtgtta gataaagcag
     1621 ttcgtcctga tttgctgagg cacccatctc ttcttgaacg tactccttga ttttcaaggc
     1681 cgcccgatac tctttcggaa aacgctgctt cactgaagcc agcaaaaaat catccttctc
     1741 cggcaactct tccttcttaa tcattcgggc aataaaaaac tgtaaatgcg tcacaaatct
     1801 taagtaaagc atcgaatctt tatcaacctt tttttcggga aacagatatc ctagcatagc
     1861 caagatatcc tttagtgctc ttaccgagag catggtttct tccatatttg cttgttcact
     1921 ttgggcattt acaatgtgaa acgcaatgtt tccagcctcc tcttctggca actgaacatt
     1981 catttttttg ttgatcaatt ggatagcata gcagccgatc tcaaattctt ttgggtaata
     2041 cttttgaatt tcaatcagca ggcgattttg aaaaacaatg cctttttgac tgcgttcaat
     2101 ggcatgcttc aagtgatcaa ccaaggtaaa aaatagactt ttattcagct taaagtcaaa
     2161 cttggcgttg gcataatcga taattcgctt gacgcaaata attagatcat ccggagcagc
     2221 ctcaaacagt ttaatgtaat ccttttttag agcatcagaa agtgaatcta atttaaatac
     2281 tttttggatt ttgtcagccg gaaccaaatc gcctttcttt agttgaaatc caatcccttt
     2341 ccccatcaaa attacttctt tgccttgctt atctaagcaa agaattaaac tgttgtttaa
     2401 cgttttaatt actctcatta gattcaccgt tctctctaag ctaagccatt tcttgcgatt
     2461 actttttgat accaaaagaa gctttctttt tttattcgtt ttaattcttt aagattctca
     2521 tcggttcggt caacatatat aaaaccataa cgcttattga agccttgatg ggtactaaca
     2581 acatcaatcg ctgaccaagg taaataacca aaaacatcta caccatctgt taaagctgct
     2641 tgaatagcca gaatatgttt ggctagataa tcaattcgat cctggtcatt aattcgtcta
     2701 tcttcctcaa ctatgtcggg tgccccatag ccattttcag taattaaaat aggtaaatca
     2761 tagcgatcgt acaatttcct caatgttaat cgtagtccag tagggtcaac tacccagcca
     2821 tatggcgttt tatctacata aggattttca gctgcccggt agacaccttc ttctcccaac
     2881 atgatctgtt gatcccccgc tctagcggaa acatctgatc cgtcattttt gctggcagcg
     2941 atggtggcag ttgaataata attaatcgca ataaaatcgg gtttaccgga ggataaaatt
     3001 gcctgatcct ctgcctcaat tttaggatat aatccacgat cattcaaata gttttcaaac
     3061 aaataattgt attttccccg aactgctaca tctaagaaag accagcctct caatgtttcc
     3121 caattatggg ctgcaattgc atcttctgga ctggaggtag cttgatacat agccgttaaa
     3181 ttgattgctg gaccgatttt tcctttaaga ttcaactgat gatacaagtg aattactcta
     3241 gcttgagcta ataaaacatg gtgattaatt tgaaagagtt ctttttttga aggcagctct
     3301 ccacttggtg gcaaaccaat cgctccagga tgaaggatca tcgtgttttg ctcattaatt
     3361 gtcagccaat agttcacctt gtcaccatac tcagtaagca atacctgtac atagtccaca
     3421 aaataatcga tagttgctcg atttaaccaa ccgccctgct cctctagaaa ataaggtaaa
     3481 tcgaaatggt acatggtgac aatcggttcg attccctgat tcaggcattc atcaattact
     3541 tgatgataaa aagaaattcc cttttgattg gttttattat tttcaatatc cataattcgc
     3601 gtccaagcga tggaaaagcg aaaagctttt aagcctaatt ctttaaacag ctgtatatct
     3661 tctttatagt gatggtaaaa atccgaggca acttcaaaat tagctacccc ttctgtatgc
     3721 tgatacttgt caataataga cggttgtttc ccatcctcct gataggcacc ctctgcctgg
     3781 taggcactaa tggaggcacc ccataaaaac ttctctggaa atgctgaaag ctgtttgtat
     3841 tgcacaaaaa tcctctcctt aactaatttg catcaatggt tcgtgttcct ttaagcttcc
     3901 gcttgcttca ctaattcctg cgtaattttc actgttggtc actaccacaa tcactgtatc
     3961 atcgtagcca gcgtttctaa tggcagcgaa atcggcgttg attaataatt gtcctgctga
     4021 cacctgatct cctgctttta caaatacttc aaagggttcc ccttttaaat tcacggtgtc
     4081 gatccccaca tggatcaata attcaatacc ttctttagaa cgtagaccaa tcgcatgttt
     4141 agattctaaa atagtaatga cttctccctc aacaggtgaa taaatgctgt tttcttctgg
     4201 atgaatggct acgccctttc ccaatagtcc cttagaaaag acagcatcgt tcacttcaga
     4261 aataggacac acggttcctt ttaccggatt ttcaacaatg actgcttcgg ctattgccgt
     4321 ttgctctgat tcatcagaag tatcgtcaaa tccaattaga taagcaatga taaaggttgc
     4381 aacaaaggcg attacacacg taataattgc gtgaacaata ttcattggat tttctccaat
     4441 aaaggctggt aaggctgcta agccagggga aacaaaggca tatctgacta aaccgctcaa
     4501 gccagcatac aaaccagcaa ttgcaccacc aatcatggaa gcaactaatg gttttttcag
     4561 cttcaaggtt actccataga gtgaaggctc agtaattccc attaaagcag tgatcccagt
     4621 ggacacagct agctgcttca actctttgtt tttagttttc gcggctacag caaaggtagc
     4681 acctgattga gcaatattag aagccagcat tcctggccca ttgatcattt cagaacccgt
     4741 actgctcaat tgcatagtag cgattggcgt cattgcccaa gctgtacccg taaccaccaa
     4801 aaatggctgc aaaccaccca tcaaaaatgg aattaaccaa gctgctttgc cgttaatcca
     4861 ttcagccccc attgctataa gatcgttcag caacgttcca aacggtccaa tgacaaccaa
     4921 cgctaaaggc cccataatta aaaataccag catcggctta gtaaagaatt taataaacga
     4981 aggcgaaatt cgatccgcaa agggctctac ataggacatg atccaaacag taaagataat
     5041 cggtaaaact gatcccgcat aatctgcagc taataccgga attcctagaa aagaaacggc
     5101 atcgccagta gaaaaaagct gtgaaatatt tggatgaagc aaaatgccag caacagtaat
     5161 cgccaaaatt gggttgacat caaactttat ggctgcccca taggctaata gtactggcat
     5221 aaaataaaag gctgcatcag caatagtatt caaaatgatg taagtttgac tactgtctga
     5281 tagtacacca gtcaaagtca agacggccaa aatggctttt accatcccgg cacctgttat
     5341 agctggaatc atcggtgtaa aggtggtaga aatgacacta atcaatcttg aaataatact
     5401 ttcttgattg tctgaagagg ggcccttttt aattgaagtt cccacttcct tttgaatttc
     5461 cttgaaggct gtttggactt cattgccaat caccacttgg tattgtccac cgctatccac
     5521 cacactaatt acaccaagtg ttttttctaa ggctttcgta tcggctttcc ttttatcttt
     5581 gagttcaaaa cgcagtctgg tggcacaatg ggttaagctg accacatttt cttttccccc
     5641 aacctgttgc aatacatttc ttcctacttc tctgtaatcc attattctct ctccttaaag
     5701 cagtaaaaaa gaccaagtca aaccacgccc caatcaacat tgaagtgtta gtttgtcttg
     5761 gtcttgcctg cataatcagt aacatgccaa taacgttatt ttgctgtaat ccaataatag
     5821 cacattatga tagcgcaatc aacagggaat ttaatttttt atcttttcgt ttgaccgtat
     5881 agggtaatat aacagttaga tcacatcaac tagggtatat ctgcgtatac ttactgctaa
     5941 aataagtata tctaatatta actataccag taaaaccatt cctacataac cagcgggaaa
     6001 ttcccaaaat cgtgaaagca tcaaaaatgc atataacgga tcacacaact tcaacaattc
     6061 tgctcttggg aaaaattgat gaatttggtg agtaaaggat ggaataaaca gcattccaac
     6121 aatataggca acaatagtga tacttgtttt tttggtaaaa atcagtaagc aatagccaat
     6181 ttcaatcatt ccgagatata aaggtatcga tagaatgatt tgggctagta ttcttaaaaa
     6241 ttaactagca aatgtttaaa ctgatttatt tctaatctac atgtagtaag tatttcaatt
     6301 ccaccaatta tcaataaaaa taccaaacat atccataata attttttctt tttcatttct
     6361 tttatattgt tataacagtg aataacataa aaattcccga caatcattgt aatatttttt
     6421 tgtcacctgt attagtattt aatttatagg aaactataaa aactccctag tgaatttaca
     6481 cctttgggag ttcctgtttg gacggtatgc ataatacatt taaatcaatt taacattaac
     6541 acctcagcat atggtatctc cacatatata cggtctttct ttggatagta ctcaatattt
     6601 atgttgatag cacttccatt gtctaatata gctttagccc ggtatgtgtt atctccattt
     6661 tcatgagtaa tcgtcacatc atcacatgat cgatttaagt tgtactgttc ttgtaaaatc
     6721 tgtgttacca atggtttcga tgacttctct aatccagatg tacctgtcat ttttgaaaaa
     6781 atgataccta taatagccaa gacaacgata actataatga taaacttctt gttcatattt
     6841 actttattca actttttatc tcctatctcc aaaataattt gctcttatcc ctcttatcag
     6901 tacattgaca tccctaaaag tacaccaaag attgctccag ctacccctgc tatcatcaaa
     6961 atcgtaccca tttgttcatc tttatctcga tataagtagc ccataataac agcaacagca
     7021 ccaaatacaa caggaataaa tgctaacgct actaccatag aaatccaacc aataatcaaa
     7081 tacagcttcc ctgtctcttg tccatgtacg atttgttcct tcatttcatt aattgtctgc
     7141 tcagtacttt tctttactgc gtctgctcca gttgtctcat ctgtttcttg tagtgctccg
     7201 caaaactggc aatacttact ttcatctgtt atttctttgc cacattttct acaatacatg
     7261 tcttctcctc ctaaaaacat tcaattgttt attagttaac tgtttacttt attaaataca
     7321 tcagaaaaca gctcattcat tggtacactt aatatctcag aaagcatctt catttgatag
     7381 ggtttaggaa tcgttctccc tgtttgccac gaacttacac attgctgtgt aacattcatt
     7441 tttttgctaa ctccgtttga gaaagtcctt tttcttttct gtatcggctg agattactta
     7501 cttttttcat gattctcccc tcctgtttta ttatatacaa caatatgatg tatttcaact
     7561 cataaaatat aaaaaaactt caaataccat tacaaataca acaacttgtt gtactatact
     7621 gagatggggg tgtattaatg aaacagaaac aatatgcaaa ggtcattaga gaaaagcgaa
     7681 aagctctcgg gtggacacaa aaagaattag ctgaaaaaat cttctctaca caacaggcag
     7741 tagcaagatg ggaaaattct gtaacagagc ctaacctcga tagcttaacc gctctttcta
     7801 gagccctagg aacccctgta agccattttc tagacaatgt tgtcgtagat tatgaggaag
     7861 agtttttggc tctctatcgt tctcttagca ctgaagatgc agttcgaaca attgactata
     7921 tgaagctatt aaaaaggcaa gaaaatgaac gcaatcagct attgaaagat tgaaaaataa
     7981 ataacccacc gaactttaac ggtgggtgta ttgagatgaa ataagattaa gagctatgga
     8041 taacctgtct agtttaatac aattgggtct tttctcttcc ttactctccg tccatcatct
     8101 cttgttacac ttgtaaattg ataaagagga actaattcaa ctcctgataa tatcaattct
     8161 tttattactt catctatccc tgctcctttt gctaggtaga ctaaaaaact actacttgct
     8221 ttcacattgt atagtctctt aatatcctta gaagctcgtt caagagtgaa tccttgttta
     8281 acaaagtagc ctctgttttc ttttaaagca ttttccacgc tgttattccc aacattacgt
     8341 agacgttcac aaacagtgtt agtaagttct ttctctgtca ttagatatgt tccttgccac
     8401 gataagccct gtaaaacttc atgtgcttca tctaaaatca attgtttttc cttgctaagt
     8461 tctgatcggg aattttgagg aaatactctt ttagcctctt tttcgttaaa acatatctta
     8521 acccattcat aagaaaaata ttgatatgta aaatgaaatt tttccagaag tggaaggata
     8581 tcttcattca atttttgaat cacagaagtt gcattaaaaa cgtctaatcg atagacattg
     8641 cttctcttat gattaaagtt tcctaacgct ttctcttcct ttaacctatt taggtattcg
     8701 agcatcttca aaggaacatc ttcatcatct aattttttta tcaatcctat aaaagtcata
     8761 agtgatagta acttattaca tttatctatg tcataagttt taccatagta aaaactcatt
     8821 tttttagcta attgttcccc cgacaaaata caagtcacac tttccttact atcatcaaaa
     8881 aaattaacat cagaaagcaa gacatcatag acacgtcgca tttcctctag ataattcctt
     8941 attgtcctga aaagtttgtc tttcattgat tcaagagtgt caaaaagtac ttttgattcc
     9001 tctttcaatt ttcgaaaatg tgtaggatac aagtctaggt tctctaatag ccaattaaat
     9061 gtttctaccc gactgtcttt ctttaaaatt agcatcacaa ctccaataaa attttcactg
     9121 atatcacaat tactggaatg acaataaaaa atggaatgcc cagttttact ggtaaaaata
     9181 ctagcagagg gattatgatc gttgtgaaac agacatctta agtttttact tgacttaagt
     9241 aatttattca tatcaatggt taacaatctt tcataaatct cattaaaaga catgtcggag
     9301 gaatcaaaca ggcagtcttt aaaccactgt ttcatttctt ctttgtcgtc attttttatc
     9361 aacttaacaa aatcggtttc attgactagt tcatactctc tccctgaatc actttgacaa
     9421 tttttagaac atgtcatggt aaaatcaatc ggaagctcag taatatctaa catgttgtct
     9481 tcattaatta ttatcactgc atctgactta tatcctccaa aaaacattct attcgaagca
     9541 tttacattgt catcacaccc ggggatgttc tttttcaagt attcgtaaac agcaaccatt
     9601 ttaccatagt cagtaattgc ttcttttaat agaaagacaa tcttgtattt ttctagattt
     9661 tcagagtacc gaatactttt ttgtatcgca aacgcagaat tatgaactaa attgatagat
     9721 aaaacctttt ctatgcttaa gtatctacta tcatctttac tcaaatctac taccttcccc
     9781 ttctcatctt taacagtgtt atcgacatcc agaataatta ctctggtttc cacaaaactc
     9841 tccttgtcat tgcctttatt ctttgtcttg cttggaataa atgattgtga tgaatttagt
     9901 atctctacaa actcatctct tgtaacttct ctatagttat ctaaacacct atctcttaca
     9961 tcagtttttt ctttttttgt aactggcttc tctaataatt tttctttatc tacataaatt
    10021 ggaatattaa tcattgatgt ttccatttgt gtttcctcct tataaaattc tgttaaagac
    10081 taaaacatac agataataaa aacaaactct ttattactat atgttttagt cttatttgtt
    10141 tactcaacct ttataccgtt ccatgaaact atcaatatca gattttttaa atctaatgac
    10201 cgtatcaatt tttataacag gtaacccgta ttgagaaaca tattttctaa tcgttcctac
    10261 tgatacacct agatattcac ttgcctgctg atagtccata tactctagag tgactccaga
    10321 aataatgtct tctagcatag gctttagcac tgtaattatc tctttcactt gttgttcaac
    10381 ttgtatagaa acaatgctat caatgatttg tagagacata tttccccctc ctttcgtgat
    10441 tgcttgaaca aattttctaa gaattatctt cgtatttttt gctcaaaata aaagtatttt
    10501 gttattaacc gattaatcaa acaaaaaatg attaccagtt catcgacata cacataatac
    10561 ctcgataaaa aagaggtgtc aatatctttg taaaatttat ttacaacacc cctttttcga
    10621 ggtatactaa ttttaggagg tgtggtcaat atgataaaac gtccaccaat aaattattta
    10681 gaacgaaaaa agatactcgg aacaaaaata aaagctataa gaaagagtaa aaaattaaca
    10741 caaccagcat ttggtcttat gattaacaac ggtcaattaa ttgacaaaaa aactatctac
    10801 gaatgggaga agggaaccta tttaccaata ccagagcgat tatcccgaat tgctgatttg
    10861 ggaaatatga gtatagagga gttagtttgt ggcaatgtag aagaatatat tttgggaatt
    10921 attctttata gagatagcat tgtactagat ggtataacat ttccagataa aaacctgttc
    10981 cagcatcttc gtcaacagtt cccaccagta cactctaatt tagatacatg gctggataga
    11041 tattccaaat tagaaccaga gatgcaagaa tttattgcta acaagacttg taataaagtt
    11101 aaaaatgaaa aaattagtct tttcaatatc ttaaaaatag aagaattatt catcaatgct
    11161 attgtagaag aatttgacaa taacattctt ttcctcacta gcagtataga agaactacta
    11221 gaaagaatgg tagatgaatg gctacctatt caattgaaag acatgtctta tcccgaagaa
    11281 gctgtgagag aaattactga taatataaac aagctagaac aaacgatttc atcaattggg
    11341 aaaaaataca ctaagaaaaa aatgaaggga ggtgatacca tctagtggta agtactcctt
    11401 gtaaactaag aggagatgat ttcatggcaa aaatatcaaa gtacaaaaag aaaaacggtc
    11461 aagtggcgtg gatgtataaa ggacacattg ccaccgatcc acgaactggt gaaaaaataa
    11521 atactacgag aagaggtttt agtagcaaac aagaagctca aaaagattat gacgagtaca
    11581 gacatcaaat attatacggt gttaaaaaga aagcacctga tatgacattt gaagacctgt
    11641 ataatgaatg gataactcat cagcgtacca gtgttaaagc ttcaacaatc gcaatctcag
    11701 taagatacgc aaataatcaa atacttccag catttggtaa gcttaaacta tcgaatatct
    11761 ctgtcccata ttgtcaaaaa gtggtagatg aatggcacag taaatatgaa agttacgatt
    11821 atatgagaaa acagactgca caaattttac gctacggggt tgcaatgcaa tatattgaca
    11881 ataatcctat ggaaaaaaca cttcttccac gaaaaaaaga gtatgagaaa aatcgtaaat
    11941 tttactcaaa agaggagctt aataatttac tggatgcttt taaagatttt ggtaacatga
    12001 aacaatacgc cttcttcaga ttattggcat acacaggtat gagaaagagt gaagtactcg
    12061 ctttacagtg gaaagacata gacacgttta ataaagaact acatgtcaat aaaactcttg
    12121 ctgtagatga atttggtaaa gttataattc aatctccgaa aacaagagct tctagacgag
    12181 ttatctcttt agatactgag actctttcta tcctcaacaa ctggaaatta cagcaaaaag
    12241 aagagtattt aaaacttgga tacaatacta gctctaaaga gcaacatgtt tttacaacag
    12301 ttaaaaacac tctatacatt ccaaacactg ttaatgattg gttaagatat attttgaaaa
    12361 aatataactt gcctcgtatt acacctcatg ggtttagaca tacacatgct agtttattat
    12421 tagaagcagg agaatctgtc aaggtagttc aacaacgatt agggcatgaa aatagtaaag
    12481 tgacattaga catctatgca catattacaa ataatgcacc taagaaaaca ggtcaagact
    12541 ttgctgatat gatggctcat cagtaaaaga gctggtcaaa atctggtcat ttctagtata
    12601 caggcaaaac aaaaaagcta gaacccttat atatcaaggg ttctagctcc tataacaggt
    12661 ttacccaaca ctgccttcca tctcataact gatcaatcga ttgagttcga ctgcatattc
    12721 cattggaagt tctttggtga atggttcaac aaagcccatg acgatcattt cagttgcttc
    12781 agattcggtc aggcctcggc tcatcagata gtagagttgt tcttctgaga tttttgagac
    12841 tttggcttcg tgttctaaag agacttggct gttgtggatc tcattgaatg ggatcgtatc
    12901 ggatttcgac cattcgtcca tgatgatcgt gtcacattcg atatgagaga tcgaacccgc
    12961 actgtttttg gcaaaggtta cttgtcctcg atagtttact tctccgccgt cttttgaaat
    13021 tgatttagag acgatagagc tagatgtatt tggtgcattg tggatcattt ttgcacccgt
    13081 atcttggatt tggtttgctc ctgcaaaggc gatggagagc atggtgcctc gtgcaccttt
    13141 tccatctaag taaacacttg ggtatttcat cgttgttttt gcacctaggt ttccgtcgat
    13201 ccattcaacg gttgcgcctt cgtatgcttt tgctcgtttg gttactaagt tgtacacatt
    13261 gtctgaccag ttttggatcg ttgtgtaacg gcaatacgca tcttttcttg taaagatttc
    13321 tacgatggcg gcatgcaggc tgttacttga ataagtaggt gccgtacaac cttcgacata
    13381 gtggatgctt gctccttcgt caacaatgat cagcgtacgt tcgaattgcc ccgtgttttc
    13441 cgcattgatc cggaagtacg tttgtaatgg aacatcgact ttgacgcctt ttggcacgta
    13501 aataaaggtc ccacctgacc atacggctga attcagcgct gctaatttgt tgtcggttgg
    13561 cggtacaagt tttgaaaagt actctttgaa taattcaggg tattctttca aagcagagtc
    13621 agtgtcagta aagacgattc ctaacttttc gaattcatct ttcatattat ggtacacgac
    13681 ttctgattca tattgtgcgg aagcaccggc tagataagct cgttcagctt ctggtatacc
    13741 aatcttttca aatgtatctt tgattttttc tggtacgtct tcccagtcac gagcgggttt
    13801 gtcacttggt ttttggtaat atttgatggc atcgaaatcg atgtctgaaa ggtcggggcc
    13861 ccatgtttgc attggcattt tgttaaatgt ttctagagat ttcagacgga attcaagcat
    13921 ccattctggt tcgcctttga ttcttgacat ctcacggatc acttcttctg tcaagccttc
    13981 tcctgtactg aaaaccggtt cgacatcatc gtgaaacccg aatttatatt cttctaattc
    14041 tggtacgccc atgttctcac tcctcttcct tgtggtaatg atggaaagct gcggtaccat
    14101 cgttttccat tgcttttcct agtgctttcc aggcaagtgt cgcacatttg atccgagcgg
    14161 gaaatttagc tacaccacca agcatagcag catcgcctaa ttggtcggga tctttgactt
    14221 cttttccttg gacaagctgt agaaagtcta cagttaaatg ttctgcttct tctattgatt
    14281 ttccgatgac ggcgtctgtc atcatcgagg cacttgccgt actgatggag cagccactgc
    14341 cgtcaaaggc gatatttttg atgattccgt cttttacatc gacttgcaat tcgatcacat
    14401 ctccacatgt ggggttgttc aattcgatct tgcttgtcga gtcagtcaag cttccacgat
    14461 gatgcggatg ggaagaatga tccaagatca cttgacggta taaattgtct aatttagata
    14521 gtcccatgtt ggaaaaactc ctttgttgcc atgattgctt caactaaacg gtcggcgtct
    14581 tgcgttgtgt tgtagaaata aaagctcgcg cgagcagtcg ctgatacttc aaggtacttc
    14641 aatagcggtt gcgcacaatg atgacctgct ctgacggcta ccccttccat gtctaaagct
    14701 gtcgcgacat catggggatg taacccatcc aaattgaatg caacgactcc tgtccgaaaa
    14761 ttcgggtctt gtggaccata aacagtcaat ccatcaattg cttgtaattt tggcaacaca
    14821 taagcggcta gttcttcttc atatcgatgg atattgtcca taccgagttt ttgcaaataa
    14881 tcgattgcat ggcctaatgc gatagcgccg gcgatatttg gtgtccctgc ttcgaatttc
    14941 caaggtagtt ctttccatgt actgtcgtat aattcgacaa aatcgatcat ttcgccacca
    15001 aattcaacag gttccatttc ttctaaccat tttcgtttac catataaaac accaatacct
    15061 gttggtccgc acattttgtg tccgctgaaa gcaaagaaat cacagtctag tgcttggaca
    15121 tcaatggcca tgtgcggggc agattgagca ccatctacta cgatgacccc gccgttttca
    15181 tggacaagtt cagctaattc ttgaacagga ttgaagacac ctaagacatt ggatacatga
    15241 gcaatggaga cgattttggt tttgtccgta atctttttcg ctgcatcctc catatccagg
    15301 aagccttctt ctgttagttc gatatatttc aagacagcac ctgttcgttt tgccaactgc
    15361 tgccacggga taacattaga atgatgttcc atgtaagaga gcactatctc gtctcctgct
    15421 ttgatatagc ggtcgccgaa ggttctggct acccaattca agcttgtcgt cgtaccacga
    15481 gtaaacagca cttctgctgt ttcttttgca tggatgaacg cccgtacttt ttctcttgct
    15541 tgttcataat catgggtcgc gcgttctgct aacgtatgga caccgcgatg aacattggcg
    15601 ttatcgtgtt cgtaatagta cgctaatgcc tggagtactt gttttggctt ttgggtcgtc
    15661 gctgcattat ccagataaac taaaggttca tcattgacgt cttgaaataa gataggaaaa
    15721 tcattgcgta gctttttagc atcgatcatc cattcaactt cccttcgatt acttcgacta
    15781 attctttccg aacttgttct acagggattg ctgttaagac agaaccgagg aatcctcgaa
    15841 tcactaagcg ctctgcttct tctttatgca atcctctact catcagatag tacatttctt
    15901 ctggatcaac tcgtcctaca ctggcagcat gtccagcagt tacttcgttt tcatcgatca
    15961 aaagaatagg attcgcatca ccacgtgctt tatcagaaag catcaagaca cgactttctt
    16021 gttgggcatc tgcacctttt gcccctttca agatgtggcc gataccatta aaggtcaatg
    16081 ttcctttttc tcggatcact ccgtgttgca aaatatgccc gatcgtatga ggggctttat
    16141 tggttactcg tgtatcgatt ccttggattt gtctgcccga gctgattgcc acgattttga
    16201 cttctgcgtg tgcaccttct cctgctaaat ctgagtcaaa gtcagcgatc acatgaccgt
    16261 cattcatgac accaattgcc cagtcgacag aagcatctcg taaaatgtgc ccacggcgat
    16321 tcatataaga agtaatattt tctcctaatt gatctacggc tgaatatttg atttttgaac
    16381 cagctttagc gatgacttcc acgatgatat tacctgatga ttttgctact tgttctttgg
    16441 ttgtctggaa acgttccaga taagaaaatt cactatgttc atctgccacg atcaacacat
    16501 gcttgaagaa gtgctcatcg gatgcgccat cttgaatgaa taaggactcg agtggttcat
    16561 cgatcacgac atttttaggc acatacagga aaataccgct gttcataaaa gctacatgga
    16621 gtgcagtcaa ttggtcttcg ttcatgttta ctgctttttt catataatat tcttggacta
    16681 gttctggata ttcgatcatt gcagtgaaca agtcggtaaa aatgactcct ttttctgcta
    16741 attccacagg cagttgttca aaaatcgtcc atgaaccttg ttggacaagc acaggattgt
    16801 ctttcattgc atcaaatgcc ggaatcgtgc ctgtttcact gatggtttgt gtttctttca
    16861 catcaaataa cgaccagcga tggaatttca cccgatcgat atgcggcaat ggcagttcat
    16921 ctgctttcgc tagtgcgtct tgacggaagg ttctcatcca atcaggttcg cctttactta
    16981 aagaaaaatc atttaccgcg tcaagataat cgagccagtt tttttctttc attcttgctc
    17041 ccccttacgc ttcttcttct ttatagtcga ttcctaattc tttactgata ccagcgtagc
    17101 cttctgcttc cagacgttta gctaggtcag cattccctgt catgactact cggccttcca
    17161 tcatgatgtg aacgacatca ggtgtgatat agttcaataa tcgttgatag tgggtgatga
    17221 tcaatgcacc gaaattgtct ccgcgcattt catttactcc tttagcaacg actttcaaag
    17281 catcgatatc cagacctgaa tcgatttcgt ctaaaattgc aaatgtaggt tctaacatca
    17341 acaattgcag gatctcattc cgtttctttt ctccgccaga aaaaccttcg ttcagataac
    17401 gttcagccat ttcttctggc atatttagta aagccatttt ttcatctaac tttttcaaaa
    17461 atgccataac agaaattttg tcgttttcat cacgtttcgc attgatcgct gcacgcatga
    17521 attccgcatt cgtgatacct ggtatctcac ttggatattg catagctaaa aataagccaa
    17581 gtcgcgcacg ttcatctact tccaagtcta aaacattttt cccgtcaaac aaaatttcgc
    17641 cttctgttac ttcatagttt ggattgccca tgatagcggc agataaggta gattttcctg
    17701 tgccgttagg tcccatgatg gcatggattt ctccggtttt cattgttagg tttacgcctt
    17761 taaggatttc tttttcctcg atcgacacat gtagattttt tatttctaag acagacatgc
    17821 attatacccc caaattctct ttcgttctca ctcattttag actaattgag aatgaagcgc
    17881 aatgaccttc cttactaaaa tcaaatatct tttatttatt attaaacaat tttgatttct
    17941 aaattagaat aggtataaaa aagatgagaa ataagccatt acagacttat ttctcatctt
    18001 ttttgttcca taccttattt tgcatcagcc tttactggtt taacagagcc gccccatttg
    18061 tctgtgatcc attcttggac ttctttgcgg tgaagcactt caatcagttt tttgattttg
    18121 tcactgtctt catcaccttt acgagtcgca ataatattca cgtaaggaga gttgtccttt
    18181 tcaagcaata cggcatcttc taatggattc aaccctgcgc cgtaagcgaa gtttgcgttg
    18241 attgcaacta ggtcaccttc atcattttgg tacgtagaag ctaaaagtga tgggtcgatc
    18301 gtatggttga acttcaagtt cttagggttt tcttcaatat catcgaatgt tgctgtttcc
    18361 agatctactc catctttgac tttgactaag ccagcatctt gtagaattgt gatgatgcgt
    18421 ccccagtctg attcagaatt tgaagtaatg attgttgcac catcttttaa ttcggaaaca
    18481 tctttgactt ttttagagta caagcccatt ggttcgatgt ggacagcacc ggcattgaca
    18541 aagtcatatc ctttttcttt gatttctttg ttcaaataag gaatatgctg gaagtaattg
    18601 gcatcaatgt cgccttcttc caaagattgg ttcggtaata catagtcatc gaatttgacg
    18661 atttcaagat cgattccttc atctttcaat aatggtttca catgttccaa aatctctgcg
    18721 tgcggtgtcg gtgaagcgcc gactttcaat gtcgtgtcgt ctgctttatt tgctgaatct
    18781 ttgctgtcgc tgttgttacc gcatgctgct aatcctacag tcagcaaaag cgctgctgca
    18841 aatccaaaaa ttttcttttt catatcctac atccccctat atcctattat cttttgtctg
    18901 ttttcttagt taagaaatca ccgattcctt gaacgataaa tacgattagt aaaataatta
    18961 aagtcgcaac gaatgtcact gttagattac cacgctggta gccttcctgc caagcaagac
    19021 ctccaagtcc acctgcgccg atcgcgccgg ccatcgcagt aaatccgatc atcgagatcg
    19081 ttgtaactgt taatccagaa atcaatgcgg gaatactttc aggtatcaag accttccaga
    19141 tgatttccca gtaacttgct cccattgcat ctgctgcttc cagtacacca gggctgactt
    19201 cacgaaaagc aatctctact agacgggcat aaaatggtgc tgcggataaa acaagagctg
    19261 gaatggctgc ttgcgcacct agcatagtgc ctacgattgc tttagtaaat ggaatgatca
    19321 aaaccatcag gatcataaat ggcgttgacc ggaaaatatt actgatgatc gatacgattc
    19381 cataaatgat tcgagcatac ggtttgtttt tgcgtccaat agagtaaagg atcaatccca
    19441 aaactaatcc gatgatcgtc actaagacca ttgagattaa tgtcatatat agtgtatcaa
    19501 ggatagcctg attcatcgtt tccgggttga tttgtttaaa atctagatat gtttctgaaa
    19561 agcttttatc cacgatggat cacctcaatt cctactcctt gttctttgat gaacacttct
    19621 gcttgatcta attctttggt ttcaccagtc atcaagacag tcaatgaacc aaaggaacct
    19681 tctttcactt gtttgatctt tccatacaca acgttgacat cgatgttata gcgtcgaatc
    19741 gcttgggaaa tgactggctc gttggcattt ctttcgctaa aggtcaaagt tgccaaacgg
    19801 ccgcttggat tttctttgat gagttcagct aatagttctg tcgaatcagc ttctggttcg
    19861 atgtcttgtt gtacaaagcg tttggttact tcttttttcg gttgtctgaa gacagtcaat
    19921 acgtctcctt cttctacgac tttccccaac tccatcacag cgactttgtt gcagattttg
    19981 cggatcacat tcatttcatg tgtgatcaag acgatggtca aattcaattt cttattgatc
    20041 gctaacaata agtctaatac ttcttctgtt gtttgcgggt ccaatgcgct agtagcctca
    20101 tcacaaagca ggatctcagg atcatttgcc aatgctcgtg cgataccgac gcgttgtttc
    20161 tgtccgccgg aaagttgtga aggatatgct tgtccgcgtc cttctaaccc gactaaacgc
    20221 aacagttctt ctgcccgttc tttacgtttg ctctttggta cgccagctaa ttccagcggc
    20281 agctggatat tttcaagtac agttcttgac cataacaggt tgaaatgctg gaagatcata
    20341 ccgattttct ttcggaatgt acgtaattct ttgtttttta attttgtaat atcttgatcg
    20401 tttacgatca cttcgccgct cgtgggaagc tcaagtccat tcaggagacg gactaatgta
    20461 ctttttcctg cgccagaata gccgacgatt ccataaatat cccctttttc aacgtttagt
    20521 gaaacgtcat tgacggcttg gatgtttccg tttttcccat tgaatgtctt tttgacattc
    20581 gttagttgga ttaatgacat gccattcaca tctttctctt ataatttttc aaacaaaaaa
    20641 gccttcgccc tcttgcctta agcaaaagga cgaaagcttg aacttccgtg ttaccacctt
    20701 gatttaccgt tacttcgcaa taacagcctc ttccagtact tggacttagc ttctttccgt
    20761 atactgtcgc gctgtaacgg gcactcccgt agtaagtttg cttacgctcg cttactctgc
    20821 tctaagacca tcttccatct cattttcgtt acccattttc agcaaacagg gctctcttca
    20881 aacgtccgtg acatgtactc ttcttttcaa agctttctcg tttcatatag gattgcttat
    20941 gattatagca actggaagaa tcagtgtcaa cttttttcaa gtgattttct gattttataa
    21001 ttcgtcaaat tcttttggat caacatcttt gaaacttaag atccacgcat tcatttcatc
    21061 tgaatcattc aagatattga tatcttcacc gattttatca ttaacagaag aaactgtacc
    21121 cgttagcggg ctggcaaatt cattgaccgc tttttccgct tcaacttcga tcagaggttc
    21181 atcttttttt agcgattgtc ctgcttttgg cagattggca aacgtgatgt ctccaagttc
    21241 ttcttgcgct tcatttgtca aaccaacaca gtattctttt ccattaaata agatccaaag
    21301 gttgtccttt ttcttcaaat gcttgcctgc cattttatat cagttctcct tcataaattt
    21361 tttcattgaa tccaattgca acaagctggt cctctcgtaa gatcagtggt cgcttgatca
    21421 gcataccatc tgtcgataag acttggctcg cttttgaagc cgtaaactcg cctacttgat
    21481 ctttcagtcc aagttcccga tatttcatcc cgcttgtgtt gaacaggcga cggattggta
    21541 cttgactcgt ttctagccaa gtctcaatcg ttgaggcctt cggtacttct ttgatcatat
    21601 caacggtttg ataactaacg ccatgctggt ctaaccacgc tttggctttt ttgcatgttg
    21661 aacatttcgg gtaccagtaa aaggtgtaca attttcttac ctccttgaag atcttgcagg
    21721 tgcttcattt tggtatctca tcgataatat caacccgata ccgatcatgt tccctaaaat
    21781 cgaggatcct ccctgactaa tgaaaggcaa tgggatccct gttaaaggaa gtaatccgat
    21841 atttgctcca atattctcaa agacatggaa cagcagcatc atgatcaaac cgcttgcgat
    21901 ataggcgtaa aattcattat tcgtatcgaa acacacccga atcatacgat agattaaaat
    21961 aaaatacaag aaaatgacga atgcaccacc gataaagccg aagttttcac cgataacggt
    22021 aaaaatcata tcggactcac gaacaggcac atagatattg cttacgttga atcctttacc
    22081 aaacaagcca ccggtaccaa ttgccaaaag tccttgggcc ggctgataac tcattcctga
    22141 cgtatcatgg aaagggtcca gccatgaatc aatacgtgca aactgataag atttgaatcc
    22201 aactttataa agcaagtctc ttccgccttc agtcgtaacc aagaagatcg ttcctgctcc
    22261 aacgactact gccaagatta ctacaggtgc gatgatgcgc caagagattc cagacatcag
    22321 gaaaatcccg ccaaagatcg caagaaagac aagcatcgtt ccaaagtcat cttgggccat
    22381 gatcaatgct aaaaccggta ttgtcacgat caagagcttc ccaatcagcc aaaagtccga
    22441 tttcatcgtc cgttctttta cttgcgtatt atgtttagtt acgatcagtg ccatcatcaa
    22501 gatgtacgca atcttcatca actctgctgg ctggaaagta aacgcaccga atcgaaacca
    22561 gtttttagac cctgttgaag cagctaaatt ctgatcatag aatttgagca agagtcccat
    22621 taccactaac cccaaaccat aaaggtaagg agtcagtttc cagatccatt tggaattcaa
    22681 atgcatgatg acaacgattg ctgccgctcc aactaaatac cagacaactt gcatccctac
    22741 gcctttcaag actgctggat tgttcggatc atggctaagt gctacatata atgagagcag
    22801 accaatcaaa cataacagaa agactggtaa aatcacgcca taatcaatac gattatcgtt
    22861 cgaagctttt aatttttttt ccatgtttca tcccttttct taaaaaccat actgctgatt
    22921 attatagcgg tccagttaga taatggaaag tctttccata gacatttggg aaaaattaac
    22981 tttccgtttg tgcagttgct tgagcaaatg cagtacaaaa gcctactgct tatctttagt
    23041 gtatcataga actttttttg atacatgcaa ttatctacca gctgagattc tcctcgctgt
    23101 gagaagtttc tgtgctttca ttgtatcgaa ggatctcttc ggcaatctct agcagctgat
    23161 cagaaaccag attggttcgt tggatgaaat aaaaatatcg atggaaattc aacggagtca
    23221 ttggcacatt ttctggaaca gagcgcttag acaaaatcga gcgaccgatt ccttccttca
    23281 gcaacgcaac aattacatcg ttacttttca cgatcatctt cttatcttgg atattcatct
    23341 cttcaaaata acgctgcgtg taatgaaaaa caccagagtt tgcttctctt acaagccaca
    23401 atttgctttt tgggtcccca gcgatgacta gctggtcttc catcaaagag tatctttgta
    23461 cgccggaagt ctctaatggc ttttcgataa aaccaaaatc ggattcatgt ttcgctactt
    23521 gttctaatga ttcatgagaa ttcgtgattt cgatctcaaa atgcacttta ggaaaccttt
    23581 tgatcagatg tttcatcaac tgaggcaaaa gatagacagc aaaggtgtgg gaagcaacga
    23641 tccgacatac ttcttcttca ttttgttcat tgagcaattt ttgctgagtt tcttcccatt
    23701 cttctaacaa aaccagtgtc cgttcatata ataagtcggc ttgcttagtt ggaagcatct
    23761 cttttcttcc gttacgttga aaaagatcga tattcaattc ccgctccaac tgtttgattt
    23821 gattcgagac tgctggttga gataaaaaca gtacttctgc tgctctggaa aaatttcttg
    23881 tttcatatac tgctcgaaaa gtcgttaata acttaaacat ggcaccactt ccataacttt
    23941 ttatgataat tactataaaa actatttgat ttaaatatac aaaaaaccat ctataataga
    24001 aatgtacaac aaaacagttg ctgatccaac tagtaactta gatgaagaca atcagctgtc
    24061 tgcttgctcg taatcataca ttcctattca ttttacaatg tattcgttca tcttggtact
    24121 aaaatctatt aaataaaaag gagaatatta tatatgaaga actttttgaa aattattccg
    24181 atcccgatct gcgggttgat ccttgggtta gcttcccttg gaaatctgtt taaagactat
    24241 caccatgttg ctttaggcaa tctaactggc ggtatctcta tgattcttat gatattcatt
    24301 tttgttaaat tgtttatctt attcgaacac acgaaacaaa cgttgcatga tccaatcatc
    24361 gcctccgttt caccaacttt tacgatgtca ctcatggtta tttgtactta ttttgtttca
    24421 tatgaaagta ttgcaccaat tgtcaaattc atttggctgt gcgctgttat cttccaagtg
    24481 atccttgtct tttatttcaa ttatcaccat gttatcaaag ctgatctttc aattgaagca
    24541 atttatccaa gctggtttat catttacgtt ggatttgggg tcatcactgt aactgccggg
    24601 aacttcttcc cacttattgg aaaaattttc ttttggattt cattgatctg ctatgcgttc
    24661 ttattgccga tcatcatcaa acgtgtcttt ttcgtaagaa atatggctca tccgacacgt
    24721 ccgctgatta cgatcgttgc agcacctggt tctctttgcc taacaggtta tcttaaaaac
    24781 tttgctgaac ctaattttta tcttgtcgta gcattgttcg ctttatctca agtattgtat
    24841 ttcgttgtgc tgtctatgct tccacgtttg ttcaagcttc gtttttatcc aagctatgca
    24901 gcctttacat tcccgcttgt tatctctgca acagctatat ttactactgt tcattacttc
    24961 aacagtttag gtatctatag tagcgtattg gatttcttaa aagttgcgga atgtctgttt
    25021 gcaagtgtag ttgtcttcta cgttcttgct cattacgtac gtttcttaat taaagaacat
    25081 agcaaacatg gaaaatttgt ttctaaaaaa atcgcttaat aatcgattca ataggttgtg
    25141 aaaaagctgt tctgcatcaa gtaataagaa cggccaaaca aaaacagctg ttcatgtttt
    25201 tgtactggtt gggcttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtc
    25261 gtaa
//
LOCUS       DAYYAR010000026        25123 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000026 DAYYAR010000000
VERSION     DAYYAR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.026"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACHAT0_000952"
     CDS             6..245
                     /locus_tag="ACHAT0_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450911.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACHAT0_000953"
     CDS             312..521
                     /locus_tag="ACHAT0_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450912.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACHAT0_000954"
     CDS             581..1252
                     /locus_tag="ACHAT0_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8450913.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVLAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACHAT0_000955"
     CDS             1305..3281
                     /locus_tag="ACHAT0_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8450914.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDITQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3296..4048
                     /locus_tag="ACHAT0_000956"
     CDS             3296..4048
                     /locus_tag="ACHAT0_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8450915.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4064..4819
                     /locus_tag="ACHAT0_000957"
     CDS             4064..4819
                     /locus_tag="ACHAT0_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8450916.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            4832..5092
                     /locus_tag="ACHAT0_000958"
     CDS             4832..5092
                     /locus_tag="ACHAT0_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450917.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5089..7179
                     /locus_tag="ACHAT0_000959"
     CDS             5089..7179
                     /locus_tag="ACHAT0_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFM8450918.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7206..7670
                     /locus_tag="ACHAT0_000960"
     CDS             7206..7670
                     /locus_tag="ACHAT0_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450919.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            7667..7909
                     /locus_tag="ACHAT0_000961"
     CDS             7667..7909
                     /locus_tag="ACHAT0_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFM8450920.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            7927..8229
                     /locus_tag="ACHAT0_000962"
     CDS             7927..8229
                     /locus_tag="ACHAT0_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450921.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8299..10362
                     /locus_tag="ACHAT0_000963"
     CDS             8299..10362
                     /locus_tag="ACHAT0_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450922.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10362..12974
                     /locus_tag="ACHAT0_000964"
     CDS             10362..12974
                     /locus_tag="ACHAT0_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8450923.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            12971..15502
                     /locus_tag="ACHAT0_000965"
     CDS             12971..15502
                     /locus_tag="ACHAT0_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8450924.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15552..15884
                     /locus_tag="ACHAT0_000966"
     CDS             15552..15884
                     /locus_tag="ACHAT0_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450925.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            15885..16478
                     /locus_tag="ACHAT0_000967"
     CDS             15885..16478
                     /locus_tag="ACHAT0_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450926.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            16641..18665
                     /locus_tag="ACHAT0_000968"
     CDS             16641..18665
                     /locus_tag="ACHAT0_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFM8450927.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            18665..19864
                     /locus_tag="ACHAT0_000969"
     CDS             18665..19864
                     /locus_tag="ACHAT0_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8450928.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            19880..20524
                     /locus_tag="ACHAT0_000970"
     CDS             19880..20524
                     /locus_tag="ACHAT0_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450929.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            20535..21341
                     /locus_tag="ACHAT0_000971"
     CDS             20535..21341
                     /locus_tag="ACHAT0_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450930.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21363..21593
                     /locus_tag="ACHAT0_000972"
     CDS             21363..21593
                     /locus_tag="ACHAT0_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450931.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            22107..22415
                     /locus_tag="ACHAT0_000973"
     CDS             22107..22415
                     /locus_tag="ACHAT0_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450932.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            22417..22839
                     /locus_tag="ACHAT0_000974"
     CDS             22417..22839
                     /locus_tag="ACHAT0_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450933.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            22859..24448
                     /gene="mobP2"
                     /locus_tag="ACHAT0_000975"
     CDS             22859..24448
                     /gene="mobP2"
                     /locus_tag="ACHAT0_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFM8450934.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            24460..24795
                     /locus_tag="ACHAT0_000976"
     CDS             24460..24795
                     /locus_tag="ACHAT0_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450935.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            24936..>25123
                     /locus_tag="ACHAT0_000977"
     CDS             24936..>25123
                     /locus_tag="ACHAT0_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450936.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         9386 a         3904 c         5155 g         6678 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtacttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcagaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgagtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ctatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttattggaa tgttaggaat cattgcaggc tgctttcagg
     1381 gatatcgagc atatgcagaa gatatcacgc aaaaaacgcc accagaaaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgactgaag
     1621 attctgatct aaccaacgcg aaagtggacg aaatcgttaa agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacaa acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagattac ttttgctgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gactgataat acaggttcag gttttttaaa agacattcac ttatatccta
     1921 aaaatattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcacagctc
     1981 aaagcaaaca aacaccttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attaggagat cttgtatcgg atgatcaagg aaagctgact gctactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcaga agttgtagtg ggatcagata
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacaattca tatcccaaca gacattgcag
     2401 gcggagcaga cggtatcaca gtaaacggag tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg ggatacagca ggacaaggac tttcttatgt cgctgctaaa gcaaatatca
     2521 aagtaacaaa taaagatgga tctgtggtat taaaagaaaa tacagattat aaaatccaaa
     2581 attcagaaaa tggctttgtg atcgatttta tcgtgaataa tggccaagta agtgatactg
     2641 tagcaagttt acacggacaa gatcttcaaa tgacttataa catgtatgta aatgacagcg
     2701 cagctgttgc caatccatta acaaatagcg tggatttcgt ttataacaac aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggt gcaaaattct
     2821 taaaagtcga tagcggacta tttggaacag gaatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgctgc taaaaacgca gaaggaaaat attatggtgg attagttgat acggataaag
     2941 acggtgtcaa agaagcagta tgggtagacg atgtagcaaa tgcagcgatc ctaaaatcag
     3001 ataaagaagg tcactttgaa atcactggtt taactgaagg tgaatattca ctggaagaaa
     3061 caaaagcgcc tgaaaactat caaaaattaa cgaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaacaa tcaaaaagct tccgtaccaa
     3181 tgaccggtag caacggtttt caaacgtatg tattaatctc ttgcttgttg ctaggagcag
     3241 gggcactttc tgcagttgtt tactttaaga agaaagccta gaaggatcaa aaccaatgat
     3301 tatcaggaag cctaagaaaa agcgaatcat gggtaaatgg atcattgctt tctggttact
     3361 ttcagctgta ggtgtcttac ttttgatgcc tgctgaagcg agcgtggcga aataccaaca
     3421 aaatcagcag attgccgcaa tcgatcgaac aggaacggca gcagaaacgg attcttcttt
     3481 ggatgttgct aagattgagt taggcgatcc tgttggtatt cttacaattc taagtatttc
     3541 tttaaaacta ccaatttatg acggaacgtc cgacaaaatt ttagaaaatg gtgtaggtat
     3601 tacggaagga actggtgata ttacaggagg aaacggtaaa aatcctttaa tcgcaggtca
     3661 tagtggactt tataaagata atctttttga tgatttacct tcagtaaaaa agggagaaaa
     3721 gttttatatc aaagttgatg gtgagcagca cgcttatcaa atcgatcgga ttgaggaagt
     3781 gcaaaaagac gaactacaaa gaaactttgt cacttactta gaatcaaatc ccaatgaaga
     3841 tcgagtaacg ctaatgacat gtacgcctaa ggggatcaac acacaccgtt ttctagtgta
     3901 tgggaagcga gtaaccttta caaaatcaga attaaaagat gaagaaaata agaaacaaaa
     3961 attatcttgg aaatggcttt taggaagtac tgtttttctt tcagtcatga tcattggttc
     4021 gctatttgtt tataaaaaga aaaaataaag gagggtaacc aacatgagaa atagaaaatg
     4081 gctatttgtt tgtgcagccg ctcttttttc tttcactgcc tacacacagt tgacgtttgc
     4141 agacagacaa gaaagcacgg atagcacaga acaaacaatc caaacgtcag atagcagcac
     4201 gattcaaagc agcgatgaaa ccgtcgattc gtcaacagtt gattcttcag aagaacctaa
     4261 aattgctgaa gacgatcaaa tagtcaaaca gaccattcac gttcaaaaga ttatttcttc
     4321 agaacaatta gacaaccagg ggcatgaaaa acaagaagga gtaaatggcg cgaagttttc
     4381 tgtttatgat gtatcggaca ttttacaaaa gatggatgtt aaggatctca ctactgacca
     4441 aattgaatcc caattaaaaa atcgagtgaa aaaactatcc agtgatcaat tgaagctagt
     4501 ttctaatgaa gaaacaaaga caattgatca acaaacaggt gtctttgaat tctcggtaga
     4561 agtacaggca aatcagaaac aagcttatta tattgtcaat gaatcaagtc ctgaaaacat
     4621 ttctaatagc gaggatattt tgctattaac acctgtttct gataaaaacg gtgaattctt
     4681 aaaggatgtg tggatctatc ctaaatcaga agcttcgcaa cctaaagaag aagtaaaaaa
     4741 aatcgtttct actggcgtaa agaaaaactt ctttgagaat tgttgggatt ttgttactca
     4801 cttgttttct agagattaag gaagaaataa aatgaaactg atcaaattat tattattcaa
     4861 tttttcttgt ttaaccgcag tctttttgta ctgcggttta ttattttcta aactatctat
     4921 tttgccaagc taccaactat acctaatgag gattttaaac ataccagctg cttccatgcc
     4981 gctagatatt atggaaaagc tcattaggaa tccattattt actacgatca atttgatcgt
     5041 tattgtcgga atttcactag tcatttataa aaaagagggg atgaagggat gaattttaaa
     5101 cactcgtgga caagacggtt aaaatatgtt tttcccataa tgatgatgtt aggtacattg
     5161 tttgggttaa aaacagccaa tgtctttgct gaaaaagtta ttgtcgatcc gtcaaatcca
     5221 atccagaacg taaagaataa ttttattatt ccaaaagatg ttcctaatgc aaaaacaaat
     5281 attactgtaa acggagcgtc aaaagtttac aaatatcatg ttgatgccaa caaaggtggt
     5341 tttacagacg actacgtagg attagtggag ggaaacaaaa atatacgcta tccttctttc
     5401 caaaaagaat acggtgaaac aaaccttgtt ttagaaggcg taacaaccaa caccaaagtc
     5461 aatattgact atggaaaaat cggtacgtat aacgggaaaa aagtcaatat caagctagtt
     5521 ctatctaata ttcacttata ctcggatacg ttgccgtgga atctactaga taacaactat
     5581 acaaaaactc attttcgaga cgatggctat aaaaatacga atggtgcaat gagtaaatct
     5641 aaaaaaagaa cagtactttg gatctcagac aatcttttct caggaattgt ttaccattca
     5701 acacaaatga atgtgcagct agtagccaca tatgaggacg gtagccccgt tcaattttca
     5761 ggagatacat ttatcagttt taactctttg aaccctgcag gaggaaaaag cacggatctt
     5821 aaaggagaat atgcccacta tgacaaaatg aatacgacag attggtatgt cagaaaagat
     5881 acggttcttt cagaatttaa atctttctac aacaatctga acgtggtagg tggccaccca
     5941 ggaggaagct ctaagctgac acaagcggat aatgatttca ataatttgca tgataaatta
     6001 ggagatccga agtttggtca aggtactgtg tcctttaaaa tatctgaagc aaatccgacg
     6061 tttgtgattg gttcttctaa tgttcaaacc tggtttactt tatcttctgc gacaatcttt
     6121 agtgttgttc ctgatcaacc tgaaaaaaca ggggttgata aaaatggaaa taacgtgaac
     6181 gataaaatgt tgcaagtagg agacacgatt caatatcgaa tcaaacagaa ggtgaatcga
     6241 ttaggagtgg atttactagc ggtttacgat cgatttgagt taatcgataa tttaccaaaa
     6301 gaagtaaatt acgtcgatgc acacgttgaa agtggtacaa ataaaaaatt cgatgtatct
     6361 ggtgaagtga cctatgacaa aacaaaacac caggtaaaat atgcagcgaa agctgataca
     6421 ttaaaaaatc gaatgaagta taacggcgaa acttacgaat tagtgatcaa tgtaaaagtc
     6481 aatgaattgg ccaatcaaaa tagtgtagcc aaaaatcagg gaacgtcgat cattaataaa
     6541 gtagaaaagg atacaaatat cattaccgtt tatttcccta aaattccagt gaaagaagta
     6601 caacaaaatg gaaaagatgt aaacggacga aatgacggta agaagggtac gccgacagct
     6661 cctttaaacg caggatctga agtacaatat ctagtcacac aaaaatggca cacaaaaggc
     6721 gtagacgctg cgtcagatca ctacaaacaa tttagtattc aagatccaat tgaagctcgt
     6781 ttgacatata aagaaggttc tgcgcaagta atagataaga gtaaaggaaa agatattact
     6841 tctgaaggca cactaaccta tgatagcaat tcaagaacgc tgaagtggga agcttccgct
     6901 gacttcttaa gtaacaatct tttagatgga cgagaaattc agctgatatt tacagctaaa
     6961 actccattac aatcagaaaa aaatattgat aaccaagccg tagtagcagt agataatgtt
     7021 tctaataaaa caaatgttgt gacaattggg gttgatccta acttgccaca ggtcattgtt
     7081 cctaaaacag gttctacaca tttagtaacg atttcagcgg tcagcttact attacttgtg
     7141 ttagcaactt ttagttatgc ggttctgaga tacatttaaa ataaaaaaac gaaagaatga
     7201 ataaaatgaa aaagttaata atagtagtta cttccttgat tgccatgatt gttattttag
     7261 tcggatgtag taatgaaaaa aataaacaaa ctaatcaaga taatggcgta ttgaaatcag
     7321 gaactatgtg gaaagaagaa gtaggtggtc ttgtttataa tttgaaaatt atagacgaga
     7381 ctacctggga atactctgag agtgtatggc atccagatcc tgtacaaata acagtcaaga
     7441 ggcaaaaaga ctataaaggt ttagaaagat acaaaatagt agattcagct ggtgttaggg
     7501 aatttataaa caaatctgat tctttattta ttgttgttcc ctatgagaag aatggcgtaa
     7561 aaaaaataat ttttcttgga tctagcaagg atgaaaagcg aacgaaagag aaattgattc
     7621 acgatggatc acaatccaat aaatataagt tacaaaagac tagcgagtga ttgctagtct
     7681 ttttatttgt cctgaaggag gaatcacagt ggcaaaagct aaaacgccat ttgaaaaagc
     7741 cgcgtctaaa atcttagaac gccgcggtgt atctgaaaca cagtggaaac aagaagtcct
     7801 tgaaaaagca caatttgacc tattacgggg aaaagacaag gacttagaag gatatatcta
     7861 tcaacgtgaa cgtgaaaagc ttgttttcga tgaaattatt aaacactaaa aggagcgaac
     7921 aattttatgt ttgaatcaat tacaatgaat tttttaacac aaccgaaagt agtagctgcg
     7981 gttgatattt ttagagcatt acaaactgcc gcaactagtt taaaaaacaa tctgatgatt
     8041 tttgctgctg ctgcggccgt tgcttccatt gttattgggg gtcttttctt cctatttgga
     8101 cgaagaggag ctgaaaatgg gaagatgcac gtaggctatg tattgatggg ggtaggagtt
     8161 attttagcag cagcttctat tgtggtttcg ttatttggat tgtttggaca aacacctcaa
     8221 ggattataag aaagtaaaaa agtaggagta agaagtagtc gatacttttt actccttttt
     8281 tatgaaagga gaatactcat gtactttacg attgcaggca ataccaaaac agataaagag
     8341 ttaataaaaa aagcaacgaa ggaagtagga aagcgtactt cttttttacc gaaaacaatt
     8401 aaatattctg tcattgaaac ggaagaacag ctacatgtgt tagggaaagt actggaacac
     8461 gaaagtcgat cttctgttaa gttgactctc acgttacatg acacagaagg aaaaatagag
     8521 aaagctggta cagaatacgt ttttggccca ctggattatc aaatttcaga aggtttctat
     8581 cctacattga tggatttaat ccgccttgat gtgtttaaag aaacggatga aactatgagt
     8641 gtggaagagt cttatcgaaa cgaaaaactt ttacaagttt tagaagaaga gttagagagc
     8701 gaccaaagaa aagtgaatca agggagtgaa gtcgaagtaa atgaagaagt accatgggaa
     8761 cgaccaatat ttccaaagaa ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa
     8821 gctgatattc cgactcagaa atcgatattt gaggaagaga acgatgtgat gggtcaattg
     8881 gaggtagatc cctttgaaga aacaatggag gatgaagaga cggccgaagt agaagttgat
     8941 ccagttgaag cgaatgaaga gtcaacatgg actgaagggg attctcagga atatattgaa
     9001 ccacttatca gtgaagaagt agaaaaaaac gaaatagaga tacaaaaggc aactgtattt
     9061 gattatattt tggatcaata tccgccagag catgaatgga tgaaagaaaa aaacaaatta
     9121 tttttaaatg agctttattc gaaatatcaa ttaccccaaa cctatcaagc atttttagag
     9181 agtaaagaag aattaatctc acagggtaaa ggcacacttt tagaacagtt agagtctgtc
     9241 caatcaacaa attggcatga gcttgcacaa gaagatttat tagccacctt tgaagaacgt
     9301 caacaagaaa cagatcaaga aattaatgag tatgcgacgg aacagtcgtc taattgggag
     9361 aaagcaaaag aggaactaaa taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa
     9421 attaaagagc gatacgaaaa gaaacgcgac aatgcgtatt ccttgtgtgt agcaaagatc
     9481 aataatttta ctcagacgaa tcaagaagcg ttatcgaaag aaaaagaaaa cttgttaaaa
     9541 gaacgtatta aagaacgaaa agaagaattt taccgaggtt tgcgtacaga taagctaaag
     9601 attagcaaag aattaaatga acaactgaat aagctatatc aaacaacgac tgaagttctt
     9661 atggaaaaat acgaaaagat ccagcaagaa ttacaagtgc aaattccaaa atggaaaaaa
     9721 gagtatgaaa ttcaacaaca agaattgaaa gaacaagaag aaaaacaacg gcaaaaagaa
     9781 aaagagcgcg aacaattaga actaaaaaaa cgtgaacagg atcttgcgga acaacaaatg
     9841 gcaatagaaa aacaacggtt aaaccaagaa aaggaaaaag aagcagctgc tcgtaaggaa
     9901 cgtcaagaac aaatggatac attgcgacaa gctcagttaa attatctagc acaacctcaa
     9961 ccaacgcctt cagtagctgc tgttcaaacc ccaatgaaca accaaacaaa tctaactcct
    10021 tcaccacagc gttcctttaa cggatggatg gtgggatgta ttgcaagttt aagcttgctt
    10081 ctaggtggag gaacagtatt tgcttttaat catatcaatc aatcttcaca ggctgattct
    10141 gtggccacaa cgacgtcaat caaggaagaa gcccaagaac aagcaagaaa agagtatgaa
    10201 caacaattag aagaattatt gaatcgtacc aatcagtcaa caacagagtc aagcatggaa
    10261 aatagcaaag agagtgattc tagtaaggct aaagaaggat caagcgattc aactgtacaa
    10321 tcaaacactt ccgaaagttc agaatcaaat gaggtgaaat aatgcgtgtt agagaaacaa
    10381 cgaagcgaaa agacaaacga ttcaatttag aaaagaatag gtatctttct catttctatc
    10441 gctatactcc ctcaggagaa gagctaccag aaaaacatga caaacgcatt tcgaaaaaga
    10501 ttttttatag tggacttgta tacctccctt tagagtattt agtaacaaga gtaccagaag
    10561 ttttcaaccg attggaaatt gttaagggaa gcttacaagg aataaatctg tttcaattac
    10621 aggaaatgcc acaggtcttt cattttgatt cagctggttt ttggaatcca tttactccta
    10681 caactagttt gggtacacgt ttagcaattt cgacagtttg tgtggttcca gctgtactta
    10741 tgggattgaa aatggacatg tggtttcgtc ctctagcaga tggacaaaaa ggggataatc
    10801 gattaatgac cttaaaagaa atccaacaga tttatccgca aatacctgaa acaaaaacta
    10861 cttttccagg ttatggaggt attccaatca cacattacaa aaaatattgg tacattcaaa
    10921 cagatactgt taatacgtgt attgttggta cctctcgatc agggaaaggt cagattgaag
    10981 tgttagccac cattgacaat ttatctcgtg cagaaaaaca aagttcaatg gtcgttaatg
    11041 atccaaaaac agaattatat gttggtagta aagatgaact agaagctaga gggtatgatg
    11101 tatatgcctt taatatcacc gatccactgc aatcaatgag tgataatcca ttggcactca
    11161 tcgtgaaata ctggaaacga ggagacatag atactgcaac acagttaacc aatactttca
    11221 caaatacgat ttattatgat gccaacgctt cagataacaa atactttaac gaaaacgcgc
    11281 agaaagctgt caatgcgctt atttttgcgt tgttagaaca agctgatcaa acaggagatt
    11341 actccaaatt gacaccgaat aatctcgttg agcttctttc tgagattggc ggttttaatt
    11401 attatccaga tccagacaat gaattcaaac agttgaatgc cttagatgaa tttatgaatc
    11461 agttgccacc tggaaacatt gcaaaaaaac aatatggggc aacgaaaatt ggatctgata
    11521 aagcgaaggg aaatatttta tcaacggcaa tcacgggatt gaatccattt actctaacga
    11581 aaattgcaaa gatgacttct caatcaacga ttgattataa atcggttggg tttccaaaat
    11641 acttagattt gaaattacat gaaagtcttt taaatcaacg agtcgtagta gaattttatc
    11701 ataagggaaa gaaatttcat gaagaacgtg tcaaagtagg ctataaagga ttttgtgaaa
    11761 tcaattttga ttgtcattta gttgcaggtg atcaagtttg tttaaaatac gatcatcaag
    11821 gaaaagaata tgtggcttgg tatgaatttt ctcaaagagt attgaaagac gaagagggta
    11881 atatcgttta tcggaaaaaa cgaggagaaa cccatttgcc agaattagaa aaagaagccg
    11941 ttctaacatt agatgaggag aaaagtaact tattagtaga gtctattcgg atgcactata
    12001 ccgataaacc aatggctatt tttatgctta cacctgatta tgatccatct aaccatatca
    12061 ttgttagtat ttttcttagt cagttatata aagaattatc tacgcaatgt gttaagacta
    12121 aaggagataa atgtcaccgc aggattcatt ttattttaga tgaatttggg aatatgccac
    12181 caatggataa tatggaagga atcatgacgg tcacagctgg ccgaaacatg ttgtgggatc
    12241 tgtttattca atcttatcag caactttatg cgaagtatgg agaacaaaac gcagcgatta
    12301 tcaaagaaaa ttgtcagaca catatttata ttatgtcgac agatgataat accatcgaag
    12361 aattttctaa aaaagtgggg aataagacgg ttgaacaaga gaacagtacc atcaattcat
    12421 taggtatgaa tactcatatc aatcgcaatg ttgattcaga tcgtatctta actcctgaaa
    12481 gaatttctac ttttttagaa ggtgaaacga ttgtactcgc tcctttaaaa cgacaagatt
    12541 tgaaaggtca taaaatacgg ccgtatccta tttttaatca tggggaaacg aatcttcctt
    12601 atgcttatca atatattgaa gatttcaatc catcgtggga tatcaatgat atagacatac
    12661 attgtacaca tgcggacttg gatcttaagg ctttacaaat tgattatgat tatttcctac
    12721 cagatgaata tgctagaaat ctctatcatg gactgcatga tcaattagaa gaaaaaaacg
    12781 aagaaaatat gttttataaa atcgttcaat ctcttgagaa tgagacggct aaaaaattgt
    12841 taattcaatt ttacgaagaa caagcagcag atcgacttca agaatatgtt gaaaagttga
    12901 tcaaaaataa agaaatttct gaaatgattg ggaaacggtt gttattggaa tctgaaaagg
    12961 tggtgaaaga atgatcattg gaggaatttt ttctggcata gccgactggt ggtcaggctt
    13021 attcggagaa ggggatgcaa accccaaaac cgtaacgaaa gtcttacaag attatggtaa
    13081 ttatctgcag tatcaagatg tatttagttg gatttggaac acttttctgt ggggattgat
    13141 taaaggatta tataatctca atactttact agaaaaaacc atttatcaaa gctttgattt
    13201 aaaagactta ttgaacgccg ctggggtaaa tgcactattt caaagtttga ttagtaaggt
    13261 attggctctt ttttgtgtcg tcacattaat ttatttaggc ttgaaatttt ttatgagtaa
    13321 acacccacca aaagtcaaaa atatcttggt taatttattt atggccatga tcttgatttt
    13381 tggtgggagt tctttaatcg atgaagggtt aaatatcagt aaaagctttt acggagacgt
    13441 cactgcagca aataaagcag ataaaacggg ttcacctgct tttcaaatta ttcaaaataa
    13501 tgtgatcgat gtcagtacat tactagcaac tgacccgaat aaagtagaat ccctgccaac
    13561 agacaaaaga aatgctttaa cagcgaaaaa ttttaaaaca gcgaatatca atgggattat
    13621 tactcctaaa caagctaaag aaatggcgga taaagataag agtggtttat cagatttaca
    13681 aaaggatcgg ttaagagcgt tacaataccg attggaacta gatgataatg ggaaagaaat
    13741 tcctgttcaa attagtgacg aaggcttagc aaaatatgtc tatacttcag ggtatcgtcg
    13801 atacagtagt aaaccaggta ttatcttagc tggtgaatct tctttggcgg tggcctattt
    13861 atttatccta tttacgatcg ttacttgcat tattgagctg gtatttaaaa aattctattt
    13921 agtgattgcc gcgtcaacgg actttgagac aggccaacgg atgaaaagtg cgatagaaga
    13981 tatttctcaa tccttcttgt tattggcgtt tacgatttta gaattacgaa tctatacatt
    14041 aatgctttca ggtatcggtg atttgcatgc ggcaggaaag atcaatggat tcttgtatgt
    14101 tgttgcttta attgtgctaa ctattgcgct attcaaaggc tctcaatcgg ttacgaagat
    14161 tttcggtgtt gatacgagtc tgaaaaacgg cagtaattct cttcttagca tgtttgcctt
    14221 aggaaatatt gcgaaaaatg tgggtagcgg agctaaaaac cttgcaagtg ctggtaagag
    14281 tggtttaagc catttaaatg atattcgtaa aaatggattt ggccatcgaa aagatgttac
    14341 ccctgataac ggggaagaac aatcagacga taaagctaca actgtaccta atagtaagaa
    14401 aacagtttcc gaaatgttcg ggaaagcgaa aaatggtgtt tctaaagccg cagaaggtct
    14461 tggttacgtc aatgaacgtg ggttaaaagg aacagctgaa gatcttacag aaaaagccaa
    14521 agacacctta gaagagaaat tagacggcac gagagctaaa gaattggctg aagctgtgaa
    14581 gaatccatcc gaagcactag aaaaacaaaa agaaaaagct tcagaagtca aagaaggact
    14641 aaaagatgcg tatggtgaag gacaagttaa tgcagcagta aaagctaata caacaaccga
    14701 aaaagaaaat caagaaggta tagtgattcc ttcagtcaaa gatcaccaag aagcgttgca
    14761 ctctaatgaa aattcgattg aagagaaaaa ttccatagaa gaaagaaatt cagaagaagg
    14821 aaaaaatgga cagttaccaa aaactgatcc agaacaatat cgctataaac ctaacagtga
    14881 ccttgaaaat gagaagcgaa tttcagattt atcaaaagta aatcaagaag acaaagcaga
    14941 aaatgaggga cagttaccaa aaactgatcc agaacaatat cggtatagat ctgttaactc
    15001 cgaaaatgaa aaacgcattt cagatttacc aaaacgaaat caagaagtac aaacggatga
    15061 ggattccatc gttctgccaa atgataatgg aatcgaagtt ggaactgaaa aatcaacgaa
    15121 aattggttta cctaaggcac aaaatttacc aatgaaagat agtcgtttga atcaattcaa
    15181 ggagaatgat caaagctttg ttcaccaatc aactgcaggt actgttcaaa tgaaaccaag
    15241 tcgtttgcct gaaaatggaa atctgacaac tgtttcttac aatttatctg aaagtggtga
    15301 gacagggaat gcgtccgttc aaaatgttgt gacaagagaa gctgcaccaa aacctattca
    15361 aaacgttcat acaggttaca agccagcaaa tgtggcattt actgcaccaa caaaaataaa
    15421 tgtgacaaga gaaactaaaa acaggaacaa tgcattgaaa gaacaactat tgagggatat
    15481 caaacaggat atcaaaccat aaaaggccta caagttaggc cttttattta tatgaaaagg
    15541 agaaatcaat tatggataaa tatataaaca tgaaaaaccc tccaagattt agtggagaat
    15601 ggaagttttt cgcttttttc tttttagatc tattaacgct tggtggctgc tttttttggg
    15661 cgaaaatggt caataatctt gtccacttac cactcttttt tgcactcttg aattatggct
    15721 tatggattgc gatagcgtta gtgattgttt ggcgacccta taataaccca ggtcaacgac
    15781 aaatttttgc gattatcgag tctatttttt ttgcggacaa gaaccattat catgcgttag
    15841 atcctaaccg aaattatcat ggtacgtata aggaggatga ataaatgcga gtcaaaggac
    15901 aaaattcagt tgaaaggaaa tccattttgc agtttggaaa accagaagag cgctctatct
    15961 tggacttatt tccgtataaa tcctgtacag aagagggagt cttaattact agagaagatc
    16021 attttcaacg ttattatcgt gtagtttcta cagacgtgga aggattaaat gaacaagaga
    16081 aactagaacg aatgaatcag ttgaccaccg tcatgcgtac gtatgtgcca acaattaagt
    16141 tagttagttt gaccacagag acggatctaa cggaacaaat cgtgcaaaag cggcaattat
    16201 tagataaaaa tcgtatggaa cagtccttag gaagaaatct acaacgatta aaaaaatatg
    16261 aaaagaaatt agtcgaagaa atccaggaat tgaaacgagc agaacaagaa cgtcctgact
    16321 taagcttctt tttcgtcatt gaaggaaaga caatcaagga tttacaaacc agaaaccgtc
    16381 agttaatgcg atctagtggc attttagggt taaaaccact ctacaaaaaa gaattgatca
    16441 agattctgta tcgaatgaac aatatgaatg atgagtagga atggagaaga gagaatgaaa
    16501 tatccgttga tatggttaga aaaagatgtt ccgaaggaag agaccactag aaaatttcct
    16561 cacactttaa gcctgggtaa gttgggaaaa gggcaatctt ttaaccaagt aaagaatcaa
    16621 acaagaagag gtgactgtac atgttagaag caattaaaac agctttttca ccacaaaatg
    16681 atcaaaaagt acttgatcca ttaaagcaac taggaaatcg ggaaatagaa attttaaaat
    16741 ccaaagggta tgatttagat tttttaaaac aagtgatgcc acaaggtggg ttaaattttg
    16801 atgaagaata tgtgacgtat ggtaatggtt attcacgatg tgtgacagtt tatcactaca
    16861 caaaaaatcc tacgcttttt tggttggcca atctcatgaa caatcctcat acagtggcca
    16921 cactcgatgt tagcacagat gaaaagcaaa aagtgattga tcaaatcaat cgtgccttag
    16981 atgaattaaa tgatcgatca gataatgaac gaaaagcgac tgaccgaaat aaaagcgcag
    17041 aagaatacct ggaactagcg gaatatgctc gtaaattgac ttctaatggg gaaatttcta
    17101 agcaagtcaa agtgagagta catgtttacg gaaacacgat tcaagaagta gatgaacgag
    17161 cgcaggaact gatcgaaaat ctaaaaggga tggattatcg tgcggtgact tatctgcata
    17221 aaaccaaacg agattggcaa tcgatgttta ccatgttcga tgaacaagct gaatggttcg
    17281 gaaatatcaa agggcaatca ctaccatcgt taaacattgg cggaggtttc ccgttccacc
    17341 accaaacgct aaaagatcca ggagggacac aatatgggga aaccatgaca ggcggcccat
    17401 ttgtctggaa tgcgacgtat aaaaatagtg tacggttgtc tactaatacc ttgcttctag
    17461 gaatgatggg agctggtaaa tcgaccgttc ttaaaatgat cgcggaagcc catttagctg
    17521 caggagactt tatttggggc tttgaaaagg gaaaagactt tattcctttc ttaaaggaat
    17581 ataacggtat tatggtgcgt ttagacggat ctgacggaat gatcaatcct ttagagatat
    17641 ttgccacacg gacgtatgat gaagcatcca gtttatatga cgatggatca gtcaaagatt
    17701 tgaagatcaa tgaagcagcg tcctatcaaa cacacttaga caaagtggtc tatcaggtac
    17761 agttagtgag tcctcagcta aaaggaacga tgaaagcaga atttaagacc tacttaaatc
    17821 gtttttatga agagtatgga acagtaccac gcggctttac ttcctcaaat tcgcgttcaa
    17881 atacggagac gcaagtcaca ggtaaagatc cagaagctta tccgacgttc aaagaatttt
    17941 tagacttttt aggacaactg gaattaccag gtgcttccca agagaaaaag aatcgaaaag
    18001 aagaaatgga atccatcgta gaatccttat gtgagacgta tggcatgatt tttgatgggc
    18061 atagtacgat tcgacacctt gaccaacaac agttggtctt ttttgacatt gactctatta
    18121 caggcttaga tcgaggggta caacaatgtc aaatgtacat ggctatgacg ttgatttgga
    18181 atcaggcatt gattcaagga agaagacaac gacgtttaat taaagaaaaa caaatggaag
    18241 aacaagactt caaacgcttc ttggcgatga ttgatgagtg ccataatgcg attaatccag
    18301 attttattga aacggtaaat tatgtcacaa actttcaacg agaaatgcgt aaagtagaag
    18361 caatgactgt tttagctaca caatccccac aagaaatggt gccagaaaat atgtcttctg
    18421 ataacttctc gaaactgaag aaagtctttg aattttcttc tacgaagatc tttatgcgta
    18481 tggatgaatc tattttacgt catatagaag gattagtggg taaatcaatg acgaactcag
    18541 aattagaatc cattcctcaa caaaatcaag gagacgctgt gatcaatttt ggctcaaaag
    18601 aaaccattcg tgttcatttt agtccgaaca aacgtcaatt aaaattgttt gatggaggaa
    18661 aataatggaa aaccaaaatg aaagtttaat caaacagtat gttaagcgtc gtgcaaagcg
    18721 gcgcttattt ttgtgggtgt ttggtacttc tgcaggtttg attactatat tgatcactgt
    18781 gtttgtgacc ctttttctaa tacttgcggc aggttctatt gataattctg attcaagtag
    18841 tggtggcgaa gcttttacag gagaatattc ggaaggctta cccatttata aagaaatcaa
    18901 aggaagaggc cctttttctg atgagattgc gcaatatgct gtaggtgcag cagtgaagta
    18961 taaattatta ccaagtgtga tcctttcgca atacggttat gaatcagcgt ttggtacgtc
    19021 ggcgtccgca agaaatgatc tcaattattt tgggattacg tggtttgatg ggtgtctgtt
    19081 tcctaaagga actgcgcgtg gaattggtgg cattgaaggt ggctggtaca tgaagtttcc
    19141 caactcaaaa gctgccttct cctattacgg ctttatggta gccacacaat caaattttaa
    19201 tgcttgtgtc ggaaataaaa gccctggcgc aagcttattg atcttaggac gaggagggta
    19261 tgccgccgca ggaattactg aagacagtgc ctattataaa aattgtatgt ctattattaa
    19321 tacaaataaa ttgatggagt acgatgagtt tgcgattaaa cactggagag aaggcggcgg
    19381 aactggtggt acgattacag gaagctgggg caatccattc ccaggaagta gcttagataa
    19441 aaattcgttc agtggaggac aactttttgg gaaaaatcca ggaggagaat tccgacctaa
    19501 cgggttccat gatggactag actttggttc agtcgatcat ccaggaagtg agattcatgc
    19561 ggttcatggc ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt tgggtgcgtg
    19621 tgtgatcgtg attaattacg atggcttgaa tatggtgtat caagaatttg caaatagtac
    19681 aggaaattct cgagtgaaag taggcgatca agtgaaagtt ggtcaagtaa ttgggatacg
    19741 agatacagca cacttgcatt taggatttac tcgaatggat tggcgtcaag cacaaggaca
    19801 tgcattcatt gatgatggta catggatcga tccgttacca tttttgaata gcagcaaaaa
    19861 atagatcagg agaaaaaata tggaaaataa aaccttaaaa atcgtcttaa cagctgcaat
    19921 tagcgttagt ttaatcggca atattgcgtt gttttggcag cttaaagaag aacaagagac
    19981 aaatacctat aaaattgaag aagtggctaa aaacgcgtct gataagctca aaaaaaattc
    20041 ttctgaggca caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga aaaaccaaga
    20101 aatcgatcag ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa ctgaagcaca
    20161 aagaaagacg gcagaagagt tcggtcaatt ggccattgat aaatcattaa gcagaaatga
    20221 attaacggaa aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat taagtcctgc
    20281 agacgatgat caccaacatg acgactacgg aagttactct tttacattag gtgacgtgca
    20341 aacttacgct caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat ttgttgatta
    20401 tacaatcggc aatcctcaat ttaaagatgt caaaccacaa aaaataaaag gtggattaac
    20461 tgtcactgaa aaacaagtag atggtcaatg gaaagtcaca gactttagtt atttcactcg
    20521 ttaggaggaa gaaaatgagt aagaaaaaag gagcaagaca acgttttaaa aaacgaaaaa
    20581 gttatcttag atggctgggg atctttatga ttctcggctt tttgattctt gctagtccga
    20641 tttacacaca taaaaaggta gacataccag atgaagtaag cacgaacgca tttttaaaca
    20701 cgaaggaact gacgatggtt tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat
    20761 ttgcgatcga cgatgaagat cagagcttat taaaagaact agctaatctt aattatcgtg
    20821 tagaagtaaa gacagctaaa ggggattatc aagcaataaa agccaaagtg atcaaggtgt
    20881 ctgatgatta ttttgtagtg aagttagcac atgtaccaga agaatatatc gccatgcgtt
    20941 taaccattat tcctgaaaaa attgatccaa aagtggatat gcaagaacca caagatctga
    21001 tttattacgt tcacgaagat aaggttaaag atcaagtgaa agatgaagat tatgaacaac
    21061 atgcgatcaa ttacaaagtg aaaggataca aaaaagaaca aaaagcagca caaaaacaaa
    21121 tggaatcctt ccaagcaacc attgatttga atgaagaatt agtgaagaag ctaaaagatc
    21181 aattgcctta tcaagtcgca gatgatcagg aaaatactga aaacaagatt aatagctacc
    21241 agcaagaaat tgaaacatcg aaaacgcaaa tgaaagatca agaagagatc attcaaaaac
    21301 ttcaagaaaa aattgatcgt atgacaaaag aaaagattta gaaggacagg tggagtgaaa
    21361 aaatgaagcg aacaaacttg gctgacaaag atactttcgc tgagcagaaa gtattgatta
    21421 aagcctatct caaagttgta aaagctaaag aaatatgtcg agaagtgaat ttatctaata
    21481 caacaatatc tgagttgaag aatggtcgaa agaatttaga tactttaaaa ttcagatcat
    21541 ttgaagctct atatgtagct gctaaaaaat attttacagc tgataacaaa taaaaaacag
    21601 gagtatttta tgtgaaaaaa gtatataaaa aatgcttatg aatcagctat atcaccttaa
    21661 ccatttgtta atgagatggg agaaaacatt aaccacgatt ctaacgcaaa catcaagcac
    21721 ataaaacatt taacactcct cctttctggc tgaagatttc aaccaaacag gaagacagcg
    21781 tatcaattat agcatacact ttttcttaaa ttcatttttc atatgacaaa atatttcctt
    21841 ctaatcgaca aagttagaag gattttttat tgtctaaata cacaaataac cttaatttat
    21901 aaagagcata aggaaacgtg ggaacacttt ttaaaaaaag tgttccaata ggctgtgcct
    21961 attacgtttc tcaaaaataa ttgtgggaac aaaatattta aaaataggat agtgaaagga
    22021 tagcaaagga tagtaaaagg atagaagcag gatagcgaaa aggataggct ccttttcgct
    22081 atccttttta aattggagga attaatatgg aagtaagaat aagaaatgta gatgagaaaa
    22141 tcgtaagaga aattgatcga agatgtgacg aactaagtaa ccgaacgggt aataaatgga
    22201 gtcgtaacga ttatttaaag ctactcgttg aaaatgattt tgatcgtcct ttgatggaat
    22261 ataaacaaga taaatttgat caattattaa atcagttaat agataaattt tcttttgttc
    22321 aaagtcataa tacaaaagta ttagaagaat atatttgtca gaataatcaa attatcgaac
    22381 ttttaattga acaaagtaga ggaggagaag tttaacatgg ccaaagttat agacaagcat
    22441 atttattttt atgaaacaca tatagatgtt ctagatgaaa ttattgcaac tcatgcagag
    22501 gttaaaaact atagtgaagc cgttcggttt ctatgtatga attatgattc agaagtggag
    22561 aaaaaagtaa attccaatca agcaaagttg aatgcaatgg gaaaagaaat ttctatttta
    22621 accgaaatgg tttcagagtt tgcggatctc catttgaaag atacagggat tttaattggt
    22681 acaaattcaa ctgtttacca agaagcaaaa gagcgtgtgg aaggcaagat aaaagcaaat
    22741 caaacgaaga aatattctcc taagataaag aaaacaataa gcgaatcagt gactacaaaa
    22801 agtgaagtat ttgatccaac aaggttctta aaacgataaa aattagaagg agaatgtgat
    22861 gagtaaatca cctgcagtca ttctcacttg tcaatttacc ttgcccaata aaaaatcttt
    22921 ttcaacatac attgattata tgacaagaga aaaagcgctg gaagaaaagg aacgacgtac
    22981 gcccgaggaa gaacaggaac tgcgtctaat caagaatgct ttagaggatt tttatattcc
    23041 tgaaggagaa actttttcgg agagtaagac gggtaaagaa ttcagcgaaa cgtcgaatga
    23101 agcgcaatcg ttgatgaaac atggaatcaa ttttgacaag ttagaagaac aagattttac
    23161 caaatatctt tcttatatga cacgtcacta tgcgttagaa caaaagaaag tactgacgca
    23221 tagtgaacaa aaagaatacc aacgcttgaa ccaagcaata aaaaaatatc aaacagagcc
    23281 acacaaaaaa gcttcaggaa atcttcctgg agttttttct atggcttcag acgaagtgaa
    23341 aggaagtgat ctgaaggaga tcaagcaaac cttccaaaga ggacaacaaa aaggctcaat
    23401 catttatcaa gatgttgtct ctcatgatca ttcgtatctt gaaaaaatag gattgtacga
    23461 tcccaaaaca gatactttag atgaagaagg tttaaaagca gctggaagaa aaatgatgga
    23521 gacattgttc gagaaagagc aactgaatga aacaggctac tggatggcaa cgattcaccg
    23581 aaataccaaa catatccata ttcattttgc agttgttgaa aaaaaaaata cgcgtaaacc
    23641 atacactgag attgaaaatg gtgtgagtta tatcgtaccg aaagggaaga gaaggcaagc
    23701 aacattagac gaaatgaaga ctaattatgt acatgagtta gaacgttttt cttatgaaaa
    23761 aaatcgaatg ggtatggaga aacgaaattt attgacacgg aaaagtgatt tgcgtaacac
    23821 cttaaccaaa gtagtgaaag agccagtacg ttatgatcga cacgcaatga gcttattaac
    23881 tgaagtatat aaaaacttac ctaaaaagcg ttcggaatgg cattacgggg atgaaaatcg
    23941 aaccaaactt tcaccataca caagagagaa actcgatgag ttaacccaat atgtgttaac
    24001 aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagcaaa
    24061 aagtctttat ggggaaagca agagagaatc aaaggatgca gaaaaaaatg ctctctttga
    24121 tttaaaaaag cgcacaggaa atgccattct aacagaactg aagaaacagg atagtcaaat
    24181 aaaaccttta atagaaagga tggatcagat gcaatctatt tacaatgaac gatcctttcg
    24241 aacacttaaa cagttgccaa cgaatgaata caaatatagt tacgaagaat tcatgaagcg
    24301 caggaaacaa ttccaacaaa gagttgtcac gcaacggagt ttgcgtcaat taaatcgaac
    24361 gattaatgcc catgaagaaa aataccacgc aagtaaagca tacgaagagc aacaacgaag
    24421 aattcaaatg gaacaagaaa gacagtagga ggaatagcaa tgaaacaata tttagatcaa
    24481 tggaaagtta tagaaggatc attaagagaa gaacgaatcg aacaacttcc cgattgttta
    24541 gaaaaagagc atttgtttca gataagagaa atgttgataa acgaacagtt tgatccaaat
    24601 caatttttag tcgttgagta cccagctact ggtgtgtact gttgtaatca tgtgaagggt
    24661 gaaaaatatt ttatcattca agaatatgag ggcaagttag cgccttatta tactacttgg
    24721 gaaatgaatg aagaggggat caataacttc ccgtgtaaaa gcattgaaga gtctatttca
    24781 ctcacagaat gttagttgtt aagtgaataa aagaagtaaa agagtcctga acgtgattgt
    24841 ccaggactct tttttttcac tgaattgttt tccgccaatt cattgagtgg tgcgatttcc
    24901 gtctacatta tatcaaaaga aaggaggaag aagaaatggc ttattcaacg attggagttg
    24961 ttgttgaaaa aagcagagac aatcttgttt ttgtgacaga aattcaaaca ggcagagctt
    25021 ttgtagtcac agacaaagcg gcaaaagcat atcaaagtgg cgatatttta acgttaaatc
    25081 tgactacgaa gacgttcgtc gatgcggcag aagattatcc att
//
LOCUS       DAYYAR010000027        24255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000027 DAYYAR010000000
VERSION     DAYYAR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.027"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..1337)
                     /gene="purB"
                     /locus_tag="ACHAT0_000978"
     CDS             complement(42..1337)
                     /gene="purB"
                     /locus_tag="ACHAT0_000978"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8450937.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1350..2474)
                     /gene="purK"
                     /locus_tag="ACHAT0_000979"
     CDS             complement(1350..2474)
                     /gene="purK"
                     /locus_tag="ACHAT0_000979"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8450938.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2467..2955)
                     /gene="purE"
                     /locus_tag="ACHAT0_000980"
     CDS             complement(2467..2955)
                     /gene="purE"
                     /locus_tag="ACHAT0_000980"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8450939.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3371..4678)
                     /locus_tag="ACHAT0_000981"
     CDS             complement(3371..4678)
                     /locus_tag="ACHAT0_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8450940.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4681..5262)
                     /locus_tag="ACHAT0_000982"
     CDS             complement(4681..5262)
                     /locus_tag="ACHAT0_000982"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8450941.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5378..5475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5834..7126)
                     /locus_tag="ACHAT0_000983"
     CDS             complement(5834..7126)
                     /locus_tag="ACHAT0_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8450942.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7123..7746)
                     /locus_tag="ACHAT0_000984"
     CDS             complement(7123..7746)
                     /locus_tag="ACHAT0_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450943.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7749..8585)
                     /locus_tag="ACHAT0_000985"
     CDS             complement(7749..8585)
                     /locus_tag="ACHAT0_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8450944.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8602..9489)
                     /locus_tag="ACHAT0_000986"
     CDS             complement(8602..9489)
                     /locus_tag="ACHAT0_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8450945.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9505..10788)
                     /locus_tag="ACHAT0_000987"
     CDS             complement(9505..10788)
                     /locus_tag="ACHAT0_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8450946.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11471..12154)
                     /locus_tag="ACHAT0_000988"
     CDS             complement(11471..12154)
                     /locus_tag="ACHAT0_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8450947.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12172..12789)
                     /locus_tag="ACHAT0_000989"
     CDS             complement(12172..12789)
                     /locus_tag="ACHAT0_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8450948.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12799..13623)
                     /locus_tag="ACHAT0_000990"
     CDS             complement(12799..13623)
                     /locus_tag="ACHAT0_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8450949.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13616..14176)
                     /locus_tag="ACHAT0_000991"
     CDS             complement(13616..14176)
                     /locus_tag="ACHAT0_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8450950.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14350..15306
                     /locus_tag="ACHAT0_000992"
     CDS             14350..15306
                     /locus_tag="ACHAT0_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8450951.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15611..16567)
                     /locus_tag="ACHAT0_000993"
     CDS             complement(15611..16567)
                     /locus_tag="ACHAT0_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8450952.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16671..17273
                     /locus_tag="ACHAT0_000994"
     CDS             16671..17273
                     /locus_tag="ACHAT0_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8450953.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17361..18860)
                     /locus_tag="ACHAT0_000995"
     CDS             complement(17361..18860)
                     /locus_tag="ACHAT0_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8450954.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18876..19400)
                     /locus_tag="ACHAT0_000996"
     CDS             complement(18876..19400)
                     /locus_tag="ACHAT0_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8450955.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19698..20447
                     /locus_tag="ACHAT0_000997"
     CDS             19698..20447
                     /locus_tag="ACHAT0_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8450956.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20438..21223
                     /locus_tag="ACHAT0_000998"
     CDS             20438..21223
                     /locus_tag="ACHAT0_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8450957.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21192..21908
                     /locus_tag="ACHAT0_000999"
     CDS             21192..21908
                     /locus_tag="ACHAT0_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8450958.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22010..23029)
                     /locus_tag="ACHAT0_001000"
     CDS             complement(22010..23029)
                     /locus_tag="ACHAT0_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8450959.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23048..24049)
                     /locus_tag="ACHAT0_001001"
     CDS             complement(23048..24049)
                     /locus_tag="ACHAT0_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8450960.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7620 t
ORIGIN      
        1 ttgctattct attttaattg aattttgcaa ctcactcttc tttattccaa acctactcgt
       61 ttgaatatca cgtcaacgtt tttcaaatga tgatgataat caaaggcatc gtctatttct
      121 tctttagata ataccgatgt gatggttgga tcttcttcta acagcgggcg gaactgcact
      181 tggttgtccc aagcgtacgc agtttttggc tgaacaagat cataagcttc ttctcttgtc
      241 attcccttat cgatcaattt cagcataacg cgctggctat aaatcaaacc aaatgttgca
      301 gacatattcc gcttcatgtt ttcaggaaag accgttagat ttttaacgat attgccaaaa
      361 cgatttaaca tgtaattcaa taaaatcgtg gtatcaggca agatgattcg ttctgctgaa
      421 gaatgagaaa tatctctttc atgccataat gtgacatttt cataggcagt gaccatgtgt
      481 ccgcgcacca ctctggctag accagccata ttttctgaac cgatcggatt ccttttgtgc
      541 ggcatagcag aagaaccttt ttgacctttc gcaaaaaatt cttctacttc acgtgtttcg
      601 gatttttgca atccgcgtat ctctgttgca aaacgttcaa tacttgtagc aataagcgcc
      661 atcgctgaga aatattcagc atgtagatcc cgagggagaa cttgagtaga gatttcttgt
      721 ggacggatcc ccaattgttt acatacatac tcttcaataa atggcgggat atttgcgaac
      781 gttcccactg ctcctgagat ttttcctgct tctactcctt ttgccgcatg ttcaaatcgt
      841 tcgatgttcc gtttcatttc tgaataccat gtggcaagtt tcaatccaaa agtagttggt
      901 tcagcatgga caccgtgtgt ccgacccatc atcactgtat atttgtgttc ttttgctttt
      961 tcgccaatga tttccgtaaa acgtttcaag tcttcccgta aaatatcatt tgcttgcttg
     1021 atgagatagc cataagcagt atctacgaca tccgtacttg ttagaccata atggacccat
     1081 tttctttctt ctcccaatgt ttcagaaacc gctcgtgtaa acgcaacgac atcatggcgt
     1141 gttaattttt cgatttccaa gatccgatcg atatcaaatg atgcatgctc acgaattttt
     1201 ttcacatctt cttgcgggat ttcaccaagt tctgcccaag cttcatctgc taaaatctcg
     1261 acttcgagcc acgcacggta tttgttctct tccgtccaga tattccccat ttcagggcgt
     1321 gtataacgtt ctaacatgtt ttttctcctc taatcccaaa tattcgtttg tttgatttct
     1381 tccagcgttt gctggatgtc ttctgtcaag atagtgatat gtcccatctt gcgacctttt
     1441 tttgcttgtt tttttccata gaaatggaat tgccagtctg gcttattctc aatcaagcga
     1501 tagctttcca gcagttgttc tcccaaaaca ttgaccatta ctgcattgct tatcaaacgt
     1561 actttttctc ctaatggcca accacaaata cctcgaatat gggcatcgaa ctgactcata
     1621 gaacaagctt caatagaata atgtccagaa ttgtgaggac gaggagctaa ttcattcaca
     1681 tatagcccgc ctgtttttgt taaaaacatt tctaccccta aaacacctga aaggctcact
     1741 gcttctgcaa tcacacgggc aatacgctca gcctcctcga tcacatcctg atcaatcgcg
     1801 gctggagcaa tcgtttcatg aagaatattg ttgtggtgga tgttttccac gaccggaaaa
     1861 gtcgtgaaat ctccttgacc atttcctgcc accatgatcg aaatctcttt ttcaaacggg
     1921 atccatgctt ctaaaacaca ggttccttct cttaaaaggt ccatggcagg agctagatcc
     1981 gcccgacttt tcaaaacata ctgccccttg ccatcatagc cgcctcgagt ggttttcaaa
     2041 acacaaggat agccgatccc gtcaatcgca tcttggatat ccgtcggact gacgatcgtt
     2101 gcataaggag caatcacgat gttattcgtt tccaaaaaag atttttctaa caggcggtct
     2161 tgcgtaatcg caagcaagtc tgtcccttga ggaataaatg acatgggtaa aatcgcattt
     2221 aacgcctcta cactgacgtt ttcgaattca taggtaatga catctgttct tctggcaagc
     2281 tctttcagcg caaaggtgtc atcatagaca ctttcaatgt gccaatctgc aacttgtgcg
     2341 gctggacaat cgctcacagg atccagcaca cctactttaa agcccatttc ttttgcacta
     2401 atcgccatca tacgacctaa ttgaccgcca ccgacgatcc cgatcgttga accaggcaac
     2461 aaaggtttaa ccaagttgat cactactttc tataacagtt ttagccaaca ttgaacgtct
     2521 ttcagccaat ttagcctcca attctaaatc atacatggaa agcatctgta tcgcaagtag
     2581 tccagcatta attgctcccg cttttccgat tgctgtagta gctacaggta ctcctccagg
     2641 catttggaca atcgaaagaa gcgaatccaa accatttaat gttcgtgatt ggaccggtac
     2701 accgataacc ggcaaagttg tttttgatgc gaccataccc ggcagatgcg cggctccccc
     2761 tgctcctgca ataatgattt tgattccttt ttttcgagcg ttttgtgcat attcaaacat
     2821 caaatctggg gttcgatgag cagaaacgac ctttttttca taagacacac cgaattcatc
     2881 caatatttga catgcttctt tcattgtttc ccaatctgaa gtactcccca taatcacaga
     2941 gatttttgct gtcattgttt ttcctcccag tttttctaca ttcatatttt aacaatcact
     3001 ttttttgatg tcaaagaaat ttcgaatgtt aacaaagaaa aacattttat tgttcgtgtt
     3061 tattaaagat aaaaaaaagg aatgtgacaa aagttaggtc acattccttt ttatggataa
     3121 acggtgttct cctgccaact cctcggtcac aaggaacaat attcaacaaa catgagaagc
     3181 ttttttttga aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttcct
     3241 tcaacggtgt tcaatcagct gaccctcggt attagctcaa acaaacggca aaatacgagg
     3301 aactattttt ccgtttgttt ctctaacaat caggtcaaaa cgctgatttc acaacctttc
     3361 ttttgctcta ttacttcttg aaggcttctt ccaatatttc ttcatctttt ttcaatccat
     3421 taaagatcaa attcaatatg atagccgtaa tgctactcat tacgatgcca tttccagtga
     3481 acatttgcaa tgtttgcggc atttgactga ataatgtcgg cgtaacattg aaacccagcc
     3541 caaatccaac agaaacagca acaatcaata gatttttatc attgctgaaa tcaacttcca
     3601 acagcatacg gattccttgg actgccacca tcccgaacat gactaacatt cctccaccaa
     3661 gtaccggctc agggatgatt tgtgctaacg caccgatttt agggaataat cctaaaacaa
     3721 tcaagaagaa tgcagagaaa tagatgggtt ttcttgtttt gattcccgaa agttgaacca
     3781 atccgacatt ttgagaaaag ccagtataag ggaatgtatt gaaaataccg cctaagataa
     3841 cggcaagtcc ttctgcacga tatccctttt tcaattcttc ttctccaaca tttttcccag
     3901 taatgtcacc aagtgcaaaa tatacacctg ttgattctac catgctgacc atggaaatga
     3961 tgatcattag taaaatcgac catacgtcga aagttggttt accaaaataa aagggttgtg
     4021 gaacatggaa caacggcgca tctccaatcg cgctcagatc aatcattccc atgaaagcag
     4081 ctaagatgct tcctccgact aaaccaatca ataccgcgat tgaacgaatg aacccttttg
     4141 ccagcatttg cacgcctaca attaggagaa tcgtcacaaa tgcaagtagc agatttgttt
     4201 tgtcaccaaa atcagctgct gttgcattac ctccacccat tttctcgatt gctacaggaa
     4261 tcaaagtcaa accaataacg gtgataaccg ttcctgttac tataggaggg aatagttttt
     4321 taattttaga aaagacacca gaaactaaca caacaaagat cccagaagcg ataatagatc
     4381 cgtagatagc tcctactcct tcattggttc cgatcaagat cagtggagct actgcttgga
     4441 tcgcacatcc taaaacgaca ggcaagccaa ttccgaaaaa tcggttaact gtcaattgca
     4501 ataatgtcgc taatccacac ataaagatat caattgaaat caaataagtc atctgttctt
     4561 gattaaagcc tagtcctgta ccgatcaaaa gaggcaccgc tactgctcct gcatacatcg
     4621 ctagcagatg ctgcagccct aaaaccgcag cttttccatt ttgtgtttct ttttccacga
     4681 ttatgcgtcc tcctcaagaa actcaacttg tccattcgtc aaggaagcaa tacgcgcaag
     4741 agaaacgact ctcaatccca tttcttcaag taactgacgt ccatcttgga aagacttttc
     4801 gatgacgatc ccgattcctt ctacttgcgc tccagcttgc tggcataatt cgatcaatcc
     4861 ttttgccgct tgtccgtttg ccaagaaatc gtcgataatc aatactttgt cttctgaaga
     4921 taaaaattta cgagaaatag aaatcgtact tgtcacttgt ttcgtaaaag aataaacaga
     4981 agctgtcaat aattcttcat ccatcgtcaa actttttgct tttctagcga agatcattgg
     5041 aacatctaat tctcttgcag caaataaagc tggtgcgatc ccagaagctt caatggtcac
     5101 aactttcgtg attccttgtt ctttaaatac ttctgcaaac cgttttccca tttgttccat
     5161 caatacaggg tctacttggt gagtgacgaa gctgtcgact tttaatacgc cctcgcctaa
     5221 tatacgtccg tctttttgaa tacgttcaac taattctttc actttatttg ctcctttatc
     5281 atttattaat accgaaataa ccgcttttgt gcaaaaacct actatgtttc tgttaaagaa
     5341 aaacaccctc cactttatca taagaaaaga agggtgaaat agccaatctt ttacttatag
     5401 tccagcattt acggtactgg ctagaaactg tcatccatat tatgacgata tataagtagg
     5461 tatgtaaaac taataatagt gtaaattcat tttcttgcaa ctgttttgca cgtgaaggca
     5521 gtttgccata tttgaataaa aaatgttgtt tcaaatattg aaaacagtct aacactactt
     5581 tctgaaactg acaacgtggt ttacttattt ttttgtaaaa tctaatgatt tttaatgcat
     5641 tattcctttt tgttcgggtt taaatagtta tttttatttt ctcctatcta aaaaagcagc
     5701 ccacataaaa agaaggcatg acaaaaagca tgtcatagcc cctttttccg aataaatagt
     5761 gttcaaaaga cagcctatcg gcaataaggc aagattttat aaactacggg aagtaatttc
     5821 cgtaaatcta ttcttattct tttaagctga caagctcgtc aatcactact gcttttcctg
     5881 aagcaataga atgatttgct gcgattcctg tcaaaatcga caaaatacca tctacgtgag
     5941 ttgctgcccg attccacgga tcttcttctt tttcttcacc aaatagatca tctaataaac
     6001 gcggatctcc tccgccatgt ccgcccgctt cttgttcgac gattacctca tatggctcac
     6061 cgaacatcgg atagacatgg atggactgaa gaacggctgc tccttcatcc tttttatctc
     6121 cacctgcatt gatgtaagac ttttcgatca ctttcatttc gatccgtcct ttgctgccat
     6181 tgaaagaaac attgaatcct tcccacggca tatacgcgtt tagcgaatac gtcaaaatcg
     6241 caccagtttc atatttgacc gttacgccta gtgtatcttc aatagagata tcatcggaaa
     6301 atacactgcg gtcacgaata tatccactgt ctttttctgc ttctaaatac atttctcgca
     6361 attcctcatt ggctgacagg tctatagcaa atggatcagc tttagctagt tcgcttccat
     6421 agcatcgact gtaaaatatt tctactccgc gtttctccgc attatgcatc ccataaaagt
     6481 ttaatccacc gaatgcaaat accttttggg gacggctgtt caaccaaaaa ttcactagat
     6541 caaaatgatg ggtagattta tgaacaagaa gcccgccgct attgtgctta tttcgatgcc
     6601 atcttctgaa ataatctgcc ccgtgttgcg tatttaacag ccattcgaag tggatagaaa
     6661 acacatcacc aatcacatca ctttctatca gttcctttat cttcgtgtga tacggtgcat
     6721 aacgataatt aaaagtgact cgcaggcttc ttcccgtctg tttttggacc gttattattt
     6781 cttgagcttt ttcctcatcg atagtcagag gtttttcgca aataacatca tatccttttt
     6841 tcattgcacg aatgatataa tcatgatgcg ttcgatctac tgacgtaata atgacttcag
     6901 ccggccgtac gtcatccagt agttgctcaa attgtgatgg catacatgtc tttacttttt
     6961 tctgtccata cttgttcaca agtatccgat ttgcataatc catccttgtt tgagatacat
     7021 cacaaaaacc gactagttca caagtttgat gatacgtaga ggcaattgcc tcataaaaaa
     7081 aacgcgctct cccaccaagt ccgacttgta catatttttt cttcatttaa agtcattctc
     7141 cttttctttt aagcaaaata ctgtgacaaa tggcagccct ggatacagta atgcagtgag
     7201 taaaaaatca tgctgtatac accagaacag tccatacaaa aggcataaat accacagaaa
     7261 ccccttgacg ttttttgaca acagccagat attccttttg atcaaataaa caagactatc
     7321 tgctttatct tctttgcaga tatcaattag cgtactaaaa aaactatggg atacaaaaag
     7381 caacagacac aatagaaaag gaatcagata ccaagctcca atctttccca ctaataaata
     7441 ccaatcaact gcaaaaataa tgacaaacag ctgaaacaac gtaatgatga aacacatccg
     7501 tttgattttt cctttcctcc aacatacctt tttttctgcc aagtaatgga cagatagata
     7561 ataagaagga tacatcatca aaaaataaat cagatagaca acacgagcag acagcactaa
     7621 tcctaaagtg aacaaacatc ctagaactaa agcgaaaaga gcattactga taagactggt
     7681 taaaaaaatc cgacagacat agtagttgag cttgtaaaga aacatctctt ctattggctt
     7741 tttcatattc agcccttcag accgcttgta ctgatcccct ggacaagata tttattgaag
     7801 aataagaaaa tcagaaaaac cggtagcagc gacaacgtag acatcgcaaa catactcgcg
     7861 taagtcgtac tagatccagc gtctgcaaat aatttgatgg caatcgatac cgtgtaagat
     7921 ctcggcttgt ttaaataaat caatggtccc atgaagtcgt cccatttcca ataaaactgg
     7981 atgatgatcg tcgtgatgat cgaaggtttc agcaaaggaa aaataatttt ggtaaagaca
     8041 ctatacttgc tgcatccgtc aataatcgct gattcatcta actctttggg gatgctgacc
     8101 atgaattgca tgatctggta aatgaaaaat gcttgtccaa aaaaatgtgg taaaatcagc
     8161 ggcacatacg tcccaaccaa attcagccga ttgtaaataa taaactgcgg aatcaaaatc
     8221 acttgtgcag gaatcatcat cgttactaac atgacgacga accagaatct ttttcctttg
     8281 aaatcgattc tcgataatgc gtaagcaata caagtcgagc tcagaactgt ccctatcgtc
     8341 gaaactgcag agatgacaaa cgaattccta aagaaagtaa aaaatgaaat tccagcaaac
     8401 cccgcccatc cttttgcgta attttcccat tgaaaagtct ctggaatcaa attcagcgaa
     8461 ccactcataa tctcttgatc cgttttaaaa gaacctgcga tcatccataa gaccggataa
     8521 atcatgacta gacctaatag caaaataaaa ccgtattgaa accatttcgt tactgtatta
     8581 ttcatttctt cctcctctca ttcacacaac gacccatttt tttgatgatt tgaaaatcag
     8641 aaaggtgaca gctcccataa tcaacagcat gatccacgaa atagcactag cataccccat
     8701 agaattaaaa gaaaaagctt ggttatacac ataaagagca taaaagttcg tggcatcatt
     8761 cggaccacct tgggtaatga taaatgcctg cgtaaaagtc ataaacgccg cgattgtctg
     8821 cataaccaag ttatacaaaa tgatcggcga aagaagaggt aacgtgatct tgtaaaacgt
     8881 ctgccatttg ttcgctccat cgatctctgc cgcttcataa tacgagcctg ggatctcttt
     8941 gatacctgca gcaaagatga tcattgccga accgaattgc caagcagaca tcaaaacaag
     9001 agggatcatg accagttttt gatcgctaaa ccaggaaagc gcaggtgcgc ctaatcgttc
     9061 caggaaaata ttcactaacc cgtttttaga aaacaactcc cgccaaacta gagcgataga
     9121 aatactgcca ccgatcaaac taggcacata atacaaagaa cgaaagaaag tcgttccttt
     9181 gatattcctc gttaaaagat aagcaatcaa tagaccaaag atcagtttca caggtacaga
     9241 tgcaaataca tagagaaaag tgactcggat ggagttcaaa aaacgggcat cactcagcat
     9301 ctttatataa ttatccaagc cgatccatga gactggattt cccaaacgat aatcggtgaa
     9361 cgaataataa agtgacaaga tcatcggtat cactgttatc aatagaaatc ctaggatgaa
     9421 tggagaagca aatacgtaac cgaccacatt gtcttgatcc aagaaccgtt ttagtttttc
     9481 ttttttcatg ccttcctcct gctgctatcc tagaatgttt gacgcaaact cataaagact
     9541 ctctgctgct tcttctgctg ttacttcttc ataaatgact ttttcaacta aaagattgta
     9601 ttggtcttga atttccggat tcaaagcact atcaaagaca ttcacttcgc cgttgtccat
     9661 atcggaaatc atttctaaat aatcaaatgt cgcttgcaaa gttgcatcat ctgcacgagc
     9721 gagctgttct cgaatattcg acatgattgg tacaccacgt tctcctttta aaatttcatt
     9781 ggcttcttca ctgttttgaa agaaatcaat aaacttagca gcttcttctt tatgggctga
     9841 gctttttgcc atactcagca tttgcgaaga acgtaaaggg ataccagatt ctccatctgc
     9901 ttttctgcga ggtggattaa tcaactgaat ctccatatct tcaggagccc ctgacaatat
     9961 ttctgtcaac tggttacttg atagaaacac cattgccgct tctctatccc taaccgcctg
    10021 aaaagacaga tccgtcgtac ttgcagctcc accacctggt tctggatggg cccctttgtc
    10081 taccagattt ttacgcagct ggaaaaaatc tgttagatag tctggatttt caaatcccaa
    10141 gctgttagat agatcttccc gattataaaa attcaatcct ttctcaactt gtggtatttg
    10201 aataatacag ccagctataa aatcttcgaa tcttgaaata gcatagattc ctaattcttc
    10261 agtgatttga tagctgattt gttcaaattc atcccatgtc caatttgaag ctggttcagg
    10321 gatatttaat tgttcaaata tatttttatt gtaagcaatc gcaaacgaat tcaccccgtt
    10381 agacagtcca actaattgcc cgtcgtaagt cgtcgttgct aaataggatt catctgcatt
    10441 agaaatatca attatccctt gctccacata ttcatctaat ggttcaatct gattgatgta
    10501 ttgaggaaat tgattgttca gttggaaaat gtcccagata tcatctgatg ctgctagtgt
    10561 acctaatttt gtgtaatatc cttcataatc ataaaattca taatcgatcg tcacatgggg
    10621 atttttttct tcataaagct cgattacttt tactgttgca tcgtgtcgga cttgcgaacc
    10681 ccaccaagcc atgttcagcg tgattttttt gtcatcttct tgcccacaag ccgtcaagaa
    10741 gaataacata acacttaaag ctaagatgat tctcattctt ttcttcattc tctgcacctc
    10801 tacttccatt gttatttttc aatatactgt atttttatac agaacatatt tattaaacga
    10861 tctttttcaa ggggaataaa aaccaagact tgccttatcg ttctgcctag gatgaacaac
    10921 tcaatacttt caagaaaaat tagatagtgc tgattcttct ttcatcgcta aaaacacttc
    10981 ttgcttcaat gatgggagga taattacgca aagaaaaaag gatatcagac aactgcccaa
    11041 tatccttgac atagctaaat aagcatgctt cttcgtgtgc gctcttccct aaaaaacaga
    11101 aaaataacta gtaattctgt atgaaattag gtacaatata cacgtcgatg cacaagcatt
    11161 gactatggca acttgattgg tcagttcccg ctgacttttc aagccccgac aatttagctg
    11221 ctactaaatt gtcgggtgcc ttttttagtt tttattcgtt tttcattcta tagaaagcgc
    11281 ttcaataaat caagacattt ttttgttaga atgctaataa taacaaaagg ttttaactct
    11341 tcttatgtac attacaggta aaaaaacaaa aaaatttctc ttttttttga attctgaaca
    11401 aatagtactg gagtacaaaa gacgaggaag agccactatc tgctcttcct cgtcttttga
    11461 ttctcacctg ttattcatga ttctttttct cagtttcccg atatttttcc attttacgac
    11521 taaaaagctc tccgctactt ggtggcgtat atgtgatggc atttgctcct gcttgaatgg
    11581 tctcagcaat actttcttcc gttggaccac ctgtcgcaat gataggaagt tcaggatatt
    11641 ttttacgaaa atggcgtact gcttctgcag tttctttacc tgcactgata ttgataatcg
    11701 aaataccagc tgctaacttt tcatctattt ctgtatattt cgacgttacc gtactgataa
    11761 ctggaatatc aactactcga caaacttctt ttactgtctc taaaggcgta ggaccgttca
    11821 aaacgacccc taacgaacct tgcgcttctg cgaacatgct catgtaggaa gaacgagcac
    11881 cttgtgtcaa accaccacct acaccggaga aaactggaat gtcagctgcc tcaataatgc
    11941 ttttagtaat cgctggatga ggtgtaaaag gatacacagc aattactgca ttcgcatttg
    12001 tattacgtat gatcgcaata tccgtagtaa agatgatcga acggatcaat tttccaaata
    12061 tcttgatccc gcttgcttct aaaatgattt ccggtactct tacgatatct tttctcaatt
    12121 ccgtcatgat ttctggcgta aattgttctg ccatcagcca cttctccttt gctatctccg
    12181 gtcatactga ttcaattgat acgtctcgat gacctggatg attttttgcg cataaccggg
    12241 gtctgtcgca taacctgcat cttgcaatgc ttgtgcggct tcctgatagt tggtggccgt
    12301 aatcactttt tcatacagcg caggattcca atctactcct tggacgaaca gttgtgtatg
    12361 gtcatccatg gactgttccc atgaatcata cactttgaag tctccttgga tagttatcca
    12421 ttcttcgtta acaaattcct ttgtttccag gcttactttc ttctgatccc cataagcctt
    12481 gatcccgaaa agattattgt atttgctagc caaggtactt tttccccaat ttgattctaa
    12541 tatagcttgt cctaaaatga tactaggaag cactccataa ccatcttgta gttcttgggc
    12601 atgcggaaca agccgttcaa taaattgctg atgggattgc gcttcatttt cttgctgatt
    12661 atcagtaaaa ggagaagaca gattcttcaa ggaaaaaaca aaggctaaga gtatcactgc
    12721 tacaccggca accactgctg gccaacggat attcttgcgc ttttgctttt tccacttttt
    12781 tttcgccatg atcaattcct acttttcatt taattcattc agatattctt ctagcgtcaa
    12841 ttggtgtttg gtgatatact cagcgttttc tttaccgaca taacgtaagt gccaaggttc
    12901 atatgtgatc ttagttatat cttcgtgtcc ttttggataa cgaacgataa atccatactg
    12961 cgcagcatta tctgcgatcc atttagctcc aggctgatct ccgtaacttg catccaatac
    13021 agtagaagga taactgttgt accagttttg atcgacgaca tcgactgcca gacctgtgtg
    13081 gtgttcgctg tatcctggct ccgtaatcgt ttctttagta atctttgtcg cctcctcttc
    13141 tgatacacct tgacgactca tcacatcttg gacattttgt tcgaaaactt gttgttggga
    13201 tgatacagac cggtaagctg agaccatcac taatggaaat ccagctgctt cagcagcctt
    13261 ggcaaatgct tcatattctg gagcaatgcg tttgtcgatc aagtatccgt tacttaatga
    13321 tgccaattga gtagactcat caatttcttt ttctaatttg tgatctggac caaccaaaac
    13381 tagcttccaa tcgtctgttt taacatctgg caattgtttg gacgtacgat cttttgtttc
    13441 tttgtccttt accttgctgc tggttgtttg actgttcttt tctttactag cagttgtttt
    13501 ggtgcttgtt cgttcgactg cttgattgcc ggcttgaaca tgcgaatcat catcgctaaa
    13561 aataacatag cccattgctg cgatcataat cacaactgct ccaaatccta aaaatttatt
    13621 cacttgaaaa atcctttcgt tgttcttcac gttccccatt cgcctcatga acgtaagttt
    13681 cttccagatt ttcattcacc caatcgagaa gttcgatttt ttctccttcg atctgttttt
    13741 gaacatctgc tggcagacga aaattcaaaa tatcatcata gccccattca tcatattgaa
    13801 tacttacttt caagtcaagg tatctaccag cctgctgatt atttttcaaa gggatcagct
    13861 cgacttttgc tgctttgctg ctaagccatt tttgcgcgat gcgtgttttg atttgcttgt
    13921 actctgcaac ggcaacgttt cgtttttcgg gaaaaataat cgtttgacgc aaaatctttt
    13981 gcgttagcat ccattcataa tcagtgaaaa gattttttac tttctgcatc gcttcagatg
    14041 gcagattttt ctcgccatct ttccattttt gccattgttc tggactgatc agcaagtatt
    14101 catctaaaaa atttttttca gtttcaaact gttctaataa tacactcata ataagatcaa
    14161 tatatacttg atgcacgttt atcccctcct tttaactatt ctacttaagt tttgatcaaa
    14221 aagcacctct taaatcgctg ctttcatttt ttttaatgtt tcaagtcctc tcctctttat
    14281 tcttcgtgat ttttgttata ataatagaca gacaagaaac tcaatccaac tatgaaggag
    14341 cgttttcaga tgaaaaaata tcaatgggga atcgttggtc ttggtaccat cgcacatgag
    14401 tttgccgaaa gcttcaatca agagacgagc gaactaacag cagttgcttc cagaacttca
    14461 gaaaaagcgg aaaacttcgc tcaccgttac aatattccaa aagcttacgg aagctatcaa
    14521 gaaatgttag acgatgctga aatcgacatc gtgtacattg ctgtaccaaa tagacagcat
    14581 atcgaccata tcttggcagc attggaagca ggcaaacacg ttctatgtga aaaagccatc
    14641 acgatgaata aaaaagaact ggctgacgct atgcgcttag cagaagaaaa gaatttgatc
    14701 ttagcagaag cgatgacaat cttcaacatg ccactttatc agcagttacg ctcaataatg
    14761 gatacaggaa aactgggggc tttaaaaatg atccaagcac ctttcggcag ttacaaagaa
    14821 ccagacccta aaaaccggtt cttcaatcca gaattagctg gaggagctct gttggatatc
    14881 ggtacgtatg ctgtatcttt cgctcgtttc tttttgagct ctcaacctga agtagttgcc
    14941 tctacaatgg ttccttttga aacaggcgtg gacgaacaat ccgtcactat cttgcgtaac
    15001 aaagaaaatg aacttgctgc tgtcagcttg acttttcagg caaaaatgcc aaaagtcggt
    15061 atcgttgcct ttgagaacgg ttatgtgacg attgctgatt acccgcgtgc tgatcgtgcg
    15121 gagatcttat ttacagatgg tacaaaggaa ttcatcgaaa gcgggaatac atctgaagca
    15181 atgaattacg aaataaagaa tatggtacac atgattgaag gtgacttgcc caatcgctct
    15241 ctcttcttga caaaagatgt catcgaaatc ttggatcaaa tgcaagttct atggaaaaat
    15301 atgtaatttt aaaaggttgt gaaaaagctg ttctgcatca agtaataaga acagccaaac
    15361 aaaaatagct cctcctattt ttcgtttggc tggacttatt accgcagagg aaagcttttg
    15421 aacaccgttt aagaaagagg ttgtgagcct accgttcagc tccgagtcac aaggagcaat
    15481 ttcaaaaaac agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttgacag
    15541 gagaacaccg tttaaagaag aggttgtgaa atcagctttt tgacctgaat attagctgaa
    15601 ttcacaacct ctatttttgg attttttgtt gggagaattc tgccttgatt tcaataaaac
    15661 ttttaaaaga ctgcaaaaac tggaacaatt ctgctcgatc ctcgaattca gggcgactag
    15721 tcggcagagg catttgccga tacaatacat aaacttcttc aatacgtgtt aaaatttgtc
    15781 tgccatcatt tgactctgaa aatgtttctg cagtataaat caataaacca tagatatgtt
    15841 ttccataata agctggttgc tggattctct ctagattttc cagcatgtct ttaatcacat
    15901 tggcctgtgc tcttctcata gagaagtaag tttcgtagta tagcggctgt ctgagccagt
    15961 agttttcttg atgttctctg gcatcttctt ggctttcgcg aatgaatgtc agtaaatgtt
    16021 cgcatttgat ctgcacatct tctttatttt ctgataaaag aaattctgcc atttgtctca
    16081 aaatatttcg aaattctttt tcgatctgta tttggttttc cttcagctgc ttcgtacgat
    16141 ctggcatgta aacatttgca agcaaagcaa atactaatcc aatcgccatt aatcccgctt
    16201 cattgccgat cagctgccaa gacatgtttt cctccactaa ataatgagtg accaaaaccg
    16261 aatttactac tatcccttcg gttaatttga agcgagaggc aagcgggatg aacaggaata
    16321 aaaaaagact gaagccaaaa atagtatacc caacagcaaa aaaactgaac agtgctgttg
    16381 ccgtagctaa tataaaggct gcgattcgat tcatgcccgt acgcaatgta gagcgcttgg
    16441 tatttcctac acttaataat gcaatgatcc ctgcagctgg ccaatacaac agcgataagc
    16501 tacccgctgt gaccatcgaa aagaagacac tgatcactgt tttgactgtt cgcattccta
    16561 tacgcattta cttccccctt ttttatttta attatacttg aataatgaaa aactagctat
    16621 cagacaaaag tttaggtatg atagtaaaaa gaccaaaaag gagaatctct atgcaatttg
    16681 gccgttttcg actaggtatg cgtacgatca aaagtgcctt agctgttttt ttatgtatct
    16741 tgttttttca tttgactcaa cgaggaatcc cgatgattgc tgcattatct gctgtttttt
    16801 ctttgcgtca ggacttgact acaaccgttt cttttggaaa gtctaggatc atcggcaata
    16861 cattaggtgg tacattagct atcatttatt tttttgtcaa aagctatttt cacaatgatt
    16921 ttttggtaga attgatcttg cttcctattc tagttattat tgtgatcgtc gtgtcagatg
    16981 gtattaacaa taattccggt atcatttccg caatagctac gttgttgctg attgcgttga
    17041 gcatccctca aggcgaatcc gctttgtatg ctatccaacg tgtactagat acttttatag
    17101 ggacattcat tgcaatcggt atcaatttct ttcttcgtcc tcctgaaaca gaaaagaagg
    17161 aagaactatc agaagatttg gtcgaactcc aaaagaaaga acgattgcta agaacaaatt
    17221 tacaagagat agaagctaag atccaaaaac aaaagaaaaa tagcgaggac taattttctt
    17281 ttgcaataaa ataaaaaaat ggagagaaga actttaaaaa gtcttcctcc atttttgatt
    17341 gaacggtgaa gtgtactact ttattcttcc ttcatcgagt tttgttctgc ctcttttggc
    17401 gcttcataca tatttacttg gctagtagtc ccacgtgtcg acaaattctc tacgatttcc
    17461 ttcaagtcaa gtcctgttgt cgcttttagt gtttcttgag tagaagaaag caaatttgtg
    17521 gcataatttg tcactcggtt cgctccactg gtttctcctg aactgcttcc tgtatcgaca
    17581 acagagattt tttcgatatt tcctagcggc tgtgctgctt cgcgcatcaa ttgcggtagc
    17641 atatcgataa ccatactcaa gacagctgct tcgccatatt ccttgaaggc attggcgatc
    17701 ttttgttttg cttcggcttc tgcttttcct tttgctagtg ctgcttcggc ttctgcttgt
    17761 ccagctaaac gtgtcttatt ggcttccgct tctgccagtg cttccacacg gaatcgttct
    17821 gcttcggctt cagtcacttc tttgactttt tgagcttgtg cttcttgttc tttagcgtac
    17881 cgatcggcat ctgctttttt cttcacttcc gaatcatatt gcttttcacg acgtgtgatt
    17941 tctttttctt ccagttcgat ttgtttttga cgttctacga ttttaatctg catttcctgt
    18001 tcgattactt gttgttgtgc tcgggcactt tctaaattgt aggcttggtc tgcttttgct
    18061 ttagcaacat cttgttcttg tttgtaggtt gctaatttca gttctttttc ttttaatgat
    18121 tctgcgatct ctgtttgtcg ctgtaattca gctgcttgtg attctttttc tgcttgagct
    18181 tttttgatac gcgtttcttt tagagcttcc gcttctgcta tatctgcatc tcttttgact
    18241 tgggcgattc ttggtttccc taaagagtca agataaccgt ttttgtctct gacctctttg
    18301 atagtgaaag aaacgatgat caaccccatt tttgcaaggt ctacagaagc gacttcttgt
    18361 acgctttgac tgaacttatc gcgattttga tagatttctt ctacagtcat agaaccaaga
    18421 atcgagcgca agtgtccttc taatacttct cttgcttcat tttccagttc ttctcttgtc
    18481 ttgcctaaaa actgttctgc tgctgtagca atctcttcta cagatgaacc gattttgatg
    18541 atcgaagtac catcacacat caccggtacg ccttgttccg tatacacttc aggggtggag
    18601 acgtccaatt tactcgataa tagactgatc cgattcgaac gttgaaaaac tggcaagacg
    18661 aatgcgccac ctccacgcac aattttgatt ttgttattgc tttcatcaat gtgcacattt
    18721 ttgcttccta aataacttcc actgatgatc aacgcttcat ccggttttgc tgtctggtac
    18781 ttcgctacaa agacgatcag caacatcagt aaaatcaaag ctgcgattgc gatcgcgata
    18841 aaaacaactg gtatttccat ttaacacacg ctcctttaag cttttatcat gctttcataa
    18901 ggcatgacat aacagattct ttccttgatc tcaatgatca atactttcgt tccccgctca
    18961 gctgtctgtt cttgggggct gtaaaaacaa gctggacgac taatggaacc ggtaacactt
    19021 ttcaactgaa tttctcccat acctcgtaca gggatcggtg tgatcacaac agctacttgt
    19081 ccttccagag ttttttcaga agacgacatc gttgattcgg catgtttcat cggcatcaat
    19141 atatagaaat taagcaaaaa taccaacact atcgatatgc ctccactagc aataaataaa
    19201 aataaagatg attgatctgt taaacgctca cctagaaaac caaataagct tgtgaaagtt
    19261 aaccaaggga taataagcat cggatcaagc gggccatcaa aatcgaatac atctccaaaa
    19321 ataatcaata ggactgccaa tcctgcacaa atcagcaatg tataccaata aatcgtctca
    19381 agtgcgattc cccctatcat atacacgcct ccttccattt ctacgtctat cataccaatg
    19441 tttgaaaatt ctgtatagct tcttttaccg actcagtggt gttttttata ggaaggttgt
    19501 gtttatcgtt gacaatttgt taaatttttg ttttcatgtt aaaattttca caataaattc
    19561 tttcgaggta gcctatgttt ccttttttta agaaaaaacc attaaagctc cagaacacga
    19621 tcatccttgt cgtttgtttc tccacattcg tttctttgat tgtttctgcc atattgatca
    19681 ggaattttat tctttcgatg aaccgatcta gtgtgtcaga taaaatctct agcattgcta
    19741 aaatcgttgc caatgataaa ctcatccaaa aaaagctgca gactggtgac ccgacacatg
    19801 cgattcaagg aataacaaat gaaatcatga ttgcatcgaa tttagatttt atcgttgtca
    19861 tggaccctga aggcattcga tattctcacg cagacccaag ttacatcggt cttcctttct
    19921 caagtcatga agatgtgaaa aaagtgattg gaggacaaga atttttctct gaaaaagaag
    19981 gaatgttagg gttcggcttc cgctatttcg tccctattta ttatgatcaa gagattattg
    20041 gtgcagtttg tgttggctta acaaacaaca cgattgacaa tcaagtcaaa gatggacaaa
    20101 aaatgattgt tttttcattg attattggac ttagcgtagg catcataggc gccgtttttc
    20161 ttgcccaaaa aatgaaaaaa gtactgcttg gacttgaacc acaagaaata gcagaacagt
    20221 taagtgaaaa aacgattatc gaaaatgaag tagatgaagg aatcttagcc atctcttctg
    20281 ataaaactat cctcttgatc aatacacctg caaaaaacat tttctaccaa acagagcagc
    20341 tattggattt cgaagtagga aaaaaatcag tgataatcta tatgacgttt tgtttaaaga
    20401 gacgattgag catcaaacga ccgttcggac tcaatccatg tatttaaatg ggatagaaac
    20461 tatttctagc gttacacctc tttttagcaa tgaccgtttt ttaggagcag tcgcaacttt
    20521 acgaaatcga tcagaagtaa tgaaattaat ggaggagctt ggggggacga aacaatatat
    20581 aaactccatg agagctcaaa cccatgaatt catgaataaa cttcatgtca tttctggttt
    20641 gattgatttg aagaagtatg aagaagtcac ccattttatc cagcagctaa atcaaaatta
    20701 taaaaaagag attggccaag tctcaagtct gatcaaagta cctgcaattg ccggtttttt
    20761 attaggcaaa ataaacgaag ccaaagaaca atctgttgaa atgctgttag aaagcgattc
    20821 ttttatccct aaaatggaga tggatgactc catccattca cttttgcaag tgttagggaa
    20881 cttacttgat aatgcaaaag aagccgtgct acaaaaacat gaaagaaacg gacaggtccg
    20941 gattcattta aactatgaca cagaagctga agtattcatc gtgaccgtct gcgatgacgg
    21001 tgtcgggata tcagaagaaa tctcagaaaa aatatttacg ctaggttttt cgacgaaggg
    21061 aatcaatcgt gggtttggat tgaatctcat ccattcaatc gtcaaacgcc acggtggatt
    21121 tttagaattt tccaaacaaa caaatggcgg tacaatcgtc tatctagaat taccaaaaaa
    21181 ggaagatcaa aatgatgaca aatgtcctga taatcgaaga tgatccaatg gttgcagaat
    21241 tgaatcgtac ttttgtggaa agcatcccta atatgaacgt aatcgaaagt ttcagaaatg
    21301 tagaagaagg aaaaaaatat ttcagacaaa aaaacgcaga tctgctgctg cttgatgtat
    21361 acatgcctgg cgaaagtggt atccattttt tatcttggct tagacagcaa ggaaatgata
    21421 tcccagtgat tttgatcact gctgcagatg atatcgaaac cataaagaat gtgatccgct
    21481 acggtgcctt agattatttg atcaaaccgt tcactttgca acgttttcag ctggcgatcg
    21541 aaaagtatct tgctttctct cagacaatcc aaacgactga aaaagccgat cagaaattga
    21601 tcgatcaagt attcaaccac accactccca ttcacgaaat accatcattc atcgatttgc
    21661 ctaaaggtct gtcaaaactg actttaaaaa aaataagtgc atttattcta aaagaagata
    21721 ctattttttc aacaaaagaa ctggctcaaa aaagctcact gtcacgtata tccgtcaaga
    21781 aatatttgaa ttttttagta gaacaagata tattgaacga aagaatcaaa tatttggaaa
    21841 tcggccggcc gctaacctgt tatgaattga caaagaatgg gcggaaaaag ctcacagatt
    21901 actgttgagt catcgaatta tcccatacaa aatagtccaa gtatacagaa gtaacaactg
    21961 tttacttgga ctatttttaa acattttatt gacgaatatc catgttttct tatcttaatt
    22021 gacgggcttt aggaagagat acctcatttt tcgtttttcc agtttctatt atttcacgaa
    22081 agttctcgaa acttgtcgaa atcatatttg ataatgcctc atctgtattt gaacctacat
    22141 gcggggttac aagaactcta gggtacattt ctactaattg ctggatagca gggtctggaa
    22201 tcgtctccca tggcttgaag gatcggaaaa aaacctctgt ttcatttgca aaaacatcgg
    22261 ttgcaaaacc agctaaatga ttgcttttca aagctcttaa aatagcttca ttatcttgaa
    22321 gctctccacg ggctgtatta atcaagatgc tacctttttt catttgagct aaaaatcctt
    22381 cattgatcat ttgatcgttt tcacctggga tatgaggtac atgaatactg acaatatcgc
    22441 ttttggctaa taactcttca agcgagtgga acgtcaatac ttttttagca gcttctgtct
    22501 gatgaatatc gtagccgatc actttggctc ctagtccctt aaacaaaaca gcttccgtca
    22561 accctatcct gcctgttcct atgatcccta ctgtgcaatt tctgatttca cgactaaaca
    22621 tttgctcatc cactcgaaaa tctttgaaag atgttcggat cgtcgtatac gcagtatttc
    22681 tcaaaagcat catggcaaaa gtcaaagata actctgctat cgcatttggc gaatacgatg
    22741 gtacacgggc tactgtcata ccaaaatcag ctgccgcttg tagatcgata tgattgactc
    22801 ccacagtccg tgtaaaaaca taacgaatcc catattcctg catttttgca agattggtct
    22861 catctgctat acaatttccc ctcaacaata cagcatcatg gtcctttgct gacacgatat
    22921 tgtcatgtgt caacagttct tcataaagac tgagatcata atggtaaata ttcaattttt
    22981 caaaaaaatc tatctcgttc ggacgaacac cgtaacatgc gattctcata ctttcttcct
    23041 ccatttctta tgaaaataaa cctatgccat gaatcatttg taatacaacg atccatacag
    23101 gtgtcagtac cacagcaaaa atagttgaca gtaaggatgc attggaggct aaaagtgcct
    23161 ccctatcaaa actgatagca taggctgctg ccactgttgc agctggtgta gccatcatga
    23221 taacgattgt tgccagtgct tcaaaattga ctggtagaat attcgttgcc gtcaaaactg
    23281 ctagaatcac gatattcaat gcaggtacaa ataccacttt gactagactg taataccatg
    23341 aagttttttc agatgcagct tttttgaagc ttacttcacc taatgttgcg ccgattgcta
    23401 gccacgccaa tggtgaactt aaaccagcta aataatccat cggctgaaat agccacggtg
    23461 cagtctggtc aattcgcaaa aacgctactt ggcttatctc tccttctgca cctgtaactg
    23521 acacttgtgg cagataccct tggaacagcc aaatgaaaag gccggcaaaa gtagcgataa
    23581 tgattggatt gacgaacatc gctttgatat ttttagcttc catcttcaag ccactcattt
    23641 ttatgtatcc ataagaatac aaaaagattc ggtagccaat attgaaaatt gaggaataca
    23701 tcacgccgac aggaccataa atcgcactga cgataggcgt tccgaaaaag gtcgtagaac
    23761 caaagatcgt caggacacgc aacgttgttt gcttatcccc tttatagctt aagaaaaaag
    23821 gttttgaaac tagaataaga ataatataaa taactaatcc ccaaatcaag acattgattc
    23881 cttgcatcag taattctgta ttgatatctt gcatgaaagc tcgaaatgcc agtgctggca
    23941 aagccactgt taagacgact ttcgatagaa tttttccaac agattcagaa aaaatcccct
    24001 tttttctaca aacaaaccct aataatataa taaaaactgt tgaagtgatt gcactgacaa
    24061 tcgacatatc tgtcaaagtc gatcgaaaaa tgtcaaataa gttcatcttt ctcctcctaa
    24121 ttatgttcat ttattcacaa taaaaaaagc ttttattttg ctaatacgta ttaacatttt
    24181 taattttatc tttacattgt tacttattga acaataggaa attaaagaaa cttttgaaac
    24241 ttatgtaaag tgatt
//
LOCUS       DAYYAR010000028        24231 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000028 DAYYAR010000000
VERSION     DAYYAR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.028"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..753)
                     /locus_tag="ACHAT0_001002"
     CDS             complement(109..753)
                     /locus_tag="ACHAT0_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8450961.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(847..981)
                     /locus_tag="ACHAT0_001003"
     CDS             complement(847..981)
                     /locus_tag="ACHAT0_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8450962.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(983..3115)
                     /gene="feoB"
                     /locus_tag="ACHAT0_001004"
     CDS             complement(983..3115)
                     /gene="feoB"
                     /locus_tag="ACHAT0_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8450963.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(3112..3585)
                     /locus_tag="ACHAT0_001005"
     CDS             complement(3112..3585)
                     /locus_tag="ACHAT0_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8450964.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            3986..4210
                     /gene="nrdH"
                     /locus_tag="ACHAT0_001006"
     CDS             3986..4210
                     /gene="nrdH"
                     /locus_tag="ACHAT0_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8450965.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            4369..6528
                     /gene="nrdE"
                     /locus_tag="ACHAT0_001007"
     CDS             4369..6528
                     /gene="nrdE"
                     /locus_tag="ACHAT0_001007"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8450966.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            6578..7543
                     /gene="nrdF"
                     /locus_tag="ACHAT0_001008"
     CDS             6578..7543
                     /gene="nrdF"
                     /locus_tag="ACHAT0_001008"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8450967.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(7632..8771)
                     /locus_tag="ACHAT0_001009"
     CDS             complement(7632..8771)
                     /locus_tag="ACHAT0_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8450968.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(9080..9793)
                     /locus_tag="ACHAT0_001010"
     CDS             complement(9080..9793)
                     /locus_tag="ACHAT0_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8450969.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(10047..10748)
                     /gene="nagB"
                     /locus_tag="ACHAT0_001011"
     CDS             complement(10047..10748)
                     /gene="nagB"
                     /locus_tag="ACHAT0_001011"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8450970.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            10982..12514
                     /locus_tag="ACHAT0_001012"
     CDS             10982..12514
                     /locus_tag="ACHAT0_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8450971.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(12705..13907)
                     /locus_tag="ACHAT0_001013"
     CDS             complement(12705..13907)
                     /locus_tag="ACHAT0_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8450972.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(13989..14678)
                     /locus_tag="ACHAT0_001014"
     CDS             complement(13989..14678)
                     /locus_tag="ACHAT0_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8450973.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(14784..15152)
                     /gene="rplL"
                     /locus_tag="ACHAT0_001015"
     CDS             complement(14784..15152)
                     /gene="rplL"
                     /locus_tag="ACHAT0_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8450974.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(15205..15708)
                     /gene="rplJ"
                     /locus_tag="ACHAT0_001016"
     CDS             complement(15205..15708)
                     /gene="rplJ"
                     /locus_tag="ACHAT0_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8450975.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(15912..16039)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(16118..16807)
                     /gene="rplA"
                     /locus_tag="ACHAT0_001017"
     CDS             complement(16118..16807)
                     /gene="rplA"
                     /locus_tag="ACHAT0_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8450976.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(16911..17333)
                     /gene="rplK"
                     /locus_tag="ACHAT0_001018"
     CDS             complement(16911..17333)
                     /gene="rplK"
                     /locus_tag="ACHAT0_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8450977.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            17676..18344
                     /gene="sdaAB"
                     /locus_tag="ACHAT0_001019"
     CDS             17676..18344
                     /gene="sdaAB"
                     /locus_tag="ACHAT0_001019"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8450978.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            18357..19229
                     /gene="sdaAA"
                     /locus_tag="ACHAT0_001020"
     CDS             18357..19229
                     /gene="sdaAA"
                     /locus_tag="ACHAT0_001020"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8450979.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(19320..20420)
                     /locus_tag="ACHAT0_001021"
                     /pseudo
     CDS             complement(19320..20420)
                     /locus_tag="ACHAT0_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(20512..21399)
                     /locus_tag="ACHAT0_001022"
     CDS             complement(20512..21399)
                     /locus_tag="ACHAT0_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8450980.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(21476..22021)
                     /gene="nusG"
                     /locus_tag="ACHAT0_001023"
     CDS             complement(21476..22021)
                     /gene="nusG"
                     /locus_tag="ACHAT0_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8450981.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(22122..22292)
                     /gene="secE"
                     /locus_tag="ACHAT0_001024"
     CDS             complement(22122..22292)
                     /gene="secE"
                     /locus_tag="ACHAT0_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8450982.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(22316..22468)
                     /gene="rpmG"
                     /locus_tag="ACHAT0_001025"
     CDS             complement(22316..22468)
                     /gene="rpmG"
                     /locus_tag="ACHAT0_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8450983.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            22630..23274
                     /gene="cbpA"
                     /locus_tag="ACHAT0_001026"
     CDS             22630..23274
                     /gene="cbpA"
                     /locus_tag="ACHAT0_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8450984.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(23330..23833)
                     /locus_tag="ACHAT0_001027"
     CDS             complement(23330..23833)
                     /locus_tag="ACHAT0_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8450985.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(23846..23892)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         7431 a         4963 c         4352 g         7485 t
ORIGIN      
        1 tttgtcaaaa atgaaagaaa tttgcttgtc caaactgaaa aaaacttggc taaatctttg
       61 taaataaaag gtttagccaa gttaatattt ttttaatatt tcacacaatc attctgcaag
      121 ctgatgctcc gggtaaaaaa agtttgtcat acgctgtagt gtatattcat ttccaataaa
      181 atgaacgatg tcccctgccg taaatttagc ataggggcca ggtgaaacaa gcagttcttc
      241 attatgagaa attgcgataa ctgtggcgcc agtcgcctgc cagatattca actcgctaat
      301 tgaacggtcc agatgcattg ctttttctga taaagataat tcaaacggaa gtaaaggatt
      361 catcttatgt attcgcttcg tttgattgac cagctgatcc aataattgag aaaaatgatt
      421 cagctcatct tgttgcttct ccacacttgc catgatttct gcttttgtat cttggatgga
      481 ttgtacatct tgatattgca ataggaaatc ttttgctttt tccttcgaag caacaaatgc
      541 cccacttcca tgccgtactt ccatgattcc aagatctacc agcacattga tagccttgcg
      601 ggtagtctca ggagaagcat tgtacgtact ggcaagtgtt gagcgggcat ggatcttctc
      661 acccacttta tatcggtttt caacgatacg ttcggctaaa tcaacagcaa tctgctggta
      721 gcgcgggcgg acgatttttt tctttccaga cattttcttt cctctttctt tttttacttt
      781 ccctcatctt actatgctct agcaaaataa gcaaaaaagg ggagaatagc ctcttgcaac
      841 gactgatcat cgcttgatct gttccttttt tactgggcaa gctgtttgac agtcatcaca
      901 attcttaccg gattttaaac gagtatagac aattgcgcct gcacttccaa agattaacac
      961 acttaataag aatgtcgcca tcctattcct ctccttctaa tgacaatata gtataaaact
     1021 ggttcttgga ttttggctga cggaacaaaa cataaacgag tcctatcatc agtaaaatag
     1081 caagatacgt ccctaacgct acctgtccac cctccaataa gtgtcccaac tgataaatca
     1141 caaaactaac taaataagct aaaccgcatt gatagcctat ggcgatccaa gtccatttct
     1201 tggcattcat ttcccgatgg atagccccaa ttgccgcaaa acaaggcgca caaagtaaat
     1261 tgaaggctaa caatgaataa gctgctgcgg gagtatatgt gtgttgtagc acttgccaga
     1321 tttctctgcc attttcggat acttccgcat gaccaaacaa aataccaaat gtgccaataa
     1381 tattttcttt agcgaccaat cctgtaatcg ctgcgactgt tccttgccag tttccccaac
     1441 caagcggctg aaaaacaact gccaggattc gcccgaaaaa ggctaagata ctttggtctt
     1501 cagaaacagg ttgagcataa aaattataag aagagctgaa ccagatcacg atactagaaa
     1561 caaagataat cgttcctgct tttttaacaa aagacttgct gcggtcatag gtttgtctta
     1621 ggaccgctga ccattgagga aaatgatatg cgggcaattc catgataaaa ggagctgtat
     1681 ctccagcaaa caaacgtgtt tttttcaagc tgattccaga gaaaataatt gcagtcatac
     1741 caagaaaata ggcagaagga gctacccaag attgatgagg aaaaaaggcc cctgctacta
     1801 aggcaataat cggcaacttt gcagaacaag gcatgaaggt tgtgaccatg atcgtcaaac
     1861 gacgatcttg ctcgttttca atcgtgcgac tggccatgac gcctggcact ccacatcctg
     1921 tagcaatcag cataggaatg aaggattttc ctgaaagacc gaatttcctg aaaagtcgat
     1981 ccatgacaaa agcgatcctc gccatatatc cgcagtcttc cagaaagctc aagcataaaa
     2041 acaatataat caattgcggt agaaatccta acactgctcc cacgcctgcg atgatgccgt
     2101 tcaagatcag ctcctgcatc catcccgcta cttgccattc agctaacaat ctgccaacat
     2161 gttctggtaa ccatgtacca aacaatgtgt cattgatcca atctgtcccc attgttccta
     2221 ctgtttgaat agccagataa tagatcagcc acatcacaaa tgcgaagatc ggcaaggcta
     2281 accaacgatt cgttgcaatc tgatcgattt tgtcactcat cgataattgg aaagaatcat
     2341 ttgacacaac acagagtgta catagttggg taataaattc ataacgttca ttgaccagaa
     2401 tcgtctcact atcgtcgtcc aatagtttct ctgttaactg gatcagttgc tcgatctctt
     2461 tttgctgaga aactgctatc tcgtaatcct ctcttacctt ctgatctcgt tcaaaatatt
     2521 tcattgcata ccagcgtttc ttgccgcttt gttctcctag tacatcttca atttcatgta
     2581 aagctgcttc taaacgtgga tcatatgtac agggaacagg aatagaaggc cgcttttttg
     2641 cagactcctg aatcttttcg attagttttt cgattccttg ctttttcaat acgctgattg
     2701 gtaaaactgg tacaccaaga ccataggaaa gcttttctag attgattttt tttccttctt
     2761 ttttgatgac atccatcata ttcagaccaa tgatcatagg aatgtctaaa tccaataact
     2821 gcgtagttaa ataaagattt cgttctaaat tggtagcatc aataatgttg atgatcagtt
     2881 caggctgttc ctgaaccaaa taatctcttg caatcacttc ttctggtgta taaggagaca
     2941 aagaatagat gccgggtagg tcctgaatat gaatgttcgg attttttttc aacgatcctt
     3001 cttttttttc tactgttacg cccggccaat tccctacata ttgattcgaa ccagtcaaga
     3061 gattgaacaa gctagttttc ccgctgtttg gatttccgac taacgcgata ttcattcttt
     3121 ttcctccctt tgtatcaaga ttagttcggc ctctgacttt cgtaacgtca gttcataccc
     3181 tcgcactgtc agttcgattg gatctcccag aggggctagt tttcggataa ctatttttac
     3241 atttcgcgtc aatcccatat ccattaaccg tcgcttcact gcgccttgtc cgtggattgc
     3301 tacgacgatc ccagtctctc ctgcttttaa ttgatcaaga gaagtccagc tttcagagga
     3361 agcagtcttt ttattgacaa agatttgacc tagtatttcc cgacttactg ccactcgatg
     3421 actttttacc agtaaaatgg cattgttgcc tgataaattg acgacgacga ttttacttcc
     3481 ggcaacgatc ccgatatttt gcaaatggcg ttttgttttt tcttctgttt cgattcgttc
     3541 gacttgataa acgaaattct gctttgcctg cgctaaggtc tgcatattga ttcctctctt
     3601 gtgaaataaa tcatgtatta aaatcattca ttatatgttc taattttctt acgtgttacg
     3661 caaataaatg agaatcattc tcacatagaa tactctcggt aattgagaat gtcaattata
     3721 cttgtgatat tatgagcgaa tctttagtat tgatagataa aaatgagaaa aaaatatttc
     3781 ttcatagttt tccacaattt tttctctatt aaatttgcta ttttcccaca cgatacttgt
     3841 tttcagctct tttttcaaaa acacaaaact tagtattcca cagtttccac aacacaatat
     3901 gtagtgtttg ttgtttgact tttttactaa gagttttata ctatctatta tagataataa
     3961 gaatgtgtta gaggaggcac tcaccatgaa cgtaaaaatc ttttctaaaa ataattgtat
     4021 gcaatgtaag atggtcaaac ggtttttaac agaaaatcaa attgcttttg aagaagtcaa
     4081 tatcgatgaa cagcctgaag cagtagactg gttgaaagca caaggattcc aaagtgttcc
     4141 tgtcattact tctgatgcag aaacagttat cggattccgt cctgatcagt tgcgtaaact
     4201 tgccagctaa catgactaaa agaatcaaaa cgtgatacat ggatttgaag ggcttgagac
     4261 agcaccagtt tttgtcgcaa acttccaaat ccttttttaa agcttaaaaa caatgcaatg
     4321 atttgcaatc aagcatcatc aaatacgaaa tgaaggttgg gcgtacgtat gagtcttaaa
     4381 aatttacaag atgtcagtta tttcaagctg aataatgaaa tcaatcggcc agtcaatggt
     4441 caaatccctt tgaataagga taaagaagct ttagttgcct tttttgagga aaatgtcaaa
     4501 ccaaatacga tgacttttct atctattatg gaaaaaatca attacttgat cgatcaagaa
     4561 tatatagaag cagaattcat tcaattgtat tcaccatcat ttattgaaaa gctatatcaa
     4621 tttttggacg agcaacattt ttctttcaaa tcttttatgg ctgcttacaa attttattct
     4681 caatatgcgt tgaaaaccaa cgacggttcc gcttatctgg aaacttatga agatcgagta
     4741 gccttcaatg cgctttactt tgcgaacggg gatgaagaat tagcacttgc tcttgcagac
     4801 gaaatgatcc atcaacgata tcagccagct acaccttctt tcttgaatgc tggacgtaaa
     4861 cgtcggggcg aattggtgtc ttgtttcttg actcaagtca ctgacgatat gaactctatc
     4921 gggcgctcaa tcaactctgc tttgcaattg tcccgtattg gtggcggcgt agggattact
     4981 ttatcaaatc ttcgtgaagc cggcgctcct atcaaaggat atgagggtgc tgcaagtggt
     5041 gtcgttccag tcatgaaatt attcgaagat agtttcagct attctaatca attaggtcag
     5101 cgtcaaggag ctggcgtggt ttatttgaac gttttccatc cagacattat tgctttcctt
     5161 tcagcaaaaa aagaaaatgc agatgaaaag attcgcgtaa aaactctttc tcttggcgtg
     5221 atcgtacctg ataaatttta tgaattgaca cgtaaaaacg aagatatgta tttatttagt
     5281 ccatatagtg tcgaaaaaga atatggtgtc ccattctctt atgtaaatat tacagctgag
     5341 tatgatcatc tagttgcaaa tccaaacatt cgcaaacaaa aaatcaaagc gcgtgatctt
     5401 gaaaatgaaa tctcaaaatt acaacaagaa tctggctatc cttacgtaat caatattgat
     5461 acagcgaacc gcgagaatcc aatcgatggc aagatcatca tgagtaattt atgttcagag
     5521 attttacaag ttcaaacgcc ttctgtgatt aacggcaaac aggaatatga agtattagga
     5581 acggatatct catgtaactt aggctcgaca aatatcgtga atttgatgca aagccctaac
     5641 tttggtcaat ctgtgcgtac gatgactcgt gcattgacat ttattacaga tgcatctgat
     5701 atcgatgtcg taccaccgat ccaaaacgga aataagctga atcatacgat tggattaggt
     5761 gccatgggct tacacactta tcttgcaaaa gaacaaatgg aatatggctc agaggattca
     5821 ttagacttta caaatattta cttcatgctg ttgaattatt ggacacttgt tgaaagcaac
     5881 aatatcgctc gtgaaagaaa acaagttttc cataattttg aaaaatcagc ttatgctgac
     5941 ggcacttatt tcgaaaaata cgtaactgga gaattccagc caaaatcaga aaaagtcaaa
     6001 gcattgtttg aaggcatttt tatcccaact gctgaagatt ggaatgcttt gaaacaagca
     6061 gtaatgaaag atggactgta tcatcaaaat cgcttggcag tagcaccaaa tggctcaatt
     6121 tcatatatca acgatacgag tgcaagtatt catcctatca ctcgcatgat cgaagaacgc
     6181 caagagaaga aaatcggtaa aatttattac ccagcgcctt atctatccaa tgagacgatt
     6241 ccgtattaca cttctgctta tgatatggac atgcgtaaag taatcgatgt ttatgcaatg
     6301 gcacaacaac acgtcgatca aggcatgagt ttgactttat tcatgcgttc tgaaatccca
     6361 gaaggcattt atgaatggaa gacaacaacg aagcaaacga cacgtgattt gaatatttta
     6421 agacattacg ctttccacaa aggaatcaaa tccatttatt acgtacgtac ctttactgat
     6481 gatgcagaag aaatcggaag caatcaatgt gaaagctgcg tgatctaata tcatagatag
     6541 attcgcttat tcatatttta attaaaggag cattctcatg tcagaaacat attatgaagc
     6601 aataaactgg aatgaaattg aagatatcat tgataaatcc acttgggaga agctgacaga
     6661 gcaattttgg ctagatacac gtatcccact ttcaaatgac ctagatgatt ggcgtacatt
     6721 gtctcaattg gaaaaagata cagtaggaca tgtatttggc ggtttgacac tgctagatac
     6781 tgtccaatct gaaagtggca tggatcagtt gcgtaaagat gtccgcactc cccatgaaga
     6841 agccgttttg aacaatattc aattcatgga atccgttcat gccaaaagtt attcttctat
     6901 ttttagtaca ctgaatacga aaaaagaaat cgaagaaatt tttgactgga caaatacgaa
     6961 tccttatctg caaaaaaaag ctgagcgtat caatgaaatc tacaaacacg gtacaccgct
     7021 agaaaagaaa attgccagcg tctttttaga aacattcttg ttctattcag gtttttatac
     7081 tcctctttat tatttaggaa acaacaaatt ggctaacgta gcagagatca tcaaactaat
     7141 catccgagat gaatctgtac atggaaccta catcggttac aaattccaat taggattcaa
     7201 cgagttacct gaagcagaac aagaaacgtt aaaagactgg atgtacaatc ttctttatga
     7261 gctttacgaa aacgaagaac gttatacaga agaattatac gatccaatcg gctggacaga
     7321 agaagtcaag acattcctac gctataatgc caacaaagca ttgatgaacc tagggatgga
     7381 tccactcttt ccagatactg ccaatgatgt caacccaatc gtaatgaacg ggatttcaac
     7441 aggcacaagc aaccatgact tcttctcaca agtcggaaac ggctatttgt taggtcatgt
     7501 agaagcgatg aaagacgacg attatttgat tggattggaa taaggattta ttttaattca
     7561 aaaatttttc gaaaaataaa aagagtttag cgtacagata ttttaaatgt acactgaact
     7621 cttttttatg cttattcact tgtttcttcc agcacttttg gactgtctaa cttttccgaa
     7681 acatgttcca ttcttctcaa taaatagatt ttccggatat ttaatattat cgtcttaatc
     7741 actgcataga caggtatgca aatcagcatg cctaatattc ctgcgacact tcctgctcca
     7801 atcaatacca agatgatcgt caaaggatgg atattcattg atttcccaaa taacaacggc
     7861 ttgatcaaat ttccatctaa ttgttggatc actaaaatcg acaacgccat atacaaagct
     7921 tgaaatggag agataaacaa accgacaatg attgctggta ccgctccaat aaaagggccg
     7981 acatacggga taatattcgt gattccgcaa atgaccgcaa gcaacaagct ataaggctga
     8041 tcgaatattc tcattgccaa aaagctcatt acaccaataa tcaaagcatc taagatcgta
     8101 ctgctgatat aggcagataa tgtttcgttt aattctttta ccgtttggcg taactcttta
     8161 cgaatcgctt tcggaaagaa gtgagacaac gcatcaataa atttgtgccc atccttgaac
     8221 ataaatagca aaataaatgg aacggtaaat aacaaaataa aaaattgcat cataaatgaa
     8281 atgatttttg agacacttgc agataaactg actaatatga tattgaacaa attactaaga
     8341 gataaatttg ctgatgctaa ttgttcttcc aaattgaaat ttttaaactc ttcccgctgc
     8401 gccaattcat ttaaccattt gcttgtttca tctgcataca ttggcaagct ctgggtcaat
     8461 tgaattgtct gttccattaa ctgaggcact acattcatga cagttaaaac aactaaaatg
     8521 attacgacca aaaacgtcaa taaaaaaccg aacactctcg ggatccgatg cttttcgaga
     8581 aaaacaacga ttggatcgaa catataatag ataaaaccag caacaagcaa tggtaaaaaa
     8641 actgccgaaa gcattttcaa tattggacta aaaatattcg gcatttgtaa aataaagtac
     8701 acgccgatga tcgtgagtac tagctccact gtccagaaaa ataattttga ttgtttaaaa
     8761 cgtgattgca tacatctcct ccaaggtcct ttgatacttc tattctatca tttaacaaag
     8821 agttacgcgc tgagattttc atttgcttaa aaaaaacaaa aaaaataaac atattttaat
     8881 gagtagacga gtacttaaac aaaaacacgg tttggagtag tgacaaaaat tatactgcag
     8941 tccctttttc caaataaacg gtgttcaaaa gcttgcatct tcgtaataag cccagccaaa
     9001 cgaaaaatat aaggaactat ttttatttgg ctgggcttat tacttgatgt aggacatcta
     9061 ttttacaacc tcagatcatt tattgcgcaa tactgactaa atgcaacttt gtgatatttt
     9121 tgtgtacgga caactcctcg atcacatcgg cataagtgag cccttttgga agatcaatcg
     9181 tatagatatt tttgtacact cggtagtcat caacgaattt tacgtcaaaa ttcacatctt
     9241 cgattacgat atttttctct tcaaaataat cggtaagaaa atctttggtt tcttttctat
     9301 ggataaattg gatttctaac tttttagtcg taggtacatg gatcacgcgt ttcactactg
     9361 tgagtgcaat agcaatcccg ataaaactga tgatcgcaat ggaatagtaa cccatgccga
     9421 tggcgatccc taatccagca attgcccata aagaagcggc agtcgtaagt cccgtcaccg
     9481 attgtttcgt gacgatgatc gttcccgcgc ctaagaaacc gacaccactc accacttgag
     9541 caatcagcct tgcttcatct gaacgaatcg ttccagcaag ctgtggatac tctaaagcat
     9601 ctcttaatgc accactagcg atctcaactt ggatcaatgc aatgatacat gctcccatac
     9661 aaactaaaat atgcgtacgc atacctgctg gacgattttt atattgtcgt tcaaaaccga
     9721 ttgctccgcc aaatagcata gcgatcccta aacgtaaaat aatctctgga attgtcaata
     9781 ccatatcatt catctaaaca ccctccagtc caatatatca tgtctgaaag gagcagacaa
     9841 aaaaagtgca atttttacct cttacgaaaa aaggactgtg acaaaatttt tgtcatagtc
     9901 ctttttatga ataaacggtg ttcaatcagc tgaccctcgg tattactcaa caaacggcaa
     9961 aatatgggga gctatttttc ctttgtcttc tctaatactc aggtcaaaac gctgatttca
    10021 caacctcttt agcctactat tgaaaattac aacttacttg ctgcttcttc atcgatgatc
    10081 acaacaacat tgtcgtgatt ttgcaataca cttgctggca aatgattcgt tacaggtcct
    10141 tcaatcatcc ctttgattgc atcagctttg ctttcgccaa atgccatcaa gacgatttct
    10201 ttacttttca taattgaacc gattcccatt gaaacagctt ttgttggtac atcttctgct
    10261 ttatcgaaga atcgtttgtt tgcattgatt gttgattctg ttaagtcaac tactgaagtc
    10321 gttccgtcaa atgaagcacc aggttcattg aaaccgatat gtccattttg cccgattcct
    10381 aagatttgga tatcgattgg atgtgcatcg atgatttgat catacttttt gcatgctgct
    10441 tccagatctt ctgcttttcc atctggtaca aacgtttctt tgaatggttt tttatcaaat
    10501 aattggacat ccatgaaatg acggtagctt tgttcatctg aaccgcctag tccaacgtac
    10561 tcatctaagt ttacagaagt catttcactg aaatccaaat cgcttgaagt catttcttta
    10621 tataaagtaa ctggtgtgct tcctgttgcc aatcctaata cttttgcacc tttgttcata
    10681 ccttctttga tgatttcaaa tgcttttttt ccgccttctt cagcgttttt tacacgaatg
    10741 atttccatag tgattgctcc tttatatttt ggtatagccc aattatagca ttcttttttt
    10801 ctttttacaa taattagtat agaccaaatt gcattttttt cattttcttg acaataaata
    10861 ggaatccgtg ttaggtttca taagatggtt caatcaaaca atccatgttg catgaaaacc
    10921 aagcaagtga ttcactgtgg acgctttatg tctgcagttt ttttatgaaa aaggagttat
    10981 tatgagtata ctgacattaa acaacattac ccagcaattt ggcgacaaaa tcctctatga
    11041 aggtgtcgat ctgcaattaa atgctggcga acatcttggg ctgattggac aaaacggagc
    11101 cggtaaaagt acattgatta aaatcatcac tggtgagatc ttaccagatg acggacagat
    11161 ccattggcaa aaaaatattc acaaaggtta cctcgatcaa tatgtggaag tcgatgaaac
    11221 attaacaatc gaagaatttc taaaaactgc gtacgcaaaa gaatacgaaa aggaagaaaa
    11281 aattgctttt ctttaccaaa catatagcga gaccttcgat gattccttgc tggaaaaagc
    11341 tgggaaactc caaacggaat tagaccaagg agtgttttat caaatcgaca ccttagttgc
    11401 tgagatgagc agcgggttgg ggatcgatgt cttaggttta gattcactgt tgaaaaatct
    11461 aagtggtgga caacgttcaa aggtgatttt agcaaaacta cttttagaaa acccagatgt
    11521 tctgatctta gatgaaccaa ccaatcactt agatgatcag catatccaat ggctgacgca
    11581 gtttcttcag gattttgacg gtgcgtatat cgtgatttcc catgaccaag aatttcttga
    11641 tcagatcacc acccatatcg cagacatcga gtttggaaag attactaagt atactggcca
    11701 tctaaaacaa gcattgaaac aaaaggaaca aaatcgcgaa agctatttgc gccaatatca
    11761 tgctcaacaa aaacaaatcg aaaagaccga agcttatatt cgaaaataca aagccggttc
    11821 ccgatcaacg atggcaaaaa gccgacaaaa acaattagat aaaatcgaac ggctgacgcc
    11881 tccagcttca gcaagtaaag ctgtctttga ttttccattc tcgcctattg tcacgacatt
    11941 agcagtggaa acaactgatt tagtcatcgg atatgatcga ccgctacttg cacccatcaa
    12001 tttgaccatc cgctttggtg aaaatattgc cattcgaggc ttcaacggga ttggaaaatc
    12061 tactttatta aaaacactga tcggtgagat tgaaaaaata agtggcgatt tccacttccc
    12121 agataacaca aaaatcaatt acttttcaca agatctgtac tgggaaaatc cattagaaac
    12181 accactacaa tatttaagca gtcaatttcc aaaagcgact atcaaagaat tacgtcgcca
    12241 actagctaaa gcagggttag ttaatcaatt agcaagtgaa cctctcacta cattaagtgg
    12301 tggggaacaa actaaggtga aacttgcaca gttgacaatg aaccaaggta atttactgat
    12361 tctcgatgaa ccgacgaacc atatcgatca agaaacgaaa gaaagcttac aagacgggat
    12421 acagaaattt ccaggaacag taatcatcgt ctctcatgaa caagaatttt atcaagatct
    12481 tgtgcaacga gtcattgaga ttgaaccaaa ataaacgttt gattgaatag ttgaaaaaaa
    12541 ggagctgtga caaaagtttt gtcacaactc cttcttctga ataaatggtg gccaattagc
    12601 tgaccttcgg tattagctca aaaaacggca aaatatgagg agctattttt atttggctat
    12661 tcttattact tgatgcagaa cagcttttca caacctcttt tcgtctattt tttattttcg
    12721 agatttctct ttcctttaaa aacaactgtt tgaaagaaga acgcacatcc ttgaaaaaca
    12781 agcccgctaa ccgttacacc taaccagcca tactgctgcc acattgtcgt ccctagcaat
    12841 gaaccagccg cacctccaat aaagtacata aacataaata cagtattatt tcggttgctt
    12901 gctgcttcac ctagcccttg tactcgtgtt tgattggcga cttgaccaaa ttgattcccg
    12961 acgtctaata aaataatagc taaaagcaaa atggccaaat ggcttccgcc aaccaataaa
    13021 gcaataaagc ttattgtttg cataacaagt cccatataca cgatttttct ttcagaataa
    13081 gaatctgcca gtcgtcccac gataggtgca gcaactgctc cagacaaacc aaaaatcgct
    13141 aatatccctg cttcgaatgt tccccaattg taggcgggac tgctgatata aaaaatcaac
    13201 gtagaccaaa agatcgaaaa tgttccaaac atgaaaaatc cactgactgc cgcttctctt
    13261 aatagacgtt gagaagcaac taaatgcggc aaacttttaa gtgaatcaag gtatgaaaca
    13321 gctgtactac tttgctggac agaacgcggc attttccagt aaagcagaag tgtaagaatt
    13381 ccgactgcac ataaagcaaa taaatagatc atccgccaag aaaaaagact ggcaacaata
    13441 ccagataccg tccgagaaag caaaatcccc acgagtaaac cgctaagcaa tgtacccatg
    13501 accttccctc ttgcttttgg tcccgctagt accgcaccgt aaggaatgat gatttgcggc
    13561 acgatcgaaa gtaatcctat taaaaaagaa gcgcatgcaa ataacgtaaa cgaaggcgca
    13621 aaaaaagcgg ccaacagaga gacggcagat aacgctgcca ttcgaatgat caattttgga
    13681 cgatccacca cgtcccctaa aggaactaaa aacagtaacc ctaacgcata gcctaattgg
    13741 gtcaacatcg tcaatatccc gactgcactt gtgctaacag ataaactagc tgctactttt
    13801 gttccaatcg gctggatgta atacatattg gcaacaacaa cgccacaagt gattgataat
    13861 agtaaaataa gcgaagttgt taaaggtttt ttttcattac tcttcattga tttcccccat
    13921 ttcctatctt agtttacaac ttatggaaaa gattggaaat agaaaaatac aactattcgc
    13981 cacacttatt atttgtgctt ttgttttcct tgttttttct tgcgatcttt ataaatgaag
    14041 acaactaacc ctactagcac agtaacactt gcaaccaacg tgactgcagt tttgttatat
    14101 ccgtgaaaca gagcttctcc accacacgca tacaacacag acgaagggat gactccaatc
    14161 acgatcgaca atatgatttg ctgtagaggc aatttcatcg catctgcagc aaaattgacc
    14221 atagaagatg gaataaccgg tatcatatag cctaacatta cacctatttt tggatgtttc
    14281 atttgtcgga ttgcttgcac ccaatgattc gtttcggttt tcttatctaa aaattttaag
    14341 cgatgcataa ggaaaattgc agtgatgtta ccaagagcat tacccaaaat gttgatgatc
    14401 gcacctgtca gtggtccaaa gctcaaacca gcaatgactc caactactga agtaggaata
    14461 cctggaacag cacacatcac cgctgtcaat acgatcaaga gtatcgatgc aaaagtccca
    14521 tgcgaacgaa tatgtgcgag tagctctgcc ttactcgctc tcggatttaa aaataattgg
    14581 atgtctgtct gatattctaa atacaaccga tagcccacta ccagtatcag aatgattccc
    14641 aacgagatca atatcaatcg gattattttc tttttcatat ttcctcctga aaaaaatcta
    14701 tattcattaa ataatagttt aacataaaaa caaaaaagca ccgaaatcca acataggatc
    14761 tcagtgcttt taaatagtaa aaattattta acagttacag atgcgccaac ttcttcaagt
    14821 ttagctttca attcttcagc ttcttcttta gatacgcctt ctttaactgg tgcaggagcg
    14881 ccatcaacta cagctttagc ttctttcaag cctaaaccag ttgcttcacg aactgctttg
    14941 ataactttaa ctttttggtc tccagctgca gttaattcta cagtgaattc tgtttgttct
    15001 tcagcagcag cagcgccacc agcagcagct acagctacag gagcagcagc agatacgcca
    15061 aattcttctt caatagcttt aactaggtcg ttaagttcta ggatagttgc ttcttttaac
    15121 tcagcaacga tgttttcaat gttcaatgcc attataattt cctccgttta attgttcttt
    15181 tttatttaat gctttatgcg gcaattaagc tgcatctcca tcttctttgt ctgccactgc
    15241 tttgacagcg taagccacgt tgcggactgg cgcttgtagt acagatagca acatagatag
    15301 aagtccttcg cgatttggca attttgccaa tgctgtgatt tcttccaatg aagagacttt
    15361 accttcgatg ataccgcctt tgatttctaa tgcttttgct tctttagcaa attcgtccat
    15421 gattttcgct ggtgcgacta catcttcgtt actgaatgca acagctgtag gtccagtgaa
    15481 tacttcgtcc atgccttcaa ggccagcttt tttagctgca cgtgaaagaa tagaattttt
    15541 gataactttc atttctacgt tagcatcacg aagttgttta cgtaaacgtg ttacttcatc
    15601 aactgttaat ccgcggtagt caacgactac tactgatgca gctgctgtaa atttttcgct
    15661 tacttcatca acgattgctg ctttttttgc gataattgct tcactcattt ttgagtttca
    15721 cctcctgaat tttgatggaa gagttttttg cactgaaaag gcaagtcact tttatagtta
    15781 ctttgctcaa ccagtattat tcatcaccta ttcacttcgt tcatagtgat tctactcgct
    15841 ttgctcgaaa ggaaactcct ttcgctttgc tcaaccagta ttgttcatca tctcttcact
    15901 tcgttcatag tgattcacaa taaaaaactc tatgccaccg cagacataga gggactattg
    15961 aatatattca ataaacgtcc tcggtaggaa attaaggctt gatgccacct actgtcttcg
    16021 gtacaagtta attattaacc tcaactagct tagcatgcta aactagttga gtcaagaatt
    16081 atttttcagt tggatcaatc aacattctaa tatttaatta gaaagaagct tggtcaacat
    16141 ggataccagg tccaaatgtt gttgtgatcg ttacattttt catgtattga cctttagctg
    16201 ctgatggttt agcttttagc aatacatcat ggattgctgc aaagttttca actaattttt
    16261 catcgtcaaa tgaaactttt ccgattggta catggatatt tccagctttg tccacacggt
    16321 aagttacttt accagctttc acttcgttta ctgcttttgt tacgtccatt gtaactgtac
    16381 cagttttagg gtttggcatt aagccttttg gtcctaatac tcgtcctaga cgacctacag
    16441 tagccatcat atcaggtgtt gcaacaacta catcaaagtc aaaccatccg ttttggattt
    16501 tttgaaccat gtcgtcgtca cctacgaaat ctgcaccagc tgcttcagct tcttttgctt
    16561 tgtctccttt agcaaaaact aaaacagttt gtgttttacc agttccgttt ggtaatacaa
    16621 cagcaccacg gatttgttgg tctgcttttt tagggtctac gttcaaacgg tatgcaactt
    16681 caactgttgc atcaaatttt gcaatgtttg tttcttttgc taaagctact gcttcggcaa
    16741 caggataaac tttacttgaa tcaacttttt ttaatgcatc ttgcattttt ttgctctttt
    16801 tagccatttt ggttcctcct tgattgtggt tataacggtt gtacctccca cgtgttctat
    16861 atctttcaat atagagccaa ctgagaagct gctgtcttag ggtgtataga ttattctaca
    16921 gtgatcccca tgcttcgtgc agttccttca accatgcgca ttgctgcttc aacgttagct
    16981 gcgtttaggt cttccatttt taattcagcg atttctctta cttgatcgct agaaactttc
    17041 gcaactttat ttttgtttgg ttcgcctgaa cctttttcga ttttagctgc ttttttcaat
    17101 agaacagcag ctggcggtgt ttttgtaacg aatgtgaatg aacggtcttc gtatacagaa
    17161 ataacaaccg gaatgatcaa acctgcttga tcagctgtac gagcgttgaa ttcttttgtg
    17221 aatcccatga tattgatacc cgcttgacct agtgcaggac ctactggtgg agctggagtt
    17281 gctttacctg caggaatttg caatttaacg attttttcta ctttctttgc cacgagacat
    17341 acctccttaa gtccgtgatg tggttaattg gagatgcata tttctcctcc cacgttttat
    17401 ctgatctgaa aaaatccaga caaataatgt gcaaaacaat gcacactggt acagtatagc
    17461 aaatttattt gttttttcaa gatttttttc tgctattttt acattttgct tgaataaacg
    17521 ctaaatttat tctaaaaaaa caacaatttt tcgttctcat ttcttctttt atgttacact
    17581 tgcgctagca tggttcaaca gatgaaagca aacaataaca cggtttttta gacccttgta
    17641 caaagattcc tacataacaa aatgaggtgg agaaaatgaa agatttaagg tatagaagtg
    17701 tctttgatat tattggacct gtgatgattg gtccaagtag ctctcacact gccggagctg
    17761 ctagaatcgg gaaaatcata cgtagtatat ttggcgaaca gcctgattct gtagatattt
    17821 atctatatga atcatttgca aaaacctatc gggggcacgg aacagatatt gcattagttg
    17881 gcggcctgtt agggatggaa cccgatgatg aacgtttagc tgattctttg aaattggcat
    17941 atgaagccgg tatggaagtc ttgtttgttc ctaagagtga aaaagccgaa catccaaact
    18001 ctgtaaagat gcttctaaaa aaaggaacac gaaagctttc agtcactggc atttcgatcg
    18061 gcggtggcaa tatccagatt tctgaattga atggattcaa aatttcttta agcatgggta
    18121 ctcctacctt gatcgttgtc caccaagatg ttccaggtat gattgctaaa gtgactagta
    18181 tactttctga gaccaatacg aatatcggca ctatgacagt gacacgggaa tcaaaaggtg
    18241 aaaatgccat tatgataatt gaagtagatg atcctcaagt agaagaaacg gtaaaaagac
    18301 tcaaacagct gccaaatatc gacagcgtaa attatttcga ttaaggagct aaaaaaatgt
    18361 ttttatcgat tgaagaatta gtagatcaag caaaagctta tccaagtgta gcagaattga
    18421 tgattgcagt tgaaatggaa atgagtggcc gttcccgtag tcacatcatc gagatcatgg
    18481 aaaaaaacct ctcagtaatg gaacaatcaa tcaacgaggg aaaagaaggt gttacctccg
    18541 tcacaggaat taccggtggt gatgcaaaaa aattagatga ttatttaaat gaggcagact
    18601 ttttaagtgg agaaacaatc ttaaaagctg tacgcaatgc tgtagctgtt aatgaagtca
    18661 atgcaaaaat ggggttgatt tgtgctacac cgactgccgg aagcgcgggt gttgttcctg
    18721 gtgtgctgat ggctgtccga gatcggcttg atttgacaca tgaacagcaa ttggaattct
    18781 tatttaccgc tggcgctttc gggttagtca tcgcaaacaa cgcttctatc agtggtgcag
    18841 aagggggatg tcaagcagaa gttggttctg caagtgcaat ggcagcagca gctttggttt
    18901 gtgcaaaaaa tgggactcct catcaagcag cacaagcagt agccattaca ttgaaaaata
    18961 tgatgggact gatttgcgat cccgttgcag gcttagttga agtcccgtgt gtcaaacgta
    19021 atgctttagg ctcttctcaa gcttttatct ctgctgatat ggcattagcg ggtatcgaaa
    19081 gtgtcattcc gccggatgaa gtcgttgctg caatgtacca agtcggacgt caaatgccgt
    19141 cgattttcaa agaaacagca gaaggtggct tagctgttac gccaactgct aaacgacttg
    19201 cggaagagat tttaaataag agggcgtgat aaagctcctt aatttcgagt attaagagat
    19261 tagttatctt cataacaaaa aacgaagaga ggactaattg cccttctctt cgttttttat
    19321 taatccatca atttcgtatt ttttacttca tcgatcgttt ttgttcctgc aagttgcatc
    19381 gtgatcgaca attctttgtt caggtgttca aaaacagatg ttactccttc tgccccacct
    19441 agattcaacc cataaatcac tggtcggcca atcgcaacta agtcagcacc acttgccaga
    19501 gctttgaaga catgctctcc tcgacggaca ccagagtcga aaatcactgg tacacgtttg
    19561 ttgactactt cggcaatttt tggcaatact tcaaaggacg caggacctcc atctagttga
    19621 cgtccaccat gattggatac ccagatacca tctgcccctg cagagatagc gattactgca
    19681 tcttctggcg attgtattcc ttttacgata actggaagat gggtgatttc tttgattgtt
    19741 ttgatgtcat ctggtgtcaa gccttgtttt gcagctgcat aaatttctgc gattcctttt
    19801 ccttcgccat taccacttgc actttgttcg ctatacgcag ctaagttagg cattggtaat
    19861 ggaaattgga attgattgat gacgtcttct tcacggtaac caccgagcgt tgaatctgcc
    19921 gttaaaatga tagctttagc ccctgcttct actgctttgt tcaaaatgaa ctcattgaat
    19981 ctgtcgtctt tactcatata taattggaaa aactgcggtg cgcctggcgc tgcatcggct
    20041 gcgtctttga ttgtcgtatt tgcataagtg ctgatggaaa agattgatcc agcagcagct
    20101 actcctttag ctgtatcagc ttcccctttt tcatgtgcca atccttgggc cgctgatggc
    20161 gcttggataa tcggtgtatc taattcaatt ccgaaaacag atgtatgcaa atcggctgag
    20221 tcgattccgc gtaatatccg tggcatgatt ttctttgtat taaaagaagt cgtattttct
    20281 ttcatcgtcc actcatcttc agaaccaccg cgaatatagc caaaagctcc tgcttccatg
    20341 cgctctttta cacgaccttc tagttaggca atgtttacaa tatcgattgg atgttcttca
    20401 ttacttgtat gataagtcat agtagatcct tctttcatct tcttgattac atataaatca
    20461 tactaaactt acaaaaagta agcaaacatt tactctcaga tacttttatc gctattcttt
    20521 ccaaataaat cgacttacaa gctgatctac ttcttggttt tcatgaagca tactatgaga
    20581 ggcattttca cctgtcacta ctttttcctc tatttcattt ccgcgttgct tcaacaacgc
    20641 atagaccgct aatgcactgt ttaacggtac tgttccatca ctcaagtctg tttcatctag
    20701 ttgaccagca atcaagaaaa atcgagtggt tataggaata ttaccgatca tttgctggta
    20761 atcttgatag cggttagaaa cgacagcagg cccctttgct aactcatctg tcagagactg
    20821 cgcactatca tcgacaaaat cattaaaagg cgcgccaatt gctgttaatg tctgaacttg
    20881 tggcaatgac gtatcctgtc catacgttcc taaatagcgt agtgcactaa ctcctcccat
    20941 ggaatgcccc actatgttga ctttatctat gccgtagttt tctttcagat acaaaagaca
    21001 agttttgatc cattctgctt gattccactc gttattttta ttatcttcaa acaatacttg
    21061 gaccatcgga ttgtctgctt ttccagacaa atctcctgtt gcttgtattt gtccatctgt
    21121 tcctactgtt aaaaccaatt cttttactgc ttgctgattt ttttccaaac gccgcaacat
    21181 ccctttaaag gaattttctg tcccgctgta gccgtggaaa aaaagtgttg gaatagtggt
    21241 tttttctatc actttactgg attctgccgt tttttcttta tcagaaactt cagtagtatt
    21301 tatcttcgtc gtctggcaac cacttaacgc aaacaaactg ataaggacta aagctgctgc
    21361 agttgcacgt ttgcataaat ttttgattgg ttctgtcata cacctcttcc tttccatata
    21421 gaaaaaagaa agacttgaaa ggccaatcat atgatcgaca tttcaagtct ctttattaat
    21481 cgattttatc tacttgatcg aaatcaagtt ctgtactggt ttcacgtcca aacatatcga
    21541 tgttgacttt caatttttct ttttctccgt caacttctgt tacttgtcct tctaatccag
    21601 caaatgcgcc ttcgatgatt ttcacgactt cgccaacttc tacttctaga tcattttgtc
    21661 gcaggctcat accgatagaa cgcaagatat gattgacttc ttctggaagt aatggtgctg
    21721 gtttgcttcc ggctccgtgt gaaccaacga atcctgtcac accaggtgtg ttgcggacaa
    21781 cataccatga agcgtctgtc atgatcattt caacaagtac gtatcctggg aatgttttat
    21841 gaacgatttc tttttctttc ccatttttga cttcagtttc tttttcttca ggaacaacga
    21901 cacggaaaat aaagtcggcc attttcatac tttgcgcacg agattcgata tttgctttta
    21961 ctttgttttc atatcctgaa tatgtatgca agacatacca gtttttttca aaagattcca
    22021 ttgaaggctc ctcatttcat tgtaaaataa aaaaacctca gtacacccga agtttttctt
    22081 tcgcttcatt atagcactaa aagtacgttg tggctagcga gttattttaa aatccagcca
    22141 aataagcttt ggatggctgt atccattacg taaaacatca atccgaaaat aatagaagtt
    22201 tcgatgacta ctaacgtatc tttacggagt tgttttctgc ttggccaagt tacttgtttg
    22261 atttcatctt ttacgctttt tagaaatttc atgagacaac ctcccttgct actgtttatt
    22321 tcgtttcttt atgtaatgta tattgattac aatatttaca aaacttattg atttccaaac
    22381 gttcgccgct ttttccttca ctgactgatt ttgtgtagtt gcgagaccca caaacggaac
    22441 aagcgagtgc tgcttttttc tttgccattt acttctgccc ccttgctgag aatcagactt
    22501 atacttcata acagtatcac gtaacttttt acttgtcaat ttgtgaaata aactatctat
    22561 tgttttggat aaaattatga tatgattaat ttgaaattaa gctaaaatga gacaattgga
    22621 ggtatagcga tgctattaaa aacacttgta tataagaaaa aagatctgac aaccgttaca
    22681 gagactagta cattagaaga agcattaaaa attttagaaa cttccggata ccgttgtgtg
    22741 ccgatcttag attcttcagg taatattttc cgtggaaaca tttacaaaat gcatatctat
    22801 cgtcataaag caaatggcgg tgatatgacg cttcctgtca ctcatctttt gaaaaacgct
    22861 acaaagttca tttacctgga tacttctttc ttcaaagtat tctttacgat caaagagctt
    22921 ccttatattg ctgtccttga cagtgaaaat catttttatg gtattttgac acatagtacg
    22981 ttgcttaata tgctctctca atcttggagc gtagaacaag gaagctatgt actgacaatt
    23041 gcttccacag gaaagcaagg agatttggct gctattgcaa aaatcatttc aaaacacagc
    23101 agcattgcca gctgtattac tcttgatatc gaaaaagacg aatacattcg acgtacattg
    23161 atcacgctac cggctaacac tgaagcgaaa gtatgtgaaa cgatcgtcaa ccacttagag
    23221 aaaaagaatt ataaagtggt agaaattgaa aatttgcaaa accaagaagc ataatcaaaa
    23281 agcgaaaaaa gaaaacagct aatctgcttt cttttttcgc tttttataat tagacacgct
    23341 tttcaaaatc aagcgcgcga tctaaataat acatcaatgg tgctgaaaca gccatgacga
    23401 taaactcact taatgcaagc gtgccatagg ttgtccaaaa tgcttggcca cctgttggaa
    23461 ctaacatata agcaatcagt cccatgctga tggtaaagaa gatcgtgttc aatactaaac
    23521 gcatctttac atttttcact ttgttttgca ataaagctgt caggcctaaa gaaagcaatg
    23581 tttggccgcc accaaaaacc acatccatga taccaaaacc aaagaaggca ttatagacga
    23641 tgaccccgcc taaaactccc caaagcaatt tacggttaaa gacaactaaa tgattcaaac
    23701 tttctgaaat acgaaactgg atcggccctg aggatacagg cgcaactaaa atagttaacg
    23761 ctagataaag agccatgatg atgccattta caacgattac ggataaccgt tgtctgcttt
    23821 tcgttttttg cattttctct cctcttttct cctagttttt ttaacgcggg atggttgatg
    23881 aaccgcggtt atgtgaaaca aacaataatt ataacagact tttcatttat ttcaatccgt
    23941 cttatctgct tttaaaaaag caaaagctta aacaatgtaa acgtttgcta aaaatcgtgg
    24001 gatatggtat aaagtaagtg taaggggtca taagaattcc cagtaaagca gacgaagtag
    24061 gatctgcaag aacagaattg ttgtgaaact taaataaata aaaaaggatg tgggaatttg
    24121 agcaacctct tactagagaa cggtctagca agcagctgaa gatctaagtg gacatgccga
    24181 accttggaac ttctccttac gtaagtaatg gatcatgcgc ttgctaggct t
//
LOCUS       DAYYAR010000029        23945 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000029 DAYYAR010000000
VERSION     DAYYAR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.029"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..3344
                     /locus_tag="ACHAT0_001028"
     CDS             66..3344
                     /locus_tag="ACHAT0_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8450986.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3885..5792)
                     /locus_tag="ACHAT0_001029"
     CDS             complement(3885..5792)
                     /locus_tag="ACHAT0_001029"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8450987.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5922..6797
                     /locus_tag="ACHAT0_001030"
     CDS             5922..6797
                     /locus_tag="ACHAT0_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8450988.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6877..7851
                     /locus_tag="ACHAT0_001031"
     CDS             6877..7851
                     /locus_tag="ACHAT0_001031"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8450989.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8031..9176
                     /locus_tag="ACHAT0_001032"
     CDS             8031..9176
                     /locus_tag="ACHAT0_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8450990.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9313..9657
                     /locus_tag="ACHAT0_001033"
     CDS             9313..9657
                     /locus_tag="ACHAT0_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8450991.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9928..11214
                     /gene="efmA"
                     /locus_tag="ACHAT0_001034"
     CDS             9928..11214
                     /gene="efmA"
                     /locus_tag="ACHAT0_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8450992.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11352..11951)
                     /locus_tag="ACHAT0_001035"
     CDS             complement(11352..11951)
                     /locus_tag="ACHAT0_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8450993.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12355..13476
                     /gene="mnmA"
                     /locus_tag="ACHAT0_001036"
     CDS             12355..13476
                     /gene="mnmA"
                     /locus_tag="ACHAT0_001036"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8450994.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13722..15143
                     /gene="arcD"
                     /locus_tag="ACHAT0_001037"
     CDS             13722..15143
                     /gene="arcD"
                     /locus_tag="ACHAT0_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8450995.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15157..16275
                     /locus_tag="ACHAT0_001038"
     CDS             15157..16275
                     /locus_tag="ACHAT0_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8450996.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16335..17627)
                     /locus_tag="ACHAT0_001039"
     CDS             complement(16335..17627)
                     /locus_tag="ACHAT0_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8450997.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17777..18337
                     /locus_tag="ACHAT0_001040"
     CDS             17777..18337
                     /locus_tag="ACHAT0_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8450998.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18466..18843
                     /locus_tag="ACHAT0_001041"
     CDS             18466..18843
                     /locus_tag="ACHAT0_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8450999.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18776..20848
                     /locus_tag="ACHAT0_001042"
     CDS             18776..20848
                     /locus_tag="ACHAT0_001042"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8451000.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21059..22006)
                     /locus_tag="ACHAT0_001043"
     CDS             complement(21059..22006)
                     /locus_tag="ACHAT0_001043"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8451001.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22252..22716
                     /locus_tag="ACHAT0_001044"
     CDS             22252..22716
                     /locus_tag="ACHAT0_001044"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8451002.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22778..23821)
                     /locus_tag="ACHAT0_001045"
     CDS             complement(22778..23821)
                     /locus_tag="ACHAT0_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8451003.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         7780 a         4394 c         5051 g         6720 t
ORIGIN      
        1 cagtaaaaat aaattaggta atactttaag taaacgcttt ctaatttgtt acgggaggaa
       61 tacacttgtt aaaaaaacgt ataaaataca gtgcgctaat gatgaatctc cttatgctca
      121 gtaccccggt tttaatttct gtcgttgaag ttgctgctaa cgaacaacag tctgtgaacg
      181 aagaaaatgc acacgtgaac attgaaggga tgtcaacaat ggaggaaata ccaagaacta
      241 gtgaagatat ggatacggaa gaaggaaatg atcctatgat gaaagaatca gaaaatgaaa
      301 agacaaaaaa gcaatctgaa gaagcaaatg aagagtggaa aggggttgtt ttcggagaaa
      361 gcacaagtgc tagtaggtat gccattgaac cattagaaga cggcgtgcgg ctctcttcca
      421 cgaataacgg cggaaaattc cagtcaagcg gatcagatgg aatgacctat tattatcaag
      481 caatccccaa agacatcaac tttaggatgc gggcgacgat cgaaatagaa tcttggacct
      541 atacgaatgc ccaagaagga tttgctttaa tggtaagaga cgcagtacca aaaaatcatt
      601 tgtttgggca ggcattttat tcaaattcat ttgccatact aggcagtcgg atcgaatatg
      661 tctgggactc ggatagacaa gaagtggtca acacgagcgg cagcaaatat acgatgcgac
      721 ttggattggg gacacgggca atcacaggaa tatcatctga agatccgcaa gctgcgccag
      781 ctcctgggag tgtctcagtg gaaacaacac ctttagagac ttcagcaggg ttcttggaaa
      841 aagaaacagg ttcttataat atattcggaa acggtcatgg gaatctgttt ccggccacga
      901 actcgattac taaactggac gtagagatga aaaaaacgaa tactggttta gaggcatatt
      961 attacgatcc tgaaacagga gaagaaatgg cttctgacat catgtatgac tgggaaaaat
     1021 tatacgcttc agatgaatcg gttatttatg caggatttgc tgcagcaagg aatatgacga
     1081 tcaaggtaga aggaatcgaa gttgcttact cagatcctgc gacagatcct cctgggcaag
     1141 aaagacctac tcgcttgatt gatccgattt acactgtgac ctctagtaat cattcaggaa
     1201 accaagatca tactatgtcc tttagagcaa atgctgacgg attattgaca attaaaaata
     1261 aagcaacagg agaggtgctt aagaacgcaa aaaacttggc tatcactgcg aatcgggaat
     1321 ttgattatca gacaaaactg atggaaggaa cgaacgaatt tactttcagt tttacgccag
     1381 ataaaaacta tccgccagaa gaatacgtag agatgactag ttatgaaaca aaagagattc
     1441 ttcatacagt ggactaccgt aaacctaaat attcgacagt ttatgtaaca ccagaagggt
     1501 cagatgcagg aaacggaagc agacaaaatc cattatctct gacacaagca ttgagatacg
     1561 catatgctgg gcagacaatc gtcatggcgc ctggaactta ttccttcgaa agaggattga
     1621 cgattccaaa aggcgttaac gggacaagag aaaacccgat tcagttgatt gcagagaaaa
     1681 atccagaaaa caagcgtcct gtacttgatt ttaaaggaac tggaaatggg atgacactgt
     1741 tcagtcacta ctggggttta cgagggtttg acattacaaa tagtgcagct atgcaaaaag
     1801 gattacagat ttctggaaat cataatttga tcgaagagat tcatgcttat cggaatggaa
     1861 atacaggaat ccaaatcagc ggtagctcaa atgatccttt cgaagcatgg cccgcgcata
     1921 acttagtcaa gaactgtaca tcttttgaaa atgccgatcc aggatttgaa gatgccgatg
     1981 gatttgcggc caaactgaca ataggagaag gaaatgtatt tgacggttgt atcgcgtatc
     2041 ataatgcaga tgacggttgg gacctttttg ctaaaaatga aacaggttcg atcggaaaag
     2101 taatcatcaa aaactcagtt gcttatcgaa acggctgggt accaggaatt gagggagaag
     2161 gtaacggtaa tggattcaaa atgggcggtt cttctttaac gggccctcat gaattgatca
     2221 atagtttgtc tttcgaaaat cttgcgaaag gaatcgattc caatagcgga acagatatta
     2281 tcgtcaatag cagtacaagt tttaataacg gttcatataa tgtggctctt tatacctctt
     2341 cagcaggaaa cacagactac catgcttctg gtatcttatc tttccgaaca gagaatcttg
     2401 agatgagaga gcagatccgc ccattgaatc aaaacgaaga tcaaatctac cactcgttga
     2461 actactattg gaatgaacga gagaaacaat cagaaaatac tcttggacat accatatcaa
     2521 aagattggat cgagtctcta aatacatgtt cgaaagaagg tcagcagcct gttacgcgct
     2581 atgaaaatgg aagtatccat gttcatggac tgatgcagat taaaccgggg aatcgtcaaa
     2641 cagaaaagga aagagtggat ggtcttccat tatctgtagg tgctattttt gacggagaaa
     2701 cctcaccgtc ttcggaatat ccagagtatg caatcgtaca agaaccaaat cagacaaaac
     2761 caaatgaaca aaataatccg attattatac aagaagcaaa tggtgtggcg gagaatgcag
     2821 aaggaaatat cattgaactg aagcctttta cagtttttgt gtcaactttc acaccaaatc
     2881 aaggaacgcc tgtctttgaa gatatgcgtt atttcccaga aaagatccaa atcgttgaaa
     2941 cacagtcaag tgctcaattt tctaatcata ccgctatttt ttcagcacct tcaaaaggag
     3001 aatatacgct agaagtcact tatcgttcgg aaatatttga tggggaaaaa tggtctgctc
     3061 atacagaatc tgttacccag caaaaagcga tcactgtgcg agaaactgcc gatgtgcccc
     3121 cgggaaatga caaaccagaa agagaaaatc caggaaatga atcaccaaat ggaaaaccaa
     3181 atggaaaacc ttctcatccg gataggacag caaaagaaag tgaaaaaaat ggcactcaag
     3241 gactcccaaa aacgggagaa tttaataacc cgttgctagc attagctgga ggaattttgt
     3301 taatcggtgt agttgtttat gtcatgaaac agcgaaaaaa ataagtggaa tttctccttt
     3361 tatgatgtaa aacaattgac tgaggaaagt aaaaatagaa ggaaatgaga ccaatagatg
     3421 tctcacttcc ttctatcttt ttatagatga ggttctcctt gctgcttttg attggctgtt
     3481 cttattactt gatgcaggac agctttttca caatctctcc actaaacggt gttcaatcag
     3541 ctgaccctcg gtattagctc aacaaacggc aaaatatgag gaactatttt tccgtttttt
     3601 tctctaatac tcaggtcagg acgctgattt cacaacctct cgataaacgg tgttcaaaag
     3661 ccagtttctc ggtagtaagt caaatttatg caaaaatgtg gaaagccatt ttcacaaaat
     3721 ttttcttact acttgaaact aagcagcttt ttcacaacct ctaatacatg gtattccaga
     3781 agtaacttct tcgaaaaata agccgaaatg tcacaaaaat ttgagaagcg attttcccaa
     3841 attccttctt atttcttgaa gctgaccact tctggcacaa cctcttacca gattcgcact
     3901 cttttttctg gcgctagata catagcgtct tgttccgtga taccaaatgt ttcatagaat
     3961 tcgtcaaggt tttttggttg cacgtttgct cggagtttag caggggcatg gacatcgatt
     4021 tgcagtaaaa gttgttggta ttcttttttt gcttttgtac gccaaatcgt tgcccaattg
     4081 acaaagaatt cctcgataga aagatctgat tccgtttttg ctgcttccaa tgcacagctt
     4141 agaccgcctg catcagcaat attttccgaa acggttaatt ttccgtttac ttttccgtca
     4201 gcaaaaggca gaccatcgaa ttcttcgatc attgcttgtg ctttgttttg aaattgtgta
     4261 agatcttctt ctgtccacca gttgttcaag ttgccatatt cgtcaaataa agcgccgtta
     4321 ttatcaaatg catgggaaat ctcatgagcg ataacagctc cgattccacc ataattagca
     4381 gagcttgatt gttctagact gtaaaatggt gcttgaagga tcgctgccgg aaagacgatc
     4441 acattccgaa atggatgata ataagcattg accgtgtcag cactcatttc ccattctgaa
     4501 cgatccactg gtttgttcca tttgctgaaa cgatctttta atgtgagttt gctgaaaaca
     4561 agagtatttg aaagcaaagt accaccctga tctgcaggta ctgttttgaa ctggtcgaat
     4621 aatgctggga tcttatcggg gtagcctact tggataccta agttatttaa ttttatgacg
     4681 gctttttctt ttgttgcatc cccaagccag tcattttctt ctagacgttt tttataaacg
     4741 gcgatcattt ttttcaccat ttgttctacg tcttttttag cagcttcgcc aaaatatttt
     4801 tttccgtagt aatcgccaac tacttgatcg aattgtccag aggcaaggta atacgctgct
     4861 tttttctgag gcattgcttc gtctgttcca gacaatacgc gagaatagat tccgctgact
     4921 tggcggaatt cttctgatag ataaccacta agagaacgta ctgttttaat gatcatccag
     4981 cttttgaata acggaaaatg agcttctgtc aggatgtcat tcaaatgatc gaagtacact
     5041 ggatcagtta cgatcacttt atctggagtt gtcccaatca aacccgtgac aagttttttc
     5101 aagtccagtt tatctgtgta tttaataaat gtatcaaaag attgcggatt atacattttg
     5161 ctgtaatctg cgttttcttc tgcacttttt acatgaggag caatcaattt atcaaattgc
     5221 actgcttgtt ccgtgattgc ttctgcctga cttttttctt tgccggcaag ctctaataac
     5281 tgaacagcca tatcaaagaa aacgcctatt agttgtgcac cgttgggatg gtctgatgca
     5341 taatacgtct tatctggaag gaataaagaa ggcggataag caaagaaggc gttcgtttgc
     5401 gcattcttca tatctgcatc tacatccaga ttaaatggca atggtaatgt atccaacagc
     5461 cattctggaa attgcttatt caattcgtcg aaagactgca atgattcaac tttttcgatt
     5521 ataggttgaa gcggttgagc atgcaactca tttcttcggt cataatcgtt tgccagttga
     5581 taatatgcaa ggaaatgttt cattttttct gaagacactt tttctggatg ggcaagcaga
     5641 tcgtctgttt ctcccatcaa aagttggtcg atcccgtcaa ctaaatcttg gaaaccgcct
     5701 gtagctggtt tatctgcagg gatttttgct gttttttgcc attcttcatt gactgcttca
     5761 tagaaatctt gtttcaatcg ttctttgttc attcatcgat cgctcctttt ttgtttcgtg
     5821 tgcccattat cttacaaaat gaaggaatta acaaatagaa cataaaaaat tgaaaattcg
     5881 aacaatcaaa ctgatttgtt tttttcaatc cttcacttat aatgaaaaaa aggaggcgat
     5941 cccaactgaa tcaagtagtg gataaacctt tatatttcaa agtagataaa aaaattaaaa
     6001 aattagccag cactcagcag ctgcagtcaa gaaaaagtaa attgcttttt ttagctctgg
     6061 tttttgaaga tcaaagttat gtgattatcg atcaatcagg acatccggta gaatattctc
     6121 cagcaaaata tacttatcaa gagggtcttt cttgtaaaaa atggaagctg atcaatgaag
     6181 agccaatcga gttatctaga tggatcaaca gaaaagaaga tattcccgtt ttgatcgaag
     6241 aaaaaaggtc aggaaaggaa ctcgcaaatt gctgggtggg attgccagaa gaacgttttt
     6301 tgaggtataa gaaatgggct accccctcag gctatctttg tggtacttac gcggcagcag
     6361 ttctccttgc ctattatcaa gactatcgta aggggtggat gcttccaaat gagattcgca
     6421 agaaaaatac ggctgattcg ttggttttga cgaaggcatt gcgtagccag atccaaccac
     6481 taggacttcc tacgattcct tttcaggtta gtacagggat ttctagtttt ttaaagaaga
     6541 atggcaatca tgagcgtgca cgagcaactc ttctaggttc atggcagcga gctacaaaac
     6601 gtatacgtga aggcaaacct gtgatgattg gtattttgaa agtacttggc agtacatatg
     6661 gcaatcattg ggtgacagcg tacgcttatt ttgaaactga aacaggagaa cgttattaca
     6721 aagtacacga caactggggc gattatcata aagtcattcc tgccagttgg agtaacggaa
     6781 cagtttcact tccttaagaa tttgctgaaa ttaacagaaa atcttggaaa aatatgttaa
     6841 actatcgaag aatagagaag ttttggggag gactacatga gtaataagta tcaggatgac
     6901 acgttgaaga tttttagttt gaatggcaac cgtccattag ctgaaaaaat cgcgaaagtg
     6961 ttcggaacag aattaggtaa aagcgtcgtg aagcaattta gtgatggcga gatttccatc
     7021 aatatcgaag aaagtattcg aggggatcat gtgtatatcg ttcaatcaac gaatgcacct
     7081 gtcaatgatt actatatgga actactgatc atgatcgatg caatgaagcg agcaagtgcg
     7141 aaaacgatca atgtcgtatt gccttactat gggtatgcta ggcaagaccg cacggcgaaa
     7201 cctcatgaac cgatcactgc gaaattgatc gctaacttga tcgaagaagc aggggcaact
     7261 cgagtactga ctttagatct gcacacggtt caagtacaag gattctttga tattcctgta
     7321 gataatttat ttacgatgcc gttgtttgct cattattacc gtcaattagg tctgacggga
     7381 gatgacatcg tagttgtttc gccaaaaaac agcggtgttc agcgtgcacg tagtctttca
     7441 gaatatttga acagcacatt agccatcgta gatcatgcag atgatgcttc aactgagggt
     7501 ggttatgtga tcggtgatgt tcaaggaaag acatgtatca tggtagatga tatcttgaac
     7561 acaggagcaa cgcttgcacg agcagcaaat gtattaaaag aaaacggcgc aaaagaagta
     7621 tatgcctgtg cgtcacatgg attattgtcc gatcatgcga aaagtatttt agacaatgca
     7681 ccaatcaaag atatttgtat cactgattct gttttcacag aagaaaatcg tcatccagaa
     7741 aacttaacaa tcgttacatg ttcagaatta atgggtgaag cggtcaaacg catccatgaa
     7801 aatacaccga tgagtccgtt attccgtctg gaagaaaaag aattcgaata aacaaaaaaa
     7861 agagggaaag aagttgtgac agaagtgagc agcttcaagg agctgcgaca agacttttgt
     7921 cacagctcct tttttctatg cccatgaaac gtcctctaga caaaaatgag ctcttctttg
     7981 gtttgttcgc tattttatgc tagaatgtgc ctagcaataa tttggaaggg atgaatgaag
     8041 tgaaaactat ttatctggat catgcggcaa ctacccccat gcatccacaa gtaattgaag
     8101 cgatgacggc aatcatgcaa gacacgtttg gaaatccttc aagcatacat ggatttggtc
     8161 gccacgcgca tgaaaaattg gaaacagcaa gacaaatcat tgcagacagt ctgcaagcaa
     8221 aacctcacga gatcatcttt aacagtgggg gaacagaagg ggataataca gctatcttag
     8281 aaacagcgtt ctcgcgtcaa aaagaaggca gacatctaat cactacagct atcgaacatc
     8341 ctgctgttct taataccatg aaatatttag agacaaaggg gtttgacgtt acatatctgc
     8401 ctgtagatga aaaagggaat ctgtcgatcg aagaggtcaa aaaagcgtta cgagaagata
     8461 cgatcttggt ctctgtgatg tacgggaaca atgaaatcgg aaatttgatg ccaatcaaag
     8521 aaatcggtga acttttagcg gatcatccag cttatttcca tacagatgcg gtacaagcgt
     8581 acggaaacca aacgatccat ccagaagaat taggaatcga tttattaagt atctctgctc
     8641 ataaaatcaa tgggccaaaa ggcgtcggtt tcttatataa acgagacgga gtaaatatcc
     8701 ctgcattgct tttaggcggg gagcaggaag aaaagcggcg tgcaggtaca gaaaatttag
     8761 cagggatctg gggtatggca aaagcagttg aactgctgtc acctgaagaa cgtgaagcac
     8821 gtaacaacaa atataaagga ttccagcaat tgattcttga tacattagaa gcaaacgaaa
     8881 tcccttatca agtaaatggt gatttaacga ataaactccc tcatgtgttg aatcttcgct
     8941 taattggcga agcaaatgat ttgctactga tgaagcttga tcttaatggg gtcgctattt
     9001 ctactggttc agcttgtaca gcaggaaata tcgagccttc acacgtatta gaagcaatgt
     9061 atggagaaga tacaccgata ctaaaagaat ccatccgtat cagctttggt ttgggaaata
     9121 ccgaagacga catccagtat ttccttgatc aattagttcg tgctgtcaaa aaataactag
     9181 tgaagtcatt cttatatgaa cagatgtctg agagaacgcc ttcatttttt agggaactta
     9241 actgaaaaac ctagtaaaaa tcaggataaa cttttataat aaagagaaac taattttatc
     9301 gaggtgaaaa tcatggcatt tgaaacaaca gcaacggtac tgggcgcacc aattagctat
     9361 cgtttgcatc ctgatgcaaa aagatataca ttgcgcgata atggatttac agaaacaaac
     9421 ggaggcaatt atcaattgat ccgaccatta gatgccacgc cgcaaagcaa agaaggattc
     9481 aaattaaaaa tcactgtatc aaaagatatt caaacattga aaatgtcgat cactacagca
     9541 aatggattga aagcagtcaa tattttcaaa gatccaaaac agaaaatgag ccaagataaa
     9601 ttttattttc tgatggatgg gatgattagc cgtgggttat tcgaaaaagt cgaatgatct
     9661 gtggatataa aaagacaact agttttgaaa cggcctattg ttagatgact aactttgtaa
     9721 ggacgacgca aattagttgt cttttttatt tttaaattta acacatgttg ttatttttaa
     9781 tattttgctg atttattgtt tctaagtaaa gaaatttaaa ttgaaacgat tgttatttag
     9841 ggaaaaaggt attttttgaa aaaagaatcc cgtataatga ttgttgtgca aacgctatac
     9901 aaaatgaaaa agaaaagagg gatgtttatg gaaaatgaac agtcggtcgt tttaacgaat
     9961 tggaagagaa attatttgtt ctttttatcg ggacagtttt tgtcaggaat cactagtatg
    10021 gtcgtccaat atgcgatcat ctggtatttg acaagagaaa ccggttcggc gacaattttg
    10081 agttttgcta ctttactagg aatgattccc atggttttac taagcccatt tgtaggaccg
    10141 ctagtggatc gctgggataa aaaagctctt ttgattgtca cggatattat tgtagcgatt
    10201 ttcgcgctta ttttagcagt agtcggaacg atttcagaat ccttcccgat ttggctagtt
    10261 tttgtgtctt tgtttatgcg ttcggtcgca caaacattcc aaatgccaac gatccagtcg
    10321 attatgccga caatcgttcc atcttctcac ataacaagga cgaatggaca gttaggaatg
    10381 gtccaatcag cgaatttcat cattgcgcca gctctaggtg cggctttgtt ttcagtcgtt
    10441 cctgtcaatt accttatttt attagatgta ttgggggcag tttttggtgt cggcttattg
    10501 atttttgtga agatcccaaa agtttctcct gaaatattgg aagttccgct tactattttt
    10561 aaagatgcaa aatttgggct gcagcaattg atggataata aagggttatg gtacataacg
    10621 atcaatggtg catttgtgat gctgttgttc atgccagcaa tcagtctgta tccattgatg
    10681 acgctagatt actttggtgg ttccgttgga caggcgggag cagtggaagt agtctacgca
    10741 gtcggaatgc tcttaggcgg tgcactgatc agttttatag gaacatggaa agaccgaatg
    10801 aaacctatta ttatagccta catcattatg ggcctgacga tcggtgcaag cggattggtt
    10861 ccaaacgata gtcaagggtt cttgtacttt cttatcttga atgcaggagc aggttgtgca
    10921 acgccatatt tcaatacact gctgatggca atgatccagc agagttatga atcgaatgtt
    10981 ttaggtcgtg tcttagggaa cttcaactct ttgatgaacc tggcaggtcc aattggatta
    11041 ttatttgcag gaccattagc tgatcgttta ggggtagaaa aaatgttctt gttttccgga
    11101 atcgggattt tattatgcgg aatcgtttta tttttaacgt cagctgcccg aaattatgat
    11161 aaagaattac agaaaaaact ggtaaaagaa caccatgagc aaaaagacga atagtatgga
    11221 atggagttgt gacagaagtc ttgttacaac tccatttctg atcctaatgt tcctgtggca
    11281 ataagcctac tcaaatgaaa aatatgagta attatcttta tttgtttaat acttgatata
    11341 gggcagtttt ttcacgacct atcgtacttc ctctttgctt catagatttc tttgatttgt
    11401 tgctcagata acggagtgcc aaatggtagt ttttccttta agagttctct ttccggtgcg
    11461 tctacgccta cattgacatc gttctcgata ggtacgctgt aatgatggat atgtccatga
    11521 agattgcgga tagtgtcgtt aggcccgagc aacatcggat aatgagtcaa gtaatattgt
    11581 tgatggtcga atttaacgat ggcacctaca tcataaaaat aaaatttggg acttccgtca
    11641 gtaagcctag gatcattttt ttctaaataa cggaaaaaag cgcgactgtc atggttccct
    11701 ttgatcaagt ggattgttcc ttgcagttga gtgaccagtt ccaaaatttc tgcatttgct
    11761 ttttcatatt gcggatgcat agcaaggtcg cctagatgat aaacatggtc ggtttctttt
    11821 attactgcat tccagctagt gatcaactgt tggttcattt catccacatt tgcaaagagc
    11881 cgcggagcaa agtcgttttt tcctaataag cgttcatgga aaaagtgcat atcactgata
    11941 aagtaattca tgtatctttt gcctcccttt tttcttagag taaacaaaat cacttatctg
    12001 agcaactcat aacatctaaa aagaaaaggt tgtaaatgag acgtgcagct ttaagcgata
    12061 agaagcaatt ttgaaaaata gtttttcaca atttataaaa ttattcctta ttgccgaaag
    12121 gctggccctt tttatgagta tgccaatttt ttatatcgaa tgctcaaaag atgagtgatt
    12181 ttttggttga ctatcatgtt tgtgtactga ataggtccag tagaacagta tttttacgac
    12241 ttgataatta agggatgaaa ttttcagaaa tatgacgtat aatgtaaaat actgaaaagt
    12301 tgcgaccttc aactcgtaga tgtttcagaa tctaattgaa atgatggtga tatcatgaca
    12361 gacaacagca aaacacgcgt tgtcgtaggg atgagcggcg gtgtcgactc atctgtaaca
    12421 gcacttttgt taaaggaaca aggatacgac gtgatcggaa tattcatgaa aaattgggat
    12481 gacacggacg aaaacggtgt atgcacggcc acggaagatt ataaggatgt agcgaaagta
    12541 gctgctcaaa tcgggattcc ttattactct gtgaattttg aaaaagaata ttgggatcgt
    12601 gtatttgaat atttcctagc cgaataccgc gcaggacgta cacctaaccc agacgtgatg
    12661 tgcaacaaag aaatcaaatt caaagcattc ttggattatg caatggatct tggcgccgat
    12721 tatgtagcta ctggacatta tgcgcaagtc actcgcgatg aaaacggcgt ggtccacatg
    12781 cttcgtggag tagacaataa taaagatcaa acttacttcc ttagccaatt atcacaagaa
    12841 cagctttcaa aaacgatgtt ccctctaggc ggcatggaaa aatcagaagt acgggcaatt
    12901 gctgaacgcg caggccttgc gactgctaag aaaaaagatt caacgggaat ctgctttatt
    12961 ggtgaaaaga acttcaaaca atttttgagc aactatctgc cagcaaaaaa aggaaatatg
    13021 gtcactcttg atggtgaagt caaaggacaa catgctggct tgatgtatta tacgattggt
    13081 caacgtcaag gactagggat cggtggcgga ggagattcgc aagaaccctg gtttgttgta
    13141 ggaaaagatc tagcgacaaa cacattatat gtggggcaag ggttccatca tcctgcattg
    13201 tatgccacaa gcttggatgc aagtgaaatc cactttacga cgaatgaacc aatgccaaaa
    13261 gaattcaagt gtacagcgaa attccgttat cgtcagcaag acgttcctgt gacagttcgc
    13321 ttattggatg ataatcgtgc agaagtcgta ttcgacgaac ctgtacgtgc aatcactcct
    13381 ggacaagcag ttgtatttta tgatggaatg gaatgtctgg gtggtggttt gatcgaccac
    13441 gcatatcaag aaacaaaagt tttacaatac gtttaagcga tattaaatga atcaaaacag
    13501 ctatggctaa caaaagaaac accttttctt ttggatagca tagctgtttt tttgcaataa
    13561 aattaatttt tattcatttt atacttgaaa tacttgtgta aaaagttcat aatatccaat
    13621 aatatgaata aagttgcttt ttgttaagca agagcagttg caaaatagaa gggaatcttt
    13681 tgcaagtgat ttgttcaatt ttataagtga gggtgatgga aatggaaaag aaatcgacgg
    13741 gtattagcaa atggggactg gttgctctgg ttgtcagttc gtcaatcgga agcggtgtgt
    13801 ttgggatcac aagtgatctg gctagttctg cagctccagg gccagcgatc ctttcatgga
    13861 taatcgttgg gattggtatt ttagctttgg tattatcgct gaatcattta ggagaaaagc
    13921 gtccagatct agatggtggt attttcggtt acgcggaagc ttcatttggt aaattaggtg
    13981 gcttcatcag tggttggggt tactggttgt ctgcgtggct gggaaatgtt gcttttgcaa
    14041 cgatgctgat gagcgccatg ggagaatttt tcccgatatt caaaggtgga caaaatgttc
    14101 cttctatttt atttgcaagt atttttattt ggggtctgac tttattagta aacaatggag
    14161 ttgaaggagc gagcgtcatc aatacaatcg tgacgatctg taaattagtg ccattattcc
    14221 tatttttaat atttgtattt atcgcattta aagtgaatat attcactgct gatttttggg
    14281 gtaacgtatc tgataatcta gtgaatggaa cagggcagca aggaacgatc tggttgcaga
    14341 ttaaaggttg tttgatggtt atgatgtggg tctttgtagg gatcgaaggc gcaagtgttt
    14401 tagccaaccg cgcgaagaaa cgttcagaag cccagcaagc atcgatcatt ggtctgcttt
    14461 gtttattgtt tatctatatc cttgcttcga tcttgccata cggcgtctta tctcaagaag
    14521 agttagcaac tatttcacaa cctgctatgg caaatatttt aaaaggaatc gtgggaaatt
    14581 ggggagcagc gctgatcaat atcggcttga ttatttcgat cattggttct tggttgtctt
    14641 ggacgatgct cccagcagaa acaacgatgt tgatggctcg agacggtctg ttgccgaaag
    14701 gttggggaaa aacgaataaa aaaaatgccc caacttattc gttagttgtt actgcaatct
    14761 tgactaatct attcttattg actttcttag ttactgatta tgcctatcaa tttgcctatt
    14821 ctttatgtac agcggcaatt ttgatttgtt atctacttgt cgggatctat caagtccagt
    14881 tttctttaaa agaaggaatc aaaaaacaag tagtgatcgg tgggattgct gtagtttttg
    14941 aattgatggg catcatttta gcaggatttt cttatgtcct tttatgtagt attgcctatc
    15001 ttcctggttt ttgcttctac tggatcgctt gtcgtgaaac caaacatcaa attacgggaa
    15061 aagaaaaagt aatgattggg ctgatttgtg tggccgcact gctttcaatc gggttgcttg
    15121 ctgtaggctg gatcaagata taagggggaa agaaacatga aaatcgcaaa tttcggtgta
    15181 gaagaatggc taaataaatg ggaaaagaaa gcggtatatg atattgcaca aagctcgatc
    15241 gaagcattga cattagacga actcgttggc ttagatggga ctagcgtaag taaatttttc
    15301 gaaaaacgca aaaacgaacc attggactat ggctggatcg aaggatcaga tgttttcaaa
    15361 caagaagtcg cttctcttta tcaaaccgtc gatccggaaa acatcttaca aacgaatgga
    15421 gcgactggtg ctaatctttt agctctatac gcgttagtgg aagcaggaga ccatgttgtt
    15481 tcgatgcttc ctacttatca gcaattatat gacatcccca aatctttagg tgccgctgtt
    15541 gactttgttc atttgaagga agaagaagat tggcagtttg atttagaaca gctagaagcc
    15601 ttagtcaaac ctgaaacaaa aatcatctgt ttgaatagtg caaacaatcc tacaggcaca
    15661 ttgctagatc ggaaaacttt agaaaaaatc gcagaaattg ctcgtacagt agatgcatat
    15721 gtattgatag atgaagtata tgcgccttta acggataaag gtgaattctt gtcgatcgta
    15781 gatgtctatg aaaaaggtat agcaaccaat tccctatcca aaacctactc cattccaggt
    15841 attcgtattg gctggacagc tactggacct gagttggcag aagtttttcg aaagtatcga
    15901 gactacacga tgatttgcgg cggagtcctg tcagatgatc ttgctgtaca tgcattgaag
    15961 aataaagaaa aaatattggc gcgaaacaaa aagatcatta cagagaacct tgtgatttta
    16021 aaacaatggg tagccaatga accgaaagtt gaacttgtcg ctcccaatta cgtctctact
    16081 tctttcatta aattagccat cgaagaagat gaccagacat tttgtatcaa tttattagaa
    16141 gaaacaggag tactattagt accaggaagt gcctttgatt taccgaagca tgcccgacta
    16201 ggttactgtt gtaaaaaaga aaccttggaa aagggattat tcctcttatc tgagttcttg
    16261 aaaaaacagg cctaaacaaa aacactgcaa ggacagtctg tgacaactgt tcaagcagtg
    16321 tttttcattt acgcttattg tctatgagaa ggagagtgcc ctgaagctat ggagactcct
    16381 gaagcttttt cttctgcagt ttcttgctga tggatctctt gtgtattgtg tcgtacaagc
    16441 aagaagttga ccaaaacaag aagagaagtc gaaagaaaga cgccgcgata accaaatgca
    16501 ctggaaacac tggagccgat cataggccct actacgttac cagttgcttg gaaagattgg
    16561 ttataactga agatccgtcc agcagcttga tgaggcgaat attttgtgat caatgcttgg
    16621 acagcaggga gcagacaagc atcagagata ccaatcaaaa aacgtaaggc agctaattgc
    16681 caaacattcg tgacgaatgc cattgggata taaaccagaa tcgcaaaacc caaaccaatc
    16741 gcaagtattc tttcactccc aatcttgtca cctaagcgac caaatcttgg agcggctatc
    16801 aaagtggcaa tcccaggtat cgaagcaatg attccgctga tcaacgtaac attcccatgt
    16861 ccatgcagca attggcgaat gtacagactg atgattggac taatggaatt gttggatgct
    16921 tggatgatca tcgttgtgac gaacataccg atgacgacgt gtggatattt caagtctttg
    16981 aaaatctgtt tggcagaaac catgttttct tttttcactg ggacaaattt ttcatggacg
    17041 aaaaacagac tcaacaagaa gacgaaaaat aagatgatcc ctgtaatgaa aaaggtaggg
    17101 cgatagccaa acgctgaagc agagatcccg ccaagaagcg gtccaagtaa tgttcctgta
    17161 acagaaccag ttgctaaggt tccaagaact tgaccacttt tttcttttgg cgtcccagtt
    17221 gcgacaaggg cagtggcatt actgatataa ccagaaaaaa tcccttgaag cagacgcaat
    17281 gcgactaatt gatacacact agtcacgcaa cccatcaaag aaatgacgat tgccattcct
    17341 aatgaagcac gcaacagcat caattttctt cctttttggt cggctaattt tccccaccaa
    17401 ggagagataa gcgtagtcac caagaaagta gatgaaaaag tcaatccgct ccaaaagttc
    17461 aattgtgacg tactataatt acctaacgta tctatgtaaa gtgacataaa aggcatgact
    17521 aaactaaatc cgataccagc cataaatgtt ccaaaccata agacaaacaa gttttgttcc
    17581 caagtttctc ttttcctaaa taatttttcc tttattcttg cagccaatta gactccttct
    17641 ttctgaaaat tttcatctac agctctttat tataaactac aatatgtagt ttatcaatga
    17701 aagtctcttt tttctctttc tttttcgtgg taaactgaaa gagtgaagga tttattttat
    17761 gagaggaaac atggaaatga aaaaaagaaa tctgtctcaa gagaaaatca tcgactgctt
    17821 tcgagaacta gcagaagaga tggatgttca gcaaataacc tttcagcatt tagccaaacg
    17881 cttggatatc aaggctccct cgctgtataa ccatttcaaa aatattcgtg atgtcaaaac
    17941 agcgctaaca gcgaaacttc tgcaggaact taatgaccag ctgcgacggg ctcttgtggg
    18001 aaaaagtggt caggaagcac tgcagatcta tgcagaaact tatcgatcct ttgctttttc
    18061 aaatcaagca gtgtatgagt tactgatcag tgtaccgcat accaatgaag ctgttttatt
    18121 agatggaatc catgaaacga atcagatcat tttgcagata tttgaggctt ttcaattaac
    18181 gaaagaagaa aaagttcatc gaagcaggga acttcgtagt atgatccacg gttatctttc
    18241 cttacgtttt ttaggatact ttacaaaaga accaacaatc gatccagaag acagctattt
    18301 ttggatgatc aataacttta tcgctacttt gccttaaaca atcagattca tttgtaacaa
    18361 ttttaagatg ttcattaaat gaccctttta taagagaaaa ttcttgcaac tttcttgaaa
    18421 agtgactaag atgagtctag aaaagctgaa agaaggagca cgagtatgta tcaagtaatt
    18481 accatgttcg gggacaatga gccttggtgg ttttttgaag actgggaaga agatattgaa
    18541 gaagaagaaa cattcgagtc tttcgaagaa gcacgacaag cgtacgaaaa acaatggaac
    18601 gaaatccatc aaaattatga atatatcaat gcaaaaagta actttctcag cgccttttgg
    18661 aatgacggag atgagcgctg gtgtgaagag tgtgacgatg atttacagca atacaaggga
    18721 cttgcgttgt tgaaaaatca tcagcccatc acagtagaaa gcagaaagga attttatgaa
    18781 acaactaatt ctagcggaaa aaccaagcgt tgcgaaagac ctaagcaagg tgcttggtgc
    18841 taatcaaaag cacaaaaatt attatgaagg acccaaggtg atcgttactt gggcccttgg
    18901 tcatttatta ggattgaaaa tgccggaaga tctgaataag gaatggcaga gctggcaaat
    18961 ggacacgttg ccaatgatcc ctaaaaaact aggaatcaaa ccacttccta aaacaggaca
    19021 tcaattaaaa gcaatcaagc agctggctca gcgaaaagat gtctcagaag cagtcattgc
    19081 gacagatgcc ggaagagaag gggaacttgt tgctcgctgg atcttagagt atgtgcattt
    19141 caataaacca gtgaagcggt tatggatctc ttctcaaaca gataaagcaa tcaaaactgg
    19201 attcaagcaa ctaagacctg cgaaagaata tgatgcattg tatgaatctg ctcttgcccg
    19261 ggcaaaagcg gactggctgg taggattgaa tgtgaccaga gccttaactg tgaagtacgc
    19321 ggataattta tcagcaggcc gtgtgcagac accgacacta gcattagtga gagaccaaga
    19381 aaagaaaatc gagcagttcc gtccacaaac gtatttcacg atcacgttgc aagctggaaa
    19441 agaacaagcc aaaatgcttc aaaaaaatca atttgcttta aaaacacaag aagaagcaca
    19501 aaaacttgtt caattgatca gtacaagcaa aggaactgtc acagaagtcc aagaaaaatt
    19561 aaaaacggaa tctgcgccat tgccttatga tttaacagag atccaaagag aagccaatca
    19621 acgctatggc tattctgcaa agaaaacatt aagtcttgta caaagtttgt atgagaccca
    19681 taagatcgtt acttatccgc gtacagacag caaatatctg acaaatgaca tgaaaagtac
    19741 gatgaaagaa cgattgcaag cagtcagcga ctttgctcct gaggtcaagc agtatttgaa
    19801 aaacggagcc atcgtccaac agacaaaagt attccaagac agtaaagtga ccgatcacca
    19861 tgcacttttg ccaactgaaa acagagcacg ttatgaaaaa ttaagtaatg aagagcgaaa
    19921 aatctatcaa atgattgtct ctcgtttttt aggactgttt gctcaacccc ataaagtcag
    19981 ccaaacaaaa gttacggttg aatttggtaa agaacgattc atttttcggc aaaatcgtgt
    20041 cgtccaagca ggatggaaag gcgaaactga aactgagacg gaaacagtca aatgggaaaa
    20101 aggaatgaga atcaatcccg atttcgccat caaaaaagaa ctatctgcac cgcctaaacc
    20161 attgtcagaa gcaagcttgc ttggtcaaat ggaaaaatac agtctgggaa ccccagctac
    20221 gcgtgcagag attatcgaga aactaatcaa atctgaactg atggaacgta cccctcaagg
    20281 actgcaagta tctcctaaag gtaagcaatt attagtattg gtaaatccct ctttagtaac
    20341 accagatttg acagaaagct gggaaaaaga ccttgaagct attgctgcag gaaagaaaca
    20401 agcaggaacc ttcttgaatg gaatcgaaaa agaaacaaaa cgcttggtga atgagatcaa
    20461 atcaagcaaa caagaatatc aagatttctc gattacccaa aagaaatgcc cagaatgtgg
    20521 ggccaatctg cgcgagaaaa acacacgtga cggcaaaatc tatgtctgca ccaattcaga
    20581 ttgcaactac cgcagaagaa aagatccaaa agtctctaat catcgatgcc cgcaatgtca
    20641 ccggaaaatg gaaatcatcg aaggtaaaaa cggtgcttat ttccgttgta aatacgatgg
    20701 aacgacagaa aaattgcttg ataaaaaaga gcagaagaag aaaatgacca aacatgaaga
    20761 acgacgcttg ctgaaaaaat atgcacaagc agaagaacca gaagaaagcc cattagcagc
    20821 agcactaaaa gcagcaatga agaagtaata agatatgaaa gaggttgtga tagaagtggt
    20881 cagcttcaag aaataagaag gaatttacga aaattgcttc tcaaattttt gtgaaatttc
    20941 ggcttatttc cgaagaagct acttctggaa caccatttat tcggaaaaaa ggactgtgac
    21001 aagacttttg tcacagtcct ttttcattta aaaaaatgtt tacttcattt gacgtatatt
    21061 attggtgagg cttcaattca tttggcttca ctttttctgt ttctcttatg atataaacag
    21121 gacgctgttt cgtttctaag aagattttcc caatatattt cccgatgatt ccaagacata
    21181 acaattggat tccaccaatg aacaggaaga tcgagaccat ggaaggccat cctgatgtag
    21241 gatcaccaaa caacaatgcc cgcaaaataa tgaacaaaat tgcaatacca gaaccgacac
    21301 aagacaaagc gccgataaat gaagcaatcg ttaacggtgc atccgagaag ttcacgatac
    21361 catcaatcga gtagttgaat aatttccaga aggaccaaga tgtttcgcca gccacacgtt
    21421 cgcggttttc ataagaaata tattctgttt taaatccgac ccagctaaat aatccttttg
    21481 aaaatcggtt gtattctgtt aattctagga cggcatccac catttgacga gtcatcaagc
    21541 ggaaatcacg agcgccatcg accatttctg tatcgctgat tttattgatc aactggtaga
    21601 attttttcgc aaagaaactg cgtatcgcag gctcgccttc acgagtcgaa cgccgggtgc
    21661 cgacacaatc gatatcagaa gtagtgatcc ggttgatcat ctcaggaagc agttcgggcg
    21721 gatcttgcag atcaacatcc atcacggtca ctaaatctcc tgtagaagct tgcaatccag
    21781 cgtacagagc cgcttctttc ccaaaatttc tggaaaatga gacaaagcgg acataaggat
    21841 aagctgaggc tagcttcttg attgctgcta acgtaccgtc ttttgatccg tcgtcaataa
    21901 acaaatattc gaactcatgg gcacctttga atttttcaac ctcatcaaaa aaaagaggaa
    21961 cagttttttc ttcattaaaa catgggacaa caattgatac tttcatcgtt tctatccttt
    22021 caatcgtatg tttatatatg gtttacattc aaaatttaat aaaccttaaa taacaccttt
    22081 aaaatttgat tttacgtaaa agaacaggta ttctttttgt aaactaacgt atttagttta
    22141 ccatgtttcc tcggtttatc aaacttttaa gaacaatttt tattagttgg caagtagttt
    22201 tggatagaga aaaaggagga agttccagtc tattgactag gagcaaacaa aatgaaaata
    22261 cacacaccta tcggcttttt atggttagat gcagatgaat ccggattgac tgctgtatct
    22321 tttcagccaa tttcagaaag cgaatcagcg aatcagcgaa ttttaacgcg cacacaagaa
    22381 cagtttgaag aatattttgc gggaaaaaga aaagaatttg atgttccttt atctataaag
    22441 agaggtacgc cttttcagca aaatgtctgg aaagctttgt cgaccattcc ttatggagaa
    22501 atccgaacgt ataaagaagt agccgttgct gttgatagtc cgaaagctgt gcgagcgatt
    22561 gggcaggcaa atcgaaccaa tccattgcca atcgttattc cttgtcatcg agtgatcggg
    22621 caaaatggaa aactgacagg atatatggga aacgcaatag atggggttga aatcaaacgc
    22681 caattgttgt tgttagaagg atatctgcaa aattaaaaac agttgtgatt cggacattgt
    22741 tccatgcgag aatcacaact gttttttcat gttcattcta aactttgatc agccaaataa
    22801 atagtaaagc catcactgtt tggagaagcc cgacagctaa tccataagat aagaagcgtg
    22861 taccttcttt catgaatttt gcaaagtcta aacgcagacc aattgccgct aaagcagtga
    22921 tctcaaacca gctgctgata aaatgtgccg attggtcaat aactacaggt aaagaaaaca
    22981 agctgttgac caaacaaaag atcacaaacc ctaatacata ccaaggaagc ggtaattttc
    23041 gttttttctg cgtatcagtt ggcgtttcaa acagagctgg ttgtttcttg tgcatgaatt
    23101 tttcaaacag atagacaaca acaacgagca tcataatacg tagaatctta aatagcatgg
    23161 cgaattcgac aacagaattg tcaagtaagc ttgccccagc gactacttga ccaacagatt
    23221 ggagtgtacc acccaaaagt gcactttttt caatgagatt tccaccaaac aactgtaatc
    23281 caagaaaagg gagagtcaac atcatcacag tacccagtag attgactagt gtgatgatct
    23341 gtcctttttc ttcctctttc gcatggatgg aaggggcgat tgaagcaata gcagaagaac
    23401 cacaaacagc gtttcctcca gccatcatca aagaagaagt atcgctgaat tgcagttttt
    23461 taccaatcaa gtaagcaccg atgatagtga ctgacatttg cagtaacaca aaaacaattc
    23521 cttttacacc aatttgagca atcgtttgag ctgtgacagt tgcacctagt aaaaaaacag
    23581 agaattctaa tagtttgctt tcagcaaatt ttgtgccgat tgctagaaca ggctgtttaa
    23641 aaaaagtatt tcctaataaa atacccaata aaatagcaat agtcgctcca cctaactgtg
    23701 gcagataaat agcaagaagc ctgctgaagg cagcgatggc aaaagaaagc agtaaaccgg
    23761 gtaagatttc gcggaccgct gaatccttta cagattttat tggatgtgac aatgatttca
    23821 tttgatcggt tcctttcgtc aataaatact aaaagcatac tcgaacatag tagcactaaa
    23881 ccagtatttt gacaaaagaa caagaggtcg tgacaaaagt cttgtacgac ctcttgtttc
    23941 gaata
//
LOCUS       DAYYAR010000030        23829 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000030 DAYYAR010000000
VERSION     DAYYAR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.030"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACHAT0_001046"
     CDS             complement(54..788)
                     /locus_tag="ACHAT0_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8451004.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACHAT0_001047"
     CDS             1115..1525
                     /locus_tag="ACHAT0_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8451005.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACHAT0_001048"
     CDS             1518..2219
                     /locus_tag="ACHAT0_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8451006.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACHAT0_001049"
     CDS             2219..3805
                     /locus_tag="ACHAT0_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451007.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACHAT0_001050"
     CDS             3824..4045
                     /locus_tag="ACHAT0_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8451008.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACHAT0_001051"
     CDS             4042..4803
                     /locus_tag="ACHAT0_001051"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8451009.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACHAT0_001052"
     CDS             5183..5692
                     /locus_tag="ACHAT0_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8451010.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACHAT0_001053"
     CDS             complement(5767..6306)
                     /locus_tag="ACHAT0_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8451011.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACHAT0_001054"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACHAT0_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8451012.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACHAT0_001055"
     CDS             7319..8170
                     /locus_tag="ACHAT0_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8451013.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACHAT0_001056"
     CDS             8198..8833
                     /locus_tag="ACHAT0_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8451014.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACHAT0_001057"
     CDS             8853..9626
                     /locus_tag="ACHAT0_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8451015.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACHAT0_001058"
     CDS             10034..10831
                     /locus_tag="ACHAT0_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8451016.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACHAT0_001059"
     CDS             10809..11222
                     /locus_tag="ACHAT0_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8451017.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACHAT0_001060"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACHAT0_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8451018.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACHAT0_001061"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACHAT0_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8451019.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACHAT0_001062"
     CDS             15999..16559
                     /locus_tag="ACHAT0_001062"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8451020.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACHAT0_001063"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACHAT0_001063"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8451021.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACHAT0_001064"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACHAT0_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8451022.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACHAT0_001065"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACHAT0_001065"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8451023.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHAT0_001066"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHAT0_001066"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8451024.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHAT0_001067"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHAT0_001067"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8451025.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACHAT0_001068"
     CDS             21907..22485
                     /locus_tag="ACHAT0_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8451026.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACHAT0_001069"
     CDS             22537..23019
                     /locus_tag="ACHAT0_001069"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8451027.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACHAT0_001070"
     CDS             23136..23510
                     /locus_tag="ACHAT0_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451028.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23623..>23829)
                     /locus_tag="ACHAT0_001071"
     CDS             complement(23623..>23829)
                     /locus_tag="ACHAT0_001071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451029.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7781 a         4286 c         5115 g         6647 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact cctttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagtt
//
LOCUS       DAYYAR010000031        23792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000031 DAYYAR010000000
VERSION     DAYYAR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.031"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..993
                     /locus_tag="ACHAT0_001072"
     CDS             136..993
                     /locus_tag="ACHAT0_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8451030.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1108..2028
                     /gene="pstC"
                     /locus_tag="ACHAT0_001073"
     CDS             1108..2028
                     /gene="pstC"
                     /locus_tag="ACHAT0_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8451031.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2028..2912
                     /gene="pstA"
                     /locus_tag="ACHAT0_001074"
     CDS             2028..2912
                     /gene="pstA"
                     /locus_tag="ACHAT0_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8451032.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2927..3733
                     /gene="pstB"
                     /locus_tag="ACHAT0_001075"
     CDS             2927..3733
                     /gene="pstB"
                     /locus_tag="ACHAT0_001075"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8451033.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3749..4507
                     /gene="pstB"
                     /locus_tag="ACHAT0_001076"
     CDS             3749..4507
                     /gene="pstB"
                     /locus_tag="ACHAT0_001076"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8451034.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4520..5197
                     /gene="phoU"
                     /locus_tag="ACHAT0_001077"
     CDS             4520..5197
                     /gene="phoU"
                     /locus_tag="ACHAT0_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8451035.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5435..5734
                     /locus_tag="ACHAT0_001078"
     CDS             5435..5734
                     /locus_tag="ACHAT0_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451036.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5775..6467
                     /locus_tag="ACHAT0_001079"
     CDS             5775..6467
                     /locus_tag="ACHAT0_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8451037.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6464..7231
                     /locus_tag="ACHAT0_001080"
     CDS             6464..7231
                     /locus_tag="ACHAT0_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8451038.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7331..8899
                     /gene="liaX"
                     /locus_tag="ACHAT0_001081"
     CDS             7331..8899
                     /gene="liaX"
                     /locus_tag="ACHAT0_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8451039.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9196..9516
                     /locus_tag="ACHAT0_001082"
     CDS             9196..9516
                     /locus_tag="ACHAT0_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8451040.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9516..9866
                     /locus_tag="ACHAT0_001083"
     CDS             9516..9866
                     /locus_tag="ACHAT0_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8451041.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10033..10971
                     /gene="hprK"
                     /locus_tag="ACHAT0_001084"
     CDS             10033..10971
                     /gene="hprK"
                     /locus_tag="ACHAT0_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8451042.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10986..11816
                     /gene="lgt"
                     /locus_tag="ACHAT0_001085"
     CDS             10986..11816
                     /gene="lgt"
                     /locus_tag="ACHAT0_001085"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8451043.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11860..12885
                     /locus_tag="ACHAT0_001086"
     CDS             11860..12885
                     /locus_tag="ACHAT0_001086"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8451044.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12900..13838
                     /gene="galU"
                     /locus_tag="ACHAT0_001087"
     CDS             12900..13838
                     /gene="galU"
                     /locus_tag="ACHAT0_001087"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8451045.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13963..14403
                     /locus_tag="ACHAT0_001088"
     CDS             13963..14403
                     /locus_tag="ACHAT0_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8451046.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14406..15008
                     /locus_tag="ACHAT0_001089"
     CDS             14406..15008
                     /locus_tag="ACHAT0_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8451047.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15076..16182)
                     /locus_tag="ACHAT0_001090"
     CDS             complement(15076..16182)
                     /locus_tag="ACHAT0_001090"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8451048.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16392..17393
                     /gene="ccpA"
                     /locus_tag="ACHAT0_001091"
     CDS             16392..17393
                     /gene="ccpA"
                     /locus_tag="ACHAT0_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8451049.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17729..20074)
                     /locus_tag="ACHAT0_001092"
     CDS             complement(17729..20074)
                     /locus_tag="ACHAT0_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8451050.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20353..21255
                     /locus_tag="ACHAT0_001093"
     CDS             20353..21255
                     /locus_tag="ACHAT0_001093"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8451051.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21468..23243
                     /locus_tag="ACHAT0_001094"
     CDS             21468..23243
                     /locus_tag="ACHAT0_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8451052.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23500..>23792
                     /locus_tag="ACHAT0_001095"
     CDS             23500..>23792
                     /locus_tag="ACHAT0_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8451053.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKT"
BASE COUNT         7588 a         4226 c         5012 g         6966 t
ORIGIN      
        1 gcatagaaac ttttaatgag atagtttttt ttacaattat gagaatttat tttgttattt
       61 aatgattttg attcgttttc atcaagtaat gaaaatgata aaataaacat actttaggat
      121 taaaggagat ttctaatgaa aaaacttaaa ttattcttac caataatagg attagccatt
      181 cttttatcag gctgttcaaa gtggattgat cgaggagaat ccattacggc tgttggctct
      241 tctgctttac agcctttagt agaaacagtt gctgaaactt accagacaga aaatcctgga
      301 aaattcatca atgtacaagg gggcggaagc ggaactggtt tgtctcaagt acagtcagga
      361 gcagtcgaca tcggaaactc tgatttgttt gcggaagaaa aatcggggat cgatgcctca
      421 gcattagtcg atcatcgagt agcagttgtt gggatcactc ctattgtgaa caaggatatt
      481 ggcgtaaaag acatcagtat ggaaaacctg aaaaaactat ttacaggcga gatcaaaaac
      541 tggagtgaag tcggtggaaa agatcagcca gtagttatat tgaaccgcgc ttcaggaagc
      601 ggaacccgta gcacgtttga aaaatgggta ttggacggag aaacagctgt tcaggcacaa
      661 gaacaagatt caagcggaat ggtgcgccag atcgttgccg acacacccgg tgcaatcagc
      721 tatgttgctt tttcttatgt ttccgatgat gtagatacgt tgaatgttaa cggtgtagaa
      781 ccgacagatg aaaatgtgac aacgaacaaa tggacgattt gggcatatga gcatatgtac
      841 acaagagatc atccaaaaaa actgacaaaa gaatttttag actatatctt atctgatgaa
      901 gtacaagata acatcgttgg agaactaggt tatatccctg tttctcatat gaaagtcgaa
      961 cgtgattggc aaggaaatat cgttaaagag taatctttac acaaaattta caaacttagc
     1021 taaactttta catgttatta aaaccaaatt gatgttcaat ttgtacactg agtgagtatg
     1081 ttaaacctga ttaaggggga gtcatctgtg gaagatgtga aaaaagtatt attaacaaaa
     1141 tcaaaacgtt caaaaatgga acagcgtgga aaattcatca gttttttatg tatcgcgctg
     1201 atcgtgttgg ttgtattatc tattttttat tttgtagcta gtaaaggatt ggccaccttt
     1261 tttactgata agatcaatgt atttgatttt ctgtttggta cagattggaa tccaagccaa
     1321 atcggagcaa atgggaaacc aatggtaggg gcattgccta tgatcacagg ttcgtttatt
     1381 gtgacctttt tatctgctat cgttgccacg ccatttgcca ttggtgcagc agtattcatg
     1441 acggagatct ctcctaaaaa cggaacgaag atcttacagc cggtcatcga gctgcttgta
     1501 ggtattcctt ctgtcgttta tggttttatc ggtttatcag ttatcgtgcc ttttgtcaga
     1561 agtatttttg gcgggacagg gtttggtatc ttagctggta catttgtgtt gttcgttatg
     1621 atcttgccga ctgtcacaac gatgactgtt gatgcattaa aggcagtgcc acggcactat
     1681 cgggaagctt cattagcgct tggtgccaca agatggcaaa caatctacaa agtcgtgcta
     1741 cgagcagccg tgcccgggat tttaacagca gtcgtttttg gtatggctcg tgcgtttggt
     1801 gaagccttag ctatccaaat ggtcatcggt aatgcagcat tgatgccaac aggacttact
     1861 actcctgctt ctacattgac gtccatctta acaatgggaa tcggaaatac aatcatggga
     1921 acagttgaaa ataatgtgtt atggtcatta gcgctgatct tgctattgat gtcattattc
     1981 tttaatatcg tgatccgtat gattgggaag aaaggagagc tgaaataatg aacgcaaaac
     2041 gctcagataa aattgctaca ggtgttttat atatagtttc ggggatcatc gtattgattt
     2101 tagcagcttt actactttat atcttagttc ggggaatacc gcatatttca tggtccttct
     2161 tgaccgaacc ttccaaagcc taccaagaag gcggtggaat cgggatccag ctatttaact
     2221 cgatttattt gcttttgatc acgatgatca tcagttttcc tatttcttta ggtgcgggta
     2281 tctatctttc agaatatgct ggaaaaaact ggctaacaga tgtgattcgc acgtcgattg
     2341 aaatcttgag ctcgttgcct tctgtcgttg tcggtttatt cggtttcttg gttttcgtta
     2401 ttcaattcca gtatggtttc tcgattattt ctggtgcatt ggctttgact gtattcaact
     2461 tgcctctttt gactagaaca gttgaggagt ctttacaagc ggtccattac acacaacgag
     2521 aagcaggatt ggccttaggt ttgtcacgct gggaaacagt gataaaagtg gtggttccag
     2581 aagcgacacc agggattttg actggtgtga tcctaagttc tggaagaatt tttggtgaag
     2641 cagctgcatt gatctatacg gctggacaaa gtgcacctgc gttagatttt acgaactgga
     2701 atccattaag tgtttcaagc ccgatcagca ttttccgtca agctgaaaca ttagctgtcc
     2761 atatttggaa gatcaataca gaaggcacga tgcctgatgg tgtttctgtt tcggctggcg
     2821 cttctgccgt gttgatcatt gctgttttac tgttcaactt cggcgctcgt gcgattggca
     2881 aacgattata ccgaaaaatg acttcagcct aaggagaaga agcaaaatga aaaaatataa
     2941 ctggaatgaa acaaatgtca tcaccatgcc aaaagagaaa gaagtagcac tatatacgga
     3001 tgatctacat gtctggtatg gcgacaatga agcaatcaaa ggagtggatc tggaatttga
     3061 aaaaaataag atcactgcac tgattggtcc ttccggctgc ggaaaatcta cgtatcttcg
     3121 ttcgttgaat cgaatgaatg atgggatcgc taatacgaaa atcactggga aaatcatgta
     3181 tcaaggcgta gatattaatg cgcctcaagt ggatgtttat gagatgcgca aacggattgg
     3241 catggttttt caacgtccta atccgttcag caagtcgatt tatgacaata ttacatttgc
     3301 tttgaaacag cacggcgaaa aaaacaaaaa gaaattagat gaaatcgttg aaacaagctt
     3361 gaaacaagct gccttgtggg accaagtaaa agacagcttg gacaaaagtg ccttggcttt
     3421 atctggcggg caacagcaac gtttatgtat tgctcgtgcg atcgcgatga aacctgatat
     3481 cttgctgcta gacgagccgg caagtgcttt ggaccctatt tcgacaggaa cggttgaaga
     3541 aacacttgta aacttgaaag aagactatac gatcatcatc gtgacgcata acatgcaaca
     3601 agcagctcgt atcagcgact atactgcctt tttctatcta ggaaaagtaa tggaatatga
     3661 caaaactaac aagatattta ccagaccaaa aattcaggca accgaagatt atgtgtccgg
     3721 tcattttggt taagacggag gcctcaacat gacaaaagaa attatttcat ctaaagatct
     3781 tcatctgtat tatggaaaga aagaagcact aaaagggatc aatctaagtt tcaatcaagg
     3841 ggaaatcact gcgatgatcg gtccttctgg ttgcgggaaa tccacttatc tgcgttcttt
     3901 gaaccggatg aatgacctga ttccgaatgt aacgatcacc ggaagtgttt tatataaaga
     3961 caaagacatc tatggtccaa aaacagatgt tgttgaacta agaaaagaaa tcggcatggt
     4021 attccagcag ccaaacccat ttcctttttc tatctatgaa aatgtcatct atggattgaa
     4081 attaaaaggg gaaaaagata aaaaagtcct tgatcaagta gttgaagaaa gcctgaaggc
     4141 agcgtctgtt tgggatgatg tgaaagacaa attgcataaa agtgctttat ctttatccgg
     4201 tgggcagcag cagcgtgtat gtatcgcacg tgttttggca gttaatccgg aaatcatctt
     4261 attggatgaa ccaacaagtg cgcttgatcc tgtttctact gggaaaatcg aaagtatgct
     4321 gttagaatta agagagcggt atacgatgat catcgtgacc cataatatgt ctcaagcttc
     4381 acgtatttca gataagacgg cattcttttt agatggtcac ttgatcgaat acaacgatac
     4441 gaagaagata ttcatgaatc cagaaaaaca agagacagaa gattatattt caggtcgatt
     4501 tggataagga ggaaacggaa tgctacgtac acaatttgaa gaagaattgc tgaatctcca
     4561 taatcagttc tacgaaatgg ggatgatggt gagcagcgcc gtgcacaagt cagttcgtgc
     4621 atacatcaaa cacgataaaa tcttagccca agaagttatc gaaaacgata taaatatcaa
     4681 taacatggaa acacgcttgg agaagaaaag ttttgaaatg atcgcattgc aacagccagt
     4741 tacaactgat ttgcgtatga tcattacggt catgaaagct agctcggatt tagaacgtat
     4801 ggccgatcat gcggtgtctg ttgctaaatc cacgattcgc gtaaaagggc agacgagaat
     4861 accggaaatc gaaaaagaga tctccgatat gtccgattat gtgaaaaaaa tggtggacaa
     4921 cgtcctagtt gcttatgtca aaacagacga aaaagacgca cgtacgatcg ccaatatgga
     4981 tcaaagagtc aatgactatt tcaatcgaat ctataatgca acaatcaaaa atatgcagga
     5041 aaatcctgag acagtcatca gtggaacaga ctacttgaat gttgccagtt atctagaacg
     5101 gatcggagac tatgtgacaa acatctgtga atggatcgtc tacttggcta cagggaaaat
     5161 cactgagtta aatacgaacc acaatgagat tttctaaact gaagctgtga ttatgccgtt
     5221 tgtttaggaa aagaggttgt gaaatcagcg gttgtgaaaa agctgttctg catcaagtaa
     5281 taagaacagt caaacaaaaa atagctcctc atatttttcg tttggctgga cttattaccg
     5341 cagatgcaag cttttgaaca ccgtttattc gggaaagggt ttgtgacaaa acatgtcaca
     5401 aacccttttc atcatttcat agaaaggata aagaatgcaa aaaaaaatta gcatcatcac
     5461 tatatttttt atttgtgtgc ttacttcatg ggttattcag catcaagctg ctttatatga
     5521 agtacctgtc atcgagatcg aaaaagtacg aataaaagag gatcatcaat tgattacagg
     5581 aagactgatg aacacggatc aaaaaggaga atcgatttat ttatcggttc cttatcaaaa
     5641 aaatcaaata gaaacagcaa atataaaagc gagacaaatg tatctagtcc aaatgcatgg
     5701 agatgaatgg cagctagtag caaaaaaaaa atgatggctg gcttttcttc ttcacaagta
     5761 tctccattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct ttaggaagtc
     5821 tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa acagaaggga
     5881 tttctctatt aaatatatct atcctgttcg tcgttatagg gatgattatt agcatattgg
     5941 ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt acgagtgttt
     6001 ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa ggactgcgct
     6061 atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct agcgtactga
     6121 ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca ttagaagaat
     6181 tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga aggcaagtgg
     6241 gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc agtgggtcag
     6301 ttccatcgct tcttttatat ttaggaaatc aatggtcgtt caaagaaacg atagaacagc
     6361 atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta ttatcgatcc
     6421 ctatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg tacttattat
     6481 acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag gtatgtatat
     6541 tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg gttatcatca
     6601 gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg tggttatctt
     6661 attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta tactgatctt
     6721 tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc aaggttttac
     6781 atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg attttagatt
     6841 acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg ctagtgtagc
     6901 aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt tttaccaatt
     6961 gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa tgacacttct
     7021 ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt tagacattcc
     7081 ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg ctattcttac
     7141 cacattgtct gcactacttg ttcttccttt aacctgcgtg actacagcat atctctttac
     7201 aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct atgacatttt
     7261 tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt tgcttaagga
     7321 ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag gaatcctttc
     7381 gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg aaaagcaaat
     7441 aaaaaaagaa gcagacaagg taactgctag tcataaagaa aaagatcagg caagtcaatt
     7501 gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc caaaggaaag
     7561 agaaaggcaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa ctgaagcgaa
     7621 taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata aggcagcacg
     7681 caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag gcgagttgac
     7741 agaagaagaa ttagcacagc gtcagacgtt agaagcagaa atcaaagaat tagaagctag
     7801 tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag atatccgtaa
     7861 gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc cagatgattg
     7921 gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag aagctggatc
     7981 acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag tgaatgacaa
     8041 catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga aatttgagca
     8101 tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag caaacggaaa
     8161 cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga tcaaacttta
     8221 cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga ttgaggttaa
     8281 tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc ttgttttcta
     8341 cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca acatcatgat
     8401 tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca tcatcgtgga
     8461 ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg atattcgaaa
     8521 cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat ttggtgcgac
     8581 agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta cgtcttacga ttaaagaaga
     8641 taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg ctttaccaga
     8701 tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaaca gccgtctgtc
     8761 caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc aattccgtcg
     8821 tgtttcagat ggtgaaatca cgcaagtgca actttcaacg acaacaggaa gtatctattt
     8881 aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag caataagaag
     8941 cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc gataggctat
     9001 ctcttgaaca ccgtttacca agagattatg aacctgccaa ttatcgccga gtcacaagga
     9061 ataatttcag aaaaagctcg tcacgtttta tgaatatggt aatttgtgac cgagaatttt
     9121 gcagaaagac accgattaag aagaaggttg tcacaacgat tttcttcatt taaaatagta
     9181 gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ccaataaaaa tgttgttttg
     9241 acagggactt tagcaggaat aggagaatac atcgggattg atccgactgt tatccgtgtg
     9301 ctttatgtat tcctgagctt agttgctttt ggaagtccaa tcattttata cattttatta
     9361 gctttgatta tcccatctgc gcgttctgca cgaacaaact atggacataa caatgcatat
     9421 tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa caaaaaaacg caaagaagcg
     9481 gaaaaaatcg atgatgacga atggagtgac ttttaatgtc gtactttcaa cgtattttag
     9541 ttaatacgtt aacattcata tcattggctg ttctttttcc tgcgatggtc catgtgagca
     9601 gtatttggac ggccgttctt gccagcttcg tattgtctgt gttgaatatg tttgtcaaac
     9661 cgattttgac gatactgtcc ctgcccttta cattagtcac attgggattg tttacgtttg
     9721 ttatcaatgc agcgattctt aaaatgacct ccttttttgt gggagcagaa cattttagtt
     9781 tttcttcatt cggtgcagcg ttgttagtag ctatcttgat gtcgcttgtc aatgtaattg
     9841 tcagtgaaca taacagtaga aaataaaaga aagctgtgcc gaactatcgg cgcagttttt
     9901 ttatttagaa tcaggaatgc gttttctatt acattggtaa ttaaataaaa acgttttttt
     9961 taattggtag tacttttggc tagacaatga taaaatgata gagaacactc taatagaaat
    10021 gaggtagcat gaatgtcaga agttgtaaaa gttagccaat tagtgaaaaa attatctttg
    10081 gaaatcgtga caggcgacga gaaaagctta gaccgtgtca tcgtaactgg ggacatctca
    10141 cgcccaggac tagagttgac tggttatttt aattattatt cccataatcg tttacagcta
    10201 tttggcagta aagaaatcag ttttgcggaa cggatgatcc cagaagagcg tttgatcatc
    10261 atgagaagat tatgtagtga agatatgccc gcatttatta tttcaagagg attaccggct
    10321 ccagaagaaa tgatccaggc agcaaatgaa aatgggatag ctgtactgcg ttctcccatc
    10381 tcaacttctc gtttattagg agaattatcc agttatttag atggtcgcct tgcccctcga
    10441 acaagtgtcc acggtgtttt agtggatgtt tacggattag gtgtactgat tcaaggtgac
    10501 agtgggatag gaaaaagtga aacagcgttg gagttgatca aacgcggtca tcgattgatc
    10561 gcagacgatc gagtagatgt ttatcagcaa gatgaattaa cggtgatcgg agaaccgcct
    10621 aaaatcctgg aacatctaat tgaaatccga ggaatcggga tcattgatgt aatgaatctt
    10681 tttggtgcga gtgcggtacg tggctctatg caagtccagt tggctgttta tctggaagca
    10741 tgggcaaaag ataagaaata cgaccgtctg ggctctgaag acacgaccgt tgaaatcgcg
    10801 gaagtgggca ttccacaaat aaaaatccct gtcaaaacag gacgaaacgt tgcaatcatc
    10861 attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg gctttgacgc aacgaaaaca
    10921 tttgaagaaa gattaagtcg cttgattgaa gaaaactcag gcaacgacta gcaaggagtg
    10981 acaatatgtt agcacaaatc aatcgagttg cttttgagct tgtagggatt cctatctact
    11041 ggtatgcatt gatcatcgta tcaggtatca ttatcgccat gtggcttagc agcagagagg
    11101 ctgttcgggt aggattaaaa gcagatgatg tgacagattt tatgttgtgg ggattgccta
    11161 tctccattat tggtgctcgc ttatattatg ttttatttga tcttccccag tatatagctg
    11221 atccaatcca aatattcaat atccgttcag gaggcttagc tatttatggc ggactgattg
    11281 caggcgcgat cacgctgtat ttttttacta aatatcattt tatttctttg tggacttttt
    11341 tagatatcgc tgcaccaagt gtattattag cacaggcaat cggtcgttgg ggaaatttca
    11401 tgaaccacga agcatatgga ccagacacga cgcgaagctt cttagaaagt ctgcatttac
    11461 cgcaattcat cattgataat atgtatatcg aagggatcta tcgtcaacca acatttcttt
    11521 atgaatcact ttggagtttg attggattta ttttgctttt gtttttacga aagaaaccac
    11581 accttttgaa aaaaggcgaa gtccttttat gttatctaat gtggtatagt ttcgggcgtt
    11641 tctttattga aggattacgt acagacagtc tttacttttt aggtgtgatc cgtatttctc
    11701 aattattatc tgctgtgtta tttgtcggta cattgattct atttatttgg cgcagaaaaa
    11761 aaggaaattt accggattat gaccgctcat taggaaaaaa tcaacaaatt atctgatttt
    11821 tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa tgaattatag agtagcggtt
    11881 ttaggaccag gctcttgggg tactgctttg gcacaagtcc tcgccgaaaa cggacatgac
    11941 gttcggattt ggggacatcg tgcttcacaa gtagaagaaa tcaacaccca acacacgaat
    12001 caacgttatt taccagatca ttatctaccc gattccatcc aagcattcac caatatggaa
    12061 gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc caacgaaagc aatacgttct
    12121 gttgcaaaag aactagtgcc taaattgaat acaaagccag tgatcatcca tgcaagtaaa
    12181 ggattagaac agggaacaca taaaaggatc tctgacatct tgttagagga aatcccagca
    12241 gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc atgcagaaga agtagcgaaa
    12301 catgacatca ctacgatcac agctgcaagt acagatgcaa aagcagcaaa atatgtacaa
    12361 gagctgttca tgaataatta ttttcggatc tataccaatc ctgatgtgat cggtgtagag
    12421 atgggggcag ctttgaaaaa tattattgct attggtgctg gcgctattca cggcttaggg
    12481 tttggcgata atgcaaaggc agcaatcatg acgcgcggat tagctgagat cagccgttta
    12541 ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat taagcggagt aggcgatcta
    12601 gtcgttactt gcacaagtgt tcattctagg aactggcgcg caggcaactt gttaggacaa
    12661 gggcagcccc tagaagaggt gctagccaat atgggaatgg tcgttgaagg tgtgaatact
    12721 actcaagcag cgatggaact agcccaaata atggaaatcg aaatgcccat cactcaaaca
    12781 atctatgaag tgctgtatga acaaaaagaa atcaaaacag ccgcaaaaga aatcatgtta
    12841 cgtgatggga aaaaagaaaa tgaattctca ttaacaattt tttgaaggag cgaatgtata
    12901 tgaaagtcaa aaaagcagtt attccagcgg caggattagg aacaagattt ttaccagcaa
    12961 caaaagctat ggcaaaggaa atgttgccaa ttgttgataa accaacaatc cagttcatcg
    13021 ttgaagaagc gttgaaatca ggaatcgaag acatcttgat cgttacagga aaagctaaac
    13081 gaccaatcga agatcacttt gattcaaata ttgaattaga aacaaaccta agtgaaaaag
    13141 gaaagaccga attattgaaa ttagtagaag aaacgacaga tgtcaacttg catttcatcc
    13201 gtcaatctca tccaaagggt ttaggacatg ccgttcttca agcaagagcc tttattggaa
    13261 atgaaccgtt tgtcgttatg ctaggcgatg atatcatgga agatgaagta ccattgacac
    13321 agcaattaat cgatgattac gaacgtactc atgcttctac gatcgccgtt atgcaagttc
    13381 ctcatgatga aacttcaaaa tacggaatta tcaatccagg agaagtattg gaagatgggt
    13441 tatacaatgt caaaaacttt gttgaaaaac cagatccaag caaagcacca agtgatttag
    13501 ctattattgg acgttactta ttgactccag aaatttttga tgtattgtcc gagcaacaac
    13561 caggagccgg aaatgaaatc caactaactg acgcaatcga tacattgaat aaaacacaac
    13621 gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt cggtgacaaa tttggtttta
    13681 tgaaaacaag tattgaatat ggtctaacac atccagaagt aggtgcacct ttacgtgagt
    13741 atatcctgtc tttaggtaaa aaattagaag cagaaggaag caagatcgat cataatccaa
    13801 aaggaataac agaaatcaaa gaagaaaaga aaaaataacc cttttttcta acgagcttca
    13861 gctgctaatt gaataaaaag tagttgaagc tcgtttttat ttgtttaaaa aacacgaaaa
    13921 tgctatcctt atagaaaaga gagacaagaa gggagtttga tcatgtcagg aggagaaatt
    13981 gcaggattga tcgcagcaat cgcctttgcg atacttgtcg tttttatcgt tcgtgtgttg
    14041 ttgcaagttt ctaaaacttt agaaaaagta gatcagactg tagcagaagc aaatacgacg
    14101 atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac aagtagaagg cttgctggtc
    14161 aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg caacgatcga tccattgttc
    14221 acagcagttg ctgatttaag tgaaagtgta tcagaactta atcattctag taaaaacatc
    14281 gcgacgaaag tcggcggaat cggtaaaacg acagctaaag cgacggttgc cggaaaagtc
    14341 ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata ctgaaacgac aggaggaaaa
    14401 taaaaatggc taaaaaaggt ggatttttat taggtgcagt agtcggagga acagccgcag
    14461 caatcgccgc gttattgtta gcaccaaaat ctggtaaaga acttcgggat gaattagctg
    14521 cccaagcgga tgatttgatg gatatggctt cagattacac agatatggcg aaagaaaaat
    14581 cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga tctttctcaa caagcaggtg
    14641 atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc agatgaagca ggaagtgaat
    14701 ctgacgatat cttgttaaat cttaaagaac aatcagctga cttatctaat cgtttcaaaa
    14761 aaacggcaga agatatgaaa aaacctgcag atgaattagg aagtattgct aaagatactt
    14821 cagaagatat catgattgat gtaaaagacg ctgctggtaa agcaggagat actgtcaatg
    14881 aaggaatgtc agaagcaaaa ccagttgcag atgacacaaa agaaatgatg aaaatggcaa
    14941 aagaagatgc gaaagaagct aaaaaagaaa taaaagatac aatgacagat tcatctacaa
    15001 atcactaaaa tcactaaaat cacaaattta tagaataaaa aggtgggagc ggactagctc
    15061 tcaccttttt tactcttatt tttctaaaac tagtaattct tttggtgtag tcgtgaatgg
    15121 cacacagcca tcgcttgtca catatacaca gtcttcgata cgtaccccta ctttttcagg
    15181 aatatagatt cctggttcaa gagagaagca catcccttct tcgattacta agtcatttcc
    15241 ttctacaagg gaagggaatt cgtggacggt tgttccgatc ccgtgaccta aacggtgatt
    15301 gaaatattcg ccgtagccat agctgttgat cacgtttcgt gcgatttgat caagttcacc
    15361 tgctgtcaca cctggacgga cggcttcttg tgcggcaagc tgtgcttcaa gagtgatgtt
    15421 gtatattttt tcttggaagt cggttggttt ttgataagca attgtacgag tagcatcact
    15481 gcaatagcca ttccaaacaa cgccaagatc gaataacaca agttcattcg gtgaaatctt
    15541 cgtgtttcct ggtgtaccat gagggcttgc agcatttgct cctgcaagga ctaatgtgtc
    15601 gaaagacatt tgtgaaacac cttgtttttt caattggtat tcgatttctg caatgatcgc
    15661 ttgttcagtc acgccttctt tcactgcttt aaagccaatc tcaaatgcaa catcggccca
    15721 tttaccagct tcgatcagtt tgtctttttc ctctagagtt ttgatcagtt gtagacgttg
    15781 gatgatggaa gctagatcag tgtaagaatc cgttcccgtc aagatatcag agatagcatc
    15841 gaagcgctcg acagtcaagg cgttcttttc aatccctaca ttgccaggct gaccatagcg
    15901 agcacgaagt tcttttccga tgatctccca agggttttcg ctgtctaaat agccacggac
    15961 atcatacgcc caactactgt tttgagcatc ttcaacttcc aaagcaggag tgaaaaggaa
    16021 aggatcttgt tcaagaggga taaaaagagc tagtacacgc tcatgaggat cactttcgta
    16081 tccggaaaag taagcgatgt ggccagggtc gctgatataa gccaattcga cttggtgttg
    16141 tgccatccat tgacgtagtt cattgatttt tacgttattc atttggcaac ctactttcta
    16201 aattaatttt gtattcaata attgtaccac tgatttatta aaagaccaac gttctaaaaa
    16261 ctgaaaaaag taaaaatttt catgaaaaca gagataaaac ggttgcaatt cttgagatta
    16321 tttgatattc tattttagaa atgtaagacg tattttcaga aaacatataa aatatgaata
    16381 ggagaacaca aatggaaaag caaacaatca ctatctatga tgtagcgaga gaggcaaatg
    16441 tttcgatggc tactgtttca cgtgtcgtaa atggcaatcc aaacgtcaaa ccagcaacaa
    16501 gaaaaaaagt cttggaagtt atcgatcgct tggattatcg tccaaacgca gttgcgcgtg
    16561 gcttggcaag taagaaaaca acaacagttg gggtaatcat ccctgatgtc agcaatatgt
    16621 tctttgcttc tttagcacgt ggtatcgatg atgtggcaac aatgtacaaa tacaatatta
    16681 tcttagcaaa ttcagatggt aacgaccaaa aagaagtcaa tgtattgaat aacttactgg
    16741 ctaagcaagt ggacggcgtg atcttcatgg gtcaccatat cacagacgaa atccgtggag
    16801 aattttcacg ttcaaaaact cctgttgtat tagctggctc tattgatcca gatgaacaag
    16861 taggtagcgt aaacattgat tatgtatcag caacaaaaga tgcagtaaac aaattggcaa
    16921 aaagcggcaa taaaaaaatc gcttttgtca gtggtgcgtt gatcgatcct atcaatggac
    16981 aaaatcgtct gaaaggctat aaagaagcat taagcgaaaa taacctacca tatagcgaag
    17041 gattgatctt tgaagcacaa tacaacttca aagatggtct atctctagtc gatcggatcc
    17101 ataatagcgg tgcaacagct gtctatgttt cagatgatga attagccatc ggtattttgg
    17161 atggtttatt agaccgtggc gtgaaagtac cggaagaatt cgaaatcatc acaagtaaca
    17221 attcactact gacagaagta gctcgtcctc gtttaacaag tatcacacaa ccattatacg
    17281 atttaggggc ggtatctatg cgcctattga cgaaaatgat gaataaagaa gaagtcgaag
    17341 aaaaaacgat tatcttgcct tatggaatcg aagaaaaagg atctacaaaa taataaaagg
    17401 acaaccattc agaggttgtg agcctgccgt ttaactctgc gttacaagga gcaatttcaa
    17461 aaaacagcat ttcatgtttg atgaatattg tgactcgtga ttcggagttg gcaggagaac
    17521 accgtttatt cgggaaaagg ggttatgaca aaattttgtt taatcctttt tttttgtctt
    17581 aaaaaagaga gaaagtggtt gaaattacat ttatcgtata taaaacaatc cgtgtataga
    17641 tgctgcatta tgttcaccac catcaaaaaa ataagggact gtgacaatac tattgtcaca
    17701 gtcccttatt actgtgtatt ctattaaatc aatcatcttt ctcatctgat tctttggttg
    17761 cttgattcgc tcttgcataa gctgaagcgg tgttccagta atctgtatag ttttcatcgg
    17821 taccgccaac gccaaattta aaggcgcttt tcttcggtcc atttttcgcc catagcgatt
    17881 caactgtttt tcctggtgtt tggatggatt ttttattgac agtaactttt ccaggtgttt
    17941 gaccagtaaa tccagatact tttttcttca tcacatcaga tgataattcg aatttctcat
    18001 cagtgccaaa aatgtcaggt tttgcttggt aagcttgatt gatcagattg gcaagataat
    18061 tagccgaacg tatccttgct ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc
    18121 tactgatggt aatagacgga gtagagacaa ttagccatga atctcggtat tcattggtag
    18181 aaccagtttt tccgacccaa tctgcttttc ctagatttcc gtttaagctt gagatggcat
    18241 ctttgaatgg tgtagtgatt tttgcattga tcacactgcg catcatgtca ttcatgatcg
    18301 atgccgttgc ttccgaatag atccggacag agttgctttc gtgtttataa attacatttc
    18361 ctgcgttatc tgtgatggaa tcgatgatat agccttcttc atagacgccg ccgtttgcta
    18421 aagcctgaaa tccattggtc tgttgaagcg tcgtgacatc tactgtacct aaaggagcag
    18481 attcaacgcc ccaattgtca tttgccgggt agttcatctt tgccaagtag tgctcgtaag
    18541 catattgttt cgatccaccg gaattcaaca catcttgata taagtgataa gccgggatat
    18601 tgttggacca ttctagtgac tcacggacgg tttggaaggt gttcgatcct tcgttagtag
    18661 cattgacgat tttctcgcca gcattttctc cttcttgcca agttgttgca taatctgata
    18721 cacgggattc tgatccgata agccccatgt cgattgcagg tccataaaca agaactggtt
    18781 tgattgatga gccagcttga cgttctgtat cgaaggcatg gttattttga ttctgactgt
    18841 aattccgtcc gccaacgaat ccgtagatgc ggccagttct gttatccatc aatacatttc
    18901 cggtttctac ttgactgcca gcaccatcat ctaacatgta tccataattt tctacgccat
    18961 tttgcaatgc agcatagaca tctttatcga ttgtcgaatg gattgtatat cctttatttt
    19021 ggatcgtttg ctgtgccttt tgataataag cgtcactgac cgatgaatca gacatatcag
    19081 cgttgtcttt ttcagccaat tgttgtgcaa tgattcttgt tgcttcattc atcacagtat
    19141 aatacaagaa ttctctgtca gtctgttctg cgatttgttg tggtagaaag tcttttgtaa
    19201 ggtcataggc ttttgcttct tcgtattgtt ctttcgtaat ttggccttca cgatacatac
    19261 tgaataaaac aaaatctttt ctggcaaggc cggcagaaag atcttctttt agcgcacctg
    19321 tgttcgtgta tggactatac gtgatcgggc tttggggtaa tccagcgata taagcagctt
    19381 gcggcagcgt cacatctttt gcatctacac caaagattcc tcttgcagct tcctggactc
    19441 cagcaatgtt ttgtccctta ttattacgac caaatggtga gacattcaaa tacgtagcaa
    19501 tgatctcatc ttttgaaaag tatttttcta cttgtgcaga aagaatgatc tcgtttgctt
    19561 tacgtttgaa tgttgtttca tctgtaagga tctgctgttt gactaattgc tgtgtcaaag
    19621 ttgatccacc agaagagcca ataccagtag cttcagatac taatgctcgt aaaacagctt
    19681 taggaacgta accttggtgt ttatcgaagt attcatcctc tgtgctgata attgcagttt
    19741 tcaataaagg tgaaatttta tcagatgaaa tagtcgtgcg catcagatct gtctggattt
    19801 ttgatatctc tgtattatct gcataaacaa tctttgacgt ttctgtaatg tttcctagtt
    19861 ctgtctgcag tgctttttta gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc
    19921 cagcgcctat tccaatacct agagctgcga acatcaaaag aagaacagta ataaaaacag
    19981 tcaatgattg aaaaactcta aaaataatct taggaacaaa ccatttttct ttttttcttc
    20041 tattctgttt ttttcgagta tttttgcgtg gcaaaagggt cacctcaact aatggtttta
    20101 tttcgctaat tatagcaaat aatagaacaa aaaaacagac aaggctgttt ttggcaggaa
    20161 tttctttttt aaaagagtta aaacgtgaga aaatgatctt ttttataaaa gagttcttaa
    20221 gcttttcttt agagaaaatt tcagttacta tggagaacag ggcaaagaaa aatcaatcat
    20281 tttctttgta tatcatctta ccaatatata ggaattgtta gtaacaattg cctttcaagg
    20341 aggaatccat ccatgttatt aggctcacac gtcagcatga gcggaaagaa aatgctgtta
    20401 ggagcagcag aagaagcagc cagttatgga tcttcgactt ttatgatcta tacaggcgcc
    20461 cctcaaaaca ctagaagaaa agccatcgaa gagatgaata ttgatgccgg aaaagcgtac
    20521 atggcagaaa acggtctaac aaatatcgtc gttcatgccc cttatatcat taatttgggg
    20581 aatacgatca aaccagaaat gtttcctttt gccgtccaat ttctgagaga agagattgtt
    20641 cgtgcggaag ctttaggcgc tactcaaatt acacttcacc caggtgctca tgtaggggca
    20701 ggaacagaag ccggaattgc tagaattatt gaaggcttga atgaagtttt gacaaaagaa
    20761 cagacggcac aaatcgcttt ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca
    20821 tttgaggaat tagcgcaaat catcgatgga gtaaaattga atgaaaaatt atccgttact
    20881 tttgacacct gtcatacgaa tgatgccggt tataatatta aagaagactt ttcaggtgta
    20941 atggaagagt ttgataagat catcgggtta gatcggctta aagtactcca tatcaatgat
    21001 tctaaaaatc cgcaaggttc gcataaagac cggcacgcca atattggttt tggaacaatc
    21061 ggattcgaca cgctgaataa gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc
    21121 ttggaaactc cctatgtcgg caaagataaa aagaaagctt acgcaccata tggaaaagaa
    21181 atcgccatgt tcaaagcaca aatattcgat ccagaagtgt ttcctgagtt attagcagaa
    21241 gaagtgatac tctaagattt tctatctgac agcagcaaaa aaggaccagg cacattgcct
    21301 tgtccttttt tgtttaagaa acaaaggagt gctttttgca gtctttttca tcatgcatct
    21361 tctcccccct ctttttaaat ttagacaaac agggtatact ggtagaaaag taaaaaaacg
    21421 aaaagtttca caagcaagtg gcaaaacgtg attggaagga cgaaaagatg ctgaacactt
    21481 taaaaccctt gatcaaccaa ctaaaaaaga aacatacata ctttattctt ggcatagccc
    21541 tattatttac aattattcgt ttgtatttat atagtaaagc aatctatgga acagacatgt
    21601 cggcaaccta tgatgaccag ctgctgattc gctatgctca aaatattttg gacggtaaat
    21661 ggctagggga gtatacgact acaacacttt ccaaaggaat ctcctacagc ttatttttag
    21721 tacttgcaca taaactgcat ctttcttatg cagttttgtt cggcatgttc agtatttttg
    21781 ccagtatatt ggttgcactc gcgtttaaac cgatcgtgaa gaacaagtat cttcttttaa
    21841 gtttctattt ttattttttg ttttctccga ttagttttac acatgaatat tccactcgaa
    21901 cgtatcgtaa tgccctcgtg attccggctg ttgagatcat tatcgcttgt ttactagcta
    21961 tttattaccg taaatatagt tccagcaaaa aaatgctgcc gtggttttta gggctagctg
    22021 tttcttttcc tttcttttgg ttcattcgcg aagattccat ctggcttctt ccttttactg
    22081 tagtcgcgtt aggcatcagt ttcgtccaga tcttgctagg acaagacttt tctttcaaag
    22141 aagcaaagaa gttaaaagaa cgaccatttc tcctttcgaa acaagaatgg ctgaaacttt
    22201 cattgttttt actccctcta gtcggtttct ccatcgtcta tcagtggatc gaatataaaa
    22261 acgagacaac atatagtatt gccgccatca atgaccgttc gagtggcgaa tttggacgtt
    22321 tgatgaaaca gctgatccga atagatgacg aaacgaactt aaataaagaa aacagtgaga
    22381 tatgggtctc gcatgctgca ttaaataaag cattagacgc atctcctaca tttcatgctg
    22441 tttctactcg tattgacgaa ttgtatacga ctcatgcgtg gacccaaggt ggaaaagtaa
    22501 aagaattacc aggagatatc attttttggg cattgcgaga tgtggtaagc gaatctggtt
    22561 attaccaaaa caatgcaaaa gaaaccaatg atttttggaa aaaagtcaat caagaattag
    22621 aagaagccta tcaaaaggga actatcaaaa agaaaaaaga attctatctg atgggatcag
    22681 gggatggaaa gacgatgaat gatctgccaa tcgtatttgc ttttctgaaa tcaggatatg
    22741 cctatgcatg gcgatacaaa gatttccatc aaggggggaa ttatagtttc ggtacgcaag
    22801 aacaagtcga acaggcccaa gaattattga atcttccact aatggataat tggatcgata
    22861 gcagtgatcc gagaacacct gatttccagt tgacaaaaac agcaaaagtc gcgaatgtca
    22921 tgataaaaat ttaccaagtt ctggccattc ccgtgctgat tgcttcaata gcagggatat
    22981 tacttatgat actaggaacc gcatttgcaa aagctgatcg tgcctattat agttcctctt
    23041 tgattgtagt caccggatta atactgacac aattggtttt tcttttcggc gtctcgtggt
    23101 tttgttctta tgcgccagaa aaaaaggatt attttttaag tgtctacact ggcgcaggcg
    23161 tgccattaac gcaattcatc gtagtaacaa gcatgctagt tttcagtaaa ttgaatctga
    23221 aaaaagcttt atcttccaaa taaaaacata ctaagattag aggagaacat cattgacgaa
    23281 tgatgttctc ctctattttc tatttttaga ttatagtgaa gagtgaaaga aagttcgaac
    23341 aaagccttga ggactatgag tccctatcaa aaaccgaact tctttactgt cattttagaa
    23401 tcaggtttac gtatgttggg gccatgagcc cgtgtatagg aaattggaat cgaaatgaag
    23461 cagtaaagtt ctttcaaaac ttaaaaagga ggaaaacaaa tgccttacat tcttgcatta
    23521 gatgtttcaa tgggacacag ctacagtgtc ctatacaaag acgacacctg cttatttgaa
    23581 gacgagatcg aacacactca aagaggattt catgcattac tcgaagagat ccagcacttg
    23641 cctgaacgtc cattgattgt ttttgaagca acggggattt attccagacc agtcgaaaag
    23701 ttctttcaag atcatcactt ttcttactgt ttgttaaacc cgttagaagc gaaaaaacag
    23761 atggaagaaa acaccttatg cagttggaaa ac
//
LOCUS       DAYYAR010000032        23653 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000032 DAYYAR010000000
VERSION     DAYYAR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.032"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..601)
                     /locus_tag="ACHAT0_001096"
     CDS             complement(26..601)
                     /locus_tag="ACHAT0_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8451054.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="ACHAT0_001097"
     CDS             complement(606..1580)
                     /locus_tag="ACHAT0_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM8451055.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="ACHAT0_001098"
     CDS             1863..2831
                     /locus_tag="ACHAT0_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8451056.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="ACHAT0_001099"
     CDS             2928..3221
                     /locus_tag="ACHAT0_001099"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8451057.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="ACHAT0_001100"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="ACHAT0_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8451058.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="ACHAT0_001101"
     CDS             3678..4433
                     /locus_tag="ACHAT0_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8451059.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="ACHAT0_001102"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="ACHAT0_001102"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8451060.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="ACHAT0_001103"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="ACHAT0_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8451061.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="ACHAT0_001104"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="ACHAT0_001104"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8451062.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="ACHAT0_001105"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="ACHAT0_001105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8451063.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="ACHAT0_001106"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="ACHAT0_001106"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8451064.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="ACHAT0_001107"
     CDS             9170..9712
                     /locus_tag="ACHAT0_001107"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8451065.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /gene="atpA"
                     /locus_tag="ACHAT0_001108"
     CDS             9735..11291
                     /gene="atpA"
                     /locus_tag="ACHAT0_001108"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8451066.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="ACHAT0_001109"
     CDS             11305..12207
                     /locus_tag="ACHAT0_001109"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8451067.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="ACHAT0_001110"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="ACHAT0_001110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8451068.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="ACHAT0_001111"
     CDS             13650..14072
                     /locus_tag="ACHAT0_001111"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8451069.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="ACHAT0_001112"
     CDS             14541..14771
                     /locus_tag="ACHAT0_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8451070.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="ACHAT0_001113"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="ACHAT0_001113"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8451071.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="ACHAT0_001114"
     CDS             16182..16358
                     /locus_tag="ACHAT0_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8451072.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="ACHAT0_001115"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="ACHAT0_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8451073.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="ACHAT0_001116"
     CDS             16853..17320
                     /locus_tag="ACHAT0_001116"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8451074.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="ACHAT0_001117"
     CDS             17405..18289
                     /locus_tag="ACHAT0_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8451075.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="ACHAT0_001118"
     CDS             18403..18639
                     /locus_tag="ACHAT0_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8451076.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="ACHAT0_001119"
     tRNA            18727..18807
                     /locus_tag="ACHAT0_001119"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="ACHAT0_001120"
     tRNA            18814..18885
                     /locus_tag="ACHAT0_001120"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="ACHAT0_001121"
     CDS             19011..19181
                     /locus_tag="ACHAT0_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451077.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="ACHAT0_001122"
     CDS             19401..20108
                     /locus_tag="ACHAT0_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8451078.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="ACHAT0_001123"
     CDS             complement(20167..20811)
                     /locus_tag="ACHAT0_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8451079.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="ACHAT0_001124"
     CDS             20938..21225
                     /locus_tag="ACHAT0_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451080.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="ACHAT0_001125"
     CDS             21333..21653
                     /locus_tag="ACHAT0_001125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8451081.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21734..23047
                     /locus_tag="ACHAT0_001126"
                     /pseudo
     CDS             21734..23047
                     /locus_tag="ACHAT0_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23044..23463
                     /locus_tag="ACHAT0_001127"
     CDS             23044..23463
                     /locus_tag="ACHAT0_001127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451082.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKRLPGIYLLSNTFI"
BASE COUNT         7820 a         4125 c         5092 g         6616 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa aagattaccc ggcatatatt tattgtccaa tacctttatc
    23461 tgaaaaaagg tggctagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaaa
    23521 aattcctttt cagatgctgc tcaaaagaga gcaacatctg cttgctcaag caaaaaacac
    23581 gcgaaaggaa agattggaga tgcctcagcc ttttgagctt gccgtacctc tttctaagat
    23641 acaacataaa aaa
//
LOCUS       DAYYAR010000033        23625 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000033 DAYYAR010000000
VERSION     DAYYAR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.033"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..665
                     /locus_tag="ACHAT0_001128"
     CDS             33..665
                     /locus_tag="ACHAT0_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451083.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(799..924)
                     /locus_tag="ACHAT0_001129"
     CDS             complement(799..924)
                     /locus_tag="ACHAT0_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8451084.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1058..1951)
                     /locus_tag="ACHAT0_001130"
     CDS             complement(1058..1951)
                     /locus_tag="ACHAT0_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8451085.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2133..3059)
                     /locus_tag="ACHAT0_001131"
     CDS             complement(2133..3059)
                     /locus_tag="ACHAT0_001131"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8451086.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3144..3905)
                     /gene="pflA"
                     /locus_tag="ACHAT0_001132"
     CDS             complement(3144..3905)
                     /gene="pflA"
                     /locus_tag="ACHAT0_001132"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8451087.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4149..6380)
                     /gene="pflB"
                     /locus_tag="ACHAT0_001133"
     CDS             complement(4149..6380)
                     /gene="pflB"
                     /locus_tag="ACHAT0_001133"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8451088.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6657..9107)
                     /gene="parC"
                     /locus_tag="ACHAT0_001134"
     CDS             complement(6657..9107)
                     /gene="parC"
                     /locus_tag="ACHAT0_001134"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8451089.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9119..11164)
                     /gene="parE"
                     /locus_tag="ACHAT0_001135"
     CDS             complement(9119..11164)
                     /gene="parE"
                     /locus_tag="ACHAT0_001135"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8451090.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11330..11764)
                     /locus_tag="ACHAT0_001136"
     CDS             complement(11330..11764)
                     /locus_tag="ACHAT0_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8451091.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11891..12529
                     /gene="plsY"
                     /locus_tag="ACHAT0_001137"
     CDS             11891..12529
                     /gene="plsY"
                     /locus_tag="ACHAT0_001137"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8451092.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12661..13536)
                     /locus_tag="ACHAT0_001138"
     CDS             complement(12661..13536)
                     /locus_tag="ACHAT0_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8451093.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13618..14409)
                     /gene="codY"
                     /locus_tag="ACHAT0_001139"
     CDS             complement(13618..14409)
                     /gene="codY"
                     /locus_tag="ACHAT0_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8451094.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14427..15827)
                     /gene="hslU"
                     /locus_tag="ACHAT0_001140"
     CDS             complement(14427..15827)
                     /gene="hslU"
                     /locus_tag="ACHAT0_001140"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8451095.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15841..16389)
                     /gene="hslV"
                     /locus_tag="ACHAT0_001141"
     CDS             complement(15841..16389)
                     /gene="hslV"
                     /locus_tag="ACHAT0_001141"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8451096.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16426..17331)
                     /gene="xerC"
                     /locus_tag="ACHAT0_001142"
     CDS             complement(16426..17331)
                     /gene="xerC"
                     /locus_tag="ACHAT0_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8451097.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17529..18905)
                     /gene="trmFO"
                     /locus_tag="ACHAT0_001143"
     CDS             complement(17529..18905)
                     /gene="trmFO"
                     /locus_tag="ACHAT0_001143"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8451098.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18874..20952)
                     /gene="topA"
                     /locus_tag="ACHAT0_001144"
     CDS             complement(18874..20952)
                     /gene="topA"
                     /locus_tag="ACHAT0_001144"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8451099.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21074..21931)
                     /gene="dprA"
                     /locus_tag="ACHAT0_001145"
     CDS             complement(21074..21931)
                     /gene="dprA"
                     /locus_tag="ACHAT0_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8451100.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21987..22754)
                     /locus_tag="ACHAT0_001146"
     CDS             complement(21987..22754)
                     /locus_tag="ACHAT0_001146"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8451101.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22747..23607)
                     /gene="ylqF"
                     /locus_tag="ACHAT0_001147"
     CDS             complement(22747..23607)
                     /gene="ylqF"
                     /locus_tag="ACHAT0_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8451102.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6147 a         5100 c         4066 g         8312 t
ORIGIN      
        1 ttatatactt atcaatttaa tttggagata ttatggaaat tcaacgtaga accactaatt
       61 ttttacattt aggctctttt tttgcaactt tgacgatctt attttttcta tgttattcat
      121 accttaagac ctcacatgcg ccttctagtg cattaggctg gttgcttttg gatcattctg
      181 atatcacaga agccgaagaa aatccgtttt ttttgatttt gtcttctctt tgcggcttac
      241 tgttttctgt ttatttcggt gcctattttt ttcatcgaca tcctggtacg ttaaacgaag
      301 cttcaagcgt attgattgca tttaaaacgt tttctttttt agcaattctt ttttattcgt
      361 ttaatcacaa tacgcttttc ttctttatca tgaatggttt agtcgtgatt ttatccattt
      421 ttacttattt tattttttca ctcattttta gccaattgaa atgtccgctc ttctttcgat
      481 tagttcctgt cacctgtttt atttacagct tgttttcctt ttttgtcctt gctttaatgc
      541 cactgaccaa cccttcgacg atccaagtcg caggaaatct gttggatatg ctcttcatcg
      601 tcagtctagg tgtatttatg tggcaaagaa aaaacaaaca aaataaaaag cattttgaag
      661 cctgactatt acgacttcat aaatgctttt ttagttttaa acacttcaat ctaaaagaat
      721 aacccaagat tggataaatt attaatgact cgccattcgt ttcttagtga ttctggatta
      781 tcaaatggac ttcctaatct aatcagctag ttcttcccat aaaatgccat acttgactga
      841 atcataatat tgattattta aaaatctaac ttttcttatc actacttcct ttgtcattcc
      901 acatttctca ccattttttt gcattccttt gtttccagac catgtagtga atctaatagg
      961 tggtagatag gcaaaatact caaataattc ttttaaccat aaaggaagag aaaggctcct
     1021 cctgcaagag tcagtataat ttttgcttac tgctctctta ggttttactc actaaaactg
     1081 ttccatattc tttgatataa gaaaatgcat gcggtataaa tttcttgctc agctcagaaa
     1141 agatttgtcg tttaagaaag gccatttcct ccattgtcaa tgaaggatcc aatgttggta
     1201 attcaatctc aaaattgttt ttttcgacaa taggtaattc taatgtgaat gattcttctt
     1261 cctttatagt attcgtcaca agtagagact gatggaaaat actaggccgg cgatttttat
     1321 atttattctt acgtatcctg tcaacgataa cttctatttg atcaaaaaag tacgattcat
     1381 ttacgtctcc aataagttcg gctaattctt tgaatgcctt cgttacagga agctgattct
     1441 tctgtacaaa aaagtaatta gccaaagtaa gcgggaagtg ttctatagag gaaagtgtga
     1501 taaaacggta cttctcattt cctgcagttg tcctcagcaa ttctgtctct cggctgtttc
     1561 ttactccata aaaatacgct tctttccgtt catcttcgaa acaatacgcc caaataactt
     1621 ctcccatcgc taatttttgt tcatcaacag agagtctctt caattgttca gctatggttt
     1681 cagcagccga agtggcttgt tgcaagtaat ctttaggatc aaatattgga aagttcaaat
     1741 gatatcttcg ttcttccctc ttgattaatc ctgcatcgat caataaatca agaaaatggg
     1801 aaaatttatt ttgcgaaaat tcttttttca attctcttag aatcgtttct tgttctcgat
     1861 gttcatatag ataatcggtc aatgaagaaa aaagcggatc atcggtcagt ttttgataaa
     1921 agttttcctt attttttgaa taatagttca tatatgttct cttttctatc cgttttattt
     1981 tacttaaaag gcttttacgt aaaaaggagg aagaacaaga gtcataattg actgttattc
     2041 tcctccattt cttcggcctt tagtatgttc tatgtttctt tttatatcaa tcgcttatcc
     2101 attcattttg aatgcttcat actaccattt gattagctaa atacagccgt caattgaggt
     2161 acgacttgtt ttttacgtga aacgacacct ttaagcaaag cacggttgtt ggtcaaagtt
     2221 gtttggaaag cttcttctac tttttctttt cctttaccag caacaagtaa ttctgaatca
     2281 ctgtctaaga tgtttgtgac aactaatacg aacagatcgt agccatttgc tttcatttca
     2341 tcttccattg ctgcttctag ctctgcttgt cgatccatca cttcattaag gtccactgta
     2401 ttgacttgag cgatacgaac gttggaactg cccattggga aactttttgc atccaaatca
     2461 agaagaaccg cagctgattt atcacttaga tttgttcctg ctttcaacat ttcaagtcca
     2521 taagtgttta gatcgacttc agcgattgct gcaagttctt ttgcagctgc ttcatcttca
     2581 ggcgtacatg ttggtgattt gaacaacaat gtatctgaaa tgatagctga gagcatgatt
     2641 cctgcgattt ctttaggaat tgttacttgg ttttctttat atagttttaa aataatcgta
     2701 cttgtacatc caactggttc tgcacgatag tacaatggat tagctgtttc aaagtttgca
     2761 atacgatggt ggtctactac agctaaaata tttacttcgc tgatatctga aatactttgt
     2821 tggaattcat tatgatccac aagcatgaca tctgcatttt ctgctttagc tgactctact
     2881 actcgtggtg cagatagctg aaagtggtct aatgcgtatt gtgtttcttc attaggtgta
     2941 cctagagcaa ctgcttctgt ttctttacct aattcatttt gcaagtgtga aaacgcaatg
     3001 gctgccccga ttgcatctgt atctggattt tggtgaccaa aaactaaaat ttttgacatg
     3061 tcattcgctc ctttgtgatt tctcctattt aataatagca aaaaaacgga aagaagcaat
     3121 acttatcttc tttccgatgc gacctatcgc gttaaatatt ttgtatactc ttctgtatga
     3181 agcaatcgtt tagcattttc gacacgttct tttgtaggtg gttcgatccc ttctaatgga
     3241 tatttcaaac caagtgtttc ccacttgtat tttcccatcg tatgataagg aagaatctct
     3301 actctgtcta catttgacaa ggtttgaata aagttgtcta gtcggatcaa atattcatca
     3361 taatcacttc gctcaggaac tagtacatgg cgaatccaaa ccggtttgtt gatttttgac
     3421 aaatattgag ccatttctaa aatattttca ttgtcccaat gcgttagttt tttatgctct
     3481 tcattatcga tgtgtttgat gtcaaataac agtaaatctg tatacttcat caattcttga
     3541 aaacgtgaaa agaaaggttc ttcatacgta aaaggttttc cgcatgtatc caatgtagta
     3601 tgcatcttcc gctcttttgc ttttttaaat agttcgatca aaaaatcgat ctgcaataga
     3661 ggttctccac cactaacagt aataccacct ttatctcccc agtaaggtct gaagcgttct
     3721 gctttatcta aaagttcatc tgctgtgtat tctttccctc cgccaatatt ccaagtatct
     3781 ggattgtgac aaaattggca acgcatgcgg catccttgca taaacacgac aaagcgcagt
     3841 ccaggtccgt ctactgaacc gaaagtttcg atagaatgaa catagccgat tgttttttct
     3901 tccatttaag ttcacatctt ttctttgatt tcattttaag tacaagttag ctgtcgaagc
     3961 gaagctttct gaacaaaatt tttcgcaatt gccgcttcgt tatgtgtaaa atttacaggg
     4021 acggaacata caagtcctgc ccctgtaaat cgtttctttc aaataagcat actgattatt
     4081 cgattttgat gcgatggcta ttcaacgtcc tttcgacgct aagtcgaaaa acttttttca
     4141 cgaacttttt acattctgtc gtgtgaagtt ctcatcaaga catctaattg ttgttctctt
     4201 gtcaagtcac ggaatttaac tgcataacca gatacccgga tcgtcaagtt cggatatttc
     4261 attggatttt ccattgcatc acgcaataaa tcattactga atacattgac attcaaatga
     4321 taagctcctt ttgagaagta tccatctaag acattacgca agttttcgat tcttgtatct
     4381 tgttcttttc caagacctga aggattgatt gtttgggtat ttgaaattcc atccaatgca
     4441 cagccgtaat tcattttagc tgttgagttc aggctagcta ataacccact ttgttccgca
     4501 ttgtaacttg gatttgctcc tggcgcaagt ggttttccag cttcacggcc atcaggagta
     4561 tttcctgtat ttttaccgta aacaacattt gaagtaatcg tcaataatga agttgttggt
     4621 tttgagttgc ggtaagtttt atgacgtttg atttgtccca tgaagtattc caagatccat
     4681 gaaactaatt catctgcacg gtcatcatca tttccgtatt tagggaagtc gccatccact
     4741 tcaaaatcaa ctgctattcc tttttcatta cggattgctt ttacttttgc atatttgatc
     4801 gctgacaagc tgtctgccgc atgagagatc ccagcaatac ctgtagcaaa tgtgcgttgc
     4861 aagtcagaat ccatcaaagc cagctgagct gcttcataag aatatttatc atgcatataa
     4921 tgaatgatgt tcaacgtatt gacatatagt tctgctaacc aatccatcat attcatgaag
     4981 ttatgtttca cttcttcaaa gtctagtatt tctgatgtga ttggacgata ttctggtcca
     5041 acttgggcgc cagtcacttc atcgactccg ccattgatgg catataatag tgttttagcc
     5101 aagttagcac gagcgccaaa gaattgcatg tcttttccga caactgttcc tgatacacag
     5161 caagcaatcg cacaatcatc agaaccccat tgttcacgaa gctgattatc attttcgaat
     5221 tgaatcgaag aactttcgat agcgattctg ctagcatatg ttctgaatcc ttctggaagg
     5281 tgttcagaat acaaaacagt caagtttggt tctggtgaag gtcccatatt tgttaatgta
     5341 tgaagcagac ggaaatcatt ctttgtaact aatgaacgac catctaatcc catacctgca
     5401 atggataaag tagcccagat agggaatcct gagaataatt ggttatattc aggtgtacgc
     5461 gcgaatttga ccatacgtaa tttcattact aagtggtcga tcaattcttg tgcttctttt
     5521 tctgtgatta ctccattatc caaatcacgt tggatataga tatcaaggaa tgctgagata
     5581 cgtccgattg acatcgcagc accattttgt gatttgattg ctgctaaata tccgaagtat
     5641 agccattgga cagcttcttt tgcatttgca gctggtttag aaatatcata gccatatttc
     5701 gcagccattt ctttcatttg cgctaatgca cgatattgtt cagtgatttc ttcacgtaaa
     5761 cgaatcactt cttcactcat tgtatgataa ccagtcatat catgatcttt tttcttttgt
     5821 tccatcaaat agtcgattcc atacaatgcc acacgacgat agtcaccgat gatacgtccg
     5881 cgaccgtaag cgtctggaag ccctgtaatg attttgtttt tacgagcagc acgcatttca
     5941 gcagtataag cgtcaaatac accttgatta tgtgttttgc gccagtcagt gaaaatatgt
     6001 gtcaattcat cgtttggtgt atagccattg ctttttagcg cattgttagc catattgatt
     6061 ccgccgaacg gcatgaatgc ttgtttcaaa ggtttgtctg tctgtagacc gacgattttt
     6121 tccaaatctt tatccaaata gcctgcatcg tgtgaagtaa tagttgatac gatttctgtg
     6181 tccatatcat agacaccgtt acgctcgaat tggatatcat ttaacttttg caattcatcc
     6241 cataattttt ctgttgcttc tgttgggcct tccaaaaagc tgtcatcacc gcgatattca
     6301 gtgtagttat tttgaataaa gtctctagtg tctacgcctt ctttccattt ggtaccttta
     6361 aatccattcc attgctccat tgtatggacc tcctaaattt ctgttttatg taacagtttt
     6421 tacttgatta gtataacaca gcaccctatc tttgcaaggg ttttcttgtt ttttatttaa
     6481 caatttttat gttttattta catatattta aatcaacgtt gacataccaa catttaagca
     6541 ttgtgaacac tttcatcttt ttttgcaaac gtataaggat ttattaaaaa tgaatgaaaa
     6601 agagacgcca cctgtattaa tacagatggc gtctcttcat taaaatacta caacagttac
     6661 gactcgatga aaatctcagg agcatcgata acttcgatga cttctccacc ttttttctca
     6721 tccatcgcaa atgatccatt cgatgttcga tcacctatag ggaaatcagt tgggtcaatc
     6781 gttaactgct gccctttttg attcatcaag gttatttctt ttgatcttcc ttccgacata
     6841 tagataatac ggtgcggatt ttttttcagc tcacgcatga ccgtcactcc ccgcttcgca
     6901 cgtgaagttt ggctgatttc ttgagcaagc atgcgtttaa cagctcctcg ctgagtaacg
     6961 attacgatcg gcgtatcacc ttctgctaac acaagcagcc cgttgataat cctatctcca
     7021 tctttcaaat tcatagactt cactccagct gcctttgctc caacaacggg aacatctgtt
     7081 aaaggatagc gcaaacccat tccttgatgc gttactaaga aaacatctaa gcctggctga
     7141 tcttctttca gatacacagc aatcagttca tcttcttccg cctttagctt catacagctg
     7201 agcggacggc tcttataggt gcgccatggt tcgaactctg tcattcgtgt ttgcttgatc
     7261 aatccattct tgctgatgaa tacaaatgtt ttctctgctt gaattttttg atatgggaaa
     7321 acagctaaaa tcgattcgtc tacagacaga ttggagattg tttgagaaat gtgctcacca
     7381 gcatctttcc atttcaaatc gggtagttca tggacagggc ggtagatcac atttgcttta
     7441 tttgtgatca gaagaacatg gtccaatgta ctcaattcac ctgagtatag caagtagtcg
     7501 ccttctcgca tcccgatttc ttctggtttg gaagcactat aagaacgaag gcttgttcgc
     7561 ttcacatagc cttctcgcgt cacactgaca attacgtctt cttgtgccac taagacttgc
     7621 gtatcaattt tgatttcttc gatttcgtct tcgattgtcg ttagacgagc ggaagaataa
     7681 ttttttttca cttctcgcaa ttcttttttc ataacagaga aaagctcttt gtcatttgaa
     7741 aggattttat tcaactctgt gatttgcgca atcaagcttt cggattcctt ttgaagctct
     7801 gtgatatccg tgttcgtcaa acggtataat tgcaatgtga caattgcttc tgcttgttct
     7861 tctgtaaatt gaaagacgtc gactaaattc tttttggcgt cttttttgtc tttactttca
     7921 cgaatcgttg cgattacttc atccagaatc gataaagctt tcatcaatcc ttctacgata
     7981 tgctgtcttt tttgagcttt ggctaaatcg aattgactac gttttgttat gacttgcttt
     8041 cgatgctcga tataacttct taaaatgtct ttcagtccaa cttgatgcgg agtcatatgg
     8101 tcgatagcga ccatattgaa attgtaattg atctgtagct cagtgttttt gaacaaataa
     8161 ttcaaaattc cttgagcatt ggcgtctttt ttcaattcga cgacaatttg cagacctgtt
     8221 ctatcacttt catctcgtac ttcagctatt ccatcgattt tcttattcaa acggatctca
     8281 tccatttttt tcaccaaggt cgctttattt acttcatatg ggatttctgt gatcacgatt
     8341 tgctgtttat tacccttcat tgcctctatc tttgttttcg aacgaagaat aacttttcct
     8401 cgtcctgttt cataagcttt tttgatttca tcttttcctt gcaagatccc tcctgttgga
     8461 aagtcaggtc caggaatata ttccatcaat ttttcaaggc tcgcttgtgg atgatcgatc
     8521 atatagattg tgccatcgat cacttcagct aagttatgag tcgggatttc tgttgcatat
     8581 ccagcagata tacctgtcga tccattgacg agaaggtttg ggtatcgagc tggaaggact
     8641 gttggttctt tttctgtatc atcaaaattc cacacgagat cgactgtttc tttctcgata
     8701 tctgctagca tctctccact taacttggat aatctggctt cagtataacg catcgcagca
     8761 ggtggatcac cgtccatact cccgttattc ccgtgcattt cgatcagtac ttcccgcaac
     8821 ttccagtctt ggctcatgcg gaccatcgct tcataaatac tgatgtcacc atgaggatga
     8881 taattcccca tgatatttcc gacagatttt gctgatttac ggaatccttt gtcatacgta
     8941 ttgccatctt tgttcatggc aaataatatc cggcgttgca ctggcttcaa tccatcacgg
     9001 atatcaggca gtgcacgttc ttggataata tatttggaat aacgtccgaa gcggtcaccc
     9061 atcacttctt ctaaagttaa ttcttgtact tcttgtcgat tttccatcag gtgacctcct
     9121 attctacttc aaataactga ttgttttcat ttttatctgc tcgttcttca tctaataaat
     9181 cattagaaac tgaaggagag atttcagtat cctctttttt atctaagata ctgccatctt
     9241 cttcgagcgt aaattgcacg tgattttcga tccattttct tcgaggttct actttgtcac
     9301 ccatcagtgt ggttactcga cgttcagctt gcgcagcatc atcgatacgg acgcggatca
     9361 acgtacggga agtcggatcc atcgttgttt cccacaattg ttccgcattc atctctccaa
     9421 gtcctttata tcgctgcagc atatatcctt tgccgacttt tttaatcatt gctgccaatt
     9481 cgtcatctgt ccacgcatat tcaatgactt gcttctttcc ttgtcctttg gatactttat
     9541 ataaaggcgg taacgcgata tatactttcc cagcttcaat cagcggtttc atataacgat
     9601 aaaagaaagt aagcagtaat acttgaatat gggcgccgtc tgtatccgca tcggtcatga
     9661 tgattacttt gtcgtaatta cagtcttcga tagaaaactc aggaccgact cctgcaccaa
     9721 tcgtatagat catcgtattg atttcttcat ttttcaagat atcttgcatt ttagcttttt
     9781 cggtattgat gacttttcct cgtaaaggta gaattgcttg gaactttctg tctcttcctt
     9841 gtttagcaga tccgccagca gaatcccctt cgactaaata caattcattt ttcttagggt
     9901 ttcgggattg tgcgggtgtc aattttcctg aaagaagaga ttcgcctttt ttacgcttct
     9961 tcccatttcg gctttcttct ctggctttac gtgctgcttc tctcgcttct ctggctttga
    10021 ttgctttacg gaccaacatc tggctcattt cgctattttc ctgcaaataa aagcccattt
    10081 gttcactgat gacgttatct actacggtac gagcaactgg cgtaccaagt ttttcttttg
    10141 tttgcccttc aaattgcaga agattttcag gtacacggat agaaagtaca gcagctaacc
    10201 cttctctgaa atcactacct tccagattct tgtcccgttc tttcagcaaa ccaacttttc
    10261 tcgcatattc attataagat ttcgtcatcg ccgctttcat accagcttca tgggttcccc
    10321 catcttttgt ccgaacgtta ttgacaaaag ataaaacatt ttctgaataa ccgtcattgt
    10381 attgataagc gacctctact tcgatacctt ctttttctcc tgaaaaataa acaactggtg
    10441 tcaatgtatc tttttcttca ttcagataat caacaaattc tttgattcct tcttcgtaat
    10501 gaaaaatttc cttgactggt tcttctccac gaagatcaga aagagagatt ttaacgcctt
    10561 ttagaagaaa ggcagattct cttaaccgtt cggctaaaat ttcatatgaa aatttcgttg
    10621 tactgaatat tgtatcatct ggtaaaaaat ggacagtcgt cccgtttgct ttttttgttt
    10681 ttcctatctt tttcaaagta ccgtcaggtt taccgccgtt tttaaatttc tgctgatact
    10741 ctacgccatc tctaacgatc gttacaatta gccatttcga taaggcattt accacactgg
    10801 ctcctacacc gtgcagtcca ccagaagttt tgtaccctcc ttgaccaaac tttccgccag
    10861 catgcagcac agtaaaaatc acttcaactg ttggtattcc ggaagcatgc attcctacag
    10921 gcatcccacg tccgctatct gcaacagtta tactattgtc ttcatggatg gtcacatcaa
    10981 tctcactgcc ataacctgat aatgcttcgt caactgcatt atcgacgatt tcatagacta
    11041 aatgatgcaa gcctcggctg tcagtggatc caatgtacat ccctggtctt tttctaactg
    11101 cctctaatcc ttccaatact tggatggacg agtcattgta ttcgttgttt actttttttg
    11161 ccaaggcaaa cgctccttca ataatacttt gcggcaaaac cgcaccacga ttatcatact
    11221 agaaaaggtc gtgaaaaaaa agagggtaga cgagaaatca attttaatta tctcctaacc
    11281 gctttttcga cattcccctc tatcgatcat ctcttctttg tggtcatttt tatttttggt
    11341 tttctttcaa cttaccaagt tcaatcttaa tacaacgatc cattacgata tctgtacgtc
    11401 ctgcttttct tagcatctca gctgcttctt tgttttcaat ccctaattgt gcccagaaca
    11461 ctttagcatc agttttcaaa aaatcacctg ctatttcagg taaaaattca ctccgtctaa
    11521 atacatctac gatatcgatt ggatcatcga tttctgtcaa attcgcatag actttctcgc
    11581 ctagaatttc ttcaccagcc aataatggat ttactggtat gattttatat ccattgtctt
    11641 gcaatacctg tgcaaccatg taacttgttc gttctggttt attgctcaag cctacgacgg
    11701 caatattttt tgcttcttcc aaatagttga aaatcgtttc ttgtgttgga ttttttacag
    11761 acatttctgc caccccttgc ttttcttttt attccatcta ttatacttgt atctttcatt
    11821 ttttcaattg ttcctgttac ctattcatgc taaaataaag gatagttttc aacttgaaag
    11881 agggtaaaaa atgaaattga tcttattact tattttagca tatcttttag gttctattcc
    11941 ttccggtgtc tggattggca aattgttttt caaaaaagat atccgacaac acgggagcgg
    12001 taatactgga acgaccaata catttcgtgt attaggcaaa acagccggga ttgttgtcct
    12061 gcttatggat attttaaaag gtacattggc tacttgtttg ccgatgatct tccacctttc
    12121 tgggattaac cctctttggt ttggggtatg cgccattctt ggccatacat ttccgatttt
    12181 tgctgggttc aaaggaggaa aagctgttgc gacaagtgca ggaatgcttt taggatttaa
    12241 tcctgtattt tttgtttatt cttgtatcat ctttatcgtt agtttatttt gtacaagtat
    12301 ggtgagtttg agcagtatga tcagtgcagt attgatcact ttatctacga tcgtccttcc
    12361 ttatgtagct cctgtgattt tggctaagcc caattggtta ctgacgatca ttgccttttt
    12421 agtttcttcc tttatctttt atcggcataa agacaatatc aagcgtatca gaaaccatac
    12481 tgaaagtcga atcccgtttg gtttgaatag cagcaagaaa aaatcctgat actttttcat
    12541 tccaaagaga taaaacggga gtgtgacaaa tcgatgtcac actcccttat tacttacagg
    12601 atagcttttt tacacacctt ggtaaacggt gttccctgct aacttctcgg tcacaacctc
    12661 ttacttcact gtgatggaat attttgtctt gaagatttca tgggcaccta gttgattgat
    12721 ccctactttt tccatcaagt ttccactact gttcaccgtg tcagcgattc cgcaccacgg
    12781 ttcgatacaa acgaaaggcg cttcttttgg ataaggagac cagatcccta cataaggcat
    12841 atctttatag cttaaagtta cgctatggga agatttttca cttcgaatgg cgtacgcatt
    12901 tagacccctt gtttcaaaaa ccaaggcatc ttgctcaaaa agttcacgat tcaaagccag
    12961 attcgtattt gtctgaccta acgtcttttg atccatatcg atgaatgggc ctttcaatgg
    13021 taggcgtaat cttgatttca atggagaaaa tgccagatag aaatcttcaa atgacaatcc
    13081 ttcttcaaga ggtacattga aagcaggatg tccaccgata gaaaagaaca tttctttttc
    13141 accggtattt gccacttgat aatgaaccgt gatgccgtct cctcctaatt catagctgat
    13201 cactaattca aaatcaaatg gatacttttc taatgtctcc ggcgtactcc ttaagatgaa
    13261 actagccatt tcttgtccat gttcaatgac ttcaaaatcc atatcccgag caaatccgtg
    13321 ttgccccatt tgatatgttt tcccttgata aacatactga tcatctttta aacgtccaac
    13381 aaaagggaat aaaaccggtg catgtctgcc ccaatacgct ggatcacctt gccagatata
    13441 ctctaaacca tgttcttttg atttcagact gatcaattcc gcaccgtgtt cagcgatttc
    13501 ggcgatcagc tggtcatttt gaataataac agtcattgcc attcctccaa cttcgactta
    13561 ctttttaaaa tgggaaggct ggaacaaaca tttctatctg ccccagtccc cttacttcta
    13621 ataactgtgt tttgctaact cgtctttaaa acgggaattc aatactttta aataagttcc
    13681 cttcatgcct aacgaacgag attcaatgat tccagctgat tccaattttc gtaaagcatt
    13741 gacgatcaca gaacgagtga tgccgatact atcggcaata ttagaagcag tcagacgacc
    13801 ttcatctcca tccaatgctt caaagatagc ttgtacagcc ttcaattcgc tataagataa
    13861 tgtgttgatt gccatttgga cagccgttgc actccgtaca tctgcttcga tgcttcgaga
    13921 cttctgataa aggatctgca taccgacaac cgtagctcca tattctgcta agactagatc
    13981 gtcatctgca aacgattgtt ccatacgaga taagataatc gtacccaaac gttcgccagc
    14041 tccaaagatc ggaacgacag tcgtcagacc aaatggatat ttctctcttg actctaccgg
    14101 aaatgcagta atatcactag taattgggat attagcttca gttttcatca actgtgccac
    14161 tgtatcagta taagattcag gaaattgttt ctcaacaaac attgttttga ttcgttcatt
    14221 gttcacatcg tgacgttcat tataacctag taatactccg tcacttccta tgatataggt
    14281 gttgctgtca agaataccac ctagtgtgac agccatttta tcgtaaggca aatctgcttg
    14341 catgtcaaaa gtattctttt gctgcaaaag tgtgtttatc ttgcgggttt tcgttagcaa
    14401 gtctgtcatt gtcgtcctcc gattttttat aaaatatagc gacttaaatc ttcatttgcc
    14461 gcaattgagc caagtttgtc atccacatat gcttctgtga tcgttatttc tcccatttgc
    14521 atatctggtg cttcaaataa tagatcttct agtagttttt ccaaaatggt atgcagtctg
    14581 cgtgcgccga tattatctgt gtcacggttg acatcgaaag cgatttgagc gatccgttca
    14641 attgcttctt ttgtaaacgt aactgacaca ttttctgtac cgatcaacgc gacgtattgt
    14701 tttactaaag cattatttgg ttcggtcaga atcttcacaa aatcttcagc tgtcaaatca
    14761 tctaattcta cccgtatcgg gaagcgtcct tgaagttctg gaattagatc acttggttta
    14821 gacaaatgga aagctcctga agcaatgaat aggacgtgat ctgtttggat gatcccgtat
    14881 tttgtgttta cttgtgaacc ttcgacaatt ggcaatatgt ctctttggac gccttctctg
    14941 gatacttctc cagattgctg tgatttagaa gtgatcttat cgatttcatc gataaaaata
    15001 attccacttt cttccgctaa tttgattgcc tcactatgga tatcagcatc cttgactaat
    15061 tttgctgatt cttccttgat caataactct aaagcttctt tgactgttac cgtccgttcc
    15121 acttttttct tcggtgtcaa agcacctaga gtttcattta ggtcgatccc catttgctcc
    15181 aatccattgt tcatcattgg tgcaggtttc ttttggtctt caacttcaat ggttacttca
    15241 cgactatcca gtaaaccctt ttgtagttgt tccaagactg tacggcgatt gattcgaata
    15301 tcttctgtca cttcttcttg cggttcagcg gtattttgct gactagcatt aaaaagattc
    15361 atcatttgtt caaattgatt agtggattgt ttctgttctt ttttgatacc aggtaccaag
    15421 atttttacca aacgtttttc tgcatttttt ttagcacttg tgtatacacg gctgtactgc
    15481 tctttttcta cgatttggat cgcattttct actaaatccc gaaccattga ttctacatct
    15541 cttccgacat aaccgacttc agtaaatttt gttgcttcga ctttgacaaa tggtgcattg
    15601 acgatttttg ccaaacgtcg tgcgatttct gttttaccga cccctgtagg tccgatcatc
    15661 agcatatttt ttggtgtaat atcttgttgc attttttcat ctaactgcaa acggcgatag
    15721 cggttacgca aagcaacggc aacagatttt tttgctgcgt gctgtcctac gatatattca
    15781 tctaattcac ttacgatttg tcttggtgtt tgttttaatt catacatatc tactcacctc
    15841 ttacagttct tctacgataa tattatgatt agtaaagata cagatatctg cggcaatatt
    15901 taacgcattt tctgcaattt ctttcgcact catgtcgttt tgagcatagt ttttcattgc
    15961 tcgggctgca gccaacgcaa aattccctcc tgatccgata gctaagatac catcatctgg
    16021 tgtgataact tctcctgtac ctgaaacaag aagcatttct ttatcattca ttacgatcag
    16081 catcgcttca agtttttgca tagattgctg tgtccgccat tcttgagcta attcgacagc
    16141 tgcgcgttgt agattcccgt tatattcgtt tagtttgcct tcaaattttt cttctaaagt
    16201 aaacgcatct gcaacacttc cggcgaaacc tacaacaact tctccattgt agatacgtcg
    16261 tactttcttt gcagtgcctt tcatgacaac agattcaccc attgttactt ggccatctcc
    16321 agccatagca aatttcccgt ctttttcgac ggcacagata gtggttgaat gaaatggtga
    16381 ttcgaccatg tttgtttcct cctaaatttg ttcttttcct tatttttacg ctcttggatg
    16441 gaaagaacga tagttttttt gtaaactttc ttttgttaca tgggcataga tctgggtagt
    16501 cgataaattg gcatgtccta gcaattcttg gactgtccgc aaatctgccc cattatttaa
    16561 taaatgagta gcaaatgtat ggcgcagcat gtgaggatgg atatcactat ccaaactgct
    16621 ttttttgatc atttgattca agacatattc aattccagta ggcgtgattg gttttccatg
    16681 atgattcaca aaaacgatat catgctcttc gtgatatttt gtcatcaata cctggcggcc
    16741 ttccctgaaa tattcttgca gagcatcttg agcaaaagaa cctagaggag catatctctc
    16801 tttgttccct ttaccatgga tcaacatgac ctctgaatca aaatcaatgt ctgataccga
    16861 caaactgcta cactcactta aacggatgcc tgacccgtaa aggacttcca gtaaagcgcg
    16921 gttcctcaaa tccaaaggac tatctcctgc tacgctgtca aaaagtgctt gcatttcatt
    16981 ttcataaaaa aaacgtggca gtctcaaatt tttctttttg agatgaacgt aagaaaatgg
    17041 attttctttt atcacttctt ctttgagaag atactgataa aacgaacgta ggctggctat
    17101 cttgcgactg atagaatttc tgctatagct aggacgatga tcagctaatg cactcaaata
    17161 gacacgaacc gtaaaatatt ccgccttcag cagatcagcg tctccagatt cctccaaaaa
    17221 atgtttgaaa tggagaatgt cttcttcata tgcttctttc gttttttctg aatatcctct
    17281 ttcgacaatc aaataacgca aaaatttttg ttcccacgtg gcttctgtca ttttctcacc
    17341 ttccttttgt cccagagaat aatttagcac agcgtttata aaaagacaaa taaattgtca
    17401 gaatttaacc aatatttcta agtttgttca caattggcgc aattcctcac attttgtttg
    17461 agaaattctt aagaaaaacg caagaaaaag agcgagacta tccgtctcac cctcttcatg
    17521 gagtttcctt actcatttgg gattgcagtt ttttctaatt ctgtttttat tgcctcatta
    17581 gcttttaaag ctcgttgtgc aattgcttca tatcttgctt ttttgtctcg aatgcgctct
    17641 ggcagttcag gaaacagacc atagttggca ttcatcggtt ggaaatgttt tccttccgca
    17701 tgagtaatgt agtaagccat acttccaata gctgtttctt gtggaaactc gactggttcc
    17761 atttcttttg ctaagcgtgc ggcgttgatt ccagcaagta gtccgctcgc tgcactttca
    17821 acatagcctt ctacaccagt catctgacct gcaaagaaca gatcttcgcg tttcgttgat
    17881 tggtaagtcg gtttcagcaa ttctggcgaa ttcataaagc tgtttcggtg catcacacca
    17941 tagcgaacga attcagcatt ttctaaccca ggaatcatcc ggaaaacacg tttttgctcg
    18001 ccccatttca agtgagtctg aaagcccacg atgttgtata aagaagctgc agcattgtct
    18061 tgacgcagct ggataacagc ataaggacgt ttgccagttt taggatcttc caatccaact
    18121 ggtttcatag gtccgaaaag catcgttttg aatcctcgct gtgccattac ttcaattggc
    18181 atacatcctt caaaatattt ttctttctca aaagatttaa gttctgccac ttcggcattg
    18241 attagtgcct catggaaagc tttgaattcc tcttctgtca tcggacagtt caagtaagct
    18301 gcttctcctt tgtcatagcg ggacttcaaa tatactttat ccatatcgat cgtagctttg
    18361 tctacgattg gtgcagctgc atcatagaaa taaaaaccat ctgaaccatt gaatgctttg
    18421 atgctttctg ctaatggatg agaagtcaaa ggtcctgttg ctactacggt gattccttcc
    18481 ggtatttcag taatttcttc gtttatcact gttactaatg gatgctcttt gacttttcgg
    18541 gtaattgttt cagaaaaaga atcgcggtca acagctaagg caccaccagc tggcactgct
    18601 gtttcatctg ccgaagtgat tactaccgaa ttcaatcggc gcatttcttc tttcaataac
    18661 cctactgcat tagccaaact gtttcctctt aatgaattgg aacatactag ttcagcaaaa
    18721 ttacttgtgt gatgcgcctc agtcgatttg actggacgca tttcatataa acggacaggg
    18781 actcctgctt gtgcgatctg ccaagcagct tcacttccgg caagccctgc tccaacaaca
    18841 tttaccattt tcaatgaaag aacctctttt ctattatttt tggatgtctt cttcgtaatc
    18901 tccatttaca cagactactt gtttaccgcc tttgactttc ttctcgacca gatatccgtc
    18961 acattttgga caatttctgc ccactggttt atcccaagaa gtgaattcac attctgggaa
    19021 acgggaacaa ccatagaata gtctgttttt cttagatttt cgttcaatga cttgtccttc
    19081 atgacatact gggcaagtca caccgatttc ttttacgatt ggtttcgtat tccgacaatc
    19141 aggaaaatta ctgcaagcat aaaacttccc atatttccct aattttatca ccatcggatg
    19201 tccacagaca tcacaatcga atcctgctgg ttcatctttg atttgaattt tttcgatttt
    19261 ttcttccgca ttcgtcagtt ctttttcaaa cggtttatag aaacgatcaa ccactttgac
    19321 ccacgcttcc gtgccttctt caattttatc caaatctcct tccatttctg ctgtgaaatg
    19381 gatatcgacg atttgaggga agaattcaca gattaatgaa ttgacaattt ctcctagttc
    19441 agtcggctca aatctttttt gtgtcaattt tacatagtag cgtctttgga tcgtatcaag
    19501 tgttggtgca taagtggaag gtcgccctac cccgttttct tctagtgcac gaatcaacgt
    19561 agcctcacta aaacgtgctg gtggttgagt aaaatgttgt tttggttcaa tatcaacaga
    19621 ttggactaca tcgccttctt caagttctgg aagaatattt tctttctctt ctttgccgtc
    19681 atctctacct tccacataca cttgcatgaa tcctttgaat ttgatttttg aaccgttcgc
    19741 aatgaagatg acaccatttt gttctaatgt cactttcatt gtatctaaaa tagctggtgt
    19801 catctgacta gccacaaaac gtgaccagat caatgtataa agtttcaatt gatctttatc
    19861 taaatattgt ttgagttcat ctggtgtacg aagcacactc gttggacgca cagcttcatg
    19921 ggcatcttgt gcgccttgag catttttgat ctttcggtga tcatgtctag aaaattcttt
    19981 tccatacgta tcttcaataa atgctgcggc ctctgctttt gctgtatcgg caatccgggt
    20041 agaatcagta cgcatgtatg taatcaagcc gactgttcct tgtttaccca aagtgattcc
    20101 ttcatataat tgctgtgcta ccatcatggt ttttcttgtt cgaaagttta acttacgggc
    20161 agcttcttgt tgcaggctac ttgtagtgaa aggattcgca gggtttcttt tgcgctcttt
    20221 cttttctact tttttaactt cgaagtctgt tccatcaatt ctagcagtga cttcttttac
    20281 actttgtgca tctggtaatt tctttttctt accgtcgatt ccccagaaat ttgctttgaa
    20341 tttcttgcga gcttttttaa aattaccgtc gatgctccaa tattcttctg gaacaaattc
    20401 tcggatctct ttttcacggt caataataat ttttaaagcg acagattgga cacgtcctgc
    20461 acttaaccct ttcttgacct tacgccagag aataggactg atcgaataac caacgattct
    20521 gtctaaggta cgacgagctt gctgtgcatc tactaaatct acatcgatcg ttcttggttc
    20581 cttaaaagct gctttgactg cgtcttttgt gatttcattg aagaccaccc gatttttttc
    20641 tttcagatcc aacccaagta ggtacgctaa atgccaagca atcgcttctc cttctctatc
    20701 cgggtcactt gccaagtaaa ctttttgagc tttttttgct gcggctttca ggcttttgat
    20761 gacatcgcct tttccgcgta tagaaatata atggggttca taattgtttt caaaatcgat
    20821 acccatttta cttttcggta aatctcttat atggccaaca ctggccatta ctttatagtt
    20881 tcgtccaaga tatttctcaa ttgtcttggc ttttgcaggt gattctacaa tcactaaata
    20941 tttatacgcc attactgaat ggactccttt ccattgtcgt gttcctacta ttaaaagagg
    21001 attcatacaa atcgtagcta atcatatcca ttcagaaaac gtttgtcaag aaaagcaaat
    21061 gaaaagtatg gtgttattct ttaaattctg ccaagatatc ttcacccgaa accgtgcaaa
    21121 ctgcaccgtc ttggatcaaa tgatgacatc cggtagaatg ttctgttaaa atatttcctg
    21181 gaacagaaaa aactgttctt ccaagatcca atgcttgctg tgctgttatc agagagccgc
    21241 tttttttctt ggcttcaaag acacatgttc ctttagacaa agctgcgata atccgatttc
    21301 tcattggaaa acaatgcttt ttcgggccgg attgaggagg atattcactc aaaagaagat
    21361 gatcatttgc aatcacctct tgcaagtcac gattttcagc aggatagact tgctctatcc
    21421 ctgttccaat gactgcgatt gtccgtccac catgagagat tgctgcttgg tgaccgtatg
    21481 tatctatccc tttcgcgcag ccactgacga tcacatatcc agcttctatc actggtttta
    21541 gtaaataata aactgttttt ttggctaaag cagaagtgtg tcttccacca acaacgctta
    21601 acatgttaca agaaagtaaa gatagatcac cattataaaa cagtacaatt ggcggatagg
    21661 tcaagtgaag cagctgaggg ggatatttgg gatcaaagca agtgataaac gactgtttcg
    21721 attggatttt gccccagtta gcgtgaattt cctgccaact tttgcgaaac agctcccggt
    21781 gtttggttat tttacctatc ttaatgatgt cttcaaatcc gatttcttta taaggatact
    21841 gcatcatcca gttagcaagt gcccattttc cttgactact gattcctttt gtataagcaa
    21901 gtttcaataa attttcttct atttgataca tcataaaacc gcctttcgtc tctctttcta
    21961 ataagatacg taaaagcggc tgttttttat tgtaaatatt tttttacagg tgcaaaactt
    22021 ttacgatgga ttggcgtaat tccttgtcgt tcaagtcctt ctaagtgttc ctttgttcca
    22081 taacctgcat tatgcgagaa accatatcca ggataaagca cgtcatattc tttcatcaat
    22141 tggtctcgat atactttagc aatgatactt gcagcagcaa tcgacacaga tcgtgcatcc
    22201 cctttgatta ttttttcctg ctgaataggc aaatccaagg tcatcgcatc gatcaacaaa
    22261 tagcttggta caatggacaa tttttctact gcttggatca tcgctaattt acttgcttca
    22321 taaatattca catcatcgat tgtctgctga tcaacgatcc cgatcccgat tgccagggct
    22381 ttttcttgaa cttgttgata aagaagttct cgctttttct ctgataattg tttggagtca
    22441 ttcagcccta atatgatctc atttttcggc aagataactg cagctgccac gacaggtcca
    22501 gctaatggtc ctcttcctac ttcatcaatg ccggcaatcc attggtgacc ttgtgcgtac
    22561 ccttgctttt caaagcaaaa catttcttca tatttttcct taattaatgc ttcctttttc
    22621 aatcgtcgtt caaaagaagc cagtgcttgc tgcacgcctt tgcgtggatc atttcttaat
    22681 gattctagtg taggatcatc tattctctgt atgcttttca atcgttcttt gatactttgg
    22741 atggattcag tcatcacttt tttgctccat ttcttcgcaa cgatccagtg tgtatcgtcc
    22801 aagttttcct tgacgaatat catgcacgac taattcgctg ccacgatcat agtcttcttt
    22861 atacccacgt tttgctgtaa tatccatcag taattccggt gccaattttt cttcttgctc
    22921 gattgataaa ccataacgat cgctgattct tcctgaataa tatcgggcaa aaaagtctaa
    22981 tccgtaaatc gctaaatcat ctaaatggac aagctggtct ttgatcgcgc ctgtcagcgc
    23041 tagttttttg ccgatttctt catcttcaaa tttgggccat aaaatccctg gtgtgtctaa
    23101 caattccaac tcattaccta aacgaagcca ctgctgacct tttgttacgc caggcttatt
    23161 tcctgtctgt gcaattttct tgcctactag tcgattcatc aacgtagatt tcccaacatt
    23221 tggaatacct aagaccattg cccgaatagc gcgaggcttg atcccccgag cagcatctct
    23281 tgcccgtttt tctttcaatg cttctttagc cgcaggtact agacctttaa ttcctttact
    23341 ttcttgtgcc gtgatagcta atgcaagatg gccttgttta cgaaaatatt cttgccacag
    23401 ctttgtctgt tgtggatctg ccagatccgc tttattcaat aaaacaaggc gaggtttttg
    23461 ctgaatgatc tgatctagca ttggattacg agaagataaa ggtaatcgtg cgtctaccaa
    23521 ttcaaatacg atgtccacat atttcatttt ttcagaaact tctcgtcggg cttttgccat
    23581 atgtcccgga aaccattgga tggtcattgt tctctccatt ccttt
//
LOCUS       DAYYAR010000034        23522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000034 DAYYAR010000000
VERSION     DAYYAR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.034"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..1821
                     /locus_tag="ACHAT0_001148"
     CDS             160..1821
                     /locus_tag="ACHAT0_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8451103.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1891..2238)
                     /locus_tag="ACHAT0_001149"
     CDS             complement(1891..2238)
                     /locus_tag="ACHAT0_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8451104.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2322..3263
                     /gene="opp3b"
                     /locus_tag="ACHAT0_001150"
     CDS             2322..3263
                     /gene="opp3b"
                     /locus_tag="ACHAT0_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8451105.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3264..4307
                     /locus_tag="ACHAT0_001151"
     CDS             3264..4307
                     /locus_tag="ACHAT0_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8451106.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4313..5383
                     /locus_tag="ACHAT0_001152"
     CDS             4313..5383
                     /locus_tag="ACHAT0_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8451107.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5373..6326
                     /locus_tag="ACHAT0_001153"
     CDS             5373..6326
                     /locus_tag="ACHAT0_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8451108.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6338..7348
                     /locus_tag="ACHAT0_001154"
     CDS             6338..7348
                     /locus_tag="ACHAT0_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8451109.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7633..8454)
                     /locus_tag="ACHAT0_001155"
     CDS             complement(7633..8454)
                     /locus_tag="ACHAT0_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8451110.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8551..8829)
                     /locus_tag="ACHAT0_001156"
     CDS             complement(8551..8829)
                     /locus_tag="ACHAT0_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451111.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9097..11106
                     /gene="metG"
                     /locus_tag="ACHAT0_001157"
     CDS             9097..11106
                     /gene="metG"
                     /locus_tag="ACHAT0_001157"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8451112.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11585..12172)
                     /locus_tag="ACHAT0_001158"
     CDS             complement(11585..12172)
                     /locus_tag="ACHAT0_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451113.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12347..13669
                     /locus_tag="ACHAT0_001159"
     CDS             12347..13669
                     /locus_tag="ACHAT0_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8451114.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13824..14600
                     /locus_tag="ACHAT0_001160"
     CDS             13824..14600
                     /locus_tag="ACHAT0_001160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8451115.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14593..15162
                     /gene="rnmV"
                     /locus_tag="ACHAT0_001161"
     CDS             14593..15162
                     /gene="rnmV"
                     /locus_tag="ACHAT0_001161"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8451116.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15155..16039
                     /gene="rsmA"
                     /locus_tag="ACHAT0_001162"
     CDS             15155..16039
                     /gene="rsmA"
                     /locus_tag="ACHAT0_001162"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8451117.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16490..17119
                     /locus_tag="ACHAT0_001163"
     CDS             16490..17119
                     /locus_tag="ACHAT0_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451118.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17243..18352)
                     /locus_tag="ACHAT0_001164"
     CDS             complement(17243..18352)
                     /locus_tag="ACHAT0_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8451119.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18561..18983
                     /gene="mgsA"
                     /locus_tag="ACHAT0_001165"
     CDS             18561..18983
                     /gene="mgsA"
                     /locus_tag="ACHAT0_001165"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8451120.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19170..19268
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19306..19842
                     /locus_tag="ACHAT0_001166"
     CDS             19306..19842
                     /locus_tag="ACHAT0_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8451121.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19882..21645)
                     /locus_tag="ACHAT0_001167"
     CDS             complement(19882..21645)
                     /locus_tag="ACHAT0_001167"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8451122.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21648..23363)
                     /locus_tag="ACHAT0_001168"
     CDS             complement(21648..23363)
                     /locus_tag="ACHAT0_001168"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8451123.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7584 a         4303 c         4776 g         6859 t
ORIGIN      
        1 tattttttct gaaaaattcc ttgaaacctc acgaaaaaat cattatttta tgaaaaagac
       61 aaaaaaaagt gttgattttt taataattat tcattaaact cttaaacaac ttcagaaaat
      121 attaattatt ctgaaaattc caataaaggg gtacgtaaaa tgaaaaagaa atttacattt
      181 ggtttggttg cggtatgcgg ccttgtactt gctggatgtt atggcggcag ttcagccaca
      241 agcagtagtt cagcaaaaag cggttcagca gatggaggag gcgtatttaa cttagtcgtg
      301 cctcaagaaa tgcctacagc tgacttatca gttgcaacag atacaatcag ttttacagca
      361 ttgaataacg tttatgaagg aatttaccgt cttgatgaag acagcaaacc acagccggca
      421 ggagctagcg agttggcaga agtcagtgaa gacggattaa cttacaaaat caaattacga
      481 gaagacgcaa aatggtcaaa tggtgatcct gttacagcag cggattacgt ttatggctgg
      541 caacgaacag tagacccagc tacagcttct gaatatgctt acctgtatgc gccagttgaa
      601 aatgcagaag acattacagc tggaaagaaa gataaatcag aactagggat caaagcagtt
      661 ggtgactatg aattagaaat caaattgacg aaacaaacac catatttcca atatctgctt
      721 gctttcccat catttttccc acaaaaacaa tccgtagtgg aagaaaatgg ggatgcctat
      781 gcatctgcaa gtgataaagc tgtttataat ggaccattca cattagaaga ttttgatgga
      841 ccaggaacag atacagaatg gacttataag aaaaatgatc attactggga caaagataca
      901 gtaaaacttt ctgaaatcaa agtcagtgta gtaaaagaat cttctactgc attgaactta
      961 ttcaaagatg gccaagcaga tgacgtgatc ttgtctggtg agttagcaca gcaaaacgca
     1021 aacgatccag cttatacttc tgttaaagaa gcgcggacaa gctatatcga attcaatcaa
     1081 cgtgaaaaga attcaccatt caacaatgtg aacctaagaa aagctatttc ttattctatc
     1141 gatcgtgaag ctttagtaaa acaagtacta ggtgacggat cagttgtctc tacaggattg
     1201 atcccatcag atatggtaaa aagtccaaca acgaacgaag actttgcaaa agaagctgga
     1261 aaattagttt cttatgacaa agataaagca aaagaatatt gggaaaaagc gaaaaaagaa
     1321 ttgggcgttg attctttaga atttgatttg atggcttctg atgatgattc atcgaaaaaa
     1381 gtaatcgaat atgttcaaaa ctcaatccaa gaaaacttgg atggcgtaac agtcaaacca
     1441 acacctgttc cattctctgt acgtcttgac cgctcaactt cgggtgattt tgacgcagta
     1501 ctaggcggat gggctgctga ctatgcagac ccaagcagct ttactgattt gtttgtcaca
     1561 ggtaattcat acaaccgtgg tcgctggagc aatgcagaat atgacgaagc agttaaagca
     1621 gcttcatcaa cagacgctgg cgatgaacaa gcccgttggg atgatttcca aaaagcaatc
     1681 aagatcatct ctgatgatat gggtgtcgtt ccggtgtacc aaaaagcaga aggtcaccta
     1741 gtgaatgaaa aaatcaaagg tatcgttcac catgcggcag gcgcttcatg ggattacaaa
     1801 tggacatacg ttgaagaata atcgttaata ccgtgagcat gagaaaaaac gaggctgcgg
     1861 caaatgtcgc aacctcgttc tcatattcac ttatcctaac aaagaaaaaa gcatgaaaat
     1921 agcagaaaga gcaattagta ttccggcaat gatcagccaa aagctgtaca taccgtagct
     1981 ggcagacgag agcgtggaaa attctggttt tttctttcgg ttccttgttc gtgcaagatg
     2041 gaccgatcgc tgaaaatgat cgaagaaacc cgaagaaaag acccataaaa aaccgccgat
     2101 aatcagaaac ggcaatgacc ataagaatag atcattagat aattgaatga gtgtaaagga
     2161 atggttgatg agatttttta ttaaaataac cagaatcagt agaaaagcta ctgcgtaggg
     2221 gatagatttt tttttcattt gattgctcct ttatttttgg ttcattatct aataatgtag
     2281 cgaaacatct gtcagaagtc aaaagctagg gggaaacatt agtgaatagt tatatcaaat
     2341 atgtattaaa acgtgtcttt ttcatgatca tcacgctttg gttaattgca acaatcacct
     2401 ttttcttgat gcaattgctg ccgggcacgc catatacgaa tcaggaacgt ttgagtcctg
     2461 aaacaatcga gatgctaaat aaacaagtcg gtttggataa accagttatc gtacaatatg
     2521 ggatttatct ttctaatttg cttcaaggag attttggaat ttccttccaa tttaaaaacc
     2581 agccagtcgc acatttgtta gctggaagaa tcggaccatc cttgcagtta gggttacagg
     2641 caattatctt tggaacagtc ctagggacga ttttaggaac gatctctgcc atgaagcaaa
     2701 atacatgggc agatacatct tctacactcg tagcgatatt aggtcgttcc attccaaact
     2761 ttgtatttgc tgttttatta caatatatct ttgcaatcaa attacaagtt ttgccaatcg
     2821 caaaatggga tggctttgtg tacacaatct taccaacaat tgcgttggcc atgtcgccgc
     2881 ttgctgactc tgctcggttt atccgtacgg aaatggtgga agtactgcat agtgattatg
     2941 tggaattagc aaaagcaaaa ggattgagcc gttgggaaat cgcctttaaa catggtcttc
     3001 gaaatagttt gattccattg atgactttat taggaccgtt agctgtagca ttgatgaccg
     3061 gatcactggt ggtagaaaat atctttgcta ttcctggtat cggggaacaa ttcgttaagt
     3121 caatcacaac aaatgattat cctacgatca tggctgtaac gatcctttac tcatttatgc
     3181 tgatttttgt tattttgatc gtggatctat tgtacggtct agttgatcca agaattcgtg
     3241 tttcagaagg gagccgagga taaatggaaa tgataccaaa gcgttatgaa acagttgcag
     3301 atatccctgc ggatgaattt cttccattac aaaaaaatac cgaagaagaa cgtgaacaga
     3361 ttactgcacc ttctttgaat tttatccaag attcatggcg ccgtttaaag aaaaacaaag
     3421 ctgccgttat ttcaatggtt ttactattgg tcatcatttt gatctctgtg atcacaatct
     3481 ttgtttctcc tcatgatcca actgcccaaa acgtggctta tatcaatttg cctcctcgta
     3541 ttccgggaat caatatcgat ggattgaatg gaaaagcaat ggttggtggt gaacttgttg
     3601 ataaatatgc tcaagcaaac gtcccttcaa acgtaaattt ctttttagga acagacggtt
     3661 tgggacgtga tgtattaagc cgcttattta tgggaatgag aatctcgttg ctgatcgcat
     3721 ttattgctgc attactagat gtaacgattg gtgtagctta cgggttggtt tctggtttgt
     3781 taggcggacg tgtcgataac gtgatgcaac gttttttgga aatcttatca ggaattccaa
     3841 acctcgttgt catgattttg atgttggtcg tttttgaacc aggaattttt tcaatcgtgg
     3901 ctgctatggc catcacaaac tggataccaa tggcacgtat cgttcgtgca caaacaatga
     3961 aattaaaaga tcaagaatat gttttagctg gattgacatt aggtgaatca agaatgaaaa
     4021 ttgcattcaa acatattttg ccgaatatct ctagtgtcat tattattcaa atgatgttta
     4081 gtattccatc tgctattttc ttcgaagcct ttttaagctt tatcggttta ggtcttacgc
     4141 ctccgtcagc ttcattagga acgatgttga gcgaagggta caagacattc ctgtatctgc
     4201 cttatctatt atggattccg gcagtaactt tatcggttat catgattggc tttaacttac
     4261 ttgctgatgg attgcgtgat gcctttgatc ctaaaatgaa agagtgaaga cgatgacgaa
     4321 aattttagaa gtgaaagact tagaaatctc ttttgatacg tatgctggaa aggtacgagc
     4381 gattcgcggt gtcaattttc atttgaataa aggagaaaca ttggcgatcg ttggggaatc
     4441 tggaagtgga aaatcagtga cgaccagaag catcatgcgc ttgctgtcaa gcaacgccaa
     4501 tatagatgct ggtcaaatcc tctttaaagg taacgatatc gttggaaaat cagaaagaga
     4561 gatgcaaaaa atccgtggta aagaaatagc gatgattttc caagatccaa tgacctcttt
     4621 agatccgacg atgcccattg ggaagcaagt cgctgagtcc cttagaaagc ataataaagt
     4681 ttcaaaaaaa gagggacaaa aagctgcatt agaattgctt aaattagttg gaatacccga
     4741 agctgagaaa cgtatcaaca gttatccgca tcagttctca ggaggtcagc gtcaacggat
     4801 cgtgatcgcg attgcactgg tatgttatcc ggaaatcctg attgctgatg aacctacaac
     4861 agcgcttgat gtgactattc aagcacagat tttggagctg ctgaaagaaa ttcagacaaa
     4921 aatcgataca tcaattatct ttatcactca tgaccttggt gtagtagcga atgtcgctga
     4981 tcgtgtcgct gtcatgtatg gaggaagaat cgttgaagtt ggaacatcgg aagaaatttt
     5041 ctacaacccg caacatccct atacttgggg actgcttggt tcaatgccga cattagatag
     5101 tgcgaacgat cgattgtatg ctattcctgg ttctccaccg gatttattga acccgccaaa
     5161 aggagacgca ttctatccaa gaaatgaatt cgcgatgaaa atcgatgcag aacaagaacc
     5221 accatttttt gaactatcaa aaacacacaa ggcagcaact tggctacttg ctccgcaggc
     5281 acccaaagtg acaccgccag aagaaatcca acgtcgctgg gctatcttta aagagaaaca
     5341 aaaacatact tacggtcaag gaggaattgc tgatgccaaa taaactactt gaagttaaag
     5401 gattgaaaca gtatttcaat gtaggaaaaa agaatgaagt acatgctgta aatgacatca
     5461 gctttcacat ttatgaagga gaaacattcg gtttagttgg agaatctgga agcggaaaat
     5521 ccactactgg ccgtacgatc attcgcttga atgaacctac tgatggtgag atattattcg
     5581 acggtcaaga tataacgaag atcaaagata aaaaaggttt gacgaaattc cggcatgatg
     5641 ttcagatgat ctttcaagat ccatacgctt cgttgaatcc tcgtatgaaa gtcagagata
     5701 tcattgccga aggaattgat gttaatggac tagctaaatc atctgaagag cgtgcaaaaa
     5761 aagtcgatga tctattgaga actgttggat tgaatccaag ccacggtact cgttatcctc
     5821 atgaattttc aggtgggcaa cgccagcgta ttggtattgc acgagcattg gcagtaaaac
     5881 cgcgttttat catttgtgat gaaccaattt ctgcactaga tgtgtcgatc caagcccaag
     5941 tggtcaattt gttgcaagat ctccaaaaag aacatcaatt gacctatcta tttattgcac
     6001 atgatttgtc gatggttaaa catattagtg accgaatcgg tgtaatgcat aacggcttgt
     6061 tattagagat gggaacaagt gatgaaatct ataatcacgg tgttcatcct tatacagaaa
     6121 gtttgttatc tgctattcct ctaccagatc cagaccatga gagaaaacgc agaagaatca
     6181 aatatcaacc tgaaccagat gacggacaag tacgtcaact tcgtgaaatc gcaccagaac
     6241 attttgtcta tgcaactgag caggaagtcc catattacgc aaaaaaacta aaacgacaaa
     6301 aggaatctct tctggttgct aactaggagg gacatagatg ctaaaagctt ttaaattccg
     6361 tatgtatccg acagaagaac aaaaacagca attgattcgt acttttggat gtgctcgatt
     6421 tacctataac catttgctga aaaagcggca gaaaagttgg caacaaacag gagtagccaa
     6481 tttttcgctc acacccgcta cattaaaaaa agaataccca ttcttaaaag aagtagacag
     6541 tcttgcttta gcaaatgcac agcttaattt ggatcgggct tttcgcaatt atttcaaggg
     6601 acgtgccagc ttcccaaagc ttaaaacgaa aaaaagtatg tggcaatctt atacgaccaa
     6661 caaccaaacc cgtacgatct atctggaaaa tggctactta aagttaccaa aacaaaaaga
     6721 actaatcaag ataaatcagc atcggccagt tgaaggatcg attcgctccg ctacgatttc
     6781 tgctcgatat aatgaagaat tttatgtcgc actactttgt gatgtttcgc cagtaaaaaa
     6841 agaatcttta gctaaatgga tcgggatcgc ctatcatccg aagacattga tccaaacatc
     6901 tcgacctcta gaagtaactt taccaaaatt tcaccagaca gaagaaaagc tgcaacatgc
     6961 acagcggaaa ttgaatgtga aagtccgctc cgctcaccat agaaagatca ggttagatca
     7021 ggcaagcaat tatcagaaac aaaagagaaa agtaatggat ctttatctga aacaaaagaa
     7081 ccagcgagaa gattatcttg aacaattatc tgggaaattg gtcaaacaat atgactactt
     7141 atttgtagaa tcttttccaa aagaagaggc acatgctgat ttttctattc acgactggca
     7201 taaattaata acaaaattgc gttataagtc ccaatggtat aataaaaaat ttcttttgat
     7261 caataccgac ggagcagaag aaagcaattc cgtaagaaag agccaagtag tagaaaaaat
     7321 gggcagacat tcggttatca agggataaaa aatgaatgaa actgatagga gcgttagggt
     7381 tagccttgtt aataaaaagt cttaatgact tttgtttcta ggaatttttc ttttgaagaa
     7441 aagctcacta attatgacaa accaataaaa caatagcaga aaaagttcgt acgagctttt
     7501 tctgctattg ttaatttttt attcagaaag tttacgaata catcaaaatt ctgaaacaaa
     7561 aaacgtaact cacaaagagt tgtttttgcg ttttttcttg aatgattagg tctgaatctt
     7621 ttgctcacaa ccttattcat ggtgccccgg cttgagctga tagatttttg aaggacggcc
     7681 gattcctaca ggacgttcac cgatctcatc gaaatattgg agcatagctt tcttaaaatt
     7741 ggaatgatcg atcgttttat aatcgatccc taagaattta gcaaatactt ttcgagcttc
     7801 cgtaatcgtg aaatctttac ctaaaacttg tagaacttgc ggctcgtgtt ccattttatt
     7861 gactacacgg ttgaatgctt tcaagatgat ttgactatgg tcaaaagcca aagtatcttt
     7921 gccagaagac tctccagtga ctaaatcaag cgagatagcc acttctccgc tagataaatg
     7981 gatcttgtta ctatgacgtt ctagtgtgaa ccagcgtact tccttggcat catctccggc
     8041 gattagcggt tcttctccaa taaaggctaa gtagctgact gtgacgaccc agcctcgcgg
     8101 atctcgatta ggcgtactga atgtatgcag ttgctcaata ttttcttttg aaataaccac
     8161 accagtttct tcctttgttt ctcttaagac gctttctcca gttgactcat ccttttggac
     8221 aaagccacca ggcaatgccc atgaatttcg ataaggatgc ccttttcttt ggatcaacaa
     8281 aactttcagc tgatcttctt ctttgttata gcacatcagt acgatatcga cagtcaaaga
     8341 gggcttgtca tattctggac gttcctgttt ttgataccag ttaagaaatt cttcttcact
     8401 tgctttatgt tcataatata atttttcttc ttctttagaa gcaaaacggt tcatctgacc
     8461 acctccattg acagtaaaag tactttttac cttattgtag cagactctga aaaaaaacca
     8521 aggatttact tgaaaaatct aggttatcat ttaaatatga aaaataaatg gaagtatgag
     8581 attgaataaa aagaaaagca gataaaacgg caatacttta taccgctctt cacgaatggc
     8641 aaagaagcca caaataacga tatatacgtc aatgataaaa cgcaaaatcc aagctgctgt
     8701 cataaaactg ttcaaatgcc catggaggtt aaaataacca tacatacgaa tataaaaata
     8761 gctgaatatt aaatgtgcta atgaaagaaa agcaatacta agtgtaaaaa aaagatgttt
     8821 cttctgcatg attgttccag tcccttacta agatagatag tctttattat accacgtgtg
     8881 aggggagaaa taaaaagccg ttttttagta gatatgaaac aaaacaaaaa cccgcattta
     8941 ttcaacctat tcatcctttt aaaggataga ttgaacgtag atagaagtcg tttatccagt
     9001 ctttttcatt ttgaaaaaaa gctaacagca tgcttttttt atggtatact agcaaggaaa
     9061 cgaattcgca tcatgcgata aaggaggaag agccccgtgg cagaaaagga aactttttat
     9121 atcactacgc caatctatta tccaagcggc aaacttcata tcggcaactc gtatactacg
     9181 attgcatgtg acgcaattgc tcgttataaa cggttgatgg gctttgatgt gttttatctc
     9241 actggagtgg atgaacatgg tcaaaaaatc gaaacaaaag cagaagaact aggtgtaaaa
     9301 ccgcaagaat atgtagataa aatggcggct gatgtcaaga aattatggaa aacgcttgat
     9361 atcagttatg ataaattcat tcgcacgaca gatgattacc ataaagcagc tgttcaaaaa
     9421 atatttgatc gtttattaga acagggagat atttacttag gtgaatatga aggctggtac
     9481 tctgtttctg acgaagaatt ctttacagaa acacaacttg cagaagtgta caaagatgac
     9541 gaaggtaacg tgatcggtgg taaagcgcca agtggacacg aagtagaatt ggtaaaagaa
     9601 gaatcatact tcttccgcat gagcaaatac gcagaccgtc tattggaata ctatgaagaa
     9661 catcccgaat tcatccagcc agaatctcgt aaaaacgaaa tgatcaacaa ctttatcaaa
     9721 ccaggattag aggatcttgc agtttcacga acaactttta cttggggagt accagtcaag
     9781 agtgatccga aacatgtggt gtatgtatgg atcgatgcat tatccaatta tattactgca
     9841 ctaggatatg gctccgaaga tgaaagtcta ttcgaaaaat actggcctgc agatgttcag
     9901 atggtcggta aagaaatcgt tcgctttcat acgatctatt ggccaatcat gctgatggcc
     9961 cttgatttgc ctctacctaa aaaagtattt gggcatggct ggctattgat gaaagacgga
    10021 aaaatgtcta aatcaaaagg aaatgtcgtt tatcctgaaa tgcttgtgga acgttatggt
    10081 ttagatgcac tccgttatta tttgcttcgt gctgttccgt ttgggtcaga tggtgtcttt
    10141 acgccagaag attttgtttc ccgtgtcaat tatgatctgg caaatgatct agggaactta
    10201 ttgaatcgaa cgattgctat gatcaacaaa tattgtgatg gactcgttcc tgactatgcc
    10261 tcacttgtca ctccatttga tagtgaactg tctacgacgg ctgctaacgt tgtcggacgt
    10321 taccatgatg caatggaaaa aatggaattc aatacagcat tggctgaaat ctgggtgctg
    10381 atttctcgtg caaacaaata cattgatgaa acagaaccat gggtattagc aaaagacgaa
    10441 gaaaagaaag ctgaattgaa tagtgtcatg atccatttag ctgaatcttt gcgtatcgca
    10501 gcgatattac ttcagccaat catgacagaa acgccagaga agatcttcaa tcaactagga
    10561 ttggactctg aaacaatgaa tcttgaagga ttacactttg gcgaatttcc ttcaggaaca
    10621 aaagtagttg caaaaggtac accaatcttc ccacgtttag atatggaaga agaagttgct
    10681 tttattcaag aaaaaatgtc tgaaggtact caaacaaacg aagatacagt caaatgggat
    10741 ccagaagaaa cagaacttgt ttcaacgaaa gaaaaacaaa tcaaatttga tgtatttgaa
    10801 aaagtagaac taaaagtagc agaagtcatc aactgtcaaa aagtagaagg ggcagataag
    10861 ttgctgcaat tccgactaga tgctggagac agccaagacc gtcaaatctt gtcaggaatc
    10921 gctgaatttt atcctgatcc aagcgaattg atcgggaaaa aagtcgtcat tgtagcaaat
    10981 ctgaaaccac gaaagatgcg tggacaaatc agtcaaggaa tgatcctttc tgcagaagca
    11041 ccagatggct ctttacaagt aatcgaagca ccaaaatcca tgccaaatgg ctcagaaatc
    11101 gcatagaaat tcaaaggggg tatgacacta gttgtgtcat accccctttt tccgaaattt
    11161 gagaagcaat tttcgtaaat tccttcttat tacttgaagc caatcatata atacagaaac
    11221 agagaactat ccaaaataaa tcctagaaaa agaagtacga gcttcgctga tttttgttaa
    11281 tttagaataa tactaatagg aatcgtaaaa acagaaaaaa agcataatga taaagggtcg
    11341 ggcagatttt aattgattca tctggtactc ttttttattg tccttacatt cataaagttt
    11401 gaatgcaatg actttattac atctatattt atatctaaca gtagcgttga tgtgttaatt
    11461 ataagttcta tcactttatg ttcaattagt ttgtgctctt atatgtgtga ttgaaaatat
    11521 agataaaaaa acaatcatcg gctttgactt cgatgattgt tttctttatt ttgtaaaaat
    11581 cctatcactt gattttatgg taacctttta tttttgtctt caaacggctt ggtaagagag
    11641 cagtaagcag tgcaaatggg atcgaaaaga cataccagaa aatcgtacgt ccgataccat
    11701 aatgttgata gagctttgtc aacatcacgt gtaacaagat caaccctaaa agcaaggagc
    11761 cagctataaa ccaatgatta tcccaatgca tgacttcact aagtgccatc actccaaaag
    11821 gatagaaata aaaatagatc ccgactaagg tcatccgcca gttctttgta aatgttccga
    11881 tattcaataa aaagaaaatc gctgaaagca aaataccgat aggcggaaaa agcccaacca
    11941 aaaaggaata caacatcccc cacaagatca tcacaggacc tttgatcaat gcaacgattg
    12001 cgatgaacaa agtcagcaat attagttttt gtgataacgt tgcaacagtc attaatccaa
    12061 ataaaattaa tgtgaaccaa tgccaatggg actgctgttg ttctttacgg gaaaaatcga
    12121 cccggttgga taaataatct gctaatttcg gataagaacg cttaaaatcc ataaactgtt
    12181 cctccttcgt ggaaaaggta ggtttagtat acgaaaaata aaaaagaaat acatccgtct
    12241 taagagggag tttttcactg aaagtggaga aaaaagaatg gtaaattaaa atattctcag
    12301 ttatactgaa aattatacca aatttcaaag aagaaagagg atgggaatga aaggcaaact
    12361 aaaacgagaa atcaatctgt ttggcgcatt agctaccgtt atgggaaccg tgatcggtgc
    12421 aggcgtcttc ttcaaaactg ctgctgttac tgctagtact cagtctacaa gtttgacatt
    12481 gctagcttgg cttttgggtg gtttcttaac gatctgtgct ggattgacag tggctgaatt
    12541 agctacagca atcccggaaa ctggtggagc tgtaaaatat attgaagctg cgtatggaaa
    12601 attgcccagc tttttattag gatgggcgca aagcttgatt tactttcctg ctaatattgc
    12661 tgcattatcg attatctttg cgacacaatt gacgaactta ttacaattat ctaccgatta
    12721 tcttttgttg attgcagtga tcaccgctgt ttctgtgaca gggttgaatt tgttagggac
    12781 aaaagtcggt gcttctgtac aatcagtgac gttgattgtt aaattaatac caattgctgt
    12841 gattgtcata tggggattat tgaccccagg acaaggaact gtccagttat ttccaatcga
    12901 agctggaaaa gatgttactt ttgtcgaagg attgagcagt gcgttgttag caacgttgtt
    12961 tgcttatgac ggctggttag gtgtcggtgc aatggcaggg gaaatgaaac ggccggaaaa
    13021 agatttgcct aaagcaatta ttcttggttt gagttttgta acagtcgttt atctattgat
    13081 caattttgtt tttttgaaaa ctttgccgat tgatcatctt gccggtaatt tgaatgcagc
    13141 ttctgaagca tcagacgtca tttttggagg aatcggtgga aagctggtca ctattgggat
    13201 cttgatctcg gtatacggtg cattgaacgg ttatacacta actgggatcc gcgtgcctta
    13261 tgcgatggct ttagaagacg agctaccttt cagtaaacag ctgacaaagc tttcaaagaa
    13321 gtttacggtt ccttatgttc ctgcggtttt ccagttagca gtagcttgta ttatgatgag
    13381 ccttggttct ttcgactttt taacagacat gttgattttt gtgatgtggc tattcagttt
    13441 actgattttt atcggtgtct ttattttaag aaaaaaagcg ccggaattgc cacgtccata
    13501 taaagtacca ctttatccaa tcgttccgat tattgcgatt ctcggtgcta ttttcatttt
    13561 aggtatgacg atgatgacgc aaacgaaatt agcattgatc ggtataggtg ttaccttgat
    13621 aggtatccca gtctattatc aaaaaaagaa aaaacgctca gaagagtaaa atatttcaac
    13681 aacagacaga aaaagtacga actaggtcct ctagatcttc gtgctttttc tatttcaata
    13741 aaacaagttc ttctattttg atttttgcta cttctgcaag tagtgcttat gtgctaaagt
    13801 aagaatgaaa aggagagata aacatgatat ttgattctca tacccattta aatgctgaac
    13861 agttcaatga agatattcca gaaacgattc agcgagcaca agaaatgggg gtaacgaaaa
    13921 tggcggtcgt gggcttcgat acgccaacga ttgaaaagtc tttgcaatta agccatgact
    13981 atccaaatat atacagtatt atcggctggc atccgacaga ggctggcagt tatacgaaag
    14041 aaatcgaaaa taaattacaa gaacaattga ctttaccaaa ggttgtagca ttaggggaaa
    14101 ttggcttgga ttatcattgg atggaagacc cgaaagaagt ccaagataaa gtatttcgca
    14161 gacagattgc tatcgcaaaa gaaatgaacc taccaatcag tattcatact agagaagcac
    14221 ttgaagacac gtatcaaatc ctaaaagaag aagatgttcg agatatcggt ggaatcatgc
    14281 atagtttcag tggtgattat gaatgggcaa aacgatttct tgacttaggg atgcatattt
    14341 catttagcgg tgtcgtcact ttcaaaaaag cattagacgt ccaagaggct gcagcaaatg
    14401 taccaatcga acgattactt gtggaaacag atgcccctta tctggcacca gttcctttta
    14461 gaggaaagcg aaatgaacca ggctatactc gttatacagt agaaaagatt gctgaacttc
    14521 ggaatcttcc gacagaagaa gtggctgccc aaacatggac aaatgcgcac cgattatttg
    14581 gtctaagcga acatggataa gatgaagatc caagagatta tagtcgttga aggaaaagac
    14641 gatacaagaa ggctgcggga agtagttgat gcagatacga tagaaacaat agggtctgca
    14701 atcaatgaag agatcctgat gcaaattgaa catgcccaag agactcgtgg agtgatcgtt
    14761 tttaccgatc ctgatttttc aggagaaaaa ataagaaaaa tcattatgga agctgttcct
    14821 caggcaaaac atgctttttt gccgcgcaca caagctaaac caaagaaaaa gggaagcctt
    14881 ggtgtcgagc atgcaagtga tgctgctatt ttagaagcat tgaaaaaggt cgtgacgcca
    14941 gacatttccc aaacggctgt ttctgaaatc actagacaag accttttaga ctatggttta
    15001 atagctgggg cacaagcaaa agaaaaacga gaaaaactag gtgacgagtt gcgcatcggt
    15061 tacacgaacg gcaaacaatt ggaaaaacgt ttgaagatgt ttcgtattac gccgaaagaa
    15121 ctcgcccaag caatgaaaat agtggaggaa caacatgagt gaatatcgtg aaattgcgac
    15181 accctctaga acaaaagaga ttttattgaa acatggtttt tcatttaaaa aaagtttagg
    15241 acaaaatttt ctgactgaac caaatatttt aaggaaaatc gtagaaactg cgcaaataga
    15301 tgaacatacg aacgtgatcg aagttggacc aggaatcggt gcattgacgg aacaattagc
    15361 caagcatgca aaacaagtcg tagcatttga gatcgatgat cgtttgatcc ctgtattggc
    15421 tgatacgatg caaccctatg acaatgtgac tattgtacat caagatattt taaagaccga
    15481 tttgtcaaca gctgttagag aaacatttca tgaggagctt ccgttaaaag tcgtagcgaa
    15541 tttaccttac tatattacga caccgatcat gatgcatttt cttgaatcag atttggttgt
    15601 ggatgaacta gtcgtcatga tgcaaaaaga agtagctgat cgtatctctg ctgaaccagg
    15661 aacgaaagct tatggttcat tatcgattgc tgttcaatat tatatggaag ccagtttagc
    15721 attcatcgta ccaaaaacag tatttgtccc tcagccaaat gtagattcag cgattttgaa
    15781 actgacaaga agagatatac cggcagtcga agtgacagac gaaaaagcat ttttccgatt
    15841 gacaaaagcg gcatttcagc aaagaagaaa aacgctttgg aataacttgc agcattcgta
    15901 tggcaaagat gaccagacaa aagcatggtt agcaaaaagc ttggaaacag ctggaatcga
    15961 tccaaaacgt cgtggggaga ctttgtcatt acaagaattc gccgcattaa gcaatgcgat
    16021 gtcagaaaat atgcagtaaa aaaagaggtt gtgacagaag ttgtgaaaaa gctgtcctgc
    16081 atcaagtaat aagaacagcc aaacaaaaat agttcctcat gtttttcgtt tgtttgggct
    16141 tattaccgca gatgcaagct tttgaacacc gtttaagaaa gaggttgtgg gcctgccgtt
    16201 taactctaag tcacaaggaa caatttcaaa aaacagcttc tcatgttttt gagttagttg
    16261 gtcttattac ttgatgcaag cttttgacca ctattatgcc gaacaaggag atgtgacaca
    16321 aattttgtca catctcctat ttttgtttct tgatcaaaaa attcttatac ttgatactca
    16381 cactcatttt gtatggattt agaaaagaat atgtgtcttg acagttatta gtaagtgagt
    16441 tatactaagt cgacatttat gtgtcaagac atattaggag agagaaaaaa tgagaaaaaa
    16501 ttcagttcga aaactaacga tttctgcttt attgattggg atgggggtca ttatcccaat
    16561 ggtcatgcct aaaatcatga ttccaccagc ttcctttact ttggcgtctc atgtgccatt
    16621 atttattgcg atgtttttta caccaggagt tgcagtggct gttgcgctag gtacgacatt
    16681 tggtttcttt ttaacaacgc cggttatcat cgcattgcga gcattgtcac atcttgtgtt
    16741 tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt atcgttttaa aaaatggaca
    16801 gtttactttt gctaattggc gtttccaagg gttcaatttt gtgatcggtg tgattcattc
    16861 tttagcagaa atgctagtag tcagcatctt ttactttatt ggtaatatgc ctgaaacata
    16921 ttattctcaa ggatattttt atacagtatt catcttgatg gggattggtg ggcttatcca
    16981 cagtttagtt gattataata ttgcttattt tatagcagga acactcagca aatattttga
    17041 tatccccgta tttacggcag caaaaaaaga aaatcggaaa gagtcgattc aagggtacgc
    17101 gaagaaaacg acatattaga catgatctct caaaaaaaaa actctcatct agtaatggat
    17161 aataaatcct ctaagatgag agttttttgt cctaaaaaga gctgcaccaa catcttctgg
    17221 cacagctcta acagggggca atttatggaa gtttgatgac ttgttctgtt tctttatcaa
    17281 agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt ttatggaaat
    17341 ctcttgcatc tacttttgag atgaactcag tagaaccagt ttttgtgtaa agcatcgttt
    17401 cagctccaag taattcggaa acgacaactt ctgatttcac tacggcttca ggtgaagctt
    17461 caagggctac ttgttcactg tggatgtctt ctggacgaat accgaagata acttcttttc
    17521 cttcataacc tttttcaact aatactttgt tttttccttc aggaatacga agcttaagcc
    17581 catgtccatc tgtgataaca ccatttttca atgtcacatt aaagaagttc atggcaggag
    17641 atccaataaa gccggcaacg aacatattga cgggtgtatc ataaacttct ttcggtgtac
    17701 cgatttgttg gataaatccg tctttcatga taacgattcg gtcagccata gtcattgctt
    17761 ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag acgctggtga agtttggcaa
    17821 tctcagcacg catggctaca cgtaatttag catctaaatt tgataatggc tcatccatta
    17881 agaagacttt tgcatcccga acgattgccc gtcccaatgc cacacgttgg cgttgtccac
    17941 cagataaagc agctggtttt cgtttcaagt attctgtcag acctaagatc tctgctgcat
    18001 tgtccacacg ttttttgatg tctgctttat catatttacg taatttcaag ccaaaagcca
    18061 tattatcaaa aacagtcata tgaggataca atgcgtagtt ttggaaaacc atggcaatat
    18121 cacggtcttt aggtgccacg tcattcatga cttttccgcc gatatttagt tctccttcag
    18181 agatatcttc cagtcccgcg atcattcgca aagtagttga ctttccacaa ccggaaggac
    18241 caacaaaaac gataaattca cggtctttga ttgatagatt aaaatcagtt acagaataat
    18301 tttctgcatt gtcatatttt ttatatacgt ttttcagagc catttctacc ataatacgtt
    18361 ccacactttc ttttttagtt ttattaacta tttaaagtat aaatgaaagc gttaactaac
    18421 ttcaatgtca tgttgaacaa gaattttttc ttttttttgt caatgtgcta gttttaagct
    18481 ttaataaata tccataagaa ataaaaactg atacactgga gtaaataagt atgttaacaa
    18541 tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga aaaaaaccta
    18601 tgatggtgaa attagcaaca gcgtataaaa caatattgga aaaacatgaa ctttttgcta
    18661 ccgggacgac tgggcagagg atcacggaag caaccggctt gccgatccat cggtttaaat
    18721 ccggaccgtt aggcggggat cagcaaattg gagccatgat ttcggaggat cagttagatt
    18781 tggtgatttt tcttcgagac ccgctggctg cacagcccca tgaaccagat gtaacagcac
    18841 ttatccgttt atctgatgtc tatgagattc cgttagctac gaatatcgga acagctgagg
    18901 tgctactaag aggactggaa gccggcttcc tagattttcg aagaatcgtc catgaaatag
    18961 acaagcatcc actttcattt tgatggataa gcgtatggaa aaataacgtt taatgttcgc
    19021 ctatatgtag ctataaaagc taaacaaact gataatatgc gatataatac gaacgtattg
    19081 gataatataa acaataatat tagtttttcg tatttgcagt aaatgtgaaa atatgctata
    19141 gtttctgtgg gaaaagatcc caaaaataat acagagtaga aaataaagcg ttaagtgcta
    19201 agaggatggg atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt attttcgcat
    19261 tcgctgcatg atacgatgta gcagctctta aaggagatgc taaaaatgaa gaaacaccgg
    19321 ttagacgcac gtatgattgc tattatgggc ttattgattg cattgatggt cactttgtcc
    19381 cgactggtag caatcgagac cccatttatt aaaatcagtg tcacatttat tccacaagtg
    19441 atcatgggca ttctttttgg tcctttttgg tcagggatag gcgcagtttt agctgattta
    19501 gtaggaatgg ccctcttctc gaaatctgca ttctttattg gatttacatt gaatgcattt
    19561 attgaaggag caatctacgg attctttttc tatcgaaaag agatcacttg gaaaaatgcc
    19621 attctggcta cgcttagtgt cacgctgatc atcaacttat ttttgacacc attatggctt
    19681 gcgttgatgt accatgtgcc attgttcagc tgggtcgttt gggcgccccg cctgttaaaa
    19741 acagtaatat ggttaccgat tcaatcaatt gccatttatt acgtaggacg tagtattcct
    19801 tacaaaaaaa ttttaagaag tttagcgatc cacgctaaat aaaaaacaag cagtcttccc
    19861 atcccagaag actgcttgtt tttattgtgc tgtgaattgt gcttgataga gccgtcggta
    19921 ataaccatct ttttgaagca gctcatcatg ggtgcccatt tcgacaatag ctccctgatc
    19981 catcactaat atctggtccg catttttgat tgtggacagg cgatgggcaa tcacaaaact
    20041 ggtttttcct tccatcattg ctaagaaggc ttgttggatt tttttctctg tcaatgtatc
    20101 gactgagctg gtagcttcat ctaagatcaa cataggggga ttactgatca tcgttcgagc
    20161 aatcgtcaac agttggcgtt gtccatctga tatctttaac ccttgaccgc caatcttagt
    20221 atctaatttt tccggcaaac gttctacaaa ttcatacata taagaggctt ttagggcatc
    20281 attgatttct gcgtcacttg cttccggatt cccaaaacga agattttctc gcaatgtact
    20341 gtcaaatagc caagtatctt gtaacaccat tccaaaactt ttacgtaatg tatcacgttg
    20401 gatagagcgg atgtcatggt catcgactgt aattgttcct tttgttgtgt cataaaaacg
    20461 catcagcaaa ttaacaagtg tggattttcc ggcccctgtt tttcctacta tagcaatggt
    20521 ttgacctgga taagcagtaa aattgaaatc ttcgatcaaa cgctgcttgg ggtcataaga
    20581 aaaagaaaca tgatcaaaag agacttttcc tttgatattt ttgggttcca aggcataggc
    20641 atctggcaaa tcaggctttt cttctggctg atcgatcaaa tcaaatgctc gttctagacc
    20701 agaaaaagcg gtttgcagtt gtgtcgtgat tccagaaagt tcgataaatg gtttagaaaa
    20761 ctgacttgag tagatcgtaa agctggaaat cacaccaaca gtgattgacg aaccactctt
    20821 caatgccaat aggccgccaa ctaaaccaat cgataaataa gcaaggtgat ctacaaaacg
    20881 agaagacggg tttgtcaagg aagaagaaaa ttgtgcccgt tgtccttttt catatagatc
    20941 agcatttagc agttcaaatt gctgttgact agcttcttct tgctgaaaag ctttcacgat
    21001 tttctggtta ccgattcgtt cggtcacaaa gcccgagata tccccaataa tcctttgctg
    21061 tctatcgaaa tttgtttgtg aagcacgagc aacgatccaa ctcacaagga aaataattgg
    21121 tgtagaacaa agaacgacca atgtcagaag tggacttaga cgcatcataa aaataaacga
    21181 aagaatgatg attgccactc cagaaaataa ttgattgaag gccgcagaaa ttgcaatgga
    21241 aatattgtcc atatcatttg taaatcgact gacgatattt ccgtgggagt tttgatcata
    21301 ataacgaagt ggcaatcgat tcagatgagc aaatgcatct tttcttaatt ttgcaacgga
    21361 taagtaagct aaccgattgc ttaacacttg gatgagccat tgactgacta cgttgattaa
    21421 aacgatacca ccgaatagaa taagtaaatg gaacagtcga ttgaagcgga cctgtccttc
    21481 tccaatcatt tgatcgatac tttgaccaat ttgtaacgtc atcacaactg ttgcaatccc
    21541 attgatgatt cctagtatca cagctagcca aatgtctttt gcataggcag taaggtaagg
    21601 aatgaatcgg cggagcgtct gcaaggataa tttttttact ttcataacta ttcttcctcc
    21661 tgtgatgcga cgatttcctg atattctgct gattgagcaa gtaattcttc atgggtaccc
    21721 tgagcagcaa tcttaccttg atccatcact aagatatgat gtgcttcttg aatagagcgg
    21781 acacgttggg agataataat cactgtactg ccaagataag tctgtagcgc cttacgcaaa
    21841 tccaagtctg tttgataatc caacgcactt aaagagtcat ctaaaatcag taatgctggc
    21901 ttggcaatca aagctcttgc aatcgtcagt cgctgttttt gtccgccaga gaaatttttc
    21961 ccattttcca tgacttttgt atctaaacca tttggaagct gacggacaaa ttcttctgcc
    22021 tgagcgattt tcagagcttc ccaacattct ttttctgtgg cgtctttctt accccattga
    22081 aggttttcac gaatagtccc ggtaaatagt acagaagttt ggggaacaag agcaatttgc
    22141 tgacggagat gatccagctg ccaagaacga acatccattc cgtccattat aactgtaccg
    22201 gaactgacat catagaaacg cggaatcaat tgtgtcaaag tcgttttccc gcttccggtt
    22261 ggaccaatga ttccaaggac tgtatttcgc ttcagctgaa atgtaatatt ttggagagaa
    22321 agaccagaat caggagtata acggaagtca acatggtcaa aagcgataat tgcttcagaa
    22381 gaaaaatcgg gagtagtact gacctcagct tcaagaatcg aattttctgt gtctagcacc
    22441 tctttcaccc gatttcctga agcttcggca cgtgtgaaga tcacgacgag gtttgaaaca
    22501 acgatcagtg ccaaaagcat ttgattcata taattgatca aagcaagaac ttgtccttgt
    22561 tgcaagctac caatatttac tttgatcccg ccaactgtca gcaaacagat gatcccgaca
    22621 ttcatgatca aggttgtagc aggtgataat agggcagaga tattggaaac acgtaaataa
    22681 ttgttcgcta gatcatttgt gcttttatcg aacttctctg tttctttttt tgtctgcgca
    22741 aatgcgcgga tgacacgaac accgctaaga ttctgactaa cgtgttcatt gagctgatct
    22801 agtttttctt gcactttttg gtagagagga acggtttttt tgatgataaa aaataaaatg
    22861 attgagaaaa taggcaataa tgctaagaaa aacagaccta cttcccagtc gatcacaaaa
    22921 gccatgacca ctgacccgat acttaaaaac ggtgctctga tcaataaacg aatgaccatt
    22981 gctaaagcta gctgaagctg attgatgtcg tttgtcattc gggtgatcaa ggtatctgtt
    23041 ccaaaatgat tcaactcgtt gtgagagaaa gtattgattt ttttgattag ctgattacgt
    23101 aactctgtac caaatccttg tgaagctact gaagcatagt attggcaaat gatagcacaa
    23161 atcagaccaa tcacagacat agcgaccatc cataagccca tttcaatgat ttttcctcgg
    23221 tcattcattt ttagtccatt atcgatcaag gaagccatca ttaaaggcag gacaagttcg
    23281 aagacagctt caagaaattt gaagaacggg cccaaaatga tttgcttacg atagtctttt
    23341 gcataacgta ataactgaat catatgattc ctcctattat ttatttgtcc acaattgaaa
    23401 aaacaagtac taccacattg tagcttagga aatttagaat gaaaagagaa caagcctttt
    23461 cataaatgaa agttttatta tgaagaagct ttagaaattg aatagactat tttttttaaa
    23521 aa
//
LOCUS       DAYYAR010000035        23154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000035 DAYYAR010000000
VERSION     DAYYAR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.035"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(160..444)
                     /locus_tag="ACHAT0_001169"
     CDS             complement(160..444)
                     /locus_tag="ACHAT0_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451124.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="ACHAT0_001170"
     CDS             complement(462..1352)
                     /locus_tag="ACHAT0_001170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8451125.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="ACHAT0_001171"
     CDS             complement(1372..1590)
                     /locus_tag="ACHAT0_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8451126.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3240)
                     /locus_tag="ACHAT0_001172"
                     /pseudo
     CDS             complement(1560..3240)
                     /locus_tag="ACHAT0_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            3507..4280
                     /locus_tag="ACHAT0_001173"
     CDS             3507..4280
                     /locus_tag="ACHAT0_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451127.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4361..4930)
                     /gene="thiT"
                     /locus_tag="ACHAT0_001174"
     CDS             complement(4361..4930)
                     /gene="thiT"
                     /locus_tag="ACHAT0_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8451128.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(4996..5098)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5104..6075)
                     /locus_tag="ACHAT0_001175"
     CDS             complement(5104..6075)
                     /locus_tag="ACHAT0_001175"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8451129.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6169..7542)
                     /gene="glmU"
                     /locus_tag="ACHAT0_001176"
     CDS             complement(6169..7542)
                     /gene="glmU"
                     /locus_tag="ACHAT0_001176"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8451130.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8045..8863)
                     /gene="purR"
                     /locus_tag="ACHAT0_001177"
     CDS             complement(8045..8863)
                     /gene="purR"
                     /locus_tag="ACHAT0_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8451131.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9030..9854)
                     /locus_tag="ACHAT0_001178"
     CDS             complement(9030..9854)
                     /locus_tag="ACHAT0_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8451132.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9827..10507)
                     /locus_tag="ACHAT0_001179"
     CDS             complement(9827..10507)
                     /locus_tag="ACHAT0_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8451133.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10530..11468)
                     /locus_tag="ACHAT0_001180"
     CDS             complement(10530..11468)
                     /locus_tag="ACHAT0_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8451134.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11713..12564)
                     /gene="ispE"
                     /locus_tag="ACHAT0_001181"
     CDS             complement(11713..12564)
                     /gene="ispE"
                     /locus_tag="ACHAT0_001181"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8451135.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12718..15291)
                     /gene="mprF"
                     /locus_tag="ACHAT0_001182"
     CDS             complement(12718..15291)
                     /gene="mprF"
                     /locus_tag="ACHAT0_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8451136.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15432..16238
                     /gene="proC"
                     /locus_tag="ACHAT0_001183"
     CDS             15432..16238
                     /gene="proC"
                     /locus_tag="ACHAT0_001183"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8451137.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16317..17873)
                     /gene="rny"
                     /locus_tag="ACHAT0_001184"
     CDS             complement(16317..17873)
                     /gene="rny"
                     /locus_tag="ACHAT0_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8451138.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18284..19333)
                     /gene="recA"
                     /locus_tag="ACHAT0_001185"
     CDS             complement(18284..19333)
                     /gene="recA"
                     /locus_tag="ACHAT0_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8451139.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19442..20683)
                     /locus_tag="ACHAT0_001186"
     CDS             complement(19442..20683)
                     /locus_tag="ACHAT0_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8451140.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20923..21501)
                     /gene="pgsA"
                     /locus_tag="ACHAT0_001187"
     CDS             complement(20923..21501)
                     /gene="pgsA"
                     /locus_tag="ACHAT0_001187"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8451141.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21710..22633)
                     /locus_tag="ACHAT0_001188"
     CDS             complement(21710..22633)
                     /locus_tag="ACHAT0_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8451142.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         6591 a         5039 c         4011 g         7513 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct tttttcatat ctccttgtaa
     2701 aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg ataatctcaa agttgatatc
     2761 atgttccttc agcgcctgcc acacaccaat ctgataagca ccgtgcgctc ctcctccacc
     2821 taaaacaagt gctgtatgat agaccagctt tttatttaat tgtgtggttc cttgatagcc
     2881 gtttttatga aaaatacgca accattcttc tgatagagga gattgaaata aaaaacaaca
     2941 gttttcttta ataaaaaaac gagcgcaagc ttccatatac cgaaaaaatt ttttccattc
     3001 aaaagtttca ccttcaatca acagattatt aaaaataaca tctcctctac ttttagcaat
     3061 agtaaaatag acggtatatt ttttcgatac tgctttaaaa gcacattgct tttttgcaga
     3121 acgccaaaaa gcgtctctac tttcttgatg actttgaaaa aatggaaggg gttgttttgc
     3181 ttgctggata ggcgaataca gcgacatttt ctgtgaaaag taaacttttt ttatctccat
     3241 cgcgctctcc tttttcttca agtataccag tacgtcataa aaaggtcatg agtctcgtat
     3301 tcgactttgt agattccata agaaaacaat tcataaaacc tcctttaaat aaaaaaatac
     3361 aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa tgaaaacgtg attttgtaat
     3421 ggaattttaa aataaaagaa acaagctttt ttaatgcaaa tgttgtaaaa taaaagagat
     3481 taagaggaaa taagaggagg aacatgatga ctgagtcagt ttatgtacat attgatacca
     3541 caagtaatgc tgttttaaca aaagggctga ctgctactga ctttgcaaac agtatcgtgc
     3601 attttcctca aaacttgctc ttgcttgatc catctgcttc tgctggagaa tatgaatccc
     3661 atacagggct gaaagtaatc cgtggtacag aaaatattca gcgtttcttt tcctcttctc
     3721 gtaacaggcg tggattcgat gaattgaagt ggatcgactt tactgattta acgatgctga
     3781 aagaattgac acctttagaa atttcagaat tattatattt tggtcatatg aaaacacatc
     3841 ttcattcgcc gtttttttat aaactacaga ataattttgt atacttcgat cttaatgatc
     3901 agctgaatcg gatttattat cgctatttag atgaatttta taggattttt gctagtaaat
     3961 taagttcgat cttgtcagat aagatcaatg taaaaaaatc ttttttcaaa aaagaaacac
     4021 ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg taatttgatg caggaaggga
     4081 tcattttttc ttttgatcaa gttggtttga tcgatggtaa atatcgtatc ccaatctatg
     4141 tgattgaaga ttatatttgg aaattaaaag gcacccggta tcgagaagag gaaatcatcg
     4201 ctactttaat ctactctgtc accgatcgac actggcaatt ggtagaagat gccgatcttt
     4261 cttatctgcc attgaagtag atctatttct tatcaaaaaa taaaaaggaa acaagaaact
     4321 aggacatgat tctgtctcaa gtttcttgct tcttttttat ttacagctcc tgtcgtttat
     4381 ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa taaaacgatg atcgttacaa
     4441 tagctgtact caacccgctg gcaccattca ttacgaaaga aaagagccaa ggattcatcc
     4501 cccatagtgc atacgctccc cagaaaacca ctcctgcaat aaaatgccaa aaatatctgg
     4561 aaagtgcgcc aatcaatgca ccaaacacga tgctttttct aatttgtttc gtattatcgt
     4621 ctacatagcc tttttggaca tttgaagcca tgagccctgc tgcgcctgca aaagtatagg
     4681 caatcgggta ctcaatcaac acttgaacga cacttaaata ataaacttgt cccaacggaa
     4741 aatgtaggat gccccaaatg aaacctgcca gcatccctgc ctttgtccct cttcttaacg
     4801 caaaaagagt caatggaatc tgccctaatg aaatagaaaa actgctgccg atctggatcg
     4861 gaatcaacga caacaccatc gctaacgctg caacaatcgt tccttctacc caaactctat
     4921 tttttcccat aaaaaaactt cctcctttga ataaaaaagt cacaaaggag gaagcagctt
     4981 tcgaaaatag tcaaacagct gtttattcac aattcctacg ctcgcataac cgatacaggt
     5041 acgaagggtt tagaaataga ttcaatctca gcccaattgg actccccttt gtgagtttat
     5101 ttattattta ttttttgttt caaacaatgg actaactggt ttgttttcat ggatacgttt
     5161 gattgcatct cccattagtc cgccaacact tacttcatcg attttgtcga ttttacgatc
     5221 ttctggaaga tagatcgagt ctgtaacaac tagacgctcg atagctgaat cttctattct
     5281 ttgtaaagca gggccggata atactggatg tgtacatgaa gcatacacac tagttgcacc
     5341 agcttctttc aaagcattgg cagccaatgt gatcgtacct gctgtatcga tcatatcatc
     5401 gatcagtaca caagttttgc ctttgacttg tccaatgata ttcattactt ctgcaacatt
     5461 tgcttttgga cggcgtttgt ctatgatcgc aatcggagat ttcaaaaatt ccgctaattt
     5521 acgtgcccgt gtgacaccac catggtctgg tgaaacaacg acaacatcat cgcctttgat
     5581 tcctttttct aagaaataat tggcaataag tggcgcaccc atcaaatgat cgactggaat
     5641 atcaaagaaa ccttggattt gaactgcatg aaggtccaaa gtcagcattc ttgttgcacc
     5701 agctttttga atcatgtttg ctactaattt cgctgtgatt ggttcccgtg cacgtgcttt
     5761 acggtcttga cgcgcatagc cataataagg catgactacg ttgattgttt ttgcacttgc
     5821 tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag ttatcattga ctgggctgct
     5881 cgttgattga ataacataga catgcgatcc acgaatactt tcttcaatgt ttacttgaat
     5941 ttctccatca ctaaattgag taacagatga ttttcctaac tcgacgccga ctgctgctgc
     6001 aattttctct gctaatgggc gattggaatt taacgcaaaa atttttaatc ttgggtcaaa
     6061 gtaatgtttt gacatgggga cctccacttt ctttttcaca actctagaat tctacctaat
     6121 aaatagtagt catatttttt aaataaatca agtgattttg caaaagtctt agtttagata
     6181 agggagtttt tttgcatatc cttctttatt gacttgtctt gctctcgcaa ttgccagcgc
     6241 atattctgga atattgtctg taattgttga cccggcagca atcgaactgt ttttagctac
     6301 ctcgacaggt ccgatgatat tcgtgctaga accaataaag ctgtggtcgc cgacagtcgt
     6361 atgatgtttg ttctttccat catagttgac gaagacaaca ccacatccga cattgatttc
     6421 ttctcccagc gtagcatcac caacataagt caagtgaccg actttagttc ctttgccgat
     6481 ttgagcattt ttcacttcca caaagtttcc gatatggacg ttttcgccaa tttcagcttt
     6541 tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc ttgacatggc tgctttcaat
     6601 gactgagttt tcaatgacga cgtgatcctc aatcctactg tctacgattt ttgaatgtga
     6661 cccgatgaca caatcagaac caatgactgt atttccttgg atttgtacac ctgcttcgat
     6721 caatgtgtct ggaccgattt ctacgcctgc atcgatgtat gtcgttgctg aatcgacaaa
     6781 gcttacgcca ttgaccatat gcatctggtt aattcgcttt ctcatgatct catttgcttt
     6841 agctaacgcg atccgatcat tgactcccat tgattcttca aaatcatccg tttgataagc
     6901 agcaactgta tggccttctt cttttaagat ttcgatgata tctgttagat agtattcacc
     6961 ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac aaagcttcgt tgtcaaaaca
     7021 gtaagttcct gtattgattt cttgtacaag agcttcttct ggtgttgcat ctttttgttc
     7081 cacgattttt tctacgatcc caatatggtc acggatgatt cgtccatatc ctgttgggtt
     7141 ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt ccttggtgat attcaaaaag
     7201 attgttcagc gtttcagtag tcaataatgg tgtatcgcca ctgataacta acgttgttcc
     7261 tttttttcct tccaagaaag aagcagcttg caaaacagca tgtcctgttc ccaattgttc
     7321 tgcttgtaac gcatatttac tgcgttctcc tagttgtgct ttaacttgtt ctgctccgtg
     7381 tccaacgatc gtaatcactt catctggttt cgtttcactg acccgattaa tgatatgttc
     7441 taccataggt ttaccagaaa cgggatgtaa aactttatat aattttgact tcatgcgggt
     7501 acctttccct gccgccaaaa taattgcata acgtgcgtcc aactgcgtca ctcctaaata
     7561 tagtttgctg atttcagttt atcccagtat acccattttt tcaacaaaac aagtgatttt
     7621 cttttatccc agcgttttct ggataaaaaa atagatagac cataacgggc ctatctattt
     7681 ggtaaccggt gctctcctgc caactcctcg gtcacaagtc acactactca taaaacatga
     7741 aaagctgttt tttgaaatcg tttcttgtga cttagagtta aacggtaggc ttacaacctc
     7801 ttcattaaac gtttttcaat cagctgacct tcggtattag ctcaataaac ggcaaaatat
     7861 gaggagcaat ttttccgttt attgagctaa tactcaggac aggacgctga tttcacaacc
     7921 tctagataaa cggtgttcaa aagccagttt ctcggtatta agccaaattt atgaaaaaat
     7981 gtggaaagcc attttcacaa aatttttctt actactcgaa actaagcagc tttttcacaa
     8041 cctcttattc tgaaaaataa tttcctggca caaccgtaat atttttcgtt tgagtatcta
     8101 catctttgac ataaagcagt gacgtataat catcgatggc gcgatgaccg ttgaattttg
     8161 attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa ttcttcaatc aagctcttca
     8221 ttccgttgac tgttccgccg cctttcatga aatcatccac tacaaggaca cgtgaaccac
     8281 gtttcaaact tctcttagac agttccatct tttcaattct ttcagaagat ccagacacat
     8341 agttgacact tactgttgat ccttctgtta tttttgagtc ccggcgtaca ataacgaatg
     8401 gcgcattcag ataataggac acagcttggg caataggtac gccttttgtg gctacagtca
     8461 ttacagcatc gattttttca ccaaggtatt tagaagcaat gattcttcca acttgacgca
     8521 gcagttcagg ttctcctaac aaatcagaga gataaacata tcctcctgga agcagacggt
     8581 cttgttctga tagacgttct gctagagatt caatatattt tttcgcctct tcatatgaaa
     8641 tttcagggat gaacaataca ccacctgcag cacccgggat tgtttccaag gttccatacc
     8701 ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc actgatagaa gatttagcag
     8761 attcataacg ttcggcaaag cttgttaagg aaatcaattc atgcgggtga ttcaataaat
     8821 actgtgtcat gtcaactaga cgttcactgc gacgtacttt cacctattcc aactcctttt
     8881 aagaaatttt atcgttttac attatataag tttttcttca aaaattcgag cattttatga
     8941 aaaaataaga aaatagttcg gtatttatta aaataaaaag aagcctataa aaaatttttc
     9001 gtttttataa ggcttctttt ctttataatt tagttatttt ttcttctgaa ggcaaccaat
     9061 attcgtttga tgatatttac aatcaggaac aaagcaataa agactaatgt gattgttgct
     9121 cctggaggtg tatctagata aaacgagcta gttagtcctg acagcatccc tagaaatcct
     9181 acacaaacac tgatagcaat cacgacattg aagcttttgc ctaatcgcat accgattgct
     9241 gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg caatcgggat catcacagca
     9301 atagccacac cagtaatcac attgaacatc atcgacatca aatgaacggg tagcccatcc
     9361 acgtgggcgg tatcttcatc aaaagtcaga acatacatcg gccgtttgaa taaaaagaac
     9421 aatataccga tggcaacgaa taagattcct aaaaagatga cttgctccca tgtaatggtc
     9481 acgatcgaac caaacagata ggattggata cttgttgctg aattccctcc gctcaaattc
     9541 atcaatacta acgctagcgc caaaccacca gccattaaaa tggcaatgga gatttctgaa
     9601 taggttcggt agatcgttcg cagatactcc aatatcacag ctgctaatac aacgactagc
     9661 atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta acgctacccc tgctaaagag
     9721 acatgagaaa gcgtatcagc catcagcgat tgtcttctga tcactaaaaa cacacctagc
     9781 atcggggcga ttccggcaat gaagatagcg gcaagaaacg ctcttctcat gaattcatat
     9841 gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg gatctgtctg tccgcatact
     9901 gtttgatatc ttcatgatca tgggtgatca ttagaactga tttcccatgg acatgcgcac
     9961 tatgtcggag aagacgataa aattcatttc tggattcttc atccatacct gttgttggtt
    10021 catcaagaac aaataagtca gggtcagtag caaaaatacg cgctaaactg attcgctgct
    10081 tttgtccgcc agacaattca cctattcgcc gatttcgcat atcccacatc cctacagctt
    10141 ctaaagcacg ctgtacatgt tgatgatctt tttcagagag acgcttgaac cagcggcctc
    10201 ttggatatct tcctgaacgg actaattcga tcaccgtact aggaaaacct gcattgaaag
    10261 atgcaacttg ttgaggaata taaccgatac ttaatttttt tccttcttga tttttctcag
    10321 caatctttat cattcctttt gaaggtttca gtaatcctaa cgatgctttc accaaggttg
    10381 acttggctgc tccattttct ccagttaaaa tgacaaattc acccgcatct acatgataag
    10441 agacatcttc tagtacaggt tcatcatcat agtaaaaggt caaatcagat atttcgatat
    10501 aacgcacctt acaacctcca ttctaaaact taattgatac tttcttttaa agctttcaga
    10561 tttttttcca tgacagaaat ataattttcg cctgctttga tttgttcatt agtcaagctt
    10621 tctaaaggat tcaacacttc tagcttcact cctgtttcat tcgacaatgt ctcagctacc
    10681 ttcgaagagg cattttcttc aaaataaatc actttgatgt tgtttttctt cacaaattct
    10741 tttagctcag ctaagcgact tggtgtgggt tcctgatctg gtgaaacgcc cgaaatcgct
    10801 acttgtttta agtcgtattg attcgctaaa taagcaaaag cagcatgctg agtgacaaat
    10861 gtgcggtttg ttgcatcttt tagctcatcg gagtattttt gatccagtgc ttcaagcttt
    10921 ttgatataag catcggcatt cttcgtaaaa gtttcctttt tatctggata tttttcgcct
    10981 aattcttcag caatcgttct gacttctttt atagctaata ccggatcgag ccaaacatgt
    11041 ggatcgtatt catgtgaatg accatgccct tcttctgatt cttcatgttc gtctgcttct
    11101 tgcgattcta aacggtcgat tcctttagct gcttcgatca tcaaggtctg tttgctatcc
    11161 actgaatcct ttaacgaatc cacaaaaaat tccatgtctg aactattata cacgaaaaca
    11221 tctgcatcgg aaatttctgc tctttctttt gcacttggtt cgaaatcatg cggctccgtt
    11281 ccagcaggga tcaaaagttt cacgtttcct tcatccccta cgatctcttt tgtaaaatca
    11341 tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt ttttgtctgc ttccttgttc
    11401 gtatttccac atgctgcaaa cagtaaagca cctatcatta ccgtagctgt caaaagatat
    11461 tttttcattt atatgctcct ttcaatgaac aaatcggaat tttacgattt attaacgtga
    11521 actaatgtat caaaataaaa aaacacagtc aatagaaatc catcaatttc tgatttaagc
    11581 atgtatattc ttgattaaat cgtaaaatgg atgagttgct gctcatatct agccatttca
    11641 ctacaataaa aaactgaaga ataccatttt aggattcctc agtttttcat cacctatttt
    11701 acttcttata ttttattgca gggttcgtac taaataaact tcctcacaaa aaccttttaa
    11761 cccattatat actctctgcg ctcttgaata atgttgacac aaggcaaaaa cggttggtcc
    11821 gcttccactc atcaatgcag catctgctcc gtacttgatc atacgctctt tgatttgatg
    11881 gatgactgga tgccgctttc ccgttatatc ttctaaactg tttcccattt ttttgatcat
    11941 ctgctgataa tcttgctctc ggatggcttc agcaagtccg tggatgtctt gatgttggat
    12001 ggcttgcgta tctacttctc taaacactgt acgtgtagag acactcattc ttggtttgac
    12061 caacacaacc cagcactgtg gcatagaagg taatacttgg atttcttccc ctttgccacc
    12121 gacaaatgcc gtacttccgt agacacagta aggaacatct gtaccgactt ccaaaccgat
    12181 ttctgccagt tcttctaacg aacagcccag attccataat tggttgatcc ctcttagagc
    12241 agctgcacag tctgtacttc ctccgcctaa tccagctgct actggaatat tcttcttaac
    12301 aaagatcttg acaccggttt tgatattgta tttatttttt agcaataaag cagcttgata
    12361 aacattgttc cgttcatcta ccggtaaaaa tgctttattt gtttctacaa tgattttgtc
    12421 ttctgttatt tcttcaaaaa atagatgatc tgccaagtct acgctcgaca tcaccatttc
    12481 caactcatga tacccatctt gccgtctata taatacatcc agtcctaaat ttatcttggc
    12541 tggcgctttt tcaatcaatt ccatcctctc acccattcga aaagtctaag tcgcaagtat
    12601 tctatcatag ggaaaaataa aagtatagct tatgtctgcc tagtggttga ttttgtcatc
    12661 gtttcaacag ctttcacaga attttctctt cataatccaa acaagttcta gtgattctta
    12721 taattctgtg agcacttctt ctgccaaact cggaacattt cctttttctt ttcctatcag
    12781 actgagtagc tgcatggtag caaatccgaa attacttcct ttcctataag caaaataacg
    12841 tccttcccaa tgatcgacat attttaattt cttttcataa gcagactgga aggaatagaa
    12901 aaaattacca taattatata cgatatgcat gactttttct tgaaagaaag aaaaatctgt
    12961 ttctccaaca ttagctaatg gagctaagcc tagattggct tccgagtagc ccattttctc
    13021 gtatacaata aacagattgg ctaacagata gtcagctaat tcctcagatt ctttaggtaa
    13081 gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc ggctgagcgg tgatgaagcc
    13141 aacgactttt tgatctttac ggacaatacc gacaccgcta gataatacat atgatttatc
    13201 aaatcgtccc acagagaagt atttttctcg tgcaccttga agccattttt gtgaaacttc
    13261 atttacagct tgatgtaatt cttccggcag ttgatcgtac caagtaaaat ggtatccttc
    13321 tttcatcaaa cgttgatatt caagttgctg actcagccga gacaagtctt ctgttgctag
    13381 atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa cccaattcat gaagcatcac
    13441 tacgtagctt tctgatatct tataaaacgc aagctgatag cctaaacaat ctgcttgttt
    13501 cataaactga ttcgttgctg cccgcatcat tttaggattg ccaactggat ctcctaaaac
    13561 aaagcatttg tttcctttga tctgataacc aaaaacgact tgatcttggt cgtcttcttg
    13621 gtaataaaaa taactgtaac ctgacaagcg aagctgatgg cttgtagctg tgcctccgta
    13681 tgtttccaat aaacggtcaa aacggtcttt ttcgtactca ctgccaagct tcgtagtatc
    13741 ttcagctaaa tattgataaa gcgccactaa agctagcaat gagatgccca aaccaataag
    13801 ccctgagaac cacacatcat ctgaaggaaa caaaataaaa tgatttcctg ttattcgacc
    13861 gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa acgataaaca aaaagccgaa
    13921 caagctagca tcaaagatta tagctcccca agaataaacg aattttgttc gataaaattc
    13981 ttttctggat aaccacacag ctaacagaat caagacatat accaatatca actgccaaga
    14041 taacgtacgt gtgattgtat ttaaaatacc aaaagatagt aaagcaatcg taggcagaaa
    14101 tgcctttttg acttttactg aaagtgctct agcaaggccg agtaataaaa agccaaccaa
    14161 tagattcact gtctgatcta aaaaattgaa tgagaacggc aataatactt gaaatagccg
    14221 actgattgca gacagattag tgattgttga tagaagtacc atcatgatcc ccgcaaaata
    14281 gacagcactt acgagcacga tatgcgcaac cttttggaaa aacagtcgcg gaatgttatc
    14341 taaaaatcga ttgattttga ctcctgtatg tgtgatgaac aatagaagtc ccgtcataaa
    14401 aggtagaaca taataaaaca accgataata taatagccaa actactgcta catcttgccg
    14461 aacaggcaat tgactcattc ctaagatcat caaaacgtca aaagtcccca ttccacctgg
    14521 gaccatcgtc agcattccta tcaatgtagc aatcatgaac ataggataga tcgaaagcat
    14581 cgaaatatct attctcatca acttgccgat gatcaaaaat accgcaaggg ctcccgtcca
    14641 ttgtccaaag gatgccagta gcagaccaag caagcctttt ggataaaact cgaagaacaa
    14701 tgtctttctc tttagataag caaaaaagaa aatgactggt gctaaaaaac taccgccgaa
    14761 aagccagatc caatagcttc gaaaaatact ttctgactga ataaaaaaga catcaataaa
    14821 tgtaagaaac gaccatacag acaaaccagt cagcataaat agtgcgactt ttgatacagt
    14881 ggctaaaacc atttttctat ttgtctgctt tccataaaaa ctagcccgca gacttgctcc
    14941 taccacacca ccaaaaccgg cgagattgtt gatcgtgttt gttacccacg cagcaagaag
    15001 aaactctttt ctttccattt ttggttttcc ttgtttttct aagactctga tcgtcaccca
    15061 atcatacaac aacatcggag tgacacccag cagtccagcc agaatcatga gcaggatcgt
    15121 catgcggctt tgttgtccca tcctttgcca aatatcttgc caactcatgc cattagcaat
    15181 atttgcgact tgatttgcca caaagactaa cacggagccg aaaaaaatca gttttaaaat
    15241 cagactatgt tcctttatcc attgcagcat caagtatcgt ttatttttca cattttccca
    15301 ttcctaactt gtatactttt gtgtcatacc ccaatgttat cttatttatt ttaatttttc
    15361 cactttagac ctgttctttg cttgcgatga tagaaaaaga aagatatcct aatgctaaag
    15421 gagaggataa catgaaagta ggatttatcg gcacagggaa tatggcacaa gctattattg
    15481 atggtttatt aaaaaaagga acagtctcga aagaagaggt gttcgtttcc agtggacatt
    15541 ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca tgcatgcaaa agtaatcagg
    15601 aagttgcatc acaatcagat atcgttcttt tagccgtgaa accaaaaatc attgaaaaag
    15661 tattggaaga actaagagaa tatagccaga aagtcttatt catttctatt gcttcaggaa
    15721 aatcattaac agatttagaa acagcgcttg aagatcctca ggcctccatc gtacgtgtga
    15781 tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc gatttgcaaa aatgaagctg
    15841 ccacggagga acagcttcag caagctacga tgatttttga agcaatcggc agcgtctatc
    15901 ctttagcaga atcagatttc agtacattca ttggccttgc tggcagctca cccgcattcg
    15961 tttacatgct gatcgatgcg atgggacgga ctggtgtact gaatggtttg tccaaacaga
    16021 cagccactga gattgctgca aaagcaatta tgggttcttg tcaaaaattc ctttcaagtg
    16081 ataaaaatcc atgggaactt gtagaccaag tttcttctcc tggcggtaca actgttgcag
    16141 gtgtagttgc cttagaagaa gcaggattca tcccagcaat catcaaagga atcaatacga
    16201 cgatcgagaa agatcttgag atgttgaata aaaaataatg atagcaatgt tttttcgtgc
    16261 cttacaaaaa aagaagagct gtggcttttg ccgcggctct tcttttgtat tttttattat
    16321 ttagcataat cagttgcacg agtttcccga ataacgatta ctttgatgtg tccaggataa
    16381 tccagttctt cttcgattcg tttacgaata tcacgtacta aacgaacagc gtctaaatcc
    16441 gagatttctt ctggcttgac cattacccga acttcgcgtc cagcttgaac tgcgtaactt
    16501 gattctacac cggcgaagct gttcgaaata ttttccagac ttcttaaacg acggatgtag
    16561 ttttctaatg attcacttcg cgcaccaggt cttgctgctg agagcgcatc tgctgcagct
    16621 acaaggacag agataacaga agtagcttcc acatctccat gatgagaagc gatcgcgttg
    16681 attactactg ggttttcttt gtacttggca gctaattccg caccgatctc aacgtgagat
    16741 ccttcaatct catggtcaag tgctttccca atatcatgga gcaacccggc acgttttgct
    16801 aattggatat cttcgccaag ctctgctgct aagataccag ctaattttgc aacctcgacc
    16861 gaatgcttca agacgttttg tccataacta gttctgaatc gcagacgtcc gatgatcttg
    16921 attagatctg gatgaagtgt atgagcaccg acttcaaacg ctgcctcttc cccgtattcg
    16981 cgaatacgtt catccatttc tttacgagat ttctcgacca tttcttcgat acgcgctgga
    17041 tggattcgtc catcttgtat cagtttttcc aaggtcatcc gtgcgatttc tcttcggatt
    17101 ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat cgatgattaa atcgatacca
    17161 gtcagcgtct caagcgttcg aatattacga ccttctcgtc caatgatacg acctttcatt
    17221 tcatcattcg gaagggtaac gactgaaaca gtcgtttctg aaacttgatc tgctgcgcag
    17281 cgttgaatcg ctaatgataa taagtttttc gctttgcgat ctgcttcttc tttggcacgc
    17341 tgttcagatt cttttaccat tagagttaac tcatgattca gctcttcttc agtcgatttc
    17401 atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa ttttttctag ttctttttgt
    17461 tgatcttcaa tcattttttc aacgtctttt tctcgctctt caattaattg ctgttttgaa
    17521 ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt tttctaacga gtcatcttta
    17581 cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt gagattttaa ttctaactta
    17641 ctttctctca gctcactttc aacttcagaa cggtatttct gattttcttc cttagcttct
    17701 aacaatgctt ctttttttag agtttctgct tctttttttg cactatcgat gattccagca
    17761 gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg actttgcgac cataaaccct
    17821 aaaccaagac cgacaattaa accgatgata gcgaggagaa tgttgaatac cataaaatcc
    17881 acctccatac tatctttttc atttttatca tctatacagc ttctatgata aattattcta
    17941 aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa acatgcacaa ctttattcta
    18001 atgtttgtac cccgtagtgt caactttaaa taaacaaaga aacggctttc tacttattca
    18061 ttaaacgatg gtttttaaaa taaatgacac tagtttcata taggtaaacc tttaaattga
    18121 gaaacttttc tattaaatta ttctttgtag atgaacagat atttttattt tatgtaccca
    18181 ctcgtataat taaataactc acgatttctt tattcaataa aaaaacggca ggtcaaggag
    18241 agtacctact cttcagacca gccgtttttg tttatcataa tttttattct tctactaaag
    18301 gcaattcttc ttgtcctttt tcatcttctg ggacagcgac ttcttcacca ataccataag
    18361 ctgctctaac tagagcagaa acttctgcca tcatttcagg atgattagcc atatagattt
    18421 tagcgttttc ccgtccttga ccaatccgat cttctttata agaataccaa gcaccgcttt
    18481 tatcaacaat gtctttttct actgccatgt ctagaagttc tccttcttgt gagatcccta
    18541 aaccatacat gacatctact tcagcgattt tgaaaggagg tgctactttg ttttttacga
    18601 ctttgatctt tgtacggtta ccaacaatat ctgtaccttg tttcaattgt tctgcacgac
    18661 gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg tcctccagga gtgatttctg
    18721 gattcccaaa catcacgcct actttttcac ggatttggtt gatgaaaata gcgattgttt
    18781 ttgttttgtt gatcgaacca gagagtttac gcaatgcttg agacatcaaa cgtgcttgta
    18841 acccgacatg tgagtcaccc atttcgccgt cgatttctgc tcgtggaact aaagcagcaa
    18901 ctgaatcgac tactacgata tctacagccc cacttgatac taaagcatca gcgatctcta
    18961 gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc atcgatattc acacctaatt
    19021 tttgtgcata ttgcggatct aacgcatgct cagcatcaat gaaagcggcc gttccgccat
    19081 ttttttgtac ttctgcaata gcgtgtagtg caactgttgt tttacctgaa ctttcaggtc
    19141 catatacttc gatgatacgt ccacgggggt aaccgccgac acctaaagcg acatctaacg
    19201 caagagagcc acttggaatt gtagagattt gttggtcgat tttttcgccc aatttcataa
    19261 tagagccttt accatagctc ttttcaattt ttttcagtgc agcatctaag gctgctttac
    19321 gatcatctgc caatacgata atcctcctta tatttacaaa tcagagcaac atttcattat
    19381 ccttatcata tcttttttag tgaaaaaaag caagaaaaaa ccgaacaaat attcgcattt
    19441 atcattttct taaaagctgt tttctcaaaa gatcaaatcc ttgcataacc gcattatatc
    19501 tgatagcagc acgatctctg gcaaagtgat atttttccga aaccacgccg tctttagatg
    19561 aaatagcgat ccatactgtt cctgcagatt gaccttctaa cgtgtccggt ccagccactc
    19621 cggtgaaagc tacactgtaa tctgttcttg ctatctcacg tgcttgaatg gccatctgct
    19681 ccacacattc tttgctgact gttccatatt ctgctaatag ctccggatca atctccagaa
    19741 aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc tggaaatact tcagatacac
    19801 cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt caaactttcc gctgctgtta
    19861 cagtctgctt gttttctttc agtagttcca ctacgacttt tgctagcgaa ttatcatcgc
    19921 cgtatccata aaaatattcc cctactcgtt cttggatttt ttcttctaat gctaaaagag
    19981 cttgattccc tgcctgaaca tcatttgttt tcactgtcag tcgtaatgtc acttcatttg
    20041 gtttggcata tggtgcgatc gttggattga tctgtgtctc aatcagatct tttagctcag
    20101 tgaccagtcg cgattcacca atcccataaa agcgcaaaac tttagaaatt agtttttctt
    20161 cagaaggaaa acgttcctct agtaaaggac gtacttgttc cacaaacatt ggtttcaact
    20221 cattaggcgg accgggaaga aggatatacg catgtgtatc tgtttgataa aaagtcccta
    20281 aagcaagccc tgtacgattt ggtagaggaa ccccgccttc gataatctgg gactgttgca
    20341 gattgttttt ggtcattttt cgatgggttg tttcaaaata tgccaataat tttttgtatc
    20401 cttccgtatt ttgtatgaga gacttaccga ggtgagccgc agttgcttct tttgtcaggt
    20461 catcttctgt cggtcctaat ccgccacata agagtatgag ttcacttctt gtttcagcta
    20521 gagcaatcaa ctcttctaaa cgctgctgat tatctcctac aactgtttga taataaactt
    20581 cgatacctag atcagctagc tgttctgaca agaaagtggc attcgtattc acgacttgtc
    20641 ctagtaaaag ttctgttccg acagctatta tttctgcttt cattacttcc cgctccctta
    20701 tatagtatac gctgtatttt tatatttttt tatttaaaaa gagggcaagg agctgtgaca
    20761 aaaatcttgt cacagctcct tgttccaaat aaacagtgtt ctcctgccaa aacctcggtc
    20821 ataagtcaca atatacataa aacatgaaaa gctgtttttt gaaattatcc cttgtgactc
    20881 agagttagac ggcaggctca caacctctcc actaaacgat acttacattg atcctttgaa
    20941 cacaccgctg tttttgatga agtaatccac acctgaatag atagtaaaga tcagacaagc
    21001 ataaagcatg atttgatcta ctggaatacc gatcaggctg aatgggatat tattgataaa
    21061 cagcaagata atagcaacca tttgtgtagc cgtcttgact tttcccggcc aagctgcagc
    21121 catgacttct ccatgttcga caagtagtaa acgcaagcct gtgacggcta attcacgaca
    21181 gacaatgatc gctactaccc aaccaggggc ctttccttgg tcaaccaaca tgataaaagc
    21241 agtcataact agcattttat ctgccaacgg gtccgcaaac ttgccaaaat tagtgacaag
    21301 gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg atactagcta aagcaaaaat
    21361 aactgcacct actagctgtg tgacagccag tgtggtgtct cctacgttaa attgtcccca
    21421 atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc atacaaattc taagtacagt
    21481 taatttattt ggtaaattca cactatcgac tcctttcttc cctattctac gtgactatca
    21541 gcgaagtttc caccgatatt atttactttt ccaaatgaat ttattcaaag gagtattttt
    21601 attgaaattg atctttttga attaacgaag aaggaccgcg acacagattt gtcacgatcc
    21661 ttctttttta tacctacaag taaacagatg cgtaaactaa tttatacgat cattctgcgt
    21721 attgaagagt aaggttcaca ttttttggac tctgattgtt ttcccctgga ttaactggta
    21781 cggattctcc attcgctttg atcgttgcat tgcttgctgc accaatcgtg atggtcgcat
    21841 ttgttgctgt ttccggtaac acggtactct gtgtttcatt tgctgccagt gtcccttgat
    21901 aaacataggc attgtctact agcacaccta accatacacg attgacagca gtaaattcaa
    21961 gggttacagg cttggtagcg tctgtgatat ttactgcgat ggcactgcca gaatcttctc
    22021 ctgtagctat tgccattttc ttttcttcac ttgattttgt tgttgattct actgtagaag
    22081 actctttggt ctgcgtgctt tgctctgagg aagaggactc ctcgactgat ccttctactt
    22141 taagcgaaga agctgtgtct ccgattattg gatcagcttg acgatcttgc caagtcatat
    22201 agcccacgac aacgacaatc gccaaagcaa ccaatcctaa taaaatgaca ggaagacttg
    22261 tccaaaagcg acttggatgc gattcttcct gatgcattgc ttttctagaa gtacttactg
    22321 tttcaggctg cggacgttca gggaccgatc ctgtcaattt gcttttccca tcaaaaacat
    22381 cgactaaatg gtctccatct tctcctacag cttcagcgta ttgccgaata aatgcccgca
    22441 cgtaaaattt accaggcaat tggtcaaaat catcattgtc gattgcttcc aaataacgtt
    22501 tttgaatttt tgtaatctgc tgtaattcat ctaaagatat ttttttattc aggcgtgctt
    22561 gccgtaattt ttctccgatg gtttcaaaag ccactcgttc atcttctcca gtctgtttga
    22621 ttctccgaag catctgctgt tgcaacgaaa gacgttatgt ttgggtgctg tgacaaaagt
    22681 catgtcacag ctcctttttt ccgaataaac attgttctcc tgccaactcc tcggtcacaa
    22741 gtcacaatat ccaataaata taagaagctg ttttttgaac ttgttccttg tgactcggag
    22801 ttaaacggca ggctcacgac ctctttctta aacggtgttc aatcagctgt tcctcggtat
    22861 tagctcaaaa accggcaaaa tataaggaga tatttttccg tatgtttctc taatactcag
    22921 gtcaaaacgc tgatttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg
    22981 taataagtcc aaccaagtta aaaacattaa gaagctgttt ttacttggct gttcttatta
    23041 cttgatgcag ggcagctttt tcacaacctc ttggtacacg gtgttccaga agtagcttct
    23101 tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa ttttcgtaaa ttcc
//
LOCUS       DAYYAR010000036        23009 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000036 DAYYAR010000000
VERSION     DAYYAR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.036"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..2606
                     /locus_tag="ACHAT0_001189"
     CDS             135..2606
                     /locus_tag="ACHAT0_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8451143.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="ACHAT0_001190"
     CDS             complement(2709..4445)
                     /locus_tag="ACHAT0_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8451144.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="ACHAT0_001191"
     CDS             complement(4442..5146)
                     /locus_tag="ACHAT0_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8451145.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="ACHAT0_001192"
     CDS             5277..5780
                     /locus_tag="ACHAT0_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8451146.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="ACHAT0_001193"
     CDS             5740..6078
                     /locus_tag="ACHAT0_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8451147.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="ACHAT0_001194"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="ACHAT0_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8451148.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="ACHAT0_001195"
     CDS             7629..8207
                     /locus_tag="ACHAT0_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8451149.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="ACHAT0_001196"
     CDS             8211..8732
                     /locus_tag="ACHAT0_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8451150.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="ACHAT0_001197"
     CDS             complement(8811..9365)
                     /locus_tag="ACHAT0_001197"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8451151.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="ACHAT0_001198"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="ACHAT0_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8451152.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="ACHAT0_001199"
     CDS             9905..10156
                     /locus_tag="ACHAT0_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8451153.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="ACHAT0_001200"
     CDS             10281..11072
                     /locus_tag="ACHAT0_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451154.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="ACHAT0_001201"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="ACHAT0_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8451155.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="ACHAT0_001202"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="ACHAT0_001202"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8451156.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..12997
                     /gene="rplS"
                     /locus_tag="ACHAT0_001203"
     CDS             12650..12997
                     /gene="rplS"
                     /locus_tag="ACHAT0_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8451157.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13370..14032
                     /locus_tag="ACHAT0_001204"
     CDS             13370..14032
                     /locus_tag="ACHAT0_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451158.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14111..17338
                     /gene="fss3"
                     /locus_tag="ACHAT0_001205"
     CDS             14111..17338
                     /gene="fss3"
                     /locus_tag="ACHAT0_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8451159.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17452..17766
                     /locus_tag="ACHAT0_001206"
     CDS             17452..17766
                     /locus_tag="ACHAT0_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8451160.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17779..18153
                     /locus_tag="ACHAT0_001207"
     CDS             17779..18153
                     /locus_tag="ACHAT0_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8451161.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18163..18507
                     /locus_tag="ACHAT0_001208"
     CDS             18163..18507
                     /locus_tag="ACHAT0_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8451162.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18578..19930
                     /locus_tag="ACHAT0_001209"
     CDS             18578..19930
                     /locus_tag="ACHAT0_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8451163.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            19990..21132
                     /locus_tag="ACHAT0_001210"
     CDS             19990..21132
                     /locus_tag="ACHAT0_001210"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM8451164.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21157..21411
                     /locus_tag="ACHAT0_001211"
     CDS             21157..21411
                     /locus_tag="ACHAT0_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451165.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21667..22851
                     /gene="mobT"
                     /locus_tag="ACHAT0_001212"
     CDS             21667..22851
                     /gene="mobT"
                     /locus_tag="ACHAT0_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8451166.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            22848..22985
                     /locus_tag="ACHAT0_001213"
     CDS             22848..22985
                     /locus_tag="ACHAT0_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8451167.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7644 a         4091 c         4955 g         6319 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    12961 cggaaaagct gctcgtatca aagaaatcag aagataaaca cggaaaactt ccttggaaat
    13021 ctgaaatttg tgaaaagtcc ttgtatatca agggcttttt cttttttctc tatcacagtt
    13081 ttaagccatt tttggctgtt ttggaccatt tttcaatcat attcccttaa aaattgtcct
    13141 agattgtccg acattgacta tcagtgtcgt caaaataggg caaaatttag tagtcagctt
    13201 gacgacaaat ataatacgaa aaataccaaa acatttaccg aatttagcaa cttttgaacc
    13261 caagtttctc aaagggtcag ctattagcaa aaaagacaaa acatgtactt ttctactaaa
    13321 aagttgatgt gataggcttc tatctaaata actattagga gtttttatcg tgaagcaaaa
    13381 tgatggacgt atcatttgga aaaatcaaag tgagttaaaa ctgattttaa caattaatga
    13441 attcatcgaa aaacacggaa ttacatctag ccgtcaatac cagaaaaagc tatcagaaaa
    13501 tccaaattct gcaccaagta tgtggttcat taacaaaaaa tatggatcat gggagaattt
    13561 attgattagt ataggacgag aaaatactgg gtatggaaaa tgggctagaa tgtcagaaca
    13621 agaattattg gaaatagtag aagcctttat taagtgcgag aagataacct ctcaacgaat
    13681 gtacgaacaa aaatcagtag ggaagaatat tccttcactt agtaccatta aaaagatgtt
    13741 aggagatata cgtccacttt ttaaagaaaa aaatgatgga tctcgattta ctgatttcga
    13801 actgctgtta gaactaaaaa atgaaataat ccgattaaaa ctacaggatg atttatcaat
    13861 gacaaagttt cgaaaactag ttcaatcccc aaaactacca tcagttgata caattatgaa
    13921 aagaacgaac aaaaattggg aggaattgat ggcagagatt ggatttgatt atcgaagaat
    13981 caaaatttat aagcagagaa acaacctatc caagacaaag aaaactaaat agtatcactc
    14041 taaatcagac gttcaaaatt aaaatttgga cgtctttttt attacccaaa aacgaaagga
    14101 gatttatttt atgaaaaagc caagatttaa gaattggcgt ttgctcgcaa cactgagtct
    14161 attatgccaa acaattggtg gatcgcttgg cccaacgatt gcctttgctg atgaaattac
    14221 tcatccacaa acggtaactg tggaattgga tttggctcac cagtatgctg tcgaaggtac
    14281 atttagcgat ggtcgtccca tgtcagaagt taccgttccg cactatgcag tttacaatgg
    14341 tgtgaagcaa gatattttct gtattgagcc aggggtgccg atttacaatg agttcactcc
    14401 tggttatgag aaaaatccat tgcctgatat gtcggagaaa gcgaaattag tttccgtttt
    14461 atggaaggaa gctggtacgg atgtggatac acatattgtg gcccagaaga tgatttggca
    14521 agaagttaat ggctacacgc ttcactcaat taaacgttct gatggtagtg cagtaaatat
    14581 cgcagccatt gagtcaaaaa tcaatcaagc cattgcggat tatcaaaaga aaccaagctt
    14641 ccataactcc acagtgaaaa ccgtgttagg tcaatcgact actgtgacag atacgaacgg
    14701 cttgaattta tcggagtttg atgaagttgt cgagaatatc gcaaatattg attatcgtgt
    14761 gaacggcaat caactagtga ttactccaaa tgccaattca aaagagagtg gtgaattaac
    14821 cctaaagaag tcagctaata caggaacacc ggtagcttat aaaatggctg gtcaacaaac
    14881 tttgatggcg ggggcaattg ataagcccaa tacctacaca atcaaaattg atgtggaaac
    14941 tgagggttct ttgaagatta aaaaagtcga taaagaaaca ggaaatgttg taccgggaac
    15001 cgttttccat ttagactttg gaaaaacatt acctgcaaaa gacgtgacca ccgataaaga
    15061 aggcattgcg acattggatg agattcctca tggcgcgaaa gtaaccatta ctgaaaaatc
    15121 agtgccagct ccttatacga ttgacactac accaatcacc gcaaccatta aatctggtga
    15181 gactatttct gtgacttcaa aaaatactcg cgaaaaaggt caaatcattt tgaataaaat
    15241 gggggtggaa acaggaactg atctttggaa cgacaaatac tcattagccg gaaatacgtt
    15301 tgccattcgc aaagacagtc caaccggtga aattgtccaa gaaatgacca cggatgaaaa
    15361 aggtcatgcg gaaacaccaa aagagattgc caatgcgttg gaattgggaa cctattatgt
    15421 gaccgaaacc aaagctagta atggctttgt gaataccttt aaaccagtaa aagtcgaatt
    15481 gaactatgtc aatcaaaccg tggctcttgt taccagtaac gtaaaagggc aaaaccaaga
    15541 aatcactggt gaaaccactt tgacaaaaga agacaaagat accggtaatg aaactcaagg
    15601 taaagctgag tttaaaggag ctgaatatac tctctttact gcaaaagatg gtcaagcggt
    15661 caaatggagt gaggctttta aaccagagtt agtgaaggga acgaaagctt ctgatgaaac
    15721 ggtgactttg actttagatg aaaagaatca agttgctgtt aaacacttag cgattaatga
    15781 gtattactgg caagaaacca aagcacctga aggatatacc ttggatgaaa caaagtaccc
    15841 tgtttccatc aaaaaggtgg atgataacga aaaaaatgcc gtgattaccc gagatgttac
    15901 ggcaaaagaa caagtcattc gctttggctt tgatttcttt aaatttgctg gatcagctgc
    15961 tgggactgcc gaaactggct tcaatgatct gacctttaaa gtatcgccat tggaaggaac
    16021 cagtgagatt acaggtgccg aagatgaagc aattacagct tataacgagc aactaggttt
    16081 tgatggctat ggcaagtttg aaaatcttcc ttatggggat tatttacttg aagaagtaga
    16141 ggctccagaa ggatttcaaa aaattacgcc attagaaatc cgttctactt ttaaagaaaa
    16201 taaagcggat tacactaaga gtgaatacat ttttacgatt accgaaaaag gacaaaaaca
    16261 accaattaaa acggtgaccg ttccttacga gaaactgacg aataaagcat tttctgttag
    16321 cttgaatcgt ttgatgctct atgatttacc tgaagaagaa gatagcttaa cttctcttgg
    16381 gacttggaaa gacggaggca aagaattgac agaccttgat tctaccgagc tagttgataa
    16441 attgagctat aacttgcatg aaatcaaaga agactggtat gtggtagcgc aagccattga
    16501 tgtggaagcg acaaaagctg cccaagaaaa agatgaaaaa gccaaaccag tggtaattgc
    16561 cgaaacaacc gcaacgttgg cgaataaaga gaaaacagga acttggaaaa ttttgcataa
    16621 attaaccgca gaacaagttt tggataaaac catcgtctta ttcaactatg tgtatgaaaa
    16681 caagaaaccc tatgaagcag gtgatgatcc agtagcgaaa gatgctagct tgaacaatca
    16741 agcccaaact gtcaattgta cggtggaacg ccatgtttcc atccaaacaa aagcccacct
    16801 agaagatggt tcgcaaacct ttactcatgg ggatgtggtg gatatgttcg atgatgtgtc
    16861 gattacccat gatgtactgg atggctcaaa agaagctttt gaaacaattc tgtatgcatt
    16921 acttccggat aatacgacca aagaaatttg gaaatctggc aaaattgatt atgaagtgaa
    16981 tgacaaagaa ttcactaaaa ccgtacttgc ggaaaaagtg gacacaagta aatatccaga
    17041 aggtacaagt tttaccttca aagaaattaa ctatggcaaa gacggaaaca tcaatgggaa
    17101 acacaatgag gatctaaaag aaaaaactca aactttaaca cctaaaaaag ttccgacaac
    17161 accaagtgca ccagaacaac cggaaatacc aactgttcca agtgactctc aagaatctag
    17221 tcctacagta aagacattcc cacaaacagg ggagaaaaat tctaatgttc tgctgtttat
    17281 tgggtttacc ttgatctttg cgactgctgg gtattatttc tggaatcgcc gaaactaagg
    17341 tgatgtgatg aaacaactaa ataggaaaaa ccaacgtgcg cgaccgcctc ctaaaaaaag
    17401 tggtcgcgct atttttgtat ccaaaactaa aaatgaaaag aggaaaagaa gatggaattg
    17461 aaatttgttg tgccagatat ggccgaaacc tttggaaaga taaggtatgc cggcgaaggc
    17521 gaagttttaa ctgaaggata tgggcggaat actacagtaa ttggtcgtag ttatcatttg
    17581 tattccagta agcaacgagc cgatgatatt gaagtggtgg tagctgcgga agccggcgaa
    17641 aaggattttg accaagatca accactaaaa gccgtgaatc cccatttggt tgccaaaggc
    17701 tatgagattg agaatcgtgg gtttaccgac tacgtgttgt atgtcgatga tttagtgaaa
    17761 gcataggagg aaaagaagat gagattaaca gaaggtattg ttgtagattc aggactaacg
    17821 tttgggaaat tacgattttc cgcattacgc cgagaagtac ggaagcaaaa tgaggatgga
    17881 acggttagta acgaggtaaa agaacggact tataatttga aatcctctgc gcaaggtcga
    17941 atgatccaag ttagtatccc agcaaatgta cccttacgcg agtttgccta tgacgcagag
    18001 gtagagttgg tcaatccaat catggatacg gtcgccaatt atgttttccg agaaggaact
    18061 actgtcaatt ggttcattaa ggccgatgat ttagtcttga aacgacaacc agatcaggga
    18121 aattctacaa accaaaatga aggaaagaaa taggtgaagc gtatggaaat taatgttgaa
    18181 catattttag attgtctcga tcaatatgga aaaggtgagc taacggagga acaactcact
    18241 aaagcattaa cctatgatga aaaaatgttt ctgattatgc atcaagggtt actggaagta
    18301 ggtaacgatg tcgaagaaga ttttgatgta ctgaattggt taggggaaga agagtccttc
    18361 tttatggtta tcgaagtaaa tgaaagtcta tgtcgagaag ccgaaaccgt attggaagaa
    18421 attggtgtgg aaatgccgga tgccattgaa gtttttttaa agcaactagt ggaaacgaaa
    18481 caactcccta tagcagtaaa tgactagcat aactgcttga aattggatca atgaaatcct
    18541 atgaagcttt tttcgattgg aggtggaaac cataaaaatg tacaaagggc atcgtatacg
    18601 ggcaggagat cagcacttgg tttatcactt tgttcttggc tggctactcg ctctgttcat
    18661 cggttggatg agcgtctttt attttcaaga gcttagacaa tttgatattt ctaagctatc
    18721 actttctact atagaaatcg ttcgttctat aaaagattta atctatttgc taggaagtct
    18781 cgttctttca ggaagcacga tgttgcttta tatacacttt tttcaggatc attggcgcag
    18841 tttgtggcat cgacaaaagc tagcccggat gattctagaa aatcattggt acgaagtaaa
    18901 acaaactcag agtgaaggat ttttcaaaga cctcaatagt agtcgaacca aagagacaat
    18961 cagttacttc cccaaaatct attaccgtat gaaggatggt ttactttcta ttcgcgttca
    19021 aatttcactg ggaaaatatc aagatcagct tttaaagctg gaaaagaagt tagaaagtgg
    19081 tctatattgt gaactagtag aaaaggaact caaagactcc tatgtggagt acactttact
    19141 gtatgatata attgccaatc gaattgggat agacgaagta gtggcagaaa gtggggcttt
    19201 gcgattgatg aaaaatcaag tatgggctta tgattcttta cctcatatgt taatcgctgg
    19261 cggcacaggt ggtgggaaga cttacttttt actcactatc atcgaggctt tattaaaatc
    19321 tgatgccgag ctttttgttc ttgatcccaa aaatgcggac ttagccgatt taggtacggt
    19381 aatgcctcat gtctattccc aaaaggagga aatttctgct tgtgtggaag atttctatga
    19441 gcgcatgatg actcgtagca aggcaatgaa agaaatgccc aactacaaaa caggagagaa
    19501 ttatgcgttt cttggacttc caccaaactt tttaatcttt gatgaatacg tggtttatat
    19561 ggaaatgtta acgacgaagg aaagtgcggt gattttgaat aagctcaaac aaatcgtgat
    19621 gttaggtcgt caatctggtt tctttctgat tctagcttgt caaagaccgg atgcgaaata
    19681 tttaggagac gggattcgcg atcaatttaa tttccgggtg gctttgggtc gtatgagtga
    19741 actgggatat tccatgatgt ttggcgaggt tgataaaaaa ttctttatga agcgtatcaa
    19801 aggtcgaggc tatgtagata cgggagggag tgtgattagt gagttttata cgccacttgt
    19861 accaaaagga tatgatttct taagggaaat tagcgacatt ggtggattaa acgttaatac
    19921 ggaaggataa aataatagta tgtagcctag gagtgtcaag aatcctgggc tttttttacg
    19981 aggtgattta tgacattttt agatttattc gctgggattg gtggcttttg cttaggtatg
    20041 gaacaagcgg gtcaccagtg tatcggcttt tgtgaaattg atgattttgc tcgccagagt
    20101 tataaggcaa tccatgatac aagtaaggag gtggaaatgc atgacatcac aagtgtatca
    20161 gacgagttta ttcaatctct cggaccagtg gatatccttt gcggtggatt tccgtgccaa
    20221 gctttttcaa ttgcgggaaa gcggcaagga ttttccgata ctcgaggtac tttattcttt
    20281 gaaatcgctc ggttcgccgc tattctccaa cctaagtttt tattccttga gaacgtccgg
    20341 ggattactca atcacgaagg aggggctacg ttcgagacga tcctccgaac gctggatgga
    20401 ttggggtatg atgtggaatg gcaagtgctt aactcaaagg cctacgttcc ccaaaaccgt
    20461 gagcggatct tccttatcgg acattctcga gacgcatgta ccgaacaagt atttcctatc
    20521 attggatcgt ctccaacatc tgatcaaaac atcagaaact tattaaatat caacccttcc
    20581 aatcgaggaa tggggggaca agtctatggg tcagatgacg tagcgcctac cttgaccggt
    20641 gatgaaggaa tcaaaatcgc attgccggta gatgatggaa tttctgtagc gggtatgtta
    20701 ctgggaaatt ttgaacaagg caatcgggtt tatgagataa ctggaacagc gcctactttg
    20761 tcaacgaaac aaggcggaac gaagatcatg cttcggaaaa acgaaacaga atatcaagag
    20821 gtgaagccag ggaacagtgt gaacctggct tttcctaatt ccacaagtcg tcgaggccgt
    20881 ctgggaaaac aaagtgtgca taccttatta acaggagacc aacaagcagt cgtcacggat
    20941 cagtatcaaa ttcgaaagtt aacacctagg gagtgctggc ggcttcaggg ctttccggac
    21001 tgggcttttg atcgagcttc tcaagtcaat tcagatagtc agttgtataa gcaggctgga
    21061 aattctgtaa cagttccggt catttttgat attgctagaa gattaaagga ggtaaaaaca
    21121 gatgatttat gatgagttga ttggggaatc tactgggtga ttggcaagat tcagtcagat
    21181 ccggaattgg aagaagaact gtaccggctc aattttgata ttcgcaaaaa cggggtgaaa
    21241 gtccctgatg atccgtatgt aattgatgag gaaacggatg ctcgaatgga gctgaatcaa
    21301 gtcatcagtg aattggaacg aattgcggac tttgctaaag aacctgatat ccgccaatac
    21361 ttgtttgaga taaaagcaga gttggaaatt gagggtatta ccgcggagta acggccaaat
    21421 aaaccactga taggcactga ccgcggggga tttgcgaagc agaaatcctc cgtggtgctg
    21481 tgcctgttta tggacggcgt tagccggcca tgatgtctca ccccccgtat ctaacagggg
    21541 ggtacaaaaa cacaaaaaat aagcaagcaa cgataaggaa tggctgtcct atcaaggata
    21601 ataagcaatt tgtgtagatg ttaactgatt tgaaaaagtt aacaccttaa atttggaggt
    21661 gagaacttgg cacaaaggaa tttagattat cggctattaa aagaccggcg taatgaatat
    21721 ggcgtttcac aaaataaatt agctacgact tgtggactta gtcgcccgta cttaaatcag
    21781 atagaaaatg gtggcgtaac cgcatcgaca aaaaccatga ggaaaatttt tgaccaactg
    21841 gaaagtttca atcccgagtt acctttaacg ttactgtttg actatgtaag gattcgcttt
    21901 cccacaacgg atgcgaggaa aatcattcaa gagattctcc atttgaaatt tgattatatg
    21961 ctccacgaag attacgcctt ttactcctat caagaacaat atgttatggg agatattgtg
    22021 gtgatgttgt cacatgaaga agataaaggc gtccttttag aattaaaggg tcgtggttgt
    22081 cggcagtttg aaactttttt actcgcccaa aagcgtagct ggtatgactt tttcgaagat
    22141 tgtctaaaag ccggtggcgt gatgaaacgc ttagacctgg caatcaatga tcgagttggc
    22201 ctgttagata ttcctgattt aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt
    22261 cgtaccttca aaagctatcg ttccggggaa ctgttgaaac ctgatgataa agatggtatg
    22321 ggcaatactt tgtatattgg cagtcttaag agtgaggtct atttctgttt gtacgaaaaa
    22381 gactatgagc aatatatcaa gttagggatt cctttagacc agaccgaaac gaaaaatcgt
    22441 tttgaaatcc gactgaaaaa tgatcgagct tatcatgcaa ttcaagattt gttaaaaggt
    22501 cgcagtattg aaagtaccac attttccatt attaaccggt acttgcgatt tgcggataag
    22561 gtggaaggca aaaggcgaac taactggccc ttgaatgaac aatgggggcg cttcattgga
    22621 cggaatcaga aggaaattca attaacttct gaaccgaagc cttataccat tgaacgaacg
    22681 ttgaactggc ttggacgaca agtcgcccct acgtggaaga tggccaagga gttggatcgg
    22741 ttgaatcaga caacgtacat tcaggatatg gtaaataata ctcgactatc agaccggcac
    22801 aaaaagattt tggagcaaca aagtatggca attgaaaatt tgattatatg aaaagaggaa
    22861 tcgtgatgga aattgtgaaa gatattttat tggtgtttgg tggcagtatg cttggtgtta
    22921 ctgtgatgtg cttgatgcat gcaagtgcag cggcggaccg agcaatggaa aagaaaggaa
    22981 agtgatgaaa tgaatttcgg tcaaaattt
//
LOCUS       DAYYAR010000037        22562 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000037 DAYYAR010000000
VERSION     DAYYAR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.037"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..632)
                     /locus_tag="ACHAT0_001214"
     CDS             complement(51..632)
                     /locus_tag="ACHAT0_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8451168.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(638..1897)
                     /locus_tag="ACHAT0_001215"
     CDS             complement(638..1897)
                     /locus_tag="ACHAT0_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8451169.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1899..2945)
                     /locus_tag="ACHAT0_001216"
     CDS             complement(1899..2945)
                     /locus_tag="ACHAT0_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451170.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3180..3455)
                     /locus_tag="ACHAT0_001217"
     CDS             complement(3180..3455)
                     /locus_tag="ACHAT0_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8451171.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACHAT0_001218"
     CDS             complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACHAT0_001218"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8451172.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACHAT0_001219"
     CDS             complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACHAT0_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8451173.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACHAT0_001220"
     CDS             complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACHAT0_001220"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8451174.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7283..7918)
                     /locus_tag="ACHAT0_001221"
     CDS             complement(7283..7918)
                     /locus_tag="ACHAT0_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8451175.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7984..9387)
                     /locus_tag="ACHAT0_001222"
     CDS             complement(7984..9387)
                     /locus_tag="ACHAT0_001222"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8451176.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(9374..10405)
                     /locus_tag="ACHAT0_001223"
     CDS             complement(9374..10405)
                     /locus_tag="ACHAT0_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451177.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10436..10657
                     /locus_tag="ACHAT0_001224"
     CDS             10436..10657
                     /locus_tag="ACHAT0_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8451178.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10822..11559)
                     /locus_tag="ACHAT0_001225"
     CDS             complement(10822..11559)
                     /locus_tag="ACHAT0_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8451179.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(11712..13052)
                     /locus_tag="ACHAT0_001226"
     CDS             complement(11712..13052)
                     /locus_tag="ACHAT0_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8451180.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(13289..14812)
                     /locus_tag="ACHAT0_001227"
     CDS             complement(13289..14812)
                     /locus_tag="ACHAT0_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8451181.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(15190..15801)
                     /locus_tag="ACHAT0_001228"
     CDS             complement(15190..15801)
                     /locus_tag="ACHAT0_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8451182.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(15924..16049)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(16131..16847)
                     /locus_tag="ACHAT0_001229"
     CDS             complement(16131..16847)
                     /locus_tag="ACHAT0_001229"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8451183.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(16853..17422)
                     /gene="scpB"
                     /locus_tag="ACHAT0_001230"
     CDS             complement(16853..17422)
                     /gene="scpB"
                     /locus_tag="ACHAT0_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8451184.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(17406..18176)
                     /locus_tag="ACHAT0_001231"
     CDS             complement(17406..18176)
                     /locus_tag="ACHAT0_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8451185.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(18173..18544)
                     /locus_tag="ACHAT0_001232"
     CDS             complement(18173..18544)
                     /locus_tag="ACHAT0_001232"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8451186.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(18563..19450)
                     /gene="xerD"
                     /locus_tag="ACHAT0_001233"
     CDS             complement(18563..19450)
                     /gene="xerD"
                     /locus_tag="ACHAT0_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8451187.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(19546..20019)
                     /locus_tag="ACHAT0_001234"
     CDS             complement(19546..20019)
                     /locus_tag="ACHAT0_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8451188.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(20124..20987)
                     /locus_tag="ACHAT0_001235"
     CDS             complement(20124..20987)
                     /locus_tag="ACHAT0_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8451189.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(21183..>22562)
                     /locus_tag="ACHAT0_001236"
     CDS             complement(21183..>22562)
                     /locus_tag="ACHAT0_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8451190.1"
                     /translation="EILENTDKKSTEPDEINSESDAENQSVSKEGTDLEAKIEKEENQ
                     PKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAVLLLGGGSW
                     AYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQDLSKLKNDL
                     SSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLKADKEIQAI
                     ETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQYKTAKEAVE
                     KVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQAAQAAAAAQ
                     AAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAPGVQSKVIA
                     TCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFYIVTINCKT
                     GWFGGNGSN"
BASE COUNT         6236 a         4815 c         3798 g         7713 t
ORIGIN      
        1 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
       61 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
      121 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
      181 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
      241 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
      301 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
      361 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
      421 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
      481 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
      541 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
      601 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
      661 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
      721 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
      781 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
      841 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
      901 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
      961 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     1021 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     1081 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     1141 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     1201 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     1261 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     1321 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     1381 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     1441 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     1501 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     1561 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     1621 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     1681 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     1741 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     1801 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     1861 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     1921 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     1981 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     2041 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     2101 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     2161 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     2221 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     2281 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     2341 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     2401 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     2461 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     2521 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     2581 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     2641 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     2701 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     2761 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     2821 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     2881 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     2941 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     3001 agataagagc aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg
     3061 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     3121 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     3181 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     3241 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     3301 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     3361 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     3421 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     3481 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     3541 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     3601 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     3661 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     3721 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     3781 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     3841 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     3901 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     3961 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     4021 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     4081 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     4141 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     4201 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     4261 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     4321 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     4381 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     4441 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     4501 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     4561 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     4621 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     4681 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     4741 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     4801 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     4861 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     4921 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     4981 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     5041 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     5101 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     5161 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     5221 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     5281 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     5341 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     5401 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     5461 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     5521 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     5581 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     5641 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     5701 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     5761 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     5821 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     5881 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     5941 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     6001 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     6061 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     6121 cgttttgaaa gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact
     6181 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     6241 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     6301 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     6361 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     6421 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     6481 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     6541 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
     6601 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
     6661 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
     6721 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
     6781 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
     6841 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
     6901 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
     6961 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
     7021 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
     7081 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
     7141 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
     7201 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
     7261 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
     7321 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
     7381 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
     7441 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
     7501 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
     7561 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
     7621 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
     7681 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
     7741 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
     7801 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
     7861 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
     7921 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
     7981 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgttcccc aatgaagttg
     8041 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
     8101 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
     8161 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
     8221 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
     8281 catccatttt ttttgcagct catcaaaggg agcttgctct gtggcatatt ccaaataaag
     8341 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
     8401 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
     8461 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
     8521 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
     8581 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
     8641 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
     8701 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
     8761 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
     8821 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
     8881 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
     8941 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
     9001 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
     9061 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
     9121 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
     9181 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
     9241 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
     9301 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
     9361 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
     9421 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
     9481 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
     9541 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
     9601 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
     9661 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
     9721 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
     9781 gttgcagata aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg
     9841 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
     9901 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
     9961 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    10021 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    10081 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    10141 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    10201 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    10261 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    10321 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    10381 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    10441 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    10501 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    10561 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    10621 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    10681 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    10741 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    10801 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    10861 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    10921 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    10981 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    11041 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    11101 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    11161 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    11221 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    11281 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    11341 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    11401 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    11461 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    11521 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    11581 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    11641 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    11701 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    11761 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    11821 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    11881 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    11941 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    12001 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    12061 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    12121 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    12181 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    12241 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    12301 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    12361 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    12421 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    12481 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    12541 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    12601 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    12661 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    12721 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    12781 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    12841 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    12901 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    12961 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    13021 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    13081 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    13141 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    13201 agctattgac ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    13261 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    13321 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    13381 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    13441 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    13501 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    13561 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    13621 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    13681 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    13741 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    13801 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    13861 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    13921 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    13981 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    14041 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    14101 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    14161 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    14221 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    14281 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    14341 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    14401 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    14461 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    14521 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    14581 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    14641 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    14701 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    14761 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    14821 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    14881 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    14941 cctattccct cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc
    15001 ctttttttta taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa
    15061 gaattgattg atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt
    15121 tacattattg catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct
    15181 ttttgatatt tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa
    15241 ccatggaaga agttgcgcat gcagcactaa aaagacagca ctcacgatga atcctttgat
    15301 caagttgaaa ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg
    15361 tgttgcgttc agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat
    15421 actcataaaa atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc
    15481 tttatgctgt ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga
    15541 taagaaacta gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg
    15601 taatactgaa cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct
    15661 gatcaatacc gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg
    15721 aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt
    15781 ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt
    15841 ttgacgtttc tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat
    15901 ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg
    15961 gaatttcacc gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg
    16021 ggaatttcac cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat
    16081 acatatctat tcaaaaaacg caacatctaa cacttataaa aagtaagtgt tcattttgag
    16141 tggttcagca aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg
    16201 ccttgtaaag tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact
    16261 gcttgaagca tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata
    16321 ctgtggtttg tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt
    16381 ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt
    16441 acctttgcaa cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa
    16501 tctccatcat ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga
    16561 tagatacgtt cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct
    16621 gcaacagcag aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga
    16681 taaattggga cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc
    16741 cttacaactt gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga
    16801 cgagaagtga tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc
    16861 ctttattcat tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca
    16921 tttcttgtgt atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc
    16981 cagttgttcc atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca
    17041 gtttatgggc agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga
    17101 tttcagctct tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt
    17161 gagataatgt gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt
    17221 tagtcgttaa tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt
    17281 gagtgtaaga ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta
    17341 acagtccgct taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt
    17401 caagttcact taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat
    17461 tcatagtttt ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc
    17521 ataaaagtag tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag
    17581 ccatgcgcat ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg
    17641 tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc
    17701 tgcatggcac ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca
    17761 tccgctaacc catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct
    17821 ttttctgtca gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc
    17881 aattcttctc gaggatcttc ttcccataat tcttcatcgt ctgtgacgaa ttcttggaca
    17941 ggaagcaatg tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct
    18001 gcaacagcta gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc
    18061 gtagcgatcg gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga
    18121 tcgagaggac cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt
    18181 tcttcttttt ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga
    18241 tggagacatc cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac
    18301 cgcgataaga cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt
    18361 aaataccaat aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat
    18421 aatcgtcttc agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt
    18481 tcttatggaa actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta
    18541 acatcttctc accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca
    18601 ttcttttctt cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca
    18661 attcctgtac tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc
    18721 gcagcgtatg tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat
    18781 ttttccagat tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt
    18841 cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga
    18901 tcgcataatc acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca
    18961 gtccaagaga aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc
    19021 cagtagcata catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat
    19081 caggtgcttc aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg
    19141 ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta
    19201 aaaactgatg gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt
    19261 tttcttcatg caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg
    19321 aagcaatttt ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt
    19381 gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt
    19441 gctctttcat aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga
    19501 tagtcttttt ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt
    19561 tcttttttct ttttcatagc attcctgaca gattccatgg aacgttagcc gatgatcttt
    19621 tactaaaaaa tgataacgtt cttctactac ttgctccact tcaagtaaaa gatcctcttc
    19681 tacttcctcg atcgtgccgc agttcaagca tagtaaatga tgatggaaat gtttggcgcc
    19741 ttcttttctc aaatcataac gggctaatcc atcattaaaa cttactttat cgacaatttt
    19801 caattctgtc aagatttcaa gggttcgata aacagtagca agtccaattt ccggactctt
    19861 ttttttcact aaaaagaaga tttcttctgc agataaatgc tctttctcat tttccaaaag
    19921 cacaagcaga gtagcttctc tttgcggtgt cagcttaaac cccgcttcat gtagctgctg
    19981 ttttgttttt ttcatcgccg aaatagcatt catgaccatt tactcaaccc caacaattta
    20041 taataattac aaataaaaaa agatataaca ctatgttaat taaaattatt ataagctagt
    20101 taagaaaaac atgcaaggga ttctcaatta gattttttga ctagttttgt cacaccgttt
    20161 tcttgtgtaa tcaatccttg tttcaaaaga ttacctaatg cgcgcttaaa tgctcctttg
    20221 ctgataccaa aagctttcat gatttcttct ggatccgatt tatctgtaaa gttgatttgc
    20281 tgatctgcag aacgttctaa aatggttaaa atcatttgtg catcatctga tatagcttca
    20341 tatgctcttg gcttcaatga aagattcagt acaccgtctg gtcggacacc aatcactcga
    20401 ccttttatct gctcgcctag acgaggttca ttatatcgtt cagaaggatg gataaaccca
    20461 agatagaaat cttccgttaa aacgtacgta cctgttaatt tcaagcggta tgctgttcct
    20521 atcacatctt tgtttttcat ttcttctgtt ccatatttgc ttaaagattt gaatattttt
    20581 tcatctgcta aactgcccca aatacggtct tttgcatcaa ctctaaggga aatcatcaag
    20641 cgatcccctt ttttaggcca tagctctgtc attgtcggca attcatcaag agatacgaca
    20701 aagtctttat ctggaagacc gatatcgaca aacgcaccta aatcttttcg tgtagcagta
    20761 acgattccaa aagcgtaatg accgatacgg cttttaggaa tttgtgtagt gatccgattt
    20821 tcatgttttt gattttggta accgaatcct tcaactgctt caccgatttt gtgttcacct
    20881 tctgatttgc ttaagccaaa ggtttgtccg tttttttgga caaagtagtt tttttcattt
    20941 tcatcaataa taatacctgt aaatatagtt gctaataaat cgttcatttt tttctccctc
    21001 tatagtgaaa ggttttctta atacatttct ttgctatcat aacataatgt tatctacttg
    21061 aatagctgag ccttggtttg gaaaggtgaa aaaaggagta tgacaacagt tgtgtcatac
    21121 tccccttttt cgaataaacg gtgttaaacg gcaggctcac agcctctttc atttgcctaa
    21181 aattaattgc tcccatttcc tccgaaccat ccagttttac agttgatggt tacgatgtaa
    21241 aatggcaaag cactttctcc aatgcctttc atcaatgcag attttgttga agtggcatat
    21301 agattataat agccttcccc attttcgatt ctggcttttt ctaatctgta acctccttca
    21361 acgatatatc cacgttggat acatgtagca atcactttgc tttggacacc tggtgcccac
    21421 gtccatgctg gattagatgc atcggcaata tcacttgctc tggcagattt gattggctga
    21481 ttcgctgagt ttgaagagga aggggtcgag gatgccgctt cttgctgtgc tgcttgtgcg
    21541 accgcagctt gttgagcggc ttgagctgca gctgctgctt gagcggcttg ttttgcttgt
    21601 tcttcagctg cttttttcgc ttcttctgct tttttctttt cctcttcttc ttttttctct
    21661 ttatcagtga gcatcttatc tacttgtttc aattgttcat tcaactgagc agatagatct
    21721 gtatttttca ctttttctac tgcttctttt gccgtcttgt attggtcgcg tgttgctgaa
    21781 tccactactt ttccgtcttt aaagactata gctgtttttt ctttagctgt ttgcaattca
    21841 gcatattgat ttttagcttc tgtttgtgct tgttgaatca attttccaaa atctgtgtta
    21901 tccgtttcga ttgcttggat ctctttatct gctttcaact tgatgtcatc tgctagatga
    21961 tcatcgacga tggcaggtgc tatgaaccac tcattcactt tttggatatc tcggatctta
    22021 gattggatat cttggtaagt tgtttctaac tctgaatatc ctttttcatt cttaagactg
    22081 gataaatcat tttttagttt tgataaatct tgatgaatca tatctgcacg aataaattga
    22141 tggtgatcat ctgtataaaa ggcagcaagt ttctcatgaa gttcttccat ttgttgctgt
    22201 ctttttgctt ctgctgctac ttcggcttct actttttgat gatggtcata ataagcccag
    22261 cttcctccac cgagtagaag tactgctgct aaaacgagat aaggtacttt ttttgatttt
    22321 ttcttttgcg ctttatctgg atctgttttt tctaagtttg attcttgatg tgttttttca
    22381 gtatctggca cgattgttat tgatttcgct tctgcttcaa acagcttagg ctggttctct
    22441 tctttttcta tttttgcttc aagatcagta ccctctttgc ttacagattg attttcagca
    22501 tcggattcac tatttatttc atcgggctct gttgactttt tgtctgtgtt ttcgagtatt
    22561 tc
//
LOCUS       DAYYAR010000038        22104 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000038 DAYYAR010000000
VERSION     DAYYAR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.038"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..875
                     /gene="purC"
                     /locus_tag="ACHAT0_001237"
     CDS             147..875
                     /gene="purC"
                     /locus_tag="ACHAT0_001237"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8451191.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            875..1129
                     /gene="purS"
                     /locus_tag="ACHAT0_001238"
     CDS             875..1129
                     /gene="purS"
                     /locus_tag="ACHAT0_001238"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8451192.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1131..1802
                     /gene="purQ"
                     /locus_tag="ACHAT0_001239"
     CDS             1131..1802
                     /gene="purQ"
                     /locus_tag="ACHAT0_001239"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8451193.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1803..4025
                     /gene="purL"
                     /locus_tag="ACHAT0_001240"
     CDS             1803..4025
                     /gene="purL"
                     /locus_tag="ACHAT0_001240"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8451194.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4010..5449
                     /gene="purF"
                     /locus_tag="ACHAT0_001241"
     CDS             4010..5449
                     /gene="purF"
                     /locus_tag="ACHAT0_001241"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8451195.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5472..6524
                     /gene="purM"
                     /locus_tag="ACHAT0_001242"
     CDS             5472..6524
                     /gene="purM"
                     /locus_tag="ACHAT0_001242"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8451196.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6521..7099
                     /gene="purN"
                     /locus_tag="ACHAT0_001243"
     CDS             6521..7099
                     /gene="purN"
                     /locus_tag="ACHAT0_001243"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8451197.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7096..8631
                     /gene="purH"
                     /locus_tag="ACHAT0_001244"
     CDS             7096..8631
                     /gene="purH"
                     /locus_tag="ACHAT0_001244"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8451198.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8647..9921
                     /gene="purD"
                     /locus_tag="ACHAT0_001245"
     CDS             8647..9921
                     /gene="purD"
                     /locus_tag="ACHAT0_001245"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8451199.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10043..10957
                     /locus_tag="ACHAT0_001246"
     CDS             10043..10957
                     /locus_tag="ACHAT0_001246"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8451200.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11090..12655)
                     /locus_tag="ACHAT0_001247"
     CDS             complement(11090..12655)
                     /locus_tag="ACHAT0_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451201.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12648..14099)
                     /locus_tag="ACHAT0_001248"
     CDS             complement(12648..14099)
                     /locus_tag="ACHAT0_001248"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8451202.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14113..15129)
                     /locus_tag="ACHAT0_001249"
     CDS             complement(14113..15129)
                     /locus_tag="ACHAT0_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8451203.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15349..16437
                     /gene="chvE"
                     /locus_tag="ACHAT0_001250"
     CDS             15349..16437
                     /gene="chvE"
                     /locus_tag="ACHAT0_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8451204.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16491..18017
                     /gene="mmsA"
                     /locus_tag="ACHAT0_001251"
     CDS             16491..18017
                     /gene="mmsA"
                     /locus_tag="ACHAT0_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8451205.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18019..19224
                     /gene="mmsB"
                     /locus_tag="ACHAT0_001252"
     CDS             18019..19224
                     /gene="mmsB"
                     /locus_tag="ACHAT0_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8451206.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19481..20458
                     /gene="guaC"
                     /locus_tag="ACHAT0_001253"
     CDS             19481..20458
                     /gene="guaC"
                     /locus_tag="ACHAT0_001253"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8451207.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20722..>22104
                     /locus_tag="ACHAT0_001254"
     CDS             20722..>22104
                     /locus_tag="ACHAT0_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8451208.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLF"
BASE COUNT         7125 a         3730 c         5039 g         6210 t
ORIGIN      
        1 taaaacacga acattaaaag aatctaatgc cttaaatgta catatttttg ttgatttata
       61 aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt atttgctttt
      121 aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat gaaggaaaag
      181 ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat ttagatcaag
      241 caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa ttgaataatc
      301 aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat cattttattg
      361 aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct ctggaagtgg
      421 ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc gaaggtaaag
      481 aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat gatccattta
      541 tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc agtgagctga
      601 aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag gtcggtttgg
      661 ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg cttgcggatg
      721 aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat atggacaaag
      781 acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta tataaccgtc
      841 tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag tatatgtaac
      901 ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag tccatcgtat
      961 gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac aacttgctga
     1021 tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc ttgcaaatgt
     1081 aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac atgaaatttg
     1141 cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca gttcgcgaga
     1201 tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa ggatttgatg
     1261 gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt gcgattgctc
     1321 ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa ccagtattcg
     1381 gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt gtactgagac
     1441 gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac cccaagacgg
     1501 cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct catggggaag
     1561 gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat cagattgcct
     1621 ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt acgaacaaag
     1681 cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac ttgctaggat
     1741 cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag gtgaccgtat
     1801 agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga atctatgccg
     1861 aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta ggaagaatgc
     1921 ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt tcctataaaa
     1981 attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg caaggccctg
     2041 gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc aaagcagaaa
     2101 gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga gttggcggaa
     2161 tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat tcattgaggt
     2221 tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt gcaggtatcg
     2281 gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca tttgatcctt
     2341 gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa cacaaacata
     2401 tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt gcaaaaacag
     2461 gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa gaagaagaac
     2521 agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg cttgaagcgt
     2581 gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat atgggagcag
     2641 caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga cttatattga
     2701 cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg atgttatcgg
     2761 aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa atccaagaac
     2821 tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac gacgggcaat
     2881 atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca ttagctgaag
     2941 atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa tttcagcagt
     3001 tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat ctgctgcagc
     3061 aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa gtacaaacca
     3121 atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg acgaataaag
     3181 cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca aaaatcggag
     3241 gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt aagcccttgg
     3301 cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt tgggaattat
     3361 cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct gttatttcag
     3421 gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca ccaatgattg
     3481 gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc aaaaaatcag
     3541 gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt gaattacaaa
     3601 agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc attgaaaaga
     3661 gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt gctcatgact
     3721 gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat cagctaggga
     3781 tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa tcaagatttg
     3841 tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga gatgctgctg
     3901 ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact gacggtcaga
     3961 tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta tgtcttatga
     4021 agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc cagacgccgc
     4081 tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag gtgcaggtat
     4141 cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc tttctgaggt
     4201 attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg gacatgtacg
     4261 ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat tcaaatttca
     4321 tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt cccttagaaa
     4381 agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga tattgatgca
     4441 tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg ctttgaatga
     4501 agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg ccgctttgga
     4561 cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat acgtgattgc
     4621 ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg tccagcctgg
     4681 tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga aggacacgca
     4741 gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa acatcgcagg
     4801 gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag aatcgccagt
     4861 aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa gcggttatgc
     4921 ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca ttgcccgaac
     4981 atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat tatctgctgt
     5041 acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg ttcggggtac
     5101 gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag tccatgtgag
     5161 gattgcttct cctcctttga aatatccatg tttttacggg attgatattc aaacgcgtaa
     5221 agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg gtgcagattc
     5281 tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa aagatgctcc
     5341 gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt tatatgacta
     5401 tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa agcagaccaa
     5461 gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta gatgtcgaag
     5521 ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa cgtttaggta
     5581 tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt caattaaaag
     5641 agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg gcaatcgaag
     5701 cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat gatatcttag
     5761 cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa aatgatccgt
     5821 tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga aatatggcgc
     5881 tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat tatgatctag
     5941 ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa acgatcaacc
     6001 caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg tactctcttg
     6061 ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg ccagaattga
     6121 aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct tccttgcttc
     6181 cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga gggtttgtag
     6241 aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt ggctcatggc
     6301 cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag atggagatgt
     6361 atgaaatctt caatatgggc atcggtatgg tcctggcagt ccaagaatcg aaagtaaaag
     6421 aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg aaagtgatcg
     6481 ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg ctgtttttgc
     6541 ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa aaggattgga
     6601 atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga aacgtgcaac
     6661 tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa aaaaagagta
     6721 tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg tattagctgg
     6781 atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa tcatcaatat
     6841 ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg cctttgaagc
     6901 tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag atactggacc
     6961 aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt ctttagaaga
     7021 aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa ttattgaaaa
     7081 tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac tgggattgta
     7141 gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg aggaacaaag
     7201 catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac agggtttcct
     7261 gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg tcttttagga
     7321 agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca accaatcgac
     7381 tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga tgtcgaaata
     7441 gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag tgcagcaaaa
     7501 aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt actttcagaa
     7561 ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc aaaagtcttt
     7621 cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg ggtggaagaa
     7681 aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg ttacggtgaa
     7741 aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata ttcgatcgca
     7801 gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga tgcagatgct
     7861 gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa acacatgaat
     7921 ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc gtatgaagca
     7981 gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga tctgccaaca
     8041 gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta tacacaagaa
     8101 gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga ctttgcgcag
     8161 gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct tgtacaagat
     8221 caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg taagccgact
     8281 ccagcagaag caaaagcatt agcgtttgct tggagagctg tcaaacatgt gaaatctaat
     8341 gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca gatgaatcga
     8401 gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg tgcagtttta
     8461 gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa acatggtata
     8521 aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga tatggcgaat
     8581 aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta gagaggagtc
     8641 ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat aagtaaaaaa
     8701 cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc ggggatgaca
     8761 aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt aatccatttt
     8821 gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt gttaaatggg
     8881 atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa agcagcagct
     8941 ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca aatcccgaca
     9001 gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa agaaaatggt
     9061 gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt cgtggctacc
     9121 acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg atttggggat
     9181 agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc ccttttggcg
     9241 tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa gcgggcattt
     9301 gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt accgcaaatt
     9361 tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc tttgggaatg
     9421 aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc tacgaaggaa
     9481 ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca agtcgttctt
     9541 tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa tcgtacagta
     9601 gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc tgccggttac
     9661 cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga gctttctgtt
     9721 tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg acgtctttat
     9781 ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta caccgcgtta
     9841 atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa agctgccaat
     9901 agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca acaaacataa
     9961 tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact tttgttgtgt
    10021 atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg atcagaaagc
    10081 agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag taaaagtgat
    10141 tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt atggaaacgg
    10201 tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac gagaagatct
    10261 ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt ctaaaaaaca
    10321 tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg tggatgcttt
    10381 acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag cgtttgatga
    10441 attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac caatgcaaat
    10501 cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc aatttggctt
    10561 gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg aagcaagtat
    10621 cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga tccaagcatg
    10681 gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa aattccctga
    10741 attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa ctggcttggc
    10801 ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta cgatgaacgt
    10861 agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg aagattggta
    10921 cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag caggtcaaaa
    10981 gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg agatattctg
    11041 tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat tagttatttt
    11101 tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt tttttcttaa
    11161 ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata tctgaaatac
    11221 gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct gtcagataat
    11281 cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaatgagag ggacttaatg
    11341 caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt tctttgataa
    11401 aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt gagggcagag
    11461 cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg tcggcgctga
    11521 ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt ttcttttctt
    11581 tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat cgtgttcgag
    11641 ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg tttttcgata
    11701 atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga agtttcgctt
    11761 cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg gtttcaagta
    11821 tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg acaggaccaa
    11881 tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac gtttgggcta
    11941 cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa aatttgatga
    12001 atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa aacttcaaat
    12061 aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct gctaataata
    12121 cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc aatgtttcac
    12181 tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc aattcgaaaa
    12241 caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt actgcttttt
    12301 ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca tctgcctgta
    12361 cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat atgagacgag
    12421 tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg aaaggcattt
    12481 ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt gccatctcac
    12541 catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca gttatttgtt
    12601 cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta tacatgtttg
    12661 tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt tccttctcgg
    12721 tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag gagacgcttg
    12781 tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt ggctaactct
    12841 ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat aactttgaat
    12901 tggatcatct catcaatttt gcatgtggta atacgaataa atcctttgtg acggacgtac
    12961 ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt taaaatagaa
    13021 acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc gtatcttatt
    13081 tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg aatccagtct
    13141 tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat agtcagagct
    13201 tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt ataaataaaa
    13261 tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg gtattgtttc
    13321 aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat gctagcggta
    13381 agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt ccctgaagaa
    13441 agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct ctctaaaaag
    13501 cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc cactattgtt
    13561 aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc gatctccaca
    13621 cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac ctgattgatg
    13681 atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag ctcgttttga
    13741 taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat ttctttaagt
    13801 gtatttgtat tatttttgat ctctcgaata tcattcctaa gttcttctag gatattttcc
    13861 tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact ttccaaaact
    13921 ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat cgtctgttga
    13981 taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat aacagcaaat
    14041 agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt ttttgtcatg
    14101 atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct gggaaaagac
    14161 agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat ttccaaaata
    14221 acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga ctacacaatt
    14281 gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag ttacaatgat
    14341 caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat
    14401 ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt ctaaatatcg
    14461 tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt gaacaatagt
    14521 catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac gattgattga
    14581 ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac cagcaagttc
    14641 taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc
    14701 ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct cgggatcatt
    14761 cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg cttcttttaa
    14821 tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca ctttataggc
    14881 aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat ttcggtagag
    14941 aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct ttttccgcca
    15001 gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt
    15061 attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa aaaataaaac
    15121 acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca gataaaaggg
    15181 aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga
    15241 caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa tgttaacgct
    15301 ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa
    15361 tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt
    15421 ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat gccgaccaaa
    15481 tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga aaaactgggt
    15541 tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc acaaatagaa
    15601 aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg ttcggcttta
    15661 actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta tgatcgttta
    15721 ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg agtaggtgta
    15781 tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg accattcacg
    15841 attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta caacggagtg
    15901 atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc tggacagacg
    15961 agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc aaggatggat
    16021 aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc gccatatgat
    16081 ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa
    16141 cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc gatcattgcc
    16201 ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa tacgattgaa
    16261 atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga aacttatgat
    16321 aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga taaagataat
    16381 tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa aaattaagag
    16441 gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt atggcggatt
    16501 gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg ttagattgcg
    16561 tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa
    16621 aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat gacggggaaa
    16681 tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa gaaaaaggca
    16741 tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa gagaatattt
    16801 ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa
    16861 catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga
    16921 ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc
    16981 tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg aatttgctga
    17041 gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca cataagctga
    17101 atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag acgattgaga
    17161 cactagaaaa tgaaatgatc agcgaagagc atattattcg cggaatggtg ggcagagatt
    17221 tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt aaagtagaag
    17281 actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac atcaacttgc
    17341 atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc agaactgaat
    17401 tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt atattaaagg
    17461 aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga ctcgcttatg
    17521 tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg gaaaacgtga
    17581 cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa aaagaagtaa
    17641 ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc catcaaattg
    17701 tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac
    17761 aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg gcaaaatatg
    17821 agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt cttatctctt
    17881 ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac gaaggacgta
    17941 ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa ttaatgacga
    18001 aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa agagaaaaaa
    18061 ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt gatcatttta
    18121 gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg tccattgaat
    18181 ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg gatgttatta
    18241 gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca
    18301 attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt tatcgcatgt
    18361 cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta tgcagggata
    18421 ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac acaagttgtt
    18481 ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc tacgggttat
    18541 ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt aggaatgatc
    18601 atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta
    18661 tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc agtcgttgta
    18721 ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt gatcattctt
    18781 ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg tcgacagatt
    18841 tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac aaagcgaatc
    18901 acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt agttttagct
    18961 gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga tgcaatggcg
    19021 gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt
    19081 gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg cgtcggggta
    19141 gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct agacttgtat
    19201 aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag actgtgacaa
    19261 gacatttgtc acagtctttt tttcgctaaa agcgaacatt cattgtttcg tttgtataga
    19321 ctaaaaataa tatatgtttt tttaccattc gtataaattc tctttttgct cttgattttt
    19381 ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag ccttgattaa attaataatg
    19441 ttcgtgtttt gctggatgtg aacatcagga ggataatgta atgaaagtat ttgattacga
    19501 agatattcaa ttaattccta ataaatgtat cgtaaatagc cgttctgaat gtgatacaac
    19561 ggtcacatta ggtaaacata cattcaaaat gccagtagtg cctgccaaca tgcagacgat
    19621 tattgatgaa acaatcgcag aattcttagc ggaaaatggt tatttctata tcatgcatcg
    19681 gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg gattgattac
    19741 ctcgatcagt gttggcgtca agaaagagga atattccttt atagagaagt tggcagaaga
    19801 atcgctaaat cctgattata tcactatcga catcgcgcat ggacatgcta actcagttat
    19861 tgatatgatt cagcatatta aaaaatattt gcctgaaact tttgtgattg ccggtaatgt
    19921 tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa caaaagtcgg
    19981 gattggtcca ggaaaagtat gtattacaaa aatcaagaca ggctttggga ctggaggctg
    20041 gcaactggca gctcttagat ggtgtgcgaa agcagctcgc aagccgatca tcgctgacgg
    20101 tgggatcaga acacatggcg atattgcaaa atctgtccgt tttggtgcga caatggtcat
    20161 gattggttca ttgtttgccg gtcacgagga atctcctggc gaaacaaaag tcgaaaatgg
    20221 aatcgtgtat aaagaatact ttggttctgc ttcagaattc caaaaaggcg aaaaacgaaa
    20281 tgttgaagga aagaaaatct ggatccaaca caaaggatct ttgaaagata cactagtgga
    20341 aatgcaacaa gaccttcaat catctatttc ttatgctgga ggaagagatc tggaagccat
    20401 tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt aatggagaca caatctaaaa
    20461 aataaataaa atattaggat gttgtgaaaa agttgtcctg cattaagtaa taagaacagc
    20521 caaacaaaaa tagttcttta tatgttttgt ttggctggaa ttattatcgc agatacaagc
    20581 ttttgaacat cattattcag aataagggac tgtgataaga tttttgtcac agtcccttat
    20641 gttttgcaaa aaaagttttt cttttttctc gtaaattcgg taaaatcaaa agaaaaaata
    20701 gacaatagaa aagagaagct tatgtgggaa aaagtagaga aaaaacaagg accgatttat
    20761 cgtcaaatta tggaacagat tatgagagaa atcgaaaacg gaaggatcaa tcctggaagt
    20821 caacttgaat cagaacgaaa actagggcaa ctttttggtg tgaatcgttc caccgttgtc
    20881 catgcattgg aagagcttag ggagcttggg gtactaacga gcaaacgtgg cagcggatgg
    20941 tttgttaatc agactgaatg ggggaaattc gctgttccaa gaatcgattg gcggcaaatg
    21001 atctctcctc gttatgagca gacggactgg tatgagcaaa aaataagaga agcaaagcag
    21061 atccaaaaaa catcgtttct tgatttatac gctagcgaga tgccagatga gcttttgccg
    21121 aattttgagt ttccttctta ttctcttgaa gaaatcttag cggaagaaaa agaaatggat
    21181 cttttaggct atactccgtt acgtaacaaa atcaaacggt atttggaaag taaatttgaa
    21241 ttggcatttt cccccaacca gcttcttgtg actggtggcg gtcagcaagc aatctttttg
    21301 attttacaaa cgattctttc agctggagag gggatagctg tagagtcacc ttcttttttt
    21361 taccgattac ctcttttcaa agcttcagga acgcgtttat ttgggatacc gatggatgaa
    21421 gaagggatcg acttagaaaa actgaaagaa tcgatccata aaaataaatt gaaagcagtg
    21481 cttatcaacc cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa acgacgagaa
    21541 aagctagtag aattgtgtcg acagtatcgg cttccaatta ttgaagacga tgtatttacg
    21601 gatctttcat ttctaaacca agaagaagtc cttcctatac gaacgattga tccggataat
    21661 gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat cggttgggtg
    21721 ataggacctg agacttttat ctcgcaacta gctcaagcgc aagaaatgat ggagttttcg
    21781 atgaatatcc ttacacaaat cacagtggct aatgtattta atgaagcaat tgatttgaaa
    21841 atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc ttggggagat
    21901 tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt actatgcctg gattacttgg
    21961 gatgggaaaa aactagaccc cgaactagca gaaaaagtag tagatatggg tcttggaatt
    22021 gcaccaagtc tactttttgg gcaagatata aatggaatca gaatcaatta cagtaggctg
    22081 gatgaaaacc aaataccgtt gttt
//
LOCUS       DAYYAR010000039        21966 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000039 DAYYAR010000000
VERSION     DAYYAR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.039"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..1849
                     /gene="efbA"
                     /locus_tag="ACHAT0_001255"
     CDS             143..1849
                     /gene="efbA"
                     /locus_tag="ACHAT0_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8451209.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2007..2909)
                     /locus_tag="ACHAT0_001256"
     CDS             complement(2007..2909)
                     /locus_tag="ACHAT0_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8451210.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2906..3598)
                     /locus_tag="ACHAT0_001257"
     CDS             complement(2906..3598)
                     /locus_tag="ACHAT0_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8451211.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3641..4282)
                     /locus_tag="ACHAT0_001258"
     CDS             complement(3641..4282)
                     /locus_tag="ACHAT0_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8451212.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4320..4634)
                     /locus_tag="ACHAT0_001259"
     CDS             complement(4320..4634)
                     /locus_tag="ACHAT0_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451213.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4622..5251)
                     /gene="pyrE"
                     /locus_tag="ACHAT0_001260"
     CDS             complement(4622..5251)
                     /gene="pyrE"
                     /locus_tag="ACHAT0_001260"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8451214.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5258..5974)
                     /gene="pyrF"
                     /locus_tag="ACHAT0_001261"
     CDS             complement(5258..5974)
                     /gene="pyrF"
                     /locus_tag="ACHAT0_001261"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8451215.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5971..6903)
                     /locus_tag="ACHAT0_001262"
     CDS             complement(5971..6903)
                     /locus_tag="ACHAT0_001262"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8451216.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6900..7676)
                     /locus_tag="ACHAT0_001263"
     CDS             complement(6900..7676)
                     /locus_tag="ACHAT0_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8451217.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7730..10915)
                     /gene="carB"
                     /locus_tag="ACHAT0_001264"
     CDS             complement(7730..10915)
                     /gene="carB"
                     /locus_tag="ACHAT0_001264"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8451218.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10920..11996)
                     /locus_tag="ACHAT0_001265"
     CDS             complement(10920..11996)
                     /locus_tag="ACHAT0_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8451219.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12001..13281)
                     /locus_tag="ACHAT0_001266"
     CDS             complement(12001..13281)
                     /locus_tag="ACHAT0_001266"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8451220.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13300..14226)
                     /locus_tag="ACHAT0_001267"
     CDS             complement(13300..14226)
                     /locus_tag="ACHAT0_001267"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8451221.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14239..15522)
                     /locus_tag="ACHAT0_001268"
     CDS             complement(14239..15522)
                     /locus_tag="ACHAT0_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8451222.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15536..16075)
                     /gene="pyrR"
                     /locus_tag="ACHAT0_001269"
     CDS             complement(15536..16075)
                     /gene="pyrR"
                     /locus_tag="ACHAT0_001269"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8451223.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16310..17221)
                     /locus_tag="ACHAT0_001270"
     CDS             complement(16310..17221)
                     /locus_tag="ACHAT0_001270"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8451224.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17218..17682)
                     /gene="lspA"
                     /locus_tag="ACHAT0_001271"
     CDS             complement(17218..17682)
                     /gene="lspA"
                     /locus_tag="ACHAT0_001271"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8451225.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17687..18385)
                     /locus_tag="ACHAT0_001272"
     CDS             complement(17687..18385)
                     /locus_tag="ACHAT0_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8451226.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18372..18758)
                     /locus_tag="ACHAT0_001273"
     CDS             complement(18372..18758)
                     /locus_tag="ACHAT0_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451227.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18905..20575)
                     /locus_tag="ACHAT0_001274"
     CDS             complement(18905..20575)
                     /locus_tag="ACHAT0_001274"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8451228.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20758..20991
                     /locus_tag="ACHAT0_001275"
     CDS             20758..20991
                     /locus_tag="ACHAT0_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8451229.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21090..21521)
                     /locus_tag="ACHAT0_001276"
     CDS             complement(21090..21521)
                     /locus_tag="ACHAT0_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8451230.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21600..>21966
                     /locus_tag="ACHAT0_001277"
     CDS             21600..>21966
                     /locus_tag="ACHAT0_001277"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8451231.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNL"
BASE COUNT         6202 a         4912 c         3883 g         6969 t
ORIGIN      
        1 gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt ctagactttt
       61 gctttactat tcggccaaat tggtttttct tctttttttc gttataatga atatacttat
      121 gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg cgatggtcaa
      181 cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc cttatgaaaa
      241 tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat tatctgcgca
      301 cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg ataatccgcc
      361 taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag acatcgaaca
      421 aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt tgggtgatct
      481 acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg tcttagttaa
      541 tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc aaaacactta
      601 tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg tcttgaaccc
      661 atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac tagatccaaa
      721 agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc tgaccaaaag
      781 attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct cagccatcag
      841 ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca caccgattgc
      901 ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac tcagtcaatt
      961 attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac aaggcggaga
     1021 actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga aaaaacgtga
     1081 acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg aactacttac
     1141 tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta attattatga
     1201 agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc aaaatgcaca
     1261 gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg gcgaacaaat
     1321 ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac tggaaattgc
     1381 cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat acctgaaaaa
     1441 gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat acttttcttc
     1501 tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc tgacaatgaa
     1561 gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt cgcacgtcat
     1621 cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt tagctgccta
     1681 tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg taaagcatat
     1741 ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa agacgattat
     1801 cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat caatgagtgt
     1861 ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat tgtttgctac
     1921 aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca acggtgttca
     1981 ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg agtaagctct
     2041 ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat gatataaaat
     2101 tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat ccctccaatc
     2161 acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat caaagtacct
     2221 cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc ttcataaaaa
     2281 tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa caattcttct
     2341 ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat atgcgttttg
     2401 aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct gataccagct
     2461 tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt gacttcttcg
     2521 atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc tttgaattcc
     2581 tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc gatcgaaaaa
     2641 ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg gaccaatatt
     2701 ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc tcgattcgtt
     2761 cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc tgaaagactt
     2821 ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt tgctacagcg
     2881 accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa taaatttctc
     2941 ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca tcgaagggga
     3001 gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg attggcccgc
     3061 tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc tccggaaaga
     3121 acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc ccattttctt
     3181 gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca actagattct
     3241 cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt ccgttgatga
     3301 tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct ttcttgaact
     3361 ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat tccttgtcag
     3421 taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg gtttcttccc
     3481 ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca aaccaatcac
     3541 ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg acatccatat
     3601 tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct tctttcttta
     3661 caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg ttcaatagtt
     3721 ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag cttttaatcg
     3781 tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt ttgatgattt
     3841 catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt tctccttcta
     3901 cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac tcagctggaa
     3961 tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc atccctctta
     4021 actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc tctgtgtagt
     4081 catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca ttcgcttctt
     4141 caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct gcaactgtat
     4201 gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg ttcaatccta
     4261 aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt ttccgaatat
     4321 catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt tgaaacagct
     4381 cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga gattattctc
     4441 ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa ctgatcacga
     4501 tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt ttggattttt
     4561 gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc atcaagcgcg
     4621 cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt aattcttgct
     4681 catcgatata acgatcttct aaagctgctt caatcaatac agaataattg gtcaacgtca
     4741 taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca tacgtaaaga
     4801 tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct gcttccaaga
     4861 cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct tcaaatatac
     4921 gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata tagaccattg
     4981 gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt gcagttccag
     5041 caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct tttgcgatct
     5101 cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt gggcttttga
     5161 ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca atttctaata
     5221 agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct gtttgaatcg
     5281 tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata acgataaaag
     5341 ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct cctgcgactg
     5401 ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc atttctttga
     5461 tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt ttggcacagc
     5521 tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg gcatgcattt
     5581 cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt tttccacctt
     5641 ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata ccgccagcgg
     5701 catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct ttttcgaccg
     5761 tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc tcttttagat
     5821 aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc acaaacaatt
     5881 tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt gaaaaatcta
     5941 atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc tcacttcttt
     6001 gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga gctctttaat
     6061 tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta cagcacttgc
     6121 tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca tcccaatgat
     6181 tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag gcttgattgc
     6241 tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct taaggtcaat
     6301 ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag cttcgattgc
     6361 ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga caggaacaga
     6421 agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg ttccaaaagc
     6481 aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt tcacatttgg
     6541 tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac cagcaacatt
     6601 tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat gcataacaac
     6661 gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg tttccgccac
     6721 tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca tgatcgatcc
     6781 aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac agccgcttgc
     6841 cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag gattctcatt
     6901 catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt cacacttttt
     6961 gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc tatcccacat
     7021 gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag ctgttccact
     7081 gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt cttcgttcct
     7141 aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt agcgattctt
     7201 gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt ggcaaaacct
     7261 aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc atatagagga
     7321 gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag attggacaaa
     7381 tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga aagagtagag
     7441 aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt tgtttcgtgg
     7501 tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg cacgagaata
     7561 tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa tgtcatttca
     7621 aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg tttcatctgt
     7681 cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt taaatagggt
     7741 tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg tctaaagaag
     7801 taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg tctttagaag
     7861 catttccccg atctttatca atcgtattga caaccacttg tgttttacca tctcttatta
     7921 agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt tcaacgacca
     7981 gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta tagccaattt
     8041 ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca atcgtaaata
     8101 atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg tacaaagctt
     8161 tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca ggccctaagt
     8221 aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca tgaacttctt
     8281 ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct cccaaaatag
     8341 ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat ggaacagtcc
     8401 gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg acaaactgga
     8461 tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa tcttcgatcg
     8521 ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa tcccctgaat
     8581 ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt tcaccgtcac
     8641 aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt actggatgtt
     8701 ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg ttttcaacga
     8761 tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga taaccaattc
     8821 tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa ggttgaggaa
     8881 tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga tccaagtctt
     8941 cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct aagttgattg
     9001 ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg atcacattca
     9061 tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa aagtcagtcg
     9121 aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc tggattgctt
     9181 tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg ataggaccag
     9241 atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca tattcatagg
     9301 aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca accatcttat
     9361 atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg tttatttccc
     9421 acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc aatatcttgt
     9481 catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac agtttatcta
     9541 aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac cctcttctaa
     9601 tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt tggattaggt
     9661 catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg atttctaaag
     9721 aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc acttcaccag
     9781 ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca aacggccagc
     9841 gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa gtcgttcctg
     9901 taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca gctagcttag
     9961 cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca cgcggattga
    10021 cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca ttacagcctc
    10081 cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt tgatattcat
    10141 ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg atacccaccg
    10201 ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct ctcatcactt
    10261 catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg gtagcggggg
    10321 aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca cacatacctc
    10381 caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg attttatttg
    10441 caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt ggctgattca
    10501 attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc gcagaaagct
    10561 ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc agctccattg
    10621 ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga cgctcttttc
    10681 gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga tctgccactt
    10741 ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca tatccttctt
    10801 cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct tgaccaatga
    10861 tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc ggcatctgtt
    10921 tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg tagtgcatca
    10981 tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata atctctgtgt
    11041 ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag tttttctgga
    11101 tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc aattcttccg
    11161 gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat tttataggta
    11221 tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa aatcggtact
    11281 tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg atcaccaggt
    11341 ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc atctgtgtta
    11401 tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat actatgtttc
    11461 aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata aggtttgtta
    11521 gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg atcaaatgca
    11581 tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac ttcacggatt
    11641 ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt ttgtttcaaa
    11701 aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg ttcttttact
    11761 actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac cccataatta
    11821 cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc agtgatagtt
    11881 tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact agcatctgca
    11941 ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg ttccatactt
    12001 ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg tttcgccttt
    12061 tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt ctttatcgat
    12121 cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa ccgttagcgt
    12181 tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga tcacttgttc
    12241 caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg ctgtttcgct
    12301 tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta atccatgggg
    12361 ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga tcaaagcttc
    12421 tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat cttcgggaat
    12481 atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt ggattcctgc
    12541 tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc aaacatggta
    12601 atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag actcggttac
    12661 gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca taacgccgcc
    12721 aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt ttgcagcttc
    12781 tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat ttgtgaaagc
    12841 aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc gcagattttc
    12901 tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga tgtcatatac
    12961 ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg cgcatacagt
    13021 agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct tgtatgtaaa
    13081 tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca attgtccctt
    13141 tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg ctttgattcg
    13201 cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat tgatctgacc
    13261 gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc catttagcac
    13321 tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa cgatccgcga
    13381 ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg gtgctgggtg
    13441 cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc catgttcctg
    13501 atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt gcacccgaag
    13561 catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat gtccgtacac
    13621 ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg cgcctagacg
    13681 ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac ctacgatggc
    13741 aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat caagcaagca
    13801 ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact ggattgtttt
    13861 actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg ctacgtctac
    13921 gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt ggacagagct
    13981 tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta catcgaagct
    14041 tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact ggcgcttttg
    14101 cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc ccataacttc
    14161 ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt ctgatttgat
    14221 gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta agataagatt
    14281 taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg tgcctacttc
    14341 aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca gatttttctt
    14401 tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg caattactcc
    14461 aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa tcaaagcgct
    14521 taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga tcacaaacac
    14581 gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg ttacaggagg
    14641 acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg tcttatgaag
    14701 acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa gatggccgat
    14761 atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc caagatatag
    14821 ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc aggcagcgtc
    14881 tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc cgctaataat
    14941 acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga agaaaattgt
    15001 taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc cctgattata
    15061 catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga tgactggacc
    15121 gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt tgatgatcag
    15181 cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg ctggataacc
    15241 atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc ctaagtacgc
    15301 aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg aactgactag
    15361 cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc cgaacatcgt
    15421 gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat cgtggatatc
    15481 taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct ctcctctaat
    15541 cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc tctactaaaa
    15601 tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg attggcaatt
    15661 ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg ccaaaatcca
    15721 ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca tcaatcaaaa
    15781 taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc ggttcttctg
    15841 gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca ggaatatcga
    15901 tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt cctcgtgttt
    15961 tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc tcataagtaa
    16021 ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct tgcattttca
    16081 ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag gaatgtttag
    16141 actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc tacttatggc
    16201 gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac ttaaaggaat
    16261 tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat taaaatgcaa
    16321 tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt gcttcaaaga
    16381 tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg agaaactgac
    16441 cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac ggataaccga
    16501 tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt aattcaacca
    16561 gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac ttcccatctt
    16621 ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca ttgatggttc
    16681 ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa gaggtcttat
    16741 cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc attagcagtc
    16801 cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg ttgatcgtag
    16861 aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta tgacctgctg
    16921 aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca tcttcgtaaa
    16981 cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg gggatcaaga
    17041 cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt ccattgacag
    17101 aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg gtgaataaat
    17161 tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta agttctgtca
    17221 tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa tcactccaca
    17281 tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg tctggaacat
    17341 atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac cagctagaat
    17401 caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa tcatcaatgt
    17461 gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct tcccttctag
    17521 catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac ctggtataag
    17581 atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca actgatccac
    17641 tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa tccttttcta
    17701 tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg ggattcccat
    17761 ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct tcaacctcgt
    17821 aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt tggatgatca
    17881 aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct tttgctagcc
    17941 aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg tataacggaa
    18001 attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga agatcttgtt
    18061 caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga atcagttctt
    18121 gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg acaaaatcga
    18181 ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa agatcatctt
    18241 cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt tcggtaaatc
    18301 ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga ttgaaactga
    18361 ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc ccggtttttt
    18421 atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa taaattttgt
    18481 aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg aagaatcaag
    18541 taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac ggggcttttt
    18601 agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt tatcttttgt
    18661 tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga gctacccata
    18721 ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta ttatgcttta
    18781 gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa attaattccc
    18841 tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta gattataatc
    18901 aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc aatgcagcag
    18961 gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact aaaaatcctg
    19021 cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt gcacctagca
    19081 gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc agtttgtccc
    19141 atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa acaacaccat
    19201 ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt tcttctaggc
    19261 ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca gcttttgcta
    19321 aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca agaatcccct
    19381 gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt acgaattcat
    19441 tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg cgctttaaat
    19501 ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct tttttcattc
    19561 cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc ggcgcttttt
    19621 tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca ccaaatcctg
    19681 cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa atactattgc
    19741 agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt ccgtaaattg
    19801 tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg ccttctgctt
    19861 tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg atccttgcaa
    19921 gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg atttcgctgg
    19981 caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca cccaagccaa
    20041 caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa atcactcggc
    20101 gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga gcagataatg
    20161 cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg atatcttcca
    20221 taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc atcacaggac
    20281 caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg agcgcgtctc
    20341 ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg atggaagtaa
    20401 ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa aaattgattt
    20461 tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat ttactagcca
    20521 cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc ttcatttgat
    20581 tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata tgccaattat
    20641 accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat ttgacgtact
    20701 tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt gagtgctttg
    20761 accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat cagttatgat
    20821 gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt caaaactttg
    20881 gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac acaagctgcc
    20941 catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata gataaacaaa
    21001 tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt cctgtaaaaa
    21061 ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt ataaggataa
    21121 ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac tacttgatct
    21181 ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat ttgcacccca
    21241 gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc tttccaaaaa
    21301 gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg caaacctaga
    21361 aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg acctgtgttt
    21421 tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca atagatgatc
    21481 gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc tctcaccgat
    21541 tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa tctcttttta
    21601 tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt ggaggtggaa
    21661 tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct atagggacaa
    21721 accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag aaaaacgact
    21781 tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct acggtcgaca
    21841 aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa gaacttctat
    21901 ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa ggagcagata
    21961 atcttg
//
LOCUS       DAYYAR010000040        21805 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000040 DAYYAR010000000
VERSION     DAYYAR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.040"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..1065
                     /locus_tag="ACHAT0_001278"
     CDS             100..1065
                     /locus_tag="ACHAT0_001278"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8451232.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1164..2336)
                     /locus_tag="ACHAT0_001279"
     CDS             complement(1164..2336)
                     /locus_tag="ACHAT0_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8451233.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2323..3468)
                     /locus_tag="ACHAT0_001280"
     CDS             complement(2323..3468)
                     /locus_tag="ACHAT0_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8451234.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3482..4406)
                     /locus_tag="ACHAT0_001281"
                     /pseudo
     CDS             complement(3482..4406)
                     /locus_tag="ACHAT0_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4523..5602
                     /locus_tag="ACHAT0_001282"
     CDS             4523..5602
                     /locus_tag="ACHAT0_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8451235.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5599..6227
                     /locus_tag="ACHAT0_001283"
                     /pseudo
     CDS             5599..6227
                     /locus_tag="ACHAT0_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6337..6564)
                     /locus_tag="ACHAT0_001284"
     CDS             complement(6337..6564)
                     /locus_tag="ACHAT0_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8451236.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6667..9255)
                     /locus_tag="ACHAT0_001285"
     CDS             complement(6667..9255)
                     /locus_tag="ACHAT0_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8451237.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9275..9934)
                     /locus_tag="ACHAT0_001286"
     CDS             complement(9275..9934)
                     /locus_tag="ACHAT0_001286"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8451238.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(9983..10486)
                     /gene="trmL"
                     /locus_tag="ACHAT0_001287"
     CDS             complement(9983..10486)
                     /gene="trmL"
                     /locus_tag="ACHAT0_001287"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8451239.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10707..11573)
                     /locus_tag="ACHAT0_001288"
     CDS             complement(10707..11573)
                     /locus_tag="ACHAT0_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8451240.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11682..12323
                     /locus_tag="ACHAT0_001289"
     CDS             11682..12323
                     /locus_tag="ACHAT0_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8451241.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12385..13746)
                     /gene="mgtE"
                     /locus_tag="ACHAT0_001290"
     CDS             complement(12385..13746)
                     /gene="mgtE"
                     /locus_tag="ACHAT0_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8451242.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13764..14663)
                     /locus_tag="ACHAT0_001291"
     CDS             complement(13764..14663)
                     /locus_tag="ACHAT0_001291"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8451243.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14665..15462)
                     /locus_tag="ACHAT0_001292"
     CDS             complement(14665..15462)
                     /locus_tag="ACHAT0_001292"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8451244.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15440..16144)
                     /locus_tag="ACHAT0_001293"
     CDS             complement(15440..16144)
                     /locus_tag="ACHAT0_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8451245.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16275..16850
                     /locus_tag="ACHAT0_001294"
     CDS             16275..16850
                     /locus_tag="ACHAT0_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8451246.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            16968..17645
                     /locus_tag="ACHAT0_001295"
     CDS             16968..17645
                     /locus_tag="ACHAT0_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8451247.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(17724..19532)
                     /gene="pepF"
                     /locus_tag="ACHAT0_001296"
     CDS             complement(17724..19532)
                     /gene="pepF"
                     /locus_tag="ACHAT0_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8451248.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(19708..20706)
                     /locus_tag="ACHAT0_001297"
     CDS             complement(19708..20706)
                     /locus_tag="ACHAT0_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8451249.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(20932..21585)
                     /locus_tag="ACHAT0_001298"
     CDS             complement(20932..21585)
                     /locus_tag="ACHAT0_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8451250.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         6431 a         4669 c         3795 g         6910 t
ORIGIN      
        1 ttctttcatt caaaaaatag aaaaagcttg ctaattattg gttaaccagc aaacaggagt
       61 attaaaatat cttgttttat tgaatagcag gaggaaatca tgaaattcca ttatcatata
      121 ttgatcaatc catcggcagg cagcggaaat ggctataaag tagcagaaag aattttaccg
      181 gtattaaaaa ataagcatat tgactataca cttcattata gtgaatacaa aggacacgat
      241 gctgagattg ctgaaacttt ggcaaaagaa acattgattc cttggataga agaagaacag
      301 aaaacaatcg atactttccc ccttttgatc atcgtaggag gtgacggcac cttacatcag
      361 gtacttgata ctttctatca gatggaagta gaattcccag ttgcttatat tccagcaggt
      421 tctggagatg attttgctcg aggggcaggc cttccaaaaa atcccaaaaa aggacttcag
      481 ctgattttag cagctcaatc accagaaaaa gtccacataa tggcttatga agaaaaaata
      541 agtgaaaaaa aaggaatagc tgttaataat ttcggtatag gattagatgc agctatcgtc
      601 catgcgacaa atcattcttc cacaaaaaag cgattgaaca aatacaactt aggatcattt
      661 tcgtatttat tctctattct ccgtgcttta ttcactcaaa aaggatttcc aattctggta
      721 gatgcaggag gaaaacaatt aagtttttca aacgcatttt tatgtacagc aacgaagcat
      781 cctttttttg gcggaggaat cgcgattgtt ccaactgctg atgcgtctaa accagtcatt
      841 gattttgtcg tggtcgaacg aatcaatttc tttaaaatct tgtggctcat tcttttactt
      901 atacagaaaa aacaattgaa atcgaaatat ttccatcact acacaacaaa cagattacgt
      961 atcgtttcaa caacaccgca acatggtcaa gaagatggag aagaaatgga aaaacgacct
     1021 tttgatatca caataaccac aactaaacag ttgttttggt gttaataaaa gaggttttgt
     1081 cagaagctac atctggaaca ccgtttattc ggaaaaaagg actgtgacac gacttttgtc
     1141 acagtccttt ttttactatg cagttaattc tttgcccaat tgggttgcca tcgatagttt
     1201 ctttctttac ccaaaataaa ttggatcatt gaaaacaccg cagcaaaagc aactaagaca
     1261 caaccattga accaaaaaga ttctgcaaat gtttgattgt aactgagtgt ttcactgatc
     1321 aagttattat tttgaacaaa ccaatatgtt ggtgtgaatg ccgctatttt gctaaccatt
     1381 tctggcaagt attctgctgg tacaaacacc ccaccgataa agcaactgcc tgtgataaaa
     1441 atatctttga tcccaccgct ggtttctgag ttttttacca atgaagtgat acaagccgaa
     1501 aagctaatga tcggcaataa aaacaagaaa gtatttaaaa tatgatacca taccgttata
     1561 tcccacgagg tgtggatcac taacagaacc attagaagta agaagccact gaacaacagc
     1621 agcgagtaac taatcaaact aaaggttact ttgcgattca agtttcgttt ggtaatgggc
     1681 gcactctggt ttcgtttact gatctctttg cgattaaatg ctagattgat gacgctgaaa
     1741 ccgctaaaaa tggatagaaa caacccataa gacaagaaat taaaggttgt gcccgtcaac
     1801 agctttttca tccgctgact atatcccgca cttagctcaa cttctccagt aaccgagagt
     1861 gccatttgtg tctgttccat agctgcatcg attgttttat cttcttcttg aaacagaatc
     1921 agtgtttgca aaaaggtgtt cacttgctgt gtaacaactg ttttagaaaa ggcatctggg
     1981 cgactctgta tcttgatcgt cggcatcttt cctacttcga cttgttcggt aaagtctgca
     2041 ggcagatgta agataatgtt tacacgatta aaataaagag catcatcgat ttctttttgt
     2101 gaggttccat ccaatgtaac gactgtttcc ttgtcattca aatatttacg aaggccatcg
     2161 actacaggcg actcttcttc actaatcaag gcgattttcg catcttgcag agaggtattt
     2221 tcaacatcaa tattttgcgc aaaaaagaat gtggcaacaa cagtgataat tacacctatc
     2281 agtaaattcg atttatttgc tttaagcaat gtccaaatcg ttttataaag cgtcataacg
     2341 cacccctctt tccatccaag catttcctaa cgcaaaaagg aaaataaatc cgatcaacca
     2401 tagaagattg gtataaaaac tagccaaatc agtgtagaaa taaagtttat acaaactttc
     2461 gctgatcaag ttgacgagat ttaattgacc aacaatcgga atatgaagat caatccaata
     2521 cttcacttgt tccgacccca tcataccagc aaaaaagctc atcgcaatcg tcactgaaat
     2581 caagatgcta tccttttgca ccagattcat ttttggtatc aaattaccaa ccaatgtacc
     2641 taacaaaatc gcacacaacg accccagagc ggtaacaatc atcaaccatt gccaacgttc
     2701 accaaagtct acttgataga taaaacgaaa gacagctaac ataatataaa tctctgtcaa
     2761 aacaatcgta aagcttgctg ccaaattagc gattgaaact aacgattgct ttctaggaat
     2821 caaggacaag cgaataccat ttgacgattg atttgcttgc tgatcctgta cattgcggac
     2881 cccccacatc gtcccgtaga gaatcgccat tccaatcaga gtgaagaaat aaaaactttt
     2941 gaaactcata ttacgacctg catgaacttc ttgaaaagat acttcttgtt caaaaacagt
     3001 catgatgggc gcgagatcaa cgttctccac ttctgccact ttactcgaaa ttataccaat
     3061 acgctgtaaa aattgatcca tgacgttttt catcaacgtc tgactcattc ctgcttgacc
     3121 cacatggagc gaaacagatg aaccatcaat gagataaaaa ccagccaaat catctgcatt
     3181 tagcttttca ttcgcttcta cttccgatag ttcctgtact tcaaataatg gtttatcatt
     3241 gacttcgact tgttctaaga ctgtggtaaa attttccgaa agcgctgttt gatccgcttt
     3301 gattacaatt ccagtattaa tcgtttcgag attgccacct tgatcaatct cgccaaaggc
     3361 cgccataaac atcaatccta aaagaattgg aaaggccaat gtccaaaaca acatggattt
     3421 atttcgcagc agtatttttg tgcgatattt ataaaaatgc caaaacatta tttcccctcc
     3481 tctagtctct caaatccttg ccagtaatct ctaaaaagac atcattcaag gacgcttctt
     3541 tggttgctat ttttccgtaa ctgatctttt cttcttgtaa gattgtaaga atcggcaaca
     3601 atactgtatt tagttcagta gttgttactt ggagctgacc ttttttataa gcgattccat
     3661 cagaagctat tgttcgctga caacacgttt tctgggagta atgggatgtc gatcgtcaaa
     3721 cgatccgccg tccgtaccat atcttttaga gattctttgg ttcctttagc aatcacctgt
     3781 ccttgatcca tgatcacgat ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt
     3841 gtatagacaa tggttgctcc ttgtcgattc agctccttga tactttctaa aattttattc
     3901 cgactttggg ggtctacagc tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg
     3961 gcaatcccac atgcgatgtt caaccgacgt ttcaagccac cactaagttc tcctggacga
     4021 aacgaaacat atttttctaa tcccactaat tgaatcacct cttcaatcaa ggctttcctt
     4081 ttctcttttt cggaaacata taaaccgcaa aaataatcga tattttcttg cacacttagt
     4141 tcgtcaaaga ccgcaacgtc ttgaggaacg accccaatca ttgccttgac agcataattt
     4201 tcaggagtca ttgttgtccc gaacaattta atggttcctt tgtcataatt taacaaagaa
     4261 agcatacagt tgatggcagt cgatttccca ctgccgtttg gtcctaacag acctaagact
     4321 tctccttctt gtacttgcag atcaaaatga ttcaatgcta caaaatctcc atagcgtttg
     4381 actaatttat caatttcgat aatcatcatg aaaaactcct tccaaatttc ttgttgatct
     4441 gactataata aagtgcaaga aaagaaacta gtgacgattg gctaaaccac acatgacatt
     4501 tgtcacaatg aaggagtctg ctatgcataa atctcggcta ctcaacgaaa ctagctttct
     4561 actggttatc tcattgcttt tgctggtaag tcaaggattc gttgctatgc aagttatcta
     4621 tttattgatc gccgttttat tcactggtgt cagttattta ctgcctacaa agccaaattg
     4681 gattttccaa gttattctat ttcttttatg tgccttttta cctgatcttg tcctgtttct
     4741 gccagcgacg atgcgagtga tcgcccctga agaaagccac cttgattact tgtttttgtt
     4801 aggttttttg ttgatttcga cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt
     4861 aattttgctg tttttttcct tttacttacg aaaaaaagat caacgaacga ccgatcttga
     4921 gaaaaagtac attgtctcca aagatactca ttgggaaaaa caacaagagt tatcggaaca
     4981 aaataaagca ttggccctgt cacaagaaac acaattagcc ttgcaaattg tggaagaacg
     5041 gaaccgaatt gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt
     5101 acaaattggt gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa
     5161 tttaagtgcc accgttcaca ctgggatgga tcggattcgt caaagtgtcc atgacctcca
     5221 tgagacatcg atctcttttc aacactcctt agagtttctg atcgctgatt ttccttcttc
     5281 tgttacgatt gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa actgcttctt
     5341 aatggtcatc aaagaagcct taaccaacag tatcaaacat agtcatgcca aaaccgttgt
     5401 tttacatttt cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg atggcaccag
     5461 cccacaaaaa aattctgctc ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga
     5521 acaaaaggga caagtccata ttttccaaga aaaccagcaa tttcagttaa cgatcatttt
     5581 accaagggag ggacaccaat gataacagta gtcattattg agatgatcca tttgtgacga
     5641 aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt
     5701 gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca
     5761 tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca
     5821 aagccatcct tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt
     5881 ccatcggtgt caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca
     5941 aatccgctta taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa
     6001 tgatgaagaa taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc
     6061 ttgcaatcat caaagaagta gctgccggga aaaacaataa agaaattgca gatgccttgt
     6121 acttgagtga cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac
     6181 gggatcgcac gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca
     6241 caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca
     6301 attgtcccgc aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg
     6361 attcgttgga ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc
     6421 tagttttgat tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc
     6481 tttttccatc tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa
     6541 tttatcttcg gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata
     6601 tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct
     6661 ttaaaatcaa gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat
     6721 catcggatct actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac
     6781 agcaggttct acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc
     6841 cactgtttcc attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag
     6901 cttttctttt acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc
     6961 gaccaaagaa tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt
     7021 atccatgatc cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt
     7081 gacataggct tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt
     7141 gtagagcaaa ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat
     7201 cttgaattca ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt
     7261 ccattcgttt cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac
     7321 tttatcttca gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa
     7381 tacattcaat tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt
     7441 gtcgttccaa tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat
     7501 tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc
     7561 ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat
     7621 ttgataagca tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg
     7681 cagacgtcct tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg
     7741 ataaatctga ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac
     7801 ttgaccagga ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt
     7861 cgtcggaatc gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc
     7921 aacaatcaaa agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc
     7981 attcagcccc agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg
     8041 tttagctgct cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt
     8101 tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac
     8161 agtagttttc cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc
     8221 agcgatagct tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg
     8281 aatattcttc tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg
     8341 ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt
     8401 ctcttcttgt tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc
     8461 aggctggatt tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt
     8521 ctccgccggt acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat
     8581 agctgctcga attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc
     8641 tgcacgtttg aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat
     8701 gatatccaaa gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa
     8761 cccgtaacga ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc
     8821 agcaagcatt tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc
     8881 agcaatgatt ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt
     8941 cttcccgatt cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt
     9001 tggcttttct tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac
     9061 taattctcca taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac
     9121 tacaatctct ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt
     9181 gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat
     9241 ttcttctggc tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt
     9301 cagttcctaa aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag
     9361 gctcttcagt ctctaaaatc gtcaaactac tattgaacaa tccacccatt tcacgcaact
     9421 cacttagctc ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta
     9481 cgaataaaag tgggccatct ttatgttggg caaccgcatc tgctacaaca gtttcgatac
     9541 gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat
     9601 gattacgcaa attcggcaga ttatccggat atttctggta catttcatcg atcgactgac
     9661 cttctaaagt acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac
     9721 gagcaagctc tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat
     9781 aaattgcttt aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac
     9841 tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt
     9901 gtgtcttccc atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt
     9961 attttactct ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt
    10021 ctaaattagg gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg
    10081 ataaattcaa tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt
    10141 tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct
    10201 cttctccatc agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat
    10261 gtagtttacg attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat
    10321 ttacatcatt ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac
    10381 ctagtggctc gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc
    10441 cagtgtttgc agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc
    10501 tcatatttat gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat
    10561 caactcggtg tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta
    10621 tcagaacagg aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct
    10681 caactttttt ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt
    10741 ccttttaata attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct
    10801 ttgattcgct cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt
    10861 tcctctggta tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca
    10921 gcaaacttgg ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa
    10981 aaagcctgac gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca
    11041 tcttctgaga gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc
    11101 gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct
    11161 tcggcagatt gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca
    11221 ttttgaatct ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca
    11281 tctaatagcg agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac
    11341 atgccagatt ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta
    11401 accgcatggg ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt
    11461 ttgcatatca agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac
    11521 agctgatgat tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc
    11581 cctcatttct gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat
    11641 acaatgccta gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg
    11701 ctgaaaacta cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt
    11761 gtgacaactt agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa
    11821 gttatgctgg ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact
    11881 tcgtctataa cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat
    11941 tagggacgga tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg
    12001 ccttagaact attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg
    12061 acagtattcc tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg
    12121 accgtatctt gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc
    12181 ttaaagaatt gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca
    12241 ctgcagagaa tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca
    12301 aaatcgtgga tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg
    12361 cttattgcaa atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt
    12421 gaaataaatc agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac
    12481 tgctggatca aatcctagac gatccattag catcgggatt aaactacctg caagattcgc
    12541 cactgtgatt gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt
    12601 ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt
    12661 caagacttca gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag
    12721 tcgacgaaca gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag
    12781 cgaaatgaag actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa
    12841 agaagcggtc gacataccta aaaacaacag agtgatcagc caaggcaaac gtcgagacgc
    12901 tgcctttact ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc
    12961 gctagcagct tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa
    13021 atgatcatca taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc
    13081 cacatcttct tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga
    13141 aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg
    13201 accttcttga ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt
    13261 cagcacgtac atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt
    13321 catgatcgcg ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc
    13381 gtctgccgac atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa
    13441 gtcaacagca ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat
    13501 ttcatctagg tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc
    13561 taattcttta ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg
    13621 gtagaattgc ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat
    13681 attattttcc cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc
    13741 atccaaacca atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct
    13801 gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga
    13861 tcataacaat gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca
    13921 tcaccgagca gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct
    13981 gtatgcagct ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca
    14041 gtctgtgccg aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg
    14101 gcgatcggca aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag
    14161 gcctgatatt ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca
    14221 aatccatgct ttgcaaaaag catcaaacca gatgtatccc gatctagtcg cgtcaccaca
    14281 tggattactt ggttttcgta accttgtttt ttataataag cctttacacg attagccatt
    14341 gtcccatttg gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc
    14401 agaacgtgtt catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca
    14461 tgttctcctt cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag
    14521 acattttcta cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac
    14581 agaccctttg agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc
    14641 tcataatccc atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg
    14701 ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata
    14761 aattgagcgg atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc
    14821 tgtattttcc aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa
    14881 agtacgaaac actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact
    14941 aggatggatc acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga
    15001 aatcgtcaat ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc
    15061 taccatcgta cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc
    15121 cggtgtttca tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc
    15181 ccggttcgtt cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta
    15241 aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta
    15301 gtgatggaac gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc
    15361 atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc
    15421 tatagtcttt gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt
    15481 cgctttcttt cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg
    15541 agaataaccg ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact
    15601 gataggcagc ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt
    15661 gatatttata attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa
    15721 tctggatttc cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca
    15781 ccagatgata agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga
    15841 gaactttcat gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa
    15901 actgacacat gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg
    15961 gaatatgccg taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga
    16021 caaattcaat cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt
    16081 tcactttcaa ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt
    16141 ccatagctga tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt
    16201 tttttcgtac tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa
    16261 aaggagtcga tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc
    16321 aagagtacga gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga
    16381 ctaatattta tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga
    16441 tccgtttcgt tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc
    16501 tcgaaacaac ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc
    16561 tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat
    16621 taatccaaac cggaaagctt acaacaaaac gggctgaatt ccctattgaa gaaggacttt
    16681 tagctattga tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtag
    16741 gggatgcatc tgtgggaaaa attgctttta taaatttttt aaaacgtttt tctttacctt
    16801 atcgtcctgc taagaacaag atccaacgta tgcttgaagc taaaaattag aaacctttca
    16861 ttttcacaag gcacttgagt tcattttttt acttttttct gttaagttat aggatgtgaa
    16921 agaatctagt cactagttgt atagaatcta ttttggggga gattcatatg atcgaaatct
    16981 atttatttgt taatccttta ggggaatact gtttcgaaat ggaacaacag ttattacaat
    17041 ttatcgaaga agagtacggg acaccatcaa aagaaaagat gcaatttcgc tttcttcctt
    17101 tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa aggaatttct cagaacaatc
    17161 ttgaggaacg taatcactta ttctcaatga cttattctgc agcattggac tgcaaagcgg
    17221 cgcagtttca aggaaagaaa aaaggccgcc gttttttgat caaattgcaa gaagcagtcg
    17281 gctgtaatga cataccatat tctcaagaac ttgctgaatc catatttgaa gaaatcggcg
    17341 gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa gtattctttt
    17401 ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca cccttctgca gtcgtttaca
    17461 attatgcctg cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca agtagccttt
    17521 ggagaatcac agagctgtgt aaaacaacag ataactcata tcaagttttt aatggtgaac
    17581 atttacatac aaatgattct cagttccatt caagaactag ccatttacac ttgctttcta
    17641 attaaaaaaa tacgtcgact cccttacaag agagccgacg tatttttttc gtttctattc
    17701 gagaaaatca aaaatagaaa gccttataaa cgatcgatca attcttctaa ttcatttaga
    17761 cgttgttcaa acatcgacat tgcatcttca atatatgcag cctgtgtcat atcaactcct
    17821 gcttttttca ttacttccac aggataatcg ctgttacctg cttttaggta agccaagtag
    17881 ttctctagtg cctctggttc ctggtttagt attttcttcg ccagtgctga agcagcagaa
    17941 aagccagtcg agtattggaa aacatagtaa ttataataaa aatgcggtat ccgtgaccat
    18001 tcgaatttaa tttctgggtc ttcttcgact gctggaccat aatattttgc attcaattta
    18061 ccataactat cacttaggta ttcactagtc aatggcacac ctttttcatc ttccgtatgc
    18121 atgaaatgct cgaattcagc gaattgtgtc tggcggaaaa ctgttccttt aaacccatcc
    18181 aagtaatggt tgagtacata agcccgtaca cgagggtctt tttctgtttc caataaatac
    18241 tccgttagga tattttcatt cgttgtcgaa gcaatttctg ctaaaaagat ggagtagtcg
    18301 ccgtacacat aaggctggtt cgaacgagtg aaataactat gaacactatg tcccatttcg
    18361 tggacaagcg taaatagctg atccagcgta tcatgccaat tcaataaaat atatggattt
    18421 gtgtcatagc ttcccgaaga ataagcatcg cttcgtttcc ctttattttc gacaacatcg
    18481 atccaacgtt cagagaatgc tttttctacg atggccatgt attcttcacc cattggtttc
    18541 agtgcttcta aagctttttc ttttgcttct tcgtacgtaa aggtaattgg agcttcccct
    18601 aagaccggtg tataaagatc atacatgtgc agtttttcca cttctaataa acgtttccgt
    18661 aattccatgt atcgatgtaa caaaggcaaa tgcttgttta ccacgtctac caaagtatcg
    18721 tatacacttt caggaatatg attattgctc aaagacgctt ctctggcaga gctgtaatta
    18781 cggactttcg ctttaaaatt atgtcctttt atatgtgtgc ctaaagtaga agcaaatgta
    18841 tttctaaatt gttcgtaaac actgtacaat cccttaaatg ctgcttcgcg cacccttcga
    18901 tccgtgcttt ctagcaactg accatacacg ccatgagata attggactat ttcaccattt
    18961 tctccttcaa tcgttggaaa aactagatct gcattattca aaacagcaaa tgtatcactt
    19021 gaagcatcaa agatttcacc tgctccagca agaagagatt cctgctcagc agataagaca
    19081 tgggctcgat tatctactat ttgctggata taatggcgat agacttccaa ttttggttct
    19141 tctttgaaat attgccaaat ctggtcatct gacaattgca atatttctgg ttcaaaccag
    19201 gaaacggctt cactaacttt cgaaaacaga ctgcttgctc ttgcataaag cgcttggtaa
    19261 tctgtatttc cagtgtcttg atcgttttta aggtgcgcat atacataaat gacttcagtt
    19321 tggcgataaa ccctcaatac gaattcaatc gcatttaaaa attgagaagc gccttgatca
    19381 agtgttcctt tgtgtttttc agattgtttt aactcttctg ataattccaa atatttctca
    19441 tcaaactctt ggtcgcttga aaagatcttg gtcaagtccc aagttaaatt ttcaggcaat
    19501 tcttctcgtt ttggcaactg ctttacttcc atgaaatcca ctccttttct tcccttagct
    19561 ctatctatca aaatagatca tagtctaaca tccacatgtc acagcatcta acttttctac
    19621 tcattcatgt gtctgcttcc atcaatttta gcataactaa atcttgtttt acatagaaaa
    19681 ccgattccta gcctattttt atacttacta ttttttcaaa gaagacaagt gtatgcattc
    19741 gttaaaaaac tgctgataga ccatctcttc atcaatcata ggaaaaagcc attccctttt
    19801 atgttctttt atttggcaaa gccatgagtt gaaatcagga actttctggt tttccaaggc
    19861 ttcttcgtac aacattcgat aaagaaaaac ctgttcttgt aaataaaaga aaaaccgact
    19921 tttttgatag atccacgatg atagatgcaa aagatgttta tgacgcaagt agcattgttc
    19981 ttgtatggat actgtattct tttgcttgtg aataagttgg cgtgtcagcc atttcttccc
    20041 atcttcactc actttttcta tttttttcat ttggttcatt ttaatttcgt tactagtgaa
    20101 tatttcttta aggtccctac tagaaaaagt ccagcagcta ctgcaaaagg aaatatgccc
    20161 tgatacagtc tctgtaatat gatgatacaa ataaagcttt tgtttcttga gatcagcttt
    20221 ccagcaatgc actcctcttt ttctattata gtagcagcaa cttttttcta tcaatgagaa
    20281 ttgcgattgt ccaagaaaag aattaccaag tatccaccat acttggtaac tgttattcaa
    20341 ataattttgg gttctttcaa taaatctctg gtgagacagt ctagaacatt ggatttctat
    20401 tgctagattc gccaacaaaa tatccggcct ttgccgcaac ctcggcaaat aggcttccaa
    20461 aaatactggt tctactgact gtcccagcca atccatgaag cattccttta actgtaagtg
    20521 ctcttttgat tctctttcac taaagaccga acagttatct gatttttgat gggcaaaatg
    20581 agcacatttt ttctttccct ttttcaaaat cactcttttt cgacagccac gacaataata
    20641 tttctttcct ttttctgcat gtttagcaaa acagcactta ttatattcat tcattgcaag
    20701 cagcattttt ctccctctga acttatttac gtctaagaat ttatttacac gaaaaaagag
    20761 ggcgtgaaaa agctattctg catcaagtaa taagaacagc taaacaaaaa tagttcccaa
    20821 tatttttcgt ttggttgggc ttgttaccgc agatgcaagc ttttgaacac atttatacga
    20881 aaaaaagact gcgacacaat ttttgtcaca gtctcttact ataatgctga tttatacaaa
    20941 ataaaatttc gttcgctcga tagcattacg cgccatcaga cattgtccat attcattcaa
    21001 tgtttctggt gtacgatcgc tcttttcagc aaactccaaa attcgagcaa tctgattctc
    21061 tacatccgct tggttgagat tttccatcca gaagtgaacg actaggtaat agcgctcttc
    21121 gaataaatac aaatcagtcc aagcagactc tagttcgaca tctgttgcaa gttggatcac
    21181 ttgttcaaaa tcttcaaaag caaaagttgc ttgtctttcc tgttcttcta gggcgcttcc
    21241 agataattct tcctccggat cgctgccaat aatctgctga cgaagaaagt cactcacatc
    21301 ttctgaatca acagtagaat cttctgattg agaaatttct tcatccggaa gatttttact
    21361 gataaacagt tctaatccgt ctccttttgg caaaacttgg aaagtgacag cttcgcttcc
    21421 tcggaactcc tcttcgatat caacttcttc caaaatacta taaaagaaat tttcgatttc
    21481 tttgtgattg ccaagtaaat ccaaaaatga aacgccccgt tcctctaaat cttcatgacc
    21541 aattagtaca cggatcgtat tttcattaat atgttccatt tccattttgc tacacctcac
    21601 tttgccacaa tgtttaatat agattcattg tagcgaaatc agcttaaatg taaagaaaaa
    21661 aaatctcgct gttaagcgaa tttaataaaa ccagcggatt tttcaacctt tcaagctatc
    21721 ttatttaact ataacatttt tggcagacga atactatata gccgactttt cagctaattt
    21781 tccctctcta tttttcgtgc aatca
//
LOCUS       DAYYAR010000041        21706 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000041 DAYYAR010000000
VERSION     DAYYAR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.041"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..2728
                     /locus_tag="ACHAT0_001299"
     CDS             53..2728
                     /locus_tag="ACHAT0_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM8451251.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGIAKIEATFVEAQKKIWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2910..3149
                     /locus_tag="ACHAT0_001300"
     CDS             2910..3149
                     /locus_tag="ACHAT0_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451252.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLAFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNGRVAKEWYLVHS"
     gene            3165..4538
                     /locus_tag="ACHAT0_001301"
     CDS             3165..4538
                     /locus_tag="ACHAT0_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8451253.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGGKADRPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLAYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEAHEAKVVRHIFQLYLTKKYGYKKLCQRLTQQKFFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKTIFLQAQEIRQSRCTAKKDTYPYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKHKVIQQKKQEAQWLKAFEEGNCSLEELKQQLQALQ
                     KPRSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDHVCRIEVTKEKTVCGIYFRQMTQ
                     NILEGNE"
     gene            4542..6179
                     /locus_tag="ACHAT0_001302"
     CDS             4542..6179
                     /locus_tag="ACHAT0_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8451254.1"
                     /translation="METTPTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMSYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPKPILVKGTHEPIISEEDY
                     QKVQERLVLEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQQGEPIQVKDVQRILTSLDRFLAQSEKKQVKALYRTFIEKITFDPNYKEILEITM
                     KFTPAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6342..7274
                     /locus_tag="ACHAT0_001303"
     CDS             6342..7274
                     /locus_tag="ACHAT0_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451255.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNI
                     NYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
     gene            7390..7710
                     /locus_tag="ACHAT0_001304"
                     /pseudo
     CDS             7390..7710
                     /locus_tag="ACHAT0_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            7888..8247
                     /locus_tag="ACHAT0_001305"
                     /pseudo
     CDS             7888..8247
                     /locus_tag="ACHAT0_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            8311..10968
                     /locus_tag="ACHAT0_001306"
     CDS             8311..10968
                     /locus_tag="ACHAT0_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8451256.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            11040..11516
                     /locus_tag="ACHAT0_001307"
     CDS             11040..11516
                     /locus_tag="ACHAT0_001307"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8451257.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            11530..12318
                     /locus_tag="ACHAT0_001308"
     CDS             11530..12318
                     /locus_tag="ACHAT0_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8451258.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            12320..13117
                     /locus_tag="ACHAT0_001309"
     CDS             12320..13117
                     /locus_tag="ACHAT0_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8451259.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            13136..13531
                     /locus_tag="ACHAT0_001310"
     CDS             13136..13531
                     /locus_tag="ACHAT0_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8451260.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            13533..14654
                     /locus_tag="ACHAT0_001311"
     CDS             13533..14654
                     /locus_tag="ACHAT0_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8451261.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            14674..15543
                     /gene="scrK"
                     /locus_tag="ACHAT0_001312"
     CDS             14674..15543
                     /gene="scrK"
                     /locus_tag="ACHAT0_001312"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8451262.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            15744..16253
                     /locus_tag="ACHAT0_001313"
     CDS             15744..16253
                     /locus_tag="ACHAT0_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFM8451263.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16353..17003
                     /locus_tag="ACHAT0_001314"
     CDS             16353..17003
                     /locus_tag="ACHAT0_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451264.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17454..18392
                     /locus_tag="ACHAT0_001315"
     CDS             17454..18392
                     /locus_tag="ACHAT0_001315"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8451265.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18405..19457
                     /locus_tag="ACHAT0_001316"
     CDS             18405..19457
                     /locus_tag="ACHAT0_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8451266.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            19723..20358
                     /locus_tag="ACHAT0_001317"
     CDS             19723..20358
                     /locus_tag="ACHAT0_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8451267.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            20549..21340
                     /gene="msrA"
                     /locus_tag="ACHAT0_001318"
     CDS             20549..21340
                     /gene="msrA"
                     /locus_tag="ACHAT0_001318"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8451268.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7380 a         3234 c         4421 g         6671 t
ORIGIN      
        1 gaggtaatgt agtcatacaa ataaaaattt tttagaaagt gagtggtaat taatgaaaat
       61 gaatcacact tcaattcaga agcatttaca actgttaaat acactgggga tttcttatgt
      121 taatttgaga actttttcta cattaaacaa gaaaaccgtt tctcatattt tttctactgc
      181 agattctcaa gcaattatta actatttaga aaaacagaaa ttgtctgaag gtacagtaaa
      241 cataacttat aatcctatta aacaagaggt gattacacaa aaaaagcatt ctgtgagaga
      301 caatgatatt gaaaaaattc ggtttgtatt tattgatatt gaccctaaaa ggaaagaggg
      361 aagtgcaaca gatagcgaaa agaaaaaggc agaaaatgtt atggaacaag tagaacgtta
      421 tttgaaagaa aagagaatag aatcatttgt aaaagttgat agtgggaatg gctaccatat
      481 ttttcttccc ataaatgaac aaccaaataa tcacgagacg attcttacta ttcagaattt
      541 tcttcaatta cttcaccgaa gatttggagt agaagatgaa gttgatattg atacttctgt
      601 ttataatcct tcaagattat gcaaattagt tggtacacca gcggtcaaag gaaagtccac
      661 aaaagagcgt ccgcatcgtt tatctagttt cctttctatt cccgataaca tagatagaag
      721 taattgtttt aaaattcttg aacagatagt tgaagaaaat tccatgatgt caacctctag
      781 agtaaaaagc catccatctt ttagagaagt gttacctttt attcaagcag actataaaca
      841 atggttagct cactatcctg aattaactta taccataaaa gagggcgatc ataaaggagt
      901 taccttactt atatttgacg aatgtccatt aagaaaacac acgaataatt ctaatggttc
      961 ttgtttgtct gttacggctg ataataagat tcgatttcat tgtctgcatg cttcggataa
     1021 aaataaagat attcaagact ttgtaaaaaa atatccgttg ccaaaacctg taaatccttc
     1081 tgtagaatca ttagaatcca atcaagaata ttattttgac cagtttaaac tgaatcaaga
     1141 aggagtattt ttggtgaaag aaggtcaaga agaaaaagtt gcttgtccga tgtttattaa
     1201 gcaaatacgc aaaaatatcg aacaaaaagt cattacactt gttcttgcct attatgtcga
     1261 tcattgttgg gatgaaatag aaatagaagg tggatgttta caaactaata attttaaaca
     1321 attagcaaaa catggcttaa tcattaaagc tagaagagaa gctgaagtcg ttgacttttt
     1381 acagattcaa cgggagaatg ctccaatctt ttttgaacat gaatctcttg gctggaaaaa
     1441 agaaaatgaa gcaatgattt ttcagttaca agattctttt ggacgtgaaa ttccttctgt
     1501 attaactaaa aactcatgct accaattaac aactaatggt acagaagcgg aatttgacgc
     1561 attggttcat aagtatgttt taggaacgaa cttagaactt gcttggtcga ttggtattgc
     1621 ttctattgtg ttaggctatt taaacgttac gaaggctaat acttttttga gtttactaat
     1681 taacctacag ggcaagagca caacgggtaa aaccactacg ttacacttta ttgcctcttt
     1741 atttggaaat ccaactacgg ttcttagaaa tatgaatgct accaataaag ccattataaa
     1801 gctagcatca aacaatcagg gaggtgttcc actaatacta gatgagttag gagcagcaac
     1861 tcaaaataac tttagctcgt tgatctatca attagcttca ggagaagaaa ggctaagatt
     1921 agattctaat tgtcagctta tgccacaaga gactttttct gttttgaccc ttacctcttc
     1981 tgaagagcgt ttggaaaatt atttcaatga gaaattaact ggtttgaaag ttcgatatat
     2041 cgaattttct gatatcaagt ttacaaagga tgcgaatcac gcagagaaag ttaaaagtct
     2101 ttcttctaat acttttggtt ggggtataaa gttatttctt ttgaaactct ttgaacaagg
     2161 gattgcaaaa attgaagcaa cttttgtcga agctcaaaaa aaaatatggg ctgttttacc
     2221 tgaacattct ttgaagcaac gcattggtaa taatctagct ttgatttata gtggagctct
     2281 cctaattcaa gatatactgg gctatccttt acgattagag catatacaga cggtgcttat
     2341 taattcgtac gaacagttaa ttgtagatac tagtttagga agtgagcaaa cggtatttga
     2401 ccgagtgaaa acgttgttgg tgatgaatag ccataaattt ttaacaaaca gtgacaacaa
     2461 acatacgccc agtacgattt ggggaaaagt atcaattcaa aaaaatgggg atattaaggt
     2521 aaatattttt cctatcccgt ttgaggactt gttaaggcga gaatttcaag taaaggatct
     2581 aacctatctt tttagacaat tgcttgattc gggtaatatg tgtgcagaaa aagggagaag
     2641 aacgaaacgt attcacatga atcgtcaagt tcttccaaca tacgagattc tattaccttc
     2701 ttcactcaaa tcgtattttc gattgtaatt tagaataaac caaagtatga aagtgaggca
     2761 ccatgttatg gaaatatttc ttattgattt agcaaaacaa ctgattactt atctgtatga
     2821 tgacgagcaa acccaaacgg aagtaaatga agcttgatct atgcttcatt tacttcattc
     2881 aatcaaacat ttaagaaagg ggaattctaa tgactaaaaa taaaatgaca ctaaaagcag
     2941 aggttcttct gtatattcaa gaacattttt ctaatcaagc cttttttaca aaaccaattt
     3001 acttggcttt cgaaataaga ggggtatctg ctggatctat tggaggtact cttcaagcgt
     3061 tgaagaacga aggatattta gaaaatcact ttgtgcaacg ttcgttcaat ggaagagtcg
     3121 cgaaagaatg gtatttagtt cattcataga gaggtgattc aagcatgcgt gtggcttgtt
     3181 atgatcgaac gtctgtttat gaagaagcga aaatcagtaa tgacgctcaa tatgatgcag
     3241 tgaaagagta tgttttagag aaatatgaac aaattcctca acgattttca gacaaaggag
     3301 aatcaggtgg aaaagcggat cgtccagctt ttcgacagct tagagcttgg gtttgtgcgt
     3361 cagaagaaga gggggtgatc tatgtctggc gttatgatcg attggcacgt caagccaaaa
     3421 tagccttaga atttattgag ttgtgccaac aacatcaggt tcaagtttat tcgatttctg
     3481 aacctcttcc tgcttctttc tcgcacgcgt caacgaataa actatttgtg caattattgt
     3541 tcacttttgc agaacaacaa cgaaacgtca ttattgaaaa tgttcgtgcc ggacttgcct
     3601 ataaaaagca acaacggcaa tatttgtctt ctgcggttcc atatgggtat cgtttagtga
     3661 atgggaaaat ccaacaagaa gcacatgaag caaaagttgt ccgccatatc tttcaacttt
     3721 atttgactaa aaaatatggc tacaaaaagc tttgtcaacg attgacgcaa caaaagttct
     3781 tcttccgtga acgtcctttt cagccctatc atatttatag tatcctaaaa aatccattgt
     3841 actatggcga aatcaaagga ggatctttgg ggaagtatct aggaaccttt gaaccgattc
     3901 tctcgaaaac aatcttttta caagcccaag agattcgcca atcaaggtgt acagctaaaa
     3961 aagatacgta tccgtactta ttacgtcaaa aaattcggtg tcccttctgt ggtcgtcatc
     4021 tgagttcgaa gtatcaatgg aatacaaaga agacgaagac gcttcattat tatcattgta
     4081 ccgaccgaag ctgccaagga atctttcttc gggcagaaag ggttgagtca gaagtattgg
     4141 aaaagataca ggcttttttt caatacgaag gcgtctatca agccttgatt gcccaattga
     4201 cgcaacaatt agccgaaaag cgaaaagatg aaaaacacaa agtaatccaa caaaagaaac
     4261 aagaagcaca atggctcaag gcatttgaag agggcaattg ttcactggaa gaactcaagc
     4321 aacagctgca agctcttcaa aagccgaggt ctacttcttc gtttctttca ctccaacagt
     4381 gtgaagcaaa agtgaaagaa tggcttcaac taaaagacct ctctagtcga caattgttat
     4441 gtgaccatgt ttgtcggatc gaggtaacaa aggaaaaaac agtctgtggg atttacttcc
     4501 gacaaatgac acaaaatatt ttagaaggga acgaatgatc tatggaaacg acaccaacca
     4561 aacgagtcgc attgtatatt cgggtgagta cccaagaaca agctttagaa ggacatagta
     4621 ttcaagcaca agaacaaaat ggacgacagt ttgccgaacg gatgagctat gaagtggtag
     4681 aagtttatgc cgatgaaggg attagtggga aatccacaaa aaatcggttc gcctatcaac
     4741 gaatgatgaa cgatgctcgg aaccatcgtt ttgatctact agtgatttgg aaattgaccc
     4801 gtctcggacg caatatgtta gatgtcttac aaaccgtcga ggaactgttg cgatatgatg
     4861 tggggttaca ttcgatctcg gaacaatttg acgtgactac ttcgacagga aaactaatgt
     4921 tacaactcct tgggagtttc ggtgaatttg aacgcaacca aattgcggag aatgttcaga
     4981 tgtcaatgca aagcttagtg aaagataaaa agcgttatgc aggtggacga cgtttaggct
     5041 atgtcagtgg tattgacacg aatggtcaaa aacaattggt cattgaacca gaagaggcga
     5101 aaattgttcg tctgatctat gcaaaatatt taggcggagc agggtatcga gcgattgcca
     5161 attttttgaa tcgtcaagga tatcaaacag tcaaaaagaa tgctttttct accacggcag
     5221 tcaaagatat cttacgcaat aaaatttatg cagggtattt agaatatgca cgctatatca
     5281 attgggaaac caaacgacga aagggcaaga acccaaaacc gattttagtg aaaggtaccc
     5341 atgaaccgat tatttcggaa gaagattatc aaaaagtgca agaacgcctt gttttagaag
     5401 gcaaacagcc caaatggaac catacaggtg aaaatgtatt gacaggcttg ttacgttgtc
     5461 cagaatgtgg ggcaccgatg gcagcgagta atgtcacaaa tactctaaag gatggcacaa
     5521 agaaacgaat tcggtattat tcttgttcag tctttcggaa taaaggagca agtgtgtgtc
     5581 atgccaattc gattcgtgcc gaacaagcgg aaacatttgt cgccgaccga ttaaaggaga
     5641 tcgttcagct accagaagtt cttccccgat tggtggcagc gttaaatgaa gaaatagtgc
     5701 gacaaagcca accattggaa caagaactcg tggtgttact ggaacgaaag gaagaattga
     5761 agactaaaat tgaaaaatgg gaagctgctt tggaagatag tcctgaattg tttccgatgt
     5821 taaaagaccg cttagacgaa ttgactgaaa aacgacgaca gttgcacatt cgagaaaatg
     5881 aaatcttagg gatctttcaa caacaaggag agccgattca agtcaaagat gtccaacgga
     5941 tattgactag tttggatcgt tttcttgccc agagtgagaa gaagcaagtc aaagccttgt
     6001 atcgtacctt tattgagaaa ataacctttg atcctaacta taaggagata ttggagatta
     6061 caatgaaatt cacgcctgcc gtggtacaac aattgaatga gcaatatcaa atagccgtat
     6121 ccaaaacaaa ggatacggct atttttgtgt taaagacacc ctttacgtta acgatctagt
     6181 ctaggttata tcaaacaacg ttagaggggc gctaacgctt ctctagcgat ttgaaatcgt
     6241 tagatgagtc gttagggagg tccttatcat aaatagggat atttattaat gaatatttga
     6301 aatataattg tatataagaa ttaaaaagag tggggataga tatgaaaaag atgggtaagt
     6361 gtattttatg tgaaaaatac actgaactta ctaaagaaca tgtaccacct aaaaaatcag
     6421 ggaatactgg aggtaaaaag accagaactg gtaatttaga tgatttcctt aagagcgatt
     6481 ttacaaaagg tgattttccg aaggggatta aaaggaaacc tcaaggaaat gtttattata
     6541 cgttatgtag taaatgtaat agtttttttg gtagtgagta tgttgaggaa tatattagat
     6601 ttgcagaaga caataaaaat ttcctttata acaatacaag tttaaagaat ggtaagtctg
     6661 atttaacaca ttcaataaag aaaatgaatt cacttagagt tgcaaaagca attgtagcaa
     6721 tgttttttag tttaaatggg gatgaagatt caatggataa gccattctta gattcggtac
     6781 gagaatatct tagcaatcct aaaagtactt tatttcctca tgaagactac aaaattataa
     6841 tgaattatta tcttccaata ggtgctccac agaattctaa aatcaaaatt cctaatgaca
     6901 ttgtaaatga tggtgacact cacaatgtaa taaaaggttt tatgcccctt gatctatcaa
     6961 ctgggagaaa aattcgattt tcagaaattc agtatgaagg aattggtttt actcttatag
     7021 atttaaaaaa ttcaactttt gaaataaaag aaggattaga tttaaaaaat ctcctttgtg
     7081 cagatgataa aatcaaaaaa atacaattgt acaaagtccc tatattaaca ccaacagtac
     7141 ttacaatgtt ggagatggaa aataatatta attatattga ctcaggttca gatttgctga
     7201 ttaggtggca ggtaataact aattttctta tggaattaaa aaaatacagt aatactcaaa
     7261 cgaagttctc ttgaagttat aaagaataca ataaatataa atttccttta gactatatta
     7321 aaatcagcaa tcgaaggaaa ataaacgaag aaaatggtac gcaaaatcta actattagag
     7381 caaaaacagg aagctgcttt ggaagatagt cccgaattgt ttccgatgtt aaaagatcgt
     7441 ttaaatgaat tgactgaaaa acgaagacag ttacaaattc gagagaatga aatcttaggg
     7501 atctttcaac aacaagggga accgattcaa gtcaaagatg tccaacagat attgactagt
     7561 ttggattgct ttcttatcca gagtgagaag aagcaagtta aagctttgta tcacaccttt
     7621 attgagaaaa tcacctttga tcctaatcag aagtagatat tgccgatccc gatgagattc
     7681 acacctactg tgatttgata attgaatgag taatatcaaa tagccgtatc caaaccaaag
     7741 gatatggcta tttttgtgtt agggatatac gtcacgtagc ttgttatcaa gaataagttt
     7801 ctttattttt ttgctttttc taaaactgag tatgctaaaa taagagggta tatagcaact
     7861 ttaaataaga gataggagtg tatctttttg ggtgcagaat taaatcagaa gttatttagt
     7921 gcggcagata atctccgtag taaaatggat gcgtcagaat ataaaaatta tttattgggg
     7981 ctgattttct ataaatattt atccgaccgt ttgttggaac aagtggttct tttagcagat
     8041 gaatccttag aggaatatga cacagtaagc aaacaaacga tgttgtatcg tgaattactt
     8101 tcagatgaag aatcaaaaga agatctgatt gcaacaatcg ttgatatttt ggggtatgcg
     8161 atttcgccag agtatctttt caacgtctta gcggatcaag caaaacaagc aacgttccaa
     8221 ttgaatgatc taaacaaagc ctttttagca agtcatattt atggaaaaat actgtcggca
     8281 aaattcgaat agtgtcgagg aggtgctgtg atggaagaat taaaagaacg cattgtcaat
     8341 tatgtcatag agaactctga gattaaaaat aaatggggga acaatacagc acagaaaata
     8401 tctgaaaaat taaatgttag tcgtaatatt gtctctcaat atttgaatga attcgtaaaa
     8461 gatggagaat ttggaaaaat caatactcgt ccagtaatct tcttttcttt accggagaaa
     8521 gttccaaatc aacaaatttt tagctcttca gaggaagcgg aaaaatggta tgatagctct
     8581 tttagggttg gaatagaaag ggttatcgga agtcaaggaa gtctgcgaaa tgtaattgct
     8641 caaattaaag cagcaatcta ctatccgcca aatggcttac ccattttact tcatggcgag
     8701 actggaactg gaaaaagcta tttagcaaat tatgtgtata aggatttgtt ggccaataat
     8761 tatatagaag agaaggcatc tttcatttca gttaattgta gtgaatatgc caataatcca
     8821 gaacttttct tgtccaattt ttttgggcat gtaaaaggtg catatacagg agccgaagat
     8881 ggaagaaagg gtttagcaga attagctaat aatggagtct tatttttaga tgaaattcat
     8941 gcattgagca atgaatgtca ggagaaactt tttcagttta tggatacgag gaaatttcat
     9001 cggatgggtg ataatgaaaa ctggtacaca tcaagttgct ggattatatt tgcaacatca
     9061 gaaccacctg aggataaact attatcaact ttgttgagaa gaatacctgt aaagctaaca
     9121 cttccaacat tagacagtcg ttctctttat gaaagaaaac agttaatcaa gatatttttt
     9181 aaatcagaag aagaaagact agcaaagaaa atactgatat ctcaacaatt attagatttt
     9241 ttattaaact atcgctttag tgaaaatgtt ggcaatttga aaaatatcat tcaattgacc
     9301 tgtgccaatg ccttttattt cttagatgat ttggaagaac tttatattca gatgtatcat
     9361 ttaccacaat gggtgaattt caacggcatg aaggttatga aaagacagaa tgaattaaaa
     9421 tatattcaaa tggatgattt ggaaaaagta gacgaagttt ttaatatcaa cgaacttata
     9481 gagtatcttt atcaaacgtg gaagaaagac actgataagc agcagctttt tgagaaatat
     9541 gatagatatt ttagacaaaa gaatgttgat atccaaaata attcagcttt aacatatccc
     9601 acctatcaag agattcgatt tagacagtat gttaaggaaa tagagagaga ttttcaaata
     9661 gaatttgact attattatct tcaaataacg tctatctttt tgaattcttg gatttatcaa
     9721 atccaattaa catcggacga taaattatat tccttcatca atgatattaa tagttggtgt
     9781 agtcgagaag ttcaattagt caatcgtttt attcaaatag gggatattta tttaacaaaa
     9841 attcaaaaaa atgttttggt tattttattt aagttaatgt gtagtaaaga atttcagcaa
     9901 aaacgtttag cactgatttt ggctcatgga gatctaacgg caacctgtat agcaagtact
     9961 gctaatagga tcgttgagag atatatatac gacgccattg atatgccact aatgacttca
    10021 agtagtgaga ttgcagacaa aattaataga tacttacaag agtgtaccgg atatgaagaa
    10081 attattttac ttgttgatat gggttcactg gagaaaattt atctaaattt ggaactagac
    10141 gctcaaaaaa aattcggact actaaacaat gtttcaacaa gaatggctat tgatgttgca
    10201 aatcagatat tattagattt accgcttaaa cagattcttg aacaagtaaa agaaaatcaa
    10261 aatgtggatt atatttatga atcatcaaat attaagaagg atatgattgt ctgttcttgt
    10321 gcaagcggat taggaacttc gtttaaatta aaaaagatac ttgaagatag ctttccaagg
    10381 gatactgatc ttgtcataga aacgtgtgac tattactctt tagtgaatga aaactattta
    10441 tcggagcttc gtaaagagaa caatattctt tttattgttg gaacactaaa tccacatcta
    10501 gaagatgtag aattcttttc aattgaagat atgattttag gaatgaatat aagtaaattc
    10561 tccaatcaat tgaatagaca ctttaatgat aatgaactga aagttttgaa caataatatt
    10621 ctgaagaatt ttagtctaac gaatttaatg gagcaattga ccatattaaa tccgactaaa
    10681 ttattagaac gggtttcaga tgctatatac atcttacaaa atgatctagg aatttcattt
    10741 gataataata cgtgtttcgg attatatgtt cacattagtt gcttaattga acgattagta
    10801 aaacaaaata ctttggaaga tgaaatttat ttcaatgaaa cgagtgagga atttcaaaaa
    10861 tttcagacac attttaagca atctttcagt gtcgttgaac attattatag tgtcgacatc
    10921 ccgattcatg aggttaaata cgtttatgat tatgtaaaaa gagcttgatt tatcaagttc
    10981 tttttgtttt ggcatggtaa ttgcttgtaa gaaaatgaaa gataaaggag gaagtattta
    11041 tgagtattgt tatgtcgaga gttgataacc gtttattgca tgggattatt gtcacgcaat
    11101 gggcaccaat gtctaaagca aacagagtga tggtgatcga tgattctgtc gcaaataacg
    11161 acatccaaaa atcaagtatg cgtttagcta gacctgctgg aatggctgta tcaattatca
    11221 ctgaagaaac agcgattgcg aattttaaaa aggggaaata cgatcaggaa aaagtatttg
    11281 tcatcgtgaa agagccggaa acaatccttc atttagtaga taacggtatt agaattgacc
    11341 gactaattgt tggcggtact gttgttaaag ataatgcatt acagctatct tctagagcct
    11401 atgcatcaga tgaaaatatc agcacgtata aaaaattgtt ggattctggt gtagaaattg
    11461 ttacacaatt tgttccggcg gataaggcac aggaaattca atcaaaatta ggataaagga
    11521 gtaaggataa tgcattttac tatttttcaa attattatgc tgactatatt agcatttatt
    11581 aaacatgttg attattatgg gattccgatg atctttgtaa attacgcagt tttttgggga
    11641 ttattcacag gaattttgat gggtgatata aaaacaggtt tgatagttgg cggtacagtt
    11701 caattgatgt cattaggagt tgccggtttt ggtggctcat ctgtaccgga ctatggaacg
    11761 tgcgcaatta ttgcaacggc atttggtagc cagcttggag tagaagccgg attagcaata
    11821 ggactacctg taggtatgtt aggggtgcaa ctagatgtaa ttgtcaaaat cttaaatggt
    11881 tttgttgttc gcaaatcaca ggaatatgcg aataaacgag aatttaagaa aatgaatagt
    11941 gttttgtggt tggggccaat tttctttggt ttatctgcag caattccagt atttattgcc
    12001 gttactttag gacagtctgc tgttaattac gttttggatg ttttacctaa ttggtttatg
    12061 gatgggctaa cattagccgg aaaaatgttg cctgctgttg gtattgcgat gttgttacgc
    12121 tttatgccta caggtcgata cattagttat ttacttgttg gatttttctt agccgctttt
    12181 ttgaaggtac ctattattgg agcggcgatc ataggattcg ctttagcatt taatgtgtat
    12241 catagagctg aaaaagatgc acaaatcact gcttctgcgg gagcttctga tgttgggttt
    12301 attggggagg atgaataaaa tgagcgaaaa atatcaagta acgaaaaagg atcttaggaa
    12361 agcgaactat cgttggttga tgtcagtttg tacctttaat tatcaaacac agcaaggtgc
    12421 atctgtgact tacgcattgt cacctatttt aagaaaatta tacaaagatg atgatgaata
    12481 catcgaagct ttaaataatc actttcagta ttacaataca cagccttggc tggcagccat
    12541 tattttaggt gcttgtgttg caatggagga aaagcaagga ttagaagcaa aagatgcgat
    12601 taatgacttt aaagttggta cgatgggtcc aattgctggt attggggact ctcttttgat
    12661 gacgatgatc ccaaccatca tgggatcaat tgcggcttat atggctttag aaaataatcc
    12721 tgtaggaatc tttttatggt tcattttaat catggtaatc ttctttttcc gaatgagagg
    12781 ctttgaattt ggttataagc agggaatgaa gattgttact gaatacggtg aaaagattaa
    12841 ttatttaact gaagctgctt cagtattggg gctaacagta gttggatcat taattgcttc
    12901 agttatcagt gtttcaacgc cacttaaatt cacttttggt gaagttagta tgaaaatcca
    12961 accaatgttg gacaaaattt taccaacatt aattccagta attattactg ctgcagcata
    13021 ttatctactc agtaagaaaa aaatcagtat gaccattttg attctactcg ttattgtttt
    13081 tgcgatggtt atggctgcat ttggaatatt agcgtaaagg aaaaggattt gaattatgag
    13141 aagattgatc attgctagcc attcaatact agctgagggt tttaaagaga cactagagtt
    13201 tttaacaggt atgcataatc aaattagtag cttatgtgca tatgtcgaag aaattgatga
    13261 tatagatgaa caaattaaag agttagtgga tactacagac gaaataattg ttttaactga
    13321 catggctgga ggaagtgtaa atcaaaagtt ttttacctat atttctgaca gagtgcacgt
    13381 aattgcagga atgaatcttc ccttggcaat gtcaatcgct ttgcagttag gaaatgataa
    13441 tttagatatt ggagcaatta ttgcggaggc acaacaacaa atcatctata tgaataatca
    13501 aaaaagtgaa gaaaggatag aggacgaata aaatgaattg tttaaataat atatggctcc
    13561 acaagggaga ggaaagtaga actatttcac cagaaaataa aaatggtgaa aaagggaaag
    13621 cggcaatgac agcgagtcat ctaggaattg ggagaaaagg gtcaccttct gttccgataa
    13681 atgctggaga gacaattact ttagcagaaa tggaaggccc tggaatttta cgtcattttt
    13741 ggatgacagt gacagacaaa acaatcgctg ggagtttcgt cttacgtgat ttaattttgc
    13801 gtatttattg ggatgatgaa tccactcctt ctgtagaaag tccattagga gatttttttt
    13861 gtaatgggtt tgggacaaga tgcgaagtga attccttacc aatcgtagtg aatccaacat
    13921 cagggatgaa ttgctatttt gagatgccat ttcgaaaaaa agctaagatt actttaacga
    13981 atgaacatcc tgagtttatt aagcacattt tttatacatt taattatgca ttgactgaag
    14041 taccggatga tgcgatgtat tttcatgcta aatggaaccg tgaacccatc acgacaaaag
    14101 gaaaagatta cacccttatt gatggaatca aaggcactgg gtattatgta ggaacctact
    14161 ttgctttgtg cgcattggaa agatactggt ggggggaagg agaatttaag ttttatatag
    14221 atggagatga agatttccct acaataacat ctacaggagc agaagattat tttggcggcg
    14281 cttgggcttt ccataaaaag aactttggag aaagggcata tgctcaaaat tttaatacgc
    14341 tatttatggg ctatcctttt caatctaaaa gagatcacac ccgggattat tttagtgctg
    14401 gaaaacctaa tccaaatcca gttcatggtt ttggcgatga tgctttaccg atgcatgggt
    14461 tgtaccgctg gcatttaccc gaccccatta gtttcaaaga aaatttacgt gttactttcc
    14521 aaaccctagg aaatgatgac atcaagcttt atgagcgtca agacgatatt tcttctgttg
    14581 cctattggta ccaaaaggaa ccacatgagt ttttcgaaaa actattagac agggaagaac
    14641 gaattcctag ataaaaggac ggtggagtaa gaaatgtatg gctcaataga agcaggtggg
    14701 actaaatttg tttgtgccat tggcaatgat gatttaaagg ttttagaaag agtgagtttt
    14761 cctacaacta caccgaatga aacaatgagt ttggtaattg atttctttaa tcactataaa
    14821 gaacaattag aaagcattgg agtagggtca tttggaccga ttgatatcca tcgtgaatca
    14881 aagacctatg gacatatcac ctctactcca aaaactgcct ggaaaaattt tgattttgtt
    14941 ggaacgttga ataagcattt cgaaattcca attgcctgga caaccgatgt gaatgctgct
    15001 tgttacgggg agtatgtttc aggtcatgga aaaggtttat ccagtgttgt ttattacact
    15061 gttggaactg gaattggtgg aggagctata caagatggga tttttgttga agggtttagc
    15121 catccagaaa tggggcatac gttagttaag agacattcag gagatacttt ctctggtact
    15181 tgtccttttc atcatgattg tctcgaaggg ttggcatctg gacccgctat cgagatgcgt
    15241 acgggaacca agggacaaga tctttctata gaagatccgt tctgggaaat cgaggcggat
    15301 tatattgcac aatgtgcctg taatacgacc ctgatgcttt caccagatat tataattttt
    15361 ggtggtggtg tcatgcaaca agaacatctg aaaaagaaag tacaaagacg ttttttagaa
    15421 ctaattaacg gctatgtcga tacgccaaat atcgaagaat acattgtcac acctaagtta
    15481 gcagataatg caggaactat aggatgctta actttagcaa aagatgttag aatcaattct
    15541 taagcaaaaa agtattagtc aataaaatgc ttatgaagtc cgatcaactt aatgtggtcg
    15601 gactatttgt attcagcaat atcattcact catgataccc agcgccttat cataagtgat
    15661 gcggttttta tcattgatcc taaaaatctt agcaagacaa aaagtacgta agaaagtaaa
    15721 gactaaatac ttcggaggaa caaatgaata acaatgatag gttattgcga ttgcgttatg
    15781 cgattgattt gaaagatacg gatttgatta aagcttttga gctaggcggt gtaccactga
    15841 ctaaagaaga agctcaggcg gtactgacca aagtacagga taagaacaaa gataatgcag
    15901 aaaataatgt gtatgaaaaa accatcaaca accaagtttt tgacgcattc ctaaatggcc
    15961 tgattctctt ggcgcgtggt ccgcaaaaag ataagccgat agtagacacg ccgcaaaaaa
    16021 gcaaggaaat caaatatatc aacaatgtgt tgctaaaaaa attaaaaatc gccttgtcta
    16081 tgaccactga tgatatttta gacgtttttg ctgaggcaga aatctatcca tcaaaaggcg
    16141 aaatcggggc atttttgaga aaagaaggcc aacgaaattt taagccttgc ggagataaat
    16201 atatgcgtaa ttttctcaag gggttaggaa tttataatcg gagaaaagta taaagaaatt
    16261 tcatgagaaa tcaaaacaat ggttgttttg atgagagcta gtttttgtat gagattaaaa
    16321 tattatctta atcgaggagc tggattggtt ttatgaatga tgaatataag aatgatgaag
    16381 ataagatgct ttttgaagag atagagaatc gatgtaggct caattttgag cttcggggaa
    16441 agatgtcttt aatacaacaa aaaaagtatt tagcgaataa atcagagttt actctgggac
    16501 atgttgaaaa attaatctcg gattggatta gtagtagaag cgaatttaca aaaataaaac
    16561 agccgattaa atttgacatg aaaaaacttt tgttaaataa gagtgagata ggtaatagag
    16621 atcaatatat tagagctaaa gggcaagaaa taattgattc attaggtgaa atgaggtcat
    16681 acaattattt gtatgtaaca catagagctg atggaatggt cattactgtg gggaaatctt
    16741 cttctaatga tattttttta gatggagatc ttttttatca actcaataca aatcacttgt
    16801 ctggcactga aaatataatt ttaaggacgg aatatggcaa tgaaatattt gcaaaatatg
    16861 atgagatttt aaaaaattat ttggactggg cttggataat tccggttgag tcaggagatg
    16921 ctaagaaact ggagagatta ttgggcgatg aactaattaa taaaaaagta ccaattttaa
    16981 attattattc ccatagacaa taaaaggcta tttttcatga taatactagt tataactgta
    17041 aaaatggaag aaaatctggg aaagaagttt ttaaataagt gtattgttcg attttaataa
    17101 ataataagca ataccatccg tctgtgttgc gcaacatcgt actacaatga atattttttc
    17161 acaaaaagaa aatcatccat cgaatgaatg gaaggtttta gtatacacga ggttaatgcc
    17221 ccctctttta taatcagagg agtgaggttt tttctatgaa tgtttctatt ttcttattgc
    17281 tagtctagtt tctcttacga tgaaattggg attctattcc agtttttatt gatttgctga
    17341 taaaataact tgaatggtgg atataaacat gataaataag agaagaataa caaaacaagg
    17401 ttgtgaaatg attctcaaat gaatataata gaaagaaaaa ggaggaagaa tcaatgaaaa
    17461 tagcaattgc aggcgctggc gcaatgggaa gtcgctttgg gttaatgctt caccaaagtg
    17521 ggaacgaagt attattaatc gatggctggg cagaacatgt ccaacaaatc aaagagcatg
    17581 gattacaagc aaattttaat ggtaaagagg tagaagcaaa actaccaatc gttcttcaat
    17641 ccgaagtaga aaaagaagat caagttgatc tgattattct atttaccaaa gcgatgcagc
    17701 tggaaaaaat gctgcaggat atccaatcat taatcaaaaa agatacagag gtcttatgtc
    17761 tattaaatgg tatcgggcat gaagatatta ttgagaaatt tgtaccaatg gaaaatatct
    17821 atattggaaa taccatgtgg acggcaggtc ttgaaggtcc tggtcaggtc aaattatttg
    17881 gaagtggttc agtagaatta caaaatttag gtgatggaaa agaagcagct gcaaaaaaat
    17941 tagcagacaa attgtctgaa tcaggattaa atgctcattt ttctgacaat attcactatt
    18001 ctatttatcg caaagcatgt gtgaacggaa caatgaacgg actatgcaca atcttagacg
    18061 ttaatatggc tgagctagga aaaacatcga ctgctcataa aatggtggcg acgattgtca
    18121 atgagtttgc caaagtagca gcagtagaga agattgaact agatgtccca gaagtcattg
    18181 cacattgtga atcttgtttt gacccggaaa caattgggtt acattatcct tcaatgtatc
    18241 aagacttgat taagaaccat cgattaacag aaatcgatta tatcaatggc gcaatttcta
    18301 gaaaagggaa gaaatatggt gttgcgacgc cttattgtga tttcttgact gagcttgtcc
    18361 atgcaaaaga agatagtttg aacgtaaaat aaaggagaga ttgaatggaa acttcacaaa
    18421 aaatatcacc gaaaattttt ctgaataaag tcctcgctgg tacagcaaca ggaatcatta
    18481 ttggattgat tcctaatgca gtattaggtg ccattttaaa atattttagt gcgtatccct
    18541 ttgcggtaac gatcgcacag ttagcagtta tttttcaact agcaacacct ttgatcattg
    18601 gtggtttgat tgccttacaa tttggatttg atccgatgag aatgatggtt gtagccggag
    18661 cttcttttgt tggttctggt gtagtcaaat tcacacctga tttgggtgaa acgggtgttt
    18721 acgttggtgc tggtacaggc gatattatta atacgatgct gacagcatcg attgctgtcg
    18781 ggctgatttt actgattgga aatagttttg ggtcagtgtc gattgtgtta acaccgattg
    18841 ttgtaggtgt aggtgcaggt ttacttggtt tttatttatt cccttttgta acagcaatta
    18901 cttctgcaat tggatcattg attaataact tcactacctt acaaccgctg ttaatgtcta
    18961 tcttgattgg ttgttccttt gcctttttga ttatctctcc tatttcaaca gtagcaatcg
    19021 gaatggcaat ccaactagat ggtgtttctg ctggtgcggc tgcgatgggc gtagctgcaa
    19081 ccacttttgt tttagtagtg aactcttgga aggttaataa gtcaggtgtg acaattgcga
    19141 ttgctttagg cgctatgaag atgatgatgc ctaatctatt tagaaaacct gtgattctta
    19201 ttccttgttt agttagtgca gtgattacag ctattcctgt cgctctattt tctatttcag
    19261 gtacgcctgc ttctgctggt ttcggcgtgg tcggacttgt tggtccgttg gcttctttag
    19321 atgcagggct aaatggcttt cttgttgttc tttgttggtt agtcatccct gtttttgcgg
    19381 cactaattac tcaattttta tgcgagaaag tatttaaact gtatgatcga gaagaagtct
    19441 tcaaattttt aggctagtag attttactaa gaaattttca atcaatggat gcgtaagtga
    19501 gatgtcaaca acatctgact tacgtttttt tgcctttttg agtaggagat tgatgctgac
    19561 aactgaactc ttcagtgcca ttcgattttt taaaatgtag aagaaactca cctttatgtt
    19621 attgattgtt caaaaggcaa ctagaataaa aatatgtgtt aaaatgagaa aaaatttgat
    19681 tgttggatgt atcgaataat cactaaaaag taggagaaga aaatgatttt tttgcaaaaa
    19741 tatggttttt attttttatt attaggtgtg attagtgatt tatcgacacc gtatatcctt
    19801 ggcctttttt atcccaaact gaatcaaatg actaccgtaa ttagtgtgtt tggtgacgta
    19861 gatagtccag tcagacgtgc tttcttggtc tggtcagttg tatctggact tttttttgtt
    19921 ctttcgttgc ctgcgttgta tcacctattt attggaacat caaaaacact tgcaatactt
    19981 gctgttgcaa cagttggttt atatgggata ggtgattgta tttttactgg gttattcagt
    20041 atcaatacga atgaaagctc ttggaatcta tcgacttgga tccataatat aggttctggg
    20101 ctaggctatg caggctttct acttttccca ttgttacttg ttctgcttta tcgtcaaagt
    20161 ggtcaaggta cgcttagcca tttttatctt gttttaacag ttattagttt actaattgca
    20221 ggtctttacg gattagcacg gattccgtcg attagtcagt ttgccttctt taagcaattg
    20281 ggcttctttc aacgcttaag ttttttcttt aactatttgc cgatgatttg tttaagtttt
    20341 tttcaactac gaaagtagaa aaagcagtgt gaagctgatt aagtaaaaga atttctctat
    20401 gggtaaccat tcgtgtggat gtgttcgact aagaatagag atgaggagag cgaaaagcaa
    20461 gttagtctta acttgttatg cgttgtgcta aatttcaggt agtcattttt tgaatgaata
    20521 agaagaaaac ttgaaagagg gtgacttcat gttagatgtg gagcagatgg aaatagacct
    20581 ttataatatt attttaaatc gaaatacaag agaatgggaa agaacacaat ttgaagaggc
    20641 aaaagaagac ctagcaaaaa tgtcgataaa gcaggtgata gaaaaactag aggtcaactt
    20701 gcgtccgcta gcactacgag ataatttgac accagatgcg atggattttt atttaagaat
    20761 cacaggggag tcagcgagtg caaaggctta tgattatgcc aaacataaga taacttcacc
    20821 cttataccaa gaacgggcga tttttgccgg tggctgtttc tggtgtatgg ttgagccttt
    20881 tgaaacaaaa ccaggtgtaa tctcagtgtt atctggttat acaggtggag agaccaaaaa
    20941 accaaattac gatcaagtca gtggtggata tactgggcat gttgaagctg ttgagatcat
    21001 atttgataca cgtcaaataa cctatgaaga gctgttggag atttattggc aaatttcaga
    21061 ccctacagat gcctttggtc aatttcaaga tcgggggaaa caatatcgtt caattctatt
    21121 tgttgttaat gaaaagcaaa ggcaattggc tgaaaaatca agagaacaac tgattcaatc
    21181 taaccgattt agccgtccta tcgttacaga gattcgccaa gcaaagactt tttggccagc
    21241 tgaaaatcat catcaacaat tttatcagaa gcaacctaaa agatataaaa agatcaagca
    21301 ttcgcatcag cttcatttat tttttcagcg acatagatag aggaaggttg tggcgcaaag
    21361 cgttcagctc cgacaaaata atgagcaact gacaaaaata gcttttcata tttttggaca
    21421 gttatgaatt atttcgagga gttgctttga gaacaccgag gaaaaggttg tggcgcaaag
    21481 cattcagctt caagtcgcaa ggaaacagga ggtatgacaa gtcttttgtc acaactcctg
    21541 tttttttact agcctttatt tccatgtgaa agagaattcg aattgtagaa tcttgatttt
    21601 tctatggaat tttgtagaga atcatcttga taattaatag acacctaatc gaattccaca
    21661 ttaatgatga actaaattcc gtataaaagc gcaaacaata actata
//
LOCUS       DAYYAR010000042        21302 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000042 DAYYAR010000000
VERSION     DAYYAR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.042"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHAT0_001319"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHAT0_001319"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8451269.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHAT0_001320"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHAT0_001320"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8451270.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHAT0_001321"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHAT0_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8451271.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHAT0_001322"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHAT0_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8451272.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHAT0_001323"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHAT0_001323"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8451273.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHAT0_001324"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHAT0_001324"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8451274.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACHAT0_001325"
     CDS             complement(8053..8523)
                     /locus_tag="ACHAT0_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8451275.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACHAT0_001326"
     CDS             complement(8569..8994)
                     /locus_tag="ACHAT0_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8451276.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACHAT0_001327"
     CDS             complement(9061..9330)
                     /locus_tag="ACHAT0_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8451277.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACHAT0_001328"
     CDS             complement(9341..10936)
                     /locus_tag="ACHAT0_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8451278.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHAT0_001329"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHAT0_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8451279.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHAT0_001330"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHAT0_001330"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8451280.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACHAT0_001331"
     CDS             15402..16376
                     /locus_tag="ACHAT0_001331"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8451281.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACHAT0_001332"
     CDS             16546..17946
                     /locus_tag="ACHAT0_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8451282.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACHAT0_001333"
     CDS             complement(18024..18746)
                     /locus_tag="ACHAT0_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8451283.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACHAT0_001334"
     CDS             complement(18825..19331)
                     /locus_tag="ACHAT0_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8451284.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACHAT0_001335"
     CDS             19454..21253
                     /locus_tag="ACHAT0_001335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8451285.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLFQSSFETIAFRLNEICRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5925 a         4667 c         3840 g         6870 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttttca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt tgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ct
//
LOCUS       DAYYAR010000043        20264 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000043 DAYYAR010000000
VERSION     DAYYAR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.043"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..2166)
                     /gene="glyS"
                     /locus_tag="ACHAT0_001336"
     CDS             complement(91..2166)
                     /gene="glyS"
                     /locus_tag="ACHAT0_001336"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8451286.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2168..3085)
                     /gene="glyQ"
                     /locus_tag="ACHAT0_001337"
     CDS             complement(2168..3085)
                     /gene="glyQ"
                     /locus_tag="ACHAT0_001337"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8451287.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3469..4281)
                     /gene="recO"
                     /locus_tag="ACHAT0_001338"
     CDS             complement(3469..4281)
                     /gene="recO"
                     /locus_tag="ACHAT0_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8451288.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4424..5323)
                     /gene="era"
                     /locus_tag="ACHAT0_001339"
     CDS             complement(4424..5323)
                     /gene="era"
                     /locus_tag="ACHAT0_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8451289.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5338..5733)
                     /locus_tag="ACHAT0_001340"
     CDS             complement(5338..5733)
                     /locus_tag="ACHAT0_001340"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8451290.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5717..6193)
                     /gene="ybeY"
                     /locus_tag="ACHAT0_001341"
     CDS             complement(5717..6193)
                     /gene="ybeY"
                     /locus_tag="ACHAT0_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8451291.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6210..8402)
                     /locus_tag="ACHAT0_001342"
     CDS             complement(6210..8402)
                     /locus_tag="ACHAT0_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8451292.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8426..9397)
                     /locus_tag="ACHAT0_001343"
     CDS             complement(8426..9397)
                     /locus_tag="ACHAT0_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8451293.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10103..11482
                     /gene="brnQ"
                     /locus_tag="ACHAT0_001344"
     CDS             10103..11482
                     /gene="brnQ"
                     /locus_tag="ACHAT0_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8451294.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11638..12084)
                     /locus_tag="ACHAT0_001345"
     CDS             complement(11638..12084)
                     /locus_tag="ACHAT0_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8451295.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12111..12287)
                     /gene="rpsU"
                     /locus_tag="ACHAT0_001346"
     CDS             complement(12111..12287)
                     /gene="rpsU"
                     /locus_tag="ACHAT0_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8451296.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12449..12877)
                     /locus_tag="ACHAT0_001347"
     CDS             complement(12449..12877)
                     /locus_tag="ACHAT0_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8451297.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12969..13829
                     /locus_tag="ACHAT0_001348"
     CDS             12969..13829
                     /locus_tag="ACHAT0_001348"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8451298.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13913..14263)
                     /locus_tag="ACHAT0_001349"
     CDS             complement(13913..14263)
                     /locus_tag="ACHAT0_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8451299.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14279..16006)
                     /locus_tag="ACHAT0_001350"
     CDS             complement(14279..16006)
                     /locus_tag="ACHAT0_001350"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8451300.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16219..17145)
                     /gene="trxB"
                     /locus_tag="ACHAT0_001351"
     CDS             complement(16219..17145)
                     /gene="trxB"
                     /locus_tag="ACHAT0_001351"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8451301.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17290..18723)
                     /locus_tag="ACHAT0_001352"
     CDS             complement(17290..18723)
                     /locus_tag="ACHAT0_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8451302.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18723..20126)
                     /locus_tag="ACHAT0_001353"
     CDS             complement(18723..20126)
                     /locus_tag="ACHAT0_001353"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8451303.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
BASE COUNT         5466 a         4402 c         3618 g         6778 t
ORIGIN      
        1 aattaataat agttatttat atttataaca aataaaccag agaaatagcc tattttgtgc
       61 ctgttctctg gtttatttac atattagaaa ttattttgtg atgatttgat ctaaacttgc
      121 tagtgacaaa gccatggcag caatttgact taattgcgtt aatcggttct tacgtactgc
      181 tggttcttct gccatgacca tcgtctgatc aaaataattt tcgatcagtg ggcgaaggct
      241 tacaagctga tcataatttt cgttgatcgt ttggtcagca aagcttgttt tcattgcctc
      301 tactgctttg tgcagttctt tctccgcttc gttttcgaac agttcagggt ctacttctga
      361 ttgtttttca agctccgctt ttttcgctaa gttcacgaca cgtgtcaaag cttccatcga
      421 tggtttgaag tcttcatctg caagatgctg cttgaacaat tgtgctgctg caaatacttt
      481 tgtcagatct ttttgttctg cattcaaaac agcttcgatg acatcatgac ggatattctt
      541 cgttgtcaat aattggcgca gacggccttt gataaagtcg atcacctgtt gttgtcccgc
      601 tgacaagcca atgccaaaac gatccgtatc tttactgata acttcactaa tcgtttcttg
      661 cagaacagac aaaggaaagg cccatttttt cgattcgatg attcgaacga caccatatgc
      721 ttgacggcgc aaagcatatg gatcgtttga gccacttggg atcatcccaa ctgcaaagaa
      781 agaaaagata ctatctaatt tatcagcaat cgctaaaaca gaacctactg tagtctcagg
      841 taaatctcct tcactggata taggcatgta atgttcacgg atcgctgttg ctacggctgg
      901 tttctcgcct ttcagcagag catatttttc tcccatgatc ccttgtagtt cagggaattc
      961 tcctaccatg ttggttacta gatcaaattt atagatttca gcagcgcgtt gcagatccat
     1021 caattcttcc tcagatagtc ctacttgttc gcctatgatg cgagcgatga cttgtacccg
     1081 ttgcattttc tcatagatgc taccgatttt ttcatggaaa gtaacagttt ttaatttttc
     1141 aacgtattgg tcgattgaag ctttcttgtc ttcattataa aagaattccg catcttctaa
     1201 acgagccgtc aatacttttt gattgcctcg taccacattt tccaatttta cccgatcgcc
     1261 attacgagca gaaataaaat gaggaagcaa cataccttga tcgttccgta cttcgaaata
     1321 acgttgatgt tctttcatag aagtgactag tacttcttca ggaacagata gatatttttc
     1381 ttcgaagtcg ccagaaaaga cagttggata ttcgactaaa ttgttgactt cttctagtaa
     1441 atcttcatct agatcaatga tccagtgatt tttttcggct aattcgtttg cttgtgtgat
     1501 gatcatttct tttctttttt gcggtgaagc aataacaaat tgttcattca atttttcttc
     1561 gtattctttt gcatgcgtaa atgtcacatc ttcgcctaag aaacgatgac ctcttgaagc
     1621 tctgtctgtt ttgacatcca agatctcaaa cgggattatt tcgtcatcta acaatgccac
     1681 gatccagtga atagggcgaa tatattcaaa atcgtattct gcccagtgca tcgttactgg
     1741 gaaagtgagg cttttaatga catctgctaa acctgtcaat acttcttttg cttctttgcc
     1801 gtgagtgtat ttcgttacat agacatattc cacgcctttt aattctctaa aagtgatagc
     1861 atctgtcgtc agaccttgtc cgcggacaaa tccttcagct gctttcgtcc agtttccatc
     1921 tgcgtccaaa gcaatttttt tggctggtcc tttgacttct tcttcgctgt ctgcttgacg
     1981 ttctggaatg tttgttactt tgattgctaa gcggcgcggt gtagaaaacg tttcgatcgt
     2041 atcataagtc aattggtttt catctaaaaa ttttgctgtt ttttcttcta attgtttcat
     2101 gcttggtgtg acaacatgag caggcatttc ttccagcccg atttcaagta ataagtcttt
     2161 tgccatctta ttcagcctcc ttcatgtttt ctggatttgc tgtgttttta ttcaataaag
     2221 ggaatcctaa tttttcgcgt tctgccacaa agattttggc tacagcacga gccatgttgc
     2281 ggatacggga aagataacct gctcgttctg ttacggatac tgcaccgcga gcatctaata
     2341 aattgaatgt atggctgcat ttcaaaatat aatcatacgc tggatgaaca agattttcat
     2401 cgatacagcg tttagcttct ttttcaaaac ggtcaaagtt gcttagtagc aagtcttggt
     2461 cacttacttc aaaagaatat ttggaatgtt catattctgg ttgtttgaag atctcgccat
     2521 atttcacacc atcagcccat tccagatcat agacactttc aacttcttga atatatgaag
     2581 caagacgttc tagtccataa gtgatctcag aagtaactgg tttacaaggt agtcctccga
     2641 cttgctggaa gtacgtgaat tgtgtgatct ccatcccatc aagccatact tcccaaccca
     2701 caccggcaca ccccatcgat gggttctccc agttgtcttc aacaaaacga atatcgtgtt
     2761 ctaatggatc aattcctaaa agacgcaagc tttctaaata aagctcttgg atattttcag
     2821 gtgaaggttt cattacgact tgaaattgat gatgctgata caaacggttt gggttttctc
     2881 cataacgccc gtcagctggt cggcgggatg gctctacata agcagcattc catggttccg
     2941 gtccgattgc ccgtaagaat gtatatgggc tcatcgtacc tgctcctttt tctgtgtcat
     3001 aagcttgcat cagcatgcag ccgtttgatg accaaaattt ttgtaatgtt aaaatcatat
     3061 cttggacggt caatttttta ctcatctgtt ttgccccttt caatttttaa gcgttgctgt
     3121 caataagaat acaggaaaga aaacgacaaa aagtccctat gtctgattac agacataggg
     3181 acgaataatt tcgcggttcc accctacttc cgaataaaga ttcggcagct tcatttatcg
     3241 tactcgcaag tgcctcttca aaaatcgttt gggtccggct tccactctcc cgaactcgct
     3301 aaaccacgtg ttttttactg atcttggtca atgtacgtat tcattttgca gttttcttgt
     3361 ttatataata gatcctctca gtgaacgatc tatttaatct acggttaata atagacggaa
     3421 gtccctgtat ttgtcaaata gttttgattt ttttgttgta tctttcattt attcttcttt
     3481 ttcaggtatc tctttttcag gcatattttt ctctggcgtc tttaatacgt gtgcccaaga
     3541 cttcatttga tcaatgaact ttttgctttt tagatgaata ccgacatatt catcatacaa
     3601 ctggtcaatg aggtcacgga ttgcctgctt cgtttcttct ttcaagtgga ttccactgat
     3661 cttatcataa gaaatagccg aaaacattcg aacgaaataa acagcgcgag gatctgcatg
     3721 ataacggtgt tcgtccattg gccagtgttt ctcgcatatc acaccgccag atttggaaga
     3781 atagtcaaat tttccttttg tttccccgca tacgacacag tgcgtccaaa ttggcgcgat
     3841 tccgaagcgt tgcagtattt gaacttcaaa gatattcgtg atgatttctg catcatctcc
     3901 ttgatccatc cgctgcagag cttgttgcgt aaattggaat aaatgcgggt catattgatg
     3961 atcttcgatt gctgcatcta ctagatttag caaataggta ccataaccgc tcaaaaagat
     4021 atcttcttgg atgttccgaa acgcatggac gtctttgaca ctgcttaaaa acgacaaacc
     4081 ttcacttcga aaattaccga tatagactgc ttcggtaaag gggagcaaag ccggagctaa
     4141 tggctgattt tgccgatgga ttcctttgat ataaaacatc attttgccag aagattccgt
     4201 aaagattttg actaatttat ctttttcttt gtaattacgg ctagataaaa tcaagccttt
     4261 cgattctgca agctggctca tattctccct cctaaaaaat aaatctggaa aagcagattc
     4321 tgccgatcca gattatttat gtttcgatct tccgccgttt ttctcaacgt gcggattttt
     4381 atccagttta tttctatact taacctataa gaagttcaga atatcagtat tcgtcttttc
     4441 ggtagccaaa gtcttgcaga tagacttttt tgtctcgcca gtctttttgg actttgaccc
     4501 aaagttctag aaatactttg tctcctaaaa gattttcgat atcctttctt gctttcgttc
     4561 ctacttgctt cagcattttt ccgcctttgc cgataatgat tcctttttgg ctatctcttt
     4621 cgacgatgat cgtggcctgg atatggattt tatcattctc gtttcgcttc atcgaatcaa
     4681 caacgactgc gacagaatga ggcacttcgt ctcgtgttaa aaacaagact ttctcacgga
     4741 tcaattcaga aacgataaag tattcaggat gatcagtcac ttgatcatct ggaaaatatt
     4801 gtgggccctc tggcatttca ctgactaata catccattaa gcgttcgaca ttgtttcctt
     4861 ctgtagcaga gatcggaatc acttgtgcaa aaggcatctg cgtcgaataa tcttcgatga
     4921 ttcccagcaa ctcgtctgga tggatcttat cgattttatt gataatcaga taaactggag
     4981 aatgcacgtt ttttagacgt tcaataataa aatcgtctcc ccggccgcgt ttttgatcag
     5041 cactgatcat aaataagact gcatccactt ctttcaacgc actgtaagct gcttctacca
     5101 taaaatctcc tagacgatgc tttggtttat ggattcctgg tgtatcgata aaaatcagct
     5161 gagcttctgg tgtagtatat actccttgga ttttatttct agttgtctgt gccttgtcac
     5221 tcataatggc aattttttga cccacgattc ggttcaataa tgtggatttt cctacattcg
     5281 ggcggccgac gattgcgaca aaccctgatt tatgttctgg catatacgtt ctccttttta
     5341 attaaacaac aaatgatata ttttagggac aaataaaatc aacccaatga tgacagcaaa
     5401 acaagcggtc actaaaactg ctccagctgc catatctttg atttttttcc caatcggatg
     5461 aaaatgaaaa tccgtaaaca tgtccactac attttcaaaa acagtattga tgatctctac
     5521 tatccaaacg agaaaaacag ctaaaagcag ccataaccac tctatccgat caagctggaa
     5581 aatcacgccc ataaataaag caaggatacc gaacgcaaca tgtttcctca tattgcgttc
     5641 ttcctcaaaa acggttttga ctccttggac agcaaattcc acagacatca ggaaatgttt
     5701 gtttttctct acgtttttat cttttgagtc cataggcatc caagatctct ttctgtaaac
     5761 caaacatttc tttctcatct tctggtgtca tatggtcata cccgttgata tgcaagaatc
     5821 catgtaccgc taaaaagcca agttcccgat catatgaatg gccgtaagct tctgcttgtt
     5881 cagcagcacg ttcaatagaa atcatgatgt ctcctagtac acgaggcaaa ggattctctc
     5941 catcttcaaa aatcactggg atttcgtctt cgccttcttc ttccagagca aaactgatga
     6001 cgtctgtcgg tgcatctttt ccacggtaat cacgattgat tacttggatt gccgcattgt
     6061 ccatgaaagt aacagacatt tctgtttcct ctggcagctt taaataagat gctgcaaatt
     6121 gcagcaagtc atcgatttct ttcattttct cttctgagac ttggtttgtt tcatccataa
     6181 aggtaatatc cattatacat cgcttctttc tattcttctt gttcttcctt tatcttctca
     6241 acttctgttt tcattttagg atatttgatc cgagaatgga aggtgctact taaaccgctg
     6301 atcaatgatt tttccacttt tcggatttct ttcaaggtca gtccactatc gtctaattga
     6361 ccatcggaaa tacgctcttc gataagatta tggacaaaac tttcgatttt ttcgctcgtc
     6421 ggatgatcca ttgcccgtac tgcagcctca caactgtcag caatattgac gatccctgct
     6481 tcacgacttt gcggttttgg tccaggataa cggaattgct cttctgttac ttccggattg
     6541 cgttctttcg ctttgatgta gaaataacgc attagcgtgg ttccatgatg ctgtttgcaa
     6601 atatcgatca ccatttgcgg catattataa tcttccaata ttttggctcc atctgtcaca
     6661 tgtccaaaga taatatgttt actgtcttct ggcaataaga aattgtgcgg gttttccgct
     6721 cctgaaggca agttctcaac gaaaaagcta gcatgtttga tcttgccgat atcatgataa
     6781 tagcaagcta ctcgagttag caaagaacgt ccgccgattt ctgctacagc gttagcactt
     6841 agattagcta ccatcatact atgatgataa gtacctggcg cttcttccag caactgtttc
     6901 aataaaggat gatttggatt gctgagttca tttaatacaa ttacactgtc atcattgacc
     6961 atcaattcaa tatatgggtg tagaccgatc gtcagcaaga atgagaagag tgcaccagtg
     7021 aacccgcaga caagcattaa ccaagtcgtt gcatcaccaa agctcattcc ttgatagacg
     7081 atcaaaatga catccatcat cacaggaaaa ataatgaccc acatggcagc agataatcct
     7141 tgttctgaga tccgtttgcg tctgacaatc gttgccatga gcccagagaa aacataagac
     7201 atcaagataa tcgttagaaa gttcgtgcca atcgaatcat aaaaaatgaa aagtgcagat
     7261 actgcttgga aaataccagc catgatccct gctcttcggt tcaagaaaaa gcttaaaaca
     7321 agtggtgcaa aagcagcggg ataaaacagc gggatataag tagcttgttc cgtttggaat
     7381 aattggaaaa atttcatcag cactatgctg actgacatag ctgtcacata gaaaagcacg
     7441 tattttgttc tttctcctgc attttggaat tgaagtgaat tataaatcag tacgccgatt
     7501 tgaagcaaaa cagctaaaac catggcaatg agtgggaaaa tcgaagtcgt ttgattcgtc
     7561 aatcctagta attccaattt tttcatcgct gaagcatcaa tttgatttcc ttcgcgaaca
     7621 atgatttctc cttggaagat catcactggt tgtacagctt ctcgtgcaga ttgttttaat
     7681 tcttctgtct ttttctcatt caatacatca ttgactacaa tgccttgatc cactaagtag
     7741 cgaatcattt gttgctgctc tgttgtgaca ttcaaatact gaatctgatc ttctgcttct
     7801 tgtttaaaag cttccaattc gctttcacga acttgtttac tcatctgttc gtcaatgagc
     7861 ttcaaacttt catccctaac cgtgtgaatt tccgtttcag tcatttcaaa tatcgtcgaa
     7921 taaaaattgt tcggcagttt ttggtaaaac gcaacgtttt ctgcattcac gttttcaaaa
     7981 tttttcttca gtgtagcaat ccgctcttct gtagtaggtt tcgggatatt ttcatcttcc
     8041 ttcgccttat ctaccttgtc attataagat ttgttgatgg tatcattcgt cttatcgatc
     8101 aattcgaata attgcttgat ccgattgttc tggtcatctg ccagatcttt ttgataagta
     8161 tattctggag tcactgcttc tgctgccagt tttcttcttt gttcggtttc ttccgtgttc
     8221 tcgatcgttt tattcgcacg gatactctct tctgctactt ggccttctct gaaatcattg
     8281 gccttttgtt tgacactact aaatgtaacg ccgcagacaa tgataaaaaa caagaacaga
     8341 aggaaaggca atgctgcctt tccgagtttt tcttgtagtt tctgcagtgt tttattcaac
     8401 atgtgcttat cctcctaagc aaagcttatt cttgatgaag atccgccttt tcataagctt
     8461 tgatgatttc ggcaactaca gggtgccgaa caacgtcact tgcttcaaaa ttaacaaaat
     8521 cgattttttt gatttgcttt agtgttcgtt ctgcatgaac aagaccactt gtcgtccctc
     8581 gaggcaagtc tatctgactt gtatccccat tgacaatcat ttttgattga taaccgagac
     8641 gggtcaagaa cattttcatc tgagcgactg tcgtgttttg tgcttcgtcc aagataacga
     8701 atgcatcatc caatgtccgc ccccgcatgt aagctaatgg tgcgatttcg attacgcctc
     8761 gttccatcaa tcggttcgta tgatccaatc caaaaatttg gtataaagca tcataaacag
     8821 gccgcagata aggatccact ttttctttca gatccccggg tagaaagcct aggttttccc
     8881 ctgcctctac agcgggccgc gtcaaaatga ttttttgcac ttcgcctttt tttagggcag
     8941 caattgctaa tactacggca agaaacgttt tccctgttcc ggctggccct acaccaaata
     9001 cgatgtcatg tttggctacc gattcaacgt atttcttttg accgctgttt tttacccgta
     9061 taggttttcc attacggtct ttgacgatct cttcctcata catctcaaca aaataatcta
     9121 atgtaccttt ttgccccatt ttcaatgccg tcacgacatc tggtgtactt acgtggattc
     9181 ctcgtttgat caattcctgt aatgtccgaa tgactgaggc agccaaggaa atctgggatt
     9241 gttcaccaat gatctgaatg atttctccac gtgtatggat ggttgtttcc gttgtttcct
     9301 cgatcagttt tatatgcttg tcgtgtgcac ctaaaagcat gctgacatca tcagcagctg
     9361 tcaatctaat ctctaaagaa ctagactctt ccgtcaaaaa tagacatccc ctttttttcg
     9421 attcattctt tcctatttta ccatactcag aagaaactat atagcaaata agaaaaggtt
     9481 gtaaaagagt cgtttaacca tacgtaccaa gaaacgttaa aaaatagctg tttccattcg
     9541 ctaaaggtct cgttttgata aaaagaatct gagaatgtaa gttatatgaa agtggagaac
     9601 aaaatattcc gtttcattca gaggtattct ctaggtattt ttcttgtttc aaaaaaagaa
     9661 aacaaaatag ttaaaatatt cttgcatttc aaaaaaaaca ggtgtattat tacgtcaaca
     9721 aataaacata cagcaaagcg tcgaggaaaa cagtaggcta ttcaacgatt tattcccagt
     9781 gagtcatgta agtgagaaat gacaataaat cgaatagacg aacatcattt ccgagtcaat
     9841 gtcgaaaacg tagatcattg tggctgcctc cattatcagg ctagcggata actttatcaa
     9901 aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa ggtggtaccg ttctagaatc
     9961 tagagccctt ttcccaagtg gaaaagggct tttttttata ttttaaggtg gtggttttca
    10021 tggaatctga tattagtggc attactaatt gctgcaacgt ttatatcgtc aatcaaaatg
    10081 ataattattg gaggaaaaat ttatggaaga aacaaaaact tattcatgga aacaactatt
    10141 gatggtcagt tcacttatct tcgggatgtt tttcgggtca ggtaatctaa tctttcctgt
    10201 ccacttaggt caaatggctg gcgcaaactg ggtcagtgct ggcgtaggat ttgccatctc
    10261 aggcgctctt ttcccattac tagctatctt agctgtagta gtgacaaaaa gtgatggtct
    10321 gtatgactta gctaaacctg tcggaaaatg gtatgctgcc ttgttcttaa tccttgttca
    10381 tttgacgatc ggaccatttt tcggtacacc acggacagct gctactgctt ttgaaatggc
    10441 tgctaaacca tttttgcctg aaaaatataa ttcattaggt atgtttttat tctctgctct
    10501 tttcttttta cttgcttatt tccttactgt ccatcccaca cggttggtca aatacgtcgg
    10561 aaagtatcta aactccttgt ttcttgcatt attagcaatc atttttgtcg tttctttatt
    10621 ccacccaatg ggaaatctaa atcaatctgc tgcagatact taccaaacaa acgctttaac
    10681 aaatggtgtc ctagaaggtt ataacacggt tgatgcagta gccttgcttg ccttaagtgt
    10741 cacttttgtc catgcagtcc gtgaccttgg tttcaaagat aaaaaagtca cagaattaac
    10801 agcaaaagct ggtagcctga gcgttgcgct agaagtcatg atctattttg gtcttgttct
    10861 tctcggtgcg atgagtttga ataagatgtc tttatcagaa aatggcggaa ctgcactttc
    10921 tcagatcact aatttttatt taggtaactt cggcgctagc ttcttaggag tgatggtcac
    10981 attaggtgtc ttcactaccg ctatgggact cgttgtttct tttgcacaag atttccataa
    11041 actgttccct aaagtcagtt atatgacatg gctgcggtta acgactttcg tctcttttgt
    11101 cgttgcaaat gctggtttgg acaatatcat ccaatggtct ttaccagtat tgatgctttt
    11161 gtatccattg tcgcttgcgt tgattttgct ttccttaaca gcaaaatttt tccaaaagac
    11221 accatttgtc tatcaagtaa cgatgttgtt tgctgctgtt cccgccgtat tggatatgct
    11281 ggcaaattcg cctgctttag tgagtcaaca aagagttgta gcaagcatgc tcgaatttta
    11341 tcatcatcac gttccttttg ctgcattggg acttggctgg atggtcccaa cgttacttgg
    11401 gtatgctggc agcttactct tctattacgc ctatcgccta tcagggtata aacaagaagc
    11461 aaatgaacta ccagaagaat aaccagatca aacacaattt aacataagaa atattaaaca
    11521 ggaaaaaaac agctagatga aagaatccta ttctttcgtc tagctatttt ttagatctct
    11581 tttttacgag cacaaaaaaa ctgcggacaa tgattgtccg cagtttaaat gataaaatta
    11641 agattgtaat agttctttca caatggcatt tacttggttt ccatctgctt ttcctttgac
    11701 tttaggcatg atcgcaccca tgactttgcc aaattctttc attgatgaag caccagtttg
    11761 cgtgattgct tcttgaacaa gttgtcgaat ttcttcgtcg gaaagttgtt ccggcatgta
    11821 tttttcgact atcacaattt cactttttac tttttcagct aaatcgtcac gtcctgcttc
    11881 ttcaaactca tgcaaagaat cacgtcgctg tttcatttcg cgagacaaca ccgttaattc
    11941 ttcttcttct gtcaagtcgt gaccttcttt aatctgctcg ttttgtatag atgacttgat
    12001 cattcggatg acttgtaaag attccttatc tttcgctttc atcgctgttt tcatgtcatc
    12061 gtttaatcta cttagtagtg acacactacc aactccaatt catcacagaa ctagaatttt
    12121 ttgcgttttc tagctgcttc agattttttc ttgcgtctta cacttggttt ttcgtagaat
    12181 tcacgtttac gagattcttg taaagtaccc gcttttgaca cggaacgttt gaagcgacga
    12241 agagcatcgt caagtgattc gtttttgcga acgactgttt ttgacatgtt aattccctcc
    12301 ctccgagctt aaaaagtaac cgtaattgtt attgaaatat atctcaaaaa acaagactgt
    12361 cctgtaaata ttatatctaa tgctgttttg accgtcaagg aaatcttgct tttttttaaa
    12421 attatcaaat gttattttat ttaaatcctt attgacaatt ttcacatctt ccgaaaatct
    12481 cgaatctatg cccttcgatc gtacatccac cgagctgttc ttcgaaataa ttcattggac
    12541 acatatggat ttctttcgtt gttccgcaca ccgtacagat aaagtgatgg tgatggccga
    12601 tttccccttg acagcaatga aaacgaaact tcatctctcc atttaaatcg gtttcttcca
    12661 gtaaatcgat ttctgcaaaa tcatggagat ttcgataaat cgtatcgtaa cttactcctt
    12721 tgaattcctg attcatatag tcaaaaactt ctttagcgga tacgtagcga ttgcgcttca
    12781 ctaaataagt taacaatgct tctctttttt tagtatattt aaaaccgtta tttttcaatg
    12841 tttctagtga ttgttcgatc aacgtctgtt ttttcatgac ttattcctcc aaatcaaaat
    12901 gattacgaag tacacattct agagtatggt ataataaaca taaaaaaaat gcaagtgagg
    12961 ttttatccat gtcttccgat tctacagaaa tcgttacttt ttttgtcatc tccgactctg
    13021 ctggagaaac tgcgacaaaa ctagcgcaag ccactatggc ccaatatcct agcgttgagt
    13081 tcaatttatt ccgcagaaca tttgtgacag atgagaaaac attgatgcag gcactgcaag
    13141 atgcactaga agaaaaagct cttgtcctgc acacattgat caatcaagaa ttgatcgaga
    13201 tgacgcgttc tttttgtgaa gaacatcaat tatttagttt tgatgtgatg actccgccga
    13261 tatcagaaat cgagcgctta actggtatca agcccattcg gcaacctggc gcccttcatt
    13321 tattgaatga gaactatttc aaacggatca aagcaatgga atttgctgta aaatacgatg
    13381 acgggaaaga tcctcgtggg tttctagaag cagatgtcgt tttgctaggc gtatcccgaa
    13441 cttccaaaac gcctttgagc ttgtttttag ctaacaaaaa tttaaaagtt gccaatctac
    13501 ctttgatccc acaagcacat attcccaaac agctttggga gatcgatcct aaaaaaatcg
    13561 tgggactgac gaataaccca gatattttaa acaatatccg taaagaacgg atgcgctctt
    13621 acggattaaa tcctgatact gcttattccg atatcgaaaa aatacgagct gaactcgatt
    13681 ttgcgaatga tctttatgaa aaattgggct gcgtagtaat caatgtcgct tctttatcta
    13741 ttgaagaaac agcttcgctt attttgaatg ctttagacct agaagaccat agttattatg
    13801 gaactgaaac caccgaagaa acagactaac aatttttctt taaaaaaact ggactgtgtc
    13861 agcgaagaag attcgttgac acagtccagt ttgtctctat ttgcacctat cgttattgat
    13921 gttccacatg tttgagtgtc tggttcaaaa tatccagtac gtgagaatca tcaagactat
    13981 acaaaatcgc ttttccctca cgatgggact tgacgacatg gttttctctt aataaacgca
    14041 gttgatgcga tacagcagat tgttccattt ccactgcttg gatgattgct gtcacatttt
    14101 gttccccgtc tttcagatac agcagaattt tgagacgagt ggggtcactc aacattttga
    14161 atagttggct gactttctct acttttgttt tttgcgtgtc tggcaatcca ttcacctcca
    14221 acttgaaaaa agactgactt ctaaagttta ccactttttc catcagtctc aagttttttt
    14281 attcttctgt catcttttgg ataatcgttt cataagcggc tactttttca gctgcgtctt
    14341 ctgaagagtt tccttttaca ccgatataaa atttgatttt cggctcagtt cctgaaggac
    14401 ggatagcgat ccagccattt tcttccagcg tatacttcaa tacgtcagat ggcggtgtcg
    14461 ttagtttttc tacgtttcct tcacggtctg ttctcgtcaa ctctttgaag tcttctgttt
    14521 ggatgacttt tgctccggca aattctgttg gtgcttcttc acggaatttt tccatcaaag
    14581 cagagatttt cgcagaacct tcttgtccgc tcattgtcac agaaatcgtt ttttctgcgt
    14641 agtagccata ggtttcaaaa atcgtttgca agccatcata caacgttttg ccttgttttt
    14701 tatagtaagc tgctacttct gctaataaga gcaatgcttg gatcgcgtct ttgtcgcgaa
    14761 cgaaaggttt aactaaataa ccgtaacttt cttcgaatcc gaacataaaa gtatgggaat
    14821 gatcttcttc aaattgttga attttttcag caatgaattt gaatcctgtc aatacattga
    14881 acatggtagc cccatagtgt tccgcaattg ctgtaggtaa ttcgctagaa acgatcgatt
    14941 tcaataccgc tgcgttttca ggtaaagtgc ctcgcgattg gtgtgcttcc aaaatatatt
    15001 gagcaaacag tgctccgatt tggtttccgg tcaatacttc ataggagcca tttggcaaac
    15061 gaacggctgc acctaaacgg tctgcatcag gatctgttgc aatcagcaaa tcagcgcctt
    15121 ctttctcgcc aagtcgtatc gcatactcaa aagcagagtg ttcttctgga tttggtgatt
    15181 tgactgtagt gaaatctggg tctgcgacag cttgttctgg aactagtacg aattgttcga
    15241 atccagcttg tttcaacgca cgttcgccca acatttttcc tgttccgtga agtggtgtgt
    15301 acacaagttt caaatctttc cccattgttt ccaccaattc atgatcaatc gttactgctt
    15361 tgatttcttc tagataagcg gcatccactt cttctccgat gatcgtgatc aatccgctat
    15421 gttcagcttc ttcgtcagat aatacttcaa cttttaacgg attgctgact tcacggacat
    15481 atttcgtcaa tgcatctgcg tctgcaggag gcatttgtcc cccatcttct ccgtagactt
    15541 tatatccgtt gtaagctgct ggattgtggg atgcggttac catgattccc gcaaacgtct
    15601 tcaaatgacg gactgcaaaa gaaagctctg gtgttggacg tagactttcg aatacataag
    15661 atgggatatt gtgatgtgct aatgttttgg ctgcttccat ggcaaattct ggtgattgat
    15721 ggcgtgagtc ataagcgata gcgacacctc ggcgttttgt ttcttcgcct tgtgtatcca
    15781 tgaaacgtgc aagtccttct gttgcttgtc ttaccgtata gatgttcatt cgattgatcc
    15841 caggccctaa gattccccgc atgcctgcag taccgaattc caacggtgca taaaaagcat
    15901 cttcaagttt ttcaggatct tcacccagat catttaattg tttttttaag ttttcttcta
    15961 aattttcttc atgtacccat tgttcataca cttgttccca agacatgaaa ataacacctc
    16021 tttctgaaat atggttctta ataagtatag cattcactta ttagatatga aagtatgagg
    16081 accaaaaaac gcttaaatca agaaaggatt cttttatcaa gcacggttgc aagtattcgc
    16141 ataaatcgga ttcagtagat ttcacgcgaa aaaaaagagt aaaagaacaa ttaaaccgtt
    16201 cttccactct ttttaaactt atgcagttgt ttcttctaaa gattccaagt atttatacac
    16261 ttgttgtcca gcgatcccac catcaccaac agcagtagtg atttgtcgca aatctttttc
    16321 acgaacatct ccgatggcaa aaacgcctgg tatattcgtc ttcatttcgt tgtctgttgg
    16381 aatccagcca gcttcgttcg tcaaccctgc ttctctaaat ggtgcagtta atggatccag
    16441 tccaacatag atgaatactc cgtctgctgc caaatcaaac tcattgccgg ttttgacatt
    16501 acgtgctttc acacctgtca ctaccatttc attcccaacg atttcgtcaa cgactgtatc
    16561 ccacataaag gaaatttttt cattcgcaaa tgcacggtct tggatgatct tttgtgcacg
    16621 taattcatct ctacggtgga cgatgactac ttcagaagca aattgtgtca aatagatggc
    16681 ttcttctact gctgagtctc ctcctccgat cactactaga cgtttgttac ggaaaaacgc
    16741 accatcacaa actgcacagt aagaaacgcc acgtccagca aattcttcct cgccaggtac
    16801 gcctaattta cggtggatac agcctgttgc gataatgact gtttttcctt cgtaagtttt
    16861 atcttcacaa ataacttctt tgtggctgcc gtgatccttg atatcttgca cgattccata
    16921 agcattttct gtcccaaatc ttgaaacacc ttcatacatc ttataagcta gatcaggacc
    16981 cattattgaa tcgaaaccag gatagttttc gatttctgcc gtattgttca tttggccgcc
    17041 aggggcgcct tgttcgatca tcaatacgga taagtttgaa cgtgaagcat ataaagcagc
    17101 agtcatccca gcaggacctg cgccaataac gattacatca tacatatgta gattctcctt
    17161 tacttaatca agtaagcata ctttacagcc aatgcgaaaa aaagtctatt gttctgcttg
    17221 cgtttctcga atagctttat tatactcgaa aaatccgctg aaaaacaata gacttttata
    17281 aatcattgat caatcattcg aaagattttc tccatttgaa tcaattactt ttttatacca
    17341 gttgaatgat ttcttcttcg aacgggctaa tgtgccattt ccttcgttgt cacgatcgac
    17401 ataaatgaat ccgtaccttt tcttcatctc acctgtacca gctgatacta aatcaataca
    17461 tccccaagtc gtatagccta atagatcgac tccatctaat tctactgcat ctttcattgc
    17521 ctggatatgt gctgccagat agtcgattcg atagtcatct tccacataac cattctcatc
    17581 gggcgtatcg actgcaccta agccgttttc tacaataaat agtggttttt gataacgatc
    17641 ataaatatca ttcatcgtta ttctcaagcc taatggatca atttgccaac cccattcact
    17701 tgcttccaga tatggatttt tgacagaagc aaagatattt cctgcagtct gttccaagag
    17761 ttcgttgtca gtagtcgaaa ctcgagaaga ataataagaa aaggagataa aatcaacagt
    17821 atgttctttc agcaatgcgg catccccagg ttccattttg atctgaatgc catttcgttc
    17881 catttctttc aatgcataag caggatattc tcctcgtgac tggacatcaa tgaagaaata
    17941 attttctcga tctgattttc tagaagccca aacatcttca ggattgcaag tgtatggata
    18001 atatgctcct gctgccagca tacagcctac tttgttctcc ggatctactt catgagcaat
    18061 cttcgttgcg atggcacttg caaccaactc atgatgtgcc gcttgatatt tgatttgttc
    18121 ttcattttct ccttcttcaa aatacagccc tgcgcccatg aatggtgcat gaaggatcat
    18181 attgatctca ttgaatgtca accaatattt gacaagtcct ttatagcgat tgaagatgac
    18241 tcggcacaga ttttcataaa aaccaacgag ttctctggaa cgccacgcac catattcttt
    18301 gatcaaatgc atcggacaat caaaatgtgt aatcgtcaca agcggctcga tcccgtactt
    18361 ttgacattct ttaaagacat cctcataaaa tttcaaacca gattcatttg gctctttttc
    18421 atcgcccatt ggaaaaatac gactccaagc aatggacatc cgataagtct tgaaccccat
    18481 ttctgcaaat aaagcaatat cttccttata atgatgatac atatcgatcg caatttttgc
    18541 tggataaaaa tgattttcat caaattcaaa cattttcttt tttccagtaa tgacaggaaa
    18601 acggtcttct ccgattggca caacatctac gttcgctaac ccacgtccgc cttcatcata
    18661 accgccctcg cattgattgg ctgctgtagc tccgccccat aaaaaatctt ttctgaatcc
    18721 catcatactt cctccattac atgtgtattg cttgataatt ctcttgccag ttcatcttgg
    18781 acttcgattc cttctgcttt taaccggctg attgtttcct tgaattgcgg taaatactgc
    18841 tgatgtgcaa taaacaactc attcatgatc cgttttgctg tctttcctga tcgtataagc
    18901 ggattgatcg taaacgcttg taaagctgtt ccataatcac cagtcacagc tgcttcgatc
    18961 gtcagcaatt ccatattctt cattacttgc aaccagcctc tttcagcagc aggaagggaa
    19021 ccaaacgcta catttcgtgc gccttgtgcg ccaacatagg cagtgacttc tactacacaa
    19081 tctgccggca gatcaggaac agctccatca ttctttgtag ataccacgat ctgtgtttct
    19141 ttatttccat agatcgaagc aatcgtttca caggcagcat ctgaataata agcaccacct
    19201 cgtttttgca attgttcagg tttatgatcc aattctggat ctttgtataa ttcaaacagc
    19261 tctgcttctg tttctttcac ttgctgcgct cttgtaccaa tcgtttgata ttcttctaaa
    19321 gcatgttcca gcatttcttc ttcacggtag taatagcggt ggtagccaca agggatcatg
    19381 ttcatttgtt ttaattgttc tttgaaaaac gggatatcaa agatattttt cggcaatcca
    19441 ttgtcttcat cgtataaacg atcaatcaat tctgccgtgc ggtcatttcc tttattatct
    19501 gctactttgt gccaatggaa gtgatttaga ccggcaaact tgtagatcag ctcttctttt
    19561 ttcttgccaa gcattgttgg ttctgtcatc atcgccatta ctggcacatt gcataatcct
    19621 atcactttat cccatttgcc atatctgata actgcttctg tcaccatccc gcttgggttc
    19681 gcgaaattga tcagccaagc atttggacat aaacgcttca tgtcttcaac gattgccaaa
    19741 accactggaa cggtacgaaa agctttgaat atcccgccag caccatttgt ttcttgccca
    19801 atcatcccat aataactagg gatccgctcg tctttgattc gtgcatctaa ttgaccgaca
    19861 cgaaactgag tcgtaacaaa atccgcatct ttcaatgctt cttcccgatc cagagttgca
    19921 tagattttga cttcatacgg agaagcatcc cacatacgct gagacatctg actgacgatc
    19981 tcaagctttt cttttccttc ttctacatct acgagccaga tttctcgtat cggtaattcc
    20041 tcatatcttt tgataaatcc ttccatcaac tcgggggtgt aactgctgcc tccaccaatc
    20101 gtagcgattt tgattccttt tgccatgttc tcactccttt actttttaca cttttattat
    20161 cttttttgta aacaaaacaa acaatagaaa ttcgtttctt tccagtaaca tttacaaaag
    20221 ttagtgtttt ctactggatt tttgtaaata tcttgtcaaa aaat
//
LOCUS       DAYYAR010000044        19984 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000044 DAYYAR010000000
VERSION     DAYYAR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.044"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..817
                     /gene="proB"
                     /locus_tag="ACHAT0_001354"
     CDS             11..817
                     /gene="proB"
                     /locus_tag="ACHAT0_001354"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8451304.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            814..2061
                     /locus_tag="ACHAT0_001355"
     CDS             814..2061
                     /locus_tag="ACHAT0_001355"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8451305.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2137..2481
                     /locus_tag="ACHAT0_001356"
     CDS             2137..2481
                     /locus_tag="ACHAT0_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451306.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2598..2924)
                     /locus_tag="ACHAT0_001357"
     CDS             complement(2598..2924)
                     /locus_tag="ACHAT0_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451307.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3297..4499)
                     /locus_tag="ACHAT0_001358"
     CDS             complement(3297..4499)
                     /locus_tag="ACHAT0_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8451308.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4616..5131
                     /locus_tag="ACHAT0_001359"
     CDS             4616..5131
                     /locus_tag="ACHAT0_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8451309.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5330..5776)
                     /locus_tag="ACHAT0_001360"
     CDS             complement(5330..5776)
                     /locus_tag="ACHAT0_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8451310.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(5823..7301)
                     /gene="msr(C)"
                     /locus_tag="ACHAT0_001361"
     CDS             complement(5823..7301)
                     /gene="msr(C)"
                     /locus_tag="ACHAT0_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8451311.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7655..8284)
                     /locus_tag="ACHAT0_001362"
     CDS             complement(7655..8284)
                     /locus_tag="ACHAT0_001362"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8451312.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8268..8732)
                     /locus_tag="ACHAT0_001363"
     CDS             complement(8268..8732)
                     /locus_tag="ACHAT0_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8451313.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9219..10487)
                     /locus_tag="ACHAT0_001364"
     CDS             complement(9219..10487)
                     /locus_tag="ACHAT0_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8451314.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10718..11455)
                     /locus_tag="ACHAT0_001365"
     CDS             complement(10718..11455)
                     /locus_tag="ACHAT0_001365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8451315.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11522..12091)
                     /locus_tag="ACHAT0_001366"
     CDS             complement(11522..12091)
                     /locus_tag="ACHAT0_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8451316.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12219..13091
                     /locus_tag="ACHAT0_001367"
     CDS             12219..13091
                     /locus_tag="ACHAT0_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8451317.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13433..14305
                     /locus_tag="ACHAT0_001368"
     CDS             13433..14305
                     /locus_tag="ACHAT0_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8451318.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14302..16260
                     /gene="nagE"
                     /locus_tag="ACHAT0_001369"
     CDS             14302..16260
                     /gene="nagE"
                     /locus_tag="ACHAT0_001369"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8451319.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16511..17362)
                     /locus_tag="ACHAT0_001370"
     CDS             complement(16511..17362)
                     /locus_tag="ACHAT0_001370"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8451320.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17485..17952
                     /locus_tag="ACHAT0_001371"
     CDS             17485..17952
                     /locus_tag="ACHAT0_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8451321.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18215..18697)
                     /locus_tag="ACHAT0_001372"
     CDS             complement(18215..18697)
                     /locus_tag="ACHAT0_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8451322.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18797..19660)
                     /locus_tag="ACHAT0_001373"
     CDS             complement(18797..19660)
                     /locus_tag="ACHAT0_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8451323.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         5995 a         4122 c         3639 g         6228 t
ORIGIN      
        1 gaggtgtcac atgcgtaaag aaattaaaaa tgcaaaacga attattgtca aagtaggaac
       61 aagtacattg atttatccca atggaaatat caatcttgcg gcaatcgatc agttagcttt
      121 tgtcctttct gatcttagaa atcggggaaa ggaagtgatc ttagtctcat caggagctgt
      181 aggcgttggt atgcataaat tacatttgtc agaacgtccg actttgattc ctgaacagca
      241 agctgcggcg gctgttggtc aagccgaact gatgaatatc tataatcaaa gattcagtgt
      301 atataatcaa cagacggcac agatcctcgt gacaagagac gtggtagatt atcctgaaag
      361 tcgtaaaaat gtgaggaata cattggaaca gcttttgatt atggacacga tccctatcgt
      421 gaacgaaaac gatactgtag cagtggatga attagatcat ctgacaaaat ttggcgataa
      481 tgatcagctg tcagccatca tggcaaaact tgtcgcggca gaccttttga tcgtcctttc
      541 tgatatcgac gggttttatt cagataatcc gactacaaat cccgaggctc agatgtatcg
      601 ctatatcgat aaaatcgatg aaaagattct ggcacaagca ggtggtgctg gtagcacatt
      661 tggaacaggc ggtatgcaga gtaaattaaa agccgcttct aggatatttg aagcaaacag
      721 cgcaatgatt ttagcaaatg gaaaaaatcc ggcaattatt tttgatattc ttgcaggaaa
      781 agaagtaggt actttattta aggaggactt gaaatgactg acttgatgca attaggacaa
      841 caggcaaaag atagcgcgca acaactagca cttatgccta caaaccgcaa aaatgagcta
      901 cttgttcaaa tggcacaaac catcaaaaag aatcaacaag cgatcattga agcaaatcat
      961 aaagacttag aaaaagcagt cgaaaacaat atcagtgaaa cgatgcaaga ccgcttgcag
     1021 ctgacacctg agcgaatcaa tgccatggct acagagatcg aaaaaattgc ttcgttagac
     1081 gatcctattg gaaaagtaga tgagatgtgg acgaacaccg acggattgag aatcggcaaa
     1141 aagcgcgtgc cactaggcgt gaccggcatc atttacgaat ctcgtccaaa tgtcacgaca
     1201 gatgcagcta gtctagcatt caaatcaggg aatgccgtta tccttcgcgg tggcaaagaa
     1261 gcattcttct cgaatcaatt attagtccaa ttattgcagc aagtactggt cagtgctggc
     1321 gaatctcctt atgccatcca gtttgtcgat gatacttctc atgaaactgc aggacagttg
     1381 atgcgattga cagaatactt agatgtgctg attcctcgag gcggagcaaa attaatccaa
     1441 cgagtcaaag aacaggcaac ggtacctatc attgaaacag gtacaggaaa taatcacgta
     1501 tatatcgata aagaagcaca gttatctatg gcagtgaaca tcatcgtcaa tgcaaaagct
     1561 tcccgtccat ctgtctgtaa cgcggcagaa acattgctga tccattctga gatcgcacct
     1621 tttttcttgc cagctattga aaaagaactg gtagaacacg gggtctcttt acgtgcggat
     1681 gctcgtgcgt tagaatatct agagacggct gctttagcag aagacacaga ttgggatact
     1741 gaatatctag attatatcct tgctgtcaaa gtggtcgatt ctttggatga agcaattgca
     1801 catatcaatc ggcataatac taaacattca gaaacgatcg tgacagatag ttatgcggct
     1861 agtcaacgtt tcttaaatga agtggacgcg gcggctgtct atgtcaatgc ctccactcgt
     1921 tttacagacg gatcggtatt tggttttggt gcagagattg gtatctctac tcaaaagttg
     1981 catgcccgtg gtccaatggg gttaaatgaa ttaacttcca caaaatatat catttatggc
     2041 gatggacaaa tccgcgaata atgtttcgtg tgaaacatta ttcccgcctt ttgccttttt
     2101 tctgtaaagg ttctatccaa ctagaaaggt gtcaatatgt ttgaaatttt aatgattttt
     2161 tttatttacc ttatttctct gaatatcgct gcttttctgg gagtcggtat tttatccctt
     2221 ttctttcaat tcaaaaaacg aagcataggg agtcaacggg aaaaatggtc gcagtatttt
     2281 gacaaaatcg ggcaaaaagg gctggttaca cgcttgcaca ttagttacat ggttgcatta
     2341 tgcttattag cgacaatcaa ttactattct tttttcgatc attcaatcgc ttatacgatt
     2401 acgctactga ttgctgggat ttttcactta tcttataaat atcagctgaa taaaaaccac
     2461 ttgaatcgga ctttccgcta aaaaatatcc gtacgataag tagagtgcct tgaagatcgg
     2521 caaaaaactg ccggactttt tagaggctgt gacaaagatt ttgtcacagc ctctttttcc
     2581 atataaacgg cgttcgccta ccagctgcct ggtaaatctg tttcctcttc gttaagaagc
     2641 gaaaatccgt caaatgtttc gccatcataa taacgaagca gctcatccat ggacaagttc
     2701 atcgtttttt ctttttcttc ttccgctgat tcagccagta gctgttcccg atacacctga
     2761 tacataccag ctattttttg ctgcatcgct tttctagttt caccttccgc attgattatt
     2821 ggcaattctt tgatatagcc acgataaatc aaccgatcat ctgaaaattc aatgggtaat
     2881 tcaacgagtg tcaacccgac ttcctgaaaa tattcttcca gcatctatta tccccccatt
     2941 ctttttctta aatgaatttt agcttaccta ctgaaaattt tccagtttca ttattttgag
     3001 aaaaaaaggg aatgtgacaa aaatcgtgtc acatcccctt tttccgaata aatggtgttc
     3061 ttctgccaac tcctcaatca taagtcacaa tattcaacaa acatgagaaa ctgttttttg
     3121 aaattgttcc ttgtgattca gagttaaacg acagtctcac aacctcttgg taaacggcgt
     3181 tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatatgag aagctatttt
     3241 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct gatttcttat
     3301 tcttcttcat ttgtagcaaa ataattacgg tgataaatga tacaacatac actgataaga
     3361 acaatggcac ctgcaaaata aaacgcatag ctttcaccaa tatgttcacc aatcacacct
     3421 gcaataaatg gtgctaaagc tccaccgaaa aagcggacaa aactatagct ggacgaagca
     3481 acgtttcgtt gcagacctgg tatttccata gcaatcgtcg tcaacagtgt attgataatt
     3541 ccttggaaaa atcctgctaa cacaatgctg atcggtacaa ggactggcat agaagttcca
     3601 gctcctaata acagcaaaca aataatgaat aagacaaatg ctgctaaaat tgtccgataa
     3661 gtcgtaaact tccgctcaag agccggtgca acgaaaatcg aagcaatagc cagaagcaca
     3721 ccccagccaa aaaatacaaa accgacttct aattcagaat agccaatcaa taaaaatggc
     3781 gcatacgcaa gtaaagtaaa gaatccgaaa ttatataaca atgcgattag tccaactgaa
     3841 cgaagtttat gattgcttaa cgctttgaca ccttcccaaa ggcctacttt tctctttggt
     3901 ttctcagttt caggtaaaag taccgctagg gctataaaac cgcaaaacat caaaaatgct
     3961 accccaaaaa atggataacg ccatgaacca ctgcccaaga ccccaccgac tagagggcca
     4021 acagccattc cgcagccaag tgctgcttca tacatcatga ttgctttttc tgtgttacca
     4081 actaaaatac tgacgatcgt tgctaaagca gtagagacaa ataaagcatt cccgacaccc
     4141 cagccggcac gtaacccaat cagcattccg atattcccag ataatccgcc aagaccagcg
     4201 aatacaacga tgataaacaa tcctgtcatc aatgtttttt tcaaaccgat ccgactagat
     4261 aagaaacctg taaatagcat caccacacca gtgactaaca tgtaactagt aaataacagg
     4321 gtcgtttcag ctggcgtagc gtttaactgt gcagcaatcg tttttaagat tgggtctact
     4381 agacccactc ccataaaagc gatcatacag gtagctgcta caactagcgc tgaacttttc
     4441 ccctttgttt cttcatttgt ttgttcttgt atatctattt gattctccaa tatttccatc
     4501 cctttctctc tattagcatc atacatgatt tattttacat gtaaatatgc acatgtaaaa
     4561 cgtggaaact ttactgtgat ataataaaga aaataagacg agaggtagga tttttatgaa
     4621 tactttagcc tatatattat tatgcgtgct tttatccaaa ccacgttctg ggtatgaatt
     4681 gaagcagctg atcacaattt tttgggaagc ccatcacagt cagatctata caacgcttgc
     4741 caagttagac cagcagggtt atgtgaccgt tttggacaaa gacgagcact ctcaaaaaaa
     4801 gatctaccac ttgactgatg aaggacgtgc tcttgtagag gaatggatca aagaggaaac
     4861 tcccgctcct acgcaaaaag atgaatttct agcaaaaatc tatgcttttt cgacgctcga
     4921 taaaacaaca gctactggac tgctattcac tagagaacaa cagctgaaca aaatcatgca
     4981 aaaaaatcaa aaaaaattga atgacctctc gctagacaag aaacaagaat ttggacgtta
     5041 tgttgtgatc cagcgcagaa ttatgctttg tcagcaggaa ctccaatggt gtcagcaagt
     5101 aagagaacaa atgcagcagt tttttgagta agggataaac agaacctttc tagaaatatc
     5161 tattgaagag caaattatct tcaataaaag agcaatacag ttggaaaaga aaaagcgatc
     5221 cgaacgacag gccaaaacct atcgttcgga tcactatttt tctagaactg cattcaagag
     5281 actatgacaa aaagcatgcc aaggcctttt ttcaaataac agtgttccat cattcattac
     5341 tttgaattgc ttttagcaca cttcttgaaa gcgggccaac gatgatcaac tgcaacggta
     5401 aagcggcaat cacattcatt ttccaagcat tcatataagc agagaataat tgatcaggta
     5461 ttccttgttc catgatcaca ccgaataaag acatacatgt caccattcct aaaaccatca
     5521 agcatgaaat agttaagatc atttggacct tgctttctgg atttactggt agtttaaagg
     5581 caattttttt cgccagcaca ccgatgatga agacatccaa aatgaaagcc accacgaatc
     5641 caggcactag accaatcaac aaacttgata aatgaagatc ttgatgcagc cataaattat
     5701 acgcgctcat ccccaagacc atcaaaaaac acatcattgt tgtaaaaatt attccttctt
     5761 ttttatttgt aggcaatctg taccactcct ttaaatttgt cacgaaaatc atcgtagcac
     5821 atttaaaaat ttctcgtaag tacttttttt attttctgcc cttttatttc ataaacttca
     5881 tcagccgtac gctcgacaaa gtaagaatca tgagaagtaa acaaaatcgt tcccggatat
     5941 atttgcatta gcttctctaa agcttcgatc gttgccatat caataaaatt agtcggttca
     6001 tctaacagca acacattact tggcttagta aaaagcaaag caatcgtcag acgggtcgct
     6061 tctcccccac ttaatgtaca taacggtttc gtcaaagttt cctgagcaaa ccctaagtga
     6121 ttcagcaaac tacggacaag cgattctgaa aactctgttt gtctcatgac aaaacgaatg
     6181 atggattctt cagatgtcat ttgataaccc ttttgctgat atacttgaaa gcttactttc
     6241 ggagagagaa ggattccttc tccttgtttg cggatatgtt ctaataaggt tgtcttacct
     6301 gaaccatttt caccgacaat cgcgattttt ttacctaaag aaaactgaaa atcaccattt
     6361 accagtaatg ttctcccact tctttccaat tggacatttt gtcccatgat gggatagcgg
     6421 ctgtggagtt caagagattt tggtactgga aaacggattt gtttcatctg ctgcggcttg
     6481 gttgtttctc ctattttttg gagtcttctc tctaatgttt tcgcattctt ttggatggct
     6541 ttttgtacac tgtctttttg tttagaggaa gacaaccgat ccggtctgat ttgttgcttt
     6601 ttctttttac tggacacttt ttccaacttt tccgcttgtt ttttcttttc ttggatcgat
     6661 ttttccaatc gttttttctc tttcatgatc gactctgctt ctcgtagctg agtctttttc
     6721 tccaattcct tttgagtgag ataggcacta taattccccg aaaactctcg gatttctccg
     6781 tcttttactt cccagatttt cgatgctagc tcatctagaa aatagcgatc atgactaaca
     6841 aaaaccagcg taccataata ataacgaagc tcttcaatca gtcgatcaat actttttcta
     6901 tctagatggg ttgttggctc gtctagaagc atcccttgtt cataagaaga aagaagacgt
     6961 atcaactcga tctttcgttc ttcccctccg ctccatttcc gctctgaagt ttcttccagt
     7021 cttaactgga aatgactggc taattccaaa tttaggtcct ttgcttcagc gagataggtt
     7081 aattgaggca ggtagttgaa ggtgatctct gtttggattt tcccatgatc gggaaaaagt
     7141 tcaccggcta ttagtttgag caaagtcgat ttaccgacac cattttctcc tatgatccct
     7201 atacgatctt gctgatagac acgaagagaa tcaatagata gttctagctg gttgccaaag
     7261 ctgacttgta gatttgttat atttactgct aaattttcca tatgttttac actcctattt
     7321 catgtaaaac aacaattatc gatactaaaa aagagcagaa aaatctgttt gcaaaaaaac
     7381 tgcatcacat cttttcctac tctgaacgaa ataactatga ctaaatcacc aaatctgtca
     7441 tagctttcac ctattctact gtcgttcgaa aaaatggaca tactccatcc atgaaattgt
     7501 tgtttataca tgtcttttga atttatttag ttgttattca aaagttcgaa acgtaatttc
     7561 atcgatgcag tcatttttcc gacctccttt tttattaggt taccacacac gattcaaaaa
     7621 aagcaatagc tactgaatag taactgaaaa ccttttattc gaacgtagtg atcttttctg
     7681 gtgctaaacg aacagactcg taaccttctt ctaccgcttt tcctattgat aaaataataa
     7741 ctggaacata acggtcttta tccaaaccaa atgcctctgc caactgatct gcttcgactc
     7801 caccaattgg atttgtttca tagccatgat cacgcgcaac taacatgaat tgcatagccg
     7861 ctaagctaga atcgactttg actacatcgt tcatttcttc tcttgaaaaa ctcttgtaat
     7921 aaggaatgat cgcagctaat tgttgatctc tgacttcttt tggcatcttc cctgattcgt
     7981 aagcttggtt atagatttct tcgccatatt cgtaacattg catatcgcca aaaatcatga
     8041 ccattgcaga agaagtatca ttttgtctcg tattgaaacg gatcaaagga cgcaagattt
     8101 cttttgcttc ctcgctttca gcgaccacaa agcgccatgg ttgcatattg acagaagaag
     8161 gtgcagtcgt cgcttctttg atcatttcca gcatttcttc gtgtgggatt ttgtatgttt
     8221 catcatatac gcgaacggat cttctaccag tcataattgt tgaaaaatca tttttttgtt
     8281 ccattattat taacctcttt cgtttctgtt gttatattgc aaagttgggt attcaattta
     8341 tctaaaagtg ttagaaattg tttcatttct acatcgctga aagccgggga tagcacggaa
     8401 cctgtatctt tatggtccat ttcacaatgc tcgattttct tctgggcttt ttctgttagc
     8461 gctacataca cttcccggtt gttctgagga tttctctgac gcgaaacata gccatttgct
     8521 tccaatacct tcagatgacg agtaactgct ccttgatcaa tctgcaactt ttcttgcaaa
     8581 ctgctttgta gcaatggatt ttcttctttc agtctcatca tgatctcgta tctagtaaga
     8641 cttataccga tattttcttc aaagatctgg acgatttttt gatcggctaa tttgatttga
     8701 tataaaaggt gactaatctc ttctaaagac ataatttcct cctttctaat aattgactcg
     8761 tcaacgattg acttgtcaat aatagaataa aaaaagaaaa aagtcaaaga atcagctgtg
     8821 taataaaaaa aacgctctca atagaaaagt atagagaaaa gaaatttttt gtagaatgaa
     8881 cggaaaaaat gataaagaaa gcacaacgct aaaaccaaga ttattgattc ttatcaaact
     8941 atagaagata gctcttctgc agtagaaaat tttacaccgc caaaaatttc cgaaatcgcc
     9001 ttttgcaaga atcccgtcga atattagaaa ttggatcagt atgcttaaac agtaataaaa
     9061 aagttatgac aagatgttgt cacaaccttt tttgagtaaa cggtgttcaa aaacttgcat
     9121 ctgcggtaat aagttcaacc aaacaaaaaa catgaaaatc tgtttttgtt tggctgttct
     9181 tactacttga tgcagggcag ctttttcact acctcttttt aataaccata aaatcctatt
     9241 ccttcatatc tccagcccat cggtgttagc caatctcttt ccttagcact caaggtatag
     9301 tgatgggtac cagttttttc atttggatta aatagtcgat aaattggctt ggaaccacca
     9361 gaataaaaac ttagaccttc ataacgccaa ccgatttttg ttaaagagtt gatctcagat
     9421 tgtgccattg tgtaatgatg gtctccactg taaggattgt ataaacggta gacaggttgt
     9481 ccgctgtttg gtgcttgcca agcaatccct tcatattccc agcctacagt tggcagataa
     9541 tttttttctc cggcattcaa tgtatataaa tgttctcctg tatgacggtt atacagacga
     9601 tacatcggag aagttgaagt tggtgctgta tcatactgtg tcagattata ggtttcgatt
     9661 acattgttca atgtagaacc ataattaggc gcagtggcat aacgacctgt tagccaagcc
     9721 gtagcatctt tgtaagagtt cgtattgctt ttccatgcac cggaatagta gtaaacgcct
     9781 ggctgaaaag aagtcgtctt caataccgtt acgttatctt gcaatgattc tttgtacgaa
     9841 ggatattttc taaaagcggc attcaccgta atccattgcc cattcacata ttccgaagta
     9901 ggcatcgtaa cagattggcc attatagctt cctttaatcc caaatagatt gtaattaggg
     9961 gcagaagcca aagtactgtt cccccagcca ctttcaacga tggcttgagc aatcatgact
    10021 gatgcatata agtcattggc acttgccaaa ctttgtgcag ttgaggcaat actatcaatg
    10081 aacgcttgtt gagaattagc agatgagcga gcataaaaag catagccagt atagttttcg
    10141 aattcagaca aatctgcttg atgggtatgt gtcaatgttt gcccatctgt caattggttc
    10201 gtgatttctt ctgcattctc tggattgttc tgagcttctg ctgatgtact gtcttcggtt
    10261 tgtgtttgtt ctgttgtttc agttgttggt tcttcggttg aaattgtatt ttctgagaca
    10321 gtagctgact gcaaagtact ctctgtatcg ttttcaattc ctgtttctga agaactcgat
    10381 gtctgagttg cgacagtttc tgtcgattct tcagctagca ctgttgtgtt ggcaactgct
    10441 agatttccta aaagaataac gctaaataat aaattctttg ctttcatctt ttttctcctt
    10501 tgaattatga aacttttcct aaataacagt gtaactaaaa aagtaaaagc aagtcaatgt
    10561 ccatatttaa ataacatttg tttctttttg ataaatttgc tttttctaca taaaaagacg
    10621 taaatgaaga atattcttct ccattcacgc cttaaattga tggtcgtttg aataactatt
    10681 taacaagtaa ttctctttgg atattatccg ttatggatta tacttcctgt gtcgtcggac
    10741 ggataatcat gttactgaca gacatccggt ctggcgtttc tatcgcaaat gctacagcag
    10801 aagctatatc ttcggaggtc aatccccatt ctttttgcgc ttcatgaagt tcaagagcag
    10861 cctttttgtc gctgatggtt tgatataatt cagtttgaac agctcctgga gagatgatcg
    10921 tagatttgat gttattttgc cgttgctctt gacgtaatcc ttccatgatt gcacggacag
    10981 caaattttgt tccgcaataa acggcggaat caggataaac aacatgtccc gccacagaat
    11041 cagttgaaat gatatgccca gatttttgtt tttccatgat tggaagtacg gcggaaattc
    11101 catttaacac acccatgatg ttgatatcaa gcatattttg ccattcatca cggtgacctt
    11161 caactaatgg agcggttggc atgattcctg cattattgta caatacatcg attcgaccga
    11221 acttttccat cgctaaatcg ataactgctt ggacttgtgc aaaatctctt acatctgctg
    11281 tttgaatata tagctcagct tctggtaaag attcttttat tgcttttagg cgatcttctc
    11341 ggcgtgcagc tataactagt ttagcccctt tttctgctaa tagcttcgtt gttgcttcac
    11401 cgattccgct tgaagcgccc atgatgacaa tcactttttc tgataaatca ttcattctat
    11461 tctcctcctg ttttgaaaaa tctcttgctt atactcacta tctcttattt catcttactg
    11521 ttcaaataat ccgttcgaca ggtttgacga ttttggcagg gattcctgca acgatggtat
    11581 tgtctgggac atctttcgta acagttgcat tcgctgcaac aatggcattc tcgccaatcg
    11641 ttacgcctgg cagaatagtt gctcctgctc cgatccaagc atttttcttg atgtggattg
    11701 gctttactaa caagcctctt cgttcttttg gatcggttag atggttcacc gtgatgaggt
    11761 tgacacgagg accaatcaat actgaatctt ctaaacagat gccacctaag tcaacgaaca
    11821 tcacttgacg attgataaag acatttttcc ctatactaat atgtttgccg aaatctgtat
    11881 aaaaaggcag cagaacagtc accgtatcat ctactttgtc actaatgata ttttctagcc
    11941 gtttgcggat atctgctggt tcatggtacc cagtattcag cgctgctaaa ggctcttcgt
    12001 tttcagcttg gatctgatgg atcacttcaa agagcggatc cgttactcga atctctttat
    12061 tgacgatttg tttttcgatg aggcttgtca ttttgctccc tccttttatc cttactttat
    12121 aacggacggc ttatcatgtc taatacttat aaaacatact taactattcc ttttaactat
    12181 acttagtggt atgctttttt aaaaacagga gggaaaaaat ggatattcgt atactgacgt
    12241 attttttagc agtagctaga gaaaaaaata tcagccgagc agcaaaatct ctgcatatca
    12301 cgcaacctac tctctcaaaa caactgaaag atttagaaga agaacttggt gtgacacttt
    12361 tcactcgggg ctcccgagag atacagttga cggattctgg tcgatatctt tattcaaaag
    12421 gaaaagaaat cttgtcgttg gtagaaaaaa cggcgaacaa tttaacaaat gacgcaatca
    12481 tctctggaga aatctacatt ggcggaggcg aaactaaagc aatctctttc atttctgata
    12541 gccttcattc gttgatcaag gcgcatcctg atatccagtt tcatttatat agcgggaatg
    12601 cagatgatat catagacaaa ctggatagcg gattacttga ttttggactc gtaatcgaac
    12661 cgacaaacaa acaaaaatac cagtcatttc gactgccagc ccaagatcgt tggggacttt
    12721 tgatacatcg aaatcatcct ttagcaaagc aaaccgtcat ccatccgcag gaactgaaaa
    12781 ctgttccttt gatcatttcc cggcaaacgt cagtggacag tcaactagct gaatggctag
    12841 gtttcagcat agatcagtta tcgatcgtcg gaacatataa cttattgtac aatgcttctt
    12901 tgatggtcga taacgggact tttggtgcat tagctctaga tgggatcatc aatacttatg
    12961 ggacggaaaa aatattcatc ccactcgatc cgccattaca agcttcactg aatctcattt
    13021 ggaaaaaaga ccagcctctt tctcctgctg ctcgtaaatt tttagatgtc ctaacggaga
    13081 aatacagtta agtagagttt ttttccttcg ctttcagctt tttaacaacg aataagataa
    13141 gcaaaagatc caacgattaa ataacataag tttcttaatt tatttcttcc ttttatttct
    13201 gataattcca gtaaattact ataattataa aaaaagaatt gacaaaacat aactgtctat
    13261 ataaaattga ttacgttaac aataattcgt ttcatgtgac tagatcatac taggcatgaa
    13321 aaatctctga ggaacatggt ttccctagga ggtttttcgt gtctattttt gtttggccgc
    13381 aatcaactgg tctagcaaac gtgaaaacca aatcttcaaa aagggtgcgc gcatgaaaat
    13441 caaaaagatc tttaatcaaa atgcagtatt ggtagatgac ggtggacagg aaaaagtagc
    13501 tattggaaaa gggattggat ttgacagaaa aagaaatgat ttgatttttg atcgtgatgt
    13561 cgaacggata tttgtcatgg aaccagaagg tcaaatcaag ttgcaaaatt tacttaatca
    13621 gattgatgaa cgctttttat ttgcagcaga aacgatcatt gatcatgcgg aaactgtttt
    13681 gatggaaaag ttgaatgaac atgtattgat cgctttgacc gaccacattg ctttttctgc
    13741 ggagaacatt aataacggta tcgtcatccg caacaaactt ttaagagaaa tcgaagtgct
    13801 ttacagtgat gaattcagta ttgctcaatg ggcagtagag tatctgacaa aagagcttgg
    13861 gattccatat acttatgacg aagcaggata tatcgcaatc catattcaca gcggacgaag
    13921 cggtcggaac aataaccatc gcagtatccg tgaagtgacg attgtatctg acatcatcca
    13981 tttagtggaa tcagaattgg aaacagatat ccattcagaa ccattttcac tgaattattc
    14041 aagactggtg aatcatttac gactgcttct acaacgaacc catgcgcaac aatatgctgt
    14101 tttagacacg gaaatcgtag aaatggtcaa acgaaaatac ccagaaagct ataagatttc
    14161 taaaaaaatc cgtgtgttgc tgaccaaaga ataccagctc gcgatcacga aagaagaact
    14221 gggttacttg gccatccata tcgagcgttt aagaagcgca atcgtacaaa caaatgttaa
    14281 caatcatgag gaggaaaaaa attgaaagca tatctacaac gaatgggacg ttcccttcaa
    14341 cttccagttg cggtcttacc tgctgctgct ttactagtag ggatcggaaa ctggtgggca
    14401 tcttatagta acgacattgt cgctcatttt ttacaagctg gcggtaacgc agttttaggt
    14461 caattacctt tgctgtttgc cgttgggtta gcactaggga tgtcagtgga caaatcaggt
    14521 gctgctgcct tggcaggttt ggtcgcattt gaagttccag taaatgtttt aaaaactgat
    14581 tcagtagcta ctttattgaa tatcaaacca gaatcagttg atccatcttt tgctcagatc
    14641 agtaacgtat ttattggtat tattgccgga ttgattgcgg cagctcttta taatcgtttc
    14701 cacgaagcaa aattaccgat ggctttatct ttctttagcg gcaagcgtct agtgccaatc
    14761 ttggctgcct ttgtcatgtt gctgatcagt gcagtgctac tgctcgtatg gcctccggtt
    14821 tactctgctt tagtttcttt tgctaaattc attttgagtt taggctgggg tggtgcaggt
    14881 ctttatggct tcttcaaccg tttactgatc cctacaggat tgcatcaagc aatgaactcc
    14941 gtgttctggt ttgacgtagc aggaatcaat gatattggta aatttttggc ttctgaagga
    15001 acaaaaggcg taacaggaat gtatcaagct ggatttttcc caatcatgat gttcgggctt
    15061 ccagcaggag catatgctat ttacagaaat gcacgtccag aaaacaaagc aaaaacagct
    15121 tccttgatgc tagcagcagc ttttgcttca ttctttactg gtgtgacaga accacttgaa
    15181 ttttcattta tgttcgttgc ttggccatta tatgtcttgc atgcaatttt cactgggatt
    15241 tctttagcag tgagtgcatt cttccattgg acagcaggat ttgcctttag tgccggattt
    15301 gtcgattact tcctatcttt gaaaaatccg attgcgaatc agccattgat gttgatcgtc
    15361 caaggattag taacagcagt gatttactac tttggcttta actttgctat caaaaaattc
    15421 catctgatga ctcctggccg cgaggaagca gatctttcag atgacgatac tgcaacgact
    15481 aattcagaca ataaatatgc ggcacaagca agcaaaatct acgctgcatt aggcggagcc
    15541 gacaatgtaa cttcgatcga taactgtact acacgccttc gtttgcaagt caaagataca
    15601 ggcctgatcg atcaaaataa aatcaaagcc actggcgtac ctggtatgaa gatcatcgac
    15661 ggaaaaaatg cgcaagtcat cgtaggtaca gaagtacaat tcgttgcaga tgagatggca
    15721 aaactacatg gtggtgctgc agcacgacct gcacaaacaa acaccgtggt taaaacagaa
    15781 acatttgccg gtgaaacgat cgaacaagat atttacgcta tcgcaaatgg gaaactgatt
    15841 ccaatcactg aagtttcaga tgatgtattc gcggaaaaaa tgatgggtga cggctatgct
    15901 gtattaccag aaaatggaga aatcttctct ccaatcgctg gtacgatcac aaacattttc
    15961 ccaacgaaac atgctgtcgg aatccaaaca gatgctggta tcgaagtctt gcttcatatg
    16021 ggcatcaaca ctgttgactt aaaaggcgaa ccattcactt tatatgtaga agaaggtcaa
    16081 aaagtcgcaa gaggtcaatt gattgcttta gttgatcttg ccgctattca atctgcaggc
    16141 aaaaacacgg atatggtcgt tgtattcaca aatggcgaca aagtccaaag cttagaaatc
    16201 gaacccgcac gtgatgtaaa agcaaatgat aaaatcggct ctgtttccaa caaagcctga
    16261 gccatcgtct tccccagact tttttatcga taaacaaaca ggagctgaga caaaaatctt
    16321 gtctcagctc ctttttccga ataaaaacgg cgttctcctg ccaactccgc ggtaataagt
    16381 ccagctaaac gaaacatatg aagagctatt ttttatttga ctcttcttat tacttgatgc
    16441 agaacagctt tttcacaaaa tttttcttca tactcaaaac taatcggctt tttcacaacc
    16501 tctttataca ttattcctga tccaatattt cttttaatgc tgcttcaaga gaagttaatg
    16561 gttttcccaa tactttttct aaatcatcag aggaaatagc taactgatcc tttttaatat
    16621 catgttggat cgctaaaaac atgtcagctg cttcttgagg gaatccggct tcttttagat
    16681 tttcgacaaa cgctgcatca cttgcttcaa taatttctaa ctctttacct gtcgcacgct
    16741 ctaatgcctt ggctaattct tcatagtcag ttagattgcc ggataattct aagatttctg
    16801 gagagtcact ttctactagt acttttgctg cagcttctgc atattcacgt ttcaatgccc
    16861 aacctgtttt tccatttcct gcagcataaa cgaattgacc agtttttaaa gcctgcccaa
    16921 tgataggtaa ttcattctct acataccaat tattccgtaa aaatgtatgg gaaatacctg
    16981 aatccttaat cattttttca gtgaattggt gatctggcgc taatacactg atcgagtgat
    17041 cggcatctgc aaaacttgta taagcgatat aagatacccc agctgcttgt gctgccttca
    17101 ctacattctt atgttcggct tgacgatttc ctggaacgcc agagacaaaa agcaatcgat
    17161 ctattccttt caacgcttct ttcattgagt ctaaatcatc ataatcacca atacgtatat
    17221 taaatccagc ttctttcagt gcttctcctt tttcttcact acgtactagt acatagattt
    17281 cattttgtgg aaccatcttt tttaagaatt ccagcgcata accaccaaaa cctccagttg
    17341 ctcctgttac taaataatgc atatttgcca ctccctattc tttttatttg ttaagattta
    17401 cagctgtaca tttataaatt acacctttat cataaagagt gtggtaagat aagtcaagca
    17461 aaaaaaatga aaggatgaca actgatgaag tattctctgc aatttagtga tgcaatccat
    17521 gtacttgcct atattgaaat gtttcaagat acccgatcac tttctagtga gatgattgcc
    17581 agtagtatcg aaacaaatgc agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc
    17641 ggtttgatct ttacaaaaac cggaaaaccg cagccttccc ttgccaaaag tccagatcag
    17701 atttctttac tagatgtcta tcgcagcatc gaaggtaata cacagcttat ccaagtcgat
    17761 ccaaaaacaa atccaaattg cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc
    17821 tacgctcggc tgcagaaaaa ggcagaagaa gaaatggcta agatcacact tgatagtctg
    17881 atacataaaa tagccgtttt ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag
    17941 agatttttat aattctctct actgtgcctg catttttctg atcggaatcg tctacataaa
    18001 ttcttttatt ctccggggga caaaagcctt gtcatgcccc tttttcctct ctaatgctca
    18061 ggtcaaagcg ctgaagtcat gacgtcttga taaacggtgt tcaagtagct gaccctcggc
    18121 attagttctg aaaaatgcaa aatatggaaa gctatttccc atttttcctc tctaatgctc
    18181 aggtcaaagc gctactttca cgacctctat attatcatgg tttattgact accggtatcc
    18241 agatttctcc catatatttc ccgttggtaa ctcccatttc gactacagca tttggacccc
    18301 caacataggc gtaatcttct acttctctta aaacatgccc aaaggccgta ttcgtcaaat
    18361 catttcttaa ttcttcaggc gattctgcta aagcgcttac aacaagatac gttccttttg
    18421 ggaatgggat gattcgcgta gtgtttggaa catcctgact cgtttgaaca ccgacatagt
    18481 acatcatttt cccgttatag ggttcgttga ctgtaaagaa ataatgattt tcagcagctt
    18541 gtttcaatgc atctattgtt ccattaccga taactttttg ctcaaatgct tgtttctctg
    18601 cagccaaacc gacaaaatcc gtattgctac tcttcaattc cacaccaata cccgtcaccg
    18661 taaaatctgg tttttcttcg atcctatact ttgccatttc tcccaccctt tctattatat
    18721 taagttcatc ttacataaaa ttaaagggaa atgacacgat tacgctcttt tttctaggga
    18781 tatttttatt ttttaactat ttttttgctt tcactgcaat ccaaatctca ctgtaaacat
    18841 tactgaaatc ctctccccat tttgtaaaag agatctcggg agcttcgact aacccataat
    18901 ctgaagaagg aagccattct gcatagattt tcccccaagt atcttgtaaa gtttggggaa
    18961 atggcccttt attaggaaaa atggcccaag tgtgttcttc tatagcaagc atttctaaat
    19021 cagcgtacgg attttctttt gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt
    19081 cttccaagcg gtctccatca aatccataag aaacgttgat tacttgattt ggatataaat
    19141 ctcctaactt atgcatctct tccctttgag tctccgtgat cgtctgcgct aacttttgta
    19201 tttcttgatt gactccttca aatttgacag gaactttttt cgccacacct gccacataaa
    19261 actgttcttt cttttcgata cgaaattcca tcgattttcc tcctctgaca tcaataaaaa
    19321 atgtcaagcg tggaaaagat ttttgtatgc cgtatctgct gatttctgaa ggcagatatc
    19381 ctgaccaatc acgaaaagct cttgaaaaac cttcgactgt ttcatatcca tatctaaacg
    19441 ccgtttctgt cactcttgat ccatctagaa gatcgcgatt agccaaagcc aatctacggt
    19501 ttttgatata gtccattagc gacatccctg caataaacgt aaagatgcgt tttaattgat
    19561 attcagagat tcctgtattt tcggctatta ttttatagga aagtccttct gtcaaatgtt
    19621 cttcgatgta atcgatcaat tggttcagat ctttaagcat atttatcttc ctccttgatt
    19681 gcctttttat catattatca aagaaagtca attacttgat gaatcaggaa ccaatttagt
    19741 cagatgtaga aaatgttgta tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga
    19801 taattggtat tatctgccta tctatgtttt acctttagaa aaataaattt tggggaagac
    19861 agattctaag taaacaaagc ggtaaaaata tcttttattc ccttagttaa tccgcttact
    19921 acctactgta caaagcaaat tgttgttcaa actgctgatc agaacgctgc ttggattttt
    19981 cttt
//
LOCUS       DAYYAR010000045        19980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000045 DAYYAR010000000
VERSION     DAYYAR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.045"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(540..1565)
                     /locus_tag="ACHAT0_001374"
     CDS             complement(540..1565)
                     /locus_tag="ACHAT0_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8451324.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1840..3525
                     /locus_tag="ACHAT0_001375"
     CDS             1840..3525
                     /locus_tag="ACHAT0_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8451325.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3539..4822
                     /locus_tag="ACHAT0_001376"
     CDS             3539..4822
                     /locus_tag="ACHAT0_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8451326.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4892..5761
                     /locus_tag="ACHAT0_001377"
     CDS             4892..5761
                     /locus_tag="ACHAT0_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8451327.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5758..6594
                     /locus_tag="ACHAT0_001378"
     CDS             5758..6594
                     /locus_tag="ACHAT0_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8451328.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6620..7687
                     /locus_tag="ACHAT0_001379"
     CDS             6620..7687
                     /locus_tag="ACHAT0_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8451329.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7684..8511
                     /locus_tag="ACHAT0_001380"
     CDS             7684..8511
                     /locus_tag="ACHAT0_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8451330.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8556..9581
                     /locus_tag="ACHAT0_001381"
     CDS             8556..9581
                     /locus_tag="ACHAT0_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8451331.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9578..11761
                     /locus_tag="ACHAT0_001382"
     CDS             9578..11761
                     /locus_tag="ACHAT0_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8451332.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11761..12889
                     /locus_tag="ACHAT0_001383"
                     /pseudo
     CDS             11761..12889
                     /locus_tag="ACHAT0_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            12886..13563
                     /gene="pgmB"
                     /locus_tag="ACHAT0_001384"
     CDS             12886..13563
                     /gene="pgmB"
                     /locus_tag="ACHAT0_001384"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8451333.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13647..14174
                     /gene="msrA"
                     /locus_tag="ACHAT0_001385"
     CDS             13647..14174
                     /gene="msrA"
                     /locus_tag="ACHAT0_001385"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8451334.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14375..15061)
                     /locus_tag="ACHAT0_001386"
     CDS             complement(14375..15061)
                     /locus_tag="ACHAT0_001386"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8451335.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15141..16679
                     /locus_tag="ACHAT0_001387"
     CDS             15141..16679
                     /locus_tag="ACHAT0_001387"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8451336.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16799..17266
                     /locus_tag="ACHAT0_001388"
     CDS             16799..17266
                     /locus_tag="ACHAT0_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8451337.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17800..18228
                     /gene="rplM"
                     /locus_tag="ACHAT0_001389"
     CDS             17800..18228
                     /gene="rplM"
                     /locus_tag="ACHAT0_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8451338.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLAGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18244..18636
                     /gene="rpsI"
                     /locus_tag="ACHAT0_001390"
     CDS             18244..18636
                     /gene="rpsI"
                     /locus_tag="ACHAT0_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8451339.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18725..19951)
                     /locus_tag="ACHAT0_001391"
     CDS             complement(18725..19951)
                     /locus_tag="ACHAT0_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8451340.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
BASE COUNT         6432 a         3616 c         4224 g         5708 t
ORIGIN      
        1 tctaaaataa tttgtctaac tagaagaaaa gtgggaaagg atcttaacaa catcggttcg
       61 tatccaatta aggtatcgga acgatcatct tccaagagaa gtcaatcaac aaaagtaaag
      121 atagatcatt ttttgaatgc aaaataaatt gatgggaaga tagagcggtt atttttaata
      181 ttttgctagc tattattgaa taattcctcg aataaaacaa aaccagtaga catgtctgtt
      241 attctattta tgttttagag ataagtatcc ccaaataaca tggattgaaa taagggtaaa
      301 tgtgtttaaa aaaacgaact ataaaaactt atacacaacg tggttaaaaa tagttttttc
      361 ttccctgtgg aaaaaatagt ttaacacaaa cattatagtt atccacagct atctaaaaca
      421 gatggaaaat aagaaaaatt cgctaaaata ccgggagggg gtaacgaggg atgagtgatt
      481 gaaaaaagaa cagagtaact gacttatcct cagtgtttct gttcttttat aattctgttc
      541 tacatttctt gttgttggat cgaatctgtg agagtaatct cggttgaaag aatgattctt
      601 ttttcttcag ggtattggtg gtggattagt tggaataaaa gttctgtggc tttgattcct
      661 aactgatttg tcggaatgtc tatccgagtc aactgtggcg aaaataggga ggacaataag
      721 ggatcactga atcctgcgat cgaaagatca tcaggtatct gctttccaga ttgttctgcc
      781 actttgtaaa tactcaatga ttttaatgca tcggtagaaa aaatcgcagt tggtctttct
      841 cgtagcttca ttaaatcatt cattgctgcc atgctatctt ctaagcgaaa gccgccgtct
      901 ataatccatt cattacggat atctaggtgg tgatcaaata agcattgacg gtaaccattg
      961 agacgatcat tggatacata aacttctgtc ggagcatgga gacagccgat atgacggtgt
     1021 ccattcttga tcagtgtttc caccaaatcg taactatcct ggaaattatc cgtgtccact
     1081 gagtagatgt tcgtgtagtc gtttccgact tttccgatta ccacaatagg aatattgtat
     1141 tgatccaatt caaaaagaag tgtttcatcc atcgtagagc ttaaaataat aattcctcgg
     1201 atcatttttt cttggatctt tttcgcgcat ttttctagct cttcttctgg tgtttttgcc
     1261 gtttgtagaa tcaagtcgaa gttattttgt tcagaaacgg cagaaatgga ttgaataaca
     1321 tctgaaaaaa aaggattgcc ataggtcgta tgagaagtac gatgagataa tgctagaatc
     1381 gcgtcaaaac catttcctga cagtcctctt gcaattttgc tcggttcata attcaattct
     1441 ttgattgctt gaagtacact tgcttttgct ttatcagaga tatttggctg gttatttaat
     1501 actctggaga cagttgattt tgagacatta gcttttttcg caatgtcata aatagtaggt
     1561 ttcaatttta tatgcacctc ctttttggga gcgttttaga ataattccat agtacaatag
     1621 acattctttt ttgtctatga ttttgaaaat ttaggaatag cttgcatgat catgagagac
     1681 catatttttt taagaagcta ttaattattg atacataagc tttcttattt gtatttttgt
     1741 gtatatcaaa attatttaaa aaaatgctat tgacagtaag cgttttccat aatatgatca
     1801 aatcgttggg aacgctccca aaaaagaaag gggaatccga tgtatacaaa acttttacaa
     1861 gcgatttatc gcgactcaga tttgtctgag aaaaccgcct ctatgattga acaaaaagtc
     1921 agtgtttatc agcagaagaa aggccaggca ggcaaactgc attgggacga agaagatatt
     1981 ttcttgatta cttatgggga tcaatttttt gaagagcagc aaccaacttt gcgtacattt
     2041 aagaaatttt atcaaaagta tttggcagat acatttagag ttgtacattt cttgccattt
     2101 ttcccatata catctgatga tggtttctcc gttgtaaatt atcaggcaat caacgaccgt
     2161 ttgggcacat gggaagatat agaagaaatg agaagcgaaa cacggttaat gttcgacttt
     2221 gtctgcaatc atatgtctgc cgaaagtact tggtttcagg aatatttaaa cttgaatgat
     2281 acgtacaaag atttttttat tgatttgcca ctggatacag atacaagcag cgtgactaga
     2341 ccaaggacca gcccgttact tacaacgttc attgacaaaa acgggaaaga aaaacacgta
     2401 tggacgactt ttagcgaaga tcaagtagac ttgaatttta aaaatccaga agttttattg
     2461 aaaatgatcg atattctttt gttttactta gaaaaaggcg cttcatttat tcgcttggac
     2521 gctgtgggat ttttatggaa agaactagga acttcttgta tacacctgcc agaaacgcat
     2581 aaaatcatcc aattattccg tgcaatcgct gatgaagcag ctccaggaac ggtattgatc
     2641 actgagacaa atgtgccaca caaagataat atcagttatt ttgggaatgg cgagaatgaa
     2701 gcacagatgg tctatcagtt ttcgctacca cctttgaccc ttcaagcgat tcgttcgggt
     2761 aatacaagta agttgaaaga atggttaaaa accatcgatt tcgcatcgaa aaaaacaact
     2821 ttttttaatt ttttagcctc ccatgacggt atcggattga atccggtacg aggtattctg
     2881 ccggaaaaaa cgatccttga attagtggaa cagctgaaga aagagggtgc ccgagtaaac
     2941 tacaagaaaa acccagatgg aacgaccagt ccatatgaga taaacgctac gtatctagat
     3001 gcgttaagtt ctccaacaga tacgacggaa tggagaagca aacgcttttt agctgctcat
     3061 tcatttttgg taacgttacc aggcgtgccg gcaatttata ttcaaagtgt gctgggaagt
     3121 cgaaatgatt acaaaggcgt agaaaaaaca ggagagaatc gaaaaatcaa tcggcaaaaa
     3181 tttttgttag cagatattga gaaagagttg cagacagatc cattaagaac agcagtatat
     3241 ggtgaattga cccagatgct atctattcga aaaaaagaaa aagccttcca tccgaatagt
     3301 ttgatttact tactggactc agcagattct tttatcagtt ttattcgcga agcgaaggaa
     3361 gagaaagtct tagttatcca taatttttcc aatcaatcag caggttatct tttaccaaaa
     3421 ggagattacc tagacctgtt ttctggacaa attatccatt gcagtcaaga acgaaagttg
     3481 atattccagc cgtatgaata tatgtggctg aaaataataa aataagaggt ggaaaaaaat
     3541 gaaaaaaatt attgttacta cctgtctgct agctactgga tttcttctta ctgcttgtgg
     3601 tgaaagtcaa agctctaacg aatcacaaca gacaacgatt caattcatgc actcttctgt
     3661 cgaacaggaa cgattaagtg tgatcaatga actggttgct gattttgaaa aagaaaatcc
     3721 agacatcaaa atcaagcaag tgccggtaga agaatcagcg ttcaatacaa aaatcgttac
     3781 gttagctcgt tccggtaaat taccagaagt aatggaagtc agtcaggatt ttgctaaagt
     3841 gatggataaa gatgaactga ttgatcagac ggcagtccaa aacgttatcg aggaagctgg
     3901 agaagataat tattatgacg gagccaaaaa ccttgttcgt tcagaagatg gaaaaagcta
     3961 tattgctgca ccaattagcg gctgggttca aggtatttgg tatgacaaag aaaaattatc
     4021 agaagctggt ttttcagaac cagaaaattg ggatgatatt ttaaaaattg ctcaacattt
     4081 cacagataaa gagaataaac aatatggaat tgctatgcct actgctgaag gaacaatgag
     4141 tgagcaagcg ttctctcaat ttgctttgtc aaatggagca aatgttttgg atgatgaagg
     4201 cgaggtaacg atcgatacgg aaaagatgag agaagcattg tcgttttatc aaaacttatc
     4261 ccaatacacg atgccaggct ctaatgatgt gacagaaatc aaagatgctt tcatgaatgg
     4321 aacagcacca atggcaatct attcaactta cattttgcct tctgtttatg aagcggggaa
     4381 ctcagaaaat atcggttttg ctatcccaac acaaaaagat caagctgtct atggcacagt
     4441 atctggattg acgatttctt ctggtctaga cgagaaacaa aaagaggctg ctgaagcatt
     4501 cacagctttc ttatctcaac cgaaaaacat ggaaaagtgg gtcttgatgt ctcctggtgg
     4561 tgctcaacca gtgaataaac aagtagtcga actggatggt tacaaagaaa atgaagtcat
     4621 caagtctttt ggcgagctgc catctgagat tgctagtgct tttgatgaag tacaagtctt
     4681 tggactagtc ggggaaaaga atttcacgaa aatgggagat atcacaagct caggtgtgat
     4741 cggaaaagca gtgaaccaag taactgtcgg aaacgaagat gtcgatcaag ctttagctga
     4801 cgcacaaaaa aatacgaaat aacaagaagt tctgccaatg gtgttcgaaa aaagtagata
     4861 gctattggca gattatcagg gggaggtttc aatgaatcgt ccaaggaaac attctgactt
     4921 aaaattagcc atgggacttt tagcaccaag catgctttta ttagctgttt tagtgctgat
     4981 tccaatgctt tccaatattc agatcagttt tttcgatatg ccgctgaatc ctaactcaga
     5041 cgccgttttt atcggattaa gcaattatct tgaaatttta agtgatagtg aattttatcg
     5101 ttctttaggt ttaacagttg cttacacatt cttagttgta gcaggcagta cagctttagg
     5161 attagccgta gctattttgc ttaaccgcaa gttcagattt aggaaaacgg tgcgttcact
     5221 gattttatta agttatgtta cgccatctat ttctttgatt tttgcttgga aatatatgtt
     5281 caataatagt tatggaatcg tcaatgatct gttagtagat aggttgcagc ttttcaacga
     5341 agcaccgctg tggttcgacc agccagttgc tagcttcttt atggtcgttt tgtttgctat
     5401 ctggagatac tatccgtatg cgtttatttc atttttggct attttgcaaa cagtcgacaa
     5461 aacgctttat gaagcagctg aaatggacgg tgcaaatgct tggcagaagt tcaagatcgt
     5521 gacactgccg gcaatcaaac cagtactagc aactgtgatt gttttacgat ctatctggat
     5581 gttctatatg tttacagatg tctatctact gacgaataaa gtcaatatct taggcgttta
     5641 tttgtatcag acagcctttg cgttcaatga tcttggaaaa gcagcagcta tatcgattct
     5701 attgttcatc attattgcag cggtcgtctt gctctcacga aagaaggtgg acttgaaatg
     5761 aaaaataaaa agagcaggaa aatcttattt tacgcgacga ttttgttgct attagctgtc
     5821 aatttgtttc catttttgat catgctgatg acatcattca aggattcaca agaagcaatt
     5881 gcaacgacac aaactatttt tccgaaagaa tggacttttg aacattatca ggatattttc
     5941 aatgctgaga tttttccatt catcatgtat ttcaaaaata gtttagtgat ctccttgata
     6001 gcctccgtaa ctgcagtagt gattgggatc agcggggcat acgctttgtc aaaactacgc
     6061 ttcaaagctc gctcgaaaat caatgctagc ttttacaccg tttatatgtt ttccggtatt
     6121 ttgatcgttg ttccattatt taaaatcatt tccggtctag gtttgtatga taccaataca
     6181 gcactgatta tcacgatggt cgttcaaaca cttcctactg cgatattcat gctaaaaagt
     6241 tattttgaca cgattccgac tgatattgaa gaagcagcaa tgatcgatgg tcttagccgg
     6301 atccagatca ttattcggat cattatacca ttggctattt ctggaatcat ttctgtcttc
     6361 gtttattgct ttatggtttc gtggaatgat tatttatttg cttcgatttt cctgtcgacc
     6421 tccgaaaaat ttactttacc aatcggtttg aacacattat tcagtacacc agactacatt
     6481 tggggacgaa tgatggccgc ttcattggtg accgctttac cagtcatcat tatgtatgcg
     6541 ttatctgaac gatttattaa aggtgattta gtcgaaggcg gcgtaaaagg ataaaaagaa
     6601 aagaaagagg aaagaaaaga tgaaaaaatt agttgcatta gaaccaagaa tagccggatt
     6661 caaagattac gaagaccgtg agatcgaaaa gaacgaagta cgtatccgag taattttcgc
     6721 tactccaaaa ccaggaacag aaattgtcga ctttagagga ttaagtccat ttatcgatga
     6781 agaattttca gaagaatggc gcctattctt gccaagagaa gaaggaagcc aacgcggtgt
     6841 ggtatttggc gaatttcaat taggaaatat gattgtcggt acgatcgaag aaacgggcag
     6901 tttggtgaca gagtatcaaa aaggagagtt agtctgcact tatggaccca ttcgtgaaac
     6961 gctgattaca aacggtgttg ataattataa actacgtaaa cttgatcggt tggatcaatg
     7021 gaaaaatgca gtatgttacg atcctgctca attcgcttta ggtggtatcc gggaagcaca
     7081 tgttcgagca ggtgatgctg tagcaatctt cggtttaggc gctatcgggc aaattgcgat
     7141 tcaattagct aaaaaagcag gtgctactac catcgttggg atcgatccca tagcgcagcg
     7201 tagagaatta gcagaaaaat acggtgccga ttacacgcta gatcctatga aagcagatgt
     7261 cggttttcgt ctaaaggaat tgacagataa acgtggagta gattcagtta ttgaaacgag
     7321 tggcagtgct gccgcattac aagcttcttt aagagggatc ggctatggcg ggatcatttc
     7381 ttatgttgcc tttgctaaac cttttccagc tgggtttaat cttggtcgcg aagcacattt
     7441 caataatgca aaaatcgtct ttgctcgagc atctagtgaa ccaaacccag attacccaag
     7501 atggaaccgt aaacgaatcg aggacacatg ttgggaatta ttgatgaacg gttacttgga
     7561 ttgtgaagaa atcattgatc cagttgtgcc atttgcagaa agcgcagaag cttacatgcg
     7621 ttatgtagac cagcacccag aagaaagtat caaaatgggc gttcaagtcc gaggaggaga
     7681 acaatgaaat tcggtacgca agatcaagca tttttcccaa gagacatttt agaaaaattt
     7741 agatttatca aagactgtgg atttgaagaa tacgaaatcg acggtaagtt gttaatagaa
     7801 aatatcgaca cagtcaaaca ggcaatcaaa gaaacaggga tcccagtagt gacagcttgt
     7861 aacggctacg atgggtggat cggtgacttt atcgaagaac ggcgtcttaa tggtgtacgt
     7921 caaattgctg agattttgcg tgcactaagc gaggttggag gcaaaggaat cgttatccca
     7981 gcagcttggg gaatgttcac ttatcgatta ccgccgatgg tttcaccccg aagcaaagaa
     8041 ggagactata aagcagtcag cgaatcactt cgttatttag ataaagtagc cgaagaaaca
     8101 ggtacggtta tttatttgga gcctttgaac cgataccaag accatatgat caatttgtta
     8161 gcggatgcgc gtcattacat tgactctaat caattgaaac atgtgcagat catcgctgat
     8221 ttttatcata tgaatattga agaagatgat atttcaaaag cattgactac gcaaaaagag
     8281 tatgtgggtc atatccattt agcagataat catcgctacc aaccaggatc tggcgcaatt
     8341 gattttactc gccattttca aacgctactt caaaatgagt atgaaggtta tatggtatat
     8401 gaatgtcgtt tgcgaggaga aaatgaagtg aaaatttatc aggaatcatt gcaatacttg
     8461 aaaaactgtc tggaaacagc gcgtcattta aatgatttag agaaagaata gagaaagaat
     8521 aacaataagt aaataagcaa aggagagaga aataaatgaa gaaagtagca atcataggcg
     8581 ctggacaagt agcagaaaaa gtacatgctg cttattatca aaaaagatca gatgtcttag
     8641 aattggccgc tgtggttgat ccagtcagcc agcgagcaga agacttttgc cgacgtacag
     8701 gattttctca tgcgtataca tcagttacac aaatgctgaa tgagatttct cccgatatcg
     8761 tgagtgtctg tacagccaat cgctttcact tcgaacatgt attggctgcc ctcgaagcta
     8821 atgcttcagt gatgtgcgag aaaccaccag ctatgacagc tgatcaagca agaaagatgg
     8881 cagagacagc cgataaaaac gggcagattt tggcttatgg attccagcat cgtttttctg
     8941 acgaagcaac aatgatcaag aatcatcttg atgttctcgg ggatatttat tacgtagaag
     9001 cgaatgcgct tcgtcggtct ggtgttccag gctggggaac gtttatcgac aaagaacttc
     9061 aaggaggcgg gcctttgatc gattatggca ttcacatgct tgatacagcg atgtatatta
     9121 ctggatttcc taaagtaaaa agcgttcggg cgtacagctt ccaaaaaatt ggtacgaaaa
     9181 aaaattcagg gaaattcggg aaatgggatc caagcaaatt cacagtagaa gatgcaattt
     9241 ttggcacact ggaattagaa aacggcaaga tcatccaact gaatacttcg tttgctttga
     9301 atatcagaga agacaaactg aatgtggctt tttgtggcga tcaggcaggt gctaatctgt
     9361 atccaggcga actattcgaa gatgtagatg gaaagctggt ttccttacaa aagaccacgc
     9421 cagttaatga cccacatgaa ctaagtatgg ctcattttgt gaatcgggcg ctcggtgacc
     9481 agactcagat gattgcagat ggctggcaag gatatgtcat ccagaatctg gtggaaaatt
     9541 tatatcgttc ggcagaaaca ggagaagtta ttaccttatg aaagtagaag aaaaacagtt
     9601 tgatttagag tatatcaata agtatgcgag tcagatgacc attggaaatg gattttttgg
     9661 tatcagagga agccaggagg aagactatcg gaatcaagta aggggaatgt ttgcagctgg
     9721 tgtatataac cgacctttag gagcagaatc agccgagtta gtgaatctcc cagatgtcat
     9781 cagatgggaa ataaaaatcg acggtgaaca tttttctctt gagacgatga aagttcttgc
     9841 atacgagcgg gcactagatt tagaaatggg agagcttaaa cgctatgcgc tcattgaaac
     9901 tgctcaagga aaaaaagtgg aagtcattac ttgtcgtgtt gctgatcaaa ataatctcca
     9961 tcaggcagcc ctgaaagtgg tcatcaagcc gttgaccgaa gactgccaag tagatgtgaa
    10021 aacgggtatc gatggacagc aaaccaattt tggtacacag cagttggttg aaaaagaact
    10081 gcgagtattt ggtgacgatt tgatcagtgc atcctaccag acaacagaaa gtaaaattga
    10141 tattgggatt gcagctaaat tcaataaagc aggtgtattc caagcaaaaa atcgccggat
    10201 caccgcttcc ttttcagaaa agatcagaaa aaatacgtcc tttacgctgg agaagatcat
    10261 cgtgctctct acctctcttc agcaaacaga cccgtttaag aaaagtattg cccaagccca
    10321 gaaaagcgtt agttatacag aaattttaga gcagtcgatg tcttattgga cagatttttg
    10381 gcatcagcat cggataacga tccaaggcga ttcttctttc gatcagttag cgatggactt
    10441 cgctgcttac catttacaga ttatgactcc ccgcaatgat ccaagtgtta gtgtaggggc
    10501 gaagggacta actggcgaag gttataaagg gcatgttttc tgggatacgg aaatttttat
    10561 cctgcctttc tttttatatc agtttcctga agtagcaaag aatttgctgc tgtaccgtta
    10621 tgatcgatta gaaggcgcac ggcaaaaagc aagagattat cactatcaag gtgcactttt
    10681 cccatgggaa agcgcacaca ctggtcagga agagacaccc aaatttgctg cattgaatat
    10741 caaaaccgga acaagacaag aagttgcttc agctgccgca gaacatcata tcagtgcaga
    10801 ccttgcttat gccgttcaag agtatgtcca agcaacagag gatcaatcgt ttttagaaac
    10861 aaagggaaaa acgctcattc atgaaacagc agctttttgg ctgagcagag ctgtaaaagt
    10921 cgatgatcat ttagagatac acagagtcat tggtccagat gaatacacag aatatatcga
    10981 caataatgca tatacgaact atctggcgca ttataatgtg gccttagctg ttaaatatca
    11041 agctggcgat ccgctttttt tgaaagaatg tcaaaatttt ttagataagt tatatttgcc
    11101 agtgcctaac aaagaacagc tgattccgca agatgacacg tatctcagta agccagttat
    11161 tgatttgacc acttacaaag aacgtcctgg cagccagtct attttacttg attatgcccg
    11221 tagcgaagta aatgaactcc aagtcagcaa gcaagcagat cttgtcatgc tgttatactt
    11281 attacctgag tatttttctc aaccaatcgt ttctaagaat ttgcattact atgaaaacaa
    11341 aacaatccac gactcatcgt tgagtaaagc gattcacgcc atagaggctt gccgtatcca
    11401 agaaatggaa tgggcgtatc atttattcca agaagcgtgc ctgattgatt taggaccaga
    11461 tccgcattca tcagatgacg gaattcatgc tgcttcatta ggagcactat ggctagcagt
    11521 tatatttggt ttttcaggta ttacagtaac gaatggaact ctttctattt ctccaagact
    11581 tccttcagca tgggaaaaaa tttcttttcc ttttatttgg aaaaatcaca ggttgctatt
    11641 tgttttgaca aaaaaacaaa tagtaataaa aaaagagacg aaagaaacat tacctatttt
    11701 tgtcaatgga gaccaatacc agctggaaaa agaactgatc ttggaacgga ggggaaaata
    11761 atgcgtattc tgattgcgcc agattctttc aaagaaagtc tgacagcctt agaggcagca
    11821 caagccattc aatcaggttt tcaagaagtt tttgttcagg ctgaatatga tctgctacct
    11881 ataggcgatg gaggagaagg aacggtttca tcattagcag acagtttaag gttgaagcgg
    11941 caaacagcag tcgttactaa tgcatttggc aaaaacaaag aaatccagta cgcttcagac
    12001 ggtcaaactg cgatttttga aatggcagaa atcgtcggat tgaaagatgt cccactaaac
    12061 caacaacagc cacttaggtt atccacgcga ggagtaggag aactgatacg acaattgttg
    12121 caacaaggtg ttcgacacat catcatcggc gttggcggaa gtagtaccaa cgatggcggc
    12181 atcggcatgg cagaaggtct aggttatgct ttttacgatc aagataatca aaaagtagaa
    12241 gcactgggag aaaatctagg taaaattgct acagttgata aaacgaacgt gcctattgag
    12301 ctgattaaag ccaaaatcac tgtcattact gatgttacta atcttttatg cggaaaagaa
    12361 ggcgcttctt ttgttttcgg accacaaaaa ggcttgaaag aaaatgaatt agcagtggct
    12421 gaccaagcca tgcatgattt ttatatgaga tttgcccctc aagtaatcga ggcatcgggc
    12481 agtagtgctg gtggtggaat gggtgcagga ctgttagcat ttgcttcagg gaaaatagtc
    12541 tctggcatcg actacgtatt ggatttactg gagttcgata cgggtacagc aagcagacct
    12601 cgtaatcgta ggcgaaggac gtctagatgg acaaagtcta aaaggaaaag cgccagttgg
    12661 gattgctaga agagtaccag agggcgttcc tgtgatagct atttgcggaa gtgttggtaa
    12721 aggaagcgaa aaagcagcag atgttggcat ttcggccgtt tttccgatcc ttcctgcagc
    12781 tgtgccttta caagaagctt tacagcaagc cactgataat cttcatcgca cagctaaaaa
    12841 catcgcatct ttacttcaag tcgttcattt tcaaggaggt aatcgatgaa aatcaaagca
    12901 gcactattcg atttagatgg cgttcttgta gacacagctc gttatcatta cgaagcgtgg
    12961 ctggttttag ctaaccagct gtccattcca ttcactgaga aagaaaacga gcaactgaaa
    13021 ggaatatcca gaaccgagtc actagagcga ttgctgtcat ttggaaaaat ggaacagaaa
    13081 ttctcagaaa aagagaaatc agcctttgct gagcaaaaaa acaatctata tcttcaagcg
    13141 attcaaaaaa tggatgaaac atccgttttg cctggtgcaa tagctgttct tgagtacttg
    13201 aaaaaaacaa acatcaagat cggattggga tcagcaagca aaaatgcacg cctcatttta
    13261 gaaaaaacga atttgacaag ttattttgat gtccttattg acggcacgca agtatcaaaa
    13321 gcaaaacctg atccagaagt ttttctcaaa ggagcacagc aattgaatgt accgccaaat
    13381 gcatgcttgg tcattgaaga ttcagaagct ggttgtcaag cagctttggc tggtaacatg
    13441 catgtattgg gaataggtga aaacataaat cttccttctg ctgaatatgt gattcctgat
    13501 ttaaccgttt ttgatcaagt acgttcgttt tggcatttat cagaagccgt atcacgtata
    13561 taatagtttc cgttttttct gtcacaagca aaacgttatc ctccttttgg aaatcaaggt
    13621 atgatgagaa aaaaggggga ttttatatga caaaaaaagc agttttcgca ggcggatgct
    13681 tttggtgtat ggtcaaacca tttgaagagc agccagggat catttctgtg acatccggtt
    13741 atactggcgg gcatgtacca aatccaactt atgaacaagt aacaacaggc acaacagggc
    13801 atacagaagc agtagagatc gaatatgatc cagaagttat ttcttatgat cagttggtgg
    13861 aaatctattg gcagcaaacc gatccgactg acgcattagg acagttcgct gatcgcggtg
    13921 attcttatcg tccagtgatc tactataatg atgaagaaga acgaaatatt gcagagcgtt
    13981 caaaacaagc attgcaagac agcgggcgat tcgataagcc aatcgtgaca aaaatcgaac
    14041 caagaagcga attttatcct gcagaagatt accatcagga ttattacaag aaaaacaagc
    14101 tccattacaa tctttatcga gaaggttctg gacgtgcagg tttccttcgt cgacattggg
    14161 gcgtcaagaa ataagaagcc gaaaaagcta cttatggaac accgtatatc agaggttgta
    14221 aaaccagcat tttgagctaa tactgagggt cagctgattg aacaccattt agtggagagg
    14281 ttgtgaacct gtcgcctagc tccaagtcac aaggatttgg taagagaaca ctatatatca
    14341 gaggttgtga caagtcgttt gtcacaacct tttttcaata cctaagatga aggacaatca
    14401 gcaacgcact gctcaaaaaa ggaatgaaag gcagcttttc caactttttt ttatgacaga
    14461 taaagtaaac agaaaaaacg gatattccaa acagactagc aagaaacaag atctgaagca
    14521 actcttctag agacaagcca ccactccaag aaagcatcaa cagagtatcg ccagctccga
    14581 gataggattg accatagcga aggcacacac cgcctacgat aaacgttaac agcacacttt
    14641 gccattggaa gacgccggcc tgcaaccaaa aaatccataa aattgcccaa gaacagaata
    14701 gcgtgtgaga atggaccatg aagtaaaata aatccatcaa cgaaaatagc aaagccgaaa
    14761 gcatccatat gaacgtcgaa aaatttcttg cggaagaaaa agtaaagaac caggcaaaaa
    14821 gaccaccaca taaggcttct gccaacaaat agtagaaggg caaccgacac ctgcaatagc
    14881 ggcagcgaaa tcgttggacg atgatcgaga gaatcggaat caattcatac caagaaagcg
    14941 aacgacagca tttgacacaa tgagatctag gataaataat agagtgacct aacggaatcc
    15001 gctgggcggt taaacataaa aatgatccga tacagcaacc aatgataaaa taaagtaaca
    15061 cgccatctcc cccttttcaa aaccaagata cgcaagctta aactaaattt ttgtacatac
    15121 ataaaatttg aggtgaaaaa atgaacgttt caatcggatt agatatcgga accacacaaa
    15181 ccaaagcagt tgctttcaca gatgaagccc aagaagcagc atccgcttat tttcgctacc
    15241 ctttgatcca agaaacacaa gggatggcag aacaagatca ggaattgatc tttcaaggtg
    15301 tctgcaccgt tataaaagaa gtaattaaac agttgccttc agacacaatc attcaatttg
    15361 tctccttctc ctctgcgatg catggcctga tattgttgga tgaagagaaa acaccgttat
    15421 ctcgaatgat cacttgggca gataaccgag cagcggacga agcagaaaaa ctgaaatata
    15481 ccgtgcttgg caaagaattt tatcggcaga ctgggacgcc gattcatcca atgacaccat
    15541 tgaacaaaat caagtggctg caaaacagcc aacctgaatt attcaaaaaa actgcttatt
    15601 ttgtcggtat caaagactat atcttttata gattgtttgg cgaactgatc agcgactacg
    15661 gaacagcttc aggaactgga tattttgata tccatgaatt ccaatgggca gaagaagtgc
    15721 ttgactactt gaacattcga atagagcaat tgccgcaagt ctttccttcc acctatcaag
    15781 taacaggtct accatcaaaa acagcagcag aactaggttt gccaaatgat atatcatttg
    15841 ttttgggagg ggcagacggg ccgctttcta atctagggtt aggtgctttt ggtgatacga
    15901 tcgccgcttt gaccgtagga acgagcggag cacttcgttt tattactact cagcctcagt
    15961 tccatccaga aatggagacg ttttgttatg ttctggatca gaaacattgg gtagtgggag
    16021 gagcgactag caatggtgct ggcatctttg attgggcagg gcatacattt ttacaggaag
    16081 tcgttcaatc tgctaaagaa aaaggagaaa atccatatga tgcactgctt tcggctatcg
    16141 cttttgtgcc agcgggtgcc aatggtctga tttttcatcc ctatttactt ggtgagcgag
    16201 caccgctttg ggacgcagat gcgtttggca gttttatcgg tttacagcgg caacatacag
    16261 aaaaagaaat gatgaaggcg attctagaag ggatctgtct caacctttat cggattttaa
    16321 aaggagtttg ccaggaaaat cagctcacag agatccgtgc tacaggcggg tttgctcaat
    16381 ctccgatttt tcgtcagatg atggctgatg tactgggtta cccgcttgtt ttccctactg
    16441 tcacagaagc atccgcatta ggagctgtga ttttaggctg gagaagcatc ggaaagatcc
    16501 aaagtatcag tgaagcacag gaattgatag aattgaaaga gcaagttgga gtacaagaaa
    16561 ctgatcataa aaaatatcag aaaatctatc cattatttgt ttcgacacaa aagcatttag
    16621 cacagactta tcagcaattt gctcaactgc gagaagaatt agctgattct gaaaaatgat
    16681 tggctttacg gataaaaaag cgaactgaaa atcgttctca ggtaaatgat tttgttgtac
    16741 ttttcattga aagtctacta ttattttaag tgtaagaaaa tgttaggagg caagaaagat
    16801 gaaattccaa caaaccaaag aagtattaaa tcaattagtt gcagatctaa gccaaatgtc
    16861 tgttgttgtt caccaaacac attggtacat gagaggacca ggtttcctta cattgcaccc
    16921 aatgatggat gagttcatgg atgatttgaa cgctcagtta gatgaagtat ccgaacgctt
    16981 gatcacatta gacggttcac cttattctac attgcgtgaa tttgcagata acacaaaaat
    17041 ccctgatgaa attggccgct gggatcgtac tattccagaa cgtttggaaa cacttgttgc
    17101 aggctatcgt tatctagcag atctttacca aaaaggaatc gaagtttctg gagaagaagg
    17161 cgacgatcca acacaagata tctttatcga attcaaaaca gccacagaaa aacgtatctg
    17221 gatgatccaa gctcatttgg gcaaagcgcc agagatcgac gcgtaaaaac tgttgttgaa
    17281 cttgaccgaa aaagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg
    17341 tcaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga
    17401 acaccgttta ccaagaggtt gtaagcctgc cgttcagctc cgagtcacaa gaaacaattt
    17461 taaaaaacag cttcttatgt tttatggata ttgtgacttg tgaccgagga gttggcagga
    17521 gaacaccgtt tattcaaaat aaggggttgt gacaagactt ttgtcacagc ctctttttgt
    17581 ttttcatttt gacaggaaac gaccctttta tataaaagaa agaaaagaag taaagtttca
    17641 tataaatttt tcaatcaagc agaaaaacgt tgacattgac tagattagcg ggtaatatgt
    17701 taaatggtat tgtttgcccc acaaagagcc ccggaaactc gaagtgtatg tcaaacatca
    17761 gaacttaaat tggaggaaaa taacgtgcgt acaacatata tggccaaacc aggcgaagta
    17821 gaacgtaaat ggtatgtagt agacgcaact gatgttccat tgggacgtct ttcaacagtt
    17881 gttgcatctg tactacgtgg taaaaataaa ccaactttca cacctcattt ggatactggc
    17941 gatttcgtca tcgtcatcaa tgcagacaaa gtgaaattag caggtaaaaa agcgactgac
    18001 aaaatttatt accgtcacag caactaccca ggaggattga aatcaatcac tgctggcgaa
    18061 ttgcgtgcta aaaattctcg tcgtttgatc gaaacttctg taaaaggtat gcttcctaaa
    18121 aacactttag gccgcaaaca atttacaaaa ttgaacgtat acggtggagc agaacatcca
    18181 catgcagcac aacaaccaga agttttagac atcacaaact taatctaata ggagggaatt
    18241 tcattggcac aagttcaata tatcggcaca ggccgtcgta aaaacgcagt tgctcgcgta
    18301 cgtctagtac caggaactgg caaaattact gtaaataaaa aagatgttga agaatacatt
    18361 ccacatgctg acttgcgtga agtaatcaat caacctttcg ctgttactga aacaaaaggc
    18421 gcatatgacg tatttgttaa cgtaaacggc ggaggatacg ctggacaatc aggagctatc
    18481 cgtcatggta tcgctcgtgc attgctagaa gttgatcctg acttccgttt agctcttaaa
    18541 cgtgctggct tacttactcg tgacgcacgt atggtcgaac gtaaaaaacc aggtcttaaa
    18601 aaagctcgta aagcttcaca attctcaaaa cgttaatatt ctttttatat caatatttca
    18661 ggacactttt caattatttg gaaagtgtct ttttttataa taagaacaca tttgagaaca
    18721 catgttaatc acacttattc aatccttttt gaaatgcttc aaaaacagat ttatcaacta
    18781 cgtcaggtgt gttcacgtat atttttgtag tagaaatatc cgagtgcgtt aaagcattag
    18841 agatttgatt catatttgct ccaccttccc tagctatagt tgcataggta tgacgaagtt
    18901 tgtgtggaga taattgagcc aattcaggat gccttttttt tattgcattg attcgatagt
    18961 ttaagtagtc agcgtgaaca ggacaattta cttctccaga aggtttggaa taggtaaaga
    19021 ggtactggtc aggtgtttgt ataattttta gtttcaacaa ttgttcagcc tgagctgatt
    19081 tccattctct taataatgtc atcacaactt ctggtgcctg aataacggtg tctttccaac
    19141 cttttgtatg tgtctttctt tgatatttat ctaaagcata tttgagacga accgtatttt
    19201 tattaaaatc aatatatttc cattgcagag cataagtctc actttttcta tcacctagat
    19261 ataaggtaag catgaaaagt gtgtaatcgt gaagtgtaag caagtgatta tttaagtcat
    19321 tgtttactgc ttctatccaa tcagtcagct cttctgatga taatgcttga ttcccttccc
    19381 ttatcctttt ttcttctaaa gccattttct taggcgcggt aatacattga ataattttgg
    19441 tagttcggtt gaattcgata taacatagta tttcggcaat atcaaacata gagcgaacat
    19501 agcctttgat aatttttata tttgcgtatt ctttcgattt ctttgttaat tcatctgata
    19561 ttatctcagt atttgaattc agataattca ttgcatattc accaaacatt ggaaggatgt
    19621 gcaatcggaa tatatcctta gtattactaa ttgttacatt acttggagcc ctgtttgacc
    19681 tagtagtttg acctagttca tatctaggaa gccattttga ttcataaaaa tctttaaatt
    19741 ttatttttcc tttcaattct agtgagtttg cattctcctc tcttaaaact gtttcaatct
    19801 tctttttcat ttccctttcg gccttaatag ctaattgcct agttggaaat actttttgat
    19861 acttctctga tgcaattcca gtgatttctc tgaatttttt tggatacctc agattagttt
    19921 tatatttccc atttgaaagc tttgtaggca aacttcctca ctcctttaaa aagtaaggca
//
LOCUS       DAYYAR010000046        19507 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000046 DAYYAR010000000
VERSION     DAYYAR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.046"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACHAT0_001392"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACHAT0_001392"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8451341.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACHAT0_001393"
     CDS             complement(1173..1868)
                     /locus_tag="ACHAT0_001393"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8451342.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACHAT0_001394"
     CDS             complement(1881..2597)
                     /locus_tag="ACHAT0_001394"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8451343.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACHAT0_001395"
     CDS             2927..3532
                     /locus_tag="ACHAT0_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8451344.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACHAT0_001396"
     CDS             3857..4180
                     /locus_tag="ACHAT0_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8451345.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACHAT0_001397"
     CDS             4362..5732
                     /locus_tag="ACHAT0_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8451346.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACHAT0_001398"
     CDS             complement(5862..7163)
                     /locus_tag="ACHAT0_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8451347.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACHAT0_001399"
     CDS             complement(7393..9384)
                     /locus_tag="ACHAT0_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8451348.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDKVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACHAT0_001400"
     CDS             complement(9528..9794)
                     /locus_tag="ACHAT0_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8451349.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACHAT0_001401"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACHAT0_001401"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8451350.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACHAT0_001402"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACHAT0_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8451351.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACHAT0_001403"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACHAT0_001403"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8451352.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACHAT0_001404"
     CDS             complement(13954..14475)
                     /locus_tag="ACHAT0_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8451353.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACHAT0_001405"
     CDS             complement(14478..15320)
                     /locus_tag="ACHAT0_001405"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8451354.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACHAT0_001406"
     CDS             complement(15313..16038)
                     /locus_tag="ACHAT0_001406"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8451355.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACHAT0_001407"
     CDS             16214..16510
                     /locus_tag="ACHAT0_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451356.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16766..16940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17078..18670
                     /locus_tag="ACHAT0_001408"
     CDS             17078..18670
                     /locus_tag="ACHAT0_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8451357.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="ACHAT0_001409"
     CDS             18683..19423
                     /locus_tag="ACHAT0_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8451358.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4001 c         3702 g         6224 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt tatcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DAYYAR010000047        19457 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000047 DAYYAR010000000
VERSION     DAYYAR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.047"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACHAT0_001410"
     CDS             133..3660
                     /locus_tag="ACHAT0_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8451359.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACHAT0_001411"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACHAT0_001411"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8451360.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACHAT0_001412"
     CDS             7448..7789
                     /locus_tag="ACHAT0_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8451361.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACHAT0_001413"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACHAT0_001413"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8451362.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACHAT0_001414"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACHAT0_001414"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8451363.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHAT0_001415"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHAT0_001415"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8451364.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACHAT0_001416"
     CDS             13000..13737
                     /locus_tag="ACHAT0_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8451365.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACHAT0_001417"
     CDS             13751..14566
                     /locus_tag="ACHAT0_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8451366.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACHAT0_001418"
     CDS             14579..15226
                     /locus_tag="ACHAT0_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8451367.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACHAT0_001419"
     CDS             15242..15898
                     /locus_tag="ACHAT0_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8451368.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACHAT0_001420"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACHAT0_001420"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8451369.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACHAT0_001421"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACHAT0_001421"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8451370.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACHAT0_001422"
     CDS             18240..18758
                     /locus_tag="ACHAT0_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8451371.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHAT0_001423"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHAT0_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8451372.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4177 g         5398 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa ttgaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagc
//
LOCUS       DAYYAR010000048        18329 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000048 DAYYAR010000000
VERSION     DAYYAR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.048"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..2160)
                     /locus_tag="ACHAT0_001424"
     CDS             complement(76..2160)
                     /locus_tag="ACHAT0_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8451373.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(2302..2424)
                     /locus_tag="ACHAT0_001425"
     CDS             complement(2302..2424)
                     /locus_tag="ACHAT0_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451374.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            complement(2685..3044)
                     /locus_tag="ACHAT0_001426"
     CDS             complement(2685..3044)
                     /locus_tag="ACHAT0_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8451375.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3074..3415)
                     /locus_tag="ACHAT0_001427"
     CDS             complement(3074..3415)
                     /locus_tag="ACHAT0_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451376.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3412..4086)
                     /locus_tag="ACHAT0_001428"
     CDS             complement(3412..4086)
                     /locus_tag="ACHAT0_001428"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8451377.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5262..6560)
                     /gene="asnS"
                     /locus_tag="ACHAT0_001429"
     CDS             complement(5262..6560)
                     /gene="asnS"
                     /locus_tag="ACHAT0_001429"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8451378.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6600..7790)
                     /locus_tag="ACHAT0_001430"
     CDS             complement(6600..7790)
                     /locus_tag="ACHAT0_001430"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8451379.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7811..8338)
                     /locus_tag="ACHAT0_001431"
     CDS             complement(7811..8338)
                     /locus_tag="ACHAT0_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8451380.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8385..11174)
                     /locus_tag="ACHAT0_001432"
     CDS             complement(8385..11174)
                     /locus_tag="ACHAT0_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8451381.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11321..11521)
                     /locus_tag="ACHAT0_001433"
     CDS             complement(11321..11521)
                     /locus_tag="ACHAT0_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8451382.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11974..12294)
                     /locus_tag="ACHAT0_001434"
     CDS             complement(11974..12294)
                     /locus_tag="ACHAT0_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451383.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(12797..13915)
                     /locus_tag="ACHAT0_001435"
     CDS             complement(12797..13915)
                     /locus_tag="ACHAT0_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451384.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            14148..14270
                     /locus_tag="ACHAT0_001436"
     CDS             14148..14270
                     /locus_tag="ACHAT0_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451385.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            14386..15693
                     /locus_tag="ACHAT0_001437"
     CDS             14386..15693
                     /locus_tag="ACHAT0_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8451386.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            15814..17013
                     /locus_tag="ACHAT0_001438"
     CDS             15814..17013
                     /locus_tag="ACHAT0_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8451387.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(17040..18308)
                     /locus_tag="ACHAT0_001439"
     CDS             complement(17040..18308)
                     /locus_tag="ACHAT0_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8451388.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         5250 a         3931 c         3218 g         5930 t
ORIGIN      
        1 cattagttat tttataatcc tatctatttt ctttacattt ttcattaaga aaaggatata
       61 aaaaaaagag gacgttcatt cgtcctcttt ttttacatgc aagcttcgca tggcattcaa
      121 aacagctaat actgtcaccc caacatctgc aaatactgca tctcccattg atgcaaaacc
      181 aaaagcacct aaggcaagta ccaaaatttt tatcccaata gcaaagacaa tgttttgttt
      241 gacgattttc aatgttttct ttgctaaatg gattgcttct gcaatcttcg atggttgatc
      301 attcatgatg actacatctg ctgcttcaat tgcagcatcg cttcccaatc cacccattgc
      361 gattcctaca tctgctcttg ctagcaccgg tgcatcgttg attccatctc cgacaaatgc
      421 ggtcttttta ttgtttcttt gtaagatttc ctctaaccgc tgcactttat cttcaggaag
      481 caattcgcta tatatcttgt ccacgcctac ttgcttcccg atatgatcag caattttttt
      541 cgagtcgccc gtcaacatga ctgtgttctt cacaccagcc tgttttaatt gaaccaatgc
      601 gtctacggca tcttctttca aggtatctgc aataacaaga taaccactgt aactttgatc
      661 gatcgctaga taaagcaatg taccgatttc ttgacttaaa ggaaaagaga tttcaaatct
      721 ttccatcaat ttgtgattac ctgctaatac ttgtttgcca tcaacttcag ctaagatccc
      781 ttcacctgca aattcctgga tattagttgt tggtaaaagc ggacctgcgt agccttctaa
      841 aacagattga gcaatcggat gtgtagaaaa ctgttctgca ctcgcaatat attgcatgaa
      901 tttctttgga tcgatttttg tatcgatcgt ctggatcgaa aagtcacctt tcgttaatgt
      961 gccggtttta tcgaatacca ctgtctcggt ttgcgcaagc aactccaagt aatttgaacc
     1021 ttttactaat acaccgattt tacttgcgcc tccgataccg ccaaagaaac taagtggcac
     1081 ggaaatcact aatgcacatg gacatgagat cactaaaaat gtcaatgctc gatagatcca
     1141 ttcagaaaac gccgctccag aaacaacaag tggaggtaaa acagctaata aaacggccaa
     1201 tcctacaacg actggtgtgt aatagcgcgc gaatttagta atgaaatttt cagctggtgc
     1261 ttttttactg ctggcatttt ctactaattc taataatttg ctgatagtag aattttcata
     1321 agatttttct acttgaacta aaaatggttt atttttattg ataaatccgc ttaatactgt
     1381 ttcaccaaca tatacagatt tagggactga ttctcctgta agtgctgacg tatcgactaa
     1441 tgattctcct tcgatgatcg ttccatctaa cggtacacgt tcaccaggct tcacaagtac
     1501 aaactgaccg atctgcacgt cttcagggtt cacggtcatc aatccttccg tcgtctgcac
     1561 gtgagctgtt tctggacgga tcgccatcaa ttcgcgaatc gattttcttg aatgactgac
     1621 tgccaatcct tggaaatact cgccgatttg ataaaatagc atcactgcta cggcttctgg
     1681 atattcaccg atcaacatag cgccaatcgt agcaacagac ataagaaaat tctcatcaaa
     1741 gatttttcct ttgacgatat tcatcactgc cttcttcact acatcatatc ctatcagcag
     1801 ataaatcaat aaatacccta tcaagcgaat tggttcatta aaatcaaata atagcaataa
     1861 agcgatacct gcaagggaaa ataaaatccg gaacagcgta tttctctctt tttcatttcc
     1921 atgttcagat actttttcct tatctatttc tgttactttg acatctggtt ctaattcctt
     1981 cactactttt tttgtttctt gttcaatcgt agccaaatga tcttcctctg cgtcaatcgt
     2041 tagttttcca gaagtgaagt ttaccgttgc ttccttgact ccattgatct tctgaactcc
     2101 tttttcgatt ttcattgcac agttcgcaca atctaaacct tctaatctgt aacttttcat
     2161 tttaatgacc tcctcgcctc aacatatgaa catctcttca taactaatta taaatgaatg
     2221 gttattcata tgtcaaggta tccgaaagaa aaaatgaaaa acatctgcat cggaaaattt
     2281 cttccataca gatgttttgg cttattcttt ctttttctct tcgatcattg cccctgctct
     2341 tatccaaccc gcacttgcct tcttgatctt gacaggatag caactaagtt tgaagcctgt
     2401 tgctggcaat ttgtctaaat tcattaattt ttcgatagga caatatcttt tttcaatccc
     2461 tgcaaaatgt ccttcccaga tattcgaagc tccactacgc tttcaaaaat atagaaagag
     2521 ttctttaatg aaaattattt caactttcca taaacagtaa tcccatataa ataaaagacg
     2581 taaaagtcac atgggagaac ttatacagga aaacacatag agtcaggtga aatacatgta
     2641 tcccacctga ctctatgcgt tttctaacct ttattattct ctttttatac aatttctgca
     2701 ccgaatggca tcaacgccaa tcgagcaagt ttgaaagatt gcttggcaaa aggtatccct
     2761 atgatcgtga tacatagaag gatgccgctg acgacatgtg cgagagcaat tggtaggcct
     2821 gaaacgatca gccagatgat attaacgatc aatgacatgc cagactggtg ataaattact
     2881 tctttgccaa aaggcgcaaa agaaagggag gcgatcttaa aacattgcat accgattggg
     2941 atacctatga tcgtgataca ccaaagtatc cccgctgcta accagctaag accaccgatt
     3001 agtccgccga atataaacca gatgacattt cctaaacaac tcattgtatt tctccttcta
     3061 ttactgtttt tgattaaaac tgggcaaaaa ttgattttat tcttcttttt ttagcttcca
     3121 gcatcaagta ttctgcaaat tttttttgga tttcttccgt attttctttt gttactaatg
     3181 tgtgttggat tagttttcct gatggtcgct gatccacccg ttcttctgac tgttgcacgt
     3241 ccgcttgcgc aaatccgata atccgttttt cttttgttac agcaacgagc aatagtttat
     3301 tcttatcatt taggtcagct gccgtttttg cttttacgaa tgattctgga tgatcagtaa
     3361 tcagctgatt gatttgaggt aaatcttctt ttactgccca ccgtatttcc atcatttctc
     3421 acttttcttt cgctcatttt tatctccact aaaaaaatcg actgcggcaa ttgcatctat
     3481 ccagctttgt gcaatgatct catgtccgcc aatcgttggg tggaccccat cttctcttgt
     3541 atatttcgaa aaaccataag caatccctaa agcattgaat atgccatcta aaggaaccag
     3601 caatgcgcca tattccttag caagtttacg aacaatttgg attttagggt ctaagtcttt
     3661 acgccagcca tgacggtcgt cggttatagg aagtacaaac ggctccatca agatgatgtt
     3721 gcagatttcc gctgtttgaa gttcatttag taattttcga taaacggatt caaattgctg
     3781 cgcagatttc tcagttccga atatagcctg atcgctagta ttatgccagg tatcattgat
     3841 tccgatcaaa atcgatacat agtcaggttg taaagctaaa caatcttctt gccagcgtac
     3901 ttgcagatct gcaattttgt ctcctcctat cccacggttc atgcattcaa atttcatcgt
     3961 tggatattgg gagattagtt ttccggcaat caaaagtgga taacctttac ctagatcagt
     4021 tgcttctgct cggtctcttc ctgcgtctgt aatgctgtca ccgataaata atatacgttt
     4081 tttcatttgt caaaaactcc tttttgaata aaacgatgat tttcttcctt atattatccc
     4141 ataagcccta tcaagacgaa agagaaaata tatgtataaa aaatgacacc agcctagtcc
     4201 cttcaatcaa ttgaatgaac gctcatcaag tgtctagttt tttatacttt ttttcttaac
     4261 gtgccggatt ccccttgatt ccttcgttgt ttggtccaga gaaatcgatc acacctaagc
     4321 ttccgtcaat cagaccactg atgacgccat cagaacatat atttataggc tcatctaaga
     4381 cttccagtaa atgttctttt actaatatct cttctagatt gcttgggata gattggtcat
     4441 aactttatct ggtccgatga tttcaaggat gaattaaact tacatatgga tcattgcatg
     4501 ctgaaaatac ggacctactc catcttcgaa tgttactatt agtttgtttt caggtactag
     4561 atgctgcttt atttcaacag cagaagcatc atcaaatcag atttccattt gaataacctt
     4621 ctttgtatat gtttgattga ttgcgatttc gttatagaac cttgtgaatc aaatagcaat
     4681 ttcttttgct ttcttctctt gtctgctgga aacttccttt atcccgtatt ttaaaaaaag
     4741 aactgcgaga gttcgcagtt ctttttttta aataaacgtt gctctccggc caactcctcg
     4801 gtcacaagtc acaatagtca tcaaacatga aaaactgttt tttgaaatta ctccttgtga
     4861 ctcggagctg aacggcaggc gcacaacctc ttgatataaa cggtgttcaa tcagctgacc
     4921 ctcgatatta gctaaaaaaa cggtaaaata tgagaagcta tttttccgtt ttgtttctct
     4981 aatactcagg tcaaggcact gatttcacaa cctcttcctt aaacggtgtt caaaagccag
     5041 tttctcggta ttaagccaaa tttatgcaaa aatgtggaaa gccatttcca caaaattttt
     5101 cttaatactc gaaactaaac agctttttca caacctctcc actaaacggt attcaaaagc
     5161 ttacatctgc ggtaataagt cccgccaatt caaaaacatg aacagctgtt tttgcttggc
     5221 tgttctaatt acttgatgca gaacagcttt ttcatcacct cttatggata aattcggttc
     5281 aacaaacgtg ggaatggaat agcttcacgt acatggtcga taccagcaat ccaagtgact
     5341 gttcgttcaa ggcctagacc aaaaccagag tgcggaacag aaccgtattt acgtagatca
     5401 agataccatg aatattcttt ttcatccaaa ccgtgattac ggatttgttc tagcaagaaa
     5461 tcatagtctg ttgcccgttc gcttccgcca ataatttcac cgtaaccttc tggtgcgatc
     5521 atatctgcac aaattaccac gtcttctctt gttggatgtg gtttcatgta gaatggcttg
     5581 atggcttttg gatagtttag gataaagaca ggctgttcga atgaattcgc aatgaacgtt
     5641 tcatgaggtg aaccaaagtc ttcgccccat tcgatgtcat caaatccatt tttcttcagt
     5701 aattcgattg catcatcgta agtaatacga gggaatggca attttgtata agtttttaag
     5761 acttctttgt ctcgtcctag tacatctaat gcatattcgc aatgatctaa aacattttgt
     5821 actaagaatg ctacatattg ttcctgtact tctaaacttt cttcttgatg agtgaaagcc
     5881 atttctggtt cgatcatcca gaattcgatc aaatgacgtc gtgtttttga tttttcagca
     5941 cggaaagtcg gaccgaatga aaagactttt ccgaaagcca tagcagcagc ttccatgtat
     6001 aattggccac tttgagataa ataagctttt tgatcaaagt aatttgtttc gaatagatcc
     6061 gtagtgcctt ctggtgcaga accagtcaag atcggcggat cgattttgac aaaatgatgt
     6121 tgattaaaga attcatacgt tgcacgaatg atttcatttc tgatctgcat aatcgcatgt
     6181 tgtcttgaag aacgcagcca taaatgacga tgatccatca agaaatctgt tccgtgttct
     6241 tttggtgtga tcggataatc gtggctctcg cttaccactt ccacatttgt aattccgatt
     6301 tcataaccaa attttgaacg actgtcttca cggatttctc cagttactaa cacggatgtt
     6361 tcttggtgta gatttttagc tgtttggaaa acttcttccg acacttcgct ttttacaact
     6421 acaccttgga aaaaggctgt tccgtctctt aattgtaaaa atgcgatttt tccgcttgat
     6481 cgcttatttg cgacccaagc gccgattgtt actgtttctc ccacatggtt tttcgaatcg
     6541 atgatttgaa tttgttccac gtatgactct ccttttatat tccttgaatt tcttcaggat
     6601 taattttgac ttttactctc aataaattgc ttgattctgc gtaccgcttc ttctaacgta
     6661 gcgcgatcag tggcataact catgcggaca ttttctggtg ccccaaagcc ttcgccagtc
     6721 acaagcgcta cccctgtttc ttctaaaaga tcttctaccc attgtgtgac attttcatac
     6781 ttacacatgc gtagcgtttc ttttacatta ggaaaaagat agaaagctcc ttgcggcttt
     6841 tcaagagaaa cacccggcag ttctgccaca agcggatata acgtattcag gcgttcttca
     6901 aaggcgttcc gcatttcttc tactgtatct tgctcacctt tcaatgcttc aacagctgca
     6961 tattgactga ctgctgtcgg attgctagtt gattgggaag caacagcgat cattccattg
     7021 atgatcacag gatttcccac cgcataacca atgcgccagc ctgtcatcgc atatgtttta
     7081 gacacgccgt tgatgatgat ggtctgattt ttgattgctt ctgaaatcgt tgcaattgga
     7141 gtaaactcat tgccattata aactaaacgg ccataaatat catcagcaac tatcaaaata
     7201 tcatttttta ctgcccattc accgattgcc tgcagttcat tcttgctata aatcattcca
     7261 gtcggattcg atggagaatt gatgattacg gcttttgttt tttctgaacg tgcttgttct
     7321 agctgttcaa cagtaacttt ccagctattg gattcttccc ctttcacaaa gacaggaagt
     7381 ccttctgcca gttttacttg ctcaccgtaa ctgacccagt aaggcacagg aatgatgact
     7441 tcatcttgcg ggtcaaggat cgtttggaaa agggtataca aagcaaattt cgctccgtct
     7501 gttacgactg tttgtgaagg ttcatagaca agtccatagt ttttcttcag ataatcaacg
     7561 attgcctgtc gaagctctgg gattcctgct gttggtgtat aatagctagc tttcccattg
     7621 cggattgctt cgattgctgc ttcttggatg ttttctggtg tcgcaaaatc cggttctccc
     7681 acagtcaaac tcagcacatc tctgcctttt gcttttaatg ccttggcctt tgccgcagct
     7741 gccaaagtga cagacggttc taatcgtttt gttctttttg ataactccat cccattcatt
     7801 cctttacctt ttaaattccc ttgattgtct tgacttcatc tccagattca aagccaatca
     7861 ggtaataatt cagtttccca tttgtttctt ttcctactac ttcccacaca ggctgatcat
     7921 catacatccc taaagcagct ttttcgattt ggatttcagg atgggccgca gcaattgtct
     7981 gttttgcttc gttttcagtc agtccctttg tttgatccaa tacttttact ttttcaccag
     8041 atttaggaat gataacggcg atttcttttc ctttatcatt tgtacctgtc agtgaaaagt
     8101 atgtcttctt tcgagtaaac caataaaaat gatcggtttc ctcaagattt gcatatttct
     8161 ttgcgatttc tactgcttct ttcttcgcct gcaccgccgg acgactggaa cgcaagtaaa
     8221 aaatcgagct gatcacgatg accgctaata aaaatacgat caagccaagt aacacgcgat
     8281 taatcgtttt ttcctttgtc tgttttttct tcaagggtcg tcgctccttt atcttcattc
     8341 cttttcatta tagcagattt taattatgaa aaaagacagc tcctctaact tccttcttca
     8401 tttttcttta agaattcatg gatatcctga tagatttcct ccatagctag ctctttgatc
     8461 ggtacttttt tcggtaatgc atggatgatc cgtgcgccat attttgcagt gactagtctt
     8521 cgatccaaaa taagcaatac gccacgatct gtctccgtcc ttatcaagcg acctaatgct
     8581 tggcgtaact tcaaagctgc tttaggtacg gattcctggt aaaaaggatt gatcccctct
     8641 gattctaaat aagcgttcct agctttgacc atcgggcgaa gcggattatc gaagggtaaa
     8701 cgtgtgacta caacgatctg caaagcatct ccaggcaaat ctacaccttc ccaaaagctg
     8761 tcagcgccaa acaatacgct tttttctgac aaaatgaagc gtttcaaaag cttctcccgg
     8821 cttccaccaa acccttgagc tagtatttct cgtccttttt ctagcataga cagatgcatt
     8881 ttctgataga ccttctgcaa aacatcatga gaagtgaata acacaagtat tggccgatct
     8941 tcttgttcga tcagacctgt aagtgctgtc tctaaataat cgatatatgc ttgattgctg
     9001 acttgatgga cggcaggtgc ctcttttggc acatagatcc ttgcctgttt ctcataatca
     9061 taaggagagg caatcacttt aagaggtgct gcttcaatgc caagcctctt agagagatag
     9121 tgtcgattag ggccgatctt cagcgttcct ccaatataaa gaatacgatc atatcgagga
     9181 taccaaacag tcgacggcaa catagccgct tgaaaatcat ggagatgaaa aatcatctgc
     9241 tgcgctgtgt tcccaggaac gaaccaatgg atcaaatgat ctcgccagtc ttccagccat
     9301 tgatccatca gcaagtattg cttgtctaat tgatcaaata ggctatataa acttgctaaa
     9361 tcgatccgca tctggtttgt ccagctttct tggataatct caaaagtcag acgcatttgt
     9421 gactgtagct gctggatctc ctgataaaaa agctgcaggt gatcaatggc tgtcccagat
     9481 tccagtggca gctgatccaa catctctttt gtcatcatga cttcttctgg atactgtccg
     9541 ttaggttgct tgccgctcac taaactcatt aatccttctg ccatacaaat ctgtgcttcg
     9601 catagcgcag aaagttcttc tcgataaatc gataaccagc gtaaggcttc atgttctttc
     9661 ggtaaatagc tttgtattcg gtcaaacagc tgatttggtt cttctaattg ctggatttgt
     9721 ttatggaaat agttaatact taatcgtttg tcatttactt tttcagcgat ttccggcaaa
     9781 tgatgtactt catcgatcaa caaaaatgga ctagctggaa gcaatggttc ttttcgctgt
     9841 gtttcttgta ctaaaaacgc atgattgaca atcaaaaaat tactttgcct gattttcttt
     9901 tgcaagaaac gaacaaagtc ttgttcgtaa aatgactgat ttcttgctaa aaatccaata
     9961 cctctgtgcg cgatatctgc aaagaatgaa tggttcaaat ttgttaaatg taattcatcc
    10021 aaatcaccag tcttcgtttg cgtcagccaa actaagaccg ccatttgata caaagcatat
    10081 tgtttttgtt ctggcggttc cacaagtgtg cctttaaaac gctgcaaatc aatatagtgg
    10141 cgatagcttt tgataattac aggttgtatg ggctgatcta gcaattgatt cagcaatgga
    10201 atgtcttttt ctatgatctg ctgctgcaaa actagcgaaa cggtactgat gacagcgggt
    10261 ttttcaggtg tagccaaata actcaatggc agcagataac cgattgtttt acccattcct
    10321 gtagctgctt cgatcatcaa atcttttgat tcatcttttg tgaaatggtc gtagaccgca
    10381 ttcatcagtc tgttctgttc ttttcgatac atcagtgttt taccaaacat tttttctttt
    10441 gcttgttttt ttcttggata tgtccgctcg ccataatgga cggatgtgaa tagttccact
    10501 tcttttttct gcaaagctag cccatcgacg atttctaatg agggatcaag tggctctggt
    10561 ttttctttca tttcttcaat cacatgataa atgaaacgac tcgtatccat acctgtgtgt
    10621 ctacttaatc gcgcaatctg ctctaacgtg atgattggca attttctcat ccgttcttcg
    10681 atcagcaaaa gaagttgacc tgtcacttcg gcatcactat ctgcctgatg aggattctca
    10741 tgaataaatc caaggctttc tgacaaatca gctaagcgaa agcttggttc cgtcggcaag
    10801 aaaatttgtg caagttctac tgtatcgatg cctggtatct tcaaactagg aagtccgcat
    10861 cgcatgagtt catggttcaa aaaattataa tcaaaataaa tattatgcgc gacaaatatg
    10921 gtgtctgcca aaagattgta aatcgtttgg gcgacatctt cgaaataagg cgctttttgg
    10981 acgcgttgat tcgtgatccc tgttaaatgc tggatttgtt ttgaaatctt ccgccctgga
    11041 tgaatatcga tcgaaaaacg tgaagtgatc cgacctgatt caatcatgac gcagccaaat
    11101 tggatgatcc gatcttcttt tggattcgtc ccagtcgttt cgatatcaac gactgcataa
    11161 gttcttggtt tcaaaatatc ttcctcttct cctgcctcta atgttctttt gaaattatac
    11221 tacaaagtgc agaaaaatac tctcatcgct ttttaaagaa aatagacaaa aacgcccaga
    11281 cacgcaaatg tctgggcgtt tattttttag tataggttaa ttaagcttta gtaacgtttg
    11341 ttgcttgagg tccgcgttgg ccttcttcaa catcgaaagt tactgcttgg ccttcttcta
    11401 aagttttgaa tccgtcgcct tggatagctg agaaatgaac gaatacatcg tttccgtttt
    11461 ctgcagtgat gaatccaaat cctttttctg agttaaacca ttttactgta cctgtttcca
    11521 taataaaaat cctccttgtg tatcaacaca tattttgcaa ttacttgaat ggtgtatatc
    11581 tggaagatgt ttatttgaaa caagctacca cagattaccg aacaaaaact actcctatag
    11641 tatatcatac cgtttacaaa aaagctagag tttttcgact ttttttatta aaaaaagttt
    11701 tctcagcgat tacataaagt gaaaaaccga actttttttt aaaataaacg acatattgtt
    11761 cagttggtgt ttttttgctc tgatgttctt ttttaaacta aaaaaattat tctttctgac
    11821 tacttatcgg ttctgtttca acggtcgagg ctggggaaga ttctttagta ggtctgatgt
    11881 tctcatggta tttctgatag cctatgacag ttaccagaag aacaaggcaa accaatacga
    11941 ccaacgagat tagtttttct tttttgctca tatctatacc tcataaataa caggatgatg
    12001 tccatcatat ttctgagtat gagtaaaagc aaaagctgca gctgtagaat gtaagaaatg
    12061 ccagcaactt tcataatcac aggtcggaca gtaccaaaag taaccaaatc ttggctgcaa
    12121 agaccgttct tctgtttcag gaatcgcaga atcaagatct ggtgtctgta cataaacatt
    12181 tttgtacaga cgtgtgcctg taataggaaa ccgctgtgtt tcttcagcaa aaactggctg
    12241 acttgttaaa cttagggata gagacagcaa acaccataac agtctttttt tcataaagac
    12301 ctccttatcg ataagttttt cccaattaaa atcatatctc aagaacgccc gttattcaca
    12361 ttttgttcat aaatgatgtt ttttgtatca taatcgtcaa aaagaggtcg tgaccacgtc
    12421 acaacctctt ttttaaacgg tgttccagaa gtagcttctt cggaaataag ccgaaatttc
    12481 acaaaaattt gagaaataat ttttgtcaat ctcttcttat ttcttgaagc tgaccacttc
    12541 tgtcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagcccacc
    12601 aaacaaaaaa tatgaagagc tatttttgct tgctgttctt attacttgat gcagaacagc
    12661 tttttcacaa cctctcatac acggtgttta atcagctgac cttcggtatt agctcaaaaa
    12721 acggcaaaat atgtgaaact atttttccgt ttgtttctct aatactcagg tcaaaacact
    12781 gatttcacaa cctcttttaa taacgaagaa tcgctgtcag actcttttca tctggcgcat
    12841 ctttttgatc caaaataaac acttttccgc cattaatgat cacatcatct gctaatgcat
    12901 tcaacacttg acgccgatca tattctgttt caggatcttc accgaaacca tcagctaagt
    12961 ttgacgtagc gataaataaa tgtgacactt ttccttcttt cgcagctgga acgatatcaa
    13021 ctaactgatc gatgaatttc ttatctaaga gtttattgta ttcttttgat tcttgagctg
    13081 cgagttcact tgacagcttc ttcgcccctt cttcgatatc tctaaaggat agctgcgcag
    13141 gagaagcaga aatcgcaacg tctgcacaat aatagggatt tttagcaatt tttttgaaca
    13201 tcgtctgatt ttctggtaga gcaaataaat aaatcggcca ttgttctgga ttttcgaaag
    13261 aatccttcaa gtacgtatca actgcttggt aatagttgac ccaatcgatc tctacttctt
    13321 catcttttgg atttacgcca tggaaagtta ctccatctcg ggctccgcct cctccattag
    13381 aagagaagtt caaaccgcca cctgtcagtt cagatcccaa tgcagtaacg acatcaaccg
    13441 gtgccccttc tggtagagat acttctttta cttgtttgtt gctgacttca tacaatttca
    13501 tggagtcgcg gttcaatccc aataaataat aagaatagtt gaactgccca tttttgataa
    13561 tagccagtaa ataaggaaga tcacttacat agtattgatc gtctaccggg atactcaaac
    13621 gatggatata ggtttggtca gcagttataa tgaccgctac actcgtagtt gcactacgcc
    13681 aaaacgaagg atctcctaac aaattttcaa ttttttcttg gaacggggac cagtcatgtt
    13741 cggaatattt tttctcaaaa cgtttctttg cttctttcgc aaaatttttg aatttgatct
    13801 gatctttttc cacatcttga tgggcgacat gcgtattcag catgatcgtg acgaatggtc
    13861 ctttagcgcc ttctgaagtc aattgtgaca ataattcttt atcctttttt gccataaacg
    13921 tacccctcct aaaaaaatca ttcctttgtt tctatctaaa gtctaccaca acactatttt
    13981 tccatacaat aaaacgtatc taaaaaaata aaacggtttc tacggtttta aatttagcta
    14041 tttttttaag ttatcttcat aatttcctca attatttagc cccagtcttc aactattctt
    14101 catgaaatgc tgataaacta agaaaaagaa taaaagaggt ggatcttatg catttgatta
    14161 tgattttcag tttattaagt attatcagtt tatttgcttt cagcgcttcc cgcttattag
    14221 atgcggaaac acagcctatt cctgtaaaag tacgtaaaaa cgaccgctaa ccttccataa
    14281 cgtgcgttct tatcctaaag ttttttcaga aacattgaaa ctctctcccc tctctgataa
    14341 aattttaatt tgtagcgaaa caaattaatt acaaggagat cggatatgtc cataaaaaaa
    14401 acacgtctag tcctattatt ggctagtctc attctttgcc ttgcttcttt tctaccagct
    14461 gttgctgtgc aagctgaaga tactttcaaa gtaaatgcaa aagcagcctt tgctgtagat
    14521 gcgcaaagcg gtaagattct ttatgatcaa gatggagaaa aaaccatggg gatcgcttca
    14581 atcacaaaaa tcatcggtct ttacatcgtc cttgatcaag taaaagaagg gaaactatca
    14641 tgggatgata aagtttcaat ttcagattat gcagaaaatt tgagtgtcac cccagattta
    14701 tcaaatgttc ctttgcataa agaaaacacc tacaccgtta aagaattatt cgactctgca
    14761 atcatccagt cagccaacgc ttcaatggtt gccttggcag aaaaaatctc tggcagcgaa
    14821 gcaaaattca ctgaacgaat gaaagaacaa ttgaaagatt ggggtatcaa agatgcaacg
    14881 atcgttaatg catcaggact gaataactct tatcttggcg aaaaccgacc agaaggtact
    14941 ggagaaaacg acgaaaatca aatgtctgct caagatgtgg ctatcgttgc ccgtcatttg
    15001 atcttggatt ttccagaaat actggatgta tctagcacta cgacacaaat gtttggcgaa
    15061 aacacgcaat caccagtaga aatggtcaac tggaactgga tgctcccagg attcgtgaat
    15121 tataaagaag gcgtggacgg attaaaaact ggtacgactg aacttgcagg agcctgtttc
    15181 gtcggtacga tcactaaaaa cgatcaacga atcatcactg tcgtattgaa tgcagacggc
    15241 catgcggaaa acccaagtgc ccgtttcaat gaaacagctc gcttaatgga ttattgctac
    15301 gacaattggt cagaaaaaga gcttggaaaa gccaatgcaa gtatccctag tctaaaaacc
    15361 attgatgtaa aagacggaaa agaatcaaca gtgcccgttg tgcttaaaag tcctgtaaaa
    15421 gtatgggtac ggaacgatat ggatactgga aaattaacaa tcacaccaac tcttgacaaa
    15481 aaacaagtca ctgacaatga gatccaagca cctatggata aaggaacaaa aatcggtacc
    15541 gcaacgatca ccttggcaga agataaatta ggttatcttg aagacaacaa cgcgccatcc
    15601 acagaaatca tcacggataa aaccgtagaa aaagcaaata ttttcgttat tggctggcga
    15661 cgcgtcgcag aatttttcgg taacttgttt taattgaaca aaaagaaaaa agcatctttc
    15721 catgtggaag atgttttttt gctttttcta gtttaaataa cagctttttt catctatttt
    15781 cgttatactg tataagaaaa aggagttgaa tttatgaatc taaccagaga tctgattttt
    15841 ttggttctct atcttattgg ggtattctcc atcgtttatt ggcgaaaaca gaaaaaggat
    15901 ccttccttgt tcttttacag cagtacatgg acgtttgcgg taatcttgac ttattttgtc
    15961 gtgttggaaa ttttttattc gaccttttat tttttgattc ccttagtgtt tttcagtatc
    16021 tttgcctttt cctatttttc agaaaagcga aggctgttga atggtctttt attcaatatt
    16081 ttcctcatta gttttggtat ctacttgttt gtcttgctat acgaaacaca aaatattttt
    16141 cttggcggac tgattgcctt gatcacgatc ccgttactgc ttgtctttct attcggcatt
    16201 tatggattga tcgtttttct tttttggaac ggtgtcacag ttttacgtag agagtcgcat
    16261 tcattagcta atcttttaac gttgatacta gccatttttc ttacattgtt tcttgttttt
    16321 gactttttct tgttaaaata tttaccacaa tggataaatg ctttgttttt ctgcatccca
    16381 ttgatcttga tctatctatt tatcgtattc tacaactttt tgactgtctc attcttgtat
    16441 cagttcaacc gccctcgata caaccaagac ttcatcgttg tgttaggcgc cgggttgatc
    16501 aatggagaaa cagtttctcc tcttcttgcc aagcgaatca ataaggcgat tgctttttat
    16561 tgggcacaaa gccgtgcgac attaaatccg cctatcctat taatgtcggg tggacaggga
    16621 gcagacgaaa aagtaccaga agcaatggcg atgaaacaat atgccatgga acaaggaatt
    16681 cctgaaagag atattcttgt cgaaaccaat tcgacaacca ctttggagaa tatgcgctat
    16741 tcaaaggaaa tcatggatca gcaaatgaaa ggtccctatc gcgcgatctt cagttccaac
    16801 aattaccaca ttttcagagc tggtctctat gcacgacaag cgaaactgaa ggcaaatggc
    16861 attggggcaa aaacagcttt ctattacttg cctaacgcgt ttttaagaga atacatcgcc
    16921 atcttgatga tgaacaaaaa aagacatatg atcatttgcg gattgctttt cgcaggttcc
    16981 gcattcttat cactgatcac tttacttttc taaaaaaaca aggtgtgaca aaacatatgt
    17041 cacaccttgt ttttttcttt cattctctta actattggca aaaagagtat cccttttctg
    17101 accgctactt gctgagcaga ataaataccg ctgttgcctg aacacataaa gctgatcaag
    17161 cagatcataa agaagtatac tgccgcttgt gaaccgaaca attcgatccc catcacaaag
    17221 caggcaatcg gtgtatttgt cgcaccagaa aatactccga taaagcccaa tcctgcaaga
    17281 aaaggaacag aaacatggag caaaggcgct agtgctgcac caagcgttgc accaatttca
    17341 aacaatggtg tcacttctcc cccttgatat cccgcgccta aagacaaaac tgtgaagaat
    17401 aacttcccaa taaaatcata gacatgtgcc tgtccattaa atgattcctg cagtaatgga
    17461 agactcaatc cgagataacg ttgtgtttgc aaaatcaatg ctgcggctac aactacagcc
    17521 ccacctatcg tatttcgcca aacaggatga ggaatccatt gtgcatatcg cgcttttaaa
    17581 aagacgatcg aacggctgaa tacccaacca atcaaaccaa aagcaatccc agcaacaaaa
    17641 actttgaaga acagcaacat actccattca ggaatcacac ccatttggta atgtagatga
    17701 gaaacaccat agctttctgt cataaaattt gcaaacagtg cagcaaagaa gctagggaag
    17761 atcgcttctg ttcggacttt cccgattgcc aacacttcta atccaaataa tgttccagct
    17821 aaaggtgtgc caaatacaga actaaaaccc gcactgattc cactgataat caagatttcc
    17881 cgttctgatt tatccaatcg gaacaaacgt gctgtattat ctgctaacgc tccccccatt
    17941 tggactgctg ttccttcacg gccaacagat cctccaaata aatgggtagt tatggttcca
    18001 aacagtgtaa gtggaataag tcgcaacggg attttttctt ctccaccatt tccttgatcg
    18061 atcactaagt tgtttccacg actagaaaga ccgccgtgat acgcatagag ataagcaaaa
    18121 agcgcaccac taactggtag tagataaagg agccaaggat gtgatagtcg gatatctgtc
    18181 acaactgtca ggctatttaa gaaaaaagca gataagctcc ccatgaatat accgagaatc
    18241 gctgtgatca ccagccactt cagcatgtac actaaaatgc gtactggact cggtaattgt
    18301 ttttccataa aaaattcctc ctcaataaa
//
LOCUS       DAYYAR010000049        18219 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000049 DAYYAR010000000
VERSION     DAYYAR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.049"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..636
                     /locus_tag="ACHAT0_001440"
     CDS             133..636
                     /locus_tag="ACHAT0_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8451389.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="ACHAT0_001441"
     CDS             763..1800
                     /locus_tag="ACHAT0_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFM8451390.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="ACHAT0_001442"
     CDS             1815..3512
                     /locus_tag="ACHAT0_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFM8451391.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="ACHAT0_001443"
     CDS             3505..3966
                     /locus_tag="ACHAT0_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFM8451392.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="ACHAT0_001444"
     CDS             3979..4839
                     /locus_tag="ACHAT0_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451393.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="ACHAT0_001445"
     CDS             4877..5266
                     /locus_tag="ACHAT0_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8451394.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5253..7700
                     /locus_tag="ACHAT0_001446"
     CDS             5253..7700
                     /locus_tag="ACHAT0_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8451395.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="ACHAT0_001447"
     CDS             7705..9813
                     /locus_tag="ACHAT0_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8451396.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="ACHAT0_001448"
     CDS             9810..10814
                     /locus_tag="ACHAT0_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8451397.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="ACHAT0_001449"
     CDS             10833..11744
                     /locus_tag="ACHAT0_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8451398.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="ACHAT0_001450"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="ACHAT0_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12406..12621)
                     /locus_tag="ACHAT0_001451"
     CDS             complement(12406..12621)
                     /locus_tag="ACHAT0_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451399.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13089)
                     /locus_tag="ACHAT0_001452"
                     /pseudo
     CDS             complement(12880..13089)
                     /locus_tag="ACHAT0_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(13392..14831)
                     /locus_tag="ACHAT0_001453"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="ACHAT0_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="ACHAT0_001454"
     CDS             complement(15379..16050)
                     /locus_tag="ACHAT0_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8451400.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="ACHAT0_001455"
     CDS             complement(16399..17850)
                     /locus_tag="ACHAT0_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8451401.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(17962..>18219)
                     /locus_tag="ACHAT0_001456"
     CDS             complement(17962..>18219)
                     /locus_tag="ACHAT0_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HFM8451402.1"
                     /translation="IRPVFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFS
                     LAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         5940 a         3055 c         3502 g         5722 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgatttacc tctctttttt ctctttaata
    17881 gtgttttcta ttaaaaatta tgataaatat tttcaatgaa ttgagtaata cagtatattt
    17941 cttgatttca tttagtaacg attatgtttc aatgtttaat cctgttactg cctttaaaat
    18001 aaaattatag ggttggctaa aactaggatg aaataaataa tctaacattt tcaatttgtc
    18061 taatgtaatt tgttcttcta tagcaagaga aaacattgat aatagtccag ataaatcttc
    18121 ttgactacaa agttgtacac ctactattct ctttgtattt ttttcaaagc taattcttaa
    18181 ttttacacga ctattttttc tcaaaaaaac aggtctaat
//
LOCUS       DAYYAR010000050        17340 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000050 DAYYAR010000000
VERSION     DAYYAR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.050"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..594)
                     /locus_tag="ACHAT0_001457"
     CDS             complement(49..594)
                     /locus_tag="ACHAT0_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8451403.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(606..1298)
                     /locus_tag="ACHAT0_001458"
     CDS             complement(606..1298)
                     /locus_tag="ACHAT0_001458"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8451404.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1317..1601)
                     /gene="macP"
                     /locus_tag="ACHAT0_001459"
     CDS             complement(1317..1601)
                     /gene="macP"
                     /locus_tag="ACHAT0_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8451405.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1614..2177)
                     /locus_tag="ACHAT0_001460"
     CDS             complement(1614..2177)
                     /locus_tag="ACHAT0_001460"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8451406.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2405..3067
                     /locus_tag="ACHAT0_001461"
     CDS             2405..3067
                     /locus_tag="ACHAT0_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8451407.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3191..4387
                     /locus_tag="ACHAT0_001462"
     CDS             3191..4387
                     /locus_tag="ACHAT0_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8451408.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4488..5864)
                     /locus_tag="ACHAT0_001463"
     CDS             complement(4488..5864)
                     /locus_tag="ACHAT0_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8451409.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5901..6767)
                     /locus_tag="ACHAT0_001464"
     CDS             complement(5901..6767)
                     /locus_tag="ACHAT0_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8451410.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6856..7881)
                     /locus_tag="ACHAT0_001465"
     CDS             complement(6856..7881)
                     /locus_tag="ACHAT0_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8451411.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7899..8573)
                     /locus_tag="ACHAT0_001466"
     CDS             complement(7899..8573)
                     /locus_tag="ACHAT0_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8451412.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8757..9650)
                     /locus_tag="ACHAT0_001467"
     CDS             complement(8757..9650)
                     /locus_tag="ACHAT0_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8451413.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9724..11421)
                     /locus_tag="ACHAT0_001468"
     CDS             complement(9724..11421)
                     /locus_tag="ACHAT0_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8451414.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11414..12727)
                     /locus_tag="ACHAT0_001469"
     CDS             complement(11414..12727)
                     /locus_tag="ACHAT0_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8451415.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12727..16668)
                     /locus_tag="ACHAT0_001470"
     CDS             complement(12727..16668)
                     /locus_tag="ACHAT0_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8451416.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16777..17235)
                     /locus_tag="ACHAT0_001471"
     CDS             complement(16777..17235)
                     /locus_tag="ACHAT0_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8451417.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4859 a         3895 c         3012 g         5574 t
ORIGIN      
        1 ttcttttatt attttgtgct ttttatttta tcaaatcact ttgttttttt ataaaatttc
       61 tgcgcctagt gtggactcaa aatgtctcaa tgcccattca tgtccaacgg gatcgaaact
      121 tgccacggaa tctttgaaaa tatggagttt gtatcctaga ttatacgcat cgactgctgt
      181 atgcaacaca cagatatctg tacatacgcc ggtcaaataa atatctgtga tctggcgttc
      241 tcgcaaacgg atgtctagat ctgttccact aaaagcggaa taatgccgtt tgtctatcca
      301 ataaacattt tcctcttgct catagtcttg gtataaactt tttaaagagc cgtacaattc
      361 ccgtccaccc gttcctatca cattatgcgg aggaaataag cggttctccg gatgaaagga
      421 atcttttgga tcatgcgcat caatggcaaa aacgacaaaa tccttttgat caataaactt
      481 ctttgtatga gcagtcagct ttttttcaat tacttgtcct gctgtcccag tagtcagctt
      541 tccatcctct gcaacaaaat catatgtgta gtcaatcgag atcaatgcgt tcatcttagt
      601 cctccttata cgagatgttt gacaaattcg atcaccattt ctgctgatcg tttgcctgcg
      661 tcttcgataa attcgtcaaa gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca
      721 cgtacgatca aaaatgggat attgaactga cgagctgttt gtccaacagc ggctccttcc
      781 atttcgcaag ctaatgcttc agggaaattc gttaagattt ctttgatttt atctggcgag
      841 tcgacaaatg tatcacctgt aacgatcaat ccttcttttg cgttcaaatt cgttgctttt
      901 gctgctttaa ccatttcact gcgcaaatat gtgcttgctt cataatatag aggcattcct
      961 ggtaattgtc ctgggttgta gccaaatcct gttgcatctg catcaaaata agctactttg
     1021 tctgatataa cgatgtcacc tacttgtaag ccttcaccga ttccgccagc tgaacctgta
     1081 ttgatcacca tgttcacgcc gtattgttgg atcaacagac ttgtggtaat agatgctaag
     1141 acttttccta taccggaacg tacgacgatc acttcgtggt tacctaatga acctgaaata
     1201 aataaagcgc ctgcgcgttc ccatgataat ggttcgctta ggttctctct caaaattttt
     1261 acttcttctt ccattgcgcc gataataccg attttcatct ttttaattcc ttctttctat
     1321 ataaatgcga ttgctaaaaa aaccacacat aacattagga taacaataat aagagatttc
     1381 atcaaaaaag agttccattt ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt
     1441 ggtttattct ttttgctttc tttacgataa aagctggcaa tctttttttc ttcttgctga
     1501 taagctgttt ctgcttttcg ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc
     1561 ttgcgaagtt cgcttcgcgt aataagcggt cctttggcca taagccgatc cctctacttt
     1621 cctttggtca atagttccca gtattgaatc gcataaatcg tctttgcatc acaaatcaac
     1681 tgatctttca tcgcttgttg cgcctcttca agagttagat gatacaactc cagtacttcg
     1741 tcttcatctt gtgctaaagg attctcgacc ttctctaccc cttgtgcatg ataaatatga
     1801 aggacttcat ttgcaaaacc tggtgaaaga tacatcgaag taagatggct cagcgacttc
     1861 gcacgatagc cagtttcttc ttctaattct ctagctgctg tcatttcggg attttgtcct
     1921 tctcctggat cgatttttcc agctggaatc tccaaaatca ctttttctaa aggttttcga
     1981 aattgtttga caagaagcag acgatcttgt tcgtcaaaag caatgatccc gacaccgcca
     2041 ggatgaaata ccagttctct cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca
     2101 gcaacatcaa taatttttcc gctatagatt tctttgcgac tgattgtttt ttcttcaaat
     2161 tgctcattgt tcagcatatc ttctcctgct ttctttgtaa tcataactgc ttttttttca
     2221 tttttcaatt tttctcaaaa actccttatt agtttaccat agataaacct tagattttct
     2281 ctaaatatag aaaatccttc tttagtccga tactctttct agtagtccgt atgctaaaat
     2341 aagtatgtaa tcgaataacg aaaagaattc aatactcaac gataaatgac atggagggtt
     2401 tattatgata aaacgaaatt ggccgtggct tctagcattg gtcttgctat ttctctttgt
     2461 ccgaaacaat agtttttctt ttgttctagg acttttattg ataggtgtag ttttagtttt
     2521 gtggggactc tttggttcgc gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac
     2581 tgacgaactt gaaactcact atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt
     2641 tcgacaaaca atgaatcaaa caaaaaatga gatcaatcaa ttgcaaatga atatgcagca
     2701 gacagccaaa cttaaagcaa tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt
     2761 attcaaagaa ttggtgaaag aaccaaaccg actgcatgaa gcaagtcagt ttctttatac
     2821 gcatctgccg aatctagttg atttgacaaa taagtataat gagatcaatg accatgagat
     2881 caaaaacaaa cagacctatg aaaaactaga agaaagcgcg cagatcatcg accagctgtc
     2941 agctttaatc gcacaagatt atcaaaaatt cgttgctgag gatctagatg atatcgacgt
     3001 agagatatct gttgcgaaac agagcttaaa gcgtgataat aaatatgaac cagaacaaaa
     3061 ggattaactc ttttgttcat aaaagaagac tcggaacatt cagatgatgt ctggctgttt
     3121 cgagtacttt ttctaaagta gagacattca tgtaagaatc gagaacagat tcaacaagga
     3181 ggaatttttt atggatcata aaccgactga aacaaaagat gttacaccag ttaacgatac
     3241 acttgaagat ttattgaaca acccgttttc aactccaaca gatacgctga ccacttcaca
     3301 acaagcggag attgatcagc tccaagagca acagacagct gctcgtttaa ttgacaaact
     3361 accggcggaa cgccaagaac aagctaaaca gctagcagca aaaatcgatg ctaatgatgc
     3421 acagtctgtc atcagctacg gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc
     3481 gatgttgaat catgtgcaag cacaagatat tggtcctgta ggagattcct taactgaact
     3541 gatgtatcgt ctccaagaag ccaatcctga cgaactgcgt gccggagaag ggaacatctt
     3601 ccagcgtgtc ttcgggaagg tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa
     3661 gattggtgca caaattgaca agatttcagt gaaattagat aaagaaaaag acggtctgct
     3721 aaaagataat ctgatgttag aacagcttta ccagaaaaac aaagactact ttgatgcact
     3781 gaatatttat atcgctgctg gagaattgaa aatggaagaa cttcaaacaa cgatcattcc
     3841 agaagcgatg aaaagagcag aagaaactgg cgatcagatg gatgttcaga ttgccaatga
     3901 ttacacgcaa ttcctagatc gtctggataa acgtacgcat gatcttcgct tagctagaca
     3961 gatcacgatc cagcaagcac cacaaatccg tttgatccaa aacacaaatc aggccttggc
     4021 tgaaaagatc caagcatcta tcgctacagc cattccgctg tggaaaaacc aagtcgtgat
     4081 cgcattgacc ttgcttcgtc aaaaagatgc agtaactgct caacgccaag tctctgaaac
     4141 aacaaacgac ttattgaaga aaaattcaga aatgctgaaa atctcagcca tcgaaacagc
     4201 gaaagaaaat gaacgtggaa tcgtggatat cgagaccctt caaaccactc aaaatgattt
     4261 gatcgaaaca atccaagaga cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca
     4321 tgcagaagta gagcttggac acatggaaga agatctgaaa caaaaactgt tggatttaac
     4381 ccaatagatc acctttcgct tgttactaaa acaaacgccc aacaacagtt tattacaact
     4441 gttgatgggc gtttttctta tctttcttct aacttctcca ttggagtcta tttctcattc
     4501 atcttgctgt gtccatagcc atagccaaaa taaatgatca gtccaataac cattacgata
     4561 ccgaacacga tccatgtcac agtttgcaga cgaagcatca atacaataca agtcaaaatg
     4621 gagacgattg gcaatagcgg aacgaatggc actttgaatt ctccagcttt tggtttgcca
     4681 aacatttttc tcaacttcag taagcctaga ctaacaagca tcaaataggc gagcgccatg
     4741 atattcgtta gttccgctaa tacattcaat gggaaaaatc cggcaaagaa tacagctgcc
     4801 acaccagcgg cataagtcgc ttttttcggt gtacgatgct tttgatcgat ttctgtcata
     4861 aacttaggaa gtaagccatc cttgctgaca gcaaacagta aacgagcaag tgaatacatc
     4921 atcgagatcg ttaccgtcag tagcgttaaa attgccccta cagcgatcac cgcgcccact
     4981 gttccatgtc ctacatacct cattgcaaaa gccactgggt cattgactcc tagttcagta
     5041 tacggaacga ttcctgtcaa gatcagcgtc acgatgatgt aaaggacagt agcaatcagt
     5101 atcgaaccaa tgattcctct tggcacatct ttttgcgggt ttttgacttc ctcggctgcc
     5161 attgcaacgg catcaaatcc caagaaagca aaaaacacaa gagctgcacc atctagcaca
     5221 ccggaaaaac caaaaggcat gaatggctgc cagttatcag gtttaacgaa aaatattcct
     5281 acaatgataa acaacgcgat gataccaaat ttgacatata ccatggcatt attcaaacgt
     5341 aatgctttct tggcttcaag cgacacgatg taagtgacga atatcactac gagcaccgca
     5401 atcaagtcaa tgtatgtacc attttccgga ttgtacccgc cagttaatgc ttgtggaagt
     5461 tcggccccta aacttttcaa aaaaccttgc atatagccag accatccgga agcaacagag
     5521 gaaaccgcta gtaaaaattc cccgatcaac aaccaccctg tcagccaggc agcaaattca
     5581 ccaaatacaa catacagata ggcatatggc ccaccaatca ctggcactcg cgaagcaaat
     5641 tctgcgaaac ttagtccaga caaaatgaca acgattgcag ctaagacaaa tgaaagtgac
     5701 agtgctggac cagcattttg attggccgca acccctgtca ccacaaatat tcccgtaccg
     5761 atgatcgcac caattccaag catgatcaaa tcagacgttt ttagttcttt tttcatctca
     5821 ctcgtatgat tgagattgat ctctttctta cgaaacaaat ccattgaagt tcctccccat
     5881 tttaggttgg aaaacagcga ttaaactgtt ttcatatacc ctaaaaggat accacctaaa
     5941 atgactaaaa ggcaaccaac aataacataa atgagctctt ttttcgtttt tctttcgcca
     6001 aggatgaaaa tcccacctaa agtagagata atgatcccca tttgtgaaag tgagaagcca
     6061 acagctaaac caagtgattt gactgttaga agcatgcaga tgtttcctaa gccccataat
     6121 aatccacaga tcatgttttt ccacacaaac tgatcgactt taactttacg gaaagcaaag
     6181 aagcttgcgc ctaagatcat tccgatactt tgaggtaaga taattgacat cgcatccaaa
     6241 ttcgcccagt tgacgatgat ggtatataca ccataaccaa tcgtagagaa gatcaatgct
     6301 ctaaaaccct taccaaaatc aagcatttta ttatctgtat ctatttttcc ttcgtcatct
     6361 tgtcgggcag tcaaatacgc accgcttaca agaagagcta aagcaataat accgtataca
     6421 taatctttcg tttgtgtcca ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt
     6481 aattgaaaac cagtagacaa tggtagccct acagaaacac ccatgtactt cattccatgg
     6541 aactgtccat tttgtccaac actccaagct aagccggata aaaatccgat caagaaaatc
     6601 caaggggtga ttacaggact aacaacgaaa aagacaacta aagaaaacag aaatgcaccg
     6661 atcgtcattc ctagtgtttg ttgattggca tcgccaccaa ttttaccact tactaaacca
     6721 atactacccc atgccaacat agggactaat gctattaaaa tttccataac caatctcctt
     6781 ttatttcaca tgataagaaa cttatcagcg aaaacgttaa caacacaagc actttttttc
     6841 gtttctaacg attttctaac gtttttcatt ataaaaattt aagattggga aaaataaagt
     6901 cattgttgtc ccttttcctt cagttgattc agccgtcaaa tggataccta atttttcggc
     6961 cagatttttt gctaaataaa gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt
     7021 tctcccagta aatcccttat caaagatccg tttcaagtct tccgctggga taccgatccc
     7081 tgaatcttta actgaaaggc tgactccttg cgcttccttt tctatgctca caatgatgtt
     7141 tccgtgatcc ggcgtatatt tgatggcatt actaagcaat tggttgaaga taaaagcgat
     7201 ccatttagca tcggtcaaaa cagtatgatc ctcaccaacg atctcgtagc gaatattttt
     7261 ttggataaaa taatttgcct gactgcgtat cacaggctga atgattcctt ttagtccata
     7321 ttcttggatg aggtagtccc gcgagaaact gtcgagtctt gcataataca aaacttgttc
     7381 tacgtattca tcgatttttg ataattcatt ttctagtaat gtatactttt gatcgtcaat
     7441 atcgaactcg atcgaacgaa ctagcaattt agaagcggaa agtggcactt tgatttcatg
     7501 gacccatgag tcaatataat ctttttggtc ttgttgatta ctgattgctg attgcattac
     7561 ttcttggtgt tcacggatca gctgattgat atattcttct accagttttt cttcttctgt
     7621 cctcccacca ctcaaataat tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc
     7681 ccaccatctt ctcttgctca agtaaccacc cagcagataa aaaatcagaa agacgccctc
     7741 aatcaaagct aaataagcaa ttgtgctcaa atcaactttg atgtttggcg ccagccacat
     7801 cacaaagatc gtcaatgcaa taaaaaacag ccatcccaag atcaatgtcc actgatcttt
     7861 caaatattta aaaaaattca tcttaaccgc tcatcctttc agggaacgat atatccctgt
     7921 cctacttttg tttcgatata tccttggata cctgcctgtt cgatttttcg gcgtaaccga
     7981 ttgatgttga ctgtcaatgt attgtcatct acaaatcgtt cgtcatccca cagtgctcgc
     8041 agcaattttt cccgacttaa gatctttcca ttgtgcttca tcaaaataaa taatagacga
     8101 tattcatttt tgctcaaatc aatgatctct ccgttgattt ccatacttcc gttgtcaaca
     8161 ttcaacgtaa tgccattgtg gctcatcatt tcactagata attctgtgta gttgtaagaa
     8221 cggcgaagta atgcattgat ttttgcaatc agtacatcga tttggaaatg ttttgtcaca
     8281 aaatcatctg cccccatgtt catagccatg atcatatcca tattcgtatt cctgctggag
     8341 ataaaaatga tcggtacttt cgatacttca cgtatttttt gacaccaata atagccatcc
     8401 aaaacgggaa gattaatatc tagtaataca agctgtggtt cttctctttc aaagtctgag
     8461 aacacttgat tgaaatcggt cgttccaaag gtttcaaatt gccatttctc aagttcctcc
     8521 atgatcaatt gtcgaatgat ctcttcgtct tcgactacca tgatttttgc cataaaaaat
     8581 ccctccatct ttcttcttgc tactacaata tcaaagaagc ccttgtgaaa caagttggct
     8641 tacctggaaa atgtcatttt agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg
     8701 cgtttgtctc ttccaagcag cttaaattta ttatgttatt cttaataatt ggtctattat
     8761 ctgttatttt tctttcgttg tttttgtctt ttaaattttc gataacacca ataaccgaaa
     8821 agcgttagca ctagcaatat acctagaagg agtataagta atcgataaag atggtatttt
     8881 tttgtctgtt ctatctttga agagacttct tctgtaaagg gaactcgtct tcctgtcact
     8941 agtaatcggt gagtgttcac ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc
     9001 ttttctctaa ttgctaatgt atttgttttt gttggaagga caacaatttt ctcaacgact
     9061 tcataagcga gcatccgacc atacacttca agatagaatc ggtcatcttg ctccatttct
     9121 tcaagatccg taaagagttt tttctcagca ataccgctat gggcggtaat cacggaatgt
     9181 gtactctctc ctccagttgg aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac
     9241 aaaagatcat tcgtttcatc aaataaaggc aagctaacat tgattttcgg aatatagatg
     9301 gctccgatca tatgttgttc gtaatattct tttcctggat tttggacatt tctaaccgct
     9361 tgctcaaaag gatctttgac ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc
     9421 tgatttttct tttcatgcac tgctaattgt tgctcatgtt tttcttcttt atcgcgttca
     9481 agctgctttt gataattctc gatccttctt tgatccaaat aattgctgag agcatctgct
     9541 aaaaatggat atgtaaaaac tgcagctccg gcaagaaaca tcactgccat aaataattgt
     9601 aataaaagac tgatttgggt ttgaggtcta ttacctattt tctttttcat tttcttcacc
     9661 ttcttattta aaaaaactgc agcaaccttt ataaaaccga ttgctgcagc ttatttttgt
     9721 tgcttatact tcagacttca ttttagattt acggtaccag ctgtacgctc caatcatcaa
     9781 acttattccg attagtaaga atgccaagag cccgttccca ccagtagcag gaagtgaccc
     9841 gcgtttcata ttcggtactc tttctggccc tggattttct actcctacga tctgaatttc
     9901 ttcattatag cttccataag agatgataaa ttcagtaaag gctgcctcac ctgttggaag
     9961 tacataacct tctggcgcca tggtctcttt catttgatag gttccataat ctaacccttg
    10021 aacctcaaag tatccatttc catctgcgac aaattctgtt gcttgtccgc ttgttgccca
    10081 ctcaattcta tctgctcctg attcataaac agctaaaggc gactcccctt tataaaatat
    10141 tgcgtattca gaacggtctt cattccacaa ttcgaatctt gcatctttaa gcaattcact
    10201 gcttgatgca tcatattttt caaactttct tccacctgtt gtgactttct ctggcggctc
    10261 tactccttct tcataatcaa attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt
    10321 atggaaatca tccgcttcta gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc
    10381 tcctccacgt gcatctgcta atctacctaa agtatctact ttggctactc cattttcttc
    10441 tgtcaaaatc gtcacgacaa acccgttgct tactacttct actatataat cttgtccttt
    10501 tgttaaaatc gtctcgccgg ctcgcacttc tatttcctga tcataataag ttagaccaac
    10561 tgtaggcgta tctgtgattt tgtaattttt gataacagcg cttccgtttt caaggacaga
    10621 atcgatgaaa taaggaatag gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag
    10681 atcaatataa gaaatcgttt cttgttctcc agcttcatta ataatcgtta cttgtctaag
    10741 gtcgctttcc tctacattca tctgtttatc tgcttctctg atttcgtttt tcggatacaa
    10801 atgcacatct gtattgtatg tgtctttcca tgtagaacca tcccacacgc catccaccat
    10861 atcaggcatc atcactggca tcataacaac cattggagac gcgattctgg aaatatgagc
    10921 tggcgaataa gtttctaaga aaagatagac tttatctcta gaagaaggtg cttcttccgt
    10981 cacttggacg gtagagaata ccgcttctcc agcttcattc gttgtttgtt tgtcaatact
    11041 tgtcagtcga tattttaaaa accaactttc tgtgtgccgt ttttgaatcc attcaatcgc
    11101 ttcggcagaa ttaagaccgt cttctcgttc cggtgttaaa ggattatctc gaatcaaatc
    11161 gtaatatact tccgttacgt caaacaaatc aaaatcgact cctggaatat tttcttcacc
    11221 aaaatccgta gaaaccaatc cgctattcgg tcgttcttcc ggaacagatg agaaaccttt
    11281 tttgtgtaat gtaatcgtca cttcacttgg tttttgtgcg acttcttcgg ctttgactag
    11341 ttttccgttc ccgaaaatcc ctattcccgc agtaacgagt aacagtacac tcattcccgt
    11401 gacaatcatt tttttattca tttttttctc cccgttcttt ttttattaat aaaacagcac
    11461 ctaacaataa aagaattcct agtaaagaaa tcaacgttct tgcttcattc gtttgcggga
    11521 atacggacca ttttcctgat tgacctggat tgcttggacc actcggctct ttcggtttgt
    11581 ccgttgtttc ttttacggtc tcgttataaa cacgtggtgt tgcggattca taggcagaga
    11641 ctggaacttt cccggattct aattcagcca tctcctgtcc gttaactact acatattgtg
    11701 gttggccttt tgcatccacc caaaattcag ggatgatgac ctcgattctc ttacttgctt
    11761 catccatctc gtaaccttcc acacctttta attcttcaaa ataataggtg ccagaaggca
    11821 agaaacgttg tcctgtcgta acaaggccct ctttatccga aatgaatttg cttacatttt
    11881 tgtcattcaa aggatcattg tctgctggtt tgatccattg atttttcaag tcattagcag
    11941 gagccatatc taaataatat ttcccccctt ctatcatctg atatagagca aaaacagcac
    12001 cttctaatgg ttttcctttt tcagttgttc ctctttgacg tccatacttg aagaagtatg
    12061 gatctcttag ataaccgaca tttttaggat agatatgaat ctcttgcagt tcctcggttg
    12121 attctgtcgg gtgataaatt ggcaaaatag cagcaattgg gatagctgtc tgatcgatat
    12181 caacatccaa tccaacttct ggatcaatcg attcttcgaa aatcaaataa actgcatccc
    12241 gatcttggtt tttcttaggt gctttgatac gtgcaatccc gtctcctaat ccgccaaccg
    12301 tatctttccc agttgttacc gaagtcccta tttggacaag cctttcttct gtgattagct
    12361 gacggatatt gtttctgttc gtttcagcga tttgctggac gaattcttta ggagtcatgt
    12421 tttcagcagc ttttttttca tgataataat cggtcatgtc gtaaatggca aatgtcgctc
    12481 catttagtgg caccgtctgt gaaatgatat ctgctgtttc ccctgcttcc tcagagatag
    12541 ccaagccatc attttcatgg tattcaaatt gatcgttata atcgatatct cgaatcatcc
    12601 gtttatgcaa cacgaattcc acgacatctt cccctgattc ttctgccgac atcgcatgtg
    12661 gaagaaggac tgctgcaagc ggtagtaaaa caagtaattg aagcatcaaa cacaacagct
    12721 ttttcattac cttgctccct cctttttaga ttgtctgaca aacaaaccat aggctgctcc
    12781 tgcaagcgcc atgaagaatg cagcaagcag gatatagaat aatcgtccgg atcctcctgt
    12841 agcaggcaac ggattttcag gatcgttccc gacactaagc tgtaatctga ttcgatttgt
    12901 ctccaaaagt tcatattcta actcttgctg tagatcaggc gtctctgctt cttcgtttgg
    12961 ataatacgaa atctcaactt ttagtgtacc cgcttctttt cctggaacta cccgatcatt
    13021 ttctatgtca ggttcttctc gataactaat ttctaaaata aagtaacctg gaagttttct
    13081 atagctgtca ggtgacttca cttccttgat ccgatagacc ccttccaaca agtctgaagc
    13141 attgaatagg gaagaatcgt ttggatctgg tgccaagcct ccctcgatga tttcatactc
    13201 tcctgcacta ttttgtttct caattgaaaa ttctgcgcct tcaagacgtc ggctattcat
    13261 actgtcaatt ttcaaaagtt ccaaatcgat tggcgctagt tcatcttcta aggttacttg
    13321 tagtacacca tcgatgattt catagccttc cggcaaggga gcagtttcgt cttcgatgac
    13381 cgtatcttca atgataaact taccttcttc attcagtgca aactggaaca cttgatcaga
    13441 tagccgatac ccatttggtg cgacagattc cgagacctca tattcaccaa tagacagatg
    13501 cagaggttgg tcgtaattta aatcgacata cttttctact cctacggcac gagaatagaa
    13561 gtatccgatc ccaggcaaac gtttcgattc ttctggttct aacggattac cagtgtatgc
    13621 tcgatacagc gggaaggaag aatcccccac tttcctgata tcgaaacctg caccatctaa
    13681 tgtatggcct tcatggtcta attttctaac tcggatctcc cgataatcaa acgtattgta
    13741 agcagtgaat gctacccata aacgatcctc ttcatctgtt gtatacgtga gatattgcca
    13801 attttcttca cctgtttgct tgagttggat agtccctata gcattatcgt aaagtcgcaa
    13861 gatccatgtg tcattttcat caggttcttt gaaaccttct gggttctggc tctcaatgat
    13921 tttgtattct gcatagttgg tagtttcttc actatcctta taaacaaatg aatctggctc
    13981 tccgattgga tgaataactt tctcaccatc cacgattaca tatttcccgt cttcatctac
    14041 tgaatagaac gtgagtctcc ctttctcttc ctctacttcg ttcacaccag aaagcccttt
    14101 cgctactgga tatccgattt ccagtccgtt accatctgtt tcataaagtt caaactctgc
    14161 gccattgatt ggcatattac tgccttcaat tagtttattg atattcatgt ggattggctt
    14221 gaattcattt cgcatttcca gtttgacata caactcatca tactctgtgc cttctatgtt
    14281 aggctcttgt acaacagaaa gcttgtgata ttctccttca taataggaat tcggatcttt
    14341 accttccggt ccgccgactc tcgaaccaga tgtatattca tctacaagct cgtagtgacc
    14401 taacatctca tcttcgttgg ctgcatattt tttgatttgg aattgatacg tctcatctac
    14461 taactgataa cgtggtggtc cagcgatctc tctgatccaa aatatgtcgc tgactgctcc
    14521 agtttcatct cctaaaatcg gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt
    14581 tttacttgtt tcgattggtt cggcttcccc actctctgga ttttggtcat ataatgcaaa
    14641 ttcgactcca gccaacggct cgccattttg acccttcttg ctgacttgga aattacggcc
    14701 tttatgttcg taacgaatgg aagaagatgg gaaatacatc cgtccagtag tgatatcatt
    14761 ctcataatcc tccaggttct gaatataggc catattgttc actgtgcaaa aatttccgtc
    14821 ttgagctccc gcatcaagtt ttacttggta cttgatcctg accatttccc cttcacctaa
    14881 agaaatcgga gaaaatttat aagcttggtt ttcccgtccg acatacgttg tcggagtcgc
    14941 cactacttct ggtggggctt gtccatgacg ttcttcatcc catatttctg gctcttggta
    15001 cttcatgccg ttttctcctg gatctagatg gtaaaactga atatctgccg atctgccagc
    15061 tccaaatggt gagacattaa aaccagtttc atcataaagt gtggcttgct ggatcgttct
    15121 cttgaaggta gaagagatat cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt
    15181 gtaatatcgc ggtgttacgc catcttcttc taaatcagca atatttttca atacatttcg
    15241 tccaatagca tctagtcgtt gtctcccgct aacgtttcca tcgattccta aaccaatgga
    15301 gaagatactt ggcgggttct cttgatactt gtttttcagc caatgatgat aagcaactgt
    15361 gttgatcgca gatgactttt cttgcgaagt aggactgcca ttcgtattgt tattagaaga
    15421 accatcacca acccagtgag tcgctttatt atctccttct tttacctctc cataacgata
    15481 gtgtacattt acttcatctt ctggacggtt aaagtcagat gcataggtaa ctggatgagt
    15541 aaaccctgca ttagaagtat tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt
    15601 atcttcagca atcttatctt ccatcacagt ccagttgctc caagctccat tagtgctcgt
    15661 acgataactt gactgaatcg gtgcataact gaaggtcggt gtcccatctc ccacaacgat
    15721 taggattttt tctggatttc tattttctcc gccattgtct ttatacaatg tttcatagcc
    15781 atttttaagc cctagtgtaa gcggagttcc tccgattggt gtacgattta atgctgtatt
    15841 ctcacttccc gtcaaacgat tgatatcatt tgtcaatgga aagatgtccg aactgatttg
    15901 ctcatggtta ttgatagctg tactattatg gtaaaaattc accataccga ttcgaatatc
    15961 cagactcggg tcagagagca gatctttact catttcgttt actgcctcaa tcaacgcagc
    16021 atttttagtt tgactattgg tcccagcggt taattcactc atactcgctg acttatctag
    16081 aacaaaaacg atatcgattg gactgatttc ctttttctcg cccccgatga catctagata
    16141 gacattgtaa agggttgttg catcttcagg atgtagatca gatgctgtgc tgcttggcag
    16201 aacaggttct acccattttt tgatgtacgc cccgttataa tccaagtaac tgttttcaaa
    16261 attccgttca tttccccaaa gctgaacggt ttgagctgga acgtttctat cttcttcgcc
    16321 tgcagttgat ccattattag tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt
    16381 ataatcacta tcatcgatca agttctctcc gtcatatggc gagcctttgt ctccttgata
    16441 gcgttcattg ctttgtgtcc aatttcctaa cacttttgta tagtcattcg ttgtaaactt
    16501 cgggtagcga tgctgttcgt tttcaacttc tccgctagga tagatatatt cataggggtc
    16561 ttgcgtagct gttggcccaa gacgctccaa aataccgttg aataactggc gttgaatgcg
    16621 ctggagtgct tgaccgctgg gtacgattgg ttcgtccagc ttttccatct cattttcttc
    16681 gctacgttga atttcagcgg aatcttctgt atcagtttca ttttcttgta attcttggtt
    16741 atctattctt tcatcaacca ccacttgaag aggtagttac ccttcgttca catcggggat
    16801 cttttctgtt tcaaaaatca atgtgccggt ttctgcttct tttgaataaa cttttccgac
    16861 ataccattct tgaccatcga tcgtctcgac ttttaggtct tctccatctt catcatagga
    16921 ggatagacct gttccacgaa cgttttttac ggtaccaatc ccggcggctg ccgcatcgat
    16981 tgctaaacgt attctacctt cattggcact ttcctttttt tcaaaagcca gatgccattc
    17041 aagggtttct tctttttcaa tatacttccc ttcaattttt gcatagtcct catctaagaa
    17101 tacctcatcc tcctttgttt ctgttgactc agcagctaca tccagggtcg tcatatgata
    17161 gactgactgg aaatatggtc caaatagcaa actgacagtt actaactgca aaagcttcaa
    17221 ttttaaccct ttcacatcct tatactcctt tcaaaaatat ttgtcttctt tcacaacaat
    17281 caacaaaaca atgcccatct aattataatt taatttaatt ttaattttaa atcaaatgat
//
LOCUS       DAYYAR010000051        16275 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000051 DAYYAR010000000
VERSION     DAYYAR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.051"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHAT0_001472"
     CDS             complement(<1..1835)
                     /locus_tag="ACHAT0_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8451418.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHAT0_001473"
     CDS             complement(1832..2536)
                     /locus_tag="ACHAT0_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8451419.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHAT0_001474"
     CDS             complement(2548..4857)
                     /locus_tag="ACHAT0_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8451420.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHAT0_001475"
     CDS             complement(5052..5516)
                     /locus_tag="ACHAT0_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451421.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHAT0_001476"
     CDS             complement(5516..6142)
                     /locus_tag="ACHAT0_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8451422.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHAT0_001477"
     CDS             complement(6149..6514)
                     /locus_tag="ACHAT0_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8451423.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHAT0_001478"
     CDS             complement(6504..6842)
                     /locus_tag="ACHAT0_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451424.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHAT0_001479"
     CDS             complement(6832..7158)
                     /locus_tag="ACHAT0_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8451425.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHAT0_001480"
     CDS             complement(7139..7417)
                     /locus_tag="ACHAT0_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8451426.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHAT0_001481"
     CDS             complement(7419..8783)
                     /locus_tag="ACHAT0_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8451427.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHAT0_001482"
     CDS             complement(8796..9365)
                     /locus_tag="ACHAT0_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM8451428.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHAT0_001483"
     CDS             complement(9337..10524)
                     /locus_tag="ACHAT0_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8451429.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHAT0_001484"
     CDS             complement(10588..12315)
                     /locus_tag="ACHAT0_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8451430.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHAT0_001485"
     CDS             complement(12312..12764)
                     /locus_tag="ACHAT0_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8451431.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHAT0_001486"
     CDS             complement(12876..13256)
                     /locus_tag="ACHAT0_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8451432.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHAT0_001487"
     CDS             complement(13253..13639)
                     /locus_tag="ACHAT0_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM8451433.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHAT0_001488"
     CDS             complement(13641..13919)
                     /locus_tag="ACHAT0_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451434.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACHAT0_001489"
     CDS             13967..14695
                     /locus_tag="ACHAT0_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM8451435.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACHAT0_001490"
     CDS             complement(14841..15317)
                     /locus_tag="ACHAT0_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8451436.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACHAT0_001491"
     CDS             complement(15277..15633)
                     /locus_tag="ACHAT0_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451437.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACHAT0_001492"
     CDS             complement(15611..15796)
                     /locus_tag="ACHAT0_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451438.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACHAT0_001493"
     CDS             complement(15797..16069)
                     /locus_tag="ACHAT0_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM8451439.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..>16275)
                     /locus_tag="ACHAT0_001494"
     CDS             complement(16066..>16275)
                     /locus_tag="ACHAT0_001494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451440.1"
                     /translation="GTVVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMN
                     DKYLGEGFCGDSLFDDERILKEETE"
BASE COUNT         4558 a         3446 c         2532 g         5739 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcc
//
LOCUS       DAYYAR010000052        16275 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000052 DAYYAR010000000
VERSION     DAYYAR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.052"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..674)
                     /locus_tag="ACHAT0_001495"
     CDS             complement(<1..674)
                     /locus_tag="ACHAT0_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFM8451441.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEK"
     gene            complement(894..2003)
                     /gene="rpoD"
                     /locus_tag="ACHAT0_001496"
     CDS             complement(894..2003)
                     /gene="rpoD"
                     /locus_tag="ACHAT0_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8451442.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(2027..3868)
                     /gene="dnaG"
                     /locus_tag="ACHAT0_001497"
     CDS             complement(2027..3868)
                     /gene="dnaG"
                     /locus_tag="ACHAT0_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8451443.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(4050..6365)
                     /locus_tag="ACHAT0_001498"
     CDS             complement(4050..6365)
                     /locus_tag="ACHAT0_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8451444.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(6550..7410)
                     /locus_tag="ACHAT0_001499"
     CDS             complement(6550..7410)
                     /locus_tag="ACHAT0_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8451445.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(7469..8101)
                     /locus_tag="ACHAT0_001500"
                     /pseudo
     CDS             complement(7469..8101)
                     /locus_tag="ACHAT0_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            8268..9029
                     /locus_tag="ACHAT0_001501"
     CDS             8268..9029
                     /locus_tag="ACHAT0_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8451446.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            9055..11646
                     /gene="mprF"
                     /locus_tag="ACHAT0_001502"
     CDS             9055..11646
                     /gene="mprF"
                     /locus_tag="ACHAT0_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8451447.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11834..13021)
                     /gene="tuf"
                     /locus_tag="ACHAT0_001503"
     CDS             complement(11834..13021)
                     /gene="tuf"
                     /locus_tag="ACHAT0_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8451448.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            13389..13745
                     /locus_tag="ACHAT0_001504"
     CDS             13389..13745
                     /locus_tag="ACHAT0_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8451449.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(13986..14990)
                     /locus_tag="ACHAT0_001505"
     CDS             complement(13986..14990)
                     /locus_tag="ACHAT0_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8451450.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            15156..16007
                     /locus_tag="ACHAT0_001506"
     CDS             15156..16007
                     /locus_tag="ACHAT0_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8451451.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            16024..16269
                     /locus_tag="ACHAT0_001507"
     CDS             16024..16269
                     /locus_tag="ACHAT0_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451452.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         4652 a         3373 c         2861 g         5389 t
ORIGIN      
        1 tgtttttcag taaatacttc agttttttct gtgatatccg tttttacgtc ttttattttt
       61 actgtttctt tacctgtggc gttttctcta tcagagtctt tttcagtttc ttgttccttt
      121 tctggctcta ctttgttttc ctgtgcaatt ttttcactga tcattttgag cagctctttc
      181 gcgtgctctt cttttgtatc ttcacgatgt gctttgatat aagcggatag tatcgaattt
      241 tcttcctcat tatcgacgat ttctttcagt tcagctactc ttcttttttg ttttgattca
      301 agcgtttctt cttgattctt tcctgattct tccgaagtct catgaacagt tgctttcttc
      361 tgttcatttt tctctatatt agttacttga tcgtgagcag gtggttcttc gtcttctggt
      421 tctgcttttt cgaataattc cattacagat tctagaggta aatctttcaa ttccgaccat
      481 tcgattggat cgtttaattc tgtatcagga aagactgcat cttcagttga tttatcctgc
      541 atagctgcat ctgtatttgt ttcttcaatt gattctttta tttgctgatc ttctgcagtt
      601 atttgatgac cgcagtctgg acagatagta ttctgctcca tttttttcga tccgcaattt
      661 ggacattttt tcatcactta gcgatcggct cctttactag gtttacgcta ccatttaatc
      721 tatcatattc tatacgtcac gccaatagct tatttttagg atttacttaa aaataatgaa
      781 tgaatttcct tcctatagaa aaaaaagctt gagactgcgt agtctcaagc tttttttctt
      841 ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca cgcttattct
      901 aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa tgctttagcc
      961 tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc ttccaatgtt
     1021 ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc ccgatcagtc
     1081 aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc gtgttctgct
     1141 ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc ttcttcaccg
     1201 attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg gactttttct
     1261 gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc aaggtcttgc
     1321 agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg aactggaata
     1381 cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat ccaccatgta
     1441 gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc cttcatcagt
     1501 cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac ataacgttta
     1561 gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc ttcttggtcg
     1621 ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag ggagacacgg
     1681 ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt tggcgcagaa
     1741 agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct gtgaattgat
     1801 ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg gatcaatttg
     1861 tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa atcatcatat
     1921 acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc ttcatacgtt
     1981 tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat ttggcttgtt
     2041 ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc tgtttgtttc
     2101 cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca gctaagccag
     2161 atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct tctgccaccg
     2221 gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa aagtctaaaa
     2281 actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa aaataaactt
     2341 ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta cggacagaaa
     2401 cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc gtcaaaggtc
     2461 tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc tgaggttctt
     2521 gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc gtctctctgc
     2581 cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt ggtgaaacaa
     2641 ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt tcattatcta
     2701 aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg cgcccatgag
     2761 ccactaattc ttggaaagat gcttctccat acttacggag atattcatca ggatcaagct
     2821 tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc aattcaatcc
     2881 ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc aattctttcg
     2941 tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata gaagctaatc
     3001 cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg aacaacaaaa
     3061 tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa acttgtcttt
     3121 tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt tcctgccctt
     3181 tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa ttacgaagtg
     3241 gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc gtgacaaaca
     3301 atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact tgctgaagaa
     3361 aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct aaagacaacc
     3421 cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat agcatgtgat
     3481 gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca gattcaggat
     3541 tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt tcgaagtctg
     3601 ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga aaagtaaaca
     3661 cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct gcaacagaaa
     3721 aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta ccagactttt
     3781 tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt actgtttcga
     3841 taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg atatcttttg
     3901 ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa ctgaaatcag
     3961 tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat tgctttttca
     4021 agtataacac atttctatct cattaagcat caatttgcta ctcttttcag taactgattt
     4081 tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat gaatacaaag
     4141 aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg gaacagcatt
     4201 gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa tgggttgaag
     4261 ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta atacatcaaa
     4321 gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac cgcaatgata
     4381 ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg caaataacgg
     4441 taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata cccttcaatc
     4501 gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc tgtaacggca
     4561 catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc tgagactttc
     4621 gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt agtaaaatca
     4681 gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc tgccatagca
     4741 atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc tgtcattgca
     4801 accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg cggactcact
     4861 cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc agtaatttga
     4921 gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat attggagaca
     4981 gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg aagataagct
     5041 agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc aaccggctca
     5101 acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa cataagtacg
     5161 cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc ttccaccaaa
     5221 cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc gccccatttg
     5281 cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag ctcaaaataa
     5341 tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata cgtccccata
     5401 atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg gatactcatt
     5461 ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg ggccagcgtt
     5521 tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt tacccctgtc
     5581 gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa caaagctgct
     5641 gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc ttccaaaaat
     5701 agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt gcgaatcgaa
     5761 tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa gcggacagca
     5821 taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt caaaaagctt
     5881 ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc tgtcaaaaga
     5941 gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat ctgttctcct
     6001 gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga ttgttcttta
     6061 ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg tacaagattt
     6121 cttgcatgta tttcttgata aattccaata acgatattga cgacaataat tgctaaatac
     6181 gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa gttcagaaaa
     6241 ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga tgcatcttcc
     6301 tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg tgtaagtcca
     6361 ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg tttaagtcta
     6421 gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa cacacgaatg
     6481 attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt ttttattatc
     6541 aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta gtgctgtacc
     6601 ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg cgtaatcggt
     6661 aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga atagcttgct
     6721 atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac aaagaatccc
     6781 accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt cagccatcgc
     6841 tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga cgatcatacc
     6901 caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga cttgcccgtc
     6961 aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa caaccatgat
     7021 gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg caggaacagt
     7081 tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac ctaataaaaa
     7141 actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa caggcagaaa
     7201 aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa tcagccgctc
     7261 atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga taaatcctga
     7321 accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt accccacttc
     7381 ttgattttcc attttttgcc ttttttccac tatttgcgta ctccttcttt catttaattt
     7441 tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag gtctctatta
     7501 ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta tgatagagat
     7561 agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc ccttcctcat
     7621 ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct actgtgtttt
     7681 ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat gcttggacag
     7741 ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc aaatatttga
     7801 caccttgatc aatactgtca gctggattat caatgatctc agcttctcca tgcagacttt
     7861 ccgagctctg catgagatct gtcccttttc cttttgattc ggtcaatatt atcgccttga
     7921 tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt tctatttgat
     7981 agctttccac tcgttttacc gaacgataag ttcttcctca taaaaacaac accgcaccaa
     8041 taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt tttcgcttca
     8101 tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta tcttcttggc
     8161 tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct gtattttttg
     8221 acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg actgatataa
     8281 tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat gcaaaaatgc
     8341 cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc tttttactgc
     8401 atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc gaacgctact
     8461 ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa gcatctatgt
     8521 tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa aaaatcgatc
     8581 aggcagattt tcttggattt agtgatggag caaatctagc actggtattt gccagcagct
     8641 tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt aaaggcgttc
     8701 gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta ctctctttat
     8761 ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat gacatcgggc
     8821 ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg ggaaaaaaag
     8881 atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa gcttcctttg
     8941 tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc ttcaatcgag
     9001 aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat ttatttgtta
     9061 aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat ctttttgatt
     9121 tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct atctatccag
     9181 caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat gctattgatt
     9241 ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg gatgcttgaa
     9301 caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac gatcaacaat
     9361 attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata tggacaaaag
     9421 aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt gatggctgga
     9481 ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt tgctcctttt
     9541 ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt cgtcttttta
     9601 tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac tcaaggacag
     9661 ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct ttctgttggt
     9721 tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat tgctgcttca
     9781 gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt aatgatgatt
     9841 atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat cttgctctat
     9901 cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt caaaaatatc
     9961 ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac agaaattgcg
    10021 cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact ttctgccact
    10081 atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt gaaatttcat
    10141 tttattatcc aatttcctag tatactattg ggatttcttt taatcattat gggcagaggg
    10201 atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt tctagcttta
    10261 ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc tattctgctt
    10321 ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta ttcatgggag
    10381 tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat cgttattggg
    10441 gtatacaatc tgccggattt ccctcatcgg cgtcaccatt ttatttcctt tttcttattt
    10501 ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag tttcatgatc
    10561 gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc ctttaatgaa
    10621 gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca attgatcttc
    10681 ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt tcttctccaa
    10741 ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa gaaagaagat
    10801 tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta tttaccagtt
    10861 ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta cgatttcatt
    10921 aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg gaaaaaaatg
    10981 aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt tgatgtctta
    11041 caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga taattggttg
    11101 ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta tctacaaaga
    11161 gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc aacgatcatg
    11221 cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga ttctaaaaaa
    11281 gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat gaaagaagaa
    11341 catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac ttccagaaag
    11401 agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca tttttattct
    11461 ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc acgttatact
    11521 ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat tattgataat
    11581 gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat acgaagaaag
    11641 tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga gactgaagta
    11701 aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa agagcggtcc
    11761 aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta ccaaaatatt
    11821 cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact gtccgtccgc
    11881 cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc aagtcaacat
    11941 caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat gtgattgttc
    12001 ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag aatggagtat
    12061 gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt gtatgtggag
    12121 taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt tggatccccc
    12181 gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta cggaacattt
    12241 ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc tctacctcat
    12301 caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct cgacctgtaa
    12361 ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt tctggtgttg
    12421 ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca tctggatcgc
    12481 cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct gggaaaccat
    12541 attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct tcatcatcga
    12601 ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg cgtgacaata
    12661 aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact aagattgcac
    12721 catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt cctggtgcat
    12781 caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta ttgatcgtaa
    12841 tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga ggatttgcta
    12901 ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc ccatggtcaa
    12961 cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt tcttttgcca
    13021 taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct caccttttta
    13081 ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt tgtaagattc
    13141 gaactaagct tattatacgt caatattggt caaagtcaac taagagcact cgtctgtctt
    13201 atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt tttttgagaa aaacttcaag
    13261 atagtcctta actttctttt gaagcaataa gaacaaaatg aggaaaagtt taaaattcag
    13321 ctgttgccaa caactttcaa tagacgaaat cttctatttc gttaataata aggaaagagg
    13381 tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt aggcatattt ctcggcggaa
    13441 gttttgcagc ttatcattat ttttacggcg gcgaagctta ttatacaaaa atcacgacga
    13501 atggcgaaat gtcagctacc caagcagata acggagagaa attcacgact tatacctaca
    13561 agcaagcggc ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc gaagaaagag
    13621 aacatccatt aaagatgaat gcgtatctta aactcaaagt caatcctcgt aaaggtgtat
    13681 taagctggga agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa aaaatcgata
    13741 attagtaaat ggtcttaaaa ttttattaga taaaaagagg ctgggacaga agcgtttaac
    13801 tccaagaaat aagaaggagt tgcttctgct tccgccgttt atacgtgttt agagcgtgag
    13861 acaaaagtgt tctttacttt tgtctcacgc tctttttttg ttatattcgt cttttccgat
    13921 ccttctcttg acacaattca tttaactgat cactggtact tgaacaccta atattctagt
    13981 catttttata aacgattcaa tacttcatcc acatttttga tcatcacgga tatcgtacca
    14041 tccggattat tcacaaattc aatcaaatct gggttgcgat atacatctac tggtacgatc
    14101 agttcgatcc cgtttgatag cttgaatttt tgttttccaa attttttctc agaaatttct
    14161 tgtacttctt ttacaggagg tgcttctggg acaaatcctt gctcttgtac ttcttcttta
    14221 aaagccattt ttgccgtgat gttttccttg aagacttttt ccgcaatcat ctcatgatcc
    14281 aaccgacctt tttcttctat cgtatcatag acagcttctt tcaaatcagc catgatatca
    14341 aattcttctg tctcaaattt ttttccaaga tgcttggcta ctttttggat cacttttaca
    14401 ttttcttcta aagaaggtgc aggaacactt tcgatcactc ttccagaaaa ataaagctct
    14461 tttttgccag aaaactcata ttttttttca attaattcgt aggtccaatc agtcaaattc
    14521 acagcgaatg cttcatctgc tttttgtgtt ttcgccccta gaattgctcg attcaaaatc
    14581 aagcggttgt cgatccctgc ttcgtctgct tcaacaaagt gagtgtaagc ctcacgataa
    14641 ttgactttca ataaagataa gtacatcact gtatctaatt catacaagac aacaaataca
    14701 tctgcactag gtgcttcttc actttcttgg tagatatcga accaccgctg aacgattgct
    14761 tcacttgctt ccacaaaatt ttcttgagcg attttaagtg attcaacgat catgctttct
    14821 tctgctagtt gtccagtttt tgtttgagca gaagagattt tttggatctt cttttgaaga
    14881 aaatcacgaa tatattcttt tgtcagatcc agctcttttt gtgaaaagac tggagaacca
    14941 gtttctctgt caataatatg cagaattgct ttttttaaat agatatccat tttctcattc
    15001 ccttctgtct tagtttacgg aaaaaaaatc aaaaatacca gaatcaaaat cattttcttt
    15061 taaacaaaaa tttcaagagt agaaacaaaa tggattcttt ttttcgaaaa atgcgctaca
    15121 cttatagttg aacgaataaa aaaggagctg aaagtatgaa ttcattaaca agcacttatc
    15181 gtctatcaaa tggttacgaa atcccggtag taggattcgg cacatggcaa acacctgatg
    15241 gggatgtcgc tgtttcctct gttaaagaag cgctggctgc aggttatcgc catatcgata
    15301 ctgcccaagg atacaagaat gaagaaagcg tcggccaagc gattaaggaa agtggaattc
    15361 ctagagaaga gatattcttg acaacaaaat tatggaatgc aaaccacagc tatgaacttg
    15421 tgatgtcttc ttttgaagaa tcattgaaga agctccaaac agattattta gatctgtttt
    15481 tgatccactg gccaaaccct gttgcttttc gtgataattg ggaacaagca aatgcagata
    15541 catggcgagc atttgaagaa ctgtatgaag caggtaaaat caaagcaatc ggtgtgagca
    15601 atttcttacc ccaccatcta gatacactag caaaaacagc aaaaattatg ccgatggtca
    15661 atcaagtttt ccttgcacca ggcgaattac agacaactgt agttgagtat gcaaaaaaac
    15721 atgatatgat cctcgaagca tatagtccat taggcactgg taaaatcttc gatgtgcctg
    15781 aaatgaaaca aatcgctgaa gcacatgaca agacgattgc acaagttgct ttgcgttggt
    15841 cgctgcaaca cgaattcctt cctcttccta agtcagtgac accaagcaga atcaaagaaa
    15901 atactgaatt atttgatttt gaattaacag aagaagaaat gaaacaaatc gatcagttag
    15961 atggtgtagt cggcaaagca aaagacccag atactaccca attttaagtc gaaaggtgat
    16021 tttatggatc caatcgaacg cttgaattct ctctcggaag aagttaccca gacttttcat
    16081 tccgattttg tctttttgat cgatgcagag aaaatccaac attttcctgc aagaaactgg
    16141 acacatgatc aaatcatcga agaactgaaa aaacggttcg atcattcgct aatggtcaaa
    16201 ccctggcacg aacatgaagt catttattca cctgaactac ctgtttttgc cttgataccg
    16261 aaaaaataga aagaa
//
LOCUS       DAYYAR010000053        16269 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000053 DAYYAR010000000
VERSION     DAYYAR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.053"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..788)
                     /locus_tag="ACHAT0_001508"
     CDS             complement(<1..788)
                     /locus_tag="ACHAT0_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8451453.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSS"
     gene            908..1819
                     /locus_tag="ACHAT0_001509"
     CDS             908..1819
                     /locus_tag="ACHAT0_001509"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8451454.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            1826..3472
                     /gene="mdcA"
                     /locus_tag="ACHAT0_001510"
     CDS             1826..3472
                     /gene="mdcA"
                     /locus_tag="ACHAT0_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFM8451455.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            3574..3873
                     /locus_tag="ACHAT0_001511"
     CDS             3574..3873
                     /locus_tag="ACHAT0_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8451456.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            3863..5539
                     /gene="mdcD"
                     /locus_tag="ACHAT0_001512"
     CDS             3863..5539
                     /gene="mdcD"
                     /locus_tag="ACHAT0_001512"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8451457.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            5543..6136
                     /locus_tag="ACHAT0_001513"
     CDS             5543..6136
                     /locus_tag="ACHAT0_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8451458.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            6160..7236
                     /locus_tag="ACHAT0_001514"
     CDS             6160..7236
                     /locus_tag="ACHAT0_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8451459.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            7261..8130
                     /gene="mdcB"
                     /locus_tag="ACHAT0_001515"
     CDS             7261..8130
                     /gene="mdcB"
                     /locus_tag="ACHAT0_001515"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8451460.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            8241..9005
                     /locus_tag="ACHAT0_001516"
     CDS             8241..9005
                     /locus_tag="ACHAT0_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8451461.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            9494..11587
                     /locus_tag="ACHAT0_001517"
     CDS             9494..11587
                     /locus_tag="ACHAT0_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8451462.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            11612..11914
                     /locus_tag="ACHAT0_001518"
     CDS             11612..11914
                     /locus_tag="ACHAT0_001518"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8451463.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            11941..13230
                     /locus_tag="ACHAT0_001519"
     CDS             11941..13230
                     /locus_tag="ACHAT0_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8451464.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            13249..13656
                     /locus_tag="ACHAT0_001520"
     CDS             13249..13656
                     /locus_tag="ACHAT0_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8451465.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            13679..14548
                     /locus_tag="ACHAT0_001521"
     CDS             13679..14548
                     /locus_tag="ACHAT0_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8451466.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            14550..15170
                     /locus_tag="ACHAT0_001522"
     CDS             14550..15170
                     /locus_tag="ACHAT0_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8451467.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            15620..16225
                     /locus_tag="ACHAT0_001523"
     CDS             15620..16225
                     /locus_tag="ACHAT0_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8451468.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT         5363 a         2843 c         3502 g         4561 t
ORIGIN      
        1 ctagaagata aataaattgt tcgtgtcaag ggcaaatctt tgatttcccg caagacgaaa
       61 ggatcatttt tatcgtaaga ccaagaaact tttgggaaaa agctaacagc taatccttgc
      121 ttgacaatag aacgtaatgt cgcaggatca tctgtcgtcg aactgatcgt caattcgata
      181 cttttcgctt caagtgctgc atccagtgta tgacggaagg cgttgctctt tgacagcatc
      241 aagatggggg tgcgttggat atcttctaat tgcagatgtt cttttttagc aagaggatgt
      301 ttcttaggaa tggcgacata aacaggttct ttcatgatag gaatattggt catgccaggc
      361 ttcatctccg aagtgatgta cagtacagga tcggtaatat catctggtaa atgatgttgc
      421 atgatcgtta attggatatc aggatgcttt tccataaaaa ttcggaccaa gttcggaata
      481 gaaacagagg ctacctctaa atacaatatg acggaagtaa cgatcttctc attgaccgaa
      541 gacaataatt tgattccttc ttctaatgtg tctaatgtct tgactgcata tggataaaat
      601 tcttctgcat aaacggtcgt ctcaatgttt ctgccgctat ggtaaaacaa agggacacct
      661 aattcgtttt ccaaatgttt gatcgcacgg ctcaaggcag gttgagaaat aaataattct
      721 ttggctgctt gggtaacatt ttttgtttcg aatacttttt taaaatactg cagatgaagc
      781 aatttcataa ttatccatcc catccatacc tttttgttat ggttttttct ttttttggca
      841 ttttttatta tagcagaaat tgactaagat agcagtggaa aaagtaagtg ctgaggaggc
      901 gaggctcatg agtacaggtt tcttatttcc tggacaaggc agtcagaagt tagggatgtt
      961 agcggatatc cctgaaatgt acatggaacg tttagaaaat gtgacggggt atcggttagt
     1021 tgataaagaa gaaaactatc aagatacggt ttttatccag ctagcattac tgacaaaagc
     1081 agtattttat ttagatgaaa tggaaaaaaa cggtattcag cctgatattg cagcaggaca
     1141 ttcgattggt gctttttgtg cagctgttgc ttgtcgatct ttgagatttg agaatgcagc
     1201 ggaactagtc tatcaccgcg cagtcttaat gaaagaagct tatccaagcg gttatgcaat
     1261 gggcgtagta gtaggtgtga cgagaagcga agctgaggaa attgtagcaa aaacatttga
     1321 tcctgaccat ccagtttatc tgtcaaatga aaattgtccg atgcaacata cgttatctgg
     1381 gagtatcgaa ggtttatccg ttacgttggc agaagccaaa cggcaccaag cgcaaacagc
     1441 gaagctgctg aaagtccctg tgccatctca ctgtctcttg atgaatgata cggtcaagaa
     1501 attcaagccg tatatgcagt ctgtccatct atctgatgct agatgtttgt atataaaaaa
     1561 tacggatgga agagccactc aggacgcaga agagattcgt aaagatttgt tggaaaacct
     1621 agctttccct gtgcagtgga accaaatgat ggacttggca aaagaattag gaatggatct
     1681 gacgattgaa tttccccccg gcaatacttt gacaaagctg attcatacaa aatttggtgt
     1741 agataaaggg atccgtacga tcaatctaga ccaacatggt attgatgacg ccacgtttct
     1801 atatcaaaaa tggagatgac taattatgga aaaaccaaac cgcaactggg cttctaaacg
     1861 cgaaaaaaaa gcgcagcgat tggaaaaaat cagcaacttg ctcaatggca agtatgtaga
     1921 aactgaaaaa atcgtagaag taatggaaaa actgattgct cccggcgaca aagttgtatt
     1981 agaaggcgac aatcaaaaac aagcaagttt tctttcagaa agtcttgaaa aagtagatgc
     2041 agaaaaagtg aatgatctgc atttgatcat gtctagtatc tcacgaccag aacatttgaa
     2101 tatttttgaa aaaggaatcg caagcaagat cgatttctct tatgctggtg cacaaagctt
     2161 gcgtgtcgcc caaatgattg aagacggcac aatgaaatta ggtgatatcc atacttattt
     2221 ggaactttat ggacgcttat tcatcgattt gattccaaat gttgttttag tagctgcaga
     2281 taaagcagat aaagacggca acttgtacac aggtttcaat acggaagaaa caccaacaat
     2341 catcgaagct gcagctttca aagatggtat cgtgatcgtt caagttaacg aagttgtcga
     2401 ttctttacca cgtgtagata tccctggtga ctgggtagac gttgtcgtga aagcagataa
     2461 accttatcaa ttagaagctt tattcacacg agatcctcaa aacatcacag aattgcaaat
     2521 tctaatggca atgatggcaa tcaaaggaat ctacgcagaa cacggcgtac aatctttgaa
     2581 tcacggtatt ggattcaata cagcagctat cgaattgtta ctgccaactt atggtgaatc
     2641 actagggtta aaagggaaaa tcgcgaagaa ctgggtattg aacccacatc caacgatgat
     2701 tccagccatc gaatcaggtt gggttgaaag catccatagt tttggtggcg aagttggtat
     2761 ggaaaaatat attgctgctc gcccagatgt cttttttgtc ggtaaagacg gcacgatgcg
     2821 cagtaaccga acattatcac aagctgcagg acaatatgcc atcgatatgt ttatcggttc
     2881 aaccttgcaa ttagattacg aaggaaactc ttctaccgta acaaaaggac gtttatctgg
     2941 ttttggcggt gcgcctaaca tgggacataa tcctggcggt cgtcgtcatt ctacaccagc
     3001 atggcaatca cttcgcgaca atgatgatcc attagggaaa ggccaaaaat tagttgttca
     3061 aatggttgaa acatatggtt caaataaaaa accagtcttt gtcgaagaat tagatgcact
     3121 agctgttcaa aaacaagcag gcttagccaa tgcaccagtg atgatttata gcgaagatac
     3181 tacacatatc gtgacagaag aaggaattgc ttatttgtac aaagcaaaaa caaaagaaga
     3241 acgacaagca gcaattgcag caattggcgg tgttacacct cttggaatga ctagcacgaa
     3301 agaatcacta gatagcttgc gtgcagccgg catcgtgaaa ttaccagaag atctaggcat
     3361 caaacgtagt gaagcgaaac gttcattatt agctgcacaa accatcgatg atttgatcga
     3421 atggtcagat ggcctgtatg aaccaccaat gaaattcaga acttggtcat aaaattggcg
     3481 agaaaaacct tttgtcgcca tttacccaga tggaagaaga aagacgaata agcagaacgt
     3541 gtatgctctg ttttacaaaa aggggagaga tccatggaac aattagctta taaatatcca
     3601 acgaaacagc cgatcaataa gaaaatccat gtaggagtgg tcggttcagg agatttggaa
     3661 atcttgatgt ttccaacaga aaaaacagaa tctactgtaa aaatttgtac aggaagcgat
     3721 ggcttcggcg aagtatggaa caatgtatta acgagatttt ttgatcgtta tccaatttca
     3781 gcagatatcg tgatcaatga cttcggtgct acaccaggag ttgtgtacct acgattgacg
     3841 caagcaatgg aggaactttc agatgaaaaa tagttttgta gaattaaacg ccagagaacg
     3901 agtgaacgct ctacttgaca atggacgtga attgattgat ccattcgatc aaatcatcgc
     3961 gccaagttta ccagcacaag gaatcgtccc tgaaagtgat gacgggatcg ttgtcagccg
     4021 tggcacgatc aatggcaaac aagcagtagt aatcgctatg gaaggaaaat tccaaggtgg
     4081 cggtatcggt gaagtttccg gtgcaaaaat cgttgctgca cttgaacatg tgttagaaga
     4141 aaataaaaaa ggaaatgaga tctttccagt cattgtacta gatacaggtg gtgttcgttt
     4201 gcaagaagca aactacggac tgttatctat ttcagaaatc ggaaatgtaa tcattgctct
     4261 aaaacaatat gtaccagtca tcggtttgat tccaggacgt gtaggatcat ttggcggtat
     4321 gtcgattact tcagcattaa tgagttattt aatcgcaaca aaaaaagcga gagtcggttt
     4381 gaatggacca gaagttattg aacaagaagc aggcgttcga gaatttgatt ctagtgacaa
     4441 agacttgatt tggaatacat taggatctcg tcaacgggta gaatcaaaaa tcattgatga
     4501 attagtgact gatagtgtag aagcaatcaa agctgctgtc atcgcagcaa tggacgaacg
     4561 aaaagacacg catcgcagtg aaagctcagc attttattta tcattacttg attctttaga
     4621 tctttcaaaa ccaatggcca ttgacgatta caatgaagca attgctaacc atcaagcaaa
     4681 atcaattgac ttaccagaag tagcagaagg aaatgaacca gctgcagcaa gtgttggtta
     4741 cgattggttt gcggcactaa ctggcatgaa aaatccaact tcttctatta gtactgtgta
     4801 ttatggtaaa tcaagtaaat tcatagaaga agccatcgtg acatcaatcg tacctgatcc
     4861 taaaaacccg atgtaccgtg tacgaaacgg tgaagttggt ttagttgaag gtttccgcat
     4921 gggacaaatt ttggatcacg tttacgaaga agataaagac aaaaaagtga aacggccaat
     4981 catcgttgtg atcgatgtac caagccaagc atacggctat aacgaagaat tgatcggtat
     5041 ccatgtggct ttggctaata gtgctgctgc ttatgcaaaa ctccgtcaag ctggacatcc
     5101 agttattgga ttgattgcag gaaatgctat ttcaggtgca tttttagcac atggattaca
     5161 atcaagccgc ttgattgctt taaacaataa agaaattacc gtccaagcga tgtctaaaga
     5221 aagtgctgca cgtgtgacaa aacgttcggt tgctgaaatc gaagcagctg ctgaaaaagt
     5281 tccagctatc gcttacgata tcaataatta tacaaaacta ggagcgatct atcatttcct
     5341 agaagatatc accgaccaag ctgcttcaga acaaaatgtc agcaaagtca tcgaagcaat
     5401 caatgaagcc attcaagatg tccgcaatac aaatgacacg atgctgacaa atcgatacac
     5461 aaacgaaaca gccaaaacat tcggacgtgt cgaaacaaat aaagtccgtg ccaaaatgga
     5521 cgaagaatgg gatgcataaa aaatgattca agcacatgat attgtccttt ttgataaaca
     5581 tgaaatagaa gggttcacct tgcctgaatg ggtcgttcta actgaaaaga tgttcggaac
     5641 agttcgtcgt gcgtcgacgg atcacgcaga aatactaccg attggtttac gtggacctgc
     5701 ccgcaatcag agatggtcaa cagaaatctc aaaacatgcc gtcaaacaag tcattcatcc
     5761 gtgggacatc atcgaacaag aatcgtatag acaagagaaa atcatggatt atcctgttta
     5821 tcagcaattt catgctgcta gaaacatatt atcaggatgt aaatggggaa tcggcggcag
     5881 cttaggattt gaattatcga ctgggattcc ggcagtaaaa gaaaccagtg attttgattt
     5941 gctgctgtac gcaaattcac cgattgaatt gccaattcag gtaattcaat cgcatcctgc
     6001 tttttttgag caattcgata cccaagtagt gactcggcaa ggcggttttt cactaaaaga
     6061 gtatttgaga gagccagaga aaaaaattct attgaaaaca gtcgaaggac ctaaattaac
     6121 caaagaactt tggtaataaa gaaaaagaga gagaaagaga tgtttgaaaa caaaaaaatc
     6181 aaaatcaacg taagtgactt ccagaaagta tcagctgtaa cggctgttat gatgcaaacg
     6241 atattgagct ttgctttggc aaacagccac agcgcaacaa atacagcttt tcttggtacc
     6301 ttttacgtca tcgttaaata cacagcacca atgtttattt ttgcaattgt ttataatatg
     6361 gtaaaaacaa gtcagcattt gtcttattgg gaatttttga aagaaaagtt ttttgaacta
     6421 gtcgttccgt atcttctatg gacgacggct tatctttggc tatttccagc agtccaacaa
     6481 caaactccgt atacggatac tgcaagcttt ttactgaaat atgtcacagg tgatggagcg
     6541 cctcatttat ggtataccgt tatgatgctg cagatccaat tgttcatgcc gttttttgtt
     6601 tggctcggtt acaaagtatt tgctaataaa aaatatgtat ggccagtatt gattggttca
     6661 acgattttat atgtcgcatg gtatctgttc tatcaaacac aagtattgac tgggccttat
     6721 catgacagct ggtacttatt agatcggttt gttgctagtt tcatgattta tggggtagcc
     6781 gcatttgttt atcacgaaaa agtctatcaa tatttggaca gagtacgtta tcttttctta
     6841 cctgttgcct tagttatcgc gttcttttct gtccgttcat tgctcgcaca tccaggagac
     6901 ttgagctttg cgaatgcacc ttacttgaat acgatccaaa gtttatacag tttagtcatc
     6961 atctttgctg tcttcatagg cgcttccaaa atgattgtaa acgattcgcc aaaattgcca
     7021 ttgttcaaat ggttgtctgt ttatgcttat cgtacgtatt tagccaatgt atttgttttc
     7081 caagtactgt tattattgtt taaagatagt tggctgcagt taccttcagg agtcatgatt
     7141 cttgtagctt atctaatgac ggcaagttgt gcatttgctt taagctggtt acttcatatc
     7201 atttgggtag ctatcaaaaa aggtttcagc aagtaatagt aggaagaagg agtgagtgtc
     7261 atgataaatg acgaacgaaa aaaagcagtg actcagcaac tcgctgcgtt ggtagaggaa
     7321 gcattgatcg cagaagtaac attaagtcca aaacctggat tagtggatgc actggatgcg
     7381 ggtgcacatt ccgatatgga ctatgcttta tttcttaagt ctgctaagac gctaactcct
     7441 ttttttgaag aaatggctca aatagcatgg catcatgcta ttgatcagga gttaagagaa
     7501 agaattgctg agattgggcg aaaagcggaa aaggcaatgc tgattgctac aaatggcatc
     7561 aatacccata aaggagcgat ttgggcaatg gggctattga tcagtgtcat tgcacaaaaa
     7621 atcagtatgg aacagccttt ttctttatcg aaaatacttg atgatgtcag caagcttgct
     7681 gcttttcctg atacaaaata tcgtcataaa aaaaccactc atggtcatag ggtgaagcaa
     7741 aaatatggcg taaatggtgc atatgaagaa gcgatattag ggtatccgca tatcaggctt
     7801 gcaattcaag cagctaagga gctagataat gattcatatg tacagaaaca aatgcatatg
     7861 ttgttagttt tgatgagttc gctagatgac acatgcgtat tgcatcgcag tgatcagaaa
     7921 acgttagtgg aaatgcaaaa acttgctcat gctgctagtc aaagtgcttt accaaatcgt
     7981 ccatttacag cactgttgca cttttgtcag gaaaaccaaa tctctcctgg aggaagcgca
     8041 gatctcttgt ctgcaagctt gttcatgctg aagatagagg aattatggac tgccgacaca
     8101 tacatttgtc cgatgttact caatgactaa ataagacaaa attgctaaaa aaacagttta
     8161 tgcatctggt tattgactct tccttttagc aaggctatca taaaaatagt tttttgtaat
     8221 aaaagataac gaggaagaca atgaaaaaaa tcaattattt agcaatcatt ttgttagtgg
     8281 gcctatgttt gtttgctctg ccgacccaag gattcgctgt cgcgggtggt catggtggag
     8341 gtacatctag cggtcgaagt tcgggcagag gttcatttgg tggtcgttca tatgggggca
     8401 gttattttgg cagtcgttct tacggcagag attcttatgg gtattatggt ccaggtacag
     8461 gtccttctag ttatgagcaa acgggatttc ttgcattagc tgttttgtca gtaggagggg
     8521 tttctgcttt ttctatcgtc aagaataaaa gacaaaagaa aaggctgaaa gcggagatat
     8581 tgtcccgcat gtcaggaacg gctaaagaaa agaaagcaca gtttctggaa atccaaaaga
     8641 tattcatgaa gatccaatcc gcttgggaag aagaaactcc tgaaaaagcc agaagctgtt
     8701 atacagacaa gctgtttgcc gaacatcaga aagttctgca aaaaaacaaa gcagacggtg
     8761 taagaaatca tactaaaaaa ataactattc aggaagtagg aaattatcga cagatccacg
     8821 aaaatagttt tgccatccga attgatttca gatgctgtga ttattcagtc gatcgaaaag
     8881 atgggcgatt gatctcagga agtaagacac aacgacagta tttttctcag atatggtatt
     8941 ttgatttttc tgagaaagaa aaaagatggc aagccgattt tattcagccg attatgctgg
     9001 attgacagga aatatttatg aacggagttg tgacaaaagc cttgtcgcag ctcctttttc
     9061 tgaataaacg gtgtgccaga gatagcctat cggcaataag gcacgatttt gtaaaatatg
     9121 ggaagcgatt tttcaaaatc gcttcttatc gctcaaggct gaacatctct gtcacaacct
     9181 ctccactaaa tggtgtttaa aagcttgcat ctgcgataat aagaacaacc aaacgaaaaa
     9241 taggaagcac tattttagtt tagttgttct tattacttga tgcaggacag ctttttcaca
     9301 acctcttttt tgtatagatg ggcaaaattt ttgtaataat aggcgtatgt ctttttcgtt
     9361 gaggagatga aaaagcatat ggcttatttt ttgtttgctt tgcttacttt ctttccactt
     9421 caagtggcag aaatatctac tttttattat gtaagcggaa ccatatactt gaaatcagaa
     9481 ggagatcatt tctatgaaaa caagaacaat tgaaatactt cacagaatca tgacagccca
     9541 atatccagta aaagcaaaac atctagcaag tgagttcaat gtcagtcagc ggacgatccg
     9601 tcaggaagtt cttgaagcga atatctggtt gagcagtaaa aagcttccgg agatccgtac
     9661 aattcgcaac aaaggttttt tgttgaagct ggaaaaggaa aagcaagctc gattagaaac
     9721 tgaaataaaa gatgtgaaag acggcttttt gaatcggcgc gaacgtattt ttgatctagt
     9781 tttagccgtt gcttacagcc gaatcccgat ccatctaaac aaaaaagaag aagaatttca
     9841 aatctctaaa agtacattag atgaagatat gagacggtta cgagcaacac tggtcagtta
     9901 tgggatagag atcgtcagtt atggaaagca aggactggtt tataaaggtt cagaacgatc
     9961 tatccgtacg atgatctatg attttatcaa tcagaatctg ggaatgaccg acttctttat
    10021 gaatcaagat gcacaattaa caccggctca aaaaatcttt ctccattatt ttcctttggt
    10081 ggaaatcaaa aaaatacagg agatctattc caataagttg ttgaaacaag aagatgatat
    10141 ttataaaaaa caaatcctgc tttttacatt gatctggatc cagcgaatca ggaaacatga
    10201 atttatctct gccattaatt ggaaaaatac agaaataaaa gacagtagct tttctgcatt
    10261 tattgaccac atgatcgagg aatttcaatt ggacaatgtc ccgcaagtag aaagaaatta
    10321 tcttcgtttc actattgaga cgttcaatgc tagagatatc tccaattcaa tcgagtgggt
    10381 acaagctcaa ttactaacga tccaattgat ccaatttgta gagaacgaga cacacgtacc
    10441 tttccatcta aaggaagaag ctttattcga aaatctctat aagcacatgg ctgcactgat
    10501 tgtaagaatc aagaatcata tccaagtcat gaatccattg aaagaaaaca tccgaacaaa
    10561 ttatttccca atctaccgcg cagtcactca ttttgttcct acgatcgaag aagtaattgg
    10621 tggcaagata ctggaggacg agatagcttt tcttgtcatc cacttttcga cgatcgccag
    10681 tgtaatcaag cgagacttga attatgttta taaatcagtc gttgtctgta accatgggat
    10741 ggctactgga aatctgttag cagagaatct gaaagaaaag tttcctcaga tcgagattac
    10801 tgcagtttta agttcaaaag aaatcgaatt agtagataaa ttggatgtca atctggtttt
    10861 cagcaccttt caattgaatt atcgaaataa gccgctttta gttgtcgatc cgattttaac
    10921 tgagacgaat atcttggtta tagaagactt tttgaaaaga aatgaagaac tacagcggtt
    10981 agagcctaac agtcatgaat cgacggagtt attcactcaa ataatagatg tcatgaaaga
    11041 aagtggtggc cgagtagatc gaatcatcta tggcaagtta gaaaaattat tcgaaacaaa
    11101 caatttagaa atcaacaaaa gggagatcca gcctatgtta aaagatattt tgacggacag
    11161 caatatcatg atcaaagaaa aagccactac ttgggaggaa tcgattgaac tagctgctag
    11221 accattggtt aaagaaaaca ttgtcgaaga gcgctatgtc gaagcgatga ttcgtgcagt
    11281 gaatgattac ggaccgtata tcgtgattgg caaacacatg gcactagccc atgcccgacc
    11341 agaagatggg gtcaataaat taggagtcag cgtagctacg attgagcaac caatcgattt
    11401 cggtaatcct gaaatggatc cagtaaagat tattttttgt ttggcagcag tagactccta
    11461 ctcgcatctg aacatcatga aagaactaat tgaattgatc aacaacgaag agaagttgga
    11521 tcgattgatt gaatgcacaa atatcgaaga tttcaaacaa ctgctttttt cagtagttga
    11581 gaaataataa atgatacggg ggagaacaaa gatgaacaaa ttgaacattt tattcgtatg
    11641 cggtgcaggt ttaggaagta gttttgcagc acagatgtct gctgaggatg ttttgaacaa
    11701 attaggagta gaagccaaac tggaccattg tgatatttct tccgcagttt ccatgaaacc
    11761 agatgtgatc atcacagcag aaaattttcg ttcgcagttc gaaaagttcg cagttgatcc
    11821 taaaacggcg atgatctatt tgaaaaatat tgtttcaaaa aatgaaattg aagaaaaaat
    11881 caccccagtt ctgcaagata agggagttat ctaaatacaa aaagaaaaga gggtaaaaac
    11941 atgggcgtta tcaattttat catcgaaaat attttgactc aggcatcgat caccattgca
    12001 ttgattgcta tgttgggtct actactgcag aagaaatcag caggacaagt catctctggc
    12061 acgttaaaga cactcctagg atttcaagtg ttgagtgccg gttcgagcat catcgttgga
    12121 agtttaactt atttcgggaa aatctttact gaaggatttc atatgcaagg gatcatccct
    12181 tctattgaat ctatcaatgg tcaagcaatg aatgatttgg gtttaggtag agatattgca
    12241 ctgacgttct tagccatttt tgtattcaat attattttgg ctcgtttcac aaaatggaag
    12301 tacatttttt taactggtca agcaatttta tggatggcaa cgatgacgac agtctttggc
    12361 tattttgccg gtctgcgagg gatcgtattg atcttggtcg gtggttttat cggagcttgt
    12421 ttcgccattg ctatgccggc agtagcgcaa ccgatcattc gtaaaatcac tggatcaaat
    12481 gatattgctt taggtcattt ttgtacgatc gggtacttat ttgaagccgg tgttgctaag
    12541 ctttttggtg agaaaggcga aaataaaaag tcgatcgaag atatcaagtt gccgacacat
    12601 ttcgaatttc tgcaagatac ttatttgtct gtcatggtcg tgatggtccc gctatatatc
    12661 atcactgttt tatttgcagg cgaaccattc gcttctgaat tatcaggaga tcaaaactat
    12721 atcatgtttg cctttttaca agccatccaa ttcgtagtgg gtgtgtatgt attgctagct
    12781 ggtgtacgtc tactgttagg tgaaatcgtg ccggcatttc gagggatagc tatgaaatta
    12841 gttcctgatg cgatccctgc cttagattgt ccagttttct ttccttatag cccaaatgct
    12901 gtcattttag gtttcattac gacaacgatt ggtacgatta ttgccatgtt tactttgccc
    12961 atgttcggct tagcgatgat tttacctggt atgctaacca atttctttgc cggagggaca
    13021 gcagggatat ttggcaacgc agttggcgga cgccgaggag cgatcattgg cggtattgct
    13081 catggtttct ttattacatt attgcccgct ttgctagtaa cgatctttaa ttcgatggga
    13141 ttcaacaatg ctacagcaac cgatgtagat acagtagccg cagcactctt atatgcctgg
    13201 atcttaagcc cagttttgaa agcattttaa ttgaaagtag ggaaaatgat gtttggattt
    13261 tttaagaaaa aagaaaaaga accgaaaaaa gttctggcaa aagaagaaaa agccctatcg
    13321 ccagaagcga ccgaaaagat tcaatcagag attacacaat tgaaacaaga gatttccact
    13381 acccaagata agcataaatt agcgaaactt tatgaacaag ttggacttaa attttctgaa
    13441 ttgtatgtga acgatcaagc catccaatat ttagaaaaga gtttggaaaa taaacagacg
    13501 atcggagatg ggtataaaaa actgatgagc ctctataacc agaagcgtgc agatgctgct
    13561 cgtgctggtg atgatcaagg gatcgattac tacatgggca aaatggatga gatgcgacaa
    13621 attgcaaaac aagtaacgat caaaggaaac aaataaaaag ataaggtggc aataaaagat
    13681 gtatacaaca ttgaaagaag taacaaaaac agcagaagag ttgaatttta cagttggagc
    13741 ttttaatacg cataacctag aaatgcttcc tgctatgcta ggcgcagcaa aagaaatggg
    13801 ttcgccgatc attattcaaa caagtgtcga taccgctaaa tatatcggca tgcatgtgtt
    13861 agtcaatgta atgacagcta ttgcagaaga agaaatggtt gatgcagtct tgcacttaga
    13921 ccacgcccga gactttgatg atatcaaaga agcaatcgac agcggatata cttctgtcat
    13981 gtatgatggg tcccatctgc ctttcaaaga aaatatcctg aagacaaaag cagtagtaga
    14041 atatgcccat gcgcacggtg tttccgtaga aggagaactt ggaacaatcg gcggtacaga
    14101 agaaggaatc catgtcgctg aaaatgacaa ggtatataca agaccggaag acgcagtgaa
    14161 atttgtagaa gctacaggcg tggatgcatt agctattgct atcggtacga atcatggaca
    14221 attcaaatca aaaacagaag taaacattcc cttgctgaaa gaaatcgatg cagtagttga
    14281 tgtcccccta gttatccatg gaggaacagg tgtgaaagaa gaagattatc ctgaattgat
    14341 caataacggg atccgcaaat tcaatgttgg aacggagctt ttagtcaact ggacgaaagt
    14401 agctaaagaa acctttagtg aaacagaaat caacaagtct cttcgccata atgtgattcc
    14461 agcaaatgaa gcagtaaaag aaattgtcaa gcataagatt ggactgttta tgaacttgga
    14521 aagtccaatt aacttaggaa ataaatgaga tgaaaaagta tttcattctt ctggctggta
    14581 gtcctggtac aggaaaaaca tatcttatga ataaactacg ggaacgattt ccagatatgt
    14641 atgccatcac gctcgatgaa atcaaggaat actatgcaga atccatcggg tttaacaatt
    14701 tggaagaacg ggcggaacaa gaaaggacga aagtttatcc tttctattac aaagcacttg
    14761 atttgtatat ggaagcagga aagaaagtga ttgtgtccga gtatcctttt agtgataagc
    14821 aaaaagcacg actccaagaa ttagcagata gatacggata tcaggtgatc acgattcgcc
    14881 taacagcaga ctttgaagtg ttatggcaac gtcggtacaa acgtgatcgt gaatcagatc
    14941 gtcatttgag ctatatcatg gatcattatc actatggtga cgtattggaa gatcgaacga
    15001 aagggaccaa tcatattacg aaagaagcat tcaaaaagat catcgccgac cgaaaatatg
    15061 acaaattcca gttaggaaca ctatatgaat ttgatgtaac agactatact caagtggatt
    15121 acactccact attggataaa ttagaaaaag aaatcaatga caataagtaa caaagaaatg
    15181 gcttaatatc gaaaaattgg cttttgaacg ctgtttaccg agaggtcgtg agcctgtcgt
    15241 tcagctccga gtcacaagga gcaatttcaa aaaaacagct tctcatgttt gatgaatatt
    15301 gtgacttgtg accgaggagt tggtaggaga acaccgttta gtggggaggt tgtgaaaaag
    15361 ctgtcctgcc tcaattaata agaacagcca gacaagaata gctcgtcata tttttcgttt
    15421 ggtggggctt attaccgcag atgcaagctt ttgaacgcca tttattcaca gccccagaga
    15481 agtcgaatgt cttttgttct cgagtttttt aacattcttt tatttattgc agatttcatt
    15541 atatttattt gccgtaatta aaccagtctt ctatacttga ttacatagtc atacatttgc
    15601 taaaaaagaa gggaagagaa tgaacaagaa gttggctcaa tacaaaagac tactcgcact
    15661 ttctcaagct ggtctatatt atggaaaaga tgtatttgat aaagagcgtt ttgaggagat
    15721 ccggacgatt tcattggaac tgatcagtga aataggaaaa gaaactttcg aagaaataaa
    15781 agcgctcaca acgattggag aaggacatcc aacaccaaaa gtagatgttc gtgcctatat
    15841 caaaaaagat ggcaaggtcc tactaatcga agataaacgg acgaaagaat ggtcacttcc
    15901 tggtggattt gctgaaatag gattgtctcc tgaagaaaat gtgcgtaaag aagtctatga
    15961 agaaacagga ttaacagtag aaactactca gttgcgagca gtttttgata cgaataaaca
    16021 aaaagatatt ccacagcttt ttcagtatta taaacttgtt tttgcttgtg cgatccacgg
    16081 tgaagaggcc aagtttattg agaataatga aacttccaac atgggatttt ttagtataga
    16141 agagttgcca aagctatcag aaaagcgaac aacgaagaaa cagttactta ttttagaaaa
    16201 caaaaaccag atctattgtg attgagcttt ttcgtaaaag aagaaaagaa tatactttac
    16261 tagttatta
//
LOCUS       DAYYAR010000054        16227 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000054 DAYYAR010000000
VERSION     DAYYAR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.054"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACHAT0_001524"
     CDS             complement(24..722)
                     /locus_tag="ACHAT0_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8451469.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACHAT0_001525"
     CDS             complement(715..2067)
                     /locus_tag="ACHAT0_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8451470.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACHAT0_001526"
     CDS             complement(2290..3690)
                     /locus_tag="ACHAT0_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8451471.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACHAT0_001527"
     CDS             3851..4588
                     /locus_tag="ACHAT0_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8451472.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACHAT0_001528"
     CDS             4713..5192
                     /locus_tag="ACHAT0_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8451473.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACHAT0_001529"
     CDS             5189..5956
                     /locus_tag="ACHAT0_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8451474.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACHAT0_001530"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACHAT0_001530"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8451475.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACHAT0_001531"
     CDS             complement(6859..7461)
                     /locus_tag="ACHAT0_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8451476.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACHAT0_001532"
     CDS             7567..7797
                     /locus_tag="ACHAT0_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451477.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACHAT0_001533"
     CDS             7788..8036
                     /locus_tag="ACHAT0_001533"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8451478.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACHAT0_001534"
     CDS             8142..8726
                     /locus_tag="ACHAT0_001534"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8451479.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACHAT0_001535"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACHAT0_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8451480.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACHAT0_001536"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACHAT0_001536"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8451481.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACHAT0_001537"
     CDS             10627..10806
                     /locus_tag="ACHAT0_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451482.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACHAT0_001538"
     CDS             10828..11853
                     /locus_tag="ACHAT0_001538"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8451483.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12044..13288
                     /gene="glyA"
                     /locus_tag="ACHAT0_001539"
     CDS             12044..13288
                     /gene="glyA"
                     /locus_tag="ACHAT0_001539"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8451484.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13441..14388
                     /locus_tag="ACHAT0_001540"
     CDS             13441..14388
                     /locus_tag="ACHAT0_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8451485.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14522..15151
                     /gene="upp"
                     /locus_tag="ACHAT0_001541"
     CDS             14522..15151
                     /gene="upp"
                     /locus_tag="ACHAT0_001541"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8451486.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15290..15532)
                     /locus_tag="ACHAT0_001542"
     CDS             complement(15290..15532)
                     /locus_tag="ACHAT0_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451487.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15653..15725
                     /locus_tag="ACHAT0_001543"
     tRNA            15653..15725
                     /locus_tag="ACHAT0_001543"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15686..15688,aa:Thr,seq:cgt)
     gene            complement(15844..>16227)
                     /locus_tag="ACHAT0_001544"
     CDS             complement(15844..>16227)
                     /locus_tag="ACHAT0_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8451488.1"
                     /translation="SVFLQQILKWGLTGNPINEAFSPLLFIILPIMILGEELFSVYFL
                     AIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGVARLLFNQAAIKS
                     NSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5533 a         2899 c         3277 g         4518 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaattgtagg aaaataaaaa ggagctgttg tttgtggtag attacaaaac
    12061 gtttgaccct gatctttggg ctgcgatcgc caaagaagaa gaaagacaag aacataattt
    12121 ggagctgatt gcctctgaaa actttgtctc cgaagcagtg atggctgcac aaggaagtat
    12181 cttgacaaat aaatacgcag aaggttatcc agggcatcgc tattatggtg ggtgtgaatt
    12241 tgtcgatatc gttgaaaatt tagcgattga ccgtgcaaaa gaattattcg gagcgaaatt
    12301 tgcgaacgtc cagcctcact ccggctctca agccaacacg gctgcttatc tcgctttggt
    12361 agaaccaggg gacacgatct taggaatgga tctttccgca ggcggacatc taacacatgg
    12421 atcaccagtc aacttcagtg gcaaaaccta tcactttgtt gcttacggtg ttgatccgac
    12481 aacagaagtt attgattata atgtggtacg tattttagct agaaaacatc aaccaaaatt
    12541 gatcgtagca ggtgccagtg cttacggacg aacgatcgac tttgcgaaat tcagagaaat
    12601 cgcagatgaa gtaggcgcaa aactgatggt agatatggca catatcgcag gattagtagc
    12661 agcaggactt catcctaatc cagttcctta tgcagatatc acaacgacta ccactcataa
    12721 aacattgcgt ggccctcgag gcggcatgat tttgacaaac gatgaagcat tagcaaaaaa
    12781 aataaatagt gcggtatttc ctggtattca aggcggccct ctagagcatg tgattgcggg
    12841 taaagcagta gcattcaaag aagcattaga tcctgcattt aaagaatata gtgaacaaat
    12901 catcgccaat gcaaaagcaa tggtcaaagt tttcaatcaa gcaattggaa ccagagtcat
    12961 ttcaggggcg acagataatc atctgatgtt gatcgatgtt cgcgagttag gtataaatgg
    13021 aaaagaagca gagagcattt tggacagtgt gaatatcact gtaaataaaa actcgattcc
    13081 ttttgaaaca ctaagtccat tcaaaacaag cggtattcga atcggcacgc cagcaatcac
    13141 tactcgcggg ttcaaagaag aagatgctgt aaaagtggct gaactagttg tcaaagcatt
    13201 acaagcaaaa gatgataacg ctcaactaga cgaagtcaaa acaggtgtac gcgaattaac
    13261 tgaaaaattt ccgttgcata aaaaataaaa atttaaattc cattagtaaa cagaaaagtt
    13321 caatgttggg cttttctgtt tattttttgg gaaaacggat tctttgacaa tatcggtgat
    13381 gctctctagt atatgaggtg taattttagt cttcacacta aatctcaggg aggaaaagtc
    13441 atgactgatc gtatctcgtt agaaaaggaa gcagtagcat tcagtgatgc aaatgcgcct
    13501 catccgcgta tctatgaact agcaccggaa gaaggaagag ttttactaga agaagtacaa
    13561 gaatcacctg tgaacaaata ttccgtcgat attgaagact tgatggtgga tactggcgaa
    13621 tggggaaaac tcaatattcg tctcgtacga ccagaaggga atatggataa attaccagtt
    13681 attttttata ttcatggagc aggctgggtt tttggcagtg ctcaaaccca tgataaattg
    13741 atcagagaac ttgcggttcg tacgaattcc gtcgttgtct ttccagaata tagtcgatca
    13801 ccagaagcaa aatatccaac agcaattgaa caaaattatg ctgtgttgca acagctgaat
    13861 aaattagcca agtcaaaaaa tctggatctc gagaatctaa tcgttgcagg agattcagta
    13921 ggtggtaaca tggctacagt catgacgatc ttaacaaaag aacgaaaggg cctcccaata
    13981 tccaagcaat tgctgtatta tccagtcaca aacgctgatt ttgatacaga ttcctatcat
    14041 gaattcggag aaaactattt tttgacaaga gaaggaatga tgtggttttg ggaccaatat
    14101 acgacgaatg aagaagagcg tgctgaaatc acagcttcgc ctttgagagc aaatataaat
    14161 gaactaaatg atcttccttc agcaatgatt ttaacgggag aagcggatgt attaagagat
    14221 gaaggtgagg cttatgcgcg caaacttcgt gatgcagggg ttcctgttac acaagtacgc
    14281 ttccaaggaa tcatccatga ttttgtcatg gtaaatgcta tggatcagac aaaggcaacg
    14341 cgtgcagcaa tggcattatc tacttcttgg atcaagaaaa aagaataagc tttgcttgtc
    14401 cgcaggaaaa cttcttttca aaaacggatt ttcttaggaa aaatggcgag tttgactagt
    14461 caatcacttg attttgtttt acaattaaaa agaaacgaca gaacaaagga gagcttaaaa
    14521 aatgggcaaa tttcaagtga tcgatcatcc attgattcaa cacaaactaa ctattattcg
    14581 ggacaaaaac tgcggaacaa aggtttttcg tgaagtagtc gatgaaatcg ctatgttgat
    14641 ggcatatgaa gtatcacggg atatgccatt ggaagacatc gtgatcgaaa caccgatcac
    14701 tgaaacaaca caaaaaacgt tgtctggtaa aaaagtagcg attattccaa tcttgcgcgc
    14761 aggtattgga atggttgacg gtatcttgga attgattcca gctgctaaag tgggacatat
    14821 cggtctttat cgagatgaag aaacattgga accacatgaa tatttcgtca aacttccaga
    14881 agacattgat gcacgtcagt tgtttgtagt agatccaatg ttggcaacag gcggatcagc
    14941 aatcatggca atcgatgcat tgaaagaacg tggcgcgtcg aacattaaat ttgtttgttt
    15001 ggtagcagca cctgagggag taaaagcttt gcaagaagcg catcctgata ttgacatcta
    15061 tacagcttct ctagatgaaa aattgaatga acatggttat atcgttcctg gactagggga
    15121 tgcgggtgac cgtttgtttg gtacaaaata agtaaatacg aaaaagggtg tgacacaact
    15181 tttgtcacat ccttttttgg tcatttttaa cgtttgatgc agaacagctt tttcacagct
    15241 ttcgtttgca tataaaaaaa ccatcccaaa aatgcagaga tggtcaaatt caaatctttt
    15301 ttagttcttt gtagttaacg attactacac ccttactttg cgtcatcaag ttcgcatttt
    15361 ctgcagtttc atcaatctca acaacagcgg aatttttcaa ttgctttgaa atcgttccag
    15421 tttgcgactt gccgtgaatt gtgaacatta cgtgagtatt gatcttgaat ttttcaccaa
    15481 tttccgatgg tacgatagtt gtatcaaatt ttccaaaagt tgccattttc ataaaacctg
    15541 cctttcacat caattatacc acgttttatg aaaaatatca tgaaattaaa ctaattttcc
    15601 atcggactct ttacaatcag ccaaatagag tatataattt cttttggata aggccagctt
    15661 agctcagttg gtagagcaac gcactcgtaa cgcgtaggtc gtaggttcga tccctatagc
    15721 tggcattaaa agagaagaca gatatatcaa ggatttttaa tccattgata catctgtctt
    15781 tttgtttaaa ggaaataaag tatgctttct tagcaataga gcatgtatta tttctataaa
    15841 aatttaggag aataaaacga ctagcaatat agctgtgaaa tcaaatatca catgaactgc
    15901 ccaacttgta atgatcgagt tgcttttaat ggcagcttgg ttgaataaca atcttgccac
    15961 cccttgtatc aaaaggatgt gtgctacagt gtgtaaaata tttccattat cataagtact
    16021 gtaatggata aatccaaaga tgattgctga caagaaacta gcgacaggaa tgctgaactt
    16081 actagaaaaa atagcgagaa aatacactga gaacaattct tctccaagta tcatgatagg
    16141 caaaataata aataataacg ggctgaaagc ctcatttatt ggattgcctg ttaaacccca
    16201 tttcaaaatt tgttgcaaaa ataccga
//
LOCUS       DAYYAR010000055        16211 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000055 DAYYAR010000000
VERSION     DAYYAR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.055"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..468
                     /gene="scm"
                     /locus_tag="ACHAT0_001545"
     CDS             <1..468
                     /gene="scm"
                     /locus_tag="ACHAT0_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8451489.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTS
                     DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(623..1609)
                     /locus_tag="ACHAT0_001546"
     CDS             complement(623..1609)
                     /locus_tag="ACHAT0_001546"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8451490.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1632..2198)
                     /locus_tag="ACHAT0_001547"
     CDS             complement(1632..2198)
                     /locus_tag="ACHAT0_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8451491.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2212..2628)
                     /locus_tag="ACHAT0_001548"
     CDS             complement(2212..2628)
                     /locus_tag="ACHAT0_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8451492.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3085..5019)
                     /locus_tag="ACHAT0_001549"
     CDS             complement(3085..5019)
                     /locus_tag="ACHAT0_001549"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8451493.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5321..6256
                     /gene="pplA"
                     /locus_tag="ACHAT0_001550"
     CDS             5321..6256
                     /gene="pplA"
                     /locus_tag="ACHAT0_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8451494.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6346..7449
                     /locus_tag="ACHAT0_001551"
     CDS             6346..7449
                     /locus_tag="ACHAT0_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8451495.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7462..8415
                     /gene="menA"
                     /locus_tag="ACHAT0_001552"
     CDS             7462..8415
                     /gene="menA"
                     /locus_tag="ACHAT0_001552"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8451496.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9229..10575)
                     /gene="gor"
                     /locus_tag="ACHAT0_001553"
     CDS             complement(9229..10575)
                     /gene="gor"
                     /locus_tag="ACHAT0_001553"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8451497.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10709..11962)
                     /locus_tag="ACHAT0_001554"
     CDS             complement(10709..11962)
                     /locus_tag="ACHAT0_001554"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8451498.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11968..12891)
                     /locus_tag="ACHAT0_001555"
     CDS             complement(11968..12891)
                     /locus_tag="ACHAT0_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8451499.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13112..15604)
                     /locus_tag="ACHAT0_001556"
     CDS             complement(13112..15604)
                     /locus_tag="ACHAT0_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8451500.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15611..16078)
                     /locus_tag="ACHAT0_001557"
     CDS             complement(15611..16078)
                     /locus_tag="ACHAT0_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8451501.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4763 a         3390 c         2881 g         5177 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgatagctc agattcatct
      121 accactacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga tacatcctct
      181 accacaagtg atagctcaga ttcatcaact tcaagcgata gtacagacac gtcgtctacc
      241 acaagcgata cacgtcatgc aagtgtctcc ttctcaaaaa caagttcgga taacaggacg
      301 aactttaact taactaataa aacaagcaat gacaatggaa acaaatcaaa aagagcactt
      361 cctaaaactg gaagtcaatc gaataattgg ataacactcg caggcgttat attacttgta
      421 atcggtttaa gaatcacttt tatgagttat aagaaatcga aaaggtaaaa aaatattcaa
      481 gataaaaaag agcaacgtcg ctaacggacg ttgctcttta tttttgcttt ttatcataaa
      541 tacaagaact acaattcaat ttgtcaaaaa acacgtagat ttccttctga gaaatttatt
      601 tacgtatcga acctttccct attcagtctt tgcgatccaa aatcagttgg gtaagggaaa
      661 agagattctc ttttgttcga agagaggtgg caggtaattt ctttatttcc ttcaaagcct
      721 tttctgtgta agaggccgct aacttcctag cttcttccac gcctcctgta tgttcaacga
      781 ttttgcgtac ggcgtcgaca tcttcttctg tcattgccgc tttcttctct aaataaggta
      841 gtagctttgg tttgtttttt tgcaaagagt agatcaacgg taaagaatag accccttgac
      901 gcatgtcttc tagtacaggt ttgccgattt cctggctctc ttttgtataa tctaaaatat
      961 catcgataat ctgaaaggcc atcccgatag aaagtccgat atctcctgct ttttttgcga
     1021 agcgctcgct tgttccactt tcaaaagcgc caacagaaca acttaattgg aacagttcag
     1081 ctgtcttacc tgaaatattt tccaaatatt cttttacaga aagttcataa ttatagcgat
     1141 catccatctg ccctaattcg ccattcaata ctttttccat actgcgagaa ttcaattgga
     1201 gacttttcaa ggaagtggag taatcagaca atagtttaaa acagcagaca aataaatagt
     1261 ctcctgcata gacagaaacg acattaccaa actgactacg gatattaggc tgaccacgtc
     1321 gcagatccgc ttcatcaacg atatcatcat ggatcagtgt ggccgtatgg agcatttcga
     1381 tagaagctgc caaagcgatg gctttgtctc gatcttgctc tggtccaaat tgtgagaaca
     1441 atagctgata ggcaggacgc aacagctttc cgccggcatg gatcgtttct ttgattgctt
     1501 gttcgacagg tttgtttttc aggcggacag tttcttccat ccgttttagt gtttctgtca
     1561 attctttact taaagcagga tagttttccc acattggatg tagcttcata aaaaaactcc
     1621 ttgtgtgacg attatttttt ggtaaacatt gggctccatt tttgtttggt tccctgctca
     1681 taatcttttt ggaatcgttg aagtgtctgc acatgtcgta aaagataatt cgctgcgatc
     1741 ccgattgcaa tccctgataa aatccctaaa actgaaagga ctggcaagta caacatgact
     1801 gtccatgatt gtgcaatgaa agaagccatc aaaagctgtc cgacattatg catgaaaccg
     1861 ccggccgcac taattccgat gatactgaca cgttttggtc ctaattgctt gaccaataac
     1921 attccaaaat aactaagcaa agcaccactc atactataaa ggaatgtgga aatcgttccg
     1981 cctagcaaag tagtcaggaa tagtcgtagc caaagtagta agaagctgtc ttgttttttc
     2041 atggtaaaaa tggcaacgat cgtgattaga ttggctaagc ctagttttgc accaggcgca
     2101 aaagaaaaag gataagggat catattttcc aataaaccaa tgatcactcc ttgtgcaaca
     2161 agtagcgaaa tataaatcat tttttgtaat ttactcatgg tgtgtaaagg tctaataaat
     2221 cagactgtct tcgtcacctc catctgttga acggacttcg atcactaatt tatgtggcaa
     2281 acagacgatg gtttcgccat ttttcgttgc ccagcctcga cgtacgcaga tctggtcatc
     2341 gcaatctgct tcttttatcc gtactcgatc gccatctatc tcgatcaagt tataatctcc
     2401 gtgttcatct ttgtaagtat atgtgttgct ctcttctcca gccatcaaag ggaatgtttt
     2461 gatttctgtg ccatccacac gcagtaccgc ttctttttct gctgttgcgg tgttttgctg
     2521 aagaccaaaa atcatgactg gtaaaaaaga aagaacagtc aaaagcaaaa taataaaaac
     2581 atcccaaggt ttgagcagac ttttttcagc aaattcttta aggctcattt ttgttctcct
     2641 ttttccagat taactcaccc aattttagca taaaaacaag aggttgtgac acctgtgtct
     2701 tgctttgagt aataagaacg aagaatagaa aacagctttc catgttttct aattctttgg
     2761 gcttattacc gagaagcagc aggtggaaca ccgtttataa caagaggttg tgacagaagt
     2821 gggcaacttc aagaaataag aaggaattta cgaaaattac ttttcaagtt tttgtgaaat
     2881 ttcggcttat ttttcgaaga agctacttct ggaacaccgt ttataacaag aggttgtaac
     2941 agaggttgtg acacctgtgt cttgctcatg gaggcttatc tcgggcatct tattccttta
     3001 attttacata aaaaggaggc tgtgacggtt tgtctcagcc cctttctatt gaaatcttgc
     3061 attcaaataa cgttctttat ctatttattt cttgccgtac tgggaacttg gtctaccgta
     3121 ccaccaatta cctagtgtac gttgtagcca tggaattacc cagcgatcca acccaatcgc
     3181 acgtccggaa ccgttcatca atgcgaaagc aacaaaaatg aaccaaatgt ttacccagta
     3241 aaacatgcct gataagcaga acgtgatcac caatacaatc gttgtcgcac tgcatagcca
     3301 tgtgaataat ccagcaatca atgctaatgc caaggcaatt tcaaagaata ccatgaattt
     3361 ttggaagaac attgctacat cattgtttgg catgatcact tgcatgatct ttgtgaacca
     3421 gtctggtgac ttctcaatca cagccattgg ttcttcaccg tatgcataac tcaaaccaaa
     3481 atgtgcagct tgagtcgctg cttctccggc acttgcccct gctccgctgg cagctgtggt
     3541 tgcatcacca gcagcagaag caccactggc aacatctgtt acattgtatt cccatgtcca
     3601 agggaacaca ctgtttccag tgaaccatga tccttcacca aaccaagtac ttggtttgaa
     3661 cacttcacca ttcccaatga tttttttgga agcttcaacg aaccaaacta aaccatagaa
     3721 aatacgaagt ggcacactcc ataagacatt gccgtaacga gaagaatgtc ctcgagtcac
     3781 gttgcgatca tctttgatat ggaaaaactc atgcattaaa tattggaaca tataataacc
     3841 agaacgaata tcaaagaagt atttcaaatt cacgatatgt ttcattacga tagctaaaaa
     3901 tccgcttaaa tggatcttat caaataggtt agctaccccc catttggcac caatagaaac
     3961 cataaatcct tgatagtttc ctttaaattt atgcttttct ccgcctttga tcgaagccac
     4021 gatattcgct gcggctgtgt gtcctgtttg ttcagcagct tgaacgatct gcggtgtcgg
     4081 tgttcctggg aattcttcat aaaagactaa atcaccaatg atatagatgt ttttatcttc
     4141 gtagcctttt gcctgcatat attcattggc gatcaaccgc tgtccgcgtg ctgattctaa
     4201 tccaaagtca gcagcgtcag aagttccttt gacaccagct gtccaaatca gtgtgtgagt
     4261 cggaatgttg cgtccgtctt tcaatttgat atgatcagca gccacttcta cgattggtgc
     4321 attcaataat aattccacgt tgtgcttacg caaatagcgt tcagcttttg cagcatcatt
     4381 tcgatccaac atattcaaga tcgtcggcat tgcctctacg accatcagtg tgatttcgtt
     4441 tggatctaat ttataatctt tggctaggcg atttttccag tcgatcagtt ccccaatcat
     4501 ttcaataccg gtaaatccag aaccacagac aacgaaagtc agcatggctt tacgcagttc
     4561 atcatctggt tccaatgcag cttttgctac tgtcttctca atatgttccc gaatccttac
     4621 agaatcttca aatgaccaaa gggtgaatcc gtgtgctttg acaccaggtg taccaaagtc
     4681 attcggctct ccgcccattc caaggatcag ctgatcaaaa ggataggaac ctaatttggt
     4741 cttgacgatt tttcgttctt tatcgatacc gattacagtg tctgtgacaa gttgtacatt
     4801 cttttgacga gcaaacaaat gctgcagatc gtattggatt gcttctggtt ctacacgtcc
     4861 gccagctact tcatgcaact cggtcatcat agtatggtac gaatgacgat cgatcagagt
     4921 aattgctaca tcttcttttt tcagtttctt tgccaagtat tttgtagctg aaacaccggc
     4981 atatcctgct cctacaacga caatattttg ctttgccatt gatcatccgc tccttataga
     5041 ctatctatat aatgaatata tgataattct tttagtttga tacttattac acaatctcca
     5101 tatattatat agatgttctg ttcttttgtc taactccttt tggatttttt tcaaatttta
     5161 aaataaagcg ttttttcacg aaatttgctg aaagaaaacg tttgaaatat tacatgggtt
     5221 atttatcaaa aatagttgac ttataggcta aaaagattag aatgtataat gaatcacaaa
     5281 gaaaaattgt ggactagaaa tggaatggtg gggtattaaa atgaagaaaa aacgttttgt
     5341 aactggattt gccgtattag cattttcagc tctagtgtta ggcgcttgcg gcgcagatga
     5401 caacggaagc tcaaactctt cttctgaatc atctacagca caatcttcga ctgcaaaaag
     5461 cacaacagaa tcatctgcaa aagttgtagc tggcggagat ttgcaagacg gcacatataa
     5521 attagaagaa aaaaattatt caaatggtta ccgtgcagtc tttgaaatgg ttgtaaaaga
     5581 cggcaaaatc actgaatcaa aatatgataa cgtcaatgaa aacggcgaat caaaaacaaa
     5641 agatgcagaa tacaacaaaa acatggaagc aaaatctggc acaaacccag aaaaatacat
     5701 tcctgagttg aatgaccaat tcttaaaagc acaaagcgca agcggtgtgg aagtcgtaac
     5761 tggagctact cactcatctg aaagcttcca aaactacgca caacaattga tccaagctgc
     5821 tcaagctggt aacacagata caatcgaaat cgacaatggt gctgatttga aagacggcac
     5881 ttacaagctt gaagaaaaga actactctaa cggctaccgt gtgcaattcg aaatgacagt
     5941 tgctggcggc aaagtaactg aatcaaactt tgattatatc gacaaagacg gtaaatcaaa
     6001 acaagatgat acagaatata atgaaaatat gaaagcaaaa agcggtacag aaccaaaaac
     6061 ttacatccct actctgaacg acgaattcgt caaagcaatg ggcgaagaag atggctcacc
     6121 tgctaatgta gaagttgtta caggtgctac acacagctca cattcattca tcatgtacgc
     6181 acaacaatta gtgaatgcag ctgaaaaagg cgacactcaa acaatcgaag tagataatat
     6241 cgtaacagaa aaataatcac ataatcatac taataaaagt tggagggtgg gacgatagcc
     6301 agattaggca gtcgtctcac ctttcaaaat gaagagagga aaaagatgcg gaaaaaacta
     6361 ctattttcct taatcacact gggcatctct tttatggtgc taactggctg cagcaactca
     6421 gcaaataaaa caaccactga aagttcagca gctaccaaga tattgaaaga tccatattct
     6481 gatgaacagt tcttgttagg gacttacgtt cgtatccgtg tgtatgatga aggcaaagaa
     6541 tcagcattga aacctgcctt cgatcgagta aaagaacttg gtgataaaat cacgatcaat
     6601 caaaaaggtt ctgagatcga tgaagtgaat gagcaagctg ggatcaaacc agtaaaagtt
     6661 tctgatgacg tctacacgtt agtcaagcgg gcctacgaat atagccaaga ttctcaaggc
     6721 ggattcgata tggcgattgg tgcaatcaca caattatggc gaatcggttt tgatgacgcc
     6781 agaaaacctt cccaagaaga aatcgatcag gcattgaaat tagtggatta tcataagatc
     6841 gaactaaatg acaaagaaaa aactgtttat ctaaaagaaa aaggaatgat catcgattta
     6901 ggtgcaatcg ccaaaggtta cattacggac gaagtagtga aagtcttgaa aaaacaagga
     6961 gtgaccactg ccatcgttga cttgggcggt aatgtctatg tcttaggcca cagcccaaga
     7021 ggcgaaaacc aagactggac agtcggcatc caagatccga acatggctcg cggatctgtt
     7081 ttaggttcta ttaaagaacg gaacaaaaca ttagttacct ctggaattta cgagcgttat
     7141 ttagaagtag atggcaaaaa ataccatcat ttgtttgatc caaaaacggg ttatccgttc
     7201 gacaatgata tcgcaagtgt cacgatcata acagataaat caattgatgg tgacgggttg
     7261 tctacagcag tcttttcaat gggtgtgaaa aaaggactgg aatacgtcga aagcgaattg
     7321 aataacggga cggaagcgat cttcgtgaca aaagacgata aagtatatgt gaccgatcca
     7381 atcaaagaca ccttcaaatt atcggaggac tcccattaca cgatgggtga ccgttctgaa
     7441 ctaaaataag gagtacgaac catgacacta aaaatctttt tagaagtagt tgaaatacga
     7501 acaaaggtgg ctagtgtatt tccttttgcg gtaggggtat tattctctat cgcttttttt
     7561 catgagatac attggctaaa cactttttta ttttttattg gaatgctagt ttttgattta
     7621 gcgactaccg ctatcaataa ttacatggat tatgaaaagg ctcattctga agtatataag
     7681 tatgaagaaa atgtcattgg ccgcagaaat ctatctcctg tattgattcg aaacatgatt
     7741 ttagctatga ttgcatttgt ttttgttatc ggcgttattc taacgatcaa tacgggttgg
     7801 cttttacttt tgatgggatt tatctgttgt tttattggag tattctatac attcggaccg
     7861 attcctttat ctcgaatgcc tttaggggaa attttcagtg gggtgactat gggccttgga
     7921 atttttgcca tggtcgttta tgtcaataca ttcggttcag atgtctatga tctgacagtc
     7981 aacttttcat ctggacaatt tgctttggta ggaaatcttt gggctatagc agcaatcgta
     8041 ttagcttcct tacccttggt gtttacgatt gccaatatca tgttggctaa taatttgcgg
     8101 gatcttgacc gtgatattga aaatcatcgc tatacactag ttttttatat cggacgtcct
     8161 gcgggaacag tcttattcca gctattgatg tatgcttgct atttcgtgat tttaatgggg
     8221 ttgttgagcg gtatctatga atggccgatc ttggtcacct tcttgacctt gccaattgtg
     8281 aagaagcatt tggatcaatt caagcaaacg ttaccccagc ctatcagttt cagctattcc
     8341 atcaaaaata tggtcttatt caatgccagt tatgtattgg ggctattact aacaatcatt
     8401 atgcaagcca tttaacagca tgaagaggtt gtgaaaaaac tgccctgcat caagtaataa
     8461 gaacagccaa ataaaaatag ctccttatat ttttcgtttg gttgggctta ttaccgcaga
     8521 tgcaagcttt tgaacaccgt ttatcaggag gttgtgaaaa agtcgcttag tttcgagtat
     8581 taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga
     8641 gaaactggct tttgaacacc gtttatcaga ggttgtaaaa tcagtgtttt gtcctgagta
     8701 ttagagaaac aaatggaaaa atagttcctc atattttgcc gttttttgag ctaataccga
     8761 aggacagctg attgaacact atttgttcgg aaaaagtgac taactatgac acaatttttt
     8821 tcacagtccc ttttttcact tgatttgatt cgtaccagcg aacttgacga gaactttctt
     8881 ggtttggtaa ctatatggta ttcttttaac atcctcgttt gcttcttcta atttatttat
     8941 acaaagtcac acttttttaa acatctgttc ttcgatacat ttctatttct tttaaacatc
     9001 gataccaata ttttgaactt aagagtgcat accaaatgga ttcatttact cttcgtcagt
     9061 tacgaagata atcaaaatct ccaacttccc tacttatttc gttttgagaa ttatcgaagc
     9121 aatcttacat aaataactaa aaaacaaaaa accagaaaat cagtatactg attttctggt
     9181 tttacatggt ttgttcaata aaaagctgtt ggaactgtta atattaaatt atcgcatcgt
     9241 tacgaattct tccgctcctg ttggatggat ggctactgta ttgtcaaagt cttctttagt
     9301 tgcacccatc ttgatagcca cggcaaaacc ttgcagcatc tcatcgacac caacaccaat
     9361 agcatgtaag ccgacgattc tttcttcttt tccgacacag atcaatttca tttcgcattt
     9421 ttggcgataa tcatttaatg caaagtacat tggggtaaag cgtgaacgat aaactttcaa
     9481 ttcattttcg ccatattttt ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg
     9541 tggatgcgta aagacgactg tcggaacaag tttatagtca agataagaat ctttctttcc
     9601 attaaataaa cgttctgata aacgtctacc agctgcgatt gcaacaggag tcagatctaa
     9661 tttaccgatc acatcaccaa cagcaaaaat atgttcatcc gttgtatttt ggtatttatc
     9721 tactttgata aacccttttt tgtccaattc cacatttgta ttttccagac ctaatttttc
     9781 ggtattcggt acacgtccac cggcaaaaag tacacaatca ccagttaagg tttcaccatt
     9841 ttcaaatagc accgtatatt cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc
     9901 gtaagttttt attccagctt cttggtacat ttggatcaaa ttgtccgaaa gcattttatc
     9961 aaacgttcta agtggtcgtt cttttcgata tgcccacatg acttcactac ctaaaccacg
    10021 aaatacacct gaaagttcag ccgcaatgta tccagcacct aaaacgattg ctctcttagg
    10081 taactctgtt aattcaaaaa atccatttga atcaattgcg tattctccac caggaatggt
    10141 cattttactt gcacggcctc cagtagcgat caagatttta ggagcacgat aggtttcacc
    10201 gtttacttcg atcgtttgac tatcgataaa agtagcgtaa ccttttatag cctcgacatg
    10261 attgctatct aatcctcttt gataagcacc gtgtagaaaa tcaatatatt tttctcgtct
    10321 ttctactaga ccggcgaaat caacattttt taaatctgct tggataccgt aacttgaggc
    10381 atctcgttca attgtttcca aaatcgtact tgcttgccac ataacttttt taggtacaca
    10441 accgacgttg acacatgttc cgcccaattc attcgcttca atcagcaaaa cttttgcgcc
    10501 atgcatccct gctctgtttg ctgaagcaat accgccagag ccgccaccaa ttacgatgta
    10561 atcatatatt tgcattttgt tcctccttat tgaagagatc aatcaaaacc tttatttaac
    10621 gatccttttt ctacagaaaa caagtgtaac acttttcttg aattttgtat gcgaagatgc
    10681 ttaaaacagg gatatatcag ctttttcctt atttacgttt tcggatcata tctccaggtt
    10741 tcaccggctg tgcgactggc atagttaaaa tcatcattgg atttggtgct cggtcaatgg
    10801 attcgccttc ttcattatgc ataacttcaa cggtttgact gaaatgactg aatcctggac
    10861 cataaaattc gatttcatct cctacagaaa acaagtttct ctgacgtatc gtagcaatct
    10921 gtgttgcctc atcataagat aatacttcac cgacaaattt atatactggg attttacgac
    10981 gttcaccgaa taattgttcg ttttcagttg gtgtattgta gtaaaaacct gtagctaatt
    11041 ctcgttgtgc tactttccat aactcgtcga tccattcttg cttgcagaca tagttttcag
    11101 gatcttccat ataactgtct acagccgctt tatagacatt tgacaccgta gatacataat
    11161 ggatcgattt cattcttcct tcgatcttga agctgtctac gccgttttca atcaattctg
    11221 gaatatgttc gatcattgac atatccactg cactcatcga gaattcttct tccacttcgc
    11281 ctgtatcagt taaactgttt cgttcttgac caaaaggcat atcgaacaat ccatacttcc
    11341 agcgacaaga ttgagaacat ccgccacggt ttgcatcccg catcgacata tggttggaca
    11401 atgtgcatcg accagaataa gagatacaca tggcaccatg gataaatgct tcgatttcgg
    11461 tatccgtatt tttacgaatc tctgccactt cctccattga aacttcacga gctaaaacga
    11521 cacgttccaa cccttcttct ttccagaatt ccaatgtctc aaaattagtt gctgaagctt
    11581 gagtggataa atggatcggc agacctgggg cttctgtcgc acaaatttcg atcaaagctg
    11641 gatcagaaac aatcactgca gaaataccga catcacgcaa actgcggaaa aattcccctg
    11701 ctccatcttc atttccttca tgggtgacca tattagcagc aacgtatacc ttcgcatcat
    11761 gttcttttgc aaaagcaact ccttcagcca tttcttcata agtaaaatta cctgcacggc
    11821 ttcttaaacc atatgcattc ccgccaatat atactgcatc tgctccataa tggatcgcgg
    11881 tttttaactt ttccagtgtt cctgctggcg ctaggatttc tggtcgtttc aattttcttt
    11941 ttactgtcat ttctttcatc atactcctta tttgatagca gaaggatcaa tataaaagaa
    12001 tcctgtatct aattcacggt tttcaggatg caatttactg atttcgctag tcattttttc
    12061 cgctttttct tgatcccagc taccttctaa aatttgtttt tttgcttcgg cgaatagtcg
    12121 agtgatgttt acgaattttt cgcctggagt aaataatccg tccaatttcc atgtacagta
    12181 atgatgctga taaagttcat tcaattcatt cgagagattc agatcattgc tggcaaaaat
    12241 atgtgtgcca tgactatctt cataaataga ataatgtgtc tcttcttttt taggttccga
    12301 gataaaaaga cctctttcac gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt
    12361 ctgtagcagt ggacgtttgg attgatggat gcatgtagca ccatacacaa gggtttctac
    12421 aggaatatcg agtttttctt ccatcgcctt catctcttca aaaggaactt cacgagcaag
    12481 tactgctcca actgcgcctt ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa
    12541 tgtttcagca tcataaatat acggtatacg caattcagga ttttttttca tcgtataaat
    12601 aatacctgta tcaccaactg tgatcacatc tacgtttatt tcttttaaaa aagccaaata
    12661 ttcaggtaca tttttcattt tttcaggatg aatgatccca tttacagcaa tattcgcttt
    12721 tttaccatac ttgtgtgcta attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc
    12781 aggcagacgc aatccaaatt gctcttcacc gaaatatagt gtatctgcac ctgcagcaag
    12841 caactgttct gcttgttcaa tagtttctac tgtggttatg agttcgatca taattttctc
    12901 ctcctacaac ttcataatag tagcatagct ctaaaaactt tccaatactt cttctattta
    12961 aagtcggatt tttgaaaatt atttcacaaa aaatcaaaat aaagttcgat atattctgtc
    13021 caaatataaa aagagattgt gacaaaaatc ttgtcacaac ctcttaatca agtcttacta
    13081 ttataaaaat tcagctacac aggccaagta actataggga acctactgtt tcctttttag
    13141 gtccgcgaac attcaatgtg atcttacctt ttgaagcacc tactgttacg tggtcaccta
    13201 aacgaatttc tccgccaagc aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg
    13261 cgcgacggat tggacgtgca ccatattcag gatcaaatcc agctttcccg atcacatcga
    13321 ttgcagatgg tgtgattttc acttgaatat cttgttcttt caatcgtttg ataatcgctt
    13381 tgctcatgat ttggacgatt gtatggattt catctttact caatgaatgg aagacgacag
    13441 tctcatcgat tcgattcaag aattcaggtc ggaaagcttt ctttaactct tctaagatac
    13501 gtttttgcat ggctttgtga tctttagtaa catcttggac attgaatccg acattttttt
    13561 cttctcgaat ttgtgtcgct ccgatatttg aagtcataat catgattgta tttctaaagt
    13621 ccacttttct acctttagaa tctgttaaat gaccatcatc caatacttgc agaagtaaat
    13681 tgaaaacgtc tggatgtgct ttctccactt catctaagag aatcactgaa tatggttttt
    13741 gacgaatttt ttctgtcaac tgtccacctt catcataccc tacgtatcct ggaggagacc
    13801 cgattaatcg gcttgtactg tatttttcca taaattctga catatcaaca cggatcaggg
    13861 catcttcact tccaaacatg acttctgaaa gtgctttggc caattctgtt tttccgacac
    13921 cagttggtcc aaggaacatg aatgaaccaa ttggacgatc aggatctttc aatccgcttc
    13981 gtgcacggcg gatcgcacgt gaaactgctt tgacagcctc atcttgacca acgacacgtt
    14041 catgcagaag tccttccagt tctaacaagc gttcgctttc ttttttctcc aactgctgta
    14101 gagggacacc agtccactcc gatacaactg tagcaacatc ttcttctgtt acacgatcag
    14161 aataaccttg ctcctgtttt gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg
    14221 ctttttcttg tctgcgtaat tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt
    14281 ccttttcttg tactagttca tcgatttcta gtttgatcac agttgatttt gttaagtgat
    14341 cggtttgatc caaacggact ttcgctgctg attcatcgat caaatcaatt gctttatcgg
    14401 gtagttgacg agaagtgata taacgaacag aaagattgac tgctgctcgt acagcttcat
    14461 ctgtgatctc cactccatgg tgctcttcat atcttgtacg taatccttga agaatcactt
    14521 ctgcttcttc aggagttggc tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag
    14581 aatctttttc gatatatttt tgatattcat ctagggtcgt tgctccgatc gtttgtaatt
    14641 ctcctcgagc taacgccggt ttcaagatat tcgaagcatc aattgctcct tctgctccac
    14701 ctgcaccaat taaggtatgc aattcatcaa tgaataagat tacttgacca tcattataga
    14761 tttcgtcgat gacctttttc attcgatctt caaattcacc gcgatatttc gtaccagcaa
    14821 ccaaagatcc catatcaagc atcatcaggc gcttttcttg catgtcttct ggcacttcac
    14881 ctaaaatgat tttttgagct aacccttcag caatggctgt tttgcctaca ccaggttcac
    14941 ccaccagtac agggttattc tttgtccgtc ggctaagaat ctggataagc cgtttcactt
    15001 ctctcgaacg accaacaact gggtccagct tgttttcttt tgctaatttc gtcaaatcac
    15061 gtgctaatga atctagcgta ggtgttcctt gcggtgtttg ccgtttatct tgtacattta
    15121 catttctacg acgattcatt ccttttccag cagaatcacc agctagcccc attttttgtt
    15181 tcagaacttg acgggctttt gaaaggctga tacctaagtt gatcatgatt cgagtagcca
    15241 tgatactttc atcttttaat aagctcatca aaatatgttc tgttccaact ttttgagaac
    15301 cagttttctt cacttcgctg ctagctaaag caaaaacttg tttcattctt ggtgaatatg
    15361 gaagaaagac tcctttaggg taagaacgga taccaccata accaatcaga tgttcaattt
    15421 cttcataaat ttccgtttca ttcaaatcca tttcacgtaa tacttttcca gcaattccat
    15481 tttgctcgac cactaaggct agtaaaacat gttctgaact aactgattga tgtttcaacc
    15541 gttttgcttc ttcttgtgca atttctaata ctgcttgggc tctttcagta aaaagttctt
    15601 ccatctaatc tcacttccca tcttcataac ttaaacgttc cagaaatgct cggagaatcc
    15661 gagcacgtgt atgatcttca tattcattga aattcaatgt gttttttgca attgcactca
    15721 acatcaagtt tccttctttt tttgtaatta tttggtcttc ataaagtttt tgaataatag
    15781 caaaagcatt tttctcactg attgtatcat caaataactg atttatctgc tcaagtaatt
    15841 gttttttatc tgaaatcctt acttttgcaa ttcgtatata accaccacca ccacgtttgc
    15901 tttctactag ataaccacgt tggatggtaa aacgggtatt aatcacatag ttgatttgtg
    15961 aaggaacaca gttaaagagg tctgccattt caatccttcg aatctcgatc atttcactat
    16021 tttccagaat tttctttata tatgattcaa ttaaatccga ggtattctga tgtgccatct
    16081 tattcattcc ctttgactaa tattgacctt aattataacg aatttgataa tagaaagaaa
    16141 gaagaacgct ttttgttaaa taaatccttt agaatcaaca aaaaaaaata cggaatatac
    16201 tattccgtat c
//
LOCUS       DAYYAR010000056        16052 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000056 DAYYAR010000000
VERSION     DAYYAR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.056"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACHAT0_001558"
     CDS             complement(51..1598)
                     /locus_tag="ACHAT0_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8451502.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHAT0_001559"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHAT0_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8451503.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="ACHAT0_001560"
     CDS             complement(2043..2237)
                     /locus_tag="ACHAT0_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451504.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(2463..3665)
                     /locus_tag="ACHAT0_001561"
     CDS             complement(2463..3665)
                     /locus_tag="ACHAT0_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8451505.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(3668..3862)
                     /locus_tag="ACHAT0_001562"
     CDS             complement(3668..3862)
                     /locus_tag="ACHAT0_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8451506.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(3909..4757)
                     /locus_tag="ACHAT0_001563"
     CDS             complement(3909..4757)
                     /locus_tag="ACHAT0_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM8451507.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(4962..5312)
                     /locus_tag="ACHAT0_001564"
     CDS             complement(4962..5312)
                     /locus_tag="ACHAT0_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451508.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            5490..6353
                     /locus_tag="ACHAT0_001565"
     CDS             5490..6353
                     /locus_tag="ACHAT0_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8451509.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            6977..7969
                     /locus_tag="ACHAT0_001566"
     CDS             6977..7969
                     /locus_tag="ACHAT0_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8451510.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            7985..8869
                     /locus_tag="ACHAT0_001567"
     CDS             7985..8869
                     /locus_tag="ACHAT0_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8451511.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            8918..10525
                     /locus_tag="ACHAT0_001568"
     CDS             8918..10525
                     /locus_tag="ACHAT0_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8451512.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            10526..12010
                     /locus_tag="ACHAT0_001569"
     CDS             10526..12010
                     /locus_tag="ACHAT0_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8451513.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            12187..13179
                     /locus_tag="ACHAT0_001570"
     CDS             12187..13179
                     /locus_tag="ACHAT0_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8451514.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            13231..14100
                     /locus_tag="ACHAT0_001571"
     CDS             13231..14100
                     /locus_tag="ACHAT0_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8451515.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(14520..>16052)
                     /gene="guaA"
                     /locus_tag="ACHAT0_001572"
     CDS             complement(14520..>16052)
                     /gene="guaA"
                     /locus_tag="ACHAT0_001572"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8451516.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4863 a         2804 c         3249 g         5136 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttacg ttgaaaaacg ttgatttatc
     2341 ggcattcccc atccatatta tatttacgtc ttattcccac tcaatatgag tagcctcgta
     2401 aagaagctat tatatcagtg ctttttcagc ctattcctca ttttggtagg gaaatggtag
     2461 ggttagattt ttactgcttg atttccatta aagtttgcaa ctttagattt tgttgcacag
     2521 gtaaatttaa tgtttcctgt catttgttct gcaattcctt catcattacg gctccataga
     2581 tgagcgtagt gctttaaagt gatttcaggg ctagaatggc ctaaccgttg cgacacgacc
     2641 agaatgtctg cgttaaactc attgattagg tagctaacat gagaatgacg taatcctttt
     2701 ccttgtatta ggggtacctt agctagtttg gcataacgct gaataattcg ttgaacagtt
     2761 gaacgataga gtgggaggtc agtgtagcta agaataaatt gttctacacc atgatttttt
     2821 tgaataactt tccacttttt tagcacttca attgtatcat cgtccagaga aatgatccgc
     2881 aagccgcttt cagtttttgt gtaaggtttc cgaataaaat ctttttgatt tgtcatttcc
     2941 aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca cgtcattcca attaagtgct
     3001 aatccttcgc ttacacgaac accagtcatg taatatagcc agatcattaa aaaacacata
     3061 tgttcgtaga aatcatcttt ataaattttt gccaaaactt cttcaaattc ttcttttgtc
     3121 cagtaaggaa cgattgattt tccttttgga attgcttttg ttcttttgga aatattctca
     3181 ttcagaaatt gcagggtgac cgcatagtct aatgatttac gaaacatacc gtacataagt
     3241 gaactgtatg cttgtgagta tccgctatcg ttcaataacc aaattcgata attttcacaa
     3301 tctcgcacat tgatttcacg aagcttctta tcaccgaaaa attcagtaat ctgaatcaat
     3361 ccacactcac gagaactcca agtacttttt tgtacgctag ctttgtagtg tggaacgtaa
     3421 gactgctcca taaattcttt gtaagttaaa tcataattcg caaatccatt tacaaccatg
     3481 tattcatttt ttatacgcat gacttctttg tgtgcttcac gagctgatga gaatcgtttt
     3541 cctagagaat cttttgttcc tttcttctgg atcctctttc cagtcacttg atctgttcct
     3601 aactcaacta gataagaaaa gcgaccattt ttatcttgat acacacaagg gtattttgtt
     3661 ttcatagtta ttcctcctgt gaaagaggaa cacctataat ttcttcaatt acttgtcggg
     3721 gtactacccc aactctttta ttattataat atggatatcc ctgatttacc attcttgttt
     3781 ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa tcctagagcc tctaaatctt
     3841 ttcgacaaat tgtatttttc attgtataaa ctcactttca aagaaaacac tatcattggg
     3901 taatagtgtt atttctttat ttttgtataa tgaacggtac tttcttgaaa atccatcact
     3961 tgttcaggac ttcccaactc atttaaagct aaagcattgt aaaaaaatgg acgtctaact
     4021 atatattcgc ttgtatatag tatatcttga ttcgtggaat catagggttt ctttaccatc
     4081 tttttggtga cagttggtag tttgagattc cgggacttga aaaaagcttt tttcttatgc
     4141 tctttcaatt ccagctcttt atcgaaatat ttgctgatat agcgcccgcg attttcgata
     4201 ctgtctacat cgatcttgtt gatcttaacg aaaccctgac cccaaatcgt agttaaccgg
     4261 ttaaatgaga taaaggggaa atcaaacaag ataatatggt agtggattgc accgcgttgc
     4321 tgcttttccc acgtggcgag atactttaaa tttgcttttt tagttttata gaggtcataa
     4381 ttcagtcgtt tgataaattt tttaaattca ttatttgcat actcaatgtc ttgaatgttt
     4441 tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa aattcgtgtc tacaaggcgg
     4501 gcaatctcaa atcgcatact ttgataatgt ttctgtttgc gttttaaact gtcatattgt
     4561 ccttgagcgg ataattcatc atagtttttt cttttttcaa gagtaatatc attttcataa
     4621 atccaagcta atttttgttt tcgttccaga tttacttcgt tttcgatcgt tttacgtttg
     4681 ggagtaatga ttgcttcata atcataaatt tcaatatacg tgggagtttc aatgattttc
     4741 tgattataag cttgcaaaaa tcatcactcc ttgttgcgta ttcgattgtc aaagaaccag
     4801 tcattttctc ctgatgttga tctataaatc aagttaaggg ggaaggcctg cgtagcagcc
     4861 cttctagcct atggggtgtt gggcggtctt aacggacaga aacggctctg tgggcgctgc
     4921 cccgccgttt cgtcctaacc acccaacact ccatagtata gttagttttg tgtagtggta
     4981 ccttgttttc tgggaatcaa agttgccgta tcagcggttg ccgataaccc ataactgaca
     5041 aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat tggtaaatgt aattatggct
     5101 aaaccttgtt gggtaagttc ttccggcgtg atcacaggat gttcagattt gactttgaca
     5161 ctgatttttt cgtagcgaag ttttggtagg atacattcgt agctccaacc tagaatttcg
     5221 ccctcctttt gccagcgagt gacattgacg acttggtatt ctttttcgag tgtttctttt
     5281 ggtaaactga gatcggtaat ttttattggc atcacaattc tcctttttta tacgaaagtt
     5341 tatacgattt gattaaaaaa tatagatggc catctacgac ttgtattata tacgagaact
     5401 tatacgtagt caagagattt tctaaaaaaa tatatacgat tttttatacg agtgttataa
     5461 tgaaaaaaat gataaggagg aatttgaata tgtttggttt agtacaacct aataaagtga
     5521 aggtgggcca acgaatcaaa gaaataaaag acggaatgtc tctctctttt acagaattag
     5581 ggaatcgatt aggtttaaaa aagccgacga tcagttccta cgttcaaggc tatgcgttgg
     5641 ctcctaagag tgtgatcaac caattatcta gtatcagcgg aaaacctgtc ggctggtttt
     5701 attttgggca tgtggaagaa tatatccgag agtatttgtt tctcattggt cagaaaaaaa
     5761 tagtggacga ttatcctgaa atcattgaga aaataaagca agaattttat acgggcgagt
     5821 tcaaaaatcc tgcttgggag aatgagctag gctatccttt ggaggaattt atccaagaat
     5881 gttttgtaga atattcgagt gaaatcatga aggactatgt cgtacagatt acgaaagagg
     5941 tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa agcaaaagaa gaagctgtca
     6001 cgattctttc atctgggatc attgactata tcgagatgag tggtgagatc aagtatggag
     6061 agaaagatcg cattgcgaaa atggtgaaag aatcaataaa aacctttgat gtgaaacaag
     6121 acctgacatt taatgattcc tatttgattg gaaagttgat caatattctt ggtacagatg
     6181 aagatacgga gctgtttctt tcttctttat caatgatatt tactgataag catttttcca
     6241 ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc gatgcgacca gcgttaatca
     6301 aactctatcg ggagaaaaca ccagatgaat tttatgattg gtttgagaag taaaataaga
     6361 aaagtggctt agattacaag ttggtgaaaa aatttttttc agcataacat cagtaatcta
     6421 taaaggggtt gttgattata ctgtagaagt taagtactca agagattttt tgtacgtaaa
     6481 caaagcaaaa aagggtgcag tttcactaga aaatgtactc ttttttgtga tttttcttag
     6541 ttatagagtt tttttgaaac tttaaaaatg agaaaacata ttgattttaa agagtgtaga
     6601 catagcttct tttgctaaat agattaaact tactggtgag tggtcttgaa catactttta
     6661 aatcacattt tactgagttt tattggattt tataattcat tggaaacctc gtacttaatc
     6721 tgtttttatc tatcgactag aatctgtata tattccagct atcaatgagg ccgcagaaaa
     6781 gggtgcttaa aaataatctg ttgtttaaaa tattattatc agaatactta gatacaaaat
     6841 tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg atatggtaat ctataaacga
     6901 aaatagtggt aacgtttcca aaatgcatct tcgttaaatt tttatttttt taaacgaaaa
     6961 tggaaacgtt tctaaaatgt attgggagga gattgaattg gaaaagacat tgggaccagg
     7021 tggtgagata agggcggtac aacgggataa aaaaaatatt tcgaagtggg aaaaattaaa
     7081 gaaacagttg cggcgtgata agtggatgta cttgttgctc ttaccaggta tcatcatgac
     7141 gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt gcatttaaag actataatcc
     7201 tatgctagga attttggaaa gtccttgggt aggactagat cactttaggg aatttatcag
     7261 ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa attagtattt atggtttatt
     7321 gttaggtttt tttccaccaa ttattttagc actgagcttg aatcaaatta tcaataaaaa
     7381 aataaaacag aaaatccagt tgattctata tgcgccgaat tttatctcag tagttattat
     7441 tgtggggatg ctgtttttgt tcctatccgc aaatggacct attaatgggt tagtagaaaa
     7501 ggttacgggg caaccaatta tgttcatgtc taatccagct tatttcagaa cgatttatat
     7561 tttatcggga atttggcaag gaatcggctg gtcatccatt ctttacaccg caacgctggc
     7621 gggagtcagt accgatttat atgaggcagc aaatatcgat ggggcaaata ttttacaaaa
     7681 gatttggcat atcgatttgc ctgcattgag gcctgtgatg gtgatcagct tcatcttggc
     7741 cgccggcggt attatgaatg taggatatga aaaagcctac ttgatgcaaa cttcaatgaa
     7801 catccctgcc tcagaaatca tttccactta tgtatacaaa gtcggcttgc aatcaggtga
     7861 ttatgcctac tctgctgctg ttggtctgtt caatatgatt attaatgtcg ttttattact
     7921 gattgtcaac aatataacca agcggctaaa cgatggtcaa ggcttgtaat gaaaggagaa
     7981 aattatgaat agtgctttat caaaaatcga ccgtcaaatc ttatggttga atcgagtgat
     8041 tgtaactttt ttagtcgtca tcaccattgt tccgctgatt tatgtactgg tagcttcttt
     8101 tatggaccca gctactttga tcaataaagg tataagcttc aatccgaaag attggacaat
     8161 acagggatat caacgggtat ttgcagatga ttccatttta cgcggttttg taaattcgtt
     8221 gttttactct tttagcttca gcttattgac tgtatttatt acgatgatca ctgcgtatcc
     8281 tttgtctcga cctaacttgg cggggaaaaa gattattatg actttttttg tcattacaat
     8341 gttcgtgggt ggtggcttga tacctaccta tttattggta aagaacttgg gcatgttaga
     8401 tagtgtatgg gcgattatcg taccaggtgc tataaatgtg tggaatatta tcttagcccg
     8461 tacctatttt agtgctttgc caaaagaact ttctgaagct gctactatcg atggcgccaa
     8521 tgacttgcaa atactgttca aagtcatgct tccattagcg aaaccaatta tgttcgtatt
     8581 gtttttatat gcctttgttg ggcaatggaa ctcatacttt gatgccatga tttacatcaa
     8641 agatcccgac ttacagccgt tgcaactcgt attaagaaat attttgatcc aaaaccaacc
     8701 ttcacaagac atggtgggag ccaatactgc catggcagaa atgaaacaaa ttgctgagtt
     8761 gatcaaatac gcaacgatcg tcatttctag tctgccatta atcataatgt atcctttctt
     8821 ccaaaaatat tttgataaag gtgttttagt tggttctgtt aaagggtaag tattgttcac
     8881 tatttgtatg aataaaaatt ataaaggaga ttttaagatg aaagcaaaat ggatgctgcc
     8941 aatcgttttt acttccgtac taattttggg ggcttgtgga ggctcaaaag aaaagagtaa
     9001 atcaagccct gattatgaac tagaaaatgt ctcttttcca ttgaaagaaa aagtatcttt
     9061 aaaaatgatg tctcaaagtg cccctttggc tcccaatgat cctaatgaca aattgatttt
     9121 ccaacgatta gaagaagaga ccaatctcca tattgattgg acaaactaca gtagtgattt
     9181 tgccgaaaaa agaaatttgg atatcgctag tggtgattta ccagatgcca tcttcaacgc
     9241 cgctgcaggt gactatgatt tattaaactg ggcggaaagt ggcgttattg taccgattga
     9301 agatttgatc gaagattata tgccaaatct gaagaaaatt tttgatgaaa accccgaata
     9361 ccgacaaatg tctactgcac ctgatggtca catttactct atgccttgga ttgaagaatt
     9421 aggcgaagga aaagagtcga ttcatacagt aaatgggatg gcttggatca acaaggagtg
     9481 gttggataat ttgggtcttg aaatgccgca aaccactgat cagttgatga ctgtgttgga
     9541 ggctttcaag acgcaagatc caaatggtaa tggtgaagca gatgaaattc cttttagctt
     9601 tattaacaat ggaggaaacg aagacttaaa atttttaatg ggggcattcg gtctaggtga
     9661 taatgatgac cacttagtcg ttgataacga tggtcaggta caatttactg ctaacaaaga
     9721 agggtataaa gaagccatca aatatttcaa tgaaatgaat aataaaggtc ttattgataa
     9781 agaggctttc gaacaagatt ggaatacgta tgttgccaaa gggaaagaac ataagtatgg
     9841 cgtgtacttt acttgggata aagcaaatat cacgggtgca gatgatagtt atgacgtctt
     9901 gccagtctta gcgggaccag atggaacgaa acatgttact cgtacgaatg gcatgggttt
     9961 ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac ttggaactta cagctaggtg
    10021 gatagataaa atgtatgaac cgttacagtc tgtccaaaat aactggggaa cctacggtga
    10081 tgaagcacag gcgaatattt ttgaattaaa ggatggtatg ttaaaacatt tgcctttaga
    10141 ggggacagca ccgggggaac taagacaaaa aaccgaagct ggaggaccat tagcagttct
    10201 tgattcttat tacggcacag tcactactat gccagatgat gcgaagtggc gtttagattt
    10261 gatgcatgac acgtatttgc catatattac agaagacaat atctatccac gtgtcttttt
    10321 agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg gatatgtggg attacatcta
    10381 tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat gaagaatggg aagaatacct
    10441 tgctgaattg gaacgtgtag gtttggaaga atggattcaa ataaaacaaa ctggctatga
    10501 taattttttg aagggagcac aataagtgtt agtagaagaa acggcgattt tggataaagc
    10561 aaatcgattt attgaaatga tgcgaggaaa agttaaccct cagtatcgac aaaactatca
    10621 tttttccgca cccatcgggt ggataaatga ccccaatggg tttatatatt acaaagggca
    10681 ttaccatctc ttctatcaat actatcctta cgatagtcga tgggggccca tgcattgggg
    10741 gcatgcgcgc agtagtgatt tggtccattg ggaagatcta ccagtagcgt tagccccaga
    10801 tcaaccttat gacagcggcg ggtgtttctc aggaagtgca attgaaagag acggaaaact
    10861 atatttattt tatacaggct gtcaagaaat tgatggtatt agccatcagc atcaatgttt
    10921 ggcggtctct gaagatggta ttcattttag caaatacagt ggtaatcctg tggtagcaga
    10981 aaaacagcta ggtgaaaatg ggctaataca agaatttcgt gatcctaaag taattgttca
    11041 caacgatggt tactatatgg tagtagctac taaatctaca cagtctactg gtagaatagt
    11101 gatgtttcaa tcgtcagatt tgtatacttg gcacttttac tctgtgctgc tagaagggac
    11161 ttcagagcaa ggaataatgt gggaatgtcc tgatttattc catttagacg gacgagatgt
    11221 cttaatcatg tctccgatac aaatcaagaa gaacggactg gaatatcata atatcagttc
    11281 taccatggct tgtattggtg aggtaaactg ggaaacaggt cagatgatag ttgataattt
    11341 ccatgaaatg gattttggaa tggattttta tgcaccacaa acaacaagag atcctaatgg
    11401 tggacgggtg atgattgctt ggatgcagat gtgggatcgt acattgccta cacatgagtt
    11461 aggccatctt tgggcaggct ctatgagttt gccccgagag ttaagagtga aaaaaaatcg
    11521 attggtacaa aaacccatct caactgtata tagtcattta gtttatcaac aaggcagtga
    11581 aaatattgca gtgactgaca catcagtcgt atttcgaaac gcgctgtctg attcttcata
    11641 tttacatcta gtcgcagatt tggagaaagc tgaagttttt acaattgaat tactgcggtc
    11701 taagacggat tgtctcaaac tagcctatga cactgctaca caggtttttg aattggatcg
    11761 tcagaaacta ggaagagagc ttacaggggc ggaagaagac cctctgaatg tacgaaaagt
    11821 tgaagtacct ttgattgata atcagctcgt tcttgagatt tttagagata cctcatcaat
    11881 tgaagtcttt attaatgggt tagaagttat gacggctact ttctatgagc tggaaaaagc
    11941 tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt caagcttttg aaacagcgag
    12001 tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt atttaatagg ttttgaagga
    12061 taatgttttt agaaaagaac agatagctag atttcccatg gatttgctat tagtattttg
    12121 aaaaaattag taatttatga caaaattcat tatactaaac ttgtgcagaa atgtgaggga
    12181 tcaactatga aagcaacaat gaaagatgtc gcaaaattag caggtgttgg agtaggaact
    12241 gtttcccggg tattgaataa cggttctgtt aaagcttcta ctagagaaaa agttgaagca
    12301 gcaatgacag agttaaacta tcaaccagat ttttatgccc gggggttaaa gatgaagcat
    12361 acgaacacgg ttgctttgat tctacccacg gtatggcatc cattttttgg cgaatttgcc
    12421 taccatgtag aaacagcatt agaaagacaa aactacaagc tttatttgtg taactccagt
    12481 ggtaatagtg aaaaagaaca agaatatatc cagatggtca gccaaaataa agtagacggc
    12541 atcattggaa ttacttattc agatatcgat gaatacattt cgagtaatct gccttttgtt
    12601 agtattgacc gttatttcac tgaagaggtg atttatgtga ccgctgacaa tgcggccggt
    12661 ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc agaatgtagc atatatcggt
    12721 gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa aatattttga aatcgagtgc
    12781 cgtcaactgg ggatttccta taaagcgtta gatatgttag aacctttaga ccatctctca
    12841 gaacgtgtaa caaatttttt gttagagaat cccgatatcg atgggatttt tacgatcaac
    12901 gatccaatgg cattagctgt cattgatatg cttgaaaaaa tagggaaaaa cgtaccagag
    12961 gatgtccaag ttatcggatt tgatggtcaa aagatgacag aaggagatcc ttatctagtc
    13021 tcaacgattg cccaacctac aaagcaaatg gcggaagtgg cagtggcaaa attaattgct
    13081 gtcattaatg gagagacagt agaaaaacgt accgtattgc cgattgaatt tggcgaaggt
    13141 gacacaacga aacctttagt gagaaacgat tttgcttagt gcaaatacgt ttcttttttt
    13201 gaaaatgagt gaatgaaagg tgtagataaa atgttatatg gtggaataga agccggtgga
    13261 acaaagtttg tctgtgggat tggtaatgaa caattacaag tgatagaacg gaaaagtttt
    13321 cctaccacaa caccagaaga aacgatggag caagtgtttg atttctttga taagtacaaa
    13381 ggaaatatcg catcaattgg tgtaggagca tttgggccta ttgatattca aatcgaatca
    13441 agaacttacg gatatatcac caacacaccg aagttagctt ggcaaaatta tcattttgta
    13501 ggagctttgg aaaacgcctt gtcggttccc atctattgga ctaccgatgt aaatgctgct
    13561 tgttatggag aatatatggc agggtatgga aaaggtaaaa atagtgttct gtattacacc
    13621 gttggtacag gtgttggtgg tggtgctgta gtggatggga aaatagtcgc aggttttagt
    13681 catcctgaaa tgggacatat gctcttacaa cgtcataatg aagatcaatt tgaaggaatc
    13741 tgtccttttc atcatgattg tttggaaggg ttagcatcag gacctgcgat tgaaaaacgc
    13801 tttggaagaa aagcccagac tatctcattt gatcatcctt attggacgat tgaggcagat
    13861 tatcttgctc agtgtgtgta taacacaacg ttactatatg caccggaagt aataatttta
    13921 ggcgggggtg taatgaaaca gccgcaacta ctcgagttga ttcgccaatc gtttaaagaa
    13981 aaaatgggtt cctacgtgga tacgcctact ctcgaagaat atctactaac tccaaggctt
    14041 gctgacaatg cgggcttgat tggctgccta gctttagcaa taaagaaact caatcattag
    14101 aatagtattt attagaacta ttcataacat accggaagtt taaaagggta ttctgaaact
    14161 taaatagtct caattcattc ttctttcttg agaatctgag tagagacttt tattctgttt
    14221 caaattaata taattacgaa atgatattga gtacaagaaa gttcttttta ccagatcttt
    14281 taaatcgaaa aatctgatat atatatttat agtactaaaa atctgattag ataaaaagct
    14341 aattcaatag gaaaataaag gtagtgaata aaagcaaagc ccttgttagt agttaatcta
    14401 acaagggctt tggtagggaa aatggtagga aaccatctta ttttttacga aaatcaggaa
    14461 ctttagtaat tgaataatgt tgatttatag gtattcctca tccatattat gttggctctt
    14521 tattcccact ccactgttgc aggtggtttg ctggtaatat catacacgat acggttaacg
    14581 tgtgcgactt catttacgat acggacactg attttttgca agacttccca agggatacgg
    14641 gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac ggatacctac agtgtagtca
    14701 tacgtacggc catctcccat aaccccaaca gagcgaatgc ctggtaggac ggtgaagtat
    14761 tgccagatgt cgcgatctaa accggcattg gcgatttctt cgcgcaagat agcgtctgat
    14821 tcacggacga tttctaattt ttcttctgtc aattcaccaa ggacacgaat acctaagcct
    14881 ggtccaggga atggttggcg ccaaacgatg ctgtcaggca tgcctaattg ggtacctaat
    14941 tcacgtactt cgtctttgaa caatgtgttc aatggctcga tcagcttgaa ttgcatgtct
    15001 tcaggtaatc cacctacatt gtgatgtgat ttgatggttt gggcagtttc agttcctgat
    15061 tcgatcacat cggtataaag ggttccttgt gcaaggaaag aaacgccttc ttcaccggca
    15121 agtttggttg cttcatcatc gaagacatat acaaattcat ttccaatgat tttacgtttt
    15181 tcttctggat cagacacgcc agcaagtttt gataagaaac gttctttggc atcgactttt
    15241 ataatgttca agccaaattt tccgccaaga ctttccataa cttgttctgc ttcaccttta
    15301 cgcaacaata cgtgatcaac gaagatacat gttaattgat cgccgatcgc tttttgtaaa
    15361 agaacaccaa caacagagga atctactccg cctgaaaggc ctaataaaac ttttttgtca
    15421 ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga agttatccat tgtccagtca
    15481 ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca agtcatttcc atattcggaa
    15541 tggcgaactt ctgcatggaa ttggattccg tacattttgc gttctgcatt ttggatcgat
    15601 gcgatggggc aatctttgct ggtagcaacg acatcgaaat cagtcggtgc ttgggtaact
    15661 aggtcaccat gactcatcca aacggtttgt ttttcaggag tgttttggaa aagggccgct
    15721 tctgcagaag tgatttccaa ttctgctttt ccgtattcac ggtttttggc tggtttaact
    15781 tttccgccta atttataagt aatcaactgc ataccgtaac agattcctaa aactgggatc
    15841 cccaaatcaa aaatcttttc atcaatacca aaggccttct catcataaac actgtttggt
    15901 ccgccagaaa ggatgattcc tttagggttc attgccttta cttcttctgc ggtaatgcgg
    15961 tggctcatca actctgagaa aacaccgaac tcacgaatac ggcgagtgat cagttggtta
    16021 tactgactac cgtaatcaag tacgataatt tt
//
LOCUS       DAYYAR010000057        15913 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000057 DAYYAR010000000
VERSION     DAYYAR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.057"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACHAT0_001573"
     CDS             226..894
                     /locus_tag="ACHAT0_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8451517.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACHAT0_001574"
     CDS             complement(1094..1414)
                     /locus_tag="ACHAT0_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451518.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACHAT0_001575"
     CDS             complement(1451..2833)
                     /locus_tag="ACHAT0_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8451519.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACHAT0_001576"
     CDS             complement(2989..4176)
                     /locus_tag="ACHAT0_001576"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8451520.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACHAT0_001577"
     CDS             complement(4183..5214)
                     /locus_tag="ACHAT0_001577"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8451521.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="ACHAT0_001578"
     CDS             complement(5260..6684)
                     /locus_tag="ACHAT0_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8451522.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="ACHAT0_001579"
     CDS             6859..7764
                     /locus_tag="ACHAT0_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8451523.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACHAT0_001580"
     CDS             7855..8532
                     /locus_tag="ACHAT0_001580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8451524.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACHAT0_001581"
     CDS             8529..8699
                     /locus_tag="ACHAT0_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451525.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACHAT0_001582"
     CDS             8813..9709
                     /locus_tag="ACHAT0_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8451526.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACHAT0_001583"
     CDS             complement(9764..11134)
                     /locus_tag="ACHAT0_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8451527.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACHAT0_001584"
     CDS             11372..12118
                     /locus_tag="ACHAT0_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8451528.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACHAT0_001585"
     CDS             12115..12954
                     /locus_tag="ACHAT0_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8451529.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACHAT0_001586"
     CDS             12955..13905
                     /locus_tag="ACHAT0_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8451530.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACHAT0_001587"
     CDS             14046..14798
                     /locus_tag="ACHAT0_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451531.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..>15913
                     /gene="queG"
                     /locus_tag="ACHAT0_001588"
     CDS             14817..>15913
                     /gene="queG"
                     /locus_tag="ACHAT0_001588"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8451532.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEV"
BASE COUNT         5156 a         2987 c         3209 g         4561 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agt
//
LOCUS       DAYYAR010000058        15853 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000058 DAYYAR010000000
VERSION     DAYYAR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.058"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..833
                     /locus_tag="ACHAT0_001589"
     CDS             81..833
                     /locus_tag="ACHAT0_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8451533.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            830..1744
                     /gene="pfkB"
                     /locus_tag="ACHAT0_001590"
     CDS             830..1744
                     /gene="pfkB"
                     /locus_tag="ACHAT0_001590"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8451534.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1762..3693
                     /locus_tag="ACHAT0_001591"
     CDS             1762..3693
                     /locus_tag="ACHAT0_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8451535.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3804..5363)
                     /locus_tag="ACHAT0_001592"
     CDS             complement(3804..5363)
                     /locus_tag="ACHAT0_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8451536.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5518..7752
                     /gene="pcrA"
                     /locus_tag="ACHAT0_001593"
     CDS             5518..7752
                     /gene="pcrA"
                     /locus_tag="ACHAT0_001593"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8451537.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7769..9805
                     /gene="ligA"
                     /locus_tag="ACHAT0_001594"
     CDS             7769..9805
                     /gene="ligA"
                     /locus_tag="ACHAT0_001594"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8451538.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9911..10216
                     /gene="gatC"
                     /locus_tag="ACHAT0_001595"
     CDS             9911..10216
                     /gene="gatC"
                     /locus_tag="ACHAT0_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8451539.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10216..11688
                     /gene="gatA"
                     /locus_tag="ACHAT0_001596"
     CDS             10216..11688
                     /gene="gatA"
                     /locus_tag="ACHAT0_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8451540.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11685..13115
                     /gene="gatB"
                     /locus_tag="ACHAT0_001597"
     CDS             11685..13115
                     /gene="gatB"
                     /locus_tag="ACHAT0_001597"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8451541.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13125..14180
                     /locus_tag="ACHAT0_001598"
     CDS             13125..14180
                     /locus_tag="ACHAT0_001598"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8451542.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14291..15664
                     /gene="rlmD"
                     /locus_tag="ACHAT0_001599"
     CDS             14291..15664
                     /gene="rlmD"
                     /locus_tag="ACHAT0_001599"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8451543.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15667..15795
                     /locus_tag="ACHAT0_001600"
     CDS             15667..15795
                     /locus_tag="ACHAT0_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8451544.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5391 a         2901 c         3478 g         4083 t
ORIGIN      
        1 tttttatgaa cgtttttgac tgatttatat tgcgagtaga aaaaaaatat gctattattt
       61 gtattgaggt gatcgtgaaa atgcttacag aggaacgtag acaaaaaatc ctagacttgc
      121 ttgaacagca gaaaatcgtg aaatcacaag atcttgtaaa cctttttaat gcttctgaat
      181 caacgataag acgtgattta caagagctgg agaatagtgg gtttctagaa agagttcatg
      241 gcggagctaa gaaagagcaa ttattgaatt ttgagcagaa tatgagtgaa aaatcactca
      301 aaaacgttca cgaaaaacag caaatcgcca aatcggcagc tgaaaccatt aatgacgaag
      361 atgtcattta tttagacgct ggctctacta cgttagaaat gattcctttt ctgcaaggaa
      421 aacagatcac agtagtgaca aattccgttc aacatgcggc aaatttagtt gatttggagc
      481 ttgctaccat tattttaggt ggaacgataa agctggcaac taatgctgtt ttaggatcta
      541 atgcgataga acaactaaaa aattttcatt ttaataaagc atttatggga atgaacggtg
      601 cccatcttga acaaggattc acgactccgg acccagaaga ggcagctgtt aaacgtttag
      661 ccatgaaaaa ttccgaagag acatttgtat tgattgatca taccaaattc aaccagttgt
      721 ccttcacctc tgttgcacct ttggaagcag ccacgatcat aaccgagcgt tgtccattag
      781 aattcgttaa tgaattccgt gaaaagacaa caattaagga ggcaatacaa tgatttacac
      841 agtaacactt aatccatcaa ttgattatat cgtacgcgta gatgggttaa aactaggcga
      901 tttgaaccga atgaaggaag acttcaagtt acctggagga aaagggatca atgtttcccg
      961 tattttgaaa cggatcgatt ctcaatctac agcactaggg tttttaggag ggttcactgg
     1021 cgaatttatt tcgaagtggc ttgaaaaaga acaaatccag tctttgttca caaccgtaaa
     1081 agaagataca agaatcaata tcaaactaaa atcagatact gaaactgaaa ttaacggttt
     1141 gggcccatct atttcagatg aagaaatcga tgagttgaag caagcattag gaacagtagg
     1201 acaaggagat atcgtcgtat tgtcaggaag cgcgccggct agcctgcgaa aaggatttta
     1261 tcaagaactg attgatatca ttcgagaaaa aggtgcagat ttcgtgattg atacaacagg
     1321 tgatgaccta aaaaatgcac ttgttaagaa accattgttg atcaaaccaa acaatcacga
     1381 attagctgag ctttacaata caaccttcca atctgtagaa gaaatcattc cgtatggtaa
     1441 aaaattgcta gctgaagggg cacagaatgt gctgatctca atggctggag acggtgcttt
     1501 gctcttcacg gaacaaggaa cttataaaac gaatgtatta gttcgcccat tgaaaaattc
     1561 agtaggtgca ggggattcaa tgattgccgg ctttattggg gcttacagcc gtacgaaaga
     1621 tccaatcgaa gcctttaaat ggggagttgc ttgcggaagc gcaacagctt tttctgatga
     1681 cttggcagct cgcgaattga ttgatgagtt gctgccagaa gttgaaatca caaaaattga
     1741 ttagtttcaa ggaggataat catgaatatc aaggatttaa tggtcaaaga agcgatgatc
     1801 atggatttgc aagcgactga taaaaaaggc gcaatcgatg aaatggtcca aaaaatgttt
     1861 gatgccggcc ggatttccga tatcgaaaca tataaagaag gaattctagc ccgcgaagca
     1921 caaacttcta caggtcttgg tgatggaatc gccatgcctc acgcgaaaaa cagtgcagtt
     1981 aaagaagcaa cagttttatt cgcaaaaagc caaaaaggag tagattatga agccttagac
     2041 ggacagccaa cttatctgtt tttcatgatc gcagctccag aaggcgcaaa cgacacacat
     2101 ttgcaagcat tagcagcact atctcgattg ctgattgatg cagatttcgt aggaaaatta
     2161 aaagaagcaa atacaccaga agaagtccaa gacttgttcc aaacagctga agagcaaaaa
     2221 gaagcagaag aaaaagcaga acaagaaaca caagctgcag aagcttctgc tcatccagaa
     2281 agaaaattcg ttgttgccgt tacagcttgt ccaacaggga tcgctcacac ttacatggca
     2341 gaagatgctc tgaagaaaaa agcgaaagaa atgggtgtgg agatcaaagt cgaaacgaac
     2401 ggttcagaag gaatcaaaaa ccgattgact gctgaagaca tcgcccgtgc agaaggtgta
     2461 atcgttgcag cagacaaaaa agtagagatg aatcgctttg acggaaaaga actgatcaat
     2521 cgtcctgtca gtgatggtat ccgtaaacca gaagagctga tcaatctagc ccttagtggt
     2581 actgcaccag tattccatgg tgatggaaaa gaaagcagtt cagaagaagg aaatgcggat
     2641 ggtactatcg gacaaagaat ttacaaagat ttgatgaacg gcgtatcaca catgttacct
     2701 ttcgttattg gtggaggaat cgctattgcg ctttctttca tgatcgacca atttattggc
     2761 gtgccacatg atcaattatc aaatctaggg aattacaatc aagctgctag ctggttcaac
     2821 caaatcggac aagctgcatt tggtttcatg ctgccggtat tagcaggatt tattgcttca
     2881 agtatcggtg accgccctgg tttgatcgtt ggatttgctg ctggtgcatt agctaataca
     2941 ggcggtgcag gattcttagg tgcactgatt ggtggattct tagctggtta tgtcatcgta
     3001 tttttacgta aattattcaa gaacttacca aaatcattgg atggaattaa aacgatcctc
     3061 ttttatccag tgtttggctt actgattact ggtttcttga tgttgctggt caatgtccct
     3121 atgaaagcaa tcaatgatgc attgaacggt ttcttgacaa gtatgagtgg ttcgaatgct
     3181 gctttacttg gtgcattatt agctggaatg atggcggcag atttaggcgg tccaatcaac
     3241 aaagccgctt atgtgtttgg tacagcaact ttagctacga ctgttgcaac tggcggaagt
     3301 gtcgtgatgg cttctgttat ggctggagga atggtacctc cattggcaat tttcgttgct
     3361 acacgtttgt ttaagaataa attcagcaaa acagatcaag atgctggttt gacgaacatc
     3421 gtgatgggac tatcgtttgt cacggaagga tcgattccat ttgcagcagc tgacccgatt
     3481 cgtgccattc caagttttat tattggttct gctttaactg gcggattagt cggagctttc
     3541 gggatcaaat tactggctcc tcatggcggg attttcgttg tattcttact gagtcacccg
     3601 gtcatgtatt tggtgtttat tgctatcgga gcagtcgtat ctggtgtgat ttatggttta
     3661 ttgagaaaag ctcctgcagc agcagaagca tagaaataaa aaagaagagg ctggtttttc
     3721 ccagtctctt ctttttttat agaatcatat agaatcaagt agtgtagatg aatagttgtt
     3781 gtaaaatagt ttttccatgt tcgttaaaat gattgcttca aatttgtgat agaagattta
     3841 gcctcgattt cctgcttgat tcttggcaag tttgcttcat cagtcaagat aaccaaaata
     3901 tctcctacat tcatgatcgt atcaccgtga gtcaagattt cctgatttcc tctgcgtata
     3961 gagattagca gcatattttt aggccaattg aagtctcgta ccattaatcc gtctaaagaa
     4021 ctctctgccg ttactggaaa atcaaaagtt gtccttgcac cttttagtga ggcggtctga
     4081 gtatttccaa gtagacgttc caataatgat tcgtaaattg gattaccgcc gagaaggtca
     4141 ttgacgatat aagcaacaag agcagccaat cctaaaggca tcagatgatt aagagagcca
     4201 accatttctg tcactaaaat aattgctgtc agcggtgctt ttccaatcgc tgtgaaataa
     4261 ccagccatgg caataacgac aaaatccttg atgtagattg gatccatccc gaaatcaacc
     4321 ataatgtttc cataaaagct accgattaaa gcccccaatg tcaatatcgg aaggaaaatc
     4381 ccccctggaa gaccaccacc gtaagaaatc atggaaaaaa cgaatcgaag aacaaacaaa
     4441 ccgataatca taatgattgg caaggaagca tgtccaaaag ctaaaacgat ttgattgcct
     4501 ccgcctagag tactaggcca taaataacca attggcaaaa tcagcaagaa aggaacgaac
     4561 ccgtgaaaat aagctggtaa aggcaatcgg ttgtaaaatt ttggcaaagc cagcaagacc
     4621 ttctgataaa taaaacctaa gatgcctaga agtactccta agatcactaa ggaaccataa
     4681 tatttcaacg gcaaagaagg aaggtcacct agatataaaa caggtctcaa gccgaaaaag
     4741 taaagagaga cgaagttagc agtgattgct gaagcaaatg atgtcagcca aacaagtgga
     4801 gaaaaggagt gatggatttc ttctaaaaca aagagtaatc ctgcgatagg agcattgaat
     4861 gctgctgcaa gtccgctact tgcaccgcta gaaattaaaa tcttttcttc tgaagatgga
     4921 ctccgaaaca attgtccagc tccttgtcca atggatgcgc caagctgtat agaaggtcct
     4981 tctctaccta gaaaaaggcc cgcaccaact gaaagtacac cgccaatgaa ctttttccat
     5041 aaaacagaga accagttcat tgataattcc ccgcgaagct gtccctctac ttgtggaatc
     5101 ccgcttccct tgatatccgg ttcatttttg accagataac ccacgaaaaa tgctgcaagg
     5161 atgcagcaaa tagctaggcc aattaagtaa atcggctgtt cccgactcat ttcatagata
     5221 cttcgaacta aatcgcttag cttttcgata gataatcgaa acaggctaac gacgattcct
     5281 gtcagcagtc caacaaatat tccttttaga ataaaatata tctgagtctt atctaatttc
     5341 ttgatttttc cttttttttc cattcaaacg cctcgctttc ttttaattta gcgcgaaatc
     5401 aacgaattga caaggaaaaa tccataaaaa gaaacaaatg attttgaact agctagttta
     5461 atgaaagcca agaaaatgct ataattaaca agattgataa aagaaaggat gttgttaatg
     5521 tcatccaaag ctgaattatt aaacgggatg aacccccgtc agaaagaagc tgttcttcac
     5581 actgacggac cattactgct aatggcaggt gcaggaagtg gaaagacgag agtgctgaca
     5641 caccgcattg cctatttgat tgaggaaaaa gaagtcaatc cttggaacat cctagcgatt
     5701 acttttacca ataaggctgc aaaagaaatg aaagaacgtg tcaatgcgat tctcgcatct
     5761 ggaggcgaag atgtctgggt atcgactttt cactcgatgt gtgtgcgtat tttaagaaga
     5821 gatgttgatt ttatcgggta taaccggaac tttacaatca tcgattcttc tgaacagctg
     5881 accttgatga aacggatact aaaagaactg aacattgatc cgaaaaaata tgatcctcga
     5941 agtatattag gaacaatctc tcaagcaaaa aacagcctgc agacccctca ggattttgca
     6001 aaaatgcaag gcagttatta cgaagagatt gcggcaaaat gttatgctgc ttaccaaaaa
     6061 gagcttcaat ataatcaatg tatggacttt gacgacctga tcatgaatac gattcgatta
     6121 tttgaagaac atcctgattc actcacatat tatcaaaaca aattccatta tattcatgta
     6181 gatgagtacc aagatacaaa tcacgcacag tatacgctag tcaatttatt ggcaggtaga
     6241 tttaggaacc tgtgcgtcgt gggagatgca gatcaaagta tttacggctg gcgcggtgca
     6301 gatatgcaga acatcttgga ttttgaaaaa gattatccag atgctgcagt aattttgcta
     6361 gaacaaaact atcgctccac gaaaaacatc cttagcgcag caaaccaagt cattgaaaac
     6421 aatagcaatc gaaaaccaaa aaacttgtgg acagaaaaca aagaaggaaa caagatcact
     6481 tactaccgtg cagataatga acgagacgaa acccgtttta tcgtagatcg gatgcaagaa
     6541 gagattcgaa gcaatcatcg gaattacggc gactttgcca tcttgtaccg gacgaacgca
     6601 caatctcgtg taatggaaga aactctgttg aaagcaaata ttccttataa aatggttgga
     6661 gggcacaagt tctacgatcg taaagaaatc aaagatattt tagcgtattt gaatgtgctg
     6721 gcaaatcctc aagattcaat cagttttgag cgtatcgtta attcgcccaa acgaggcatc
     6781 ggaccaggat ctatcgaaaa actacgcagt tttgcttctc ttcatgaatg gccactgctt
     6841 gaagcagcgc aaaatgtgga tctggcaaat atcggtggaa aagcaggaca gcaattaggt
     6901 gcatttggag agatgatcca agaagtcact cagatgatcc catacttgac agtgacagaa
     6961 ttgacgaaag aggttttgga tcgtagcgga tatctagaag acttaaaaat ccaaaataca
     7021 ttagaagcac aagcacgcat cgaaaacttg gaggaatttt taacggtcac acaagaattc
     7081 gacaaacaat tcgaacagca aaatgaagaa gacgctgatg caccagaaga aaaactaacg
     7141 gtcttcttaa atgatctggc acttgtttcc gatattgaca atttagaaga agatgcttcc
     7201 caagtgacat tgatgacttt gcatgcagcc aaaggattag aattcccagt tgtattcctg
     7261 attggtctag aagagggggt ttttccttta tctcgagcat tgatggaaga aagcgaactg
     7321 gaagaagaac gccgtctcgc atatgtgggg atcacgcgag cagaagaagc gttgtattta
     7381 acaaatgctt tctccagaac attatatgga cggacgcaat ataaccgacc ttctcgtttt
     7441 gtagaagaaa tcgatcagga gttactggaa atcgaaggca tgcgtcctac gcctaaaaaa
     7501 acacctgtat tcgctaaaaa aacagcatat agctataaac agccagagac agcggttgtt
     7561 ccctctaaaa gtgctactgg aggggaaaat aacagctgga aacctggaga taaagtcaaa
     7621 cacaaaaaat ggggtctagg aacagtcgtc cgagtaagcg ggacgtcgaa ggatctagaa
     7681 ctagatgtgg ctttcccaag tcaaggagta aaacgtcttt tggctgcctt tgcgccaatt
     7741 gaaaaagctt agtagtggag ggtaaatgat ggaacatgaa atgacgattg acgaagctgc
     7801 taaacgagca gaagaacttc gtacaagact gaaccaatgg tctcgcgaat actacgtaga
     7861 agataaacca acagtggaag actatgttta tgataaagaa tacgcagaat tagtagcaat
     7921 tgaggaacaa tatcctgatt tgatcacgtc tgattctccc actcaacgag taggcggaaa
     7981 agtattagaa ggttttgaaa aagtcactca tgatattccg ctttacagtt tgaacgacgt
     8041 tttcagcaaa gaagagctga tagcttttga tcaacgtgta caaaaagcag tgggacgtgt
     8101 agttgactat tgctgtgaac taaaaataga tggactttcc gtttctttgc gttatgaaga
     8161 tggaaatttc gtgagaggtg cgactcgcgg ggatggaact gtaggagaaa acatcactga
     8221 aaacctaaaa actgtccggt cggtcccaat caaattaaaa gaaccaatga acatcgaagt
     8281 aagaggcgaa tgtttcatgc caaaacggtc atttgtccaa ttgaaccaag acagagaagc
     8341 agagggaaaa gatatctttg cgaatcctcg gaacgccgca gctggttctt tgcgtcagtt
     8401 agattctaaa atcacagcga aaagaaatct ggacactttt ttatacacag tagctgattt
     8461 cggtcctatg caagcaaaaa cccagtatga tgcgctagaa gagctagaaa aaattggatt
     8521 ccacacgaat cgggaaaaac gtctatgtca ttcgattgat gaagtgtggt catacattga
     8581 agaatatcat gacaaacgtg tggatttgcc ctatgaaatc gatggaatcg tcatcaaagt
     8641 gaatgaattc tcgctacaag atcaacttgg ttttactgtc aaagcaccga gatgggcagc
     8701 agcttacaag tttccgccag aagaagttga aacgctaatc gaaaacatcg aatggacagt
     8761 cggtcgaaca ggggtagtaa cgccaacagc tatcatgaca ccagtccgag tagcaggaac
     8821 gacagtaagc cgtgccagcc tgcataacgg tgattatatt aagttgaagg atatccgctt
     8881 gaaagataca gttctgattt ataaagcggg agacatcatc ccagaagttt cgcaagtcgt
     8941 gttagataaa cgacctaaag acagcgaaga ataccaattg cctacacatt gtccagtttg
     9001 tggaagtgaa cttgtccatt tggatgaaga agtagcctta cggtgcatca atcctaaatg
     9061 ccctgctcaa atgaaagaag gtttgaacca ctttgtttca agaaatgcga tgaatatcga
     9121 tgggttgggg ccgcgcgtac tagaacagat gtatgataaa aaactagtag cagatgttgc
     9181 tgatctttac aaattgacag aagaagagtt attgacgttg gacaaaataa aagaaaaatc
     9241 agcaaataat attttgacag caatcgacaa cagcaaagat aattcggtcg agcggttgat
     9301 tttcgggtta ggaatccgac atgtcggagc gaaagcagca aaaattttag cagaacattt
     9361 cggtgatcta gaaacattga gtaaaagtga ctatgagtcg attattgcgc ttgatacgat
     9421 cggtgatatc attgccgaca gtgtagtcac ttatttcagt aacgaagaag tccatgaatt
     9481 gatgaatgaa ttaaaacaag caggcgtcaa tttcgaatac aaagggcttc gaaatgccca
     9541 attgcaagaa gtcgagtctc cttttaaaga aaaaacagta gtccttactg gtaaactaac
     9601 acgatttact cgtgaagaag caaaagagac gatcgaaaat cttggcggga aagtgactgg
     9661 tagcgtatcg aaaaaaacgg atatcgtggt agcaggagaa gatgctggca gcaagctgac
     9721 caaagcacaa gaattaggaa tcgaagtttg gacagaagat caaatggcag atgcattggc
     9781 taaaagtcgc tcagtagaag aatgaaaaat gagttttttc agacaaattt caataaacaa
     9841 tttcagacac ggcactcttg tgttaaaata gagagcaaag tttttaactc gtttagaaag
     9901 aaggggtagt atggcgatta gtgaagaaca agtgaaacac gttgccaaat tagcaaaact
     9961 tgcgttcgca gatgaagaac ttgcaagttt taccgaccaa ttaggtaaaa tcatcgatat
    10021 ggttgaaatg ctggaagaag tagatacaga aggtgttcca tttacgtcga atgtggttga
    10081 aacagtaaac gtgatgcgac aagatcgggc gcaagctgga tggaaccgcg atgaattatt
    10141 gaaaaatgtg ccggaaacag aagacggctt tatcaaagta cccgcaatca tcgataatgg
    10201 ggaggctggc gcataatgac agaattacac aatcgttctc tagaagaact gcatcatatg
    10261 cttgtatcga aagaaatcac tgtacaagat ttgacaaaag caacatttga acgaatcaaa
    10321 gaaacagagc ctgaggtcga agcattcatt acattgaacg aagaaaaagc attggaacag
    10381 gcaaaagctt tagatgaaaa agggattgat gaaagcaacg tccttgctgg tattccaatt
    10441 ggtatcaagg ataatatcgt aacaaaagat atcttgacaa ccgctgcatc taagatcctt
    10501 tacaattttg aaccaattta tgatgcaacg gtcatggata aaatctatca agcagatatg
    10561 atccctgttg gtaagctaaa catggatgaa tttgctatgg gtggaagtac agaaacttct
    10621 tatttcaaga aaacaaaaaa tgcttgggac ccaacaaaag tacctggtgg ctcttctggt
    10681 ggttctgcag cagcagttgc agctggacaa attccagttt ctttaggtag tgatactggg
    10741 ggaagtatcc gccaaccagc agcttttaac ggcatcgtag ggatgaaacc tacttatggc
    10801 cgtgtctcac gttttggttt gattgccttt gcttcatctc ttgaccaaat cggaccaatg
    10861 acccgtacgg tgaaagataa tgcattagca ttgacagcta tcagcggtta tgacgaaaaa
    10921 gacggtactt cttcaggcgt ttcagttcct gattttgcag agggactgac aggcgacatc
    10981 aaaggaatga agattgcttt acctaaagaa tacttaggcg aaggtgtggc ccctggcgta
    11041 aaagaagctg tattgaaagc agcagaaaca ttccgttcat taggtgctgt tgtggaagaa
    11101 gtcagcttgc ctcattcaaa atacggtgtg gctgtttatt atatcattgc ctcatcagaa
    11161 gcaagttcca acttgcaacg attcgatggt atccgctacg gttatcgctc agaaaatgtg
    11221 aaatcattag atgatgtata tgtcaactca cgttcagaag gattcggtga agaagtaaaa
    11281 cgccgtatca tgttaggtac attctcctta agtgctggtt attatgacgc ttacttcaaa
    11341 aaagcgggac aagtccgtac attgatcaaa cgagattttg aaaaagtctt tgcagattat
    11401 gacttgatca tcgggccatc ttcaccaaca gttgcattcg gaatcggtga gaacattaac
    11461 gatccaatca caatgtacat gagtgacatc ttaacgatcc ctgtcaactt ggctggactt
    11521 ccaggtatgt ctgtaccggc aggtttatca gaaggattac cagttggatt gcagatcatc
    11581 ggtaattatt ttgatgaaaa aacgatgtat caagcggcat atgcgtttga acaagcaaca
    11641 gaattccata aacaacaacc agctatttta ggagggaaag acgaatgaat tttgaaacag
    11701 tcattggact agaagtccac gtcgagttaa aaaccaactc aaaaattttc tctccagcgc
    11761 cagctcattt tggtgcagaa ccaaacagca atacaaacgt gatcgactgg ggttatccag
    11821 gcgtgctgcc agtaatgaat aaagctgcgc tggaattcgg gatgaaagca gctttggcat
    11881 taaactgtga aatctctaaa gatactcatt ttgaccggaa aaattacttt tacccagata
    11941 atccaaaagc atatcaaatt tcccaatttg atcaaccaat cggtcatgac ggatggatcg
    12001 aaatcgaagt agaaggtaaa aagaaaaaaa tccggatcga acgtgtccat ttagaagaag
    12061 atgctggaaa aaacatgcat ggtattggcg gttattctta cgttgatttg aaccgacaag
    12121 gcacgccttt gatcgaaatc gtttctgaag ctgatatgcg ttcaccagaa gaagcatacg
    12181 cttatctaga agcattgcga tcaattatcc aatttacaga agtcagcgat gtgaaaatgg
    12241 aagaaggctc tatgcgctgt gatgccaata tttctcttcg cccttatgga caagaagagt
    12301 tcggtacgaa agcagaattg aaaaacttga actctttatc tttcgtgaaa aaaggtttag
    12361 cttttgaaga aaaacgtcaa gcaaaagttc ttctttcagg aggagaaatc caacaagaaa
    12421 cacgccgttt tgacgaaaca acgaataaaa cagttttgat gcgtgtcaaa gaagggtcta
    12481 gtgattatcg ttacttccca gagcctgata tcccgaaatt cgtgatcgat gacgaatgga
    12541 ttcaaaaagt aaaagctagt ttgcctgaga tgccagcttc tcgtcgcgaa cgctatatcc
    12601 gtgagtttgg acttccagag tatgacgcaa tggtattgac acttacaaaa gaaatgtctg
    12661 atttttttga agctgttttg gaaaatggtg cagatgccaa gcaagcatct aactggttga
    12721 tgggtgaagt ttctgcttac ttgaacagtg agaaactgga attagctgag acaaaattga
    12781 cacctgagaa cttagcagga atgattcgtt tgatcgccga tggaacgatc agctcaaaaa
    12841 tcgccaaaaa agtcttccgt gaattgatca caaatggcgg agaagcccaa gaagtagttg
    12901 aaaaaaatgg ttgggtgcaa ttatctgatc cgaacaaact actgccaatc attaatgaaa
    12961 ttttggataa caaccaacaa tctgtcgatg atttcaaaaa tggaaaagat cgtgcggttg
    13021 gcttcctagt cggtcaaatc atgaaagcaa ctaaaggaca agcaaatcct ggtgtcgtga
    13081 ataagctact taaagatgaa ttagcgaaac gatagaggct gaaaatgaaa aaagcacgcg
    13141 taatttataa ccccacctcg ggaaaagaat tattgaaaaa aaatttggcg gatatcctac
    13201 aggcattaga agaagcagga tttgaagcca gcgcctatgc aactacacca gaaccagatt
    13261 cagccaagaa tgaagcatgt agagtcgcag aactaggttt tgatctagta gtggcagctg
    13321 gtggcgatgg cacaatcaac gaagtggtta atggaatcgc tccgcttgaa caccgtccta
    13381 aaatggccat catcccagct ggtacaacga acgactatgc tcgagcatta aaaattccta
    13441 gagataatat taaagcagca gcagaagtga taaaaaaaca ccaaaccgta aaaatggaca
    13501 tcggcctttc tgataacagt tacttcatca atatcgctgc aggtggctat ttgacagaat
    13561 tgacttatga agttccatca gaactaaaga gtatttttgg ttatcttgct tatcttgcga
    13621 aaggagcaga gatgctccct cgagtaaagc caatcaaaat gcgcttgaag tatgatgaag
    13681 gggaatatat cggaaatgct tcgatgtttt tcctagggct gacgaactct gttggaggat
    13741 tcgaacagat cgcgccagat gccaaattgg atgatgggaa gttttcttta atcattgtga
    13801 agacagcaaa tatctttgag atcttgcata ttgcagcact catgctgaat ggcggtcgac
    13861 atgtcgatga tccaagaata atctatacca aaacaagcag tttgttcgtt gaaacagaag
    13921 atgatatcaa tcgaccagta atgatcaact tagatggtga atacggcggg gaagcaccga
    13981 tgcatttcca aaatcttcat caacacattg agttctttgc caataccgat cagattcctg
    14041 atgaagcaat cacaggtaca gatgaagaag aattagaggt tgtcagcaaa gaattcgtaa
    14101 aagaagtcga acgtttaacg gatgaagata tcgacggtga cggaaaaata gcagacgaaa
    14161 cacctaatca aaagaattaa tcaaaagccg gtgctgaacg tagtaaggtc agtgccggtt
    14221 ttagttgtgt aattagctga tttatagtag agttcagtag gattgaaaaa taggcttaaa
    14281 ggagcaaaat atgacagaac agcaaatagt aaagaaaaac gaacgatata cagtcgaaat
    14341 cgtcgacttg agttatgaag gaatgggggt agcaaaaatc gatggttatc cattatttat
    14401 cgaaaatgca ctccctggag aagaagtaga aatactagta gtaaaagcag gcaataagtt
    14461 tggatatgga aaagtcgaaa catttattac acaatcacct gatcgtcagc ctgtagaaaa
    14521 caatgcatta cttcgcatag gtatcgcacc tttagcccat ttgaagtatg aacaacagct
    14581 tgtcttcaaa caaaaacaag tagagcatgt aatggcgaag attgctaaga tgccagaagt
    14641 tcctgtactt ccaacggtcg gaatggcaga accattcggc taccgtaata aagcacagat
    14701 ccctgttcgc cgtatcgatg gacagttagc caccggtttt tacaaaaaga atagccatga
    14761 tctagtccct atcgaagatt tttatatcca agatcctgtc atcgatcaag caatcaaagt
    14821 agtcagagat atcttgcaac ggttccaagt acgaggatat aatgaagaaa aaaatgaagg
    14881 acagcttcgt cacatcatta tccgtcgtgg tcattattcc catgaaatga tggtcgtttt
    14941 agttactcgt aaagaaaaat tctttaaatc caaggaaatt gctgaagcta ttcatgaaga
    15001 attgccagaa gtggtttcgg tcatccaaaa tatcaatgaa gaaaaaacaa atgtgatttt
    15061 aggagacaaa gaaaaagttt tatatggcag agactatatc gaagatcagc tattaggaaa
    15121 aacctatcga atctcagcaa agtcatttta tcaagtaaat actcaacaag cagagtatct
    15181 gtaccaaaca gccatcgagt ttgccgagtt agataaaaaa gacattgtga tcgacgctta
    15241 ttcaggaata ggtacgattg gcttgtcttt agcagacaga gtggataagg tgtatgggat
    15301 ggaagttatt ccagaagcaa ttgaagatgc acagttcaat gctttaaata ataaaatcga
    15361 aaatgttcat tatgaagtcg gaaaagcaga aaaagttatg aaaaaatggc aagaaaaagg
    15421 gatcaaacca tctgtgatcg tagttgaccc tccacgaaaa ggattagatc atcattttat
    15481 tgaatcagca atcgaaatgt caccagaacg aatcgtctat atctcatgta atccggcaac
    15541 ttttgcaaga gatgctaagt tatttgcaga agcaggttat gccgtgaaga aagttcagcc
    15601 tgtagatctt ttcccgcaaa cccatcatat cgaattagtc gcttcattcg ttaaaggaga
    15661 ataaaaatgg accaaaaaga aaaaatgaaa gaattgatca aaaagaaaca aggtggaggc
    15721 cgatccaaac aaatggaaac acctaagaat gatcgtaaaa acatgagaaa aggccctaaa
    15781 atctttaata aatagacgtg ctaaagaaag actaagaagt gttaactttt gttattgttt
    15841 cttagtcttt tta
//
LOCUS       DAYYAR010000059        15812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000059 DAYYAR010000000
VERSION     DAYYAR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.059"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..751
                     /locus_tag="ACHAT0_001601"
     CDS             68..751
                     /locus_tag="ACHAT0_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8451545.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="ACHAT0_001602"
     CDS             753..1589
                     /locus_tag="ACHAT0_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8451546.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="ACHAT0_001603"
     CDS             1600..3354
                     /locus_tag="ACHAT0_001603"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8451547.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="ACHAT0_001604"
     CDS             complement(3630..3971)
                     /locus_tag="ACHAT0_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8451548.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="ACHAT0_001605"
     CDS             complement(4039..6210)
                     /locus_tag="ACHAT0_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8451549.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="ACHAT0_001606"
     CDS             6421..7278
                     /locus_tag="ACHAT0_001606"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8451550.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="ACHAT0_001607"
     CDS             complement(7349..8206)
                     /locus_tag="ACHAT0_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8451551.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="ACHAT0_001608"
     CDS             complement(8191..10893)
                     /locus_tag="ACHAT0_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8451552.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="ACHAT0_001609"
     CDS             complement(10906..12195)
                     /locus_tag="ACHAT0_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8451553.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="ACHAT0_001610"
     CDS             12345..13391
                     /locus_tag="ACHAT0_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8451554.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="ACHAT0_001611"
     CDS             13393..15546
                     /locus_tag="ACHAT0_001611"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8451555.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4491 a         3303 c         2919 g         5099 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgag tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccatttag tggagaggtc gtgaaaaagc tg
//
LOCUS       DAYYAR010000060        15487 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000060 DAYYAR010000000
VERSION     DAYYAR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.060"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..512)
                     /locus_tag="ACHAT0_001612"
     CDS             complement(42..512)
                     /locus_tag="ACHAT0_001612"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8451556.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(671..1129)
                     /locus_tag="ACHAT0_001613"
     CDS             complement(671..1129)
                     /locus_tag="ACHAT0_001613"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8451557.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1300..2445)
                     /locus_tag="ACHAT0_001614"
     CDS             complement(1300..2445)
                     /locus_tag="ACHAT0_001614"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8451558.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2458..3375)
                     /locus_tag="ACHAT0_001615"
     CDS             complement(2458..3375)
                     /locus_tag="ACHAT0_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8451559.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3803..4366
                     /gene="efp"
                     /locus_tag="ACHAT0_001616"
     CDS             3803..4366
                     /gene="efp"
                     /locus_tag="ACHAT0_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8451560.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4467..4637
                     /locus_tag="ACHAT0_001617"
     CDS             4467..4637
                     /locus_tag="ACHAT0_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451561.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4852..6111
                     /gene="lysA"
                     /locus_tag="ACHAT0_001618"
     CDS             4852..6111
                     /gene="lysA"
                     /locus_tag="ACHAT0_001618"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8451562.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6098..6898
                     /gene="dapF"
                     /locus_tag="ACHAT0_001619"
     CDS             6098..6898
                     /gene="dapF"
                     /locus_tag="ACHAT0_001619"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8451563.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6901..7782
                     /gene="dapA"
                     /locus_tag="ACHAT0_001620"
     CDS             6901..7782
                     /gene="dapA"
                     /locus_tag="ACHAT0_001620"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8451564.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7779..8495
                     /gene="dapB"
                     /locus_tag="ACHAT0_001621"
     CDS             7779..8495
                     /gene="dapB"
                     /locus_tag="ACHAT0_001621"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8451565.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8501..9196
                     /gene="dapD"
                     /locus_tag="ACHAT0_001622"
     CDS             8501..9196
                     /gene="dapD"
                     /locus_tag="ACHAT0_001622"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8451566.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9212..10213
                     /locus_tag="ACHAT0_001623"
     CDS             9212..10213
                     /locus_tag="ACHAT0_001623"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8451567.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10210..11571
                     /locus_tag="ACHAT0_001624"
     CDS             10210..11571
                     /locus_tag="ACHAT0_001624"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8451568.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11531..12682
                     /locus_tag="ACHAT0_001625"
     CDS             11531..12682
                     /locus_tag="ACHAT0_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8451569.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12657..13826
                     /locus_tag="ACHAT0_001626"
     CDS             12657..13826
                     /locus_tag="ACHAT0_001626"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8451570.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14001..15380
                     /locus_tag="ACHAT0_001627"
     CDS             14001..15380
                     /locus_tag="ACHAT0_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8451571.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         5052 a         2734 c         3297 g         4404 t
ORIGIN      
        1 gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac ggattcaatt
       61 gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac tttgccattt
      121 ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt accaggagct
      181 tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc aatcaattga
      241 tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc agtcgctgaa
      301 tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc ttgtgtctga
      361 taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca ttttgttgca
      421 gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac agtgccgtct
      481 tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta ttttatataa
      541 taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac tcttttcaga
      601 caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca tctttacgtt
      661 cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa aattcacgag
      721 ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt gtttcattgc
      781 ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga acatctaaaa
      841 tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg catcccattg
      901 gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc aagtgctcaa
      961 gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag cggacgtcgt
     1021 atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt acataaggtg
     1081 ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt tgtaagactc
     1141 ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag tgaaatttac
     1201 aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc aatctgaaat
     1261 attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga ttctgtcaaa
     1321 tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag aaagacggat
     1381 caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg catgagtcat
     1441 gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta aagtaaatag
     1501 ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa aagaaatcat
     1561 tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat cgggtaatcc
     1621 tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta ccacaaaagc
     1681 atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct gaagtaacca
     1741 gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga tctgatcggc
     1801 tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc cgcctagata
     1861 tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt gaaaataagg
     1921 agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca gagtggcact
     1981 tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat aaagtgcttt
     2041 tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta tgatcgtgta
     2101 ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc catagacatc
     2161 atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat gaatcccaga
     2221 aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa tcagttcttc
     2281 caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag gctggccgac
     2341 gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc ctgttgtggg
     2401 atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc tttcctccta
     2461 taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg taacgacacg
     2521 atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg caccactcga
     2581 acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa acccttctac
     2641 atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg gaatgaattc
     2701 tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca cagagccctc
     2761 tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg tagaagttcc
     2821 agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt ctttgatttc
     2881 atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt tattttcaaa
     2941 ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt tgctgatggc
     3001 accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg ctttcatcaa
     3061 ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta atttgtatgt
     3121 gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga tgatcgtcgt
     3181 tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc ccaatctatc
     3241 tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag aacctttagg
     3301 catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag cttctgtaat
     3361 tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag ctgactatta
     3421 aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa gttacctggt
     3481 actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt ttatgcacca
     3541 tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc cctccaagta
     3601 aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa agaaaaaaat
     3661 atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg agaccttaaa
     3721 agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt gattatgata
     3781 aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg gtatgacttt
     3841 cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac ctggtaaagg
     3901 aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat acgacacaac
     3961 gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg tacaatattt
     4021 gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc aatatgaaat
     4081 tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg aagtaaaaat
     4141 ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg tattacgtgt
     4201 tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta agccagcgac
     4261 aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg aattgttgga
     4321 aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg ctaacggatt
     4381 gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga tctgcctttc
     4441 aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat tttatttata
     4501 ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt tatgaagaaa
     4561 ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt gaagcgaaaa
     4621 tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata gcttctaata
     4681 gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa tatttcgaaa
     4741 tatactagac aattcagaaa attttgttta atattattcc aataaaattc aaatctttat
     4801 caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa aatgacagaa
     4861 ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac acctctgtat
     4921 gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg ctttgtatcg
     4981 aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat tgagatgctg
     5041 catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga aatctatacc
     5101 gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa caagacgcca
     5161 gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga taatttgatg
     5221 gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg tatcatgctt
     5281 cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc gcatattgat
     5341 tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca gttgataaaa
     5401 gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca aatttttgat
     5461 gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga ttttgaccaa
     5521 cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa tgatcagccg
     5581 atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc tttggaaaaa
     5641 cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt tggagaagct
     5701 ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa atattacttt
     5761 gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga atacgattgt
     5821 gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc aggaaaaatg
     5881 tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga atcgggtgat
     5941 cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa ctacaatcga
     6001 gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat tgttcgcaga
     6061 caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata aatataatgg
     6121 ctgcggaaat gattttatct tattagatta cgcagaagac acagattata gtcaattagc
     6181 cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg taaaaatcga
     6241 tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt gcggaaatgg
     6301 cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac aggaattcaa
     6361 cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac ctttttattg
     6421 cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta atcaaactta
     6481 tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca ctagtttatt
     6541 tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag tctggagaaa
     6601 aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg tcaactttgt
     6661 agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag taggttggac
     6721 acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa ctgggaaaat
     6781 ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct ctggtgagga
     6841 tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg aggaatgaag
     6901 atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg atcagtagat
     6961 tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga tggattatta
     7021 gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga gcagatcctt
     7081 caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc tggaacgaat
     7141 aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc agacgcactt
     7201 ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc tcattttaca
     7261 gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc tagaacaggc
     7321 atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat cattggtttg
     7381 aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat tggagaagaa
     7441 ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt aggaggaagc
     7501 ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt agtaaaagat
     7561 acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact tgaactgatc
     7621 catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc attattagac
     7681 ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa aaagaaacaa
     7741 ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga ctgattggca
     7801 gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat acggtccttc
     7861 ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg ccagaactgc
     7921 ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc ttatcatatc
     7981 aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa gaaataaaag
     8041 aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc atcatggtga
     8101 tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc gaactgattg
     8161 aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg ctgcttcaaa
     8221 caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa aaaagagaag
     8281 aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa catgatgtat
     8341 tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct aatcgaatct
     8401 ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca ggttattaca
     8461 aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc aagaaatcat
     8521 ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga atgtaaccga
     8581 gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg ttgtttttgg
     8641 tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca ttgaaatcga
     8701 aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa aaattaatgc
     8761 tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca atgccgtgat
     8821 catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga tgattgatat
     8881 gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg gtgcaggagc
     8941 tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag aagacggcgt
     9001 actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag atgcagtcgt
     9061 ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg gtggaatacc
     9121 tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat tgattgctgc
     9181 gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct gtattgtggg
     9241 ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg attttccagt
     9301 aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg tattcaagaa
     9361 aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg atttagcgct
     9421 attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa gtcagggact
     9481 atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat taatcgttcc
     9541 agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa attgttcaac
     9601 cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc agcgagtcaa
     9661 ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag atcttcatcg
     9721 tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa cggtgattcc
     9781 ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa tgataaatga
     9841 gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg tacgtgtgcc
     9901 aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt ttacgacgga
     9961 agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg atctagcagc
    10021 acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg ttgggaggat
    10081 cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg ataacatacg
    10141 caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga aaatagcgga
    10201 ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc gatgtctcat
    10261 gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg ccaagtgatc
    10321 gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga cttactttat
    10381 ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa aaggatttgt
    10441 gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga agatgaacta
    10501 gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt agtaagtaga
    10561 ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt tattgacgca
    10621 aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac agcacaggaa
    10681 atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta cggggcgaac
    10741 cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg cgcgatactg
    10801 gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg aatcatgatg
    10861 gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt tagagaactg
    10921 agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt cccagttcaa
    10981 gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca agggacaaaa
    11041 atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg acgcaaggat
    11101 tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt tatctggaaa
    11161 gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc agggatcgac
    11221 aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct catcacgaaa
    11281 gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt agctttgatc
    11341 tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt gttatcgata
    11401 ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga gttgaatctg
    11461 atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta tgaagggatg
    11521 gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg aaatcgggtt
    11581 tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg aggccgttat
    11641 ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag aagaaacgat
    11701 tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc tctcttttgc
    11761 ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg cgatgctttt
    11821 agggttgact gactttataa ctaaacacat tgaggatctt atttataatg tagtactgat
    11881 ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt caggattgtt
    11941 ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac tagctaaggg
    12001 agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag acattatcat
    12061 taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc tggaggcagc
    12121 tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag agtggaatca
    12181 tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag ctggagaagt
    12241 tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt tgaagagggg
    12301 attagaccat ttagcagaac agtttcactc tacagctact atttcttttc gctataatga
    12361 cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag attcaaacaa
    12421 actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat atacgcaaca
    12481 atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac atgatccgac
    12541 gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa cgattctttt
    12601 tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag taaataatga
    12661 tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca aaaatacgac
    12721 aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg ggagaaccgg
    12781 attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa gaagactttt
    12841 ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt atatttcagc
    12901 aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca gttggtgcaa
    12961 cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag gttcttattc
    13021 cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct gaattgatcc
    13081 cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa cgagcatttg
    13141 aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat cctactggga
    13201 cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa tatccggtat
    13261 ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat atgtccatag
    13321 ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca catgcgatga
    13381 ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa ctaattaaag
    13441 tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg gctgaagcgc
    13501 taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca cgaagagact
    13561 atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac ggtgcatttt
    13621 acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt tgtgtagaat
    13681 tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca gaaggagaag
    13741 gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc tgcaaacgga
    13801 tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca atcgttaatt
    13861 ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat gaacgcgttt
    13921 cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat tttaaaagaa
    13981 tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt gcatctcatg
    14041 gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta gatatcacgc
    14101 tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg tatttagaag
    14161 acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt ccgcaatcca
    14221 atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa aaaacagtaa
    14281 cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa ttgattttga
    14341 gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa aatgtctact
    14401 tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat ccggctatta
    14461 aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa gcaagccgaa
    14521 aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc acttatttag
    14581 atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt gaagttttga
    14641 caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc aaaacaagtg
    14701 aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca aatacagaat
    14761 ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat gaatacttgc
    14821 aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat tctatcccag
    14881 cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca cgttatgtag
    14941 taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta ggttcctctg
    15001 cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt acagcagcaa
    15061 aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca aaatatgtac
    15121 cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca atgacccatc
    15181 aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga aatgtattgg
    15241 cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac ttcttcttcc
    15301 agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa gcgttaggag
    15361 aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga aaaagctgcc
    15421 ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt aacttggctg
    15481 gacttat
//
LOCUS       DAYYAR010000061        15255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000061 DAYYAR010000000
VERSION     DAYYAR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.061"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="ACHAT0_001628"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="ACHAT0_001628"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8451572.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /gene="rpoZ"
                     /locus_tag="ACHAT0_001629"
     CDS             952..1257
                     /gene="rpoZ"
                     /locus_tag="ACHAT0_001629"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8451573.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="ACHAT0_001630"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="ACHAT0_001630"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8451574.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="ACHAT0_001631"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="ACHAT0_001631"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8451575.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /gene="fmt"
                     /locus_tag="ACHAT0_001632"
     CDS             4417..5355
                     /gene="fmt"
                     /locus_tag="ACHAT0_001632"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8451576.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="ACHAT0_001633"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="ACHAT0_001633"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8451577.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="ACHAT0_001634"
     CDS             6726..7466
                     /locus_tag="ACHAT0_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8451578.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="ACHAT0_001635"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="ACHAT0_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8451579.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="ACHAT0_001636"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="ACHAT0_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8451580.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /gene="rpe"
                     /locus_tag="ACHAT0_001637"
     CDS             10609..11259
                     /gene="rpe"
                     /locus_tag="ACHAT0_001637"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8451581.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="ACHAT0_001638"
     CDS             11260..11898
                     /locus_tag="ACHAT0_001638"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8451582.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="ACHAT0_001639"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="ACHAT0_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8451583.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..13420
                     /gene="mreD"
                     /locus_tag="ACHAT0_001640"
     CDS             12911..13420
                     /gene="mreD"
                     /locus_tag="ACHAT0_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8451584.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13675..15249
                     /locus_tag="ACHAT0_001641"
     CDS             13675..15249
                     /locus_tag="ACHAT0_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8451585.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         5114 a         2864 c         3319 g         3958 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aattattcag tgatgaataa tttctttttt tatttttagg
    13441 tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct agacttaacg cggctttttc
    13501 atgatcattt tctgaaacga acagttttgt catttttaaa agttgttttg aaacattttt
    13561 gtaacataac tattatatcg ttgacacact gctatggtac aatttttata tcgttaagaa
    13621 agacttaaat atatttagat atttattaaa ttaagccagt cggaggaatg aagagtgaaa
    13681 aagagtttaa tatcagcagt aatggtatgt tcaatgacct tgactgccgt agcatcaccg
    13741 atcgctgccg cggcggacga ttttgattct cagatacaac agcaagatca aaagattgca
    13801 gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg atgccttaga aagccaagtt
    13861 tctgaaatca acacacaagc acaagaccta ttagcaaaac aagatacatt gcgtcaagaa
    13921 tctgcacaat tagtaaaaga cattgcagat ttacaagaac gtattgaaaa acgtgaagat
    13981 acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca caagctcaaa ctacatcgat
    14041 gctgtattga atgctgattc attagcagat gctattggac gcgttcaagc aatgacaacg
    14101 atggtaaaag caaacaacga cttgatggaa caacaaaaac aagataaaaa agctgtagaa
    14161 gacaaaaaag ctgaaaacga tgctaaacta aaagaattag ctgaaaacca agctgctttg
    14221 gaatctcaaa aaggcgatct tttatctaaa caagctgatt tgaacgtact gaaaacttca
    14281 ttagctgctg aacaagcaac tgctgaagac aagaaagctg acttgaaccg tcaaaaagca
    14341 gaagctgaag cagagcaagc tcgtattcgt gaacaacaac gtctagcaga acaagctcgt
    14401 caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg aacaagcaga ggctgaagca
    14461 caagctactc aagcttcatc tactgcacaa agctcagcaa cagaagaatc atcagctaca
    14521 caatcttcaa tgactgaaga atcatcatca gctacacaat cttcagcaac agaagaatca
    14581 acaacacctg aatcttcaac agaagaatca acagcgcctg aatcttcagc aacagaagaa
    14641 tcaacaacag cgcctgaatc ttcagcaaca gaagaatcaa caacagtgcc tgaatcttca
    14701 gcaacagaag aatcaacaac agtgcctgaa tcttcaacaa cagaagaatc aacaacacct
    14761 gcgcctacaa caccatcaac agatcaaagt gttgatacag gaaacggcac aggaagtagt
    14821 actccggctc caacgccaac accaacacct gaacaaccaa aacctgtaac acctgctcca
    14881 gctccttctg gaagtgtgaa tggtgcagca atcgtagcag aagcttacaa atatattggt
    14941 actccatatg tttggggcgg taaagatcca agtggctttg actgctcagg gttcacacgc
    15001 tatgtttaca tgcaagtaac tggtcgtgac atcggtggtt ggacagttcc tcaagaatca
    15061 gctggaacaa aaatttcagt ttctcaagca aaagctggag acttgttatt ctgggggtca
    15121 caaggcggaa cttaccacgt agcgattgct ttaggcggag gacaatatat ccatgctcct
    15181 caaccaggcg aaagcgtaaa agttggttca gttcaatggt ttgcccctga ctttgctgtc
    15241 agcatgtaag aatga
//
LOCUS       DAYYAR010000062        15215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000062 DAYYAR010000000
VERSION     DAYYAR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.062"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(306..378)
                     /locus_tag="ACHAT0_001642"
     tRNA            complement(306..378)
                     /locus_tag="ACHAT0_001642"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(343..345),aa:Lys,seq:ctt)
     gene            759..1328
                     /locus_tag="ACHAT0_001643"
     CDS             759..1328
                     /locus_tag="ACHAT0_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8451586.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1315..2175
                     /locus_tag="ACHAT0_001644"
     CDS             1315..2175
                     /locus_tag="ACHAT0_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8451587.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2365..3069
                     /gene="yycF"
                     /locus_tag="ACHAT0_001645"
     CDS             2365..3069
                     /gene="yycF"
                     /locus_tag="ACHAT0_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8451588.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3074..4909
                     /gene="walK"
                     /locus_tag="ACHAT0_001646"
     CDS             3074..4909
                     /gene="walK"
                     /locus_tag="ACHAT0_001646"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8451589.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4906..6222
                     /gene="yycH"
                     /locus_tag="ACHAT0_001647"
     CDS             4906..6222
                     /gene="yycH"
                     /locus_tag="ACHAT0_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8451590.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6223..7092
                     /locus_tag="ACHAT0_001648"
     CDS             6223..7092
                     /locus_tag="ACHAT0_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8451591.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7147..7956
                     /locus_tag="ACHAT0_001649"
     CDS             7147..7956
                     /locus_tag="ACHAT0_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8451592.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8059..8688)
                     /locus_tag="ACHAT0_001650"
     CDS             complement(8059..8688)
                     /locus_tag="ACHAT0_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8451593.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8858..10150
                     /locus_tag="ACHAT0_001651"
     CDS             8858..10150
                     /locus_tag="ACHAT0_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8451594.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10226..10888)
                     /locus_tag="ACHAT0_001652"
     CDS             complement(10226..10888)
                     /locus_tag="ACHAT0_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8451595.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11113..11397
                     /gene="groES"
                     /locus_tag="ACHAT0_001653"
     CDS             11113..11397
                     /gene="groES"
                     /locus_tag="ACHAT0_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8451596.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11448..13073
                     /gene="groL"
                     /locus_tag="ACHAT0_001654"
     CDS             11448..13073
                     /gene="groL"
                     /locus_tag="ACHAT0_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8451597.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13162..14301)
                     /locus_tag="ACHAT0_001655"
     CDS             complement(13162..14301)
                     /locus_tag="ACHAT0_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8451598.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKMIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYGEGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLSNRAKELIESVIA
                     DNILAGRPTNPDQYIFISTNGTPLTLHSFNAVLHKVEEELELEKNLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKKAKQQVIDKLNRL"
     gene            complement(14412..14996)
                     /locus_tag="ACHAT0_001656"
     CDS             complement(14412..14996)
                     /locus_tag="ACHAT0_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8451599.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15123..>15215)
                     /locus_tag="ACHAT0_001657"
     CDS             complement(15123..>15215)
                     /locus_tag="ACHAT0_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFM8451600.1"
                     /translation="YSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         4927 a         2616 c         3158 g         4514 t
ORIGIN      
        1 aatttagttt ttctattaaa tattcttcac tttttaacct tttatagtac ttagatttaa
       61 tcctatatgt ttagagagtt cattggcagc atgattttgc aaagcttctc gaattattgt
      121 tatattagaa actttactgt tcataattat gcttcttttt taactcaaag tccaaagggc
      181 tacagtggac tttgagttaa aaataaaaag ccctagttat tttgaaaaat aaaaaaagaa
      241 cagcgtaaat gttttcgaaa tgctttcgca ccaacattta cctgttccgt gggtttggca
      301 cccaatgggc cgtgaggggc tcgaacccgc gacccgctga ttaagagtca gctgctctac
      361 caactgagct aacggcccgt acaaaaagta cttaataata gtagcactaa gaattttaaa
      421 atgcaagact acatttaaag aaaatcagaa aaatctttac ttccttatag gaaatcattc
      481 atagattatt cacatttgct cgctattata taagcatacc aacaaacaaa gttcttgcaa
      541 gagaacaaac cctaacactt ttttcatttt ttactccttt cccgccgtct ccccggcggg
      601 tctcctgttc ttaacgataa aagacaagaa ggcttagcct tcttgttttt tgttttcatg
      661 gaatcccgct aatcaaatca cctagttaaa gcaactagat tgaatgttta tcataagtct
      721 gatatcatgt ttatgaaatg aaaaaaggag cggttatgat ggaaaaaatg cgtcagcaat
      781 taatggagtg ggggaaggaa ctagaaacat tccgtctgcc tcactgggaa gaactccctg
      841 acttagaact ttatatggac caagtcagaa cgttagttga tcgttatctt tcacctgtga
      901 tacaaggaga aaagcatcca ttgcttacct cttctatggt aaacaattac gttaaactag
      961 gtttgatacc agcaccagtt aaaaaaagat ataacaaaga acacgtagca tttttacttg
     1021 caatcactac gttgaaacaa gtattgacta ttcctgaaat caaagaaggg atattgttcc
     1081 aaggaaaaac agtaggaatt cgtgaagcct ataatctatt ttgcgatgaa caagaagcag
     1141 ctgtgtggat ggtcagccaa ttagcacaag gtaaatctca tccgcaaaaa ttcgaccagg
     1201 caacccctgt agaatatatt gctgtaagag cggcaacttt atcgtttgca atgaaacttc
     1261 ttgcggaaaa aacgattgtt ctagaaaccg aatatttaaa agaggagaaa acaaatgaaa
     1321 aacaataaaa tcgcaatttt agtagattca ggtacggatg ttccagaaga attgataaaa
     1381 aaatacgaga tgtatatgat cccattgaaa attatttata aagatcaggt atatacagat
     1441 aaaattgaca ttacccctga agagatctat caacgtttgc cagaggaaat ccctggtaca
     1501 tctttgccgg atggtgagtc gatcacagaa atctttgagc aaatcaaaaa agacggttat
     1561 gaaaaggtac ttgcggtaac aatttccagt ggattgagcg ggacatataa tgtggttcgt
     1621 ttaattgcac aacagcaaga agaattggaa gcatatgtac tagatacgaa aaatattggt
     1681 attggtgcag gtttttctgc gatccaagca gcaaaatggc tggaagaagg agtagaatgg
     1741 gatcaactga tcagtaatct gaatgagtta gtcgaaagaa caaaagtttt ctttaatgtt
     1801 gcgacattgg aatatcttca aaaaggtgga cggatcggtt tagttgcttc tattgtagga
     1861 accgctttaa agcttaaccc aatcatctca tgtaatgaag aagggattta ttacacagta
     1921 ggaaaagcta ggggacgtaa aaaaagtttg gataaaacgg ttgcttacgt caaagaaaaa
     1981 atcggtaatg caaaggtctt caacttagct gttgcccatg gggatgcgaa agaagaagca
     2041 attgaaatgg aagcgagact taaaagagag ttcccacaag caaaccaaat ctactttggc
     2101 cagatttctc cggcattagt agtccacact ggtccgggat tattgggtgt cggtgtacaa
     2161 gttttagaag gataaatcaa aaaaaggaaa cactgaggtg aaaatcagtg ttttcctttt
     2221 ttattgaagt tagaacaaaa aataataaag tattttgcac tttcatttta attttctctt
     2281 ttgttttata ggcaaagatg aaacaaaagc agaaatacgg tataatgaga aggtatgaca
     2341 ataatatata agaggtggct ctttatgaaa aaagttttag ttgtcgatga tgagaagccg
     2401 atttcggata tcgtcaaatt caatttagcc aaagaaggct atgacgttta tactgcatat
     2461 gatggggaag aagccttgga aaaagttgct gaggtagaac ctgatttgat tttacttgat
     2521 ttgatgctac ctaaaatgga cggcttagaa gttgctcgtg aggtacgcaa aacttatgat
     2581 atgccgatca ttatggtaac agccaaagat tctgaaattg ataaagtcct aggcttagag
     2641 ttaggcgccg atgattacgt gaccaagcca ttttccaacc gagaattagt tgcacgtgtc
     2701 aaagcaaatc ttcgtcgcgg tgccacggct gcgaaggaac cagaagaggc agcacctgcg
     2761 gaattgacga ttggtgattt gacgatccat ccagaagctt acatggtgac gaaacgagga
     2821 gaaacaatcg agttaacaca tcgtgaattc gaactactct tttacttggc gaagcacctt
     2881 ggacaagtga tgacaagaga acatttatta caaacggttt ggggttatga ttattttgga
     2941 gatgtacgaa cagtggatgt aactgtacgt cgtttgcgtg aaaagatcga agataatcca
     3001 agccatccaa attatttagt gacacgtcgt ggtgtcggtt actatttgcg taacccagaa
     3061 caggagtaat tggatgaaaa gaaaagtccg cttttttcga tcagtcaatt ttaagatcgc
     3121 tattaccttt attctgattt tattgatctc gatcgaaatt attggtgcct actttatacg
     3181 tggtttagaa cgttccacga tcaatacatt tattaaagat atgaatcaaa cggtggaatc
     3241 attagcaaca acaatcagcc cggaattgaa tcgaaaagat aatgcagatg atgaagaggt
     3301 aaatgcgaat atcaaaagat ttattgaaaa cagtgcgact agcgatatca tcgaaatacg
     3361 ggtcgttgac gaaaaaggga tcatccgagg aacaaccgat gtcaatgaac aaagcgctgt
     3421 gggaaagaaa aatgattatg ttgatatcaa tgatttcacg actaaacgtt atgttgcttt
     3481 agataacgac gataaacggg tgaatatcaa tgttcaaccg atcctgtccc cgaccggcga
     3541 tgccgttatc ggggctcttt acgtcaaaag taatatcgaa caaaaatatt cggaaatcaa
     3601 taacacagca gtcattttct ttactgcctc actaattgcg gcatttattt caatgatcgt
     3661 atctgtttta gttgctcgtt cgattacaca gccgatcggt gaaatgagag aacaagccat
     3721 tcgaatcgct agaggagact acagtcggaa agtcaaagtg catggacaag atgaacttgg
     3781 acagctggct gataccttca atcagttagc tgaacgtatt gaagaagctc aagatacaat
     3841 ggaagctgaa cgcaatcgtt tggatagcgt gctatcccat atgacagacg gcgtcattgc
     3901 gacagatcgc aggggtaaag tcattacgat caatgatatg gctgtttcct tgctagatat
     3961 caaaaacgag gaagcaatcg gacaatcgat tttgacactt ttagatatcg aagaagaata
     4021 tacgttgcgg aaattattag aagatccaaa cgaaatggta cttgaccgtt caaccagtac
     4081 attggaaagt gatcagatga tcttgcgagt agacttttct atgatccgtc gcgaatcagg
     4141 ttttatcagc ggattagtag ctgtcttgca tgatgtgaca gaacaagaaa aaacagaacg
     4201 ggaacgtcgt gaattcgtct ctaatgtttc ccatgaactt cgtacaccgt tgacaagtat
     4261 gcgtagttat atcgaagcat tgagcgaagg tgcgtggaaa gacgaggaag tagcaccaaa
     4321 ttttttgaag gtaacgctag atgaaacaga ccgaatgatt cgaatgatca atgacctctt
     4381 aaatctatct cgcatggata caggtaatac ccagcttcaa ttagaatatg tcaatttcaa
     4441 cgagttagtg aattttgttc ttgaccggtt tgatatgatg gtgggaaatc aggaaaaaaa
     4501 ttataagatc cgtcgtgaat ttacgcaaag agatctttgg gtcgaagtag atacagataa
     4561 aatcatccaa gtagtcgata atatcatgaa taacgcgatc aagtattcac cagatggggg
     4621 aacgattact tgtcgcttat tagaaacgca taataacgtg attttaagta tcaccgatca
     4681 aggattgggg attcctaaaa aagatctgaa ccgagtgttc gagcggttct atcgagtaga
     4741 taaagcacgt gcaagagcac aaggtgggac aggacttgga ttagctattt ctagagaagt
     4801 gattaaggcg catcgaggcg ctatttgggc agagagcaag gaaggaaaag ggtcgacttt
     4861 ttatatctcg ctgccatatg aaccttatga ggaggaatgg tgggaatgag aatctctgaa
     4921 aaagtcgttc gtataggact cattttgatg gttgcgttaa gcatatactt ctcttatgca
     4981 atctggctga gcccagcagg aaaaagttca atcaacgtag atgagacaaa cactcaaatg
     5041 atcgagtctc aaagcaatcg aaaaacttcc gaagtctttc ttccgcttca tgttacatgg
     5101 caccattcgg gaatgatcca agaaaccaac agtgaaaatc tcgtgtaccg attgcaattg
     5161 atcatcgata aagcaagatt tggaaagctt tctgaagtag tttcaggaga caaatcacag
     5221 tttgaacagg tgaaaaatct gaactctggt tttgagttgg cctataatgc gccttttttg
     5281 ctaagagaat acaaggaagc atttgattta gatttagatg tgtcgaactt ggcaaaagaa
     5341 agcgaaaata tgtattttac tagaatacaa ttcgatcaag gaaataaaaa aatccgattt
     5401 ttgaattatt cgaaccatct cgtatatgaa gcaaatattt cgttgaattg gacagatatc
     5461 aataaagaat taacagcaaa agacacaaaa tggacagaaa tgacaacaga accatcgacg
     5521 atcgacacgc aatatgctac gaaacattcg atcaaactga agaagtatag ttatatcctg
     5581 tcacagcaac cctatacact ttttagaaat gcttttttcc agaaaccaga tagcgtgaaa
     5641 aataatgatg aaagcaatga attgattttt tacggtggga atgaaacaat gtctatccat
     5701 tcggaaacgc aagtagttga ttttcgtgga gagctgccag agaaaaaaga aaatgctagt
     5761 gtattttctg aaagtttccc ctatattagc aaattaggca gttctttagg gaacctcaga
     5821 tattttgacc gcagtgacaa tacgatcgat tatcggatat tcgtggaagg cttcccggtt
     5881 tttgggacag acagcagagg acagattggt gtggaagttg gcggtgaaac gaatggttct
     5941 gtgcaagtga atatccaaac cagtttgaat acgattcaag tgccgatacc ttcagacgaa
     6001 gaagtagaac ttgcaagtac gaatgaaatc atccagcagt taaaagctaa gggagcagat
     6061 agcaacaaga ttggttcagt gatcgttggc tatacatggc aaaatgtcaa agaaacgaat
     6121 cgtgtcgttg atctcttacc tgaatggtat gtgaaatata aagataactg gtattcagct
     6181 aacgaccttc tggaaaaatt acctggattg gaggcgaatt aattggattt caaaaaaatc
     6241 gaatggatat ttttcgtcgc ttttttaggg ttgaatgtgt ttttattcag cagttatcat
     6301 gacgcggtat accaagaaaa caatgtcatt cgctctaatc agactgaatc aatccaacaa
     6361 cgtctggcta gcgacgatat cacttacacg gggaagattt cgtctgagaa caagcaaggc
     6421 tattatctta gcgcggagca gaccaatctt agcacttcat tagcgaatta tcgcaaggct
     6481 ggtaaaagcg gcttgctggc aagtggtgtg tttttagacg gtgatgtttt gacgaaatct
     6541 ttatccagtt cagatgctga aaagaacgtg atcgatccag ataaaattag tagttccttg
     6601 gaagattttt tatctaacaa agacaatatt ttattcggta atgattatgt gtacacgaaa
     6661 aacttttctc atttagaaga agaaccacta gaaatccaag caagccaaga gtatgaaggc
     6721 atcccattca atgatgatac agcaaaaatc gttctttctt tagaaaaaaa cgataatgat
     6781 tatagcatca cgaaatacat gcagacgcat ttatctgata tcgagcagct gcgcgaaaag
     6841 acggaactgt atacggaaga ggaagcaatc aatacattgt acatcaataa caaaatacca
     6901 agaggatcaa aaattttatg gcgtcagcta gcttattcct gtattctgaa agtccgggaa
     6961 aaaaatgtgt atgttcctgt ttggcatgta gcgatcgaaa caagcgataa agtggtccaa
     7021 attgagagcg tcaacgcttt cagcaatacg atcgtgacaa acaatactgt accaaaagtg
     7081 gaagatcaat aaaaaagcga gtataatgaa aaaagtttac aaagtaagaa gggaagtagg
     7141 agtcaaatgg ctgaagaaaa accagctttt aaaatcagta tcctagcaag tggaagttcg
     7201 ggtaattcac tttatatcga aagtgaaaaa aaacggcttt tggtcgatgc aggacttagc
     7261 ggtaaaaaaa tcacttcctt gatggctcaa attgatcgga caccggacca attagatgga
     7321 atacttgtga cgcatgaaca tcgagatcat atacatggcg tgggtgtctt ggctcgtaag
     7381 tatcatttgg atgtttttgc caatcagcag acttgggaag cgatggaacc actgattgga
     7441 aatgtaccag tggaacaaaa acatctattt gagatgggga aagtcagaac attcggcgat
     7501 atcgatattg agagtttcgg cgtctcccat gacgcagcgg ctccgcaatt ttatcgtttc
     7561 cataaagaaa atcggtcatt cgtcatgttg acggacaccg gatattgcag tgattatatg
     7621 cgaggtgtca ttgaaaatgc cgatggttat ttgatggaaa gtaatcatga tcttgagatg
     7681 ttacgcatgg gaccttatcc gtggaattta aaacagcgta ttttaggaga tagaggacat
     7741 ctttccaacg aagacggtgc acttgtcatg actgatgtga tcggtaatcg gaccaaacga
     7801 atctatcttg gccatcttag caaggaaaac aatatgaaag aacttgctca tttaacaatg
     7861 gaaaatgttt tgaaagaaaa agattttggc gttggacatg attttgaaat ctatgataca
     7921 gatccagatg aagcaactga gctattttcg atttaaacag ttcaatcaat aaaaatataa
     7981 aaagcagaag ataaaatcaa tcagggttgg ttttatcttc tatttttatt tttttagcat
     8041 attaaaaaaa taaccgtatt atcgactgac tacactgatg tgctgctttt catgtttctc
     8101 atccagtatc tcatctacta atgcaatcgc gtaatctgcc atgctgatcg tactgtcgct
     8161 agcagcgttt ctttgtaaat aatcatcact taacaaataa gtacctgtac gtttgccatg
     8221 tggatcaaat aaagaagctg gactgatata agtccaatgt agatttttag attgtttcaa
     8281 ttttaacaat ccttcgtaca tattgaaagc tgttggatag aagggtgcat ttttaggtac
     8341 ttggtcaatc atacgagtcg tcttttctgt atctaagaat aacgaagagg cacccccaac
     8401 gacaattaag cgtgtatcgg ttccttcaag catctcaatc aaatgggaca aagaagtttg
     8461 atgcatttct tcttttcctt tgggagcgtt gaaagcatca acgacaacat caaaagatgc
     8521 aagatcagta gttgtcaact caaataaatc cttttgtata aacggaacat ctgatggcaa
     8581 tttttctgga tgacgcacga ttgccgtcac gtctaaatct ctggacaatg cttcgtctaa
     8641 aataaatgaa cctgcatggc cagtagctcc aatgagtgca attttcataa aaaacctcct
     8701 tttttattcc tgtccattgt aagcagttgc tgaatattcg tcaaagaatc cgatttttga
     8761 aaagctcagt gcgcattcct ttcatggttc gtgatcattg ggtatagtta aattaagaaa
     8821 actgattgat aaaaatagaa gttgaaggag ataagaaatg aaagagtggt tagatcgtgc
     8881 ctttttacca atgataacat ctgttgtgaa tcttagaccc ttaactgctt tgcgtaatgg
     8941 aatgacttac acgatcccaa taattattgt tggctcgctg tttttcttat ttgctcattt
     9001 tcctgtggat tacatcgctc agtggataga caaaacagga ttttctatgt attttacaca
     9061 agtataccag gctacatttg atgtgatagc cttttggatc gtttttgcca ttgcatacgc
     9121 ttatgcaaaa gaagaagaca tcgatcgctt accagtcggc ataacagcct tgtctgctta
     9181 tctactgctt attcagccac taaatactca gatagatact gtctggacag gtgctgaagg
     9241 gatactagga gcagttacaa ctgggctcat catcggatgg ggctatgcac gtttagtaag
     9301 acttgcgaaa gggtggaagc aaagagagaa gatacctgaa aatgtttggc agtcttttac
     9361 ttcattgatt ccaatcattg ttatagtcac ttttacgctt atcggttcag cggtttttca
     9421 acatttgttc agaatgactg ttgttcaagg agtatggaca tatgtccagc ttcccattca
     9481 atcgttcata gacacgttgt ttggtgtgct tttattaggt ttttttgttc cgtttttatg
     9541 gttatttggt cttcatggat ctgcgatggt caatgggttg gttagtcccg tcttgcaagc
     9601 gaattcatta gcaaatgcag aaatcttagc ctcaggacaa gagttgacgg tggcaaatgg
     9661 cggacatatc gttactcagc aatttctcga tcagttcatg acggttacgg gtgctggatt
     9721 gacgttaggg gctgtatttt ttatgacggt ttttgcgaaa tcgaagaaat acagagaatt
     9781 aggaaatctt gctcttttac cagccttttt caacatcaat gaatcaatta ttttttccac
     9841 accgatcgtg atgaacccga tgatggctgt tccttttatt ttcgcaccgg tcgtttcagg
     9901 attgattacc tattccgctt tatattttgg attcgttcct ttatttacag cagtccaagt
     9961 cccttggaca acaccgccga tcctctcggg tttcttagta ggcggagtgc ctgcagcgat
    10021 tttacaaggt atcgtgttgt caattagttt tttcatttat ttcccatttt tgaaaaaaat
    10081 agattctgca aactgcaaaa aggaaagaca gacaaaaaat gatggagcag cagaaaagat
    10141 gaacgactaa aaaacaaaaa tactcctgtc gctttagatt gggaaaatca ccaatctagg
    10201 atgacaggag tatttatagt catgtttata ctagcccttt ttggacagct aattgaacaa
    10261 gaacaactaa tgtgttcatt cctacgtgtg tgatcatcga tgtccagatc cgaccagttg
    10321 ctttgtactc taggctgaag aagaaaccta ggaaaaagta gattagcaga tgaccatcat
    10381 tatgagcaaa cgcaaacagc aaagagctga tcactgctcc gacccaaaaa tttgaatatc
    10441 gactgatgat ccccagtata ctgcggcgga atacgaactc ttccatgatg ggtccagcaa
    10501 cagttgtcgc caaaataaaa gcgggtgctt ccatgatcat ctggatgata ttttgtgtat
    10561 tctcggaagg ggttgctttg ccaaacaata cagattctaa catgacagcg atgctttgca
    10621 gaatcaatgc gacaaagatt ccaaccaatc cataaataac aatctttgga gcagaagctt
    10681 tttttaccgc tgattcgatc gctaagggtt cacgttgctt aaaataaaaa atagctagga
    10741 caacggcgcc tagtatatac atgactgcag ttgctgtgat caattcagac gtagatttta
    10801 caagtcctat cgaagctagg atcaagggtg agataaaaat tagaccatag caaaaaatgc
    10861 tgaggaagct atattttttt aaagacatgt aattcctcct tttaagggat aacaagcatg
    10921 caaggcaagc ttatatcgat tgatttcaat tatatcataa tttcaataag cagctgtcag
    10981 aataaaattt cttttctttg atttaaaagc ttgcaaaaaa gagggagaat gagtattatt
    11041 aaaaatgtag gttagcactc gaaggttaag agtgctaaca gattaaaaaa agagttatcg
    11101 gagggattta tcgtgttaaa accattaggt gatcgcgtca ttatcgaagt cgcaaaagaa
    11161 gaagaaaaaa ctgtcggagg aatcgtttta gcttctgctg ctaaagagaa accccaaact
    11221 gggacagttg ttgcagtcgg tgaaggccgt ttgttagaaa acggtgaaaa agttcctgcc
    11281 gctgtaaaag caggcgacca agttatgttc gaaaaatacg ccggtacaga agtaaaatac
    11341 gaaggaaaag aatatctgat cgtcgcagga aaagatatca tggcgattgt tgagtaaatt
    11401 tggaagataa aaaaataatc aaaaataatc aatgaggtga atcaatcatg gcaaaagaat
    11461 tgaaatttgc agaagacgca cgcgcagcta tgttacgcgg ggtagataaa ttagcagata
    11521 cagtgaaagt aacattagga cctaaaggcc gaaatgtcgt actagaaaaa tcttacggct
    11581 ctccattgat cacaaatgat ggtgtgacga tcgcaaaaga aatcgaatta gaagatcatt
    11641 ttgaaaacat gggtgcaaaa cttgtatcag aagtagcatc aaaaacaaat gatattgcag
    11701 gagacggtac aacgacagca actgttctga cacaagcaat cgttcgcgaa ggtttaaaaa
    11761 acgtaactgc aggtgctaat cctctaggca ttcgtcgcgg gatcgaattg gcaacaaaag
    11821 cagcagtcga agaattgcat aatatctcaa ctgttgttga ttcaaaagaa gccattgcac
    11881 aagtagcagc tgtttcttcg ggttctgata aagttggaca tttgattgct gatgcaatgg
    11941 aaaaagttgg aaacgacggt gtcatcacaa tcgaagaatc aaaagggatc gaaacagaac
    12001 tagatgttgt agaaggtatg cagtttgatc gtggttactt atcacaatat atggtaacag
    12061 ataacgacaa aatggaagct gttctagaaa acccatatat cttgatcaca gacaaaaaaa
    12121 tctctaacat ccaagatatt ctgccattgt tagaacaaat cttgcaacaa tcacgtccat
    12181 tgttgattat tgctgacgat gtagatggtg aagcattgcc aactcttgta ttgaacaaaa
    12241 tccgtggaac attcaacgta gtagctgtaa aagctcctgg tttcggcgat cgtcgtaaag
    12301 caatgcttga agatatcgct attttgacag gtggtacagt aattactgac gatctaggtt
    12361 tagaattgaa agatgcaaca atcgaaaact taggaaatgc ttctaaagtt gttgtagata
    12421 aagacaatac aacgatcgta gaaggttctg gagaaaaaga agcaatcgaa gctcgtgtgc
    12481 aattgatcaa aaaccaaatt gctgaaacaa cttctgattt tgaccgcgaa aaattacaag
    12541 aacgcttagc taaattagca ggtggtgtag ctgttgttaa agttggtgca gctactgaaa
    12601 cagaattgaa agaattaaaa ttacgcattg aagatgcatt gaatgctaca cgtgccgcag
    12661 tagaagaagg aatggtctca ggtggtggta cagctctagt gaacgttatc agcaaagttt
    12721 ctgctgtaga agcagaagga gacgtggcaa caggtatcaa gatcgttgtt cgtgctttag
    12781 aagaaccaat tcgtcaaatc gctgaaaacg ctggttatga aggatcagtg attgttgata
    12841 aattgaagaa cgtggaacta ggcactggtt tcaatgctgc tactggagaa tgggtaaaca
    12901 tggttgaagc aggtatcgta gacccaacta aagtaacacg ttctgccttg caaaatgccg
    12961 cttccgtttc tgccttgtta ttaacgacag aagcagtcgt tgcagataaa ccagaaccag
    13021 cagcgccagc agctcctgca atggatccat caatgatggg cggtatgatg taggaattaa
    13081 aagagaaccc ttatattaca gggttctctt ttcttttttt gttttatttt aagtaaaagg
    13141 ggcaaaaaag gggcagaaat atcaaagcct atttagttta tcaattactt gttgttttgc
    13201 ttttttggtt acgtgattgt aaatagacag agttgtattt gcatcagaat gtcctacacg
    13261 ctccataatg gctttaagag gtacgcctaa ttcagataat agcgaaacat ggctatgtct
    13321 aaatatatgc gatgatagat ttttttctaa ttccaactct tcttctactt tatggagtac
    13381 agcgttaaat gagtgcagcg taagcggagt gccatttgta gatataaata tatattgatc
    13441 gggattggtg ggtctacctg caagaatatt gtcagctatc acactttcaa tcaattcttt
    13501 tgcacgattg gataattgta cttcacgttg tgagtaagta ttttttggag ttgtttttat
    13561 ggcgttatcc atttttacag atgtgtaatc taaggtccca ttaatggaaa ttttaccctc
    13621 tccatagtcc ttcatttgca aagccagcaa ttccccatat ctcaaaccag tcaaatatag
    13681 aaattcagct attatgccgt gtagttttct gcgaggattg gaatataact gtttcagtat
    13741 ttgatcaatt tcttctttct ccagatattt tttatccata gaaagctttc ttttttcttc
    13801 ttccactttt ttaggatgga ttttaactgc tagagcaggg tttctttgaa tgtattttct
    13861 atcaattgca tagtttagca taacggatag agttgttttt gtttgctttg tgtagttcag
    13921 tgagagatca ccaaaagtgt acatatcttc aatcatctta ttaatgagtg tctcatcaat
    13981 gtttctaacg attgtatcat cgcttatgtg cttagaaaca tgtttcatca tcattggaac
    14041 cttcaaatag ctagtacgtt taacatgctg cttataatat tcataccatt ctttatacag
    14101 ctcaccaaaa gtgatatctg atttattgta attttcgagt gcttctttaa tttttttatc
    14161 tagaattttc tgagcttttt tccacgcttg tggtgaatta cttgtaagtg ttgtagattt
    14221 ttttcgtgtt ttttctgtat aaggatctac atatctttca ataaacttga attggccatc
    14281 tttggtttgt tcaacccaca cttttaacat ctcctatcat ttgctataat aggcataaca
    14341 aatagaccta tataggtttg ttttctaaaa gcacgctctt actctggacg gtggggcgtg
    14401 ttttttattt attatacagt tgctttaaaa atagctgctt tttcaaatga tactaaagga
    14461 gagaagtgga tttctatttc tcccttttgg tttaacgtga aatgagcagt tacatccatt
    14521 tgttttcctg gagcaacaga tcccattgta ttttcgttgg catatgtctc tgattttttg
    14581 tcatctggtc catatacttc cacatctgta cctacaggga tatctgaatc gccatcattt
    14641 tttacggtat aagtaatttt tactacttgt gcaggttggt tttcttcaaa ttgatttctt
    14701 tcatcagtta gttctacact atttagtgta tattcagcat caccaacttt tacagtgtca
    14761 ccaatcttat agaaggtatc gcttttttct tctttagaag atgaagcaga ggttgaagat
    14821 ttagttactt tttcgccacc attatcactt gctttattac tatctgatcc accatttaaa
    14881 gcagaaccaa taattataat taaaattact gctaataccc aaaaccatac ttttttgtaa
    14941 aaaggtttac taactttata catttttccg tcttgaccca taactttttt tgccatttaa
    15001 atattcctca cttcttgtta taatatattt gcgatctcag aaatgaggta tgagtccgtg
    15061 tgccagcacg ggctttttta atgttttgct gaaatcggtt tttttaataa ttcttgatgt
    15121 attcatacat attgccttgc gtaagaatat tttttcttaa aatagcattg gcagacagca
    15181 taacaagtgt atctgagctt attctgaata gaata
//
LOCUS       DAYYAR010000063        15138 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000063 DAYYAR010000000
VERSION     DAYYAR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.063"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACHAT0_001658"
     CDS             5..2377
                     /locus_tag="ACHAT0_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8451601.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACHAT0_001659"
     CDS             2438..3898
                     /locus_tag="ACHAT0_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8451602.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACHAT0_001660"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACHAT0_001660"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8451603.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACHAT0_001661"
     CDS             6129..6278
                     /locus_tag="ACHAT0_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451604.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="ACHAT0_001662"
     CDS             6353..6946
                     /locus_tag="ACHAT0_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8451605.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="ACHAT0_001663"
     CDS             6959..7858
                     /locus_tag="ACHAT0_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8451606.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="ACHAT0_001664"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="ACHAT0_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8451607.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="ACHAT0_001665"
     CDS             8724..8984
                     /locus_tag="ACHAT0_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451608.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="ACHAT0_001666"
     CDS             9429..9635
                     /locus_tag="ACHAT0_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8451609.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9656..9886
                     /locus_tag="ACHAT0_001667"
     CDS             9656..9886
                     /locus_tag="ACHAT0_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451610.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="ACHAT0_001668"
     CDS             9902..10339
                     /locus_tag="ACHAT0_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451611.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="ACHAT0_001669"
     CDS             10332..11039
                     /locus_tag="ACHAT0_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451612.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11203..11403
                     /locus_tag="ACHAT0_001670"
     CDS             11203..11403
                     /locus_tag="ACHAT0_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451613.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11419..11598
                     /locus_tag="ACHAT0_001671"
     CDS             11419..11598
                     /locus_tag="ACHAT0_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451614.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="ACHAT0_001672"
     CDS             11923..12186
                     /locus_tag="ACHAT0_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8451615.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="ACHAT0_001673"
     CDS             12180..12530
                     /locus_tag="ACHAT0_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8451616.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="ACHAT0_001674"
     CDS             complement(12554..13168)
                     /locus_tag="ACHAT0_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8451617.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13482..13904
                     /locus_tag="ACHAT0_001675"
     CDS             13482..13904
                     /locus_tag="ACHAT0_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451618.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13914..14117
                     /locus_tag="ACHAT0_001676"
     CDS             13914..14117
                     /locus_tag="ACHAT0_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451619.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14328..14912
                     /locus_tag="ACHAT0_001677"
     CDS             14328..14912
                     /locus_tag="ACHAT0_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8451620.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5570 a         2451 c         3061 g         4056 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt
    13321 ttaaatggca atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca
    13381 ttaaaaatat attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt
    13441 aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
    13501 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
    13561 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
    13621 ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
    13681 taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga
    13741 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
    13801 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
    13861 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
    13921 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
    13981 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
    14041 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
    14101 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
    14161 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
    14221 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
    14281 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
    14341 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
    14401 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
    14461 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
    14521 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
    14581 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
    14641 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
    14701 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
    14761 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
    14821 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
    14881 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
    14941 gccatttatc tagaaaagtg acaatagtat gagaattttt tgtaagtgtc tactgataac
    15001 ttattctcaa atgttgtaag gattttattc atttgttaat attaaagaac ggaactccaa
    15061 aaaagtccgt actctaattt gcagtctctc actcttttag agtgagagtt tttattagat
    15121 tttcaaatta attagtaa
//
LOCUS       DAYYAR010000064        14994 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000064 DAYYAR010000000
VERSION     DAYYAR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.064"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACHAT0_001678"
     CDS             <1..608
                     /locus_tag="ACHAT0_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451621.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="ACHAT0_001679"
     CDS             628..1656
                     /locus_tag="ACHAT0_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8451622.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="ACHAT0_001680"
     CDS             1653..2411
                     /locus_tag="ACHAT0_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8451623.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="ACHAT0_001681"
     CDS             complement(2497..3237)
                     /locus_tag="ACHAT0_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8451624.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="ACHAT0_001682"
     CDS             3298..4551
                     /locus_tag="ACHAT0_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8451625.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4846..5100
                     /locus_tag="ACHAT0_001683"
                     /pseudo
     CDS             4846..5100
                     /locus_tag="ACHAT0_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="ACHAT0_001684"
     CDS             5222..5548
                     /locus_tag="ACHAT0_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8451626.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="ACHAT0_001685"
     CDS             5616..6329
                     /locus_tag="ACHAT0_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8451627.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7491
                     /gene="licT"
                     /locus_tag="ACHAT0_001686"
                     /pseudo
     CDS             6639..7491
                     /gene="licT"
                     /locus_tag="ACHAT0_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7612..9477
                     /locus_tag="ACHAT0_001687"
     CDS             7612..9477
                     /locus_tag="ACHAT0_001687"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8451628.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9491..10934
                     /locus_tag="ACHAT0_001688"
                     /pseudo
     CDS             9491..10934
                     /locus_tag="ACHAT0_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11042..11743
                     /locus_tag="ACHAT0_001689"
     CDS             11042..11743
                     /locus_tag="ACHAT0_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8451629.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11869..12381
                     /locus_tag="ACHAT0_001690"
                     /pseudo
     CDS             11869..12381
                     /locus_tag="ACHAT0_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12779..12988)
                     /locus_tag="ACHAT0_001691"
     CDS             complement(12779..12988)
                     /locus_tag="ACHAT0_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8451630.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13229..13804
                     /locus_tag="ACHAT0_001692"
     CDS             13229..13804
                     /locus_tag="ACHAT0_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8451631.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13804..14244
                     /locus_tag="ACHAT0_001693"
     CDS             13804..14244
                     /locus_tag="ACHAT0_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8451632.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14260..14961
                     /locus_tag="ACHAT0_001694"
     CDS             14260..14961
                     /locus_tag="ACHAT0_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8451633.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5066 a         2485 c         3161 g         4282 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     6781 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     6841 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     6901 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     6961 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     7021 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     7081 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     7141 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     7201 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     7261 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     7321 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     7381 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     7441 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     7501 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     7561 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     7621 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     7681 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     7741 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     7801 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     7861 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     7921 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     7981 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     8041 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     8101 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     8161 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     8221 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     8281 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     8341 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     8401 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     8461 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     8521 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     8581 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     8641 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     8701 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     8761 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     8821 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     8881 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     8941 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     9001 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     9061 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     9121 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     9181 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     9241 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     9301 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     9361 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     9421 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     9481 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     9541 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
     9601 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
     9661 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
     9721 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
     9781 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
     9841 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
     9901 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
     9961 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
    10021 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
    10081 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
    10141 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
    10201 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
    10261 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
    10321 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
    10381 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
    10441 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
    10501 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
    10561 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
    10621 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
    10681 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
    10741 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
    10801 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
    10861 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
    10921 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
    10981 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
    11041 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
    11101 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
    11161 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
    11221 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
    11281 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
    11341 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
    11401 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
    11461 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
    11521 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
    11581 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
    11641 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
    11701 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
    11761 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    11821 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
    11881 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
    11941 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
    12001 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
    12061 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
    12121 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
    12181 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
    12241 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
    12301 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
    12361 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
    12421 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
    12481 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
    12541 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
    12601 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
    12661 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
    12721 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
    12781 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
    12841 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
    12901 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
    12961 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcccagtatc
    13021 ttctataagt aagtcatttc atatcgatga aaacacgtta tttatttttg ttttatctat
    13081 aaaatggctg aaaaatgatt tttttctaaa tactatatat agtggcgtct atcctatata
    13141 ccactaaaaa actacaaaat ctagtctttt attttaaaaa aacacgtgta gaatgaagat
    13201 agacttttga gttagggagt gtttttttat gaaacttact gttttttgcg gttcccgttt
    13261 tggtaataaa gaaagttaca aatttattgc gcaaacgtta ggaaaataca tggcacaaga
    13321 agaaatcgaa ctggtttacg gtggttcata ctccgggatt atgggagtga ttagtaaaac
    13381 agtactggaa aataacggaa aagtcacagg tatctatccg aatgggcttt ttgaaactga
    13441 attgcctgga aaagatgtta ctacatttat tcctacagaa acgatcgatg aaagaaaggt
    13501 attattattt gaaaagggag atgctgtcct cgttttccct ggaggtctag gtacattgga
    13561 ggaattctca caacttcttt cttggatagc aatcggtctg acgcccgaca agccaatcgg
    13621 aatattgaat attggcggtt attacagcgg cttgcaacaa ttattagaaa cttttgcaaa
    13681 agaggatttt atggataaaa aatggttgga tcaagtaatc ttttccaaca atccattgga
    13741 actggtcaat gaattacgag cagtatctat taaaaatcag ttattattga aggaggcaaa
    13801 ataatgagtc aaatatattt agcaggacca tttttttcag aggagcaaat cgatcgagta
    13861 agtcgtatcg aaaaagcgtt ggaggaaaac aaaacagtca caagtttcta ctctccacgt
    13921 catcaccaag aaagcaatta tgaactattc agcgctggat gggcgcagga agtatacgaa
    13981 aaagatatgg aagaactgac aaatgcagaa tttgttgttg ctatccttga ctttgagcat
    14041 caaacaatcg atccaggaac ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg
    14101 atgatcattg tacaagaaga gacggtacct acgaacttga tgattacaca aagtctccat
    14161 acgtatttaa aatcagacca agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa
    14221 acttacgtag gcgagtactt atagaagagg aagggttgta tgtcagtgat tttgatttca
    14281 ggaacgattg gtgcaggtaa gagcagtttg acggatatgc ttgcaaaaga gattgattct
    14341 aaaccatttt atgaaaatgt cgaagataac gaagtattgc cgctctttta ttcaaatcct
    14401 gagcagtacg cttttttatt gcaaatcttc tttttgaaca agcgattttt agcaatgaaa
    14461 aatgctttag tgaacgatga caatgtgcta gaccgatcca tctatgaaga tagcctgttg
    14521 ttccatctaa acgctgattt aggtcgggta acggatatcg aagtacagca gtacgatagc
    14581 ttattagata cgatgctaaa cgagttagat gatgttgctc caaaaaaacg gcctgatttg
    14641 atggtccata tcaaagtttc tttggacacg atgctagaac gtatcaaaaa acgtgggcgt
    14701 gactatgaac agctagaaag cgatgagacg ttatatacct attatgaaac gttgaacacc
    14761 cgctataatc aatggtatga agattttgat gtatgtccta aaatacaagt ggatggggat
    14821 aaattcgatt ttgtagaaaa tccagaagat gcaaaaaagg tgatccgtca gatccaagaa
    14881 aaattagctg aattggaagg aaagcctgct ggaaaatatt ttacggataa ccagcatgca
    14941 aaaggtcaag aattaggcta aaaataaaaa accaaacgac gggaaagaaa aatt
//
LOCUS       DAYYAR010000065        14925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000065 DAYYAR010000000
VERSION     DAYYAR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.065"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..286)
                     /locus_tag="ACHAT0_001695"
     CDS             complement(89..286)
                     /locus_tag="ACHAT0_001695"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8451634.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(432..1310)
                     /locus_tag="ACHAT0_001696"
     CDS             complement(432..1310)
                     /locus_tag="ACHAT0_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8451635.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1349..2467)
                     /locus_tag="ACHAT0_001697"
     CDS             complement(1349..2467)
                     /locus_tag="ACHAT0_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8451636.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2464..4563)
                     /locus_tag="ACHAT0_001698"
     CDS             complement(2464..4563)
                     /locus_tag="ACHAT0_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8451637.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4614..5435)
                     /locus_tag="ACHAT0_001699"
     CDS             complement(4614..5435)
                     /locus_tag="ACHAT0_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8451638.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5432..6241)
                     /locus_tag="ACHAT0_001700"
     CDS             complement(5432..6241)
                     /locus_tag="ACHAT0_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8451639.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6262..6756)
                     /locus_tag="ACHAT0_001701"
     CDS             complement(6262..6756)
                     /locus_tag="ACHAT0_001701"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8451640.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6768..7268)
                     /locus_tag="ACHAT0_001702"
     CDS             complement(6768..7268)
                     /locus_tag="ACHAT0_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8451641.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7333..10029)
                     /locus_tag="ACHAT0_001703"
     CDS             complement(7333..10029)
                     /locus_tag="ACHAT0_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8451642.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10457..11530)
                     /locus_tag="ACHAT0_001704"
     CDS             complement(10457..11530)
                     /locus_tag="ACHAT0_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8451643.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11527..12354)
                     /locus_tag="ACHAT0_001705"
     CDS             complement(11527..12354)
                     /locus_tag="ACHAT0_001705"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8451644.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12355..13161)
                     /locus_tag="ACHAT0_001706"
     CDS             complement(12355..13161)
                     /locus_tag="ACHAT0_001706"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8451645.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13173..14258)
                     /locus_tag="ACHAT0_001707"
     CDS             complement(13173..14258)
                     /locus_tag="ACHAT0_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8451646.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14275..14817)
                     /locus_tag="ACHAT0_001708"
     CDS             complement(14275..14817)
                     /locus_tag="ACHAT0_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8451647.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
BASE COUNT         4542 a         3302 c         2441 g         4640 t
ORIGIN      
        1 aactttcttt ttttttcttg ctatcttttt ataatcacaa ctctctataa tagaattacg
       61 atttatttta tacggctgaa aaaatctatc accaaaaaaa ttcaaacatt aagccaagga
      121 ttatgaagtc aacatttcga aaaatgaaca tacattggta agcaactata actattacca
      181 cagacataaa gtaacacaat atattactat ttaagtgtac gccaccagtg aatatcctca
      241 aggtatacag cgcacaacaa aaagaaaaaa ggaaacgcag aagcatttcc tttcagtgat
      301 ttaatgaaaa caacacgatt tgacttagag cctttttatt tgaattcatt ttcgctttat
      361 ccaacttcat ttgatcattt gattactcgg ttaaatactt ttgacatgag tatcaatgaa
      421 acattcatta attatggttg cttatttctt accgcttttc tggccaatgc taatccgccg
      481 attattccgg cattatcacc taattcgggc gtaataatgt agctatcaat tggtggtatt
      541 tcaacatatc catttataag ctcaacaaat ttatcctgaa cttttttctt catgtgttct
      601 tgtttcatta caccaccgcc aaaaataatt ctatctggcg aaaacatcag tgttgtattg
      661 tatgcacatt gggcaaggta aaaggcttca atctgccaaa acgaatcgtc agccaataaa
      721 ttctggcctt ttacacccaa ccgtttttca atcgctggtc ctgctgccat cccctctaaa
      781 caatcttggt ggaagggaca gtttcctgaa aaattgtcct ttggatgtcg cttgatcaac
      841 atatgcccca tttccggatg actgaaccct tccacgaaag tcccattgaa caaggcacca
      901 cctccgatac ctgttcccac agtgaagtat gcaacacctg ctaacccttt gccagaacca
      961 gcaacatatt caccatagca agccgcattg acatctgttg tccattcaat cggaatattg
     1021 aaatattgct ttagcgttcc tacaaaattg aaattttgcc aagcaagttt tggtgttgat
     1081 gtgatatgac cgaaactcgt tgatttgcat tgaatatcaa ttggaccaaa gcagccgacc
     1141 ccgatgcctg ccaattgttc cttaaaaggt ttaaaaaaat caatgacctg cttcattgtt
     1201 tcaactggag ttgttgtggg aaaactgact ctttccaaaa tcgttaattc ctcagtaccg
     1261 actgcacaga caaatttagt ccctccagct tcaattgccc catatatcat cataggttcc
     1321 tcccttcgaa tatgtgaaaa acacttcatt aacgcggcaa gcgttctcga tcagtgaaat
     1381 gacgtttagt atttcctgaa gcttctgttg aataccagta agcgacacta gcaacatcgt
     1441 cttgccgctc aaataagcga acatcatcgt tgccgatttg ttgtaaagtg accgataaat
     1501 cttcggtaaa acagatgggg tcttgaagat gccaacgata aagaccatgt cgtggcattg
     1561 cgtcgtcacc aaaggcatga acggcattca attttcccgt ttcaaaacga tctcttgtgg
     1621 catctctatt tgattggtaa ggatacccta aaaaggctgt tgaatagttt ttcgcatgtg
     1681 gacgtccgaa ctcatcgtac tcatgaaagg cccacgcccc gccaaaataa tcttctgaac
     1741 ctgttgaggt tacggtcgga tactcactat ccccatcaat gtagaattta aactcgcctt
     1801 cgccccacca ataacgttct aggcaggtaa gcgcaaggta agtacccacg taatagcctt
     1861 gaccctttaa tcgatcaatt aacttataat cctcctgtaa tttggtaatg cgttcccgat
     1921 gccaatttgc gtgaaaatat aggctatttt cttccaattc gtccaccaaa gcgtaattta
     1981 tcgtataaaa aaatgaggac atatccttcg gatgtaaatt ctcgatcgta attttagcgg
     2041 atgtttggaa aggcatttcg aaataggaat taaagccccc tgtaggatta accactatcg
     2101 gcaacgagtt cacctcgcaa cgctctccaa acccattaca aaagaaatcc cctaaaggac
     2161 tttcaacagc agggctatcc gacccatccc aataaattct taagattaaa tcacgtaaaa
     2221 caaaactgcc aaattctgtt cgatcacgaa tcgtcatcca catatgacga attattcccg
     2281 ttccagtaat atccgcaatc acagtttctt caccagaagt cagcggaatt gcgccacgac
     2341 ctttgcggct tggtcctagc tcgcttggca acatcgcagc tttacctttc tcgcctgttg
     2401 gattttcggg agtgattgct cgactctgga tttttttaaa ttgggtaata ttttttagta
     2461 tactcatctt tttctccttt gaatgtgaat cgtttttccc ttttctacgt gaagaatttt
     2521 tttcgtatca cttattttca atggcttttc tgacaaattt gaaatactca actcggattg
     2581 cgtacattca atcgaaatga aggcttgacc gaacttcaaa ttcttccaag aaaacgttaa
     2641 attatcaaca ggatcaattt gcaatactcc ttttttgaat ttcaatccga aatattgact
     2701 gatcattagg ctgacaaaaa gacccgatgc ccagccgcat ttgccgcagc caaagtctcg
     2761 cacgacttct ccttgttgag cctctaagcg atacggccac caccaccacg aaccgtctaa
     2821 atctgccaaa caaattagtt cttctaatcg ggttacgaaa gcttgagggt tgtgcagctc
     2881 gctcattaaa acagtgataa agccaggaaa agtggcgccg gattctaatc cccaagtgat
     2941 gcctttgatt tcctcgctat aggttggatt gaaagaacta gcagaaaagg ccatggtgtt
     3001 ctgatataaa tgatctttat tatctaaaaa ttgataaaaa ggcatcaacg tagtgtctga
     3061 ttcttctcca tcatgcatca ataaattaat ttttccatcc acaatcacat cacttaaaaa
     3121 gattaaccct tgctcaagaa tcggtttttg atagtgcttc actgaatagg ttgttttctc
     3181 ctcatcaccg attccctcca aaaattgctc accaaaagga ccatcgatag tcatttgttt
     3241 acaaattgat tcctttagtt tacaggcaat gtttttgtat cgcttgctaa gactcagttg
     3301 ttctagggct tcaaaaattt ctgcaagtcc aatacaggcc ttccaaagac aaagattgct
     3361 gccggtatga tactttccaa gtgcaaaggc gtctgataac caaacagatg gaaaaagcat
     3421 tggttcccct gaccgacgtt gagctaatat gtcctcaata attcttgaag cattcagtag
     3481 aacctgcgga caccgctcaa agaattttgt atccccagta tgttcaaaat aaagactagc
     3541 cagtaacgca ctggataacg aattactcag agaatgattg atacctcctg taaattttga
     3601 accaggaaac ttaacgccat attgatcaaa ccatagaacc gttttttgac ataactccgg
     3661 ttcaaacatg gtgaatggca atgatgcgta atacatatcc ttattccaaa ttcgattagt
     3721 agcaggatag ctgccccaat tgctgccgac gacctgtccc tgtcgattca taccgaaact
     3781 agaaaagctt tgataaagtg cacgacgata gagatggaca actctatcgt ttggcattga
     3841 cagctgccca aatagctgag caaaatagtt caatgtttcc ttcagccatt tttcgatatc
     3901 agattcctga aaagtcatta cttcttcaaa agcatttggg tccacaaaag caatggcgaa
     3961 gcttgccact tcctgttgtg cgatcgtgtc cttatttcct ttttgagaaa tcaacacatt
     4021 ttgctgatcc gaatacttct cttgatacga aggcacatct ttcactgaaa cttgaaggct
     4081 ttcgtgtgtt tcattcacaa tgtaaacctg ttgaacaagc atacttaaac gcttattagc
     4141 cagtatggga gcaaaggata ctacaatgac cttaaacgtc gaataatgat gaatatatct
     4201 aggcaaacag tcttgaatca catcgacttc aatacgatga tccgattctg acaaatggta
     4261 gacaatcccg tttacttcaa cctttaagga taacgaattg gtttgagaga gctgtttatt
     4321 gacccaaaca cctggttttt cctcagttag taaattttct cgataaaagg tcaacgtttg
     4381 aatcgtcccg caaacatcaa ggtttgctaa tgtcttttga ttacttacat cgaaggtcaa
     4441 tccctttaca tcaggaatat tccgatagta gctgtattcg tttgaagtag tcacatttcc
     4501 gtcgtttaac tcagcattaa aaaatgggtg tttgggtgaa taaattactt cattaatagt
     4561 catcttttcc ctccttgcta aaaaaactgc agcctagtag ctgcagcttc ctactatgcg
     4621 attccaagta atgacaataa aattcccaag acgaccattc caaaaataat tttgatgacg
     4681 ccgacttttt tatctagtaa tttgaaacag ctcaatgtta ccaataacgg tacaattcct
     4741 ttaaaaatac tatcgaggat ttcttggaca ttcattagtt cttcgccctt cattgaaaag
     4801 ctcaaaacgg tattgaattt gaccatactg atcgtcattg cacctaccat gatcagaccc
     4861 aagatactag cagcttttgt cagaatttca attaacccgt tcgcatacag cttttcaata
     4921 tatttagagc ctaatgaata gcctaaaacg ccgccatagt atcgcatcaa gtaattaggt
     4981 atattgaaca taactaaaaa gataattggt gccaaaatat ttccggcatt ggctaagttg
     5041 atcgctagtc ccgcggtaac gacacgccaa actccccaga acaatgaatc gccaattcca
     5101 gccaatggtc ccatcaaact cgttttgatc gcgttgatac tttccacgtc aaaaccagct
     5161 ttttcagaat tttccttctc catcgatgcc gcaatcccca tgacaaagga cgcgcaagga
     5221 atggtcgtcg aaaaatactg tgtatgacgt tccagcgccg cagctcgatc ttcttcactt
     5281 ttgtagaaat gattaatgaa gggcatcata gaataggcga aaccaccgcc gcccatcgtc
     5341 accggattca ccgaagcata catagtcatc gagcgccaaa acatcttccg aaccaatttt
     5401 ttctcatctt tagaaattcc atcagctaat ttcattgaaa gaaatcctcc tcttcttggt
     5461 caagcgttgc atttacagca gcatcgcttt gaactgcatg aacagatata ttttttagtt
     5521 gtagatctct ttgggctata acaattgcaa taatcgttcc aactgcggca attgcaatca
     5581 atggtaactc gagatacgcc ataacaacgg ccccaaagaa aaaccacaca gcaatatttt
     5641 tactccaaag catccgtaac agcatcgcca tcccaactgc cggcagcaga tttccagcga
     5701 ctgttaatcc gttcatgatg acctctggaa tattatccaa gacgcttttg acaacatctg
     5761 ctccaaataa aacggcaaag aatggaataa gagcataaag cgcatagtgg acaaaccaca
     5821 taaagaagtg cagattgatg attccttttt gatcaccaga ttttgcaaat ttgaaaaatt
     5881 tagcagccat cggtccacat acaaaagaaa aaatcatatt cattaactgt aatcctaata
     5941 ctgctagtgg aaagacaatg ggaataatcg catccacgtt tcctcccatt ttgattgcga
     6001 acgctgtagc cagcccagca gccaaagaag gctccgccgt actagagatt ccgatattga
     6061 cgacacccat aaagatggct tctagtgacg ccccgacaat caaccccgtc tgcaaatccc
     6121 ccatcaacgc tcctgctaac ggagcaacaa caatcggacg actcagcatg gacatcccga
     6181 acatttcatg tccagcaact ccaataaaaa cggccaaagc gactacaaat gcatcataca
     6241 taatttttct ccttcctttt tctaaaaatc actcaaaata gagatagctg aacgtttttc
     6301 catcgccggt gtaggctgaa aaacggtatc tggataaagt tcaactaatt cttttaagtc
     6361 ttgaatttct gacgtcgtta acatcacttc ctttgataag gttttcttat cttccgatgg
     6421 cggtgtcatt ttcccagcat tccccacgtt cattaattcg atttccgttg ggaaagcttt
     6481 cgcaactttt accgcatctt tgacattagg gactaaaacc atcaagcgca tgtccttcgc
     6541 ccgcggatct tttaacaaat gaatcgcgtc atcaactgtt ttaataatga ctttgacatt
     6601 cgatggtgca gccatcttca atgtcatctt ttgaatatcg ttggtagccg cctcatcgct
     6661 tgctactact aacccggtta aatttagact ctttgtccaa gtcatcgcta cttgtccatg
     6721 aattaatcgt tcatcaattc gcactgcttt aatcattttt cttcctccta aaaaaaatct
     6781 tcctcttcat ttactacatg tacctcatca agaatggtca tctttaattc ctgttggcat
     6841 ttatttacca gttcctgaat atcctccttt gttaattcct tggttgccaa tactatttcg
     6901 agcagaattg gcagattaaa gccagacaaa atagttacag caatctcttt ctccaaaaag
     6961 gttttggtaa tcttctgatt gacacttccg ccaaataaat cggtaaaaac aaaaatctga
     7021 tcctgctcgt tcacctggtt tacaaataca ttcaactcct gttcaaaatc acactcatcc
     7081 acataagcat taatcaccgt aatctcttga accttccccg tcagtaattg aatactgcta
     7141 agaaagcctg aagctaagtg accgtgagtc gctattagaa ttttcttcat atggttaatt
     7201 cctttctttc tgttcacttt tatataagca attcctgtgc caacttttaa aaacattggt
     7261 ttaatcacgt taattcacat taaaaaaagt gtctaaccta aaaaaaacag attagacact
     7321 tttttagaca ctttattttt gaatatacgg tcctaataat tcatagagca gcgaaagctc
     7381 ataagcattg actttaaaat tatatttcat ctccattgat tgaaaaatac ttctagaaac
     7441 ataataaaat tcatcttcat ctggtttcaa atcataattc ggttcttcag atttttctgt
     7501 ccgagccaag aataaacgtt ccatcatcaa tgccgtatgc atatagagat ttagttttac
     7561 acggccattt aaagtgaaat gatagtaatt ttcatattta gaaatgactg tttccacttc
     7621 actcatcacg atcgttgggt tcagaaagtt caaacggtcc gcaattcctt ccaacgaaaa
     7681 gaattttact agctcctgaa tcaagcggtt aaattctgcc ggcttcattt caggactcaa
     7741 aatatgctcc aaattggctg ctccatcacc gtctaagatg tcataaacat taatattagg
     7801 aattgaaaag gaacgtggaa gatccgaagt cgtgatgacc aattttgtct tgtcaaaata
     7861 agtttcatca ttttggtcga ttaattcacg tagttctctg tagtccttcg tgaaaacttc
     7921 gatggattca tccgataaat gatgacgaaa aacatctttc aatttgtccg aaatccccaa
     7981 acccgacata caggaaatga taatgttgct actttgagaa aagccttcaa agtattgaac
     8041 attgattgaa tacttgtctg ctgcatcctc cgcaatctgt ttaaatggcg cattcatcgt
     8101 catcttcaag ccaatatcaa gtgcaatgga agtcgtcaaa ttattcagaa gaagtaactc
     8161 cccttttaaa tcgttcttga tctgtgtgta aatttgattt aaagagccca tatctacgat
     8221 cagcactaga ctatctgtat tcacttgccg tttcacgaaa ttccttgtcg attcgacaat
     8281 ctctgacaca ttactctcta ctggcatatc caacgcttca aaaacataat tgccgcataa
     8341 ttgattcact actgcttgaa tgctgctagc cgtactatct ccgtgagcaa gcaacaggcc
     8401 cttcagctcg atggattcat ctacataatc agataaagtc acgacaataa gcagtcttag
     8461 aaagttctcg tccttttctg agagcggtgg taaatgctcc aagaactgat tacataagta
     8521 agtcgtccga ggtagatttg cagctaatgt acgatttatc ccggttaagt ctgtcttata
     8581 gcttggggaa atgacatagg atctgctaat aatttctgca acaggtccac tcgtcgtcaa
     8641 tccgtacgtt tttcctaaaa cggataccca gacctttcta aacatctgaa tcatcaattt
     8701 tctactcatt gataaaaaag aatccttatc aatgtgatgc accaaatgct gtacttcatt
     8761 ctttatcgaa gaaaaatttt cattgaaatt accttcattt atcagctgag taattttttc
     8821 caaaatcgaa tcataatcaa acggtttgat tcgatccgtt tttatctttt gatttttctt
     8881 tatcaataaa ctagcaatcg gcaagctttc tacttcggat tccagcatat ttaactgaat
     8941 caagatctca tttttcccaa tcgttaatgg ttcttcttgt gttcgactat acacatccgc
     9001 acaactgatt ttaatgatat tcttcagctt accaatattc ccactaaagg gatgagataa
     9061 cattaaagac aaggcttctc ttgaaacaat aatatctttt tgtaaaacat ccgcttcatt
     9121 ttgaaaaaat aattgaatca gctctaatcg ttctcgaatg gggcgatctt caaaagttgg
     9181 caacataaca ctaatttgaa tacgccgatc aaaggtgtct aataaataat tttcaccttt
     9241 ttccgtcgtg gcgaaaataa aacgaacctt agcagaatgc caatttacat tttcaccgac
     9301 tggacgatag tttccagtat caataaatag aaagagcttt tcttgatttt ctttcgatag
     9361 gcgatggacc tcatcgagaa aaagatagcc gttgttggcc gttgccaata aaccatcggt
     9421 atccttttca gcccccgtaa aggccccctt cttgtatcca aacagtgttg ccgaaagcag
     9481 ttcgggattg tccgcataat cggcacagtt taagatagca aatggtgcat ctttttcaat
     9541 aatttgctca tggatagcgt attcaaaaat ttttgtggct aaaaaacttt ttccaacacc
     9601 cgtggctcca tttattaata cactgagtcc gttaggcgga tatttgacag cggctataca
     9661 tttttgaatg acctctctca gacttccgtc atgaccaact acagaatgaa aggcttcgtt
     9721 tatttgctcc gtattatcta caaccgcata ttcatctaaa ggcttccaca aaacaggctt
     9781 tcccggtaat ttctcgacct ttttatcttc cactaatctc gtcaaataat ggctggcatt
     9841 ctgacgagag atacctagat tcttcgaaat tttctcggca gtgacagaag acatattccc
     9901 tgttagtcgt atcagctcat caaaaactcg atctttagcg ctctttttca ttttttcctc
     9961 cagtttaatg tgtctaaaag cataataaac tatcggctgc aatatgtcta ttctatattt
    10021 gtattccaat ttttactttg aaacacaaat agtcgaatat ttgcctcaga tatctgacaa
    10081 aataatttat ctaccaattt agtttttatc taatatgatt tttatgtttt ttcatagcta
    10141 caaatattga actggattca tatattagtt ttattaatag attacagaag ggttaagtat
    10201 atttgcatag tttccaatta ctttatcctt ctgtaacttt ttcaatctta gatttactag
    10261 cccgtctcgg gccctatttt acaatggttt tgtccacttt tctctggtag aaaaacgtgg
    10321 tagaaaaaat aaaataacac aaaaaaagcg gcgtttcatc cgcttttggt agaagattgg
    10381 aaaactcaga tagaaaaagg acgattttta gctctttaga acgttgataa gtccccattc
    10441 ttactgagtt ttgccattat ctcctataca tcttgaattc taagaatagg tcattataga
    10501 tgcctaggta tttcgatcct agatctttgt agacttcaag atgggagatc gtgtcatcag
    10561 atggatagaa ttgttcgtcc gacgaaatgt cttttggcaa taacgctttt gcttcttcat
    10621 ttggtgtaga ataaccaata tactctgcat tttgtgccgc attttccggt tctagcataa
    10681 agttgatgaa atcataggca ccttctttat ttttcacggt ttttggcata acgatattat
    10741 caaaccaaag attggatcct tctggtggga tcacataatg taaatgttca ttttcccaca
    10801 tcatatcggc agcttcgcca gaaaatgtca ctgccacgga ggcttcttca ttgatcatat
    10861 acattttgat ctcatctgcc acgattgctt tgacatttgg tgttaaatga ttcaatttat
    10921 ttgctgcttc tgtcagctgt gccatattct tactattcaa agaatagcct aaactattca
    10981 aagaaagccc cataacttct cgtgcaccgt caatcagcat caaactgtct tttaattccg
    11041 gcttccacaa atcatcccaa tgcttaattt gatttgcatc aaagaattta tcgttatata
    11101 cgatccctaa agttccccaa aaatatggaa tcgaatactg attatctgga tcaaaatcca
    11161 agtctaaaaa tcgtggatca atattattta atcctttgat cttcgaatga tctaatggaa
    11221 gtactagttt ttctttgatc attttttgga tcatatattc acttggtata gcgatgtcgt
    11281 agtttgtccc accttgctgg atttttgtga acatcgcttc attagaatca aatgtttcat
    11341 aattcacttt gtatccatat tctttttcaa atttcgtgat caaactagga tcgatataat
    11401 ctccccagtt atagatggta agtacctttg ctccactcat gccacttgtt ttttcaagtt
    11461 gacgaacacc aagcagcagc accgcaataa tgatgagaat acctaaaaac agagattgta
    11521 attttttcat cgcagccgtg ccaccccctc gcgtactctt ctgtttttct tcagccgttt
    11581 tggacggttt tctttactaa taaagtagta tccaatgacc aatatcatgg agaacaagaa
    11641 aactaacgca cttagcgcat tgatttctaa actgattcct tgtcttgcac gagaatagat
    11701 ctctactgag agcgtcgtaa agccatttcc tgtgacgaaa aatgtaaccg caaaatcatc
    11761 aagagaatag gtaaatgcca taaaatatcc agctatgatt cctggagaaa gataaggcaa
    11821 aatgatattt ttgactactt ggaaattatt agcaccaagg tctctagccg catcgaccat
    11881 tgaatcgttc atttcctgca attttggcaa gaccatcaag acgacgattg gaatgctgaa
    11941 agcaatatgg gaaagcaata cactctgaaa acctaatccg aatcctagta gtgtaaagaa
    12001 aatcaagaaa ctagcaccga taataacatc aggagagact aataagatgt tattcatgct
    12061 caacaacgct gtccgtgcac gtcgtttttt cgtgtaatag attcccatag ccccaaatgt
    12121 cccgatgatc gtggcgatca atgcagataa gaaagccagc atgaacgtat tcaatacgat
    12181 aatgatcaaa cgtgtatctt caaaaacagc ttcataattt tcccaagtaa atcctgtaaa
    12241 gctgttcatc gttcctcctt cattgaagga ataaaaaatc agatagaaga tcggtgcata
    12301 taagaataaa aagacgatcc ctaaataaag atacgaccat ttaaattttt tcatttatga
    12361 gcgacctcct tttttcttct cgcctgtcag gagcatcacg atgaacatag caatgattaa
    12421 aatcacaccg attgtagaac ccatccccca gttttgtgta accaagaaat gctgttcgat
    12481 agccgttcct aaagtgatca cccgatttcc gccaatcaga cgagtcagca tgaataaaga
    12541 aagagaagga ataaatactg cttggacacc acttttcaca ccatttaatg ataacggaaa
    12601 aatcactcgt cggaaggtct caaagctatt cgcgcctaaa tctctactag cactgatcaa
    12661 agaaggattc atctcttcca atgcattgaa aattggcata atcataaatg gaatctcgat
    12721 atacgttgcc acgaacagaa aactgaagtc tgtgaataat atctgtttcg gcccgatccc
    12781 gataaaatca aagaaatgat tcacgctgcc gtgcacactg aaaatcccaa taaatgcgta
    12841 agctttaagc agcagattca cccatgtagg caaaataatc agcatcagcc ataattgctt
    12901 gtgcttcagt tttgttaaaa aataagcagt cgggtaactg atgatcagcg tgaaaaacgt
    12961 gatcaaaaat gcataccata cagaattgat cgtcatagaa agcgatgtcc ccgacgtaaa
    13021 ataagataga taattattca gcgtgaattg cccattcata tcaaaaaaag attgataaat
    13081 gatcattaat acaggcgcga tcacgaataa accgagccac aaaatataag ggacggaata
    13141 gatcctgcgc attgttttca ttcttgtccc ccctattctt cgtagctgtc aagacgtgca
    13201 tcgaattctt cttcagactc gttgaaacgc atgacatgga tatcttctgg ctcaaaggcc
    13261 aatcctactt tgcttccttc tttagctttt ttcgttgaat ggaccatcca ttcattttgc
    13321 tgttcatcat aacagatgat ttcataatgg acaccacgaa acagctgagt atcgaccgtt
    13381 acgactagtt ttcctttatc tggtgtcgtg atcgtcaaat cctctgggcg taaaacaact
    13441 tcaactggtt catttagacg catcccgcca tcgacacatt caaattgctt accaacaaac
    13501 tcaactagat tatcttcgat cattacaccg tccacgatat tgctctcccc aacaaaatca
    13561 gcaacgaaat gattgattgg ctcatcataa atatctacgg gtgtaccact ttggacgatt
    13621 ttccctttat tcatgacaaa aatctcatca ctcattgcca acgcttcttc ttggtcatga
    13681 gtaacaaaaa taaacgtgat tcctaatcgt tgttgcagat cccgcagttc atactgcata
    13741 tctgtacgta gtttcaaatc aagcgccgat aacggttcat ctaatagcaa tactttcggt
    13801 tcatttacaa ttgctcgtgc aatcgctaca cgctgtcttt gaccaccaga catttcacta
    13861 atctcccgtt tttcgtatcc aggaagctgg accatacgca aagcatcttt taccttcttt
    13921 tcgatatctg atttggacat tttcttgatc ttcaatccaa aagcaacgtt atcaaaaaca
    13981 ttcatatgag gaaaaagtgc ataatcttgg aagactgtat ttacttgtcg cttattagct
    14041 ggcaaatcat tcaaccgttt cccttcaaaa tagatgtccc cttctgtagc ttcggtgaat
    14101 ccagctataa tacgtaggat cgttgtttta ccacagccgg aaggaccaag cagtgtataa
    14161 aattttcctt gttcgatctc aaaacttact ttttttaata ctgtttcatc atcatagcgt
    14221 ttcactactt gatcaaatga aatgattggt ttccccacgt ttttaccccc tatattacaa
    14281 atatgattgc gttgcaacaa tcaatacttt tgttcgtttt ttgagatgat tccttaattg
    14341 atgttgtttt gtcgcttgat aatagatcga ctgtcctgct tttgccacat agaccgtctc
    14401 tcctaagctt aactctaatt ccccttctaa tacaaaaata aacgtttcag ataatgaagg
    14461 ttcaaacgtc ttatattctc ctgcaccttc aaaagtcagc aagacaggct ccatttcttt
    14521 ttcattcgaa tcagccacga gccattccaa ttcatacccg ttttcttcat cgaaataaac
    14581 cgtatgatct tcttttgaat aaaccacttg cagattcgct gcttcttgat gaaaaaattc
    14641 ttctggagtc acacctaaga cttcaagaat agaaaagaat gtttccattg atggtgaact
    14701 taaatctcgt tccaactggg aaatataccc ttttgacaaa tctgtgcgtt cgcctagttc
    14761 ttcttgtgtc aaattctttt ggatgcgcaa atttcttaac ttctcaccaa tttccataat
    14821 ttcctccgaa tcgaagtttt ctaatagtaa acttcctgtt tactaccact cgtattagta
    14881 tacgaaattt attgaaaaag gcaacccttt gtaaaatttt ttttg
//
LOCUS       DAYYAR010000066        14870 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000066 DAYYAR010000000
VERSION     DAYYAR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.066"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(208..774)
                     /locus_tag="ACHAT0_001709"
     CDS             complement(208..774)
                     /locus_tag="ACHAT0_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8451648.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(774..1730)
                     /locus_tag="ACHAT0_001710"
     CDS             complement(774..1730)
                     /locus_tag="ACHAT0_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8451649.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1730..2386)
                     /locus_tag="ACHAT0_001711"
     CDS             complement(1730..2386)
                     /locus_tag="ACHAT0_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8451650.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2756..4774
                     /locus_tag="ACHAT0_001712"
     CDS             2756..4774
                     /locus_tag="ACHAT0_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8451651.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4844..5050
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5201..7615
                     /gene="leuS"
                     /locus_tag="ACHAT0_001713"
     CDS             5201..7615
                     /gene="leuS"
                     /locus_tag="ACHAT0_001713"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8451652.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7963..9516
                     /locus_tag="ACHAT0_001714"
     CDS             7963..9516
                     /locus_tag="ACHAT0_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8451653.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9897..11681)
                     /locus_tag="ACHAT0_001715"
     CDS             complement(9897..11681)
                     /locus_tag="ACHAT0_001715"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8451654.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11696..13417)
                     /locus_tag="ACHAT0_001716"
     CDS             complement(11696..13417)
                     /locus_tag="ACHAT0_001716"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8451655.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13517..14278
                     /locus_tag="ACHAT0_001717"
     CDS             13517..14278
                     /locus_tag="ACHAT0_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8451656.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4838 a         2734 c         2946 g         4352 t
ORIGIN      
        1 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
       61 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      121 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      181 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      241 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      301 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      361 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      421 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      481 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
      541 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
      601 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
      661 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
      721 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
      781 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
      841 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
      901 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
      961 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1021 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1081 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1141 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1201 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1261 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1321 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1381 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1441 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1501 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     1561 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     1621 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     1681 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     1741 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     1801 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     1861 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     1921 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     1981 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2041 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2101 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2161 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2221 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2281 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2341 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2401 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2461 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     2521 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     2581 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     2641 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     2701 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     2761 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     2821 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     2881 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     2941 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3001 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3061 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3121 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3181 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3241 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3301 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3361 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3421 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3481 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     3541 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     3601 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     3661 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     3721 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     3781 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     3841 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     3901 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     3961 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4021 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4081 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4141 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4201 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4261 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4321 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4381 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4441 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4501 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     4561 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     4621 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     4681 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     4741 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     4801 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     4861 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     4921 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     4981 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5041 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5101 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5161 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5221 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5281 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5341 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5401 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5461 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     5521 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     5581 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     5641 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     5701 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     5761 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     5821 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     5881 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     5941 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6001 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6061 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6121 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6181 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6241 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6301 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6361 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6421 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6481 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     6541 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     6601 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     6661 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     6721 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     6781 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     6841 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     6901 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     6961 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7021 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7081 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7141 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7201 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7261 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7321 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7381 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7441 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7501 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     7561 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     7621 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     7681 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     7741 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     7801 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     7861 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     7921 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     7981 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8041 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8101 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8161 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8221 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8281 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8341 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8401 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8461 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     8521 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     8581 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     8641 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     8701 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     8761 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     8821 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     8881 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     8941 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9001 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9061 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9121 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9181 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9241 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9301 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9361 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9421 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9481 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
     9541 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
     9601 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
     9661 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
     9721 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
     9781 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
     9841 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
     9901 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
     9961 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10021 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10081 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10141 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10201 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10261 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10321 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10381 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10441 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10501 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    10561 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    10621 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    10681 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    10741 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    10801 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    10861 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    10921 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    10981 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11041 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11101 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11161 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11221 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11281 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11341 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11401 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11461 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    11521 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    11581 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    11641 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    11701 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    11761 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    11821 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    11881 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    11941 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12001 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12061 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12121 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12181 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12241 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12301 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12361 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12421 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12481 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    12541 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    12601 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    12661 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    12721 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    12781 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    12841 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    12901 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    12961 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13021 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13081 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13141 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13201 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13261 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13321 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13381 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13441 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13501 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    13561 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    13621 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    13681 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    13741 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    13801 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    13861 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    13921 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    13981 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14041 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14101 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14161 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14221 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14281 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14341 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14401 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14461 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    14521 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    14581 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    14641 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    14701 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    14761 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    14821 aaaaatcaca aaggttattg ttatttttat atattctttt taagaatgat
//
LOCUS       DAYYAR010000067        14815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000067 DAYYAR010000000
VERSION     DAYYAR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.067"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHAT0_001718"
     CDS             complement(296..1024)
                     /locus_tag="ACHAT0_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451657.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHAT0_001719"
     CDS             complement(1002..2576)
                     /locus_tag="ACHAT0_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8451658.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHAT0_001720"
     CDS             2805..3743
                     /locus_tag="ACHAT0_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8451659.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHAT0_001721"
     CDS             3755..4666
                     /locus_tag="ACHAT0_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8451660.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHAT0_001722"
     CDS             4690..6162
                     /locus_tag="ACHAT0_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8451661.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHAT0_001723"
     CDS             6213..6824
                     /locus_tag="ACHAT0_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8451662.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHAT0_001724"
     CDS             6854..8767
                     /locus_tag="ACHAT0_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM8451663.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHAT0_001725"
     CDS             8895..11876
                     /locus_tag="ACHAT0_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM8451664.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHAT0_001726"
     CDS             11873..13534
                     /locus_tag="ACHAT0_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM8451665.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHAT0_001727"
     CDS             13574..14506
                     /locus_tag="ACHAT0_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8451666.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAYYAR010000068        14446 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000068 DAYYAR010000000
VERSION     DAYYAR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.068"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..992
                     /gene="cdaA"
                     /locus_tag="ACHAT0_001728"
     CDS             105..992
                     /gene="cdaA"
                     /locus_tag="ACHAT0_001728"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8451667.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            989..2158
                     /locus_tag="ACHAT0_001729"
     CDS             989..2158
                     /locus_tag="ACHAT0_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8451668.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            2182..3537
                     /gene="glmM"
                     /locus_tag="ACHAT0_001730"
     CDS             2182..3537
                     /gene="glmM"
                     /locus_tag="ACHAT0_001730"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8451669.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(3609..5414)
                     /gene="glmS"
                     /locus_tag="ACHAT0_001731"
     CDS             complement(3609..5414)
                     /gene="glmS"
                     /locus_tag="ACHAT0_001731"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8451670.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            5824..6114
                     /locus_tag="ACHAT0_001732"
     CDS             5824..6114
                     /locus_tag="ACHAT0_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451671.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            6127..7152
                     /gene="sppA"
                     /locus_tag="ACHAT0_001733"
     CDS             6127..7152
                     /gene="sppA"
                     /locus_tag="ACHAT0_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8451672.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            7155..7814
                     /locus_tag="ACHAT0_001734"
     CDS             7155..7814
                     /locus_tag="ACHAT0_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8451673.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            7971..8903
                     /locus_tag="ACHAT0_001735"
     CDS             7971..8903
                     /locus_tag="ACHAT0_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451674.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            9057..10346
                     /gene="tig"
                     /locus_tag="ACHAT0_001736"
     CDS             9057..10346
                     /gene="tig"
                     /locus_tag="ACHAT0_001736"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8451675.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            10593..11843
                     /gene="clpX"
                     /locus_tag="ACHAT0_001737"
     CDS             10593..11843
                     /gene="clpX"
                     /locus_tag="ACHAT0_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8451676.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            11926..12513
                     /gene="yihA"
                     /locus_tag="ACHAT0_001738"
     CDS             11926..12513
                     /gene="yihA"
                     /locus_tag="ACHAT0_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8451677.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(12587..12742)
                     /locus_tag="ACHAT0_001739"
     CDS             complement(12587..12742)
                     /locus_tag="ACHAT0_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8451678.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            12878..14206
                     /locus_tag="ACHAT0_001740"
     CDS             12878..14206
                     /locus_tag="ACHAT0_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451679.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            14217..14426
                     /locus_tag="ACHAT0_001741"
     CDS             14217..14426
                     /locus_tag="ACHAT0_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451680.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         4770 a         2525 c         3097 g         4054 t
ORIGIN      
        1 aaaattatgc gtttttctca agaaaaatac gagcagatca gcctctaaaa ttaaaacata
       61 tgatatacta gagaggaaat ctcaaaagag aagagaggct ttttatgtca tttcaaatcg
      121 gggaactttt taatttggac tattggcagc aaatgttttc cggtgacttg ttctctctga
      181 attttttggt gaacatactt gatattctgg ttgtctggta tcttgtatac aaactgatcc
      241 aactgctgcg aggcacaaaa gcgattcagc ttctgaaagg tgtagctatt tttattgtta
      301 tccgaatcgt agcggaaatc attggtctgc acaccttgtc ttggctgatg aaccaagtca
      361 ttacgtacgg ggtgatagct gctatcgtga tttttcagcc ggaaatccgg agaggactgg
      421 aacatcttgg acgcagttcg cttttcaaac aatcaaaaag cgaacagcag gaagatgaga
      481 agatgatctt gtcttttgac aaagccatcc aatatatgtc caagcgaaaa atcggtgcgc
      541 taattactat cgaacggcat actggattag atgagtatat tgagacagga attgcattag
      601 atgcagatat cactggagag ctgctgatca atatctttat tcctaataca ccgctgcatg
      661 acggtgcagt tattgtaaaa gaaggaaaga tcgcagtagc aagcgcctat cttcctctat
      721 cagaaagtat gttgattcca aaagaattcg ggactcgaca ccgggcagct gtcggtataa
      781 gcgaagtcag cgacgcgatt accatcgttg tctctgaaga aactggggat gtcagcatta
      841 cattagataa tgaactgatg gctggcttat cccagcaaga atatttggca attttgcgta
      901 gagaactgat tgctgaagag aaaacaaaaa acaagaaaaa cgtgattcaa tctttcctag
      961 acggtatgac taagggaggg gatcgcaaat gatctcaaaa gagaaacgga caagcattct
     1021 ttacagtttt attgcattgc tgttcagtat tgcgctgtac ttcaatgcaa atggccagtc
     1081 cgttccaaat acgctttctg gaaatgaagc gtataatcaa acagttactg gggtgccaat
     1141 caagatttct tatgattcaa agcgctacta tatccatgga tatgaaaatg cagtgactgt
     1201 caaactttcc agtgcaaacc gagttcagtt gaatgcagag gcaaacgaag atacacggat
     1261 gttccgcgtc acagcagata tcactaagct gggcgaagga acacatgagg ttccgctaaa
     1321 aattcaaaat ttgagcagtg ctgtcacagc aaagatcgaa ccatcgacta ttacagtaac
     1381 ggttgagaaa aaggtaataa aagatttcga tgtggaaacg ttgattcctt caacgttcac
     1441 accatctgga tatgaacttg atgatacttc cgttagtcct aaaaaagtct ctatcactac
     1501 aggtgacaaa acattggcag aaatcaaacg agtagtagct gatgtggacg cttcgatggt
     1561 cacagatgat gggattaata gtgaggtccc aatacaggcg ctgaatgcag caggggaagt
     1621 attaagtatc gtctctgatc cagtgcaagt aacagtgaga gctgattcga tcaagccaag
     1681 caagtcggta cgactttatg ggatacagca aggaacacca gctgccgggg tagaaagcta
     1741 tgatttcagt ttttcaaaac tggaagcaga agtaagcggc tctagcgatt tgcttgcatc
     1801 gatcggtgac agtattgctg taccgatcaa tgtctcaggt atcaatcatc gaacaactcg
     1861 taaaattgaa ataccagtag aagaggggtt aagtatcaag cccgaatcag ttagtgtgga
     1921 gattacaccc gtgttgcaaa cgtcaagcag tagctctgct tcgacgagca gtcggacaca
     1981 agaaaataca agtacgagta gaagtatttc atctgctgat tcaggaacgg aaccatcgac
     2041 aacaaccatt tcttccagtt cggaatctgg gacaagcagc tcgaccacaa gtacagaaga
     2101 aacatcagaa acaagcgaat caacacaaat gagcagaaca aattcctcag aaaattaagt
     2161 taaaggagta attttacaac tatgggtaaa tattttggaa cagacggcgt acgcggtgaa
     2221 gccaacaagg aacttacgcc agaacttgca tttaagctgg gacggtgcgg aggttatgtc
     2281 ctaagccagc atgaagaaga aggaaaacgc ccacgcgtgt tagtagggcg ggatacaaga
     2341 atctcaggtc aaatgttaga agaagcgctg atttcaggac ttttatcggt gggtatcgaa
     2401 gtgttccagc taggtgtgat ctctacacca ggtgttgctt atctgacacg tttgcaaaaa
     2461 gcaagcgcag gggtcatgat ttctgcttct cataatccag cacaagataa tggaatcaaa
     2521 tttttcggtg cagatggttt taaactagta gatgaccaag agctagaaat tgaagcatta
     2581 ttagatgctg aagtggatga attgcctcgt ccatcagctg aaggattggg aactgtagaa
     2641 gaatttccag aaggcttatt gaaatattct caattccttg tacaaacgat caacggtgac
     2701 ctttccggtt tgaccatttg tgtcgatgca gcaaacggag caacttcaac aagtgttaat
     2761 cgtttgtttg ccgatcttga aacagatttt tataccatgg gcacaaatcc agacgggtta
     2821 aatatcaatg atggcgtagg ctctacccat ccagaacgtt tagctgagat ggtcgttgaa
     2881 aaaggagcag atgctggact agctttcgat ggcgatggcg atcgtattat tgcggtggat
     2941 gaacttggaa acattgtcga cggtgataaa atcatgttta tctgtgctaa atacttggca
     3001 gaaaaaaatc gattgaaaaa agatacgatc gttacgactg tcatgagtaa cttaggattc
     3061 cacaaagcgg tggaaggaat cggtttgaaa gatgtcgtta cacaagttgg tgatcgttac
     3121 gttgtagaag aaatgcgcaa aaacgattat aactttggcg gcgagcagtc tgggcacatg
     3181 attttcttag attttaacac aactggagat gggatgcttt caggaattca gttactgaat
     3241 atcatgaaac agacaggtaa gaaattatca gagcttgcag cagaggtaac aatctatccg
     3301 caaaaattag tgaatatccg agtaagtgat aaaaacggtg cgatggatgt tccggcaatc
     3361 aaaaaagtga ttgaagaggc agaagctgaa atgggatcag aaggaagaat cctagttcgt
     3421 ccttccggta cagaaccatt acttcgagtg atggcagaag cgcctactga tgagaaagtt
     3481 aattactatg tcgataaaat cgcggcagta gtcaaagaag aaatcggact ggattaagag
     3541 atattttcat aaaaaataaa agaacaagga ccagtgtcag atgtctgaca ctggtccttg
     3601 ttcttttatt attctactgt cactgatttc gctaaattac gtggtttgtc aacgtcgaat
     3661 ccccgtaata gcgttgcgta atatgcaagt aattgagtcg gtacgactgt ggttagagta
     3721 gttagataag ggtggacagt tggtagcacc agctggtcac tttccttttc taatccctct
     3781 gagacaataa ctagtgtttt agcgccacgg ctttcgactt ctttcaggtt tcctcgagta
     3841 tgcgcaccag tcacttcttc actgatgatt ccaatcactg gtgttccttc ttcgatcaaa
     3901 gcaatcgttc catgtttcaa ttcgccagcg gcaaaacctt ctgcctggat ataactgatt
     3961 tctttcaatt tcaaagcagc ttcaagagaa acgttgtagt cagcgccacg tccaatgtaa
     4021 aaggcatttc ttgtatcgct gagatatact tctgccaact cttctaaata atctttttgg
     4081 gcgatgaccg attccatcgc attagcaact aaactcaatt catgagtgat atcgaataca
     4141 agagaagttt caaattcttt tttatcaccg atagctttgg ctaataaagt tagtacagct
     4201 atttgtgcag tataagcttt cgttgatgct actgcgattt cgggtccagc atgcaatagc
     4261 agtgtaaagg atgcttcccg cgaaagagta gaaccagcaa catttgtaat cgtaagtgaa
     4321 gggtagccga gacggttgat ttttaccaga acttggcggc tatctgctgt ttcgccactt
     4381 tgactcaaga aaataaagaa tggttttttt gaaagaagag gtgtgttata gccaaattca
     4441 ctggctaaat gaacctcaac agggatctgt gtcaattttt ccagagtctg tttacctgct
     4501 aaccccgcat ggtaactagt tccgcaagca acgatataga ttcgatcgct atcagacaat
     4561 gtatcgacca gcttttgatc aagagagaca cgattgtctt ctgttacgta tttttgagcg
     4621 atacgacgca tgataatagg ctgttcgtcg atttctttca gcatataaaa aggataggta
     4681 cctttctcga tatctgaaga atctacttgt gcttcgaatg aagaacgttc tacgatgtca
     4741 cctgcaaaat tttgaataat aactttttct gatgtgacgg taaccatctc ttcatcttca
     4801 atctcaacga attccttcgt atgagcaagc atagccattg catcgctggc aatcacattg
     4861 aatccatcgc cgagtccgat taatagtgga cttttatttt tagcgacgta aataacatca
     4921 ggtgctgtcc gatcaatcaa agcgaaagcg taagaacctt tgatgacccg caaagctttt
     4981 aaaaacgcct cttgcgttgt agtatccttc gcgaaagttt caattagatg agcgataatc
     5041 tcagtatcgg tctcaccaat aaagtggtca tttgctaaat aagcaatttt aagttcttca
     5101 aagttttcga ttacgccatt gtgaacgagc acgaagcgtc cactttggga agtatgtgga
     5161 tgggcgtttt cttcagaagg ttgcccgtga gttgcccagc gtgtatgacc aatacctata
     5221 tttcctgtag tatcaatatt tattttgttt ttcaaatttt gaattctgcc ttgtgctttg
     5281 accagataat cagttgtgcc atctgagaca aagataccag ctgaatcata accacggtat
     5341 tctaattttt ctagaccgtc aatcaatcct ggtgttacat tttttaatcc aatcattcca
     5401 acaattccgc acataaaaaa catcctactt tctataatga attcgtatga atgcttccag
     5461 ctgcaggaaa atccttttgt tttatctttg cagatatttt ttgtggattt tctgtagagt
     5521 gcagtcccta gggccatccg ccgaatcttt cgataaaccc tctcctcgtc atctgtttac
     5581 acagacctgg cgctatccta aaaggtataa ctatcctcct cttttgtttt ttatctggaa
     5641 acatttaaaa tcataacctt ttgcgaatgt tcttgtcaac aaaaaaagaa taaaaaataa
     5701 attggtatat atcaaatgct ttgtcaaatg ctctttttat gtcaaaaaat aatgggtttt
     5761 tatctatcat ctttttcagg agttcggtac aatagaaagt aatcggatag aggaggagta
     5821 aaaatggatt caaaaattga gatcatgaca ctgggaatgt taaaaaaaca attgtccaaa
     5881 ttcgaagcat cagctggtgt atcagatgat acgaaaatat ttttagatac tggctgggat
     5941 agcatccaag aaatcgcacc tgatgccttg gaagttgctg aagctcgtga gtttacggta
     6001 gaagatgaat tgacaaaaga gtcgttttcc gggtatgcgc gggaagaaaa agcagagcgg
     6061 ttcgacactt ctgagcagtc agaaactgtg atcgtcataa aaaacttgta ttagaaagga
     6121 gaaagaatga ataagagacg ttgggcagca gttctgattg ctgcaggatt atttgtggtt
     6181 tcgttagttt cagctagttt gactaagcct caggaaaacg atcaaattga gggattgaac
     6241 agttggttat atggggatga agaactagct tcaaatgtgt tggaagaggg agacagtacg
     6301 caaaaaatcg taaaattatc agtagatgga acgattgctg cgggaggggc ttcaggttta
     6361 tttgcttctg aaggttacaa tcataaaaat tttatggaac aactgaaggc gattgaagaa
     6421 gatccaacag tcaaaggtat ctttttagaa gtaaattctc ctggtggcgg tgtctacgaa
     6481 agtgcggaaa tcgctaagaa attggatacg attcgtaaag aacatgatat cccaatgtat
     6541 gtcagcatga aaaacatggc agctagcggt ggctactata tttctgctca agcagataaa
     6601 atctttgcaa cagaagaaac agtgaccggc tcaattggtg tgatcatgtc aggattgaat
     6661 tattcaggac ttctagaaaa actgggtgtg gaagatacaa cagttaaaag cggagcgttg
     6721 aaagatatgg gttcaggaac gcgaccagag acaaaagaag aagaagcggt gcttcaagca
     6781 tatatcgaca gcgcatatag tcgttttgta gcaatcgtca gcgagggacg gaataaatct
     6841 gaagaagaag tcaaaaaaat tgctgatgga aggatttatg atggtgtcca agcaaaagaa
     6901 gtaggtttag tcgatgaatt gggatttcct gaagatgctt taaaagctat gcgaaaggaa
     6961 caacagcttg aagatgcgga actagtagaa tattcttcca cgccatctgg gtttgctagt
     7021 acatggttag gaagtaaact agcagaattc cagggattga agccttctga gactagtcag
     7081 atcttatccg ttcttgagaa tctagggaca gcggaatcac caaaagcaat gtattattac
     7141 ggaggtgagt aagcatgtca gagctttcaa atgaaaataa ggaacaagaa aaagataagc
     7201 aacagcagcc tgctgtagga cctgactcca acttttccca aaaggtactg agctcttttg
     7261 aaagagagcc tttgacgccc gaagaaatca aaaagaaaca acgtaaatgg aaaaagtata
     7321 cggacacaag aaatgaaccg ccaaaagcaa atgattttcc taagtatttc tatgcaggtt
     7381 tttttatccg aatgtttgct ttcttggtcg atcttttgtg tatcggagct attacgcgtt
     7441 taacacttgg tattgccaaa aatctaggtt ggcttaattt ttcagattct tatctgagta
     7501 tctacggatt cacagcctta cttatttatc tagcgtattt tattttattg acgaaattca
     7561 atcatggtca aacgattggg aaaatgatct ttggtattcg agtcatcagc tttgatgaag
     7621 aagaacttag ctggtcaacc gttttagtta gagaaggtgc ttgccggttc attctaaaat
     7681 ttcctttgct gatgttaggg tatcttccag caatctttaa tcagaaaaag caacatctag
     7741 gcgatttttt cactaataca agtgttgtta cgatcaatct gatcaaagca ttcaaccagg
     7801 aagtcaacag ctgatgagca ataaaaagca gtaacagttg aaagcacctt ctaaaagtca
     7861 gatctaaaat ctagctttta gagggtgctt cagttatttt taattgtcat gctttttttg
     7921 ttttgctact attgaacatg agctgaacat gagcaagagg aggacactgc gtgaagccga
     7981 ttgattttcc agaaaaatat gagaatttaa tgcgagtagc acaacaagca ctagcaaacc
     8041 agcaatatca gcaggcaaaa gaattatttc aacgagctta cgagttgaaa gaaagctttg
     8101 aggctaatag cctgcttgtt ttttgtcttt acgagttgga cgagaaaaaa gaagcattga
     8161 agcaagcact cttgcatgaa aaacagtatc ttcaaaatga ggagtttgct gaattttatt
     8221 ttgacttgtt aatcagtaca caagattttt tatatgctag aaaattgata gcaagtacag
     8281 atttttatga agctttcgag cagcgggtca ttgaaaaaat ccaatttgca gaagaattat
     8341 ccggacaaat ggagaggcag aagatcaagg cactccacaa taaatcggag gaacttcctt
     8401 cgttggaacc tgcacgtcag cttgcgtcta ttgatgagat cgaacagctt ccttatcatg
     8461 agtttatcca aacagcgagt aaacttattg tatcgccaga ggtccatatt ttagcaaggg
     8521 caaaattact ggagacactt aggcaattga acgaatgcca tcctgttttc tatttgacga
     8581 ttgaagaaaa gttagtcaaa gtcataccaa aagatctgcc aaagccgcag cagcaggcaa
     8641 gttaccagca attatgtgta ttttcagatc gttacggtaa tgaagatgcg ctgcttagtt
     8701 ccgtgttaaa tgaagaattc acgttgcaat ctgcaattgt ttatcctgtc tatgatacat
     8761 acatcgaaga cccgaagcgc tggtttcaac tgactgtaga ggcgtgtaca ggaaaaacga
     8821 tgtatgaagg tacagaagat gaaaaacaag attttttaaa aaaaagagaa aaaattttgc
     8881 agcaaatgat cttcttccat tagattttat cagtgaaaaa gataaaaatg ttatgaaaaa
     8941 caaggattct atccattcaa tatgtttaca atttgaaagg gttagtgtac tatttaaagg
     9001 tatgcatgaa acgtttttga tgtataaaat gaatttctaa actcggaggg aattaaatga
     9061 ctgcaacatt tgaaaaaaaa ggcacgaatg atggtgtttt aaaatttacg attgcgcaag
     9121 acgaaatcca aaaaggatta acaactgcgt ttaataaagt gaaaaaaagc ttgaacgtac
     9181 ctggattccg taaagggaaa gtttctcgtc aagtattcaa ccgtatgtac ggtgaagaag
     9241 ctttatatga agatgcattg aatgctgtat taccagaagc ttatgaagct gctgtcaaag
     9301 aagctggcat cgatcctgtt tctcaaccaa aaatcgatgt tgaaagcatg gaaaaaggtc
     9361 aagactgggt aatcactgct gaagtaacag tgaaaccaga agtaaaatta ggacaatata
     9421 aagatctagt tgttgaaaaa caagaccgtg aagtaacaga tgctgacgtg gatgcacgta
     9481 tcaaacgtga acaagaagca caagctgaat tagttatcaa agaagatgct gcagctgaaa
     9541 atggcgatac agtagtaatc gacttcgaag gctttgtaga tggcgaagcg tttgatggcg
     9601 gaaaaggcga aaattactca cttgaattag gttcaggctc attcattcca ggttttgaag
     9661 atcaattagt tggtcataaa tcaggtgaaa gtctagatgt aaatgtaact ttcccagaag
     9721 attatcaagc agaagatctt gctggaaaag aagcagtctt caaagtgaca atccatgaag
     9781 taaaagcaaa agaagtgcct gaattagatg acgaattcgc aaaagatgtt gatgacgaag
     9841 tatcaacatt agacgaattg aaagaaaaat atcgcaaaga attagtagaa acaaaagaaa
     9901 aagcagcgga cgatgcaaaa gatgaagcag cgatccgtat ggctgttgaa aacgcagaaa
     9961 tcgttgaatt gccacacgtg atggttcatg atgaagtaca tcgttcaatg gacgaattct
    10021 tgaacaacat gcaacgtcaa ggtatctctc ctgaaatgta ctatcaatta actggttcta
    10081 cagaagaaga cttgcataaa caattcgaag gtgaagcaga aatgcgcaca agaacaaatc
    10141 ttgtgatcga agcaattgca gctgctgaaa atcttcaagc aacagaagaa gaaatcaatg
    10201 cagaaatcca agaattatca gaaacataca acatgccaat cgaacaaatc aaacgcgtgt
    10261 tgacagaaga tatgctggaa catgatgtga caatgaaaaa agcaatcgaa ttgatcacag
    10321 gtacagcgac agaagcgaaa gaatagaaat caagtaataa atcatttctg aagaagctac
    10381 ttctagaaaa ttgtttattc gaaataaggg actgtgacac gattttgtca cagttcctta
    10441 ttttttgtta agagtaaaaa atgcaaaggt ataggtcagt tgatcctttg ttaaacactt
    10501 tttcttttta ctaaactatg ttatgatagt aaaagactgt aaagtctttg atagtttctt
    10561 gcatactttt tgcaagttaa ggggtgaaga ccatgtatga caatccaagt agcaatgaga
    10621 ccgtacgctg ttctttttgc gggaaaaccc aagaagaagt gaaaaagatc gtagctggtc
    10681 caggggtcta catctgtaat gaatgtatcg acctatgtaa agaaatcatt gatgaagaat
    10741 tttatgaaga agctgtacgc gaattcactg aagtccctaa accactagaa attcttgaag
    10801 tattgaataa ctacgtgatt ggacaagatc gcgcaaaacg ctcgttagct gttgccgtat
    10861 acaatcacta taaacgtgtc aatcagcaag cacaagaaaa cacagatgat gttgaactgc
    10921 aaaaaagcaa tatctgcttg atcggaccta cagggtctgg taaaacattt ttagcacaaa
    10981 cattagcaaa aacattgaat gtaccttttg caatcgcaga tgcgacaagt ttgacagaag
    11041 ctggttatgt gggggaagat gtagaaaaca ttttgcttaa attgcttcaa tctgctgatt
    11101 acaatgtgga acgtgcagaa aaagggatca tctatattga tgagatcgat aaaattgcgc
    11161 gtaaaagcga aaatgtatct attactcgtg acgtttccgg cgaaggtgtc caacaagcct
    11221 tgctgaaaat tctagaagga actgttgcaa gcgtgccacc tcagggagga cgtaaacatc
    11281 cacatcaaga atttattcag attgatacga cgaacatatt gttcatcgta ggtggtgcat
    11341 ttgatggcat cgaaacgatt gtgaagaatc gtatgggcga aaagacgatt ggatttggta
    11401 cacaaaacca aaaactttct gaagacgaaa gtgtcatgca gcatatcatt cctgaagatt
    11461 tactaaaatt cggattgatc cctgagttta tcggacgatt acctgttatg gcagctttag
    11521 agaaattgac aacagatgat ctggtacgga tcttgactga gccgaaaaat gcgctagtca
    11581 aacaatatca aaaacttctt tctctagata ataccgaact tgaattcgaa cctgaagcgt
    11641 tgagagccat tgcgaaaaaa gcaatcgaac gcaataccgg tgctcgagga cttcgttcca
    11701 tcattgaaga aatcatgatg gatgtgatgt ttgatatccc ttcaaatgaa aacatcgaaa
    11761 aagtgattat cacaaaagaa gcagctgaat tgtctggtga gccaacagta atctataaag
    11821 attcagagaa aaaagcgggt tgatgaaaag aggctgccgt aaaagtcatt ttggcttttg
    11881 cgacagccgc ttgctttatt catcaccttg ttaaggagag agaccatgaa agtccatcaa
    11941 gctgaaatta tcataagtgc agtggcacca aaacaatatc cagatacaca attgccagaa
    12001 atagctctgg caggacgatc aaatgtaggg aaatcttctt ttatcaacac attgattgat
    12061 cgaaagaatt tagcccgtac ttccggtaag ccaggaaaga cccagacatt gaatttttat
    12121 ttgatcgaga acgcattaca ttttgtcgat gttccgggat atgggtatgc aaaagtatcc
    12181 aaaactgagc gagcaaaatg gggaaagatg atcgagacat atttaacgac tagagaccag
    12241 ctacgtgcgg tcgtttcttt agtagatttg cgtcataagc cttctcaaga agatatccag
    12301 atgtatcaat ttttgaagta ttatgatatc cctgtaattg ttgttgccac taaggcagat
    12361 aagatccctc gtggtaaatg gaacaagcat gagagcgaaa tcaaaaaagc gttggatttt
    12421 gatcgcaatg acacgtttat tctcttttct tccgttacaa aacagggaaa agaagaagcg
    12481 tgggcagcaa tcgaacaagc aattgaatca taaaaagaat aaaaaagact taccagtcaa
    12541 actctttcat aaaagtttgg ctggtaagtc tttttttgag cttaacttac ttttgtttct
    12601 tgtctttttt cttttcattt tgtggctgtg gttttagaaa tcgatcgatc tcttcttttg
    12661 gtgcaactgt tttttccgga tcgacagcaa atgattcgaa aagtttatct aaattatctg
    12721 tacgttcaaa tttcaacccc attgtaaaca cctctttgtt tctttttact tagtctatca
    12781 tgtaatcgtc caaattggtt tctaagacat aaagttttag tttttcttta tcgttggtta
    12841 taattaaatt ataaaaagat tttaaagagg tgagaaaatg attggcgaag ataaattatt
    12901 gatgactatc cagctcacag acttaaatga agaagaaaaa aaacaggaaa taagcgattg
    12961 ggctattttg acacgtctgt tgatccagcc aacatttatc cgctctttca cacaacaagc
    13021 ggatccgtat gatcttcgaa agcttcaaga aaaaataatg ctagcatcgg taagagacga
    13081 aagttggtta gttgtaggaa atgacgagaa cgaatgtagt tttcgcttgg aagataatca
    13141 attattgata aaaaatgtat tgtctatctc tgttttcaaa gaaaagcaat ttttgattcg
    13201 agacttcatt cagaaaaaaa tgattgctca tggggtgttc gcctatatgc gagcttatag
    13261 cgagtttatc tatcacaata caaaacagat ctctcaacgg ctattgtttg agaaaaagga
    13321 tgaaatcgaa catttaccta aaatgaaaca acaaaacggg gaagtagtag tcgattgcaa
    13381 ccaatttcca ggatacgatc ttttttacca aggtttatgt ttcacttcgt gttgggaaat
    13441 gtattatagt cgctattatc atcaaatcat ccctaaaccg atttttctcg acatccaaca
    13501 agtagaaaaa gtgaaggaat tagaaaacga agtcatatgt atccagcttt atcgagaccc
    13561 ctttaactgg caaaagccaa acaatcaaat gtttcaatcc tattttaggg accagcttgg
    13621 gtttgatcat cttgcttggg acaatggtgt cggtttattg aaaccgccat tcgttgaata
    13681 catttacact gaccatacga tccaatctgt ccagtatcag aatgcacaaa tgcaacctgt
    13741 tcctaaaaag aatgcttcat tttttgtaac aaaaagttat gatattcaac aaggagatta
    13801 taaagaaagg cgtgttcgcg gaacactaaa tgctcaggcg tattttcctt gggttgacga
    13861 gaatcgctcg cgcatgatgt gctataaagt tatcgatccg actgttgctt tggataatgg
    13921 aatcgaagct tattgttatt atattaggga atatctagaa gtagaggtaa cagatgaaaa
    13981 atatcaagag tatcttttat ccttaagaat ctatgttcct tcagaatccc tgtcagaatt
    14041 accgttaaag gaaatcaaac atagactctc tgatgttacc ttcaaacgaa taaagaaaaa
    14101 gcggcgaagt atccaattcg atgtcaagaa aggtgagcag catctgcgtg tgcaattttt
    14161 agattaccga gaattagaac aattgattac tttacaaaaa atatgaggtg aagaaaatgg
    14221 ataaacaact ctattatcga ataatggttc cggcatcatt attatccgtc tgtctctttt
    14281 ttgttcctaa ttttcctatt cgatcagtca tcggtttttt ggtcatgttt ggcgggatgg
    14341 gactttatta ttatcttctt cataaggaaa gaaaacctgc ttctgtttat atgaaaaaaa
    14401 caagaagcga aaataaaaaa cgataaaacg gcttgatgat agacaa
//
LOCUS       DAYYAR010000069        14438 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000069 DAYYAR010000000
VERSION     DAYYAR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.069"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1115)
                     /locus_tag="ACHAT0_001742"
     CDS             complement(228..1115)
                     /locus_tag="ACHAT0_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8451681.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="ACHAT0_001743"
     CDS             complement(1112..1981)
                     /locus_tag="ACHAT0_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8451682.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="ACHAT0_001744"
     CDS             complement(2118..3404)
                     /locus_tag="ACHAT0_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8451683.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="ACHAT0_001745"
     CDS             3677..4255
                     /locus_tag="ACHAT0_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8451684.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="ACHAT0_001746"
     CDS             4255..5184
                     /locus_tag="ACHAT0_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451685.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="ACHAT0_001747"
     CDS             5181..6917
                     /locus_tag="ACHAT0_001747"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8451686.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHAT0_001748"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHAT0_001748"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8451687.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="ACHAT0_001749"
     CDS             complement(7813..8322)
                     /locus_tag="ACHAT0_001749"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8451688.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHAT0_001750"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHAT0_001750"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8451689.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="ACHAT0_001751"
     CDS             complement(9623..10714)
                     /locus_tag="ACHAT0_001751"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8451690.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHAT0_001752"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHAT0_001752"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8451691.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHAT0_001753"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHAT0_001753"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8451692.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHAT0_001754"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHAT0_001754"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8451693.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4112 a         3144 c         2609 g         4573 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc caaactga
//
LOCUS       DAYYAR010000070        14189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000070 DAYYAR010000000
VERSION     DAYYAR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.070"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..483
                     /locus_tag="ACHAT0_001755"
     CDS             <1..483
                     /locus_tag="ACHAT0_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8451694.1"
                     /translation="FGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIET
                     ALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNG
                     ILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI
                     "
     gene            805..975
                     /locus_tag="ACHAT0_001756"
     CDS             805..975
                     /locus_tag="ACHAT0_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451695.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            989..1591
                     /locus_tag="ACHAT0_001757"
     CDS             989..1591
                     /locus_tag="ACHAT0_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8451696.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1607..2284
                     /locus_tag="ACHAT0_001758"
                     /pseudo
     CDS             1607..2284
                     /locus_tag="ACHAT0_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2595..3092
                     /locus_tag="ACHAT0_001759"
     CDS             2595..3092
                     /locus_tag="ACHAT0_001759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFM8451697.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            3093..3509
                     /locus_tag="ACHAT0_001760"
     CDS             3093..3509
                     /locus_tag="ACHAT0_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFM8451698.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3529..5073
                     /locus_tag="ACHAT0_001761"
     CDS             3529..5073
                     /locus_tag="ACHAT0_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8451699.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5216..5440
                     /locus_tag="ACHAT0_001762"
     CDS             5216..5440
                     /locus_tag="ACHAT0_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451700.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5455..6324
                     /locus_tag="ACHAT0_001763"
     CDS             5455..6324
                     /locus_tag="ACHAT0_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8451701.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6305..7039
                     /locus_tag="ACHAT0_001764"
     CDS             6305..7039
                     /locus_tag="ACHAT0_001764"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8451702.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7072..7980
                     /locus_tag="ACHAT0_001765"
     CDS             7072..7980
                     /locus_tag="ACHAT0_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8451703.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7977..8519
                     /gene="sat4"
                     /locus_tag="ACHAT0_001766"
     CDS             7977..8519
                     /gene="sat4"
                     /locus_tag="ACHAT0_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFM8451704.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8612..9406
                     /locus_tag="ACHAT0_001767"
                     /note="aph(3')-IIIa"
     CDS             8612..9406
                     /locus_tag="ACHAT0_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8451705.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9682..9813
                     /locus_tag="ACHAT0_001768"
                     /pseudo
     CDS             9682..9813
                     /locus_tag="ACHAT0_001768"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9889..10382
                     /locus_tag="ACHAT0_001769"
                     /pseudo
     CDS             9889..10382
                     /locus_tag="ACHAT0_001769"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            10552..11448
                     /locus_tag="ACHAT0_001770"
     CDS             10552..11448
                     /locus_tag="ACHAT0_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8451706.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            11547..11756
                     /locus_tag="ACHAT0_001771"
     CDS             11547..11756
                     /locus_tag="ACHAT0_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8451707.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11773..12045
                     /locus_tag="ACHAT0_001772"
     CDS             11773..12045
                     /locus_tag="ACHAT0_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8451708.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12047..12910
                     /locus_tag="ACHAT0_001773"
     CDS             12047..12910
                     /locus_tag="ACHAT0_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8451709.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13173..13910)
                     /gene="erm(B)"
                     /locus_tag="ACHAT0_001774"
     CDS             complement(13173..13910)
                     /gene="erm(B)"
                     /locus_tag="ACHAT0_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8451710.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14035..14118)
                     /locus_tag="ACHAT0_001775"
     CDS             complement(14035..14118)
                     /locus_tag="ACHAT0_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8451711.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5018 a         2222 c         2928 g         4021 t
ORIGIN      
        1 tttggtcacc ttgaaggtga tacgatcgtt ggcattcatc ataaaagtgc cgtcattact
       61 ttagttgaaa gattatctaa agtcattatc acgattaaac ccaacggccg taaggcatta
      121 gatattgaaa ctgcccttaa tcaatggttt tctcgcttcc ctaaaaactt ctttaaatct
      181 attacgtttg actgtggaaa agaattttct aactggaaag ccattagtaa ccaacatgat
      241 attgatatat attttgcgga ccctggaaca ccttctcaac gcccattaaa cgagaattct
      301 aacgggattc tgcgtcgtaa tggactgccg aaatcaatgg attttagaaa agtgaatcag
      361 acatttattt ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac
      421 agaacaccaa tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt
      481 tgacaaatca tactatgata cgtgtttgtt tttctgtgct gtttagtgaa tgattagcag
      541 aaatatagag agtaagattt taattaatta ttagggggag aaggagagag tagcccgaaa
      601 acttttagtt ggcttggact gaacgaagtg agggaaaggc tactaaaacg tcgaggggca
      661 gtgagagcga agcgaacact tgatctttta agttgctatc tttttaaggt caatagagta
      721 tacttatttg tcctaatatg gttttagcag tataattgac ttggtgaata ggtcatttaa
      781 gttgggcata ataggaggag taaaatgaaa aaatttattt atcgagtttt agaaaatgac
      841 gaagtggtgg ctatttttaa tgagcaacaa tatgcgcaag attttatcgc ttacgaaaag
      901 acaatttctg ataagcaatt tgaaattgaa aaagtagata tagctgattg gttattgcaa
      961 ccgagagaat tttagaggtt ggttgaaaat ggctaaaatt ggttatgcac gtgtcagtag
     1021 caaagaacag aacttagata gacaactgaa agcgttagag ggcgtttcta aggtcttttc
     1081 agacaaatta agcggtcaat cggtcgaacg cccacaatta caagctatgc ttaactatat
     1141 tcgtgaaggg gatatagttg ttgttactga attagatcga ttaggacgaa ataataaaga
     1201 attaacagaa ttgatgaatc aaattcaaat taagggggca accctggaag tcttaaattt
     1261 accctcaatg aatggtattg aagatgaaaa tttaagacgg ctgattaata atttagtgat
     1321 tgaattgtat aagtaccaag cggaatctga acgcaaacga attaaagaac gccaagccca
     1381 aggaattgaa attgctaaga aaaaaggaaa attcaaaggg cgacaaccta aattcaaaga
     1441 aaatgatcca cgtttacaac acgctttcga tttatttttg aatggttgtt ccgataaaga
     1501 agttgaagaa caaactggaa ttaatcgccg aacgtttaga aggtatcgat caagatacaa
     1561 cgtgacagta gatcaaagaa aaaaacaatg aaaagaggga tagttaatga gtacggttat
     1621 tttagctgaa aaaccaagcc aggcattagc ctacgcaagt gctttaaaac aaagcaccaa
     1681 aaaagacggt tattttgaga tcaaagaccc actatttaca gatgaaacgt ttatcacctt
     1741 tggttttggg catttagtcg aattagcaga accaggtcat tataacgaaa agtggcagaa
     1801 ctggaaactt gaatctttgc cgatttttcc tgatcgatac gattttgaag ttgcaaaaga
     1861 taagggaaag cagtttaaaa ttgttgctga acttctcaaa aaggcaaata caattattgt
     1921 cgcaacagat agcgacagag aaggtgaaaa tatcgcctgg tcgattatcc ataaagcaaa
     1981 tgccttttct aaagataaaa cgtataaaag attatggatc aatagcttag aaaaagatgt
     2041 gatccgaagc ggttttcaaa atttgcaacc aggaatgaat tactatccct tttatcaaga
     2101 agcgcaaaca cgccaaattg ccgattggtt gatcggcatg aacgcaagcc ctttatatac
     2161 cttgaattta caacaaaaag gcgtacaagg tacattttca ttaggacgtg tacaaacacc
     2221 gactttatat ctcatttatc aacgccagga agcaatagaa aactttaaaa aagaaccttt
     2281 tttcctgaat aattcatagt tttactaata gtctttccta acattgattt aatagtcttt
     2341 aaagtattct gcttcatcac ccattaggtg atgaaaaaag aacccctttc tgatttagcg
     2401 gacaaatact ttgacctaat ctcagaagat ataaaagaaa aaataataaa atatccgatt
     2461 tatactttgt ataaagaact aaagtagtta gcaaatcatt tacagcaaat cactatgttc
     2521 cacataccat tttacatact taccttatcc acgacacatg ttgagatagt atcgaagata
     2581 aagaaagttg ctccatgttg tttttgcgag atggggaaat caaagccaca ttgacaacca
     2641 tgatgaataa gcttgctttc agtcataagc taatcctaga accacttttc aagtcagtta
     2701 gccaaatcga tgaagaaagc gaccgtgaaa gaatggacgc tattgataag ctaatggagc
     2761 aactattgga agaacgcaat acccttattg cactgatgtc caaaggtttc ctagaacctg
     2821 ctctttttaa tcaggaacga aatgttttag atagtgagat taaaaatctt actaccgaaa
     2881 aaacaaacct ggtaacgaac tctgcgagtg gggttttgcg agcaaacgag ataaaggacc
     2941 tcattaatta tgtgtcagca gataatttta atggtgacta cacggaagaa ctatttgaag
     3001 aatttgtagt gaacatcatt gtaaattcca gggatgagct gacattcaat ttgaaatgcg
     3061 gtctttccct gaaagaaaag gtggtgagat aaatggcata tattccatat ggatacaaaa
     3121 ttcaagatgg agtggttact gtcgatgaaa aggcagcagg tcaagtaaag gtattctttg
     3181 agaaatacat atcaggacta tcccttacag tggctggcga acaggcaggt attgaaaaga
     3241 cacattctgt gatggggcgc attttgaaaa acgtcaacta ccttggaaat gatacgtatc
     3301 cagcaattat tgataaagag atatttgata aagctgaaga agttagagat aaacgtgcaa
     3361 aggatttagg acgagtggta gagcttgccg ctttcacctc tccccctccc aaagaacgat
     3421 tcaaaatgaa aaaggcagat aataagatgc cagtggatcc ttttgaacga gcagaatact
     3481 tatacagtct gatagaaagc gaggaataaa gtgacagaga aaaatataat ggttattcct
     3541 gctcgtaaaa gagtaggaag tacagccgca aaagaaaaga taaagaaact tcgtgttgct
     3601 gcctattgcc gtgtttctac agaaacagaa gaacaaaatt ctagctatga ggttcaggtc
     3661 tcacactata cagagtttat aaagaaaaat aatgaatggg agtttgctgg catctttgca
     3721 gatgatggta tctctggtac gaacactaaa aagcgtgacg aatttaatcg catgattgca
     3781 gagtgtatgg acggtaacat cgacatggtt attacaaaat ccatcagccg atttgcgcgt
     3841 aacaccctag actgccttca atacattaga cagctcaagg ataagaacat atcagtttat
     3901 tttgagaaag agaacatcaa caccacggat gccaaaggtg aggttttgct gactattatg
     3961 gcatctttgg cacagcagga aagccagagc ctttcacaaa acgttaagct tgggctacag
     4021 taccgatacc aacaaggaaa ggtgcaggtc aaccataagc gatttatggg ctacacgaaa
     4081 gatgaagatg gaaatttaac tattgtcccc gaagaagctg agattatcaa acgcatctac
     4141 cgagaatacc ttgagggtca gagtctagtg ggcattggtc gagcccttga aaaggatggt
     4201 attttaacag cagcgggaaa accaagatgg cgaccagaat cagttaagaa gatacttcaa
     4261 aacgaaaaat acatcggaga tgcccttctg caaaagactg tcacagtaga ttttctgacc
     4321 aagaaacgag tgaaaaatga agggcatgtt ccccaatatt atgttgaaaa tagccatgaa
     4381 gcgattattc ctaaagagtt atttttgcag gcgcaagaag aaattcatcg gagaagcaat
     4441 atctacacag gagaaggcaa gaacaaaaga atttacagta gcaagtacgc tttaagtgcc
     4501 atcaccttct gtggagattg tggcgatatt tatagacggg tctattggaa tatccacgga
     4561 agaaaagaat ttgtctggcg atgcgtgact agaatcgagc aaggtcctga agtttgtaag
     4621 aaccgaaccg taaaagaaga tgaactttat ggtgcagtaa tgactgcgat taataagcta
     4681 cttgcaggtg ggaacaatat gataaagaca ctggaagaga atattcatgc tgtgattggc
     4741 gaaaccacag aataccaaat ttcagagatt aacaactcac tagaggaaaa acaaaaagaa
     4801 ctaatcaagc tggcgaacaa gggtcaagac tatgaccact tagcagatga gattgatgag
     4861 ctgagagaca agcgacagat ccttttagta gaagatgcct ccctcagtgg cgagaacgag
     4921 cgaatcaatg agctgattga atttatccgc aagaacaaat tccgcacctt agaatatgat
     4981 gataagcttg taaggaagat aatccagagc gttacagtct atgaaggcca cttcgtcata
     5041 gccttcaaat ctggcatcga aatggaaata tgaaaaccag aagtaaatag cccatgactc
     5101 tacagtagag ttgtgggttt tcttttgctt gctgattata ataatatttt aataactatt
     5161 gtgtttatca accaaatggt ttataatatt cttagcgtaa acttggaggt gctgtatgac
     5221 tacggcagaa atgattaaag aactgtgtga gcaaatgaat ataagtgttt ccgaacttgc
     5281 tagacgtatt ggccagactc cacagaattt caataaaaaa ttacaacgag aaacagtaac
     5341 cttggatgag ttgaaggcca ttgctgatgt gctaggtgtc aagtttgtgc aggcatttat
     5401 tttaccagat ggagaagaaa taaaaatatc taacgaataa aggaggatgt ttatatggta
     5461 ggtaatgtta ttcaaatagt aacagagaaa ttatcatctt tgccttttat agaaggcatc
     5521 gtattagggg gctcacgtgc aagaggcacc catacagaaa attctgatat agatatcgga
     5581 atctattaca attcagactc atttgacctg acagcgatta atcaaattgc tacagagttg
     5641 gatgatgaga atagaaacaa ccttgttgta cctcccggag cgtggggtga ttgggttaat
     5701 ggcggtggat ggttagttat aaacgggtgt catgttgact tgatattacg tgatataaaa
     5761 cgggtggaac aaataatcaa agatacggag caaggaattg ttactgccaa ttatcagacc
     5821 gggcatcccc atggttatat aagtgctatg tatcgtggag aattagcgat tagtaaaata
     5881 ctatatgcta agaacgaaag cttatgcgaa ttaaaaaagc aggcagaaat ttaccctact
     5941 gctctaaaga aaagcttgat gaactttttt atatttgaag ctgaattctc tttaatgttt
     6001 gtaaaagcaa atgcgggggc agaggataaa tattatattg caggccatgt ttttcgtatt
     6061 atttcatgct taaatcaagt actatttgca tgtaataatg cttattgcat taacgaaaag
     6121 aaagctataa aactgcttga aacttttgaa tataaacctg aaaaatatgc cgaaagagtt
     6181 aatcatattt ttgaagtact cggtctttct ctttttgaat gctacgacat gaccgagaaa
     6241 ctttataaag aagtgaatga aattgtatcg gagataaata actttttaaa cgaggagagt
     6301 tcagatgaaa gaaaacaaat atgatgataa tatatttttt caaaaataca gtcaaatgag
     6361 tcgctcgcag aaaggactgg ctggtgcggg agaatgggag actttgaaaa agatgctacc
     6421 tgattttaag ggtaagcgtg tgcttgattt aggatgcggc tatggatggc actgtatata
     6481 tgcgatggaa aacggtgctt cctctgtagt aggtgttgat atttctcata aaatgctcga
     6541 agtagcaaaa ggaaaaaccc attttccaca gattgaatat gaatgctgtg ccatagaaga
     6601 tgtggatttc ccagaggaga gctttgatgt aatactaagt tcgcttgcgt ttcattatgt
     6661 agcagactat gagaatttaa taaaaaagat atataggatg ctgaaggctg gtggcaattt
     6721 agtttttaca gttgaacatc ctgtttttac tgctcatgga acacaagact ggtattataa
     6781 cgaaaaagga gaaatactgc atttcccggt ggacaattat tattatgagg gcaaacggac
     6841 agctatgttt ttggaagaaa aggttacaaa atatcataga acactgacca catatctaaa
     6901 tacactgctt tcaaatagtt ttataataaa tcagattgtg gagccacagc cgccagagaa
     6961 catgatggat attccgggga tggcggatga aatgcgacgc ccaatgatgc tgattgtatc
     7021 ggcaaaaaag aagatgtaat aatatagaaa aaataaacga ggagtatgta aatgagatca
     7081 gaaaaagaaa tgatggattt agtactttct ttagcagaac aggatgaacg tattcgaatt
     7141 gtgacccttg aggggtcacg cgcaaatatt aatataccta aagatgaatt tcaggattat
     7201 gatattacat attttgtaag tgatatagaa ccgtttatat ctaatgatga ctggcttaat
     7261 caatttggga atataataat gatgcaaaag ccggaggata tggaattatt cccacctgaa
     7321 gaaaagggat tttcctatct tatgctattt gatgattaca ataaaattga tcttacctta
     7381 ttgcccttgg aagagttaga taattaccta aagggcgata aattaataaa ggttctaatt
     7441 gataaagatt gtagaattaa aagggacata gttccgactg atatagatta tcatgtaaga
     7501 aagccaagcg caagggagta tgatgattgc tgcaatgaat tttggaatgt aacaccttat
     7561 gttattaaag gattgtgccg taaggaaatt ttatttgcta ttgatcattt taatcagatt
     7621 gttcgccatg agctgctgag aatgatatca tggaaggtcg gcatcgaaac aggctttaaa
     7681 ttaagtgtag gcaagaacta taagtttatt gaaaggtata tatccgagga tttgtgggag
     7741 aaacttttgt ccacctaccg gatggattcc tatgaaaaca tatgggaagc attatttcta
     7801 tgccatcaat tgttcagggc ggtatccggt gaggtggcgg aaaggcttca ttatgcctat
     7861 ccggagtatg ataggaatat aacaaaatat accagggaca tgtataaaaa atacactggt
     7921 aaaaccggct gcctggatag cacatatgcc gctgatatag aagagaggcg ggaacagtga
     7981 ttacagaaat gaaagcaggg cacctgaaag atatcgataa acccagcgaa ccatttgagg
     8041 tgataggtaa gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct
     8101 atgaagcgcc atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag
     8161 attgccttga atatattgac aatactgata agataatata tctttactac caagacgata
     8221 aatgcgtcgg aaaagttaaa ctgcgaaaaa attggaaccg gtacgcttat atagaagata
     8281 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
     8341 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
     8401 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
     8461 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     8521 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     8581 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     8641 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     8701 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     8761 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     8821 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     8881 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     8941 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     9001 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
     9061 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     9121 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     9181 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     9241 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     9301 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     9361 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     9421 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     9481 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat agagttctat
     9541 attagagatg taaagagttt tggtgaagag gtttgtgagt atggctttta taaccagggt
     9601 gcaatgagaa gcactaacag cagctagctt aagaacgcat aggatatact ttttatggaa
     9661 gtcacagagg aggaataaaa tttgaacagg attaacagag taacctctat tcttattcag
     9721 ctgcaatcaa aaaaaataat tcctgccaaa gaaattgcac agcgatttaa tataagctta
     9781 aggacagttt acagagatat ccggacactt gaagaagccg gaataccaat tggatctgag
     9841 gccggaaaag gatattttct tgtagagggc ttcctacttc cgccggtaat gtttacagcg
     9901 gcggaagtgg gtgcattgat tacagcaggg aaatttttaa attgccatgg agatgaatca
     9961 tttataaagg attttgattc agctatgtat aaaatcaaat ctattttgaa gcatggtgaa
    10021 aaaaactatg cacaggagtt ggagaacagt attaacgtgt acagcacatc tggacagaaa
    10081 aatacattgg cggataacgt tattgcagca attcagactg caatctgcaa taaaagggta
    10141 atatcaattc aatatcctgc atcaggaggg caagaaccgg aaagcagaat gatagagcct
    10201 atatcactgg ggttttatga acagaactgg tacttaatcg gttttgcagg ctaagaaatg
    10261 aatataggaa ttttcgtgtg gatcggatta aaagcatttg catagaagaa gagctctgtc
    10321 aaagccatga tggctctttg gaacaaatat taaaacaaat gctttcatat aaaaaactat
    10381 atgccctaca atgccgttgt aaaaattggg gaaaagggat atattgattt tatatctttc
    10441 tcaaaataaa tataaaagcc ctttaaagag ggcttttata tattaatcac aaatcactta
    10501 tcacaaatca caagtgatta atcacaaatc acttgtgatt tgtgattctt aatgatacaa
    10561 tattactata caaaaaaaga atggggcgta gttatggaga aggaaaaact aaaaatactt
    10621 gaagaattaa gacgtatttt aaacaataaa aatgaagcaa ttattatctt gaataattac
    10681 tttaaaggtg gtgttggaaa gtccaaatta tcgactatgt ttgcttactt gacagacaaa
    10741 ttgaatttaa aagttttaat gatcgataag gacttacagg caacattgac aaaagactta
    10801 gcaaaaactt ttgaggtaga attgccacgt gtcaattttt atgaaggctt gaaaaatgga
    10861 aacttggctt cttctattat tcatttgact gataatttag acttgatccc tggcacgttt
    10921 gatttgatgt tactgccaaa attaactcgc tcatggacgt ttgaaaatga aagtagattg
    10981 cttgctactc ttttagcacc tttaaaaagt gactatgatc ttattattat tgatactgta
    11041 ccaacgccaa gcgtttatac aaataatgca atcgtggcga gtgattacgt tatgatccct
    11101 ttacaagcag aagaagaaag tacaaacaac attcaaaact atatttccta tttgattgat
    11161 ttgcaagaac agtttaaccc tggactagat atgattggtt ttgttcctta tttagttgat
    11221 acggacagcg caacgataaa atcaaacctg gaagaactgt acaaagaaca taaagaggat
    11281 aatttggttt tccgaaatat tatcaagcga agtaataaag taagtacttg gtctaaaaat
    11341 ggcattacag aacacaaagg ctatgacaaa aaagttttgt ctatgtatga gaacgtattt
    11401 tttgaaatga ttgagcgaat cattcaatta gaaaatgaaa aagaatagaa tcacaaatca
    11461 caagtgatta atcacaaatc acttgtgatt tgtgataggt gatgataaaa taaatagtaa
    11521 gaagaaatag aaagaagtga gcgatcgtgg gaaatttagg cgcacagaaa gcaaaacgaa
    11581 atgatacgcc aatcagcgca aaaaaagata taatggggga taagacggtt cgtgttcgtg
    11641 ccgacttgca ccatatcata aaaatcgaaa cagcaaagaa tggcggaaac gtaaaagaag
    11701 ttatggatca agccttagaa gaatatatac ggaaatattt acctgacaaa ctttaaaagg
    11761 agtgaaaatg aaatggcagt tacgtatgaa aaaacatttg aaattgaaat cattaatgag
    11821 ttatcggcaa gtgtttataa tcgggtatta aattatgttt tgaatcatga actagatact
    11881 aaaaatactc gtttactaga agtgaatctt ttaaatcaat tagaagtggc acaagaagtt
    11941 gatttatttc aacaaccatt tgaagaatta caagctattc atgagtattg gcggtcaatg
    12001 aatcaatatt caaaacaaat tttgaataaa gagaaagtgg cttaacatgg cgaatattac
    12061 tgacttcacc gaaaagcaat ttgaagatcg tttagaaaag aatgttgaac gactaactaa
    12121 aaatagacta gcggttgaat cgccaaccgc ttttttactt ggtgggcaac cagggtcagg
    12181 gaaaaccagt ttgcgatcag caatttttga agaaacacaa gggaatgtta ttgtcattga
    12241 taatgatacc tttaaacaac agcacccaaa ttttgacgaa ttagtgaaac tctatgaaaa
    12301 agacgtagta aaacacgtta ccccttattc gaatcgcatg acagaagcac ttattagccg
    12361 tttaagcgat caaggctata atttagtgat tgaaggaaca ggacgaacaa cagacgttcc
    12421 cattcaaaca gccacaatgc ttcaatctaa aggttatgaa acaaaaatat acgtcatggc
    12481 agtaccgaaa attgaatcat acttaggaac gattgaacga tatgaaacca tgtatgcaga
    12541 tgatccaatg acagctaggg caacaccaaa acaagcgcat gatattgttg tcaaaaactt
    12601 accgaccaat ttagaaaccc ttcataaaac gggcttattt agcgatataa ggctttacaa
    12661 tagagaagga gtgaaactct attcaagctt agaaacgcct tccattagtc cgaaagagac
    12721 cttagaaaga gaattaaatc gtaaagtatc agggaaagaa attcaaccga ctttggagag
    12781 aatagagcaa aaaatggttc aaaatcaaca ccaagaaacc cctgaattta aagcaattca
    12841 acaaaaaatg gaaagcttgc agctacctac accaccaata cccaaaacac ctaaacttcc
    12901 aggtatttaa accttaaaaa aggaaaagag tagttaccaa aaaacggtaa ctactctttt
    12961 tttataaaaa catttcctca tgttggggtc ccgagcgcct acgaggaatt tgtatcgata
    13021 agaaatagat ttaaaaattt cgctgttttt ttctaatttc attttgaggt gaattaattg
    13081 gtagttatta gaggtgccct atacctaata atttatctac attcccttta gtaacgtgta
    13141 actttccaaa tttacaaaag cgactcatag aattatttcc tcccgttaaa taatagataa
    13201 ctattaaaaa tagacaatac ttgctcataa gtaacggtac ttaaattgtt tactttggcg
    13261 tgtttcattg cttgatgaaa ctgattttta gtaaacagtt gacgatattc tcgattgacc
    13321 cattttgaaa caaagtacgt atatagcttc caatatttat ctggaacatc tgtggtatgg
    13381 cgggtaagtt ttattaagac actgtttact tttggtttag gatgaaagca ttccgctggc
    13441 agcttaagca attgctgaat cgagacttga gtgtgcaaga gcaaccctag tgttcggtga
    13501 atatccaagg tacgcttgta gaatccttct tcaacaatca gatagatgtc agacgcatgg
    13561 ctttcaaaaa ccactttttt aataatttgt gtgcttaaat ggtaaggaat attcccaaca
    13621 attttatacc tctgtttgtt agggaattga aactgtagaa tatcttggtg aattaaagtg
    13681 acacgagtgt tcagttttaa tttttctgac gataagttga atagatgact gtctaattca
    13741 atagacgtta cctgtttact tattttagcc agtttcgtcg ttaaatgccc tttacctgtt
    13801 ccaatttcgt aaacggtatc ggtttctttt aaattcaatt gttttattat ttggttgagt
    13861 actttttcac tcgttaaaaa gttttgagaa tattttatat ttttgttcat gtaatcactc
    13921 ctgaagtgat tacatctata aataaataca gaagttaaac gatttgtttg taattttagt
    13981 tatctgttta aaaagtcata agattagtca ctggtaggaa ttaatctaac gtatttattt
    14041 atctgcgtaa tcactgtttt tagtctgttt caaaacggta gatgttttat ctacattacg
    14101 catttggaat accaacatga cgaatccctc cttcttaatt acaaattttt agcatctaat
    14161 ttaacttcaa ttcctattat acaaaattt
//
LOCUS       DAYYAR010000071        14129 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000071 DAYYAR010000000
VERSION     DAYYAR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.071"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHAT0_001776"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHAT0_001776"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8451712.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHAT0_001777"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHAT0_001777"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8451713.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACHAT0_001778"
     CDS             8118..9086
                     /locus_tag="ACHAT0_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8451714.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACHAT0_001779"
     CDS             9104..10009
                     /locus_tag="ACHAT0_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8451715.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACHAT0_001780"
     CDS             10006..10812
                     /locus_tag="ACHAT0_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8451716.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACHAT0_001781"
     CDS             10969..11937
                     /locus_tag="ACHAT0_001781"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8451717.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACHAT0_001782"
     CDS             complement(12254..12778)
                     /locus_tag="ACHAT0_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8451718.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14129
                     /gene="scm"
                     /locus_tag="ACHAT0_001783"
     CDS             13065..>14129
                     /gene="scm"
                     /locus_tag="ACHAT0_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8451719.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4001 a         3048 c         2787 g         4293 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tcttttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 catcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggcgaa aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggagtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 atgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatca
//
LOCUS       DAYYAR010000072        13940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000072 DAYYAR010000000
VERSION     DAYYAR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.072"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..587)
                     /locus_tag="ACHAT0_001784"
     CDS             complement(69..587)
                     /locus_tag="ACHAT0_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8451720.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(589..1206)
                     /locus_tag="ACHAT0_001785"
     CDS             complement(589..1206)
                     /locus_tag="ACHAT0_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8451721.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1203..2057)
                     /locus_tag="ACHAT0_001786"
     CDS             complement(1203..2057)
                     /locus_tag="ACHAT0_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8451722.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2054..2965)
                     /locus_tag="ACHAT0_001787"
     CDS             complement(2054..2965)
                     /locus_tag="ACHAT0_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8451723.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3006..4340)
                     /locus_tag="ACHAT0_001788"
     CDS             complement(3006..4340)
                     /locus_tag="ACHAT0_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8451724.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4548..5378
                     /locus_tag="ACHAT0_001789"
     CDS             4548..5378
                     /locus_tag="ACHAT0_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8451725.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5622..5871)
                     /locus_tag="ACHAT0_001790"
                     /pseudo
     CDS             complement(5622..5871)
                     /locus_tag="ACHAT0_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6062..6256)
                     /locus_tag="ACHAT0_001791"
     CDS             complement(6062..6256)
                     /locus_tag="ACHAT0_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451726.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6761..6946)
                     /locus_tag="ACHAT0_001792"
     CDS             complement(6761..6946)
                     /locus_tag="ACHAT0_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451727.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7796..8137)
                     /locus_tag="ACHAT0_001793"
     CDS             complement(7796..8137)
                     /locus_tag="ACHAT0_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8451728.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8137..8367)
                     /locus_tag="ACHAT0_001794"
     CDS             complement(8137..8367)
                     /locus_tag="ACHAT0_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8451729.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8654..8992
                     /locus_tag="ACHAT0_001795"
     CDS             8654..8992
                     /locus_tag="ACHAT0_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451730.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9197..9759)
                     /locus_tag="ACHAT0_001796"
                     /pseudo
     CDS             complement(9197..9759)
                     /locus_tag="ACHAT0_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(10491..11072)
                     /locus_tag="ACHAT0_001797"
     CDS             complement(10491..11072)
                     /locus_tag="ACHAT0_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8451731.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11255..11881)
                     /locus_tag="ACHAT0_001798"
     CDS             complement(11255..11881)
                     /locus_tag="ACHAT0_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8451732.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11903..13234)
                     /locus_tag="ACHAT0_001799"
     CDS             complement(11903..13234)
                     /locus_tag="ACHAT0_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8451733.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13389..13790)
                     /locus_tag="ACHAT0_001800"
     CDS             complement(13389..13790)
                     /locus_tag="ACHAT0_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8451734.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
BASE COUNT         4307 a         2817 c         2317 g         4499 t
ORIGIN      
        1 ttcctcgaaa caaaaaaata aagcacaaat gcgcatttac gcactcatgc tttattcgta
       61 caattaattc agataacggc agcgcatagg atgccaataa cacttcctaa aatagctcct
      121 cctaaaatgt cagtaggata atgtaaaagt aaaaaaatcc tcgaataagc gatcaaacaa
      181 gcaaataaaa aggctaatat tcctgcaact ggacttaatt cgaacaacgc agctactgat
      241 aaaaaggcta gcgctgtatg tcctgaagga aaagaagaag tcttcggttt acttgtgtaa
      301 aggacaagcc gacgattttt ttcataaggt cttttccgtc ttgtcgtttt tttgataaaa
      361 agcaaaccaa caaaaaaata gatcaaactg ccgataagaa tggctagacc atattctcta
      421 gtatcaggta tcaagactaa aacagcagca aggagcaacc aaataaaacc accattagag
      481 atattactga taaatttcaa aaaattgatt tttcgactat ccgttttccg ttgcttggcg
      541 aacttgttga taatactatc atcgatttta gcaatcaaac gcaacatgtt attcctcctt
      601 tttaagaacc ttttcttgcg tacgatggat aaaaagcagg acttgaaaat aaaacaagaa
      661 actgaaacct aataaagcaa aatagattgg tcctgcaaac cagaatgcga caagaaggat
      721 caacgtgcct gccaaataga aaagcgcgct tttgaaatca ttaaaaaaca agaaaaatgc
      781 agtaatccaa gaagcttctt tttgttccag ttgaagcaag attttgtaac taaagagaag
      841 cgcgcttaat agaagcaaaa acaacgtagt atacatcaat gtatagccaa ttcccccacc
      901 ccaatagcgg aaaatctgat aatcgatcaa tagaagaaga aaataaacac ttgctaaact
      961 atcttgcttt ccataaagag agaagttctc acgaaacttt ttccagaata gtgaaccgag
     1021 ctttttctct tcattttttt tgtaatgata aatcatatac agaactgttc gacttgcagt
     1081 tcctaatgtg aaaacaggca agacgcacag gaaccatagt agctgtaaat ggataagacg
     1141 tggaatccat tcaatgattt ctaagaaata atcattggct tttttaccaa tcgatcgatt
     1201 catcatcctt ttgaacctcc tgtcaaatcc atcccttcaa tgaaataacg ttgtgcaaaa
     1261 aagaacagca aaatcgtcgg gatcaagaca agtgttgctc ctgccatcag ataattccat
     1321 tgtgtcgttg cttggttctg aaaaacttgc aagccaatct gtaacgtgta tttttcttga
     1381 tcttggatat ataacagagg acctaagaaa tcattccaag ctgcgttgaa ggaattgatc
     1441 gcaatggtaa tcaaggcagg tttcgtcaat ggaagcatca aatgactcca aatatacaaa
     1501 tgattcgctc cgtcgatttc agcagcttcg atcaattcat tattgatcga taagtaaaat
     1561 tgacgcatca gaaatatatt gaacgcattg ccgaagaacg atggaacgat caggggcaaa
     1621 taagtaccta cccagccgat cttggagaat aatacatatt gagggatcat cgttacaaac
     1681 cccgggatca tcatcgtaga aagaaccaaa gcaaacatca gctttttccc aggaaaatca
     1741 agcttcgcaa atccataagc aataaaagaa tttgaaagga cattaccgat cactactagc
     1801 acagtgatga ataatgtatt tcttgcatag cgccaaaaag gaaaggcggc aatcgtatcc
     1861 agatagtttt cccatacgat tttttctggg aaaaaggtcg gaggataacg aacgatttct
     1921 tccattggtt tcaatgaagt aaacaccatc caaagcaatg gaatgatcaa aatgattcct
     1981 attactgtca tgaggacata acttagtatt ttttttgttt gccgattcac actggtcttt
     2041 ttgaaataat ccatcatttg ccgtcacctc cagaataata gacccactta ggtgcaagct
     2101 tcaaattgac aaacgtcaga accaacacga tcagaaacag gatcattgac atagccatcg
     2161 cataacccat atcaaaagct acgaacgcct tgttccacat atgcagatta taaaaaagca
     2221 aagaatttgc aggtccgcct gttccatttt tcgtcatgac ataagcttct tgaaaaattt
     2281 ggaaagaacc gatcgtagag gtgatcacat cgtaaaaaat aataggagtg attaatggca
     2341 aagtaatact tttgaacttt ccccaaacgc ctgctccatc gatttctgct gattcatata
     2401 aagatctagg aacattttgt aatgtagcta agtaaagtag cattgctcct ccagaactcc
     2461 atattttcat caaaatcaac gcaggtttcg tccattgtgg atcaaacagc caagctggtc
     2521 cagggatatg gaaccagccc agaaatgtgt tgatcaatcc tgcagatgga gaaagcagct
     2581 gcatccataa gatgtagact gctactcctg ataagactgc cggtaaatag aaagctgtac
     2641 ggaatacact gattcctttg attttttgat tgagtactgt ggctaaaaac acactgaaaa
     2701 tcgtcgtcag cggtacattg aagatgacga aataaacggt gttgtataac gatttccaaa
     2761 ataaatcatc gtgaacgaac attcttttga aatttgctag accaatgaaa ttcatcttgg
     2821 atgtcaaatt ataatcggtg aaactaccaa taactgaaaa aagcataggc cctagcataa
     2881 ataccaagaa cccgataata aaaggcgaga taaataaata ggcccatctt gcttcttttt
     2941 tctttcggat agataaacgc ttcaaatttt cttgcctcct ctgtacctag acagtcaaaa
     3001 atggattatt gattttgctt gactaagttt tctactgctt tttgtgcatc tgccaatcct
     3061 tcttttggtg attttgttcc taaaagaatc tggtcaatct gttcattcac taatgtcgtg
     3121 taatccggag catctaaagg aactggtgtg atcaccgtat ttttaaagtt ttcgtccgcc
     3181 atttgataga tcatttttcc tttatccgtt aggttgtcat tattcactaa attctcattg
     3241 gctgtcttgc tcgccatgat atcaaaactt ttttcgccga atttttcttg tacttctgga
     3301 gaagaaagat atttgatgaa ttcataagag gcctcaggat cttttgctcc gtatggtact
     3361 tccaagacga atccgccacc ccacgaccaa tggcctgaac cttcttcttt ttctgggagc
     3421 tgcatcacgc caaaatcaaa atcatccggt gcattttcca tcaaactaga gtaataattg
     3481 atattttggg cacgcatggc tactaatccg gaaataaatg gatcagatac accagaccca
     3541 aattcggctt ctaatttatt gatcgtattt ttcccataat aatcttgcca gtcttggatc
     3601 cactccaagg cttccacttt atttttagta tcaatcttga ctttttcatc tttgtcaaac
     3661 caactagtcc cgttatctgc gtttagcgcc catacatcag cccctaaatt ccataatgga
     3721 taaaaaccga tgcgtgtaaa ttcttttcct tcttttttat ctagtttacg tgctgtttct
     3781 tctaattcat cccaagttgt cggtagatct tgttcactga ttccagcttc tttaaataac
     3841 tttttattat aaaaaatgac ttgtgtatct gtattgaatg ggatcgcata aggatcacct
     3901 tcatattcta ctgtatccca aagctgcgga taaaaatcct tagaagtgtc tttttctaca
     3961 tatttggaga gattagttgc ttgctgtgct tctgctcttt gggcaacgga atttatgtcc
     4021 tgtacaataa catcgggtgc atttccagct gttattgcag ataaagattt agtccagatg
     4081 tcaccccatg gctgatactt ataatttaca tggatctcgt tttgtgattg attgaaatcc
     4141 tcaatgattt cttcgatagc ttcttttcgt gcaccggatc cccaaaagga ccagaaatca
     4201 atattttttc ttccatcact tgttttttca gaagaatttg ctgcacttga actggcatcg
     4261 cttgcattac ttgatccgca tcctcctaat aaaaacaatg ctgaacttgc cagcaaccct
     4321 gtaaacaata attttttcat attttacctc caaattttaa tgtaaccgat gccggtttta
     4381 atataacgat atcattttgt tctgaaaata acaatttata aggtatgatt ctaaaaaaat
     4441 tattaatgat accaatactt tatataattc gcgagcgctt tctttgaatt ttttaatctt
     4501 tcgtagcaga atgagaatgt attaaaaata gattaggagg aattttgatg tttagacacc
     4561 agaaagaatt gcagtttgaa gcaaaaccag atcgtccgaa tccattaatc gcgaaatatc
     4621 tgcaggagct gatcggcggc caatatggcg agatgagtgt agctatgcag tacctgttcc
     4681 aaggctggag ctgccgagga gaagaaaaat ataaagatat gctgatggac attgcaacag
     4741 aagaaatcgg ccacgtggaa atgttggcga caatggttgc tcgtttatta gagggtgcgc
     4801 ctgttgaaga tcaagaagca gcgatgcaaa aagatccaac aatcggtgcc attatctctg
     4861 gaatgaatcc acagcacgcg atcgtagctg gcttaggacc tcgtcctgct gacagcgtgg
     4921 gcaatccatg gagcggtgcc tatatcattg cgagtggcaa cttgttagca gactttagag
     4981 ctaatttaaa tgccgaatca caaggaagac ttcaagtagc acgtatttac gaaatgatcg
     5041 atgatcgtgg cgtaaaagat atgctgtctg ttctccttgc aagagatagc tatcatcaaa
     5101 atctatggga atgtgcagtg aaagaattag aagaaaaaga aggttcaatc ttagtcccaa
     5161 gtactttccc gcgtgacaaa gaacgaaaag acattgctta tgacttctat aacttctcag
     5221 agggagacca aagtagtgaa ggttcttggg ctagtggcaa agcactggat ggcgaaggcg
     5281 aatatcaatg gttgaaagaa cctaagatcg aaggcgctgc accaaaatta gaaccagtaa
     5341 caccaaaaat gtatgggaca cctccattca agaaataaat atctacccgt agaaacaccc
     5401 gttttttgtt ccacacgctt tgtgagcaaa actgactaaa gtcattaatc ataaccaccc
     5461 ttcaaaaggg tggttttctc tagagctata agctcaaatc accggccagc gcctaaagac
     5521 gctggctttc atattttatg ttcaagccat attgcctttg ccagttttgc ccacctctta
     5581 aaaggtttat gtactactcc ttagccctta aaaggatcgt cttactctcg tacactcagc
     5641 ttatctaacg cactatcatg tttttcttgt tcttgaatgt actttttgat ggtcgcttca
     5701 ttttgtccta cttttgtcac atagtatcct tccacccaaa agtgtcgata cccaaattta
     5761 tattttagat ttgcacaatc tcttgatgat ctcctgaata ttggctcgat attgattata
     5821 aattattttt cttctatact ttggtgtgaa tacaatgtgg tatttacaca tctactttgt
     5881 acgggctaaa ctatttccct ttattgctcc acccgcatag caagtggttt tgtatttcaa
     5941 tcgctcggcg cgctcaatta ggtctaaaga caataaatct aaaaacccgc tgcttcactg
     6001 acagaaacaa cgggttttca gaaaaaaatg aaatgaaaaa gatctcttac ggcaatctat
     6061 tttatttttt tgatttttta taaatggaaa atgcttcaag aagtccaaga aataaagccg
     6121 tataaaaaca agaacctatg aaatctttat ttattagata ttcaacagaa atcccgataa
     6181 cactagccaa caatatattt atcagaagtg taaaataagg atgcttttta tagaatagaa
     6241 ctagtgttgt tttcatagac tctcctttct tcatatttcc aattaatcac aaattcacgg
     6301 cattactaga aaaattaaag gggcgcagaa gtggaaaaat actcttcgac tttccctaca
     6361 aaccaggcca tcttgtgttt aataactata ccaaatctaa ggtacctcta aatctaaatt
     6421 ccatttgtgg cggtgacctc caatttttaa ctcttctatc tcccatccag tatacataac
     6481 cttgatactt cgaccactta tatatcgaac acacaaatac ctatccattg caggagtact
     6541 acctaactta gttgctactt gcatctaaat aacagctgaa aagataggac ttactcttag
     6601 taaagtatct gaactattaa tttttaaact atcattttta tcctcaacta catgcacatc
     6661 tgttgcaact tcactttctg tctcatccag ataaatgatt aaggaatcgg gaattgcaat
     6721 atcttcagct gacgctggaa tagttgagct aaattagtca tcatatcttt attataaaat
     6781 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
     6841 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
     6901 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
     6961 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
     7021 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
     7081 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
     7141 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
     7201 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
     7261 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
     7321 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
     7381 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
     7441 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
     7501 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
     7561 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
     7621 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
     7681 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
     7741 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
     7801 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
     7861 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
     7921 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
     7981 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
     8041 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
     8101 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
     8161 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
     8221 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
     8281 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
     8341 ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag tatactatat
     8401 aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag
     8461 tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa
     8521 aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta
     8581 accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac
     8641 aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa
     8701 atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa
     8761 agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc
     8821 tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac
     8881 tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc
     8941 caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac
     9001 atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac
     9061 attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc
     9121 tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt
     9181 tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga
     9241 tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa
     9301 gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg
     9361 attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt
     9421 ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg
     9481 attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt
     9541 aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag
     9601 gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc
     9661 tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag
     9721 gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt
     9781 tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga
     9841 actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc
     9901 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg
     9961 tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt
    10021 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct
    10081 taatactcga aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt
    10141 gcatctgcgg taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg
    10201 ttcttattac ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc
    10261 agctaacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
    10321 tttctctaat actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga
    10381 agtagcttct tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
    10441 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc
    10501 ttttgcgaag ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga
    10561 tcctgtgtga ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct
    10621 tgacggtctg cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc
    10681 gtatacttga cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact
    10741 gtactcaact caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca
    10801 tgatcagcga aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca
    10861 tttaagatgt cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct
    10921 agacaaactt ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa
    10981 gctgctagaa tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg
    11041 gtcgcttcca agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag
    11101 tcctatatta tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc
    11161 caaattatct gacacagccc tcactataaa actgttctca tatagctaga tgttcattga
    11221 ttcaagaact gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct
    11281 ctgttgtcat cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt
    11341 cttggccagg agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctacca
    11401 attcacgcat atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt
    11461 cgattccatt ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat
    11521 gaggagagct gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg
    11581 ctcctgaacc ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa
    11641 gcatcatatg ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat
    11701 gaggagaaat aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat
    11761 tttcgatcgt attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg
    11821 gtttcaagaa ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca
    11881 tggtggattc tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg
    11941 gatgagaata gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg
    12001 aaatcaagtt gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt
    12061 cagtttcagg gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt
    12121 tagggacacc gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt
    12181 gtttttctgt taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag
    12241 ctttccggtt attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat
    12301 aatcatctag agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa
    12361 tgttgtctgc atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt
    12421 taacatctgt attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca
    12481 aaacttttga cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg
    12541 ttgattcttg gtctgctact tgatctacag atacacctag atggaattcg attcccgcat
    12601 ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag
    12661 aatgggcatc aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca
    12721 agccaatata tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg
    12781 cttgcgtaga atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg
    12841 gttcagaacc agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat
    12901 tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag
    12961 ttgcctcatc gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt
    13021 tccgagcaac agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag
    13081 aaggaataca gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga
    13141 ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa
    13201 aaccgatcac aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac
    13261 cgattggtac aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt
    13321 tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct
    13381 ttattcttct aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa
    13441 gggcagaatt catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg
    13501 atatgcactt ctaatactac accgatctga tagccgccat catctaaatt ataaagagag
    13561 acacgtgctt ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc
    13621 gccccattga aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct
    13681 gcatggattc ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct
    13741 ccattgcgcc cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc
    13801 ctttcgattg accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt
    13861 ctacaaaaat tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa
    13921 caaaaaaata ctactcgtga
//
LOCUS       DAYYAR010000073        13919 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000073 DAYYAR010000000
VERSION     DAYYAR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.073"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..176)
                     /locus_tag="ACHAT0_001801"
     CDS             complement(<1..176)
                     /locus_tag="ACHAT0_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8451735.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLA"
     gene            complement(655..1374)
                     /locus_tag="ACHAT0_001802"
     CDS             complement(655..1374)
                     /locus_tag="ACHAT0_001802"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8451736.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1423..3069)
                     /locus_tag="ACHAT0_001803"
     CDS             complement(1423..3069)
                     /locus_tag="ACHAT0_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM8451737.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3248..3838
                     /locus_tag="ACHAT0_001804"
     CDS             3248..3838
                     /locus_tag="ACHAT0_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8451738.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3930..5435
                     /locus_tag="ACHAT0_001805"
     CDS             3930..5435
                     /locus_tag="ACHAT0_001805"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8451739.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5519..6871)
                     /gene="celB"
                     /locus_tag="ACHAT0_001806"
     CDS             complement(5519..6871)
                     /gene="celB"
                     /locus_tag="ACHAT0_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8451740.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6871..7389)
                     /locus_tag="ACHAT0_001807"
     CDS             complement(6871..7389)
                     /locus_tag="ACHAT0_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451741.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7405..7731)
                     /locus_tag="ACHAT0_001808"
     CDS             complement(7405..7731)
                     /locus_tag="ACHAT0_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8451742.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7744..9723)
                     /locus_tag="ACHAT0_001809"
     CDS             complement(7744..9723)
                     /locus_tag="ACHAT0_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8451743.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9744..10058)
                     /locus_tag="ACHAT0_001810"
     CDS             complement(9744..10058)
                     /locus_tag="ACHAT0_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8451744.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10226..10519
                     /locus_tag="ACHAT0_001811"
     CDS             10226..10519
                     /locus_tag="ACHAT0_001811"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8451745.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10597..11709)
                     /locus_tag="ACHAT0_001812"
     CDS             complement(10597..11709)
                     /locus_tag="ACHAT0_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8451746.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11862..12710
                     /locus_tag="ACHAT0_001813"
                     /pseudo
     CDS             11862..12710
                     /locus_tag="ACHAT0_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13063..>13919)
                     /locus_tag="ACHAT0_001814"
     CDS             complement(13063..>13919)
                     /locus_tag="ACHAT0_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8451747.1"
                     /translation="GLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQL
                     VETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAV
                     FELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKE
                     QEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISI
                     KFRKKRNI"
BASE COUNT         4339 a         2844 c         2386 g         4350 t
ORIGIN      
        1 ctagccaaga acccatctgc tgaaatatga aaatttttga atgctacgac attcgccatt
       61 acaggtatgt aaaagaaaaa aatcatccaa agcatacccg gtacagccat caggacaagt
      121 gctttataac gccatagatt cataaaaaaa tgattgaacc cctttcgttt tttcatctaa
      181 aaaccccttt cgattcattc gcaacgtaat tatagcgctt tcaataatga gaataaataa
      241 acaaagtata gggaaaatga aacaaagtat aggagaggtt gtgaaatcag cgttttggcc
      301 tgagtattag agaaaaaaac gggaaaatag ttcctcatat tttgccgttt tttgagctaa
      361 taccgagggt cagctgattg aacaccgttt atcagaggtt gtgaattcag cgttttggcc
      421 tgagtattag ggagatcgtg tccttactat ctaactctga gtcacaagga acaatgggga
      481 ctgtaacaaa agtcttgtca cagtccctta ttccgaacaa acggtgttcc agaagtagct
      541 tctacggaaa taagccgaaa tttcacaaaa aagccatccg aaagtcagac ggacaagtct
      601 gcttttcaga tggcgctaca actactctgc ttattctttt agctatccat tcaattattt
      661 ttctctcact tgttctagct cttctttggt catgggtcga tattgccctt tattcaaatc
      721 tttaggcagc caaaattttc ccatacggat tctttttaaa tataagactt cttttcctac
      781 tgctttgacc attcgcttaa cttggtgaaa ctttccttca tgcaaaatca gacggataat
      841 tgaagtttct gtctcttgat ctacttgatc aatttggagt tttgccggca atgtctgctc
      901 atcgccatca ataatcagtc cttctgcaaa taaacggata tcttcttctg tcattatacc
      961 gctaacctca gctagatatt ctttctccac atgtttttta ggtgatagta actgatgggc
     1021 aaacaccccg tcattcgtta aaatcaagag gccctctgtg tctttatcta atcggccgac
     1081 tggaaaaaga tcttcccgat aatcctggtc ttccaataaa tcgattacgg tttgatcatg
     1141 attatcctga gtagcagaaa tgactcctgc aggtttatgc aaaatgtagt aaaaatcttt
     1201 ttgatacgta atgatttctc ctaaataagt aaccgcatct tcgaattcat ttacttgcgc
     1261 tttatcgctt ttcaccactt gtccattgac ttcgatcgtt ccttttttga ttaatccttt
     1321 gacttctttg cggctgccaa gtcccatatc tgctaaaaac ttatctaaac gcatgcgttt
     1381 cccccatttc aaaagagcat caatctagct cttctgattc acttatttta tttttaattt
     1441 atgtcgtaaa cgcgctattc ctggtcctag tagtctttct gccaagcgga ttttcaaagc
     1501 taaataacca tacactaaag cccctatacc tgctactaca agaatcaaca atagagactg
     1561 gaatttgcta tcttcgttta aaaaagcacc aaaaatcatc tttgtgatcc acgaagccaa
     1621 aagcataatc aaggtaagaa taaagatcag caatgtcctg cgccagacaa aacttggttt
     1681 aaagcgggct acttcatgga ttctctttaa gatcaaggta caagacacag caaatccgat
     1741 cattgtcgcc aataaagggc cgtatacttc aaataaacga atggctgggt attgaagaag
     1801 gagtttcact agaaacccaa taaccaaata attaattgcc gccttgtttt caaacattcc
     1861 ttgcagcatg ttggaaacta gcatatatag tcctaggaag agtcctacaa aacttgcttg
     1921 gatcagtaca ttacttccta gagaatccgg tgtataaaat aatgtattta gcggataggc
     1981 aagtaacata acaccaaatg tcgccggaaa cataaaaaat gaaaacaact gtaaatttcc
     2041 gcttgtcaat tttgccagac ctactcgatc tttcaacgta acagcttctg taatcaacgg
     2101 taacccagta gacgcgatcg atgttgccaa tgcaataact accatcgtca acttatctgg
     2161 attggcacta aaaatcgaaa aaagatccag taactgtgca ttggaatact cggtggatgc
     2221 agacatgatc ttcataaaag tgaactgatc gactaatttg aagatcgtta cccctgaacc
     2281 cacaataata aacggaatcg cttctttaac ggtatctaac aacaattcct tcgttgcaat
     2341 atttattttg ttttcgctgt agtgaataaa cgccgatgta tacgcttgat gtttcttcaa
     2401 aaaatacaaa aggacaagaa tacttgctag cataccgata aatgccgcga aagttgattg
     2461 cgtgaccgcc gtcacatagt caccatccat gactttcatg atgatgaatg tagccaatag
     2521 catataaaaa atacgtgcca cctgttctac aatctgcgac agcgcataag gcatcatttc
     2581 ttgattgcct tgcaaatacc ctcgtatcac actcatacaa ggaaaaacca atacagcgat
     2641 actcaaagaa cgcatcgttg gaatcagttc ttctccgcca ccagaagcat gagctagcca
     2701 aggagaggcg atgtacatga acaaagcaaa caacaggcct aacccgccca tcatctgtaa
     2761 tgcacggata aataatcgtc gtgaagtgcc atattcattc aatgaattat agcgtgcagt
     2821 ttgttttgca atcgcagcag gtattcctgc tgtagagatc agtagaaaca atgcatatat
     2881 attgtatccc atattgaata atccattggc agccttagca ttttcgccca tccaagcata
     2941 ccagggaatg atatagacag cgcctagcaa tcttgaaatg atattactgg cggtcatcca
     3001 agcagagcct cgtgccattt tttcttgcgg tgttaattga tctaatgctg gttttctttg
     3061 attatccatc ggtcagattc caactttcta caaaacatta ctatcatttt aattggtaaa
     3121 acagaaactg gcaatcgatt ttatataact tttataagaa caattgtttt tttgaattcg
     3181 gtttcgtttt tttggtaaag taaagaaata tacagccttt tatggctaac atttatagga
     3241 gatatcaatg acacaagaaa taaaaaaact acccgttata tttttaggtc ttttctttat
     3301 cacagttgga ttgaattggt tcttgctccc tcacgatata gcttccgccg gagtgggcgc
     3361 catcggacat ttagttgaaa cggccttttc tatccaacgc tcagttactg tttggagcat
     3421 caatcttatg atgctgcttc tagccgctgt cctattagga aaatcagtat tttttaagac
     3481 tgtagcggga agtctgcttt ttcctgttgt tttagagatt gttccaaaag tggaaatgat
     3541 ctcctcacat ttcttttcac tgatttcagg aagtcttttg ttcagtcttg gggtttacac
     3601 attgtacaca gtcggggcct caaatggcgg tataacgata ccgccgatta ttttccacaa
     3661 attttttcgg cttccaatgc cgcaaggact tttacttacc aatagtctta tcgtcttttt
     3721 gaattattat gtttttggat tgttggagac actattcgtt ctcctatcta ttacagtcag
     3781 ttcattgttt atgaaaatac tcatccgctt gtcccctgtg tcaagaaaat ttgaataaag
     3841 ctggattttg tcatagcgga aacaaacgat atattaaaaa gagtgtgcta taataaacca
     3901 agaaattgaa taaagcgagg gatcaatcaa tgagcttctc tttagaagac atccgtcatt
     3961 tactattaaa agaaaatcta ttaaaagaat ttgtttcaac aagaggctgg catcttgatg
     4021 taccagaaaa agctgaattt cttcagttaa gttatgattc aagaaaagct gatgcttcta
     4081 ctttgttttt ctgtaaagga atgaatttca aagaagaata tttggattca gcaatcgaac
     4141 agggtattca atattatgta tcagaacaac catacgaaaa ttcagcagta ggaatcatcg
     4201 taacggatat ccgtaaagca atggcgcttc ttgctatggc tttttacgac tatccgcaaa
     4261 ataaattgaa agtgatcgga ttcactggaa caaaaggtaa aacaactgct gcgtacttta
     4321 caaaagcaat cttggatcac acaacaaata aaaaaacagc attgctttct actatgaata
     4381 caacgttaga tggcaaaaca tatttcaaat cccatttgac aacgcctgag tcattagatt
     4441 tatatcgtat gatgtcagaa gcagttgaaa atggtatgac tcatctagtg atggaagttt
     4501 cttcacaagc ttacaaaaca caacgcgtat acggcttgac tttagatgtc ggaatcttct
     4561 tgaatatctc gcctgaccat atcagtccga tcgaacatcc gacatttgat gactatttct
     4621 attgcaagcg acaattgatc ttgaattcaa aaacagttgt tttaaatcat gaaagtgact
     4681 attttgattt acttagggaa acagccgatc tattccagat tccaacgatc acttatggtc
     4741 gttcggaaga aagcaattat caagtgatcc gttcagataa aggtactcac ggttttactt
     4801 tatcttctca tgaagatcgc ttagctatct gcgatactgc ttatgatatt ttgctggctg
     4861 gaggattcaa ccaagaaaat gcagccagtg cgatcattgc cgcagcacta acaggtgcct
     4921 ccagtcaaga tgcacaagac ggattaaaag aagctcgtgt accaggccga atggatcaat
     4981 tgatccaatc caatggagcg catgtctatg tggactatgc acataattat ctaagcttga
     5041 aaacattgct gaaatttgcc aaaaatgaac atccagatgg ccgtgtaatt gttgtattag
     5101 gcagcccagg aaacaaagca atctcccgtc gacatgattt tggaaaagtg ctatcagaaa
     5161 cagctgacat cgcctttttg acggcagatg acccagcatt tgaagatccg aaaaaaatcg
     5221 cggaagaaat caatgaagca attactaatc cagatttgat cgttcattat gaaatggatc
     5281 gtcctgaagc gattcgacgc gctttagcag aaagcgctcc gcaagattca gtcgttattg
     5341 cgggaaaagg cgtggatccc tatcaaaaaa tcaatggtgt agatgaacct tatgaagggg
     5401 attatgcaat cgtgaagcgg ttgattgaag aataatggta tttatatact agtggtttcc
     5461 catttctaag aaaaaaaggg actgtgattt tttgtcacag ttcctttttt cttagaaatc
     5521 attttaactt ttcactagct aattctgctg ccttttctct ttcttcattt aataatttat
     5581 tatcatagaa tttgataaat gggaaccaga taatgaatgc gatgattgca caaatgactg
     5641 ctaacacagc agctttccaa tcaccaccac taccaacaaa tgctccgatc ccgactggtg
     5701 aaggccacgg catctgtgca agcatcggtt tgacgatttc cagccgaatg gcaaaataag
     5761 cgagtgtagc agaagccatc ggagctaaga aaaacggaac cgctaaatag ggattgtaga
     5821 cgattggcat cccgaaaatg attggttcat tgatattgaa taatcctggt acaagtgagg
     5881 cttttcctaa cactttcagc tgatcagatt tcgcgaggaa tgcaataaag atgatcaatc
     5941 ctaaagtcgc acctgatcca ccaacggtta cataagaatt gttgaattct cctgccaacg
     6001 gataatttgc gccttgtgcg ttagctgcca tattagctaa tacgatcggg gtcaagaaag
     6061 aagtgatgat attcgctcca tggatcccta cgatccacaa tgcatggatc aagaaataaa
     6121 tcaccatgac accaagccat gtattcgtca actgcgtaac aaacccaaat gggatagcaa
     6181 tcattttgaa gatatccgtt cctagtgcaa ctaataatcc attgatgatc atcaccacaa
     6241 acgcaatgac aaatgttggg atcagagcag tgaaagaacg agaaactcct ggcggaacaa
     6301 catctggcat ttttatgacc caatttctct tgacacacat acaatacaat tcgaccgcaa
     6361 taatcgacat aataatagct gtaaagattc ctgaagttcc taaacgttcg acaaaagagc
     6421 ccatcctaat accatttact acagtttctg tctcattgat agaagcaatc aagctgattt
     6481 taccatcagt catgatcaat tcagggatac acataaagaa tgcaaaaaca gatagtaatg
     6541 cccccgtgag cggattaaca tttaattttt gttcatctcg ttcaattgac gttaattcat
     6601 aagcaaacac aatggcaaag taaatagata aaatccccat cgtcattgta ttgacgatca
     6661 tgtacaagtc actaactttg aaaaaagttg cattaaaaaa tccttctaaa aatgggaacg
     6721 gtaatggcaa aatatttaaa accaaaaaca tcgagcctac aatcgtgaaa ggaatagaag
     6781 ccatccccgc tgccatgaca gcccgaacga ttttaagttg cgcaattttc cccataggtc
     6841 ccatcaggta tttctgcaaa aaatcaaaca ttagttattc tcctttccta aatataacgc
     6901 tttttcatat tgtttaatac ggatatactg ttggtcttgg ttgagtgaaa ttttctttaa
     6961 acgatactgt ataaatgcac tggcaaaaat accgcttaca acaagaatga aaacaaataa
     7021 ctgtcctcca ttgttatttg ccataaaagg atacaagaat gtaaatcctg aggtaaacaa
     7081 cactggaatc actaataaat taaagacact ctggaacaaa aaataccact ttgtccatgg
     7141 aacgttcgtt tggtgttttc gataaagctt tacttgttct cctactgcag gcaaaatagc
     7201 taaaatcaga atcatcggta aactacttcc taaactttta gctgatagaa gcagaatcat
     7261 ccattgcatg ttcatgaaaa agaaaaaggc agtcaggtaa cgtacgatta gataacgatt
     7321 gaaatacatg tttttcaaac taagttgctt tttttctaat tcgattttcc tttttactgt
     7381 atcctgcaca atgatccttc ctttctattt atttttttga tacaaataat tcatttcctg
     7441 tgcaacttcc aataatgtca tcgtcgtcat cagatgatct tgtgcatgga ccatgataat
     7501 ttccatttct atttttgtcc cgctggcata ttctttcaga agttgagttt gtgactgatg
     7561 cgcatgaaga atctcttcat ttgcttgtcg caacttctct tctacttctt caaacatctc
     7621 tttacgcatc gagcgaaatg cttcatgtat cagtgtccgt gcatttccgc tatggaaaat
     7681 gatatcaaaa gaagtcactt gtagtgtttc actgtttatt ttttcagcca tctgttttcc
     7741 tccttattcg atcagtttga taaatttact gctgaaattc tcaaaaatag gttcagctaa
     7801 gatttcctgc tgtactttta gctggtcaac taacctaacg attgctttag tcacgaccgt
     7861 aattccttca ttttcgacat aagaaggcga aactaaaaaa acaaaatgaa tttcttgata
     7921 ttgatcccac tgcatcccag aaggaatcaa tgcaacggct acctttgttt cgattccaac
     7981 tggaacagcg ggatgtggga ctgccactgt atcactgaat atgatttgtc ccatattttc
     8041 acgttgctgg atctgctttc gcatttctct tatatatcct tcttcttcat ttatgctcag
     8101 cttttgcagc aaatcatgaa gaatttcttc ctttttcggt tcgtacgtat aagtcttgaa
     8161 aaatttttca gacaactgtt cggtgtaaat ctgctttttt tgctgtagag aaaatgctgg
     8221 tatcggtcta tctgaggaat gatagggtat agattctcca actacctttc gtattttacg
     8281 aacatcatct tcattcaaaa aaatactgac atgtaaaact ggaattttga aaaacattgt
     8341 cgaaagatca atagaagaaa taatcaggtc gattcctttt aaagttgttt cattgatctc
     8401 ataatagcct tttacactta ccactgtaat atttttaccg aattcactga ctacgcgatt
     8461 ctttaatagt tgggcactgc cgtaacccgt tgcacagata atcaatgcat gtagtttatg
     8521 cgcagctctt tctttttcca atgccgccat gagatgcaaa gccaaatagg cccattcatc
     8581 ttcattgatg gtatagtttt tcaaagccgg catgttgctg aaatgccttt ttactgcgtc
     8641 aaagactttg ggattttctt tctttatctc ctcagtcaat gggttctcaa tgcgaatacc
     8701 tcggctcaat cgaatcatca tcggtgacaa atgatcgagt agtccattga aaagctggag
     8761 atcctctact aatgaaaaat gaaattcctg agacaatctt ttcagaactt tcgctaattc
     8821 ctttgatagt tcctgatcat tttgattttc tttgtgattc gacttagcca tgagatgtaa
     8881 tgtcaaataa gcaatttctt cttcgggaaa cgatacacct gcgattacct caatacgttg
     8941 aacgatccgc tgcgccactt gatactctac tcgcttagaa acttcttgtt cgatgcctaa
     9001 ttccttgatt tcaaaccctt ctcggagccg tttgatcccc aaagccaagt gtaaagtcag
     9061 attctgtatg ataaaatccg aaacttttaa cttagcttct cttgtttcat cgagaatgat
     9121 aatgactagc tcttcaaaac tgatgtctgg gaaaaaacgg ctttgcccta aatattcttt
     9181 caatgaattt ccataatggt ttccaaaaaa agtatccatg ataaaatgcc gtcgatttct
     9241 ttcttcccct tcgatccatg tcccttgacc atgcttagaa acgattttta gtgaatatgg
     9301 aatcaatcgc tccttgattt cttgcatgtc ctttgtcaaa ctggaacgac tgatataaag
     9361 ctcttctgcc aaatcatcca taaaaagaat ggcatcttcc aaaagcaatt ggttcaatat
     9421 atacttttgt cgatcttcgg actcataaaa tccttgcggt tctctagcat atccaggtac
     9481 tatttcttgc cgactcaaaa acaagtcaaa tgtcagtctt tgtaaaatat gcagttggta
     9541 gccataacct ggtttagcaa tgatcttacc tccgtttttt tctactaact ctttgatccg
     9601 ctgcaagtaa ttacggattg tccgatcaga taataacaat tcagaagcta aacgttgact
     9661 ggtgacatac tccttcttgt gatcaatcaa atatctcaac agcaattttt ccttctgttt
     9721 cattatcatc cactcctatt ccatcatatg ttctcttcga tcacctctgc taacttctgg
     9781 atgcctgtag ggattgggac atacgcttgg aaagggatac taacaacagg ctttccaaca
     9841 gattctccta attttttgaa tttatccaaa aacatcgtcg tttgcgggct gatcagaaat
     9901 aaatcaaaat cactattctt aatcatatta tcgccttctg tagctgaaac tgcatctacg
     9961 agtacctctt ctcttttttg cttaaagaaa tccgttgtct ttgaggccat catcgaggat
    10021 gacattcctg ccgcacaaat aatcaatgct tttttcataa atcaatcact ccttttattt
    10081 ttataaacca agcatatctt gtaagcgttt tctattcaaa atatatattt ccgtagcatt
    10141 gcggaaaaaa tgtgtatcat atcttgaata ataatttctg gcaccgtgat atactagtga
    10201 tagaattttt tcaggagggg atttagtgag tatcacagtg aatattttgt atacaagtac
    10261 aaatggacaa gccagaaaat ttgcagaaga aatggtcgaa agaaacattg tcaaagcgat
    10321 tcgtgcagaa gaaggaaacg agaaatatga ctattacttt ccactagaag atccagaatc
    10381 tcttttactg atcgaccgtt ggaaagatga agaagcaatc gaaaagcatc acaaatccga
    10441 aatgatggct caaataaacg aactccgtaa aaagtataaa ttaaaaatga aagtcgaacg
    10501 tttcactgat ttaccgtaaa aagatacaca tggaatgata gcaatttatc atccatgtgt
    10561 atcttcactt tttccttatt ttttattcga tctcatctaa tgattcaaac cgtgtttttc
    10621 tcttattttt cggataagta tttcagtcac cccaatatac atgattccta aacatacccc
    10681 aaggatattc tcaaaaatgc ggatcgttac tgctcctgga accccgaata aactggcagt
    10741 gattgccaaa gcaccaaaag aattaaaaac tgttttatat ctataggtag aacaaacacc
    10801 taaagctaga ccacctaata taccgaccca gttaaaagga ataaattggc tgataccaat
    10861 aaataaaaca catccaatca atgagccaat gattcggtct actgctcgtt ctttgaaacc
    10921 aatcgacatc aatccatagc tagatacaat agaagaaaat gcaaaccctg cccacatcaa
    10981 tcgctgaaaa gggacatact ctcctgcaac gatcaaaaga ctaatgccaa atgcatagat
    11041 gagcatccaa attttttcct gcgaatacat gcctttcaaa aaaatctctt taaacaagct
    11101 tttgtctttg ttcttttctc gatgttttct gtaaaggaga actgaaaacc aacaaaaaaa
    11161 caaaaataat aagctacttg tttgtgtaaa gaaattcttg ttcaacgaat ctttaggtaa
    11221 cgaataaaca ataaagagat atgaaaagcc gtacaaagaa gcattcccca ttttgggatc
    11281 actagcggtt gccattaata tgctgcttag taaaataaaa tgaatcaaca tgcccataac
    11341 tgaccatttg atcagctgga catacggtgc tactaatagt gagaacatga cgattgctaa
    11401 acctatgatc gaatgggaaa tcttataacc aaagtcaaca aaacgaatac tcatcaacat
    11461 acagaataaa accacagcgt atggtttatg cttcgattca aataatgttg taattataat
    11521 gataaaacaa atggcaaaca atacgatgaa taatgatcgt aaaaacaatg aacaaatata
    11581 catgcacttt tcttttttag aagctgcttc tcgtattttt tgttttaaaa taaacggatc
    11641 tagttgtaga gactgataaa aatccacctg ttgtcgtcct ttctttttac ttttattttt
    11701 tacagtcatc tatgacaggt tgtaaatact atggaacatc atatggcgtt gtcattttct
    11761 tgtcaactat ttttcctatt catgttttta taaaaaaaca tatcgttttc ttgtatgatt
    11821 aaatcaccac aaaataaaca actgagagaa cgatatgctt tatgaatggt tctatcaaaa
    11881 aaattctgag atcaatagac aaaaatttga tgattacaga ggtctcttac gaaaccttac
    11941 agaagaaaaa aacattgatc gttgctgcag ttctcttccc tgctccatgt gtctgtagaa
    12001 attgtggttc tgctgtggta gatggaaata ggaaattaat ggtggtgaaa aacaagaaga
    12061 aggaaaccat tgtccacttt gaataatata atcatatgcc aatggttata cgcctaaaaa
    12121 agcagcgcta tacctgtaaa aactgtcgaa gccattggaa cgctcaaagt tattttatcc
    12181 gtccaaggca ctcgatttca aatcatgtca gacataaaat cacttcttta ctgactgaaa
    12241 aagtgtcgtt attttttatt tcgaaaagtt gttaggtatc tttgacaacc gttattcgca
    12301 cattgaaaga gttgagaagc tatttaccaa agtcttccaa aagaattctg tcttgagtgt
    12361 tgagggttgt gaatttcgtt ctcacgtttc cgttgaggat aaactgagtt ttatttccgc
    12421 ggatggcgag acagaaaaat taatcgagaa aatttaccta cacaaaaaat tcctcgatta
    12481 acaaactatt tccttagctg cactaatcct aaagaaatag aattcttagt tacagacatg
    12541 aacgccacct actttcagct caccaaacat gttctaccaa atgcgaaatt ggtgattgat
    12601 cgattacttg gtttttccac gcctttcaag gaggcgtaac ctttattcac gagttggttg
    12661 aagctttccg agacaaaaga cccgacttgt ttttctcctt attgacaaag agccattaaa
    12721 actagagaaa aaattacaac tgatcgctga ttatttgtag aaagaaaatc aaagtattac
    12781 ccaataaact tgagtcatta ggcaaaaaac actgcgaaac tcaagaatga ttttcgcagt
    12841 gtttttgtat attttttatt aactagtatt actacaaagg tttatttttc acttctcaac
    12901 gcagcacaaa attgtcttta cttatttact ggttgggttc aaccttctct actccgatac
    12961 taatatttgt ttgtataccc cttctaaaat cggtagtttc cattcaattg atgggtacta
    13021 gttgatttta aaaactatgc ttattcaatt ttttatttta gctcatatat ttcgtttctt
    13081 tctaaatttt atactgatga aaacaagtag cacacccaat attgacaata taaactggct
    13141 aatgatggtt tctccagttt ttggaagatg cttatttcca ctgcttgttg gattgtttcc
    13201 tcttatatct gagctatcag caacttttct attctctttt gttaaaaaga tcgccacttc
    13261 ttgttctttt tcgatcttga aagttactgg tgtcgcatcc aattcatacc ccgttggcgc
    13321 ttgagtttct accagctgat atgctcctgg tgctaagcca tctatcgcca gttttccatc
    13381 tgctcctgtg gttagacctg actgcagaac ggttccattg gcatcttgta actcaaaaac
    13441 agctccttgc aggacttctc cgctttggtc atcgatcttt gtcaacacga caccgcctgg
    13501 tgtaagtcga ttttctttcg tcaattctac caccgctgtc tgacttcttt cgatctcaaa
    13561 ctcgattggt gtcgcatcca attcataccc cgttggcgct tgggtttcta ccagctgata
    13621 cactcctggt gctaagccgt caatcgccag ttttccatct gctcctgtgg ttagacctga
    13681 ctgcagaacg gttccattgg catcttgtaa ctcaaaaaca gctccttgca ggacttcccc
    13741 gctttggtca tcgatctttg tcaacacgac accgcctggt gtaagtcggt ttgctttcgt
    13801 caattctacc accgctgtct gacttctttc gatctcaaac tcgatcggtg tcgcatctaa
    13861 ttcatacccc gttggcgctt gagtttctac cagctgatat gctcctggtg ctaaaccgt
//
LOCUS       DAYYAR010000074        13788 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000074 DAYYAR010000000
VERSION     DAYYAR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.074"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(266..1435)
                     /locus_tag="ACHAT0_001815"
     CDS             complement(266..1435)
                     /locus_tag="ACHAT0_001815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8451748.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1639..2814
                     /gene="mltG"
                     /locus_tag="ACHAT0_001816"
     CDS             1639..2814
                     /gene="mltG"
                     /locus_tag="ACHAT0_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8451749.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            2950..3429
                     /gene="greA"
                     /locus_tag="ACHAT0_001817"
     CDS             2950..3429
                     /gene="greA"
                     /locus_tag="ACHAT0_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8451750.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3598..4329
                     /gene="liaF"
                     /locus_tag="ACHAT0_001818"
     CDS             3598..4329
                     /gene="liaF"
                     /locus_tag="ACHAT0_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8451751.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4326..5393
                     /gene="liaS"
                     /locus_tag="ACHAT0_001819"
     CDS             4326..5393
                     /gene="liaS"
                     /locus_tag="ACHAT0_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8451752.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5400..6032
                     /locus_tag="ACHAT0_001820"
     CDS             5400..6032
                     /locus_tag="ACHAT0_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8451753.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6172..6837
                     /locus_tag="ACHAT0_001821"
     CDS             6172..6837
                     /locus_tag="ACHAT0_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8451754.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6865..7158
                     /locus_tag="ACHAT0_001822"
     CDS             6865..7158
                     /locus_tag="ACHAT0_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8451755.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7226..8173)
                     /locus_tag="ACHAT0_001823"
     CDS             complement(7226..8173)
                     /locus_tag="ACHAT0_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8451756.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8351..9154)
                     /locus_tag="ACHAT0_001824"
     CDS             complement(8351..9154)
                     /locus_tag="ACHAT0_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8451757.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9300..10214
                     /locus_tag="ACHAT0_001825"
     CDS             9300..10214
                     /locus_tag="ACHAT0_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8451758.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10262..10732
                     /locus_tag="ACHAT0_001826"
     CDS             10262..10732
                     /locus_tag="ACHAT0_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8451759.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10817..11425
                     /locus_tag="ACHAT0_001827"
     CDS             10817..11425
                     /locus_tag="ACHAT0_001827"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8451760.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11559..11759
                     /locus_tag="ACHAT0_001828"
     CDS             11559..11759
                     /locus_tag="ACHAT0_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8451761.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            12513..13739
                     /locus_tag="ACHAT0_001829"
     CDS             12513..13739
                     /locus_tag="ACHAT0_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8451762.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         4682 a         2107 c         2788 g         4211 t
ORIGIN      
        1 ttgctagcac atattttagc aatctaaaga tactatttta gatttaatag gccctttgtc
       61 aaataatgtt ggtaaagaga aatcttagaa taatttggac agaacagaaa gaagaacacc
      121 tctttctgtt ctgtttattt ggaattaaag tctgtagcaa aaattgattt ttagatttgt
      181 cccacactct ttcctacgtt tagtcgagta ctttttttaa gcaaatgcta ttcaaagaag
      241 tagtatttgc tatcttttca tcatatcagc tatttgccaa cttagtagtt ccttccatca
      301 ggtcatagat ttggtttctt agatcagcag taggcagttt gatagttttg tcagctttta
      361 tattgctttc catgatgata gcatcctgtg aatctttgct aaatatagca gatgtatcaa
      421 aagagtcgat atttccttgc gacaggatat aattatctcc gctatatacc ccaccactat
      481 atggatgtcc attcgcattt tcacgccata atgagaagag taagtctttg gaaactagtt
      541 ttccagataa aacttgtgta aacagcgtat ttaaatcata gacggacata ctgatattac
      601 cagtccctaa ttcgttatgg atctctgttt cagattctgt cagtggttta aagtaatcat
      661 cctctttaga catttgatag gatagtgcat gattggatga atttttgact tggtcataaa
      721 attttgtatg ctgtaatcct aaaggttcga tgatatcatc ggtcaataat ttttcatacg
      781 gttgatttgt taatttgatc aaaattccag tcaatatggt aaagttcaat gaatcataat
      841 gagaggtctg ataatctgta aattttaagt gctgaatcgc aaattgaacg acttcttcat
      901 cagtcatcgg tatggttgga gaagcagttc ggtgcaagcc tgagcgcata tacagcaagt
      961 cacggatagt aatttgctgg ctgccagcaa tttgtggata gaattgagcc agcttagtat
     1021 ctaaagaaag gtgcccacgc tcgacttgtt ttaaaatcaa aagagtagtc aatgcttttt
     1081 gaaccgaacc aatctgataa taaagagaag gatcatttgg ttgttgcgtg agtttattcg
     1141 catagccata gcctttttcc atgatgatgc gattattttt ctttacgtaa attgagccaa
     1201 tataatcatt ttttcttaat agttgatcga ttttttcaac gagctgttgt tcattggtag
     1261 tcagtatcaa ctttttttga gctgattgag catgggcaac tatctgagag tgttcattat
     1321 tgtaaaattg ccatccaaga atagttcctg tagagaacgc actaaggcta aacaacacga
     1381 caacgatcat taaaaatgaa tattttcttt ttttagcatg ttttcctttt ttcataatag
     1441 tttatttagt ctcctttctt ttttacagaa agaataaagc taaaatagct tgtatcttga
     1501 taacatttat tttaatctta tagtaatgga tttactactt acatttttga tagaatattt
     1561 aaataaaatc attcacgcca attcaaatat catgtataat gaataaagtt acgaacttga
     1621 tagtaaggag gagttgtttt gtcaggaaac aaacaacacc caagtaaaga taaaaaagaa
     1681 gccagtaagg cagctacagc agatataatc gacaagcgga gaaatctgcg taaaaaagaa
     1741 gataagattg tgaggaagat cattttagtg attgctctta cattattgat tattggcgga
     1801 tttcttggat ttaccgtgta tcgttatgta gatagcgggt tgaagccttt agataaatca
     1861 gatgaccaat tagtccaagt cgagatcccg agtggctctt ctaacaagca gattggtgag
     1921 attttggaga aggacaatat tattaaaagc gggattgttt tcaattatta tacaaaattt
     1981 aaaaatttaa ctggctttca agctggctat tatcagttag cgcctaatat gactcttgac
     2041 gagattggca aacagttgca agaaggcggt acttctgagc caacaaaagt tgcagatggg
     2101 aaaattgcca ttccagaagg gtacgatatc gatcagattg ctgaacgggt agcgaaggta
     2161 actggtaaag acaagaaaga atttttagat ttagtgaatg atgagacttt ctttaacgag
     2221 ttgcaccaaa aattcccaga actgttggaa agtgcatcta aagctgagaa cacaaaatac
     2281 cgtttggaag gttacttatt cccagccaca tatgattatt acacgagtac ttcgcttaag
     2341 gatctagtga ttgaaatggt gaacaagacg aatacggtca tgcaaaacta ttattcagct
     2401 atcaagcaga aaaatttgac tgtacaagaa gttttaactc tagcttctct agttgaaaaa
     2461 gaaggcgtaa aagaaaatga tcgtaaaaac attgcacaag tcttctttaa ccgaatcaaa
     2521 gcaaatatgc cacttcagtc agatatctct gttttatacg cattaggtga acacaaagaa
     2581 ttagtgacgt atgaagatac agctgtagat tcaccatata atttgtatac caataccggc
     2641 tatggacctg gaccttttga caatccaagc gaagaggcaa tcaaggcagt attagaacca
     2701 gcagaaaatg actattatta tttcgtagca gatacgagca ctggaaatgt ttatttcgct
     2761 aaaacttatg aagaacatat ggaattagtg gaaaaatatg taaataattc ttagaaaaac
     2821 aggctctaga cattgatcat ttttagaaaa acatttacat ttgaagaaaa gcgtggtaat
     2881 cttttttgga ctatattcct caaaagatta ccttttgcgg tattttatcg aaaaaaggag
     2941 acgttacaca tggtagagaa agtttatcca atgactctag aaggtaaaga aaaattagaa
     3001 aaagaattag aagaactaaa aacagtcaaa cgcggagaaa tcattgaacg aatcaaaatt
     3061 gcccgcggtt tcggagatct ttccgaaaac tctgaatatg aatcagcgaa agatgaacaa
     3121 gcatttgttg aaggaagaat cacaacaatt gaaaatatga ttcgttttgc tcaaatcatc
     3181 gataacgacg gtgtagatgc agatgaggta tcaattggta aaacagttac attcatcgaa
     3241 ctaccagatg gagaagaaga agagtacacg attgtcggaa gcgctgaagc agatccgttt
     3301 tctggcaaaa tttccaatga ttctcctatt gcgcaagcat tgattggcaa acgtaaaaat
     3361 gatgaagtaa ctattgctac tccaggtggc gatatgttag ttaaaatcgt caaagtagaa
     3421 caagcgtagt ctgactgaaa gatctagaga gttcatctta ctgatgatac actctagatc
     3481 tttttctctt tgtgtatttt agatttcaac aaacgatctt acatctaaag tcctatagcc
     3541 aacagttaaa aaaataggta tgatgaaatg agaatgtgtc aggagggaga aagagcgatg
     3601 aatagttctt ggcgtttttt tgtggtagtt gaagcactac tgctgttatt tgctgtttgg
     3661 caaatcgtaa acaatactgg acttttgctt ttagtgatat tcggtatttt caatatatat
     3721 ctagctatgc gcaaacatcc tcgtactaaa tttcaaaatt ttcaattgat attaggaagt
     3781 ttggtcattt tttttagctt agtcaacagt cctgctttat ggatgatggc tgtatttgcc
     3841 gttttattta ttggtctgaa aggtgtagag atttcaggga tagatttaac caaaaatgct
     3901 ttttggcgaa agaaacagat catgatgatc caaacagagc aattaaaaac acataataat
     3961 gagcggaaaa gacagcaatt attcggtaac caacgtattg gaaacgacgt ctatgaatgg
     4021 gacgatatca acatcgccgt catttctggt gatactatca tagatttggg caatacgctt
     4081 ttgccaaaag atgataatat tgtgattgta agaaaaggga taggcagaac acggatctta
     4141 gtacctttgg gcgtagccat ccgattagag catgcgacat tagtaggaaa tgtcttgttt
     4201 gaagaagagc aattttcttt gaaaaatgaa caaatcaaaa tctacagcag tgattatgat
     4261 gaaaaccctc gtagattgaa gattattact aataccttga ttggagatgt cgaggtgata
     4321 cgaatatgat gggaaaaata tccagagcga tgctagctgt ttattcgggg attgcggctt
     4381 tccttattat tctattttca cttttcactt atttttatgc cagcaatcaa agtcattggt
     4441 ggggagaatt actgcgtgca cgtttattat atgttccgct tatcttccat ttgttagcca
     4501 tttccttagg tgtaggatta attgtctttc tattattatc acttattcaa aagacaaaat
     4561 acgggaaaat cgaagaaaag ctgcgtgcac tttcttctgg caattatgaa tccaaattgt
     4621 tgcttcttcc gatcccaagt gcatcggacg atttatacat caaagatatt gataaggaaa
     4681 tcactaagat aaaagaaaaa atgattgaag tatctagtga attacaaatt gtaacgagcc
     4741 gtccccaata tgtagatgga caaaccaaag aagaaatttt agaattagaa agacatcgat
     4801 tagcccgtga gctgcatgat tcagtttcgc aacaattgtt tgcagcaatg atgatgatgt
     4861 cggcattgac ggagcaagca gaaaaaagcg agacaccaga aatgtttcgc aagcagttga
     4921 aaatggtagc agagatcatc aacgcttccc agtctgagat gcgtgcgctg cttcttcatc
     4981 tacgtccagt caatttagaa gagaaaagtt taaagcaagg tatcgagcag ttattgaagg
     5041 aattgcaaaa taaaatccag atttcgctga aatgggatgt agaagatgta aaactatcta
     5101 gttccattga ggatcacttg ttccgaatcg ttcaagaatt gttatcaaat acattaagac
     5161 atgctaaagc caatgaatta gaggtatatt tgcacaaaat agacaataat cttttgttac
     5221 gtattattga cgacggaaca gggtttaata tgaatgaaac gaaaacggga agttatggat
     5281 tgaacaatat caaagaacga gtagctggta ttggtggaac agtaaaaatc attagtttta
     5341 aaggtcaagg tacaagtgta gaaatcaagg tccctttgat gaaggaggca tagataagga
     5401 tgataaaagt tttattagta gatgaccatg aaatggtgcg cttaggcgtc tctttttatt
     5461 tatctattca agaagacatt gaagtaattg gagaagctga aaatggacga caaggctatg
     5521 agaaggcgat gacacttcga ccggacgtca ttttaatgga cttagtcatg gaagaaatgg
     5581 atggtatcga atcaacaaaa gcaatcttaa aagattggcc agaagctaaa atcattatcg
     5641 taaccagttt tattgatgac gaaaaagttt atcctgccat tgaagcaggt gcagcagggt
     5701 atttgttgaa gacctctacc gcacatgaaa ttgctgatgc tataagggca acgcaacgtg
     5761 gcgagcgagt attagaacca gaagtgacaa caaaaatgat ggaaaaaatg agtcggcgaa
     5821 atgagcctgt attacatgaa gaactaacga atcgggaaaa tgaaatttta atgctgattt
     5881 ctgaaggaaa aagtaatcag gaaatagcgg acgaattgtt tattacactg aaaacagtaa
     5941 aaacacatgt ttccaatata ttggctaagc tagaagtgga agaccgtaca caagccgcta
     6001 tctatgcatt caaacatggt ttggtgaaat aaaaacgatg ataaagagga taatcaaaag
     6061 ctgagaaaat aactattatt tttcttggct tttttacaat aatgatgctt gcttttgttt
     6121 ttgtaaaggc tttgttatac tgagaaaaga agatactaaa ggaggaaaac ggtgaaacaa
     6181 aattttgcaa ttgtaggtct tggacgattt ggcggaagta tctgccgaac actgatcgaa
     6241 gctggtcaag aagtactggc tattgatagt aatgaagatc gggtcaatga atatatgaat
     6301 atagccactc atgctgttgt ggcaaatgca caggatgaaa tgacacttcg atcattaggc
     6361 ataagaaatt tcgatcatgt aattgtcgct attggagaag atattcaagc cagtatctta
     6421 gttactctga tggtaaagga aatgggcgta ccaaatattt tagcaaaagc ccaaaatgag
     6481 tatcatgcca gagtacttga aaagatcgga gctgatcgcg tcgtccatcc cgagcgagac
     6541 atgggaataa ggattgcgca taatttagtg tcaaagaata ttttagatta cctagagttg
     6601 tcagataaat attctttagc agaaatccga gtctccaatc ctaaattttt caacaaaaca
     6661 ttagctgaat taaatttcag gcaacgtttt gacttaacag tagtcgccat acgaagagta
     6721 gatcaaacgg ttatcgcttc tcctgcagca gatgaatatg ttcgtgaaaa tgataatcta
     6781 ctggttattg gagagacagg cgatgtggat attctagacg ataagatgaa tcaatagaaa
     6841 gaaaagagaa agtaggttgt tgggatgaaa ttaacggtaa cgccaaaagc acaaaagtgg
     6901 tttgactcag aaattgatct gccagaaact tacggaatac gtttttttgg aaaagtctac
     6961 ggcaaaacag aggttcatga tggtttttcg attggaatgt cagttgaaca gccagaacat
     7021 cctgtaaaga aagtggaaat tgatgggaca ctttttttta tcgaggaagc agatgaatgg
     7081 ttctttaaag gatatgattt acttgttgat tatgatccca acctggagga gccaacctat
     7141 cactttaaaa aacaataaaa aaagagaggg tgacacaatt tttgtcacct tctctttttt
     7201 cttttgaaaa tattgttttc agctattaat atcctgcaga caattttact tgattggttt
     7261 gaagctcatg ggtggcaaag tagcttttca gattggttaa aaaaatagcc attaatttct
     7321 tttgaaaatc aggtgtcaaa cctgcaatgt gggaagtaat cagtacattg tccattttcc
     7381 aaagaggatg atcttgtgga agaggctctt cctcaaaaac gtctaatgct gcaaaggaaa
     7441 gttgctggtt atttaaagcc tgcaccagat cttgtgtctt aacagaagca ccacggccaa
     7501 cattgatgaa tacagatttt ggatcaaagt gttcaaacat atgctgatca aagagcccag
     7561 ttgtttcctc cgttagaggt aagatattga ctacgaaatc tacttcagga agaacttcct
     7621 ttagttgatt taaagaaaaa gtcttttcca ttccttctgc tggatgaccg gatgtattga
     7681 ttccatagca gttcatgtct aatgctttgc atttttttgc aatcattttt ccaatatggc
     7741 cagttccaat gattagtatt tttttccctt ggatagattg gtaatggacc gatgaaccta
     7801 accatttcat gtcactctgt gccttttgtg cttgaaaaag accacgagaa tacccaagga
     7861 tgattcctaa gacatgctcc gtaatcgatt cactgtggat gccactacca tttgaaagta
     7921 aaatatgttt ctttgaaaaa gtctcaaggg gtagctgatc aacaccagct gagatagatt
     7981 ggacccattt caaggtagaa gaggctaata gtttttcttg gtagtctgag ttccagccca
     8041 aactgatctc tacattttgt agatcctctg gttgtaattc tgttttaaag atataaccag
     8101 gcgctatagt atggatttct tcgataaatt catcacgaaa tggacgatct gcataaatga
     8161 ttctttctga catagttaat ttcccgcctt tctttactct aagtgtagcg catacaagaa
     8221 gaactttgaa gcgccttgct tgttttttga gaaagaaata tttttcaaat aaaaaagggg
     8281 ctgtgacaaa aaaatgtcac aacctctaaa agggtctatt gaagtagaaa taacgagttg
     8341 tctttatttc ttaattgatc gatatttttg cccatccttt gacagcttga gcaaaaataa
     8401 agattcctgc aaaagcaatg aaggcgctag tgtcaaaaga aggaagaaag accatcaaga
     8461 ttgttgcaat tatagtaggc aatgaaattg aatagatcaa tgtttttaaa caatcaagaa
     8521 aagtagcttt tgttagtcga aagcgtgcat agagaaaagc agcaatgttg gtaaatagca
     8581 aagtgatcag cagattcaga aaactaggat aaatagaaaa tataaatgtc aggactttta
     8641 tccaaagtgg tatagatgct tcacttaaaa aagaacggag ctgcttacct gttaagtttt
     8701 ttaaagattc attggtgtag ggaagttcta attggttatt gttcaacaaa gcagaagtcg
     8761 tgcctgtatt aggaagagct atgataagtt tgttttttaa catcccgaca ctaacaaaat
     8821 tccccatcaa atcagtagag atgtcctgtt ctgttcgttt tccctctgga tcaaatgtga
     8881 agataatgga gtttgtttgg taaatgaatc cttctttttc ttcggtttga agctgcccgt
     8941 tttggattgt aaaatcagga atgcgagtag caatcttttg cccatccaat tgaatgttgt
     9001 ttaagacttg aaaaacttga aaagagattg gcaaagccat aattatactc aaaactaaaa
     9061 gatagacaat ggctttccaa aatgggacat ttctcgcctc ctttaggtct ttgaatcgta
     9121 taaaggaact gataattaat tgttttgttg tcataagtac tcctcatcta gtagtgtcat
     9181 cacttgcaat tctagcgaag tatcaagtga aatacaagtg ctttaaggaa acagctgaat
     9241 gattggtgaa atatcaaaaa aatgttattc ttgtctaaag actatccaat ataaataaaa
     9301 tggaggaagg aatagtgcct acacagccac agagatcatc aaaaaaacta tttggagtag
     9361 tggttttgaa tgcggtgatc actttatcag aatttattgg agggattatg tcagggagtt
     9421 tgtctcttgt ttcagatgct ttccataacc ttagtgatac attggcaatt attttttctt
     9481 acggggcaca aaaaatggcc cgaaaagaag caaataaaaa acgtacgtat ggctaccagc
     9541 gtttagaaat attgagtgct ttcatcaact cattcatatt gatcatatta tccttgtttt
     9601 taaccgttga agcattcaag cggttcactt caccagaaaa gataaatagt catttgatgc
     9661 taacagtagc agttattgga ttgctcgcga atttgttctc agtattatta ttacgacaag
     9721 aagcagatga aagtttgaat ataaaatcta gttatttgca tttgttaagt gatacactgt
     9781 cttcggtaag tgtgattatc ggagcaattt tgatccgttt tttcgatatc tactggatcg
     9841 atccagttat tacactgatc atttcgatat atatattgat agaagcgata atggtgatca
     9901 aaaaagctgc cgctattttg atacaaagtg cacctgctat cgactatgaa aaaatggaac
     9961 aagaaattga agcaatcgat ggggtaaagg atgttcatca tgtccatatt tggcagtata
    10021 gcgaaaagat aattattttc gacggacata ttgattttga agatcagcta ctaagcgaga
    10081 ttgaaaagat atatcctcgt ataatttctt tattaaaatt aaaatatggt attacacatg
    10141 tgacgatcca agcagagaca aaggcaaagg atcaaaaaaa attaattttt ttaaataaag
    10201 atgaaagtta ttgaaatata gagtatgata aataagaatt tttaataagg taggttggct
    10261 catgaagcag tatcgacagt taaaaggata tttagaagca aaaggatatt gggagatact
    10321 agtctatctc ttttttggtg gtttggctac gattgtaaat tttgttagtt ttgctctcgc
    10381 acgccaatat tttgatttaa gtatggcctt atccaattct atttcgtggt tttgttctgt
    10441 attgtttgct tttgtaacga acaaactttg ggtattccat tcaaaatcac cgaattttac
    10501 acatgcactt attgaatgcg gtaagttctt cttctaccgt atcctttctt acggattgga
    10561 catgggggca atggtattgt tgatcaatgt tatgaacagc aatgagtatg tggcgaaaat
    10621 catcactcaa attattgtta tccttgcgaa ctatattttc agcaagttgt ttatttttaa
    10681 agaaactgag gtttttgaag aagaagtagg tcaaagtgaa aaagacgact aggcataaca
    10741 attgaagaat ttccttctct ttgttactct ttgtaatgta gacattatta agaaaacatt
    10801 gaggaggaat ttttttatga cttacacatt accagatcta ccttatgcat atgacgcatt
    10861 ggaaccttat attgacgaag aaacgatgca tctgcatcat gataagcatc acaatactta
    10921 tgtgacgaat ttaaattcag caattgagaa atacccagaa ttaggcgaaa aaacaataga
    10981 agaattatta tctgatatgg acgctattcc aacagatatc aagacagctg tacgtaacaa
    11041 tggtggcgga catgctaacc attcattttt ctgggaaatc atggcaccaa atgctggtgg
    11101 cgaacctaca ggagaaataa aagaagcgat taatgaagct tttggtgatt tttcttcttt
    11161 taaagaagaa ttcaaaaaag cagccgctgg acgatttggt tctggatggg cttggcttgt
    11221 aatggaaaat ggaaaattag ctattacctc tactgcaaat caagattctc cattgatgga
    11281 aggaaagaca ccaattctag gtttggacgt ttgggaacat gcttattatt tgaaatataa
    11341 aaatgtccga ccagattata ttgcagcatt ctggaatgtt attaattggg atgaagtaaa
    11401 caaacgtttc gaagcagcaa aataaccatt tcctccgtca tatcagtctt aaaaataatt
    11461 tggaaaataa aagagcgaaa gcttgctagc gttttctaaa ttgggtataa tgttcttgca
    11521 aggttacttg caagaacttt tttggaggat atacatttat ggaacacgga acggttaaat
    11581 ggttcaacaa tgaaaaagga tttgggttta ttacagtaga cggcggagat gacgtatttg
    11641 ttcatttctc agcaattcaa ggagacggct tcaagagcct agaagaaggt caagaagtag
    11701 aattcacgat tgttgaaggt gcacgcggac ctcaagctgc tgaagtgact aaattataat
    11761 aatttgttga ataaagacac gacttcttag agagtcgtgt ctttttgtct atttatgaag
    11821 tttttagatt ttttggctaa gtaaatgcat ttgttcactt gacatgtcct aactaattat
    11881 caagcaagga acattttctt cgttagtaat ggattttaaa gaaatttcag tttttggatg
    11941 ttgaataaaa gaccatttgt gaaaccacta gtatacatac tcattaccat gtcaattcta
    12001 ttactttaaa agaaaatcgt tttactttga agaggagcat atctatccat taaaagatga
    12061 tggtaatgca atcactagtt cgagtcattt taagagccat gcagtgagct ggaaataaaa
    12121 cgaatagaag atgggtttaa caaaaggacc agtcatagga agaaattgtc gtgaaatcga
    12181 atgataatct gaacggtagg caaagactgt taaaaagatg ggaaaggtat gtgcgatcat
    12241 tttactgcac gattatttgg aaaatacctt gttgttcgct ttttctggaa aaaatacata
    12301 aaaaaagata aaacgattgc gaactgcgtg tattcggtct ttcgatcttt ttcacggtat
    12361 ggtatgatag ttaagaattt tgagaaattt taaaaaataa taatcgttac acaaaaatgt
    12421 tttttaaagt tttcttatga ttaaaaagtg aatttgctga ttgaacagtt atatgagaca
    12481 atattttaga aaatgtgaaa cagaggtgta ttatgaagcg atggcaaaaa gttctattaa
    12541 ccttgttagg ggtattcgtt ttgctgattg ccggattatc agcatacgga atcagattta
    12601 tgggagaagc gagccaaaca gtaaacaaaa tttctaaaaa ctctaatcga atatcctcca
    12661 aacgttctga gagagtaagc attgatgaca aagaaccttt ttcggttttg ctattaggtt
    12721 tagataccgg tggattagga agaacggaac aaggacgttc tgatacgatg atggtagtaa
    12781 cagttaatcc gaaacaaaaa aaatcaacga tcatcagttt ggaccgagat atttatacga
    12841 atatcgtagg ttatggaacg gtagataaat taaatcatgc ctacgcattt ggtggtgtcg
    12901 aaatggccat ggattcaatt gagcaactgc tcgatattcc tattgatcat tatgtaacga
    12961 tcaatttgga tggcatggaa gatttgatca atgcggtcgg tggcatcaaa gtgaataata
    13021 aaattgactt tactttggat ggtgtccatg taccagtagg tgaacaaaca ttgaatggag
    13081 aaaaaggttt agcctattct cgaatgcgac acgaagatcc agaaggcgat atcggtcgac
    13141 aacgtcgtca gcgggaagta gtgacgaaaa tcgtcaataa agttttaagt ttagatggcg
    13201 taagcaatta tagaaaaatt ttaaaagcag ttgagaacaa tgtgacgacg gacttagatt
    13261 gggatgacat gcttgatgta gcgacgaact acaccccggc gtttgaaaca atcaaacaag
    13321 accagttgca aggcgaagga caaatgatca atgatatcta ctatcaaatt ttaggaaaaa
    13381 atgatcttct ttcgatccaa aatgaactga agaaacaatt aaatataaaa acaagcgata
    13441 cattacctaa tttgaaaaat gagaatgctt caatgatgtt ctttgatgat tcggaagatg
    13501 gcgattcgac aaacggtgat ggatcgaata gtgaagggta caccgattat tcttcctatt
    13561 cagaaaacac atatgatcag taccaagatc cgaataccta ttatcagcaa acagaagata
    13621 caagtcagta ctatacacca agtcaagata ctcaaagtta tgatccttac gctggacagg
    13681 aacaaaatgg ttatggttat gaccagaata atgagtatgg aacaacaagt gggtactaga
    13741 aaaaaatagt ttggttaata aagagtgcca cctaaattag acaaaaaa
//
LOCUS       DAYYAR010000075        13698 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000075 DAYYAR010000000
VERSION     DAYYAR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.075"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..675
                     /locus_tag="ACHAT0_001830"
     CDS             <1..675
                     /locus_tag="ACHAT0_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8451763.1"
                     /translation="VLSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFL
                     LSFAEKVYANSSIIIVSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHN
                     VEALGYAVFIPVFFVSVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFS
                     QHSSLMVGAGMISRGEMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFT
                     KKVYEV"
     gene            complement(784..2823)
                     /locus_tag="ACHAT0_001831"
     CDS             complement(784..2823)
                     /locus_tag="ACHAT0_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8451764.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2881..3438)
                     /locus_tag="ACHAT0_001832"
                     /pseudo
     CDS             complement(2881..3438)
                     /locus_tag="ACHAT0_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(3586..3752)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(3977..4183)
                     /locus_tag="ACHAT0_001833"
     CDS             complement(3977..4183)
                     /locus_tag="ACHAT0_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451765.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(4199..4813)
                     /locus_tag="ACHAT0_001834"
     CDS             complement(4199..4813)
                     /locus_tag="ACHAT0_001834"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8451766.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(4960..5643)
                     /locus_tag="ACHAT0_001835"
     CDS             complement(4960..5643)
                     /locus_tag="ACHAT0_001835"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8451767.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(5752..6210)
                     /locus_tag="ACHAT0_001836"
     CDS             complement(5752..6210)
                     /locus_tag="ACHAT0_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8451768.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(6294..8057)
                     /locus_tag="ACHAT0_001837"
     CDS             complement(6294..8057)
                     /locus_tag="ACHAT0_001837"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8451769.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(8278..9738)
                     /locus_tag="ACHAT0_001838"
     CDS             complement(8278..9738)
                     /locus_tag="ACHAT0_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451770.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(9946..10872)
                     /gene="glsA"
                     /locus_tag="ACHAT0_001839"
     CDS             complement(9946..10872)
                     /gene="glsA"
                     /locus_tag="ACHAT0_001839"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8451771.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(10895..12322)
                     /locus_tag="ACHAT0_001840"
     CDS             complement(10895..12322)
                     /locus_tag="ACHAT0_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8451772.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(12353..13567)
                     /locus_tag="ACHAT0_001841"
     CDS             complement(12353..13567)
                     /locus_tag="ACHAT0_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8451773.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3915 a         2825 c         2319 g         4639 t
ORIGIN      
        1 gttctaagtt tcagcctatc ttttctaacg ggggaagctt ctagcaatgc ttctttaccg
       61 cttgttttga ttgaacagct aatttatttt gtttttattt tcttattagt caaatggatt
      121 gcgcctttcc tactttcatt tgcagaaaaa gtgtacgcaa attcttctat tatcatcgta
      181 tcgctagtta tttgtttagg aatgtcttac gtagcggatc ttgttgggct tagctctgtc
      241 attggtgctt tttttgctgg gatagctgtc agtcagacaa aggttaaaga agaagtatac
      301 cacaatgtag aggctttagg atatgcagta tttataccag tattttttgt cagtgtagga
      361 ttagaagttg atttttctga ctttagtgaa caactactct ttatttctat attgacgctt
      421 gtagcgattt tgaccaaatt gcttggaggt tattttggtg ctcagattgc cggtttttct
      481 caacatagtt cattgatggt cggtgctggt atgatttcgc gtggagaaat ggctttgatc
      541 attttacaaa ttggacagca aagtcaattg atcgaaccac agtactattc acctttagtt
      601 atcgtgattt tattaagtac acttatttct ccacttgttc tcaaatattt tacaaaaaaa
      661 gtttatgaag tgtgagtaaa tcaaaaagaa gttgtgaaag agacatcgtg aaaaccgttt
      721 atttgaaaaa aggactgtga cgataacgcc acagtccttt tactgtactt aaaaagcaat
      781 tttttattga taattttggt tgaggtattg caatagttcc gatgctcgtt tggttgctga
      841 atcatcatct tctttatttt ccaacatgct gaccatcata taaccttggt tatcaagatt
      901 gaaagcaaac aagaaactgt tctcttgccc ctttacatcc tgtttttctt tgatttccgc
      961 tgtaccagtt ttcgctgcca acggaatccc taaagcagaa agagaatgtg ctgtaccatt
     1021 tacatcttgc acaacttctc tcagatctgg cacgatcgtt tgtactgctg tttcgctgat
     1081 gacattcttc ttatcttttg tctctttatc tgcaatcaat ttaggataga caagtgtgcc
     1141 attgttggta aaaacagaat acattgctgc ttgctggata ggagtaatta gaagttcgcc
     1201 ctgtccatat ccagtatcag ctagcaagat atctgagtta aagctatctt cattagaaat
     1261 ttgtgctgga ttcatactaa ttggcaaatc aaggtcttca ccaaaaatga atttatctaa
     1321 gcctgtacga aattttttct cacccatttt caacgtttct tgtgccgcat atatattatc
     1381 ggaatagatc aaagcagttt ttaagtctac ttgtgataca tcactactga cacgcgttgt
     1441 tttaaaagat ccccaagaac tgtctttttg ccatttaagc ccgttgatcg tcaacacttc
     1501 atttggatcg atagtgccgt tgtcgagacc gattgctgct gtaatcattt taaacgtaga
     1561 tccaggagca taacctgtcg caaatcggct gatgaatgga tgttcaggat tttcttcata
     1621 agctttgtaa tcttcttgtg agatcccgtt tgtcattttg tttggatcat agcttggaga
     1681 gctagcaagc gcaagaagat caccggtttt tggcgttgtc gcaacagttg atgcagcttt
     1741 tcctcctaga ctgtcaaaag ctattttttg tgccttcgca tcgattgtca atttgatatc
     1801 tttgccattt tgaacttcat gttcgatcag aacttttttc tctacaccgt ctgcatctgt
     1861 aatgcttaat ttcccacctg ttgtcccacg aagatcctta tcaaaagcca tctccaaacc
     1921 tgagcgaccg atttttccat tactgcttaa ttctggattc ttatcaatat cttctgctgt
     1981 gatatctccc acgtaaccaa tcagctgagc agctgcttca cccaaaggat aatatctgcc
     2041 gtctacttct tgaatggtag ctccagctgg aagttctggc gttgctccat caatgatttt
     2101 caatgggaca aagtaatcgg gttgtaccca gctttgtgaa atcgcctgat tgatagcatc
     2161 ttctgttaag tcgaaggagg aagcaatcgc tttgatattg gctgtttttt cgcctccatc
     2221 tccaagttta cttggaacga ctcctaattg ttttagtttg cctgttgttg ctaatggttc
     2281 accatttcga tctataatat tccctcttgc tgcttcttgt gtggtcaggc ttactttgtc
     2341 atttccttcc atttctggaa aaaccaagtt gggttgccag ttgatcgtgg tttgaccatc
     2401 atttctgtct aatgttcctt tataagaaag atctttcaat tcacctaatg aggtattcat
     2461 ctttgcttta tatgaaaaac tgtaagtaga atcgtctttt ttatctactt ttagattcga
     2521 tatctgaagt cctttgacat cagcagcacc gtatatattc tggtatttat ctaagatctc
     2581 tttttcagat aatgcacttt tatttgccgc cttttttgat gtcatttctg cagctttgtt
     2641 ataatctccc ttgttcaaag cttggacaaa ttgctcaact gtcttttctc cagcttctac
     2701 tgcttgggtt tcttgatagt gctggtagta aaaataaccg ccacccgcag ctgctattaa
     2761 gatcactgcg ccggcaatat aagcgcctgt tcgatttttg ccgtgcttgt cacttctttt
     2821 cattttttcc tccttctctt gcttgtcatt cctttatttc actatttata cctgtttatt
     2881 ctaacatatg tctttgagga aatccatacc acaatccaga agcacacttt ctggaaggtt
     2941 cgtttggata ctaccaagta atttcccaac cgctttgggt agcttgaata ttgaagttac
     3001 gtctttcagc tgctgagaga tagctttgat cacttgctgc tgccttctga tcctcccgaa
     3061 gtctccttct ttgtcttccc gaaatcgagc atactggagt aaagtattac catccatcac
     3121 ttgctctcct ttgtttatat atacgccatc taaatctaga gatttttctg catcgatctt
     3181 gactccacca ggaaacagtt cattaacgca atcgataaaa tcttgaaatg tgatacttgc
     3241 ataataggga gcttcaaatt tgaaattttc ctttaatgtt tgatttaata aatcaactcc
     3301 cccataagag taagctgcat ttatcttatt cattccgtaa cctggaatat cgacatatga
     3361 atctctcatg atagagatca gttttgcttg ttgtgttttc tggttgtatt gtgcaaccag
     3421 aagtgaatcc gagcgacctc caccaatcga tcttttttca ccgcatctcc tcctcacaca
     3481 gcaatccact tgtcaggtga ggagcgagtt tgtctcatca aataagtcag tcacaaaaat
     3541 ataaagaaaa ggtcagaaac cttttcgaac aagaattctg acctagcatc atctcatcgt
     3601 ccgagtttta gcactataca acgtaaagaa aacattctta gctattggaa aaagccttaa
     3661 tccaacagtt tctgttttac ccattggcga ctttcgacgt ttctgggcaa cagcctatgt
     3721 ttgtatagga gcctcaccta acaaggttat tactatattc tatttacgag ttcatgatag
     3781 acttttccta atgattaggc aatggaattt gaaatgaaat agtgaaattt aacaatcttt
     3841 aatatttttt agaaaaaaat aacaaataag ttgaaagaat cccgttgcaa attctttcag
     3901 ttgcaccagg attctttgat aattttttag ttttatgtgt ttttttatat gttttttcac
     3961 ttttcggaat aaatatttat ttgttaaagt aattaaacaa tccgctaatc gtttgatctg
     4021 gcatttctat ttccttacca tctaaagtaa cagtagtaac gacaaaatgg tcttgcaatg
     4081 gcggaacttc taagtactgc ccttgcagaa aatcattcac tgtttcatat tcttttttca
     4141 tttcttttga atctgtcgat ggtttatagc ttatttttaa cataactagt gctccttttt
     4201 attcgggacg tgcaatcgtg aaaatttctc ctattgctgc ataatcattt cctgccaaac
     4261 ggcttacagc attcaatttt cttggatcga tccttcctgt cttatcatca tagactgttt
     4321 catttaaatg atatttctgt acttctagca aaaataaatc agctgttact ttatttttat
     4381 tccgtatctc gacatgttca tataaggatg tttcaaaacg aacttttgct tctgtcaatg
     4441 ctggtactga aatgattact gaatcagtta aagtcaagtc agtcaggtac agttcgcttt
     4501 cctcattagg cagattggct gctgttttat ttacttcttc tacgttgtcc tcatcaagga
     4561 tatggataac agcttctctt gtctcaagaa tattctttgc tgtgtctttt actgcatctt
     4621 gttttctctg gatcgccaag gataagatgg gtcgattcga actgacgata ttgaagtaac
     4681 tgaagggtgc gatattcact atatcttttg ctgatttagt cgttactact gctactggtc
     4741 tagggatgac tgaaccaatc agcaacttgt aattttcacg ttctgtcaat tctgaaggac
     4801 tccgttcaaa catggtttcg ccttctttct ataaaattcg ttaaaaagca gatcttgaag
     4861 acaaaatgaa aagtaatgct tttttcatct tcttagaatc tgctttaaac tatctatcaa
     4921 tttttctttt ttgtcaagaa tctgtgccgt gcgtgttttt tagttatttg ctttacttgc
     4981 ggcttcgaca tatcttttag tgatcaattg cggacgggtg ataatagatc caactacgac
     5041 tgaaaaagca cctaattcat atgctttctc tacttgttca ggagagtcag tatgtccttc
     5101 cataattact gggatatcga cttgttctat gatttgattc aataaatcaa aatcaggtgt
     5161 ctgcgtcttt cgtgaatatt ctgtatagcc tgctaaagtc gtagacaaaa gatcaaaacc
     5221 atcttcttgc gcttgaagcg cttcctcaaa agtcgaaata tctgccatag ctaaccgacc
     5281 gtgttcgtga atcatttgga caagttcagc agttgttttt tctgtcggac gtttacgaga
     5341 agttgcatcc agtgcaatga tttctgcttg cgtctccaaa agttcttcca cttctttttc
     5401 tgtcgcagta ataaacactt ctgacccatc gtaatctctt ttgataatcc ctattaccgg
     5461 taaatcaacg attcgcttga tttcattgat atcttctgtc gtatttgctc gaataccaac
     5521 tgctccgcct tcttttgcag ccaaagccat tctccccata ataaaagaac tgtgtagcgg
     5581 ctcgttttcc aaagcttgac aagaaacgac gactccgcct ttgatttgtt ccagtgtatc
     5641 cacaatatct cctccagtaa tgttatataa aaatacagct tcatataaaa atgtaaccct
     5701 tttctattca ggagacaata atgagctatt tctttcagca gacctttctt actaaaagaa
     5761 aattacttca aattcttcgt atacgaccag cttctcttct gtaaattcta tgttcaacgc
     5821 ttctttataa gcatgtttaa aaaactcagc atggactttc cgccaataag ctaatgtccg
     5881 atccccttct ccttctttcc aagcatgctc tttcgtgact tcactaaaag gcaagatggt
     5941 cacatttata tttttgatga tacaaactgg ttcatttttt ccattcagga ccacgtctaa
     6001 ttgaccaact tttggtaaag gctcattctc ttgtttatac agatcgtaac cacttgatgt
     6061 accagtcttt atctttttta caactagttc gctcaactcg tctgccattt tttctgtatc
     6121 cccaaatgaa aagaaaccta atttttctga ttgataacta ggatattttt cttggaactt
     6181 tttgacatat tcttctactt gctgatacat aacatccacc cctttgcttt ttataatgaa
     6241 aaaaccatcc agatactagt ataaacaatt atctggatgg tgagcataat gaattattga
     6301 atattcaaga cacgcagact atcttcaacg atttgttttg gtgttaaatg ataacgttca
     6361 taaagctgcc ctacaggaac gctatccgtg aattctttct ctgcaccaaa atttaatacg
     6421 ttcattgaag aatcaccata ataacgactg atattttcgc caaatcctcc tgacaaacta
     6481 ccatcttcta atgtgatgac taattcgtgt gtttctttta atttatctaa tgtttctacg
     6541 tcattttctg tgatcgttcc tggattgatc aacgttgctt cgattccttt ttttctaaaa
     6601 gcttttactg tttcttttcc taattcaaga aaaccgccta aagctaaaat tgctactttt
     6661 cctccattat ttactgtttg ataggctgga atactataat catttgcgat agctgtacca
     6721 tgttctacac catgtgtagg aattcggatt gctacaggct gcgctgtttg ttcgaacgcc
     6781 caatctagca tagagatcat atcctctttt gttgctggag caagatattt gatgttcggg
     6841 atacttgata agtaaggaat gtcaaatgtt ccttggtgtg ttcggtcact agctgagatc
     6901 gttccgccac cgactaaaat aatcactgga ttctcgttga tcgctaaatc atgtgataat
     6961 tgatcatatg cccgttgcaa gaaagtactt gattggaaga cgactggacg tactccctga
     7021 gaagccaaag cagaagcgaa agtgatagag tgctgttcag cgatccccac gtcaaaataa
     7081 tgatctggat gtttttcttg gaatgttttc agtccaaagg cgcctggtat cgctgcattg
     7141 actgcggcaa taggagctgt acttgtttgc attttcttat ccaaataatc catgatgatc
     7201 atattaaatg tttctccaga agttacttgc tttgcctccc cggtggacag atcaaatggt
     7261 acatgccaat gatatttcat tttattatcg attgccggct gataaccgcg acctttttcc
     7321 gtatggacgt gaagtacaat cggatgatca atatctttta cttcttcaaa taaacggatc
     7381 accgtttcca gatcgtttcc attatccacg taacgataat caaatcccat tgctttaaat
     7441 aggttattgc tgcttttacc gttggtttga cgaagctcag ctaagttttt atataaactg
     7501 ccatgattct catcaataga catctcatta tcgtttacta atacgattag attggattgt
     7561 aaaacaccac cattgttcaa tccttcaaaa gctaagccac cacttaggga gccgtcaccg
     7621 ataaatgcta ccacatttcc tgattctttc ttcagatctc tcgctttagc taaacctgtt
     7681 gctaagctga tagatgtaga tgtgtgcccc actgtaaaat agtcatgttt actttcatgt
     7741 tggttggaat atccgctcac ttcacggtaa tgtccttttt caaaaccatt ttttcgacca
     7801 gttaaaagct tatgaccata agactgatgg gaaacatccc aaacgatttt atcctttggt
     7861 gaatcaaata cataatgaaa cgcaatcgtt aattctacta cacctagatt cggaccaacg
     7921 tgcccaccta catcgctgtc cttttgcaag attttttggc gcatctcttc tgccaactgt
     7981 ttcatttcct caacagataa ttttttgaca tctgcggggc tattgattgc atccaataca
     8041 tgtaatactg tactcataat acattctctc ctccgtacta tcttctttta atgaaaatag
     8101 atttgttaca ctttatctat tctactactt agagttgact ctaagtcaat agcattttaa
     8161 aaaaatttca gagaaagctt gttatataag tagatcttcg cacgaatcag gactcaaata
     8221 aaaaccttct acagacaatt aaaaagcttt gtagaaggtc tcctaattaa ggtctcatca
     8281 agaaatatgt ttttttaata ggtgaagttc ctgagcaata tgatacaaat ggatgtttgc
     8341 accttttaat gagattggat agatctttgt cttttcagtt tctaatccat tttgatagct
     8401 agcagcatcg cacaaaacca ctcttttttt cgcacataga cattcatcag gcaattcttc
     8461 taatgaacga aataaaatag gatttatttc agcgatgcct tcttccgcat acaaatgaaa
     8521 ttgggttcga taaaaaaatt gtttgactga actttctgaa acaacaaaaa tttctatttc
     8581 ctgtctcccc gcttgttcac tgttgatcaa agaaagactt ttgacgaatt ttcgaatgaa
     8641 attttcattc catcttagtt ggatttgatt tttctttttc cactcttcca atatttctac
     8701 aatttttccg tacagaagtt gttctttttc tgatagaaga tagatccttt ccggtaaaaa
     8761 tacttgtcca tctgcccaca acgtacggac aaattgtaaa aaggattgct tgaaaataaa
     8821 attttctgca tcgtcgattt cagcgatact aataatctgt aaaatcaatt cattcagtga
     8881 taggtcattg agaacaaaat ttttataaac aacttcgaaa tcttttctta acagctccat
     8941 gttctcattg gtataatttc tactgttgaa caaagaaaaa atataataga catcttcttc
     9001 ttgtaagtct agaattcctt tttgctcttc caatccttga cagactaatt gaaataacgg
     9061 cttcgtttct gcgcttttct tatcttcttc tttcagtcca aggggaaatg cctttcttcc
     9121 gattgcgatc tctattcctc gtatgataaa ggacttttca tcttccgaat atcttctttt
     9181 aaaaaaatga gtctctacat aatcaatcaa ctcgttacaa gcttcttcta catacggatt
     9241 tttattttca tagccttccc aattgacata acgtactaaa gctagcaaga tgttccgcga
     9301 gtcaaattct gggataatga tttctttatt tttcaaataa tcattttctt taaaaaaatc
     9361 caatacgatt tttttgattg tatatacttt gctcaaagaa ataaattcaa gatcagccag
     9421 ttctgtactc gtgaatatac cattaaaata ataacttaaa aactttaaaa aataagattg
     9481 accgtacaga aactgtgtga gctcagaaag agcaatacca tgtttgaata cgagtttata
     9541 cttatgcttc tctcttactt gttcttttat acaatattct agacactcgg ctagactttc
     9601 tagatcgtga aggatcgttg gtgtagtcac ccttagagaa atagctgttt gttcgatgtc
     9661 tatttcttgg aattcaaata attgttcaca cagatacact tttctaaata tatttcgctc
     9721 gatgtaattt tccagcattc ctgatacacg cctccagttt ttcttatgat tttatttaat
     9781 catatctgta aatcgtattt ttgtttcctt tttatttaga aaagcccttt caaaaaccaa
     9841 taaaaaaaga aactgggaca aaagtcaaac aagctcacaa tttcagcctc attgtaggta
     9901 gctttgagcc gtttggactt ttaccccagc ctctttcaag aatggttatt caaagatgtc
     9961 agcgtctagt tctttgacta catctttcaa caattggata ccagctgcgc tgttaccaaa
    10021 tggatctagt gccgggctga agacacctat gccgtaacga tttggcacag ctgccatcaa
    10081 cccgccgcct acaccgcttt ttgcaggtac accaacatgg acggaaaact ctcctgattc
    10141 atcatacaaa ccagcagttg tcataattga tttgacgatt gtagcacttt cttcagtaat
    10201 gatttgtttg ccattccaag gagcaattcc tttatttgcc agcacagcag ctaattttgc
    10261 tatgcctgta gcggtgacat tgagggagca ttgtctgaaa taagcatcga gtatctccgg
    10321 tacgtctcct tcgatcattt gattaccttt catatagtaa gcaagagagc gattgatatc
    10381 acctgttctt gattcagaat gatagatttc ttcattcaat gtgatattag gatcatcaca
    10441 gatctctttc ataaatgaca agattcgctc aaatttttca tctgcatctt taccggcaat
    10501 caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga ttcattggat gtttgcgatg
    10561 attgatttcc atattcatga tagaattgaa ggcaaaaccg gttggttccg tgttgattgt
    10621 ttgaaagact ttttctattc cgttgtcttt gattgctaat aacaatacgg gaacttttga
    10681 catgctttcg atggcaaagc gcacatcggc gtctcctccc tcaatcagtt gttttgtatc
    10741 caaatcataa atcgccatac ctaattgttt gggatccact tcagctaatg ctggaatgta
    10801 atccgccact tgtcctgttt gataatagtg gaaatttttt ccaatcaatt gttctaattc
    10861 ttttacttcc atttgaataa ccagctttca ttttttattt ttctgcatgc tggaatttat
    10921 gacggcatgc gtagatcgta aatggaataa ctaagacgat aagataagaa acaatcagaa
    10981 tcgtttcata aatcccatgc tgtccagctg cgatactagc aggtggaaca aatgagatac
    11041 aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac gattactttt acaacttttc
    11101 cgcctggaac ctgataggct ctttcaagac tgtcatcttt aaaaaccagc ttcaaatagc
    11161 ccgcgaaaaa cataaagtac aacatcaggt agattacaac agtcaatgac atcgctgcaa
    11221 gaaatgaaag attattcccc ccgccgctta atgtaaagac ggctgcccag attgttgcaa
    11281 cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg tgtgttttct tttgcaaaat
    11341 attcaggaag taatccttct tctgctgcta cgtgaagtgc ttttgaagga gaggttaccc
    11401 aagaagcaat ttctccgatg acacctaaac aaacaagaat cgcaagaatt ttcgcaatcc
    11461 atgcaagatt tggatcaatg tgtcttaata ggaattctaa cgattgaatc acccctgtat
    11521 taagagacaa accatgttga ggtactgtag tagcgacggt caatccgcca aaagtatcta
    11581 aaatgattgc cagaataacc aatacaaaca ttgctaatgg ataatccttt ttagggtttt
    11641 ccatttcatt tgcatgactg gcagatgttt ctacgcccat atatgctaaa ataaaggaaa
    11701 caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat caatgttttc atagacagta
    11761 tcgtttgtag tggagcgcct gaagcgacat aatggatccc taagccaaac aagactaatg
    11821 ctggcaacaa gataccagct acaaaaccga tccgagcaat caatgaagtg tatttggttc
    11881 cttttaactg tgagagtaaa atagcccaga aaataatcaa taggattgcc aatttcagat
    11941 atttattgtt ttgtatagca ccaatacccg ttgcatagga taatgcacca gttaagaaat
    12001 acagcatggt caagtagcca actgttatct caaaccattg gaaaaagatt gcggcaaaac
    12061 cgaatcgttt tcccaatgtg cgactgaccc aagtatagac accgcctttt tcccatcctt
    12121 tcaccgttgc catctctgct gcgcacaatg ccacagggat aaaccatagc aaaccgccta
    12181 ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa tgtgggatat tcatataatg
    12241 aaacgaccat tgagatggtc atagccaaaa agccaaagaa cgttaatttt tttgcaggaa
    12301 ttttcgtttt gctttgttgc atgaatcttc ctcttttcta tttgaatttg tttcaattga
    12361 gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg ttcttctttt gtagtagtga
    12421 tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt cagcacatag gcatacacgg
    12481 cagtaatgat gacaccaatc acttgaatcc ctaagaagta ccagctgctt ggattcgtta
    12541 gtaaatggct gttagatgca aagatcccta tcagaatcgt acctgtgaat ccgcccatgc
    12601 catgaacacc ccacacatca agtgcatcat cccattgttt ctttttacga aaatcaacag
    12661 cagcatgaca gacgataccc gcaatgatcc caatcagcat ggcacttttt gcttctacat
    12721 aaccggcaca aggcgtaatg gctgccaatc cagcaacgga tcctgttagt acgtcgacaa
    12781 aatcaaagcc atttcctttt acttttgcaa tgatcagcca agtaatcatc gcaaatgcaa
    12841 gagcgatgaa tgttgaacca aatgcattga ctgcttgttc atctgctcga agtgcaccac
    12901 cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt accgatagca gctgccataa
    12961 gactgctatg ctgacttctt tctcctaaag gaatctttct ttgaccaagt gtgaggatac
    13021 atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc tccaccagcg aaatctctaa
    13081 atcccatctg atccaaaaat ccgccacccc atatccagtg gcaaacaggg aaataaatca
    13141 gcagattcca aaaaataaca agtataatat agccttttaa attcaatctc cctgcaaaag
    13201 ctcctgtcat caaaggaacg gtaatcacga taaacatcag ttggtaaaga aagaacatta
    13261 aaaatggaat cgttgcacca tgaaacatgt tggggaagct tgtaacatgt ctcatcaaaa
    13321 agaaatctgc cgggtttcca ataacaccgc cgatatcccg tccaaaggcc aatccgaatc
    13381 caccaaatac ccacataatc gttgttatcc caacggagag aaaagattga gacatgatcg
    13441 ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa agctagacca ggagtcatca
    13501 ggcaaaccat tgctgtacat aaaatcatga atgctgtatc tcctgtattg ataggtaatg
    13561 tattcatttt tttcgcctcc tttctacgat tacgagtata aaagaaggtg tagttgtttt
    13621 tttcttctgt ttttttgaat taatacttgc atttataaaa gacagacaat taataaaagc
    13681 ttgatgtgtt gtgctctt
//
LOCUS       DAYYAR010000076        13618 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000076 DAYYAR010000000
VERSION     DAYYAR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.076"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..1448
                     /locus_tag="ACHAT0_001842"
     CDS             21..1448
                     /locus_tag="ACHAT0_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451774.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="ACHAT0_001843"
     CDS             complement(1540..1905)
                     /locus_tag="ACHAT0_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8451775.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="ACHAT0_001844"
     CDS             complement(1902..2267)
                     /locus_tag="ACHAT0_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8451776.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="ACHAT0_001845"
     CDS             complement(2459..2707)
                     /locus_tag="ACHAT0_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451777.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="ACHAT0_001846"
     CDS             2706..2834
                     /locus_tag="ACHAT0_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8451778.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="ACHAT0_001847"
     CDS             2891..4585
                     /locus_tag="ACHAT0_001847"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8451779.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4685..4981
                     /locus_tag="ACHAT0_001848"
     CDS             4685..4981
                     /locus_tag="ACHAT0_001848"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451780.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5359..6492
                     /locus_tag="ACHAT0_001849"
     CDS             5359..6492
                     /locus_tag="ACHAT0_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8451781.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6523..6843
                     /locus_tag="ACHAT0_001850"
     CDS             6523..6843
                     /locus_tag="ACHAT0_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8451782.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6872..7336)
                     /locus_tag="ACHAT0_001851"
     CDS             complement(6872..7336)
                     /locus_tag="ACHAT0_001851"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8451783.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7467..8720)
                     /locus_tag="ACHAT0_001852"
     CDS             complement(7467..8720)
                     /locus_tag="ACHAT0_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8451784.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8749..9066)
                     /locus_tag="ACHAT0_001853"
     CDS             complement(8749..9066)
                     /locus_tag="ACHAT0_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8451785.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9092..9412)
                     /locus_tag="ACHAT0_001854"
     CDS             complement(9092..9412)
                     /locus_tag="ACHAT0_001854"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8451786.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9876..11291)
                     /locus_tag="ACHAT0_001855"
     CDS             complement(9876..11291)
                     /locus_tag="ACHAT0_001855"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8451787.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11308..12624)
                     /locus_tag="ACHAT0_001856"
     CDS             complement(11308..12624)
                     /locus_tag="ACHAT0_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8451788.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12774..13472)
                     /locus_tag="ACHAT0_001857"
     CDS             complement(12774..13472)
                     /locus_tag="ACHAT0_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8451789.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4315 a         2772 c         2349 g         4182 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
     4741 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
     4801 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
     4861 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
     4921 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
     4981 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
     5041 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
     5101 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
     5161 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
     5221 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
     5281 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
     5341 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
     5401 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
     5461 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
     5521 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
     5581 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
     5641 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
     5701 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
     5761 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
     5821 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
     5881 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
     5941 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
     6001 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
     6061 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
     6121 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
     6181 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
     6241 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
     6301 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
     6361 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
     6421 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
     6481 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
     6541 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
     6601 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
     6661 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
     6721 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
     6781 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
     6841 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
     6901 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
     6961 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
     7021 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
     7081 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
     7141 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
     7201 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
     7261 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
     7321 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
     7381 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
     7441 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
     7501 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
     7561 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
     7621 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
     7681 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
     7741 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
     7801 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
     7861 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
     7921 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
     7981 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
     8041 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
     8101 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
     8161 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
     8221 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
     8281 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
     8341 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
     8401 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc
     8461 aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat
     8521 tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc
     8581 attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc
     8641 gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata
     8701 aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa
     8761 tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag atgtcatcag
     8821 atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg
     8881 tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat
     8941 tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc
     9001 gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc
     9061 ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt
     9121 cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa
     9181 tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta
     9241 aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag
     9301 caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga
     9361 gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca
     9421 cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct
     9481 ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag
     9541 aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa
     9601 aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt
     9661 gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta
     9721 ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca
     9781 gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc
     9841 agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa
     9901 tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg
     9961 cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt
    10021 ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct
    10081 gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc
    10141 acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt
    10201 atcagccaag ggatattatg ataattttct tttagataca tggctatatc atacatgatt
    10261 ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact
    10321 tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga
    10381 acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt
    10441 tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac
    10501 tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca
    10561 tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga
    10621 tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta
    10681 gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc
    10741 ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta
    10801 aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa
    10861 aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact
    10921 tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt
    10981 tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa
    11041 cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca
    11101 tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa
    11161 acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct
    11221 ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata
    11281 gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac
    11341 ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac
    11401 tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg
    11461 gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc
    11521 agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat
    11581 accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat
    11641 ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac
    11701 tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac
    11761 ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc
    11821 ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat
    11881 aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac
    11941 gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc
    12001 agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac
    12061 attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca
    12121 ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt
    12181 atattgttta gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata
    12241 aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc
    12301 cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa
    12361 acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa
    12421 gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc
    12481 aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat
    12541 ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat
    12601 aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc
    12661 tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat
    12721 ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta
    12781 gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc
    12841 attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga
    12901 atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt
    12961 gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc
    13021 atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt
    13081 tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct
    13141 gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc
    13201 cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca
    13261 ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc
    13321 acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc
    13381 ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta
    13441 gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta
    13501 ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg
    13561 attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa gactagga
//
LOCUS       DAYYAR010000077        13587 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000077 DAYYAR010000000
VERSION     DAYYAR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.077"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2356
                     /locus_tag="ACHAT0_001858"
     CDS             158..2356
                     /locus_tag="ACHAT0_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451790.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(2938..3810)
                     /locus_tag="ACHAT0_001859"
     CDS             complement(2938..3810)
                     /locus_tag="ACHAT0_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8451791.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            4110..5084
                     /locus_tag="ACHAT0_001860"
     CDS             4110..5084
                     /locus_tag="ACHAT0_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8451792.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            5106..5798
                     /locus_tag="ACHAT0_001861"
     CDS             5106..5798
                     /locus_tag="ACHAT0_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8451793.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            5764..7272
                     /locus_tag="ACHAT0_001862"
     CDS             5764..7272
                     /locus_tag="ACHAT0_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8451794.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            7346..8449
                     /locus_tag="ACHAT0_001863"
     CDS             7346..8449
                     /locus_tag="ACHAT0_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8451795.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            8452..9789
                     /locus_tag="ACHAT0_001864"
     CDS             8452..9789
                     /locus_tag="ACHAT0_001864"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8451796.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            9776..10654
                     /locus_tag="ACHAT0_001865"
     CDS             9776..10654
                     /locus_tag="ACHAT0_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8451797.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            10651..11574
                     /locus_tag="ACHAT0_001866"
     CDS             10651..11574
                     /locus_tag="ACHAT0_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8451798.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            11571..11981
                     /locus_tag="ACHAT0_001867"
     CDS             11571..11981
                     /locus_tag="ACHAT0_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8451799.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            11985..13553
                     /locus_tag="ACHAT0_001868"
     CDS             11985..13553
                     /locus_tag="ACHAT0_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8451800.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT         4545 a         2243 c         2868 g         3931 t
ORIGIN      
        1 aaaaaaatat gcggcgcaca gaacgcgccg catatcggac taagttttat tcaaatttgt
       61 cttcgccaac acaatttgac gaagagcctt aattagatga gtatcaattt tacagaaaaa
      121 tgaaagcctt ttattttaaa aggaggagag gaagaaaatg aagaaaagta cagtatttta
      181 taaaatattt ttaccgatgt ttttattagg agccgtactt gtcataggat ttagtttatt
      241 catttaccag aatacctatg aatcgatcga atcgagttat ttaacagaca aaaagaattt
      301 gctcaggcaa gttaaaacta acgttgaatg gaaaattaga acgattgaat attcgttttc
      361 tacgtatggt tcgaccaaaa atttctcgga catattcaag catccgctga catatacaga
      421 ttactccact tattctgaaa tcaggaaaga actaaatttt atagaaacga ttgttatgga
      481 tgataataat tatgatttag tcagtttggc tggtaaatgg ggagtaatca atggatcgtt
      541 gagtcaactg accgaagagg aagttgaaaa gtatcaacag aagtacataa ataacaaaaa
      601 taatttattt tggcaaaaga aaaacaaagg aatcgaaatg gtgatttctt tgccaatgtt
      661 tgaaaaagaa aaatatgcat tgggaattgc tacgatagag aaacatacaa tagaacaagt
      721 tgttggtgaa agtggagaag acatactttc tattgaaaac gaagttggca acctttttac
      781 tactcagaca gtaaaaaata aagagcaacc aactgtatta acggacgtgg atctagatta
      841 caatcagctg aaagtgataa gagaaaaaga taaaacgtat atagtactga aatcagatta
      901 taataattgg atttacagat tagaagttga ctctagtgca attggcgcaa tgatccgcaa
      961 tctgagaatc gggctagttg cagtgtcttt aacactggta ggactcattg gtcttttttc
     1021 ctacttattt tcggacagat ttgcccgtcc gatttctcaa atacaggaac gtctgaatct
     1081 taaatcaaaa ggattcagtg ggaaagaact agatttggtc gctcaatcag tcagtcggat
     1141 cataggagaa aaagaagcgt taagtgcaca cttagttacc caaaagccac agctggaaac
     1201 gctatttgta cttagtttat ttagaaatcg agtagaaagg cgagaactag atcagcggtt
     1261 gcagcaattt ggttatagtt ctcaaaatga ctgctattat acagctttgg ttcaaatcga
     1321 tctgttagaa gatagtcacg gtggggagcg tgatttgcta cttttagcga tcaacaacat
     1381 gatcacggaa attgtgccaa aggaaaatcg aatgctgccg atcgttttga atgaagaaat
     1441 gcaagccact atttatcgaa tggataaaga tgatgcagaa gcaagcaaaa aagtcatgga
     1501 gtattgccga ctcattcaaa aacgtgtaaa agaatacttg aatttaacta tcagtatagg
     1561 catcagtaac cggttcgaaa cgctgaatga aagcaagcaa tcggttgatc gggcaaaaga
     1621 agccctgtat tatcgtgtaa atactggtcc aagttcaatt attttttatc aggaaatcgt
     1681 tcctgctgct catgaaaaaa cacttattag atatccgata gaagaacaaa atcgattgtt
     1741 tgaagccatt cgttctggaa atcaggaagt gaagaattta gtacatgaat tgattgatgc
     1801 attgtttgca caaaataaaa atcctttgag tcgggaagta gtttcggtcc gcttgatcaa
     1861 tgaactcgtt caattaggac agttattggg agtagattca aaaaactttg ataacatgaa
     1921 acaaatttat gtaaaagcac tgaacaatta tcatcccaaa gaactagaaa aacttgtttt
     1981 agaacaatta gttctgccaa tcacagaatg ctctcaagca atcactgatc atgaatttaa
     2041 aaatttgtcg gagaatatta tgcatatcgt ctacacagaa tatgatcgtg atctgtcttt
     2101 agatgtgatc gctgataggt tgcattataa ccctaactat ttaagtaatg tatttaaaaa
     2161 ggaaacagga gaaaaatttg gtgattttgt tcaaaatcat cgtttagagg tagcaaaaaa
     2221 atggttgaaa gagactaacc tctccgtaaa agagattgca gaaagattgc aatatcgaaa
     2281 tccgcaaaat tttattcgtt tctttaaaaa gaaggaatcg attacgccgg gagaatatcg
     2341 aaaaaaatat ttgtgaatca agaggctgtt tttcttgtga tctgattcaa gcaataaaag
     2401 aggacagcaa aaaacagcac ttcatgtttt gctgctggct gattttattg ccgcagaatc
     2461 aacaagataa acgccgttta atcaagaggc tgtttttctt gtggactgat tcaagcaata
     2521 aaagaagtta gaataaccct agtatttgta tttatggata gttaaatttc aaggctaagc
     2581 acgcaaaaag aactgtgaca aaagtcttgt cacagttctt ttttacgaat aaatggcgtt
     2641 caagagacag gctatcgaca ataaggcata attttgtgaa atatgggaag ctatttttca
     2701 aaatcgcttc ttattgctcc tgtgggttac tcgtcgcaat gtttgtgaga gtttttatca
     2761 tcgctcaagg ctgaacgtct ctttcacaac ctctgataca cggtgttcca ggagtagatt
     2821 cttcgaaaaa taagccgaaa tttcacaaaa atttaagaag caattttcgt aaatgccttc
     2881 ttatttcttg aagctgacca cttctgacac aactaccatt ttgcgttcag tacataatca
     2941 ccctttgaca gaacctacga gtgctccttt gacaaaatat ttctgaacaa aaggataaac
     3001 aatcagcata ggcactgttg caataacgat aacagccatt ttaactgttt gtgctggtgg
     3061 aatcacatca acggcactac tttctgcttg catcccactt gatacaatga cgatttggcg
     3121 taaaagaatc tgtatcggcc atttagatga atcgttgata tacaaaatag cagtcatata
     3181 agtgttccag taagtaacag cataaaaaag cgagatagta gcaatagacg gtaaagctaa
     3241 tggcagcata attttgaaaa atacaccaaa atctgtgcaa ccatccattt ttgctgattc
     3301 ttccaaacta tccggcaagg cttggaaaaa gtttctcata atgatcatat tcattgcatt
     3361 aacagccaca ggcaggatca tggcccagta agaattgacc agccctagag aacgaacaac
     3421 taagaacgtt ggaatcatcc ccccgctgaa caacatagaa aatacaacaa gaaagttgac
     3481 gaaacctcga ccaaacaaat actttctgga aagaccgtag gccatcagtg aagttgcaca
     3541 catactgacg atcgttccga cacctgtaac accaatcgaa acagcaagtg ctctaaaaat
     3601 ggtcggcgta gaaagaatgt agcgatacgc gtctaaagaa aaggtagtag gaaaaaggat
     3661 aaactttttc gcgacgactt cttgtgtaga agcaaaagat gtagcgatga tatttaaaaa
     3721 aggaacgata cagatcaaag ctagcaaaat tagaaacgta ttgttgaaaa gtgtaaaact
     3781 tctttctcct atagagactt tctttttcat atagataggc tcctcctcaa aataaattaa
     3841 atgtttctgt ttctatatgt tcaatttatc atcggtgaaa gattacgtat ataatcattt
     3901 cctatgctaa gtgtcagata gaaaaaaata gaaggtttgc tatgcgtagt ttatttgtga
     3961 aaaaatacac tttatcgttt tgtatagcac aaaaatcatt gctatgtaag cgtttttact
     4021 tacggtaaaa atgattatat acgtaattca gatagttatc tatcatgaag tcataagcag
     4081 caaacattca caaaactaag gaggggctta tggaaagtgt aaagaaacaa gaaccatttc
     4141 aactgacgct atcgaaaaaa gaagaaaagc tcagaagacg aaaaaaaata attgcaggaa
     4201 tcaaaacgaa taaattttta tatgtgatga ttctgccggg agtcctttat ttcttgattt
     4261 ttaggtatct tcctatgggg ggactcgtta ttgcttttca agattatcaa ccatttttag
     4321 gaataatggg aagtccttgg gtcggtttca aacatttcat tcgtttgttt acagaaccga
     4381 cattctttac attattaaga aatacgttga tcttgttcgg attaaatatc ttcgtgtttt
     4441 ttccgatgcc aatcattttg tctttactat taaatgaagt aacgaacaaa cgattgaaaa
     4501 acgggattca aacgatcatt tacatccctc acttcatgtc ttgggtaatc attgtttcga
     4561 ttacttatgt atttttgaat gttgacggag gggtattgaa tgaattcctt gcaacaatgg
     4621 gatttgaaaa aatcagtttt ctaacatccc ctgaatggac acgcacagta tatatcggac
     4681 aagtgatctg gaaagagttg ggctggtcta cgatcatcta cctttcagcg ataactgcag
     4741 tcgatacaca gttgtatgaa gctgcagaaa tggatggagc aggacgctta cgcaaaacat
     4801 ggcatgttac attgccggca attcgtccag taatcattac cttgctgatt ctaaaaatcg
     4861 gtcagacatt agacttaggg ttcgagcata tgtacttgtt attgaattca ttgaatcgaa
     4921 gcgttgcaga aattttcgac acctatattt atacagctgg attgaagaat ggacagttaa
     4981 gtttcagtac agcaatggga ttgtttaaag gggtcgtagg attgtttatg gtaattttag
     5041 ctaataagat tgccaaaaag ctaggagaag acggcgttta ctaagataga aagagaggga
     5101 aagatatgct gcagtcatta gataaaataa acggaaggct aaatcgtatt ttacagttgg
     5161 tttatttgaa ttttctgtgg ggcggtttag tcatcgcagg tttgggagtt tttactttcg
     5221 gtcctgcatc gtatgcgatg gttgcagtga ttagaaaatg gctgcgctca aaagaaacgt
     5281 ttcctttgac gaaaacgtac ttcacatatt ttaaagaaaa ctatcgtgaa accttgctga
     5341 tgagctggat atatggcgcc atcgggattg ttttacttgt ggatctttct ttcatacaca
     5401 attggtatct taggatcgct ttgtatattg ctgcgttcat atatgtgctc tcagcactct
     5461 atattttccc aatcatggct cattatgatt ggaaaggata ttttttcaaa atgaaaatgg
     5521 catttttatt cggattctca catcttcatt actcattagt gttgatatta agtatggtag
     5581 ggatttatct gatgatcatc caaacaatgc caggtgtttt aacctttatc ggtggcagtc
     5641 tcttcttttc cgcagtcacg tggacagcaa attctatatt ttgccatttg gaatggaaga
     5701 actcatatac agaaaaaaca gaagaaaaca aaaatattat taaaacagtg aaggagaaaa
     5761 tttatgaaaa aagcaacgct cgtaaaatta ggtcttagtt ttgcaggaat tctagcactt
     5821 gccgcatgcg gcagcagttc aagtaacagt tcttcagaga cagaagatgg aaaagctaaa
     5881 atcacttggt taaatatttt gcatcatgct tcaccgccta cagacacgat tgtgaacttg
     5941 atcgaagaaa aaaccgatac ggaactgaca ttcaactgga ttcctgatgc ttcaaaagaa
     6001 gaaagattaa caactgcatt ggcttctgat gaactaggag atatcgttac acttcaaatt
     6061 gcgatgttga aaaactcttc tgtccgcaat gcgttaaaat caggcgtatt ctgggatgta
     6121 tcagaatatt taaaagacta taaaaattta agtcaaattt ctgaagatag aatcgaagct
     6181 gcgagtatcg acggtgcctt gtacggcgta ccgatacaaa aagactacgc tcgtgctggc
     6241 ttagtcattc gaaaagactg gttggataat ctaggactag atgttccaac taccatggat
     6301 gagctttatg aagtagctaa acaatttaca gaaaacgatc ctgatggaaa tggtgtggac
     6361 gacacagttg gttttggcga tcgtgcgcct agtgatattc gttataccag ctttaaactt
     6421 ttcacttctt attttggagc accaaatggc tgggctgtag acgataatgg caaattcact
     6481 ccagaatttg atacagaaga gtatatgaaa gctttgaatt tttcaagaga tctatttgag
     6541 aatggctatt tggcacaaga ctttgcagtt acccaaaaga ctgatcagca agaacaattt
     6601 gcacaaggaa gaactggaat ctacactgga atggtggata tcaagaatct gaagacgatg
     6661 ggtcaaggta ttcaagatga tatggaatta gtgcctgtaa acaagatctc agatggtgaa
     6721 ggcggcggtt atcatgtttg gtcagaagga aatggtattg gcggcttgct agcatttccc
     6781 aaatctcaag taaaagacga agcacaattg aagaaattac ttcaatttgt tgatgatctt
     6841 ttagaagaag atgtgtataa attgatgact ggcggtatcg aaggaacgca ttatgaattg
     6901 aatgatgatg gttctatcga atttatcgat atggatcttt ggcaacaaga ggtgcaaccg
     6961 ttatcaagct ctcgtcctag tgaagtgacg ttctctttca aagatacaga ccctgaaaaa
     7021 gaattctcta atcaattgat caaagaaaat gaagaatttg ctgtaatgga tccttctgtg
     7081 ccattggatt caccgacgaa caatgaagta ggatcagaaa ttgaaaaaat cgtcattgat
     7141 gctacggtgc aatacatcat gggacagctt gacgaagatg gattcaaaaa agctgtagaa
     7201 gactggaaag cacaaggagg cgatcgaatc acacaagagt acgaggaagc atacaaagca
     7261 gcggcacagt aatatagacg attgggacaa atacttcttg gtatttgtcc caaatcctat
     7321 tttaattttt aaaggagagc aaataatgga aaactggtca aagaaattag caaaaagtat
     7381 catggaaaga acacctcgct tatatgaaga aaagtggtat aaaggaaaat ggtcttatga
     7441 ctatggcgtc gtacttaaag ggttccaatt actttgggaa cgaacccaag aaaaaattta
     7501 ttttgatttc ataaaagaca atatcgacta tttcgtgcaa gaagatggaa cgatccgtgg
     7561 ctacagcgtt gaagaataca atatcgacca tgtaaataca ggaaagcttt tctttttatt
     7621 gtacaaagaa acaggcgaag aaaaatataa aaaagcagca gaactccttt cccagcagtt
     7681 ggcaaaccat ccacgcacat ccgaaggagc tttttggcat aaagaaattt atccttatca
     7741 aatttggttg gatggcttat atatgggttc tcctttttat gctgaattta tccagaattt
     7801 tagtgaagaa aaagattacc aagatgtttt gagacagttt gaaatcagtt ataaacatct
     7861 attcgatcca aaaacaaatt tgctgattca tgcatgggat gaaaagaagg tccaaccatg
     7921 ggctgatcca aaaacaggtc tatcccatca tttttggagc cgatcgattg gctggtattt
     7981 gatggctgca gcagatacct atgaattact tcatgaagaa acagattgtt ctttgctgaa
     8041 agaaattcta gaaaagacac tggctgcatt acttccatat caagaagaaa acagcgggac
     8101 atggtatcaa gtaacaacag aaacaaaacg gaaaggaaat tatctggaag cttccggcag
     8161 tagtatgttt gtttacgcaa tggctaaagg aatacgtttg ggactgctga acaaagaaga
     8221 atggcttcca caagtgaaga aatcacatca aggattactt gatgaatttg ttctggaaac
     8281 aaaagaaggc tggctgaact tgaataaaaa ctgtcaagta gcagggttag gcggagcaga
     8341 tcagcgagat ggttcatatg cttactacat cagtgaacca attatttgta atgatcaaaa
     8401 aggcgttggt gcattcttgc aagcattgat tgaagtggag gcattataaa tatgtatgat
     8461 attttgacat ttggcgcatc aattgatgaa ttgaatactg aagcaataca acaagccatt
     8521 gatgctgcag caagtgacgg aggcgggacc gtagttgttc cagcaggtga atttctaaca
     8581 ggagctttat ttttaaaaag caatgtcgaa cttcatttaa gcgcaggagc ggttctgaaa
     8641 ttctctgacg atccaaaaga ttatcctgtt gttcattcac gttgggaagg agtccatcga
     8701 aaagtttatg cttcctgtat ctatgctcaa aatgtagaga acatctctgt cactggtttc
     8761 ggtacattag atggaaatgg aaaaaaatgg tggcatactt ttagaaatga accagataat
     8821 ctcgcttatc ctcgtcctaa attgatgagt ttccataact gtcatcgaat tactgtgaaa
     8881 gatattaaat tgatccagtc accaagttgg acgatcaacc cgattctttg cagtaatgca
     8941 acgtttgata acttgacgat cttgaaccca gcggattcac caaatacaga cggaattgat
     9001 ccagaatcat gcaaaaatgt gcgtattagt aattgccata ttgacgtggg agatgactgt
     9061 attgcaatca aggcagggac tgaagatacg tacgaaagga tcgcttgcga aaatatcacg
     9121 atcacaaatt gcacgatggt ccatggacat ggcggtgtcg tattaggaag tgaaatgagt
     9181 ggtagtattc gtaatattac catttcaaat tgtatcttcc aagaaactga tcgaggaata
     9241 cgattgaaat ctagacgagg tcgaggcggg atcgttgaag atattcgagt atccaatata
     9301 gtcatggata atgtgatgtg tccatttata ctgaatcttt attatttctg tggtccgcga
     9361 gggaaagaac cgtatgtatg ggaaaaaaag gcttatccga tagatgaacg gacaccagca
     9421 ttcagaagga tccatttttc gaatatcacg gcaaggaatg tccatgcttc tgcaggtttt
     9481 atttatggat tagcggaaca attcatacaa gaaatcacat ttgacaatat cgatgtctcg
     9541 atggcccaag atgcagttcc cggtgttcct gctatgatga caggaataga ggaaatgagc
     9601 agacaaggat tttatattgg ctttgcgggt aaggtttcct ttaaccagat gtcgattgaa
     9661 aatcacgagg gaccagcatt tctattagaa aatactaaag aaatcagtat tgagaaattc
     9721 caatcaaatc aatcagaaaa tatggatgaa ctggttgttc gtaaaaactt tgcttatgca
     9781 gttgaatgat tttttaggtg ctccatctag cagacatacc cctagtggaa aacgattata
     9841 ccgagaaaaa caaaacggtt ttatgcttga atactatcaa ttgtacttaa atgaattgga
     9901 aacagtacca gccctctatg cttatccaga aaaagaagga ccgtttccgc cggtccttta
     9961 cctgcattct cacggcggag atttttcagt cgggaaaagt gaattgatcc atggtgctcc
    10021 ttacttagct tctccatctt ttgcagaagt actgacagga atgggatatg ctgtttggtc
    10081 gattgatgct tggggatttg aagaacgcgg aggtatttct gaaagcgagt tgttcaaaca
    10141 atttttatta acaggaaaaa cgttatgggg aatgcgtatt tatgatgtga tcagcttgat
    10201 cgattatttg gagacaaggg aagacacaga tactcaacgg ctcgcaacga ttggtctttc
    10261 tatgggaggc ttattaagct ggtgggtgac agcattggat tctcgtgtca aagtttgtat
    10321 tgatctagcg gcacaggtgg acatcgaaat acttttgcta catcgccgcc tagatcatca
    10381 tgggttttat tactatgtgc caaatctatt gacagcttat acaacgttag caatccaaga
    10441 aaaaatcgct ccgcgccctc gtcttagctt agttggaaaa aacgatacga tgtgcttgga
    10501 cgaaggcgta ctgaagctga gaaaagggct ggacaaaaag tatcaatcaa tgggtagtcc
    10561 agaaaacttt tcttcttttt ctttaacagg cggtcatatg gaaacacaag aaatgagaaa
    10621 tatttggaaa caattcataa gggagcattt atgaggatga aagacaaact gataattgga
    10681 aagaatgcta tagatacccc gacggatttt gaaactatcc aagaaggaat cgattatttg
    10741 gcaggcctgc cggatagcca tccaaaggtc ttgatagtaa aagaaggaag atatgatgaa
    10801 tatatagaaa gcagattatc caattttaaa cttgtgggag caggaacagt agttatctcc
    10861 ggtaatcgtt ttgcaaaaca ggagtatctt gacggtagcc agttagggac tttccgaagt
    10921 gctacttttt tcttagaagg atcgaatgtc gtattagaaa atcttcacat cgaaaataca
    10981 gcaggtccag gagaaaaggt aggacaggct gttgctttat tcaattcagg agatcgcgta
    11041 tctttaaaaa actgtcgact atctggttat caggatacct tatgcacggg tccgttgcct
    11101 gatttgcaaa aagatggtac tcctttttgt acaccggctt ctgccaatcc tgagtattgt
    11161 cgtcagacct atgaatattg ctggattgaa ggaacggtgg atttcatatt tggtggggca
    11221 gatgccgttt ttcatcagtg tcatatccat agcttagcca gtaaaaatcc tggatacatc
    11281 acagcagcat ctacgccgaa aaatcaaaaa acagggtatc gttttgaaag ctgtaccttg
    11341 actgcagaac ctgatactag aaacgtctat ttaggaagac cgtggcgtcc gtatgcaaat
    11401 gtcttatttg aagcgtgtaa gataggtggg catgttcacc caactggttg ggataattgg
    11461 gatgacaaga aaaatgaaca gactgtcaaa ttctctgaac gtaataacaa ttatgaaaca
    11521 tcgatcaaaa gacctgaatg gatacattta gaaaagcagg gggttcagaa atgaaaagaa
    11581 aatgggaact attgctagga atggtcggtg gcagtctatc tttgattttc tttggcgggt
    11641 tagcagttac tttaagtaat atgtctgcgt cagagtttaa aaaaagttat cagtcgcttg
    11701 ctgtagatca ttcaactttg tctttagaaa atacatttgg attattacag gacatgactg
    11761 gtctgtttgc cgttgtgttg tttatttcac ttgctttttt agcagtagca ttgtttttga
    11821 ctgctaaagg aaaatactta acaactgcca ctggtttgta ttttataaca ggattcatat
    11881 tgttgattgg cactcagttt attgcgtttc cttttgcatt tttttatttc gcagctgggg
    11941 cattcagttt atatcgagta agaatgagaa aaggagcctg aatcatgcag actatcaaag
    12001 tgaaaaatcc tattttaaga ggattcaatc cagatcctaa tatattaaga gtgaaagagc
    12061 ggtattacat tattgtctcc tcttttgaat ggcttccagg aatccgagta tatgagtcga
    12121 aaaatttagt caactggtca catacaacgg atattttgac cactcaagta tctttgcaag
    12181 gaaatcctac aaatggaagt atatgggcgc cacaaatcag ttatgctgat ggattgtttt
    12241 atcttgttta cacagatgtc aaaagtgcag gccgtccgtt taaagatgtc cacaattatt
    12301 tgatcactgc atctgagatc acaggtcctt ggtccgaacc aatttattta aatagtaacg
    12361 gctttgatcc ttctttgttc catgatcttg aaacgggaaa aaaatggctt gtcaatgaac
    12421 tatgggatta tcgtctggac acaccgaata aatccagcgg tatcgtatta caagaatatg
    12481 atgcagagaa gaagaagctt gatggtccca tctataaaat atttgcaggt acagaattag
    12541 cgaaaacgga ggcaccacat ttgtattttc gtaacgggta ttattatttg atgacggcag
    12601 aaggcggtac aggaagagga cattcagtta cagtttgccg agcaaagaat attactggtc
    12661 cttacgagct tgatcctggt tttcctatgc tgacagctag tgacaaacct gcttctacat
    12721 tgcaatgtac aggacatggg agcttagtac aaaccccgtc aggaagatgg tacatgacat
    12781 atctttgtac cagaccatta aacggtgcgg ctattttggg aagagaaacc gctattcaag
    12841 aagtgtattg gacgtccgac gattggttaa gacttaaaga tagccagaca actccgatgg
    12901 aagaaataat gattgagaca aatgaagagg tacaacaaga aaaagatcat cagtttatgg
    12961 atacatttga aggagagcta aagaagaact ggaatgccag acgcattttg ccaaatgctg
    13021 attggtgtga tacaaaatgt agaccaggtt ttttacgtct gatttccggt gaatctcttc
    13081 aatctacctt cgaacatcat ttgttagcta ttcggcaaac ggattttact tttgatgcgg
    13141 agacagcatt tgaatacatg cctaataatt ttaatcagat ggctggactg gtactgtact
    13201 taaatgaatc caattatatt tattgttatg ttacttggaa tgaggacaag ggaaaatgcc
    13261 tacgtatgat agaatcagaa aacggagtag cacaaataga agattggacg attccactca
    13321 cagaaaagaa gacttttttg aaagtcactg taaagaaaga gacagcacag tttcatttga
    13381 aagacgtatc aactgaagaa tggaagaaag taggtccgca aaaaaacatg tgtatactct
    13441 caggagggtt tactggaaat tttatcggga tagcagtgca tgatctaaat aaaaaaagag
    13501 gaagttatgc tgattttgag tttttcaagt atgatggaaa agatcatctg taatttaatt
    13561 ctttaataaa tgaggttcac taactta
//
LOCUS       DAYYAR010000078        13209 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000078 DAYYAR010000000
VERSION     DAYYAR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.078"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHAT0_001869"
     CDS             99..746
                     /locus_tag="ACHAT0_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8451801.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHAT0_001870"
     CDS             1139..3784
                     /locus_tag="ACHAT0_001870"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8451802.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHAT0_001871"
     CDS             4094..5407
                     /locus_tag="ACHAT0_001871"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8451803.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHAT0_001872"
     CDS             5397..6050
                     /locus_tag="ACHAT0_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8451804.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACHAT0_001873"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACHAT0_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8451805.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACHAT0_001874"
     CDS             6947..7147
                     /locus_tag="ACHAT0_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8451806.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACHAT0_001875"
     CDS             complement(7401..9080)
                     /locus_tag="ACHAT0_001875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8451807.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACHAT0_001876"
     CDS             complement(9082..9294)
                     /locus_tag="ACHAT0_001876"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8451808.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACHAT0_001877"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACHAT0_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8451809.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACHAT0_001878"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACHAT0_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8451810.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
BASE COUNT         4169 a         2307 c         2846 g         3887 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttatttt
//
LOCUS       DAYYAR010000079        13182 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000079 DAYYAR010000000
VERSION     DAYYAR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.079"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..289
                     /locus_tag="ACHAT0_001879"
     CDS             <1..289
                     /locus_tag="ACHAT0_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451811.1"
                     /translation="VEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTR
                     SAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(386..820)
                     /locus_tag="ACHAT0_001880"
     CDS             complement(386..820)
                     /locus_tag="ACHAT0_001880"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8451812.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1052..1762)
                     /locus_tag="ACHAT0_001881"
     CDS             complement(1052..1762)
                     /locus_tag="ACHAT0_001881"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8451813.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1752..2612)
                     /locus_tag="ACHAT0_001882"
     CDS             complement(1752..2612)
                     /locus_tag="ACHAT0_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8451814.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2617..3255)
                     /locus_tag="ACHAT0_001883"
     CDS             complement(2617..3255)
                     /locus_tag="ACHAT0_001883"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8451815.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3270..3569)
                     /locus_tag="ACHAT0_001884"
     CDS             complement(3270..3569)
                     /locus_tag="ACHAT0_001884"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8451816.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3612..5096)
                     /locus_tag="ACHAT0_001885"
     CDS             complement(3612..5096)
                     /locus_tag="ACHAT0_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8451817.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5116..5577)
                     /locus_tag="ACHAT0_001886"
     CDS             complement(5116..5577)
                     /locus_tag="ACHAT0_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8451818.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5595..6662)
                     /gene="ulaG"
                     /locus_tag="ACHAT0_001887"
     CDS             complement(5595..6662)
                     /gene="ulaG"
                     /locus_tag="ACHAT0_001887"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8451819.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6880..7650
                     /locus_tag="ACHAT0_001888"
     CDS             6880..7650
                     /locus_tag="ACHAT0_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8451820.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7923..8750)
                     /locus_tag="ACHAT0_001889"
     CDS             complement(7923..8750)
                     /locus_tag="ACHAT0_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451821.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9318..10553
                     /locus_tag="ACHAT0_001890"
     CDS             9318..10553
                     /locus_tag="ACHAT0_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8451822.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11552..11812)
                     /locus_tag="ACHAT0_001891"
     CDS             complement(11552..11812)
                     /locus_tag="ACHAT0_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8451823.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11839..13140)
                     /locus_tag="ACHAT0_001892"
     CDS             complement(11839..13140)
                     /locus_tag="ACHAT0_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8451824.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4174 a         2752 c         2075 g         4181 t
ORIGIN      
        1 tgttgaattt gacagtgaac tacttaagca atttccacaa gaagaacatt tcatgtataa
       61 agaagcaatt tcccacaaag tgacagagta tttggagcat aatttttttc gtataaagcc
      121 agtgcgtact cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa
      181 ttatcgcata gcatttgctt taaaaaaaaa tcatgtgttc attttttata ttagttcgaa
      241 tttgcaaaaa attgtatttg ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg
      301 aggatagatt gagtgatact aagtctatcc tcttgttttt ctaaagaact aagctagaat
      361 tttttctttt gtacgttatt atatgttatt tgtcattacc aaaaagcacc ttttttcttc
      421 tagtatctat gacataagaa ataccaccca tttcctttac ttgaacaaat cctactgatt
      481 cataaaattt ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg actggtccat
      541 aactagtgat cttctccaaa gcttcattca aaagcacttt gcctattcct tggcgctgat
      601 acctcggatg aatgaggata tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac
      661 gaacgattcc gataatcttc ccttccccat tacgctttat cagagattca gattgttcaa
      721 atgctctagc taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata
      781 attcagaaat ctcatcgatc ataagggaat caatattcat ttttttcttc ctttcttata
      841 caattctccc tctaataata cagttctttg actatttaat ctatcacata taactctcct
      901 gtaaagattc tctcgattcc ttaaaattcc tctttccttt tagaatgcat actttttgat
      961 aaataaaaag gttgtgataa attttcttca caacctctgt ttctctctat caagtgttat
     1021 cttttaattt tctaccctta ccctagcatc tctattgccc gtagtatgca tactttccat
     1081 gtttgcgaaa ataatgttta tctagtaaat attgcgggat ttcacatata ttggggttga
     1141 tcagctcgtt ttctttggcc atcagacaaa tttcatctaa gatcaaactg ttttctactg
     1201 ctttcatagg tgaatccccc catgtgaacg gtccatgtcc atagacaagt actccaggga
     1261 cttcgtttgg atctaatttt ctttccttaa acgtctcgac aatcacattc cctgtatgaa
     1321 cttcgtaagc ttctcttact tcttctttcg ttaattggcg tgtacaagga accttaccat
     1381 aaaaagcatc agcatgagtc gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca
     1441 tgacggagtg cgtggaatga gtatgagtaa tagcagttat atcttcaaaa gtttgataca
     1501 aaacaacatg tgtggggaga tctgaagatg gttttaagct atcttcttct attatatttc
     1561 ccgacaaatc tgttaccacc atttgatctg cttgcatgca ttcatatctg acaccgcttg
     1621 gcttaatgac gatgactcct agctcacggt ccacttcact cacattgccc caagtcaatt
     1681 tgactaatcc gtatcgcggc aatgccagat ttgcttcaag gacacgttgc ttcattgctg
     1741 caattttttc ttcacaagac atcatagcca gcctccttca atttagaact taagtagtct
     1801 tttgcctgct ggatttctct tcgaaagtct tggctttttt cactccacat ttcaatcaga
     1861 aatgttccat cataatctag acgtttcaaa tgtcttaata atccaagaaa atcaacacaa
     1921 ccgctaccaa aaggaacatc acgaaactgg cctgaatatt cttttgttac agggtaagta
     1981 tcttttaagt ggatagcagt gatataatcg atccctttct caagttctcg agccggatta
     2041 ttttccggcc atgcggacaa attgcctagg tctggatata cttgtagaaa aggtgaatga
     2101 atctgttctt tgatttctaa aaattttgaa atggagttca taaatggatc atccatgatt
     2161 tccacggaaa gaatgatgcc atatttagaa gcttccttta cgctttcttt tattccttcg
     2221 ataaagtctt ctctggaatt cattgatttc tcttcataat aaacatcgta tccagctatt
     2281 tggattactt tgacgcttag attatgcgcc aaatggattg cttttttcat tagttgtttc
     2341 gctgcttttt tcttttctgg atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa
     2401 caaatcgaat ggatacgtac accggagctg aacatctctt ctctcagctc agctatcttt
     2461 tcttcagacc aatctaatcg tgctaaccgc tcatccgtct catcaatcga catctcgata
     2521 aaatcgaagt ccatttcttt tacaagggaa aaacgttctt tccatgaaat atctttgggt
     2581 aacgcttttt cataaatccc aatttgagcc atttccttac ccccagatac gaatgatctc
     2641 atctttgaag ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac cacgaccagt
     2701 gataaaggta tacacatcga tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc
     2761 agttacagaa acattaaagc ctagttcaat cagttttttg atcttcgata aatctttttc
     2821 accccaagtt tcaccagata acagcgcatc acggctttga tggtagatta cctgccggat
     2881 accgatgtta tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga gttctacttg
     2941 caattcacct atttcttttt gtgctgcctc catagttgga atcgttgcac aacaaatgac
     3001 tgtcatccag tctgctcctg cttttgcaac attactcgcc actgttcctc cagcgtctgc
     3061 acatttcgta tcagcgacta gttttttatc tgggaacata ctgcgaatac agcgaatcgc
     3121 tttttgtcct tcctgtaagc aaagaattgt gcccacttca ataatatcta ctacatcacc
     3181 aactttcata acatctccta atgcatgttc taagtcattg tgatctaacg caatttgcaa
     3241 attcggcctg ctcatcttag ttccttcctt tacttgtttt tttggtacca ctttgtttca
     3301 gcaaattttt cactgatttc tttgactgac atcacatttt tgataccgat aactgtaact
     3361 ccttttttct ctgcctgagt aaacatattc aaaaaattga ctggcgtaaa aactacatcg
     3421 tattggccag ctgaactttt tccctctgaa atggcacaat gatggatttt ggtaattggt
     3481 acgtttagtt ctttcattgc tttttccaca cttctcatca tcatcaagct tgttcctgat
     3541 ccatttgcac acgatactaa aattctcatc ttaatccctc cattatgagt gttgtctaat
     3601 cagattttct tttatactct ctgcttttct gcatatgtgt catagtcttc tacaatcaaa
     3661 aaataaccat caggattttt ccgatattgt aactgcggaa tagccaataa accaatgaca
     3721 actacggcaa ttccagcata acctaggact ttcatgatta ctgtaaacaa tggccaagct
     3781 gttgcccagt cgaacatccc gatataacca ccgtatgttg acatcccaat ccatgtagcg
     3841 aataatgctg aaccgcctac ttgtatgatt cctgaaagaa acgggaacag gcaagcggct
     3901 ttgaatccac ctcggttatt ggcataaact gctataacgg cattatcaaa gaacaacgga
     3961 atgaaaccgg ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa
     4021 aactgtccca aagcaccaaa caagaaccca atcgttaccg catttggcga accaaagcca
     4081 aaagtagcag ctacgtcaat tcctggaaca gctcctggta aaatcgtgtt tgagattcct
     4141 tggaaagatt gagttagttc atcgacgaat gtccgaacac ctaattgcaa gatcgccaga
     4201 tataccgcaa aatttaaaga agtcgtcata atgtagaata agaaactctg cccttcttgc
     4261 atgaattgcg cttgaatcaa ataatctttg cctagtacaa tcagaatgat cccaaagaaa
     4321 aatagcatga gtaacgaagt agcaaccata ttctcattga aaatcgacaa gaatcctggt
     4381 aatttgatat cttcaatttt tttgtcttgc ttgattgatc tatttttctt ttcatcccgc
     4441 tttttcatcc actcagctaa ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc
     4501 actgcaaatc cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa gttagaagct
     4561 actgtccaat aacaacctaa aatcagtccc ataaaaatca atgcttgtat cctacctaga
     4621 tcaggaaagc aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt
     4681 ccggtagtga aaacggaacg cagtttcgta tatttctgaa aacggactaa taagatattt
     4741 aaaacaaatg caatcagtaa taaaatcatc acatctccaa aggtacggcc aaatgtctcc
     4801 attaatccag catccacagc attttgtcca aaataaggat ctgtaaccat ggcatccaaa
     4861 ttgaaccgtt cctttagtcc aaccaagatt ggccgaaaat tattgaccaa accaccagag
     4921 ccaacagtca aaataaagta accgaccgtt gcctttaaga atccagcgat gcattcatga
     4981 agcgggcgtt tcagcaaaat atagcctaat aacacgataa aaccaatcag gtatgctggc
     5041 tgcgtcaaga tatttgttgc aaaatacgtc caaatgctta acaaaaattc ccccatcttt
     5101 tttctcctct atcaattaat tatattctgc catcacaact tggtaatcat cgatggagtt
     5161 cgtagagatg agacgctcaa ccaatccatc tgtcattaat aattcggata atttttggat
     5221 attttctaga tgttcttctg gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt
     5281 ctcctcattc tccccttcac tttcaaaacg gattggctgt ttcaacttgg taaatgaaat
     5341 agctgttcca aaaacaccat cacttttttc tgaagaatgc ggcattgcta cttgaggtgc
     5401 gatcacaata taaggaccgt atttttctac acaatggatg atctcctcca catacgtttc
     5461 atcaataatt tccttatcga tcaatacaag acagctttcg gcgatagctt cacgccaatc
     5521 cgtgatttct ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa
     5581 actccccttc tttcttataa aaacgacgga aacggtgtat cattttcaat cgagaagaca
     5641 tcttcaaatc ctcgataaaa attaaactca agatcatctt tgtttgtcgg atagatgaat
     5701 ttcccgccta cttgccagat ataaggttta aatccatact tcaggcgatc ctttttgaat
     5761 ttccaaattt cagtgatctc ttttggatca gccataaaat tagaccaaat atcgtagtgg
     5821 acaggaatca ctacttttgc attcaatgac tccgccatgc gcagcatatc aacagaagta
     5881 actttgtctg ttatccctcg tgggttttca ccataggccc ctaaacagac atcgattttg
     5941 tgttcattac catgtttggc aaataaattg gagtaatgtg aatctcctgc atgataaaga
     6001 ttccctcctg atgtttcgaa taaatagttt actgcaattt gatccatctc ctgaggcatt
     6061 tttcctttta atacctcttc tggatcttca caagtgacca acgctgtccg atcaaacgct
     6121 tctaaagcta ctacattgat gtctttgatt ttgactgcat cacctggctt tactgtaatc
     6181 gttttttctt caggaattcc ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg
     6241 aatgttgctt caggacaatt ttgatgaact gctgcggctg tatggatatc tagatgatcg
     6301 gaatggatat gtgtaacgat taaagcgtct acatttttta tcgcaaaagg atcgattacg
     6361 aaaggctgag tacgtaaatt aggctgcata ttttgaacgc cactcatacg catcatctga
     6421 tggccttttt tcatcatgcc attcccatga ctacgttttc ccgtcccaca ccatagatca
     6481 cataaaatat tcgttccttc atgacttttc agccaaattc ccgtacagcc cagccaccac
     6541 atagcaaaag ttccaggatc cactacttct gtttcgattt cttcattcaa gtatgtgccc
     6601 cactcaggaa atgtttctac taaccacttt tcttttgtta cttcattgac agttttactc
     6661 atttgcttgc tccccccata tcgttgttcc aacttacaag taaatattaa tgtgtatcag
     6721 catccatgaa aaccctttta tatgaccgtt tgttttttat atgattttct ttcagtaatc
     6781 aatgattgtt tcagtataat aaagacagat atgaatgttt aaatgaacaa ctagtcaaat
     6841 aaatccatgg atcaagatgt ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat
     6901 attgaaaaac gtcaaaaaga aattttggaa ctgcttcagc agactgaaca actatcaact
     6961 ttcgaattag ctgaatccct tgatgtatct attagtacga tacgaagaga tttaaattta
     7021 ttagaagaaa aaaacgagat catacgcaat tacggttatt gttcttataa ttacaaaaac
     7081 cagactgatt ttgatcaatc tggtcctgaa aggattaaac aagcgattgc cagagaagct
     7141 agtaaataca ttaacgatta tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa
     7201 acactggatt ttcttcaatc taaccatttg acagtcatta caaacaacgt gaaaatcaat
     7261 tactctccgc acaaagataa ttgtagttat attttaactg gtggagagat gcgctttcca
     7321 aaagaagcac tagtgggcga catcgcatta aatacgattt cttcagtcaa tgcagacatt
     7381 tgtatcattg gctgtagcgg agtcgatgta gtacatgggg tcacaacaaa gatattaaat
     7441 gaatcaacga tcaacgaaat gatggtcaac aagacaatca aatgtaagat tttagtcgct
     7501 gatcatcgaa aaatcggact gacatcaaag ttcaagattg ctgatatttc agtctttgat
     7561 tatttgatca ctgataaatt ttgttctgaa aaagtattag aggagatcag aaatacgggg
     7621 ataaaaacca ttcaagtaga ttcattttaa gaaccaggac gtaaatatgt aaataggatt
     7681 atcaaaaaaa tgaataacaa atttatcgat aaatgaaaaa cacgctacgc tcaaattgat
     7741 aaaggaatcc caagatgtat ccatccaatc gataccatca taatctattg cttcttttat
     7801 aaccgttata tgttgcctaa ataggaatca aacaatatcg aaaaaaggag gtatgacaaa
     7861 agtcacacct ccttttttcg aataaacggt attcaaatca gaacactgat ttcacaacca
     7921 ctttatttca ggtcgttttc aatcaccttt accgcgtaat catttccgtt gaaacgcgct
     7981 attttttttg cgtccatata tctttctaaa taatcatctt ctcctaattg cttatggcaa
     8041 gaagcaattt cataaagcaa gtattccaat gcataagtcg atttatgttt cttattatac
     8101 tcaataccct tctcacataa ttggattgct ttatcataat cctcgatttt acggtaatag
     8161 gtggcgatat cataatatac aatgcagacc atcagattat caaaatcagt ctcactcaat
     8221 attttaagtg tacgctccac ataaactttc gcaaaatcta attctccttt tgacaagtag
     8281 atcattgcca cgtttgcagc tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc
     8341 aacacttgat taaaataaaa taatgctgca tctggttttt tcgcataaag atgatcaagc
     8401 agtccttgat aaaataaaat ttgatttaaa agatcagaat tacttacttt ttcctgatcg
     8461 atcgtttgta aaattttata agattcttgg tattttgata cagaattcaa tgattgaatc
     8521 tcatttattt ttttcctgat tttgtcttct tctgtttcta ctaatatatc ggagatctcg
     8581 acattcagct ttttgcaaag cgcacttaag atattgatgc ctggcgacat attcttgttt
     8641 tcgatattgc tgacagttgc ttgttcacaa atgccttctg ctaattgtac ttgtgttaac
     8701 ccctttttct ttcggtattc ttttagtaat gctccatcta ctttaatcaa aacaatttcc
     8761 ttccctttaa aaaattattt ctcctttata tcatacacaa aaaaaagttc taaacaacca
     8821 aaaatgttta gaacagaata aaaattttaa ttttaaaata atattcaaat taaacaactg
     8881 gcgtatctgc tccttgattt ctttttgtgt tatctgaata attatcgttt acagttataa
     8941 cacatagtga aaattgtcat ggcgagaaag tctaagaata tttttaaaca taactatttc
     9001 tttcttacgt gtgtataatg acgacagcac acctatcaat taatataatt attttatttt
     9061 tttatgttct catgtttatt tttaatgaca caacttctcg tatttatctg gcacaacata
     9121 taaatcgatc aaaaaattgt tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca
     9181 aaatttaaat ttagaagttg tgaaaacagc gagagacagt ttaatattca aaatatacat
     9241 aggctactcg aacgctgtgt tttttagtat actagtaaga aacgaaggga tagactcatt
     9301 ccaataagga ggatttcatg aagacaattc tttttgctcc tgcagtattc aatttagctg
     9361 agacaacacg aatgattgag gttgccaaag ctatgaaaga ccgttattac tgtgtattct
     9421 ttggcttttc taaaactttt gctcctttga tcacagaaag tggctttgag tatcatttac
     9481 tagcgccgat ccttacgaaa aaacaagaag aacaaatcat aaaatttgac cagttgcgta
     9541 cacttaaaaa cccttttacc aaagaaatgg ttgcctctcg tatcactagt gaacagagtc
     9601 tgattgctaa gtttgagcct cgactgattg tgacaggttc gaatgtgaca atttttttat
     9661 cggcccgcaa taaaaagatt cctttagtat atgttaaacc ctatgctttg agtgaacctt
     9721 tttttcaatc agagcatgcg ctgatgcccc acgagttacg tttcttcggc cctttaagca
     9781 atcccctttg gcactggaca aaaaagcgta tagtaaatac ccgatggatc cccaatgcct
     9841 tcaaacacgc tgcacaaaaa gaaatgcttc ctttaccaaa aacaagttta caaatgatgg
     9901 atgctgatct aaatctcatc acaacgccta aactattttt ggaagaagaa cagctgccgg
     9961 aaaattatcg aatcgttggt ccaatttttg ctaagctcag tacttccttg cctgaagaag
    10021 tgctaacttt tatacagcga aagcaacaag aaagaaaacg tattatctat ttcgcaatgg
    10081 gtagttcagg caatccgcgt atgttacgca aaattttgtt cttcttacaa aaacaaccgg
    10141 atctagcggt tattgcacct attgactatc tgcttgatga cattcaatct gactcagaag
    10201 atcttttaat aacagaatat ctgtctagtc atcttttgca tgaattcatt gatttttctt
    10261 ttatccacgg cggggaaggg accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg
    10321 gtgtggggat gcaatttgag caaagctgta atattgacta ttgtgtaaaa tacggaaacg
    10381 caatcgccct cacaaaacca gtgactgaaa gaaaactgca gattgctctg cgcgaagttt
    10441 cttcacccaa gattcgtcag caggcacgca cgctgcaagc cgtttttcca ataaatggtc
    10501 cacaacaagc aattcaagaa atcgaaacct tcctgagcaa ccacccaata tagtttcaag
    10561 tagaaaaagc ttttttaata gagactcatc agtaatttcg ctattcgaga cccagacagt
    10621 gaaaatacaa aaaaagtaaa tagtggattg aaaagttaga gttacttaaa ttacggtgtc
    10681 taaaaaacac aaaatcatcc ttaaaataaa agatctgacg ctatccaaat gagcataggt
    10741 agcgtctgcc atttattctt agtcaaattc tttgacacct ttctctggtt ggatcatctc
    10801 aacgtttctt caaaagaaag accataataa ttaggcaaat acattttctt aaaatgtgtc
    10861 atatagatct agaagcatcg ctttgtagag atttgtgccg ctagtcccct caacaattac
    10921 taaatcaaat gtaggattta ccactttatc tttgataatc aacctccctc ttctgttgag
    10981 acaatgatct cgcaatccga tgcatactcc tatataaact gccagcaatc cccacatatt
    11041 gagtagatca tgtcagaatg ttctatgtcg tcagcaacga tacactaaaa gtcacgttca
    11101 ccatgactga ttgctattcc cagcacaatt atttctatac acgtttcatg atttgaataa
    11161 acactcacat catacatatt accattcata caccatactg cagcccctac tatatggttg
    11221 taagctccca aatctatatt tgacttgatc actttggtag tagatctact aattctaaat
    11281 ccattgctga taaaatttcc attttttcct ccttctttct gccaaaacag ttaaaacaat
    11341 aataatcttt atgtaaaaaa gggctgtgac aaaagccttg tcacagtccc atattccgaa
    11401 taaacggtgg tcaaaagctt gcatctgcgg taataagttc aaccaaacga aaaataagcc
    11461 aacatttcac aaaaatttaa gaagcaattt tcgtaaattt cttcttattt cttgaagcta
    11521 accacttctg ttacaaccac ttttagaaca tttaaagctt cgatgtgaca aaccatctat
    11581 gctgctcgat cataaaattg atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa
    11641 acaaaacatt catatgcggt acaatgatct ttggaagaat cacgatcaaa aatgatgcca
    11701 gtgcccaaat ccatcgattg atttgaatat gatatgtagc tagcatattc aatataaggt
    11761 gactagccgt taataaaaag ataaaaacaa aaaataaaat cagccaatac attgtttccc
    11821 accttctaaa aaccaaactt agacagtctg gacttcgttt tctccacgac tttgttcatc
    11881 tgacagcatc tttttatcat aagcttttac aaatggataa tagataataa atgctatcaa
    11941 tgtgttgatc atgatcaaga taacaacacg ccagtcattt cctgctgaaa gataaccgcc
    12001 gattggagct ggtaatgtcc atgttggcaa aacagtaaat ctattgacca tttctaattt
    12061 gactgcaaaa taactgataa tagtcaaaac taaaggaact aagttgaacg ggatgaacat
    12121 gtaaggattc aacatcatag gagctccaaa aattatgggc tcattgatat tgaagatacc
    12181 aggaataata gaaaaacgac caacctcttt caagaatttt gatttacaga agaatataaa
    12241 caaaacagat accacgatcg ttgctcctgc accaccaatt gttaccgtcc attgataaaa
    12301 ctgttcagga gcgatatagg gtaattgggt ggcagctgta ccatctgcca atgcctttgc
    12361 attctcatcc aacatgatca gccacattgg acgaacaaca gcccctacaa tactcatgcc
    12421 atggatacca aatgaccaca acaaatggat caaaaacatt ggtagtaaaa caccaagcaa
    12481 attattccct aaaatattgg ttaaaggctc gaataacgat aataaggcag cgtttacatc
    12541 aaagcccgcg acgcttcgaa tcagccaagt aattgttaaa atgactgcac ctggaatcaa
    12601 tgcctcaaaa ctacgggcaa cagcaggtgg cacttgttct ggcattttga tagtaatatt
    12661 tcgattttta aagaaacgat atacttccac tgcaaaaatc atagataaga tccccgcaaa
    12721 catcccagat gctcccaaag aactcatagg taagacccag ccaagaggtg cctcttcagg
    12781 agttaaaacg tttacaggaa cttgtaacat aaagaacgtt cctaaagata atataccacc
    12841 tgtcacacta tctaatccat atgatttagc tagatccgaa cccataacaa aagttgcata
    12901 aatagccatc aatcccatcg tcatcctaaa gggtaaaaca atcgttgccg aataaggggc
    12961 aataaattca ttccatccag gaaacggtaa attgataaag accataaaaa atgtcccaat
    13021 caaaatcaat gacaatgttg aaacaacccc attacgaatt gctaataaat gacgctgtgt
    13081 accaattttt gccattggtg ggacaagttt ctcttcgacc catttcatca gcttttccac
    13141 taaaaccact ccttttcaat aaaatatgaa ccattcattg ca
//
LOCUS       DAYYAR010000080        12937 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000080 DAYYAR010000000
VERSION     DAYYAR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.080"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1194)
                     /locus_tag="ACHAT0_001893"
     CDS             complement(46..1194)
                     /locus_tag="ACHAT0_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8451825.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(1316..1912)
                     /locus_tag="ACHAT0_001894"
     CDS             complement(1316..1912)
                     /locus_tag="ACHAT0_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8451826.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2112..4754)
                     /gene="alaS"
                     /locus_tag="ACHAT0_001895"
     CDS             complement(2112..4754)
                     /gene="alaS"
                     /locus_tag="ACHAT0_001895"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8451827.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5142..5573
                     /locus_tag="ACHAT0_001896"
     CDS             5142..5573
                     /locus_tag="ACHAT0_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8451828.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5618..6967)
                     /locus_tag="ACHAT0_001897"
     CDS             complement(5618..6967)
                     /locus_tag="ACHAT0_001897"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8451829.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7037..8200)
                     /locus_tag="ACHAT0_001898"
     CDS             complement(7037..8200)
                     /locus_tag="ACHAT0_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8451830.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8329..8652
                     /locus_tag="ACHAT0_001899"
     CDS             8329..8652
                     /locus_tag="ACHAT0_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451831.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8835..10184)
                     /locus_tag="ACHAT0_001900"
     CDS             complement(8835..10184)
                     /locus_tag="ACHAT0_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8451832.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10253..11206)
                     /locus_tag="ACHAT0_001901"
     CDS             complement(10253..11206)
                     /locus_tag="ACHAT0_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8451833.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11308..12498)
                     /locus_tag="ACHAT0_001902"
     CDS             complement(11308..12498)
                     /locus_tag="ACHAT0_001902"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8451834.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(12698..>12937)
                     /locus_tag="ACHAT0_001903"
     CDS             complement(12698..>12937)
                     /locus_tag="ACHAT0_001903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451835.1"
                     /translation="KNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVFL
                     LVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         3721 a         2718 c         2230 g         4268 t
ORIGIN      
        1 ctctttaaat taaaaacttt aaggagtctt tttatagttt ctcatttaat ataaaagata
       61 attgtgatca aacgtaattt ccaattggta atctccgatt ttttcttcta tttgactacg
      121 taagattctt tctagttctg tatcaacaaa ctggaatttc tcagggacca ctacatcaaa
      181 ttgcagcgtt tttccttgat gaattactct aaaatcatga atattaagcc ctgtacctgt
      241 ttcaagaaga atttgcttta actgccgcaa gatattatag tatttttcgt tatcaacagg
      301 cattggatcc aagtgacaga caagttccac atccaaagac tttttaaagt ctttttctat
      361 cagatcaata atcttatgag ccacattcaa attcattgat tcatctactt cgatgtggac
      421 tgatgcaaag cgtttattgg gtccatagtt atgcaccaat aaatcatgat agccaagtat
      481 atcatcgcaa gcatttaaat gctcttccat ttcttcaatc tctgctggcg ttgggcgact
      541 tcctaatagt tcgtatacaa aatttcgaag catcgtcaat ccactataaa ggatatataa
      601 tgctaaagca aaacctatat atccatcaat acgccatcca gttacccatt cgattgctgc
      661 tgaaagcaat acagccagtg tagtaaagac atcattataa ctatctgttg ctgtagctct
      721 caatgtttca gaatcaatgg attgcgcaat aacctgatac attttgcctt gtgcaaattt
      781 caaaaagatg gaaatgatta ggacaaaaaa gacgatgggt gttaaagaga cattttctgg
      841 atggatgatt ttttcagcag aagactctaa aaattgaaat cctacataag taatgatgat
      901 cgaaacaaat aacccactga tatattcgaa gcgttcgtgt ccataaggat gttcctgatc
      961 tgctggcttt gcagcaattt taaatccgat caaagtcaat atggatgaag cagtatctga
     1021 tagactgttc actgcatcag ccataatgga gacacttcct gaaaaaagcc cgatcattag
     1081 cttagagaca aacaagatca gattcgtaac caatcctaag atacccgcta gtactccttt
     1141 ttttgtccgc tgttgcggta tttgtttctt tttttcttct tgtttcttag ccaagttttt
     1201 taccccttct agtttgcttg ttgctcaggt aaaaagatgg agaggacgat tgttttcctc
     1261 tcctctcttc ctttttctat ctatcgtgcg tcctattact attaaaagat ggtcattatt
     1321 ctggtttcaa cggtgttgct gcaaataacg cttttgctag taatggtgtt atgattcccg
     1381 cacctattgc ttctggtatc ccattgattg ctgcaacccc tgctaaggtt ttaaataaaa
     1441 gagcagagtc tacaccatag gcatctgccg ttgcacctgt ataaaataat cccatgaatc
     1501 ctaacactaa gactgtattg accaatgaac ccagtacccc tgcaaaggct gagctgatcg
     1561 ttattgattt atttttttga taaatccagt ggaaaaccaa gcctgctact aagccaacaa
     1621 gtactctggg aactaccgag ataatcggat tcgtgaacac taatgtatca aatggcgttg
     1681 ttggcatggc ataggcacga acaattgtag cgattcccca aaacaagcca atcaccatgc
     1741 catcttttgg gcctaatact actgcagcaa taattactgt gatatggata attgttaaac
     1801 taatgaatcc taatggaata aaccccagcc aaggaaccat cgcttgtacg atgatgattg
     1861 ctaacaagat cgcacgtaag actagtcgaa aggtcttatt tctactattc attttttcac
     1921 ccctttttat ttatactaat cgtaaaaatt tctgtcactt tcgtcaacta aagaccatcc
     1981 gaatccgacc atttgattag atgcgccatc caattgaaaa aagtatgagg gcaagacttg
     2041 tttgtcttcg cccccataat catgtttcgt tcaatgtttg aacttttgtt tttctgtcaa
     2101 acgtaaacgc cctattgttg cgcaacccat tctttcgcag ctttaagtgc atctggaata
     2161 ccagcaggat ttttcccgcc agcttgtgcc atgtctggac gtccaccacc gccaccgcct
     2221 actttcggtg cgattgcttt aatcaagtcg ccagctttta gtccagcttc atttgattct
     2281 ttcgtcattg ccgctagcag actgactttt ccttcttgtt cgtttgctaa aacaagaata
     2341 tctgaaatat ttttttgttt ccattggtca gctaattgac gcagctgatt catatctttc
     2401 acttttactt gtgctgcaat gattgttttt ccattgatcg tttctggatc tttgaataca
     2461 tcaccagctt gttgattcgc taacttgcta gctaattgtt cattttcctt ttgtagctct
     2521 cttacttgct gttgcaattg ttccgctttt gaaacaactt ctttcaattg aggtgattta
     2581 acaattgttg ctacttgttt tagttgtttt tcttcttctt caagtagttc gtacgcttct
     2641 ttacttgtta cagcctcgat tcgtcgcacg cctgcaccaa ttcctgattc agaaacgatt
     2701 ttgaagattc cgatgttttc tgtatttgct acatgtgtgc ctccgcacag ttcgattgac
     2761 cagccgccaa tatttactac tcgaacgtca tgtccgtatt tttcaccaaa caaagccatt
     2821 gcacccatgt ttttggctgt atcgatatct gtttcaattg tttcaactgg gatagcttcc
     2881 cagatttttt cgttgacgat tctttccatt tcagctaatt catctgaagt gatttgacca
     2941 aagtgggtaa agtcgaatcg taaatgtcca ggagcgacta aagaaccagc ttgatttgca
     3001 tgctcgccaa gtacttcttt caatgccttg tgcagtagat gggttgctgt atggtttttg
     3061 atgatccgat tgcgcatcga ttgatcgatc aatagatgat aggtttgtcc ttcactcatt
     3121 ggtgctttta cttctacggt gtgcataaat tgaccattag gcgctttttg aacatctgta
     3181 acaactgcta cgatttcgcc tacttcattc atgatccatc cttgatccgc tacttgccca
     3241 cccatttctg catagaaagg tgtttgatca aagatcattt gagcttcacc ttctgcgact
     3301 tcattcaaaa gagactcacc tttaacgatg accaataatt gactatctga ttcttgttct
     3361 gtgtatccaa tgaatttact ttctactttg atatctgtca ataaagcaga ttgtaccccc
     3421 attgattttt cagtactgcg ggcagagcga gcacgttcgc gttgtgcatc catttctttt
     3481 tcaaaaccag catgatcaac tttcagtcct tcatcttctg ccacctcttc cgttaattct
     3541 accgggaagc catatgtgtc gtatagtttg aagatgtctt ttcctgctag agtcgtcccg
     3601 ttttcgtttt ttacttcttg gatcacttga tttaagattt ccaaaccttc attgattgtt
     3661 tcattgaaac gttcttcttc tgtacggata actttttcaa taaattcttt ttgttgaagt
     3721 acttcaggat aataacttac catgatttcc ccaacaacag ggactaaacg atataagaat
     3781 gcttgttcaa tacctaattt tttcccatgc atgacagcac gacgaagcaa acggcgtaag
     3841 acataccctc tcccttcatt cgatggcaat gcaccgtcgc caatagcaaa tgaaagggcg
     3901 cgaatatgat ctgcgattac tttgtaagaa atatctgtta ctgggttttc accatatttt
     3961 acttgtttgc ttagcttttc tacggcgtgg ataataggca taaacaagtc tgtctcaaag
     4021 ttcgttgacg catcttgaac gattgagacc atgcgttcca atcccatccc cgtatcaatg
     4081 tttttatgag gaagtggttc aaatgtgtta tcaggcttat ggttgaattc tgaaaatact
     4141 aagttccaga tttctaaata acgttcattt tctcctcctg gatagttttc aggatcgtct
     4201 tctgctaaat cgttataagc ttctccacgg tcatagaaaa tttccgtatc cggaccacat
     4261 gggcctgcac cgatatccca gaagttgtct tctacatcaa taatgtgatc tagtggcagg
     4321 ccaacttctt catgccagat gcgttttgct tctgtatctt ttggataaac tgttacataa
     4381 agtttttctg gatcgaatgc catccatttt ggatcagtca aaaattccca tgcccagtgg
     4441 atggcttcat ttttgaaata gtcaccaatc gaaaaattac ccagcatctc aaacatcgta
     4501 tggtgccgtg ctgttttacc gacattttcg atatcatttg ttcgaataga tttttgggca
     4561 tttgtgattc ttggattttc tggcacaact gaaccatcaa aatatttttt caatgttgca
     4621 acaccagagt tgatccacaa taaggttgga tcgttcacgg ggatcaaaga cgcactaggt
     4681 tcaatcgtat gtcctttaga ttggaagaaa tcaagaaaca tttgtcgaac ttgtgcacta
     4741 cttaattgtt tcatgtcatt acctgctttc tataaattaa atacaaaaaa acaccatcac
     4801 agccaaggac gaaaactcgc ggtaccacct tgattgcaat gatgtgtaat cattacctct
     4861 taaactcgat aacgctgagc tgcgtcggta tttctaccaa aatagaaaaa gggagcaatt
     4921 tgattagtga ctattctttt ttcagccaag aaagaatttt ctgtgagtct taaaaaacaa
     4981 atcatatcct tttcgtactt aattagtata cgcattctat tgatcactgt caatatctca
     5041 atttttattt tcatttcctt ttggtcatac tccacgattt ttggtacgat aagaataaag
     5101 gaatcaatac atctctgcta ttcatgacaa ggaggaatat gatgaaaaaa atagttggag
     5161 cggatgaatg gatacgcgca gctagctgct ttttgagata tcaagttttt gtattagaac
     5221 aagggattcc tgcagaacta gaattcgacg gaaaagatat cccgcaacaa acctattatc
     5281 tatttcttga tcaaaaaaga ccagtagcta ctttgcgtta tgaaaagaaa acacctactt
     5341 gcttcaagcc ggaacgattt tgcgtagcaa aagaataccg aggaaaaggg ttaggcggtc
     5401 agttgcttga agctgctgaa aaacgggcgg tttcagaagg ttgtcagact agttatgtag
     5461 tagctgaaat gacggctgta gatttttatg aaaaacaagg gtatcatact tgcacagaac
     5521 cttttattga agatgaagta ctttgtatcg gaatgaaaaa aagtctagct taaaaacaag
     5581 agattgtgac acgccttttg tcacaacctc ttgtttttta tttatgtgaa tttttcttat
     5641 ttttacgagc agtacggctt tgctgtctgc gttcgatttt gcgttctttt ttattttttt
     5701 ctgcaattgc ccaatcgatt tttttcttat aaccaggctt gatctttttc tttttcttct
     5761 tcacaagacc aatcagcgtg ggatctaata catcttttga tttttctcgt ttggcacgtc
     5821 tattacgatc gtatgttttg acgatttccc cgtttttgat ttcttttggt tcaaatgaga
     5881 cgcccaattt ttctatcgca tcaatggctt gctcatcatt tggcgaatat aacgtaatgg
     5941 ccgttccttt taagccatta cgtccagtac gtccgacacg atggataaag aagtctagat
     6001 cttctggaat ttctgcatta ataacatgag agacaccttc gatatcgatc cctctagcag
     6061 ctaaatcagt cgctacaaca tattgatact ccagattctg tacttgtcgc atcactcgtt
     6121 ttctctctct tggggtgatg tctccatgaa tctttgctac tttcaagcct tggtttttca
     6181 agtaatcggc aatttcatcc acatgctgtt ttgtattggc aaatacgatt gctaaataag
     6241 gatgaccaat tgtcagcagt tgataaatca tttgattctc atctttacct tttgttgaaa
     6301 tcagccaatt ttcaatgtct tcagaaataa ctgcttttgg tttgatttct tcaatgattg
     6361 gattttccat gtattttcgt aagaatggac gaagtttttc tggaatagtg gcagaaaaaa
     6421 ctaaaaactg cagcttttct ggtaatcgac ttgcaatttg gtctacttca tttaaaaaac
     6481 ccatatccaa tgtcatatcc gcttcatcga caacaaatgc aaatgcggtg tgaatcttca
     6541 gcgcttgttc attcatcatg tctaatattc taccaggcgt gcctactaca acctgtggct
     6601 gttggtgatg cagacgattt aattgacgct gtttgtctgt accgccgaca aaattcgaga
     6661 cacggatctc cggtgtagaa aatttagcga tttgtgtagc tgcttcatag atctgtgcag
     6721 ctagttccct acttggagct gtaataacaa cttgtacttc ctctttttct gcattgattt
     6781 tttcaaacaa aggcaacaaa aacgtatgtg tttttcctgt accagtttga gattgcccaa
     6841 tgatattttt tccttttgca attaaaggaa tcaaacgttc ctggacctct gtgggctgtt
     6901 tgaagccctt tgcggttaac gcttcattga taaacggttt gaagcggaat atatcaaaat
     6961 tattcattcg tgcacctctt ctctttaaat tatcacgact ttggcattat aacacagaca
     7021 aagcgttttt gctcagctaa tttcgagaat catcatcctt tgtttggcca atcgtgctaa
     7081 cagtatatgt gtgactagct ttttcataaa acagaatccg tttttttctc aaaaatgata
     7141 gcaagatatt taaaataaaa ataccttgta gtaacaagac accgagagaa atgaatactg
     7201 cactcatctg caacaccgta ttcgttgtcg aattcatgat ttgctgaagc aactgcccca
     7261 accagcttaa accataaaaa agaaagtaca gatacccgta gcggatcatc aatgctttta
     7321 aagaaattcg ttcgcggtct tcttccacca aacggatacg cacaattttt ttgccaagtg
     7381 tccatccatt ggttataaac ggtacaatca taaagtatac ccctacttct aataaaagcc
     7441 accagatatg agaagtaact gcccctgaat cgaacgcaat tttaaagaaa atcgccgcta
     7501 ccaaattaat aagagataaa aatgtccagt cgatcaaata agcgaccaag cgtcgagtca
     7561 atgagacagt ctttcctttt tcataagaaa gttcatccat tcgtcttctt gtaggaagca
     7621 taaatgtaaa taacggcgta acgacaaaac caatgattcc acccaatgta tttgtcacta
     7681 aatcattcac atcaaataag cggtatggtc ttgggtagat aaaataaagt ccacttagct
     7741 gcgtcaattc aaaaaataga gaaaggcaga aactagcaat catcgttttc ataaaagaaa
     7801 gccggaaata atatcttaaa taaatgccaa acggtatgag taataaaaca ttaaaagctg
     7861 gttccaaaaa tacactctgc ttcattgccg gcaggtaggt actaggttca gacaagacca
     7921 ataccgtgtt gtctctgaaa tgttgccatg aagcaaataa atgcagctcc atctttggcc
     7981 ctgtcatttg ggcaaccgtc tcctttgccg gaagtggtaa gatcaccaaa aaatacgcac
     8041 acaataaata aaaaacgaaa gaatacaaaa taactgccct tgttactaga aatgttccat
     8101 actttcgata ttcataaata aaaaaaacag atgagatcgc taaggctaaa aatggaaaaa
     8161 caatcagcgc aaaacgaatc ggttcgatat aacttgccat atgactcctc cttgattctt
     8221 tgtatctctc ttatcttagc agtcctttgc tgtttcaagc aagtaacgag gctattattt
     8281 ctcatttctt tcaaagtaaa gtaagataaa cacaaagggg gtgtcgagat gctgaatatg
     8341 tactttgttt ttggcgtacc tattttttta ctgttccttt atgctacgat cgcttatgta
     8401 agaaaaagaa ctacaattca ttatttagga tttattctcc tgattatttc gggtttcatg
     8461 ctagtcttca atcttcagac ttggcagcaa gcacttcttg aaatggataa aatgactcca
     8521 catgctttgt ctaaagtgct tggctaccct gtctatctga tttggcttcc cattttcatt
     8581 tctggttgct tggttttatt aaatatttat cgcggcgtac gccgaatagt tcagttacgc
     8641 aaatcaaagt aaaagacaga aatcggttga gcccgatttc tgtcttttac tttgctattt
     8701 catcgtagac aaaacgacat ttacgtgatc aaaattaacg agtacttttg acatttcttc
     8761 cacgtactcc tcatttaaat aaaatatttt gtttggcaga gaagtgcagt tctaataaaa
     8821 ttcgaatgtt ttagctatct acgttttaaa tagagataaa tcgggatacc agcaagtgtc
     8881 aatactatcc ccatcgaagc taaaaaggtc tgagtgataa gcgtgcttga tacgataaaa
     8941 gtccctccaa tgattgctac tagtggaata aacggataca ttggaacttt gtatggtcga
     9001 aacaaatcag gttctttttt acgaagaaga atgacaccaa caaagaccat cgtataaaaa
     9061 atccaaatga caaagatcag catatccgtt agtgtatcaa aaccaccgat catcatcatg
     9121 ccaatagcga tgactagctc taagatacct gcggctacag gaactttagt tttatcatga
     9181 agtttagcaa agagcttgct aaaaggcagg ttgttttctt ttgccataac ataagggaga
     9241 cgcattccag taagggtata gccattgatg gttccataaa cagaaatcaa gataccaact
     9301 gtgaccagtc ttccaccgaa gccgccaaaa atcatttttg caacgtcact tgccgcattg
     9361 ctgtttcctg cgactccgtc gatagatgcg gttcttaaga acaccgcatt tactaataaa
     9421 taaacgatca tgattccgat gattcctaaa gaaattgctt ttggcaaatc tttcgccggc
     9481 tttttcaatt ctcccgaaat atttcctacg tgtatccaac cgtcgtatgc gaacatcgtt
     9541 gctagcaagc ccgctcccaa ggctgagaaa aatgaaagat tttctcctgc ttggatcgga
     9601 aacagttgga agtctactcc ttcttgtcgg aacaaaccga aaatgacgat cacaaacaaa
     9661 ggaataagct tgcagacaag cgtaatcgac tgaaaagccc ctccagcttt agaacctaaa
     9721 aaattgatca acaggatcga tacagcagct gtaacagcta cgggaacaat cattgactgg
     9781 gaaagaccaa atagattgac gaactgtgta ccgaaaatga tcgacaaggc tgctacattt
     9841 gctggaaaat aaatcacgac ttgcgcccag cccaataaaa aagctgctgt atttccgtaa
     9901 atccgttcga tatatttaat catccctcct gtttctggaa tagcagctgc cagttcagct
     9961 cctgttagtc ctgcacagac agaaatcata ccaccgagaa accaagaaaa catatgcaaa
    10021 ctggcggatc ctgtaacttc tgctacgctt gcggctttaa aaaatacccc tgcaccgatc
    10081 actgtgccca taacagtcga taaagcagta aaaaatccca tcgtacgttt taattcattt
    10141 tctattttta cttcttttac tggcgtggct tgatattctt ccatcccttg tcccctttca
    10201 taccatagcc aatcccaaaa gtataaaaaa ctttttggat gtggttcttg ttttataccg
    10261 tctctaccaa tttttctttc agtttttcca acatatcctt tgtcatcaca tctaagtcgt
    10321 atttcggcga aaagccccat tcctcttgcg cacaagaaac atctagtgaa tttggccaag
    10381 attctgctat tgactgacgt acaggatcca catcataatc catttcaaaa tctggtatga
    10441 ttttttgaat agatgctttg atctcttccg ggtcaaaaga catcgccgat atattgaagg
    10501 catttcgatg aaccaatcga tcaggatctg cattcatgag ttttataatg gcatcgatcg
    10561 catctggcat atacatcata tccattgatg taccttgatc aatgaaacta gtgtactttc
    10621 cttctttgat tgctgaataa taaatatcga ctgcgtagtc tgtcgtaccg cctcctggca
    10681 aggttgcata agaaatcaat ccagggaaac ggacaccacg agtatctacg ccatatttca
    10741 cataataata atcacatagt agttctccag ctaccttcgt taccccatac atggttgtag
    10801 gacgctgaag agtatcttgt ggggtatgat cttttggagt atttggacca aacgcgccga
    10861 ttgaacttgg cgtaaaaaat ttcagctgga attctctagc gacttccaat gcattgacca
    10921 gcccagtcac gttcaaattc caggctaatt gtggttttgc ttctgcaaca gctgacaaaa
    10981 gagcagccaa atgaatcaat gtgtccactt gatattgttc tgctatgctg cgcatttttt
    11041 cgtaatccat tacatctagg atctcaaaga tatcgttttt ctcaatgact tcaggcatgc
    11101 gtatatctgt cgcaatgaca ttttcactgc ctagttcttg acgtagccgt gtgatcaact
    11161 ctgaaccaat ttgtcctaaa catcctgtca ccaatacttt tttcatgatg atcctccctg
    11221 atctttttta ctactatctt cagatgatta acaatatatg cgtgaacatg ttctctaatt
    11281 ttaaatagac gaaaaaacta aatttagtta aataatttcc atttcttttc ctattttttg
    11341 ataaactgct actgcttcat caagcatttc cctcgtatgt tccgcagtcg gcatatttct
    11401 gatcctgcct gtcccaagag gcacagttgg ataaacgatt ggtttagcat aaatcccatg
    11461 ttcgatcagc ttcttagaga aagtctgagt cactttttcg tctcccaaaa tgactggagt
    11521 aataggtgtt tcagacatac cgatgttata gcctactttt ttcaattctt ctttaaaata
    11581 attagcattt tcccaaactt tttcgacaag ttccggatgt tcttgcatca aagtaatcga
    11641 agccaatgca gctgcagctg cacctggagt tagcgaagta gaaaatagga atggccgact
    11701 tctagctttc aaccagtcga ttagtgtttt cgatccagct acataacccc caacaacacc
    11761 tatagctttc gataacgtgc ccatttgcat atcgattttg tcgcttaatc caaaatgctt
    11821 gacggttcct gcaccttttc cagttacgcc tgagccgtgc gcatcatcga cgtaggtgat
    11881 cagtccatat ttttcaacaa tcggaataat ttccggcaat cgtgcaatgt ctccatccat
    11941 ggagaaaaca ccatctgtga tatacatgat ttttttatat ttcttacttt ctatcgcttc
    12001 ctttgctttt ttctcaaggt ctttcatatc ttgatgttta attcggatga tttttgctcc
    12061 ggataagcgg caaccgtcaa taatagatgc atggtttaat tcatccgata agatagcatc
    12121 ttcttttgtc attactgcag agatagcgcc catattgcag ttgaatccag attgaaaggc
    12181 gattgctgct tctgtccctt tgaattctgc gattttttct tctaattgct gatggatttt
    12241 cagcgaacca ttgatcgttc ggactgctcc tgctcctaca ccataagttt cggttgcttc
    12301 aatacatgct tttttcaatt cttcgcgatt ggcaaatcca agataattgt tggatgctag
    12361 attgatcatc ttttttccgt ctacgatgat acaagcacca ttttcacttt ctagtatgtc
    12421 gatcgtattg tataacccct tttctttcag ttgatccaat tctttctcta aatacattgt
    12481 tagttctttg gaagacattg tctccacctc catattcatc tgtcttgcag aacataccaa
    12541 acatatatcg tgtcattcat ctacaaagca agtatgctat atttttgata acgcttacac
    12601 tgattatgag aattatttaa ccacattttt tatttacagt atctttttac tttcaaatga
    12661 cttatatagt atgaacaatt accttttaga taatgatcta ttttactcta cctgggcaga
    12721 taaatacaac acaaccattg atagaagccc tttcgttaaa atcaattgta ttctgaatat
    12781 ttaatttatt tattgcatag accaataaaa atacataaaa tttccatctt cgtttatcag
    12841 caatttttaa ttttactctc tgaatatttc ttaaaagttt tacttgttga attcgaaaaa
    12901 aggactgtga caaaagtctt gtcacagtcc tattctt
//
LOCUS       DAYYAR010000081        12854 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000081 DAYYAR010000000
VERSION     DAYYAR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.081"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..2225
                     /gene="acm"
                     /locus_tag="ACHAT0_001904"
     CDS             60..2225
                     /gene="acm"
                     /locus_tag="ACHAT0_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8451836.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2497..2982)
                     /locus_tag="ACHAT0_001905"
     CDS             complement(2497..2982)
                     /locus_tag="ACHAT0_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8451837.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3072..3417
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3462..4103
                     /locus_tag="ACHAT0_001906"
     CDS             3462..4103
                     /locus_tag="ACHAT0_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8451838.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4122..4757
                     /locus_tag="ACHAT0_001907"
     CDS             4122..4757
                     /locus_tag="ACHAT0_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8451839.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4775..5590
                     /locus_tag="ACHAT0_001908"
     CDS             4775..5590
                     /locus_tag="ACHAT0_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8451840.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5909..7345
                     /locus_tag="ACHAT0_001909"
     CDS             5909..7345
                     /locus_tag="ACHAT0_001909"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8451841.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7534..9981
                     /locus_tag="ACHAT0_001910"
     CDS             7534..9981
                     /locus_tag="ACHAT0_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8451842.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10211..11278
                     /gene="rlmN"
                     /locus_tag="ACHAT0_001911"
     CDS             10211..11278
                     /gene="rlmN"
                     /locus_tag="ACHAT0_001911"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8451843.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11268..11474
                     /locus_tag="ACHAT0_001912"
     CDS             11268..11474
                     /locus_tag="ACHAT0_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451844.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11471..12574)
                     /locus_tag="ACHAT0_001913"
     CDS             complement(11471..12574)
                     /locus_tag="ACHAT0_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8451845.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4392 a         2157 c         2727 g         3578 t
ORIGIN      
        1 atttttgaga gatgatatag taggttttat ttttagttaa aataggggag atagaaaaaa
       61 tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat ataacatcac
      121 tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt
      181 tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg
      241 acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag
      301 gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg
      361 ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa
      421 acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata
      481 ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa
      541 atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca
      601 tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt
      661 atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa
      721 gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg
      781 gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca gtaactgata
      841 attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt
      901 ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt
      961 ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata
     1021 tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata
     1081 tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac
     1141 agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta
     1201 tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg
     1261 attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg
     1321 gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt
     1381 ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa
     1441 acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag
     1501 tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc
     1561 aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg
     1621 ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg
     1681 aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg
     1741 acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca
     1801 caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg
     1861 tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa
     1921 aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga
     1981 cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag
     2041 gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa
     2101 caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa
     2161 tcattggttt attgatagta actatctgtg ctgggatatt cttacaaaaa tataaaaaag
     2221 tttagttaga atataatatt ttttgttaaa tatgactgaa atcaaaagaa taaaattccg
     2281 atttataatc gaaaaaagaa gcatttttat atatgcttct tttttcgtag aatcaataaa
     2341 tatggatttg gctaactatg gaagaatact cgtattagtt ttcagcgttc atactagttg
     2401 ttacattgtt tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag
     2461 acgcttgaat tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag
     2521 atgttctaag taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt
     2581 attgatattt ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac
     2641 ttggattttt ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac
     2701 gcctaggagg tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg
     2761 ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg
     2821 ttttcctttt cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg
     2881 gacatttttg actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt
     2941 ttttagtagt tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt
     3001 ttttattagt gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg
     3061 taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct
     3121 ctggttggtg gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga
     3181 agcgtatagt aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag
     3241 ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc
     3301 ctttgatctt cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg
     3361 ttttttctgc aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca
     3421 tttgccaaga ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag
     3481 ttatgccgca attattagta ggagcactca caacattgaa attattttta tttaccttga
     3541 ttggctcatt accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc
     3601 tacgctactt gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac
     3661 tgatttttat tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag
     3721 cactcattgc ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga
     3781 tacaatcgat tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc
     3841 aaacggtgac gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga
     3901 atgaagttat caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg
     3961 taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg
     4021 tggcaatcat ctatcttaca ttgactggta ttctaacaat cgtaatgaaa caagttgaag
     4081 gaaaattaaa ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag
     4141 tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg
     4201 ggaaaacatt agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct
     4261 tggctggctt agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc
     4321 cattcgagct taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat
     4381 ttccacatct gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga
     4441 caaaagaaga atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt
     4501 tattgaatat ctatccttat cagctttctg gcggacaaaa acagcgggta gcgattgcca
     4561 gagcattggc tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc
     4621 ctgaattacg ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc
     4681 aaattattgt cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag
     4741 tttccccaat tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc
     4801 tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa
     4861 ttgggcgact atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc
     4921 tatgggattt agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc
     4981 agtctttgaa caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga
     5041 atttgaactg aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc
     5101 aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac
     5161 acctaaagaa tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca
     5221 aaatgggtca tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt
     5281 caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag
     5341 cagacgggtc gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag
     5401 aaacgaacta gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt
     5461 tggtgcacgc aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact
     5521 agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc
     5581 gagaaattaa caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata
     5641 aacagtgttc tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct
     5701 tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta
     5761 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct
     5821 cctttacata attttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga
     5881 ttgaaggtta taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa
     5941 agattttctg tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga
     6001 tgggaaagga gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg
     6061 aacgaacggt gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat
     6121 gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa
     6181 tggacgagga gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt
     6241 gattgaaaac gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt
     6301 gcaagatgaa tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc
     6361 agaaacatta tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca
     6421 aaatatcttc acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc
     6481 gaaacgaatg tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa
     6541 atttcacgag ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta
     6601 tccaattgac agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc
     6661 caattattgg atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt
     6721 agaagaaaaa ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc
     6781 taaaccagat tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt
     6841 aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat cagaagaaac
     6901 tggagttcct ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga
     6961 ctgggagatt gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg
     7021 catgccggta ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa
     7081 acaaatccat gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga
     7141 agcaatttcg gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct
     7201 tagctggctg aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac
     7261 acaagaagga tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat
     7321 tgaagaaaat ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata
     7381 tggccgtgcc ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg
     7441 aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg
     7501 tataatgaaa cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa
     7561 aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg
     7621 attggtgttg ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt
     7681 ttaggaatat tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc
     7741 tgtcttttat tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt
     7801 ggtaaaaacc gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta
     7861 catgcccatt tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg
     7921 gatttgttag ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg
     7981 attggagcaa tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta
     8041 gcagcagtgc tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag
     8101 ctgttaaatg gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct
     8161 gccaagcaag ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg
     8221 aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag
     8281 aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa
     8341 aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat
     8401 cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt
     8461 ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa
     8521 caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg
     8581 gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc
     8641 caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa
     8701 ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat
     8761 atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca
     8821 ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc
     8881 gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct
     8941 ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact
     9001 ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag
     9061 ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac
     9121 ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta
     9181 caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga
     9241 aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat
     9301 ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc
     9361 agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt
     9421 catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca
     9481 aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt
     9541 gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt
     9601 gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc
     9661 gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat
     9721 gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa
     9781 gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc
     9841 ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg
     9901 tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg
     9961 ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata
    10021 aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac
    10081 ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg
    10141 aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag
    10201 ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg
    10261 gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca
    10321 aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt
    10381 atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg
    10441 tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag
    10501 acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac
    10561 gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc
    10621 acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca
    10681 tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt
    10741 gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac
    10801 tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc
    10861 tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag
    10921 tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg
    10981 aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca
    11041 attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa
    11101 tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta
    11161 tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat
    11221 tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc
    11281 gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt
    11341 tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa
    11401 aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt
    11461 tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag
    11521 tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt
    11581 taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga
    11641 gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat
    11701 caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg
    11761 ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg
    11821 ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga
    11881 tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg
    11941 cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc
    12001 tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata
    12061 aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat
    12121 caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt
    12181 cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg
    12241 tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac
    12301 caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa
    12361 tatttccata agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg
    12421 tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca
    12481 ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa
    12541 tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa
    12601 acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga
    12661 acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt
    12721 atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc
    12781 ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa
    12841 gattattata tgaa
//
LOCUS       DAYYAR010000082        12840 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000082 DAYYAR010000000
VERSION     DAYYAR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.082"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..282)
                     /locus_tag="ACHAT0_001914"
     CDS             complement(37..282)
                     /locus_tag="ACHAT0_001914"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451846.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(276..677)
                     /locus_tag="ACHAT0_001915"
     CDS             complement(276..677)
                     /locus_tag="ACHAT0_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8451847.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(864..2672)
                     /gene="uidA"
                     /locus_tag="ACHAT0_001916"
     CDS             complement(864..2672)
                     /gene="uidA"
                     /locus_tag="ACHAT0_001916"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8451848.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2695..4461)
                     /locus_tag="ACHAT0_001917"
     CDS             complement(2695..4461)
                     /locus_tag="ACHAT0_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8451849.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(4474..5268)
                     /locus_tag="ACHAT0_001918"
     CDS             complement(4474..5268)
                     /locus_tag="ACHAT0_001918"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8451850.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(5278..6654)
                     /locus_tag="ACHAT0_001919"
     CDS             complement(5278..6654)
                     /locus_tag="ACHAT0_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8451851.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(6774..7421)
                     /locus_tag="ACHAT0_001920"
     CDS             complement(6774..7421)
                     /locus_tag="ACHAT0_001920"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8451852.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(7505..8932)
                     /gene="uxaC"
                     /locus_tag="ACHAT0_001921"
     CDS             complement(7505..8932)
                     /gene="uxaC"
                     /locus_tag="ACHAT0_001921"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8451853.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(9006..10085)
                     /gene="uxuA"
                     /locus_tag="ACHAT0_001922"
     CDS             complement(9006..10085)
                     /gene="uxuA"
                     /locus_tag="ACHAT0_001922"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8451854.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPACEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(10088..11719)
                     /locus_tag="ACHAT0_001923"
     CDS             complement(10088..11719)
                     /locus_tag="ACHAT0_001923"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8451855.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            12023..12694
                     /locus_tag="ACHAT0_001924"
     CDS             12023..12694
                     /locus_tag="ACHAT0_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8451856.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         4136 a         2537 c         1669 g         4498 t
ORIGIN      
        1 ttttaaacag aaaaagtgga aaggggattt ttttaattag ttaattttaa attttaagat
       61 cgcttctatt aatttaattt ctagctgact tatcatatat tcatctacat aggtaacgaa
      121 ttgacctacc tcgttaaatc catttgtcaa acataactta gccatataag aatgataatg
      181 tttgagtatc atcttgatcg aaattggatc tccttctaga gcagataaga taatggatag
      241 tggaactaat aggtactgcc gctttttaga tttatctacc ataggtcata ccccattttc
      301 aattgttcaa ctgccttctt tcgttgatac cagataccac ttgtgctcat attgagaatt
      361 ttaccgattt cagcatcatt aaaacctgca aaataataaa gtaggataat ctttcttctt
      421 atctccgata actgatcaat tgctgctgat aacttttgat ctgaaattaa tatttccatt
      481 ccaaataaga aatagacatc ttgttcatca aaatgagttt gggaatacct gccttcattt
      541 gaatctgcca atggttcctc attatccttt atgttagcgt ttcgttttcg aatagactta
      601 gcttcgtttc gtaatacttt tttacagtag gcatcaaatt ggtgttgtat agatatgtca
      661 ttgtaatgtt gttgcatgat aatatgctcc tttttccccc tttccactaa tacaacaatc
      721 agaaagcccc tttttttcga aaaaactaga aaaaaaacat aaaaatattt atcattcttt
      781 aaacagaaaa tctttctata aaaaacaaaa aaaaacgatt aggctaggct aatcgttttt
      841 tgaacattct taaaaaatcc aaattatttt ttataactga aattgggtat tgatgaccat
      901 cgtttctgta gatagtaagc aatcatttta ggttttctat ctctagtaaa gattcctttc
      961 ttatttcctt gcactcttaa aatcccttga cttgttgcaa aatctgcaaa attccacact
     1021 tgttcaccaa caaaattttc acatgtatct attacttcgt gatttgcttc gtaataagcc
     1081 acttgatact cttctgtaaa cattgttggt actgtatcat gaaatccagc tactgtatct
     1141 gctccatatt ctgtaaacat tattggcgta tcaggacatc tttccatcca tccatctagc
     1201 tcttttttca acagatattt tgcatgtgcc agatcccctc ccgaaacata ccaaccataa
     1261 taacgattta aacataaaac atcgacaata tctcctacct tacaaacatc tggcgttgat
     1321 tttagataag taacgattgt tacaggtctt ttctgcggat caagttcttt agttaattca
     1381 actaaaggtt caaaatattc tttagcacct tcggaatccg aatctggttc atttgcaata
     1441 gaccacatta caacacttgg gtggttcttg tcgcgttcta tcaattcctt gatcaccgtt
     1501 ttatgagaat tttctgttcg tatctctttc cacgtattat gtttttccac attatcatct
     1561 aataaagtgg ccatgaaatt taaatgtaac cctactgcag gtacttcatc aataactaca
     1621 ataccttctc gatctgctaa tcgcataatt tcttctgagt aaggataatg tgatgttcta
     1681 aaagaattag cacctatcca tttcattaaa ttaaaatctt taatatttac agcctcattc
     1741 aaaccacgtc cattaacata cgaatcctca tgcttaccaa atcctttaaa ataaaaaggt
     1801 ttattattaa tcaaaaattt gccattggta acctgaacac tgcgaatacc aaattcttct
     1861 gtataggtat cgatcacttt attatcatca attaaactaa ttttcatact atataaataa
     1921 gcgtctagag gttcccaaaa aattggattc ttaagctgaa tcattccttt ctttccttca
     1981 gcttcagcta caagttgagc ctcttcgtca tatatcgcta ttaagatttt tccggtccat
     2041 tctgtatcga tattgaattc tacacttgca tccaaatcag ttattcttgt attaatagta
     2101 atatcactta tatagtcttt cggagttgta tataacttaa caggtctatg aatacctgca
     2161 taattgaaga aatcaaaatt aactaagttt tcctttttta tatccccatt ttcctgtact
     2221 gtttccttca taatccccac aggtaaagta ctatcgtcaa taatattgtt tacagcaact
     2281 gtaattcgat tattcccgtc ttttacaaac cgattaattt cagactcaaa tggtgtaaac
     2341 ccacctttgt gttgagtaat aaactttcca ttcaaaaaaa cttttgcttc atgtgttgca
     2401 gatccaaaac gaagaactaa tctttcttct aataatcttt taggaagagt cacttctttt
     2461 tcataccaca cccatccaac atgatttcta atgttctctg tttcaaataa atcattataa
     2521 gaagagggaa ctgaaatcgg ataactctct ttttcattta ttggttctaa agcaagttct
     2581 tcctgtaagt catcatttgt atctaattta aatctccaga gaccgcttaa gtcaattact
     2641 tgcctcgtct ctgtcataat gggatacaac atataaacca ctcctatcta aatattaaat
     2701 aaatgtatcc tttaaaccac aataagcatc tattggactt actcccttaa attcagaaag
     2761 acccattagt ttgtccacca atatttctaa agtttcttgt tttccatcat aagtattaat
     2821 ataagtcttt acttgaggta catcagccaa atgaaacgga cactgtacag atacaaaaat
     2881 tgttggaatt tcatgaatgt aaaacggtat atccggagtt cctttacttg ctggccagac
     2941 aattcgttga actgtgcctg gattaacatt agctatatta atgatcaagt catatttttt
     3001 agttaaattt ttaattggtc tcttagcagc atatattcca gctatatttt taattttttc
     3061 ttcttgcgat aatcctttca attcttcttc aggtgaaatg tatttttcaa cttcaaatcc
     3121 ttttgctgac aaaatctgtt tcaataaatc aatggcttga ggtctatttc cagcaactac
     3181 ttcaccaaac ccacctttta ccccatgtac atctactagt aaaatccttt tatatttttc
     3241 tggtacaata ggccaaatat cttgtttatc tttaactaat gtaattgctt ggtcagccac
     3301 ctctttaaaa attgatttat tttcaattaa accaattttt tccagagctt cttgcttagg
     3361 taataaaatt tgttcttttg cttttttgtg taatcccaat ttagctttta accctaaaat
     3421 tctttttaaa gcatctaata accgatcatc agtgataatt cctttacgat agccttccat
     3481 catataatta aagtcttcat ctggatcatt aaaaaataga aatagatcac atccagctgc
     3541 aatagaagtg ggcaataatt ctgatcgttt cattgcccca gtcattccta acatatgact
     3601 agcatcagta actactaatc cattaaatcc gagttttcct ctaagtaagt cagtgattaa
     3661 ttcttttgat aaagttgcag gaagtaaatc ttcatctttc agatcaggat taaaatgttt
     3721 tgaataagct ggcatatgga tatgacctgc cataattgaa ggtaatccag cttctattaa
     3781 gcctgagtat acttttccaa aagaattatc ccattcttcc gtacctaaac tattaatact
     3841 aaaagataga tgctgatctc gttcgtcaat accgtcacca gggaaatgtt tagctgcagg
     3901 caaaattcca ctctcctgaa tacctttcat ataagctaaa ctaagttcta aaactttatc
     3961 agggttttgt ccaaaagatc gactagaaat gataggattt ctccaatttc tattaatatc
     4021 tacaatagga gcaaaactcc agttacatcc aatagccgat gcttcaatcc cagaaactct
     4081 ccctaattca taagcatatt tcgcatcatt tgttgcacca atttttactt ctacaccaac
     4141 ttcagtacca tctgtacaag ctccgttacc acctgcttcc gtattagcag caattaatag
     4201 aggaatttta ctttctgttt gtaagatata gttttgatca tatacctcat cagcagttcc
     4261 tggattatat cgtacagctc caatatggta ctgattgacc atattactca agtaattttt
     4321 atcacgacta gatcccatat taacaaacaa ttgacctatt ttttcttcca aagtcataga
     4381 atcaatcgtc tcttcaatcc aagaaatagc atcttcatct aaattatatg gtttttttcg
     4441 taaatctact tttatagtca ttttctttct cctctactta ttagtaagat ttttccaaaa
     4501 agtatctata tatttttgtt caatatctac ataatctccc gataaacatt taaataaaac
     4561 atcagttctt aagttttccc atgattcttt ttctttattg accaaaatag gataaaagaa
     4621 aactttattt ttatttgcag cttctagatc accaggagaa tcaccaatca ttaatatctt
     4681 atccaaatca caaccatttt caattacact tcgaataata tcttcttttt ttcctttatc
     4741 ttgacaacac agttcatcaa ctaatgataa taattcatgt ctttcccatt cttcttgaac
     4801 agcttcctta ttagctgagc ttacaataat tatcttccca tattttttca gagcttttat
     4861 agtatctcta gcacttaaaa agggtttatc taatcctttt gcttgactaa tctgttgatt
     4921 gacttcttcc gaccatttta aagcttttaa taaatcctct gactgagaat attctatttc
     4981 cctcttcaaa gatggattag acaattcttt tgttagagat acccagtgct ttaatcgatt
     5041 aatcgatgtt acatcatttt tttctaatgt tttaactaaa cctaaaaatc tgtttattcc
     5101 acgagtctct gaatataaat taatattatt ccaatcctgt aggaattttt ctctatgatg
     5161 aacatcaaaa atctctgcag caattggtcc gaaaaagaga ttgtgtttat aagtcatcgt
     5221 atccataaca cagccatctg aatcaataca aaatatccaa ttattcatat ttctctccta
     5281 tttatgtttt tgttttaaaa gctcgcaatt ttctaagact ttatctttag ataatggata
     5341 aaggaaaaat aaaattaatc ctacaatcag aaaacaaatt gatggaatta atgttgaagc
     5401 gttatataac ttttctaaaa catctcccgt ttgtacattt gctaaagagt catagcctat
     5461 aatagaaaga gtccaacctc cgacgcctcc agctagagct tgacctattt ttctagcaaa
     5521 agaatatacc gcatatacgg tcccatcttc tctttgatca gactttactt cttgatcatc
     5581 aataacatca gtaatatttg cccaaataac agtattaaac acacccaaac ccatatagcc
     5641 aaccgaacta agaattaaga atacatacat attactagct tttatcaaaa ataagatcaa
     5701 atatactatt gctgaaaaga acattcctac tgatgccagt tctttttttc catatttttt
     5761 acttaaaaac aaagttaaag gcgaaacaac tattaaaacc aaaataggat tgataaaatt
     5821 cattaaaact attccctgtg catcattata gtaatcagga aatacataat tattcatact
     5881 cataattaat aattgagcaa ggagtaagaa aatagccgca ccaattatag aaagtaatgc
     5941 cctattttta actaaccctt ttaaagattt taacaatgaa actttgtatt gagattggtc
     6001 aattcttact cgttcagtag ttagtttata acatattaaa taacaaataa gtgcacatat
     6061 cgaaaatatt cccgcaacaa ttgtaaaagt tgatccacca cgaactattt gagcgcctga
     6121 atcatctctt gtataaataa ataatggtgt taatacacca atgaccaaac ttgctaaagt
     6181 agctccaaca tttcggaatg tcgatagcat tgttctttct tctgtaatag aagtaatagc
     6241 tgatgccatt gatccataag gaatatttat agatgtgtaa aaaatactcc cccataatat
     6301 atacgtaaca tacatgtata ctaccttaac gctcatagaa gctgaaatca tagaagtttg
     6361 atacattaaa aaacttgcta aagcgactgg accactcatt cttaaaatcc acggtttaaa
     6421 cttaccctgc ttagttgctt tcgatctatc aacaatacgc cccatagtta catcagtaac
     6481 tgcatcgata aatcttgcta ctaaaaataa tgttccaacg gcagctccgc ttatccccaa
     6541 tacttttgta taaaagatca ttaaaaaaga actagcaaaa atgaaagtaa aatcgtttcc
     6601 aaaatctcca aacatatatc ctattttgtc tcttatacca aattttcttt ccatacaatt
     6661 ctctcccttc tgttgttaag cgttttcatt ctgatgaaag tacgtactat actctgaagc
     6721 gtaaagagtc agtatttatt ttttacattt cagagtatta tttgtttttt ctcctaataa
     6781 ttctttatct cataaaactt atctatatat ttactagcta ctcgacttac ttcttcaaaa
     6841 tcaccttttt caacagaagc caataaattc ccacctacac caactgcaac aactcctact
     6901 tcaaaccaat cttctaagtt ctctatactt actccgccag taggcattat atttacgtgt
     6961 ggtaatggag cttgataggc ttttacaatg gatggaccaa aagcagaacc tgggaatagt
     7021 ttaatgatat ctacgccagc agtaagtgcc tcctttatct cagtaattgt catacaacct
     7081 ggtaaataag gaatttgata taaattacaa atctcagctg tttgtcgatc aaaagatgga
     7141 ctaacgatat attctgcccc agccataatt gcgatacgag cagtaaacgc atctaaaacc
     7201 gtaccggctc cgattattac atctttttga tcagcatatt ttttagcaat ttctttgatt
     7261 atttcttcag cattaggaac tgtaaaagtt agttcaatcc ctttaattcc acccgcaata
     7321 attgaattta ttgctttaat ggcctcatcc ttatttttcc cacgtacaac cgcaatgact
     7381 ccagccgctt ctaacttact taaaatttct acttttttca tatctatctc cttcctttat
     7441 ggatacatac tttgtctagc tgtactaact tacaaagaat aaatttaagc ttttatttca
     7501 agtattattt tctacttccg atttcaaaaa aattaaaata attatacgca ttacgataac
     7561 aaatattctc cactatctga ctaagataca tttcatcatc tggaattcgg cctgctttaa
     7621 cccactcgct tagtaattga cataatactc gacgaaaata ctcatggcga ggatagctta
     7681 gaaaacttcg agaatcagtc aacatacctg taaaattcga caacaaactt tgttgtgcca
     7741 tcgtagtcaa ctgttgacgc attccttcag ctgtatcatt gaaccaccaa gcacatccca
     7801 attggatttt ttgaatcata cccccttgga agctttgcat tgctgttgct agtggaagcc
     7861 aatcagttgg atttaatgaa tataaaattg tttttggtaa gcacccactc ttttcagcgg
     7921 tatcgaacaa tgctacaaca ttttgtgtca aactagcttg atcccccatc gaatcaccac
     7981 cgacatctac tccaatttgt tcctttaata tacttgaatt atttcttaaa gcatttaaat
     8041 gcatttgcat cgtccaacca tatcttgtat actcagacat caaatgtaat tgaatacctg
     8101 taacaaattg tgcaatttct gataaatgga aatccgtttt tcctgctaaa gcttttatta
     8161 acagattgtc gagtattttt tcatctgcta tttcataaaa ataagtattc aaaccgtgat
     8221 ctgctaatcg acagccaatt tgatgaaaat gatccactcg ttggctcaat gcttgcttca
     8281 acccattaaa gtctttgatt ggtatagccg ctgtttgact aagttttaaa atatagttcg
     8341 aaaagcctga tgatttgata ctccagacct tatctggacg gaaagtaggt aataccttga
     8401 aagaattatc ctgtttagaa agctcagtat gataccacaa gtcatctgta ggatcatccg
     8461 ttgtacagat ggctttcaca tccatcatct ccaacaattc tttcgggcga taggaatccg
     8521 tttgaatttt ttcgttagca cgtcgccaaa tttctggtgc attcttctca gaaattgtca
     8581 aatcaatatc aaagtaacgt tgtaattcca aatgagacca ttcaaaaata ggattacctg
     8641 gcgccttctc cactgttttt acaaaagcta aaaatttttc ataatcatca cctggaccac
     8701 tgatcaactc ttcttctata ccattagctc gcatcaatcg ccatttataa tgatctccag
     8761 ctcctttatc atttaaccat acctgagtca aattcttgta ttgcttattt tctagcaaat
     8821 atgctgggtc taaatgacaa tgatagtcga taattggttg gtgttttgca aatttatgaa
     8881 aaagtctttt agctatttca ttctctaata aaaagttctt atctaaaaac atatttaaac
     8941 actcctttcc ttccgttagt ttttagaaga gaaaattttt cctctaaata aactccctta
     9001 tataattatt tactcgcccc cttttcaagc gcttcccaaa taccatttaa ataaactgca
     9061 cctagagctc ggtcatataa tccataacca ggtctaccgg tttctcccca aatcatccga
     9121 ccatgatctg gacgaatata gccattaaat ccattatcgt acattgtttt taatatctta
     9181 tacatatcta gagaaccata ttctgattta tgcggcgctt cgtaaaaatc gagtccattg
     9241 tcacttaaat atttgatatt acgaacatgt gcaaaaggaa tccgatcccg ttgacaaaat
     9301 tcagatagaa tttcatagac atcattatct ggtcgttctg caatacttcc agtacataat
     9361 gtaataccat tagaaggaga atccactaca ttacacaacc aatctaaatc gtcacgattt
     9421 ttaacaatac gaggtaatcc gaaaatggaa tatggtggat catctgggtg aactgccatt
     9481 ttaatcccaa cttcttcaca tgccgggata attgctttga gaaaatatgc aaagttctct
     9541 cgtaatttct cttcatcaac ttctttatag gcttctaata attgcttaat ttgtgataac
     9601 cgatgaggtt cccaacccgg caattcaaaa ttattagtag aattttctac ttctctaata
     9661 atttcctccg gatcagtagg gatatttttt gaaatatagg ctaacgtaga agacccatca
     9721 tctaaacgat aatccaaatc cgattttacc caatcaaata ctggcataaa attataacag
     9781 ataacctcaa tcccaaattc tgccaaattg cgaatcgtct gtttataatt ctcaatgtat
     9841 tgatctcgtg ttggtaaacc aattttgata tcatcatgaa cattaacact ctcaataact
     9901 ttcaaggtaa gtccattggc ttcgacttca tcttttaaag atttaattcg ttctctcggc
     9961 caaacctctc cggctggtat atccattaat gttccaacaa ttcctttcat tgctgggatc
    10021 tgacgaattt cttgtagtga gattttttct tcatttgccc cataccatct aaaagtcatt
    10081 tccatagtta ctttcctcca acttctgtta tatatttatt gattgtttta cgaactgctt
    10141 gaggacctac tagcatttcg caaaagattt tttcagtttt ctttcctacc ccaacttgat
    10201 ataaatctaa accaaagatt tctttatttt ctaaaatttc ctgtaccgca caatgaatct
    10261 ttgaaggatc ttgttcacct aatttcaata ctgataattt atcctgcaac tctaccaata
    10321 acggatcagg acttggcaca aaattttccc cttgatcatc gactgccagt aaataacgta
    10381 accaagcagc aatagcgata ggaataaatg ttagattttc tgcatttccg tctttttcga
    10441 catacttttt aattgtttca ccatagcgaa tcgctacttt ttgagaagta tctgttgcaa
    10501 ttcgttgtgg tgtatctgga atatagggat taggcaaacg tttattaatt acttcatcaa
    10561 taaattgttt tggatcaata atgcctggat tttcaactac tggtaaacct tctaaataac
    10621 ctattccttt gattaaacca accaaatcat catcctgcat ctctgctgaa attgattgat
    10681 atcctagcag acaacctgtt actgccaaag cagtatgaag tggattcaag cttgtcgtaa
    10741 ccttcatgat gtctgcttta tcaaccgtct ctctatctgt cataatcaca ccagcctttt
    10801 ccaatttagg ccggccattt ggaaaactat cttcaataac taaataatga actacctcag
    10861 tattagtgaa agcagcaata ttggtatgtt tttttgtgtg aatgatatct aaatcctcga
    10921 gccccatcat caatagatct tcctttacat tctctgaagg atttggcgta attcgatcaa
    10981 tcatactcca aggaaaagtc actttttctt tatcctttaa ataagcaata aatttttcag
    11041 aaacatatcc ttctttttgc caagcttctg caatcgtaaa aatactatct tgaaaacgtt
    11101 gaccattctg tgagaaatta tcagtagaaa ccatcgcaat cggcaactct ccagcattaa
    11161 atctgctata taacaaggat gttacaatac tcatcgtatg ttgaggattg ctaggtccag
    11221 atttgatatc agctttgact accgaaaaga attgcccttt agaatctttt aaactgtaac
    11281 ctttttcagt aatagtaaac gttgtaaact gtaatttagg atcttcaaaa tatttaacca
    11341 ctctttgata gtccgctata ttcttcggat tgcaatataa actatcagcc gttactgcca
    11401 aaagtctttt tttgagttca ccatctccac tcataacaat ttccaaaatg tcattgttat
    11461 agtcatgata agctttttcc actacttctt catcaaatgt ttcacacaca actactcctg
    11521 tttctaattc attttgatct gctaagcatt gagcaatttc tgcatgaaat cctcgataaa
    11581 gatttcctcc accaaaatga agccatactg gatgttgctg gccttcttgc ttaacttctt
    11641 tgacatcaaa acacggcagt agaatctgca tttcattgaa ttcttcgata gaattttgcc
    11701 aatttttaat cgaaaccata attaacctcc tcaaactgat atgttagttt tgatacattt
    11761 tatattttaa aacccattgt aaccgttgtc aatataaaaa gggaataaac ttgctttttt
    11821 ctaccatatc agaatgattg tattttttat tcagaaaaat aaaatactcc ttctttttgg
    11881 actgatttta tcgaaactta ttttttcttt ttttcatgta ttcttttatt tttataacta
    11941 gtgtattact tcaaaatttg atattatatg gcatgaacag aattaaaata tcagttaaaa
    12001 taaaagttaa ggagcattgt gtatgacaat taaaactagt ctccaaaatc aagcttacca
    12061 atctattcgc cgacaaatta tctatgctga cttagaacct ggaaaaaaag tatcagaaaa
    12121 aaaattagaa gaaacacttt ctattggtcg tacaccaata cgtgaagcac tgattcaact
    12181 ccgacaacaa ggtcttgttg atacaattcc tcaaagcgga aattatatct cattaattga
    12241 tttagattcg gccaaaaatg ctcgatttgt cagagaacat ttggaacgac aaattatgtt
    12301 agagtgttgt gccaaaatga ataaccaaaa aaaacggatt ttagaaacta taatttcaga
    12361 acaagaaaaa gctgttagtc aaaaaaatgc tcgtagtttt tttcataatg ataatttatt
    12421 ccataaagct tgttttgaaa ttgcagagcg agaagaagta tggcaatggt tagaaaataa
    12481 caatacacac ttagaacgat ttcgatggct acgagtaaaa acggaaggat taaagtggga
    12541 aaaaattatg gaacaacatt atcaactatt tgaggcatta acaaaacaag atccagaaga
    12601 agctaacttt ctaacatcgt tgcatcttca tttaatgctt aatgagcaaa atattgtttt
    12661 agctactcat ccaaaatatt tcaaaaatat ataactttta actcttttta aattaaatgg
    12721 ttgttctgat cagaaaaata tcgcttgttt tcttattatg taatatattt cataaactat
    12781 tctgcataac aactattgtt tgtacttttt acattggata actaattatg actgctgata
//
LOCUS       DAYYAR010000083        12738 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000083 DAYYAR010000000
VERSION     DAYYAR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.083"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHAT0_001925"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHAT0_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8451857.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACHAT0_001926"
     CDS             complement(323..892)
                     /locus_tag="ACHAT0_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8451858.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACHAT0_001927"
     CDS             1078..1980
                     /locus_tag="ACHAT0_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8451859.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHAT0_001928"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHAT0_001928"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8451860.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHAT0_001929"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHAT0_001929"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8451861.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHAT0_001930"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHAT0_001930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8451862.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACHAT0_001931"
     CDS             8548..8733
                     /locus_tag="ACHAT0_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8451863.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACHAT0_001932"
     CDS             complement(8795..9991)
                     /locus_tag="ACHAT0_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8451864.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACHAT0_001933"
     CDS             complement(10161..11597)
                     /locus_tag="ACHAT0_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8451865.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACHAT0_001934"
     CDS             11890..12699
                     /locus_tag="ACHAT0_001934"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8451866.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4012 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgacaatt
//
LOCUS       DAYYAR010000084        12673 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000084 DAYYAR010000000
VERSION     DAYYAR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.084"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..475
                     /locus_tag="ACHAT0_001935"
     CDS             32..475
                     /locus_tag="ACHAT0_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8451867.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(481..1665)
                     /locus_tag="ACHAT0_001936"
     CDS             complement(481..1665)
                     /locus_tag="ACHAT0_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8451868.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1933..2631
                     /locus_tag="ACHAT0_001937"
     CDS             1933..2631
                     /locus_tag="ACHAT0_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8451869.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2638..3315
                     /gene="nth"
                     /locus_tag="ACHAT0_001938"
     CDS             2638..3315
                     /gene="nth"
                     /locus_tag="ACHAT0_001938"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM8451870.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3441..3974)
                     /locus_tag="ACHAT0_001939"
     CDS             complement(3441..3974)
                     /locus_tag="ACHAT0_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451871.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4271..6652)
                     /locus_tag="ACHAT0_001940"
     CDS             complement(4271..6652)
                     /locus_tag="ACHAT0_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8451872.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6642..7289)
                     /gene="recU"
                     /locus_tag="ACHAT0_001941"
     CDS             complement(6642..7289)
                     /gene="recU"
                     /locus_tag="ACHAT0_001941"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8451873.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7353..7892
                     /locus_tag="ACHAT0_001942"
     CDS             7353..7892
                     /locus_tag="ACHAT0_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8451874.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            7982..8401
                     /gene="gpsB"
                     /locus_tag="ACHAT0_001943"
     CDS             7982..8401
                     /gene="gpsB"
                     /locus_tag="ACHAT0_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8451875.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8423..8790
                     /gene="rnpB"
                     /locus_tag="ACHAT0_001944"
     ncRNA           8423..8790
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHAT0_001944"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            8999..10165
                     /locus_tag="ACHAT0_001945"
     CDS             8999..10165
                     /locus_tag="ACHAT0_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8451876.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10211..11707
                     /locus_tag="ACHAT0_001946"
     CDS             10211..11707
                     /locus_tag="ACHAT0_001946"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8451877.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11788..12471
                     /locus_tag="ACHAT0_001947"
     CDS             11788..12471
                     /locus_tag="ACHAT0_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8451878.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12480..>12673
                     /locus_tag="ACHAT0_001948"
     CDS             12480..>12673
                     /locus_tag="ACHAT0_001948"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451879.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4251 a         2223 c         2620 g         3579 t
ORIGIN      
        1 caatataatg aaagaaaaag aaggtgtaga aatggataaa agacaactgc taatagaaga
       61 atatctggat ttagctaatg agattattca tcgtaataaa ccagaaatgg aaaaagaatt
      121 tgcaggattt actctaaatg aactagaagt gattgaacat atcgggaaaa tccctgatcc
      181 taacgtgaca aaattagcga atgctagtta catgactaga ggagccatca gtaagctaac
      241 aaaaaaactg atagctagag ggatcataaa cacttatcaa agcgaagaga ataaaaaaga
      301 aatctatttt caattgacgc caatcggaca aaaaataagg aatagacacg agcaactcca
      361 tcaaaaatgg gtacagcgag atgctactgt atttgaaaat atgtcaaaag aagaatttga
      421 cacagtattt cgttttatcg gtcgatacag agaatttata cgaagtagca aatgaagcta
      481 ctatttttta ttgtagtgat aataggcaaa aatcataagc aaaaatccga ataccgtcgg
      541 aagaaatggt gctgcagcat aaagctgtcc tgcgattagc gggccgatga cgcgcgatag
      601 agattgtata ctttgggtac cgcccattac aagaccttgc tggttatctg agacagaatt
      661 ggatagttgg ccattgaata caggagtaaa aatagaatca ccgaaaccat aacagataat
      721 cccaaataaa aacaaaatcg gcagtagaag aattccagat aatgtgatga atagataggc
      781 gaggatttca ctgaacatcc ctattcttgt gatctgctgt tctgaaaatt ttttgagcaa
      841 tcgaggcata atgaacaatt gcgagacagt atccaagatt ccaatcaaag agaagcttaa
      901 accaaccaac ccagccttcc actgaaaggc gtcaatcgaa aattgagaaa agatagattg
      961 aagagagcca gctgccaacc aaacagcaaa acctgctgtc aataaaggaa taacggatga
     1021 aatcttgaat aattgttgta attggtgaaa aggtcttaca tgggagaaac ttaaatctac
     1081 tgaacgtttt tttattggaa ggctttctag cataaatgtg tagccataaa cggcatttaa
     1141 gaaagtaaag agtgctccaa taaatatagg aacagaattc cccaactcag ctaatagacc
     1201 ccctataata ggacccagcg ttgttcctat accgactaaa gcacccatcc atccaaaaac
     1261 tttggttctt tcattaggtt ccgtgatgtc ggcaaagtaa gcatataagg tggagatttc
     1321 tcctgccgtc aatccttcaa tgatacgacc aaggaacaac atccatatcg aatttcctaa
     1381 accaaaaagc aaatatccga tacttgatcc taataaactg ataataagga tcggatgacg
     1441 cccaaaatga tcacttaagc taccgagaat gggggcagat aagaaggcag ctagagcata
     1501 aacagacatg agcaaagtga tatagaaagc ttgctgatgt atggagtgag aataaggcag
     1561 cgttaaaaac ggaagtacag gactgacgat agtaaatcct aatccagtga ataagacaga
     1621 gacgtatcca aaaactttag aatgtttatt tgtgaaactg tacatgtaaa aactccttta
     1681 tgcaataatg ttaacttgga aacaaaaata gtgtatagta attttgtttc cttgtcaaca
     1741 ttctaaggaa cagcgaaatc tacttaattt atttattccg aaaagtgaag atacgcatca
     1801 aaaacatgaa caaacaaaaa tcaagtagac gacattgtaa atctgaccga tcagtgaagt
     1861 aaatagaaaa gaaacctgtt cttgcttccc atgttttttt tacgtatact gtgattgagc
     1921 ggaggagaaa tcatgttgaa tttagaaaat tacttaagcg ctggacaaac agtcatatct
     1981 aatcttttac ttaaaaatta tcataggatc ggtctacaat ctgaagaatt tttatttgtt
     2041 ctacagttgc atatggcaca gcttgaagga gatactttcc ctgatttgca gatgattgct
     2101 atggatatgg gaatcaaaca agatcagatt tttcaaatac tggatcgtct cgtaacaaat
     2161 ggatttatca agatagaaac gacgattgtc aacggtaaaa aagcagatcg atataatctt
     2221 taccccatct atgatctatt aggtgagtat ttaaaaacac aagaaaaaaa atacgaagaa
     2281 aaaacacaag aaaaagaaat ccagtcagtg tatcaattat ttgaacaaga atttggacgc
     2341 ccgctttctt cgatcgagtt ccagcggatc ggacaatggc tagaagaaga ccattatcag
     2401 ccggaaattt tgaagctagc actgcgggaa gccgtattaa atcaggctta tagttttaat
     2461 tacgttgacc gcattttgct atcgtgggaa agaaaaaatc tacgtacaaa acaacaagtg
     2521 gaagaagacc aaaaacgaag aaaacagcag cttcttcaaa gagaagtaga acagtcagct
     2581 caacaagaag aattgccgaa agtctcactg aaaaattggt tggaggagta gcggaatatg
     2641 ttaagcaaac aaaaaacaat ggaagctcta gaaacgatgt atggaatgtt tcccgaagca
     2701 catggagaac tcaagcacaa taatccattt gagttattga ttgcagtgat cttaagtgcc
     2761 caggcgacag atgtatcagt gaataaagca acgccagatt tgtttgcctc ttttccgaca
     2821 cccgatgcgt tagcagaagc ttcaatcgat gaaatcattc ttaaaataaa aacgattggt
     2881 ttgtatcgaa ataaagcaaa aaacatcaaa gcgtgtgcac agcagctgat tgaacgtttc
     2941 gatgggcaag tgccaacgtc aagagaagaa ttgatgtctc ttcctggtgt aggcagaaag
     3001 acagcaaatg tcgtattggg ggatgcattc gggataccag caatcgcagt agatacccac
     3061 gtggaacgtg tctcaaaacg cctgaggatc tgcaaactag atgctacggt catggaagta
     3121 gaagaaacac tgatgcggaa agtaccacaa gaattatggg taaagacaca tcatacactg
     3181 attttttttg gtagatatca ttgcacggca agaaatccca agtgcgaggt atgtcctttg
     3241 ctatcaatat gtcaagatgg gaaaaatcgg atgcgtctaa aagaaaaggc gctaaaaaag
     3301 aaaccacgcc tgtagacgtg gtttctttaa ttgcaaataa atgtatgaat tatcttgtta
     3361 ttatggagaa aagctgtttc gtttatcttt tttgcatgaa aaagtaaatg tacatactca
     3421 gtagctagga tggtaactta ttacaattta ttgcgataaa aataaaagtt gttattttta
     3481 ttgggaaaat acccgatgta gaaaataatg ccttccaata tacctataaa ataagtccag
     3541 tattccaaag gattatataa ataagtgacg tatctatcta aaggtgtaat aaaaatgata
     3601 agtgataata aaaaaatacc atttataact aaacataaac taatggattt gggcggtttc
     3661 aggctcatca gaaaaatata gattctggtg aacaacaacc ataaaaaagt gagaatcatt
     3721 gcatgagagc ttctttccat aaattttcct ttaagagtag tgcttaaaag tacagaaaaa
     3781 ataataaatg caaggatagg agcaattaaa gtaaatttat ttaataataa acaaaatttt
     3841 tcagcttttt tattcttgga ataacgaggt attgttttcg ataaaaaaag atggtgcaga
     3901 caaattatga taattaagca tactatcaga taactcaaaa tgaagccttc tttcaaaaaa
     3961 ttattcaaaa ccatatattg tattttttat ttgataataa caatgggatt attaagaagt
     4021 ggcactaaat aaaagactag tgtgtattta gccgatttaa ttataaacaa gtaaatggat
     4081 aaagtatatg taatgataaa agaaaaatgg attttctaat ttttaagcta aatgttagat
     4141 cacacatgta tatgctggat agtcttataa aaaatcatgt aatacagctt ggtacatgat
     4201 ttttatttgt agctgttttt tacatagaca caaaaaagaa accacgcttg tagacgtggt
     4261 ttctttttta ctatctgttt ccagaagctg aggtgctgct ggatggtgta tggtcatttg
     4321 cagcatccgc tccgctagtt gctggctcac tggaagaagc aggaggagca gatgaagacg
     4381 gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt tgaagacgct ggctcgcttg
     4441 tttctgctgt cgtgctttct tttgaggact cagcagttga ttcgctttga ctagtagagc
     4501 tagagctttg agtcgttgtc tctgttgttg agcttccagg agtctggaca tagctggaag
     4561 atggaatagt tgttgatggt gtaattgcat tcgatctagc tgtatattga tctttgtagt
     4621 acagttcacc gcccacacga attaatccgc ttggcatctc ccagtctgta tttgtcacgt
     4681 tagctgaaac atactgcata agctcgcgat aaacatcaga ggctacgtgt gacgattctg
     4741 aagtgacagg cgtcattttt ttattgtaac ctgtccacac ggaaatcgaa taatttggcg
     4801 tatagcccgc aaataggata tctggataca ccccagaaga aataccaagt tttgcatact
     4861 cgtcatctgt gtaattggat gttccggtct taccggcttg atataaccca gcgatttgcg
     4921 cattcgtacc agttccttca gtgatcgtat cttttaaaat gtctgtgatc atataagctg
     4981 tttctggaga catggctgtt tttccatctg gctcataagt ttcttctgtt ccgtcttgga
     5041 agacgatttt attaacatat tgaggtttgt aataagtccc tccattggcg aaagccgcat
     5101 aggctgctgc catttttaag gaagaagctc cgtattttgt cccatcttgt tcttccgtat
     5161 tactagagat cgcattcgat tgatggatcg tactgtattc gatcccaaga ttttttaaga
     5221 aagaagccac tttatctgaa ccgacttcat tgaataattt gactgctgga acattacggg
     5281 ataagtaaag tgcttggcgt aaagtaatgg tgcccatata ttgattatcc cagtttccta
     5341 caggagtcga agtcccttca tagttatacg gtgcgtcagt aatagttttt ccagtagaat
     5401 atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg tttcatcgta gaaccaaaat
     5461 cacgggaagt attcactgct aagttgttgc ctaaagttac gtcttctgcg atgtgacgtc
     5521 cgccaatctg ggcttttact ttcccggtat tggtgtcgat aagggtggag gcaacttgca
     5581 tctcgtcgtc tgggtaagag acatactgat ccgtgttgac gatatcatat agtttctttt
     5641 gggcgtccag atccaaattc gtataaatct ctaaaccgtc tgtgtagaca ttcttatctg
     5701 ttttttcttg tacttcattg atgacttctt tgacataatt atcgacgatc ttagtattgt
     5761 catcggattg agttagttct tgcagaccat ccgttaccgg tacattaaca gcttgatcat
     5821 attctgtttg agagattttt tcgttttgaa gcatcgtata caaaacagta tctcgtcttt
     5881 tcttcgcctg atctggataa acataaggat cataggcgga aggggcttga ggcatcccag
     5941 caagcaatgc tgtttgcggt aaagaaagtt ctgataattt cttaccaaag tacatctcag
     6001 aagctgtctc catcccgtac aagccgtttg acatatacac tttatttacg tagtaggtca
     6061 agatctcttg tttcgatttt ttttgttcca atcttacagc catccacgct tcttgggcct
     6121 tgcgtttcag agtctgatcc tcggcactgg tggagaagaa ggaaagtttg atcaattgct
     6181 gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa gttagagagg gcagaaccga
     6241 tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg gcgatcttct accgacacga
     6301 tcgcatcttc taatgttttc ggtaattcat tcgcagaaat tttttcacgc ttttctgcgc
     6361 ctaaatcttc gaatagttcg ccatcttgtg tatatagttt ggaagatacg gtagcgtcta
     6421 attttttatc cgtcaattcg ggcgcatctt ttgcgtaata ccagaaaagt cctacacctg
     6481 ctaaaaagag gatacagaaa aatgagagta ttcctaaaag gatcttgata aataatcctt
     6541 ttttatgtgt tccagaggac tttccagaac ctttgccaga attcttcttc acactctttt
     6601 taggagtagg ctgtttctgt cgtctagagc ttctagtttg ttcatttgcc attcttgctt
     6661 tctccttcaa tagataatgt tgataaatag aagtcaaccg cgtccaaata aggaatccgc
     6721 ggagaaatac cagttttgat ctctattcct agttcttcga tcaaagataa aggtagtgat
     6781 ttgcgtcctt tttccttttg ggcgtcccag taagcaatca aatgaacact attcaaataa
     6841 aaacagcgct tcaaagacga aaaccataac aagacaaagc agaccccatt ttgttcgagg
     6901 catgctttca tatgttcgat ttgatgggca tggaagtttt taaagggaaa agatgttttg
     6961 ttcttggttt cttttgcttc aaagtccaag taaaagcctc gatagacgcc attatagtct
     7021 gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag cagcacttct cttaggatag
     7081 tctactttca cgatttggat aggggtaggc tttttatgga taaccgccaa cccgcgggaa
     7141 agataatatt tattactttc attgattgct tcttcaaagc gcatcccccg attgccgaat
     7201 tcagtgatct gatttctttt ttcttgaatt ttttcgacgg gagaggcatt cttataataa
     7261 ggttggccat ttggatagcg caataccatc tttaatcaca ctcctaggcc acattatagc
     7321 aaaaaagcgg atagaaagaa aaggaatctg agatggataa gtcaaaagta atctatgtgt
     7381 ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa tgatcctaaa atccaagttg
     7441 ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga agaagggctg gaatgggtac
     7501 ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca aatagtagcg gaattgaaag
     7561 aagaatatcc ggaactatct cttggtattt tgtatccttt ttctgatttt ggtaataact
     7621 ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca attagcagat tatgtcgaag
     7681 ctgttagcca tcaaccctac caatcaccta gtcagctaaa gaatcacact cgttttatac
     7741 ttgagcatac agtgggctgt ctgctgatct atgatgaaga attccctgga aaaacgaagt
     7801 atttttttga agatgcacag aattaccaag cagatcatcc ctacgaaatt cggctgatca
     7861 gtatggacga tcttcaaaat tttagtgaat aatcgatatt gtgtttgatt tatcaacttt
     7921 tttcctatac aatgaaaaag gtagaaatgt tattacgtaa atgaaaacta gaggtgtaaa
     7981 tatggcgaat ttggtttata gtcctaaaga cattttacaa caagaattca aaacaaaaat
     8041 gcgcggctat gatcccgttg aagttgatga attcttagac aatatcatca aagattatga
     8101 aacttacagc aaagaactcc ttgctttaca agaagaaaac gatcgattga gtgcaaaagt
     8161 agctcaatta tctaaaactc aaggagcagc tcaaacacgc gtgcaacaaa cagaggcacc
     8221 aaagagcgca gcagtgacca attttgatat cttaaaacgc ttgtcaaatc ttgaacgtga
     8281 agtatttggt aaaaagcttg atcaacaagc aagtgcagtc aaaccagcac aacctaatcc
     8341 aaataactac acaaatgcag acacaagctt agatgataat gaaaaaacac gtcaatttta
     8401 atttttgatt aatgaatttg cggttttcgg gtaatcgcgg tctgacttga tcaggctgag
     8461 gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt cgtgcttagc gaaaccataa
     8521 gctaaggtac tctttggagt aacggcagga aaacaagcta aggcttcggc tatgcttaag
     8581 tatccttgaa agtgccacag tgacgaagcg gctggggaaa cccatccggt ggaacgcggt
     8641 aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc tttttgtctg gaagaagaac
     8701 aggacaaaaa ggcagtataa ctgcagatag atggttaccg ctttgttttt tctccctgga
     8761 aaagcaaagt acagaacatg gcttatagaa aaccgcaaac atcagggaat taggtcgtga
     8821 caaaagtaat gcgttataag aaacaagaaa aacatttgaa agctattttt caatacttca
     8881 gatgaatcgt tcttattaca aaacaaactt ttgtatcacc ggttattcgg aatttgagta
     8941 aaagtgtggg gtaggactga tgtcccacct ccttttttta taaataagga gattgtgtat
     9001 ggaaaaaaac aggaagttca acctcgtcgc aactgcggca agcgggctag aagcactggt
     9061 tggtaaagaa ttaagagatc ttggaatcga atgccaagtt gagaacggac gtgcacgttt
     9121 tgaaggaaca atggaaacga tcgctacagc taatttatgg ttacgtacag cagatcgagt
     9181 aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat gaattgtttg aaaacgtaaa
     9241 agcattgcct tgggaagatt ttcttccact agatgcagct tttcctgtag ctggaaaatc
     9301 gatcaagtcg aaactatata gcacacctga ttgccaagcg atcacaaaaa aagcaatcgt
     9361 tgaacgtctc cgtacctatt atcaccgtcc agcttctgta ccactgacgg aaactggcgc
     9421 acattttcag ttggaagtag ctttgctgaa agaccatgtg atggtaactc tggatacgac
     9481 aggaccaagt ttattcaaac gtggttatcg cttagaaaaa ggtggagcac cattgaaaga
     9541 aaacatggca gcagcactta ttatgttgac aaattggcga aaagaccgtc cattttatga
     9601 tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc ttaataggcc ataatatcgc
     9661 accaggattc aatcgtgagt ttgcttgcga atcttgggat tggttcagtc aagaaatcat
     9721 ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat gatattgaat tagatattac
     9781 tggttcagat atcaatggac ggatgattga aattgccaaa gccaatgcag aagaaatcgg
     9841 attaggctct tcgattacct ttaagcaaca agcagtcaaa gacttcaaaa cggataaaga
     9901 atacggggtc atcgtggcta atccgcctta cggagaacgt ctaggagaag aagaaactgt
     9961 tcggaaatta tataaagaaa tgggcgatgt attccgtcca ttaaaaacat ggagtaaata
    10021 tatcttgact agcgacttag cgtttgagga attttacgga caaaaagcaa ccaaaaaaag
    10081 aaaattgtac aatggtgcat tgcgtacgga tctattccag tattggggaa ctcgtccgcc
    10141 tagaaaaaaa gagaaagacg aataatctta ctaattgatt cattcaattg gatgtttgga
    10201 ggaataggga atgaaagaaa aagagttttt acaagaagta aaagaaatca atcttctaca
    10261 acaagcttta ggaatcctcg attgggatac acaaacaggt atgcctgaaa aagctagtgc
    10321 ataccgaagt gaagtagaca gctatcttta cagcatttat ttttcaaaaa aaatcggtcc
    10381 aaaaatcaaa gaagccattc gctatttttc tgaacatccg gaagaattgt cggaagttgg
    10441 aactgccgtt tttgccaaag taaaagaaga gtatgatttg gaacatcgcg taccggaaga
    10501 tcttatgaga aaatatacag ctgccgtttc ttcagcacac ggacaatggc aaaaagctag
    10561 agaaacacaa caattttctg attttcaaca cgcattgaat caaaatattg aattaacaaa
    10621 acagttgata ccttactgga gaaaagatga aaagacgaat tatgatgttt tgctgaatca
    10681 atacgaaccc gggatgactg tcgaaatcct tgataaggta tttgatcagc ttaaagaagg
    10741 aattctttcg atccgccgta cgctccaaga aaaagggact gaacctgaaa cagacttttt
    10801 aaatcggaga atgacgaaaa aacaacagaa gagatttgta gtgaaagtaa tcgaacaact
    10861 tggttatgat tttagtcgtg gtcgtttaga tgacactgtc cacccattta tgactggaat
    10921 taatccgaat gatgcacgaa tcacgacacg atggaacgaa acagatttca aaatggctgt
    10981 ttttggagtg atccacgagg ctggtcatgg tatgtatgaa caagacatcg atgataaata
    11041 tgcgtataca ccggtttatg aaggtacatc aatgggtatc catgaatcac aatcgttgtt
    11101 taacgagatc attattggaa gtagtcgcag cttctggaaa aagcaatatc ctttctttca
    11161 agaatgtgca gaaggaacat tcgatgatat tccttttgac aaattttatc gttcattgaa
    11221 ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt ttgacttacc ctctccatat
    11281 catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa ggggtagatg ttgctgaact
    11341 acctgaaata tggaatcaaa aatatgaaga gtatctggga atacgaccaa caaatgatgt
    11401 ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc tttggttatt ttccctctta
    11461 tgcattaggg tacatgtatg cggcacaatt acttcattct ttgcagcaag aaatagatgt
    11521 ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa aactggatgt cagagcatat
    11581 ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc atggatgcaa cacatgaacc
    11641 gcttaatccg caatacctga tcgactatat gaaagacctg tattttaatg tatatcaggt
    11701 aaaataaaat acaatttaca aagatagctg aaattcatct aatctataga tgttttcagc
    11761 tatttactta agaaagaagt ggaacgaatg gataaattac aagtgatcgc tgattatagt
    11821 atggaccaat tagcgcatga tcaaacagga catgggagcg atcataccaa aagagttgtc
    11881 aagttagctg aaaaaatttt ggagacggaa ccgcaggcag accgctttgt gacgcttgca
    11941 gcagcttacc tacatgatac gattgatgat aaagtcgtta aaaatgagaa cgaggcaaag
    12001 caacagcttc gtgtattcct tcgctctttg ccaatagtag aggaacagat ctcgatgatt
    12061 ttttccatta tcgaaaatat gtccttttct aaaaatctct ctgaagcggc cgaactatct
    12121 ctggaaggta agatcgttca agacgctgat cgtatcgaag cattaggagc aatcggcatt
    12181 ttgcgtactg cttatttcgg cggtggacat gggcacccga tttttgatcc tgaactgtat
    12241 ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca cgacagtgat caatcatttt
    12301 tatgaaaaac tgttcttgct gtctgataaa atgaatactg attacggata tgaagaagcc
    12361 aaacgaagag aaaccttcat gcgtgaattc ttagaagaat tttttctgga atggtctatt
    12421 gacgatgaaa attttactcc agacaattgg atcaaaaata acaaaaaatg atggaggttg
    12481 tgacagaagt ggtcagctcc aagatatttc ggatcatttt ccgtaaaagt tacttctgga
    12541 acactgttta ccaagaggtc gtgagcctgc cgttcagctc cgagtcacaa ggaacaattt
    12601 caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg tgattcggaa ttggcaggag
    12661 aacaccgttt att
//
LOCUS       DAYYAR010000085        12654 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000085 DAYYAR010000000
VERSION     DAYYAR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.085"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(277..639)
                     /locus_tag="ACHAT0_001949"
     CDS             complement(277..639)
                     /locus_tag="ACHAT0_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451880.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(713..1093)
                     /locus_tag="ACHAT0_001950"
     CDS             complement(713..1093)
                     /locus_tag="ACHAT0_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8451881.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1175..2350
                     /locus_tag="ACHAT0_001951"
     CDS             1175..2350
                     /locus_tag="ACHAT0_001951"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8451882.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2427..3011)
                     /locus_tag="ACHAT0_001952"
     CDS             complement(2427..3011)
                     /locus_tag="ACHAT0_001952"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8451883.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3212..4210
                     /locus_tag="ACHAT0_001953"
     CDS             3212..4210
                     /locus_tag="ACHAT0_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8451884.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4322..4831
                     /locus_tag="ACHAT0_001954"
     CDS             4322..4831
                     /locus_tag="ACHAT0_001954"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8451885.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4861..5520)
                     /locus_tag="ACHAT0_001955"
     CDS             complement(4861..5520)
                     /locus_tag="ACHAT0_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8451886.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5522..6286)
                     /locus_tag="ACHAT0_001956"
     CDS             complement(5522..6286)
                     /locus_tag="ACHAT0_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8451887.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6294..6971)
                     /locus_tag="ACHAT0_001957"
     CDS             complement(6294..6971)
                     /locus_tag="ACHAT0_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8451888.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7008..8393)
                     /locus_tag="ACHAT0_001958"
     CDS             complement(7008..8393)
                     /locus_tag="ACHAT0_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8451889.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8603..8687)
                     /gene="ffs"
                     /locus_tag="ACHAT0_001959"
     ncRNA           complement(8603..8687)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHAT0_001959"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="ACHAT0_001960"
     CDS             complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="ACHAT0_001960"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8451890.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9332..10189
                     /locus_tag="ACHAT0_001961"
     CDS             9332..10189
                     /locus_tag="ACHAT0_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451891.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10270..10605)
                     /locus_tag="ACHAT0_001962"
     CDS             complement(10270..10605)
                     /locus_tag="ACHAT0_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451892.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10672..12042)
                     /locus_tag="ACHAT0_001963"
     CDS             complement(10672..12042)
                     /locus_tag="ACHAT0_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8451893.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12120..12653)
                     /locus_tag="ACHAT0_001964"
     CDS             complement(12120..12653)
                     /locus_tag="ACHAT0_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8451894.1"
                     /translation="MGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYPI
                     YTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAKS
                     DLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWDK
                     NEVTDEYLENILKEQSP"
BASE COUNT         3763 a         2566 c         2117 g         4208 t
ORIGIN      
        1 tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt tatcatgtat
       61 ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt cgatcaatta
      121 ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca ctaaaaaact
      181 atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa gaagtttgaa
      241 aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca ctccctctac
      301 ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat atccttcgcc
      361 taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa cattttcctc
      421 tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga tatgaactat
      481 ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa gttccgctaa
      541 cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac catcctccaa
      601 atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct cgatcaactt
      661 gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact ttttatttct
      721 tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt aactgctccg
      781 caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt ctgcgatatg
      841 gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt ttggaatatt
      901 catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat gaatggatat
      961 ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc tcgttatcaa
     1021 gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt atttgtccaa
     1081 ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa atagctcaaa
     1141 aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata aacagatttc
     1201 aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc aaatggatga
     1261 cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg tagctaaagc
     1321 gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg ataaactata
     1381 tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga aagaaaatat
     1441 cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt ttacaaaacc
     1501 aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta ttatagaaac
     1561 aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt tcatcatgga
     1621 cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga ttttatgcaa
     1681 tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt taagcgaatt
     1741 gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt taactttatt
     1801 cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc tactagttgc
     1861 ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa tggtatttat
     1921 caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc aacagcacga
     1981 aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg gagaatggtt
     2041 aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt tttttgaaca
     2101 gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt ggttagattt
     2161 taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga aaggaaaagt
     2221 cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc gcttgaatat
     2281 tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg ctttagagac
     2341 aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt tcgtcctttt
     2401 tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc aatcactaca
     2461 tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc tactgtgtcc
     2521 atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg agttccgcca
     2581 cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg tccaagcata
     2641 ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt gtttggacct
     2701 gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa ttcatcttca
     2761 aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc gccgccttgg
     2821 atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc ttttttggaa
     2881 agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg aacagtaatg
     2941 tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa atctagttgt
     3001 gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa taacataata
     3061 cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac tagatacatt
     3121 ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc tcaattaaat
     3181 tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta caatcatcgg
     3241 tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag ctaaaacaaa
     3301 aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc ccgaaaaata
     3361 tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg atcaattaga
     3421 aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc tctcgcttac
     3481 tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt ccaaagcagt
     3541 cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag ataatgctga
     3601 atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt atgctggaaa
     3661 aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga tgttagagcc
     3721 tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc acgaacatag
     3781 tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca tcgatggatt
     3841 atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa gtgacgagac
     3901 gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta atttagaaca
     3961 tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat cagatatgtc
     4021 tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag gaaaagtgaa
     4081 actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt tacactatat
     4141 taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata aaaatcttcg
     4201 tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc agtttcttgc
     4261 atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg ggagcaataa
     4321 tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga aggaacgtgg
     4381 cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact atatttctga
     4441 tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc gtgaagtaca
     4501 taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa aattgatgag
     4561 tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg agatcctagc
     4621 cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt atatcgacaa
     4681 ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg tccatacctt
     4741 cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag cacacaaaga
     4801 accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga aaacgtctat
     4861 ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga aaagaaaaac
     4921 agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga aaaattcttc
     4981 tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat cgttgttgaa
     5041 aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga aaaaaccgaa
     5101 aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa gccgatcatt
     5161 cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa acacagcata
     5221 atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag cagaaactgg
     5281 gaaatctggc aagctgagga tggttttgaa gggattattg agataattca ttaggtgatc
     5341 aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt gcaaatgtcc
     5401 gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca gactgatcag
     5461 tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg aatttttcat
     5521 attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata ggcaacccag
     5581 gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata ttattgcgta
     5641 tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct tctttttgca
     5701 gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg atgtagatcg
     5761 gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca ggaagaaggc
     5821 cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca cctttttgaa
     5881 tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag cctaccacta
     5941 catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag tcaatcaatc
     6001 ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata aaatcgattg
     6061 ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac tcatctgcta
     6121 gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac aagaaaggta
     6181 attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt tgtgtcccac
     6241 gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat ccactactct
     6301 tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg ttgttcttgt
     6361 ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat aaccggtttt
     6421 ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt atggtttttt
     6481 ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc gatgacaaaa
     6541 tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc gattcgctga
     6601 tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag ttgttcataa
     6661 ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg ttctccaagc
     6721 ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct ttcacctatc
     6781 aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc tgctttaggc
     6841 tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt ttcctgttca
     6901 gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg ttcattttgt
     6961 tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta tttgattggg
     7021 tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa caaaaattca
     7081 ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg atctacggta
     7141 gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac ttgatggtta
     7201 atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc gcgaaatccc
     7261 attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc taaaactaag
     7321 cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg cggcaacgca
     7381 gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa cagaccactg
     7441 tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc tacttggata
     7501 aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc tacttctttt
     7561 tcttttaata attcatgccc acgttctaga taactattta tttcttgttc atcttctgta
     7621 aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat ttttttctct
     7681 tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc ggctgctatt
     7741 tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga tcctaaaata
     7801 acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc gagatgactc
     7861 atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg caaaatatca
     7921 taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg agcagtaaat
     7981 gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg tttcgtccat
     8041 gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc aaactcagca
     8101 tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt gtttcctatc
     8161 gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc attcgttgct
     8221 tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat cgttgtcgtt
     8281 ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat ccttggccaa
     8341 ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc catgtaccct
     8401 ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg aaacaagctc
     8461 tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc gataaatcag
     8521 tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa aaaaacagac
     8581 ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc ttagtgctgc
     8641 ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt cggcaaagaa
     8701 tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt ttcgctggcg
     8761 gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa tgccaccttc
     8821 tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg taccggctgt
     8881 tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca aaatcatcgc
     8941 gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt ccaagcgcca
     9001 gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg tagtagcatc
     9061 ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca atacaaccac
     9121 tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca atgcaaggcg
     9181 catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc cttccctgcc
     9241 tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa cttttttttc
     9301 tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac ttgctcttta
     9361 cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt cttttgcaat
     9421 tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa ccagcctttt
     9481 gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc actatctttt
     9541 taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc acgtcgaatc
     9601 aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat ttcgctattc
     9661 tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa taaaccgtga
     9721 ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg attttgatac
     9781 gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg tccctttgtt
     9841 tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc acagccgtac
     9901 tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa aagatagtaa
     9961 gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga tcattcaaga
    10021 attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc ttcatcgtaa
    10081 tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc taaaaaaagc
    10141 caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa gatagattaa
    10201 aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt gttcaaaagc
    10261 tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta catcttcggt
    10321 tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt tgatttttgc
    10381 taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga tttctagtaa
    10441 tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc gagtagtcat
    10501 atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta cactggcagc
    10561 ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta tcagctcctt
    10621 tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca ctcagtgaaa
    10681 tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc caaatccgac
    10741 aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt gaaatgtttc
    10801 tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta tattctctat
    10861 cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt tgaaatctcc
    10921 taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta actcaggaac
    10981 ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat gaaaatcatt
    11041 tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac ttttttttcg
    11101 tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg caacaaaatg
    11161 tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac cagctacgct
    11221 tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct caatcgttac
    11281 tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg caaacgtaca
    11341 agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag acaaaatctc
    11401 ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta ttgcatttgg
    11461 atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc gatcaaaaaa
    11521 gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg cgttcgaaac
    11581 tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga tctcattcgt
    11641 cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac caccgggagc
    11701 cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc caacgatcat
    11761 tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact ttccattgac
    11821 agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc tatggtaagt
    11881 ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt ttttcacgct
    11941 tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa gacgatattt
    12001 tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt ttcgttagac
    12061 attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca tgttgctgcc
    12121 taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc gtttttatcc
    12181 caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg caaacttccc
    12241 aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt gtgacctttg
    12301 acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga ttttgcaatg
    12361 atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac aatgcttaaa
    12421 acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat ttcttctaat
    12481 atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta tgtagaacaa
    12541 ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc ttccacgtat
    12601 accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc catc
//
LOCUS       DAYYAR010000086        12434 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000086 DAYYAR010000000
VERSION     DAYYAR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.086"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..551
                     /locus_tag="ACHAT0_001965"
     CDS             78..551
                     /locus_tag="ACHAT0_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8451895.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            560..787
                     /locus_tag="ACHAT0_001966"
     CDS             560..787
                     /locus_tag="ACHAT0_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451896.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1423..1794
                     /locus_tag="ACHAT0_001967"
     CDS             1423..1794
                     /locus_tag="ACHAT0_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451897.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2050..2292)
                     /locus_tag="ACHAT0_001968"
     CDS             complement(2050..2292)
                     /locus_tag="ACHAT0_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8451898.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2324..3226)
                     /locus_tag="ACHAT0_001969"
     CDS             complement(2324..3226)
                     /locus_tag="ACHAT0_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8451899.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3239..3427)
                     /locus_tag="ACHAT0_001970"
     CDS             complement(3239..3427)
                     /locus_tag="ACHAT0_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8451900.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3441..4004)
                     /gene="amaP"
                     /locus_tag="ACHAT0_001971"
     CDS             complement(3441..4004)
                     /gene="amaP"
                     /locus_tag="ACHAT0_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8451901.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4042..4935)
                     /locus_tag="ACHAT0_001972"
     CDS             complement(4042..4935)
                     /locus_tag="ACHAT0_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8451902.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5013..5936)
                     /locus_tag="ACHAT0_001973"
     CDS             complement(5013..5936)
                     /locus_tag="ACHAT0_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8451903.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5985..6335)
                     /locus_tag="ACHAT0_001974"
     CDS             complement(5985..6335)
                     /locus_tag="ACHAT0_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8451904.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6368..7270)
                     /locus_tag="ACHAT0_001975"
     CDS             complement(6368..7270)
                     /locus_tag="ACHAT0_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8451905.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7263..8120)
                     /locus_tag="ACHAT0_001976"
     CDS             complement(7263..8120)
                     /locus_tag="ACHAT0_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8451906.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8454..9263
                     /locus_tag="ACHAT0_001977"
     CDS             8454..9263
                     /locus_tag="ACHAT0_001977"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8451907.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9303..9800
                     /locus_tag="ACHAT0_001978"
     CDS             9303..9800
                     /locus_tag="ACHAT0_001978"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8451908.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10185..10258)
                     /locus_tag="ACHAT0_001979"
     tRNA            complement(10185..10258)
                     /locus_tag="ACHAT0_001979"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10222..10224),aa:Ile,seq:cat)
     gene            10445..10768
                     /locus_tag="ACHAT0_001980"
     CDS             10445..10768
                     /locus_tag="ACHAT0_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451909.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10932..11186
                     /locus_tag="ACHAT0_001981"
     CDS             10932..11186
                     /locus_tag="ACHAT0_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451910.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11256..11501
                     /locus_tag="ACHAT0_001982"
     CDS             11256..11501
                     /locus_tag="ACHAT0_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8451911.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11577..11942
                     /locus_tag="ACHAT0_001983"
     CDS             11577..11942
                     /locus_tag="ACHAT0_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8451912.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12003..>12434)
                     /locus_tag="ACHAT0_001984"
     CDS             complement(12003..>12434)
                     /locus_tag="ACHAT0_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451913.1"
                     /translation="IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDH
                     SDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVL
                     LNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         3579 a         2316 c         2129 g         4410 t
ORIGIN      
        1 tttttcgata aaaaatagtt gttttacaat aattattctg ctatatttta gctatcaaag
       61 ataaaagagg gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta
      121 taagtgccgt atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa
      181 aaggattcaa ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc
      241 tcctagggat cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc
      301 aagctttttc cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta
      361 ttggtttcgc cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg
      421 ctccaggttt gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat
      481 taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt
      541 tgacagtgtg aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa
      601 aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat
      661 tctaaaaaat ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt
      721 tttggagtgt caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga
      781 agaataaaat aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga
      841 gacttttaga agttatttac gtctattatt cagatttttt atttcgattg aataccgttt
      901 tatctataaa taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact
      961 atcacttttc ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat
     1021 aatactttat tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt
     1081 attacaggcc attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg
     1141 tgttaactct tactccattc ttttttagta agctcccata tatactgtaa agccttctat
     1201 atattatgtc atgttgcttt ttaacattta ctatgtagtt ttgataaaat gatttgaata
     1261 acagtgatgg acaattcttt tttattttta acattatttt gataataatg attatttatt
     1321 taaaaataca ttagataaca tttgttacga cacgaaaaag cttcacaaac aaatatcgtt
     1381 attttaaaat gagggcgaac tcacacaatt aggaggaata gtatgccatt tattgtttac
     1441 ttttttcttt cattactcgt gatctatatt ccattaccaa catttttcct gctcgtcaat
     1501 cgtctagcag agcaacatga tacgattgct gctcttacga aactttgttt acttctattt
     1561 gtttgtctag attactttac gagctattat tggctaaaac agacaagagt aaaaaagaga
     1621 tattatttct tccttgtctt tttcaatctc tttgagtttc tgatccattg ctttttactt
     1681 catcagtatc atacgatcca tttgtggatc atctgggttt tcaaagtggt cgtattattt
     1741 tgtatgttca cctttcctat ctcaagaaaa tttagggagg cattgtttta ttaaagaagc
     1801 ttctctgtat caataaagac acatttaagg aaaaactttt aaaaatctaa ttgggaattt
     1861 gcccgcataa ataagaggct gtgacaaaag tcttgtcaca gcctcttatt ccgtataaac
     1921 agtgttcaaa agcttgcatc ttcgataata tgcccaacca aatgaaaaat atgagaagct
     1981 atttttgttt ggctgttctt attacttgat gaagaacagc tttttcacga cctcttttta
     2041 aaagtctttt tacagttttc gagcaaagaa tgatacgatt agtactaaaa tgatggcacc
     2101 aatcaatgat ggcacgattg ccattcctgc aatacttggt ccccaagaac ctaataatgc
     2161 ttgaccaagt gctgatccaa ctaaacctgc tacgacattt gcaataatcc ccatcgaaga
     2221 acctttattc gtgattgctc ctgcaattgc tccgataatt cctccgacga taagtgacca
     2281 aataaatccc atgtttcgtt cctcctaatg ttttacttcc ttattactga acccgtggtt
     2341 ctgtattttc ttttactttt tgtgcgccgc ttccagctgc tgattttact ttatctgttt
     2401 gattcgaagc aaattcaccg gtttttttcg ctgcgtcagt tactttgtct tgcaccgttt
     2461 cttgatcttt ttcaaattct tcttttgttt tgatgtccac tacgttagcg ttgacttcga
     2521 tcacttctaa atgtgtcata ttttttactt ctttgccgat tacttcttgc atttcttcaa
     2581 agattttttc gatatctttg ccatattcca caatgacatc catatcaact gctacttgtt
     2641 ttttaccgac ttctgtttcg atcccagccg ttacattatc tgtattcaca agttttcctg
     2701 ccacattaga gaagaaacca ccgtttgcag ataataaacc gtctacttgt tcaattgcga
     2761 ttccgatgat tttttgcaca actttatctt cataattcag ctcaccgcgc attgaatctt
     2821 cctgttcaga atcatgcttt ccttggttat ttgtatgatc agaaccaagt tttccttgtc
     2881 cgtttgtgtg gtcagaacta agtttttctt gtgctttcgc atggtcagaa ggatgttttt
     2941 gctcttccac aggtttagaa ggatcgattg catcatgttg ttctggaact ttagattctg
     3001 gagttcctgg atgatgaggt gcatgctttt ctgcatcatg tttcttctct tctactcctt
     3061 gttgaacgtt agggactact ggatctggat gtgcagatac tggtgctgtt ttgttctttt
     3121 catcgttcac accatttgat tggtttggtg tcgttgggtc tggatgtgca gacactggtt
     3181 tttggttgtt cattttattg ttattatttt tgttattgtt attcatggat gtttcctcct
     3241 aagatttaaa atagtgatct aagattttcg tttgtttcag ataaaaaccg atagcgacgc
     3301 cgatcgctgc catgatcaag atcaataagg tcttgaaaaa gcccacggtg atccataaaa
     3361 cggctaatat caatccaagt accccaaaga tgatagggag cttgtatgtc gaaaatagtt
     3421 ctttcatttt tggtcccccc ctattctact cgtttgcggt cattgcttcg tttagctggt
     3481 tcttcaaagc ctgtgtattt caccgatact ttcactggct cttttgttcc caatatgtgt
     3541 tgtacttcat tttcgatttc tttcatcaat gttcctgttt taccaatgag tgctgaagtt
     3601 cgtttcaact ccccttttac attgacggcg atcttgtttt tcgttgcttt gacatttact
     3661 tttggtgaat ggatgaattc atcttcatgc aagtgagaac gaaccattcc ttcaattgct
     3721 tttttatcca acgtcaactc cccatcacca tgtttcagta gaaagtttcc gattgttttt
     3781 ggataaaaca ggaaaacaag taacgtgata attgtcaaaa tagcaaatgc aacggcaagc
     3841 caaaatacat attgttgcac gtatgaatta gtgatggaaa agaatcgaaa cttctctagc
     3901 tgaaaaggca atggataggt tgcacttaat agtatcgcaa acaaaagcac agatagcaaa
     3961 agtaatgttc cgatcactcc gatcgttttt acacctttat tcattttcgc acctcctcat
     4021 aggggtgaaa cgatcaactt cttataagtt gatcgcttct ccaccagtta ctccgtaaat
     4081 ttgggctgta acataacttg ctttattcga tgctaagaaa acataaactg gtgctaattc
     4141 tcctggctga cctgcacgtt ctagtaaact ttgctgacca aattcaggga tcgaatcaga
     4201 aggttgtcct tcgtctagtt gtaatggtgt ccagattgga ccaggtgcta caccatttac
     4261 acggattcct ttaggtgcaa gctgttttgc taaaccgatc gtaaagttag cgattgaagc
     4321 ttttgttgct gcgtaatcta ataaatgttc gcttggattg aatgcttgaa cagaagttgt
     4381 tgttacgatt gctcctcctg ctgggatatg cggaacagct tctttcacta aagcaaacat
     4441 agcgatgatg tttatcataa atgtgtcttt gacttgctca agtggtaact cagcaataga
     4501 ctgacaagaa atttgctgtc ctgcgtttag taccatcgta tctagcccgc caagttcact
     4561 cacggcttta tctatgattt ctttaggtgc ttgttcgtca cgaagatcaa aaggtagtaa
     4621 gacaatctta cgtcctgcaa ctttcacgta ttttgctact tcctctgcat ctttttcttc
     4681 tcctgggaag aattgaagag cgatatctgc cccttctcta gcaaatgcaa tcgctgctgc
     4741 acgaccgatc cctgaatctc caccagtgat tagtacacgg cggttttcta gttgattatt
     4801 gccttcatag gattcttctc cacaatcagg aacaggaatc atattctgct gtaaagctgg
     4861 tgtatcctga tcttgtttag gaaatttctc cgtatggtac aagtttcgcg ggtcgattaa
     4921 ttttggttct gtcattgctg tattcctcct agtttttaaa ttgtattgcc aaaacaaatt
     4981 tctttgtttg atacctgttt taaatagatt ctttaatttg tataatcttt ccggtataaa
     5041 tggtaaccta aaactactgc tgctatcaaa gagccaaaga ctacgagtat aaagcctaga
     5101 gcagaacttt tacccggtaa tcctcctgta ttcattcccc aaatccctgc gaacaacgcc
     5161 ggaacggaaa tgattaatgc agctgaatct agatatttca tgatatgatt tagattattg
     5221 tccatcatcc cgttgaatag atcaccaata ctctctaata aatctcgata gatctcgatc
     5281 attttttctg tttgtcgctg tctcaaatgg acatcataga gtagctgttc atcgtctaaa
     5341 cgtttgatga aagtttcaga attccataat tgatccattg tttcccgttg atctcttaag
     5401 gtatgatcga tatatacgat gtctctttca agatctgcct gtttatacag ttcttcattt
     5461 tccgttgttt tacgagctgc tttatccaaa gcgtcaattc gctttttcat ctctcttagt
     5521 tctttcacgt aatgggtgta aatatctaat aatgtatacg tgatgatctt ttcaaacgag
     5581 tttaaatgat ttccatgctt tgtcagcagc cgatccacaa attcggtttt tctatcgata
     5641 caagtaatca agagattatc ggaaagtaca aaagatattg gcgttaattt tttttcgata
     5701 cgattatctg actgatctag attaatcact accaaagaaa atggatgctc tagtttcgtc
     5761 tcataaaggc gctccatccg agaaacttct tccggatatt cggttcctac gaaaatatca
     5821 tcaggaagct cgaattcttc gattgcttgc tcaatctctt cgggctttgc actatcaagt
     5881 acgagccaat tatatttttt ttcatctgtt tctattacat tcttttcttc atccacaaag
     5941 tagtatctca atgtatacct cctttttgga ctttctcgaa actattagct gaagaagcag
     6001 ttctttaatt tgatattata atcgagtact gcgatttcat tttctaatga aaagctgtca
     6061 agctgtttgt tgaagtccat gttcaatcgt ttaccgaaat aattttgctc taatttagat
     6121 gtatagatgt ccaataatgg atgctcgatt ttagtcgcat attctccaaa ttccgaaaca
     6181 aaaggaatga tttgaaaaaa gctgcctttt gcaaaagcac ctttgtccct tagaaatgga
     6241 tcggaatcat tgataagcgc gagtgcgatt tttgtatttt ccccaacctt ttcaattaac
     6301 gtctctactg catttttaga cattgtaatt tccattccta ctccccctct tcagttgttg
     6361 tcttctatta tttaacaact acatctgttt ttggttgaac ttttgcatat tttggatatt
     6421 tagggaattt agctacacaa cggaaagccg tcatccctgc atatttttcg taatatttct
     6481 tcgttggccc ttcaaaatcc ttgctccacg gtttcacatg tcctgaaaaa tggataatgt
     6541 aaggattgtt gcgagtttct tcgtattcac gttcacctgc tgctgttgga tgtttttttg
     6601 cttttgccat gatatagcct tgtgcattcc aacgcggatg aagtggtaac caacggtcat
     6661 gtaagatagc atttaatgca tcttggtcat ggaatcgtaa tttttctgga ttatgttcga
     6721 tgaaatctaa tactttttgc gtaatatttt catctaacca ttttttcaca ttgatcagca
     6781 tcaaacctga gttgaaataa cgcattgatt tagctgaaat tttcattttt tccagtcgtt
     6841 gatggaaccc agcgtcttca acagctgcta cgattgaatc accgaagtca agacgccata
     6901 atttagagat atcttgtaaa gcgatcatgt cgcaatccat atataagata cgttcaactt
     6961 ctgtgccgcg gaacaattcc ggaatcgcaa tacgataata agctgtttct ggaatacgat
     7021 cgctgattac cacatttgta aagaattctt tattgatttt caagaattcc acatctgagt
     7081 tcattcgtgc gtttttcact gagaagcgta aaagctgttt actttcaaaa tcgatatcat
     7141 cgtcaatcac atagaaccga acaaatttag acgggttgca attatctaat actgagacaa
     7201 ataatgcagc taaatgtggt acgaattttg tgttacaact tgcgactact gctatttctt
     7261 ttttattcat tgatctcgcc tactttcgtt agagtactgt gtgctaattt ttttaagtat
     7321 aaattggtat acggatgatc tttcaacgta ttccaaggtt tatcatgtcc tgtaaagtga
     7381 acgatcgaag gtttttcatt tccttcttta tactgacgtt catatttttc atttggtgct
     7441 ggatgacgtt caaaaatcag cgaagtctgc atgttccatt ttggatgaag ttgttcccaa
     7501 tcttcataaa gtacagcatt caaagcatct tggtcgtgat agatgattcg atcgccattt
     7561 tcactgaggt aatggatagt tttttctgta atttcttttt tattccattg atcaatgtca
     7621 atcaccatca ctcctgaatt gaagtacaag tcatcggaat cgattcctaa tctttccaaa
     7681 gctttcactt ggcctggatc gataatagcg ccgattgttt ttccatttaa cggctcatca
     7741 tatagtttta cgatatcgtc tagtactaac acatctgaat ctaagtaaag gactttctta
     7801 taatcctctt tagctaataa gtttggtaaa gaaattctta agtaagcggt agttgtgata
     7861 tggtcactga ctaggaagtc ttcgtagata tctttttcca ctgtcaggaa ttggatactt
     7921 gcattacttg aatatttatt taccgtttct tccaatcctt ctttactata ttctgataga
     7981 ccatcgtcaa tcacataaaa ttttatgcgt cttgttttat tgcaattttc taatgcagtt
     8041 gcgatcatca cgctcaagta aggagcataa ttttcgtctg aagcagttac cacaggaact
     8101 acgccgtatc tcttctccat caaaatcact cctttttcta atctttttct aacctgttct
     8161 acatctagaa tattcctcat ttggttttaa agcaaaatat atgctttgtt ttcattgaat
     8221 attttaaaat ttttttatca caaaacgcat cggtaagcgc taactattcg tttttcatgg
     8281 ctttttattc aaaggtattg accaaaaact actatatata gccacctttt tcttaagaat
     8341 gttttttgaa aaaattcata atgacaaagc aagttagttt cattttttta aattctcatt
     8401 taatcttaag gtaacaggaa aatagatatg taaaaagaaa gtaggaagat aaaatgaaac
     8461 tagcagcgat tgatttagac ggtaccttgc ttgatcgaaa tggaaagata ccagaagaaa
     8521 atatccaagc acttcggaaa tttgatgaaa atggcggaat cgtaaccgtc gctaccggaa
     8581 ggaacagcat ttctgctaaa gatatttttt ccgaattagg aatcggtggc tatttgattt
     8641 cttccaatgg ttcactgatt gctgaaatga aggatggaaa aatggcgaaa gttctcagac
     8701 gttcaaaaat agatagcgat acactgagaa aagccttctt ctatgctcaa gaagcgaaaa
     8761 tatccatcat tgctagccga gaaacccaag atgatcagct gacattcgat gaatcaaatc
     8821 tagtcaaaga tgacccttat tatgaacatt tcaatttaca gaaccattct tttgatcaaa
     8881 ttttaaatat gttggaagat ccttctctta gctatcttaa attagcatta acagataaga
     8941 gtgaagaaaa actacagaag ctacaaagag atctgcaagc agatggtatc caatcagtat
     9001 tctctgatcc gcatttctta gaaatcacgc ctacaggtat cactaaagct cattcacttg
     9061 tttttctggc ggattacctt ggaatcgaat cgagtgacat catggctttt ggcgatcaag
     9121 aaaacgacat tgctatgttg aattacagcg ggttgagtat tgcgatgggg aatgcacagc
     9181 gacaagtaaa agaaattgca aaccaagtaa cactgacgaa tgaagaagct ggtgttgctt
     9241 atattttaaa aaacttctat taaaaagaat aaaatgacga tatgaatgta ggaggaaaga
     9301 aaatgttagt tattattgat atgcaaaatc atattcttga tccaaacagc gaattttata
     9361 ttccagagtc agatcaattg gcagaacgta ttgctcaaag attagctaaa gcacgccaaa
     9421 ataatgaata tattctcttc acacgggata taccagttga actgaaggac aaagaagaag
     9481 aacgagaaga tttgcaattg atccctcttc tttctccaca agaaaatgaa cgagtgatta
     9541 aaaagtatta ctttacgatt ccacctgaaa ctttagctga aatccaggaa actttatttg
     9601 aaagcaaaga agaacaaaaa gagatcgaag tagtcgggat tgaaacaaac ttgtgtgtac
     9661 tctctaatct gataggcctg cagagtgctt ttcctgaagc agactttttt gttgatccaa
     9721 tgttagtctc cagtagaaaa cacggagaat ccgctttaga gctgttgaaa gatttcaacg
     9781 tctctatcgt cgaatcataa aaatatctat cttcatttac gatatttaac acacaataaa
     9841 aaaagcacgc agaactgttt tagtcctacg tgctttttct atcaaagtag attggctttt
     9901 ttatgacaca gagtgaaacg gcaggctcac gacctctttc ttaaatggtg ttcaatcagc
     9961 tgaccctcgg tattagctta acaaacggca aaatatgagg aactattttt ccgtttgtta
    10021 agctaatact caggtcaaaa cgctgatttc acaacctctt ccttaaacgg tattcaaaag
    10081 cttgcatctg cagtaataag aacagccaag taaaaacata agaagctgtt tttacttggc
    10141 tgttcttatt acttgatgca gggcagcttt ttcacaacct cttatgtacc ttgtaggact
    10201 cgaacctacg accggacggt tatgagccgt ctgctctaac caactgagct aaaggtacat
    10261 cataaataaa atttattttt gttacttttc aactatagca cgaatgacaa actctcacaa
    10321 gtttcaaaat ataagtaaaa taatattgtt attcttattt cttttacttg tgtcataact
    10381 attttgttac tttaattttt atttgcttat tttagaaaaa atgttattct tttatttatt
    10441 ctcaatgtta ggagtgatta ttttgccatt tattgcgatt ttattcgatt tagttgcggc
    10501 agcagcttat tttctccaat taaattatca aacctcagga attctgttta ttggaatgat
    10561 ttttcaaggt gtgattaccc ttttgcttct tgtcatgatg ctcagctata aaggaaaaag
    10621 atatgcccgt attcaaacaa aagtatttgt taaatatgtt tccattcgct atgggatcat
    10681 cattctttcc tttttagtga atgcgatcgt cctcttttta tatgtattga attacttaga
    10741 tatcaatcca ttgatttttt ctcgatagat aagacacatt cagcggaaca gttcctgctt
    10801 atcagcagaa aactgttctg ctttttcatt tttcattgat ctattcttct tttattttcc
    10861 tagcatttca tttgtgatgt atgcaataac atgttatctt ttattatatg aatcgttgag
    10921 gagtccgtta catgattacg ttggtgagag ttttgttttg ggtgcctgcg gtcgcactgg
    10981 tcgcaagtat tgtttatctt atgaattgga acaaagaacg gttttattta gctattttga
    11041 ctcttccggc tatctacttt atgtggaaag tttttaatta caactacttt gaacccgata
    11101 gtgtttttat agaagaatta tcaggactgg ttttgtcttt attgatcgtc attctttatt
    11161 tgatacgttt aaataaaaaa cattgagtac tattgattgc atgtgtagct ttctatagtc
    11221 attataacta ttctactata atgaaaggtg attaaatgag tatcgaagaa gctattttac
    11281 tggatacttt acgttgtcgt ctgaatttct taagaactgg ccgatcttta tctcaagtag
    11341 ctgtttctag cgcgttaagt attcctttat tcaaatatcg taagtacgaa gaaggagaag
    11401 aaattccaag catcattgaa ttaatctata tcgctgactt ttacgatatt tccgttgatt
    11461 acctaattgg tcgctcagaa acagcaagca aactgaagtg attttttcca gccttggtgc
    11521 tggttttttc atttaacgtc taaggttaac aatctatata taaaggagac atttctatgc
    11581 gattatggca tgaaaaattg atttcaaagc ttcctcgtca acagctttta ggacaacatc
    11641 gggaatgctg tgcattaaga ggaaatggct ggggaaagaa acatgctact gtggattatg
    11701 tttttcacta ttctccttat agattattcc aatatcatca gcttgtcatg gatgaaatgg
    11761 aaaaacgtgg ttacagccca gcaccagagt ggaaaaatcc tatgtatcgt ggcaaatcat
    11821 gcgaagcatt ttttgattta cctactgtag agcgcaacgt gccgatttat ccggaacacg
    11881 atgaagttta tctagcagaa tgtttagcaa atttggagca aaaaggaatt tatcttacct
    11941 aatataacta gaaaagcctt ctatctcctc ttacttcatt aaaaagcttt aaccatctta
    12001 gatcaagcta aaatgtcgta agttttaggg ttatcttcat cctcacgtgc aaaactaaaa
    12061 gcgtactcta gttttggttc atcgccaact tccctagaca taaatgaaca tctcctgacg
    12121 tggtttaaaa gtaccgcatc ttcgacgtct gtatacgatg cacagcacag attgcttctt
    12181 ttgatctttc ttttgatttt tctgttgctt cttctacacc ttcttcgatt actacatcga
    12241 tgcttacaat tgcttctgcc taatattccc atgcatcgtc gatttaagcc tctgtagtca
    12301 ctgtgatcgt ttccacattt taactcctct gtcgtggctt cagttgattt tgtgatcagt
    12361 tcagttgcta agccagagtc agaacaacct gccattgcta gcgttaatat tgatagtaaa
    12421 aatagttttt ttat
//
LOCUS       DAYYAR010000087        12144 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000087 DAYYAR010000000
VERSION     DAYYAR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.087"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACHAT0_001985"
     CDS             <1..566
                     /locus_tag="ACHAT0_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8451914.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /gene="fic"
                     /locus_tag="ACHAT0_001986"
     CDS             967..1572
                     /gene="fic"
                     /locus_tag="ACHAT0_001986"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8451915.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="ACHAT0_001987"
     CDS             1588..2160
                     /locus_tag="ACHAT0_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8451916.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="ACHAT0_001988"
     CDS             complement(2412..2669)
                     /locus_tag="ACHAT0_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451917.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="ACHAT0_001989"
     CDS             complement(2795..3610)
                     /locus_tag="ACHAT0_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451918.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="ACHAT0_001990"
     CDS             4127..5452
                     /locus_tag="ACHAT0_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8451919.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="ACHAT0_001991"
     CDS             5445..5795
                     /locus_tag="ACHAT0_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451920.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5792..5962
                     /locus_tag="ACHAT0_001992"
     CDS             5792..5962
                     /locus_tag="ACHAT0_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451921.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACHAT0_001993"
     CDS             complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACHAT0_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8451922.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="ACHAT0_001994"
     CDS             complement(7113..8066)
                     /locus_tag="ACHAT0_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8451923.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="ACHAT0_001995"
     CDS             8678..10171
                     /locus_tag="ACHAT0_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8451924.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="ACHAT0_001996"
     CDS             10306..10602
                     /locus_tag="ACHAT0_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8451925.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="ACHAT0_001997"
     CDS             10703..11554
                     /locus_tag="ACHAT0_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451926.1"
                     /translation="MKSDDWIEHNWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11787
                     /locus_tag="ACHAT0_001998"
     CDS             11575..11787
                     /locus_tag="ACHAT0_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451927.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4172 a         1863 c         2268 g         3841 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca actggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaa
//
LOCUS       DAYYAR010000088        12123 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000088 DAYYAR010000000
VERSION     DAYYAR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.088"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..249)
                     /locus_tag="ACHAT0_001999"
     CDS             complement(85..249)
                     /locus_tag="ACHAT0_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451928.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            544..1872
                     /locus_tag="ACHAT0_002000"
     CDS             544..1872
                     /locus_tag="ACHAT0_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8451929.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2074..2907
                     /locus_tag="ACHAT0_002001"
     CDS             2074..2907
                     /locus_tag="ACHAT0_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8451930.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2923..3660
                     /locus_tag="ACHAT0_002002"
     CDS             2923..3660
                     /locus_tag="ACHAT0_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8451931.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3815..4783)
                     /locus_tag="ACHAT0_002003"
     CDS             complement(3815..4783)
                     /locus_tag="ACHAT0_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8451932.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5015..5848
                     /locus_tag="ACHAT0_002004"
     CDS             5015..5848
                     /locus_tag="ACHAT0_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8451933.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5882..6721
                     /locus_tag="ACHAT0_002005"
     CDS             5882..6721
                     /locus_tag="ACHAT0_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8451934.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6747..7307
                     /locus_tag="ACHAT0_002006"
     CDS             6747..7307
                     /locus_tag="ACHAT0_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8451935.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7506..9227
                     /locus_tag="ACHAT0_002007"
     CDS             7506..9227
                     /locus_tag="ACHAT0_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8451936.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9392..10534
                     /locus_tag="ACHAT0_002008"
     CDS             9392..10534
                     /locus_tag="ACHAT0_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8451937.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10550..11761
                     /gene="thiI"
                     /locus_tag="ACHAT0_002009"
     CDS             10550..11761
                     /gene="thiI"
                     /locus_tag="ACHAT0_002009"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8451938.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4130 a         2034 c         2520 g         3439 t
ORIGIN      
        1 ttttattatt atgatgaatc aagctttctg tttttgtcac gtatgttgta atctttacga
       61 aagagtagag ttggctactc ataatcaacc atacttccca tcatcgctaa atacgaaata
      121 aatcagcatt cctacaccaa tcaaagcaca agctgcacca agaaaaggga tgacattccc
      181 aaagttatct gtattcataa gtatgccacc acccacaaac aaaaagccgc cagcaatcag
      241 gtttttcatt ttattcatct cctttcttac gttatagtat ttccttcttt caatgagtat
      301 tttgaaagct atcttattgt atgatgttct tctaggcctc ttctttatta tacaggccat
      361 tttcgttatc tcatagaaat attccttttt atctgaaaac tattgtatac cgtatgacat
      421 ttttcttcat gaagttgtaa gtatattctc attcaaatga aaatttcatc atagaagaga
      481 ttgtatttct aaattcttaa gagtaaaata cgaagtataa cttggatttg gaaggatgat
      541 tggatgaaag ctattacgcc tgatatgaca acaaaattta acaaacagtt tcaagcaaat
      601 atcaaacagc aggcattgca gcgggcagtt gtgaagaacg gaattactgc ttctgctgaa
      661 aatgtttcag cacatgtgga gaatacaccg gtattttcta tagatttggc tacggggaaa
      721 gtggctaatc aaatgcaatc ggggcgttgc tggatgtttg ctgcactgaa cacattccgt
      781 cataagatgc tgaatcaatt ccatttaaaa gagtttgaat tatcacaaaa ttacactttt
      841 ttctgggata aatatgaaaa ggcaaattat ttctatgaaa acatcattgc aacaggaaaa
      901 gaggcaatcg atagccggaa agtagctttc ttactcgcta ctcctcaaca agatggcggc
      961 caatgggata tgatcgtttc attattccaa aaatatggcg ttgtgcctaa atcggtgatg
     1021 ccggaaagtg cgaatagttc aaattctcga gatttgaata actacttgaa caaattactg
     1081 cgtaaagatg cagtagtgtt acggaaaatg gttgctcaag gagaaacatt agacgctatt
     1141 gaagaaaaga aagaagaaat gctagaaagc atttataact tcttagctat ctcattagga
     1201 acacctccaa aagaattcga ttttgagtac cgcgatgaag aaaagaacta tcatcttgac
     1261 cgaggactga caccacaaat tttttatgat aaatacatcg gtgtaaaatt agatgattat
     1321 gttagcgtga tcaatgcacc aacaaaagac aaaccattca atcgttctta tacagtagag
     1381 atgctaggaa atgtcgttgg cgggaaagaa gtcaaatatc tgaatgttga tatggaaact
     1441 ttcaaaaaat tagcgattgc tcagcttgaa gaaggagaat ctgtttggtt tggttgtgat
     1501 gtaggtcaat cttctactag aactagtggc atcatggcct tagatgctta ttcaatggat
     1561 gatctatttg atacggattt tacaatgact aaagctgaac gtttagattt tggagaaagt
     1621 ctgatgaccc atgcgatggt attgaccggt gttgacttag tagacgggca atcaacaaaa
     1681 tggaaagtcg aaaatagctg gggcgaaaaa gttggaaaga atggcttctt tgttatgagt
     1741 gatgcctgga tggacgaata tacgtaccaa atcgttgtaa gaaaagattt attgacgaaa
     1801 gagcaattaa atgcgttcaa tgaagaacca attgttctat ctccgtggga tccaatgggt
     1861 gctttagcat agacgagtat ttcaatgaag agtatgtagt aaagataaaa agagctggct
     1921 taagccagct ctttttatct ttaaagtcca ttccctgttt tttatggtcc ctgtgctata
     1981 attctaaata aaggataagg attggaaata atccttttat cattaaaaat atgatgacga
     2041 agtagaataa aattgggagt aggggggaaa gtcatggata tccaaactgt tttatctgat
     2101 ctgaaattaa atggtaaagt gattccagtt gttggtggcg atgttaatca gacctatcgt
     2161 ataaaaaccg aacatcgtgc ttatttttta aaaatccatc cgaatgtaaa aaaaggattt
     2221 tttgaagcag aagtagacgg attaaaagaa ctgtcagcat tcgtacgtgt accggatacg
     2281 tatatgctag gtgaaacaag tgaaggcgct tatttgttaa tggaatggat cgagccggga
     2341 aaaggggatc aacgagatct tgctgcagct ttagctaatc tccatcagca aacagcgcca
     2401 caatttggtt ttcgtaaaga taactatttg ggtacacttg ttcagaaaaa ttcttttgaa
     2461 gaagattggt ggactttttt ctttaaagat cggcttgaat cccagatttc tttggcagaa
     2521 gaaacgaatc gatggaatgt acagcgtcaa gaaaagtatc tgcgatttaa agaacgtgta
     2581 ttgaaatcgg ttgaaccaaa aaagatcact ccacgcctct tgcacggaga cctgtggagt
     2641 ggtaatgtgt tctttgacca gcaagggcat ccagtttttg tcgacccagc tgtttcttat
     2701 gggaatcggg aacaagatat tgcaatgagc cagttattcg gtggtttccg accggaattt
     2761 ttagatgctt atcaaactat atttccatta gaaaagggat ggaaagaccg actgccgatt
     2821 taccagttat attatctttt ggcacatttg aatatgtttg gagaaagtta cggttcccaa
     2881 gtagatcaat tactagaaaa tttttaagag gagggcgtgt ttttggcaaa aaaatatacc
     2941 aaaaagcatg aagtttcata ttatgagtgt gacatcaacc aaacgatgac atttccttcg
     3001 atgttaggag tagttatcaa aacgtctgaa gatcaaagcg accagttggg aagaggcagt
     3061 gaattcgtca atagctttgg acttacttgg gtcatcacga attattccat ggagattaat
     3121 cgtctcccta gagtcggtga agtaatctca gtaacgactc aagccatgga gtacaataag
     3181 tttttctgtt atcggaactt ctggatccat gatgaagatg ggaaagaatt agtcaaaata
     3241 gaatctgttt tcgtattgat ggattttgtg aatcgaaaaa tgagcagtgt taatgaagag
     3301 atcattgctc cttttgagag tgagaagatc aagaaaatca agcgtcagga aaaaattgaa
     3361 aagattgatt cgggcttcat gttgccatat cgtgttcgtt tttatgatat cgatagtaat
     3421 cagcatgtta ataatgcgat gtattttaat tggatcattg acgtattagg atatgacttt
     3481 ttgactacgc atgttccaga aaaagtaatt attcgatttg ataaagaagt agagtacggg
     3541 aatgagatcg aaagccattt tgaacaaatc cctatcgaaa atggtgtcaa aacaaaacat
     3601 gaaatccgaa tgcaagaaca gctgtattgt gaagcaaata ttatatggaa aaataaataa
     3661 aaaagaggtt gtgacagaag ggggcaactt caagaaataa gaagaaattt acgaaaattg
     3721 tttctcaaat ttttgtaaat tttcgactta gttttcgaag aagctatttt tgtcacagcc
     3781 tctcttttta agtttagcta ttagataatg atgtttagtt ttggataaac gaaatcaatt
     3841 cttcacccga caaaccatag tttacagcaa tcaatagttt taaacgtgct ttttgactat
     3901 taatactgtt acagaaaata atccccattt tttctagttc ttttcccccg ccttcataat
     3961 cataaacagg ctctgcgatt ccattgaaac aacgagaaac aagtactaca gggatttttt
     4021 ggacaagaag cttttccaaa gcagctaagg cttgtggagg gagattcccc gcacctaaag
     4081 cttcgattac caaaccgtct actttcgtat gggcgatggc ttccaataag tccccttgca
     4141 tcccagcata tgctttaacg atcgggatag tcccatctac agcggaaatg tccagtcgct
     4201 ttgtttctaa aagttcctgc aagaataaaa ttcgattctt agttactaag ccgatgggac
     4261 ccaatgtagg tgttcggaaa gtagcaacat ttgtggtatg ggtctttgtc acgtagcgtg
     4321 cagaatggat ctcatcgttc atgaccacca aaaccccctt atctagcgct tcttcacaac
     4381 ttgcgacacg aatcgcactt tcaaaattat aaaggccgtc acttccaagt tcattacttg
     4441 aacgcattgc cccagtcaaa acaattggta atttgttgcc aatcgttgta tcaagaaaaa
     4501 aagcagtttc ttctaatgta tctgtaccgt gtgtgatcac cactccggat atacctgatg
     4561 cataagcgga caagatacgt tcttttaata ccaacatgtg agaaggcatg atgtgtggtg
     4621 agggaagatt gaagatatct tcaactaata attctacatg cttggggatt gtgatttctg
     4681 catctaataa tgggtttgcc acatcaggac taacaccgcc cgtgagcatg ttttctttca
     4741 tggcgatcgt tccgccggtg tgtagtacaa gtattttttt cattttacag acttccttac
     4801 gaataatttt gcatagccgt taaatcttat tgtatactat ataaaagagt tttgctatca
     4861 gtgaagaata acgaatagcc taacttatca taaaaaagct gaccttatta aaatggaaaa
     4921 gactcaacct tgacctccta atacaatgga gtatctggtt gagtttcttt tacaagaaag
     4981 cagccaagga aatcaatcac gaaggtggcc ggaaatgatc agaatcggat taacatcgtt
     5041 taaagaacat gacaaattaa ctggtaaaaa gaataacact ctttatgaat atgcagggtt
     5101 tctaccctta gtggaattag atactgccta ttatggaatt ccccgaaagt cctcagttga
     5161 gaattggata agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct atcaaggaat
     5221 cagcggtcaa ggagactgga agccttatta tcagaccgaa gaagagatga ttgcgcattt
     5281 tttagatgtt atgtcgccaa tggtagatgc tggaaagatg ttttgctttt tagtccaatt
     5341 cccctcatca ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac ttagaaagtg
     5401 gtttgagggt tatcctgttt ctatagaatt gcgggattat tcttggtaca gcgatgaatt
     5461 tcttgaacgt acaagaaact ttatgtctag agccgctttt tctttagcca tcatcgatga
     5521 gccacagttg ctgaatacaa cgattccttt tgatccattt gtcaccaatc cgaactttgc
     5581 tctttttcga ttccatggta ggaatgcgca aggatggcaa gcaaatgata aagagtggcg
     5641 taaaaaaaga acgttatatc gttattcaga aaaagaactg caagaactag cacatgaagt
     5701 caaagtagtt gattctaaga caaaagaggt aggtgtgatt tttaataata attctggtgg
     5761 tgatgcggct gaaaatgcat tagcactgca aaaaatatta aatatcaagc cagatggact
     5821 caatccaacc caaatggatc tgttttaagg gcttatttta ttcaagaaag gggcgaagtc
     5881 tatgatcaaa gctatttttt ttgatattga cggaacattg gtcaccaatc gttcaaaagc
     5941 gttggaatca acaaaacaag caattgaata tgctcggaaa aacggcattt tatgcggtgt
     6001 agcaacgggg cgcagcccag taaagatcaa agaaatcatt gatgaattgg aattagatat
     6061 gtatgtcgtg tataatggtc aactggtttt tactgcagat cgcacgatta tcgaccaccc
     6121 ttttgaacaa aaagtattgg aacatattgt tgaatttgca gatgagaacc atcgacaaat
     6181 cgtttttggt gcgagaaatc ggttagatgg atcaacgacg atgctattag gacaatcgat
     6241 ttttatcaag cgactagtca gctttttacc gcgtaaattc ccggtacgtc tgatgaaaaa
     6301 aatcctccag gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt tagagatatt
     6361 aaaagaacca atttatcaat gtatattgct cagtccggag tcagaacaga aaaagttaga
     6421 acatcgcttt cccgagtgta cgtttcagcg ttcaaatacg tatactgtag atatcatccc
     6481 aaaaggtggt tcaaaattac taggcatcca agctttcgca aatgctgtcg aaattgaaat
     6541 ggaagaaatt atggcattcg gcgatcatta caatgatatc gagatgctga aaggtgtagg
     6601 aattggagtg gccatgggga atgctcagat cgaagtaaaa catgctgcag actttgtgac
     6661 tcaatcaaat gaagaagatg ggatatacga aggactgaaa cacttcggtc tgatttatta
     6721 gtcaatgaaa gaaggaaaaa atcagtatgg aagcagagac accttttcaa aaagtagaaa
     6781 cattccacaa aacttttgat ccaagaagac cagagatacc cacggctttt acagaaaatg
     6841 aagctcatac acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt gcagctactt
     6901 cacctaataa agaacaactg catgaagctg ttatgctgct gcacgaagca ttagataaag
     6961 cagaagaaaa aatacttggt aaaacacagt ggggaagtga tccgctggtt gaacaagtag
     7021 atgcactgac agatatcctt tactttactt atggttcttt ttcattacta ggaatagtgc
     7081 ctgatcgaat attcgaaatc gtccatcagg caaatatggg gaaattattt ccagatggaa
     7141 aacctcatta tgaccctgta acaaataaag tgttgaaacc tgaaaactgg cagatggaat
     7201 atgctccaga acctaaaatc gcggcagaaa tccaacgtca gattcaagaa gcgattgcta
     7261 agaagaaatc cgctgatata ggcatgtgca caaatgaaaa agagtgatca gagtttcact
     7321 atttttgtta tctttatacg atggaaaaac aaaagatttt cgtttaagtt agtgaatgca
     7381 tgaaaatagt atgctataat ctagaataga tgtcttttgc acgagagacg tattatttgg
     7441 atttatgcca agcagaacgc catctgttgc ataaaagatg catactgaaa acggaggaac
     7501 cataaatggg aaataatgtt atcatcggaa tcgtcattgc aatcattata gtcgccattg
     7561 tcgtcttttt ggttagttac ttgatgaaga aaaaaaacca agatcgattg gatgccttag
     7621 aaaaacgcaa ggaagaatta tttgacttgc cagtgattga agaagtcgat gatgttaaaa
     7681 aaatgcatct ggttgggcaa agccaaaata cctttcgtga atggagtcaa aaatggaatg
     7741 acatatctac ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg gaaaacctca
     7801 atgaaagttt tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa gctacaatgg
     7861 accaaatgga gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg cgggaaacgg
     7921 aagaacgcaa ctcactagct gttcagcagg cacttgacgt ctatgaagaa ctgaaagaaa
     7981 aagtcagaga agacgagcat gaatacggac cagctctttt tgaattgcaa aaacagatca
     8041 aagtgatcga aaaagaattt acgcagtttg tgactttaaa cacttctgga gaccctgttg
     8101 aagcccgcga ggttttggat aaagcagaaa aacatacgta tgaagtggaa gctctgatga
     8161 aaaaaattcc accgctgtat gaggagctcc ataccacttt ccctgaacaa cttgaagaaa
     8221 tcagtgatac ttacgagaaa ttgatggaag aacaatatgt attcccagaa gaagatcttg
     8281 ctgaagatat cgcaaaagta agtcgaagaa ttgaaaattc attagccaat cttgaaaaaa
     8341 cagaagttga aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt gattcgttat
     8401 atgatatctt agaacgggaa atggaagctc agagatatgt aaaaactaac caatcaacga
     8461 ttgcagagta tatcaaacat acaacaaaga ataaccgtca attattgatt gagctggatc
     8521 acacatctca aagctataca ttgaaccaca atgaaattgg ccgtgtacga ggtttccaaa
     8581 ccgaagtgga agaaatggaa cgtcaaaatg aacagatgat tccacagatc cgtcaacacg
     8641 aaattcctta ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc ttagaagata
     8701 tcgaaaagca acaagtagag atcgacgact cattacatga attgagaaaa ggcgaaaaag
     8761 aagcgcaaga aaagatcgat acattcgaat tcaaattgcg cagtttgaaa cgttttgtag
     8821 aaaaacaacg tttgcctggc ttgccgaatg attacctaga attctttttt gttgctactg
     8881 accgtattga agaactaagt gttgtcttga ataaaattcg agtaaacatg gaagaggtca
     8941 atcgtttagt tgctctatgt gaagaagatc tagaattatt agataagaag acacatgatt
     9001 tagtagatgc agcagcatta acggagcaaa tgctccaata tgcaaatcgt tatcgtcata
     9061 cacacgaaga agtgagagaa gcaattgata aaagctacta tttattcaat aaagaatatc
     9121 actatcaaga agcattagac gaaatcggta cagcattaga gcgagtagaa cctggtgctt
     9181 tcaaacgcat cgaagatttt tattttaatc atccagactt agtataaagc aaaaaagtta
     9241 gtaaaccagc aagagcaaga cggattattg tcttgttctt gtcttttttt gtatatttga
     9301 aaattgaaac aagtgaaaat ttcacctgct cttgaaagca ccttggacaa ccgttataat
     9361 agaaaagaat ttcaaaaaag aggggtttct tatgatatat tttgataaca gtgcaacaac
     9421 cccaatcttt ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga ttattgggaa
     9481 tccatcaagc ttgcatgatt taggcaatca agctaaccgg ttgttgcagc aagcaagaaa
     9541 gcagatcgct gatttgatcc atgtagaacc agaagaaatc tattttactt caggcggaac
     9601 agaaggagat aactgggtat taaaaggaac gatgatcgaa aaaagagaat atgggaatca
     9661 tatgatcatt tcaggagtcg aacatccggc cgtcagcgag acagcagaac aattaaaaga
     9721 attaggaatc gaagtaagta tcgccccagt ggataaacgc ggatttgtac gtgtcgacga
     9781 attgaaagaa ttaataagaa aagagacagt attagtctct gttatggctg tgaataatga
     9841 agtaggatcg attcagccca tccaggaaat cagtgatctt ttggatgatt ttcctaaaat
     9901 tcatttccat gtagatgctg ttcaggcgat tggaaaagta ccattctcgc aatggttgac
     9961 agaaagagtt gattttgcaa cattttcagc tcataaattc catggcccac ggggaactgg
    10021 tttcatttat tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg gagggcaaga
    10081 aagaaatcag cgaagtggga cagaaaatgt tccaggtatt gtggctatgg ttaaagcttt
    10141 acgtttatca ttagagataa aggaacaaaa acctgagcag acacaacgct taaaagtagc
    10201 tctaatcaat gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag gaactgacta
    10261 tgcaccgcat attttatgtt ttgctctaaa aggaattcgc ggcgaagttc tcgtccatgc
    10321 cttagaagaa aaacaaatat ttgtttctac tactagtgcc tgctcctcac gtaaacatat
    10381 ggcaagcagt acattgcacg caatgcatgt accaaatgat ctggctacat ctgctgttcg
    10441 aatcagctta gatgaaaaca attcgcttgc agaagtcgag caatttatga ttattttcaa
    10501 gcagctttat aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg tgaaatacag
    10561 tgaaattatg gtccgttatg gggaactttc gacaaaagga aagaatcgaa aaagctttat
    10621 catgcaacta gcacaaaatg tcagacaagc tttgaaagac tttccagaaa tcaaaatcca
    10681 tgctgatcgg gaccggatgc atcttttatt gaatggcgca gacgggcagc tagttattcc
    10741 aaaactagca aaaatatttg ggatccaaaa tttctctcca agtattcgtg tagaaaaaga
    10801 tgtcgaagtt ttgaaacaag ctgttcaaga catcatgaaa gaaatttata ctgggaaaga
    10861 aacatttaaa attatcgcta aaagaagcga tcaccaattt gaattgacaa gtaatgaatt
    10921 gaatcaaaca ttaggtaatg ctgtttttga tattttcccg catatccaag ttcaaatgcg
    10981 tcaaccagat attccattac gtgtagagat tcggcgtgat ggcgcttatc tatcgtatga
    11041 aacgatcaag ggagctggag gattgcccgt aggaacaagc ggaaaaggaa tgcttatgct
    11101 ttctgggggg attgattctc ctgtagcagg gtactttgcc atgaagcggg gagtggaaat
    11161 tgaagctgtt cattttgcta gtccgccgta tacaagtgaa caagcactgc aaaaagcaaa
    11221 agatctaaca gccaaactag caccttatgt gggaacgatc cattttatcg aagtgccatt
    11281 taccgagatt caagaagaaa tcaaaaagtc agtgccacaa ggatattgga tgacaattac
    11341 tcgtcgaatg atgttgcgtt tgaccgatga aattcgccgt attcgtcatg gtttagtcat
    11401 tttgaatggt gaatcattag gtcaagtggc ttcacaaact ttgcaaagta tggtggctat
    11461 caatgaagta acgaatacgc caatcattcg gcctgtagcg acaatggata aacttgaaat
    11521 cattgaagta gcagaacaga tcgatacatt taatctttct atccagcctt ttgaagattg
    11581 ctgtacgatt tttgctcctc cacagccaaa aacacgccct caactggaaa aggtccatca
    11641 atatgaagag cgtttagcaa tagatgaaat gatccaacga gcactagctg gcttgaagat
    11701 ggaagagata caagcagaat cgtctaatga aaaaactgaa gaattttcag aattgctata
    11761 agaggttatg aaaatagtgt tttgaccaga gtattagaga aacaaaacga aaaaatagct
    11821 ccatgtattt taccgttttt gaactaatgt cgaagattag ctgattgaat accatttatc
    11881 aagaagttat ggaaaagccg gttctttttt actaactgaa aaataaaatt ttatttagag
    11941 atcaagctac agttgataaa tgatataaaa aagagaattt cccatgaaat agcgaggttg
    12001 tgacagaagt gggcagcttc aagaaataag aaggaattta cgaaaattgc ttttcaaatt
    12061 tttgtgaaat ttcggcttat ttttcgaaga agttacttct ggaacaccgt gtatcagagg
    12121 ttg
//
LOCUS       DAYYAR010000089        11945 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000089 DAYYAR010000000
VERSION     DAYYAR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.089"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..235)
                     /locus_tag="ACHAT0_002010"
     CDS             complement(<1..235)
                     /locus_tag="ACHAT0_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8451939.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLL"
     gene            complement(241..1104)
                     /locus_tag="ACHAT0_002011"
     CDS             complement(241..1104)
                     /locus_tag="ACHAT0_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8451940.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(1118..2014)
                     /locus_tag="ACHAT0_002012"
     CDS             complement(1118..2014)
                     /locus_tag="ACHAT0_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8451941.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(2189..3478)
                     /locus_tag="ACHAT0_002013"
     CDS             complement(2189..3478)
                     /locus_tag="ACHAT0_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8451942.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(3450..4967)
                     /locus_tag="ACHAT0_002014"
     CDS             complement(3450..4967)
                     /locus_tag="ACHAT0_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8451943.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5165..6055
                     /locus_tag="ACHAT0_002015"
     CDS             5165..6055
                     /locus_tag="ACHAT0_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8451944.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(6128..6952)
                     /gene="nadE"
                     /locus_tag="ACHAT0_002016"
     CDS             complement(6128..6952)
                     /gene="nadE"
                     /locus_tag="ACHAT0_002016"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8451945.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(6964..8436)
                     /locus_tag="ACHAT0_002017"
     CDS             complement(6964..8436)
                     /locus_tag="ACHAT0_002017"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8451946.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(9073..9435)
                     /locus_tag="ACHAT0_002018"
     CDS             complement(9073..9435)
                     /locus_tag="ACHAT0_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8451947.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            9658..10410
                     /locus_tag="ACHAT0_002019"
     CDS             9658..10410
                     /locus_tag="ACHAT0_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8451948.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(10550..11473)
                     /locus_tag="ACHAT0_002020"
     CDS             complement(10550..11473)
                     /locus_tag="ACHAT0_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8451949.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(11488..11760)
                     /locus_tag="ACHAT0_002021"
     CDS             complement(11488..11760)
                     /locus_tag="ACHAT0_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451950.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         3740 a         2416 c         1955 g         3834 t
ORIGIN      
        1 ttaacagact aaggaacaag ttcttaccaa aatgttgctt tattccttga aagaaaaaat
       61 aaatgatatt tcctgatccc tcacctttcc attgcgccat tacataaaaa acagcacatg
      121 tagcaggcac gatagtaata acaggcaaac acacgattac ccagagaata cctaataaga
      181 tgtaatcaga aaccacaatc aactgtccat aaaatttagt ctcaactatt ttcatcttcc
      241 tcactctttc attccagaca tagctgttcc ttgaacaaaa tatttctgtg caaaaatata
      301 aaccactagt aatggcagca ttgaaataac cgttccagcc attacaggtc cataaaaaga
      361 aatgttctgc cctgtaaata aagctaaccc tgctggtaaa gttctcattt ttgaactact
      421 tgtcaatatc aatggataaa gcaagtcatt ccaactattc atcagcgtga aaatccctaa
      481 tgataaaaga gtaggtttgc tcaatggcaa catgattttc aaaaaaatat taaactcact
      541 taatccatca atgcgtgctg cctcttccaa agaatctggt aaagtaataa aaaaggaacg
      601 catcatgaag ataccaaatg cagttgtcat acgaggaatg atcaaacctg cataagtatc
      661 aagcatgcca agcaaattga cttcaataaa tagaggaatc atgaagactt ggaatgggat
      721 catcattgtc agcaaaacga aatagaacaa aactgatttt cctttgaaac gcatacgagc
      781 aaatgcatac cccgcaagag aatcaaagaa tacagaagtg atcactgtcc ctccagcaaa
      841 aatcactgta tttttgatgt aatctaataa agggatcgtt gtccaaacct taatatagtt
      901 atctagggta tagctatctg caaatattct aggcggaaat gagataatgt ctttttcgtg
      961 tttaaaagaa gaaataatca gccacaaaaa aggaaacgca ataaccaaca atataactag
     1021 catactaatc gttcgaatga tttttttacc actaactgct tttcgactat tcacagtaac
     1081 tgtaatcgag ctttctggtg ccatacaggt ttccctccta tttcatttgt ttttctttcc
     1141 gtgacatgta cacattagtg gagatagcta tagttgcaac aatgataaac aaaaccgaag
     1201 acagtgctga tgcatagccc aattcataag gtgctgtaaa tcctcgactg taaatatatt
     1261 gaacagctgt ttctgtctta aattgtgggc caccagcggt tgcaacatat acttggtcaa
     1321 acacttgcat cgaaccaatc agattcgtta aaatacaaaa acctaaggag ggaacgatct
     1381 gaggtagcgt aatgttaaaa aactgctgtc gatttgttgc accatccatt tgagcagatt
     1441 catacaaaga gggagagatt ccctgaatag cagtcaagag aatcgtcata gtaacaccaa
     1501 aattcttcca aacagtcatg atggctaccg tcggcatagc aagggttgga tcgcgaaaaa
     1561 attgagggat cggcaagccg acttgtgaaa acaagtaagg aatcgcccct acattcggat
     1621 caagcaacat agaaaaaata atcgcaattg acgtcaatga acaaataact ggaatgtaaa
     1681 atgtcgaacg acaaaaacga ttgaatctgg tatttttaga taatgctact gctgctaata
     1741 aaccaacgat aatttgtgtt ggcgtttcga gtacggtgaa atataaagaa tgaatcaacg
     1801 aattaactgc tcgttcatca ctaaatatac gtgtgaaatt ttcaaaactt acaaaatttc
     1861 ttgatgtaaa gaaaatattc aaatctgtaa aactaatagc aaatgttcct aaaagcggta
     1921 tgacagtaaa taataataaa actaaaaaag atggagttaa aaacaaatac gcagcacgat
     1981 caggacgatt ccaatatttg gtgaatccgt tcatatagtc cacctcatct ttcattttga
     2041 taaaatatac aatcttggtt taaacgaatc tgaataagat atccgaacaa tcgctgtgat
     2101 ttttgcaata acacctatct gcaaatacta ggtcgatcat tcagatatct gtatcttgcc
     2161 aacagtctat cctgtataaa gttctgtact atttttgcag taattgattg atggcatcat
     2221 ctgcatcgtt catgagtgtc tcaggatctt ctcctgctaa taatttttca atcagtggat
     2281 tgatgatatc gttattgatc gcagagattt gggtaaaacc tggtaaatat ggctctgcat
     2341 agtccatttg tttcgataat tcagatacaa taggatcatt ttttacttct tcatcttttg
     2401 ctactgaatc caagtaggca ggaaaaccat tttcgatact ccatttttta ccaatttcag
     2461 ttgtattcca atatttcaaa aactcataga cagcttcttt tttattatta tctgtacttg
     2521 ccggaatggc atatcctacg ccatctaaaa tagctgtttt tttgtctgaa tcactccctt
     2581 gtggtaaggt agtcacatta taatcaatct cgtttgcttt caatccgtta tttaaccatg
     2641 gcccattgat ttcaatggct agttgaccag cattcattaa attgtccatt tcggcgccag
     2701 aaatcgattc tggaccagtc ttgtctacat ggatcatttt ttggatcgct tttaacgttt
     2761 taaggttctc aggagacgca aattcagact tagtataatc atcgttcact aatttaccgc
     2821 catttgctaa catccagtta tataacggtg cagttccatc cttattgaag acaaaacctg
     2881 atacattttt actactatca gttaattttg gtgccatttc tgtcaattct tcccaagtag
     2941 taggtggttg ttcagtatct aatccagctt gtttaaacaa atcattattc caatacatat
     3001 acatgccttg tacctgcatg gggacaaaat actgtttacc ttctacttca cctaattcaa
     3061 tagccgtttc tttaaaatca gatttatcta ctccatcata ttcccagaaa tcgtctaatg
     3121 gttgtaccgc tccgttttct acatactgtg caaaatctgg ataatttaat gcaataaagt
     3181 caggtgcttt tttagaagaa atcgctgtag gaagtttttc attcaaattg gcccaagtca
     3241 tgatatccat ttcaattttg atgtttttat catttgtttt attaaattca ttaacgattt
     3301 cttgcaatat ttcaccatca gatgcagtaa acccattcca aaaagtcaaa gtcacttctt
     3361 tatcagaatc agattctgct gaacttccgc caccgcaagc tcctaaaata cctgttgcag
     3421 ctaccaacaa agagccaatt aaccattttt tacctttcat tttttgttcc tccttcatct
     3481 gataaacgac atctgaccac attccaagat agcggtttca ataaacaatt gattttccca
     3541 tcttcagctt ttacatttgt tgaatgactt ggttttactt gttcactttt aggaagatta
     3601 gttgcttttg tatcaaatcc actcatttca gaaaaatcaa tcactccctc taatgataat
     3661 tcagacaagt gaacagttag attcatttct tcttctccac ggttaactgc aaatatgaca
     3721 agttctttgt catttattac ggctatactt tctaaatacg gcacttcttc aaaatcttcc
     3781 gtcgcatagt ttgctgaacg aatttgtggg cttaacacct ctccttgtcc atacacagca
     3841 gcttgcataa atggataata gatcgtctgt ctccaagctt ctccgtttgt ttctgtcctg
     3901 attggtgcaa taacgtttac caattgagca agacaagcaa tctttactct atctgcatgt
     3961 ttcaataaag tgattaatag acagcctaat agtaatgcat cttcaaaatt atagttatct
     4021 tctagtaaag gaggagcaat ctgccaaggc gctgcttgct catcttgttc cgctgaatga
     4081 taccaaatat tccattcatc aaaggacagg ttgatctgct tcttactatg tttcttcgcc
     4141 ttgactgtat cgcacatcgc tgtcacttca gaaatgaacc gatccatgtc taaggaacgt
     4201 gcaagataat gagaaagatc atttttttgg ttgccataat attgatgcag agacaaataa
     4261 tctatttgat cgtacgcttg atcaagaact gttaactccc aatcaccaaa tgtaggcatt
     4321 tgccgattcg aacttccgca taaaaccacc tcaattgaat catctacgag tttcatcgcc
     4381 ttagccgttt cctctgctaa ccgtccatat tcttccgctg ttttatgtcc aatctgccaa
     4441 ggaccgtcca tttcattccc taaacaccat gtcttaattc caaaagggct ctcttgacca
     4501 ttcgaacgcc ttaaatcact ccaataagtt cctttaggaa aattacaata ttcgactaaa
     4561 ttacgagctg catctactcc acgagtaccc agattgacag ccatattgat ttcggccccc
     4621 acatgctgac accagttata aaattcatga atcccaactt cattcgtttc aattgtccgc
     4681 caagctaaat ccaaccttct aggtctattt gctatgggcc caatactgtc ttcccaacga
     4741 aatcctgaca caaaatttcc accaggatat ctaatcatgg gaatgttcaa ttcttttacc
     4801 aagtgaatga cgtcttggcg aaatccactt ttattagctg atggatgatc tggctgataa
     4861 atcccttcat atactgctcg tcccatatgt tcaataaatg aactgtacaa ccgcggatca
     4921 actttgccta cacgcttgac actactaatc aacattgtgg cttccataac tgacctcctt
     4981 taatttatac atgaataata cacctcgttg aaagcgttgt caataatata aatgttaaac
     5041 ttacttattt tttatcgatt ttaattaacc aaaatattaa taatgattaa aacaaataat
     5101 ttaatataac ttttattaac aaatatgtta atatataaga aaaacctttt aagaggtgat
     5161 atatatgcaa ctacaaataa acgaagaatc cttaccggta tatgaagcat tagctagtaa
     5221 aacaaggatc aagatcatcc agctactttc caaaaaaaag atgaatgtca aagatttggc
     5281 aaaagaactt ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc tagaggaagc
     5341 aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc gtgtcgataa
     5401 aatcgatatt agttttccag aaaaaatatt caatgctttt gatacgaaag aaacatcgat
     5461 acctattggt cactatacaa attatgcaat tgaaccaaca tgcggtttag ctacgatcca
     5521 tgattttatc gggaaagtgg atgaaccacg ctattttatg gatcctcgaa ggatggatgc
     5581 ccgcattctc tggtttacca gcggatttgt cgagtatcag gcacctaatt ttttaaatac
     5641 agaggataca ttggagatgt tggaagtttc tgtggaaatt tcttctgaat ttcctttttc
     5701 aaatgacaat tggccttcag atatcacttt cagcctaaat ggtgtggagc taggaacatg
     5761 gacgagccct ggagattttg ctgatatccg cggtaaatat acgcctgatt ggtatccaga
     5821 taatcttaat caatacggct tgctgaagac gatccgaatt accaaacact tgacgaatat
     5881 gaatggagaa cccttgtcaa acatcacgat caacgacatc cctaaagagc aagacacttg
     5941 gcatttgcga atcgaagtga aagacgatgc caaacatgtg gggggctgta ctttatttgg
     6001 taaaggattc ggtaattacg atcaggatat caaattaaaa gtttactaca gctgatattt
     6061 ttcttttcta ttttataaaa caaaaacagc caatccctat gaagggacgg ctgtttttgc
     6121 tttattgtta tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt ttatgttgtg
     6181 tcttgttcca ccatgcttcg attttttctt gtgcttgagc gggaacttct tttccttcta
     6241 gataatcatc aatttcatta tatgtgacac ctagtgcgac ttcatctgca atcattggtt
     6301 tcccatcttc aagatctgca gtcggaattt ttgtgtataa tttttccggt gcattcaatg
     6361 cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa atgtcggctc
     6421 caccatcacc aaatttcgtg aagaatccag taatgttttc tgcagcatga tctgtcccta
     6481 atacggctcc tgctctttct ccagctacgg catattgcgt aatcatacgt tgacgtgctt
     6541 tgatatttcc tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat acttgtactt
     6601 gtgcatcaac agctggtttg atatttactc gcagactaac atcgggctgg ataaagtcaa
     6661 gagcagcttt tgcatcttct tcatctgctt gttcaccata aggaagacga acagcgataa
     6721 attgatagtt ttcatctttt gtttcttcac gcatttcttc catcgttagt tgcgccaaac
     6781 ggcctgctaa agtagaatct tggccgccgc tgatacctag tacaaaagtt ttaagaaacg
     6841 gatatttttt caaatatgct ttcataaagt caatactttt acgtatttct tcttttggat
     6901 caattgttgg tttgacacct aattcattga taatttctgt ctgcaggctc atttattctc
     6961 ctcctatgct tctgtatgaa gttctgctac gctttttttc attcgttcca tgatagccat
     7021 tttatggttc cagcattcag tagacagatc gactgggtat ttttgcggat tcaagtctcg
     7081 tttatactct tcccatagtg aatctagatt ttcttttgta tattctttga tttcgtttag
     7141 tgcaggtaat tcgtaaactc tttttccttc gataaagata tcttgtaaaa ctgggcgagc
     7201 tgtaaagtct cgaacagttt tattgataaa cgtatgcact ggatggaaca tgaagatagc
     7261 ttcttcttct cgagggtctt catcccataa tgtcacgtag tctccttcag atttcccatc
     7321 aaaatttcga gtgatgcgcc acacttgttt tttccctgga gtagttactt tttctgcatt
     7381 gcttgataac ttgatcgtat ccatcatttt tccttcatca tcttcgatcg atactagctt
     7441 gaatactgca ccaagggctg gctgatcata ggctgtgatc aattttgtcc caacacccca
     7501 tacatcaatt ttagcttttt gcatctttaa attcaaaatc gttgcttcat ccaagtcatt
     7561 cgaagcatag atttttgctt ccgtaaatcc ggctgcatct aattgttccc gaacacgctt
     7621 agatatatag gccatatctc cactgtcgat gcgtacgcct tggaagttga ttttgtcacc
     7681 taattctttc gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat cataagtgtc
     7741 gactaagaac acgcagtctt tatgggtttt agcgtaggcc ataaatgctt cataatcatt
     7801 tccataagat tggactaacg aatgagcatg ggtcccgcta gctggtatcc caaatatctt
     7861 cccagcacgt acattgcttg ttgcatctgc tccgccgata tatgccgctc tagttcccca
     7921 gactgctgca tccagttctt gtgcccgtct agttccaaat tccaatagtg gatcttctcc
     7981 gatgacagat ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat tgaccatatt
     8041 caacagtgct gtttctacta attggcagtg cgctaaaggc ccttccactt ggatcaatgg
     8101 ttcatttgca aaaactagtt ctccttcacg agcagaacga actgttgctt taaattcaaa
     8161 attttgtaaa tactccaaaa aaccttcagg atagacttct aatccacgca agtaagcaat
     8221 atcggaatca ttgaacgtca gattctctaa ataattgact aaccgttcca atcctgcaaa
     8281 aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa cagcatgtaa
     8341 atcagctcga cctaattccc agtatgtctg catcatattg atttgatata gatccgtatg
     8401 cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg tattactcct cttggcaagt
     8461 tttcatttcc tacccattat aacaaaaaaa attaatagga aaagaaatct tccatttttt
     8521 ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa tgaactccat ctgctaaaac
     8581 tatcctttct tattggtatt taacctaaac gagaaagaat acttatttta ttcaatgtat
     8641 ccgagacacg aagaaattag cgttttctat ttttcttact tgataaaata agtagatttt
     8701 cctactacaa tcgtagccgt taagataccg atgattgaac aaatataacc aaatgtctct
     8761 acaacataat caatggcaaa agtttttgaa gcaataatac gtctttcctt ttttgcttct
     8821 tatccccaaa gaaacggata taacttatta tgtattgaat ttttttggaa aggcgaatta
     8881 tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg tttttaaaaa
     8941 gatacaaagt aagaaagcag ctgtcaatag aatggaaaca aaaatggcga gacatcaatc
     9001 agtctcgcct tgttttttat tgtatacatt agatatggag caggttacca taaagatgat
     9061 tattagtcgt tattaaatag aatccttcgt atttttttgt tctatcacta caatatcttt
     9121 atttggacgt ttgaattgta ttccggcacg ttctagctcc tgtttcactc ctacatggac
     9181 tttgacatct tctcgtgtca tttttaaaac gtgatgaatc gcatagtcga tttcttgcct
     9241 tgtgattttt ttcttcgtat tgatcatcac gacttgcttt ttatcatatg tgtctaccaa
     9301 tagcagttgt gttcgatcta acgaaaataa tttgtaataa ttaatagccg tattcccaaa
     9361 aaagaattcc actactttag gataaaatgt cttaatattt aaattactat tcacaggtgt
     9421 ttcgattagc ttcatctcat tcctcttttc tatatagtcg atttagatcc aatcttaagt
     9481 tatgttacca tctttcctag cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa
     9541 ggatttacct tttatcactt aatagtataa cataatttca tgagaaaaaa atacaatttt
     9601 ttcttgacca tctcgttacg taaaggatta tgcttacatt tgtcaggagg aaaaataatg
     9661 gagtacacaa taaagaaaat gtcagagata tcaggagtca gtccgagaac cttgcgcttt
     9721 tatgatgaga ttggactgct aaaaccagct aggatcaatt cttcaggtta tcggatttat
     9781 ggcaaaaaag aagtcgatcg tctgcaacac atcttatttt atcgaacaat ggcattcaag
     9841 ttggatgata tccaagaggt cttagacaat ccatcttttg accaccagaa agctttaatc
     9901 aagcatcagc aaatgttgct ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc
     9961 caacagacac ttgatatgta cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa
    10021 gggtttaaac aacaaaagtt gaaagaaaat gaagaatcct atggacaaga gattcgacaa
    10081 aaatacggaa aaaaaaaggt tacagcatcc aatgaaaaat ggatgaatat ggataaagaa
    10141 acgtatgaag aaatgcaaac tgttgaaaag cagatgttat ttgatctcac tctttactta
    10201 aaagaaccag aagatgaaag cttagcagac cgtatcttcc aagctcataa gaagtggtta
    10261 tcttacagct ggcctgaata tcagccaaaa gcgcataaag gactaggaat gatgtacgaa
    10321 gccgatgaac ggttcactgc atattatgac gagcgaagtg gtcatggtgc agcaaaagct
    10381 ttaaatgaaa ttatccaaaa aaaggcgtaa tcttttacat gatccaacaa aaaagtacac
    10441 gtaaaatgac ctaataacaa aacagcagac acccaagtta gatttttatg ctaacttttg
    10501 gtgtctgctc cattctttca caagtttttt catcctactt taaatatatt catcgttgaa
    10561 taaacgtata aggtattttc actttatcct ttttttctgt aagaaacaat tgaaatgcca
    10621 tttctccgca agcttccaga tggtggtcga tggttgggaa accaagaagc tcgctgatca
    10681 gcaaattatc gcgtccaatc accactggtg tcttatcttg cagatagctt tgcaagatgc
    10741 ctgctgcaac catatccgaa tttgtaaaga caccatcaac tccaagctct ttaaaaaaga
    10801 agcctgcttg ttttcctttc tctgcatcta tacaatccca aaaaacaagt gattcatcaa
    10861 aagatggaaa aagttgatga catagctgca aagtgatttt tgagttataa cttagcctgc
    10921 cacttcgccc caacgtgatg cccaattgct ttataccttg ctgcttcaaa taagtaagac
    10981 cttctgtaat tgattgttct cgatcaataa agacacagct tgcctgtttt cccttgattt
    11041 cttcacaaaa aactagaggt ccatattttt gatatgccaa taaatcatct agtgtattgg
    11101 cacgagacgt aatgatcaat ccatcgaaag ctttagctgc aaattcttca agataccttc
    11161 gctctttttc tttctgatag cccgtcggta aaagtgtcac tttgtaatca taatcaaacg
    11221 ctgcttcaat gatcccactg atcaactgat caaaatacgg atgattggta taaggcagaa
    11281 tcactccaat gcttttcgtt tttccataac ttaactgtct ggcatttgca ttagggatat
    11341 aatcgacttc tttcatgacc gcaagtattt ctttcctttt ttcctcatct acatatggat
    11401 gtccattgat tacacgtgaa atagtagaaa ctgaataacc tgtgagcttt gcgatatctc
    11461 gaatgtttgc cactgctgct tcgccaccta tacaaaaaga ttttcttgta aatagtctac
    11521 cacagctgga ccgcattcaa aaaactgata catttttcct tcatgcgaaa aactgatccg
    11581 ctgcccgccc caatatatat cttttgtcaa attttcgatt tcattcattg gtatttcttt
    11641 tactatattc ctattcaacc cttgaaaact gtacaacgtt aatacggatc ccttgaactc
    11701 gacaaacgca taaaaataat tgttcgttgt gccatctgtt atctctacat aagtattcat
    11761 tgcaagccct ccttttgata ctttgagtat agtgtatgaa acgcgtctca tagcaaggag
    11821 tttgttttgc caaagaaaag ttttattcct ctttcattat taccaaagtc tcttatttta
    11881 aattgaatac attataatga tactgtaaat atatctttat tattagtgaa tggatattta
    11941 ctttt
//
LOCUS       DAYYAR010000090        11651 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000090 DAYYAR010000000
VERSION     DAYYAR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.090"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2564)
                     /locus_tag="ACHAT0_002022"
     CDS             complement(<1..2564)
                     /locus_tag="ACHAT0_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8451951.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIP"
     gene            complement(2786..3670)
                     /locus_tag="ACHAT0_002023"
     CDS             complement(2786..3670)
                     /locus_tag="ACHAT0_002023"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8451952.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3765..4202)
                     /locus_tag="ACHAT0_002024"
     CDS             complement(3765..4202)
                     /locus_tag="ACHAT0_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451953.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4419..5330)
                     /locus_tag="ACHAT0_002025"
     CDS             complement(4419..5330)
                     /locus_tag="ACHAT0_002025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8451954.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5707..6345)
                     /locus_tag="ACHAT0_002026"
     CDS             complement(5707..6345)
                     /locus_tag="ACHAT0_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8451955.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6349..8331)
                     /locus_tag="ACHAT0_002027"
                     /pseudo
     CDS             complement(6349..8331)
                     /locus_tag="ACHAT0_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            8511..9176
                     /locus_tag="ACHAT0_002028"
     CDS             8511..9176
                     /locus_tag="ACHAT0_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451956.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(9573..11606)
                     /gene="brxL"
                     /locus_tag="ACHAT0_002029"
     CDS             complement(9573..11606)
                     /gene="brxL"
                     /locus_tag="ACHAT0_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFM8451957.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT         3567 a         2385 c         1809 g         3890 t
ORIGIN      
        1 gggatagaaa gaacaaattt acctgtatca tcaatagttg tttcggacaa aacagcttga
       61 ttttttttca atgaaaccaa agctcctgct tcgcctgtac ctgtgattga gcgagaagct
      121 gcattgatca atgtggtact taatttagca ggctcaggag gaatcacatc gactgtcgtt
      181 ttggcagagt ccagttctgt cacttcttgg ccgcctctta cttttttagc agcaactatt
      241 tgcacttgat cccccgttgt tagtctttct tcgttgggta cttgaacctc aaacaaaccg
      301 ccttgttcgc cttctcccca cggattatat cctgtccctt cattttcatc tgtttgtcct
      361 tgtgtgagag cagtaatttc tacaggtgat ttcgtcgtat ctgcataagt aatacgtaaa
      421 gtcacttcta cttcgcctgt ccaagcatct cttggctttg cctcgacacc ttcgggaaca
      481 acgacatgcc catagatttt tttatctgca ttcgttggta ctcttaagga ttcaatcatc
      541 ggaagctggt tattggcact gattcgatta taatttttca ttccggcacc tgtctgagca
      601 aattcatttg ttaaacctgc actagggctt gatacaaaat tacctaagtc ggttccattg
      661 atcgataagt ctgtcatatc agaaaaataa tccggatctt tttcaaagtt atcgaacgtt
      721 actcctgcct ttcgccataa agcaatttct gaatgagcga tcttcaaaga agaatcgcca
      781 tttccttgaa atacccagcc acctatgcca ttagtctcgc ttccgatccg tttgttttgg
      841 aaatcgaaat aggtaggaga ttctaagtct acattcaaca catccccact gatgatgcca
      901 gtcttatctt ccccagtttt cccgaccatg actaaataag ttttttcgcc agccataaag
      961 ttcaaatcaa tatctccagt tgtatctacg gtagctccgg aatctgcgcg tatatcgata
     1021 tttgattttt ctccagtaac cgtaaaatca gctgaccctg gactttctag aaaaccagaa
     1081 gcgtcataaa attggatcgc ttggtttcct tgattgagtc tgttcacata agatttgcca
     1141 tcccctgcat ttcttacctt caatgagcct ccattagtta ctgttagttt ttgcgtaccg
     1201 ttctcaaaac gaactgcgct agaacgacca ctttgcttat caatatcgat gactgatccg
     1261 gctccttcta cgatcaaaga aagtcctttc cctacaaaac gcatcccatt gtttccaggt
     1321 tggtcgccat ctccagccat tttgaccgag atttgtccac cacttgatac atggatttga
     1381 ctagaatctc cttgcaggcg aaatccgcca gtagtcgctg tattgattgt gattttccca
     1441 tcattcgaca cagcgactct tgatcctgag cctctcattt ggataattcc gcgattgttg
     1501 tcgttggtaa tattgatctt tgtatttacc gaaaattcac ttcctgagcc actgacttta
     1561 aatgtgacat tcccgggtag cgtatcgaca gcctctcctt catcgctgac gccatctgcc
     1621 cgatcatagt caagagagac ctttgaacca ctcaagatat ggagtcctgg tgctgttttt
     1681 ccactgcccg tctcatcacc tgaatctaga aaaccataga attttcctag atatggtcca
     1741 taaagctgac taccattgat tacatttagc tgatagcttc tacaaaaata agctttaccc
     1801 ggagcggcat tcatcccttc tttcactggt tggacatcca atacaccgga aacaccatct
     1861 aatgtgacag ttgcacgagg ggcgtaaatc aggcctaatt tagaaccatc atataagtca
     1921 agtgtcccgg taaattttat ttctcccgta aaatgatatc tgtttcccca aaaatcggac
     1981 atataagcaa tgataatggc atttccatcg atatttggta tcaacccacc tggatgagct
     2041 gccgttcctt taaaattctg aaactcgatc gactgaggtg tctcattgat atagatgtga
     2101 tgtgtgccga aattatgagc aagtccgcca aaatcgataa cgatatctcg cttgatctcc
     2161 aattccctgt ctaaagtcaa aggctgagtt aaacgaatct tagctactga ttcgtctgcg
     2221 agaagctgcg tcagttcttc tgttgtggca gcttcgctgt acccatccgc tgaaacaact
     2281 ctaacctgta cctcttcact gctatcagac gtttgttcag ctacttctga ggtctcactt
     2341 gtcttatttt cttcttgcct tgtattagaa gtaggcgcct cactttggga ttctgtagtt
     2401 tcagaagtaa ttgattcttc tgaaaaagta gtcttgtcaa aactttgatt ctctgattgc
     2461 gttgattcgc ttgttttctc tacatcggca aaaacggatg tcaccatcaa gagagggaag
     2521 acaaacaaaa tcgacgcaat cagctttttt acatattgat tcattctcca ccatcctctc
     2581 tgacgttttg acaattttat tttaacgttt taatttattt gtaattgtac tttcatattt
     2641 aaaatatgta tttttatggc aaatttttct gttaggtgaa aatttctttt ttgtacaaaa
     2701 aaaggctgtg acaaaagtct tgtcacagtc ccttattccg aacaaccgat gtcctcctga
     2761 caactcttcg gtcacaacct ccattttaac aataaacctg taaatgtgag ggtaacactt
     2821 tgattgtcac aggtaattta tccccttcat ctccatcaac attcgtcgtc aattcttcct
     2881 catcagctaa tgacacaact aattgctcac aagtaagata ggctagattt ttcgtagatt
     2941 catctactcc ttttaatagg tctggcaatg ttttaagcgt atcgatcaaa gaagaatctt
     3001 ttaaataaac caaatggagc ttaccatcat ccacttttgc atttggaagt aatgtttcaa
     3061 aaccgcctac tgagttagtt agtcctatca gcaagatact gctctcaatt tcttccttct
     3121 ttccatcaac ttctacgtga aacgagtagg attgtgtgga agctaactgt ttgattcctg
     3181 acatgaaata agctagtttt ccaaatttcg tcttcttctc cgcatctaca tcattgattg
     3241 cttcagggat agagccaatt gcgacgacat tcataaaata gtcgtcattt atttttccaa
     3301 tatccaatgg cttgactgat tcaatcgaaa aatgattgat cgcttcctca ggttctagcg
     3361 gtatccctaa tgctcgagcc aagtcattca ccgtacccaa tgggaaaaat ccgaaatttg
     3421 gccgatgttc ttgttcagcg attccactga ttccttcgtt gactgttcca tctccgccca
     3481 tgacaaacac actatgatac ccttccgtag ccgcttcgcg agtaaagttt tttgcatccc
     3541 ctgctttttt tgtatgcaag acgaccactt catcaaacac cgattccaat tttgctatcg
     3601 ctaattgctc aaattccttt gcttgttcac cgcctgaact ggggtttacc actaataaaa
     3661 cttttttcat cactttcttt cccttctgat tgatttcttt aatataacga tagcataaag
     3721 aaaaaaatta ggctgtgatg aagtactgtt catgaaacat tcatttattt tttgacaaga
     3781 taaaataccc atactatgaa actgaggaaa attaaaacaa tgatatagga aataaaagaa
     3841 aatgataggc attgtatacc taataaagta aaaatcttag gtggcagata aaaacacaaa
     3901 aatagcaaga gaatgtaata gcgtttcttc cactttttgt tatcagacct tatagcgatc
     3961 agaggaatac caaatgcata actgaaaaac tcttgatgta ctgttaatat gtcacgacct
     4021 tgaataataa atgcaacaat ccccgctata attagtgtgc ctaataccaa aacggaaaat
     4081 tgatgaatat attttatata caaatgatcc attgtacttc ctgccttttt atttgtgaga
     4141 aaaacaaatc cgtatacgtg aataacttct agaaataaaa gaaatttgca aaaccactcc
     4201 atttatctac ttctacgtgc attttttaat atcgttttct ttatttaaga ttatacagaa
     4261 aaatatctag ggtggctgga ttctaagtaa aaaaaatttt taaaataaaa agaactaaat
     4321 caagtaataa ttaaactact gttgatttag ttctaataaa aagatttaga tgaacagttt
     4381 tattgattta aatagttcat attttttatt tataccgctt atttactact tgctgtacta
     4441 gaatctgaag tgaatgtact caaaacattt tcaaagtctt tgtctttgat ttttacatta
     4501 gcgtctttca atacttcgcc aattacttta ttctggaagg tactgtcact taattttgtg
     4561 tctgttgcaa tttctttcaa ttcttttttg tatttatcca tgtcgttgcc tttgttttgg
     4621 tttttcacca ttttaaccac ataatattcc gtagtataag ttgaagcatt ggtagaagtg
     4681 atcacatcag aaacttgacc atctttcaac ttaaaggctg cttctttgac ttctgccgga
     4741 accgttgtag tagtggaatc aaatttcact tttccgccat cttcttttgt cgtagtatca
     4801 gttgacttgt ctttggctaa tttagagaaa tcgtctccct tatcagcagc tttcttcaca
     4861 tcttttgcat catcttcact tgctacttgg ataatttgag cttccacttc tggatggaaa
     4921 gattcccaag ccgtttttaa atcttcatcg gtcaatttga tatgtttctt tagaccttct
     4981 tgaaaggcta gactttgttt gatactgtct ttgaatgttt gttctgtgta acctgcagat
     5041 ttcaattgtg aatcaaatga atcacccaat tgtttctttg tttgatcata ttgggaatcg
     5101 atttctttgg ttgatacgtc atctccatat ttatcttcaa atacttgata aacgatcaac
     5161 tgttgcacag cggaatctgc tccttgcatt tttttcaagc tatccattac ttcttgttcg
     5221 ctgattgtac cgcctttata actaacggct gttttttcac cggcattcgt acagcctgac
     5281 aataagccag tgcctagtac gatggcaagt gctaaaaatc tttttttcat ttatcaattc
     5341 tcctactttt cgttttttgc gaattgctaa aaacaaaggt gttcaagatg aaaatcatca
     5401 agaacacctt tactaatctg ctcgatacgt cctgaataga tataaggcta cacaatccag
     5461 aaagttcgct cagcagaata aatgtcaagt agcacatcac accttaatat actacttctt
     5521 ttgttaccgt ttttcaagtc tatcttgcgt tccgtattta acgtaactaa ttcttagaac
     5581 aaatgttatc gattttgttt ttcttcttcc gctgttggga tattcaaatc agacactttt
     5641 atgttcgatt ttccagacat ttttttgttg tttttattag aaaaagtttg ttttttcatt
     5701 tgttttctaa agcatgatct tcttgtttcc tattttggtc agatatggat catgcgtgac
     5761 cacgataact aacgtacctt gggaagcctg ttgcgataag atttcaacga cttgctcctt
     5821 atttcgttta tctaatgatc cagttggttc atcggctaaa atgattttgc tattcttcaa
     5881 taagactctc gcacaggcga ttctttgctt ctcaccacca gataatgtgt agataggcgt
     5941 acttagcttc ttgtttatat ttactttttt caatgcctca tactttgctg ttcgcttatc
     6001 tgaaacattt aaattcgaaa acttcaatcc aatatctaaa ttaaaatcga ttgtttcata
     6061 ttcaatcaac ccaaaatctt gaaaaagaaa agagagggca tttcttttta tcaagcgctt
     6121 ctctcttcca gttaaatgtt ttgtttcttg attaaataaa taaaattgcc ctgaactgaa
     6181 ttgatcattc aatcctaata tattcaataa agtacttttc cctgctccgc tctctccaac
     6241 tacagtcaca aaatccccag catccgcagt gaaacgatcc aaatgaagaa tcgttttgtt
     6301 tcgaatgatt tttttcgcac cttttatcac tatcaacggc tccaattgtt aacctccatt
     6361 catcagcaat aactgacttt tcttttctgt agacaaaata aaaacaactg aaagaataaa
     6421 atcaatgaag ctaaagaaaa aaatattaaa aagatattta ggctctcttt ctataagaaa
     6481 aaggaagatt acacttgtcg ttccccattt gatgaggttc atcatgaaga aatctttata
     6541 cgtttcaaag agcgaataac cacttgaacg aaagatagcg attttccgac aattgtagac
     6601 aaaatatatg cataacatga catagctcaa caagaagaaa gaaaagaaag tgagtaaaaa
     6661 tatctgtatc aagtaatgaa atgatctgct taacactttg acagctatct cttcagtctg
     6721 agtatacgga aatgataact gcaagttatc acgtaactga ctttcttcaa tcagctcaaa
     6781 taacctttct ttcgacaaag agcctatctt gatctttaac ggtggataga tttctccgct
     6841 gagaatattt ctttcccaat acgtactgtt tttcaatgtc agtatttcaa caaccggata
     6901 atgttcgatc caggggatag aaggaataaa tgtataaatg ggttgattgt tttttataaa
     6961 tagaaatttc ggttcttttt cactgatttc ttgataatat tctgttattt gcgccaggtc
     7021 attttttaaa tctgtatgtc tatatcgttc agggatcaat ataacagggt gcgtttcttt
     7081 ttcggttatt tggatcaacc ttccagagac atcaatcaaa ggaaatttct taagataatt
     7141 cagatttatt tttatccctc tgccaaactg ttcattttct tcattcaagt aatctgatac
     7201 attgactaat atacttccgt ttttattcaa agtttcatat aattcctgat cggctgctga
     7261 tgcaaaacga ttggaataaa taaaatccac gtggtttttc ccgatggtta ctggataaaa
     7321 tacatgatag tcctcaccag cgatttcttt tggtgtttca tgggaaacag ctaactgtga
     7381 agaagaaaaa acgatagttg ctaattcgac attggcaacg gtcaaaagaa gagaaaacaa
     7441 caatccaaat gtaaaaagaa tgtattggat atagttgaac gcttctctta acgaagcgat
     7501 tttttcaact aataaaagag aaatcaaaga gataccctat attccgctga acaagaaaac
     7561 gtacatctgg aacaaataaa gaaattgctc cttataatac ataaaaataa aaccaccaat
     7621 gattcctgca attatgaatg taataatgat tttctttttc cccaataaac accacaatac
     7681 ttgatgtaat gaaaagccat tattcaacaa taaactgatt tctttattca tcaataagaa
     7741 aaaggatgat aacaaaagga ataaaagaat caatccaata gttaaataca ctcgataatt
     7801 atatacgtct gtacttgaaa taaccgaata cgaatagcta ttttcaaagt cttcaaaaat
     7861 attagtggca taatcagtgt gaaatgcttg attgaatcgt tccacaaata gtaaaatgaa
     7921 ccgttcatag gcttcttttt ctctcgtcat caagaaaaat tcaccttcaa aatcttttat
     7981 tttgatcgat tcttcaatgg acgttacgcg aaaggaggta ttagaatagg gtaccttctc
     8041 gtgagaagat ttgacatccc cttgataata caatgtcatt tcaagctttg gctgtatctt
     8101 gaagttccaa gatgtatcta cttcattgga ttcatttact gtttttttca tataagaaag
     8161 ctcagtttct gatgagacat tttcaaatac catgattagc tgctcatatt cttcttgccc
     8221 aataaaatgt tgtggcaatc gaatcggagt ataagtatct gtttgatgga aaaattcatt
     8281 tgtccgatag agtacctgtt tatttataat aaccaaggca aaaccactca tgataccggt
     8341 cactatgatt gtgagtaaaa ctgtgaactt ttttttgatt atcaccactc tcccttactt
     8401 aaatgattct ttgataaaat cataccgttt ttcatcaaaa aaacgtatct atcaaatgta
     8461 taggtcatgt agaataaatc tatatactgt agaatcgaaa ggagaagaag atgaacatcg
     8521 gagaaaaaat acgaacttac cgcagaaatt gtaagcttac gcaaaaagaa ttaggcgaaa
     8581 aaatcggtgt ttccgataaa acaatctcta gttgggaaaa ttctcgtaca atgccagacc
     8641 ttgaaatgct gtcactttta catcagactt taggtctgcc gattgatttt ccttctattg
     8701 aatcagctac cgaatcaata gaaccaacat tgccggatga acctcttgtt atcaagaaat
     8761 cttattcatc aaaacaaccc ttctcactca aaaagacact atctttcttt ctgctcgtct
     8821 tttgtttggt ctatcttttt cttatcaatt atcaatataa aaatgaaacg gttgatcgat
     8881 tcaatccttt tttgaaacca caaatcggct ataccacact ccctgtagga tacgtggaaa
     8941 attccatcga acattgggta tgtaatgata tctttgttca tggtacatgg ctgtcttttc
     9001 acgctggaga ggtagaaaaa ggtaagaatc atgctgtaat caaacacaaa ggaaaacacg
     9061 tcatgaacgt tcgcctgctc acttgggaag ccattccctc agattaccaa gaaatcctca
     9121 tccaagacta tgacgatgcc ttaaaaagaa gccctcagga gccagatagg cattaagtag
     9181 gagatgaacg tagagataga aaaaatcaat gcacatgatc ttatcaaaaa aaaacagggg
     9241 tacaaattaa ttgaaaaagc tttgagatga aagttcctaa ataaaaaagt tcagaatgat
     9301 aggtaaaaaa atgactaact attctagtat taggctattt gtattattct tctgttattt
     9361 ttatatcaaa aaatccagaa ataactaaat ataggtattt ctggcttata gtataatatt
     9421 tacgccacag cccccaatga ttctttttgg aatcatgatc aaggacggac atacacaaaa
     9481 atattttgcc cattcgatcc gaatagatta acgactaatc tagtaagtaa tatagattgg
     9541 tacgtagtca aaccagcaaa tggaaaagtt gtctattcca ccccaagggc tttaaagaca
     9601 gcatcttccg gcgtttgata aaaaatcaaa ttaaaggtac ccattaattc tgggggtaca
     9661 gttccaatat cagcagcgct tatcataggt acaagtactt ttttcgctcc ggcatctttt
     9721 gctacttgta aacagttagc taaattatct accttaatca tagaaccacc aattgtaata
     9781 tcccctaaaa taactaaact tggtaataca ggtttattta aggaaactga gcataaagct
     9841 attatagttg ataacgataa tgtttttgtc ataccaatgc cattcatatc ttgaacatga
     9901 acgacaaaat cttttgtcgt caaactaata gactgactaa cacttccata attagctctt
     9961 aaatatcggt atgccgaatc catcgcttct cttgctccag catctgaatt gatcccagta
    10021 cgttcaaact ttccatttcc cgaagtcatt tgcgtttcca atttataggt acctaattgc
    10081 ccagttttgc cataagatac cgtgtacaaa tgccctggct tccctaaccc ttctggaatc
    10141 aaagttcctc caccttgttc aggaacagaa acatactctt ctcgctcttc ttctaaatca
    10201 atgtatgaaa aattgacatc ataaaattcc attccaccaa ttttctttaa ttgttctttt
    10261 acacgacgac gcatttcaag agcaaatctt agtatttctt cgacatcact tttttcaaat
    10321 ctaccatcag gatacaacag tttcatcatc ccagatacaa tccgacgaac tgcaatcgta
    10381 tcccgctgat ttaattgaga accaagtttg aaatatttat caaaactatc gccaaaacta
    10441 acttttcgaa gctcacgcat gacttcagaa aaataatctg taatgaagcc atagtcattt
    10501 gtaaaaaaat ctggtcgata tttaggaatt tcccaacctg gtaaataaca atgcatccga
    10561 tccaaaaagg ctgaatccgt ccccataacc tccggaaatg gctcaaataa attcgaagtt
    10621 ttcaatatta catcaacgct ttgattaata tttcctacaa aagccattga tgctgaagca
    10681 ttcttttctt ctttgcctct agcaaaagat cctgaagcca tgtaatcttt catgatttga
    10741 acgccatcat tatccttaaa tttaatccca gcaacttcgt caaaagcaac acagtcccac
    10801 atacctacaa gtcccattgt acgactagcc atattataga aaaggtttgc tactgttgtt
    10861 tgtccacctg aaacaaggat tgagtttggt gaaatttctt tatatacatg cgatttacct
    10921 gtaccacgtg gtcctagttc acagagatta aaattatttt caattaatgg aataagtctt
    10981 gctaaatgaa gccattttac ccttttatta aattgtgtag gttccatacc aatggaccgt
    11041 agaagtaagt caatccattc atttgtagta aactccttgc gagccatctt gtaagcctca
    11101 atgtctaaac caggcatttg gattggatca accttttttg caataaaagg gctattccaa
    11161 cgatcattct catcatacca atattccatc gaaacaatac accagatccc cccacctaat
    11221 aatcgatcaa attttttcgg atactcttca ctaatttgaa cattttttaa tcccaggtta
    11281 gagaattctg cttcataaca atctttcttt tcatttaaat gtacagttac tttatcaata
    11341 actgtataac cacccaattc acgaattttt gcttttactt tttctgcttc atcaggacgc
    11401 acatagtttc gagcaagaat atttttaaca ttttccacac ctttttcaac caatgtttca
    11461 tccaatgacg aagcatacat accaagtaaa tattccaata cataaacagg tacattcgca
    11521 ccttctttaa ttttctttgt taaatctttt ctgacaatct ttccgggaaa gaattcagat
    11581 aactttaaaa tttcctgttg ttccactaga attcctccat gtgttcagtc attataatag
    11641 caattaaagt a
//
LOCUS       DAYYAR010000091        11432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000091 DAYYAR010000000
VERSION     DAYYAR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.091"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..290)
                     /locus_tag="ACHAT0_002030"
     CDS             complement(66..290)
                     /locus_tag="ACHAT0_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8451958.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(488..2068)
                     /locus_tag="ACHAT0_002031"
     CDS             complement(488..2068)
                     /locus_tag="ACHAT0_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8451959.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2469..3845)
                     /locus_tag="ACHAT0_002032"
     CDS             complement(2469..3845)
                     /locus_tag="ACHAT0_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8451960.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3973..5061)
                     /locus_tag="ACHAT0_002033"
     CDS             complement(3973..5061)
                     /locus_tag="ACHAT0_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8451961.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5233..5667
                     /locus_tag="ACHAT0_002034"
     CDS             5233..5667
                     /locus_tag="ACHAT0_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8451962.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5745..6278)
                     /locus_tag="ACHAT0_002035"
     CDS             complement(5745..6278)
                     /locus_tag="ACHAT0_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8451963.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="ACHAT0_002036"
     CDS             complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="ACHAT0_002036"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8451964.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7543..8340)
                     /gene="recX"
                     /locus_tag="ACHAT0_002037"
     CDS             complement(7543..8340)
                     /gene="recX"
                     /locus_tag="ACHAT0_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8451965.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8447..9829
                     /gene="rlmD"
                     /locus_tag="ACHAT0_002038"
     CDS             8447..9829
                     /gene="rlmD"
                     /locus_tag="ACHAT0_002038"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8451966.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10003..10581)
                     /locus_tag="ACHAT0_002039"
     CDS             complement(10003..10581)
                     /locus_tag="ACHAT0_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8451967.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10903..11373
                     /locus_tag="ACHAT0_002040"
     CDS             10903..11373
                     /locus_tag="ACHAT0_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8451968.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         3364 a         2293 c         1949 g         3826 t
ORIGIN      
        1 aaatttttgt gaattccagc ttatttcttg gagttaaacg cttctgtctc agcctctttt
       61 tctttctagc tttttgattt ttctcttttc ttttgtactt ttctcttttc attttcagtt
      121 ctacgcgtgt ctttattttt aactttttcc tctttatgga tgattgatcg tactttttgt
      181 gcaatttgct cgtttttgtc ccagtctttt acatctaata atcgacataa tcgtaacatc
      241 tcttcttttt tctgtttttt tctttcacgt ctttgccatt ctgtttccat gtgtcacact
      301 ctcctttctt taatcatatg tacgcaaaat aatttttata tttttttgat tagacatttc
      361 tatgatttta atatagtaaa aagagccgtc acggaaaatc cgtcacggct ctaaacaaaa
      421 tatgagaggc tgtttttgtt tggctattct tattacttga tgcagggcag cttgtgtcaa
      481 gacttcttta catcaaactt tttaatttta cattgggata tttatccgca aaccagcgtt
      541 ctgcaaattg gttctcgaat aagaacaacg gttggtcgaa acggtcacga gccaagatat
      601 tgcgacttga actcatcttc tcatccaaat cttctggatc gatccagcga gcgattttgt
      661 gtcccattgg agacatgatt acttcggcgt tatactcatt taacatacga tattggaata
      721 cttcgaattg tagctgacca acagctccaa tgatatattc ttccgttaga tatgttttat
      781 aaagctgaat cgctccttct tgaactaatt gatagattcc tttgtggaag gatttttgtt
      841 tcatgacatt ttttgcagtc actttcataa acagctctgg agtaaatgaa gggagttctt
      901 cataagcaac ttttagtttt ccttcgaaca atgtatcccc aatctgataa ttgcctgtat
      961 catatacacc gatgatatcg cctgctactg ctttttcgac gttttctctc gaatctgcca
     1021 taaattgtgt gacattgctt aatttcagtt ttttatttgt acgttccaac cagacatcca
     1081 ttcctcgttc gaatgttcca gaacagattc ggacaaaagc gattcggtca cgatgggctg
     1141 gattcatatt tgcttggatc ttgaatacga aacctgaaaa ttcttcctca taaggactta
     1201 ccatctgatc atcttgtgtt ttgtgtccat aaggagctgg tgccaattca acgaaagtct
     1261 ctaaaaaagt ctgtacaccg aagttagtca atgcagaacc aaaaaatact ggtgtctgat
     1321 cgccacgagc aattttttct tgattaaatg catctccagc ctctttgatt agttctactt
     1381 cttctaagac ttgttcatat aaggaatttt cagttagagg atgattttcg ggaagcagtc
     1441 catcttcatc caattctact aatcgttcat cctgataatt ttccggacga taaaactcta
     1501 cacgattatg gtagatatca taaagacctt ctagcccttt acccataccg attggccaat
     1561 tcatcggata agattcgata tctaatagct cttctaattc ttctaacaat tccaatggtt
     1621 cacgaccatc acgatctagt ttgttgataa aagtaaatat cgggatgcca cgttttttta
     1681 caacttggaa taatttcttt gtctgtgctt cgatcccttt tgcactatcg attaccatga
     1741 ccgcactatc cacggccatc aaagtacgat aagtatcttc agaaaaatct tcatgtcctg
     1801 gtgtatccaa gatatttact cgtttccctt gataatcaaa ctgcataaca gaactagtaa
     1861 cagaaatccc acgttgtttc tcgatttcca tccagtctga tttggcaaaa ttacctgttt
     1921 ttttgccttt tacggtcccg gcttgacgga tagccccacc aaacaatagt aattgttctg
     1981 taatcgttgt tttcccagca tccggatggg aaatgatcgc aaaagttctg cgattgtcca
     2041 cttgttgctt taattttgga ttattcataa cctaactcca tttcttccat tcaattattt
     2101 atattttctc taggcacaga ttgtccgatc tcttaaagaa agacaatttc atatagggct
     2161 gtgacaaaag ccgtgtagca gtctcttttt ccgagtaaac ggcgttctag agacagccta
     2221 tcagcaataa gcccacccaa gccaaaaata cgaaaagctg tttttgcttg gctgctctta
     2281 ttacttaatg caggatagct ttttcacaac ctttgtcaac tactacaact gaatcagtat
     2341 agcatggatt cagcgaatga aaaagccacc aaaccataag agtcaaatat ttttttgtca
     2401 gctgactaaa agaaaagacg tgttgcttgt ttattcaagc aacacgtctt tcataaccaa
     2461 gtttccaatt atcttcttgg ctcgtcatct tcgaattctt ttctgaagta tcttcgttct
     2521 tcttcaggtt ccgcttctgt ttcttccgga tcatgaaaaa tgacacggat tttaaggatt
     2581 cgagaacctt ccatttcttc agaaaccagc gtaatattat cgacatcaaa cgataacttt
     2641 tcgccttcat ccggaatcat gcctaatgct gtaatcaaat aaccagccat cgtatctaca
     2701 tcactcatat gcagatcact gtcaaaagct tcattgaact catcaatcag catacgtcct
     2761 tggatgatat attcgtgttc atcgattttt tcataaagat tctcaacttc atctgattca
     2821 tcatcgattt ctccgacgat ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg
     2881 ccgccatatt catctaataa aatagccatc tgattctgcg tctttttcat ttcatataac
     2941 agatcatcaa taaagaccgt ttcaggaacg aataaaggct cctgcatgat tttcttgata
     3001 tctaaattat caaagccgaa tttatgggca gcttttagta aattctttgt atgaaggatt
     3061 ccgactactt tatctttgtc ttcgttataa acagggattc ttgaatagtt ttcggataaa
     3121 acttcattca cattttcttc cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt
     3181 gggaccatga cttctcgggc aactttcgta tcaagagaaa aaaccccttg gagcatttct
     3241 aattcttcat tattcaatac gccttcgttt tcaagcatat aacgcatttc atctctggtc
     3301 atttttgaat cttcatcatc aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa
     3361 gcagaaagca gccagacgaa gggcttagct actacaccaa tcaagcgtat cagccctgaa
     3421 gtgaactttg cgacttcttc tgatttattt aaagcaatac gtttagggta caattctcca
     3481 aaaacaatcg atacataggt caaaatagcc agcacaatga tatttgcaat attttttgcc
     3541 gctgtgccgc cacctaatac cggagctaac cgttccgata aagtattagc tagtgaagca
     3601 ccagacaaaa tgttgaccaa agtgattcct acttggattg tcgataggaa attatttgga
     3661 ttctgcaata tttttaaaag cttttgagat ttcacatctc cttcatcagc tttttgctcg
     3721 attcgattct tattgatcga aacaacagca atttcggaag ctgctaaaaa agcattgatc
     3781 aatgtcaaaa cgatcaataa taaaatctgc gctaacagcg actgactctc agggtcagca
     3841 ttcataatcg cattctctcc ttataaaatt aatttttagt ttacgataga aaaaaacgtg
     3901 gcactatagc cacgttaaaa tggtatcaca aagcaatttt ctttgcaata aaaacaagtg
     3961 tactaaaact acctacgagg tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc
     4021 tttgacgatt ttgattatat aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc
     4081 taagaagata ccaagcaatc cggcaatatt tccagctact agaagaacga taatgatcgt
     4141 taatggatgg attttcagtg atttcccaat tacattagga taaatgatat tcccatccaa
     4201 ctgttggacg attagcacaa ccacacaaca aagtaatgct tttaccggat cattgaagac
     4261 tgttactaga aatgcaggtg ctagtcctag ataaggtcct aaatatggaa ttaggtttgt
     4321 caatcccgca atgaccccaa ataagaaagc ataatccaca ccaattacca tatatcctaa
     4381 aaatgtgaag gtaccaacga ataaacattc gattgcttgt ccgctaatgt aattagccaa
     4441 tgttttattc agttgcccca ataactccac aatttggttt cttctttttt ctgggaaaaa
     4501 ctgttggatg tttggcacta gtttttctcc gtctttcaac atatagaaca agatgaatgg
     4561 tgctgtaatg attactacgg ttgccgaagc gatcgttcca acgatcgaac ctaaactatt
     4621 ggatactcca ctaaggaatt gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa
     4681 atatttattg atatctactt ccttgaacaa aggcaattcg gctacctctt ttaaccagct
     4741 ttctacctgt ttgatgaaat caggcatatt agatgccaat gaggaaattt gctgtactag
     4801 acttgggatg acactaagta aaatccaaat aattgctgca actaaaagga gcagcacaat
     4861 tgcgacagcc catattcttt tgatttttgc tttcatcaac agcttgacaa taggattcaa
     4921 taaatagtaa agaaagcctg caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc
     4981 gatcggttcg aaaataaagt ttatttctga tgaaatcatg atcaatgcag ctatgatcaa
     5041 taattcaagc gaccaaaaca ttagtttaga ctgtctaagt ttttcaaaca atagatttcc
     5101 tcctttcttt cttactagta tatattatat tcgttaaaaa ttcttttatt ctttttttat
     5161 ttttgaaaac ttgccatact ttttatttgt tttattttcc agtatactat acgaaaggaa
     5221 ggtggctaga ccatgaaagt tgtacaaaca aaagatacaa tgagcaatat ttacttagat
     5281 gctgtacgga tcaggcatca agtttttgta gtcgaacagg gtgtgccttt gtctcgagaa
     5341 atagacaaag atgaagccca ttgtatccac ttcgtcctgt actcagataa aaaagaaccg
     5401 caaggaactg tacgtctttt gcctttagaa aacggaaaaa tgaaattaca gcggatggcg
     5461 attctttctg aatacagaca ccaagggtta ggaaaaatat tgattgaaga agcagaaaac
     5521 tttgctaaga atcaaggcta taatacgatt ttattagggg cacagtccac agctgaaact
     5581 ttttacgaaa aactaggata tacagcttac ggcgatcctt ttgaagatgc tggcatgcct
     5641 catatttata tgaaaaagac tttataaaaa tttatttcga aaaaagcaaa aaacagcaag
     5701 cttttgagaa aaatctctca ctggcttact gttctttttt gtgtttactt acgcgacaat
     5761 tgcttgtaac ggttatacca aatatcaata tagccttccg agaacgggcc atctccatta
     5821 ttgatccagt caaccagaat cttcacattt tcttttagga tgaagtcaat atcgttcggg
     5881 taattcatca ttttgctgtg catctcatat tcatcgacat ccaatagtcg tttctcgccg
     5941 tcaggaaata ccttgatatc caagtcatag tcgatatatt tcaaggcctc atcgtccagc
     6001 aaaaaaggtg aagctagatt acaataatag gaaaccccct tttctctgat cattgctatt
     6061 acattgaacc agtatttttt atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg
     6121 cgtccatcag attcagtgac taatgtgtga tcattcacgc cgatcataga gtgttcgctt
     6181 gtttttaata ccatggtatc tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt
     6241 tggatcgtca caaactctcc ttcttttgga actcccatag caacccttcc ttaagcagca
     6301 ttttgtgctt tgtgtttggg tgttcattac accgacatca taattattat atcaataaaa
     6361 atgagctttg cctagaaagg attcctattg tttcgatttt tctctaactg atctaccagt
     6421 ttcatttgga ccttaggaaa aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa
     6481 gttttgttct cggtaaattc ttgttctgct ccctcggttg cccgtccgta aaaaaggaga
     6541 acatgccatt tcagatgact aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa
     6601 acaaagagtt cctgcttttc aaatgggaga ttttgctctt cttctgctac taggtcatca
     6661 aacagatcga tttctggctt tgtttcccat tcttttttca atcgctcata ctcctcttgt
     6721 gtcacttcca tcattggaaa tgtccacata ttcgccagca atttttggct atctctttct
     6781 tcaaaataat acgctccaga atgattttga agagctgcac tgatataata aacgtctttt
     6841 ggctttgctt tttttgtttt tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa
     6901 caaaaggctt ggatcggaca agtctcacac tttggagaag ttggtgtaca gatagctgaa
     6961 cctagatcca tcattgcttg gttgaattca cctggatgct tttcatcaat gattttccgc
     7021 atcgcttcat caaagatttt cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac
     7081 aatctgctga ctactcgcat cacgttgccg tcaactgcag gttctggaag tccaaatgcg
     7141 atacttgcaa tcgcgcctgt cgtatatggt cctattccct tcaatgagct aatttcttca
     7201 ggcgtttgcg gcatttcccc atcaaattct gacatgatct gtttagcagc agcttggatg
     7261 ttccgggctc ttgagtaata accaaggccc tcccatgctt ttaaaagctt ttcttctgga
     7321 gcatttgcta gctcttcaat tgtaggaaac cattccatga aacgataaaa ataatcgatc
     7381 actgtgtcta ctctggtttg ctgaagcatg atctcagaga tccagatacg ataaggatca
     7441 cgattatagc gccaaggcag gtttcttttt tcttgttcat accattgaat gaactgatct
     7501 tgaaattctt ttgtttcttt atctgtccat ttttcccatt ctttattcac tttcctcttc
     7561 cttttccgta atgaatcgct ggatcatatc caaatcaaat cctttttgat ataagctttg
     7621 ctttactttt agatttcttt ttttaggttc aaaccttaaa tttcttcgcc atagtttatc
     7681 tccttgtttg actaaataat catattctat ttcttgatct gcttcttgcg gaagatcagc
     7741 tatcgcttgt tggatcactt cttgagaaaa gcctttcgtc atcaatgttt gttggatttt
     7801 tctcaataat tctttttggc tttttccgtg attttttcca aacgcttttt cggctgtctg
     7861 atgtgcattt tgaacttggt cttcaaatgt atattggctg agcgcttgtt caatgatttc
     7921 aggtttcaaa cctttttgct gcatctgctg cgctaatttc tttggtcctt tgtcacttaa
     7981 gcgcatattc gtgcgcacat aactttcccc atagaccaaa tcatcgacaa ggtccaattc
     8041 ttttaagcgg gctacgattt tatttcgatc ttcttgaagg atttcctttt cttttaagta
     8101 tgttcggatt tctttttccg atcgcaactg ataacttaag taattcattg ctaggtgaaa
     8161 accgaaatca gctccgctac tcttcttgat ctcttggata gtttcttcgt ctaattcccg
     8221 tccttttaat aatcgatagc gtactaatac atcttcagat acttgtaaag cttctccttc
     8281 agataaatct acgcgataga aagggccttt gcctttactg attctcgtaa cggttaacat
     8341 aaacatcctc ctcgaacata tattctctat cataccacag aaaatccccg taatcaggta
     8401 ttatatgtta gaataaacca cagaaaaaag aaaaagggag atacttatgg cagaaacaat
     8461 tgtcaaaact ggacaaacgg ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg
     8521 aatcggttat ttcaaacggt tgatcatatt tgttccatat gcactaccaa aagaagaagt
     8581 actagtgaaa atcacgaaag caactcctcg ctatgcagaa ggagaattaa taaaagtcaa
     8641 aaaaacaagc aaagatcgcg tagtggcacc atgtcctgtt tactatgaat gcggtggctg
     8701 tcaattacaa cacttagctt atcaagctca attggatttt aaaaaggatc tgcttttgca
     8761 agcattggaa aaattcaaac cagcaggctt tcgatcttat caacttttgc cgacgatcgg
     8821 gatggatgag ccttggcatt atagaaacaa agcacaattt cagttacgaa aaaataagca
     8881 gactcaaaaa attgaagctg gtttatacga agcaaactct catcagttag tccctataac
     8941 agactgcttg gttcaagaac ctcgaacaca aaaagtaatg aatactgttg ttcaactatt
     9001 gaataaattt gatgcaccca tctatgatga acggacaaac agtggaatct tccgcacaat
     9061 catggttaga atcggtgtta aaactgggga gttacaagtt gtttttatta ctcgcagtaa
     9121 aaaattcccg caaaaaaacg caatgatccg tgagatcaat gaacgactgc ccgaagttgt
     9181 ttcaatcatg caaaatgtcc aggcacaaaa aacgtctctc gtgatggggg atgaaacgat
     9241 ccacttatgg ggaaaagaag ccattgaaga acaaatcaat gaagtgactt tcgatctttc
     9301 tcctcgtgcc ttctttcaac tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat
     9361 caaagcatta gatgtccagc ctaatgaaac gatcgtggat gcttattgcg gagttgggac
     9421 gatcggctta agtgtagcaa aacaagccaa agaagtacgg ggaatggata ccgttcctca
     9481 agcaatcgct gatgcaaaaa aaaatgcgga aagattagga tatcacaaca ctcgatatga
     9541 agtcgggaca gcagagcaag ttctgcctaa atggttgaat gaaggttttc agcctgatgg
     9601 tatcatcgta gatccaccac gtacgggttt ggataacgca ttgatccaat cattattgaa
     9661 ataccctcca aaaaaacttg tttatatctc ctgtaatgtt tctacacttg ccagagatct
     9721 ggttaattta agtaaaaagt tcaagattga atatttacag tccgtcgaca tgtttcctca
     9781 gactgcacgt tgtgaagttg tcgtgaagat gacgagactc gctgactaaa tgaaagaggt
     9841 tgtgaaaaag tctgccctgt atcaagtaat aagaacagcc aaacaaaaat agctcttcat
     9901 gttttatgaa tactgtactt gtgaccgaag agttaacagg aggacactat ttatttaaaa
     9961 aaagggttgt gacatagttt ttgtcacagc cctttttgat tattattgga tattcacgtc
    10021 tatcacttga taaaatgcat ttgtcgtatc gctgatattc catactgcta aaatcacatt
    10081 atatcctttt cgatcctgcg gaagtgtgat cgtttggttg actgtttttg gtggaacagt
    10141 tgctttgtcg tctatttttg tgatcaattc aaaatcacta aatttcaaag gttgattagg
    10201 attccaagtt ggtttggtaa tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa
    10261 attccaagta atatttaatg ttcctggatt aaccatgttt ttatgccatc tgttagatgt
    10321 ctgttcatct aaaggctcaa agccgctgac tcctgcacta gcaatttttc catcaatgaa
    10381 tgtatttttg gtagcttcga tactttgcgg ttcccattgt gctcttccga cattttgatt
    10441 taagtttcct gctgctgatg ttcctaggta tgcacggctt cctggagaag taatataacc
    10501 atgcgcaaaa cctggaatgc taaaaccgaa caaccctaaa ctaactaacc caataaacaa
    10561 taacgtacta atctttttca tgtagaaacc tccttcattt gataaccaca ttgtaaacgc
    10621 tttattgtat taacacaaac attatgcttt tataatttta tgttgaagta aaaaaggtga
    10681 aaaaagtaag atgcagatta taacagaagt agacgagaaa agtacaaata gaaaacatga
    10741 gaatcaaatc atatgatctt tttcaaaaac atcattcata taaacaaatg ttcaactttt
    10801 taataacaac aattatcaat ttttttctta cttcacaaac taaatacagg tcttgctgtg
    10861 agccttcatg gtaaactaat attatagaaa ggagttagta aaatgaagat gataaaagat
    10921 attcgttttt gggtttgtgt tataggaatt gtcatattag gctttttatc agggttgctt
    10981 agcggaaatc ccggaaaata ttactattct ttgcaacttc caccatttgc accacctagc
    11041 tggatatttg ggccgatgtg gaccttactg tatatattga tgggtatttc tctttatttg
    11101 ttacttaatc acaacaacaa aaaacagcga aacaatcttg tcggtctttt tgtgattcag
    11161 ttcattttta attttatatg gtctgctcta ttttttaatt taagaaatat tttcattgca
    11221 gcaatcgata ttacactttt agttattttc ttgtcagtgc ttatgtatca gttgtggctg
    11281 catcatcgcc tggcaatgtg gctcatgatt ccttattatt tatgggtact attcgcaact
    11341 ctattgaatt attcaattta ttttttaaac taagcgctaa aaagagacgt cctgtaattt
    11401 aacacataga aaaagtgcca cctaagttag at
//
LOCUS       DAYYAR010000092        11246 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000092 DAYYAR010000000
VERSION     DAYYAR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.092"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..669)
                     /locus_tag="ACHAT0_002041"
     CDS             complement(7..669)
                     /locus_tag="ACHAT0_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8451969.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(682..2184)
                     /locus_tag="ACHAT0_002042"
     CDS             complement(682..2184)
                     /locus_tag="ACHAT0_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8451970.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2732..4411
                     /locus_tag="ACHAT0_002043"
     CDS             2732..4411
                     /locus_tag="ACHAT0_002043"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8451971.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4538..5017
                     /locus_tag="ACHAT0_002044"
     CDS             4538..5017
                     /locus_tag="ACHAT0_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8451972.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5236..5916
                     /locus_tag="ACHAT0_002045"
     CDS             5236..5916
                     /locus_tag="ACHAT0_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8451973.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5924..7255
                     /locus_tag="ACHAT0_002046"
     CDS             5924..7255
                     /locus_tag="ACHAT0_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8451974.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7270..8232
                     /locus_tag="ACHAT0_002047"
     CDS             7270..8232
                     /locus_tag="ACHAT0_002047"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8451975.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8343..8681
                     /locus_tag="ACHAT0_002048"
     CDS             8343..8681
                     /locus_tag="ACHAT0_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8451976.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8760..9467
                     /locus_tag="ACHAT0_002049"
     CDS             8760..9467
                     /locus_tag="ACHAT0_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8451977.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(9570..11051)
                     /locus_tag="ACHAT0_002050"
     CDS             complement(9570..11051)
                     /locus_tag="ACHAT0_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8451978.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3556 a         1951 c         2349 g         3390 t
ORIGIN      
        1 tgttttttaa acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca
       61 gctttttgct acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc
      121 gatttcttgg aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg
      181 ctcatccgct acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg
      241 ctgctgacct cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac
      301 acgagccagt cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc
      361 cattgctgct atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa
      421 aataatcgag acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc
      481 ttgatagtat acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt
      541 cgtttttcca gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag
      601 ttctacatct ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt
      661 ccgtaacatc aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat
      721 ccctatagaa gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag
      781 tcccaattgt gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa
      841 tggattattc gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc
      901 tggcatttgt ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc
      961 aagtagctgc tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa
     1021 tgcaagaatc atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga
     1081 ttctcctaaa gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat
     1141 gatcagagta agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa
     1201 acttgccacg ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa
     1261 gctaaatgta tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc
     1321 tggatcagat agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag
     1381 ttcatttgca aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt
     1441 catacccata ctgccgcttg tttcgctgga ttcataaatt cctttgatcg tcatttcata
     1501 agtggtatct tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt
     1561 tgtttctgct agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat
     1621 tccttcacca tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc
     1681 tgacacacca ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc
     1741 agcaggttca ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt
     1801 gatggcagtt attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt
     1861 tacattatct aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa
     1921 actgcctgga tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt
     1981 tttaaatgaa gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc
     2041 ttgattcgct gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag
     2101 aatggctgtg aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt
     2161 ccatgcacgt ttcataaaat tcatattttg catccctcct agtagatgca tttgataata
     2221 gacaacctat cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt
     2281 tctgtatata taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta
     2341 tgtttcaaac cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac
     2401 agagggattt ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat
     2461 ttgttgtata ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt
     2521 cgcaaaagat ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca
     2581 gaacgcggat gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt
     2641 tgcatatgct tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta
     2701 aacttggatg tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg
     2761 cggcgttcgt gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat
     2821 tttagattgt gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc
     2881 tgattttacg tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg
     2941 acatgcagat gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt
     3001 cgggacagag ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa
     3061 aggatttaag gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt
     3121 agtgagcttt ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac
     3181 aaaagaaggt agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat
     3241 gtatcaaaca gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact
     3301 aagtgaatcg gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga
     3361 tgaagtattt gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag
     3421 taatttgcaa cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt
     3481 cttgactggt caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca
     3541 attaccaagt gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga
     3601 actgatcatt ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc
     3661 aaacggtaca caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac
     3721 gccaagcatc gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg
     3781 tggaacagtg aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga
     3841 cttgcaatta atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta
     3901 tcgtgaatta gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat
     3961 ctatattact ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg
     4021 aactacaagt gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat
     4081 tgttttacgt gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat
     4141 cagtcggcga gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta
     4201 cgtaaaagct agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga
     4261 gaaacattta gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca
     4321 attaggccgt tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat
     4381 ggaagctact caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata
     4441 gagtgctcgt cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc
     4501 gctttataat aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga
     4561 acgttattta gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga
     4621 agaatctttc acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac
     4681 tctcgtagtg aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca
     4741 cttagattac aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat
     4801 ggattttgta ttaatgcata caggatatcc ccacggagca aacaccccta ttggaataaa
     4861 actcgcacat cctgattatt gttttttagc agacagttct ttaaaagaaa aagaagaagt
     4921 aattgtgtca agcggtgaaa tcgggagaag ccttcggata aaagtagccg atttagaaaa
     4981 gatcgtccat cctatttacg ctgatttaat tagttaaggg acgagaagag aagctaagca
     5041 gagcagagac ctcaaacttg agattatatc tgcttggtac ttggtgcatc ctgtattatc
     5101 tgttacaatt gctttgtaca tactgtacta tcaatccaat gaaaatatat ttagcagcta
     5161 tattttttca ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa
     5221 cgcgaggtgt aaaatatgaa aattacgtat catggacatt cctgtttaac aattgaaatg
     5281 gaaaatggcc aacgcgtgat gattgacccg tttattacag gaaatccatt gacagactta
     5341 actgcagaag aagtaaagac agattggttg ctgattactc atggtcatag tgaccacata
     5401 ggagatatga ttcctattgc aaaagaaaat caagccacaa ttatcagtat tgttgagatt
     5461 gctgattatg cgaacagcag aggagtagat tcattcggta tgaatatcgg tggaaaacat
     5521 gcgtttccat tcggcgtggt aaaattcgta catgcacagc atagttcaag ctatgaagta
     5581 gacggtatcg ttcagtatat gggcgagcct agcggaatta ttattcaagc agaaggaaaa
     5641 acgatttatc atgcagggga tactgcttat ttcagtgatc taggattgct tgcagaagaa
     5701 ttcgatattg atgtcgcttt cttgccgatt ggcgataact atacgatggg gccagaagat
     5761 gcagcaaagg ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca ttacaatact
     5821 ttccctgtca ttgaacaaga tcccgaatct tttgtagagt tactggaaga tagtgaagga
     5881 aaaataatga gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga caaaacatga
     5941 tttgatttta gaatatattg aaagtttacc tgttggcaat cgaatttctg taagaagcat
     6001 cgcaaaagaa ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa
     6061 tgtcggacta gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa
     6121 aaaacatatt gaacgtttga catttggcga ggtcgtcagg atcattgaag gggacgtttt
     6181 aggcggggcg gcaggattag acaaggtctt gaataaattc gtcatcggcg cgatgactaa
     6241 acatgccatg gaacgctata ttacgccagg atcactaatg atcgtcggca accgggcgga
     6301 agtccaaaaa ttagcattgg aagatggcgc cgcagtattg atcacaggag gattcaatac
     6361 gactgctgag attgcaaagc tagctgatga tcttgaaatg ccgatcttac gcacctctta
     6421 tgatacattt acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa
     6481 agatatcatg cttgtcagtg atatctatat gccagctgaa aagactcatt atttgcagca
     6541 gacagatacg atttctgatt atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc
     6601 agtcgtcaac cgccatcatc gtttgatggg gattgttacg gcaaaagatg ttttagggaa
     6661 atcgccaaac cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac
     6721 aatgagtatt gcctctgtta gccatcaaat gatctgggat gggctagaag tcatgccggt
     6781 cgtttccgat gacttgacat tggaaggctt gattacccgg caagatgtaa tgaaagcgat
     6841 gcagcttgtc cagcgacaac cgcagattgc agatacgatt tcggatcaga tatctggaaa
     6901 tatccatacg attgatactg accgtgaagg aaatcgtttg gaaaacccta agttcaaatt
     6961 cgtagtagca ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg tacttagcga
     7021 aatcatttct gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat
     7081 tgaacaagtg aacctacatt atttgcggct gatccaattg gaaagcgaat tagacattcg
     7141 tccacgtgta ttggaaatcg gcagacgttc cgctaaactg gatatcgaag tgtttattga
     7201 aaacaccatc gtttccaaag caatcgttgt ttgtcaagta atggaacgtt cttagaaagc
     7261 aggagtgaaa tgacagtaca gcaagaaata gtagatacaa taaaaaaata cgataaaatc
     7321 attatccaca ggcatttgcg ccctgatcct gatgcattag gatcacaagt aggattagcc
     7381 gaactattac gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa
     7441 gggcttcgct ttttagcaga aatgcaagaa gttgaagatg tcttctacca aggagcgcta
     7501 gtcatcgtga ctgatacagc taattcaccc agaatcagtg atcaaagata tcagttggca
     7561 gaaaaactga tcaaaatcga ccatcatccc aatgatgacc cttatggaga tcttgtgtgg
     7621 gtaaatacaa aagcaagtag ctgtagtgag atgattgcag aatttgcttt gatgtttcct
     7681 gaatctttaa agatgaacgt taaggctgca cggttacttt atgcaggaat cgtcggagat
     7741 acagggcgct ttttgtatcc ctccacgacg tcccgtacat tagagattgc ttcgatattg
     7801 agaaattatc catttgatgc aagtgctttg aacagagaga ttgaacaaat gccgatgaaa
     7861 gtcgctaaac tttccggtta tctttatcaa aatatccagg tggatgaaaa tggtgcagga
     7921 agaataatcc ttccgcaaga aattcttgat aaatatggaa tcgatgattc agagacagct
     7981 tcgatcgttt cacttcctgg agtgattgac gaagtgctgg cttgggggat ttttgttcag
     8041 cagccttcag ggtattaccg cgtacgttta cgttctaagg ggccgatcat caatgaattg
     8101 gctaaacgcc atcatggagg aggccatcca ttagcaagtg gtgcgaatgc aaaagattta
     8161 gcagaagtag aggaaatcta taaagagatc caagatatat gccaatcgta taagagcaga
     8221 aaaacagaat gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc
     8281 ttttcaagat cttctttttc cgttacaata gtgctcacta aagattttaa aggagaaaga
     8341 agatgacatt accaaattgc ccagaatgtg gctcagagta cacatatgaa gatcgaggct
     8401 tgttgatttg cccagaatgc gggaacgaat ggaacaaaga agaacaagaa actgtcatag
     8461 aagacggatt agtggtatta gatgcaaatg gaaaccgtct gttagagggc gatagcgtga
     8521 cagtgattaa agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag
     8581 tcaagaacat ccgtttagta gaaggcgatc ataatatcga ttgcaaggta gaaggatttg
     8641 gaccgatgaa actgaaatcg gaatttgtga aaaagaatta aagaatattc aaggtctttt
     8701 cacattcttt tcctactact aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa
     8761 tgtatggtta tggaatgtat ggttttttag atccgacatt tcttttggtc attcttggat
     8821 tagttatttc cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag
     8881 tgagaagcaa aaacaatgtg actggtacac aagcagcaca atatatatta caaagtcaac
     8941 agataaataa tgtcggtgtt caacaaattg ccggagatct cacagataac tacaattcag
     9001 gtaataagat gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag
     9061 gtgtagcagc tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat gtcccattga
     9121 agattcgtgc agcacttgtt ccagcagcta actttggttc gatgatttcg ttccccttga
     9181 ttatggtagg tgtattgctt agctggaact ctacgttaat caatatcggg attttcgctt
     9241 tttctttagc attacttttc caattggtga cattaccagt cgaatttaac gcgtctcggc
     9301 gagcaatcca aattttgagt gaaggcggac tactgacgga agaagaagta ccgatggcaa
     9361 gacacgtatt atttgcagca gcactgactt atgttgcagc agcgctttcg acattcctgc
     9421 agctgttgcg cttgatctta ttgttcggcg gcaaccgaag agattaaaat aaagaagttg
     9481 tgaaatcagc gctttgatct gagctaatac cgagagttag ctgattgaat accatttatt
     9541 tggaataaga ggatgtgaca agaattttgt cacatcctct ttgttttgtt ctcataacct
     9601 tttttcatat acagttcatg aaccgctttt attaacttgc caaataacac tgtattgtgc
     9661 gttgtatcca gatagaaagt ctccgtttca tctgaaattt tctcatgctg atctgtaatg
     9721 atcaaatcag catgttctga atatgcggta attttaacag catctttgcg aaataactgc
     9781 tgcaaatggc tttgaagatg ataatttcct gtaaactgtt tggttatctg caaatggatg
     9841 agtaactgct ctttcgtttc tgtttgtagt aagtgttgga tcaattgact aaggtattga
     9901 ctgatttggt ttctttcccc attttttgaa aaaattgtat cgattttttc tcttaatttc
     9961 ttttcctgta taaaagaact agaatgattg gacgtggctt ctggaaagca aagtgtagaa
    10021 aaactactgc tggattcacc caacagttcg aaaaaaacaa ctgccaacac aagaaaataa
    10081 aaataaattg gtattttatt ttctaaatgg ttgagaggaa actgttgagt taagtaagtc
    10141 atcaattgac cgacttgtcg acaaataagg tttttgcttg tggaaaataa aaaaccaatc
    10201 tcctgttttt gtcgaaaaga gatttgattt ggaaaaaaac aatagaaaaa ataattaaag
    10261 taggctctct ctgtttctgt ctcttcacaa gggcgactca ctgaaaaaga agtaaagtat
    10321 tctgaataat catgtgtatg ctgcaataag tccatcaatt cagtgatttc agaagaagtt
    10381 tttggaaaat atgcctgttg tttgtttcgt aatatgaata aagttaacag caagtagata
    10441 tttttttctt gttttgaaat tttttctatt tgctcatctg tactgttcat ttttagtgca
    10501 tttaattcaa gtgaggaaaa tggccaatcg ttacctccaa atgtatctga aagaaaaaag
    10561 taaagataga gacggataga taactcattt ccttcaagag tacagcaatt ttttacagat
    10621 aggatccgta aatggaattt tattaaggaa tgattgagct gttttgtcag tttttcgaga
    10681 taggagggcg atatataaag ttgttcaagt aaatattttt ttggtacagc agtttgtgga
    10741 taattgatta gtaacatcaa atagttaaaa cgattggatt cttttaggta gcataatttt
    10801 aacttagaaa aaacagctaa ctttttagtt tgatcggttt ctttggtttg gatctctcct
    10861 ttatgattag aataaataaa ttcatcaatc aataaaaatt gcttgagtga tacatttatt
    10921 tttcggatca gttctttgat tgtccgtttt ggaatatgta agtcattgga taattcttgt
    10981 aaagaacaat tatttttttg aatgaccatt tgtagaacaa gtaactcttt ttttcttgct
    11041 tcaattagca taaatacctc ctttttattc tgtttttgta tatagttaga caaaaactgt
    11101 gagtggaaag tcatttcagt ctgtgttatt ctcacagagt gtcgccttct tttttatata
    11161 acgttattta atttttttgt taccttgttt attctattgt ttatttccta gggtattttg
    11221 tcaagatatt cttgttttta aggcgg
//
LOCUS       DAYYAR010000093        11180 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000093 DAYYAR010000000
VERSION     DAYYAR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.093"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHAT0_002051"
     CDS             6..305
                     /locus_tag="ACHAT0_002051"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8451979.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHAT0_002052"
     CDS             378..1739
                     /locus_tag="ACHAT0_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8451980.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHAT0_002053"
     CDS             1761..2021
                     /locus_tag="ACHAT0_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8451981.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHAT0_002054"
     CDS             2035..2787
                     /locus_tag="ACHAT0_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8451982.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHAT0_002055"
     CDS             complement(2881..3486)
                     /locus_tag="ACHAT0_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8451983.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHAT0_002056"
     CDS             complement(3539..4513)
                     /locus_tag="ACHAT0_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8451984.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHAT0_002057"
     CDS             complement(4891..5610)
                     /locus_tag="ACHAT0_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8451985.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHAT0_002058"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHAT0_002058"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8451986.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHAT0_002059"
     CDS             7161..8558
                     /locus_tag="ACHAT0_002059"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8451987.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHAT0_002060"
     CDS             8639..9079
                     /locus_tag="ACHAT0_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8451988.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHAT0_002061"
     CDS             9227..9553
                     /locus_tag="ACHAT0_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8451989.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHAT0_002062"
     CDS             9554..10303
                     /locus_tag="ACHAT0_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8451990.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHAT0_002063"
     CDS             complement(10383..10838)
                     /locus_tag="ACHAT0_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8451991.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3550 a         1958 c         2295 g         3377 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc
//
LOCUS       DAYYAR010000094        10391 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000094 DAYYAR010000000
VERSION     DAYYAR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.094"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(372..1958)
                     /locus_tag="ACHAT0_002064"
     CDS             complement(372..1958)
                     /locus_tag="ACHAT0_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8451992.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            2209..2433
                     /locus_tag="ACHAT0_002065"
     CDS             2209..2433
                     /locus_tag="ACHAT0_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8451993.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2575..4326
                     /locus_tag="ACHAT0_002066"
     CDS             2575..4326
                     /locus_tag="ACHAT0_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8451994.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            4326..6110
                     /locus_tag="ACHAT0_002067"
     CDS             4326..6110
                     /locus_tag="ACHAT0_002067"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8451995.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            6568..7419
                     /gene="licT"
                     /locus_tag="ACHAT0_002068"
     CDS             6568..7419
                     /gene="licT"
                     /locus_tag="ACHAT0_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8451996.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            7516..9402
                     /locus_tag="ACHAT0_002069"
     CDS             7516..9402
                     /locus_tag="ACHAT0_002069"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8451997.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(9443..10345)
                     /locus_tag="ACHAT0_002070"
     CDS             complement(9443..10345)
                     /locus_tag="ACHAT0_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8451998.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
BASE COUNT         3273 a         1866 c         2189 g         3063 t
ORIGIN      
        1 ttgattttgt cgctttcgtt tatgagctat ttttcttaca ccttttactt gactaatcgt
       61 aggaaaggga actgtgacaa aagtcttgtc gcagttcctt tttccgaata aacggtgctc
      121 aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacataaaaa gctgttttta
      181 cttggctgtt cttattactt gatgcaaggc agctttttca caacctctga tacacggtgt
      241 tccagaagta acttcttcgg aaataagccg aaatttcaca aaaatttgaa aagcaatttt
      301 cttaaattcc ttcttatttc ttgaagctgc ccacttctga cacaacctcc tttgtgtaaa
      361 agaatcagtt tctactcgat ttcttctatc gtttcctgca aacgctgttc atattttttg
      421 tttcctttat ataaatcacc aatcgtccat gccaacagga gaaggacaag aacaatgatt
      481 acataagcga tttgatcagc caatgctagt tgagctgagg tggcttgctc gccgaagaaa
      541 ctttcgattt gacctggtaa tcctcgtaaa ttcaagtaag tcagactaat cacagagaac
      601 cagccaagta gttttacaac aaatgaattt ttgaatcggc tccccatctc cgcttggcta
      661 ttggtcatca tcagtaaagg aatcatcgag aatggcaatg caaaagccag aaaaacttgg
      721 gattcattca tcaagttatt caaagcggta tgttcttcga tcgttccttt tgtacttgta
      781 aacaaaacac aaatcaatac aggaatcaca gaaagcaatc gtgtgactag tcttcttagc
      841 caaattggga tacgcatatg gatgaagcct tccatgatga cttgaccggt caacgtacca
      901 gtaatagtag agttctggcc agaagcaaga agtgctacag caaataaagt agacaagata
      961 ccagattttg ctacagcaat caagatacca ttgctcatag ctgatggatc agataaagct
     1021 tcatacagac caaaaaagga aggatcttgg acagaaccgg ttttgaatac agccacaccc
     1081 atgatcaata gcagtgcatt gactagaaaa gccattgaca actggatatt agaatcccaa
     1141 gtagaaaagc gtaccgcttg tgctacatct tcttcgtctt ggtgatcgat cttccgagtt
     1201 tgagaaactg ctgaatgaag atataagtta tgcggcatga ctgttgcacc aataatccct
     1261 aaagctccag tcagcggagt ttcgccgccg atgaccggtg agtctgagaa tgtgtgactg
     1321 ttagggataa atccggcaaa caaactgtgc caatcagggt tagacaagcc aacttgatag
     1381 acgaaaacga ataaaatgac gaaaatcaag cagacaacca aagcttcgat tttacgaaac
     1441 ccgacttttg tcagcaataa aaggacgaaa acatctaaca cagtgataaa tacggcaatt
     1501 accaatggaa tatcaaaaag caggtagagg gcgattgctg caccaatgac ttctgcgatg
     1561 tctgttgcca taatggctaa ttcagtcaag atccataaaa tgacacccaa tgtcttactt
     1621 gtccgtgctc ggattgcttg ggccaaatcc atttgggtca cgattcctaa ttttgctgcc
     1681 atatattgta aaagcatagc aatcagacta gacatcaaaa taactgacat caataaatat
     1741 tggaaatttt gtccaccagt gatcgaagtg gaccagttac ctggatccat ataaccaact
     1801 gctactaatg cacctggtcc agagtaagca agaagtgttt taaaaaaact ttttcccttg
     1861 ggtacatcca ctgttccatt tacttcagct aaagataaac catttgtatg ttcaataagt
     1921 cgatgcttat gaaattgttt gttggattga ttattcaaga aaaaaccacc tttcctattt
     1981 atctacgatt acattctaca cctcagaaag aaaaaattaa agaaaaagat ttggtatgcc
     2041 taattttttt tcgtggcgtg tcttgctggt taatgaaaga aattttgttc ttctctaaaa
     2101 aaaccactat tttttgcatg aaactaaaga atacgccttt cttttttcaa taaactttag
     2161 taaaataaat cattgagagc aattcggaaa tagaaaggag agatttatat ggtatcaaca
     2221 ggtattgtcg tacttattgc tattattgct ttgctaatcg gcgcagtagg tggctttttc
     2281 ctagcgcgta aatatatgaa agactattta gaaaaaaatc ctcccgttaa tgaggacatg
     2341 ttgcgatcaa tgatgatgtc aatggggcaa aaaccatctg agaagaaaat tcgccagatg
     2401 atgcagcaaa tgaagaacca agggaaaaaa taggttctga cccaaagact ttttgcgggc
     2461 tgattttttc agtccgtttt ttatttcgta atttgttaga ctattcagac tattcagcaa
     2521 aaaagacaag cacagacaat cctatgctct tgataaagac aaaggaggag taaaatgtcg
     2581 atttttagaa aattaggttg gttttttcga caggaaaaga aacattattt aattgggatt
     2641 ttctccctat tcgcagtggc cttggtacag ttagctcctc caaaagtgat tgggatcatc
     2701 attgatgaga tcgccgataa aaatttatca ataaagatca tttcctggtg gatcttatta
     2761 ttgatccttg cagctctctt acaatatctt tttcgttata tctggcggac gaatatttgg
     2821 ggaagtgcag ctcggttgga gaaagaactt cggcagcagc tattcaaaca ttttacagaa
     2881 atggacaatc tcttttacca aaaatatcgg actggagatt tgatggctca tgcaacgaat
     2941 gatttgaatg caatccaaaa tgtagcagga gctggtatct tgacctttgc agattcagtg
     3001 atcacgggcg gaacaacgat cattgcgatg attttgttta ttgattggcg cttaacactg
     3061 attgcgctga ttcctctgcc tttactagcc gttacttcac gagtgctggg gtcaaaactc
     3121 cacgatgctt ttcgtgattc gcaagaagct ttttctagta tcaatgataa gacacaagaa
     3181 agtattactg ggatcaaagt cattaaaaca tttggacaag aaaaagaaga tctcgcggat
     3241 tttgaagaaa agattgaaga tgcaattatt aaaaacaaac gagtcaattt tttagactcc
     3301 ttatttgatc cattcattac cttgattatc ggtctgtctt atgttgcaac aattatttta
     3361 ggtggaaagt atgtcttgga aggcacgatc tcaatcgggc agatggtctc atttattagc
     3421 tatatcggta tgcttgtttg gccgatgttt gcgattggtc gattgttcaa cgtattggaa
     3481 agaggaaatg cttcttatga tcgcgtcaat gaacttttgc atgaaaagac gcatatcgtg
     3541 gaacaactgg gtgcagtgca gactccggca aaaggcgagc tgaatgtagc tatttcctct
     3601 tttaactatc cgaaagaaga atcaccagca ttagaacaaa tcaaatttgc gatccaagaa
     3661 ggagaaacat taggtattgt tgggaaaact ggtgcgggaa aaacgacaat catcaaatta
     3721 ctgatgcgtg aatacgatga ttatcaagga aaaattcaat ttggcggatg ttcgatcaaa
     3781 gaatattcat tgaatgcttt attaggatca atcggctatg tgcctcaaga tcacttcttg
     3841 ttttcgatga ccattcgaga taatatccgt ttttctaatc caaaactttc agaagaagaa
     3901 gtagagcatg cggcttcttt agcatttatc aacaatgaaa tcaaggaatt accaaaaggt
     3961 tatgatacct tagttggaga acgtggagtc tctttatctg gcggacagaa acaaaggatt
     4021 tcgattgctc gagcattgat caaaaatcct gaattgctga tcttagatga tgcactttct
     4081 gcagtagatg ccaaaacaga agaagctatc ttgcataatt taaaagaaat aagaaaagag
     4141 aaaacaacca tcattgcagc tcaccgttta agcagcgtga tgcatgcaaa agaaatcatt
     4201 gttgtcgatc aagggaaaat cattgaacga ggaacccatc atgagctgct tgcattaggc
     4261 ggctggtatg aacgaatgtg ggagaaacag aaattagaag cgaagataga aggaggcgag
     4321 gcctgatgga tgaaaattat caatcggaat ggacaaaatc gattccactt aaagaacaga
     4381 agaagatcat caaacgactg atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg
     4441 ctatcttatt tgcgtttgcc ctgtctgtga tcaatgtcct attgccaagg atcatccaaa
     4501 cttttatgga tgatcatctg gctaaacagt cggctacgac acaagttatt ttgttttttg
     4561 ctggtatgta cttgtttgga gtcatagtca aaagtatcat ttggtttttc caatggtatt
     4621 tgtattccat ggcttcattg aagacctatc agtatgttcg ggtaagatta tttgaaaagc
     4681 tgcatacact aggtatgcgt tattttgatc agacaccggc aggatcgact gtttctcgag
     4741 tgacaaatga cacagaaacg ctatttgaat tttggtatgt ctttttgatg gtattgactg
     4801 ggatctttgc agtcgtttct tccttcattg ccatgtttca aatcaatgga gaaattgcct
     4861 tgtggacttt ggtgttcttg ccgattttag gaatcgtcat ctggtattac cagaaattca
     4921 gttctcgtat ttaccggaat atgcgggaaa aattaagtca gctgaacacg aaattgaatg
     4981 aatcgatttc cggcatgcgt attatccagc agtttcgcca agaagctcgt ttagctaaag
     5041 aatttgaaca agtgaacaac gagtatttag acacgcgcta tgcgatgatc aaaacaaatt
     5101 cgattctgtt aagtccgatc atcaattttc tatatgcatt ggcgattgcg ttggcgttga
     5161 ctttgtttgg gatcgatgcg ctgcattctc ccgtagaagt tggactgatc tatgcattta
     5221 cgacctatgt ccagggattt tttaatccaa tgtctcaaat gatggacttt ttgagcacct
     5281 ttacagatgg aatggttgcc ggtagtcgta ttttgaaaat catggataca gaagaatatg
     5341 caccgcaaca aaatcctgga gcagatcaag cgattacttc aggtaagatc gagtttcgca
     5401 atgtaagctt ttcttatgat ggagagcaca atgtattgaa caatatctct tttgtcgcta
     5461 atccgggaga gaccgttgca ttagtggggc acaccggaag cggaaaaagc tcgatcatca
     5521 acgtattgat gcggttttac gaattttatg aaggccagat cttgattgat gatcatgaca
     5581 ttcgagaata tccaatggaa gagttacgta agaaaatggg attggtcttg caagatgcct
     5641 tcttgtttta tggagatatc gcaggaaata tccggatgat gaatccaact atcacagacg
     5701 aacagattcg ccatgcagct gagtttgttc aagcaaacaa gttcatcgat gaattaccaa
     5761 aaggatatca ctcaaaagtg atcgaacacg gtgccagcta ttctagcgga caacgacagc
     5821 tgatcacttt tgcgcgaaca atcgtgacag atcctaaaat actagtattg gatgaagcga
     5881 cagcaaatat cgatactgaa acagaaggat tgatccaaga aggattgaca aaaatgcgac
     5941 aaggacggac aacgattgcg attgcccatc ggctgtccac gattcgggat gcagaattga
     6001 ttttagtttt agacaaaggt catatagtag aacgtgggaa tcatgaaaca cttctagcgc
     6061 aaaatggttt gtatgcagat atgtacaaac tgcaaagcag tggtgtgtaa aaagtggttg
     6121 tgacagggct ccgttcagct ttaggtgata agaaaaaaat tgtaaaaaag gctttccaga
     6181 tttttgcata aatttggctt atcaccgata agctggtact ggaacaccgt atattagaag
     6241 ttgggacagt agcatagtag cattttgacc taagcgctag agtgaaaata aaacagagct
     6301 tcccagattc ttcatctgtg agcgttagtg tcgtaattac cagatttaaa aaaaatcacg
     6361 acagctagtc agaagtgccc aatagccgct aagaaagcgt tgacaaaagt gcttgctttc
     6421 gataatataa agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga
     6481 tgagtctccc atggttccat ggacggcttt ttgggttttt ctttttgttt tggatacagc
     6541 cctatatatc atggaaagga gtaaaacatg ctgattgaga aagtactgaa taacaacgtc
     6601 gtaataacca gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc
     6661 aaaaagaaag tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca
     6721 aatgacggac tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta
     6781 gaaatttctt gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat
     6841 tcggtgtata ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga
     6901 atatttgtac caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc
     6961 atcgggaaaa aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat
     7021 gaagccggat ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccaacaagac
     7081 aatacctacc tgtttactac aacaatacag gatattctaa atatcgtccg ctattactat
     7141 aagattacat ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cctacgtttt
     7201 tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg
     7261 taccaggtta ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac
     7321 tttttagccg agatattcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc
     7381 catattgcgc gtttagtgca tgagaatcgt tgtaaataaa taaaaaaagg aggataaatt
     7441 aacaagaacg catgaaataa gggtagtgac attcagttgg ctaaaccttc cactaataaa
     7501 ttggagggaa tagaaatggg aaaatatcaa gaattagcga gcaaaattgt agaaaatgtc
     7561 ggcgggaagg aaaatatcaa tggtttgaca cattgtatca caaggcttcg tttcaaatta
     7621 aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata tggacggtgt tgtgacggta
     7681 atgcgagcgg gaggacagta ccaagttgtc atcgggaatc atgtgccagt cgtgtttgaa
     7741 gaggttataa aagcaggaaa ccttactttt gatgaggctg tttcaacaga aaaaatgaga
     7801 ccgtttgata tgcttattga tggaatatcg ggctgtttcc agccattcct tgctattcta
     7861 gctgctggtg ggatgattcg tggactgaca gcattacttg tcttcttagg cgcatttgac
     7921 cgcggttctg gcacatttgt tatgtttgat aatatcggtg attctgtatt ccaatttatg
     7981 cctgttatta tcggtttaac agctgcccgt aaatttaaag taaatgaatt cgtagggatg
     8041 ttgcttgggg cagcattaat gaatcctagc ttgtcgctgg aagctttgag tggtgcagca
     8101 gaagcaccac tgactaccat attttctggc actatttttg aagcacctat ttatcaaaca
     8161 gtattcggca ttccctggat tgcgcgtaac tacgcttctt ctgtcattcc cattatcttt
     8221 attgtgttgt tagctagcca aattcagaaa ccaattaaaa agttagtacc tgagatgatc
     8281 gcgaatttct tcgttccatt cttcaccgtt cttattacaa tgcccttagg ttttttactc
     8341 gttggaccag cattcacatt cgcaactgat attttaatgg ctggatttga aacactttta
     8401 gcactttcgc cagttattta tggagcaatc gtaggcttct tctggcaaat tctagtgatg
     8461 tttggtctcc attgggcaat cgtaccaatg ggattgatgc aattttctgt gaatggttgg
     8521 caaaatatca tgacacctgt tgcagtagtg tcttttggac aaactgcagc acttactgca
     8581 ctctacttta agcttagaaa cccaaaagac aaagcaatcg caataccagc cattgtttct
     8641 ggtattgtgg ggattacaga gccagctatt tatgggttta cattgccacg taaaaaaata
     8701 tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt atagtggatt aatgaattta
     8761 acttcttgga atccaggtgg attaggcatc tttacaattc ccaactatat tagaccagat
     8821 ggtgatttaa cggatgtgat taatgtattg attggaatcg ccattgcgat ggtggtatcc
     8881 tttgcgctga cttttttctt ttggaaggag gaagcaggtg aaacagatga tagacagaaa
     8941 agatcaggta aagaaattgt aaaaacaccg atacaaggac aaatcgctcc actgaatgca
     9001 gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag gaatcttgat ctaccctgaa
     9061 aaaggagaag tacgtgcccc ttttgacgga acagtcatga ccctgttccc gacgaaacat
     9121 gcaatcggaa tggtttctga agcaggtctg gaattgctga ttcatgtcgg attggatact
     9181 gttcagttgg aagggaaata ttttgaaagc ttggtgcagc aaggagaaag agtgaaacaa
     9241 ggcgatctgt tgctccgctt cgacaaaaag aaaatcgaag aggaaggata cagtttagaa
     9301 acacctgtga tcgtcaccaa ttacacggat tatttggata ttttggaaaa tcaaggggag
     9361 acggtcgggc cagacgatgc acttatcacc gcactgacct gaacggttag gtgaaagaat
     9421 ggactaggct taattattct ttttagtcca cttctttatt ttcaaaattg aaaggctctt
     9481 tcatgatggc tgagctgata gggcgtttat caaatttttg tcctacagaa attagatgga
     9541 ctaaattctg ttgataattt tctgcaaaaa cacaactata taacacgtct agaagataac
     9601 aaatcgatgt attgatcgta aaattaccaa ttttcgaata cagtcgttcg cgtgtcgtaa
     9661 gtcgcaaagc ggctgtactc attgaagata gcgttgaatc accgatactg gtcaaagcta
     9721 aaattggaac atgattttgt ttcaatattt ctgcggtacg aaggatcaca ttgttttcac
     9781 ccgtataaga aatcagaatc ccgcacatat cactcgtgca attgtacgct tcatagaacg
     9841 attcaccaaa agtggtgaca atcgtgacat ccattttgat gcgcttcatt ttcagcatga
     9901 aatcttgaga aatcaaagta ttcgagttac tggcgaaaat cacgattttt tttgcttgaa
     9961 gcattagttg tttggctttt tgcagttggt catgatgaat gagagaaagt gtatcttgaa
    10021 tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt cataataccg tctgtggtgg
    10081 aaaaaggaag attcgcatct acgtcttgaa aattcccttc aagataggct gtttcagcta
    10141 aaaaagcatt acgaaaactg acccagccat caaaacccat tttttttgct acacggatga
    10201 gtgtggacgg gtggacataa catcgctcag caatctcttg gatggtcatt tcgccaagtg
    10261 ctggagaatc gcttaagaga aactgaatca cttcattttc tgaaggagaa aaggagattt
    10321 ctttgatttt ttcacgtaat aacatgtaga taccttcttc ttgttttttt acgtaaatta
    10381 taactttttt g
//
LOCUS       DAYYAR010000095        10171 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000095 DAYYAR010000000
VERSION     DAYYAR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.095"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            353..622
                     /gene="rpsN"
                     /locus_tag="ACHAT0_002071"
     CDS             353..622
                     /gene="rpsN"
                     /locus_tag="ACHAT0_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8451999.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            637..816
                     /gene="rpmF"
                     /locus_tag="ACHAT0_002072"
     CDS             637..816
                     /gene="rpmF"
                     /locus_tag="ACHAT0_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8452000.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            848..1225
                     /locus_tag="ACHAT0_002073"
     CDS             848..1225
                     /locus_tag="ACHAT0_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452001.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1246..1395
                     /gene="rpmG"
                     /locus_tag="ACHAT0_002074"
     CDS             1246..1395
                     /gene="rpmG"
                     /locus_tag="ACHAT0_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8452002.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1419..2402
                     /locus_tag="ACHAT0_002075"
     CDS             1419..2402
                     /locus_tag="ACHAT0_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8452003.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2432..2554
                     /locus_tag="ACHAT0_002076"
     CDS             2432..2554
                     /locus_tag="ACHAT0_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8452004.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2572..4095
                     /locus_tag="ACHAT0_002077"
     CDS             2572..4095
                     /locus_tag="ACHAT0_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8452005.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4119..4358
                     /locus_tag="ACHAT0_002078"
     CDS             4119..4358
                     /locus_tag="ACHAT0_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452006.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4551..5114)
                     /locus_tag="ACHAT0_002079"
     CDS             complement(4551..5114)
                     /locus_tag="ACHAT0_002079"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8452007.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5207..5425)
                     /locus_tag="ACHAT0_002080"
     CDS             complement(5207..5425)
                     /locus_tag="ACHAT0_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452008.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5614..6561
                     /locus_tag="ACHAT0_002081"
     CDS             5614..6561
                     /locus_tag="ACHAT0_002081"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8452009.1"
                     /translation="MYGDHGKEIAVIVNDIGEVNVDGVLVDKKGFKRTGEKLVSMPNG
                     CICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPIIQAIILAKNPLDIDLTQRL
                     EIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDIRGLLIDQFECCNILLLNN
                     SDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILNTNLFNIDETSYSMGWFKE
                     LELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHS
                     DFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6528..6617
                     /locus_tag="ACHAT0_002082"
                     /pseudo
     CDS             6528..6617
                     /locus_tag="ACHAT0_002082"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6750..7157
                     /locus_tag="ACHAT0_002083"
     CDS             6750..7157
                     /locus_tag="ACHAT0_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8452010.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(7398..8738)
                     /locus_tag="ACHAT0_002084"
     CDS             complement(7398..8738)
                     /locus_tag="ACHAT0_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8452011.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(9014..9169)
                     /locus_tag="ACHAT0_002085"
     CDS             complement(9014..9169)
                     /locus_tag="ACHAT0_002085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM8452012.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9176..9322)
                     /locus_tag="ACHAT0_002086"
     CDS             complement(9176..9322)
                     /locus_tag="ACHAT0_002086"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452013.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9353..9556)
                     /locus_tag="ACHAT0_002087"
     CDS             complement(9353..9556)
                     /locus_tag="ACHAT0_002087"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8452014.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            complement(9466..9798)
                     /locus_tag="ACHAT0_002088"
     CDS             complement(9466..9798)
                     /locus_tag="ACHAT0_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8452015.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3388 a         1617 c         2006 g         3160 t
ORIGIN      
        1 aaaaaattgg cgataaacca gttaaacata aaagattgaa taaaaagtta tagtttcttt
       61 catattgccg ttttaagtag aggaaaagct acttaaaacg gtatttgtgt ttgattggac
      121 taataaaagt gaattcttat ttttcagagc ttaaactgca tattctttta aatgatttga
      181 ttatctctat gtaccgataa attcgttttc aaatagttat ctattcatat gagttttatt
      241 tcatgttatt tattgttgac aagagcaact cttgttggta aagtatcagt gtcaaaacgt
      301 aacgattacg atttgagatg tctaaatcaa aataaaagga gtataaaggt atatggcaaa
      361 aaagtcgaaa attgctaaag caaagaaaca aatggcgatg attgaaaagt acgcagataa
      421 acgtcaggaa ttaaaagccg caggagatcg caccgctctc gctaaattgc caagagattc
      481 aaatccgaat cgtctgaggc ttcgtgatca gacggatggt cgtccaagag ggtatatgcg
      541 aaagttcggt atgtcccgta ttaagtttag agaacttgcc catcaagggt tgattcctgg
      601 agtaaaaaaa gcaagttggt aaaaggagga aatatcatgg cagtaccagc aagaaaaaca
      661 tctaaggcga agaaacggtt aagaagaact catcaaactg tagtaaaacc agaaatttca
      721 tttgacgaag cgaatggtga ttatagacgt agccatcatg tatctttaaa agggtactat
      781 aatggaaaga aagtaattaa aggagcttca aaatagttta atatataact tttaggaggg
      841 aatcaaaatg gaaattattt acccaccttt agttgaagaa ggattaaagt attatcttga
      901 aacgacccag caatcgctag ataaatgcac tttttatcgt tcgatggtag agagaggaat
      961 tattactgaa acgggtttac cgacccaaca ggcaattgaa aatggattag tcaaagatta
     1021 ctatgaggac caaggcttat catttgatga ttttttaaga atctatccaa tttttgaaga
     1081 gtatgacgag gagttatttc aatgtattga tggttattgg gagatacctg ttgatatgaa
     1141 agaaaattta gtttcacaat tggaatctgg cgagttaact tttgaagatg cacaacaaat
     1201 tcaagcatat cttgaagatc gataagtatt gaaggaggaa gatttatgcg tcaaaatatt
     1261 attttggaat gtgtcgaaac tggagaacgt ctgtatctca ccagcaaaaa taaacggaat
     1321 aaccctgaac ggttagaatt aaaaaagtat tcgccaaaat tgcgcagacg ggcaattttc
     1381 aaagaagtga aatagctaag tggtaaaaag gtgacaaaat gggcatacca gttacaatcg
     1441 tttctgggtt tttaggggct ggaaaaacaa cgctaatcaa tcaagccttg caagtctcac
     1501 cctaccccag ggaagaaatt attataattg aaaatgagtt tggtgaagtt gggattgatc
     1561 acaaattatt agttcatagc gaggaacggg tgttacaact aaataatgga tgtatgtgtt
     1621 gcagtcttcg cagtgattta ttagcaatat tgatttcttt actggaaaca atagaagaac
     1681 atcaacaacc aatttcacaa atcatcattg aaactacagg tatagcagat ccccaaccaa
     1741 tcattcaaac acttttaaca gcgcctcaac taagaggaag agtatatatc gatagtttat
     1801 tgactgttat tgatagtgac agttttgaac ttattgaaag agaaccacaa gcccttaagc
     1861 aacttgtcat agcagatcgc atttttgtgt ctagaaaaca aaacttgaat ttatctgcac
     1921 taaaaaaatt acagagagaa atcaaaagta ttaacccttt atccgatatt cgaatgttca
     1981 cacatagtga aacgattcaa aaaaaggatt tttataattt ggaaaaattc aatcatccct
     2041 tagtttttga aagtgattca tcagaaaatc actataatgg caaacactat catcaacatc
     2101 atcaagaaca tgagtttaaa tcaattctct taacttcaga tcaagacata aaaaaagatt
     2161 ttttattcca attaattgac cggctgattt taaccaacca agggagcctg tatcgtttta
     2221 aaggattgat tgctttgcaa gatcaagatt ttatagttgc catacaaggg gtaaacgaac
     2281 aggttaattt tcaatacacc actcgacaaa aagaccaaac cactattatt ctaattggaa
     2341 aaatgttgga tgaagaaagg atcaaacaga gttttgataa attactggcg gaaagcaaat
     2401 aatgataagt attgattagg ggtaatttct aatggagaaa acggatatat ctagtgcata
     2461 tcgacgactt aaaagcccga atatcaaaac tagaaaaagg gcattaaaaa ttatcaaaga
     2521 acataaacaa aataaaatga agaaattagc ataattaagg aaggaaaaag aatgaagaaa
     2581 atcagtgtag gacttattgg gatagcagct ctaggattat tgggagcttg ctccagcact
     2641 aatgatgcaa aagtatcgaa tgataaagat ggaaaattag aaattgtaac aacattctat
     2701 ccaatgtatg atttcaccaa aaacatcgtc ggcgatgaag cgaacgtgga tttaatggtt
     2761 ccagcgggat cagaacctca tgactatgag ccctcagcta aggatatggc aaaagctcat
     2821 gatgctgatg tttttgtata tcataacgaa aacatggaat catgggtacc aaaagctaaa
     2881 gaaagttgga agaaagctgg gcctaatgta gtagaaggaa caaaagacat gattttattg
     2941 ccaggtagtg aagaagaaga tcatgatcac ggagaagaag atcaccatca tgaactagat
     3001 cctcatacgt gggtttcacc caaaatggca attaaggaag tttcaaatat caaggatcag
     3061 ttagttaaat tgtatccgaa aaaagcaaaa gtatttgaga caaatgctga aaagtatttg
     3121 actaaactga aacggttgga tgctgactat accactagtc ttaaagaagc gaaacaaaaa
     3181 agttttgtga cacaacatgc agcctttgga tatttagctt tggattatgg attgatacaa
     3241 gttcctattg ctggactaag tcctgaagaa gagccatctt ctggtcgatt agctgaatta
     3301 aaagagtatg ttaaaaaaaa taagatcaat tatatttact ttgaaaagaa tgctaatgat
     3361 aaaatcgcaa aaactttggc caatgaagct gggataaaat tggaagtatt gaatcctctt
     3421 gaaagtctta caaaagaaca aatggataat ggtgaagatt atgtttcagt aatggaagac
     3481 aatctaaaag cgttagagaa aacaactatg gttgctggtg aagaggtagt tccagaaaaa
     3541 gaagcgaagg atgaaaagac agttgctaat gggtacttta aagatgccga tgtgaaggat
     3601 cgcgaattga gtgattatac aggcgaatgg caatctgttt atccgttgtt aaaagatgga
     3661 attttggatg aagttttcga ttacaaagca aaactcaata aagacatgac agctgccgag
     3721 tacaaagatt attatactac tggctataag acagatattg atactatcaa cattaaagac
     3781 aacaccattg attttgtagt caacggtgaa catcatcaat acacatacaa atatgttgga
     3841 tacaaaattt tgaattatga aaaaggaaac cgtggtgttc gttttaattt tgaaactgat
     3901 gatgcaggtg ctggtcgttt taaatatatt caatttagcg atcatggaat tgcaccaagt
     3961 aaggcagagc atttccatat tttctttggt ggagaaagtc aagaaaaact ttataatgaa
     4021 atgcataatt ggcctacttt ctatccagct tcattatctg agcatgagat tgctcaagaa
     4081 atgatggccc attaaaatcc aatctttgga ggaaatacat ggtaagaaaa gtattattgt
     4141 ctatggattc caagaatatt gatgcttatc ggcgttatca atttgcaaag caaatgagat
     4201 taaatgggag tacattagct gatattgctg caactttaca tgttagtatt gacacagttc
     4261 atagagtatt aaaacgggat cctgattgtt gttttatcaa ggtcaatact ggaagaagta
     4321 gacgtttagc aaaacggcat aatagcttgg ccaattaatt gataatacca gagtagatca
     4381 tttttccctt atttggattt gatctgcttt ttattatttc ttgaattgtc aaatcaagcg
     4441 caacacttta tcaaagaaga cttcataagg tgttctccaa tctaaacact ttctaggtct
     4501 gttattcagt ttttcaatca tctgatttat cgttgagtta tctaccgtag ctatctcttt
     4561 cgattttggc aagtattctc gaatgagtct attcgtattt taatttgttc attgttgcca
     4621 agggcatgag gatctggaaa ataaaagggc atattattca atacttctgt aactgcgaaa
     4681 tgattggaga attcttttcc tctatctgga gtgatcgttt tgaccgcctc atttggaata
     4741 ctgcttaaaa gttcaatcat tttctcgctc acataagcgg ctttctttct ggggaccttc
     4801 tttcctgcta aaaggaagca tgattttctg tctgtcaaag tcactaaaca agtaccacta
     4861 gttttgccag ctacaatatc ggctttccaa tgaccaaaat cacttctatc atctgcggat
     4921 tttggatggc catggatcga atgagtaagg caaatcttcc cacgagactc gacatgattt
     4981 tttgtatgtc ttgtttttcc tcgatggtgc aatgaacgga tcaggccttt attgccgtgt
     5041 gagaggggtt ctgtatcgaa aagatcattg caaattgcac ggtagatcgt gataaaacta
     5101 attgaaaagt ccatatgttc atgcttcaaa cgattcgaga ttttctctgg cgaccactga
     5161 aaatcgacaa ataaatgacg aacaattgac caaactcacg gatcatttag aatgtgataa
     5221 cgtccgcatt tttttcttcg ataagcataa tttttttccg ctttggaagg tgattagttt
     5281 ttttcttgtt tcttctcagt tcatgtaaaa tagtaaacgg gctatgctga atgtcgttgc
     5341 caattatact aatagaaaat ccttcttcgt gaagcagaaa gattctttct cgtttgagta
     5401 tagtaagatg acaatactgg ctcataatta ttttccttct aggagtgttc tcgacaaact
     5461 tatactagtt ggatatcgat tatgactcat ttttatgtgt tgcacttaaa gtgtaaattc
     5521 aaggtataga aagaggttcc ctatgaaaaa atacctatta caatcttaac tggctatctt
     5581 ggttcaggaa aaacgatact tctaaatcat attctgtacg gtgatcacgg aaaggaaatt
     5641 gctgtcattg taaatgacat aggggaagta aatgttgatg gggtattagt cgacaagaaa
     5701 ggttttaaaa gaactggaga aaaacttgtt tcgatgccga atggatgcat ttgttgtaca
     5761 ttacgcgaag atttgattgt tgaactagat caattagtgc aattttctga cttgaatcaa
     5821 attgttattg aagcttcagg aatcagcaaa ccaattccaa ttattcaagc aattatatta
     5881 gctaaaaacc ctttagacat tgatttgacc caacgattag agattggtgc cattattata
     5941 gtagtggatg taaaacgttt ttgggataat ttcgaaacgg gtgaaaagat tcaggaaaga
     6001 aaagctgaag gtaatgtagt tgaagaagaa caggatattc gtggcttgtt aattgatcaa
     6061 ttcgagtgtt gtaacatatt acttttaaat aatagtgact tagtgtcaac caagacgatt
     6121 tacaaaatta acggtctaat tgctaagtta cagtccaatg ccaaaataat taggacaatt
     6181 aaaggtgaag tagacttatc gaaaattcta aatacaaatc tttttaatat agatgagact
     6241 tcctattcaa tgggctggtt caaagagtta gagcttggct atcaaaatca tattcctgaa
     6301 acagaagaat atggtatctc aagttttatc tatcgaaatc ggttactcct tgacgcacaa
     6361 aaatttgccc atcttacgga taaaaatttt cctggcaata ttacaagagc aaaagggttt
     6421 ctttggtttg cccaacatag tgatttttcg acgatctttg aaacatcggg tagccataaa
     6481 agtattgaat cattaaatta ttgggtagct agtttaccta aaattgagtg tcaaatgatt
     6541 atacaaaaaa acccgaattg aaggaagcct ggcatccgtt gtttggtgaa cgcattaata
     6601 aactagtttt tattggtatg aagatggatc aacaatgtat taaggaacaa cttgagggtt
     6661 gtttaactga ttctcttaat gcagataatt tccgaaaaat ttcaccattt ccatggaaaa
     6721 attctaatat gtttagccag atatcgttaa tgaaattttt aagaaaagct ggtgtcaaga
     6781 taggcgatgt cataattatt ggcattttga ttattccctc ttttattccc ttatttgtat
     6841 tctctataga aaaggagaat gaaacaactg aagatgctat caaatatgcc atcgttcaaa
     6901 ttgatggcaa ggaaatggat cgcttcgatt tggataagat caatcataaa ctcgtgaatt
     6961 atcatcctgc gaagggacaa tataatattg ttgagattaa agatggaaga attcgagtta
     7021 aagaagataa tagtcctgat caaatcgcgg ttagaactgg atggatctca aaacccggtc
     7081 aaacgagtat ttgtttaccc cataagttag ttattagtat tgagaaaaaa gaaagcaaag
     7141 attactacat ttactaatct ggaaatagat gaggaggttt ttaataagaa aaagatatta
     7201 ttattaataa tagcaggggc tttattaagt gtgttaagcg cttgtggaca agaaaaagaa
     7261 gcggtgacaa caggcacaag tatctttaac gggacctgca atggtttagt taacagtcgg
     7321 ttagattact tattttggct cttcgtcaat gagtgttggt gaagaaattc aatagaataa
     7381 tcgagatact cacacggcta gacagctatc ttgtctagcc gtgttgttgt tctggcttta
     7441 gtcagttggg tagtgttctt agttttcttg aaatattttc ctcgatataa aaagattcgt
     7501 tctcggtagt tttgaaagtt tctaaagcca taggcaattc gttttagtac tttgatatgg
     7561 ttgttcaaac attctatagg gccgttggaa tagggaactc gcaacgctcg tttgatttct
     7621 gttcgatatt tcttgaaggt cgtgaacact ggttgatagt gctctggcaa ctccttgtag
     7681 tcttctttta ggagcgcgac gaattcagga acatcttgtc ttctgatagc ggaaagaaaa
     7741 acctggtaga cttcgtagcc tcgctttaag gagtcatcat aatataatag ccgatcgact
     7801 atctccgctt ctgtcagatg atctcgaaat gacggacgcc agtagcgatg ctgaccattc
     7861 aattcccacg cattcttttg aagtagtctc cagtatttct ttaagtgctt cgaccgacga
     7921 tccccttttt taaaggtctt cattacttga attcggtggt tggtaaaggc ccgaccgatg
     7981 tgttggctaa tatgaaagcg atcggcgacg attcgggcat tcggaaaaca ctctttcacc
     8041 aatgtgcgat agggtgtata gaaatctgaa accactagac gtacttgttc acggactttt
     8101 ctagggtagc gcaagaaata gtcgcgtaaa ggattgagtt gccgattttc cacaatatca
     8161 atcagttgat gggtcttgcc gtccatcatg acaaagctca tggctcccga gacgtttttc
     8221 accgatttga attcatcgaa acacaccact tctggtaacg gctggttcaa agaaacggtt
     8281 ttaggccgaa ggctgcgaag tattcgaatg acagtggtcg gagaaatgtg cttcatacgt
     8341 gcaatcaaag acatagaaat aggctcactt aatagctcaa gaatggcttg tttgactctg
     8401 cgggcaatgg agcagcgacg atcgctaact gaatcttctg ctaaaaaagt ctttccacag
     8461 tgtttacatt tgaagcgctg tttgttaatc cgcacaatgg tcttgtagtt actgacatca
     8521 ttcaataaga tggatacttt tttccagccc catttaatga tcgtttgctt attttgatag
     8581 tggcaatgga agcattcctc tggttgatag gtcaatgtgc catcaaaaac aaaacatgtt
     8641 tcgtcattaa tgcgctcttt gcgaaaggca tcttcatgaa aggtaagatt taggtctaga
     8701 atatctagta actctttggt aaactctgaa taggacatct tgaaacactc ctttgaatgg
     8761 ttttcgtcga ctttattcta aaggatttca aggtgtctgt ttgcgtttat tagaaaaaag
     8821 aaaaagtatt ggcaacagaa ggcgtcacca acactagata ttatagatcc cttattttct
     8881 tcgcttcaaa ttgtcaagga gtcatcataa attttgtatg gattcttttg tttgggtagt
     8941 agtcttctaa ataatcttgg attaacacct tcatctctat tttactttta tatttgttgg
     9001 agtcgattaa ttctcatttg attgatcgat gaaacgcttc aattactgca ttgtcaacag
     9061 gagtcccagt ttggctcatt gaatgtgtga tgtggtggtc ggtcaacagc tcttgataaa
     9121 taaaagaccg atgctggcta cctctatcgg aatgaattac cgtaggcaat tctccttaat
     9181 aaatttgtaa tggacggacg acaatctctt ttttcatgtg tgaatccacc atatagtaac
     9241 gtagaatacg agttgctaag tcaatatagg tggacaaata gaggcgaccg tcattacatg
     9301 gaatgtaagt aatatccgtc acccaaacag catcaatttg gttctgttca aattatcgat
     9361 tcaacatttt ttttaccatt tctttgattg ttttttctct accatgagaa gatttgcgtc
     9421 gatgaaatcc tcttgtgcat aaattcattt cacgcataag aacagctaca acacgtttat
     9481 tgatttcgat tccttcatcg taaagttttt gagtgatccc tggagatccg tatcgatctt
     9541 tgtgttcgta gaacactcgt cttcctcttt tagtcgttta ttttcctgtt caagaatgta
     9601 gaaatctgct ttatcaggta atctttttcc ttgtccagca aaagcatttt tcccgtatcg
     9661 acgatattct gaaacccatc gggaaattgt tggacgagag atattgtatt cttcagcaat
     9721 tttcttggta gttacttttt tctcaaggat atcctgtgaa atctttagtt taaatttacg
     9781 atcatatgtt tttttcaata gagttcattc cttccggtaa cgtaactgat agtcaagaaa
     9841 agtgtatcat agcccaactt agtatcaggg tttgtcaact aaactgtgga agttgataga
     9901 taagagtttt taacaaccaa aattctcttg actatttttg aaatcagaca aattttgatt
     9961 ggacacatag ttgaataaat ctaccgtcgt tacggaaggt aaatcgcatg cttttcaatt
    10021 ctagaggaga agtcctcgtt gaagtaccat ggcccagttc tggattcgac ctcctgccca
    10081 cttcgtacgt aaataaagta attttaggag tgagactgtg acaaaagtta tttttaagct
    10141 aaaaacgaat taaaaaacgt taataattaa a
//
LOCUS       DAYYAR010000096        10168 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000096 DAYYAR010000000
VERSION     DAYYAR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.096"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="ACHAT0_002089"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="ACHAT0_002089"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8452016.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="ACHAT0_002090"
     CDS             2370..3848
                     /locus_tag="ACHAT0_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8452017.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="ACHAT0_002091"
     CDS             3814..5145
                     /locus_tag="ACHAT0_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8452018.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="ACHAT0_002092"
     CDS             5259..7811
                     /locus_tag="ACHAT0_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8452019.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="ACHAT0_002093"
     CDS             complement(7818..9257)
                     /locus_tag="ACHAT0_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452020.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9232..9414
                     /locus_tag="ACHAT0_002094"
     CDS             9232..9414
                     /locus_tag="ACHAT0_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452021.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9603..9965
                     /locus_tag="ACHAT0_002095"
     CDS             9603..9965
                     /locus_tag="ACHAT0_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8452022.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3234 a         1671 c         2150 g         3113 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 ttcttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaa
//
LOCUS       DAYYAR010000097        10092 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000097 DAYYAR010000000
VERSION     DAYYAR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.097"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(558..782)
                     /locus_tag="ACHAT0_002096"
     CDS             complement(558..782)
                     /locus_tag="ACHAT0_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452023.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            973..3750
                     /locus_tag="ACHAT0_002097"
     CDS             973..3750
                     /locus_tag="ACHAT0_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8452024.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3831..4250
                     /locus_tag="ACHAT0_002098"
     CDS             3831..4250
                     /locus_tag="ACHAT0_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8452025.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4262..4732
                     /locus_tag="ACHAT0_002099"
     CDS             4262..4732
                     /locus_tag="ACHAT0_002099"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8452026.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4749..5528
                     /locus_tag="ACHAT0_002100"
     CDS             4749..5528
                     /locus_tag="ACHAT0_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8452027.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5515..6336
                     /locus_tag="ACHAT0_002101"
     CDS             5515..6336
                     /locus_tag="ACHAT0_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8452028.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6353..7402
                     /locus_tag="ACHAT0_002102"
     CDS             6353..7402
                     /locus_tag="ACHAT0_002102"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8452029.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7415..8419
                     /locus_tag="ACHAT0_002103"
     CDS             7415..8419
                     /locus_tag="ACHAT0_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8452030.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9052..10071
                     /locus_tag="ACHAT0_002104"
     CDS             9052..10071
                     /locus_tag="ACHAT0_002104"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8452031.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3390 a         1660 c         2099 g         2943 t
ORIGIN      
        1 tctataaatg aattctgcat aaaaaatata tcaagtaaat gtttatagat gaaactctat
       61 ttacttcttt tttaatttgt tcttgcaatc ttttttgcta cgtaattttt gtaaaaaagt
      121 gggttgagag aaatctaatt gattttcaaa gaaggagtat gaaaatgaga aaaatattta
      181 ttcgtttatt gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg
      241 gcgcagattc tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagcc
      301 acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta
      361 ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga
      421 tggtgacaac agtacctctt tactctctga aagcatcata actcgttttt cctcattatg
      481 ccaataaatt gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa
      541 gttgaaatct gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata
      601 aagatgaata aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt
      661 tgttctgtca tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg
      721 atgatttctc gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc
      781 attgaatagt catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga
      841 aggaactgta cgttttttag aataaaaatt ataagggata agtaggctgc tgtctataat
      901 aagatatatt tttcaattct ttattaaaga ttttattgac aaaatgaatc cgttttctat
      961 gatggaggag gggtgaataa gttgttgaag aaggaaattt tatgttactt agagaaccag
     1021 acagcctttt tcgatttaga acatatgaac gaggtcttta cagcacaaag gttagctgat
     1081 cattttgggg taaaaagaaa cacagtcagt cattatttga accagttaaa tgatgagcaa
     1141 ctactagtca aaatcaattc tcgaccagtg atttatttcc acaaagaagc ttttgaaaaa
     1201 caatttttta agttacgcac gaatttttat ttttctatcg atgatctcaa agaggaacag
     1261 ccttttttcg cgcaaccaaa agacttattc tcttttatga tcggatacga cgccagtttg
     1321 aaagaaagta tcgaacaaat caaaacagct ctttactatc cagatggcgg attaccactt
     1381 ttgattacag gcgaaagtgg cacaggtaaa agttatttag ttaatttggt ctatcagtat
     1441 tgtctgcttc atgatctgct agaagattct gcaccattca tcacttttaa ttgtgcacaa
     1501 tatgcagata atcctgaact tttgactagc aatttgtttg gacatgttaa gggagcgtac
     1561 actggtgcag aagaaaataa aaaaggggct tttgaagcag cagatggtgg gatcttgttt
     1621 ttagacgagg tacatcgctt aacaccagaa ggtcaggaaa aactgttcac ctatcttgat
     1681 caaggtgtga tttatcgtat gggagaacct aatttgtcac gccggatcaa aacacgtctc
     1741 ttttttgcta cgactgaaga gattactagt catttcttaa caactttcat tcgaagaata
     1801 cctatccgaa tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg gctagaactg
     1861 atttactctt tttttctcga agaacaaaga aaaatgggga gccctttgtc agtcagtgga
     1921 ccagctttgt cacttttaac aagtcagaaa ttatccggaa acataggaga gctgaaaaat
     1981 atcgtcaaag tttcagcagc aaaagcatat gctgaacagc gtgaacagcc tgaaatccat
     2041 gtcacgatcc atcatttgca aaaagaacta cttgcgcagc ctattactaa aaatacagca
     2101 cagcaaggaa tctatattac gcaagaccag accttagagc aattaatgga gaagcaacag
     2161 cccgaacagc agcgaatcgt taaaagtttt gagcaaatcc taattgattt caagaaaaat
     2221 aactgtctgc tatattcttg cgaaggaaaa ttaaagcaag aagtgattca attatttgat
     2281 tttttgctgt ttgagaccag tagacaagaa aaacacgagc tgctggttta ttggacacaa
     2341 tctatccgag agacctttcg acaaatggaa actgcctatc aaatcaaatt cgatggaaac
     2401 agtgtctatg ccttgagtta ctatctctat cagcgaagca ctatcaaatg gttaccggaa
     2461 gaacatgata ttatccaact aatcaaagaa ttagaacaac aagtttctca gtcttatcca
     2521 tctagttatc actatgtcca acgaattctg gaattaagta agatcaaatt agatattgaa
     2581 gacacaagta tggatcggat tttactgacg atctatctaa aaaaagcgaa atggtcaaaa
     2641 gaaaaacgac tgccaaaagc gatgattgct gctcacggtt atgcaactgc tagcagtatg
     2701 gcaaatgtag taaatcgttt attacaagag gatctatttg aatcatttga tatgccacta
     2761 gatgtcacac cacagcagat tgcttcagaa atccttgatt attgcgaaaa cagcgatgtt
     2821 tcaaatggat tgatcatatt agtagacatg ggatctctga aagaaatcca tcagtttttt
     2881 aaaaaacagt tatccgtacc gcttttgatt ttgaataatg tgacgacacc tttagcaatc
     2941 acagtagggg aatgcctgca aaaaaatgta tcattagaag agatagcaga agagactgtt
     3001 cgacagatcc agccagaatg gcgactgctc tatccagagg aaaacaaacc caaagcactc
     3061 attactactt gcttcactgg aatcggtacg gcggtccact taagtgaact gctagaaaaa
     3121 agcttaccta cggcttgtca gttgaaaatc atcccatatg aatatcagca attaaaagat
     3181 aagaaaaaca gtgacccatt attttccata tacgaagtcg taggaatgat tggcaccaca
     3241 gatcctgata tcaccaatat cgactatctt tcattagaag acctcatctc gggggaaaag
     3301 atgtctgtac tagctgaatg gttggacact actatgaatg cttcagagaa ggaaatattc
     3361 aatcagcgca tcattcgtaa tttttcattg gaaaaagtac tggattccgt gactattcta
     3421 gatacagaga aggtgatggg agaaatcgat cattttatga gagaattaga ggtgcagttg
     3481 aatcgaagca tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag ctgtctcatt
     3541 gaacgattga tccgacaaat gccaatcgaa acctatcaag gattagagaa atggaagcag
     3601 tgtcaaaaag aagggttatc tgctattaaa tctgcgttta gtgtcattga agagcattat
     3661 agtgtcatga tccccaattc tgagattgcc tatatctatg atattttgag tgaagataca
     3721 gagtttctct cgatagaaga agaattttga acataagcaa gttgttgcca ctaattttgg
     3781 catgcaactt gcttttttag tatgtgtaag tgaaaagagg agatgaaaaa gtggatagag
     3841 aaattatctt agcttcccat ggaaaatttg cttcagggat attagattca ttagagctga
     3901 tctatgggaa aaatcattct attactgtat tcgattgcta tactgatgag aattttgatt
     3961 tagcggaaac agtaaggcag ctttttatga ggtacaaaga taaagaaatc attgttctga
     4021 cggatctttt tggcggaagt gtcaacaacg aattcttaca atatatcaat cgggatcatg
     4081 tctatctcgt tgccggattg aatttgccac tattgattga gctagtatct cggttggacg
     4141 ctgaagtaga gacaactgtt ttgatccagc aggtgctaac tgattcaaaa aaaatgatcc
     4201 agttttgtaa cgagcggctc aatcagaaaa tagaagaaga cgaattttag gaggaagaaa
     4261 aatgattgta ttgacaagag tcgaccatcg gttattacac ggacaagtag ctttttcatg
     4321 gacacaaaca ataggcgcag actgtatttt gattgctaat gatgatgtac ctacgaatga
     4381 gatacgaaag acaacgatca aattagccaa gccacaagga gtcaaactag tgatcaaatc
     4441 aattgatgac tcgattgccg cgttgaaaag cggggtgaca gataaatata agttattcat
     4501 cgtagtggaa tcgatcgagg atgcatataa gctggcagaa gcgtatccag aaatcaaaac
     4561 catcaactta ggagggatca aggcaaaacc tggtactcgc agcatcggaa aagcggtaaa
     4621 tattttacca gaagaggagg aacgcttgaa acgattaatt gacaaacaaa tagaagtaga
     4681 aatccgtcaa gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt aagaaaggaa
     4741 gaacctcaat gattgtacaa gcagtcttat taggaattgt tgcatttgtc gcacaatcag
     4801 aatatgcact agggacatcg ttgctttctc gtccgattgt gacggggctg tttacaggaa
     4861 tcgtgttagg tgatgtaaaa actgggatca ttatgggcgc aactttagag ttagcattta
     4921 tcggatcgtt ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt gggattttag
     4981 ggacggcttt tgctattaca gctggagccg gtacagaaac cgcgctttta ctaggtttgc
     5041 cgattgctac gttgacattg atattgaaaa atatttactt agggcttttg attccaatta
     5101 tgaaccataa agcagacaat tatgcagaag aaggaaatta taaaggagtc gagcatatgc
     5161 acttgcttgc cggtatcggt ctttccttga tgcttggtgt tgtggtaatg gtttcttata
     5221 tggtgggaag caatacgatc ggaaatctat taaatatgat tcctgacttc gttcaaaagg
     5281 ggttgtcagt agctacaggg ttgattccag ctcttggttt tgcgatgctt gctcgtttgc
     5341 tgatcaacaa acaagttgca ccatattttt tcttaggttt tgctatagct tcttacttag
     5401 agattcctgt gaccggagta gctatttttg gtgcgatttt agcagttgtg gtagtcaata
     5461 ttatgaatgt ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa gaagatgaag
     5521 aattctgaga tcaaaaacag caaaataacg aaaaaagagc taaatcacgt attttggcgt
     5581 tcttttcaaa tggagttttc ttggaattat gaacgtcaga tgaatatggc ttatgtttat
     5641 gcaatgattc cgattttgaa aaagttatat cagtcgaaag aagaaatggc agcagctttg
     5701 aaacggcatc tagagttttt taacacgacc ccacatatcg ttacactgat tttaggaatc
     5761 acggctgcaa tggaggaaga aaataaggat gatcctgaat ttgatgtgac tgcgatcgat
     5821 agtatcaaaa catctttgat gggacctctt gcagggttag gagattcttt cttttgggga
     5881 actttgcgat tgattgccac aggtgtgggt acctctttag ccatgcaagg aaatatttta
     5941 ggccctatct tgttcatttt gattttcaat attccgcaca ttctgtttcg ctatctagca
     6001 acaggttggg gatatcgctt aggaacgggt tttttgaaaa agatccaggc aaacggtatg
     6061 atggaaagtt tgacattagg cgcgtctatt attggactga tggtcgttgg agcaatgact
     6121 gccacgatga tcaccatcaa tattccttta aaaatcggtt caggagaaaa tgctgtgact
     6181 gttcaaagta tttttgatga tatcgttcca aatatcttat gtcttggagc atttggcata
     6241 gtgttttatc tattgaagaa agaagtgaaa ccattaacga tcttgatcgg actagctttc
     6301 tttggaattt ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga aaatgtttac
     6361 gatgcaagat tatatctatg aagaaaagga agtactaagt acgatcttga aaaagaatga
     6421 tttttcgaca agagaacata tgaaaaaaac agtgaattta ctgatcttag caacgggttc
     6481 atcttataac gcttgtttag cagcgaagcc agcattggaa tcttatggtg atcttactgt
     6541 cgatattcaa gaaccttttt attttttcaa ttatggaaaa ctttctcctt caatcgatac
     6601 tgtgattgct gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag taaaaatgat
     6661 acaaaaacaa gggttacctg ttgttgctat cacaaatgac gtgcaaagtc cacttgcttt
     6721 agaagctgca caaattattg atttaggtgc cggggtagaa tcagtaggct tcgttacaaa
     6781 aggctattct gcgactgttt tacagttact actcttaggg ataggaattg gaataagtaa
     6841 aaataaaatc agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca aaataatcaa
     6901 ccatttacca gcgattattc aaaaaacgga agaatttttt gatggatatc aaagtttatt
     6961 tcgtttagca cagcgtttcg tcaccattgg atatggtcca aactggggaa cagctaaaga
     7021 agcagagacg aaattgactg aaacaatccg cgttccgtca caaggatttg agttagaagc
     7081 ttatatgcac ggcccatacc tggaggcaga tgcttcgcat ttgctattct ttattgaagg
     7141 ggaaagtgta aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc ggtacgttgg
     7201 agagactctc accattacca caaaaaaagc aagaaacgag aaaacacttg gattagcaat
     7261 agaatgtgat gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac tattcgctta
     7321 taaaaccgca gtagctaaag gtattgactt gaacaaaaag atttttgaag actttgatac
     7381 agtcttgaaa agtaaattat aagaggtgga aatcatgctg aaattcaatg aagaagaaca
     7441 aatcaaagca ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa tcatcgataa
     7501 actttatgcg gaaaaatttg acgctgtcta ttatctagga atcggtggaa cgtatgcttc
     7561 ctcgatgcaa gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct ttgtacagca
     7621 tgctgcagaa tattacacta ctggaaacaa aagattaaca aaagattcta tcgttatttt
     7681 gtcctctgtt accggtacaa ctcaagaagt agtgaaagca gtagaacaaa tcaagaaagt
     7741 aggagcgaca ctgattggat ttatcgataa agcgaacagt aagttgtctc agctatgcga
     7801 ttttgtggtc acttatccag ctcctggaac agaacaaatc aaatttttta tggcagcaga
     7861 tcgattgatg tatttgaatg gagaattcga agcttattca gaatactatg aacagctcga
     7921 acgttattta ccaacagggt tagtggaagc agagaaaaaa gcagatgcat ttggcttatc
     7981 atttgcagaa aaacatcgac gtgatagtat gcattatttt attggagcag ggaatcaatg
     8041 gggagctgtt tattcatatg ccatgtgtta ctgggaagaa caaagctggc ttccatcgaa
     8101 atctatccat gctgccgagt ttcttcacgg gacgttagag atcgtagaag aaacaacccc
     8161 agtcacatta tttttaggcg aagatgaaca gagagtgtta tctgaacgtg tggctaaact
     8221 tttgccaaaa atttgcagca attatacatt gatagacaca aaagactatc cagtagaagg
     8281 tatcagtgaa aaatatcgcg gacgtgtttt gtctttctta ttgatgcatg tagtgacaca
     8341 acgcatcgat gctcatgtgg agcagttgaa ctgccatcca ttagagatac gccgctacta
     8401 tcgtcagttt gattattagg atagactgga taaaaagaat ataaaaaata aagagagcag
     8461 cgataactgc tccctttatt tttttaaaat attggcatca attacgttat aaaacacatt
     8521 ttttgcatta tctacagttc aacttataat ctgtttaatg ggacatgtgg gtgccaacca
     8581 ttttttgtta tgcaatagta ctatttagtt gttgcatgga tccgtagtaa aagtccaagt
     8641 acaacgagtt gcgccagttt acatagcgtt ttgtatccag aatgttgact aaaacaaaag
     8701 ttcctatgtc aaccgtcaac agagacaatc tgtccgttgc aggacctgac agatttagaa
     8761 atgttcaatc tgtctcttat gtattttaaa tcaatactca ctaggagaag tttcctaacc
     8821 tcctaatagt aatgctggta gtaacattgt caaaactgct tcctcttagg aacaagcaaa
     8881 gcataacgag tgtaaaaata aaatgtagac tattgtaaca tatcattgat tatttcaagt
     8941 aaaaacgata aaagaagtaa acaaacactt aaaaacaatg gaaattagtc ccaactttaa
     9001 gaaatgtata ctataatgga aattatcaat tatttatttg gaggaatatc catgaaacaa
     9061 aaaatcgcag ttcttggtcc tggatcttgg ggcactgctt tagcacaaac attggcagaa
     9121 aacgggcatg atgtccgtat ttggggaaat gtacctgaac agattgatga aatcaataag
     9181 tatcatacaa atcagcattt tttacctgat ttgacgatcc cagaatcgat tatcggatat
     9241 aaagaattgg cagaagcagt ggataacgca gatgctattt tatttgttgt tccaacaaaa
     9301 gcgattcgtt cagtcgcaca ggaacttatt ggtaaaatga atactaagcc aatcattata
     9361 catgcaagta aaggactaga acaagatacg cataaacgaa tctcagaagt tttggctgaa
     9421 gaaattccag aagaaaaaag acaggcaatt gttgtattat ctggtcctag ccatgctgag
     9481 gaagtggcag tacatgatat cacaacaatt acagctgcaa gtattgatca aaaagcagca
     9541 gcctatgtac aaaaactgtt catgaatgat tatttcagga tttatacaaa taatgatgtg
     9601 attggcgtag aaactggtgc agcactgaaa aatatcattg caattggtgc cggcgctatc
     9661 catggattag gattcggaga tgatgcaaaa gcagcaatca tgactcgagg attggcagaa
     9721 atcagccgtc ttggtgtcgc gatgggtgcc aatcctctga catttatcgg tttgagtggt
     9781 gtgggggact tgatcgttac ttgtacaagt gttcattctc gcaattggcg tgccggtaac
     9841 ttgttaggac agggacataa gctggaagaa gttctagaaa acatgggaat ggtcgttgag
     9901 ggtgtggcaa cgacaaaagc agcagtagag ctagctcaac agctaggtgt tgagatgcca
     9961 attactcaaa caatctataa tgttttgtat aatggagaag atatcaaaaa agcagcaaaa
    10021 gaaatcatgt tacgtgacgg aaaaatggag aatgaatttt ctcttcgtta aatagatgat
    10081 caatagttaa tc
//
LOCUS       DAYYAR010000098         9916 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000098 DAYYAR010000000
VERSION     DAYYAR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.099"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..589)
                     /locus_tag="ACHAT0_002105"
     CDS             complement(77..589)
                     /locus_tag="ACHAT0_002105"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8452032.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(603..2900)
                     /gene="recJ"
                     /locus_tag="ACHAT0_002106"
     CDS             complement(603..2900)
                     /gene="recJ"
                     /locus_tag="ACHAT0_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8452033.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3042..3485)
                     /locus_tag="ACHAT0_002107"
     CDS             complement(3042..3485)
                     /locus_tag="ACHAT0_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8452034.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3486..4274)
                     /locus_tag="ACHAT0_002108"
     CDS             complement(3486..4274)
                     /locus_tag="ACHAT0_002108"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8452035.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4271..5212)
                     /gene="rnz"
                     /locus_tag="ACHAT0_002109"
     CDS             complement(4271..5212)
                     /gene="rnz"
                     /locus_tag="ACHAT0_002109"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8452036.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5389..5976)
                     /locus_tag="ACHAT0_002110"
     CDS             complement(5389..5976)
                     /locus_tag="ACHAT0_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8452037.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6197..7510)
                     /gene="obgE"
                     /locus_tag="ACHAT0_002111"
     CDS             complement(6197..7510)
                     /gene="obgE"
                     /locus_tag="ACHAT0_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8452038.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7798..8808
                     /gene="gap"
                     /locus_tag="ACHAT0_002112"
     CDS             7798..8808
                     /gene="gap"
                     /locus_tag="ACHAT0_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8452039.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8893..9828)
                     /locus_tag="ACHAT0_002113"
     CDS             complement(8893..9828)
                     /locus_tag="ACHAT0_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8452040.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2727 a         2270 c         1715 g         3204 t
ORIGIN      
        1 agagaatctg acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat
       61 agcttctttt ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt
      121 cgcgtccatg gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta
      181 attgttcgat cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac
      241 aaatcagcac acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc
      301 catattctaa tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga
      361 ctggggcaaa accgactcct aactcataag caacaggaca tcctacgatg aatccacggg
      421 cttctggccc aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag
      481 tagcttcccg ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc
      541 cttttgacgg atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc
      601 tcctaggttg ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca
      661 actttgattt tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct
      721 gggctagcga ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac
      781 acctgtatca taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt
      841 attttatgac gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac
      901 tgttctcttg tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa
      961 acacgattga atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa
     1021 tccataacaa ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt
     1081 ccttcgaaaa caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa
     1141 tcaaatgcta aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc
     1201 cgccaatcaa atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc
     1261 ttacgcccgt tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc
     1321 agttcttctt cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg
     1381 gaaaggacaa atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg
     1441 acttgtcgaa ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc
     1501 agctcatcaa ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac
     1561 tcaggccctc tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt
     1621 agtatagtga cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag
     1681 taagtaaata aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta
     1741 cgtccagatc ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc
     1801 gtttcattca ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt
     1861 accaatagat ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc
     1921 tctacgattg cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct
     1981 tcctcttcat caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg
     2041 attgcattta gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt
     2101 tttacgccgc tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca
     2161 tcgattccta atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc
     2221 gttcctatcg ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct
     2281 actttgaatg ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga
     2341 cgagggtgaa caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta
     2401 atgatcacgt ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg
     2461 ttatcaacag tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg
     2521 tttggtccat acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt
     2581 aattccagtg tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg
     2641 taaacgagga ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc
     2701 atatcgtgta tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg
     2761 atatcctttt cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat
     2821 ttcagttcac ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa
     2881 tcaaaatttg cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca
     2941 gcaaaaaaaa gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc
     3001 aggacctgat cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag
     3061 ctttgtttgg gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg
     3121 gttttcgctt gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac
     3181 tcgcgttgct gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct
     3241 gacatttcct gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca
     3301 gtcaaaaata caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc
     3361 gcagaaaagg aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa
     3421 agaacaataa tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc
     3481 ttcacctact ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc
     3541 gtataaaatt ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt
     3601 ctcatattgc ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact
     3661 ttttccaaat agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct
     3721 ggattcactg tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag
     3781 aagccaagga ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt
     3841 ccagccatag aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca
     3901 aatttttgtg tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt
     3961 ccaaggtcga atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata
     4021 tcgacgcgcc cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg
     4081 ctgacgtcta ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg
     4141 acaccttcta caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct
     4201 gcttcataac agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta
     4261 tcttctaaat tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt
     4321 tcataatcgt cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt
     4381 ttcctaaata tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg
     4441 caacttcagc agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat
     4501 ctttattaaa tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat
     4561 tatgtgtttt tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta
     4621 cgaaatcttt tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca
     4681 gtttgccgta cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt
     4741 ctcctttatg atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt
     4801 tttttgtata aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa
     4861 tgaatttcag cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt
     4921 cctctatccc ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc
     4981 tgctgatgag cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt
     5041 taccgatttt tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac
     5101 agtcgaacaa ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat
     5161 ttctatgttt tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc
     5221 tgactttctt taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag
     5281 tcgagaatgg gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt
     5341 actataaaaa cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga
     5401 tccagaactt tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc
     5461 agcttctttc gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc
     5521 ataatccaag cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg
     5581 attgtcctct tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc
     5641 tacaaagtcg aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat
     5701 cattcctccg gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa
     5761 cattccgatt aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc
     5821 tattttgaat tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt
     5881 aatcgaacga acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc
     5941 tacttgaaaa tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc
     6001 ttgttattta ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa
     6061 taaaataggg ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct
     6121 tgaagctgat cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt
     6181 gtttttattt gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt
     6241 cgccatcttt cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac
     6301 gagcaaaacg catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga
     6361 tcttctcacc agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac
     6421 cttctggatt gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga
     6481 attctggcgt tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc
     6541 gagtcactcc agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct
     6601 tgttcagctg ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt
     6661 ttgccacgat gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt
     6721 tgatggcaag atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat
     6781 gtaaaattac tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt
     6841 gcgaagctcc ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg
     6901 tagtaaccat tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac
     6961 gagcagatga aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc
     7021 caacgtctgc caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt
     7081 tttcagcaat ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac
     7141 cgcgtccacc ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag
     7201 caccagtctc cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg
     7261 aaccacgacc gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc
     7321 gattgaaccg aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc
     7381 cgccacgacc accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa
     7441 atgcaaccat tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa
     7501 acatggacat aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca
     7561 caataaacaa aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc
     7621 aaaaagattg tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct
     7681 gctaccaagt ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa
     7741 ttgtattaat tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg
     7801 acagtaaaag taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc
     7861 aaagaagtct cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg
     7921 ttagcacaat tattgcaatt tgactctact catggtactt atcctggcac agttactgca
     7981 acagaagact caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca
     8041 agcaaaatcc cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt
     8101 tatacatctg aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt
     8161 tctgccccag ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat
     8221 gaaaacgaca aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct
     8281 tatttcttaa ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac
     8341 acttccacac aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt
     8401 tcagctgctg acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt
     8461 atccctgagc tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt
     8521 tcattaactg aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa
     8581 gcaatcaaaa aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt
     8641 tctagcgatg tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta
     8701 acaactgctg gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga
     8761 tttacttgcc aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca
     8821 atcgataaaa gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac
     8881 ttttaaactc gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt
     8941 ttcaggatct gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt
     9001 tggtcccttc gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc
     9061 gtctccatgt ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc
     9121 ttcaccgaaa aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg
     9181 aaatgctgga agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga
     9241 ataaccgcaa tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat
     9301 cataactgtt gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt
     9361 gttttctagc actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc
     9421 aatgtaatcc ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc
     9481 catatcatga aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg
     9541 agccacaaga gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt
     9601 cagaccgtct tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt
     9661 atgtgcatcc gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc
     9721 tttctcagaa ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac
     9781 aatcagagct aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc
     9841 tcatttctct tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa
     9901 tgttcatttt tataac
//
LOCUS       DAYYAR010000099         9878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000099 DAYYAR010000000
VERSION     DAYYAR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.100"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1483)
                     /locus_tag="ACHAT0_002114"
     CDS             complement(<1..1483)
                     /locus_tag="ACHAT0_002114"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8452041.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKR"
     gene            complement(1805..3157)
                     /gene="murF"
                     /locus_tag="ACHAT0_002115"
     CDS             complement(1805..3157)
                     /gene="murF"
                     /locus_tag="ACHAT0_002115"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8452042.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3310..4386)
                     /locus_tag="ACHAT0_002116"
     CDS             complement(3310..4386)
                     /locus_tag="ACHAT0_002116"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8452043.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4582..6327
                     /locus_tag="ACHAT0_002117"
     CDS             4582..6327
                     /locus_tag="ACHAT0_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8452044.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6607..7044)
                     /locus_tag="ACHAT0_002118"
     CDS             complement(6607..7044)
                     /locus_tag="ACHAT0_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8452045.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7028..8401)
                     /locus_tag="ACHAT0_002119"
     CDS             complement(7028..8401)
                     /locus_tag="ACHAT0_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452046.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8723..9211)
                     /locus_tag="ACHAT0_002120"
     CDS             complement(8723..9211)
                     /locus_tag="ACHAT0_002120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8452047.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9233..9835)
                     /locus_tag="ACHAT0_002121"
     CDS             complement(9233..9835)
                     /locus_tag="ACHAT0_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8452048.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2881 a         2112 c         1790 g         3095 t
ORIGIN      
        1 cgcgtttttt atcattgtga cgtttaccgc catctttttg gctgttatta tttttgctat
       61 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
      121 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
      181 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
      241 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtatttat
      301 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
      361 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
      421 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
      481 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
      541 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
      601 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
      661 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
      721 attcgtctac acggcgtttt gtacgtccga agacgattgt taattcaggt gtttgaacat
      781 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
      841 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
      901 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
      961 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
     1021 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
     1081 taatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
     1141 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
     1201 cgcgccctaa acgatataat tcttcttgtg tttggatagc taattcacgt gttggtgcga
     1261 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
     1321 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
     1381 ctagtggaat cgtttctgct tggataggcg ttgcttcttc aaaaccagcg cgttcgactg
     1441 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
     1501 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
     1561 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
     1621 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
     1681 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
     1741 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
     1801 atttttattt catttgctga agttgttcta ccacttcaaa taagcccatg ctgttgctgc
     1861 cttttagcat aatactatct gttgcttcaa gattttgttt gatagcatca atcaatttat
     1921 tcttttcttt ttccaaatag aacacaggta gttgggggta tttttggttc aattcttctt
     1981 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
     2041 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
     2101 acacagcgat tctgcgtcct tttgttggca gatttttaaa agtatctaat accagtccca
     2161 tggctgttgg atttgcattg tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
     2221 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
     2281 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
     2341 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
     2401 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
     2461 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
     2521 cgtctgatac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
     2581 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
     2641 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaaa acgatctttt
     2701 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
     2761 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
     2821 tggttttacc attgcttcct gtaattgcaa cgacttctgc gcctactttt tccaagtaat
     2881 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatttt
     2941 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
     3001 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
     3061 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
     3121 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
     3181 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
     3241 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcctcttctt
     3301 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
     3361 taactggatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
     3421 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
     3481 atttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
     3541 agctaacttc gcgtactctt gcgctttttg ataaacttct tctggcactt cggctggaat
     3601 ctgcatttcg attttattat tgatatattt tgcttcataa tcatagaatg ctacgtcttt
     3661 tacgacttca ccaggcaaag tcgtccgaac gtcttcattt cctaatacag caacttcgat
     3721 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
     3781 taaagcattt tgcagctctt ctcggttttc tgcttttgta atgccgacac tagaacccat
     3841 attcgccggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
     3901 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
     3961 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
     4021 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
     4081 cccgtttggc ccatgtaaaa ctggaaaaac gatggctcct tcttctttga tttcgcccgg
     4141 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
     4201 caagacatct ttgctggcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
     4261 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
     4321 aaatgcggaa agaatggaca cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
     4381 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
     4441 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagccagctt ctaaaagaac
     4501 gagcgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
     4561 taaagaaaag gagggatgat gatggcgcaa acaaatccac gcagccgctt ggcagaaatc
     4621 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
     4681 caggtaaaaa aagcctttga agaactaggc cccaccttca tcaagatagg acagatgctt
     4741 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
     4801 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
     4861 tcattatcag atatattcga tgatttttca aaatcgcctt ttgcctctgc ttctatgggg
     4921 caggctcatc gtgccaagct gaagaacggt gacgcggtag tcgttaagat ccaacaccct
     4981 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
     5041 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
     5101 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
     5161 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
     5221 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga atcatttaat gaacgccgaa
     5281 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
     5341 aagcttttag ttgagaactt tatgaaacag gtgtttgatg atggtttttt ccatgctgat
     5401 cctcatccag ggaatctgct attccatgta ctaacaaaag aagaacagac acaagctagc
     5461 cgaaaaactg aaacagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
     5521 tctgcagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
     5581 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
     5641 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
     5701 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
     5761 gatgaaatca acttgcaaga agtattcaca catgtcgtga cgatttgtca tcaaaataat
     5821 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg
     5881 atccgcgtcc tcgatcctga agtttccctc atggaagtcg cttcgccttt tgctcaacat
     5941 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
     6001 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
     6061 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
     6121 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
     6181 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
     6241 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
     6301 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
     6361 aaataagaag gggttttaca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
     6421 ttcgaagagg ctacttctga aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc
     6481 cagtaataag aacaatcaaa aagggaacat ggaacacatt tatcctgttt ccctgttccc
     6541 tttttgatgt attcgagcat aaataaaata ccttcgttac ctactatctt ctgtacttgg
     6601 taaaccttag tcaattgcct tttttaacgc ttgacgaaaa gacatatgat ctttctccgt
     6661 aaagggtttt ggaccctttg tccttatgcc agcttttctt aactttccac tatgataacg
     6721 ttgttctaga agcgtgccga tattcgcttc gtcaaattct tttccagagt gatggaacac
     6781 tcgcacaccc ttcggcaata caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc
     6841 attcttctta accataccga cgattttata atccgcacta tcagctccgc ggtctacgta
     6901 gacgatttgg acatgattag cgtattcttt ggtcgtataa tggtccacac tggtcacgat
     6961 aaccacgtca agaccatagg attctcctaa ttttatgaca tcttctttta ctggagaacc
     7021 atctccatca atgaatagcc tcattctttt tcgccttctt ccataaatga taaacagtta
     7081 ctaatccgct cagtaaaaga ccaacattga tccattttgc caagataagc agttgtacac
     7141 tttctatcgg cactgattca tggttcagcg tgatcaataa tagatggatt ggctctccca
     7201 tttcttttgc taaaacaaac aaaagcgtca tcaataaaaa ctggtggatc acactccctt
     7261 tattttgagt agtatacttg acaacgatcc ggcgatccat tggaatagct gtatcagttt
     7321 gataatattc cttatttagc cagctgcctg ttactacgtg ccagaaacta agcagaaaga
     7381 cacctatcgc aaaaccattt ggaatcaaga ggaccaataa agaagaactc agtccaagca
     7441 attgcatagc caatggagac catgttaaac gtttcaaaat ccatggtccg actatttttg
     7501 ctgcaatcaa gcctgctgca taaggcaaat acataaaaat cgaaagtttt tctaatccgt
     7561 acagtatgcc aacataaaaa ctgctaaata aaaaaaggaa attccctagc attccattta
     7621 gaaaagtaaa cagatttagc cagcttggca acttccattc cttcttctgg tgtcctaaaa
     7681 caaacacgaa caagatgaac agcagcaaga aaccataaat agcaagatca aaaagccttt
     7741 catcgaataa caatcgggca tttctcaaaa gaaacaataa gccaaagaat atcaaaaata
     7801 aaacaagctc tcgcgtagct accagatttc ttttgatgtc tttgaaatcc agttcgtaac
     7861 gcggataatg actaaccgta tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc
     7921 ttgctgcaat ctgagtcggt gcaggcaact caatcgcttg atacaggaga aatagccaga
     7981 tagctgcaaa taagtacttt ttcccatcca tattgataaa accttgcgtc ttttcgtgaa
     8041 aattgaccgt ggtattagca ggtacaagcc atgctgcact cagccctagc aaaacacttg
     8101 aaaaataaaa taaaggaaaa taaaatacgc ctaatattcc aagcaatgaa ccagcaccac
     8161 caatcagcag agcgatcatc agcaacgtgt aactatctag accaaatcgt agactgttga
     8221 ctaaaaatag tccagtcatc cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc
     8281 aattatgcga gtctgttagt cccaaaaata aaagataagg aataaaaata accgtgttac
     8341 ttaagataaa gggcgttgtt tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca
     8401 tgacaatggc tccttaaaca gtcttataac taatctgctc ttcgatttta gcattaaagg
     8461 ggaaataaac caattttaca gaatatattc ttgtaaaaaa aaatacagaa agaagcataa
     8521 gcttctttct gcatttttac ggtacttgat tactcaagca gacaagccct tctccacaaa
     8581 agtcttgccc gacacccctg catccggcag gtgataaaga tagaaatcaa ttatcctgtg
     8641 cctccaaaat caattaggag ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt
     8701 tactttaaca cagttcctat gtttatgcga gagctttttt cattttataa tcgaccattt
     8761 gaaatccttg accgatatct gaaataagtt cttctgtaat ctcaaatcct tcatgcttat
     8821 aaacctcgat tgcttgatga ttcccctgat tcactcgcaa aaagatctct ctttcctttg
     8881 tttgcgcagc aagttcttca taccagcgaa aaactgaaga agtcaatcct tgaccacgta
     8941 ctttatttag caaatataat ttacttaaat aaatttttcc atttatctct tcataagcag
     9001 tgtaaccgac agtcttttct tcgcttttta ataaaaagta ggacacacct tctgtcagtt
     9061 ctttttgaat ctgttttttt gactgataag ttttcagcat gtaatccact tgtttttgac
     9121 caatgatcgg cacaaaagct tccggccata cttcttgtat gactgccatc agctcatcca
     9181 gttcattttc tgttactttt attttttcca tacacccacg tcctttttta cattataaat
     9241 gaccttgcgc ttgatggaat aaaaagagca tgatcatgct atacacaccc accgaccatg
     9301 gtttcaagaa aatcacaatc tgcataaatt ccctaaaaga taatttcgtc aaaccagcta
     9361 ctagacagat caagtcgtct ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt
     9421 taatatcttt cgtttttctt gtcagcaatt catcaaattt ctgatacttt tttggcgata
     9481 aaataagctg gacaaaggct acgccatagt aacggacaag caaaaatccg attgcttccc
     9541 caatcactag gccaatacaa ctatataaaa gcccaaatcc attgccaaac atcagcatgc
     9601 ccgctactga ggagatccct cccggcaaaa taggaatgat cacttggatc acttgcaaca
     9661 gaacaaatat catcactgca tacttgccaa attgctggat aaacgcttgc aaagacgtgc
     9721 tcgatcgaaa aatcccatga tgataagcat aaagaatcaa cacaaagaaa aaaaacagac
     9781 caataatggg catgatctga agacattttt gtaaaactga tcctttttta gacatctata
     9841 ttccccacat cttttttcat ttattaaaac tgcctcct
//
LOCUS       DAYYAR010000100         9870 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000100 DAYYAR010000000
VERSION     DAYYAR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.101"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..263)
                     /locus_tag="ACHAT0_002122"
     CDS             complement(18..263)
                     /locus_tag="ACHAT0_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8452049.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(378..956)
                     /locus_tag="ACHAT0_002123"
     CDS             complement(378..956)
                     /locus_tag="ACHAT0_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8452050.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1033..1563)
                     /locus_tag="ACHAT0_002124"
     CDS             complement(1033..1563)
                     /locus_tag="ACHAT0_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8452051.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1567..2427)
                     /gene="rlmB"
                     /locus_tag="ACHAT0_002125"
     CDS             complement(1567..2427)
                     /gene="rlmB"
                     /locus_tag="ACHAT0_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8452052.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2420..2815)
                     /locus_tag="ACHAT0_002126"
     CDS             complement(2420..2815)
                     /locus_tag="ACHAT0_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8452053.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2812..4224)
                     /gene="cysS"
                     /locus_tag="ACHAT0_002127"
     CDS             complement(2812..4224)
                     /gene="cysS"
                     /locus_tag="ACHAT0_002127"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8452054.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4221..4757)
                     /gene="epsC"
                     /locus_tag="ACHAT0_002128"
     CDS             complement(4221..4757)
                     /gene="epsC"
                     /locus_tag="ACHAT0_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8452055.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4843..5082)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5079..6539)
                     /gene="gltX"
                     /locus_tag="ACHAT0_002129"
     CDS             complement(5079..6539)
                     /gene="gltX"
                     /locus_tag="ACHAT0_002129"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8452056.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6709..7866)
                     /locus_tag="ACHAT0_002130"
     CDS             complement(6709..7866)
                     /locus_tag="ACHAT0_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8452057.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7900..9270)
                     /gene="radA"
                     /locus_tag="ACHAT0_002131"
     CDS             complement(7900..9270)
                     /gene="radA"
                     /locus_tag="ACHAT0_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8452058.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9333..9860)
                     /locus_tag="ACHAT0_002132"
     CDS             complement(9333..9860)
                     /locus_tag="ACHAT0_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8452059.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2507 a         2213 c         1784 g         3366 t
ORIGIN      
        1 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
       61 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
      121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
      181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
      241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
      301 taaaattttc aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc
      361 attaagattt ttctaaatta ttcctcatta taaaatttca agtgtttttt tattttgagt
      421 tttactctcc cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca ttcagccatg
      481 tctacaaaat caacgccttg catataataa tgaaaaattt tagcttctat ttttgaaaac
      541 tggatttcgc aattttcgat tgcttctttt aataaaagat gctgaatcgc taaaggttct
      601 tgcaatgtga tctctgctaa aaagtctggt ccttctgtct ctattttttg gtcgattgag
      661 acttcatctt tttgatcacg acgcttcagc gcgttctgct tcctcaacag agaatagatt
      721 tgattgctta taatgctttt ataataactt ccaaagctaa cacctttgct tttttcgtaa
      781 gttctcaatg cctgatataa agcgatcctt gcttcttgtt cccaatcctc agaaacataa
      841 tttcttaaat aataggtgtt tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg
      901 gcagcaaatg cttcttcgtc accattacgt gcagcgcgca ataactcctc agacataaaa
      961 taattcctcc ataaattcgt ttatggagga atcttatcat caatgattca tcgaactgat
     1021 tagaacgata tgttatttat tttttctcat taaatcgtcc aatttttggg acaatttttt
     1081 taactgctct tcattccatg gagaattacg tcggaaatca ataaaatgca tatctttatt
     1141 ttcttgggct attgtttttt ccgttttttt tatcgtctta tataattcat aagcagaagt
     1201 tcgtaatgct ccttgggaaa agatcatcca ctgttctgct aaatcgcttg tcgccactgt
     1261 cacttgtgtc aaacggtcat tcagctgacc agcgattctc tcgatataac tgtcagccgt
     1321 ttcgtcttct ttcgtaaaaa caacatccaa ctggtatttc ttataatttt gttggatgcc
     1381 cggaactagt tgggcatcga agacaacgat gacttccatt ccttcatatt tcgcataatt
     1441 tgataaacga tgtaaaagag cttctcttgc atccgccatt ttgtcctgct ttttaagctg
     1501 gacaagttcc ggccacgaac caatcatgtt gtagccgtct acaatcaaca gctgtttttt
     1561 catggtctac tccccctgac gttgggaaaa agcctgatac ataagcaaac cagcagcaac
     1621 actggcattt aagctctgga cgtgcccggt catcgggatc gtcagcaatt catccatttc
     1681 ttttttcaat cctggactca ttcctttgcc ttcattacca atgactaaag caattgatcc
     1741 tttggcattc catttttgga agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc
     1801 tgtttctttg agctgttgca cagcttgtac caaattcgtc acgcgtgcaa cagggacatg
     1861 ttcgactgcc ccagtggaag tctttgttac gataggtgtg attccaactg ctcggtgttt
     1921 tggtatgatc acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt
     1981 gtgtggatct tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt
     2041 tcgttcaatc agttggtcaa gggataaata ttcataaggt gtaatcgcaa gaacgactcc
     2101 ttgatgcacg ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc atttgactgg
     2161 cacagcttgt tcacgtgcga tttctttcag ttcttctact ttttctcctc gtgaatcttc
     2221 ttgaagaaag agtttattcc ctcgcccttg ttgtagcgct tctactgcgg catggtgccc
     2281 aaagacgaaa ttatcttcta atgcttcttc attttgatca gaaactgact gatttcgtcc
     2341 agtttgcttt ttatttgaat atctattttg ttttttgtct tcaaatccac gtttcgattg
     2401 atgtttgttt gttctttctt tacccatttt tttcaccaac tttctgaata caccagcgga
     2461 tcaattcttc catccgctcg gtttgttttg tcaaatgcag atagcccatg agcgcttcga
     2521 accctgttgc gacccgatac gtcgtaacat cagcattttt tgcgctcgta tggcttttag
     2581 catttcttcc tctgcgataa taaagttctt cttcttctgt caaaagatgt tcttccagca
     2641 tttgctgaat caaaaaggct tgtgcattcg cagaaacata attcgttgcc gcacgatgca
     2701 gcttattggg ttttgtttct ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa
     2761 tcgcatctcc aacataagcc aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc
     2821 tcttctccat cgggttcctt gcggtgtatc ttctaataag atcccttttt ctttcaacaa
     2881 atcacgaatt tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat ttcgttcttc
     2941 gatcaattgt tcaatctctt catctaataa ctcttctgct gtaaataaaa taccgaagat
     3001 cgaaagccat tgcgagaata aagcttctcc ttgtgccaag acagcggcag aaactttcgg
     3061 ctggtctaga taagtgttca gccacttagc aagctcatag accactgtga tcccatttgc
     3121 tgcattaaaa tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc
     3181 gattttatcc gcatcatctg ccaaattctc tgaggcagtt tcttggcgga atttcaaatt
     3241 gtcaaacgta ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg tcgtttcact
     3301 gtagcggatc ggacgacggt attgtgtggt cgccatgaag aaacgcaaga cttgtggatc
     3361 aacgtttttg atcatttcat gtacagtgat gaaattccct agagatttac tcattttttc
     3421 atcgtcttcg ccaatcgtta catagccatt gtgcatccag taattagcga attttttccc
     3481 tgttttcgct tcactttgtg cgatttcatt ttcatgatga ggaaattcta aatcttgccc
     3541 gccaccatgg atatcgatcg tttctcctaa atgttttgtt gccataacgg aacattcaat
     3601 atgccaaccg ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc caggttttgc
     3661 ttgtttccat aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt cccctgtacg
     3721 ctgacttgct ccgacttcca gttcatcgat ggattggtca cttaatttcc cataattttc
     3781 aaatttacgg gtacggtaat acacatcacc tgaagattca taagcatagc ctttttcaac
     3841 caacacttga ataaatgcta aaatatctgg catatgatcc atgactcttg gatgcagtgt
     3901 agcaggctcg acattcaacg ctttcgtatc ttcttcaaat gctcggatga aacgatcagc
     3961 aacttctggt gctgtaatac ctaattcgtt tgctgtacgg atgattttat cgtctacatc
     4021 cgtgaaattc gatacgaaat cgactttata gcctcgatat tcaaaatagc gtcgaattgt
     4081 atcgaaagca atggtgctac gagcatttcc gatatggata taattataca cagtcggtcc
     4141 gcagacatac ataccgacat gtcccttttg tatagggatg aattcttctt tttttctggt
     4201 caatgtattg tagattttta tcattctgat ctcctcactt tttctccttt gattcgaacg
     4261 acttttgctg gtactcctac cgctgtagcg tcgtctggaa catctttcaa cacaacggca
     4321 cctgcaccga ttttggcgtt ttttccgatc gttaccggtc ctaatatctg cgcattcgca
     4381 gagagcatgg ctccttgttt aacagttggg tgtcttttac ccgtttcttt tcctgtgccg
     4441 cctaatgtta cgccatggaa taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg
     4501 acgattccca ttccatggtc gatgaacacg ccagttccaa tcgttgctcc tggatggatc
     4561 tcaacaccag ttaggaaacg ccagaattga gcattgatct tcgccaataa aaagagccgg
     4621 tgctgatata aaaaatgaga gaaacgatgc caaaacaagg catgaaaacc agggtatgtc
     4681 aaaacgatct cagctgtcga acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct
     4741 cgggtaaacc aactcatgac tttccactcc tttcgtaaga attttatgta ggaaatcatc
     4801 ttcttataaa gaaaagaagc gccttttaaa taagtttcct tatcaaaaga cgctttccgc
     4861 gtggttccac ttttcttcat aatcaatgat tatctctgac agctaacgcc tgttatacgt
     4921 tcttggctac tttgtttcct ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc
     4981 actcccagag gcatttcggc aaccattcat tgataccttg caccacccgg tatctctctt
     5041 gtaatgatca gctgcttact tttttctgtt catcgtgttt agttcagtac ttgatttaaa
     5101 tgtgctaatg atttttcacg accaagcaat tcgatcgttt caggcaattc tggaccatgc
     5161 atttgtcctg atacagctac acggattggc atgaaaagat tttttccttt tacgccagtt
     5221 tctttttgaa cagctttgat ggcagctttg acagttggag catctactac ttccatttgt
     5281 tctagctgtg ctttgaatgc gtggagaaca gtaggaactg tttcaccagc aagtacttct
     5341 tttgctgctt cattcaatac aggatgttca ttgaaaaata gcgtagacag atcaacgatt
     5401 tctgcagcat agctcatttg aggttgatac aggctaacga tcttcttcac ccattcgatt
     5461 gtcttttcat ctgggttttc tgctacacgt ccatcagcga ttagataagg cagacacatg
     5521 tcagtaaatg catctagatc cattgctttg atgtaatggt tgtttaccca ttgcagtttt
     5581 ttcgcatcga aagctgcggg tgatttactt aggcgttttg gatcgaacat tttgatcaat
     5641 tcttggtgtt cgaagatttc gtcttcgccc acaggtgacc agcccaataa tgcaataaag
     5701 ttgaacattg catctggtag atagcctaat tcgcgatatt gttcgataaa ttgcaagatt
     5761 gattcgtcac gtttgcttag ttttttacct gtttcactgt tgatgatcaa tgtcatatgt
     5821 ccgaaggttg gtggtgtcca gccaaatgct tcatagatca tcagttgttt tggtgtattg
     5881 gcaatatggt catctccacg taaaacgtga gtgattttca tcaaatgatc atcgaccgca
     5941 acagcaaaat tataagttgg cataccatcg cgtttttgta tgacaaagtc gccaccgata
     6001 ttatcggatt cgaacgaaat agcgcctttc accatatctg taaatgcgta ttctgtattg
     6061 cgaggcacac ggaaacgtac aacagattct agaccttgtg cttctttttc tgcttgttct
     6121 tcaggtgtca agttcgcaca tgttcctgcg taatgaggca tttctcctct tgcgcgttgt
     6181 gcttcccgtt ctgcttctaa ttcttcttct gtgcaatagc atttgtatgc tcgattgcta
     6241 gcaagcagct gatcaatcag cggttgatag atttctttac gttcagattg acggtaaggg
     6301 ccgtattctc caggattttc aggagattca tcccaattca ttcccaacca agcaaggttt
     6361 tccaactggc ttttttcgcc atcttcgata ttacgttttt ggtcagtatc ctcaatacga
     6421 ataataaagt ctccatcatt gtggcgggca aacaaataat tgaataatgc agttcttgca
     6481 tttccaatat gcaggtgccc agttggactt ggtgcatagc gtacgcgtac tttcgtcatt
     6541 ttcttcctct tctttcaaac tagactttga ctaacagcaa aaattccgtc agttacggct
     6601 tctcaaatat catttattta aagccactca gcacaaattt taccttttct aaggtaaata
     6661 gtaaagccaa ctggttgaat ttccttacca gttggctgaa tttctttttt attttgattg
     6721 tttagtcttt ttctctgtct cttttttatt gtcgattcct cgtcctgcat gtgcaggttt
     6781 agcaaagatc atgcgtccag cagctgtttg aagggcactt gttacaacga cttcgatacg
     6841 ttcattcatg taatgctgtc catcttctac gacaaccatc gtgccgtcat caaggtaggc
     6901 tactccttgt tggcgttctg tcccggcttt tactactaga acattcatcg tttcaccagg
     6961 gatgacgact ggttttacgg catttgccaa cgcattgata ttaagaacag gtacattttg
     7021 gaattcagag actttattca agttatagtc attggttaca atgacaccat ctaaaagctt
     7081 agccaatttg atcaatttgc tgtctacttc actgatatct tcaaagtctc catcgtacat
     7141 ttcgacagat ataccctctt ctttttgcaa ggcatttagg atatctaatc cgcgacgacc
     7201 acgcacacgt ttcaaactat cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa
     7261 attagggatc atcaaggttc cttctaagaa tccagtcttt gtaatatcat aaatccgacc
     7321 gtcaatgatc acgcttgtat ccaagatttt atatttatgg aaattttctc ctgattttcg
     7381 ttctaacagt tgactctcaa cttgactagc tgtttcttgc tcgatttttt tgcttcgtga
     7441 accaaaaact ttcttccatt cttcgattcg tgtcgttccc attctcaatc ccagatagcc
     7501 taagatgatc atcagtaaaa tcggcaatac actattgacg aatggaattt ccatattgta
     7561 aagaggggtc gagatcacaa cacctaaagt caatccgatg atcgcaccga tcgcaccgaa
     7621 gagaagataa gtcaagctga cctcacttag tttttgttct acttgtttga taatagctga
     7681 gatatgtttg gccaacagca aagataataa aaagaaaata agtgcgccta acaggctgtt
     7741 ggtaaatgta ttattcagcc atccgtttga ctgttgaccg attgcctgcc aagctgaggg
     7801 taaaaatgaa atacccaagc ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt
     7861 ttgcatcgtt tttcctccgt ttcgacaatc atttattagt tagcggaaca cacgcttcaa
     7921 cgtttcagcc aaagttgcta ctccaactat ctcgattcct tttggcgctt cccagttacc
     7981 taagttgttt ttaggtaggt atacttttgt gaagccgagt ttttggactt ctttgactcg
     8041 ttgttcaata tgactgacac gtctgatttc tccagtcagt ccaatttcac cgatgaaaca
     8101 ttccgttggc tgtgttcctt tttctttata gcttgaagca atactgacag cgatagctaa
     8161 atcaatcgct ggttcattta attttacccc acctgcagct tttaagtaag catcttgatt
     8221 ctgaagaagc aacccagctc ttttttccag cactgccatg atcagagata cccgattgaa
     8281 atcaagacct gttgttgttc gtttggcatt tccgaacata gtaggtgtga ctaatgcttg
     8341 gatctctacc aaaattggac gtgtcccttc cattgccaca acgatagcag agccagttgc
     8401 accgtctagg cgttcttcta agaaaatctg tgaaggatta gcgacttctt ccaatccatg
     8461 ctcatgcatt tcaaaaatcc caatctcatt cgttgagcca aatcggtttt ttactgctcg
     8521 caaaatccga aaactatgat gttgttcacc ttcaaaatat aacaccgtat ccaccatatg
     8581 ttctaacatt cttgggccgg cgatcgagcc ttctttggta acatgaccga cgataaaaat
     8641 ggctatccca tttgttttag cgattttgag tagttctgca gtggtttctc ttacttggct
     8701 gacacttcct gccacacttg tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat
     8761 aacataatca ggggagatat gttcgatggc acgactgatt tcgttcatat ctgtttcagc
     8821 gtatagataa aattctgtgt tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc
     8881 tgcgctttct tcgccggata catataagac ttttccgcct atggctgcta attgctgaga
     8941 aacttgtaac agcaaagtgg atttcccgat acctggatcg ccaccaatca agaccataga
     9001 ccctggcacc acacctccgc ctaacacacg gttcagttct tctaattttg ttttgatccg
     9061 aggttctttt ttagggacga cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa
     9121 acttgtccga gttctgcggt ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca
     9181 ttttccgcaa tttgggcagc gtcccaagaa cttaggcgac acatacccgc aattttggca
     9241 aacaaattgt acttttgatt tttttgccat tgtaccgcct ccttttatcc taatcatgag
     9301 acattaaatc aagcttaagt atctttaacg ttttatttac tggaagaacc aaatccgcct
     9361 tgacgttgag cagtcgtttg atcttgatct gcttttaaaa acggcaaaaa gatcccttgg
     9421 ccgatacgct cgcctttttt gatcactaca tccttgaaac caaagttggt gaattggaac
     9481 atgatatggc cttcatttgc ctcattattg taataatcac tgtcaatcac acctactccg
     9541 tttgtcaaca ctaaaaaccg cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat
     9601 tcatcttctc ccatgtaagc tttgattcca gtaggcacga gggttggttt caattgtttt
     9661 tcattttctg atacatcttt tttcaatcca tctgcgactg tttccaaccc ctttttccag
     9721 cctgctttcc agatacttgg aatcacgacc tcttctgctg cttcaaaatc atagccagca
     9781 gcatgatgtg tagcgcgctg cggtagattg atattttgat ctttatattt agaaataact
     9841 tcaaatcctc tttttctcac cggttttcct
//
LOCUS       DAYYAR010000101         9840 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000101 DAYYAR010000000
VERSION     DAYYAR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.102"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2198)
                     /locus_tag="ACHAT0_002133"
     CDS             complement(78..2198)
                     /locus_tag="ACHAT0_002133"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8452060.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACHAT0_002134"
     CDS             2428..3606
                     /locus_tag="ACHAT0_002134"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8452061.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACHAT0_002135"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACHAT0_002135"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8452062.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACHAT0_002136"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACHAT0_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8452063.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACHAT0_002137"
     CDS             complement(4959..6212)
                     /locus_tag="ACHAT0_002137"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8452064.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACHAT0_002138"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACHAT0_002138"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8452065.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACHAT0_002139"
     CDS             7789..9135
                     /locus_tag="ACHAT0_002139"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8452066.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACHAT0_002140"
     CDS             complement(9443..9829)
                     /locus_tag="ACHAT0_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8452067.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3141 a         1792 c         2012 g         2895 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
//
LOCUS       DAYYAR010000102         9807 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000102 DAYYAR010000000
VERSION     DAYYAR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.103"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(192..1040)
                     /locus_tag="ACHAT0_002141"
     CDS             complement(192..1040)
                     /locus_tag="ACHAT0_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8452068.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1204..2106
                     /gene="gnd"
                     /locus_tag="ACHAT0_002142"
     CDS             1204..2106
                     /gene="gnd"
                     /locus_tag="ACHAT0_002142"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8452069.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2108..3109
                     /locus_tag="ACHAT0_002143"
     CDS             2108..3109
                     /locus_tag="ACHAT0_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8452070.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFNVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMCEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3106..3390
                     /locus_tag="ACHAT0_002144"
     CDS             3106..3390
                     /locus_tag="ACHAT0_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8452071.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3423..4220
                     /locus_tag="ACHAT0_002145"
     CDS             3423..4220
                     /locus_tag="ACHAT0_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8452072.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLSSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4234..5067
                     /locus_tag="ACHAT0_002146"
     CDS             4234..5067
                     /locus_tag="ACHAT0_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8452073.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5085..5942
                     /locus_tag="ACHAT0_002147"
     CDS             5085..5942
                     /locus_tag="ACHAT0_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8452074.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5964..6473
                     /locus_tag="ACHAT0_002148"
     CDS             5964..6473
                     /locus_tag="ACHAT0_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8452075.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6510..6911
                     /locus_tag="ACHAT0_002149"
     CDS             6510..6911
                     /locus_tag="ACHAT0_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452076.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7023..7376
                     /locus_tag="ACHAT0_002150"
     CDS             7023..7376
                     /locus_tag="ACHAT0_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452077.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7410..7853
                     /locus_tag="ACHAT0_002151"
     CDS             7410..7853
                     /locus_tag="ACHAT0_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8452078.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7870..8955
                     /gene="uxuA"
                     /locus_tag="ACHAT0_002152"
     CDS             7870..8955
                     /gene="uxuA"
                     /locus_tag="ACHAT0_002152"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8452079.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8948..9661
                     /locus_tag="ACHAT0_002153"
     CDS             8948..9661
                     /locus_tag="ACHAT0_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8452080.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3257 a         1553 c         2166 g         2831 t
ORIGIN      
        1 ctttgttatc gcatctaaca agtaactcga ataataccaa tacaaccttc tccaaagatg
       61 cgatgacata cgttctaacc caccgactat tgatgttctg taccaccctt gcagaaccaa
      121 aaaagccact accaaatatt tggtagtggc tttttttgtt ttatggattt ttcgcttttt
      181 ctgttatggt gctattcttt gatttcagaa atagtatgtc gaactttagc gatagaacga
      241 cgtgattctt tttcgttgta aaacatcaaa atcacatata gagagtccaa aatgcttagt
      301 tgagaaatac gggaagctaa agcttcagat cggaattctg tttcttcaga aatagaaata
      361 aaaacgacat cgccaagttt agcaagggaa gaattagctt ggctagtaat aacgattgtt
      421 tttgctccag tttttcttac ggtttcagca atatggattg tttcttttga ttgccctgtg
      481 tgagaaatca aaacagcaca gtctttttcc gtcaataaag aagcttccat caattgaata
      541 tggtaatccg tgctgtgaga agttgcgata ggggaacgca aaaatttatg ataagcgtca
      601 gttgctacaa tctctgaacc gccaacaccg aaaaaataga cacgtttaga ttcattgata
      661 atagtagccg ctttttttag atccttttgt tgtaacaact tttttgtgtc atttaaagtt
      721 gaaatactag actcgaacac cttttgcgcc atagtgagct cagtatctgt tttctgaata
      781 ttttcgtgga tagaaatagc agatagatca ttttcttgga tcaacatggc cattttaaaa
      841 tctttaaatc cattataacc aagttttttt gtaaattgaa aaaaagtaga gtcagcaatg
      901 cccagttcac ttgctaaatt gctaatagga ctatgactta ctttgcttgg attttctaaa
      961 atataatcgg cgatagcctg ttcttttggg ctgaagtcgt tgtacattgt tttgatttgt
     1021 agtcttatgg agacgctcat gagaattcct cattttcata ataagattat cattttttcc
     1081 gttctcatta atgttaaaga tacaaggaga gtaggaaaaa atcaaacaaa aaatattttt
     1141 gtaaaatcta tttacaaaaa aatttttttg tataatgagg gtataagaaa tggaggcgct
     1201 atcatggaaa ttggtgtaat tggtttggga aaaatgggat tgaatttagc tttaaactta
     1261 aaagagcacc atcacaaagt acaaggactc gacatttcaa gtacagctgt tagtcaggca
     1321 agagaaaatg acattcctgc ttatcaagag atctctgaat ttctagctac ttttgagcag
     1381 aaaaaaatca tctggttaat gcttcctgct ggagagataa ccgaaaacat gttaagaaaa
     1441 ctttttccaa aactcggacc aggagacatt attattgaag gcggaaactc ttattataaa
     1501 gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa tcggttactt ggattgtggg
     1561 acttctggag gaatcgaagg agcacgacat caagcctgct taatgattgg aggagataaa
     1621 gaaacttttc tatccgtgga acaattattc aaagatgtag caattgaaag aggatatctg
     1681 tatgctggtg catccggaag cggacacttc ttgaaaatga tccataatgg aatcgaatat
     1741 ggaatgatgc aagcaatcgg tgaaggattc caacttgtac aaaaaagtga gtatgctttt
     1801 gacttggaac aagtcgcacg tgtttggaat catggttcgg tgattcgcgg ctggctgatg
     1861 gaaatcatgg aagatcagtt aaacgaacat cccaatttaa gtaattatcg gggaatcgtt
     1921 tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc tagaaatggc tgttccagtt
     1981 ccagtaattg caactagttt gttcatgagg aatctttcac aggaagaaga tagtttttca
     2041 gcaaaagtag tttcagcact aagaaatggg tttggcggac atgaaatagt tgaaaagggt
     2101 gagtaaaatg ccagattatc atatagcaat cgttaattcc agcagtttcg gaagagtctt
     2161 tccacaccac ttggaaagat taaagtcagt tggagacgtt catttcttta atgttgatca
     2221 agagattcca ggaaaagaac tagcggagtt acttaaagga ttcaatatca ttattgcaag
     2281 tgttacgcca ttttttcgac gagatttttt tgaaaacaaa gatgaactgc tattgatttc
     2341 ccgacatggt attggttaca acaatattga tttaaaagca gcaaaagaac accagacaat
     2401 cgtatctatc atacctgctt tagttgaacg aaatgctgta gcagaaaata atataactaa
     2461 tttgttgtcc atgcttcgac gcacgacaca agcacaagca agagtcaagg acgatcgatg
     2521 ggaagatcgg gcggagttcg tcggtaggac gctgtttaac aaaacagttg gtgtgattgg
     2581 tgcaggaaat accggaagcg gagtaatcga aattttgcgt aacggttttc gctgtgatgt
     2641 tctagcttat gatccttaca aatcagattt atacatcgaa agctttggtg caaaaaaagt
     2701 agaacttcaa gaattactgg aacaatcaga tatcatttgt ttatgtgcga atttgacaga
     2761 agagaactat cacatgattt ctgataaaga agtcgatcag atgaaagaaa atgtttatct
     2821 atctaatagt gcaagaggcg ctctagttga tgaagaagcg atgattaatg gtctgaaaag
     2881 cggaaagatc gcaggatatg ccacagatgt tttagaagaa gaaccgggac gtcaagatca
     2941 tccgtattta gcgtttgaac aagtagtgat tacgccacat atatcggcct acacaaggga
     3001 atgtttagaa gcaatgtgtg aaaaatgtgt cagagatgtc gaacagatca gtcagcacct
     3061 tcttcctgaa cgttctgtcc agacaaagag caactatata aaaggatgat gaagaaaatg
     3121 gtgaacggtt taaaagtgaa aacaggccct caattttatt tatatgaaga aggtggtatt
     3181 tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa gagtactggt cacccacgga
     3241 acggtatcat gggaaaaggc gctacctaaa ttagtatttt tgaatgacga aacgattcaa
     3301 tttttttatc atcgttattc tggagaatgt agttatgctg aagccagacg aatagctaca
     3361 atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg cggaggaaaa ctgaccgatc
     3421 tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg agtcatccct actttagcta
     3481 gtaattgtgc tccatggaca cctcttgctg tcatgtataa ggagaatgga caagcagaag
     3541 ggaaaacaga acacttttac agacaagctg ctttttttat aacagatccc caattagtga
     3601 ttgattcccc tgttccatat ttcattgccg ggttagcaga tacactagca aaatggtatg
     3661 aatctgagct catcttagaa caagattttt tgcaagatga atcatttttg aagctagcac
     3721 aagatactgc gaagatctgc aaagaagaga ttttgaatca ttctgctaaa gcaatccaag
     3781 atatggcaca aagaaaactg tcatcggagt tcaaacagct ttcagagatt atctttgctg
     3841 ttgctggttt agttggagga ttaggagata aatacgcaag aaatgtggca gcacatgcta
     3901 tgcatgacgc tatcagtaaa tatattccgg aaagccatcg ttttctacat ggagaaaaag
     3961 tagcgtatgg gattttctat caactggcac tagaaggtcg ctgggaaaaa atagacgagt
     4021 tatatccttt gtatcgggaa ttagaactgc cggtttctct agaacaaatg ggattatttc
     4081 cgaaagataa acagataata gataaaatgg ctgaatttat tgattcgaaa gagaaagtgc
     4141 atttgattcc tatagaggta acacagaaat cactgataca gtcaatgatg gaactggaag
     4201 gttatatcag aaagatctga aggaggagga cacatggaaa cattaagtgt agtacaaagt
     4261 ttgttgattg ctttatgggt agccgcggtt atgtcccgct ggttaggtgg aggagccacg
     4321 ttgaccttac gtttttctcc tttgatgact ggattagtcg tcgggttgat catgggggaa
     4381 gttgcacaag cgatgattgt tacagccgct ttgcaaatga tttatatggg ggttttttct
     4441 ccaggaggat caatgccagc tgaaccatct attgcagcag ctatcgcagt accagtcgca
     4501 ttattagggg atctgtcgcc agaagcagcg attgccgttg cggtgccagt cggattgctc
     4561 ggaagttact tgtatcagtt tcgttttttc atcaatactt ttttaggtaa atatacagac
     4621 aaagctgtag aagatttgaa tacaaaagct atggcccgat caattattct ttatccaacg
     4681 gttgcctcat ttttattatt cgttccgctt gttttcattg ctttatattt aggggcgcca
     4741 ttgatcgctg atgtaatcaa agctttagag ggaacagtcg ttatccacat tcttgaagta
     4801 gtaggtggag ggttagcggc aatcggtatc gcgacaactg tatacgttat cggtcgaaaa
     4861 gattatctgg tctttttctt ccttgcttat tttatgagtg ttgttttgaa atctttagat
     4921 attacgatgg ttacttatgc catttttggg attattatcg ctttgatctt tgtccagtcg
     4981 caaaaaggga aacaagtaca gcctgccaca agcaataaca attcagcatc ttttgatgac
     5041 gaagatgact atgatgacgg attttaattt aggaggaatg ggaaatggct gaaataatgg
     5101 ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga tgtaacaaaa gcctatctga
     5161 gatggcattt tgcaaacgaa attcctcact cttttgaacg ttacttggcg ccatcactac
     5221 tatacggaat gatgccaatc ttgaaaaaac tttataaaga tgaagactct ttaaaagcag
     5281 catataaacg gcagttactg ttcttcaaca ctcaattaag ctggggtggc ggtgtgatta
     5341 ctggcctaat gtcttctatg gaacagcaaa gagcagaaga agagtatgaa gaaaaagaga
     5401 tcttgatgca ggatgacttg atgtataaca cgaaagcggg attgatgggg gcactagcag
     5461 gaatcggaga tgcaatcgat tcaggaaccg tccaatatat ttttatcgca atcgcggtac
     5521 cttgggcaca acagggaagt cctttaggcg cactttttcc ttttatctgc tttgggttgt
     5581 atcaggtttt actaggggta tttttcgcaa gacaagcttt cactatgggc cgtaatgcta
     5641 caggcctaaa gcaaaacact ggtgttcaga cagcgattga aatgctatca gttttagggt
     5701 tgtttatgat gggggtgctt gcgggaaact atgtaaaagt agaatcttca ttgaaatttg
     5761 cgatatccgg acgtgagttt gttatccagg aaattcttga tcaaatcgtg ccagggatat
     5821 tacctttagc tgtcgtaatg ggtgtgtact tgttctatac gaaaaaagga ttgaaagtca
     5881 cacaagcatt attatggctg actggaatat taattgtgct agcaggaatc ggtattttat
     5941 aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt agcgcgtgtg gatgagcgtt
     6001 tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag tggagtgaat tccatttttg
     6061 ttgtagacga agttgtagca aaagataaat ttatgagaga tctttacaaa aatgcaggaa
     6121 gccgtacagg acaaaaaaca attgtcatta caccagagaa agctaaattc tattgggatg
     6181 aatatcaatt taaagaatac aattgtattt tgatcgcaaa aacagtccaa acgatttacg
     6241 acttagtaaa acacggaatc cccatggaag aattgaatat tggagggatt gctcaaaaaa
     6301 atcctgatac agatctttta gtgacgaaat ctgtttattt aaataaatca gatgctgaaa
     6361 aattgaaaga aatgaatgaa caatatggcg taaagaatat ttattttcaa gccactccat
     6421 ctgcacctaa aacaggatta aaagatgtat tgaacaagtt tgatttgtca taaacaaatg
     6481 cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa cgagctgttt cgtgattggg
     6541 aatttcgtta tcgctatatt taccgtaagc gccgtacaaa aaagagtaaa cagcgttttc
     6601 tttcagcatt agtatcagat atttacagta tgagaacaga tgtgacagtc attgcttacg
     6661 atacgctagc ctaccgatca aaaaatattt atgttggaga tatcgaaaaa gcagagaaag
     6721 ttatctgtac ttattacgat acacccgttc atgcattagg aagttatttc atgtttgatt
     6781 ggaaagacca aaggaaaaaa acaatctact ctatattatt gtccttcatt ttgttgttca
     6841 gtttaggttg gtggggaatg atgatatata acaaaaatcc tcatcacgta ttcgatctgc
     6901 tatccgtata aacaggtatc actgtactag catttgggag ctactttttc ttattgggaa
     6961 aagcagctcg cggatggcct agcagacaaa cttttattcg aaatacttca tccatattaa
     7021 cgatgctaga aatgatccgt acgattgatg atccgagtgt agcgtatgcg tttgttgatg
     7081 aaggctgtta tggagaaaag ggcttagaca gcgtgagatc gggtatgaaa aaagaggcta
     7141 ttctttttta tctagactct gttggggcag atacaccgct acagttttcg ggaaattatt
     7201 tttcaaataa agaacagtgg ctgaaacaag ttgataagtt gaaagaaaaa aacgtcaatt
     7261 atatttttag tgcaagaaaa aagcaagcgc agttcttcta tttgaccaaa acagatttac
     7321 gagggaaaac atttaattgg caaaatgcca atcagattat tgctcttttt cgataagtaa
     7381 aaagtgcaaa acaaggagtg agaagactaa tgttggaaat catgatcgtt acacatggta
     7441 agttgagcga aggactgaaa gatgcggcgg aagtaattgt cgggacatcg agccatattg
     7501 aaacaatgtg cttagagtct ggggaggaca tccaaacatt cggagagaag gtcaaagaaa
     7561 aattaactat gatcaacaca gcagaaccca tgctagtatt tgttgatcta attagcgcaa
     7621 gtccgtacaa tcaagcgctc ttggcagtca atgaactgac tgaagaaaag aaagcagcca
     7681 cttatgtcat agggggcgtg aatctgccaa tgttgttaga agcaatcaat catcagatgc
     7741 ttcacacacc gatagaagaa gcggtggaac aaattgtcaa acaaggaaaa gataatttaa
     7801 ctgtttggca tgcttcagcg gaagaaaaaa aattagatga ggacgatttt tagaaggaag
     7861 gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa aaggggattc gatcccatta
     7921 aaatatgttc gccaaatacc gggaataaat gggataattg gaactttgct gactaaaatg
     7981 cctggagatg tttgggagat ttcagaaatt gaggagttga aagcatctgt tgagagccaa
     8041 ggactggaac tgcttggaat tgaaagtgta gcgatccacg atgcaatcaa ggcagggaca
     8101 gatgaacgag attactatat tgataactat atacgaacaa tcaaaaactt gtctcgatgt
     8161 ggggttcgat tgatttgtta tagcttcaaa ccaatttttg gatgggcgaa aactgatttg
     8221 tttaatgaaa acaacgatgg aagctattct cttttatacg atcagaaaat cgtcgatcag
     8281 atggagccag gagaaatgta tcaattgata cacagccagt caaagggatt caaattgccg
     8341 ggttgggaag aagaacggtt gaaaaaattc gctcagctgg aaaaactgta tgaaggaatg
     8401 accgaagaag aattatttga aaatctgact tattttttag aaaaagtgat tccagtgtgc
     8461 gaaaaagcag atgtaaaaat ggctatccat ccagatgatc cgccatggga actttttggt
     8521 tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa ttctaaatgc agtagattct
     8581 ccatataatg gactaacgct gtgcacagga tctttagggg caaatccgga aaacgattta
     8641 gttgaaatca tccatgaggt aggttcgcgc atccattttg tccacttcag aaacgtggag
     8701 tttttaggag aaagaaagtt caaagaaacg gcacatccct ctacagaagg ctcccttgat
     8761 atgtatcata ttatgaaagc gttggtgcaa gaagggttcg acggtcctat acgccctgat
     8821 catggtagaa ccgtgtgggg tgaagtggct atgcctggat atggcctgta tgatcgtgca
     8881 atgggactca cttacatgca aggtctatat gaggcgataa gtaaggaaaa accactggga
     8941 gagcattatg aataaaatca agctcttcat ctttgatatg gatggccttt tattagaaac
     9001 tggacgttta gcttatcgtg cttatgtaaa atcagcacaa aagtatgatt atgaaatgcg
     9061 taaggaagtc tattatttac taactggcca aacagaaatg gcgataagag aacaaatggg
     9121 cattttgtac ggagaagatg ttccatatat caaatggcga gaagcaatca atcaatataa
     9181 agaaaaaatc gtgaaagaag ataagagagt atatacgaaa aaaggagcag aagaaatttt
     9241 atcttttgca aaagaaagag gaatccatac aatcgtggct tcgtctaata cacgggtaaa
     9301 agtagaaatg tatttaaaaa tggaaaatct atatgccttg tttgatcaaa tcatttcagg
     9361 ggatgatgta aaaaaaggaa agccggaacc agagatcttt ttgaaagctt gttcaaaaat
     9421 gaatattcct ccaagtgaag cacttgtatt tgaagactcg attgcaggga tcgaagcagc
     9481 aaggcgagcg ggcattttgt cttttctcat caaagatcat ttgatagggt tgcccgatca
     9541 taatgggaag cataagttga aagtagatgt aagtttatat gaatccaata aagcagacta
     9601 tgaattttat gacctgcatc aagcaaaacg atttttagaa agtaaagggt tgtatctcta
     9661 ataaagagga ctattttcta gtcctcttta tttattgtcc tgcacgactg agggaacaga
     9721 tgaaaaagaa gtaatttttt attttgaagt atctacctta tttgtagtga acaatttaga
     9781 acaaacacaa aacgcatcta ctgttat
//
LOCUS       DAYYAR010000103         9741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000103 DAYYAR010000000
VERSION     DAYYAR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.104"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..413
                     /locus_tag="ACHAT0_002154"
     CDS             15..413
                     /locus_tag="ACHAT0_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8452081.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            650..1192
                     /locus_tag="ACHAT0_002155"
     CDS             650..1192
                     /locus_tag="ACHAT0_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8452082.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1275..1826)
                     /gene="lepB"
                     /locus_tag="ACHAT0_002156"
     CDS             complement(1275..1826)
                     /gene="lepB"
                     /locus_tag="ACHAT0_002156"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8452083.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(1978..2205)
                     /locus_tag="ACHAT0_002157"
     CDS             complement(1978..2205)
                     /locus_tag="ACHAT0_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8452084.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2202..2720)
                     /gene="msrA"
                     /locus_tag="ACHAT0_002158"
     CDS             complement(2202..2720)
                     /gene="msrA"
                     /locus_tag="ACHAT0_002158"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8452085.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2740..3453)
                     /locus_tag="ACHAT0_002159"
     CDS             complement(2740..3453)
                     /locus_tag="ACHAT0_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8452086.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3456..4352)
                     /locus_tag="ACHAT0_002160"
     CDS             complement(3456..4352)
                     /locus_tag="ACHAT0_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8452087.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4510..5352)
                     /locus_tag="ACHAT0_002161"
     CDS             complement(4510..5352)
                     /locus_tag="ACHAT0_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8452088.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5484..6137
                     /locus_tag="ACHAT0_002162"
     CDS             5484..6137
                     /locus_tag="ACHAT0_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8452089.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6200..6727)
                     /locus_tag="ACHAT0_002163"
     CDS             complement(6200..6727)
                     /locus_tag="ACHAT0_002163"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8452090.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6747..7694)
                     /locus_tag="ACHAT0_002164"
     CDS             complement(6747..7694)
                     /locus_tag="ACHAT0_002164"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8452091.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7706..9589)
                     /locus_tag="ACHAT0_002165"
     CDS             complement(7706..9589)
                     /locus_tag="ACHAT0_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8452092.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
BASE COUNT         2627 a         1912 c         1701 g         3501 t
ORIGIN      
        1 aggagacgaa acaaatgaat agtatggttt ttgttaattt tcctgttgct gatgtcaatc
       61 gttccgttgc tttttatgaa aagctgggtt ttaaaaaaaa tgaagaattt tctactgaag
      121 aagctggttc aatggtatgg gatgacactt tttggataat gcttctctct catgattttt
      181 ataaaaaatt tataaaagat aaagaaatag ctgataataa aaaaacaagt ggtgctttga
      241 tttcgtttag tgttgaaaat gcggatgaag taaaaaaatt tgctcaaatt gcaaaagaaa
      301 atggtggtac tttccacgaa gtagatatgg gaatgcctga agaagaaatg ttttcattag
      361 aagtccaaga tctagacgga aatacattag aacccgtatg gatgaagatg taagcagata
      421 actaatctga cgttttttat aacaaaaaaa caaaggcgga tgatacgata agtattctcc
      481 gcctctattt tttttattcg tacaatctct tttttacagt ttataaaagc agtcttctca
      541 cccttttttt aattgttaga tgccagtgta tgtaaatcac caatgtgtca tgacacattg
      601 gtgattttat ggtatccttt tattattaac tgacaaagga gagaatattt tgaacacttt
      661 gattgcaaaa gacgttttta gattgcccga aatgaatgca agattgttgt cgacctctgc
      721 tcttttatta gcccttgatg tcatcttata caagatagcg attggtcctg cttattttca
      781 aatcacattc ggatttttaa gtatggcgct tatgggttat ctatatggac cgatctgggc
      841 tggtttgctt gaaatgttgt cgaacacttt aaatttcacg atatttggtt caggaacgtt
      901 tcaagctgct tttcttatca cggctttcct tggtggattt attaatggct tatttttata
      961 tcataaaaag ataaacctgc cttccgtcat cgctgcccaa ttattgatta tgatcccagt
     1021 cagccttttt atcaattctt ggctgatgtc tgttttgttt ggtgcagatt tcaaagtaat
     1081 ttttctaggt agattcatca ggaatatgat ccaaatccct attcaaataa tggttgttta
     1141 ttcatttatc aaaactttag aggctagaaa agtgtttaaa cgagctgttt gattttacgt
     1201 actaaggtag taaactgatt ttggattaaa caataaagcg tgcagtctca acagactgca
     1261 cgctttattg tttctcactg gatccatttt atttcatcaa gtggataata gacaaatctt
     1321 gctttaccta gaatatcagt tttattgatc gtgccaaacg aacgactatc ttttgaaact
     1381 cttcgattat ctccaagtac aaaataatta tcttccggaa gcttggattg tcccgttagc
     1441 tcttgtagtg taaagtccgt cgtataggat gcatttgcat gatctgattt caggttttta
     1501 gttagataag gttccttaac tactttccca ttgatcttca attgatcatt ttggtagctg
     1561 acattttctc ctggaagacc gattactcgt ttgatataaa tcgtgccatc tgctaattga
     1621 aaaacaacaa tatcaaatcg tctgatctcc gaaaattttt ccatcacaac catatcacct
     1681 tgcttcaaca cgttttccat tgaattgcct tccactggta ccgggatgaa taaaaatccc
     1741 cgcaacatga acgcaaggaa tacagaaaca agtaagtatt tgaatacaag ccagaagcga
     1801 ttaatatagc gttgtttttt tgtcaatctc tatcaatcca tccctctttt tttcattctt
     1861 tctttattat aagaaaaata tcaaaaaaat gcgactagat agctgaaata aaaataattc
     1921 tttcaattag gctattggac aaaaaacttt tttgatcata aagtacaaaa ctatctctta
     1981 tatgtcatga ttattttcta aatatttttc ccatgcttcg tcaaagatat ccatggacgg
     2041 caaatagtcg acattcagtt ccagataatt agaaatttct tcgtagctag agctgtgctt
     2101 aggaaattgg atgtctttac cggcttctgt agcgaaaaca gataaagcat catgtgtgcc
     2161 accgcctcgc agtgtcatta aataatgaaa aaagcttcga ttcacccttc gttcctccaa
     2221 ttttcttcga taaaggcagc ccggcgtgca tggtttcttt taaaattttc aggatgcttt
     2281 ttataatact cttgatggtc ttcttctgct agataaaatg gttgggctgg ttcgattttt
     2341 gtaacgattg gctgttcgaa acgtccactt tcagctaact gtttcttact tttttcagca
     2401 atgatccgct gttcttctgt caaataaaag atcaccgggc gataattgtc accgcgatcc
     2461 tcgaattgtc cgaaagcgtc agttggatca gtttgctgcc agtaaagttc tactaattgt
     2521 tcatatgaaa tcaatgttgg atcatagata atctccactg cttctgtatg tccagtcgtt
     2581 tggcttttta cttgttcata agtcggatct ggtacatgcc ctccagtata tcctgacatc
     2641 actgtatgga taccaggaag cgtgtcgaaa ggctggatca tacaccagaa gcagcctcct
     2701 gcaaatattg ctcgttccat tatgattccc cctctaatac tactcatccg atgatttttt
     2761 attttctttt gtcttgcttt cagtactgct ggtgctagtt ttgttggtat ttgagttgtt
     2821 tgaagcatca gaggtatctt ttggcaaata tagactaaac cgaatgtcat catttactaa
     2881 gtcgatttta tctgctttaa tatacatccc gttttgcatt cggaattgat ccaacctaag
     2941 catgatagtc agatcatctg tattgacttc gatccaatct ggtaatttat agtttctctt
     3001 gatcatattc atcacatctt tgattggcaa actcaatgtt ccgatagaaa gacttctagc
     3061 ttttagttgg acatttccgt tttccatcac atacggatca aaatataaat agaaatcgat
     3121 aggaaaacct agtacttcga aagtaccatt gagtagtgct tcattttcca aataaaattt
     3181 atattcaata tcactatctt tttgatactc actaaggaaa aagctgatga tttgattgac
     3241 tttttcttta tttgaattga tcgttaagac aggatctcct tgtctttccg taagagctgg
     3301 tatttctggg agttctggtt cccgattagc aaaaatacgg ctgcctaaaa atacggtcga
     3361 accaagtact accccaagaa ggataaggaa ggcgaatttc cagtaatttg ttttctttgt
     3421 tttgtttgaa gatacgtcag atcttgtttt catgtttatt gatcatcctc cttctctaac
     3481 cattggtttt gtgtctcgat cagctgatct ctcactgcat ttgccatcaa ttgataacct
     3541 aaattatttg gatggaaatg atcttcttca tagagcacat tatttttgat gccatttaga
     3601 ttttctgtcg tgctagttgt ttgatctgtt ccttcttctg tgacacctac ttcctgattc
     3661 aatccttgat aaagcaaatc attgattggg ataaagtagc agttcggcat tttttttgtc
     3721 accgtttctg ttcctttgtt ccaattgtta acaattgtct gcatgtcagt gatctcagga
     3781 aagttcaaat aaaatggatt gtagatgcct aaaatgtaaa taggtgcggt tttattgagg
     3841 gatcgaaatt cttccaatag ttctgtgaca ttttcctgat agtttttcag tggcttttta
     3901 aaggaattga ccgttaaccc gaataaatta ttttggataa ctttcatcaa atcattaccg
     3961 ccaacagtca gtgtgataaa atcagctgtt ttgatattgt ttcgaatttc ttcttttgtt
     4021 tttactcgct ttaagatctg gtcacttcgt tcaccattta caccatagtt ttctgtttca
     4081 accgcggtca aattataacg gtcttttaga tcatttgcga caaggggaac gaatcctcct
     4141 tgcttggttt gatctcctac tccttcagtc agcgaatctc cgatagctat gtagtgaagt
     4201 ttttcttttt tatttggatc ttctctcaca gtagttactg gtttcattag tgggccagct
     4261 ttcggtatag aagcagctaa acctgcatag atgatgatcg caattaataa tgggaacaaa
     4321 acgaggatca aatgtcgttt cgttaatttc attttttcac ctcttgtatt cgattggttc
     4381 taatgttatc aaaaatttta tcaaaaaggt agtctttatg aaaaaagaaa acaattattt
     4441 tcctaatttt ataaagaaaa agagaataag ctgcaagcct ttcaggctga gggcttatcc
     4501 tcttttcgac taatctacat aatacatgat tgcaaacgcg tctttaccag tatgcgtagc
     4561 gataaccgga ttcgtatgca gtacagggat atgcatgtcc gggaataatt cttgcaaacc
     4621 ctctttgaat tcttgcgcca attcttttgc atccgcatgt gaaataccga tttgtcgcac
     4681 atttggcaat tctttcagtt catttttcaa attattaaac catttatcga acgttttctt
     4741 tcctcgcccc ttagttacag ggattaattc agaatgggcg aaattcatca ccacgcgtat
     4801 gttcaaaaca ctagacagca aaccagttgt tcggctgatt cttccacctt ttaccaagtt
     4861 atctagtgtg gatattccga tgtataattt tgtattgtct tgtacttttt tgacttcttc
     4921 aaggattttc tctactgttt taccttcttt tgctaattca gctgctcgaa ttacctgaaa
     4981 agataatcct tggtcagtgg tatcactgtc gataaccgtt actttcgttt ttgttagatt
     5041 actggcttgt cttgctgctt ctactgttcc actcaagcct tttgtcatat ggatcgaaat
     5101 cacttcactg ccgtcttctc ctagctcatc atacatcgag atgaattctc caattggtgg
     5161 ttggcttgtt ttgggcagat tttttgcttt cgccatcatt tccataaatc tttcgccgtc
     5221 tagttggtca tcatcgggat atactgttcc gtcgatcatg atggatagcg gcatgacacg
     5281 aatgcctaaa gcatcgcgta catttttttc catcgtacat gaagaatctg ttacgatttt
     5341 tacatttgtc attattttct ctctctcttt ctttaacaag atgccttatt catgttaaac
     5401 tacatgtaag acaatttcta tttttagtat aacagacaaa taatgatttt tcattaatta
     5461 cacctagtaa aggagccgtt ttcatggaaa aaacgaaatt taatcgaacg tatgaaatat
     5521 taaatgaagt gtttaatgca gtcacccatg gaattgggac tggattgagt atcgcgggac
     5581 tagtggttct tttagtaaaa ggtgcccact tgcattcctc cctccatatt gtctcttatg
     5641 ctatctatgg atcgatgatg attcttttat ttttaacgtc tactttgttt cattcattga
     5701 tttttacaaa agcaaaaaag gtgttccaag tattcgatca tagttcgatt tttcttttga
     5761 ttgcaggaag ttatactcct ttttgtttgc tgagtatccg aggatggcta ggttggactt
     5821 tatttgtatt aatctggttg tttgcgattc ttggcattat atataaagcg ttgacgttgc
     5881 ataaacggga gagagtatca aaagtatcta cgatcatcta tatttttatg gggtggctct
     5941 gtgtcgtggc tgctgttcct ctttatcact ctcttggaat gactggtgtt attttgatgg
     6001 tagcaggcgg gctttcttac acgataggcg cattctttta tagcttgaat tctatccgct
     6061 atatgcatgt tgtttggcat ttgtttgtta tgttaggtgc aggttttatg tacttctctg
     6121 ttttattctt tacttaataa aaaaaataaa gctatggatg ggacagctct aaataacagc
     6181 tagtctatcc atagcttttt tatatcgtca attcgcctgt cccgcttttt acaggattct
     6241 cattacgctg atacgtttca aattgatgcg gataacgatt tttttcatcc atttctcctt
     6301 gactagtatt gatcagtgac caatcagacc agtcaacagg tggaaaataa gcatctccat
     6361 cgaatacatg atggatgact gtacggtata atatcgtaca aaatggcaga aattcttgat
     6421 aaacagctga ccctccagcg atgaaaaaga taccgtctgt ttctttttcc agagcaagca
     6481 cgtcttccac actatgcatg acatgcacgc cttctgcttt ataggaagaa tctctcgtca
     6541 aaacaatcgt ctgacgattc ggcaacggac ggctacccat cccttcgaat gtttttcgtc
     6601 ccatcactag tgtatttgct tctgtcattt gtttgaagaa tttcagatca tttggcaaac
     6661 gccaaggaag acggtcttct tttccaatca atccgttttc atcctgtgcc caaattgcag
     6721 ctaacatttt ctcatccttt ctgattctaa acagcgatag gagctttgat cgccggatgt
     6781 gggtcatatc cttctaattg gatatcttcc atctcaaaat caaatactga ctgcttttcc
     6841 tgattcaaaa ctaaagtcgg aaatgcgcgt atttctcggc tcaattgttc tttcatttgt
     6901 tcgatatgat tgatataaag atgagcatct cctaatgtgt gaacaaattc gccaaccgct
     6961 aaacctgttt catgtgcaat taggtgagtc agcaacgcat aacttgcaat gttgaacgga
     7021 acgcctagaa acacatcccc actgcgttga tacaattggc agcttagccg tccctcttga
     7081 acataaaatt gaaacatcgt atgacaagga ggcaaagcca tgctaggaac atcttccgga
     7141 ttccatgcag aaacgatcaa tcttctagaa tcaggagtct ttttgattgc ttcgatcact
     7201 tgtttgattt gatcgataaa gccaccgtct tttgtttccc agtgacgcca ttgatagccg
     7261 tagatgtgtc caagatcgcc gtatgtttca gcaaaggaat catcggttag gatcttctcg
     7321 caaaaaagct tgctctgctt tttatattct tcattgaatt caggatctgt caaacatcta
     7381 tggccaaaat cagtcatatc tggtccatta tagtctggac ttttgatgta gcgttcaaat
     7441 gcccattcat cccaaatatg gtttttattt tgtaatagat agcggatatt cgtatctcct
     7501 tttaaaaacc aaagcaattc gcttttgatc aatccaaaag gtactcgttt cgttgttaac
     7561 aaaggaaacc ctttttgcag atcaaaccgc atttggtatc cgaataagct taatgtgcct
     7621 gtgcctgttc tgtcttcttt acgatgccct tcttccaaca attttcttcc taagtcaaga
     7681 tacgcttgtt ccatcctttt tcctcctaat cttctaccca ttcactcaag tattcccacc
     7741 gtgccatttt ttcttcaagt tgtgcttctg tttcagatac gtcgttttgc aattcttgta
     7801 atcgagtgaa attatctcct tgatgattca tctcttcttg gagcaatgag attttttctt
     7861 caagttctgc aatctcatct tcaatcgttt cccattcttt ttgttccatg taagataatt
     7921 tcttcttttc agcaggttcc ttgctctgta tttttggttt cgctttttca gcaggctgct
     7981 gggtctcttt ttgcttcagt aaataactgg acatgtttcc gtaataaagt tcttgttttc
     8041 cgtttccttg gaatactaat aacttatctg ctaccttgtc caagaaataa cggtcatggg
     8101 aaacagtgat cacagcacct ggaaaacttc taaaataatc ttccaaaatc gtcaatgttg
     8161 ctatgtccaa atcattggtc ggttcatcca ataataaaac attaggttgc tgaatcagca
     8221 gtttcaacaa aaataaccgg cgtttttctc cgcctgaaag tttgcctatc agtgtcccat
     8281 gcataaatct tggaaataaa aatcgttcaa gtaattctgc tactccgatt tgactgccat
     8341 ctgctgtttt tgcttcttca gcagcttctt gtaagtaagc aatcatccgt ttatctggtg
     8401 ccatttcttc attttcttgt gtataatacg caagatgaac cgtttcaccg atagacattg
     8461 taccgctatc caggggaatt ctgccagcta gaatattaag caatgtcgat tttcccgcac
     8521 catttttacc agtaatacct agtctttccc gagattgaat caataaatct aaatgttcta
     8581 gcagtgtttg gtcatttata cggtaactgc catctttgat ttccagtact tttttcccta
     8641 aacgttgtgt cgctaggttc agctctaaat catcttcttg gtttacttgg tacaagttct
     8701 cctttagatc ttcgaaacgg ttgatccgtg cttgctgctt ggtgccacgt gcctgtacgc
     8761 cagccctcat ccaagctaat tcctgtttat aaagccgctt gcgcttttct tcttgttcta
     8821 cttctacacg atcacgttct gctttttcta agacgtaagc ttcataattt cctttatact
     8881 cgtataattt tccaaatgaa agttcgaaga ttcggtttgc tactctatct agaaaataac
     8941 gatcgtgggt aaccatcaat agtgcgccac ggtattgttt caaatagttt tccaaccact
     9001 cgatcgcttc ataatctaaa tggtttgttg gttcgtctag taatagtaaa tcgggttcat
     9061 caatcaacac ttgcgccaga ctaactcgtt ttttctgacc acctgataaa gtactgattt
     9121 ttttatctaa ttcactgatt cctaattttt gcaaaataat ctttgcgttg gtatctgtag
     9181 tccaagcatc ttctttattc atttgttctt ctgctttagc ataacgtttt tgcacatcag
     9241 ggtcttctcc gttttcccct agttcgatca atgcttcctc atatgctcga acagtttgga
     9301 tcaaggggct gtttccttga aagactgctt ggagaacagt taattcatcg gaaaattcag
     9361 ctgcttgaga aagataacca atacggtaat cagttggata aaacactgtt tgccgatcac
     9421 cgtcgctgct atcgataccg gcaataatat ttaagagact tgttttaccc gtcccgttcg
     9481 tcccaatcaa cccgatacgg tctttttcgt ggaccaaaaa agaaatctgg tcaaacaaat
     9541 ctttttcacc ataagttttt ttgaggtcag ttacttttaa ttctttcaac atgttccctc
     9601 cttatcttgg atattgtatc ataaaaatag tgaacttcag gaaaattttt ttggtatttg
     9661 ttctttttgt gttctcaaaa aaagaacagg accgattcga tcctgttctg atatgttttg
     9721 ttttttataa aacttgaaaa t
//
LOCUS       DAYYAR010000104         9697 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000104 DAYYAR010000000
VERSION     DAYYAR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.105"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..125)
                     /locus_tag="ACHAT0_002166"
     CDS             complement(<1..125)
                     /locus_tag="ACHAT0_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFM8452093.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="ACHAT0_002167"
     CDS             complement(143..463)
                     /locus_tag="ACHAT0_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8452094.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            757..897
                     /locus_tag="ACHAT0_002168"
     CDS             757..897
                     /locus_tag="ACHAT0_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452095.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            1195..1452
                     /locus_tag="ACHAT0_002169"
     CDS             1195..1452
                     /locus_tag="ACHAT0_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFM8452096.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(1423..1731)
                     /locus_tag="ACHAT0_002170"
     CDS             complement(1423..1731)
                     /locus_tag="ACHAT0_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452097.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1809..2555
                     /locus_tag="ACHAT0_002171"
     CDS             1809..2555
                     /locus_tag="ACHAT0_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8452098.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            2570..2773
                     /locus_tag="ACHAT0_002172"
     CDS             2570..2773
                     /locus_tag="ACHAT0_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452099.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            2789..3127
                     /locus_tag="ACHAT0_002173"
     CDS             2789..3127
                     /locus_tag="ACHAT0_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8452100.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            3114..3293
                     /locus_tag="ACHAT0_002174"
     CDS             3114..3293
                     /locus_tag="ACHAT0_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452101.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3336..3806)
                     /locus_tag="ACHAT0_002175"
     CDS             complement(3336..3806)
                     /locus_tag="ACHAT0_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452102.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3893..4591
                     /locus_tag="ACHAT0_002176"
     CDS             3893..4591
                     /locus_tag="ACHAT0_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8452103.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4769..5110
                     /locus_tag="ACHAT0_002177"
     CDS             4769..5110
                     /locus_tag="ACHAT0_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452104.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            5103..5774
                     /locus_tag="ACHAT0_002178"
     CDS             5103..5774
                     /locus_tag="ACHAT0_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8452105.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            5780..6472
                     /locus_tag="ACHAT0_002179"
     CDS             5780..6472
                     /locus_tag="ACHAT0_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8452106.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            6469..7218
                     /locus_tag="ACHAT0_002180"
     CDS             6469..7218
                     /locus_tag="ACHAT0_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452107.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            7230..7499
                     /locus_tag="ACHAT0_002181"
     CDS             7230..7499
                     /locus_tag="ACHAT0_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452108.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            7505..7669
                     /locus_tag="ACHAT0_002182"
     CDS             7505..7669
                     /locus_tag="ACHAT0_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452109.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            7662..7961
                     /locus_tag="ACHAT0_002183"
     CDS             7662..7961
                     /locus_tag="ACHAT0_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452110.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            7961..8263
                     /locus_tag="ACHAT0_002184"
     CDS             7961..8263
                     /locus_tag="ACHAT0_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8452111.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            8303..8668
                     /locus_tag="ACHAT0_002185"
     CDS             8303..8668
                     /locus_tag="ACHAT0_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452112.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            9158..9298
                     /locus_tag="ACHAT0_002186"
     CDS             9158..9298
                     /locus_tag="ACHAT0_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452113.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            9295..9477
                     /locus_tag="ACHAT0_002187"
     CDS             9295..9477
                     /locus_tag="ACHAT0_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452114.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            9490..9684
                     /locus_tag="ACHAT0_002188"
     CDS             9490..9684
                     /locus_tag="ACHAT0_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452115.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT         3544 a         1547 c         1939 g         2667 t
ORIGIN      
        1 ccatatagat tttcattcaa aaaaatgatt ctaggcttcg gataatagaa gccaggctct
       61 ttcatttcca tgtaaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaac ccatcactcc aactattcat tttcctctaa agctttagcg attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtttt
      241 ctgataaatc cacatgttta ggggtttctt tttcttcgcg ccccagaaga tagtctacag
      301 agacatcgaa atagtcagca atttcttgca aacgttctgt agaggggttt gagttcttaa
      361 gtctatataa gacgttttta ccatagccta ggtcttcttc aactttattt aatgattttc
      421 cctgtttttt cgctaactct tttattctct caaacgttat catatcaata ttcctttcgt
      481 tcaagaaaaa atatttaact ttatttgtta aaattcgttg actattttaa caaaaggtgt
      541 taatatcatt cttgtaaaca agtttaacaa ctaaaaagac aacaaaaaac actattgatt
      601 aataaatgcc aaccgccaag aaagctttta aaatcaatgt ttatatgtct tatttaacta
      661 tgttctgatt ttaacactgt atgttaattt agtcaacgca gttttttaaa aagttgttaa
      721 atttgtttac gaatataaaa gaaaggagag aagaatatgg aaaaaacagt ctcggcaaaa
      781 atcgaagatt tgaaattaga catactgaaa caagcaaaag tggcgatgga acacgcggta
      841 gataaagaag actctgccat ggttgcagcc atagcagaga ttttagctca cgtttagtca
      901 tcgtttttac ggtcttctgg aagctgacca tagataacag cataatgaat atctaaataa
      961 gtttgaacaa tttcttttgg cgaaattgat tcgcctttag tagatacttg tgattcgtga
     1021 tttagccatg caacaactaa atccgcagcg atttcaaacg gcgttacact ttgtgtcagt
     1081 tgccataaag aatttcacaa tatgtatggt cgtggtaata ataccaaaga gcaatttgag
     1141 gaatttatgg aaattaaaaa atacaaaaaa tcatttatta aaggaggcta aacaatgaac
     1201 aaggaaatga gccaaacaat caaggtacaa aaaatgatag atgatctaac acatggaatt
     1261 gatagccaag ccgataaaat catcaaggaa ttgcaaggac aaaaagttaa ggatgcaaaa
     1321 atgcttctca agactatcaa ctttgaaatg aatccaacaa aaagaaagct cgccgatgtg
     1381 ttggaagaaa agttggcttc cgctatcaac gagcaagaat tactatttga aaccgatact
     1441 tttaacagct gatgttttat agatgacagt ttcatccgaa tctatatcat cgccgagaga
     1501 aaagaatgtt gcttttgaca aaaagccgat aagacctaca agcggtcttg atgttgcaag
     1561 agtagatacc ccaatatcac cttgcaaggt attcacgtaa atttgagaag aatagaacat
     1621 tccatctttt tcttcgatat ttgacgcatc atcgtatcca acgaccaaag tatcggcaaa
     1681 tattttgatt ttttctccat caagcatagt aattacaaat tctggtttca taaattttca
     1741 cctccttaac aattatttca gcctgtcaca ctgataagga aattatacca aagaaaggaa
     1801 tgagaaatat gaatacacca caaattttta atttcgagca aaacgaagtt cgaactattt
     1861 tagtaaatga tgaaccatat tttgtaggaa aagatgtagc agacgttcta ggatattcga
     1921 atccgcaaaa agctattcga gatcatgtgg acttggagga taagacgcag aacgattcgt
     1981 tcaccgtcaa tggaacagca gttgttttga ttaacgagtc aggcctttac agtttaatct
     2041 taaaatcaaa acttccctct gccaaaaaat ttaaacgttg ggtaacgagt gaagtccttc
     2101 caacaattag aaaaacaggt agctattcaa acgtacctca aagttttgca caagcattgc
     2161 gtttagcagc ggatttagaa gaaaagaacc aattactcga acaacaaatt gccgagtacg
     2221 aaccaaagat tagctactta gatacgattc tttcatcgac agatacggta gctacttctc
     2281 aaattgcagc tgattacgga atgtcggcaa ttgctctaaa caaattgcta aacgagttag
     2341 gtgttcagca taaagttagc ggacaatgga tactttaccg aaaacatatg aaccaaggat
     2401 acacaaaatc gcacacaagt gagataccga aagccgatgg cggcactaaa gttgtaatga
     2461 ataccaaatg gacacagaaa gggcgagtgt ttatttacaa cttattaatc gcagagggct
     2521 attaccctca aatggattta gaggaaattg gttagaaagg agttttagta tgactgacat
     2581 tgcagaaatc actcaacgag atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt
     2641 cttaacttac accatgcttg ctgaaagatt tggaattagc aaaagctact tatctttaat
     2701 tttaaacggt aaaaagactt ctgcagaagc aaacagaatt atagattcga ttatcactat
     2761 gtacgaattg taaagggagg aacagctaat gcaatatcta gaagcaaaaa tcccaattcc
     2821 agaaggctat gtaattatct cccaagtgga ttatgaggag ttaaaaaaag ctgatgatac
     2881 tggtagatgg atgacgttgc cagaagtgct agaacggatt aacagaaaat atgattggtt
     2941 tacttctaga gttttaaaga acccaagata tagaaatatt atcgatatcg agaaaaacaa
     3001 aaatggattt gtctattatc cagttgaagg aagagacaca tatctatttt taagaagtaa
     3061 aacacttgaa tttttagaaa caaatttttc ggaaatctta aggaggcaag cggatgggaa
     3121 aatttaatag agcgctagtg ttcagcgcac cgctaatcat ctacgcttta ggactttggg
     3181 gaagcaggca agcgttgata ggaacgattg tttacatggt ttggattttt atggggcttg
     3241 atgaagctga agctgagtac agagcgaaaa agccaaccga gggggctgac taaaaatgaa
     3301 tagaaaagag aaactagaat gtatattact attactcagt ttaattcttt cactaatttc
     3361 tctattggga agtttgtatt tttgatatca acaattttgt ggatttgggt tctatttaga
     3421 gtaaatatca tttttgtttg gtgattaagg agtttattaa aattaatttt ccctacttca
     3481 aatgcaaggt aacaagatat agcactttta ggaggaatat ttagcggtac agcttcagag
     3541 tatattctat tatcatcgac tgatcctcct ttagcaatta atttcttaaa tggagtagct
     3601 gaccatctgc ggtttaagtt agcggatgaa agttctaaat taactaacat agctggttct
     3661 gttgaatagt tagaaatgat tacttttgta taaatcatat ctgcagcgaa gtaagaagca
     3721 ttaagttcaa cttctagttg agggcgcatt ccttttattt ttatccctgt taaaaacgta
     3781 ctcaatataa aacttattat tgacatccat tgaaaaattg ttaagtgaaa aaaattcaaa
     3841 ataaacacca ccagttttta actaaattat accaaaaagg agagaagaaa taatgcaaga
     3901 attagtaatt ttgaaaaata aagaagctgt gactacgagc ttacaagtgg cagaaagctt
     3961 cgagaaaaaa catcaacatg ttttaagaga tattgatgca ctaaaaaaag atgtgtccaa
     4021 ttttggacag atgtttgtag aaggtaatga accagattca tatggcagaa atcgacgagt
     4081 tttcttcatt agtagagatg gatttttctt gctggctatg gggtttacag gaaagaaagc
     4141 tatctacttc aaacaaaaat acattgaagc attcaacgaa atggaagatg ttattcgcaa
     4201 gaatactgtt cctcaaacaa ttgaagatat gatgatctat caattagaag aaatgaaaga
     4261 tgttaaaaaa gatgtttcca tgcttaaaga tactatgcga attagcggac aacaagagtt
     4321 tgaaattaag caaaaaggaa atatgaaagt tatgggagtt ctagggggaa aagaaagccg
     4381 agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt tggtctgaat ttaaacgtac
     4441 cttttcaatc ccaagatatg gcgagttacc tcgtaagaga ttcgatgatg ctgtttcatt
     4501 tattgaaatg tggttgccag aaactgcgat ccgtatggaa attgatcaac tgaacagaca
     4561 acagagactt tttggtgatg acaatgaata gagctgaagc gctaagaata gggacggcaa
     4621 ttgctaattg ctggtggaaa tactataaac caatcatcct aagccaacaa catattgaca
     4681 agcaaaaagc atggcaacaa ataaaaaagt gactccgccg gcaagcaaag agtcacaaag
     4741 aaaacacatc ataaggagat tttagcatat ggaaaaagaa ctttccactc tagatcaata
     4801 tttgattgat cctgattggg gcaagccgaa aattgaggaa acaagtggtc gaaaaatcag
     4861 acgaaatctt ttgacgaatg aagaactagc ttgggatcaa gatgatttag gcaaccatgt
     4921 aactatttgg gatcatgttt atcttatcca tctatcgaag cattcgaata aacctgaata
     4981 tatttacgtc atcgaagatg gcttgattga tgcgctagaa gagtacgaca gagataactt
     5041 gattgatatc tcttattacg gaccaggtaa gaaatacatt gctgaaatgg aggcagaatt
     5101 tgatgagtga aatcaaaggg acaacgaact ttgaaaaact ttttagtcgt aagttaaata
     5161 aaattctcaa gaaaaaagga aattttgatt atttatcttg ggctcacgcg tgggagatta
     5221 tgaaaaagaa tgatccacag gcaacggtaa ctattaatga gtacaaacac tacagggttg
     5281 tttctggaac tcatcaagac tttcttgttg aggaatataa accttttctt atggatgaaa
     5341 ctgggactta tgtatctgtc tcagtaacgg ttaaaggaca cacggaaacc gaattatttc
     5401 ctgttttaga ttatcgaaac caaccagttg ttaaaccaaa tgcaatgcaa atcaataact
     5461 cattgaagcg atgctttgtg aaagcattgg ctctacacgg actgggatta tatgtatttc
     5521 aaggggaaga tattccaaca ccacctagaa tcgatacaaa gaaattaaac atgctagaga
     5581 cgattctaga agctttcaat gagcagatgg gtaaagatat gaccaaaacc ttaatcgaat
     5641 atgttaatga gcagacagat aaattagggc tcttagctga taacgttgaa actattgaac
     5701 agttaagcta tgagcaatgt gccttgatgg agcgagcaat agcagctaag agaaaagaat
     5761 tagataagaa gtgatatgag tgtttaaacc attaatcgat tcatattcag cggttctgaa
     5821 aaagttcaaa ggaaaagaca taagcgcaac catcaatgag gaagtgaaca ttgatcgact
     5881 aaagacgatg tatgacggct acgatggtga tcgagtcatt gaaattcgtt ttattgatcc
     5941 tagacgtttc accgtacagc aacgaaactt catctatgcg ctgataggcg atatttttat
     6001 cgatacaggc atgccaacgg acttctggaa ggaattcttc tactttcgtt ttgaaggtgt
     6061 cacagggcgc aaaataagcc tgaaagacga atcgaataca actgtgagtg atgccaatgt
     6121 cttagcaaat atcatcttag atttcatctt tgaacatcat attcctttca aagaaggcta
     6181 tgagatttta cctgcgaatc aagaatatta cttctacaaa tgcatcacaa aaagagtctg
     6241 ctgcatctgt ggcaaaacag gagctgacat cgatcacttt gacaaagcgc taggaagacg
     6301 aaagcgcaaa gaagttgatc atgcagagta cacatttgca gcactctgca gaatccatca
     6361 cacagagaag cacaaaatag gtgtgattaa tttcaaaaat aaatatcaaa tcaaagggat
     6421 caaattaaac cagaaaacaa tcaaaaagtt aaatattgga ggggaaaaat gacagaacat
     6481 cgaagttatt atgcgattat accagccaac gtaaggtacg acaaaagact taaaccaaat
     6541 actaagttgt tatacggaga gataacggcc ttgtgtaatg aaagaggctt ttgttgggca
     6601 ggcaatgagt actttgcaga tttatatggt gtgaataaag agaccatatc gcgatgggta
     6661 agtgatttga ttaagtttgg atacttgaat cgggaaatca tttacaaaga gggtaccaat
     6721 caaataatca ataggtacct acgaattaat caatacccta ttgacgaaaa acgcaatacc
     6781 cctattgacg aaaaagtcaa agataataat acatctatta ataatacatt taataataca
     6841 aaagaatata taagagagtt accgccttcg aaaaaatcga aggctaagcc cgtccgtcat
     6901 aaatacggag agtataaaaa tgttcttttg tcagatgagc agatggagaa actcaaaaca
     6961 gaattcccta atgattacca agagcgaatc gaacgactgt cagagtattg tgaatcatct
     7021 ggtaagactt ataaaaacta tttggcaact attcgaagtt gggcaaggaa agaaaaaagt
     7081 gaacctaaga acgcaagcag tggatacaag cgcacaggaa gacgagagaa gcttcctgaa
     7141 tgggcaatcg accaagaagc ctatctcaag aaaaaagcgc tagaacgagc taatagacaa
     7201 tcaaaagcac cattctaaga ggtggaaaaa tgaagatcga ttatctagaa ctaattaatg
     7261 aaatagcgaa ttataaaaag ggcgaggaat tagacgtcct gagagacgta tatgatcaac
     7321 tcgaagaagc tggaattgaa ggaattaaga atgatcgttc gagttggagt aaactcagat
     7381 actatttcgc actctatatc gatggaacgc aattaagaaa tttagcatat accaaattac
     7441 tattcattga ttgtgttaaa ggcttgcaaa aacatcttaa tgaacttgag caggtgtaaa
     7501 caagatggac ctaaagacat ttacagcaca gatcgaatta atgcatcaag aagctttaag
     7561 acaaagtgtg tcgtacgaag acaagtggct caacacattt catggcggac gtgagagcgc
     7621 acttgatcaa gtactcaaat tactgaaagg agaatgtcgg gatggataag aaagcggcaa
     7681 tgcagcgaat tatcgaattg acttattcag aagattggca ggaagataaa aaaatagttg
     7741 cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa gcccaaacgg agaacgccga
     7801 gaaagattgc aatctggcat ggtgatcgaa ttctagtaac gggtaccgct aaacagttat
     7861 ctgaaattac tggtctgagc aaaaatatta tttgggatag agctaggagc ttatggattg
     7921 attcaaaagg aagacagttt aggtatgtgg aggagaaata atggacgaac taatcgcgaa
     7981 agtagagcag tgggcaaaag ataagggatt ggatcaagcg gatttcagca agcaaatgtt
     8041 aaaaacggtc gaagaaatcg gggaagttgc cgcttctcta gctagaaaag atgaacatgg
     8101 tttaagagac ggaattggcg atgtagtagt aaccttgatc atcttggcaa tgcaaaatga
     8161 tatggattta tacgagtgct tggattttgc ttatgacgaa atcaaaggtc ggactggaaa
     8221 gatggtcaat ggtgttttcg ttaagtccag tgacctgaaa taattccatt aatggaagaa
     8281 ataactgaat aggaggatag acatgattga tgcaaaacaa gtgcaaaaac agaaagatgg
     8341 aatgttgatg tttgaagcat atgttctacc atttcttaat caatttgaag ttttggaatg
     8401 ctctgccagc ggagaagaat tggagtatgt tgtaattcgt gaaaccaaag aaaacgttca
     8461 gaagctaaac gaatttcttt gcactattaa ctgttgggat atgatagctc caggattttt
     8521 atgtccagct atgggagaat ttttggaata ttgtcgtctt gaagatgcag gcactttgga
     8581 tttagcttat ctggtataca actacctaaa catcaacaca gatcatttgt ggtttggcac
     8641 tgccgaaaga aaatgggtag tccgctaacc accaatatca atgaaaaaag gcggtttcct
     8701 gaaagtggaa ttgccgccat tgctgtaagg ttctttacgc gactcaaggt cacagaaata
     8761 taaatgcaaa tctcttttga agtgatccaa agctctcgga aaatggaagt tttttgtttc
     8821 aaagaataaa tgagatttgc gtcaagaacc tgagagttat ttccaaaaaa gcttccaaag
     8881 aaccgaatga gctaaggaca tcatgtgtaa acacataata tagctctcct ttcacggcta
     8941 atcataacat gaaaaatatt cgatgtcagt aggaaaaaag gtgaattatg aaaagaatat
     9001 taaattatcc cggcagtaac agattctgca attaaactcc gaagctgatg tatcagtatc
     9061 aactgtagaa tattatgagc gtggagaaag tcaagcagtc acaaatgtaa aaatcgataa
     9121 cgtcaacggt cttatctgtt tagatgaagg cgatagtatg aatgaaattg ctgcagacca
     9181 tgaattatca gaaactgctt taattgatca aatcgaatta gctagacaat actttgttcc
     9241 atatgcagat acctggtcca agcataagaa tgacatcatt tttccagagg aacaatgaga
     9301 gcaactgatc cagtaattat ccttgaggaa gccaaattta tttggactca cgaagagata
     9361 gagcaagcac gcttgctctt ttctcaagga gttaagccga gcaaagtagc tgaaataatg
     9421 ggtcaaaaga ttcttgatgt cggattgctt ttgctccatc tagcagaaaa aatttgattt
     9481 gaggtgaaaa tgatgattca acttgcaggc atccaaacag ggaaaattta tttttctgga
     9541 gaaagcaaaa gcgaagccaa ccaatggcta ttgaaaactt acacgaacaa taagaagcta
     9601 aggaaaaaat atcctgattt gttgctaaag gatgatcaga ttatgccgga accgatgatt
     9661 ttgacccgta aggagggaag ttgaaactaa aaaacca
//
LOCUS       DAYYAR010000105         9690 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000105 DAYYAR010000000
VERSION     DAYYAR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.106"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..641
                     /locus_tag="ACHAT0_002189"
     CDS             18..641
                     /locus_tag="ACHAT0_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452116.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1091..2140
                     /gene="trpX"
                     /locus_tag="ACHAT0_002190"
     CDS             1091..2140
                     /gene="trpX"
                     /locus_tag="ACHAT0_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8452117.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2137..3018
                     /locus_tag="ACHAT0_002191"
     CDS             2137..3018
                     /locus_tag="ACHAT0_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8452118.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3021..3791
                     /locus_tag="ACHAT0_002192"
     CDS             3021..3791
                     /locus_tag="ACHAT0_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8452119.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4070..4924
                     /gene="aroE"
                     /locus_tag="ACHAT0_002193"
     CDS             4070..4924
                     /gene="aroE"
                     /locus_tag="ACHAT0_002193"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8452120.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5102..6265
                     /locus_tag="ACHAT0_002194"
     CDS             5102..6265
                     /locus_tag="ACHAT0_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8452121.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6382..6792
                     /locus_tag="ACHAT0_002195"
     CDS             6382..6792
                     /locus_tag="ACHAT0_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8452122.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6966..9194
                     /gene="topB"
                     /locus_tag="ACHAT0_002196"
     CDS             6966..9194
                     /gene="topB"
                     /locus_tag="ACHAT0_002196"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8452123.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9365..9649
                     /locus_tag="ACHAT0_002197"
     CDS             9365..9649
                     /locus_tag="ACHAT0_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452124.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3361 a         1645 c         2083 g         2601 t
ORIGIN      
        1 aaaaagtaga aaagaaggtg tttgttattt gggaagtagt taaatcgaga gtacaagaaa
       61 tgaatgaaca tttctcagtg tcattcgcag aaaatccaag taagtatttt cgacgtcttg
      121 tttttctgct catcttatca ttgctgttaa cagttgcaat gtttttattg ccggaaaaaa
      181 tccgagaaga gcaagtaaat agtctcgttt attcgtccat accaaccagc aaattgaaac
      241 cttttgatta cagcaaagct gatcagaaaa tcaaacaaag ccgggcgata tcggtgatgt
      301 tcagcaaacc gaacgggaaa acctatcagg acatgcttga tgtttttaat gatcctgagc
      361 ttatggaaga attgaatcga ccgatctttt attatccgat cgtttatgat gtacatgaac
      421 tggaacaaaa atataacata cgaacggatg aagtgacatt tattttcttt gatggaggaa
      481 aagaagcaaa tcggataact gcgggtaagg gaggaataac tgattttgat aaggagttga
      541 tccctgaact gaatcgactt ccgttagcca atatcaaaca attagaagaa gaattggata
      601 gaacgggagt aacttcccaa aattccgaga ttgctaattg agacattggc gcgatgaaac
      661 ggtgggtcta tatagcgcta tttttaatta tcagaacaag tgatgtttga tcttcaaaat
      721 aacaaataaa aaattaaaaa atatagttga cgaaattcaa aaattcgatt agacttaaat
      781 caattcttaa ggaaagggtg acctgtaatg aacttatcaa aacgtatttc agcagaaaac
      841 aaaaaaaatt ggcgcagctg gcgtagataa gttgtattaa tggtgagact ttcccataga
      901 tacaactttt tagagtgcat ttctaaagct tgtatctatg ctagacatga acattgactg
      961 ccgcatagat tagaatctat gcggtttttt gtgtaaagac aatgaccgct ttggccattg
     1021 tctttttttg tagactggat acttgcctga ttataataaa taaaaagatt gactttgcag
     1081 gaggaaaaaa atgaaaaaca aacgattaat aacagttgta gtaattattt tagtttattt
     1141 agcaggtgca tttatttatg gaaaagcctc aggcgaaaaa gaaagtactt cgacgaacca
     1201 gacaaaaaaa acaacagttg ttggggtatt gcaatacgtg agccatccgg cattagatga
     1261 gatttatcga ggaatcaaag atggtctgaa agaagaagga ttaaaagaag gagaaaatct
     1321 tgagatcaaa tttcaaaatg gacaagccga tcagagcaag ctagccacaa tgagtcaaca
     1381 gctagtacaa tctgatccag atgccttgat tggaatcgca acacctgccg ctcaatcatt
     1441 agcaaacacg acttcatcga tcccagttgt gttaggtgct gtaacggatc ctgtgggggc
     1501 tggattagtc gcttctttag agaaacctgg tggtaatgta acaggtgttt ctgatcagcc
     1561 gcctgttact tcacaaatca agcttgcttc tgatttgttg ccaaatgcaa aaaaagtcgg
     1621 aatcttgtat gcttcttcag aggataattc aaaataccaa gtagcacaag cagaagaagc
     1681 tgcaaaaaaa gaagggttag aatcagtaaa atatgcggtg ccttccacta atgagattgc
     1741 acaaacggtt caagtgatga gccgacaagt cgattttatc tatgttccat tagataatac
     1801 cattgccaat gcgatgcaaa cagtggtaaa agaagcaaac aaagcaaata tccccgtcat
     1861 tccatctgtg gatacaatgg tggaacaagg tggactagca acaatcggga tcaatcaata
     1921 tcaattaggg cttcaaagcg gtaagatggc ggcagaactt gcgagtggaa aagaaaaacc
     1981 ggaaacgacg cctgtttata catttgatca aggggataca gtgatcaatc aatctcaagc
     2041 tgatcgctta ggaatcattg ttccccaatc gatgaaggaa aaagccaaaa tcattacaga
     2101 tgactcgcag caagaaacat ctaaggagga tgacaaatga tcgtatcagc tatttcacaa
     2161 ggcatgcttt gggcaatatt aggattaggg attttcatga cttatcggat cttagacttt
     2221 cctgatatga cgacagaagg gtccttccca ttaggtgctg ctgtctgtgt gacagctatt
     2281 actcatgggg tagcgccgat tgtagctaca ttattaggcg taggtgctgg catgatagca
     2341 ggactcgtta cggggttact ttatacaaaa ggaaagatac cggttatttt agcagggatt
     2401 ttagtgatgt caggattgaa ctcagttatt ttgtttgtga tgcgaacacc aaatctttct
     2461 ttattgaatc atccgattct acaagacagc ttccatcgct ttgggttacc tgattacttt
     2521 gacacagtcc ttcttggctt catctctttg ggcatcttga ttgctgtact gctattcttc
     2581 ttcaatacag atttgggtca aggctatatt gcgacaggag acaacgaaac gatggctcgg
     2641 tcgttaggga taaaaaccga tcgtatgaaa attttaggtt taactttgtc gaacggtgtc
     2701 attgcattat ccggtgcgct gattgcccag aacgacggat atgcagatgt aaataaaggg
     2761 attggcgtaa tcgtcatcgg tcttgcttcc atcatcatcg gtgaagtgat cttccaagaa
     2821 ttgacgctag cagaaagatt gattgctatt gttgtcggca gtatcattta tcagctattg
     2881 attttagtag taatcaaact agggtttgac acgacttatc tgaagttgtt ctccgctatc
     2941 atcttagcta tttgtctgat gatcccgcaa ttgaagaaag cactgaactt aaaaacgggt
     3001 tttgagaagg aggcatagta atgactgata tattgacaat caaaaatggc agaaaagtcg
     3061 taaacaatgg aatgaatgaa gagaaagtac tattggatga aattaacctt acaatcagag
     3121 aaggggactt catcactgtc cttggcggga atggtgctgg aaaaagcaca ctgttcaata
     3181 cgatcgctgg tacgcttcaa ctcagtcaag gatcgatctc ttttaaagat cagctgatca
     3241 caaaagattc agaagaaaaa agagcacgat tcctttctcg agttttccaa gaccccaaga
     3301 tgggaacggc tcctcggatg acagtagcag aaaatttgct acttgcacaa aaaagaggtc
     3361 aaaaacggaa attccgttta cgtaacttga aaaatcaaaa agaagatttt ttcagactat
     3421 gtcaagaagt cggtaatggc ctagagcaac acttggatac tccagcgggt gaattatctg
     3481 gtggtcaacg ccaagcatta agcttgctga tggcgacgat cacagctcct gagttactat
     3541 tattagatga acatactgca gctttagatc ctaaaacagc taaagcatta atgcgattaa
     3601 caaatcagcg gatcaatgaa caaaaattga cttgtctcat gatcacgcat cgaatggaag
     3661 acgccttgaa atacggaaat cgtctgatcg tactccaaaa agggaaaatc gttcaagata
     3721 tttctgaaaa agaaaagcaa catttgacaa tgggcgacct tttgcagttc tttgaagaag
     3781 tagtggattg atttcgacag aaaaattcct ttcagtagaa ggacttgaaa actcattgac
     3841 atcttccttt gattcatcct atacttaaaa caataaacaa ttaaacatat ttgggtgttt
     3901 tgtaggatca cgtagtcaat gattttaaca attaaataga atattctttg ctctgacaag
     3961 taaacgatga gaaaagaatt tgctactttg ccacctagac gtttcgtact ttttcgtatc
     4021 aaacgctaag tggctttttt tattgaagta agaacaaagg aggaataaga tgatttctgg
     4081 acaaaccaaa ttagctgggc tctttgctga tccggcttct cacagtttat ccccattgat
     4141 gcacaatact gcttttgaag caaacaacat cgatgcggtg tatttagctt tccaagtaaa
     4201 tccaatgaat ttgaagcaag cagtcgaaag tattcgaact tttgatatgt taggtgtaaa
     4261 tctttcgatg ccgaataaaa cagcggttat tccttatctg gatgaggtaa gccaggaagc
     4321 gcaattgatc ggagcagtga atacgatcgt gcacgaaaag ggtcgtttga ttggctataa
     4381 taccgatggt gcgggcttca tgcaatccgt gcaagaagct gggatttcga tcaaaaagaa
     4441 aaaaatgact ctactgggag ctggaggtgc agcaaaggca gtcactgttc aagctgcgtt
     4501 agacggcgct gaggaaatcg tagtttataa acgaaacaac caaacattcg ctcaagtaaa
     4561 agaaacattc gaacaactca gctatcgaac ttcttgtaag ataaaagttc gtgactttgc
     4621 cgatacgcat caattaagaa aagacttgaa agaaagctgt cttctagtca atgcaacaga
     4681 tatcgggatg ggaaagaaaa tcaatcagtc accgctctta gatataagca tgatgcatga
     4741 gaatctagcg gtttttgatt tgatttatgc acctagagaa acacgtttat taaaagaagc
     4801 aaaaaaagta ggagcaaaag gatacaatgg acttggcatg ttgatctacc aaggagcaat
     4861 cgcgtttgaa ttatggacaa aggaaaagat gccgatccat gtgattgaaa agctgtttga
     4921 ttaaaaaagg ataatagata aagatctttt attctcgcat aaacggtctc aagaaaagta
     4981 atcctttatg tatcgaagca tttatttcgt tattttttat taaattatca aaaaatgata
     5041 ggcttttgtc gattggttat cgtatccttt atttatcagt aagaaacgag gaggaaccac
     5101 aatgagtcgg atggatcgct ataaggacat ccataagaag gcgaaacctc tgaagcggga
     5161 tacggggagt atttttgcta gaagagaacg aaaacgaacg cgtatggaaa atgaggaaga
     5221 tgctacccgt atttatgaaa gagagcagca ttcatttgaa catttgaacc aatacgagca
     5281 acagatatac agagatgcac caagagcaga agaacaacaa aaaaataaaa aatttttcaa
     5341 tcggcaaaag aagaacaaac cgttaaaacc tcgaagaaag cgatcgtgga aaaaaatcat
     5401 tctatcgatt ttgcttgttt tgatccttta ttcaggtatc tcatttttta ttggtaaata
     5461 tgtagctgaa catgacacaa gcttagaggc agcgacttca gaaacctttc atgggcaaaa
     5521 agcgacgaat ggtgcgcata atattttgat ccttggaagt gatacacgcg gagaagatgc
     5581 cgggcgagcc gatacgatca tggtcttaca gttggatggt ccttcccata agccgaaact
     5641 gatttctttt atgcgtgaca gcttcgttac gatcccaggg gtaggacaaa ataagatcaa
     5701 ttcagcttat gcatacggag gagccgatct agtgcgtcaa actttagtag aaaattttgg
     5761 aatcgattgt caatactatg cgaaagtcga tttcaaatcg tttgaaaaag tgatcgatgc
     5821 cttattcatg aacggagtga agatcgatgc agaaaaagat ctgaatctgg atggtgtaga
     5881 tattaaaaaa ggggtccaga agatggatgg acatgttctt ttgcagtacg cacgtttccg
     5941 aatggatgaa caaggagact ttggccgtgt cagacgccaa cagcaagtaa tgaacgctat
     6001 tttcagccaa ttgaagaatc cattgaatct gatccgtgca ccttatgcag ctggtaaagc
     6061 tttaggatat acatccacag atgtcactgc ttggttcttg acgaaaaacc tcttttcgat
     6121 tgcacgagga gctggtggca ttgatcgaat aagtgtgcca gtagacggtt cgtggaatta
     6181 tgggcaaagt gattatgctg gcagtattct tgaaattgat aatcaaatga atcaatctgc
     6241 tattacagat tttttgaata aatgatcata aaaaagatca agaagaagct gcgcaaggct
     6301 tttgtcatga atcaatcaaa cgattcactc gtaaaaaacg ctttcttatg gtagatttaa
     6361 ccaagagagg agctgtttac attggatatc tatctgaact tcaaaaatga gtcgcgtgaa
     6421 gccatcgctt tttatgaaaa agtttttcag actgaagcat caggtatcac cacatacggt
     6481 gaaatgcctg gaatagaaca aatgaacttg ccagaagaag caaaaaatct aatcacgaat
     6541 gcacggctga acatttatga tacattagtt atgtttgcag atacgccaga aggaattggt
     6601 cctgaattag ttgtgggaaa taatgtttct ttagtcatcc agacaagaaa tgaagaagaa
     6661 acgaagcgcc tatttgaaag cttagctgag ggtggtaaga ttgagatgcc actggatgct
     6721 gttccgtggg ctcagttgta tgggatggta actgatcgat ttggcatcaa ctggcagatt
     6781 aaccaagagt gacagctaaa gatgaatatt ttctggaaac atcaagcagc aaacggatca
     6841 tcgtttgttt gcttgatgtt tttgtgtcta tttcgactat acggtatctt ttttttattg
     6901 ccagatgcga aaatatgttc cataatagag aagtccgcaa aaaattaagt aacggaggtc
     6961 agaagatgaa gacggtgata ctagctgaaa aaccttcaca agcaaaagcc tatgccgaaa
     7021 ccttccaaca gcaaaagcgg catgaagggt attatgaaat cagcgatcca ttgtttgctg
     7081 gtgaagtaac catcacttat ggttttggac atttggtaga tatggttgct ccaggagctt
     7141 atgaagagcg ttgggcaaaa tggtctttag aaaatttgcc tattttccca gaaaccttcc
     7201 gttacgaagt tccaatggac aaacgtgcac aattttcgat cgtaaaacga gaattgcagt
     7261 cagccgatac catcattatt gcaacagatg gtgatcgaga aggtgaagcc atcgcgtggt
     7321 cgattattcg acaagcaaac gcattttcta aggaaaagaa ctatttacgt ttatggatca
     7381 attctcttga aaaagaagca atctatgaag gttttaagaa tctccaacct ggaaagaact
     7441 attttccaaa atataaagaa gcgcaagcaa ggcagaacgc tgattggctg attggaatga
     7501 atggaagtcc attatatagc ttgctacttc aacgaaaagg gattgatggc agtttttctc
     7561 ttggtcgtgt acaaacaccg acactctata tgatttatca gctgcaagaa gaaatcaaac
     7621 acttcaaaaa agaaacgtac tttgaaggag aaggtttaat tacgacaaac aaagggcagt
     7681 tttctggaaa aatcgtacca aaaaaagcat ttaaaaccca agaagagtta acgaaggaaa
     7741 tagaaaaact tggagcgcat ttaggtaagc aaaacggacg gattcttgaa gttacgaaaa
     7801 aggaaaagag gttgcatagt ccacgtttat tttcgctatc tagtttgcag acaaagatga
     7861 atcagctaat gaaagcaagt gccaaagcta cacttgatgc tgctcaagga ttgtacgaaa
     7921 gcaagtttct aagctatcca agaacggatt ccttttacat tacagaaaat gagcatcagt
     7981 atctggttaa acatctgcaa aattataaaa aatttttagg aatagaagaa gtacagacaa
     8041 ctgagatcaa agcaaagaaa cgatatgtcg atgcgaaaaa agttcaagaa catcatgcaa
     8101 ttattccgac aaagacgatt ccgactcctg cacgctttgc gaagctttcc aaattacagc
     8161 aagcaatcta tttgcaagtt ttgcgcacta ctgtggctat gtttgctgca gattatatct
     8221 acgaagagac gaccgtaatg acgggcgtac agcaattaaa gctaaaaagc atcggaaaga
     8281 tcccattaca gcaaggttgg caggaaatct tacgcatcaa gtcaaaaaag aaagaagctc
     8341 agactcttcc tcctatcgta gaaaatgaag aagcagaagt agaactgaaa agtatggaga
     8401 aagaaacaca acctccaaaa ccctataccg aaggaacgtt gatcacagca atgaaaactg
     8461 cgggaaaaac gctggataat gatgaagcgc aagaaattct caaagaagta gaaggaatag
     8521 ggacagaagc tacacgagcc aatatcattg aaaatcttaa gcagaagcat tacatcgaag
     8581 tcaataaaaa cgaaataact gttacgccaa aaggaatcac tctttgtaag gcagtagcca
     8641 atgaaccatt actgacgagc gcagaaatga ctgcgcgctg ggaagggtac ttgagaaaaa
     8701 ttggtaaaca agaggggaca ccatctgttt ttttagagaa tatcaaaaaa ttcatcctac
     8761 atttattggc agaagttcct aatcaaatcg acaaggtaga cttttcagat gagattagcg
     8821 ggacacgtca gataaaagcg atggagaaga aaaacgatca attaggaaga tgtccaaaat
     8881 gtaaaaaggg aatagtcatg ctttatccta aaatcgctac atgcttgaat ccggaatgcg
     8941 acttcaaatt gtggccaaca gtagctaaaa agaagctgac taaaacgaat ctcagagatt
     9001 tgctttcaaa aggaaagaca agtaaagttg tcaaaggttt tactggtaaa aaagggaaat
     9061 tcgatgcagt tttagtatta aaagaagata tgacaatcgg tttttctttt ccatggatcg
     9121 aaaacagtgg aacaaaagca gttgatggaa acaaagaaac atcaagaaaa aataatgaaa
     9181 acgaagtaga gtaaatgaaa acaatgaaat agcggaatat ttattccgaa aagtgagaat
     9241 ataaatacca ataaaactaa aaaatatcag tgaatcaagc aaaaacgttg gggtaggctg
     9301 attttatata gatagcaagg agttttttta tttgaaaaaa gtgcattcat ttttaaatta
     9361 tcttatggga gcaatcacga ttgtggctgt gtaccaagca tttctaaagt atcctgatta
     9421 cgggacgaag ttgttgtcat tgacgcacaa tcaaacagca tattcaccaa cgttgatttt
     9481 atcgactttg actgcaggag ctaaaagcgc tacttatttt tttcataaaa cgacaagcat
     9541 gctaaagcga aagaaacagc gaaaacaatt gttggtggtg gtagcggcta tgatcattat
     9601 ttattttcta ccagttatta cacaagtatt tcatcaatat tttggttaag aatacgagag
     9661 tttgtgacaa aagtcatgtc acaaactttt
//
LOCUS       DAYYAR010000106         9553 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000106 DAYYAR010000000
VERSION     DAYYAR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.107"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..417
                     /locus_tag="ACHAT0_002198"
                     /pseudo
     CDS             1..417
                     /locus_tag="ACHAT0_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(421..1140)
                     /locus_tag="ACHAT0_002199"
                     /pseudo
     CDS             complement(421..1140)
                     /locus_tag="ACHAT0_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(1091..1300)
                     /locus_tag="ACHAT0_002200"
     CDS             complement(1091..1300)
                     /locus_tag="ACHAT0_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452125.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(1282..1350)
                     /locus_tag="ACHAT0_002201"
                     /pseudo
     CDS             complement(1282..1350)
                     /locus_tag="ACHAT0_002201"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            1577..2850
                     /locus_tag="ACHAT0_002202"
                     /pseudo
     CDS             1577..2850
                     /locus_tag="ACHAT0_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2957..3988)
                     /locus_tag="ACHAT0_002203"
     CDS             complement(2957..3988)
                     /locus_tag="ACHAT0_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8452126.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3995..4825)
                     /locus_tag="ACHAT0_002204"
     CDS             complement(3995..4825)
                     /locus_tag="ACHAT0_002204"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8452127.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4822..5637)
                     /locus_tag="ACHAT0_002205"
     CDS             complement(4822..5637)
                     /locus_tag="ACHAT0_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8452128.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5634..6458)
                     /locus_tag="ACHAT0_002206"
     CDS             complement(5634..6458)
                     /locus_tag="ACHAT0_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFM8452129.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6448..7548)
                     /locus_tag="ACHAT0_002207"
     CDS             complement(6448..7548)
                     /locus_tag="ACHAT0_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8452130.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7726..8511
                     /locus_tag="ACHAT0_002208"
     CDS             7726..8511
                     /locus_tag="ACHAT0_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8452131.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8544..8927
                     /locus_tag="ACHAT0_002209"
     CDS             8544..8927
                     /locus_tag="ACHAT0_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8452132.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9003..9134
                     /locus_tag="ACHAT0_002210"
     CDS             9003..9134
                     /locus_tag="ACHAT0_002210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8452133.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9103..9339
                     /locus_tag="ACHAT0_002211"
     CDS             9103..9339
                     /locus_tag="ACHAT0_002211"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8452134.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            9351..9437
                     /locus_tag="ACHAT0_002212"
                     /pseudo
     CDS             9351..9437
                     /locus_tag="ACHAT0_002212"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(9417..>9553)
                     /locus_tag="ACHAT0_002213"
     CDS             complement(9417..>9553)
                     /locus_tag="ACHAT0_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM8452135.1"
                     /translation="IELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF
                     "
BASE COUNT         2796 a         2268 c         1850 g         2639 t
ORIGIN      
        1 gttccatgag ttttatttgt gttgatggga caacccatca gctatttacg atccttgaag
       61 atcgcagact ttacaaacta actcaatact tcctgcactt tcctagaaaa gcccgtttaa
      121 aggtcaagta cctagaatgc cagctattgt cagctgctta aaacggtctt tccaaatgcg
      181 gagatcgtaa ctgatcgctt tcatattgtc caacatatca atcgcagctt caatcaattg
      241 cgtatccaga tcatgaatcg ttttcgagcc tcgcactctg aaaatcagaa gaagtaccgt
      301 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
      361 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
      421 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
      481 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
      541 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
      601 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
      661 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
      721 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
      781 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
      841 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
      901 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
      961 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
     1021 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
     1081 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
     1141 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
     1201 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     1261 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     1321 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     1381 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     1441 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     1501 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     1561 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     1621 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     1681 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     1741 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     1801 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     1861 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     1921 ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt ctaaacattg
     1981 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     2041 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     2101 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     2161 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     2221 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     2281 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     2341 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     2401 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     2461 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     2521 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     2581 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     2641 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     2701 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     2761 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     2821 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     2881 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     2941 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     3001 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     3061 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     3121 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     3181 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     3241 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     3301 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     3361 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     3421 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     3481 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     3541 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     3601 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     3661 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     3721 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     3781 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     3841 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     3901 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     3961 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     4021 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     4081 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     4141 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     4201 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     4261 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     4321 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     4381 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     4441 acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc gttaccaaaa
     4501 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     4561 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     4621 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     4681 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     4741 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     4801 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     4861 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     4921 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     4981 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     5041 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     5101 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     5161 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     5221 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     5281 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     5341 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     5401 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     5461 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     5521 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     5581 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     5641 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     5701 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     5761 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     5821 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     5881 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     5941 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     6001 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     6061 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     6121 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     6181 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     6241 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     6301 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     6361 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     6421 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     6481 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     6541 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     6601 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     6661 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     6721 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     6781 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     6841 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     6901 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     6961 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     7021 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     7081 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     7141 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     7201 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     7261 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     7321 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     7381 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     7441 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     7501 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     7561 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
     7621 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
     7681 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
     7741 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
     7801 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
     7861 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
     7921 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
     7981 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
     8041 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
     8101 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
     8161 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
     8221 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
     8281 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
     8341 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
     8401 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
     8461 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
     8521 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
     8581 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
     8641 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
     8701 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
     8761 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
     8821 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
     8881 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
     8941 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
     9001 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
     9061 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
     9121 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
     9181 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
     9241 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
     9301 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
     9361 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
     9421 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
     9481 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
     9541 aaaagctcta ttc
//
LOCUS       DAYYAR010000107         9432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000107 DAYYAR010000000
VERSION     DAYYAR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.108"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..680
                     /locus_tag="ACHAT0_002214"
     CDS             <1..680
                     /locus_tag="ACHAT0_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8452136.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="ACHAT0_002215"
     CDS             800..1000
                     /locus_tag="ACHAT0_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8452137.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="ACHAT0_002216"
     CDS             1076..1273
                     /locus_tag="ACHAT0_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452138.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="ACHAT0_002217"
     CDS             complement(1570..3117)
                     /locus_tag="ACHAT0_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFM8452139.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="ACHAT0_002218"
     CDS             complement(3201..4004)
                     /locus_tag="ACHAT0_002218"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8452140.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACHAT0_002219"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACHAT0_002219"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM8452141.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACHAT0_002220"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACHAT0_002220"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFM8452142.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACHAT0_002221"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACHAT0_002221"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFM8452143.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="ACHAT0_002222"
     CDS             complement(6389..7318)
                     /locus_tag="ACHAT0_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8452144.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="ACHAT0_002223"
     CDS             7513..8196
                     /locus_tag="ACHAT0_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFM8452145.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="ACHAT0_002224"
     CDS             8357..9400
                     /locus_tag="ACHAT0_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8452146.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DAYYAR010000108         9257 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000108 DAYYAR010000000
VERSION     DAYYAR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.109"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACHAT0_002225"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACHAT0_002225"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8452147.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="ACHAT0_002226"
     CDS             1286..2461
                     /locus_tag="ACHAT0_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8452148.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="ACHAT0_002227"
     CDS             2465..3106
                     /locus_tag="ACHAT0_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8452149.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="ACHAT0_002228"
     CDS             3103..4020
                     /locus_tag="ACHAT0_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8452150.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="ACHAT0_002229"
     CDS             4017..4688
                     /locus_tag="ACHAT0_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8452151.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="ACHAT0_002230"
     CDS             complement(5229..5717)
                     /locus_tag="ACHAT0_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8452152.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="ACHAT0_002231"
     CDS             complement(5918..6184)
                     /locus_tag="ACHAT0_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8452153.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="ACHAT0_002232"
     CDS             complement(6184..6447)
                     /locus_tag="ACHAT0_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8452154.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6968..7108
                     /locus_tag="ACHAT0_002233"
                     /pseudo
     CDS             6968..7108
                     /locus_tag="ACHAT0_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7250..9073)
                     /locus_tag="ACHAT0_002234"
     CDS             complement(7250..9073)
                     /locus_tag="ACHAT0_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8452155.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         2757 a         1947 c         1764 g         2789 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat caggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacattt
//
LOCUS       DAYYAR010000109         9237 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000109 DAYYAR010000000
VERSION     DAYYAR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.110"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACHAT0_002235"
     tRNA            2..74
                     /locus_tag="ACHAT0_002235"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACHAT0_002236"
     tRNA            78..150
                     /locus_tag="ACHAT0_002236"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACHAT0_002237"
     tRNA            155..225
                     /locus_tag="ACHAT0_002237"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACHAT0_002238"
     tRNA            238..311
                     /locus_tag="ACHAT0_002238"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACHAT0_002239"
     tRNA            323..394
                     /locus_tag="ACHAT0_002239"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACHAT0_002240"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACHAT0_002240"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8452156.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACHAT0_002241"
     CDS             1983..3455
                     /locus_tag="ACHAT0_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8452157.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACHAT0_002242"
     CDS             3538..4326
                     /locus_tag="ACHAT0_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8452158.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACHAT0_002243"
     CDS             complement(4393..5061)
                     /locus_tag="ACHAT0_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8452159.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACHAT0_002244"
     CDS             5176..6888
                     /locus_tag="ACHAT0_002244"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8452160.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACHAT0_002245"
     CDS             6891..8663
                     /locus_tag="ACHAT0_002245"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8452161.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..>9237)
                     /locus_tag="ACHAT0_002246"
     CDS             complement(8745..>9237)
                     /locus_tag="ACHAT0_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8452162.1"
                     /translation="GLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRT
                     IELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDK
                     KVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGK
                     IEI"
BASE COUNT         2774 a         1552 c         2017 g         2894 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtatctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatatg cttgaataga tatcgggtta
     9001 ccatttttag agatgaaaat gtatttatct ttattattct taattaattt gttttcttca
     9061 attaattgat ctatgatttc tttagcatta cttgataatt caatggttct ataagaacct
     9121 gtatttttag ttgttgtttt aatttttaac tcgtaatcat aagtaccaga aaccgtaatt
     9181 ttattaccat cataatcaga ttcagtcaaa gatatcaatt caccataacg tagtcca
//
LOCUS       DAYYAR010000110         9080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000110 DAYYAR010000000
VERSION     DAYYAR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.111"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..416
                     /locus_tag="ACHAT0_002247"
     CDS             60..416
                     /locus_tag="ACHAT0_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8452163.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            406..672
                     /locus_tag="ACHAT0_002248"
     CDS             406..672
                     /locus_tag="ACHAT0_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8452164.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            831..1832
                     /locus_tag="ACHAT0_002249"
     CDS             831..1832
                     /locus_tag="ACHAT0_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8452165.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2202..2816)
                     /locus_tag="ACHAT0_002250"
                     /pseudo
     CDS             complement(2202..2816)
                     /locus_tag="ACHAT0_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3056..3301
                     /locus_tag="ACHAT0_002251"
     CDS             3056..3301
                     /locus_tag="ACHAT0_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452166.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3399..3620
                     /locus_tag="ACHAT0_002252"
     CDS             3399..3620
                     /locus_tag="ACHAT0_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452167.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3942..4622
                     /locus_tag="ACHAT0_002253"
                     /pseudo
     CDS             3942..4622
                     /locus_tag="ACHAT0_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            4762..5301
                     /locus_tag="ACHAT0_002254"
     CDS             4762..5301
                     /locus_tag="ACHAT0_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452168.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            6321..7490
                     /locus_tag="ACHAT0_002255"
     CDS             6321..7490
                     /locus_tag="ACHAT0_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8452169.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            7963..8517
                     /locus_tag="ACHAT0_002256"
     CDS             7963..8517
                     /locus_tag="ACHAT0_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8452170.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            8573..>9080
                     /locus_tag="ACHAT0_002257"
     CDS             8573..>9080
                     /locus_tag="ACHAT0_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452171.1"
                     /translation="MKITEFSEVFKTFSNIYNRIPEIEQNVVVDNDYYIENDFNKPRK
                     YDNLYNFIHNRTTINNNSNTRVLINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAI
                     NNGNLPSNKKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIF
                     ASIHDYIGL"
BASE COUNT         3391 a         1201 c         1579 g         2909 t
ORIGIN      
        1 taattcgaat ttttatgtta tatactataa tataaaatca atatatgata taattgggta
       61 tggaaaaaat atcttttgaa acatttaaaa gacctaatgg tcatgatgaa tttttagaat
      121 ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata gaagaaacag
      181 aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta gacactaatt
      241 tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agctatctat ttccatgtgg
      301 tcaatggaag gtatgtcatt acccatggat ttactaaaaa aacggagaaa acaccaataa
      361 gtgaacttaa acatgcaaaa gaacttagag aggagtggtt cacaaatgat gattaaaaaa
      421 ttgattgaag aaaagcaaaa agacaaagat tttgcagaag catatgaccg tgaaggaaga
      481 aaactcgaaa ctgcggttgc tctttatcat gcacgggagg aagcaggcct aactcaagcg
      541 gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga gagaggggat
      601 aacgtatctt tcgataaact tgatgatatc gctagggcat taggcaaaaa gctggtagta
      661 gaatttgctt aaaagcgagt tgaaaatata cctgtaatag gctcctaact aatcaattag
      721 tttagtgggc ctttttgtac taaatagata aaagttgtcc tatagtgtaa aagtcaataa
      781 attttataca aaaattactt taaagagaca taaagagaaa ggtacaataa atgaaaacca
      841 taaatctaat aaaagtattt atagcatcac caggtgatat agcagcaaag aggaatgaag
      901 tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa gtaattttga
      961 tgccaattcg atgggaaacg aatgcgattc ctacttatat aaataatagc tctgggcaaa
     1021 gtgttattaa taaacaaatt gtagaaaata gtgatatatt attagcgatt tttggccata
     1081 agttgggttc tccagttgat aattatagat cgggaactat agcagaaatt gattactttt
     1141 ataaaaatag caaaggcgag gtaggggttt ttttcttaga tgaaccagtt ccacaagttt
     1201 tggctgaaga gtatatagaa gttaagaagt acagggactc tttagaagat aagggttttt
     1261 atggaacata tagcaaagac aatatcagaa ggtttctcac aaaaaaagtg aattatttaa
     1321 ttgagaaagc acaaaataat ttagaatcaa aacgctttga tttagattct atttcttaca
     1381 agacaccttc agtaattaac aaaaaagtta ccggaactga taaagatttt gatgacaact
     1441 tagatatttt tgatccaata gaatttgaca atgatgaaag attgctgcta atatatgcgt
     1501 ttatggatga aaattctatc ttttcaactg ataattcaac ttatagaaaa gtagttcaat
     1561 gggaaagtga taacaattta aggagttact tgagtgatag agtatttgat gtactagaaa
     1621 aattaagtaa atcgggaatt ttaaaggaag aatacgattc ttcttttgat gagtatccat
     1681 cacgctatat tttgatttca gaggactata agaaatttaa aaggtatttt ttaaaaaatg
     1741 aaaaaaagat atctgttatt atggagaaat acaaagctcc aatacaggct gatccatttt
     1801 ccaatttgga tgaatcagat ttaccgtttt aaattttaaa tagataattt cattttttag
     1861 tagcagaggt ataaactttt ttaatgtttg aattgaaaag aaatgctaat aggtcgactt
     1921 tatgtttctc ttgtgcaatc gtaaatgcgg gggaccataa aattgatcta ttatcgagtg
     1981 tgtataaaat aaaattcaaa tcttattagt ttacataaga tacattatac taagttgtca
     2041 atgttaaatg cctttaaata aggacgattt atgcatcaat tatatttatg ttaactaaat
     2101 tctttttaac ataaatgtaa tcaacttagc tagatgaatc taaaatgatt gtctgtattg
     2161 atgacaaagc taattaataa ttacgtgatt caaaaaagaa atcatacaat tagttggtcg
     2221 atttcttttt ttattcggta aattgtttgc ctagtcacat tgtagtctgt tgcaatcttt
     2281 gagatagcaa ccccattctc aagatcctga acgatactcc tatagacaag gcgacgttga
     2341 ggatctttgg tgtctgcact gtataactta ggacgacctt tatagacgcc gtttgcttta
     2401 gcgattttaa ttccttgtgc ctggcgtcgt ttcgattcag tacgttcttg ttcagcaatc
     2461 atggctgaga tttaaatgat taagtctttg atgaatttat ctaggagagg atttccaatt
     2521 gcttcagcca tgatcggtaa actggtgata ataagtcgca cattcttctt ctttagataa
     2581 ttcactgaat cgataatctc gtcataattg cgtccaagtc gatcaatggc ttctacaata
     2641 aaaatatctt gctctctgac aaaatctaaa gcttcctgga aaatcggccg atgactaatc
     2701 gtcgctccag attgtttttc gataaaaatt ttttcagcgc caaacttttt catttcttca
     2761 atttgacgtt gttcattttg atcgatggat gaaactctaa tataagcaat tttcaaattt
     2821 attccctact tttctataac gtttataata caccctaatc atacgttaga actctaaatt
     2881 tttcaatgtt atatttttgt ctatttaggg taaactctaa atagacaaac ggtttttgaa
     2941 ttctaaaagt taaatagttt ataatagaag aaaaagaatg gggtgacgat ttcttagatt
     3001 gaaaaggaaa gataaatatt atagattatt acttttgaca gctgaagcag gatggatgga
     3061 ttttccagaa gttgtaaaag aagttcaaga gataggtgca actatttctg atagccgctc
     3121 ctctcctatg aactatcagg atgcccatgg aattaaaata attgatcaag agagaaatgt
     3181 cagaaaatac acaacaataa atgagtgtgt aattaatgaa aatctttgta gggcaccgat
     3241 aacgaaacac attaagaata gaagcaaagc aaaagacgga agaactttta ctaccttcta
     3301 agtttttttc atatttgttt gctatactta gatctaagga attagacttt cgctgcccat
     3361 tatttaagtt tgaggtagcg tcttgcgagg gatcaataat ggaagaagaa aaaatatttg
     3421 aaaaacgctg ggaattggct tcagttgaac aaagagcaag gtaccataat ctaatgtcct
     3481 cttaccgtaa tattgattgg acttacaaag aaaagaagta cttgctttgg ttatgtcagt
     3541 tagatgtaaa tacctttgaa acatttgaag tgattctaga taagattaaa aacagcaatg
     3601 aaaaaagagc tgatctataa tggttcgctc tttttatttt attaactctt tggttactat
     3661 atgtaaaata cagcttcata ttacaataaa tttcaggata caaatttatt taagcatata
     3721 taaatataat tgaaatagga cataaatata tgagtatgag aaaggtgatt ccatgtttaa
     3781 taaatgggca aaccacccaa tgaactcgag aggtagagat aatttgaagg agacggacaa
     3841 gttctgggaa gaagtatata gtaaaaacgg ttctgttgca aagttttcca aaaaatctat
     3901 tttagtgtaa aattgagaaa aaagacagag aggacagagt aatgaatcat tttaaaggca
     3961 aacaattcaa aaaagacgtc attattgtcg ctgttggtta ctacctgcgt tacaatctaa
     4021 gctatcgtga agttcaggaa ttgttatatg atcgtggaat aaatgtttgt catactacga
     4081 tttatcgttg ggtataagag tacagcaaag tcctctatta tctttggaag aagaaaaata
     4141 gacaatcctt ctattcatgg aaaatggacg aaacctatat caaaattaag ggacgttggc
     4201 attatcttta tcgtgcaatt gatgcggacg gcttaacctt agatatctgg ttacgaaaga
     4261 aacgggatac gcaagcagcc tatgctttct taaaacgact ccataaacag tttggtgagc
     4321 cgaaagcaat tgtgaccgat aaagcacctt ctcttggctc cgcctttaga aagttacaga
     4381 gtgtgggttt atatactaag acagagcacc gaactgtgaa gtatcttaac aatttaatag
     4441 aacaagacca tcgacctatt aaacgacgaa ataaatttta tcaaagtctc cgtacagcct
     4501 cttccacgat taagggcatg gagaccattc gaggaatata taaaaagaac cgaagaaatg
     4561 gaacgctctt cggcttttcg gtgtctactg aaatcaaggt attaatggga ataacagcct
     4621 aagatatttg gagttcagag agggcgcgtt tgattttcaa acttcgcaac agaaccgaga
     4681 accttgggtt ttcaagtgct ttttggttca ttaatcttaa ccatagtgtt tttaaagtgg
     4741 aaaacaagca acaaaaaaag tatgtggagc cttgtaggaa tagttcttat tatttttact
     4801 gtgactgcag caacagaagc aaattattcg cataaaattc agtcgtataa aggtcgttta
     4861 gttatgacca aaaaagatgt tacacgttat acatcctcaa gaactggagg cctatatgac
     4921 tatgcaccat tagatgcatt gaaatataaa gatcatttaa agaaacatga agtaaaagtt
     4981 ggtgatcggt gggagaatgc atctcataaa gcatataatt ccaaaatagt ctatacagta
     5041 gaagatgccg atgagcaaaa tatcacatta cctgtattca attattgggg aacagttgtg
     5101 aaagtaaatg ggaaaaagag cactatagaa aatgacaatg gtttagtgtc tattaaaggt
     5161 acggggaaaa gaactatagt tgaggtttca tctacgtatc caactacgat gattctatct
     5221 ttattaatta gttcaataac ttttatattc ttatttacac gttatttaat aagatgctgc
     5281 ggtgaaatga aaatagatta gatagatatt ataaatatgt tgattctatt ttcatagcaa
     5341 tggagaaaat acattagcaa tatataaaag aaggtatcaa gagtcacagt gaatttattc
     5401 cgaaaagtga aaagagcaaa tataaaattt aatgaaaata tagaactaaa taggaaaacg
     5461 tagacgtaat tttctacgtt ttccttttca tatatattaa atattacgga aaattatata
     5521 aactaattta gtgttttgtt tgtaattcct aattttgctc cacttttaga taatttttag
     5581 gtctatttaa ctcatcagac attctatgct catttttctc caaatttgtt tcaactcttt
     5641 cgtacttaat tttgcgtatt tgaaaattct tcttttagag tacttttatt acgatactag
     5701 tatgatatat atctatagca aacagttaaa atcttcctaa aataagcgaa tagcattcct
     5761 aaaacctata aaatttttag atgaagcgac aagtggacaa ttaataaact acttgaacat
     5821 gaaattatgg atcacctttc aaatttagaa aatggaattc ttatacttat tgcgcaacat
     5881 ttattgattg caaaattatg tgacaaaaaa aactagtaga agaaagaata atggcttgat
     5941 aataaagtac tacctattaa aggtagtact ttatttaatt tagtataaat taacatacta
     6001 ctatatttta ggtttcatat tatataaatt aataatatac taccatttgt tggtagtata
     6061 atttaaaggt aataagttat tcttttatta ctatgtttgg gttttatagc aaagaaagtt
     6121 taataagagt ttggacctag caaacaatat taatgaggtc attaaatctt attatcttta
     6181 gtgatataac gaaatatcta cacagacaaa atgatagcgt aaaatatttt gtgtaaatag
     6241 atatttaggg tagaaaaaaa gaaagtccat tctgtaaaat taaagttacc acaaccgaaa
     6301 ttttatagga ggactttctc atgcccaatt ttactacaga aattatggaa acactaatca
     6361 ataaaggtga tttagatgaa ttatttcgat gtcatttaga attagctgtt aattcattat
     6421 tgcaggctga attaacagct tttcttgatt atgaaaaata tgatcgtgct gggttcaatt
     6481 caggtaattc ccgtaatggg aattattcac gttcattcag aacagagtat ggagaattaa
     6541 atttggtaat tcctagagat agaaatggta agttttctca acaaacttta cctgcttata
     6601 aaagaaccaa tgactcttta gaaaccacta ttattcagct atttcaaaaa ggaattacta
     6661 tgtctgaaat ctctgaattg attgaaaaaa tgtacgggca tcactatacg ccacaaacca
     6721 tttcaaatat gactaaaata gtttctgaag atattattgc ctttaaagaa aggtccttag
     6781 aatccagata ctctgtcatt tttatggatg ctactcacat tcctttaaaa agacaaactg
     6841 tttcaaaaga agcagtttat attgtaattg gcattcgatt agatggaacc aaagaggttc
     6901 ttgggtttag tcttgctcca actgaatctg cttatgtttg gaaagagatt cttcaagacc
     6961 taaaagaccg tggtttaaaa gaggttttat tagtcgtaac tgatggttta agtggcatta
     7021 acgatagtat tcatagtgtt tatccaaatg ctcaatttca acaatgttgt gtccatatat
     7081 ctagaaatat cgctcataag gttcgtgtta gtgatcgaca agaaatctgt agcgatttca
     7141 aattagttta tcaagcaagt tcaaaagaag aagcaaataa tcaaataagg tttatgatag
     7201 ataaatggaa aaagcagtat ccacgagtgg ttaaattgct catgaatcct gctatattaa
     7261 ccttttataa tttccctccg tcaatcagaa gaactattta ttcaactaat ttgattgaag
     7321 gttttaacaa acaattaaag aaatatacca aaagaaaaga gcaatttcct aatgaagaat
     7381 ctctggagag atttctagta tctcaattca acaactataa tcaaaagttt ctatgccgag
     7441 ttcataaagg ttttaaagaa atacatgata cattagaatc aatgttttaa cttgatcaac
     7501 agaatgggct tttccattta cacaaaatta ttgacgctcc cgacaaaatt atgatcttgc
     7561 gtaaagttat aaaatattaa taaaaatatt ttattaatga aaagcttggt agcacggtta
     7621 ttcatatgct aatttatttc actttatagt aaggggagtt cttagtatat tgttcaaact
     7681 taaatttcat ataatttaat tatgtaaatt agctattcta agaacttcta cggataactg
     7741 cccgtctaaa atttaaataa gagcttagaa tcttcatata agtaaggtta atttttttgt
     7801 acaaaaaagt cggaatattc tgagttactg aaatgatata aaatcataaa ctaaacccct
     7861 tgattttctt cacttttaac agagtatata tataagttca tgattatggt aaaatatata
     7921 tataatcatg aactcagaat atttttaaga agggaaacaa tagtgagcta taatgtacaa
     7981 ccgttaagga caaatgaaga gatacaagat tttcagtttt ggctaagaag aacaagtaat
     8041 ccagaaagag atagtttttt atttttgttt ggaatcaata atgggttacg tatgtcagat
     8101 attataggat taaaagttgg agacatacga ggtaaaagta agcctattat tgttgaacat
     8161 aaaacaggta agcgaaaacc gatttttata gataatttga gagaagagat tttattatat
     8221 actgaaggta aagaagaaaa tgattggtta tttccaagtc ggcaacaagg tcgacatata
     8281 acaagggatc gtgtctatca gatatatgca gatatagcag aaaaattagg aagagatgat
     8341 ataggaacac atacattaag aaaaactttt gggtatcact actacaagaa aactagagac
     8401 attgctacct taatgtttat ttttaatcat agctcgcaag caattacaaa aaggtacatt
     8461 ggtataactg aagatgaaat aggggcatcg ttacgtggtt ttaaattggg aggctaattg
     8521 agatattagg atatattttt tgtgtataat gaaaacttag aaggtgagat taatgaagat
     8581 aactgaattt tcggaagtat ttaaaacgtt tagtaacatt tacaatagaa taccagagat
     8641 agaacaaaat gttgtagttg ataatgatta ttatatagag aatgatttta ataaaccaag
     8701 aaaatatgac aacctataca attttataca caatagaaca acaataaata acaacagtaa
     8761 cactcgagtt ttgattaatt cagcatattt tcatattaaa tatttaaaga aatatgagta
     8821 tgaagaagtt gtattagatt atgggctaaa tagacagtca tttttaattt atgcaataaa
     8881 taacggaaat ttgccaagca ataaaaagaa cataattatt gaaacatatg cctatctgga
     8941 ggggggatct aaaaaactaa ttaagtcatg taatatagag tatcaattat cagtaggaga
     9001 aattaaatgt gttttcgaag aagatttaga acttgactta aaaaaaattt ttgccagcat
     9061 tcatgattat ataggtttga
//
LOCUS       DAYYAR010000111         8796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000111 DAYYAR010000000
VERSION     DAYYAR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.112"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACHAT0_002258"
     CDS             complement(<1..149)
                     /locus_tag="ACHAT0_002258"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8452172.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACHAT0_002259"
     CDS             complement(593..1714)
                     /locus_tag="ACHAT0_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFM8452173.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACHAT0_002260"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACHAT0_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM8452174.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACHAT0_002261"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACHAT0_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM8452175.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACHAT0_002262"
     CDS             complement(4304..5215)
                     /locus_tag="ACHAT0_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8452176.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACHAT0_002263"
     CDS             complement(5232..6239)
                     /locus_tag="ACHAT0_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM8452177.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSIPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACHAT0_002264"
     CDS             complement(6236..8365)
                     /locus_tag="ACHAT0_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8452178.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8495)
                     /locus_tag="ACHAT0_002265"
                     /pseudo
     CDS             complement(8370..8495)
                     /locus_tag="ACHAT0_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(8666..>8796)
                     /locus_tag="ACHAT0_002266"
     CDS             complement(8666..>8796)
                     /locus_tag="ACHAT0_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8452179.1"
                     /translation="RVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT         2688 a         1790 c         1548 g         2770 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggaattga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttttcagtca agtccacaat cttgtgtaaa
     8521 agttcaacct catcaaaagc acaatgtttt gcgttgtgaa cctaactgcc atgcctttct
     8581 ttctgaggag agaggtcagg agcagtagcg cagcgactct tgatgtctct cctcagaaag
     8641 aagagactcg cacaggttca tggtgctaaa tgttattcga attaaattct aaatattttc
     8701 gtggagaagc cagccactca gtgtcttttt ccatcaaaag tgttccaatt atccgagtga
     8761 tggaattgat atttggaaaa atccgaatca ctcgtt
//
LOCUS       DAYYAR010000112         8725 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000112 DAYYAR010000000
VERSION     DAYYAR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.113"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..904)
                     /locus_tag="ACHAT0_002267"
     CDS             complement(71..904)
                     /locus_tag="ACHAT0_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8452180.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1208..3373)
                     /locus_tag="ACHAT0_002268"
     CDS             complement(1208..3373)
                     /locus_tag="ACHAT0_002268"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8452181.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3633..5927
                     /locus_tag="ACHAT0_002269"
     CDS             3633..5927
                     /locus_tag="ACHAT0_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8452182.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5920..6585
                     /gene="pgmB"
                     /locus_tag="ACHAT0_002270"
     CDS             5920..6585
                     /gene="pgmB"
                     /locus_tag="ACHAT0_002270"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8452183.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6578..7585
                     /locus_tag="ACHAT0_002271"
     CDS             6578..7585
                     /locus_tag="ACHAT0_002271"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8452184.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7607..8626
                     /locus_tag="ACHAT0_002272"
     CDS             7607..8626
                     /locus_tag="ACHAT0_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8452185.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         2668 a         1893 c         1605 g         2559 t
ORIGIN      
        1 ctctccaaac ggtataatcg tttggagagt tttattttaa tcagtttttt tatgttatat
       61 gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga tcactgatga
      121 ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt ccttcaggga
      181 cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct attttctttt
      241 ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga agaagaaggc
      301 aactttctct tacagtataa taaccttgag agtctactgg attattgaaa tcacctagga
      361 aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac tcaaaataaa
      421 agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa atgtcctgat
      481 catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct ttcttttgaa
      541 attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt gcatttcctt
      601 cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac tcttttccgt
      661 gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata ggtacttgat
      721 tctttaccgg acagaacttc attagcttcg caggttctac ttcttcagat tccagtaatt
      781 ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta tctgctaatt
      841 cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt gtcatgaatt
      901 tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa atttttgtca
      961 caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct caatggacca
     1021 ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt ttcacgacct
     1081 ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca aacgaaaaat
     1141 aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa
     1201 cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta cctaattgct
     1261 tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt ggatcttttt
     1321 cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt cctacttctt
     1381 ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta tcgattccca
     1441 tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt ttgctaggga
     1501 aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt ggaataacag
     1561 caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca gataaaggaa
     1621 tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat tgcttactac
     1681 ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct cctgattgca
     1741 gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact gcttggactc
     1801 cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct tcagtcccta
     1861 ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg acatctttga
     1921 tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct gctgttcctg
     1981 ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg ttacgtcccg
     2041 gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta acaacaccaa
     2101 aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct ttgatagcta
     2161 acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat ggcaagatat
     2221 ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat ggcacagcag
     2281 caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca gccaacgctg
     2341 ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta cgatacatcg
     2401 caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact ttgaaacgag
     2461 ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca cctttaagat
     2521 tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact tgtgtccccg
     2581 cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg ataggaatcg
     2641 tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca aataaaaacg
     2701 gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa ttattgattc
     2761 cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt gaccataaga
     2821 taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc ggcaattttc
     2881 tataattata gtacttatta taagccattg ccccgacaaa tccagcgatg atccctacga
     2941 atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct ttaaccatga
     3001 tcttcgtacc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg tcacttgtga
     3061 caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca gctgcaaatg
     3121 ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat aaaatatgaa
     3181 gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg acaagcggcg
     3241 tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac ccagctgccg
     3301 gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaac tcaaaactaa
     3361 atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac gtattatagc
     3421 gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac cattctataa
     3481 aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt tttttaagct
     3541 tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc gtttattatg
     3601 aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga ctatttgcta
     3661 ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga ctgcaagaat
     3721 ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa gaaacttact
     3781 caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat aagacacgtg
     3841 tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac gccttgaact
     3901 ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct ttggaaccag
     3961 aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca ttcacaatca
     4021 ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt gtcaaaaaag
     4081 aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca gtcaaactcg
     4141 tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa catttttgga
     4201 atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa actattccaa
     4261 acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc gttaatggaa
     4321 aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc ttgtctgcag
     4381 aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt actagtagag
     4441 atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac cagacaaaca
     4501 attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg aagcttgccg
     4561 atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat ttgttccaac
     4621 tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa ggattcacag
     4681 gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc cctctttatc
     4741 ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat aatcaactgc
     4801 cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat cctatggtca
     4861 ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag attcaccgaa
     4921 atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat acggaatatt
     4981 tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca gaccgtgtcc
     5041 atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca aacgaatatg
     5101 agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc ttgcgttaca
     5161 cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc gaagaagaac
     5221 tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa gaattaggaa
     5281 tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc gatattccca
     5341 caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga tcttgcttca
     5401 tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt acaaaagaag
     5461 aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct tccctttcgc
     5521 caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct gtagaaatgt
     5581 accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa gacggcttgc
     5641 acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct caaatgaaaa
     5701 ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa gcttattctt
     5761 tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa caagttactt
     5821 tcgagttatt agaaggttct cctctagaaa tttacgtcta tgaacagtct tattctttag
     5881 cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg ggtattgttt
     5941 gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg gcgaaagctt
     6001 ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa aggcgtcagc
     6061 cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc tttttcaaag
     6121 gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat gatccagacg
     6181 atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt aaaagaagaa
     6241 aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact tgaaaaaatg
     6301 ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa cggaaaacct
     6361 gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga agaatgtatc
     6421 gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc tcaaccaatt
     6481 ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga tacgactttt
     6541 cttacgatcg attacttgaa agaaaagtgg catgaccatg gataatccag agatctccgt
     6601 cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact acttagaagt
     6661 cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca aagaaggaaa
     6721 atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag acaaaagctt
     6781 ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt ggggtgagca
     6841 tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag gctggtcttt
     6901 ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg tcttttactt
     6961 aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt accgtttaac
     7021 aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca tctgcaatcc
     7081 gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct atgaacatca
     7141 gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc ctactggaaa
     7201 ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac tgcaagagat
     7261 ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc ctaggctttc
     7321 caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta tagcaacttc
     7381 taataaaagt atggtattat tttctacgac tggattcgaa gctgatttct ctgtcaatgg
     7441 aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc ctgatatcgt
     7501 tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga tcagtcagac
     7561 gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg ctattaccgt
     7621 aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg tcatcaacga
     7681 tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg atgaactagg
     7741 ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg ccatcggcgt
     7801 aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc ttgagatcat
     7861 ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg ctactgctaa
     7921 aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac aagtagatgg
     7981 ttttattcta gtgtattcag atagtcaaga tactgtcatt gattatttat accaaaacca
     8041 catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg tttatgtaga
     8101 taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga atggacataa
     8161 gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac gatatttcgg
     8221 ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag tactagagca
     8281 tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca cagcattggt
     8341 ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt atggctatgt
     8401 cattccagag acactctccg tcattagctt caacaactcg atcttctcaa cattaacaca
     8461 cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg ctatgcaaaa
     8521 gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca tccctcatcg
     8581 cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag ctgactcact
     8641 aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat actacttcta
     8701 acacattgtt tatctgtaaa aaaga
//
LOCUS       DAYYAR010000113         8720 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000113 DAYYAR010000000
VERSION     DAYYAR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.114"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(149..562)
                     /locus_tag="ACHAT0_002273"
     CDS             complement(149..562)
                     /locus_tag="ACHAT0_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8452186.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            complement(638..934)
                     /locus_tag="ACHAT0_002274"
     CDS             complement(638..934)
                     /locus_tag="ACHAT0_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452187.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            complement(934..1140)
                     /locus_tag="ACHAT0_002275"
     CDS             complement(934..1140)
                     /locus_tag="ACHAT0_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452188.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            complement(1137..1334)
                     /locus_tag="ACHAT0_002276"
     CDS             complement(1137..1334)
                     /locus_tag="ACHAT0_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8452189.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            complement(1358..1663)
                     /locus_tag="ACHAT0_002277"
     CDS             complement(1358..1663)
                     /locus_tag="ACHAT0_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452190.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            complement(1660..1833)
                     /locus_tag="ACHAT0_002278"
     CDS             complement(1660..1833)
                     /locus_tag="ACHAT0_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452191.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            complement(1830..2399)
                     /locus_tag="ACHAT0_002279"
     CDS             complement(1830..2399)
                     /locus_tag="ACHAT0_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8452192.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            complement(2411..2545)
                     /locus_tag="ACHAT0_002280"
     CDS             complement(2411..2545)
                     /locus_tag="ACHAT0_002280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452193.1"
                     /translation="MKDWLEEASALVGCFINCAFISVIIALVGFVSLKVIILLWQLIF
                     "
     gene            complement(2545..2880)
                     /locus_tag="ACHAT0_002281"
     CDS             complement(2545..2880)
                     /locus_tag="ACHAT0_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452194.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            complement(2877..3263)
                     /locus_tag="ACHAT0_002282"
     CDS             complement(2877..3263)
                     /locus_tag="ACHAT0_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8452195.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            complement(3260..3577)
                     /locus_tag="ACHAT0_002283"
     CDS             complement(3260..3577)
                     /locus_tag="ACHAT0_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM8452196.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            complement(3577..3882)
                     /locus_tag="ACHAT0_002284"
     CDS             complement(3577..3882)
                     /locus_tag="ACHAT0_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452197.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            complement(3879..4040)
                     /locus_tag="ACHAT0_002285"
     CDS             complement(3879..4040)
                     /locus_tag="ACHAT0_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8452198.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(4055..4414)
                     /locus_tag="ACHAT0_002286"
     CDS             complement(4055..4414)
                     /locus_tag="ACHAT0_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8452199.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(4411..5262)
                     /locus_tag="ACHAT0_002287"
     CDS             complement(4411..5262)
                     /locus_tag="ACHAT0_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8452200.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(5279..6112)
                     /locus_tag="ACHAT0_002288"
     CDS             complement(5279..6112)
                     /locus_tag="ACHAT0_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM8452201.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(6119..6805)
                     /locus_tag="ACHAT0_002289"
     CDS             complement(6119..6805)
                     /locus_tag="ACHAT0_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8452202.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(6811..7557)
                     /locus_tag="ACHAT0_002290"
     CDS             complement(6811..7557)
                     /locus_tag="ACHAT0_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8452203.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            complement(7550..7891)
                     /locus_tag="ACHAT0_002291"
     CDS             complement(7550..7891)
                     /locus_tag="ACHAT0_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452204.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(7949..8077)
                     /locus_tag="ACHAT0_002292"
     CDS             complement(7949..8077)
                     /locus_tag="ACHAT0_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452205.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            complement(8070..>8720)
                     /locus_tag="ACHAT0_002293"
     CDS             complement(8070..>8720)
                     /locus_tag="ACHAT0_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFM8452206.1"
                     /translation="AESFDKKHRHVLTAIDELKEGVAENWADLFWEDTYVHPQNKQSY
                     RIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKELKDQLPSIPATKREMALLA
                     LAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMVRNKIRVICNQQHLNSKAKA
                     ELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMPSTATMTIIKQMELLEDE"
BASE COUNT         2211 a         1952 c         1322 g         3235 t
ORIGIN      
        1 ctatcgaagc ttaattgtga aacaataata aaacgatgtt ccttttatta ttattttgtc
       61 tcagacctat cactaatctt tcgacactac cataatatca ctggtaaata gctaaaaacc
      121 gccatcattc cgccaaaaaa ccgccaaatt atttataagc aattattctt ccgtgtttat
      181 atgcttctgc aaactctatt agagcttccg acttcatccg ttgtatactt ctttctgaat
      241 aacccacttc acggctaatt ctgtagtttg agaagctatc tggcacacaa aagctgtagt
      301 agagtatctg acggctaatc agactaagag ccatcaaagc cgctaaaatc gcatctctct
      361 ctgcttctat atccatcatc tgaatgatcg cgtcctctgc cttattgccg tgcttcggcg
      421 ccttcggcat atccgtaata attggagact taatatctat caaagagcga cctgccatcc
      481 gctccaaacg ccgaaagttc ttcagcacat ctctcgcatt acatcttgtc tgtttgaaat
      541 ctacctctcg taacaattgc atcaagtcaa accgctcctt tatgtgatat aataaacttg
      601 tctgttttat tatgttagtc ggagcgatcc ggctttttta tttgctctcg attacttcca
      661 tgtcaactaa tctcgctaca gctaaattct ctttgctttt cgctaaccac ttgtcacatt
      721 ccatcgtgtt ttcaatacga atgattgctg agttcgacaa taccttttct acatatccac
      781 gaaatggata gataaatcct tctgcttcac agcgaaccat gtcaccgact ttgaattttg
      841 gtttctcacg tgttttaggg ttctttgtcg gcatatctag catcaaaccg ccgataccat
      901 gactactagc gtaaaatccg tcttttagtt tcattactct accccctcat gatcgacagt
      961 gaccagttca tatacttgcg ttaatccacc aagtcgtcgt gaaacttggt tagcttcttc
     1021 gagattatca aaccatcttg cgctttggag tatgtctaca atggataatg tgttagcccc
     1081 tatagaacgt tcatcgcttc tgtaaaattg atttccaaac tttactacat aaatcttcat
     1141 tctgtatcct cccatttaat atctagtatc gaaattccaa acttacgaat agcatcactt
     1201 gcatcagcaa cacactgact tgccacttta tatgtttctt ctgcagaaac accatactcc
     1261 cgttcgaact ttactttcgc tacattcagt tcttgatttc ttagttttgc tactctgcgg
     1321 tgtctgttgt tcatttttct tcacctctgt tttgttatca ccatttcttt gttgaaaatc
     1381 cacaacgtct atactgttta attcgtttct taggcctttc tagatcaatc agaataaatc
     1441 tgtcggatat atgataatct tctattacag ccgtatacat tggattcgga accgagtacc
     1501 atgatctttt tttatcaaac tcatccgcat catctcttgt aatttctatc atgttagcag
     1561 acattagata gccctcttct tctgcctcgg atgggtggga atttgtatcc cagcttcgag
     1621 gaaacctggc caaatctctt tttaaataaa cttgaacatt cattccgctt cctcctcatc
     1681 gatctctatc tcgacatctc tgccttctaa aatctgtttg gtatcaatgc tttttgtttt
     1741 caacgccaac ccaccgtctt tttgtgcagc aacaatggta atcgtcattg cctttgttcc
     1801 gtctggtata tggagcatta tctgtctact catccttccg ccacctcttc cactggcaca
     1861 gcaaatggcc agtatctttc atcaattgct ttgatctgtt tttcggtcag ttggtagtgg
     1921 tgtgcaaggt attgtggcag gtaagctgtt gatactagtt taacttcatt actgcaactg
     1981 atctccttga caaggtactc gccagcaatt attacactgt acaacggctc tttctcgacc
     2041 tcgtagccgt tgtacaagct caatagtgtt tcatatgatt gtctcgttgc ccaagcgagt
     2101 aattcttctc cttgttcttg cgtcactatg ccatctcgta caaaccaatc acagaaataa
     2161 tatgaatcac catctttaga tttgattaaa tacgctattt tttcagcttt cgtaaaaggg
     2221 tccgtagatt tctcaagcca accggccacg agttgtggaa taactagctt cttcggttcg
     2281 tctatttttt tcattaacgt aatacctgca tttatactct cgttatagca agcagctagt
     2341 gcatcatttc ctatcacttt aatactttct aactttttga ttgcttcctg tttattcatc
     2401 gctgttcctc ctaaaaaatt aactgccaaa gcaatataat cactttcaat gatacaaatc
     2461 ccaccagtgc aataattaca gatataaacg cacagtttat gaaacagcca actaaggcgc
     2521 ttgcttcttc taaccagtct ttcattactg ttcctccttc aaccataaat ttggaaaatc
     2581 atcggcatat atctctattg cgccaaataa cttgatcgct gattttgatt ttccatgtat
     2641 gtcaggactg attaaacgat cataataatc ctgactgatt gtttttccct tgtactgata
     2701 acgtgtagcc gcagtgtaat atactgctgt cttgctcaga taaagatatt ttgctaaatc
     2761 agccgatatc cgtaacaaaa tgattctatt cttactttta attccacaat ttagcaatgc
     2821 tgggacattc ccattttcat cttttaaaga atcgatgtag tcagtagcta agataatcac
     2881 tctacctcca ataactcgct attctcgtgt atatttccgg tgacttcata ttcgtatccc
     2941 atcttttggt ggctgaatat tctatattcc attccactaa ttaatgattt acaaactaat
     3001 ccggaatgaa actcggattc ttgtacaacc gttttttcat taactaaatg atcaaaacca
     3061 ttacttacat taaccagaac tacatcccct tcaaatattt ctacgccatt cttatctttc
     3121 agtcctgtgg attgcataag cacatattta tctggagcca tttcagcatg agtgatcaac
     3181 cttcctgctt gcccatattt catttcttgt ccaattgttt tacctttaaa tggtgtgtac
     3241 cacgatcgaa atctcggtat catttgctgc cctccatgta ttcgtctaat atctctttat
     3301 atttctctac aaatttgaaa cgatcttgat gaagtttctt gctccaattt gtttgccgat
     3361 ccagctcacg catttgatcg aacccttttt gaatttcgtt gtaataaaat tcaatgtttg
     3421 ctgctgcctt ccaatgcctg ctacttcgca ctcctgcccc tgtttcagcc atttccaact
     3481 taactaattc cgctcgttct tttgattttt tgtctttctg aatcttcatc atgattttct
     3541 tgaggatgat atcactgtat tgtgtaatga gattcattat ttctcctcca catacctaaa
     3601 ctgtcttcct ttagaatcaa tatccatatt cttcgctcta tcccagatga tgtttttgct
     3661 cagaccagta atttcagata actgttcagc ggtacctgtt actagaattc ggtcaccgtg
     3721 ccagattgca atttttctcg gcattctccg tctggttttt tcagtccaca ttgatttacc
     3781 gagcttttgg acttctgcaa ctatttcttt gtcttcttgc caattctcag aatgtgtcag
     3841 ttcgatgatt cgtttcattg ctgctttctt atccacgctc attcctccaa tctacgaatt
     3901 tcccttctta agttctctat gtgcaaatcg attgcctttc tcgccgtttc attgaccatc
     3961 actgcctttg ttcgttccag atcgtcaatc tcacgttgaa tgcttcgaat tcgcatttga
     4021 atcacttctt ctgttgtcat gatagaccac ctctttaaaa acgctcttcc ttgaacgtat
     4081 tccgatattt tttagctaat atcaacggca cttgatattg atgacaaaac agctttgcct
     4141 tgatcttaaa gtcttttgtc tgcattcctt taacatctac gactttgaca agtttgccgt
     4201 ttttataaaa tgtgaagtcg ggaatatact cgatcttgcg atacttcttt ccgtctagtt
     4261 caaatttcgg catcagctca aatctttcct gaagttttac tttccagcca ttcgcttcag
     4321 cttgccacaa ggctagatcg taatactctg cttctgcgat agaatcaaac ttgatacctc
     4381 gatgaacagt ttttttatta cggtatttat tcatgcgata ctacctttca ctggttttat
     4441 gcgcttgtct gctgtttgct ggaatttcag cgcataacct tctgaattct taaatatcct
     4501 agaaacaatt ctttcaccgt aggcttctct tagttcagga ccagataagt ttgttgtgat
     4561 gatcgttgcc ttgttctgtc ttgcttctaa gagcgtgttt aacgtgttgt ttgtaaactg
     4621 cctactattt gataccccac tacctaattc agctccaata tcgtcaaaaa ccaccaaatc
     4681 agttgtttta atatcggcta taagcgatcc ttcaatttct tttctcagtt cagcattgtt
     4741 ataagaaaac tttatttgct ctaataactc ttgatagctt ataaaaagta ttttcttgtc
     4801 ataatttgag cgctcaagta tttcccaagc tgtcgccatt gacaagtggc tttttccgct
     4861 tcctgatttc cctgatagaa tgaaatgtgc aggatggttc agtaagacat catttacaaa
     4921 gcttttagct ctttctaaag caattttcgt ttcttggtcc actacgtgat aattctccat
     4981 tttgcattta aacaaagttt tatctgttaa taccgaacca ttttgaaaaa aactcaacgc
     5041 tcgtgctttt aagctgtcgt tatatatccg ttcggtctgt atatcctctt tcacacgtaa
     5101 cgctttataa ccacaactca tgcatgttgg tttacaacgt tctgaaccat ccttattttt
     5161 agctcgccaa ctatacaaag gttcgctaca ttctggacat ttcccgcttt gcactaatac
     5221 tcttcttatt agtttctcca tagcatttgc taggctttcc atgtgatgca tctccttttt
     5281 aaattggcaa gtcgtcatat tcactaggat tgctgtactg tagtttttga ctttgctttt
     5341 tatgattatt cttgtctgct ttgatttcga atttgagctt ctcaaatttt tctctcaatt
     5401 tcttagcact tctaatattt ccaaaccaaa attcatttgt aggtagccaa ttgatcacat
     5461 actcaatcgc ttctatagac gctttatctc tttcttccat caacctgatt gtgtctgccc
     5521 atttttcgat atctactttg ttcatttctt ttggaaaatc ttcagttaaa ttactttgca
     5581 attttttagc aaggcgtaag tgttcgtcag aatacttacc tttcttttct tctttatcta
     5641 tatctatatc tttctctatc tctatctcta actctggtgt agttttgtct ggacatttgt
     5701 ccgacacttg tcctccagtt attaaattcc gttttgcctc ttctattttc tttctgtatt
     5761 ctctttttct atctgcttca gttgaggatt ttccaatgaa actttgtata tcagacatat
     5821 aaattgctcc gttatctaat acgtcaataa gctgcaaatc acggaaaatt tgtaccgctt
     5881 tttctacgac tcctacagaa tgtcttgtaa tagttgcgag cattgtagag ttaaatggaa
     5941 tcctgtcatt aaacatcaac ttaccttcgt gttttagact tcttaaataa agtttgagaa
     6001 gaatattaga ataaatatag ccatctggca tactttctaa gagaaccatc tcgtcactat
     6061 cgaaaaaatt ctcttttagt tttaaatagt agtagcgttt gttgtcagac aatatttttt
     6121 accctcctat tctaagtttc ttaattgttt cctggtttaa cttgatccct ttgatttgat
     6181 atttattttt gaaattgatc acacctatat tgtgtttctc cgtgtgatga atcctgcaga
     6241 gtgctgcaaa tgtgtactct gaatgatcaa cttctttgcg ctttcgtctt cctagcgctt
     6301 tgtcaaagtg atcgatgtca gctcctgttt tgccacagat gcagcagact ctttttgtga
     6361 tgcatttgta gaagtaatat tcttgattcg ctggtaaaat ctcatagcct tctttgaaag
     6421 gaatatgatg ttcaaagatg aaatctaaga tgatatttgc taagacatta gcatcactca
     6481 cagttgtatt cgattcgtct ttgaggctta ttttgcgccc tgtgacgcct tcaaaacgga
     6541 agtaaaagaa ttccttccag aagtccgttg gcatgcctgt atcgatgaaa atatcgccta
     6601 tcagcgcata gatgaagttt cgttgctgca cagtaaatcg acgtggatca ataaatcgaa
     6661 cttcaataat ccgatcgcca tcatatccgt catacatcgt ctttagtcga tcaatgttca
     6721 cttcttcatt gatcgttgca cctatgtctt ttcctttgaa ctttttcaga accgctgaat
     6781 atgaatcgat caatggttta aacactcata tcacttctct tttgtttctt ctctgtactg
     6841 atcttcaatc cagttaacgc ctcgttttag aatgcccaag tctctcttgg tccatttact
     6901 gtcatcagcg gttatagaag ccgcatcagt caatgcaaca attgcttcat caattgattt
     6961 ttcgtacttg ttagcaacca gttgtaaagc atccaagaat agctttttgc ttctttgagt
     7021 agctggttca agcatcgaga catcttctgg catatcttcg ccagcaaata tatatagccc
     7081 tagcccaaac attgctagat tttttacaag acagcgcatg atcgttttgt tgatatcaaa
     7141 catagttgct gcttcaactc gcttttcgat ttttccaaca atctcttttt tcttcgtttc
     7201 gttattccac tgataatcat tgacttcgta ggtatatggc tcatctttca ttgccttgtt
     7261 tgcaccatcc atgactggta accacatgtc acgctttact ccgttgactg tgatactggt
     7321 aaaaaccata tagcctgttt tcttatcaaa gacatacggc agatatgttt ctggatcatg
     7381 atagatttca taatctactg cctcacacac tttgctaact tctgcccaag cccatgccca
     7441 cgaaagataa gtgaggttat ttcgtttttc agtaacatct ttcacattta ttttgtataa
     7501 ggtattaaaa agcgtattat cacttctatt tttcaaaggt tgttcctttt cactcatcaa
     7561 attctgcctc catttcagca atgtatttct tacctgatcc gtaataagag atatcaatca
     7621 agttatctct gtcatactct tctagcgcat caatcaagcc atcttcgatg acatagatat
     7681 attcagtttt tttggacttc ctcgataaat ggataagata gacatgatcc caaatactca
     7741 caaaatttcc caaatcgtct tgatcacatg ctagttcttc atccgtcaaa agattacgtc
     7801 tgatttttcg attgcttgtt tccttgatat tcgatttgcc ccaactagga tcagtcaaat
     7861 attgatctag agtggaaagt tcttttttca tgtggtaaca tctccttaga tgtattttct
     7921 ttgtgactca ttgctttggt cggctgagtc acttttttat ttgttgccat gctttttgct
     7981 tatcaatatg ttgttggctt aggatgtttg gtttattgtg tctccaccag cgattagcaa
     8041 ttattacgcc tatttttagc gcttcagctc tattcatcct ctaacaactc catttgctta
     8101 ataattgtca tcgtcgctgt agaaggcatc caattgttaa taaaatcgat aacatcatcg
     8161 aactgttttg ccttgatacg atttctagct actgctccag tgattcgctt aattccgcca
     8221 ttcaagtctt taaacaattc tgcttttgct ttgctattta aatgctgctg attacaaata
     8281 actctaattt tgttgcgaac catattagaa attgttcggt aatctggttc agctagaagt
     8341 tgattgttct tcaaatcgtt taaatcagac tcgatagcgt ctactctttc attcgtttct
     8401 tcgtttgcag ctagagcaag taatgccatt tctcgtttag ttgctggaat tgatggcaat
     8461 tggtctttta attctttttc catcttgttg aaggcctcaa tatattgaag tttaaaactt
     8521 aaagctttct taccagtgaa gcccattgct aatagagaga agccgtctct attcatataa
     8581 ataattcggt atgattgttt attttgagga tgaacataag tatcttccca aaataggtct
     8641 gcccaatttt cggcaacccc ctcttttagt tcatcaatag ccgtcaaaac atgtctgtgc
     8701 tttttatcga agctttctgc
//
LOCUS       DAYYAR010000114         8675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000114 DAYYAR010000000
VERSION     DAYYAR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.115"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACHAT0_002294"
     CDS             <1..1101
                     /locus_tag="ACHAT0_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8452207.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACHAT0_002295"
     CDS             complement(1280..1615)
                     /locus_tag="ACHAT0_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8452208.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACHAT0_002296"
     CDS             complement(1599..1862)
                     /locus_tag="ACHAT0_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8452209.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACHAT0_002297"
     CDS             complement(2322..3125)
                     /locus_tag="ACHAT0_002297"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8452210.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACHAT0_002298"
     CDS             complement(3103..4227)
                     /locus_tag="ACHAT0_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8452211.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACHAT0_002299"
     CDS             complement(4243..5058)
                     /locus_tag="ACHAT0_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8452212.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACHAT0_002300"
     CDS             complement(5048..5920)
                     /locus_tag="ACHAT0_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8452213.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACHAT0_002301"
     CDS             complement(6005..7294)
                     /locus_tag="ACHAT0_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8452214.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7971)
                     /locus_tag="ACHAT0_002302"
     CDS             complement(7438..7971)
                     /locus_tag="ACHAT0_002302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452215.1"
                     /translation="MANEKATMTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQL
                     SWAAIWGKHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAR
                     EVGQQLGLTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGV
                     KRFGDGIHDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACHAT0_002303"
     CDS             complement(8017..8160)
                     /locus_tag="ACHAT0_002303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8452216.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DAYYAR010000115         8312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000115 DAYYAR010000000
VERSION     DAYYAR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.116"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHAT0_002304"
     CDS             complement(105..629)
                     /locus_tag="ACHAT0_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8452217.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHAT0_002305"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHAT0_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8452218.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHAT0_002306"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHAT0_002306"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8452219.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHAT0_002307"
     CDS             complement(2351..3031)
                     /locus_tag="ACHAT0_002307"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8452220.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHAT0_002308"
     CDS             3157..4026
                     /locus_tag="ACHAT0_002308"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8452221.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHAT0_002309"
     CDS             4052..4567
                     /locus_tag="ACHAT0_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8452222.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHAT0_002310"
     CDS             complement(4614..5177)
                     /locus_tag="ACHAT0_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8452223.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHAT0_002311"
     CDS             complement(5232..5411)
                     /locus_tag="ACHAT0_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452224.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHAT0_002312"
     CDS             complement(5488..5997)
                     /locus_tag="ACHAT0_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452225.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHAT0_002313"
     CDS             complement(6093..6473)
                     /locus_tag="ACHAT0_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8452226.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHAT0_002314"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHAT0_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8452227.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..>8312)
                     /locus_tag="ACHAT0_002315"
     CDS             complement(7873..>8312)
                     /locus_tag="ACHAT0_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8452228.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         2374 a         1714 c         1500 g         2724 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt ga
//
LOCUS       DAYYAR010000116         8249 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000116 DAYYAR010000000
VERSION     DAYYAR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.117"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACHAT0_002316"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACHAT0_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8452229.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACHAT0_002317"
     CDS             complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACHAT0_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8452230.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACHAT0_002318"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACHAT0_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8452231.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="ACHAT0_002319"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="ACHAT0_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8452232.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACHAT0_002320"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACHAT0_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8452233.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="ACHAT0_002321"
     CDS             complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="ACHAT0_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8452234.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8134..>8249)
                     /locus_tag="ACHAT0_002322"
     CDS             complement(8134..>8249)
                     /locus_tag="ACHAT0_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFM8452235.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
BASE COUNT         2200 a         1816 c         1440 g         2793 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc aaaaaaactt gcggcagctg tcagtagctg ctgcaagttt tcgttgttat
     7441 ttaagtaagg ctgatcagtc tgtttcaatc agtccgtatt ttccatcttt acggcggtaa
     7501 acgatacttg taccatttgt ttctgcatct tcaaaaataa agaagttatg tcccagcatg
     7561 ttcatttgta agactgcttc ttcactatcc attggtttca atgaaaggcg tttcgtacgt
     7621 acgatatcta attcagaatc attttcgttt tcgtcctctt cattgaataa agcagcagca
     7681 tctgcagtat tcatgcccgt ttcacgggat ttacgattaa tttttgtttt gaatttgcgg
     7741 atttggcgtt ccaatttgtc aacaaccaaa tcgatacttg cgtataaatc tggagaagtt
     7801 tcttcggcac gtaatacaag ataaggtaat gggatcgtta cttccacttt tgctgttttt
     7861 tctgtataaa cttttaagtt cacatgcgcc gttgcatcgg gtgcgtcact gaaatagcgt
     7921 tctagtttgc ctactttttt ctcaacgtag tcgcgaatcg cttcagttac ttcgatattt
     7981 tcgccgcgta cattatatct aaacataaaa attacccctt tcgtctacca caatcaagaa
     8041 ggagaaggac cactctttcc ctcttcttaa ttctattata tcgaactcta ggttatttgc
     8101 aaattaaatc gctatcaaat ttccacatct gattcatctt gccaaagaaa tggttcggat
     8161 gattttcggc tgtgcgttct gcaagcaatc tgctgcatga aaaagcgtct gtcctgttgt
     8221 atagacatcg tcaataatca atatttttt
//
LOCUS       DAYYAR010000117         8223 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000117 DAYYAR010000000
VERSION     DAYYAR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.118"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..377
                     /locus_tag="ACHAT0_002323"
     CDS             <1..377
                     /locus_tag="ACHAT0_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8452236.1"
                     /translation="NNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQD
                     RELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKN
                     PNMFVNYQNDPYQFTTQVILLF"
     gene            392..1144
                     /locus_tag="ACHAT0_002324"
     CDS             392..1144
                     /locus_tag="ACHAT0_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8452237.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1847..2062)
                     /locus_tag="ACHAT0_002325"
     CDS             complement(1847..2062)
                     /locus_tag="ACHAT0_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFM8452238.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2081..2317)
                     /locus_tag="ACHAT0_002326"
     CDS             complement(2081..2317)
                     /locus_tag="ACHAT0_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFM8452239.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2575..4728
                     /locus_tag="ACHAT0_002327"
     CDS             2575..4728
                     /locus_tag="ACHAT0_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFM8452240.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4697..6112
                     /locus_tag="ACHAT0_002328"
     CDS             4697..6112
                     /locus_tag="ACHAT0_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFM8452241.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6145..7179
                     /gene="sppA"
                     /locus_tag="ACHAT0_002329"
     CDS             6145..7179
                     /gene="sppA"
                     /locus_tag="ACHAT0_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8452242.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7181..7702
                     /locus_tag="ACHAT0_002330"
     CDS             7181..7702
                     /locus_tag="ACHAT0_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8452243.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7699..8085
                     /locus_tag="ACHAT0_002331"
     CDS             7699..8085
                     /locus_tag="ACHAT0_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8452244.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2925 a         1186 c         1478 g         2634 t
ORIGIN      
        1 ataacaataa gatttgttgt aaattcgaat gtccaagtac tattattgaa gttcctgttc
       61 caatatttga ctgtattagg gtgcttggaa ttattctcga taatgcaatt gaagcatcta
      121 tagagagtca agatagagaa ctgtctctag ctatttaccg agatgataaa cagatagaaa
      181 tttcgatatc gaatagttgt agagagatac tactctctat agatactcta atgaaaaaag
      241 gaataacaac aaaaaaggga catcaagggt taggtttatc tagtatagag gaaatcaata
      301 aaaagaatcc caatatgttt gtgaactatc aaaacgatcc gtatcaattt actactcaag
      361 ttattttgtt gttttgaggg aaggaggatt tatgagttat ccaataatat tatgtgaaga
      421 tcaaattata caattaaatc agttagaacg aattattgat aatttcattc tttttcacga
      481 taaagtattt aagatagtat tgaaaacaca aagtccgtta gaagttaaaa aatacctcaa
      541 acaattccga ccaaagcaag gaatatattt cttagatatt gatttaaatc atgaagttaa
      601 cggtatagaa ttagcagaag taatcagaaa atatgatgtt caagcaaaaa tcatttttac
      661 aactactcat gatgagatgt tacccgtaac aataaaaaga agagttgaaa cgttaggatt
      721 tgtaacaaaa gatcaaacac tagatgagta tcgaaacgag attgttgagt tattgttatt
      781 agcgcaagaa aggatagatg caacaaaaca ggttacgaat caagcatttg ttttttccat
      841 aggttctcaa acatttactt ttgatataaa tgacatttat tttgtagaag cttcaaaatt
      901 accacataga ttatcattgt gtactaaaga tgggcagtac gaattttatg gaagaattag
      961 tgagcttgaa aagaaatatc ctatgttaac aagaattagt cgagcgtgtt tagtaaatat
     1021 ctttaacgtt aaagaaatag attttaaaaa acgttcacta tattttgatt cagagttggc
     1081 cagaaacttt actttaggga aagcacaaaa aattaaggag aagttgaaag aaagcactgt
     1141 ttagtggttt tttctttagg gagattgcat ttttctaata aaatagattc cttcactagt
     1201 gcaaattttt ctgaatccta ttaaagttgt accaattttt atagctgtta ttagttacgt
     1261 gcataacagc aatattagtt actggaggaa tatttctatg gtctggaacc aaaaaagggc
     1321 caaatgaata gttcttttct tgtttttatt aagtaccata aaggggaatt aaaattaaat
     1381 gtatggtgga agatgcgaaa gatttggcaa attctactaa tttttagaaa gtgtttttca
     1441 ttttttagtt aaaaaatttg tcctgtcaag tgcaatccta tgattgtact tgataggatt
     1501 tgagataatt actttcctgt ttttataaat ttaacttgca ggaacaggct ttaatgcaca
     1561 tccgacaagt acattaccaa tacctcctgt aacggccgaa ataggtcctc ctgcagcacc
     1621 aactacagcg ctgcaagtcc ttttgtaaaa atatctctac cattacattt ccaaagaatt
     1681 tttatctgac tttaataaaa gataaaaatt taatataatt ataaaaacta gcacaagaag
     1741 ctttagtaaa aagttctgtc cattccaaac ttttgaaaag ataaagaaca tacctatgtt
     1801 cgtaatcgac acaaaccaat cttgaggttt gatatttttt aattgtttac gcaccggtcc
     1861 acatgctgaa tccagcacct aataaagctc cacctacacc acaagcagct attgcataag
     1921 gtcctccggc tgaacaaagt tttactcctg ctgttgcccc agaaaaagta gtgcctaaaa
     1981 ctcccacggc actcccacct tgaatatttt tcaactcttc tgaagataat ataaatgttg
     2041 tttttgtgtt tttaatatgc atactactta ttctcctttt ttattttttt cgagcatccc
     2101 agccagctgc agcacctcca gcgacagctc catataaccc tccaacaaca ccgcaagctg
     2161 gtccgcaaat tgctgttcct aaacctgcac cacctactgc ccctgtggta agtccaccta
     2221 caacttgccc caattttccc caagcattat tgcctccacc aacagtttgt gcatttccac
     2281 aatacttagt tcctttacat tttgcatgtt taaacacgct tctttaaata atatttttta
     2341 cggtgctact gtaacatacg tatttgatta acagtaaaaa tcttcctgaa acttatcaag
     2401 tttattcttg aatagtaaaa tttccaaaat aaatttgatt caggaatgaa aaagttagtg
     2461 ttcaagaact gttttcaaag tttttgtgga attatgagac aatattttca ctgtattaaa
     2521 gctatattga gaatttttct ataaaaattt tttagaagga gttgtgttag ataagtgata
     2581 aaatcatttc cttttgtgca gcaacaagat gaaaaagatt gtggtgttgc ttgcctttcg
     2641 atgattttaa aatattttaa aactgatatc ccgattcata aattacggga cctatctggg
     2701 actgatttag agggtacttc agcatttgga ttgaaaaaga catttgaaaa attgtctttt
     2761 gattgtatgg ctattcaagc agatagtgag atctggaaag aaagagatct tatcttacca
     2821 ttaattgcac atatattggt tgataacagc tatatgcact acgttgtaat ttataaaacg
     2881 aagggaaacg tactatggat tgcagatccc gctaatggaa aaaatagaaa aacgattgaa
     2941 gcgtttcaaa aagaatggac aggtattctt ttattaccta tgcctagaga aacttatatt
     3001 cctattaaag aaaaagtagc tggattaagt tcttttttgc ctataatttg gaaacaaaaa
     3061 ggattagtat tccacatcgt tttagcatcg attttcatta cgttttttgg cataggaagc
     3121 tcatattatt ttcaaggtat tcttgatttt ttcatcccta atcaagcacg ttcaacttta
     3181 aatataattt ccattggctt gatcgctgtc tatttatttc gggtattgtt tgaatatagt
     3241 cgtagttatc ttcttattat tttaggacag cgaatgagta tggcaattat gcttcagtat
     3301 ttcaaacatg tgttaacttt accaatgaat ttttttgcta cgagaaaatc aggggaaatt
     3361 atttctcgtt ttcttgatgc cagtaagatt attgatgctt tagcaagtgc tacgttatca
     3421 atatttttag atattggtat ggtgttgctt gttggggtta cgttagctat tcagaatagt
     3481 acgttgtttt taatcactct atgctctttg cctttttatg tagttgcaat attcgctttc
     3541 gtgaaaagct atgaaaaatc gaacagggaa gaaatggcag ccggtgcagc tgtaaattct
     3601 agtatcatag aaagtcttaa aggaatagaa acaattaaag catatagcgg tgaagagaaa
     3661 gtatataacc gtatagacca tgaatttgtt aaattaatga aaaaatcatt tcgtacggtt
     3721 acgcttgata atattcaaca aggaattaaa catttgattc aattaattag cagtgcatta
     3781 attttatggc taggttctta ttttgttatg gataatagaa ttagtctagg tcaacttatc
     3841 acatacaatg ctttactagt atttttgaca gatcctttac aaaacattat taatttacaa
     3901 gtgaaaatgc aaacggctca agttgcaaat aatcgtttaa atgaaatttt cgcgattgat
     3961 tcagaaaaat cgaaccagtc ttttgaatat atgctttctg aacaattatt tcaacaagat
     4021 atcactgtaa aaaacgtttc attctcgtat agcatgaagg ccccaacgtt gaacgaaatt
     4081 tcatgtacaa ttccttcgca cagtaaaacg gctatagttg gagttagtgg ttctggaaaa
     4141 tcaactcttg caaagttatt tgttcgtttt tatgaagcat cagaaggatc gatccattat
     4201 ggaaacatta atcacttaga cattcctttt cagatattac gcgatcgagt gacatatttg
     4261 cctcaagagt catttttctt tagtggaaca atcattgaga atcttatatt cggtttagat
     4321 agcccaccaa gtttcgaaca aataatagaa gtttgtgaga tagtccaact aaatgaattt
     4381 attaatcaac agccattacg ttacgagaca ttgttggaag aaggtggaaa taatctttca
     4441 ggagggcaac gacaacgatt agccttagca cgggctttat taaaaaatcc ggacatatta
     4501 attttagatg aagcaactag tggattagat accttgttag aacatgcaat tatgaattat
     4561 ctcctcaatt taaaagaaaa aacgattatt tttattgcgc atcatttacc aatagcaaaa
     4621 gcatgtgatc aaattttagt tctccatgaa gggcatttgg tagaaaaagg aacgcacgat
     4681 gaacttcgtt ttgccgatgg tatctatcaa aaattatggg aaatctagga gagggaaaca
     4741 atttatgcag aataataatt ggttcgaaca ttctagtatc tatactaaac aacatcatag
     4801 cttctaccgt tgggtgttat accctgtaat tcttttttta tgtattattg gattgttttt
     4861 aacctttgct aaaaaagagg ttgttattcg gacaacggct caattgaacc ccgaaaaaat
     4921 agagaagtta caagtgccat tagaagctaa aataatggaa aacttcctaa aagaaaatca
     4981 atttgttcat aaggacgatg tgttggttag attggattgt tctttaatag agaatgaaaa
     5041 ggctcagata gaacaagaaa accaacgtat tactcaacaa ataaagatgg ctcagctatt
     5101 tattgaaagt ataagtaaag gaaaaaattt gttttcaacg gatgacagtt ttggctacag
     5161 taatcaatta aagagcatgt tgtcagaaaa agaatcactc cgctacgctt tgaagcaaag
     5221 tgaattaaat gatcaaaagc aattagaagt atacgaaaag acaaaaagac aactagaaaa
     5281 acaaattgag agttcagata gtaaattaca agaatggcaa caagtacagg tagcttggag
     5341 taataatcaa tcattaaaag atttttcaaa agaaatgatg gcaaactatg agaattggca
     5401 agaacaacta aataatgttt ctgatgatca aaaaaatcaa gtgaaactga caatttcagc
     5461 aagcataaat gaacaaattg agcaactaaa aaaagaagta gaacagtatc agtcagaaaa
     5521 agctaaatta gttaaaccaa ctacttctga gaatgacaga attagtcaaa cggaaaaagg
     5581 aaagcaagag ctagaacaag caataacaac tgtaaaacaa acagttacag agctaaatga
     5641 aaaacaagag aagaaccagt cagttatcaa aagtttggac gatcaacttt caaaaggaat
     5701 actaaaagct cctgtatctg gaacggttca tttaaatgaa gaaacaaagg gacaagcaga
     5761 actagccaaa ggaacagtgc ttgcagaaat ttatccgatc cacgaaaaat caaaaatgaa
     5821 atttacagca cttttacctg cgaatgaaag tacctatatt aaagaaggga tgaaggttca
     5881 ttttaaattg gatcaaaaag gtggggctcc gattacgatc gacggaactt tagatgaaat
     5941 ttcagaaaat tctactgcca ctgagaaagg tgttttttat gcagtgaaag gaacgttaaa
     6001 aacaactcaa tcgtttccgt ttcgttatgg attaactgga gagttgtctt taattgtagg
     6061 agaaaaaaca tattggcaac aaattgtcga tgtgtttcta aataaaaaat aattattttt
     6121 actaagaata taggaggagt ttatatgaat aaaagaagat ggattgctgt gggagtagcg
     6181 tgcggattat tgttactatc cattatcgtc gcgctaattc caggaaaaga taaagaggaa
     6241 gcatcaaata caaccctaac aggaataaat aagatttttt acggatctaa cgaaatcaca
     6301 gaagaaacgt tagaagaagg cgcatcaaat aaaaagattg tcaaactatc cgtcaatgga
     6361 gttattgcag atacaggtga aagcaatctt tttagtaggg aacaatataa ccatcagaat
     6421 tttttaacac agctaaagaa aattcaagaa gacaaagcgg ttaagggagt gttattagaa
     6481 gtaaattcac ctgggggagg aatatatgaa agtgctgaga ttgctaaaga aatggcaaaa
     6541 attaaaaagc tagatattcc tatctatact gcatttaaaa atacagcggc aagtggaggg
     6601 tactacattt ctgctggttc agataaaata tttgctacgg aagaaacaac aactggatca
     6661 attggtgtca ttatttctgg tttaaattat tctggcttat tagaaaaact tggcgtaact
     6721 gatgcgacgt ataaaagtgg tgcattaaaa gatatgatgt cacctcaaca caaaccttca
     6781 gaggaagaga ataaagttat acaggaattt gtgatgagtg cttatgatcg ttttgtcaat
     6841 gttgtagcaa aaggaagaaa tatggatact aatgcagtga aagaacttgc ggatggaaga
     6901 atttatgatg gtaaccaagc agtagaaaat gggttggtgg atcaaattgg ttactctgaa
     6961 gatgcattag attccctaaa aaaagagaag aaattaactg atgcgacggt tatagaatat
     7021 aaaaatgaca caactggctt tgcttcttct tggttaggta ataagattgc tgaatggcaa
     7081 gggctaaagg caactacatc agatcgtgta atttcagttt ttgaaaaact tggattttct
     7141 gatactccaa aaccaatgta ttactatgga gggctataaa atgaataaga agagtggcat
     7201 tgaaactttg cctaattatg tatttgcggg attttggtta cgattttttg cttttcttat
     7261 tgatttaatt tgtattagca tcatcaccca tgctactata ggtctctttt acaaaatcat
     7321 gggactggaa tctgacaaat ccattattac tttttatggt atagcgtcat tagctattta
     7381 tttaacctat tttgtacttt tgactaagct gaattcagga caaacaattg ggaaaatgat
     7441 ttttgggata agagtagtga gctttgagga aaaagagcta agttggcaaa ctatctttat
     7501 tcgtgagtgt atatgtaggt ttatattaaa aaattttgta tttcaaattg ggtatttggt
     7561 ggctgctttc acgcccaaga aacaacatat tggggattat ttttctaaca cgtcagtggt
     7621 tgttattcgt tcattagaag ctattaacta tggtcaaaaa atttctaatt ctaaaacact
     7681 taatctggag agaattaaat gaataagaga aaaacagagt ttattttgat tttaattagt
     7741 ggaataacag ggattttatg tagtctttta gcattgatgg gtggtatgtt tctatcttct
     7801 attgattcaa ctactttaaa tactacagaa ctaaccagta aggatataga tatgttagtc
     7861 aatctaagcg ggaatgttgt tttatttgga ggaattaccc tagttttttc gataatgatt
     7921 ttcataatag gttttttagt aagaaaaaat caaaaaacag tggtctatgg tattagttta
     7981 ctcattttat ctattattcc attttttctt ttaacattat tgtgggtaat tcctggagtt
     8041 ttaggtatta ttgcgggatt gatgcttcta ataagaaaat cttaattagg caatgtgtag
     8101 acaacgtgaa acgattaatc aattaaaagc taaatacgtt ttgagaaatg ataacttgta
     8161 gtcgatggaa gacatgtaga taaaaaaact acgtgtcttt tatttattct aattttttcg
     8221 aaa
//
LOCUS       DAYYAR010000118         8163 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000118 DAYYAR010000000
VERSION     DAYYAR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.120"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            473..1336
                     /locus_tag="ACHAT0_002332"
     CDS             473..1336
                     /locus_tag="ACHAT0_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8452245.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1465..3087)
                     /locus_tag="ACHAT0_002333"
     CDS             complement(1465..3087)
                     /locus_tag="ACHAT0_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8452246.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3336..3857
                     /locus_tag="ACHAT0_002334"
     CDS             3336..3857
                     /locus_tag="ACHAT0_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8452247.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4043..5773)
                     /gene="spxB"
                     /locus_tag="ACHAT0_002335"
     CDS             complement(4043..5773)
                     /gene="spxB"
                     /locus_tag="ACHAT0_002335"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8452248.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5938..6756
                     /locus_tag="ACHAT0_002336"
     CDS             5938..6756
                     /locus_tag="ACHAT0_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8452249.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6859..7056)
                     /locus_tag="ACHAT0_002337"
     CDS             complement(6859..7056)
                     /locus_tag="ACHAT0_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452250.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7225..7803
                     /locus_tag="ACHAT0_002338"
     CDS             7225..7803
                     /locus_tag="ACHAT0_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8452251.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7898..8035
                     /locus_tag="ACHAT0_002339"
     CDS             7898..8035
                     /locus_tag="ACHAT0_002339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452252.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2444 a         1473 c         1610 g         2636 t
ORIGIN      
        1 ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
       61 aagctgtttt ttgaaattgt tccttgtgac tcggagctaa acggcaggct cacaacctct
      121 tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga
      181 ggaactattt tttccgtttt tttctctaat actcaggtca aaacgctgat ttcacaacct
      241 ctgattcacg gtgttccaga agtagcttct acggaaataa gccgaaattt cacaaaaatt
      301 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac
      361 ctcttatttt gattagaact tgcgaaaata aaccggttaa agtgtgtcta gcgcaagaga
      421 tttgctataa tagacgataa gggtaaaatg taacgagaag gggttgaaaa atgtgtatca
      481 aattgtaaca gattcttgtt gtgatctgcc ataccatgta ttagcaaaag aaaaagttga
      541 ttttttatca atgaatctcc aattgaatga tcaagagtta gtagatgacc tcggaaagac
      601 attcaattat gaccgctaca tgaatcagct gaaaaatggt gcgatgccta cgacatcaca
      661 aatcaatgtt ggccgatatt tagaattttt ccgttcttat gcggaaaaac agattcctgt
      721 tttatatgtg gctttttcca gtggattaag cggctcttac agtagtgcgc ttcaagcggt
      781 tgaattgctg aaggaagaat atgaagaccc tgagatctat gtttttgata caaaagctgc
      841 aagtattggg gaagggcttt tagtattgga agctgtaaga ttgaaaaaag aaggtaaaag
      901 catcgaagaa accctagagt ggctggaaag taattatttg aaagtccatt catgggtaac
      961 agtagacgat ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa gtgctactct
     1021 tggcagtcta ttgagtgtaa agcctattat tacggtggat aaagaaggaa ggctgacgaa
     1081 tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg agacggtaaa
     1141 aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct tacgctgggg atctagagag
     1201 cgcggagaag gcaaaacggc ttttggaaga gcagataaag atgaaagatg tgaagcttta
     1261 tcccttagga ccaacgatcg ccagtcacac aggctatggc tgtatcgcga ttttttcgat
     1321 gggaatcagt cgataaaagc agtaactcat tctgtaaaaa agcccagaaa ttgattgtaa
     1381 caaaaacagc gactcttaaa agatagatga gcatcctatc tcttagaggt cgctgttttt
     1441 ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt tttgaatatc
     1501 tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac ctttatccga
     1561 aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg acgcaaataa
     1621 gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt ctaagtctaa
     1681 atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca tttttgaaag
     1741 catgcaacgg actttctctc caccagataa aacattcact ggtttcaata cttcttcacc
     1801 agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt cttctttact
     1861 tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt tcgttgtatc
     1921 ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag tatctggcgt
     1981 aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt ctgcgacgaa
     2041 agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt tctttccatc
     2101 aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac gttcaggttt
     2161 gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag taattttttc
     2221 aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac taaagcgggc
     2281 aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt ttgcttgaag
     2341 tttagccgct agttgactag attccaacca aaaatcataa tttcctacat aaagtttgat
     2401 tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt gacggtcatg
     2461 ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc aattgatcga
     2521 ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt taccaaatag
     2581 tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca tttgctgatg
     2641 gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg gttcagcttc
     2701 ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac catcttcgtc
     2761 agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat acagacgctt
     2821 gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat ggttctgttt
     2881 caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag gctgcagatc
     2941 ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa tcaagccata
     3001 gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt ctgaaaagtg
     3061 caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt attagttttg
     3121 acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc gaacattctt
     3181 tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga aaatagctac
     3241 tataggattt attagtttgc aacagcataa tttttgacgt tatttataag agggagtact
     3301 ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga accatcgact
     3361 ggggctcatt gattttggga attttatttg tacttgtctc attgttatca tttcaagacc
     3421 ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt attaaaggga
     3481 tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa ggaaagatgc
     3541 ccttaataat cgggatcatc gatattctag ttggcgtctt tttcttattc aatattggtg
     3601 caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat tcagttttag
     3661 cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg tttacgatca
     3721 tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca atttcttctg
     3781 cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc acacatatcg
     3841 tctatgcatt tagataagta aaatcaaaca aataaaaaag ccggcttccg attacagttt
     3901 tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga ggtgattttt
     3961 aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat tttatcttaa
     4021 tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc aatactggca
     4081 aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa tattttcttg
     4141 gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc acatcgatta
     4201 ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc gagtattctg
     4261 tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct gcatcttcta
     4321 aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg aaaccaaaag
     4381 aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt gtaatgatat
     4441 cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt ttttctgggt
     4501 agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg aaccatccag
     4561 atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg atagtcgtgt
     4621 taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt tctttataga
     4681 cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac caatttaccc
     4741 atttttcttt attttcttga tttgctaggt accagctatc agcagggcga gcatccccta
     4801 actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa gatgtttcat
     4861 ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca gttggattaa
     4921 aaaatgcacg tccaaatggg aagtcgctac cgacaaataa gatcagatcg gcttctgcca
     4981 atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag ttttcataat
     5041 catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa aaatgttctg
     5101 agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa agaactggtt
     5161 tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct ggtaatatca
     5221 cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct tcaaaaccaa
     5281 gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct ttaatggctt
     5341 catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat acgctaacat
     5401 ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg gatgtagaag
     5461 caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca tacaaaccat
     5521 tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact ttccccgtta
     5581 attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact tgaatgtagt
     5641 tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat cctccgggaa
     5701 tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg attcctgcat
     5761 tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt ttttgtatct
     5821 agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa aaatgtagtc
     5881 atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg gaatggcatg
     5941 gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac agcttttact
     6001 tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat caaaaaagaa
     6061 gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag tttttggtct
     6121 ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg gctagttgta
     6181 agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac actgccccat
     6241 atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa acggacgatc
     6301 ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt gggggtagca
     6361 ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc tgtttcggtg
     6421 gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat cccgttgcga
     6481 caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg aatcgttgaa
     6541 ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct gcttccttat
     6601 gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt gatccctgaa
     6661 gcacagcaga cattgtccag caaacatcat tttgcagttt ttggcgtcat gagtggattt
     6721 atcattatga tgattttgga tgtggcatta ggataaataa aaaaaatata tatgatatat
     6781 caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat atcataatga
     6841 agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt tgatccagcg
     6901 aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc ataaaagccc
     6961 gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca ctaaaagaac
     7021 catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc ttgttcattc
     7081 tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg aagactgatt
     7141 tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat catctaaaat
     7201 agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt tacagatgct
     7261 gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa cggagacacg
     7321 tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag ttttatcatt
     7381 ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa gataaatgga
     7441 cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc gtttgcaact
     7501 tcatggatca gtgagtacat caatagcctg gctccagaaa tgacttatgg ttttgttacc
     7561 ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc aaataaaggc
     7621 cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct atcgattggt
     7681 ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt gctgatcgca
     7741 aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc acaatatcgg
     7801 taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat aaacgatcaa
     7861 atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa gaattcatgt
     7921 aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc atttgacttg
     7981 gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa aataaacatc
     8041 tttatgtaat ctgatgttct atctatttaa gaaaagcatg tttacagcag tatgattctg
     8101 ttgtaaacgt gtttttattt gtggaaaatt taatttttag aaaagtgtat cgaaaaatag
     8161 ata
//
LOCUS       DAYYAR010000119         8121 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000119 DAYYAR010000000
VERSION     DAYYAR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.121"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..634
                     /locus_tag="ACHAT0_002340"
     CDS             26..634
                     /locus_tag="ACHAT0_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8452253.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            802..2439
                     /locus_tag="ACHAT0_002341"
     CDS             802..2439
                     /locus_tag="ACHAT0_002341"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8452254.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(2511..3623)
                     /locus_tag="ACHAT0_002342"
     CDS             complement(2511..3623)
                     /locus_tag="ACHAT0_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8452255.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(3698..5071)
                     /locus_tag="ACHAT0_002343"
     CDS             complement(3698..5071)
                     /locus_tag="ACHAT0_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8452256.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5292..5909
                     /locus_tag="ACHAT0_002344"
     CDS             5292..5909
                     /locus_tag="ACHAT0_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8452257.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6007..6462
                     /gene="argR"
                     /locus_tag="ACHAT0_002345"
     CDS             6007..6462
                     /gene="argR"
                     /locus_tag="ACHAT0_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8452258.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            6480..7982
                     /locus_tag="ACHAT0_002346"
     CDS             6480..7982
                     /locus_tag="ACHAT0_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8452259.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
BASE COUNT         2783 a         1540 c         1689 g         2109 t
ORIGIN      
        1 aaacggagaa aaaaaggagt ttaatatgaa ttcatttaag aaaattgttt taggtacaac
       61 ttttgctgct ggtgctactg caatgtttgt aggaactaca aatgctcacg ctgatgaagt
      121 ttatacagta aaatcaggtg attcattatc taagatctca caaaaatttg caggagacaa
      181 ctcaatgatc gatgcaattg ctgaaaaaaa ttcgatcgca aacatcaacc gcatttatgt
      241 tggtgaacaa ttaacaatcc caacttcaaa tgattcttca gcaacaacag aaaacaaaac
      301 aacagaaaat acagcatcta caactgaaac agcaacgcaa gaacatacat acgttgcacc
      361 tgttgaaaca gttgaagtag ctcctgctgc cccagctgct gcaacagcgc caacttcaag
      421 ctctgcaaaa gaatggatcg cacaaaaaga atcaggcggt tcatacacag ctactaacgg
      481 acgttacatc ggccgttatc aattagatgc ttcatactta aacggcgact attcagctgc
      541 caatcaagaa cgtgtagcag aacaatatgt aacatctcgt tatggttcat gggatgctgc
      601 taaaacattc tggttagcaa acggctggta ctaagccgat aaaaggaaaa tgatgaaacc
      661 gagaaacttg tttattcaag ttctcggttt ttttgtgtga aaaataactc gtttgcttcc
      721 cacagcgcag cagtacaaat aaaaactcga ataatgtata ataaagaaaa cgtttttttg
      781 tttagggaat gaggaggagc catgagtaca aaagagagtc tcgaattaaa cgaaatcaca
      841 agtggaaatc cactgttatc cagtattcga tcgattgtag aaacgacatt ttacggtaat
      901 aatgttcatg aagtttttga taggaagaca gcttatcaac tagcaaaagg gagtccagga
      961 acgatcatca cagatttaac aatctcacat gctgaagaac tagatttacc agctgatgtt
     1021 aggacactag tattcaatga tggaagcatt gtgggaagaa cagcaagtgc tcggcggatc
     1081 tttgaggact tagacaagga acaaagcaaa tacgagaaaa ttttaagaga agctgtgtat
     1141 caaagtcgca agcgccagtt ttaccataca aaagtaatcg ttggcctatc tgaagaattt
     1201 tctgtgcaaa gtcatttgct gattccagaa ggatttgaga acaatttgta ttcttatttg
     1261 ctgaattttc agccagcgaa ttcgaagatc ttagagactt atcaaaagtc tgtttccttt
     1321 gatgagggag atctttatat cttttgtgat cccgaatgga cacatgaaac atttccaaat
     1381 ggactcattt tgtttgatgc tctccataat acagcggctg tgctaggatt acgttacttt
     1441 ggagaattaa aaaaagcgac tttgacgctt gcttgggcat tgggaaatcg caatggcttt
     1501 attgcctgcc acggtgggat gaaacaatat gctagtaatg accagaccta tacgatggct
     1561 gtatttgggt tgtccggttc tggaaaatca acgattacct tagcaaaaca tacggaaggt
     1621 cactctgttt cgatcttgca tgacgatgct ttcgttattt ctcgaaaaaa tggttttgct
     1681 actgccttgg aaccttctta ctttgataaa acacaagact atccgtctga tgatccggcc
     1741 atttcttctt tccttacttg tcagaatgtg ggcgtgacat gtacaagtaa aggagaaaaa
     1801 gtcctagtaa caggagatat acgaaacgga aatgggcgga cagtcaaatc acgttatgcg
     1861 acacccgacc gagtcgatca tcttagcgaa aagatcgatg ctgttttttg gatcatgaaa
     1921 gatgattcgc ttccgccaat tatcaagatc aatgacccag ttttagcagc gtcttttggt
     1981 gcaacattag caacaaaacg atcagacgcg gaaaatatca ctacaaaaag acagctagat
     2041 gagctagtca ttgaaccatt tgctaatcct ttccgtgtct atcctcttag ggaagactat
     2101 caagcttttc gagagttgtt tttgaaacag caaacagcct gctatgtcct gaatactgga
     2161 tatttcgatg ataaaaaaat caaacctgaa acgacattgt caatcgttga aagaatcgtg
     2221 caacaaacgg cagaattcaa gtcgtttggt ccatttgaag acatccacta tttacctata
     2281 ccagattttc ctgtttcttt tgaggatacg tcctataaaa atctggtgaa acaacgactt
     2341 gatattcgtt tacgattcat tgaagctcag ataggaaaag atctcttgcc agatgaagca
     2401 aaagaaagtt tggagaattt gattcagaaa ttgaactaaa ataacagctt tttcaagaag
     2461 taatccatgc gcaatatcgc tcgggatcct tacttgaaaa agctgttttt ctatgatgga
     2521 tttttcgtaa tataggtgga gatgtccttt tgataatctt ccgtaggcca atgaccagta
     2581 tttaagaagt tggctaatag atagccgata taaggacctg tggtcaagcc agaagagcct
     2641 agaccgctag cagccagtaa gcttggatca tctggcagta cgccgaaaaa tggggaaaaa
     2701 tctgacgtat aagcacgggt acctacacgg aactcgtatg gccagttttt tatctgatct
     2761 ggatcagtca agaactgttc tgtactctta gtcaatccct tataagcttc gctagtagga
     2821 gccaaatccc agccttgatc gttttcatgg gtagcaccta aaaggatctt tccatccatg
     2881 aaaggaatga aatcagcttc tccgtcaagc atagcaacag gccagtcttg actcatctta
     2941 taagatgtct gaaatgctaa gagctgccct ttttgcggac gtatatctgc agcatagcct
     3001 aattcttgta ataaaggctt aagtgccggg ccaggtgtca aaattacacg atcagctgtg
     3061 tattctccaa acggtccctt gatgagccaa gaatcgctct tcttatgcaa agtgactttt
     3121 gcctggatct gctccatttg tttttgtgct aaacgctgac caagatgttc taaatatgct
     3181 tttccatcca gacgaccacc accactgata cgcagtgaaa ctgctggttt caataacgga
     3241 agtgcttgag atgtctgttc aggtgaaagc cattcgatgt ttccaatttc tggcgcttct
     3301 tcttttcgtt tctcggcaag aacagccaat tcagctaaat ctttttcagg tcgaagcaaa
     3361 agtgtccctg attgtgcgta aatcgtttga tcaagttgga aatcttttat aagtttaggg
     3421 aaaaaagcag cgccgtcttt agccagacga taccattgtt tgtttctgcg tttcgacaac
     3481 caaggggaga taatcccagc gctagcttta gttgcctgtc ctgtcccttc atcaaataaa
     3541 gcaacattga attttgatgt atctaagtaa ctggctaggg tcataccaat gatcccgcca
     3601 ccgataatgg caattttttc cataaaaaag cctcctttta caaacaacca ttttcacaca
     3661 aaaaaacggc agatgcaaca cctgccgtat atggaatcta cgctttttta gcaaagaaca
     3721 gataataggc gaatcgagca aagcttacga gaagcaacag caacaagacg cctaatgctg
     3781 cttttgatcc taatgtagta gacacttcat agttatggga agtatgatgc tggacgtaat
     3841 tgataatgac cgctactaat gaagtagcca cagcgccaga aaactgctgc aaggtattga
     3901 acattgcgtt cccgtctcct tgtagttctt cattcagtgc attcattccg gtggtcatca
     3961 tattgctgta agataatccg ataccgatca tataaaacac atgacaagca atcaatacac
     4021 ccactgaagc gtgtcctatg agtaggaata atgctagcca accaatcaaa gaaagaatca
     4081 gcccagcaaa aattggcttc ttcggaccat attgatccag cattttcccg gaaaaaggag
     4141 aaagcaaagc accaaccgct gcgccgggaa gcatcgctaa tccagcgaca aaaggactac
     4201 ttccttgaac gatctgaagg aaattcggca agacaaaaga tacaccaagt aataaaaact
     4261 gataaactaa gaagccaatc aaaaacaagc ggaatgtttt atttgaaaga atagacagtc
     4321 ggatcagtgg atgctccact tttttacttc gatagtaaaa aaggaaccag ccaataccgc
     4381 cgatgattaa agcaatccag ctggaaactg ttcccaacgt actcaaaaat gttagacttc
     4441 cactaaacaa aagacaaatt cctaaaacgc ttatccaatc caaataacca cttttttgta
     4501 ctgccatctc gggaatagca aaccagccga taatcaaaga aagaaacaaa acgggcacaa
     4561 gaaacaggaa gatatgattc catgtaaaat gtgaagtcat aatgcctcca taagtggggc
     4621 caattgctgg agcaatggcc gttgtcaaag taccgacacc catcatcgcg cctcgtcgat
     4681 tgaggggggc aaatgtcaag ataatatgga acatcaacgg aagagcgata cctgtcgctg
     4741 caccttggag caatcgacca aacaatagga ccatgaaggt aggagagaag aaatcgatca
     4801 ttatacctgc caagaaaagc agatttgaag ctaaaaagag ttttttgaga ctaaaattgc
     4861 gattcaaata agtagacagc ggaacaatga tcgaaatcat caaaagataa atcgttgtta
     4921 cccattggac ttgtgcagtc gttaaatcaa actgtctgat aagagttggg aaagttacat
     4981 tcatggccgt ctcgatcaat acgcccgcaa aagacatcat ccctgttgca attactgcag
     5041 gtactaaaag cagctgtttt tgttttgtca tttacatcac ctctaaaaaa tttcgtaaaa
     5101 aaagagagtg atttctatta taaaaataga aaccgcctct ctctcgacac gccgaaacgt
     5161 gttatctgta cgatttattt acttcactta ttctagcaca aactgaaaaa tagtgtcaac
     5221 acgattagcc aagtcactca aaaagaggta tgataataaa taactaaatg gaatttcgcg
     5281 gaggaagaaa aatggaaaaa attgaatggc gtaaagacaa gagctatagt atgaaaaaaa
     5341 gcccgcaaat actagaggtt cctaaaaaat cattctttag catcagaggg atcggcgacc
     5401 cgaatcagga agactttaaa cgaagagtag cctgtctata tgcagtcagc tatgcaatac
     5461 gcatgtcgcc taaaaaaaat tggctgattc ctgattatca gatgtatact gtctatccac
     5521 tggaaggaca atggggactg caagaaaagt atttgcatga accagtgatg aaaaaagaac
     5581 atttttctta tcaattgatg atccgacagc ctgactttgt tacagaagcg atagcacaag
     5641 aagccattca gcgttctagc aaacttccgg aagacttgaa agaacaagta gtattcaaaa
     5701 agatggaaga aggtctttgt gcccaaatct tacacgtcgg gccctatgcc gaagaaccag
     5761 caacgtttga gaaattagaa gcattcatcg caaaagaagg ctatgaaaga gcttctaaag
     5821 aacacaaaga gatttacatc agcgatccaa gaaaatcagc acctgaaaaa atgaagacga
     5881 ttttgcgtgt taaaatagaa aaaggttagc aacctgaatg aaaacaaaaa ataaaagtaa
     5941 aaaataccaa aatatgaaga aatattactt atcttttgct ataatcaaca ttgagaggtg
     6001 tctaatatga aaaagaaaca gcgacaagca ctgatccgcc agatcattac agaacaacct
     6061 attgggacac aggaagagct gcttgcgcgg ctgcatgaag caggcgccga tgtgactcaa
     6121 gcaacgattt ctcgagacat tcgagaaatg aagttagtaa aaagtcaaaa tgaaaacaaa
     6181 atagtacgct atacattatt taaccagcca tctgtttctt taaatgaaga acgtttgcgt
     6241 acagcaatta gacgcgaggt tttgcgtatc cagtctgttc agttcatggt tatcgtattg
     6301 actgaacgaa atggtgccga tgtggtaacg aattggctag atgaagtggc ttaccctgaa
     6361 gttgtcggaa cgatggccgg tgtagataca ttcattatta tttgccgctc agaagaagaa
     6421 gcacagcgat ttgcagaaaa gctggaaaaa atgagggaat aaatgaggag gacttttata
     6481 tgaaagatat gaaagcgctg caaaatggat cggatattcg aggaatcgca ttaccagttg
     6541 aaggatatga agtaaacttg acgacagaca tggtaaaaaa gatcggctgg ggactagcaa
     6601 actggataaa gaaaaagaag aaatcaaccg atccaaataa aaaatggacg attgggattg
     6661 gtcatgacag taggataagc ggaccagcac tgaaagaggc gttgatccaa agtctgaaag
     6721 aacaagggat acatgtcatc gatttcgaat tagctactac gcctgctatg tttatgtcca
     6781 ctcaatttga acagttctcg tgcgatgctg cggttatgct gactgctagc catcttccct
     6841 attattataa tggaataaaa ttctttacct cagaaggagg agcagaaaaa gaagatattg
     6901 cttttatcct atcacagcag caaaaaataa aaagcgaaca aactggaagc gtagaaaaag
     6961 cagacctttt gacaccttat gcgcaagatt tagtcacaaa aattaaaaag gggattggaa
     7021 cactagaaag caaaccacta aatggttgga aaatcattgt tgatgccgga aatggtgctg
     7081 gtggattctt tgctgaaaaa gtattgaata gcttaggtgc ggattcaaca ggttcccaat
     7141 ttttggaacc agatggccga tttcctaatc atatcccaaa tcctgataat aaagaagcga
     7201 tggaaagtat caagcaggct gttttagcaa atgaagcaga tcttgggatc attttcgata
     7261 cagatgtgga tcgttctgca gttgtcagtg cttctggaac agtgatcaat cgaaataatt
     7321 tgatcgcagt attaagtaca atcgtattaa aagagcatcc aggaacaaga atcgtgacga
     7381 attcccctac ttcttctcat ctacaaagat tcattgaaga aaaaggaggc atccaagttc
     7441 gttatatttc cggctatcga aatgtaatca ataaagcatt agaacttaac cgtcttggga
     7501 aagatacaca gctagccatc gaaacaagcg gacacgctgc attcaaggaa aactattttc
     7561 tggatgatgg tgcatatgtc atcgcaaaga tcctgatgct tttgccagaa ttaaaaagaa
     7621 aaggtatcac attagatgaa caaatcaaag agttgaaaca gcccgtagaa gtacaagaaa
     7681 tccgattaaa attgttcagc gatgaaccac gcgaacaagg aaacaagata ttagaagaaa
     7741 tgaagcgact gttggaagaa gaaaaaggga tgaacttcga cccggaaaat gaagaaggta
     7801 ttcgatatga tctagaagag ccatacggaa acggctggtt tttgatccgt ttaagcttac
     7861 atgagccgtt actagtatta cagatagaaa atgatgaatt cggaaagaac aagttggcgt
     7921 tagtaaaatt agcaaaatta ttcggacatt tttctgatct tgaatcatta acagcagaat
     7981 aaaccagtct ttttgaaagg ctagttgctt ggacggcaaa aaaagtggta tggttaaatc
     8041 gagtgctggt agggagacca gcaaaaggag gaaaaaatgg taacgaatat ggttattttt
     8101 ttaacagtat tggtttatgg g
//
LOCUS       DAYYAR010000120         8101 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000120 DAYYAR010000000
VERSION     DAYYAR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.122"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            382..570
                     /locus_tag="ACHAT0_002347"
     CDS             382..570
                     /locus_tag="ACHAT0_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8452260.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            648..1661
                     /locus_tag="ACHAT0_002348"
     CDS             648..1661
                     /locus_tag="ACHAT0_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8452261.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1728..2762)
                     /locus_tag="ACHAT0_002349"
     CDS             complement(1728..2762)
                     /locus_tag="ACHAT0_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8452262.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2877..5102
                     /locus_tag="ACHAT0_002350"
     CDS             2877..5102
                     /locus_tag="ACHAT0_002350"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8452263.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5120..7003
                     /locus_tag="ACHAT0_002351"
     CDS             5120..7003
                     /locus_tag="ACHAT0_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8452264.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6984..7958
                     /locus_tag="ACHAT0_002352"
     CDS             6984..7958
                     /locus_tag="ACHAT0_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8452265.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2614 a         1476 c         1708 g         2303 t
ORIGIN      
        1 gttagtctac attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa
       61 tctgggtcag aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact
      121 gattgatcaa aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg
      181 tttgacgcgg aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga
      241 cccattattt tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg
      301 ctgggagttt tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg
      361 actgtaacga aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa
      421 gcgacacgat atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca
      481 acagaagaag aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca
      541 ttgcagaaga agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac
      601 actatggtat aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa
      661 ttcttaaatt tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc
      721 ttgccttcca gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata
      781 ctgttcaaat cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa
      841 cagatttgac gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa
      901 acagcaaggg gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag
      961 ataaatcggg tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg
     1021 ttgcgatgaa ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga
     1081 atgaactgat caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac
     1141 aattattttt tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac
     1201 aaacgaatga tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc
     1261 tgaaaggaac catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt
     1321 cggatgatcg atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa
     1381 aagattggaa aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg
     1441 aatttccgcc aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag
     1501 cattagaaaa tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata
     1561 caaaaaaaga gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt
     1621 gttaccagca gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt
     1681 ttcgttttga gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt
     1741 gttcttggga taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt
     1801 tcgtcccgta taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat
     1861 ttcgcattga tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta
     1921 tcaaagcttg ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga
     1981 tagacaccaa ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca
     2041 ttttctaata gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga
     2101 taagacatat atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc
     2161 ttttcattta tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg
     2221 atataggcta ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc
     2281 cgttcaagat ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg
     2341 actatttgca tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca
     2401 acgatcaccg cacctaactg atctagatct tcccattgtt taggattatc agatagatta
     2461 tagattcgat tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa
     2521 tgtttgtaat agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc
     2581 gaaggcattc tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct
     2641 gcctctacta tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac
     2701 acacgggaaa ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc
     2761 atttttacac actcctcaaa aaaataaaaa atttttattg acaaaattgt ttagtaaaca
     2821 gtatacagga attataaaaa aaagtaaacg gtttccgttt tgttgggagt gaaatgatgg
     2881 atattacgca tagaaaaaca gataaaaatt tcatcttatt tgatgaaaag agccgagtct
     2941 tccatttacg aaatacattt ctttcttatc tgataaagat agaagaaagc aatgtattgg
     3001 cgcatattta ttttggcaaa ccagtcaagc agtacaaaga caataaaaat tatccccgaa
     3061 gagatcgtgg attttcagga aatgttccct taaatcctga ccggtctctg tcgaaagata
     3121 cactaccgca agaatactct tctcatggca gcatggactt tcgaacgccc gcatcgatca
     3181 ttcagcgcaa gaatggatca gacttactgg atctgagata tgacagccat tatattacag
     3241 atgggaagcc agagatcgaa ggcttgcctc aaacttatgt gatggacaag tcagaggcac
     3301 aaacgttggt gatttcactt aaggatagag aaacagcgat ttatttcgat ttgttctata
     3361 ccatatttac tgatagagca gtgatcacac gatctgtaaa gataagaaat gaaaccggag
     3421 aaacaatcaa actagaaaaa gcggctagtt tccagctaga ctttgcccat actaggcgat
     3481 ttgatgaagt cattgctttg ccaggagcac atgtcaatga aagacagatt tcacggcaat
     3541 ctgttttatc tggaacaaaa gtatttgaaa gccgtcgagg aacgagcagt caccatatga
     3601 ataactttat tgctttagtt catcatcata cgacagaaaa tactggagaa gcgatcggac
     3661 ttcaatttgt ttattccggc aaccattctt tcgaacttga aaaagatcag atcaatcaat
     3721 tgagagtcgt cggtgggatc aacagccatc gtttttcgtg ggaattgaat gcaggccagt
     3781 cttttcaaac acctgaaatg atcctttctt actcaagtca gggattgaac aaaatgagtc
     3841 agatccacca tgagttgcta cgtgaaagaa tcgcaagagg tcgtcatcaa tttgctgaac
     3901 ggccgattct ggtcaataac tgggaagcca cttactttga cttcaatagt gaaaaaatca
     3961 aggcgatcat tgatgaagca aaggagctag gcatcgaaat gttcgtatta gatgatggct
     4021 ggtttggaaa aagagatgcg gacaattctt cattaggaga ctggttcgag tatgaaggca
     4081 agttgacaaa tggattacgt gaaatagcag attatgctca tagcaaagga ttgaaatttg
     4141 gtttatggtt tgaacctgaa atgatctcgg ttgattctga actgtatcga acacatcctg
     4201 attttcttat gcaggagcca ggtagaatgc cttctgcttc cagatcacaa catgtactgg
     4261 attttacaag gttagatgtt cggcagacga ttgagaaaca aatgagaaag attttagata
     4321 cgattcctct tgactacatc aaatgggata tgaaccgttc gctttcagat gtctattcga
     4381 ttacccttga cccacagaga caaggagaag tagcgcatcg ttatatgctc gggctttatg
     4441 aactacttga acatttgaca actgattatc cggaaatctt atgggaaggc tgttctggtg
     4501 gaggtggacg gtttgatgcc gggtttattt attatatgcc gcagtcatgg acaagtgata
     4561 acacggatgc agtggagcgt atgaaaatcc aatatggaac aagccttgct tatccgattt
     4621 cgtccatcac agctcatgta tcagctgtac caaatcatca gaccgacaga tctacttcgt
     4681 tgaaaacaag aggagaaaca gctatgagcg ctgtttttgg ttatgaactg gatttgacaa
     4741 agctgtctcc tgaagaaaaa aagcaagtaa aagaccaaat tatatcctat caaacgattc
     4801 gtccggtgat ccagtatggc cattattatc gtttggcgag tccttttgaa gaaaatattg
     4861 ccgcatggat gtttgtgtct cctaaacaag atgaagctat tgtgttttta gggaggatac
     4921 tagcttcagc tcaaccagcg ttccatgaag tatatctgat ggggttagat gatgaggcac
     4981 tttatcagga acagacctcg aagcggatat tttcgggggc cgaattgatg acagttggac
     5041 tttacttccc cgattttcaa ggtgatttcc aaacagaact gcttcatttc aaaaagttat
     5101 gagagagaag gaaaaaagta tgaaagcaaa tataaaatgg ttagatgatc cacaagtttt
     5161 tagagtgaac caatcaaatg cacatagcga tcatgtttgt tatgcaacaa gggaagaagt
     5221 agaaaccaca tcctccctta aacagtcttt aaatggatca tggcgttttt gttattcgcc
     5281 aaatccttcg gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga
     5341 tgaaatcact gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa
     5401 tacgatgtat ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga
     5461 gaaagaggcg gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac
     5521 gacttttcga ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt
     5581 agaacaagca atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt
     5641 cactccgtct gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga
     5701 ggtttacaaa agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg
     5761 gatcttcaga gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat
     5821 caaacctcgc ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga
     5881 gcaagtagcc aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat
     5941 ctatcaacag caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa
     6001 tgtggcactg tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga
     6061 cgaagaaaaa agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat
     6121 caaagataaa atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca
     6181 tgagtggcat ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc
     6241 tatcataaaa aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac
     6301 ttggtattat ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc
     6361 ccatggctca tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt
     6421 acctcagtgg caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa
     6481 ccatccaagt attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg
     6541 acaaatgaac gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa
     6601 ttttccaaat ccagcattcg aagattcgat ttcagatgta aaaagctgca tgtatgcgcc
     6661 accagaagag atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga
     6721 atatatgcat gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga
     6781 tcgttatgaa atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt
     6841 gactgatcca atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc
     6901 aagtgactat gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc
     6961 catgcaggag gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg
     7021 aaaaagttgc aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt
     7081 atattttttc ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc
     7141 tataccggga aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg
     7201 gatttggatt taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa
     7261 tagaagtact ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat
     7321 attcaagtaa tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag
     7381 taccaaaaac ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca
     7441 atatgcatta ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca
     7501 ttatgccaac gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg
     7561 atcggatggc tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat
     7621 accttgttcc ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa
     7681 atcaaacgtt ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc
     7741 aggatacgcc atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa
     7801 cacatataga agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa
     7861 gaggagtcgg gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca
     7921 gcgaagagga ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga
     7981 aaaacggagt ttacaagaca aagctgcttt attctatatg tcatgaacaa gagagtaaaa
     8041 aagttctcag atacttttgt tcatgactat tttcttcaat aaaaccaatt gttatttttc
     8101 t
//
LOCUS       DAYYAR010000121         8096 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000121 DAYYAR010000000
VERSION     DAYYAR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.123"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1458
                     /locus_tag="ACHAT0_002353"
     CDS             4..1458
                     /locus_tag="ACHAT0_002353"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFM8452266.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1534..2694
                     /locus_tag="ACHAT0_002354"
     CDS             1534..2694
                     /locus_tag="ACHAT0_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFM8452267.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2807..3814
                     /locus_tag="ACHAT0_002355"
     CDS             2807..3814
                     /locus_tag="ACHAT0_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8452268.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3838..4863
                     /gene="iolG"
                     /locus_tag="ACHAT0_002356"
     CDS             3838..4863
                     /gene="iolG"
                     /locus_tag="ACHAT0_002356"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFM8452269.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4880..5770
                     /gene="iolE"
                     /locus_tag="ACHAT0_002357"
     CDS             4880..5770
                     /gene="iolE"
                     /locus_tag="ACHAT0_002357"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFM8452270.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5793..7388
                     /locus_tag="ACHAT0_002358"
     CDS             5793..7388
                     /locus_tag="ACHAT0_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFM8452271.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7404..7718
                     /locus_tag="ACHAT0_002359"
     CDS             7404..7718
                     /locus_tag="ACHAT0_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8452272.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
BASE COUNT         2409 a         1534 c         1939 g         2214 t
ORIGIN      
        1 taaatgacaa gatttttaag aaattatatt gatggtcgtt gggtcgctag tcgctcaaca
       61 gagtttgaag aagtaatgaa tccggctaca gaagaagcgc tggcgaatgt accgttgtct
      121 agcaaagcag agacagccga agcagttaaa gctgccgatg ctgcgtttca aacttggaaa
      181 aaagttcctg ttcctgttcg cggacgttat ctgttcaagc tgcatcagct tttaacagat
      241 cataaaagtg aattggcgcg tttgatcacg ctggaaaatg ggaaatcatt gtccgagtca
      301 ctgggagaag ttcaacgagg aatcgaaaat gtggagcacg cagcgggaat caccaattta
      361 atgatgggag attctttatc aacagcggcg actgatattg agatcacgaa ttatcgttac
      421 ccaatcggag taatcggagg gatcacaccg tttaattttc caatgatggt gcccttctgg
      481 atcttcccga tggctctggc ggcaggaaat acggtggtgc tgaagccttc agaaaaaaca
      541 ccattgttga tggagaaaat tgttgagctg gttgaaaaag caggctttcc tagcggtgtt
      601 tttaatgttg tctatggtgc ccacgatgtt gtcaacacat tgctgcaaga tccactggta
      661 aaaggcatca gctttgtagg atcaaaaaat gtcggtgaat acgtttaccg tgaaggaaca
      721 aaaaatctaa aacgggtaca agcgttgact ggagcgaaaa atcatgttgt tgtgctgaaa
      781 gatgccaata ttgatcgggc agtcaaggac cttattggcg gagcttttgg ttctgctggt
      841 gaacgctgca tggcagcctc cgtcattgtt ttggaagagg aaatcgcgga tcagtttgtt
      901 gaaaaattta aggctgcggc aaaagcgatc aagatcggca atggcttgga cgaaggggtc
      961 tttttaggac cagtgattcg aaaagatgca cagcagcgga cctttgacta tatccaaaaa
     1021 ggggtcgatg aaggagcgaa actggtacta gatggtcgcg aaaacattcc agaaaaaggc
     1081 tattttgttg gaccaacgat ttttgataat gtcacgatgg atatgaagat ctggcaggat
     1141 gagatttttg ccccggtcgc atcaatcgtg cgggtaaaag atttgatggc agccgttgag
     1201 ctgacgaatg aatctgaatt tgcgaatggt tcgtgtctgt ataccaacaa cgcatcagcg
     1261 attcgctact tcagagaaaa tgttgatgct gggatgctgg gggtcaatct tggggtgcct
     1321 gcaccgatgg cttggtttgc cttttcaggc tggaagcact ctttctttgg tgatttgcat
     1381 gccaatggca aagacagtgt tgagttttat acccatcgga aagttgttac agcacgttat
     1441 gcgcaaagtg agatttaaag atttgcttcg aagctctgtt aaagagcttc gaggattctg
     1501 ttataggcta taagccagag agaggttttt tctgtgaata ttcaactgaa aatgcctgcc
     1561 aatttaagaa ttggcggcgg aacaatccaa tttcttatgg aagaatcaga acaactaggc
     1621 aacaagccgt taatcattag tgatacggtt atggaacaga ccgggcatct tcaaaagatc
     1681 aaggagctgt tgggaaaaac agatgatcaa ttagcggagt atacagacat tcaagccgaa
     1741 ccgacgcaag aaaatgttta tgatgcgctg aagctttttc agaagcatca atgtgatcat
     1801 atcattgctt ttggtggcgg cagctgtctg gatacagcca aggtggtagc cgttttggct
     1861 gctaatcaat taagtctcga agcgattcat gcaagaaatt ttgatcagct gcaagcgggc
     1921 attccgctga ttgcagtgtc aacaactgct ggaactggtt cggaagtaac cgatgtggca
     1981 gttattacca atacaaaaac agcagtcaaa atgatgatga aggatctcgt tttcatgcca
     2041 cagatctcaa tcgttgactc tgattttacg aaaactgtac caccaaaagt aaccgcggca
     2101 acgggagtcg atgcgctatg tcacgctttg gaatcttatg tttcagtcaa agccaatgaa
     2161 gtaacgcaac tattttcact ggaagcgatg aagaaaattt tgaacacgct ttctcaggca
     2221 tatgaaaata gcgacgacac tgaagccaga gaacaaatga gcttagcttc aacggaggcg
     2281 gggattgcct tttcgaatgc gtcagtcaca ttgattcatg gaatgagtcg accggtgggg
     2341 gcactttttc acattcctca tgggatatca aatgcgatgt tattgccggt cttttttgaa
     2401 ttctcaaagg atgctattca gactgagtta gcgacgatcg ccagatttat ttatccggca
     2461 gccgaagatt tatcgctgga agaggctgcg gattatctga ttaaaaaaat ccaagagctc
     2521 tgtgagcagc tgtcgatccc aacgattcga gattatggta ttgatcagca agcgtttatg
     2581 gcagccttag acaaaatggc cgcggatgcg ttgaccagcg gaagtccgca aaataataaa
     2641 aaggtgccta gtcatagtca aattaaggaa ttatacgtac aagcttatgg gtagtaaaag
     2701 gcggagcaaa gagagggctc taccttttaa gaataaaagc tacacaggaa taattttgta
     2761 agcgcatact atgtgtgctt aaacaacaga acagggggat aagtaattga gagtaaaagt
     2821 aggcgttatc ggaacaggtg caatcggcat cgagcatatc gacaggatca atcagaaaac
     2881 acaaggggct tatgtgacag cagtctcaga tatcaatgta aaagctgcta aagaggttgc
     2941 caaacgtgtc gatgctaaat tctttgaaac cggagaagca ttgatcgctt ctgaagatgt
     3001 ggatgtcgtt gttgtgactt cttgggaccc gacccatgag cagtatgtgt tagaagcaat
     3061 caagcatggg aaatatgttt tctgcgaaaa gccattagcg atggatgcag caggctgccg
     3121 ccggatcgtt gatgcggaag ttgcttatgg gaagaaattg gttcaggttg gcttcatgcg
     3181 ccgctatgac cgcggctaca ttgaactgaa agaagcaatc gaatcgaagc aatacggaga
     3241 agcattaatg atgcattgtg cccatagaaa tcctagcgca gatgaaaact acacaacacc
     3301 gatggcgatc agcaacaccg cgattcatga gattgatgtg cttcgctggt tgctaaatga
     3361 agattatgaa tctgttcaaa tgatcttgcc gaaaaaaacc agcaactccc atgccgattt
     3421 gcatgatccg cagctgctga tctttcaaac aaaatccggt gtaacgattg atttggaaat
     3481 ctttgtgaat tgccgctttg gctacgatat taaatgtgat gtggttttcg aaacggcaga
     3541 agtaggaatg acggacccag cctatacacg aattaaatcg gaagaaaaaa attacacacc
     3601 tgtttcacca gattggcaga cccgcttttt ggatgcctat gattatgaat tcaagctgtg
     3661 gatcgattcg atcaaaaatg atgcggttgt cggtcctacc gcttgggatg ggtatgtagc
     3721 agcgatcacc atgatggctt gtcatgagtc tcgtgaaaca aaaacaaaag taatgattca
     3781 aatggatgaa cgaccagcac tatacaatga gtaattctca gaaaacggag gtatttcatg
     3841 aacaacatta gagtaggcat cgtcggttta ggaagattag gaaaagtaca tgcacaaaat
     3901 ttagcaacag gtgtcagttg ttgtgagctt acggctgcgt gcagtttagt tgaaacagaa
     3961 ttagcattcg cacagaagaa attaggcgtc aagcatactt tttccagcta tgaagaaatg
     4021 gtcaaaagtt ccgtgatcga tgcagtcttt attgtttcac catcaggctt tcactgtgac
     4081 caagtccgct tggcgatgga aaatggcaag catgtcttta ctgaaaaacc attaggcctt
     4141 gaagttgaag atattaaaaa gacccaaaaa gtcatcgaag agcatcctga acaggtcttc
     4201 atgttaggct tcatgcgccg ctatgatgag tcgtatcagt atgcgaagaa aatggtagaa
     4261 gatggcgaaa tcggcgaaat cacactgatt cgttgttatg ggatcgatcc gagcgaaggc
     4321 ttggaaagct ttgtcaaatt tgccatgaat aacgacagcg gcggcttgtt cttagatatg
     4381 gcgatccatg atattgattt ggttcgctgg tttaccggcc aagaagtcga tcaagtgtgg
     4441 gcgattggga aaaatgcggc gtatccagaa ttggatcagg ctggtgagct tgaaactgga
     4501 gccgcaatga tgagcctgag cgataaaaca atagctttac tagttgccgg cagaaacgca
     4561 gcccatggct atcatgtcga aacagagatc atgggaacca aaggaatgct gcgggtagcg
     4621 caagcgcctg agaaaaatct tgtgacgatc atgaacgatc aagggatcat ccgtccatgt
     4681 tcacaaaact tcccagaacg attcagagaa gccttcattc gtgaagccca agaatttgtc
     4741 tcatgtattc aagaaaaacg tcaaccagcg gttaatgcag aagatggatt gcaatcaact
     4801 aagatcgcgt tggcttgtca agaatcattc gatacgaaga gtttagtaca actaaaaaaa
     4861 taagcaggag tgtgagaata tgaccaaaca aattcaatta gccatcgcac caatcgcgtg
     4921 gaccaatgat gatatgcccg agcttggtag tgaaaatacc tttgaacaat gtatcagtga
     4981 aatggccctt gctgggttca ccggaacaga gattggcaac aaatatccaa aagatccaga
     5041 ggtgttggaa caatatttga atattcgcgg cttaaaggtt gccagtgcat ggttcagtgc
     5101 gtttctgaca actaaacctt ttgaagaaac tgccgaagcc ttcaaagcac atcgggattt
     5161 cttacatgcg atgggcgcta aagtcatcgt agtagctgag cagggtcata gtatccaagg
     5221 gattatgaca gcgccagtct ttgatgataa accagttttc actgatgaag agtggaaaaa
     5281 actggcggaa ggattagaaa aattagggga tttggcccac gaaaaggata tggagatcgt
     5341 ttaccatcat cacatgggta ccggcgttca aacgacggaa gaaatcgatc gtttaatgga
     5401 aatgactgat ccagacaaag ttaaattatt gttcgatacg ggacatttgg tcttttctgg
     5461 tgaaaatcca atcgcggttt acaaccgtca tcaagatcgt atcaaacaca tccatttcaa
     5521 agatattcgt caagaaacgg ctgaagaagt agaaaaggaa aaagacagct tcttaaacgg
     5581 cgtgaagaaa ggcgtcttca cggttcctgg tgatgggatg attgatttca ctccaatctg
     5641 gacagcaatc catgccagcg actatgaagg ctggatcgta gttgaagcag aacaggaccc
     5701 ggctaaagcc aatccatttg aatatgcagt caaagccaga agctacattc gagaggtcac
     5761 cggtctttaa tctattgaaa aggagctaga cgatgaatct tttttctata atcagtttcc
     5821 tagtgattgt catctgtgta tggttatttg cctattctag atccagaagt gtcgatactt
     5881 ctggatcaga agggttcttc atgggagggc ggagcttaac tgctttgcca atcgccggca
     5941 cgatcatcat gacgaacctg tccaccgaac aaatagtcgg acaaaatggt caaagttatc
     6001 atgcagggat ggaagtaatg gcctgggaag taactgctac gattgcgatc gtagctttgg
     6061 ctgtgatatt cttgccaaaa tattttaaat acggcatcaa tacaatctca gatttcatcg
     6121 agattcgtta tgatacggtt accaaacgaa ttatttcgat cttatttatt gtgacctata
     6181 tgacttcttt cttacccgtg gttctttatt ctggatcgct ggtgttcaat aagatcttcc
     6241 atattgatga gctgctgggc gtccagccga tcgtcgcgat cataattgta gcgatggcaa
     6301 ttggtattgt cggcatcctc tatctgctga tcggcggctt gtcgttaagt gccaatagtg
     6361 actccattta tggagtcggt ctgctggctt gtggtctgct ggtcccaatt ttaggcttaa
     6421 tgaagcttgg tgacggcagc tttgttggcg ggatcgaaca cgttgtggta aacacgccat
     6481 ggctgcttaa ctctgtggga tcgatcgact cggctgttgt tccatggccg acactattta
     6541 cagggatgct gtttaacaat ctgtatttct ggtgtaccaa tcaaatgatc gtccaaaaag
     6601 cattgtccgg caagaattta gctgaggcac aaaagggcgc tttcctagtc ggcgtcttca
     6661 aaatcttagg tgcgttattc cttgtctttc cagggatcgt cgctcgcaat atttttggcg
     6721 atagtttaat gtcaaatcca gataacgcgt atcctttttt agtcagtgaa gtattgccta
     6781 aaatgatctt tggtattttc gccgctgtta tctttggcgc gatcttatca tcatttgcgg
     6841 gagctttgaa cgcgacatcg accttgtttt cactggattt ttataaaccg cttatcaata
     6901 agaaagcaaa tgataagcaa atcgctcgtg ctggtaaaac agtaacggtc gttgtgggga
     6961 ttatctccat tatcgtagcg ccattcattt cttttgcacc ggctggattg tatcagttcg
     7021 ttcaagaatt caatggacta tacaacatgc cgctgctcgt gatcattctc tttgcctttt
     7081 attcaaaaaa agcaactgct tttggggcaa aaacgacgat tgccgtgcat atcgtcttgt
     7141 atgcactatc aaaaatgctt ttccaaaata tccatttcct ttatgttttg acggcattat
     7201 tcgttctgga cattatcatt ttgatggtgg tttccaaatt gaaacctgat ggagaatttg
     7261 tgttgaactc attcgataca aaggtcgata tcgagccttg gaaatacacc aaggtgactg
     7321 ctatagcatt ggtagtcatt gttattttaa cttatgtggc attttcacca attggctttg
     7381 caggctaaaa acaggagggt attatgcata aggatctatt gattatttgc gagaccggaa
     7441 tcagtgcgtc gctgctcgta tcaaaaatgc ttgaaacgat cagagccaaa aatctatcgt
     7501 atgagataga ttatgcaacg atccgaaacg ttgaagaaaa actgaattat aaagactatg
     7561 acgttttact tttaacgccg caggtcgcac gttctgagga aaaagtaaaa gaaatcatca
     7621 aaaaggaaaa atgtaccgct cagatcgttt atatcaaaca agaagatttt cgttatatga
     7681 atattgaaaa catcattgca agcatcgaag aagattaacg agaatctttg attattgagt
     7741 gtcgaggagg tggtatatga aaagatggca tgttcgcccc gatcacccca atatgaaggg
     7801 cggacttatt agaaataccc aatcttgtat ttattcaata gggtaagcgg acctcaatag
     7861 ccagctcatc cccaaaaagt cgaatggaga cacttaaatg agtgtaccta ttcgactttt
     7921 tgagactcta agtcaaatcg tgttgttctc attaaatcac tgaaaggaaa tgcttcggcg
     7981 tttccttttt tctttttgtt tttcttcgtg ttttgaaaat tacttactat ttactatacg
     8041 agtagtttca aataattttg acatcctaat cttcaacaac agaaaaaaat ctaaat
//
LOCUS       DAYYAR010000122         7966 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000122 DAYYAR010000000
VERSION     DAYYAR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.124"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..1649
                     /locus_tag="ACHAT0_002360"
     CDS             12..1649
                     /locus_tag="ACHAT0_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452273.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1794..2534)
                     /gene="truA"
                     /locus_tag="ACHAT0_002361"
     CDS             complement(1794..2534)
                     /gene="truA"
                     /locus_tag="ACHAT0_002361"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8452274.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2725..3360)
                     /locus_tag="ACHAT0_002362"
     CDS             complement(2725..3360)
                     /locus_tag="ACHAT0_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8452275.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3507..4727)
                     /gene="sstT"
                     /locus_tag="ACHAT0_002363"
     CDS             complement(3507..4727)
                     /gene="sstT"
                     /locus_tag="ACHAT0_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8452276.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5295..>7966
                     /locus_tag="ACHAT0_002364"
     CDS             5295..>7966
                     /locus_tag="ACHAT0_002364"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8452277.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2609 a         1492 c         1238 g         2627 t
ORIGIN      
        1 aggagaaaaa aatgaaaaaa aacaaaaacc aaataataga tatcattttt atgttatttc
       61 taagttttgc ttacgtcatc cctctattca tgtctatggg aatctatcat agtgtcaatc
      121 aggatactta ctttcattta tctcgaatca taggactaga taatgtatgg agttctccag
      181 taaacttcaa taacttcgat catcatggaa caatgatgaa tattttttat ccatggctta
      241 ctctttatcc agctttttta ttttataaaa tgatgggaaa tcttgtttta agttataata
      301 tctactattt ttttatcact tttttaacaa tggtcgtttc ttatttttct atgaaacaaa
      361 taaaaaacaa tcgttatatt tcgctgttat tttcgattat ttatacattt tccgcctatc
      421 gtgccattga catattccat cgtgcttcat tgggagaagc tgtcgcttta acatttttgc
      481 ctttgatact aatggggtgt tacgaaattt atattagaga ttaccaaaaa tggtactggt
      541 tgtctattgg catgacgcta gtagtgtata ctcatttact ttctgtcgct atggtttctg
      601 tatttattgg aggaacgttg tttctttctt tttatttttg ggaccaaaaa atcgctagac
      661 tcttaagtct tttaaaagct accgtcctca cctttttttt aagtgcggga ttcttaattc
      721 cttttattca acaatctaga gcacaagaat tgaaagtccc actgggaaaa gaattaagtg
      781 gtatggctcc tagtgatatg ctgacacaca ttttaaataa taactataac aactatacta
      841 ttggattatt cttatttctc ggattgattg gtgcttttat ttttataaaa aaattaactg
      901 ctgatgattt atttattttc tttcttggtg tatttgttct tttttgttca accaatttat
      961 ttccttggca actttttaac catacaccag ttaagagtct acaatttgtc tggcggttaa
     1021 atggtttttc ttctttattt atagcctata cgatgagtat agtgatctat tatatttttt
     1081 ctactaaaaa taattcatgg aaaataatgg tctttacgtt tcttgcacta atattgcatc
     1141 tctcaggagt aagcaattct atccatgcta acaaagattc attaacactt atagcacctg
     1201 aagacgctgt tgctatagct gaaaattaca atcatactga ttatgcaaac aaagagtcaa
     1261 tctatcatcc tgatatgatt aataatgatc aatatttact aggtaaccag gaaataaatc
     1321 caacaactca ttttatgagt aacaaactta caattgaatt agataattcg aataataaaa
     1381 atactgtctt gacgacacct atttatcgtt ataaagggca agtggcttct attaatggca
     1441 aactagtaca aacaaaatta tctaagtttg gcacaactga attaactatt cctccaggta
     1501 taaataaagt cgttattact tatcagtaca caaaactagc aattgcttca agatatttat
     1561 ctatcgttac tttgatttta tttttacttt atcgatttac tttttctaaa caaaaacaac
     1621 cgagaagaga agtacaacat tctcactaga tttttattta agaaagactt tttagctgta
     1681 cacataaaca ggctaaaaag tcttttttta tatttcttta atttgcttca tacattattt
     1741 aacatcgata tttatactgt gaaacatgcg tttcatccat tatcaaagta tttttatcga
     1801 taataaactt catccaaaaa cagaccttgt gcagggacgg tttctcctgc atgttgccgc
     1861 actttgcttt caaatatttc atcgattgtt tctatcggca tggcacctgt gccgatttct
     1921 agtaatgtcc ccatgataat ccgcaccatt ttatataaga atccgtctcc gacaaaagta
     1981 aaatgaagca tttctccctt tcgttcgata gataattcat caatcgtacg cactgtggat
     2041 ttttttgatt ttttcaatgc agaaaaacca atgaaatcat gtgtacccac taatttttcg
     2101 caagctgcat tcagcttgtc catatccaac tcttgaggga aataaaagct gtggtttcgc
     2161 tcaaatgctg aaggaatcac gttgttccaa acataatagc tgtatttttt gcccaccgca
     2221 ttatatcttg aatggaagcg ctcaggcatt tcttcgattc ttttgatgac aatatcccgc
     2281 ggaaggtaac gattcataaa gtcgatcatt tctgccagtc caatttctgt gtttgtttta
     2341 aaatttgcta cttgtcctcg cgcatgggtg ccagcatctg ttcttcctga tccaataatt
     2401 tctatctctt catttgtcat ctgatgcaat actttttcga tttttccttg aatggtttta
     2461 tcgtcatctc caagtctttg ccatcctgaa tatctttttc cgtcatattc gattgtcaac
     2521 ttgatgtttc tcatatttta gtggctcctc tgtcttccat ttggtcttct ctctaccttt
     2581 ttaagattgt agcacaaaat atagctcctt tcttctctaa aacagaagct atacaagaaa
     2641 actgtggaaa tatatatttc agaaaaacaa aaaacagcca ccggatgaag tatccgctga
     2701 ctgttcaatt tgacggctca agttttactg tttgagatgc aagatactca accaggcaat
     2761 gggcacataa ttaaagaaaa aactgattct ttggcaaaaa cctattttat tcagtaatgg
     2821 ccaagaattt atagaaggaa ttcttgccag tccataaatc gccgcaaaaa gcaaactgat
     2881 cacagacagt ccaaggtata agtggcttac gtccatttgc ccattttttc gatagatcaa
     2941 gaaaagaaga actggaaaca ataaaaaacc actgtaacct aaacctgatc cgatattatg
     3001 gacccatgtc gagacattcc acgaagattc atgggtattg atactgaaca aacctgtaaa
     3061 gatacaatca cctataccat agaacgcgat ggctgctgta actagggtag ctaacacacg
     3121 agatgtactt acaaatgcag catacaatgc aggcaatgac aggatgaaaa agatccctga
     3181 aaccacagac caaataagaa acgcttttct tacaggactg gaaacctctc caaatacact
     3241 gatcacgctc ttcatttgat ctaattcagg ataatacaat cctaatacgt acggagtcaa
     3301 aaaatcactc agcacgccta ataataaaaa gaaaaaacca aatttacgta aaaaatccat
     3361 gatacgcctc caataacaat tacctatgct tttaattgtt attataagcg actttatctt
     3421 gatttaacag aaacctctat ttacacgaac atctatataa ctcattaaaa actcgaatca
     3481 ttttaaaaat actaaatatc taacgttcaa cgttcactta atccatctaa acaatctttc
     3541 aatttgattt tttccccact tcttttacgc gcgcctagct ctcctattgc agtaaacaac
     3601 agatcacttg acgaatttaa ggcagtttcc attgaatcct ggataacacc gataataaat
     3661 ccaataccga cgatttgcat cgcgacatca ttagagatac caaacaaact acatgctaat
     3721 ggaatcaata acagtgaacc gcccgcaaca cctgaagctc cacaagcagc taaagcagac
     3781 aaaatacata aaaggacttt caaaacaaaa ggtgcttcaa tccctaaaga atgtacagca
     3841 gtcaacgtca tcaaggaaat ggtaatcgcg gcaccgccca tattgatcgt agcacctaac
     3901 gggatcgata tggaataaga ctcttttgtc aatttcaatc tttcagccaa catcatgttt
     3961 atcgggatat ttgcggcaga acttcgggta aagaaagcgg gaagtgcgct ttctttcaaa
     4021 caaaagaaaa tcaatggata gggattttct ttaataaaca aaaacgtgag gaatgggtac
     4081 acaactaatg cgacaaacag catcgtaccg atcaaaacaa gtaaaagctg cagatactct
     4141 gccaatccag ctattccagt cgtagcaatt gattgataaa ccaatcctaa aatccctatc
     4201 ggagcaaatg caataatcac ttgtacgact tgtgacaacg ctatagatat ctgatccacc
     4261 aactcttttg tcgattctga acgtgctcga aaagctagtc ccaacaatac agcccagaat
     4321 aagacgccaa ggtaattcgc atcaataaga gattgaacag gattttgtgt gacattcgtc
     4381 aacatagtag ataaaacact ttccaaactt tgcggtgctt tttcagatac tgtttcttgt
     4441 aaagtaatag gaacagtaaa aattgaactt acaataactg ctacaagtgc agaaagcaat
     4501 gttcccacta aatataagat caaaataggc ttcacgaatg tcttattccc aattttatgc
     4561 ttggcaattg acgccatcgt caagaaaaaa accagaatag gtgcgattgc cttcaagcaa
     4621 ctgacaaaca acgttcccaa tattgaaata aatgtccatg tggggaaaaa gataccaaac
     4681 aatattccga ctaaaatacc aacaacaata cgttgaacta acgacatatc caaaaacttc
     4741 tctaacaaat gaccaccctt tcacaatttt aacaatcatt gtatcatttt atgcaaagaa
     4801 actaaaggta taattttcta aaaattctaa caatacaaac aaccatttct tgctaatttt
     4861 gtactttttc tgtctacacg ttgttttttg agatattcta agttaggatg ttattaatgg
     4921 gcaacttatc aggaaacgtt ttcttatgtt tttgtccaat ttttttgtga ggtgtttatt
     4981 cttgaaaaaa ataaagaagg agagttatgg cttcctgaca atctttgtcg cttgtataat
     5041 gatggtttta cagcgtatat tgatttcagc agtcactatt cctacatatg caacaaaccc
     5101 tggtcgattc tacatctata catttttaca aacttccgtt gttattggtg caaatttgat
     5161 cccactttac atgggttacc aatatcaaaa aatcgcccgt ttaaaagttt tacattattt
     5221 aagtcgtttt gcctttatat tcctaatagc cacactagtt tgcaatatct tttttttacg
     5281 tacaagctgg cagcttgaat attcgtgatt attggcttat cctaacacct atcagtcaga
     5341 attactttac ttatgctgtt tcttgtactt tgcttttttg tagtattcct tatacagttg
     5401 gatttttaaa taaattatcg aaaacaacaa ttaaatatct actattattt ttaacaattg
     5461 gattagttgg ctgctctact ctatttaata aagacccgtt tgcattacag aatggacaga
     5521 gtattttatg gattttttat ttatttttaa tgggttatgg tttgaaagaa tggaactgga
     5581 aagaaaaggt ccgctataag cttcctcaag tatttatcag cttgattgtt ttattcggtt
     5641 tgattatttt gatgacacaa atttcactca tcatcagagg aaatacagat acagcgctga
     5701 ggttttctaa accatattct ctcttcgcaa tgtattatac tttctcttcg tttttatttt
     5761 tagaaatgct atctgataaa ttaggtataa aaggaagagg aaattttctt gctgtatttt
     5821 tgattgtcac tcaagttata tgtaatttcc ctttagtttc ttatcgcgta tctacattta
     5881 tgaaacgtga atttccaaac tcagggaagg catggctgat aaatatcggg cagtttttta
     5941 tttgctattt attagcagtg tccctcctag ttatcttttt attttatata caaaaaatcc
     6001 gtttcattca tcgtttcatc gaatacttcg cttttgattc gattgaacaa ttaactcagc
     6061 gttggaaaaa aagaattcat tggctttttg ttcataaaag ttatttttta gttgcagcat
     6121 ttttttactg ctttaccttc gtgcagattt tcgttttaga accaaaagaa ggttggaaag
     6181 aaactatcca ggttgcctta aaaatattta ctcagcgaca agcacccatg atcctgacaa
     6241 tcatcattat tatgggattt ttcttcttac tattgttgat ttccaatcgt ttttggtacg
     6301 ccttgactgc taccttgatc attaacttac tgctcactat ttcgacagta atcaaaatgg
     6361 aaatgcggga agagcctgtc tttccttcag atttaaaaat actgaccgga ttgtcagaac
     6421 tattatcgat ggttagccca gtcttattaa tcgtgggcgg attgatcctt ctcctattat
     6481 tgattactag tattattgta caacgtagac tacaaaaaca gtattccttg aagattcatt
     6541 ggaaacgtcg atttatcagt atcgctgtat tattaggatg tttttcgggt gtctttttca
     6601 ttaatcacaa aaattcgcct tctttcttgc tgtttaattt atttaaagta aacaagactt
     6661 tctttaacca gcaaggcgcc gtaaaagata acggaccagt cattcaattc ctaaacaata
     6721 tcgacattaa aatcatggaa gaacctgctg gctattcgaa agaaaaaatt aatggaataa
     6781 tgaaaaagta caacacaaag gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca
     6841 atcagacgat cgttctcaat ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc
     6901 tgacagtacc tacagaccct attccttaca tcaaaagcat tataacagaa ggaacaggag
     6961 gattgatgtt atccgtcggt tatggtggcg gaacagctaa catggaatgg gaaggactga
     7021 caggcttgag tatcagtaac ctgtctgctt cgttagttac accttatacc cagctagtgg
     7081 agcgtcagaa aataagtcca aatattaccg atctattcga tgaaaaaata gccatccatc
     7141 cttttacagc tgctctttat cgcagaaaac aagtgtttga taaatttggt tttgacaggt
     7201 tctattattt agacagtccg gataaattaa cttatacgga taaaatagaa caaagcccac
     7261 gcatatcgga tgattcagca tacaaagaaa ccttgaaagc tttacaaagc aatacagaaa
     7321 cgtcacaatt catccaatta tccactatgc aaaaccatat gccttatgat gattactata
     7381 gtgaattgaa ctacactgct gaaggaactg ctgtcattcc taatagaaga aaagaattac
     7441 aaacttatat gcaaggcctt cactataccg atacagcagt aaaaaaattc atccaagaaa
     7501 tcgacaaaat cgataaaccg attacctttg ttttttatgg tgaccattta cccgcaatct
     7561 atagtgggaa tgattcgaaa aaatatggat tagaacagca ccagacagat tatttcattt
     7621 ataacaacaa gtatagtcgt gaacaagcag ctacgttaaa taaagaagtt gttgctccta
     7681 ataactttgg tgctatggct cttgagcatg cgaatatcaa agtaactcct tactatgcgt
     7741 tgttgacaga agtatctaat catctacctg cattaacaat tgatcctaca gaaagcttgt
     7801 ctaatcgttt taatggaaaa caggtctttg ttacagagga tagtcaaatt atccaagaaa
     7861 aaaatttaac tgagaaacaa aaagaaattc tagaagatta tcgtctgata caatatgatc
     7921 ttgttgcagg taaccagtac agtgcaacat gggcgacaca aaaaat
//
LOCUS       DAYYAR010000123         7948 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000123 DAYYAR010000000
VERSION     DAYYAR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.125"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            430..660
                     /locus_tag="ACHAT0_002365"
     CDS             430..660
                     /locus_tag="ACHAT0_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452278.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            819..1652
                     /gene="terS"
                     /locus_tag="ACHAT0_002366"
     CDS             819..1652
                     /gene="terS"
                     /locus_tag="ACHAT0_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM8452279.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            1645..3060
                     /locus_tag="ACHAT0_002367"
     CDS             1645..3060
                     /locus_tag="ACHAT0_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8452280.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            3072..4601
                     /locus_tag="ACHAT0_002368"
     CDS             3072..4601
                     /locus_tag="ACHAT0_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8452281.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            4519..5571
                     /locus_tag="ACHAT0_002369"
     CDS             4519..5571
                     /locus_tag="ACHAT0_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8452282.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5573..5890
                     /locus_tag="ACHAT0_002370"
     CDS             5573..5890
                     /locus_tag="ACHAT0_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452283.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            6017..6640
                     /locus_tag="ACHAT0_002371"
     CDS             6017..6640
                     /locus_tag="ACHAT0_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8452284.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            6655..7545
                     /locus_tag="ACHAT0_002372"
     CDS             6655..7545
                     /locus_tag="ACHAT0_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM8452285.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7568..7783
                     /locus_tag="ACHAT0_002373"
     CDS             7568..7783
                     /locus_tag="ACHAT0_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452286.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
BASE COUNT         2917 a         1141 c         1626 g         2264 t
ORIGIN      
        1 attccattaa taatttcaat ttttgcgctt tgtcttaatg tatatatgat tggatttaaa
       61 aatgggcaaa ataaaaaata gtagcagcca agaataattt tatagtgtca ctgtggcgga
      121 aagggtagac gctaagcatg tgtgctaggt caatgcttcg gcaaccatgc aaggttcgat
      181 tccttgccag tgactttggc aaccgaggca tcggcggttt aaaaatatag gggtgcgcaa
      241 tttcgtacgc gttttgtgca tcgtgtaggt tgctattaca taactggtta ggttagattg
      301 agatttggga ttcggtacaa atgaatcgtc aaatgtctca agcacaggat cggaaacgtc
      361 cctgcctgtg cattacatat tagatcactc tttgagtggt ctttttcttt acataaagga
      421 ggctacataa tgagaaacta ctggtatgta tcgctaacta atgaatatcc tcgaaccatt
      481 gatgattgtt cagtgcgtgt tgtgcgttct gtacaaatca aagggaagta ctctattgtc
      541 gaaatgggga gagaagcaga accatgtgag attgatgcgt gcaagctcgt ttattgcggt
      601 catggtttct atgatgaacc aaacattcaa aataatatta acaagaattt gagggattag
      661 aatgcaagaa atggcttaaa aatctccaat caggacaaat caagttagac aaagtttccg
      721 atttaaagat attgattgaa gctgatttaa tgctaaaaga tattgaaaat tagaaaacaa
      781 aactcaacct aaacagaatg tgaggtggtg tgtattgaat ggcaagacaa cgtgatccaa
      841 gacgtgatga agccaaaaga atttggttag aatccaacgg agaaaagcag ttaaaggaaa
      901 ttgcatctga attaaatgtt tcagattctc aggttagaaa atggaaatcg caagacaaat
      961 ggagcgctga attgattagt aacgttacca atggcaaaag taacgttact aatcaaggtg
     1021 gcgctcctat tggtaatcaa aatgctaaag gtaacaaagg aaatagccga gcttctccgc
     1081 cagtgggtaa taaaaacgct ttgaaaacag gtgagtatga aacaatattt tttgacacgt
     1141 taaccgataa tgagaaggac atctattcta gtttgaatga tgatccttct tttgctttgt
     1201 ccgaagaaat acgacttctt aaaataagac aacttcgaat gatgaaaaga atccaacaag
     1261 ctgaagctgg actaaatgat gaagaagtcg aacgattgca gcagctaaga aagattaaaa
     1321 ctccgattga aaaaaatggt aaaaagctag aaatcaagcg tgaggttatg caagatgtgc
     1381 agattagcag aaaaaaacat cgcaaaattg atgatattct ttcaattgaa gattcattga
     1441 ctcggattag caaccagtta gctaaagcca tcaagcaaat gaatgaactt tatatgaatg
     1501 aatacagaac tgatttaatt aaagctcaga ctgataagat ccaagctgag acaaatgaaa
     1561 ttggcggaaa taattcaggt gaagaaatag aagaatggaa acaggcagtt ttaaatgccg
     1621 caaacaaacg ggcggtgaaa gaaaatgaat aatgaattta ttccttttgc tgatattggt
     1681 tctgccattg attattacta tgataaacca gtagcttttt gccaagatat tttgcatttg
     1741 aatcctgatg aatggcaaga aaatgtttta aatgatttag ctgaattttc aaaggtttct
     1801 gttcgttctg gtcaaggagt tggaaaaaca gcattagaag caggagcaat actttggttc
     1861 ttaacgtgtc gaccctacgc taaagtaata gcaacagctc cgacaatgaa gcaactttac
     1921 gatgtacttt gggcagaggt agctaaatgg ttaaatgata gcttgatcaa aaacttactg
     1981 aagtggacaa agaccaaaat ttatatggtt ggtgattcag agcgttggtt tgctacggct
     2041 agaacagcga ctaaaccaga aagtatgcaa ggttttcacg aggaccatat gttgattgtg
     2101 gtagatgaag cttctggtgt gtctgatcca attatggaag ctattcttgg tacgctatca
     2161 ggttttgata ataagctgtt gatgtgtgga aaccccaata atattgaagg tgtcttttat
     2221 gattcccata attcagaccg tgataaatac agagttcata aagtatcaag ctatgatagt
     2281 aaacgtacaa acaaagacaa tatagaaatg attcttaaaa aatatggaaa agaaagtgat
     2341 gttgctcgtg tccgtatttt tggagaattt ccgaaaggtg cgttggattc atttatcagt
     2401 cttgaaacag ttgaattggc tacagaaaaa caaattagtg attctttagt caataaaaca
     2461 aaggttgctc atattggtgt cgacgtagct cgatatggtg atgattctac gattctcttt
     2521 cctagaattg ctaccagggc attggagtat gagaagtatt caaaacgtag caccatggaa
     2581 acaacaggat atgtcatcaa catggccaag aatctaatga gtcaatatcc gagtattgat
     2641 aaagtgatga ttaaagtcga tgacactggt gtcggaggtg gtgtaaccga ccgcctagaa
     2701 gaacttatag aagacaaaca ttatcctttt gaggtgtttg gagtgaataa cggttcaaca
     2761 tcagaagacg atttttacga taatttaggt actcaactat ggggaaacat caaggaaatg
     2821 ttagaagaaa atatgacagc aaatcttaac ggagaacagc ctgttattga attgccttct
     2881 gatagttcgt taatcaaaga attaagtact cgcaaattca aaatgacaag tagaagtcgt
     2941 atacgtttag aaagtaaaga tgatatgaaa aagcgaaata ttggtagtcc cgatattgct
     3001 gacgcactgg ctttagcgtt ttatgagcca ccaagtcact atcaatttat tcaattttag
     3061 gaggtgagct tttgattagt acagtattat ctttagagag atataagaag ctacgtgtta
     3121 aatatgcaac acaaattgaa gatggaatgt ttgatccgaa tggttttata gaagatatga
     3181 aaccattttt tgctgatcgc gaaagaaaat atctagctta tactagcgaa aagaatgaaa
     3241 tagataatag accaaagcct aacactgata ttgtaaaagt taataataaa cttcatgctg
     3301 gtatgtattc aattgtcgta gaccaagcag tcaatcattt tactggtata cctatcaaat
     3361 gggattacga tgtatcggaa caaaaaagaa cactcattca aagattaaaa gataaattct
     3421 taaaaaatga tatagaactt ccgacagttc cagaagcttt taacaaatta acaagcaatc
     3481 ttgattccat gagattcgca atgcttgatt ctgaaactgc aacttttcaa ggagcttgtg
     3541 gagtagcttt ccgattgcta gagcctgtgg aagaagatga tggttggaag ttaagagcaa
     3601 gtaatattga accttggaga gcagaaagat atggaaatgc cggaatctat atcaaagaaa
     3661 agtatgactc ttaccagaaa aaattttttc aagaaatgaa agttatgaca agaaataaaa
     3721 ttctgacata tgcttgctat ggtgatttga attttgttac aagcggaacg ttcaaaaaaa
     3781 ttgatgagac cgataaccct ttggggacga ttaccttggc agaatttaaa aataatacga
     3841 atcgttattg tgattttgaa gtagctgagg aaattggaga tgcaattgat cgtgctttgt
     3901 ctgatcaaca aaacgaaatc gaacaattta agcttgcgta catgcttgtt actggcacaa
     3961 caatgagcaa aggcactgct aaagaaatga tgaatcagct agggattatc aacttaaaag
     4021 cccctactgc aaaagctgaa tatgtcacaa aaaatttagc aaaagatttc aatgaatatc
     4081 atatggatct attgaaaaaa cagttttaca ccatttgtaa agcaatcgat tttaacgatg
     4141 aggtgtttaa atcaaacagt tctggagaag ctcgcaagtg gcaaatcatt agcctagaag
     4201 cgaaaacaaa tactaaagag caatatttta gagaaggatt gaaagaatgc gcagaaacaa
     4261 ttgctgcttt tcttaagttt catgacaaag tagaaattga gcctgaaaaa attattttta
     4321 ctttctctag atctttgcca actgatttaa gttatttagc tgaagcttta cctaaacttg
     4381 caccatacgt atccaaacga actattcaga gccaaattcc atttgtaaca gatgttgatt
     4441 atgaaaatga aatgatggaa ttggaaagtg gaagtgctta tccagatagt gaatacaatt
     4501 ttggcggagg tggcaatagt gacgatagaa acgaagtatt ggaccaaacg tcgagaactg
     4561 gaggatcaag caaggctcaa acaagaaaat cagacactta aaaaattaac tagtgtattt
     4621 cctgaagcac tgaaagagat acaagcaaaa ctattatcac aagctgactt acacaatatc
     4681 acttatccag aaatgatgga gttttacagt acaagtaatc agaaaaaata tcgtgaatat
     4741 gtggaaaaaa attataagtc attaaaaatg tatgatgcaa aatacaaaga gtttatcgat
     4801 gaattttttc caccatttga ctatgcaaaa gtcaatcgct tattacaaat acgatcagat
     4861 gtatttaaaa ttcttgcaga atatgcgatg gatgcagatg tgaatcaata tttttctgat
     4921 cgcttagagg aaattcttca aagaacatat tcttctaatg ctaatgtttt tgttcaactt
     4981 ttaaacgttg atataccaaa ttatttacca gaaaatgaaa ttaaacatta tttgaactat
     5041 ccatggtcag gaaagacatt ttcaagaaga ctttggggaa atatttcatc gcttgaacag
     5101 aaactctcta atgctattgt aaaaagtgtt gctagtggag aaggcgttat acacgcatta
     5161 aacaccatga gattagactc agaaatttgt gacatgttta agttagaaga atcaaagtat
     5221 aacaaagcga tagaaaatct cgttcgaacg gagtatgcaa aatttgcaca agatggtatt
     5281 gaaaaatcat atttagaaac aggtattgag gaatacaacg tattgactgc aaaagatgag
     5341 agagtttgcc ggatttgtgg aggaaaggca agtaagaatc cctataaact gaaagatgct
     5401 gtcataggtg aaaatcgagc acctttccat agtcgttgta gatgtacgga tgttcctaat
     5461 ttaccaaaat taggaaagga tattgatgaa gaatatgacc gtttatttgg cgatttatta
     5521 gatgagtttg cacatgattc ttttggaatt aatttgaaac ggaggaagta gaatgaaaga
     5581 tttttttgaa gcagtactaa caattaatgt aaatgctgat attgcagaag cctacaaaac
     5641 agctattgag tctgagaacc atcctaatgg cttgagagac cattggaatg gcaattatgc
     5701 ctacgtggtt attggcgatc aaactgttaa ttatcaagat aatactccag ttgataagaa
     5761 taccgttaat ttaacgattc aattattatc tcattcatta ccaaatttaa aagaaacagt
     5821 tgattggtat gaaaagatgg gatgtattgt tgttagaact gactacaaag aaggaaagtc
     5881 tagtaattag gcttttttat tttgtccgaa atgacgttaa actagcgcaa tactgggctt
     5941 ggttgaatgg tggggcgcaa ctattaaaac tcaaagcaat gcggggcgtg aaaacgaatc
     6001 gtggggcgaa aggagaatga gcatgaaaca caaatcatta atgccaatga atttgcaata
     6061 ctttgccgaa ggtgacgatc aaaagttttc ttttgatgac ttcaaatctt ttgtggaatc
     6121 caatgaagaa gctcaaaaat ttattcagtc acaatctcaa agtgttgccg acaaacaatt
     6181 ggaagcttgg aaacaaaata acttagataa aattaaacag gataccatca aggaatatga
     6241 ggaatctaag aaaaacaagt cacctgagca aattcaactg gaaaaactac aagctgaatt
     6301 tgaagcagaa aaagcgttgc gtgtgacaag tgataataaa gcatttgttg cagaacaaat
     6361 tgctggatta gaactagatg gagagttaaa agagtctatt tctcaattta tgctaaataa
     6421 tcttgttagt tcggatacag atttcactaa gaatgctgtt gaaggtttta caagtgtttt
     6481 gaatgcaatt aatgagaaac atgcagatgc attaaaagaa ctacaaatga agtctgcatt
     6541 tggtggaact caacaatcga ataaccaggt tcagcagaac aatgaaacat taacaaatcc
     6601 agaagaacaa ttaggacaaa ttcttcaaca atttaactag gagagtgaaa aattatgaaa
     6661 aaaacatctt taaataattt agagtatttg gatatttcac cagcgattaa tgctatgcaa
     6721 gtaccaaata cacctttttt aagctattta cttggtgctg gaaaaacaga gccagcaaac
     6781 tcgacagaaa ttaaatggcg tgaatatgat atcaacaacg atgattcttc tgaaaaactt
     6841 gagggcggag aatatccaga tgctgaatca ggtcgaactt ggtttaacaa ctatactgaa
     6901 atttttagaa aatcaacctc tgtatctggt acattagatg ctattaatgt gaatggagtc
     6961 ggaaatgaat taactaatca agtagcacta cgtggtatgg aaatgaaaat tgatttgaac
     7021 cgaaaattga ttactggtgt aaaagctgat gaaaatggtt ctaaaggtcg tcgaatgaat
     7081 gggattttga acttgatcaa ttcagcaaat aaggcagaaa cagccactgc gggtgcagta
     7141 acaagaaaag atatcgatac tttatttaaa ttgatgtatg aaaaaggtta tatgggagaa
     7201 aaactatgct tgatttctcc agatatgcag gagttaatga ctgatgagtt agatggaaaa
     7261 tcaacaaaaa ttgttcagtt cggggaaaga gtaacttttg gattgcaaat tggaaatatc
     7321 gtgtctaatt acggtacagg tattgctcta ctagaaccat cattgccaaa aggaacaatt
     7381 gccgcaatcg atactaatta tgtgaaacta cgtccattac gtgaatggag agcagaagaa
     7441 cttgcaaaaa caacggattc tagacgtatc ggtcttgtag gtgaatactc tcttgaatac
     7501 aatgcttcaa actctggggc aattttaaat ttaaagtctg aataaaaggg agttagtact
     7561 cccttttttg ataggaggaa ttacagtggt aaaaaaagat gaaactaaaa aagatgaagt
     7621 cgtgaaatat agagtaggta aaactaaaaa ttttgttggg tttgttcatc ctaaaactcg
     7681 tagatttatc acagcagatt caaataacga atttatcatt tctatagatg ataaagaagc
     7741 aattgcaatt ttggaagatg caattgatgt taatgaaatt taggaagtga tctgatggat
     7801 gaatcgctaa aaacggaaat cattgagtct acaaaagaag attttccaga tttgagtgaa
     7861 gaacgcataa ctaatttatt agaaataatt ttgctagaaa ttgaatcata caacacttgt
     7921 aaaaatgata tttcatggga aaagttaa
//
LOCUS       DAYYAR010000124         7930 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000124 DAYYAR010000000
VERSION     DAYYAR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.126"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..1704
                     /locus_tag="ACHAT0_002374"
     CDS             166..1704
                     /locus_tag="ACHAT0_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8452287.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1701..2666
                     /locus_tag="ACHAT0_002375"
     CDS             1701..2666
                     /locus_tag="ACHAT0_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8452288.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2653..3975
                     /locus_tag="ACHAT0_002376"
     CDS             2653..3975
                     /locus_tag="ACHAT0_002376"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8452289.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4038..4142
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4216..4959
                     /locus_tag="ACHAT0_002377"
     CDS             4216..4959
                     /locus_tag="ACHAT0_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8452290.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            4974..5966
                     /locus_tag="ACHAT0_002378"
     CDS             4974..5966
                     /locus_tag="ACHAT0_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8452291.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            5978..6715
                     /locus_tag="ACHAT0_002379"
     CDS             5978..6715
                     /locus_tag="ACHAT0_002379"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8452292.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6705..7427
                     /locus_tag="ACHAT0_002380"
     CDS             6705..7427
                     /locus_tag="ACHAT0_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8452293.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7511..>7930)
                     /locus_tag="ACHAT0_002381"
     CDS             complement(7511..>7930)
                     /locus_tag="ACHAT0_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8452294.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2532 a         1382 c         1732 g         2284 t
ORIGIN      
        1 ccaaaaagtt gatccgactt tgttgcttgg attctttttt tgtcagcata tttagaggta
       61 tttttttctt ttttttgttc gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa
      121 ggaaagaaat gtttatactt aaagaataac agaaaggaag gtcgcatgaa taattttata
      181 caaacattaa gtgaaagaaa aggcgagttg ctggaagctg catttcaaca tctgtccatt
      241 tctctcattt ctttgctgat tgcagcactg attgctattc ctttagcgat ttgggcagcc
      301 aatcataaaa aaatggcaga agtcttattg cagatcacgg gtgtgctcca aacgattcct
      361 tcacttgcct tattaggttt acttatccca tttgtaggga tcggaactgt tccagcattg
      421 attgcacttg ttatttacgc tttgcttccg attttccaaa acacgtatat tggtctagct
      481 gaaattgacc cgtctattga agaagcagcg gtagcatttg gtatgtccag aatgagacgc
      541 ttgctgaaag tagagcttcc catcgcatta cctgtcatta tctcaggtat ccgaactgcc
      601 ttagttttaa tcatcggtac ggctactttg gcagcattga tcggagcagg tggattaggg
      661 acatttattt tgttaggtat tgaccgaaat acacctgttt tgacattgat tggtgcgatc
      721 agttctgcct tgcttgcaat tatattcagc ggactgattc gctggttgca gcataagaaa
      781 cctcgatatt ccatcattac tttagcagca gtggtgtgtt tatttggcgg cgtttcttta
      841 tatcagtctg ccttgtttaa aaatgaaacc atcacgattg ctggaaaatt aggagcggaa
      901 cctgaaatcc tgatccaaat gtataaagaa ttgattgaag acaacacaaa aacagaagtt
      961 gaattaaaac cgaattttgg caagacgagt tttttgttca atgcactgga tcaaggtcaa
     1021 atcgatatct atcctgagtt cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa
     1081 gcaatgaaaa gtgctgattt gacaaaagaa aaaacatatg acgaagcaga agaattattg
     1141 aataagcaat tcgatatgac attattaaaa ccaatggctt accaaaatac gtatgccttg
     1201 gcagtcaaga gatcttttgc gaacgaacat caacttaaaa agatttccga tttggcagct
     1261 atctctgatc agatcaaagc gggattcaca ctggaattta ttgatcgttc agatggatat
     1321 aaaggaatcc aagaagcata tggcttgaat ttttcaagtg tacaaagcat ggagccaagt
     1381 ttgcgttacc aagcaatcaa taatggagat gtcaatattg tggatgccta ttccacagat
     1441 agccagctca aggagtacga tctagtgact ctagaagatg atcgagggtt attcccagct
     1501 tatcaaggcg caccgctgat gaaaaaagat tttgctaaag aacatcctga aatcgttaaa
     1561 gcactgaata aattaagcgg aaaaataaca gaggatcaaa tgattgatat gaattaccaa
     1621 gtcaatgtcg aaaagaaaaa gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga
     1681 ttagtaaagg aggaagatca gtgactaaat taatcgagtt caaacatgtg caaaaaaagt
     1741 atgatggaaa gtatgtcatc gatgacctaa atctggcaat caaaaaagga gaaatctttg
     1801 ttttggttgg tccttccggt agtggaaaaa caactacttt gaaaatgatc aatggattat
     1861 ctaaaccatc agcaggagac atctacttca aaggaaaatc gttaaatgaa tataatctgc
     1921 agaaaatgcg gtggaatatg ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga
     1981 cagtcaagca aaatattgaa gtcatccctg aaatgttagg ttgggaaaag cagaaaagag
     2041 cagaccgtgt agatgaactt cttcaaaaag ttggcttatc tcctgatatt tatcgagatc
     2101 gtatgcctag agagttgtct ggaggagaac agcaacggat cgggatcatc cgagctattg
     2161 ctgcaagccc agatgtgatt ttgatggacg agccatttag cgcattagat ccgatttcaa
     2221 gaaactcatt acaagaattg gttttgtcct tacacgaaga attgggcacg accatcgttt
     2281 tcgttacgca caatatggaa gaagcaatca agctaggtga tcgtattgca tttatgaagg
     2341 atggagaaat tatccaatgt gacacaccag agcagttatt gatgaatcct aaaaacgact
     2401 acgttcggca tttctttgat gaaccgaaac agacgaaaga gtggcgagta gaagatttag
     2461 tcgtcaatgg atatttctta aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg
     2521 atacgcctat gaaagaagta tattcgcttc tttcggtgta tccaagcatt gtgatagtag
     2581 aaaagcaaag aagtattggg tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag
     2641 aggaggaagg aaatgaaaac atatgatgca atcatcatcg gttcaggtgt gagcggctta
     2701 agtgctgctt atggattgaa agaagcaggt aaaacagtac tagtggtcga agaagatcta
     2761 tggggcggga cttgtcctaa ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta
     2821 gaggcacgta atcgggtaaa acaattgagt ggaaaaggtt ttaacgagat cccaactgcc
     2881 aactgggaag aacttcaaaa attcaaacga acatttacgg atccagtccc agaaagccga
     2941 aaaaaacaat tagcagaagc tgaaatcgat catctttcag gaaccgctcg ttttttggat
     3001 gattcatcta ttgaagtcaa cgaagaagtc ttccatgcgg attacttagt tttggcaacc
     3061 gggcaaagac ctacgatctt accagtggag ggaaaggaat acttgaaaac tagtgcagac
     3121 tttttatcat tgcctgtact gcctaaagaa atcattttta tcggtggtgg ttatattgct
     3181 tttgagctag ctacgattgc caatgctgca gggagcaaag taaccattgt ccatcataat
     3241 caacggccat taaaagaatt tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa
     3301 gcatctggca ttcagtttgc ttttggtgtt gaaacccaaa agatcatttc agaaggtact
     3361 cgctatcgtt tagtcggaaa agagacggaa ttagtagcag atatgatttt ttgtgcgact
     3421 gggagacagc cgaatacaga atctttagca cttgagcaag cgaatatcgt ctttgataaa
     3481 cacggaattg cagtcaatga ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga
     3541 gatattgttt caaggaaaac cccaaaattg acaccagtcg ccacttttga aggaaattat
     3601 gtagctaagc ggataacgga tgcaacaagc gaacctatca aatatccgat tatacctacg
     3661 atcgtctacg ccagtcctaa acttgctgaa gtaggagtta caaaaagtca cgcctcttcg
     3721 tcagatcaag tggtagagat ggatcttacc agttggttca cctatcatcg tgtcaatgaa
     3781 ccagttgcta aggctgaatt gacctttgac cagcaaaatt atttgattgg tgctgcagtg
     3841 attagcgagc aagcagacga attgatcgat gatttgacac tcgtcatcaa tcaaaaattg
     3901 actaaaaaag agttggatag ctatatcatg gggtatccga cattggcaag tgatctctcc
     3961 tatttattga aataatcagc atgataacta aagcgcttgt tgctttttcc ttcttgtgct
     4021 acactttaaa aaatgaatac ctgtttctgg ggatgtcgta gacgacatga gagtaagttg
     4081 tgaacttaga cccattgaac ctgtcagtta atactggtga agggagaaac atagcagacg
     4141 cttgaagcgg gttactatgt cgtgcccatt tcaagcgttt tttgttattc atattagaag
     4201 aaaggaggta acaaaatgaa gaaaaatctt tatccagcag tcagtatggc attcatttta
     4261 attatttggc agattgttgt catagtttgg aaaatacctg cctttatttt acccggacct
     4321 gctgctgtat tttctgcatt agcggcagat gcacaagtat tactttccca ttctgtcacg
     4381 acattaaatg aagcagtgct tggtttggta atagctagcg ttctttcttt tattactgcc
     4441 ttggcaatgg attattggaa gtttttagaa atgtcttttt atccgttact agtgatttca
     4501 caaatgttgc caatcatggt gttagggcca ttacttaccc tatggtttgg tttcggtatg
     4561 cttccaaaag tgatccttgt tgtcttgata agctattttc cgatcgtagt ttctttttcg
     4621 gatacattga ggaaagtatc aaaagagcaa atcatctttt tgaaaacgat gggggcagat
     4681 acagggaaga tttatcgaat atataaaata ccagcgggtt tatccggctt cttttccggt
     4741 ttaaaagtgg ctgctactta ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct
     4801 caagcaggct tagggtatta catgatacgc gtaaaaaaca gctaccagat cgataaagtt
     4861 tttgctgcga tcatttgtgt cattctattg agtttattgc tgaatggcgc ttgttcatta
     4921 ttgaaaaaag ggtattatcg tatgctttat attttttaag agaaggggat caaatgaaaa
     4981 aattatggct tttattaccg ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa
     5041 caagtagcaa acaagaaata aaggatttga aaaaagtgac acttgttttg gattatgtac
     5101 cgaatactaa tcatactggc atctacctag caaaagaaaa aggctattat aaagaggctg
     5161 gtttaaatgt ccaaatcatc gaaccaggag ataattcgac aagtattggt ctagtaggtg
     5221 cggacaaagc acaatttggt gtaagttatc aagaagatgt cacttatgct cacgcagatg
     5281 gacagaatat accagtcaaa gcaattgcta cagtgatcaa acataatact tctggattcg
     5341 ccaccctatc agacagtaac atccattcac caaaagattt tgaaggaaaa acatatgcag
     5401 gttggcaaag tccaagtgaa gaagctgtct taaaagctgt gatggaaaaa gacggcgggg
     5461 acttttcaaa actgacaatg gtaggaagca atggagaagg gccagaaagc ttaggaaaat
     5521 cctcagatat ccaatggtac tttgaaggtt gggatatgat caaagcaaaa gaagcgggta
     5581 ttgaactaaa ctatatccca ttgaaagaat tagatgaacg gttagattac tatactccgg
     5641 taattattac gaatgatcaa ctgatcaaaa gtgatccaga attggtacag tcttttatgg
     5701 atgccacgaa aaaaggctat caggaagcta ttaaggatcc aaatgacagt gctaaactgt
     5761 tgcaaaaata tgcaaaagaa aatgatcgga cgtttttaga agaatcacaa gcattcttgt
     5821 caaaaaatta tacggatgat cctgagaatt ggggattgat ggaagaaaaa gtttggcgca
     5881 attatacagc ttttatgcag gaaaacggat tgatcaagca agatgtacct tcgcaagaac
     5941 tattcactaa tcagtttata aaataggagt gatagatatg tcaatccaaa tcagccactt
     6001 gaatgtagtc attgaaaacc tgaaaatatt agaagacatc catttcacta tccctaaaga
     6061 tacctttaca tcgattgtcg cgccaagcgg agccggtaag tcaacattgc tgaaaacttt
     6121 aacaggagtc cagcctatga cttctggaac gatcaaagta gacgatcaaa aagtaactgg
     6181 tctccataca gcatttagtt atatgccgca agaagatatg ttacttcctt ggctgagcgt
     6241 ctatcaaaac gttacgttat atcaaaaaat caatcatcta acaatcaatg aagaacaagt
     6301 aagggattat ctggatatct ttgggttaaa cggctatgaa cattttttgc cggaacaatt
     6361 atctggcggg atgaagcagc gaacggcctt gttacgaaca atcatgaatc cttcttctta
     6421 cttgcttctt gatgaaccat ttggagcgtt agatgctatg accagagggc agatgcaaga
     6481 ctggatgttg aagctgccta aaaaagcaaa gcggactact ttacttgtga cgcatgatat
     6541 cgaagaagca atttatttat cagacaggat tcttgtgctt tccgcccgtc cggcacatgt
     6601 gattgctgag attcaagtgc ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc
     6661 agagttgaag caaacgattt atcagttatt ggcaggtgaa ttagatgtta agtgatgctc
     6721 attgtcattt agatgggagt cagagtttag cactccttca gcaggaacac acgattttga
     6781 cgatcatcaa ttgtgattcg ccagaagaat ggaaggaaaa cagacagctt gcagcaagta
     6841 agactcaagc cttaagttac ggtatccatc cttggaaagc agatagctat acgtttgaac
     6901 aagttgagcc ttttttgaaa aaagcaagga tcatcggtga aatagggtta gataatattt
     6961 ggacgaatgt accgatgact acacaaaaaa aagtatttga gcgacagctt gcttttgcag
     7021 ccataaatga gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt
     7081 atatccaagc ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac
     7141 aagactatat tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag
     7201 cagtctggca actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac
     7261 tacatgcagc agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc
     7321 ttgaagaaca gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa
     7381 aaaatactta tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga
     7441 cacaactagt gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa
     7501 taaatgaatc ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac
     7561 cataacgtga agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac
     7621 catttaagta agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt
     7681 aagaaccgct tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag
     7741 tagcagcagt ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt
     7801 tttccgtttg aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa
     7861 cggctgcttg aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt
     7921 gtcctgagta
//
LOCUS       DAYYAR010000125         7884 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000125 DAYYAR010000000
VERSION     DAYYAR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.127"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(318..1016)
                     /locus_tag="ACHAT0_002382"
                     /pseudo
     CDS             complement(318..1016)
                     /locus_tag="ACHAT0_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            1095..1271
                     /locus_tag="ACHAT0_002383"
                     /pseudo
     CDS             1095..1271
                     /locus_tag="ACHAT0_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1447..2016)
                     /locus_tag="ACHAT0_002384"
     CDS             complement(1447..2016)
                     /locus_tag="ACHAT0_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8452295.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2090..2413)
                     /locus_tag="ACHAT0_002385"
     CDS             complement(2090..2413)
                     /locus_tag="ACHAT0_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452296.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2567..3379)
                     /locus_tag="ACHAT0_002386"
     CDS             complement(2567..3379)
                     /locus_tag="ACHAT0_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452297.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3706..4581
                     /locus_tag="ACHAT0_002387"
     CDS             3706..4581
                     /locus_tag="ACHAT0_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8452298.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4595..4918
                     /locus_tag="ACHAT0_002388"
     CDS             4595..4918
                     /locus_tag="ACHAT0_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452299.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4915..5667
                     /locus_tag="ACHAT0_002389"
     CDS             4915..5667
                     /locus_tag="ACHAT0_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8452300.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVCLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5657..7828
                     /locus_tag="ACHAT0_002390"
                     /pseudo
     CDS             5657..7828
                     /locus_tag="ACHAT0_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2361 a         1544 c         1409 g         2570 t
ORIGIN      
        1 ggatttaagt aagttctaac taatcacata taaaaataaa aaaacatttg tattttatca
       61 aagttttatt atacttgtca atttgtgtac atcctaaaat ataagctatt tgtttacttc
      121 tgtagaatat atactttgtt gttagataag ttgcacatgt ttatagaatc taaataataa
      181 atttttatgt accgcccctc aaagtgaact gaaccccaaa agttgaacta tttaatggac
      241 tgtttccgat attctactgg agatagtcca ttaaatagtt caacttttgg ggttcagttc
      301 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
      361 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      421 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      481 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      541 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      601 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      661 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      721 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      781 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      841 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      901 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      961 ttcttttcaa aagtttcttt atcatgtgta tgttgtaact cgatttgcac aggacttttg
     1021 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
     1081 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
     1141 aaggtttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
     1201 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
     1261 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1321 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1381 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1441 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1501 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1561 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1621 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1681 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1741 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1801 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttc
     1861 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1921 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1981 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     2041 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     2101 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     2161 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcgatg atcactagat
     2221 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     2281 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2341 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2401 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2461 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2521 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagtcat tcttggtaaa
     2581 gtacttttgt cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2641 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2701 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2761 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2821 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2881 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2941 tcaaaaagca aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     3001 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     3061 gatcgtactt ctgctcgtaa ggttcattca atagcagatt tttcagtaaa caaaacgggg
     3121 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     3181 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     3241 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3301 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3361 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3421 aaatgggttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3481 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3541 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3601 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3661 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3721 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3781 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3841 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3901 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgtta
     3961 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     4021 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     4081 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     4141 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     4201 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     4261 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4321 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4381 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4441 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4501 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4561 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4621 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4681 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4741 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4801 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4861 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4921 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4981 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     5041 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     5101 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     5161 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     5221 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     5281 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5341 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5401 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5461 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5521 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggtttgt
     5581 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5641 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5701 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5761 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5821 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5881 gaagacattt tccatattat ggatcttaca gtttttttcg tcagtcacag aggaattctt
     5941 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     6001 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     6061 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     6121 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     6181 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     6241 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6301 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6361 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6421 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6481 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6541 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6601 agacaaagaa aacattacat tgaccctaaa aaaacaagtt ttcttacctt atccttatgg
     6661 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6721 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6781 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6841 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6901 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6961 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccaaaaga
     7021 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     7081 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     7141 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     7201 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     7261 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7321 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7381 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7441 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7501 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7561 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7621 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7681 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7741 taaaaaaacc tatttagaaa taacgcatat aataagaaaa atggtataca aaaaaattct
     7801 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7861 gccgagaacc agctgattga gtat
//
LOCUS       DAYYAR010000126         7286 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000126 DAYYAR010000000
VERSION     DAYYAR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.128"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..575
                     /locus_tag="ACHAT0_002391"
     CDS             <1..575
                     /locus_tag="ACHAT0_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8452301.1"
                     /translation="LLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEP
                     KYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLI
                     PLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAA
                     AGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            587..1507
                     /locus_tag="ACHAT0_002392"
     CDS             587..1507
                     /locus_tag="ACHAT0_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8452302.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1548..3005
                     /locus_tag="ACHAT0_002393"
     CDS             1548..3005
                     /locus_tag="ACHAT0_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8452303.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3350..3964
                     /locus_tag="ACHAT0_002394"
     CDS             3350..3964
                     /locus_tag="ACHAT0_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8452304.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            3957..5681
                     /locus_tag="ACHAT0_002395"
     CDS             3957..5681
                     /locus_tag="ACHAT0_002395"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8452305.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5678..7129
                     /locus_tag="ACHAT0_002396"
     CDS             5678..7129
                     /locus_tag="ACHAT0_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8452306.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2490 a         1215 c         1456 g         2125 t
ORIGIN      
        1 ccttactacc ttattttcta tcttgggtca ttatcagtta ctttgtttac gcttttttaa
       61 gtcctgacaa aggaatattc aatcagtgga tcactggaca tggaggagaa gctgtcaatt
      121 ggtataatga accgaaatat tggccgttta tcttggtgtt cattggtaca tggaaaggga
      181 ttggttataa cagcattatt tactttgcat ctgttatggg aatcgatccg acttattatg
      241 aagctgctat ggttgatgga gctagcaaat ggcagcagat caaacatgtg acgataccac
      301 aacttattcc actaatgacg attttaacga ttttagccgt aggaaacatt ttccgagctg
      361 attttggcct gttttataac attcctagga attccggtgc attatatgat gtgactcaag
      421 ttttggatac gtatatctac aatggcctga catccacagg agattttggt atgacggctg
      481 ctgcaggact ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg
      541 cccgacgttt tgatagcgat tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa
      601 agtggagaga gtccaagtcc gctctttcaa taagacggct aattttttct ctaatataat
      661 gattgcactc tttgctattt cctgtatttt gccatttata tttgttatcg ccatctcttt
      721 taccgatgag tccatattgt cgactcacgg gtacagtttt tggccagaaa agttcagtat
      781 atttggttat acgtacttgt tcgaccagat gcaggataaa attttccaag cgcttttcgt
      841 cactgtactt gttactgttt taggtacact gatcaatagc acggctactt ctttatatgc
      901 ttatgcgatt tcacggtcaa actttccatt ccgtcgtttt tttaccgttt tttgtttgat
      961 tacgatgttg ttctcaccag ggatggtggc caattatctt gttatgacaa atcttcttca
     1021 gttaaaagat acgatttggg cattgatctt gccaatggct gtcagtccct tcaatattat
     1081 tgtgatgcgc acattcttta aacgttcggt ctctgattcg attattgaat cggcacggat
     1141 cgatggagct agcgaactgc gaatatttgt tcagatcgtt ttaccgttag ctgttccagg
     1201 tattgcgaca atcagtttat ttgccgcatt gggttactgg aatgactggt tcaatgcttt
     1261 actctacatc caagatgata agctagttcc actacagtat cttttgatga aaatccaaag
     1321 taatattcaa tatctgacac aaaattcagg agcaggaagc caattgacag gcggattagc
     1381 ctcagttcca ggagaatctg ccagaatggc gatcgtagtc atctcgacgt tgcctattgc
     1441 gatcagctat ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa
     1501 agaataaaga aacaaaaata taaatcataa aattaggagg aagaaaaatg aagttatgga
     1561 agaaactagc ctttacaggt gtttcagcgt tgttgttggg aacattagca gcatgcggag
     1621 gttctggttc taaagaccag gcagaagcta gcaattcgga cactttgcag atgtatcaaa
     1681 tcggagataa accagataat ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa
     1741 aagagatcgg tgtaaaagtc aatatcaatt acattggctg gggcgattac gaaaagaaaa
     1801 tgaacgtcat catttcttcc ggggaaaatt atgatatcgc ctttgcgaac aactatgttt
     1861 ccaatgccca aaaaggagcc tttgctgatt tgactgaatt agcgcctaag tacgctgaaa
     1921 aagcttataa tgatttagat gaagcatata ttaaaggaaa tctggtcaat ggaaaattat
     1981 atgctttccc agtcaatgga aatgtctttg ctcaacaagt acttactttt aacaaagctt
     2041 tactagataa atacgatctt tcaattgatg gaatccaatc ttacgctgat gcagaaaaag
     2101 tcttgaagga attccatgaa aaagaaccaa acacggcagc ttttgccatt ggacaaggct
     2161 tcaaagtcca agggaacttt gattatcctt tagggaatac gttaccattt gctattgatc
     2221 tggatggaga tgattcgaaa atcatcaacc aatacgataa tgaaacgtat atcggactgt
     2281 tacgtacaat gcatagctgg tacaaacaag gactcattcc aagtgacgct gcaacgagca
     2341 ataaggatta tccactagaa ggaaatactt ggctaatgcg tggagaaaca caaggaccgt
     2401 atgattatgg cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa
     2461 ttactgttcc actaaaatct acagctcaag cacaaatggc aaattttgtc gtatcaaata
     2521 cttcaaaaaa taaagaaaaa tcagtagaac ttctagggct gcttaacagt gatccagaat
     2581 tattgaatgg gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc gatggttcag
     2641 atgataaaat caaattgcta gatggttatc agccaaatac acatatgtct gcttggaata
     2701 caggaaacaa caagattcta tatacacaag agtctattac ggatgacatg attgccaaac
     2761 gtgatcaatc gattgctgat gcagaaactt caccgatttt aggattcagc ttcaacactg
     2821 acagtgtcaa aacagaatta agtaatattt ctaatgtcat gaatcaatat ttagatggat
     2881 tgaatacagg gactgttgat ccagatgaaa cattaccgaa attgaaagac gctttggaca
     2941 aggctggcta tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag
     3001 aataataatg aataaagaca aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc
     3061 gaataaacgg tgttcaaaag ccagtttctc ggtattaagc taaatatatg taaaaatgtg
     3121 gaaagccata ttcacaaaat ttttcttact actcgaaact aagcggctat ttcacaaact
     3181 cttggtaaac ggtgttcaat cagctgaccc tcggtattcg ctcacaaaac gacaaaatat
     3241 gaggaactat ttttccgttt gtttctctaa taatcaggtc aaggtgctga ttgcccaacc
     3301 tttttttgta taatgagaaa gtataaactt aatgtttgga ggagaagata tgttagggaa
     3361 ggttttggaa gagttattta tccggatctg ggtcattata aaactaacct tgtatttctg
     3421 gatatataca tttgccggcg ggatcatctt tggattaggt gcggcatgga aaacagtcaa
     3481 tgaactattt tatttgtacg gattcgagta taaagaaatc acaataaaaa gaggctggaa
     3541 tatatataaa agaaattttt taagaggcaa tctgctattt agtttatttt tatctggtac
     3601 tgctttattg agttacaatt tatatttatc tgttcaaatc aaagggctca tttttttagc
     3661 tattgatttt attttacttt ttgccttgct ctgcctattt gctacttatc aatacagtat
     3721 gattttagat agtgaatata cgatctctgt tcctaatctg ctaaagctaa gttttatttc
     3781 ggttttttcc agcttttcgt cttttctaaa aataattatt ggtagcggta taattttagg
     3841 cgtgacttgg caattcaaag gattgatcct gtttggtgtc attggtcttt tgacggtttg
     3901 gaatggtacg atgacaacac attggcgtga agaattagat aaacagttgg aaagttatga
     3961 ataagtttcg gattctattt tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg
     4021 cggttatcat gatcggtgta acgatgactg ctaccgttat ttttagtatc catacttggg
     4081 aacaaaatca aaaacaggca gaaaacatga caagtgatgc cgttcaaagc acaagcagaa
     4141 tgctgaatga taaaacaacc ttgagtagga tcatcaaaaa ccagcttgta gggaattccg
     4201 aaaagatcga aaatgtgacg acctacttaa caaaacccat agatcagtat ctgatgtatg
     4261 tatacgaaca gcaaaacagt accgatgaat tagtttcttt cccaaatcaa atcaaagatc
     4321 tctacgcaaa ttatgaagaa ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt
     4381 attatgaatc ttctagagaa aacaaaggtg gggaaataaa aaaggggaca ccaaaattac
     4441 tagacgcctt ttatatcaag ttgcctatca cacaaggagg agctgagtca ggaagtattt
     4501 ttattggttt tcaaaagaaa gaactagata tgattttaga aaatctaacg tcattcaatg
     4561 gactctcttt atacatgatt tctggaacga caaatcgact atacacattc catgataata
     4621 aaatagctaa agaaacgttt gaaagacaag aaatgatcat cacagagagt ctgaagaaaa
     4681 attcacaatt gccgattgac gatctggaaa aaaacaattt tgttcaacac caagaactgt
     4741 ctgatgatta tcgtatatta gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa
     4801 aaaatcttgc cccgctgatc attggtatca ttattttaga caatatactg cttatctttc
     4861 tttataaaac attcaaacga tattcacagc aagtatctat cgtcatgcag gccatggatc
     4921 aaacagcagc agggaattta gagacgcgga tcgacataac aaacacggaa tatgaactga
     4981 aggaactttc aatcggtatc aacgaaatgt tggatagcat caatcagttt gtagaagaca
     5041 tctacaaatt agaaatcaaa cagcaagacg cacatatgcg ggcgcttcaa gcacagatca
     5101 atccacactt tttatataat acattagaat atatccgaat gtatgcaatc agcgaaggaa
     5161 gtgaagaatt agcagatgtt gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc
     5221 aagagaaaac gatcacttta aaagaagaac tggacttttg cgaaaaatat gtctatttgt
     5281 atcaaatgcg ttatcctaat cgagtagcct atcattttat gatcgatcct gatttggaaa
     5341 agatcgaagt acctaaattt gtgatccagc cattagttga aaattatttt aaacacggta
     5401 ttgattttac tcgattcgat aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg
     5461 taaggatcat catcaaagat aatggaaaag ggatgactga aaaacggcta aaacagattg
     5521 aagaaaaact gtctcatcct aaagtggaac ttcacggttc tatcggtctg caaaacgtga
     5581 atgaacgttt acgtgccagc ttcggcagca gttattatat gtcgcttgag aataatgaga
     5641 caggtggact gaccgtttct attacattca aggagggatg agcgtgtatc aagtgttatt
     5701 ggtagatgat gaatacatga ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt
     5761 aggctttcaa atagtagcaa cagcagagaa tgccaaagaa ggactagctg tacttgaaca
     5821 gcgacagatt gatttagtag taacggatgt gacaatgcca gaaataaatg gactggaatt
     5881 tattgaagcg gctcaaaaag aacgtcataa ttttgaattt atgattttat caggatacca
     5941 agaattcgat tatttgaaag gaggtatgca attaggcgcc gtgaattatt tgatgaaacc
     6001 cgtgaataag ttcgaattgg ttgaaagcct caagaaaatc aaaacacggt tagatcagca
     6061 aaatgaacaa aaaaatcaac aagaaatcta tcaagaactg ttattcagtc aatggttgaa
     6121 tgaagaattg gatgaaataa gtgaagaaga aataatagga caaataagtg agaagcagca
     6181 taggatagta ttgattcaac tggcaagaac tcatgatact cttatcaata gatggttgca
     6241 agaacggcaa caaatctttt actatcaacg aaattacgga gaccgtgtat tgatcacgtt
     6301 gttgttggag gcagatgaag ttgatggctt ctgttgtttt gttcaagaaa actttcctga
     6361 tcaggaatgg ctgatcagta tcggggaaga gacagattct ttggacaaaa tccctgaaac
     6421 attccaacaa gcgaaagaca atctacagtt gcatcaattt tatggagata aagacgagca
     6481 tgttttttac gcagtacaag cagaaacaaa agatcaaacc atcgattttt caagtttcag
     6541 acgcgtgctg caaaataaac aattggaagt cgctcaaaaa atgatcactg atttttttga
     6601 acagtttcag ttagcagcca tgatgccgga agatatacga tattccagtt ttttactgtt
     6661 tatggagatt caacgggaat tgattgatct ggaagatgag gaatatttac aaggaatcga
     6721 aaaaatccat caggcaaaaa cggtgcaaga acttcagcag ctacttctgt cattcatcaa
     6781 aaagcatcag agacagaaaa agtacagtga caatgtggaa aaagtaatcg taatcctgca
     6841 ccagcattat caagaaccac ttacactcaa agaagtgtca gaaaacctgc atttaaatgt
     6901 catgtatcta ggacagctgt tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa
     6961 ccatttacgt atggaaaaag cgaaacaact gcttttgcac agtaatcaaa acatcaatga
     7021 gatagcaagt gagattggct acaacaacac cacgtatttc tctaaattat tcaagaaaat
     7081 cgtcggcaga tcccctaaag agtaccgaga aaacagtgga gaatcataaa aaaaaagcca
     7141 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
     7201 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg gagttcaaaa gccagtttct
     7261 cggtattaag ccaaatttat gcaaaa
//
LOCUS       DAYYAR010000127         7058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000127 DAYYAR010000000
VERSION     DAYYAR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.129"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..775
                     /locus_tag="ACHAT0_002397"
     CDS             <1..775
                     /locus_tag="ACHAT0_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFM8452307.1"
                     /translation="YRDELFDWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRR
                     QTSSQEIDQLIHEMKTILKKADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTE
                     EKQATFKLLEGKDFKDQFVPIVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQ
                     RFDNEENRRRQEEQARKQIEAANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRT
                     LNVHVKTIKSAADIDELTAEINQKLKEQLQENTEITIQF"
     gene            839..4471
                     /gene="pglX"
                     /locus_tag="ACHAT0_002398"
     CDS             839..4471
                     /gene="pglX"
                     /locus_tag="ACHAT0_002398"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFM8452308.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            4587..>7058
                     /gene="pglZ"
                     /locus_tag="ACHAT0_002399"
     CDS             4587..>7058
                     /gene="pglZ"
                     /locus_tag="ACHAT0_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFM8452309.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERI"
BASE COUNT         2661 a          951 c         1333 g         2113 t
ORIGIN      
        1 atatcgcgat gaattattcg actgggctga aacatatgat aaaattaaaa atttccacaa
       61 gcaaaatagt actcagaaaa aaatctggga gaatgcacaa gataaaatta gattaatcga
      121 aaaaagtcgg cgtcaaactt ctagccaaga aattgatcaa ttgattcatg agatgaaaac
      181 cattttgaaa aaggctgatc cttattcaga tatgatgaaa ctaaatgagt tgattcaaga
      241 atttattgaa caatataatc aactgttaga gttggaaagt caggaaatat taaataaatt
      301 aacggaagaa aaacaagcaa catttaaatt attagaagga aaagatttta aggatcaatt
      361 tgtaccaata gttgaccaag aatttcaaga actgaaagca aaaattgaac aagcgaatac
      421 tatagatgat cttgttttct tatcattaaa cgtagcagaa cagtatttga tctttaatca
      481 acgtttcgat aatgaagaaa accgcagacg tcaagaagaa caagcacgta aacaaattga
      541 agcagcaaac ttgattcgtg aaactgaaaa agatgaaata gactctgcag caaaagctga
      601 agaaatagcg atagtaaaac ctactattca acagaaaaca gtaccaatga gaacacttaa
      661 cgttcatgtt aaaactatta agtcagcagc agatattgat gagttaacag ctgaaattaa
      721 tcaaaaactg aaggaacaac ttcaagaaaa tacagaaatc acgattcaat tctagtgaaa
      781 agacaggcat aaaaaggtgc ctgtcttttc aaaggttaca tatagaaggg aaagaaaaat
      841 ggataagaaa gcgataaaga atttcgcggt caatgcccgt agaaaattaa ttgatgaagt
      901 aatgactaaa gcaaaacgtt taggaattga tgaatcaaaa attgaagatg tgaagaaaat
      961 tgatagcaat ttacaagaaa tcgaaaaatc aggaataaga attcaaggaa aagaaatagc
     1021 gcaaagaaac aaattgattg cagaactaaa acaacgtgct tctgtcactt cttattcaga
     1081 agcttttcaa gagttaatcg aagaggttgc ttatacgtgg tttaaccgat tgattgccat
     1141 tcggtttatg gaggtaaatg attatttacc tgacacgtat cgtgtattat ctagtgaaat
     1201 aagtgggaaa attgagccag atattattac agatatttat gatgccgata tctttgaaga
     1261 attatctagt aatgaacagc aacaagtcag aacttggaaa acagataatt cagcagaagc
     1321 aatggacaag ctgtatcaat tgatttttat aaaaatctgt aatcaattga atgattactt
     1381 acctgagcta tttgaagaag tagaagatta tacagaactt ttgtttacag cttcttatat
     1441 taaaaaagat ggtgtaatcg ctgatctttt aagtattcca gaggatgatt tctatgttca
     1501 agctggtgga caggtagaga ttattgggtg gttgtatcag tactataata gtgagccaca
     1561 taatcaagtg gtgaatattt ataaagggat tgtcaaaaaa caagatattc cggcagcaac
     1621 tcaactattt accacagatt gggtcgttcg ttatatggtt gacaatagtt taggaaagta
     1681 ttggttagat cattatccca atagtgaatt aagaaataag ttgaaatact taattccaga
     1741 taaatatgct tttgaacaag caacatctgt aagtaattta aaaccagaag aactaaaaat
     1801 tatcgataac gcaatggggt ctgggcatat tttagtttac gccttcgatg tattgatgga
     1861 aatttatctt acgttaggat attctgagcg tgatgctgca cgtgcaatcg ttgaatacaa
     1921 tttatatggt cttgaaatcg ataaaagagc gtatcaattg tcttattttg caattatgat
     1981 gaaagggcgt caatacgatc gtcttttttt gaaaaaaagt ctatcgccga atttaagaga
     2041 gtttattgat tctcctaaag tgacagacga gtatcaagag cgtattagtg aattgaacaa
     2101 tgataagtat agtgaacgct ggaatgaata caaagagtta atttttgatc aatttgaaaa
     2161 tgggaaagaa cttggatcaa tcattaagtt agtcgataaa aataatcagg caataactaa
     2221 agaatactta ggaaaagtaa gagagtatat atcaaaattc aacgaattta cgaatatgga
     2281 tattctttat gggatgaagg aaatacaaga aaaatacttg cacattcttg acgtggctga
     2341 aatgctagtt gataaatatg tagcggtagt cactaatccg ccttatctta ataaaatgga
     2401 taagaaatta aaaaaatatg taaatgataa ctattctgat gtcaaaaaag atctattttc
     2461 tgtgtttatt aaattgaata gccaaatgct gattgataat ggatatgctt cctttatgac
     2521 gccatttgtt tggatgttca tttctagcta tgaaaagtta cgctcatttt taattaatga
     2581 aagaaacatt tctagtttga ttcaaatgga gtattcagct tttgaagaag caaccgttcc
     2641 aatttgtacg tttacgataa aaaattcaaa tgaacaagat ggaagctata taaaattatc
     2701 agattttaaa ggtggaatgg aagttcaaaa aattaaaacg ttagaagcaa ttcatcagca
     2761 tggtgaatgc gattattttt atagtactaa tcaagacaac ttcaaaaaaa tcccaggaat
     2821 gccgattgct tactgggcaa gcgataacgt aatcactgat tttgctcaag gaaaattaac
     2881 taacgatgtt gcagatgcaa aacaagggtt ggcaaccgga aataacaata aatttttgcg
     2941 gtattggttt gaagtagaat ttaacaaaat aaagtttgat gctaaaacat tagaggacgc
     3001 taaaaagtct agaaaaaaat ggtttcctac aactaaaggt ggtcgtttta gaaaatggta
     3061 tggaaacaat gaatacgtgg ttaactggga gaatgatgga gatgaaataa aaaattatgt
     3121 taatgaaaat gggaagttaa aatctagacc acaaaatctt aattactatt ttaaaaaggg
     3181 aattacttgg tcgacacttt catctggaca agtatctttt agattgcaga atcaggcaat
     3241 ttttgaaact aaaggatctg ttctgttctc aaaggatata actgactttg aagaaatatt
     3301 tggctatgta aactctaaat tagttaatca ctttttaact tttatttctc ccaccttgga
     3361 ttaccatgaa ggaccaatgg gaaaactacc ttttcttaaa gttacatctg agagaaaatt
     3421 aaaaaaaata agagaaaaag tggatgaaaa tatcgatata gctagaaaag actataatgc
     3481 tcttgaaacg tcatacgagt ttagaagaaa cattttgtta agttatatgt ttaaaaaaga
     3541 tctctcagag gcatttaact gtctaagtag gcagttggtt aatcaattta atactctaaa
     3601 atcgaatgaa gaggaactga acgacatatt cattaatcat tattctcttg aaaaagaatt
     3661 aacaaaagag gttcaagatg aatatatctc tgcaaggtta cctgataagg cgagggaaat
     3721 taaggatttt atttcttatt caataggttg tatcttcggt cgttactccc tagacgaaga
     3781 tggtctcatc ttcgctggcg gcgaatggga tccttcccgc tatcaaaaat ttaaacctga
     3841 tactgataat gttttactca tcaccgatga tgagtatttc aaggatagcc aaattgacat
     3901 tgtcaatcgt ttcattgatt tcgttgagat tacttttggt aaagaaaccc ttgaagaaaa
     3961 tctccagttt atcgctgaca ctttaggtgg caaagggaca agtcgagaaa ttattcgtcg
     4021 ttacttcgta caagattttt acaaagacca tttaaaaaca tactccaatc gaccaattta
     4081 ttggcaattg gactcaggaa aacaaaatgg ctttaaagcg ttaatgtact tgcatcgcta
     4141 tacaccagat caattaggaa aagtacgtac tgaataccta cacgaactac aaaaagcgta
     4201 tgacaatcga atcgaattgc gtcagcaaca aatttcaaca agtgataacc caaaagaaag
     4261 aaatgcaatg acaaaagaaa ttgtaaagat tcagaagcaa ttaaaagaag ttcgtgagta
     4321 tgacgagaag ttgggtcact tagcattaaa acgaattgaa ttagacttag atgatggtgt
     4381 aattgtcaat tatgataagt tgcagcgtga tcctaaaacc ggcgagaaat tccaaatttt
     4441 tgcaaaaggg gtcaaagaac caaaaaaata gttatctaag aaagttgttt tctaacaact
     4501 ttctttttta ttcttaaatt gcgtgcgaaa ttatccaaat tatgtcaata taaggggtat
     4561 agaaagagaa cgattaggtg agattgatga cagatataaa tacgcaacaa attgaagaaa
     4621 aattaaatca acgttttcct aattatggat gtaggaaaat cattttttgg tttgattcaa
     4681 atcaggattt tcttgaagaa attgactcat tggtcattca aaatgcaaaa atctatcgat
     4741 tagaaaagga cgcacaattt aaaactaagc gtttattgga atatgaagat cccgaaacta
     4801 actatttaat ctatgcgcca tttcctcgtt gggatgataa agatgaagat aatcatttgt
     4861 tgagtgtatt gaattattca gaggaattta gtgcagaccg cttagcgatt atcatgaatg
     4921 aattggctat tccacttaga tttcatcaga cagttaaaca gtatcgtaat ttctttaatg
     4981 caaaggatcg catgagaagt tttggtgaat ttagtacgca tatgacaatt aataaatcag
     5041 aaacgattga attaatgata atggctgttt tgttgaaatc gaaaagtatt cgattaaatg
     5101 atttactgcg tgtagttcta aaaagcttta cagaaagtag ggagaatcct ttaagggaac
     5161 ttgataagta tcagttaaca gctaaatttt gggaatttat tcaaatgaaa tttggcttta
     5221 cggatagcca gccaacattg gaaaagctga cgatgtgtct ttatttgaac tatttttatt
     5281 atcaaattga tcaagagatg ccgaagaagt tcaaaggatt tgaaatacta catttgaaag
     5341 caaacattat tactttcatg gatcagttta tgaatgatac acgttacatg gaaagctttg
     5401 aacagctatc taaaaaagta tatcaggcta ttgatggtga tggattatta gacagtatag
     5461 aaattgatga tctaattgaa gcagacgtat tttctgagat tcatgcaaaa atattaatgt
     5521 tttatgcaga acgattaaag gcttctgaca ctacaacgaa aattgcagga caaacaattt
     5581 ttgatagttt aaagcagcgc gaaaaaatgc atttcgcatc ggattatatt tatgagtatc
     5641 aattattgaa acatgcttat tatcttgtgg ataaaaacag attgtgcttg ccgatgaaca
     5701 tggataattt gctaactcaa tatgagaagg aaatgtattg ggttgatacg cattatcgga
     5761 aatttatttg gaatagtgat catatagaag aaaagcaaaa atatggccaa ttgtatcgat
     5821 tagttgaagc aaattattcg aagtttcttg atcaatcagg acaaatttgg aatcaaactt
     5881 ttgaatttaa gaatcgtcca tccattcgaa aattttataa tcaagctgta atgaaacgct
     5941 ctgttaaaac tgtagttatt atttcagatg cttttcgata tgagttaggg atgaatttag
     6001 aacgtaagtt agttagagaa aagaagtatt caacaaacat ggctagcttt ctttctgtat
     6061 taccttcagt tactgaattc ggtaaagccg ccttattacc aaacaatcaa ctaacttaca
     6121 atgaaggaac agaagtactg attgatggga aaaaaacgaa tggtttatcc aatcgaaatg
     6181 cgattctcca gcaacgagat gcaaatgctg tagctttaaa ttatgatgat gtggttaaga
     6241 tgaataaggc tgaggtacga gaagtattta atggcaaatc agtcatttat atttatcacg
     6301 atcaagtgga tagaacaggt gatcactcga atgaaaatcg agtttttgag gcctgtgatt
     6361 tagcgataag cgaaatttcc cgacttatta atcgcttgca taatgatggc agtatctatc
     6421 gttttatagt tacggctgat catggattta tttataaaag aagccacgtt gaagaagtag
     6481 ataagattga aaatccatct agaaatgaaa aagatcgtgt agaacgtcgt tttattctta
     6541 gtgaagaaga atacgaaata attggtacga aaaattactc tttgggagat tctttaggaa
     6601 atgatgacac tagaaacatt tgtgtcccga ttacttcaag tatttttaag aaagctggcg
     6661 ggggacaaag ctacgttcat ggagggtcat ctattcaaga aatgctagtc cctgttctag
     6721 aaattacggt agccaaaggt agcaatgcaa aggaaccagt agcaattgaa ttaatgacta
     6781 gcaaccgcag aattacagga ttatcaacca cgttagaatt ctatcaaaaa aatgcaatta
     6841 gtgatgttta tgaccccgca tctttctggc tttattttga agatgaatat ggtgaacgta
     6901 tctcaaatga agaaatctat gttgcggatt caaaaacaga agatgcaaat cagcggttta
     6961 ctaaatttgt ttttgaattt gtaaatcgtc gatatacagt tgaagatact tattatttag
     7021 tgataaaaaa tcaaaaaaat gatttgaccg agcgaata
//
LOCUS       DAYYAR010000128         6927 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000128 DAYYAR010000000
VERSION     DAYYAR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.130"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            389..1045
                     /locus_tag="ACHAT0_002400"
     CDS             389..1045
                     /locus_tag="ACHAT0_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452310.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            1224..1789
                     /locus_tag="ACHAT0_002401"
                     /pseudo
     CDS             1224..1789
                     /locus_tag="ACHAT0_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2488..3009)
                     /locus_tag="ACHAT0_002402"
     CDS             complement(2488..3009)
                     /locus_tag="ACHAT0_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8452311.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            3207..6887
                     /gene="nifJ"
                     /locus_tag="ACHAT0_002403"
     CDS             3207..6887
                     /gene="nifJ"
                     /locus_tag="ACHAT0_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8452312.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
BASE COUNT         2261 a         1132 c         1517 g         2017 t
ORIGIN      
        1 gattttagtt ttgtacataa atttttgctt aatatagaag tcctgatata gataagatca
       61 agtatttatc tataactggc ctgattaatt aaaaaactgg aaggaactgc tcgatcatcc
      121 ttgagaactg ctcttcttac ttctggtcaa gattctcgtg gttggtggat aaggatggtt
      181 gataagttca ttggattagc tagtcgaaaa caaccaggtg taattaatgt agaagtttta
      241 acaaggggat ggagtgattt attcagtgat aatcataagt tcataaatag taattgagat
      301 gtatggttgg aatacgttat acctgaactt gaactaccaa gatttggttt agaatttgag
      361 aatcgatgtt tataaaggga gggcaaaaat gcgttttttt acaactttta ctatcattat
      421 gattgcagta ctttttattt ttctagatat agcaaagcga aatacagcat ttttattata
      481 tcgggtttta cttcgagcag gtctcataac atttatatcg atcgttggat tttttctatt
      541 cacagttatt gtatttattt ggagaactcc agcaccacca ttgccagaga ttacatatgg
      601 agaatttcct tttagacttg agtatgaatt gaatgaagaa ttacatgtga tagaagacac
      661 gttaatagtt gaatttgatg gatttggaat gaacgaaggc atagggagat atcgtagatg
      721 gacaagtcgt ttagccagcg gagaagattt agtgttgctg ttagaagtta gtgataataa
      781 gcaaatattt tattttccag gacctgctaa ttattacatg ggagataggt tgaatggtta
      841 taatcatact tttcctagtg cttctttcat tgaaagggaa agaggcatta ctagaaggga
      901 tattttacat gacaaagaat tattagaaca atttggtccg cttgatcaaa atacaataaa
      961 tgaagaagaa ttgttaaatc aatataatat aaggctagtg aattgggaaa tatctgaacc
     1021 gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca agccaaatca
     1081 tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa ttcatagcga
     1141 atattattac gatcagtaga tattttgata ttaaaggagt attacgaact agtatgtgag
     1201 aatgtagagg tgatttgcga ataatgagaa atttatcaaa tagaaacaaa atattaatca
     1261 ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt agaataatat
     1321 tgggtcctaa gccaccaaga cctgcgatta cccgagggga gtttgatttt agattagaat
     1381 atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta tttgatggat
     1441 tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtttgcat ttggcgagtg
     1501 acaagagggc acaacaatta ttgttacatg agttagatga tggtcgaaaa atatattatc
     1561 attttggagg agctggattt tatatgggga agttagtagc agtgtaactg taacaaggaa
     1621 agatttgcca ggtcttggca ttcgaaactc catattttat atcgagcgtg ggagagatac
     1681 atctattggg aacaggaaag ggttaactct tgaagagtta tataatgact ttggaatccg
     1741 tcttgttagt tgggaacaca atccaccaat agaaaatcgc tttgaataat cgaaatctat
     1801 taatccattt catcagccag tgaatgttgt aatggaacaa tttggtttag tgttcaaaac
     1861 cattgaaata ctaggctgtg atcagcaaaa atgtatctga ataaaaaaga gagcaactat
     1921 taaatgagag gagttaggag ttgttagctt tatacgaatc aaagtggcaa atacagatgc
     1981 gtatgcattt gtgtttgcca tcttgcgttt ggtagacgag ataggtataa gctaaagaaa
     2041 caacttgttt aaaataaacg acctagctca acaaacgacc atctaatcaa ctaacagcat
     2101 acataaaagc aactattttt gaagtagaat tgaaatcggg ataacttaaa aagtgattgg
     2161 gatactagtg tgaatcaccg tacataaata ttactcgtta agggaatttg agaaattttg
     2221 tatgaaaatt taagaattag ttagtatgcg caagtcaaaa atatgaataa attatttggt
     2281 ggagataatt tgctcagctt gtcataagaa atgagataat ctggcagatg caagcttttg
     2341 gacaccattt attcggaata aggaggctgg gacagaagcg tttaactcca agaaataaga
     2401 aggagttgct tctgcttccg ccgtttatag gtgtttagag cgtgagacaa aagtgttctt
     2461 tacttttgtc ctacgctcct tattttttta ttttgtttgg atcaattctt ttaattcctc
     2521 tattgaaata gattcatcaa agatttcttt acccaagatg acagtcggaa caaatcgaat
     2581 atgtgcttgc tcagcttcgt tgatgacagc ttggctcagc tgttcgtctt tttgttctgt
     2641 cagctttagt ttatctactg caaaatccgc tacttcttgg agagaaagat gtttccactc
     2701 gtcttgagta tcgaatattt gtttgatctc tttgatcgct ttttgaccat cgctgattgt
     2761 gagataccgg tgcatcacgt ttcctcgctg caagctttct ttttctttat cgaacaattt
     2821 gatcactcgc tgcaattgcc cgctttggac tgcttcttct aatagctcat aagattcttc
     2881 aaaccattgt cggcaatacg gacaagatag gttgatgaat tcgaccattt ttttaggagc
     2941 attcgatgaa ccataaagga ttcctttttg tgtatttgtt ttcgttgcat caatcactga
     3001 tatatccatg ttaaaacacc tctttcgctt atagtttata catgtctgac aaaaaaaggc
     3061 aacgatttag actgtttgtc taaaaattag atgaccaatt caaatagaca tatgtgaaat
     3121 atttttataa ataaaagaaa aaatggaatg ttttacgtta taatggaaca gataataaga
     3181 gagaatatga acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg
     3241 cagccgcata tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca
     3301 gttcgaccat ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aatgtattcg
     3361 gtcaaccagt aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg
     3421 gatcattgaa aactggtgca ctgacaacta catataccgc ttctcaaggg ctgcttttga
     3481 tgataccgaa tatgtacaag attgctgggg aactactgcc aagtgttttc catgttgcta
     3541 gtcgggcagt gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg
     3601 caagacaaac aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag
     3661 cacctgttgc ccatcttgct tctattgaag gagaagtacc ttttttgaat ttctttgatg
     3721 gattccgtac gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc
     3781 ctctgatcaa tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc
     3841 cgactgttag tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca
     3901 acccctatta tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat
     3961 tacgaggaac ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca
     4021 ttgtttctat gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa
     4081 acggacggaa aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat
     4141 tcgtgcaaaa gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac
     4201 caggggcagg tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac
     4261 ttcgcccggc tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc
     4321 aaatctcagc agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta
     4381 ccatagggat cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt
     4441 tgacagaacc gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg
     4501 gagcaaataa atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct
     4561 atttttatta tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag
     4621 atacgccgat acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac
     4681 cagcttacct gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat
     4741 tgaatactct ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt
     4801 acttggctga aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag
     4861 tcggtttggg acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca
     4921 tcattccttt tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac
     4981 ataaatcaat ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc
     5041 ttcatcaagt cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata
     5101 aaaggaacac tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc
     5161 aactctctgt tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa
     5221 cggcttctat ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct
     5281 gtacgatgtg taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag
     5341 cagatgaaga ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg
     5401 cagacggcgt aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt
     5461 gtgtagaggc ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga
     5521 aagaacaagc tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca
     5581 aaggaaagaa tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg
     5641 cttgctcagg ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc
     5701 gaatgatgat cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt
     5761 ctccttatac gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag
     5821 ataatgctga atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg
     5881 caagttcaat caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag
     5941 actggttggt acacatgttt gagagcgaag gaacgcagca aagagcagca aaactgcaag
     6001 ctgcgatcga aagcgaacga tcaattgtcc ctgaactgga ggatatatac aagagaaagg
     6061 atctattcgt aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg
     6121 gttttggcgg gatcgatcat gtacttgcca gcggagcaga cgtgaatatt cttgttttgg
     6181 ataacgaagt ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa
     6241 tcgctaaatt ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga
     6301 tgacatatgg aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcagacga
     6361 taaaggcttt tgaagaagca gaaaagtatc ctggaccttc tttaatcatt gcctatacgc
     6421 cttgtatcac acatggtttg cttggtggaa tgaaagaatc gcttaaagaa gcaaaagaag
     6481 ctgtttcttc tggctattgg tcattatatc gatataaccc tgcaatggtc gaaaaaaaca
     6541 agaacccaat gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga
     6601 ggaaacaagt tcgcttttcc tcgctagaga acgcaaatcc aaatcttgca aaagctttat
     6661 ttgaaaagac tatccaagac gctaaaacac gtttttacaa ctatgctgca ctgacgggag
     6721 aattagaaaa aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga
     6781 ctagaaaaga aaaaacgacg gaccttgaag cagaagaaag aagagctgcc agagcagaac
     6841 gacgtaaaaa aatggaacaa gctacaaaag aaaaaaagga aaaataacag aaaaaatata
     6901 atgaataatt ctctgaagtt taaaaat
//
LOCUS       DAYYAR010000129         6870 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000129 DAYYAR010000000
VERSION     DAYYAR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.131"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(393..3098)
                     /locus_tag="ACHAT0_002404"
     CDS             complement(393..3098)
                     /locus_tag="ACHAT0_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8452313.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3404..4351)
                     /locus_tag="ACHAT0_002405"
     CDS             complement(3404..4351)
                     /locus_tag="ACHAT0_002405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8452314.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4541..4810
                     /gene="rpsN"
                     /locus_tag="ACHAT0_002406"
     CDS             4541..4810
                     /gene="rpsN"
                     /locus_tag="ACHAT0_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8452315.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4986..5348)
                     /locus_tag="ACHAT0_002407"
     CDS             complement(4986..5348)
                     /locus_tag="ACHAT0_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8452316.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5359..6480)
                     /gene="alr"
                     /locus_tag="ACHAT0_002408"
     CDS             complement(5359..6480)
                     /gene="alr"
                     /locus_tag="ACHAT0_002408"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8452317.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6496..6846)
                     /gene="acpS"
                     /locus_tag="ACHAT0_002409"
     CDS             complement(6496..6846)
                     /gene="acpS"
                     /locus_tag="ACHAT0_002409"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8452318.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT         1809 a         1476 c         1267 g         2318 t
ORIGIN      
        1 attctataat aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc
       61 ttaacttttg gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc
      121 atagcgcagt tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat
      181 aagcccaacc aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga
      241 tgcagagcag cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta
      301 ttagctcaaa aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc
      361 aggtcaaaac actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc
      421 gcttctttga atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt
      481 ccaaagacaa caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa
      541 attcctcctg cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg
      601 atcatttgga agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc
      661 gacagtacta agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag
      721 acatagacga tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc
      781 ggattttgga catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc
      841 gcaatcgcca ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa
      901 ttgtttcggt ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca
      961 gcgccgaccc acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg
     1021 attggataaa gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt
     1081 gttgcgtcta gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt
     1141 cggatcgcgt ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc
     1201 aagtccgcag cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc
     1261 aatgtaccgg tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc
     1321 aatcctaatt tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg
     1381 atgccattga cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct
     1441 tgtccaattg caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca
     1501 gttgctttcg ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg
     1561 ccttgtgcag tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg
     1621 atttgacctg tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt
     1681 tgatctagca attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca
     1741 gataggcggt tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt
     1801 ggctgtaatt tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta
     1861 tcgattgcat tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg
     1921 tttttctttt catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt
     1981 aacgaacctg tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt
     2041 gtgatgcttg gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga
     2101 tcacttgcct gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg
     2161 atcgtcaagc cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca
     2221 aaatcgccgt cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct
     2281 tgcaaatcaa ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca
     2341 acgacttttt cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta
     2401 ttcagttttt ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag
     2461 ccgtcgatcg tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata
     2521 cttactaggt tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa
     2581 ctggctgttt tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg
     2641 gtgattttcg gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc
     2701 ttgatatcac cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata
     2761 cctgcgtaat actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat
     2821 tgccatccta attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact
     2881 tctttacctt gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca
     2941 gtatttccat atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg
     3001 atcatgatcg cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca
     3061 agcaaatata atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt
     3121 tcagctgaat tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag
     3181 tcgaattttt ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat
     3241 aattaaggat tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca
     3301 aagtttttat ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct
     3361 acaaaaatgt ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca
     3421 ggatgatttt cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct
     3481 taagtgaagc agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt
     3541 tgattatgcc agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat
     3601 aaaagccttc ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc
     3661 tgccgcctgg tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat
     3721 cgattacaag gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag
     3781 caaatgctga agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta
     3841 agattcggat tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg
     3901 ctcctagtat cattacagta tctgtcggct ggatttccgt caaatggttg attagattat
     3961 aagccgtaat tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa
     4021 tttttgctac ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat
     4081 ggacagctac tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa
     4141 taccagcgcc atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt
     4201 ctcctaaacg caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa
     4261 catgtccaga accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt
     4321 ctccgtaacc gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt
     4381 acgtttatgc ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa
     4441 caaaatagtt gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt
     4501 acgatttacg ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat
     4561 tgccaaagca aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt
     4621 aaaagcgcaa aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg
     4681 attgaaaaat cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat
     4741 gtcccgtata aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc
     4801 cagctggtaa tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg
     4861 gatcatcaaa aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg
     4921 tatctttttt tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc
     4981 tatatttatt tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg
     5041 tcaacttctg ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta
     5101 tcgatcgttc tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta
     5161 gctttgatcc caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca
     5221 acaatgatcg tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt
     5281 gttccgcctt gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt
     5341 tttaccatag gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca
     5401 agccacttca tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt
     5461 gtctgcattt ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg
     5521 gatcatcagt tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa
     5581 tagagaaaaa ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc
     5641 gatccactct gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg
     5701 gagttttttg acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac
     5761 ttccggtagt gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat
     5821 attgccgatt gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg
     5881 aggaagttcg ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata
     5941 tgtatcatct gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac
     6001 acgactttct ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat
     6061 acgacccatc ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt
     6121 ctctaaaaca gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc
     6181 atgagcaaca gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg
     6241 agtaaatcca gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga
     6301 agctccacct gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc
     6361 ttttactact gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt
     6421 ttcagttata gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat
     6481 cgtttttcct tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg
     6541 agtaatactg acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt
     6601 ttcatttttt aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc
     6661 tttagagaaa gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa
     6721 aggcaaggac tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg
     6781 tttttcttcg atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt
     6841 tatcatatgc ttttctcttt tcaataaatt
//
LOCUS       DAYYAR010000130         6812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000130 DAYYAR010000000
VERSION     DAYYAR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.132"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHAT0_002410"
     CDS             complement(<1..182)
                     /locus_tag="ACHAT0_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8452319.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHAT0_002411"
     CDS             517..951
                     /locus_tag="ACHAT0_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8452320.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHAT0_002412"
     CDS             974..1294
                     /locus_tag="ACHAT0_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452321.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHAT0_002413"
     CDS             1435..1626
                     /locus_tag="ACHAT0_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452322.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHAT0_002414"
     CDS             complement(1697..1954)
                     /locus_tag="ACHAT0_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8452323.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHAT0_002415"
     CDS             2112..2375
                     /locus_tag="ACHAT0_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452324.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHAT0_002416"
     CDS             2399..2587
                     /locus_tag="ACHAT0_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452325.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHAT0_002417"
     CDS             complement(2670..2855)
                     /locus_tag="ACHAT0_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452326.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHAT0_002418"
     CDS             3070..3441
                     /locus_tag="ACHAT0_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8452327.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHAT0_002419"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHAT0_002419"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHAT0_002420"
     CDS             complement(3709..4137)
                     /locus_tag="ACHAT0_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452328.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHAT0_002421"
     CDS             complement(4197..4955)
                     /locus_tag="ACHAT0_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8452329.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACHAT0_002422"
     CDS             5250..5447
                     /locus_tag="ACHAT0_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452330.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACHAT0_002423"
     CDS             5553..6035
                     /locus_tag="ACHAT0_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452331.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACHAT0_002424"
     CDS             complement(6093..6689)
                     /locus_tag="ACHAT0_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8452332.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2310 a         1053 c         1341 g         2108 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa ac
//
LOCUS       DAYYAR010000131         6644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000131 DAYYAR010000000
VERSION     DAYYAR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.133"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHAT0_002425"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHAT0_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHAT0_002426"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHAT0_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8452333.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHAT0_002427"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHAT0_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHAT0_002428"
     CDS             complement(2143..2373)
                     /locus_tag="ACHAT0_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8452334.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHAT0_002429"
     CDS             complement(2603..3421)
                     /locus_tag="ACHAT0_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8452335.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHAT0_002430"
     CDS             3582..4271
                     /locus_tag="ACHAT0_002430"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8452336.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHAT0_002431"
     CDS             4285..5787
                     /locus_tag="ACHAT0_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8452337.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHAT0_002432"
     CDS             5800..6276
                     /locus_tag="ACHAT0_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8452338.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHAT0_002433"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHAT0_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAYYAR010000132         6380 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000132 DAYYAR010000000
VERSION     DAYYAR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.134"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..402
                     /locus_tag="ACHAT0_002434"
     CDS             25..402
                     /locus_tag="ACHAT0_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8452339.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            390..728
                     /locus_tag="ACHAT0_002435"
     CDS             390..728
                     /locus_tag="ACHAT0_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8452340.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            862..1305
                     /locus_tag="ACHAT0_002436"
     CDS             862..1305
                     /locus_tag="ACHAT0_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM8452341.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1567..2382
                     /locus_tag="ACHAT0_002437"
     CDS             1567..2382
                     /locus_tag="ACHAT0_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8452342.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2392..3054
                     /locus_tag="ACHAT0_002438"
     CDS             2392..3054
                     /locus_tag="ACHAT0_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8452343.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            3259..4923
                     /gene="alsS"
                     /locus_tag="ACHAT0_002439"
     CDS             3259..4923
                     /gene="alsS"
                     /locus_tag="ACHAT0_002439"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8452344.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            4936..5646
                     /gene="budA"
                     /locus_tag="ACHAT0_002440"
     CDS             4936..5646
                     /gene="budA"
                     /locus_tag="ACHAT0_002440"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8452345.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            5776..6273
                     /locus_tag="ACHAT0_002441"
     CDS             5776..6273
                     /locus_tag="ACHAT0_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8452346.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         2085 a         1004 c         1357 g         1934 t
ORIGIN      
        1 gaaattaaga gaaagagtga tcagatgaat atcaaacaag tcagtgaaga aaagggcatt
       61 tcggccgata cattgcgcta ttatgaacga atcgggttga ttccaccagt taatcggaca
      121 aaaggcggaa tccgtgatta tacagaagaa gacctaaaat gggtcgactt tacgatctgt
      181 atgcgaaatg ccggattatc gatcgagtct ttgattgaat atattcgcct atctagcgaa
      241 ggagaagcaa ctgtatttga gcggcgtaag cttttgattg aagaaagtga acttttgacg
      301 aaaaaaattg ctgaaatgca agaatgtttg gaacgtttac aaggaaaaat caagaaatat
      361 gatcgtgtgt tatctagaaa taaaatggaa tgtatcaagt aaaaaaatca aggagcatcg
      421 ctatggagtt acgcttacta aaatattttt ggacaattgc agaagaagga acaatctctc
      481 aagccgcaga agttctacat cttacacaac caaccttaag ccgacaactg aaggagttag
      541 aagaggaact aggaacagaa ctgtttgtac gcgaaaaccg caaaatgatc ttgacagaag
      601 caggttattt tctaaaaaag ccgagcagaa gaaattttag atttaacgaa tcaaaccact
      661 caagaattcg agaacagaaa aaaacaatta ttcagcggcc gatttacgat cggctgtgtg
      721 gaagctgata attcgaatac attggcacta ttactagaaa agttgatccg tgattatccc
      781 agcgttcaat ttcatatcca cagcggaaca agtgatgata tcacggagtg actagaaaaa
      841 agcctgttag atttagcgat tttgctagat ccagtagata cagagaaata cgaaacgatt
      901 ttgttaccaa gaaaggaaaa atggggattg ctcattttag aaaattctgt tttagcaaaa
      961 aaagacgtga taacaaaaga agactttgcg aatgtacctc ttttgatctc aaagcgtcca
     1021 gaaatacagc gaatgtttgc tgactggtta ggcataaaaa aggagcagtt gctgatcaca
     1081 ggaacgtaca atttgatttt caatgtattt gcgctaattg agaatcaggt aggtgctgct
     1141 ttagtcatag aaggtgctgt aagtcatctt tcaaagcgag tgacattcgt tccgcttgac
     1201 ccaatgattg aaacgaactg catgctagtc tggaaaaaga atcggatttt atcacctgta
     1261 gtcaatgaat tgattcaccg tttcaaagaa gcttttgaac agtaataagt atctctaatc
     1321 gaagaaattt caatacaaaa gcgagacgta tgacagtctt gcttttgtat tttttcattt
     1381 ttaaatcaaa aaaaataaag agatttacat aacttgttaa ataagcgctt acaaacataa
     1441 gaagaacgga aatgaatcag ctataataac cgttgttctg gattgtaatc attacaaaga
     1501 aatgaagatt ataactttgg aggtgttcga aatgaaagaa aaaaagcaaa aattcgttca
     1561 aaaaacatgc cctttattca atggttgggt atgattctgc tgattgtatt agctttattg
     1621 acgatcattc cttttttatg gatggtttct gcttcattca aagcaaataa tgaagtgttc
     1681 acgattccga ttcaatggat ccctaaaact tggcatttag agaattatct ggttatttgg
     1741 tctcgtattc ctttagtgac attttttaag aatacagctt ttttaagcgt tgtgatcacg
     1801 ttgattcagt tgtttacttc tagttttgct gcttacgggt tttctaaact aaattttaaa
     1861 gggcgcgata ttttattttt agcatatatc ggcacgattg ctgtcccttg gcagtcttat
     1921 atgatcccgc agttcattat gatgagaaaa gtgggtttaa catatacgtt gtggtcgttg
     1981 gtgctattac aagcgttcaa tgcttttggt gtgttcttgt tgaagcaata ctacagtagt
     2041 atcccagatt ctttatgtga gagtgcgcgg atcgatggct tatcagagtg gggaatctat
     2101 cgcaagatca ttttacctct tacaaaaccg gcgattgcca gtttgacgat catcacattc
     2161 gttaatacat ggaacgatta tatggggcca ttcatttatt tgtcttctac ggaaaataaa
     2221 acaatccagt tagggttgaa aatgtttatt gggctatttg atgcagagta cgcattaatc
     2281 atggctgcct cagttgtttc tgttcttcct gttgctttta tatttttagc gatgcaaaaa
     2341 tattttgtag aagggatcgc ttcatcagga atgaaggaat aggaggtgat tatgaaagag
     2401 cagcgttttg acaaaaatgt ttatatggaa ttcttttatt ggttgtatca atcattggca
     2461 agtagccttt gtttttggtg tgtcaatatc ttgtatatag cagctgcgtt ccttttgtcg
     2521 atagatggaa ggaatgtctt ttcctttatt ttttctttac tttttattgg tccaggttgc
     2581 atcgtgatga taggtatgac agaagaatta gcaaaagaaa aaagtttttc accagtacgt
     2641 ttgttcataa agacgtttcg ccaattttgg ctaaagggat ttttttattg gctatttgct
     2701 tggatagtta gtgtgataat gatttttgat tgtttctttt ttatacgttt ttcttatgga
     2761 aaatggttga ttccgttgtt tgttttactt gcttgtttat cagtttcctt tagcatcaac
     2821 tactggtatt ttcaggtgag aaatccagcg tcgaaaccaa atcaagttct acggatagcg
     2881 ttctactata cgttgaaaaa atggtatgtg agtttactag attttttact attgacaagt
     2941 ttgtttctct tctttttcgt aaagccccaa tggtgtattc tactaggtcc ttctatcgta
     3001 tttggtttga tttacttcaa caatcgtaaa ttgatgagaa ctatggatct gtagacttta
     3061 taaattgaaa ctttcaatta agaatgacag tttttatgta aaaactgtca ttctttttca
     3121 ttttttgaaa catatggcta aacaaatgaa ataaaacgtg ctatactttt gaatgaaaca
     3181 ggtttagaaa ctgtatcaat tatttgtgat tatttctaac taataattaa aaataaagca
     3241 taaaaaggac gatgttcaat ggacgaaaaa atgaaacaag gttcgaaatc tgtagtagaa
     3301 agtttgatta atcatcatgt tgattatgta ttcggtatac caggtgcaaa aatcgatggt
     3361 gtatttaatg aattagagga tcaaggtcct gaattaatcg tgactcgtca cgaacaaaat
     3421 gctgcattta tggcacaagc aatcggacgt attacaggag agccaggagt agttatcgca
     3481 acaagtggtc ctggtgctag taacttagct acaggtttgg taacagcgac agcagaaggt
     3541 gacccagttt tagcaattgc cggacaagta aaacgaagcg atctattgaa gttgacccat
     3601 caaagcatgg ataatgcagc actgttccag ccaatcacaa aatacagtgc agaaatccaa
     3661 gatccagaaa caatttcaga agtgattgcc aatgcttatc gtatggctaa gagttcaaaa
     3721 aaaggcgcga gctttatcag tatcccgcaa gatgtagtgg atgcacctgt ccaagggaat
     3781 gtcatcaagc cattgagcga tcctaaatta ggttctgctt ctgcagatga tatccgttac
     3841 ctagctgaac gcattagaga agcaaaacta ccggtactat tagtaggtat gcgaggatca
     3901 agtgaaaaag aaacgttagc catcaggcaa ttagttggaa agacagcatt gcctgttgta
     3961 gagacattcc aagcagctgg agtgatctca cgtgaattag aagcgcattt ctttggtcgt
     4021 gtgggtttat ttagaaacca accaggagat atgcttttga aacgaagtga tctagttatt
     4081 gctattggct atgatccaat cgaatatgaa gcaagaaact ggaatgcgga aaaagatgcg
     4141 cggatcattg tgatcgatga agcgccagct gaaatcgatc cttttatgca gcctgaaaga
     4201 gaattgatcg gtgatatctc agccactctt gatttattga caggttcatt agagcctcaa
     4261 caagtgtcag aagatgcaaa agaatattta gcatctcttc aagcagaatt aacggagcgg
     4321 gacattgttc aatcaaaagg agaagcaggt atcttgcatc ctttagaagt tattaacacg
     4381 cttcagtcaa aagtaacaga tgatatgact gttaccgtag atgtaggtag tcattatatt
     4441 tggatggcac gccactttag aagttatgaa cctcgtcatt tgttattcag taatggtatg
     4501 caaacgcttg gtgttgcatt gccatgggcg atttctgctg cattggtgcg tccaaataca
     4561 caaatcgtat cagtttccgg cgatggggga tttttattct ctgcccagga tttagaaacg
     4621 gcggtacgta aaaaattgaa tatcgttcat ttgatttgga atgatggaca ctacaatatg
     4681 gtagagttcc aagaaaaaat gaaatatcag cgggcgtcag gtgtagactt tggtccagtt
     4741 gattttgtga aatatgcaga agcttttggc gctaaaggaa tccgagctac aagtgtagag
     4801 gaattagaaa aagctttaga agaaggattt gcgacagaag gacccgtaat tattgatatt
     4861 ccgattgatt accgtgataa tgagaaattg ggagaaacaa tcttaccaga tcaattctat
     4921 taaggaagtg tcgacatgac tgaaaaaatc ttataccagc acgggacatt aggagctttg
     4981 atggctggct tgatggatgg aacagaaacg attgctcata tattagaaaa aggaactctg
     5041 ggattgggaa cattgcatgg gcttgatggt gaagtcatct ttttagatgg cgtagcttat
     5101 caaggaagat cagatggctc agttgcccaa ttagatggaa gtgaacttac cccctatgca
     5161 gcaattacag attttacgcc agatactagt ttttctgtct caaaaactgc agatggagaa
     5221 caattgaaaa aagacatatt agtaagagaa gccggggaaa acctattttt ggcagtgaag
     5281 ataactggat tattcaaaaa tatgcacatc cgtatcatgc cgaagcaaga aaaaccttac
     5341 agaagactag caaaaatttc agagtctcaa ccagaattcc aacaaagcaa tgtccaagga
     5401 acattagtcg ggttctttac gcctgaatta ttccaaggag ttgctgcagc gggatttcac
     5461 ctgcatttta tcgatgatac acatacattt ggcggacatg taatggattt tgaagtggct
     5521 gaaggaaaag ttgagattag ccgcatctcg tcccttgtac agcattttcc agttgatgat
     5581 ccgacttttg ttcacgaaac gattgattat gctgatttag cagcagaaat ccaacaagca
     5641 gaatagaaaa agacagcgag atgcgttcaa ggtgattaac ctgacgtatc tcgttttttt
     5701 gaggagaaaa cctagcgttt acaaaaaaac tgagctataa tcttctccat agatcaaaaa
     5761 ggagcggttt ttatcatgtg gacagaaaaa cagaaaaaag atgcatacga aattttatta
     5821 ttacagcaac aaggattatt agaagcagaa gataactggt tagcaaattt agcaaattca
     5881 tctgcattgt tgaatgagac tttgcctgag actgtctttg caggatatta tttattcgat
     5941 ggtaatgaat tgattttagg accgttccaa gggcgagttt cttgcacacg tatcaagatg
     6001 ggaaaaggag tttgtggaga atcagctgaa aaacaagaaa cattgatcgt tgatgatgtc
     6061 aaaacccatg caaattacat ttcctgcgat tcagccgcac aatcagaaat cgtagtgcca
     6121 atggtaaaag aaggtaattt aatcggtgtt ttagatatcg attgcggagt aacgaaaggg
     6181 tatgatgaaa tcgatcaagc atttctagaa aaatatgtag atcttttatt agcaaatttt
     6241 aatataaatg ctttttcatt tattaataac taaaatcgtc tattgaaaca aaaaagaaga
     6301 gaaatcaaga ataaaaagag ccggaaagac agtgggcatt tctgtttttc ggttttttta
     6361 tttttttata aaatcacaag
//
LOCUS       DAYYAR010000133         6268 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000133 DAYYAR010000000
VERSION     DAYYAR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.135"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHAT0_002442"
     CDS             complement(99..1229)
                     /locus_tag="ACHAT0_002442"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM8452347.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHAT0_002443"
     CDS             complement(1878..3080)
                     /locus_tag="ACHAT0_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8452348.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHAT0_002444"
     CDS             complement(3140..3343)
                     /locus_tag="ACHAT0_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8452349.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHAT0_002445"
     CDS             complement(3485..4552)
                     /locus_tag="ACHAT0_002445"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8452350.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHAT0_002446"
     CDS             complement(4610..4717)
                     /locus_tag="ACHAT0_002446"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452351.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHAT0_002447"
     CDS             4880..5692
                     /locus_tag="ACHAT0_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452352.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHAT0_002448"
     CDS             complement(6066..6257)
                     /locus_tag="ACHAT0_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452353.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1860 a         1286 c          996 g         2126 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacata
//
LOCUS       DAYYAR010000134         6230 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000134 DAYYAR010000000
VERSION     DAYYAR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.136"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..633
                     /locus_tag="ACHAT0_002449"
     CDS             133..633
                     /locus_tag="ACHAT0_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8452354.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="ACHAT0_002450"
     CDS             730..2577
                     /locus_tag="ACHAT0_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8452355.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="ACHAT0_002451"
     CDS             2580..3476
                     /locus_tag="ACHAT0_002451"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8452356.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="ACHAT0_002452"
     CDS             3526..3915
                     /locus_tag="ACHAT0_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8452357.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..>6230
                     /locus_tag="ACHAT0_002453"
     CDS             3902..>6230
                     /locus_tag="ACHAT0_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8452358.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2106 a          925 c         1336 g         1863 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DAYYAR010000135         6202 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000135 DAYYAR010000000
VERSION     DAYYAR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.137"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACHAT0_002454"
     CDS             complement(<1..210)
                     /locus_tag="ACHAT0_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HFM8452359.1"
                     /translation="MNILILSCGRRCELVNAFKTALNNNEMEGKIIGADCSELAPALY
                     MCDEKIVLPKVSESNYIEELIKVIKF"
     gene            complement(222..2039)
                     /locus_tag="ACHAT0_002455"
     CDS             complement(222..2039)
                     /locus_tag="ACHAT0_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8452360.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(2053..2817)
                     /locus_tag="ACHAT0_002456"
     CDS             complement(2053..2817)
                     /locus_tag="ACHAT0_002456"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8452361.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            complement(2845..3543)
                     /locus_tag="ACHAT0_002457"
     CDS             complement(2845..3543)
                     /locus_tag="ACHAT0_002457"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8452362.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3555..4334)
                     /locus_tag="ACHAT0_002458"
     CDS             complement(3555..4334)
                     /locus_tag="ACHAT0_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8452363.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4350..5294)
                     /locus_tag="ACHAT0_002459"
     CDS             complement(4350..5294)
                     /locus_tag="ACHAT0_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8452364.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5340..6119)
                     /locus_tag="ACHAT0_002460"
     CDS             complement(5340..6119)
                     /locus_tag="ACHAT0_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452365.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1613 a         1297 c         1122 g         2170 t
ORIGIN      
        1 aaactttatt acttttatta attcctcaat ataattactt tctgaaactt taggtaatac
       61 tattttttcg tcacacatat atagtgcggg tgctagttca gagcaatctg ctccaatgat
      121 ttttccttcc atttcattat tgtttaatgc tgttttaaat gcattcacta gttcacaacg
      181 acgtccacaa cttaatatta aaatattcaa tttttaatct cctatctatt cgaagcattt
      241 gcaaaagtga caatgtcttt agccaattgt tcatcgtctt gcggcaggct cttgacaaaa
      301 tccatcactt tttgtattga gtaacctttg atgtttccta caaagatttt ctcataaact
      361 gcttcatcat ttctttcctt atccaacaat agttcttcat atagtttttc ccctggacgg
      421 atacctgttt caatgatttc gatttcgtct tcgctatacc cgctcaatcg gatcatgttt
      481 ttcgctaggt ctacaatctt cactggttca ctcatatcta gaataaaaat ttcgccacct
      541 tttgctaatg cgccagattg aataactaaa cgactagctt caggaatcgt cataaagtat
      601 cgagtcatac gaaagtctgt tacagtgatt ggaccacctt gagcaatttg ttctctgaat
      661 actggaatca cactaccgcg agaaccaagg acattcccaa atctgacagc tgagaatttc
      721 gtacagcctt cttcattcaa tccagtcaca atcatttcag cgattcgttt tgttgaaccc
      781 atgacatttg gaggattgtt agctttatct gtcgatacca tgacgaagtt tttcactttt
      841 gctgctttag ctgcttctgc tacatttttt gtaccgaaaa tattgttttt gactgcttct
      901 tttggattat attccatcag tggtacatgt ttatgagcag ctgcatggta gacgatatct
      961 ggatgatatt gttccatgat ctcattgatt ttctcgcgat cttggatatc tgcgatgatc
     1021 ggtacgattt ctgtaggaca attttgatga tgcgtgcgta actcacggtc aatcaagtag
     1081 atcgagttct ccccatgtcc taaaagcaat agctttgcag gattgaattg aatgatttgc
     1141 cgacagattt ctgaaccgat cgatcctcct gcgccggtga caagaatgac tttgtctgtg
     1201 atctgatctt tgatggattc aatatccagc tttacctcat ctcttcccag taaatccact
     1261 acgtcaattg tttttaattt tgatacgcta attttccctg aagcaagttc ttctatagaa
     1321 ggcatcgtgt tgactttcac acgagcggat tcgactaact cgaagattcg tcgtaatttt
     1381 tttcttgata aggaagggat agcgatcgtt accatttgaa tgtcatattt ttcaatcaat
     1441 tccggaatat cttttaatgc acctaatact ttttttcctg aaagatacgt atttctttta
     1501 tttggatcat catctacaaa cccgacaacg tggatatctt gagccgtttt ggatccaaga
     1561 aagctgttgt ataaaatacg cccgccttcg ccagcaccaa caatcaatgt atttttagca
     1621 gaatctgctg atacgtatct catatttttt gtttcgatgt agatacgcca gatcaatcgg
     1681 ctaccgatga tcaataagag cgaaagcaga taagcaaaga taactaaccg taaactgtat
     1741 cgccgattaa tgaacagcag tatgaggata cttgaggcag cacttgcaga tagtgaacta
     1801 aaaattgcaa tgatctctct taaatttgta tagcgattga tccgagtgaa aactttgaat
     1861 aaactaccat aaaataaata aaaaccaatc gacaaaccga tagagattaa aataagatcc
     1921 attggtatag atacaaacgg cttcatgaat ttagctgctg caatatttgc agcaattaat
     1981 aacaagctgt ccacaaaaac caatgtggca atttttcttc ttcttgttaa tataaacact
     2041 ttttaattcc ccctaaaata aaccaaactt cttctttttt acctcttcat agtctggata
     2101 actgatttca tccccgttga tcagatcctt tgcaacttgc ttcatctgta ctactttttc
     2161 cttaccaaag tccttggcaa tctgctcata gcactctttt aagtaaaagg tgcgcttaga
     2221 gactccgtgt gcatcagaag cagtcatctg cacgagatta tgtttgacca ttgttttagc
     2281 tgttttctga atatctttgc caaatacccc aacataactt ggcgcagtaa gttgggccaa
     2341 acaccccata tctaggaacg gaagcaaatg attcgggtct tcgcgaaaat gcgcgttgcg
     2401 ttctggatgg acgatcactg gtgtctttcc tagttctaac aactcaaaaa aaagccgttc
     2461 tgcatataac ggcacttcca gtgtcggaaa ttcgatcaga agatatgtat catctaaatc
     2521 tgtaaataaa atttcatctc ggtggatgtc ttcgatgagc gttccggtaa tacgtacttc
     2581 ttgtccttcc aataacgtca aaggcaactg tcttttctct aattcggctt gtaacgaagc
     2641 gactagagaa atgacttgtg atttttcatt gctgtatttt ccattattgt ggtgtggtgt
     2701 gcataaaata tgcgtgatgc cttgctgaat cgctttttca gccattgcaa tacttgcttc
     2761 taagttttct gccccgtcat cgattcctgg taggatatgg caatgtagat cgatcatttc
     2821 tcttgtctcc tctcttctta ttccttaatt tccgtagtaa taaccagcat ctttgctatg
     2881 ttctgctccg ttgtatacaa cacctaaaac tcgtgcttgt accatctcta gcagttcttt
     2941 tgctttattc aatgattctt tacgtgatgt attttcacgt acgaccaata aagtgccatc
     3001 cgtttttgaa gacatgatct gtgcatctgt tacggctact actggtggca tatcgaatat
     3061 caccacgtca aataactgtc ttgcttcgtc taggatctga tccattcttg gcgaacttaa
     3121 caattctgat gggttgggtg gcttaggtcc actaggcaaa atcgacaagt tctcgactgg
     3181 cgttcgttgg attacatctg ctacacttcc tgatgaacta agtgctgtac ttaatcccct
     3241 ggcattgttc agctggaatg tcttatagac gacgggttta cgtaaatctg catcgactag
     3301 tagaactcgt tgaccggatt tagcaaaaac gactgcaatg tttgctgcag tggtggattt
     3361 tccttctcca ggacctgatg aagtcacgac aatcgttttg atttgctgac cagctgcgga
     3421 ggcaaactgg atattcgtcc ggatcgtacg atattgttcg gcgattggtg aagatgggtt
     3481 tgttaatgtg atcaaactga ctgcatgagt ttgtgctgct ttttgtttct gtgttcgtgc
     3541 catttttctt cctcctagac tcttgagcgg cttctgcgtg atggggctgt tttatcgttt
     3601 gtagcttttg gtcgtgcagg agctgttcct tttttacgga taccagcgtt caattctttc
     3661 gctgacattt ctggaactgt tcctaagatt gggaatccta atgcatccgt cacatatttt
     3721 tcatctttca ctgtccgatc aagtaattcg aataagaatg ccagacctac gcctaccatc
     3781 attcctaata ctaagccaat cactagattc aatttattat ttggcgaaac aggtgtcgta
     3841 ttggcaactg cacctgaaat aatcgagatt ttatcgacat tcatcacgtc ttttgcgttc
     3901 tcttggaaaa cttgtgaagt tgtgttagcg atctgttgag cagtcactgc attcgtgctt
     3961 attgcctgga tcgagaacat ttgtgaattt tgtgactgat tgacggagat cgcatctttg
     4021 atttgtccag cagtcatttt cacattgtct tctgtttcta aacgatcttt gacttcattt
     4081 agtactaaat cactgacgat catatcttta tacgtattga tcatttgcag gttcgtattg
     4141 acatcattga catttgctgt gtctgattgt ggcagtgtca cgatcaattg tgcttgtgaa
     4201 ctgtattttg gtgtcaagat gaaaaatgtt acgattcctg agatggccaa gccagcaaac
     4261 attgagatga tgattgttac caagtgtttg cgtaatgttt caaaaatttc tttcaagctg
     4321 attgtctctt ccattttttc tccactttcc tatttattct ctagattcat ttgatctttt
     4381 aattcttttt tcacacgatc aagttcttga tcgctgactt tttgatagga gatattatct
     4441 tgcatgaagc cttctccttg cagctgatct tgcttgatcg taccaaatgc ctcccgataa
     4501 tctaaagcga tttttttcat ttcatcaaag ctcatgtctg ttttcatatt cgactctaga
     4561 gcattcaaaa cggattgata gttgctgaca ccgtttaaac tcaatacttt ttttgctaca
     4621 ccttctacga tctttctttg acgctcttgt ctaccatagt caccatttgg atcttcataa
     4681 cgcatacgag aataagataa tgcctgatcc ccgttcaatg taattttacc gattggaaag
     4741 tcatagccat cttgtgagaa agtgaggtca ttattgactt cgataccacc aactgcatca
     4801 acgagttcct ttagcccttt catattgata gagacatagt gatcgattgg gatgtctaca
     4861 tatttttgta gggtatccat cgccatggca gctccgccga atgcgtaagc gtggttgatc
     4921 ttgtcttttg tgtcacgacc tacgatgtcg acatacgtat ctcgaggaat gctgacgatc
     4981 gttgtctgtt tgtcttcagg actgactgtg gcaaccatca tcgtatctga tcttccttga
     5041 tcgacccgac ctaaatctcc agtatcaatg cccatcaata gaacagagaa agattcttgt
     5101 ttgtccagat caactggttt cgttcgttct ccttgggttc gttccactga ttcgtaagtt
     5161 ttctcaatag aatgtgagag atccagatat aattttgctc ctactcctac aattgctaat
     5221 actaatacga taagaactgc taaaataatc agaaatattt ttttccctct tgattttctt
     5281 cttcgtcttg ccatagtttc ctccgtttgc taaccttgct tgaatcaacg ttttaactgt
     5341 caataactta tattgctata gcccatttgc tgagcaacag tttgtgcttt tgttgcgtat
     5401 gctgcgctta ttttttcatt ccggatctgc tctatttgac tgagcgccgt caaatattga
     5461 tcgtaagtag cggtggcagt cacttgacca tccttcaaca tcgtatcgaa taactgatct
     5521 agttgcgtga cttgcgcgat ctgagtatca gcgaatccta gatattgcat aacaaccgtt
     5581 ttccaaggac tatctttaaa aaagctcata ttttcacgta cacgagcgac atctgcactt
     5641 gtcacctttt ctttgatgac gacatcgtct acagcttgct gccagtttga tacattttta
     5701 gtaaataaat cgttgacagc tgtctggatc ttcagttttg attctgcact atctaactgt
     5761 gtcacgattg ctttcttctc atcttggatt gcaacagctt ctttaggcaa ttctgattct
     5821 ttgatgtcgt aatcatctgc tgaaagtttg acagaggata gcttatttga caaacttgtg
     5881 attgtttctt ctgttatttc agggtttaga aaattttttt cttttgtact atacagcttg
     5941 tctacttctt ttgataaaga agtcaaggtg tctttttgat tggtgatggc agattctgca
     6001 taattgatcc gttgctgatg ttgccgctga ttattgatat ggtaaccaaa caaacagatc
     6061 gcaatagcac ctgcacctac cgccacagca gtcaatgact tgacgaatgg cttttccata
     6121 gattcactcc taagtattga tgatagataa attaagatat aattcacata cataaaaatg
     6181 acagcttttt attttaaaaa ac
//
LOCUS       DAYYAR010000136         6174 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000136 DAYYAR010000000
VERSION     DAYYAR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.138"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHAT0_002461"
     CDS             725..811
                     /locus_tag="ACHAT0_002461"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8452366.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1622)
                     /locus_tag="ACHAT0_002462"
                     /pseudo
     CDS             complement(932..1622)
                     /locus_tag="ACHAT0_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1867..2781)
                     /locus_tag="ACHAT0_002463"
     CDS             complement(1867..2781)
                     /locus_tag="ACHAT0_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8452367.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2763..3143)
                     /locus_tag="ACHAT0_002464"
     CDS             complement(2763..3143)
                     /locus_tag="ACHAT0_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8452368.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3593..3880)
                     /locus_tag="ACHAT0_002465"
     CDS             complement(3593..3880)
                     /locus_tag="ACHAT0_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8452369.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3900..4124)
                     /locus_tag="ACHAT0_002466"
     CDS             complement(3900..4124)
                     /locus_tag="ACHAT0_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8452370.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4579..4749)
                     /locus_tag="ACHAT0_002467"
     CDS             complement(4579..4749)
                     /locus_tag="ACHAT0_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452371.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4764..5297)
                     /locus_tag="ACHAT0_002468"
     CDS             complement(4764..5297)
                     /locus_tag="ACHAT0_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8452372.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5318..>6174)
                     /locus_tag="ACHAT0_002469"
     CDS             complement(5318..>6174)
                     /locus_tag="ACHAT0_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8452373.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1634 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa atataacccc tggcaataaa
     1501 aacctcttaa atcgaatttt agagcctttt aatgaatatc ttgtttgata agccttatag
     1561 ctaagccaaa taattcctaa aatactggca aaaatgagta gatagggtaa atagaatgcc
     1621 atttctattt attctcccca taccagccaa aaggctaaca gtgtcatgat taagcaacat
     1681 aagggggcaa gaatatccat tgatcgctgc ataattactt ttttctgttc tttatttcgc
     1741 tggtttaata gcgcatggtg gaattttttg acttgtgaat caattgtagt ttgtattctt
     1801 tctttggtat aaggtgcaag aaaagcacgt tgtttcttta tttcattttc tatgtcttgt
     1861 aacgttttag aacgataatt cttgttggta gtctgctcct ctttcaacgt gttggtcatg
     1921 gtttcttcga gtcggtctaa tttccggttg ttcagtcgta agagttcgtt gttctcttcg
     1981 tacttctttt gcagtttcgc gaatgctagc tcgagctctt cgtagctcgt tggttcgttg
     2041 ttgattgtag gctcgttctc gtctgtccag ctcatgtaaa atcgtctcct tttcgtaatc
     2101 ctttcctagt ttgttggatc gtactttttt ctttttgtgg atcaattcgt aagtaaaatt
     2161 tttcccacga ttcaagcaat gaatgccgaa tgttttaaga gaatcacaaa attggttata
     2221 cgttcgagtt tttttgtttt tcattgtttg atcaatcgca aaccgaattt cgtctttcca
     2281 aacaggttca tttttttctt ttaatttgat ttcagacatg gttcgttttt cgggtttctt
     2341 ccctcgttca gtgattgtta agtcatgttc aagacaaatc tcgtcattta ggtcataaca
     2401 tttgtttaaa aatgcttgac catgggcttg aaatttgttc ccagtttcca gattgacgga
     2461 gttaatgaca atgtggttat gaatatggtc tttgtctgta tgtgtgtaaa tcgcgatttg
     2521 gtggtcaggg gctatttttt tcgcatactc tgcccctagt tgattggctt tttcagcggt
     2581 aatttctcct ggtcgaaaag actggatcaa taagtgtgct tgtacacgat cgtcttttcc
     2641 atacagcatg cgaatttgtt tcatttcgct tttggcataa tcaatgtcac agtttatccc
     2701 tgttttgacg gttgctcgtg gttcggcgta attgatcgct cggctacaag aagtggttcg
     2761 gcttacttta actgttgcca tatcttttgt agctcctttt gcatgatttt taggttttcg
     2821 gataagcgtt gggcgtcgcc aggatcaacg tcgtgttggt gtgtgttgca ccacttggca
     2881 atttgattga tattcccgcc agctttggct aaatcgcgcg cgattgcctg agcaatgtct
     2941 ggcgccactt tcggtgtcac cagacgcgcg ttttgtgcct tggctttcac gaaagccggc
     3001 acactcattt gcaaagcttc agctgactgc ttcagctttg caaattcgga ttcgctcact
     3061 cgaaaactga tttgttttgg ctcggatcga taggtatatt ttttagaatg attgctagcc
     3121 aaatattgat tttgttcgct cataagtccc accctttcag tacgtcctac ggacgtatcg
     3181 ttcgcaatac cattgtacct aaaaaggtac aataagtaaa gctcactaaa gctcactaaa
     3241 gggcttggca agctctatat ttgctagcca aatacctacg tatttggtgc aaataaccgc
     3301 ttgtcaaagg ggcgcaagct cccccttgaa tccccctccc ccccactctc cacaatacaa
     3361 aaaagactag caaatgctag ccactttttg taaagtttcg cttgtttcac cttcggtgaa
     3421 attgttgcaa atcaataatt aggtagagct acattttttt cgttgttcaa agattcttat
     3481 gccatttact attaataaat ctataaatat gataattttt cggctctata atttatggga
     3541 ctgatgaatc agaattgatt cttcagtccc attttcattt tattccaaag aattaactcc
     3601 aaataccaat agaagcccat cctctatata cttgagattc cttactaaca atttcttgta
     3661 attctattaa agatttaggt aatccaaatt tatgttttaa aatatatctt ccaagaaaac
     3721 tacttaattt catactaact tcaccaacac tttttccctc actgatttct ttagaataaa
     3781 gaaagagctt ttcttttaag tcaggatagt catctatagt tgcttcattg tatactttac
     3841 taattgcatt taaaagtttt tcttctttag taaaatccat tataataacc tccctaccac
     3901 taccttctag gtgcccatgg accatgatta acccaaccat taacaataat ttttccaatt
     3961 tctcctttag cttgattcca atctacccaa catttttctt tgttacaata aagaccattt
     4021 ccatagtaag tagctgcatc aacttttatt cctgtaccga tgccagctag acaagttcct
     4081 aatattccta gaataacaca atgttttaat actttctttt tcatgattgt ttttcctttt
     4141 cgttttttta aaattgcttg ttatattaaa taatcttagt aattttatta tataacattt
     4201 tattatataa caatgattaa cgtcataaaa agtagttttg aattcataat cgtttaaata
     4261 aaattataag atcagttatt taaacagcta gtagacataa tgtgtatact accaagtaac
     4321 gcctgtacca agggatccag gcgctgattt tgtgaatttt tattggtcta ggttaaaagc
     4381 tctttttaag gcttggtttt ctgcggttgt ttctttctaa ttctttgtga atattgatcg
     4441 gtcatttttt aaatacttgt tatttttgtt tgtgtttgtt atgcttttta catatctcat
     4501 attcatttat tattcttttc taaggttaat aataggataa tctctttcag aaagactgct
     4561 cgtacgagca gtcttttctt atttatcaag tttttctaca aatacttcct cattttgact
     4621 gttgtagtaa ttaggattga gttttagttc ccctttttta tttcctttgt tggccactga
     4681 taataacatt gggctaaagc ttaatattcc tagtaggata gaaccgaata gcaagtgttt
     4741 ttttgtcata agtatttcct tcttcatttc catagctgcc accatttttt ccaattcttt
     4801 gtttgttcgt ccattgtttc tgttgcttca gaaagagtcg ttgaactatt ctctgaagac
     4861 tcttccgtat aagtcaactg cagttgctcc tgaagttttt cgatttgttg gttggcttgc
     4921 agggtgagtt gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctttg
     4981 agggcaagtt gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt
     5041 gcaaccacgt ttttgtttgg tatataagca tttttatcgt tgttttgttc atgtttatgc
     5101 aacttatcta gcaacctttg attgtctaag ttgcgagcaa gttgcaatac tccagaacgc
     5161 aatacaacct tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata
     5221 tgtttcgata ttgcttgcct actcatctta aaaagatctg acaattcctt tattgtaaag
     5281 gtttcttgtg gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat
     5341 cagataataa actttttttc tctttttccg aaagtgcgga ctctgtttgt ttttcaagag
     5401 cttttttctc tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct
     5461 gtttgatttt ttcgttaatt gttgtagcgg gttcttcgta ttctttttgt ttatcagcta
     5521 aagccaatgc tttattttgg tcttctttgg ttggtgcatg tttgttaaag tcgtctgctt
     5581 ttttgatctc aggtttgaac gtaaattgat agccaataac aggttttccc cgtccttttc
     5641 cgtatttctt tttgattgtt agtcctttga agattgcagt tagttcttga cgtataggtt
     5701 ttataactcg tttatctata tcaggttgct tataactttt aggaatatct aataattcaa
     5761 gaaagtcttc tttggagaaa tatgcgaaac ctttggttct gtattgcttt aacagacgaa
     5821 acatagtctt tgcatagcca ctttgtagct cgttgaattg ttgtaaagaa tagcgtaccc
     5881 actcgtctaa attatttaac aaaggcagtg ctttttcgta aattcgtata tctacgtaag
     5941 gattttctac gtccccgttg attttaaatt ctgtaaacat tacaaacata tcgtaagaaa
     6001 gcccagactc acttctacga ccaaagcgta aagataagat tttttcataa gttgctttta
     6061 aatcttctac aaatcgtaca tttgcagttg ctttgtagtt acttaaatct tttagttggt
     6121 caaacgtaaa acgaactgtc ttatcccctt tttcacgcat tctagacacg atgg
//
LOCUS       DAYYAR010000137         6154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000137 DAYYAR010000000
VERSION     DAYYAR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.139"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..1099)
                     /locus_tag="ACHAT0_002470"
     CDS             complement(137..1099)
                     /locus_tag="ACHAT0_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8452374.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1101..2540)
                     /locus_tag="ACHAT0_002471"
     CDS             complement(1101..2540)
                     /locus_tag="ACHAT0_002471"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8452375.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2725..4671
                     /locus_tag="ACHAT0_002472"
     CDS             2725..4671
                     /locus_tag="ACHAT0_002472"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8452376.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4935..5807
                     /gene="scrK"
                     /locus_tag="ACHAT0_002473"
     CDS             4935..5807
                     /gene="scrK"
                     /locus_tag="ACHAT0_002473"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8452377.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1958 a         1051 c         1093 g         2052 t
ORIGIN      
        1 atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt
       61 tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta
      121 aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat
      181 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
      241 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca atgaaagagg
      301 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      361 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      421 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      481 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      541 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      601 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      661 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      721 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      781 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      841 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      901 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
      961 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
     1021 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
     1081 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
     1141 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1201 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1261 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1321 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1381 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1441 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1501 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1561 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1621 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1681 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1741 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1801 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1861 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1921 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     1981 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     2041 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     2101 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     2161 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2221 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2281 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2341 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2401 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2461 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2521 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2581 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2641 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2701 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2761 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2821 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc
     2881 acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     2941 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     3001 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     3061 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     3121 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3181 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3241 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3301 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3361 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3421 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3481 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3541 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3601 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3661 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3721 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3781 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3841 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3901 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     3961 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     4021 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     4081 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     4141 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4201 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4261 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4321 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4381 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4441 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4501 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4561 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4621 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4681 cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat caaatatttg
     4741 agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta ttagaatttt
     4801 aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca tgttactaaa
     4861 taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat tctaaaataa
     4921 agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg
     4981 ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg
     5041 aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggga
     5101 ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca
     5161 ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc atgaaaaaag
     5221 aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat ggtgagtatg
     5281 tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt acgggtgttg
     5341 gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca gaaatggggc
     5401 atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca tttcatggca
     5461 actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg aaaaaaggac
     5521 aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt gctcaatgtg
     5581 cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt ggtgtgatga
     5641 aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc aatgggtatg
     5701 tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac aacgctggaa
     5761 cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg aaaaaaggct
     5821 cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat aaccagttag
     5881 gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat gagataaatt
     5941 cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt gcaatagttt
     6001 tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat aggttctgtt
     6061 gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata gcaggaggaa
     6121 tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DAYYAR010000138         6146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000138 DAYYAR010000000
VERSION     DAYYAR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.140"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..537
                     /locus_tag="ACHAT0_002474"
     CDS             181..537
                     /locus_tag="ACHAT0_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8452378.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            547..1446
                     /locus_tag="ACHAT0_002475"
     CDS             547..1446
                     /locus_tag="ACHAT0_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8452379.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            1966..2694
                     /locus_tag="ACHAT0_002476"
     CDS             1966..2694
                     /locus_tag="ACHAT0_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8452380.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2839..4866
                     /locus_tag="ACHAT0_002477"
     CDS             2839..4866
                     /locus_tag="ACHAT0_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8452381.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            4974..6071
                     /locus_tag="ACHAT0_002478"
     CDS             4974..6071
                     /locus_tag="ACHAT0_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8452382.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         2132 a         1011 c         1266 g         1737 t
ORIGIN      
        1 tttgggtctt tttccgttca agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg
       61 ataagagaaa tcataataaa cgaatgactt actttccaat aaattgagaa cagtcagaac
      121 cagacaaatt agttgcatct gatgaataaa gtgattattc tatgacatga ggtgatacag
      181 atgaaattag aagtgacaaa agaagcacaa gaagtattga agaataaatt agaagcagac
      241 gatcaacttt tattagatat cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt
      301 tgccaattgg atacggcatt tcgattgatc attgtcaaaa aagatacaca aacagatcta
      361 agtctttatt ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa agacagtgca
      421 atactttaca tggatgatcc atccacactt gctttagaac caacttataa tagtttacaa
      481 ttaaaaggac caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa agactagagg
      541 gataaaatgg gaaaattggc gatcatcagc gacctacatg tggacatcaa taaattcggc
      601 gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat
      661 ctagccggag atacggctaa taaagtagac cgttgcctag ctgttgtcga atttttcgtc
      721 aataaaggat tatctacgac atttcatttc ggcaatcacg agttggcaga tgtgcccaat
      781 gaacagatga tggaccattt tccagaccca cattttttaa atgaacggta tattgcttta
      841 aatgagcaga ccgttctttt agggatgaat ggctggtacg actatcagtt ttccgaattg
      901 aaagattacg aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg gttcatccag
      961 cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta
     1021 gaccagctgg aagcaaaaaa acttgaggtg attttatcca ctcatttcgt tcctaaacgg
     1081 gcatttatca tgtatcaatc tgctccttat gagcgttgga acaaattaaa tgcattttta
     1141 ggatcagaat cattcggaga attacttgac cgttattcaa atatcaaaca agtagttttt
     1201 ggccatacgc atcgtcgctt tgaagataaa tccatcggtc acacgattta cagctgcaga
     1261 ccttttggtt attattatga atggcagctg acccgagatt ttgtcttgaa agaacagttg
     1321 ctagacagct atgatcctat gaaattacgt gtcctcttgc gtgcccatca cccagctttt
     1381 caggcctatc aaacacaaca tttgcaagag gaatttgaac aagcgatgac aatcgttccg
     1441 tattgagaca gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata aggataagta
     1501 agagcagtag atccatttat cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa
     1561 tgtttatttg atcggatgat ttgtaactcg cagagtacta gatgcaagcg tgtattcttt
     1621 ttttgaacga taatcacaaa aaaaaatata atatacattt tttatttaat tataattaat
     1681 attgattgaa gtctaggcag tcaagtcaca ttttgataga taggaggggg acttacgaat
     1741 tattttaaag gctatgacag ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa
     1801 actagaaatc aaatcgggct gataatcatt tataactaat cgatacatag gaccaaatat
     1861 aggtctatgt tcttttattt cataagtatc gtaaaacaga attttgctat ggattaatcc
     1921 aatcgactga tatgtttagt aaataaagaa agaggaatgt caatgatgaa aaaagaaatc
     1981 gtattaacag cagcaatgat gttattcagc atggtagcaa gtacgacttt tgtttctgca
     2041 actgaagtat acccaaagga atataacaca gaaggaacga ttacatttga agcaggagat
     2101 gagggtgtaa ctcctcctgt tgatccagag aatcctgatc ccaataaacc tgtcgatcca
     2161 tcagatcctc caagccctgg aacaggaggg gcattatcga ttgattatgg atcaaaattc
     2221 aaatttggga ctcaaaaaat ttctactgcg gataaaacgt attatgcagc tgctgatgtg
     2281 atgaacgatg ggtctagaaa accgacatat gtccaagtga cagataggag aagtacctta
     2341 tctgggtgga aattaagtgt ctctcaacca gaacagttta aaactgccag tggggatgaa
     2401 ctagttggag cacagctgaa atttacgaaa gggcaggctg tttctttggt tgatccaaca
     2461 tatacgccgc aaacggtgaa ttctgaatta acgttgacac cgggaggaaa caatacgtta
     2521 gcaattaatg ctaaatcagg aacaggtgtg gggacttggg tctatcgatt tggtgcaaat
     2581 gaaaatgaaa atcaagatgc ggtacagctt tcggtacccg gtaaatctgt caagttagcg
     2641 caacaatatt ccactaaatt agtttggaca ttagaagata cacctaacaa ctagtttact
     2701 gaaaagaact gttaaaaata aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa
     2761 attgtcaaag gctaggctaa atttgtttgg atacgaatag ttcatctagg aatttaacga
     2821 tcaggaggaa aagatatatt gaaaaagagg aggatcgtgc tcattttaag cagtctctta
     2881 gtgactaata tctttccgat catagggtac tctttggaat tgaatcagtt ggagcatgga
     2941 aatagaagag acgaagaaag caaactcact tcgacattcg acagtttaat agaatccaac
     3001 cagacacaag aaagcagtga ggatttaagt atttcgagta gtggagagac cactgacaac
     3061 gtagaagctg agggttcgga caaacaagga ctagaagatg caccaatttc tgcggttgag
     3121 ctgcctgacg atcccaatat tatttctatc gataaaattt ttcaaacacc tatcggtact
     3181 agcacgtcca tattggaaaa tgggaagtta ctgcagttga acccagcaga aaggtctcaa
     3241 agaggagcta tttggtcaaa taagaagatt agtttactct cggattttac atttaaatct
     3301 tatttatact taggaaatca acgagataga gcgggagatg gcatgacctt tacattgact
     3361 ggcgatccta gaatggctag taatcccagt caagttattg gatcgccagg tatgggaatt
     3421 ggctcatatt ctactcgttc tggccaacct tacattgcga atgcgctctc gattgagttt
     3481 gatacgtata aaaatagtgg ttcttctgat cgaatggatc gagagataac tgctgataat
     3541 agagggcatg gacatgtcgc ttttgtcacg cctaaaccaa ataataataa ttattctgga
     3601 gagcatactg gcgtgacggt agctcctaca tttctttcta atggcacatg gcgcttgtta
     3661 ataattaaat gggacgcgga gcaaagaagt cttacttatg atttggaagg tgtcggtcgc
     3721 tcttctcgga ttatacctga tctcattagg cagtttggtt ctacagaagt aaattggggg
     3781 tttacttctt caacaggtac tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca
     3841 tcgaatgtca catcaacagc tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct
     3901 gaagctggag gaaacgataa gataactatc agaaatacat tgaaggtcga gggggatttt
     3961 cttgaatata agaaaatgca gactagtatc gacctcccac aagaattaac tattcccgag
     4021 catatcttga tcaatggaaa tcaaattgat gccaaagata tcttgataac cggaaatcga
     4081 gtcacgctaa atctgacaga ttattttatt gctggagata cgttaataat cgaattggag
     4141 acaactttgc aggaagtaat tcctgaaaaa gtactcacta tgcattttga ataccttgaa
     4201 aacgggaatt tgattgagca atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag
     4261 atagatatcc atgtttatta taaagataaa gacagtcaga ttgatattgc tgaaatgaaa
     4321 acaatcactg ggaaaatagg agagaagtat aaagaaactc ctattgatat tgaagggtat
     4381 gtttttgact cggattcagc gaatacagaa ggcatcttct ccgaaaatac agaggatatc
     4441 tatttttatt acagaaaagg caaactttat ctctcaactg ctcctcaata tgtcaatttt
     4501 ggaaaacata aaatatccag tagaccatta agtgaattcg gacagttttc aggtgggttg
     4561 aaaattatag atgaacgggc ttttggaagt tggaaattac aattgaaaca agagaaagtt
     4621 cttacaaatg gagattttga gcttccagaa gctctttcct ttgttacctc tgaaaataca
     4681 acggttatag ggacatccgc tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat
     4741 ttgtcctctc ttttagattc tacagctcat cgtggaatca gaataaatat tcctgtagaa
     4801 aaccaaagaa tcggaacctt tgaggggaaa ctatcttgga tacttgcaga tgtcccagga
     4861 aattaacagg aagagaagaa tataacaaaa ttattgagtt tcaagaaggg cacaggtacc
     4921 ataggctctt gtgtcccaca gagacaagat tttgttatag tcttctctta catatgatta
     4981 aaggagcgat tttcttgaga ccaaaacaac aattatttat aacactccta gttattttag
     5041 gggcactttt taacaatata tcagtgtcag ctagtgcatt taatttttca gtgacgccga
     5101 taacttcaga gaatcaaatc gacaagagaa aaacttactt cgatcttcaa cttgatccgg
     5161 atcaggaagt agaagtaaaa gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg
     5221 atatttctgt gaatagcgca accaccaatt caaatgtgat ggttgaatac ggaaaaaacg
     5281 agattgaaaa agacatgagc ctgatctttg atctggtaga ttatgtgagc tatccacaga
     5341 tagtgacgct aaaacctaaa agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg
     5401 agcgatttga tggtgtattg gctggtggga tcacattcca agaaatccag acggaaaagg
     5461 accaaacaga aactaaggat caaagcttat cgattgagaa tgaatacacc tatattattg
     5521 ctttgttgat gcaacagaca ctgaacgaag ttgctccgaa ccttactctt catgaagtta
     5581 aaccagatca aatcaatgca agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa
     5641 cctatatcaa tcaagtagtt attgaaacaa aaattacaaa aaaaggacat tctgaagttt
     5701 tatatcaaga ggaaaaagaa ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa
     5761 ctgctttaaa tggtcaaccg ctaaccccag gtgaatatca ccttacaatg actgttttag
     5821 gaaatgaaaa tgaaaatgga aaattttcga gaaaaaaagg aaatactacg atcaactata
     5881 ccaatcaatg ggtatttgaa aaagatttta caatagacgg aaaagtagcc aaggaattga
     5941 acacgacgga tgttacgatt aaaaaagaca ataattggat ttatatctta gtagcttcac
     6001 ttttcttatt gtttatttta ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga
     6061 aagaaaagta aggttaagat aacaaatagt aaatttttat tattgtagga tggttgggct
     6121 ctttttataa caatataaaa tatacc
//
LOCUS       DAYYAR010000139         6107 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000139 DAYYAR010000000
VERSION     DAYYAR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.141"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1220
                     /locus_tag="ACHAT0_002479"
     CDS             105..1220
                     /locus_tag="ACHAT0_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452383.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1246..1686)
                     /locus_tag="ACHAT0_002480"
     CDS             complement(1246..1686)
                     /locus_tag="ACHAT0_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8452384.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1846..2352
                     /locus_tag="ACHAT0_002481"
     CDS             1846..2352
                     /locus_tag="ACHAT0_002481"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8452385.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2520..4433
                     /locus_tag="ACHAT0_002482"
     CDS             2520..4433
                     /locus_tag="ACHAT0_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8452386.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4438..5439
                     /locus_tag="ACHAT0_002483"
     CDS             4438..5439
                     /locus_tag="ACHAT0_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8452387.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5641..5847)
                     /locus_tag="ACHAT0_002484"
     CDS             complement(5641..5847)
                     /locus_tag="ACHAT0_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8452388.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2258 a          933 c         1213 g         1703 t
ORIGIN      
        1 gattcacttt acaaaggtac ttatcaatcc ttagaaaatc ttagtcaaaa aattatataa
       61 gtttgcgtac gattcaaaga aaattaagag aacttaatac tattttgaaa aactatcatt
      121 tatcttgcag cttgaaaaaa ggtaatccga tagaaggaga agaataccgt atccgatact
      181 tttttcactt gatgtattgg cagtcattcg acgaaaatca ttctgatata ttatcaagta
      241 taaaaaataa acgtcaacta ttagaaaaat ttgcgaaaca tgcgccatat acgcgtagta
      301 tagatagaaa gaaatggatc agcctactaa gaatttcgtt gcaacgaatg aaacaaggcc
      361 actcgattac gtatgtccca gaagagatga acaaatttgt gcatccattg ataacaaaag
      421 aagagtttac gcattacata ctagagcctt tttttgaagc aaacttctgg tcgatgaatc
      481 acatcactcc aacagagcaa gcttaccttt attttatgtt tggtgtaatg aacacctatt
      541 tggaagaaga tttgttggat actccagata aagtacaaga aagttggggc tggattgaat
      601 cgtctattgt agaaaatttt gaaaaaaact tcaagatttg ttttacaact tcagaaaaaa
      661 attatctata tgtcaatttg gcaatgatcc atttgtattc aagggttttt gggacaaaaa
      721 agaaggtgga tgcttttgga aaatcagcag aagacagaga ttttcagcat atatttccag
      781 tgatgtatcc aatcatgcaa cagtttttta gtggggtagc tcgcagaatt ccagagctta
      841 atacttatta tgaaaagaac agacggttga tttttcaata ctgtatgtta gtcagggata
      901 tttttataaa acacgaacaa cctgtcatat tctatattca atcaaagtat ggtaaaacac
      961 aggaattatg ggcaaagaaa aggatattat ctatgactga acgcccaata aaatatagtg
     1021 catccgctga tcagttacct gatattgtca ttagcgatta tcctatcgac aaaaaggcat
     1081 atgatcaaac gaatacacag atattttact ggaatggaca acctacaaaa aatgattggg
     1141 cgcgtctgaa agctgaaatc ataaaaatca ggaatgagaa aaatagatcc atattggaaa
     1201 aattggattt cgttaattaa gaaaagcaga ttgtttatgc caaaattaag acattgcaaa
     1261 aagattattt tctttaaaat cgacaagatg accagaaagc aagagttcgt tgagctcttg
     1321 ctttctttta gttttagaaa ggaacatcct gattttatct tcgctaaatc cagtcaaaag
     1381 ttttgcagat tggtcgtaga taatcggttt tttcaataaa gaaggggatg tacgaatctc
     1441 aatatatagt tcactcaaac tgagttcatc tattctgtga acaagatttt tataactaat
     1501 agaatgagta gagagcattt cggtcacgcc gttgtttgtc aattttagta agtggataaa
     1561 ttcatttttg gttagtggtt gacgaattaa gttctgttcg ataaacggta tatcatggac
     1621 gataagccaa cgttttgcgc gtcttgaaga cgagcagcca gaacctgtat acaatttaat
     1681 catcatacat tcctccaata gctaagtatt tatgaaataa taacatgaag aaatagtcga
     1741 aaagtgccat ttagaggtgt aaaataagaa aatgttgtca cgtataatgg aactagctag
     1801 ataagaaata ctattaaatc aagaaaaaag aaagaaggca caagtatgtc aaacacagcc
     1861 gacgcaatag tagtgatcga tttacaaaaa ggggtatgtt ttgattcttc gccgatacac
     1921 gaattagaaa cagtgacagc attagtgaat aaaagaatca aaacatacga aacgattggg
     1981 aaaccagtta ttttcgttca acacgaagat gctcaattag tgccacaaac aaaaccatgg
     2041 gagctattgt cggaattatc tattcctgag cacagttatt ttgtgagaaa aacccatgcg
     2101 aattcatttt ataagactaa tttgagtgtt ttattaactg aattggactg tcattcgatc
     2161 gaattatgtg gggcacaagc agagtattgt atagatacga ctgtgaagtt tgctcatgga
     2221 ttaggatacc agcttatgat gtgtagagga gcaacgacaa cttatgataa tccttttatg
     2281 aaagcaaagg aaacaatcgc tttttacgag aaaatttggg ataaacgatt tctgacattt
     2341 ttggagcaat aagttgtttc ttcgtttaaa aaaagcagaa tggatactgg tttatccatt
     2401 ctgctttatt acgtaaaatg agaaaataat cctgttctat ttttatggga taaaccattt
     2461 attttttgta taaaaaatgt ttgcgtttac atataattct cctagaaaca taaaaaggag
     2521 tgaatagctt gagaacaaaa caagtattaa agggagcgtt ggtcatctta tttgctgtaa
     2581 gtacaagcgt cccgcaatgg acagtatatg gagagagcat ttcttccgaa tcggaatctc
     2641 gggaggtaga aaccacagaa actactgatg ctggaaatga aacgaaatcg agtacttcac
     2701 aaacagaaac aaatactgaa acaacggttg agacatctgt tactagcaca gatcaagaaa
     2761 cgaccgctac cacagataac caacaaacag aagaagcaac agctacaaaa atgtcaaacg
     2821 acgaatcctc ttctgctaaa gtaagtgaca aagagaatgg gacagaaaat gatgatatcg
     2881 gtaaaggtga aaatttcaat ggtaaagaat atttgaatta cttcggtaaa cagatgacag
     2941 aaatacttaa tggaccaaca gaagacatca agtcaaaatt aatagagtta ttagtcactg
     3001 agcttgctgt taatgattat ggtgtagata aagagattat caaacatctg attgctactt
     3061 atctattgta cgatatcgct tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta
     3121 cagatgatgg gacaacgcct acagaaaaca caggcttcta tccaaataca aaacaacttc
     3181 atcaaactgt actaaacaaa caagatggca aaatgatgga tgtatatgca tattatgtcg
     3241 atcaaggttc agataaaaca gtcatgatcc atggcggatt tcgagggaat tggaataacg
     3301 ggatcgttac agaagaatac aatgattttt ataaggccgg gtataattta ttatttgttg
     3361 attccagagc gactggtaat agcggaggcg attatgtgac ctatggacaa tatgagtcag
     3421 atgacgtcct ttactggata aatcaagaag ttcgagagcg gccatctcaa aagattttac
     3481 tttatggtgg ttcgatggga gcagctacga tgatgtctgt attagcgaaa gatattcctg
     3541 tcaatgtcaa aggaattatt gaaaattgcg gatttgcatc aattgatgaa caactacgtt
     3601 ttacgtacag ccagacagta gcccccgcac taccggatgc cataaaaaat cagttagaca
     3661 tcattgggga tcaagagcat gaagatttat ttatgggcct tttgaaacaa tactattttg
     3721 atcaggagat gcatttggat acaaaagctg ctctacctac gattggaatg tcagattcat
     3781 tgcctaaatt gattatccat ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa
     3841 aattatatga gttatcagga gggtataaag atcttttatt agtagaagga gcagggcatg
     3901 gaaaggcaca ggaagttgac catgctgcct acacgaaaca tgtaacggac tttttgaaag
     3961 tagtgttcca tgatcagata aacgtaaagt atgtggatga aaacaataaa agtctactca
     4021 aagaccagga cgaaatccag ctttacggag cttatggaga aaattatatg acagagcaaa
     4081 agacattcga aggatacgaa ttagcaaatg ttgaaggacc gactgaaggg atttttaatg
     4141 agacgatacc aactattatt ttctattaca aaaaaatccc agtagctcca ccaaaaaagc
     4201 aagatccgac agagaatgca gacaataagg gaaagaatga acctcgagag gatcaatcaa
     4261 gtgatgatac gcaaccgatt cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa
     4321 aaagaatgtt gccgaaaact ggagaaaaga aacattcgct tctcatttat tcaggtggat
     4381 ttttatctgt tagcacgatt atcacatgga tttggcgaag aaagagaggg taaatgaatg
     4441 aaatggttca agcgaatagt gattgcagta ccagttatca tcgtagttat tttagtcact
     4501 gtatttttga ttaatcaaat cacaccaaag ccgattagct tgcttgttag aaaacaattt
     4561 gacacaagtg aaaaagaaca agtatatgcc cgaccagatg atttccagca aaaaatcgaa
     4621 caaatcgata taaaaaaaga aatatcctat ccttcaaagt acaaaaataa taatatggat
     4681 atctacacac cgaaagtgga aaagaaaaaa ttaccgattc ttttctggat gcatggaggc
     4741 gcatatgtag gaggggataa aaatgattgt cgtgattatt tggaatatct atgttcagac
     4801 acgcagcaaa tcatcgtaaa tatagattat gaacgatcac cggaagcaaa acatcctaca
     4861 ccagttatcc aattgaacga agccattcaa gcagctaaaa aagagataaa agatcaagca
     4921 gattggtcga atatttcgat tggtggaaat tcggcaggtg cacaaattag tggagaatat
     4981 ttgttagcac ttcaaaatga agaaattaaa aaagctgatc atcttgatcc gactttggag
     5041 aacaagcaga ttacgaagtt tatttctttg tctggtctgt tggatccttc tgcacttact
     5101 caagtaagtg ataaaatctc tagctttctt tatgaaaaat gtggatgggc ttactttgat
     5161 aataaggact ttgaacaatc agagaaggtc aaaagtttag ccttggtcag acatgcggat
     5221 cgttggacgc aaaacacatt cttaacggat ggaaatacaa atacgtttac gaaacaaatg
     5281 gaagaagtaa cttctgtatt tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg
     5341 gagaaaaatg ctgttttgaa ccatgaatat cagtttgatt tcagcaataa acaagcgcaa
     5401 gaaacatatc aaaagttagt aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg
     5461 ttgtaatctg tcacttagct ccaagtcaca aggagcaatt tcaataaaca agcttttcat
     5521 ggtttatgaa tattgtaact tgtgactgag gagttagaga tggcaagggg caccttttat
     5581 tcgaaatttg ggaatatcac acgattattg tcacgatctc ttttacgtaa attttataag
     5641 tcagatttgt tgttttgatc gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag
     5701 aagggacaaa aagccgagtg gtaagcaaaa gaatgctggt tccactaata ttccgttgtt
     5761 attgatataa ggctgggaac aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa
     5821 aagcaaagaa atatggatag ctttcataag cagcacctcc ttttttataa ttgtatgata
     5881 aatagattaa gaatctataa atgtgacaag tttgttagga tgtgaataaa atgatcatct
     5941 agcgaacttg aaacaaagat ggctaatgat aagcaattaa tgattcagca atcagatagg
     6001 tacaaagttt accatcatac gtctagacca ataatagaaa atatgggaat ataatagaaa
     6061 agatttagga tacccccaaa agaatttccc caaaaattcg atgtatc
//
LOCUS       DAYYAR010000140         6068 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000140 DAYYAR010000000
VERSION     DAYYAR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.142"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACHAT0_002485"
     CDS             832..1566
                     /locus_tag="ACHAT0_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8452389.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACHAT0_002486"
     CDS             1582..2751
                     /locus_tag="ACHAT0_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8452390.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACHAT0_002487"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACHAT0_002487"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8452391.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACHAT0_002488"
     CDS             3841..4767
                     /locus_tag="ACHAT0_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8452392.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACHAT0_002489"
     CDS             4786..6036
                     /locus_tag="ACHAT0_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8452393.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAYYAR010000141         5981 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000141 DAYYAR010000000
VERSION     DAYYAR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.143"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..907)
                     /locus_tag="ACHAT0_002490"
     CDS             complement(32..907)
                     /locus_tag="ACHAT0_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8452394.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1055..1387
                     /locus_tag="ACHAT0_002491"
     CDS             1055..1387
                     /locus_tag="ACHAT0_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8452395.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1499..2707
                     /locus_tag="ACHAT0_002492"
     CDS             1499..2707
                     /locus_tag="ACHAT0_002492"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8452396.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2861..3316
                     /locus_tag="ACHAT0_002493"
     CDS             2861..3316
                     /locus_tag="ACHAT0_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452397.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3678..3953
                     /locus_tag="ACHAT0_002494"
     CDS             3678..3953
                     /locus_tag="ACHAT0_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452398.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3966..>5981
                     /locus_tag="ACHAT0_002495"
     CDS             3966..>5981
                     /locus_tag="ACHAT0_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8452399.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPS"
BASE COUNT         1933 a         1013 c         1265 g         1770 t
ORIGIN      
        1 ctttcctcat agcctatctt tgtctttttt cttatagttt tttcagtaat cttgtctgtg
       61 gcttcaaata agtaaagcct tgcccttcca cttcatgcac attgatgatt gaaaggaaag
      121 ctttttcgtc aatctcgtgg acgatcctct tagcttcagt taattcacta gagctaacga
      181 ccatatagat gatttttttt gaattaccag agaatgcgcc ttctccagaa aggaaagtca
      241 cacctctttg cagaccggtc atcagtagtg gtgcaatctc ttcgcttttg ttggagacga
      301 caagtagacc ttttgcagaa tatccgccat ctaagatcga atcaatcaca cgactgaaaa
      361 catacgaaac aatcaacgta tacatcatac gtttcaagtc gatataggtt aatgaagcaa
      421 gtaagacgat gatatcaaat gccaataggg aacgccccat agtgatgccg taattttgtt
      481 ctaaaatccg agcgataata tcactgccgc ctgttgttcc acccatgcgg tagacgatcc
      541 cgcttcctac acctgcaact aaacccgcaa gtagagaaac gatcagtaga tcatgctgta
      601 aattgatttc tagtggaacc ctttgccaga tccataaaaa gaatgacaaa gaaagtgtac
      661 ctagaacagt ataggccaag gaacgtttac cgaggatctt tcctcctagt aaaatcaatg
      721 ggatgttgat taataaagtc gtataagctg gatcaatacc aaataaagct cttaagatca
      781 aggtgatccc tgagactccc ccttctgcta aatgattagc gatgttcaag taaacaagac
      841 caaatgcata aatacttgtt cctataatga taaaaagaac ttcttttact gtttttcgtt
      901 tgaccatatg aattccagct ttcaattatg tattttcaca gaatttccat ttagaaattc
      961 cttcctaaat gtagcatgga aattcgattt tctcaattgc tcttatctag gatttgcgtt
     1021 aaagttttgg taagatagtt ttggagggaa aaagatggat aaaaagcgtt ctttatacag
     1081 tatgcagcag gaagtggatg aatatatcca gcaattcaag gtggggtatt tctctccatt
     1141 agctcagatg gctcgattaa ctgaagaagt aggtgaattg gcaagagaag tcaaccacac
     1201 ttatggagaa aaaagcaaaa aggcttctga accagttaac tcggtagcag aagaacttgg
     1261 tgatgtttta ttcgttacga tgatcatggc taattcattg aatatcgact taactgaagt
     1321 ttttgaaaaa aacatggaaa aattcaatca aagagaccat aatcgctttg agcgtaagga
     1381 cgagtaaaca agatgaagtg gtcctggcct tgaagactct gaaacggact tgtcaaacag
     1441 tcaccatgca actgagtctt tatctcagga caagaaacac gcaggaaagg ttggaaaaat
     1501 gaaattaaaa caattacctc tggaatacca aaaggcaatt ccagttctga aaaaattaga
     1561 gaatgcagga tatgaagctt attttgtagg tggaagtgtt cgtgatatct tgctgaacca
     1621 gccaatccat gatgtggata tcgcaacaag cgcatttcca gaagaaatca aacagctttt
     1681 tccaaaaacg atagatattg ggatcgaaca tggaactgtg ttggtattag aaaatgatga
     1741 acaatatgag atcaccacat ttcggacgga atcaacttat caggactacc gtagaccaga
     1801 tcatgtcgaa ttcgttcgtt cgttggcaga agatttgaaa agaagagatt ttacgatcaa
     1861 tgcgtttgct ttgaaagaag atggagaaat catcgatttg ttcgatggtc tgcaagatct
     1921 agaagaacag atcttgcgag ctgtaggcaa tcctcatgag cggttccatg aagatgcctt
     1981 acgaatgatg cgcgggttgc gttttgtcag tcaactaggg ttcaaattag aggaagaaac
     2041 cttctacgcc atcaaagaaa accacgagct attaggtaaa atatctgtag aacgtataaa
     2101 tgtggaattc atcaagatgc tcttgggaaa tgaacgggcc gcgggaatcc atgcatttat
     2161 tgaaacagag tgctacattt gctgtcccgg tttgaaagaa gaaggcgaag cacttttacg
     2221 ttttgctgaa ttaccatccg tcccattgaa agaagagagc caagcatggg cattattgat
     2281 cagccaactg gatctaaaag aaaatgagat tcgttccttt ttgaaatcgt ggaagtgttc
     2341 taaccaaatg attaaagacg ttcaagcact tgtccaagga ctacgacaac gagaaactgg
     2401 agctttggaa ccattcgaat tgtacttctt aggcaaaaaa catgcactgc aagtagaaga
     2461 attgatgatc tattttgacg aaacacctca gatggatgcc gtaggagaat cctatgatcg
     2521 tttacctatc aagtcattaa gcgaattagc agtagatggt aaaatcttgc tagaagaaac
     2581 tggaaaaaaa cctggtaagt gggttagtga agctttgcaa ttagctgaaa aagccgtcgt
     2641 agaacaaaga ataaaaaatg ataaaaaaca ggttttggat tttttatcga aagaaaaatt
     2701 gatttaaaaa acactagact gtatctgctc agctttgttg gctacttaaa gtgggcagat
     2761 acgtcattag aagaaaaaac ataagatgtg accacttaca taaatttgtg ctaaactgag
     2821 gtagtacaag aataaattac atcataaagg agtcttttta atgagtaaag aaatgacagc
     2881 gctaaaattt tattttagaa atggagaaac ctggacgatc aaccgccgtc atatcggcga
     2941 tctttggatc aaacaaatca caacaagttt tggacgaatc aatggcagtg aatttgttga
     3001 aatccatccg tgtgctggat ttaaaatcga aatcttccat gaaggggacg cagttgcgac
     3061 acatgatatc aacctaggcg gactggaaat gggcatgttt aatcgcgcat tgaaatacga
     3121 agatatcgag cgaatggaaa tcctttaccg aaatggcaca cctgatcttg tgtacttccc
     3181 atatctagat aaaggaacag aaggattaga caatcaatat caatcaacaa aaatcagcga
     3241 aaaaacaggt aacttgtata tcgtgatcaa tccagatcaa cgtgtagaag atgtatacgg
     3301 ggaatttttc gaataaatcg atttttacgc aaaaatgagg ctgtgaaaga gatgtgtctt
     3361 attactactg ataggctgtc tcttccacac cgtttattca gaataaggga ctgtgacaag
     3421 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
     3481 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
     3541 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
     3601 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
     3661 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
     3721 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
     3781 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
     3841 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
     3901 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
     3961 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
     4021 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
     4081 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
     4141 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
     4201 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
     4261 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
     4321 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
     4381 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     4441 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     4501 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     4561 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     4621 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     4681 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     4741 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     4801 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     4861 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     4921 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     4981 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     5041 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     5101 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     5161 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     5221 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     5281 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     5341 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     5401 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     5461 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     5521 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     5581 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     5641 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     5701 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     5761 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc
     5821 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     5881 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     5941 atatcaattt gttagaatct tatacgatga acaaaccatc t
//
LOCUS       DAYYAR010000142         5935 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000142 DAYYAR010000000
VERSION     DAYYAR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.144"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..632)
                     /locus_tag="ACHAT0_002496"
     CDS             complement(147..632)
                     /locus_tag="ACHAT0_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452400.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(771..986)
                     /locus_tag="ACHAT0_002497"
     CDS             complement(771..986)
                     /locus_tag="ACHAT0_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8452401.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1210..2010
                     /locus_tag="ACHAT0_002498"
     CDS             1210..2010
                     /locus_tag="ACHAT0_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8452402.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2029..3897
                     /locus_tag="ACHAT0_002499"
     CDS             2029..3897
                     /locus_tag="ACHAT0_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8452403.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3890..4381
                     /locus_tag="ACHAT0_002500"
     CDS             3890..4381
                     /locus_tag="ACHAT0_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8452404.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4369..4923
                     /locus_tag="ACHAT0_002501"
     CDS             4369..4923
                     /locus_tag="ACHAT0_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8452405.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4941..>5935
                     /locus_tag="ACHAT0_002502"
     CDS             4941..>5935
                     /locus_tag="ACHAT0_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8452406.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2075 a          946 c         1199 g         1715 t
ORIGIN      
        1 gagaagtgag tatattcata agagaaatag ctgtaacgaa ttattaggtt aatgcaaatg
       61 cttatttata ctccgtataa ctttctcaga aaaaatgata ccaataatga cttgctagta
      121 ctgtaaatcc gtataatatc atctaatcaa aatggtgttc catttgcatc tgtcatcgta
      181 atcgatcctt cgggtgacac tcgataaaaa ccaacgcttc cagatcctcc ttgggaacgg
      241 atttgtttgc tggaagctgt gaacaaataa tctcgatcaa ttttttgata aaagctaata
      301 acaatatcgt cactttgtaa ttcggaacgg tgggcaataa gaaatagaag tgctttgtca
      361 gagttttcta atggaagaga aggtacagtg ctttcttgca tttcagatgt tgaagaaatg
      421 ctaaacgact gaatacttga ttggctatgt tgcgaatcta cattaccagt agaagtatta
      481 gtaatggaag tagtagaagt acttttagtt gaaccaacct cagttgttga aatggctgct
      541 gaagattctt taacagaatt ctttgtttta tatccacagg cagaaaaaag tattacagtt
      601 cccaacaagc ctgcgtaaat ccctttatgc atgatagctc ctcctaaaat agattatctt
      661 tattcagtat atcacttaaa caaagaaaag cagatggttt aaaatgaaaa aaccttccca
      721 ataaagggaa ggttcatgaa gataataact tttttaataa ttatagcttt ctaatatctt
      781 tcatcaactt ttctagtaat cattgggaaa aatttcaagc agtagatcgc acaaatacct
      841 acgacgatat aaatgatctt cgcaaaaata gttgttgaac caccagcaat catagctact
      901 aaatcaagtt cgaacaagcc taccaacagc cagttcaacc ctcccacaat caataaagtt
      961 aatgcaatag catctaaagt tttcattaga attctccttt cttaaatgat aaaacaatta
     1021 tatatcaaaa agagaaacat tgcttatata acgctcagga attttagcaa tctatgcaaa
     1081 aagatgaaaa ttttttttgc tcttccatta gtacacattt tttagctgaa gctgtaaatt
     1141 tcatgtctta atttttttta gtgagctttt atactggatt tgtaagaaaa aagaaggaga
     1201 ggatcacata tgtcggattg gttggatatt gcaggaaaag tagtaattgt aactggaggt
     1261 tcctcaggaa ttggaagaag tatcgtcgaa aatttgttga agcagaatgc acaagtagcg
     1321 aattttgatg taactgaatg ccgtatacag catgaaaacc tactatcttt aaaagtcgat
     1381 gtgtcttcta aaacggacat agaagaaggg atatataaag taatgaaaca tttcaagacg
     1441 atcgatggtc tagtcaataa tgcaggaata aatattcctt ctcttctaat agatagaaat
     1501 catccaaaaa gcaagtatga attatcagaa caagtttttg ataagatgat cgctgtcaat
     1561 caaaaaagtg tatatttgat gagccaagca gttggaagaa tcttggtgca aaagggatca
     1621 ggagtgatcg tgaatctttc ttctgaatca ggattggaag gatcagaagg acagagctgt
     1681 tatgctgcta caaaagcagc gatgaacagt tttactcgtt cttgggcaaa agagttagga
     1741 aagaggaatg ttcgtgtggt aggtgttgca ccagggatac tggaagaaac cggtttgaga
     1801 acacaagaat atgaagaagc tctttcttat acgagaggta taagcgtcga acagctgaga
     1861 aacggttaca gtcgaacaag tacgatccct ctaggaagga gtggtaagct gcaagaagtg
     1921 gcggatctag tctgctatta tctttcagaa agatcaagtt atatcactgg agtcacgact
     1981 aatatttcag gaggtaaaac aagaggataa aggatagggg ggacgcacgt gacgctagta
     2041 aacagatggt accaattatt gaaattacta gtcactcaca agaaagtttc aatccaggaa
     2101 gtgcaaaaag aaatcaaaac cagtaatcaa accataaaaa aagatattgg ggagttgaat
     2161 gaagaaattg ccggtattgc aaaaatcatc caaaaaaaca aacattttga acttgaaatt
     2221 tatgattttg atttttttga tgagatcatg caaggaaaat tgaaaacagc cagtgatttc
     2281 aattcggcat ccaaaagaat cgcttattta ctaaataggc tgatcgaaac agaggatttc
     2341 ttacgtatgg atgatttgtc tgaagaagtc ggtgtcagca gaggaacagt ggtcaaggat
     2401 ctaaagacat taaaagagat gatcaatgat tttgatgtaa agattacagg cacaccaaat
     2461 aaaggactaa gaataatagg agatgagcta gaattacgac tacttttatt ttattttgtt
     2521 tctccctact tttcagagac gaaaacagaa cagtttctag aagaaaaaca agcaatcaaa
     2581 cattttcaga agaagacgaa tgctagtaaa caagaaatca cccttttaac gaaagttatc
     2641 ggtatttctt tgaatagaat ctctagtaac tatctgttga agaagcccat caatcgttac
     2701 agtgggtgtt ttgaatatat ccaagaattc aatgaactga tcaatcagtt ggaagaaatc
     2761 tatgaactaa ctttgagcca atatgaaaaa gattttatca gttttccttt gaacatcatc
     2821 aacacaagta taacgatgat aaggaaagct aactctgatt tgcgcttata tttcgacccc
     2881 atgatgatgc gtattcgacg attagatctc attgatctgg atgagtcctt cttatatcaa
     2941 gagatgaaac agcacctctc tttattgata aatcgggtaa tttttcgtta tcgattgact
     3001 gatttatttt atggtgagat agaaaaaaag tatccttttt cttaccaaat cgctagcgaa
     3061 tgcatacaag cattagaagc tatcttgcaa cataagatct ctaaagtaga gatcagctac
     3121 ctaacgttgt atttggagtt agcatttcga tatcaaaaag agcatttttc ctctaaaaaa
     3181 gtagccattg ttcatacgac aggaaaagga acagcattaa tgatccaaag acaaatcaaa
     3241 aggatattgg ggagggatat acaaataaca catgtttcag aagaacaata tgaaaaagta
     3301 gatctaaaca aatatttcgc tgtttttaca acgattccgt taaaaaatgt aaatgaagag
     3361 acaccggtga tccatttgac taatcttttt gatgactctt ggttaagggc acaatggcaa
     3421 aaagcaaaaa aaaagcgatt agccgggttt cattctactg tttttttcta tcaaatatgg
     3481 aaagaaaaaa agacgtatca agaatattta aacaaattag tcgctccttt gatcagaggc
     3541 tattatgtgg atgaagaatt tgaaaaaagg atcttgaaaa gagaatatca gcaaagcaca
     3601 tttgttaaca aagaatttgc ttttcctcac acaaccaacc agctatctca acacattgtt
     3661 ttatcagtag ggctaataac tgatcaaggt ttagcagaaa caggaggaaa tggagttatt
     3721 tttttatttg ccatacctga agaaatgacg gctgaagttg agaatgaatt atttgagcta
     3781 tacgatatcc tatttgaact cataaaaatg ggaatagatg agtttattga attagcaccg
     3841 acacaagaat catttttaaa actaatcaaa agtaaaggag agagacttca tgaatgatat
     3901 gtatctattc atagttttcg cagcttcagc ttatgtgctg caagcagtta tcagttggaa
     3961 gcaactaaag catttcaatc aaacatatag cgaactgcga agaaaaggaa aagtagcgat
     4021 tggtaaacga cctgggaaaa tccgttcagg cacgattgtt atgctagcta tagacgatca
     4081 tgatcaagtt ttagaagtca gaaaaatgca aggggttaca tctttggcaa aatttcattc
     4141 attacctgtc tatgcgggtg aagatatcca ttacatcgat cgttaccatc cctcagttcg
     4201 agcggaagat cgtttgacac aagcagcttt agaaaatgcc agagaagtat acttacgaat
     4261 agaggcagga aatgaaaaaa gtgaacaacc ttcattgcct tttattgcta tgctcaataa
     4321 ttttcggata tggaaaagta cgatcaaaga aaaaaggagt gacaaacgat ggatatcata
     4381 acaaaatttg ctgagggatt tatgaggctg ttccaaacag gtgcagaaac attcataagc
     4441 tggatgacag gaatcgtacc agttgtcttg atgctgatgg ttgcaatgaa tacgctgatt
     4501 gcttttttag gtgaagaccg agtaacgaaa gtagccaaat tatcagcgaa aaatccgctt
     4561 actcgttacc ttgtgttacc gttcttatct gcttttatgt tgggaaatcc tatgtcattt
     4621 acaatggctc gtttccttcc agaatactac aaaccaagct attatgctgc tcaagctcaa
     4681 ttttgtcata cgagcaatgg tgtattcccc catattaatc ctggagaatt gtttgtatgg
     4741 atggggattg ctcaagggat cgaaacctta gggctaaatt cgatggagct ggctatccga
     4801 tacttgcttg tgggactgct gatgaacttt attggtggtt ggataactga ttttactact
     4861 ggatttgtat gcagacagca aggaatcgtt cttagcaaaa aagtagagct ttcagttgat
     4921 tagagaaagg agagttttaa atgtatggtg atattaagat cgtaagagga aacggcgggt
     4981 atggaggtcc tttgattatt aggccgacaa aagataaaca caagttcatc tatgtcacag
     5041 gtggaggtga gaggccaatg atcgtagata aaatagaaga actgacaggt atggagtcta
     5101 tcaatgggtt caaaacatca atacctgata gtgaaatcgc gcttgcaatc gttgactgtg
     5161 gagggactct tagatgtgga atttatccta aaaaagggat accaaccatc aatatcgttt
     5221 ccacaggaaa aagtggccca ttagcacaat atatcactga agatatttat gtttcagcag
     5281 tagggttaga acaaatacag ccactaatgg aaactgatga taacaaagaa acagtaaata
     5341 ctgcatctcc agataaaaag tacaaatatg atacttctaa aaagataacc gagcaaaaag
     5401 cagagcagca aaactggata gcaaaaattg gaatgggcgc gggaaaagta gttgctacat
     5461 tcaatcaatc agcaagagaa gcagtagata cgatgatccg gacagtgatc ccttttatgg
     5521 ctttcgtttc ccttttgatt ggtgtgatac aaggttctgg gatcggtaat gtatttgcta
     5581 acattatggc acctttagca ggaaatgcag ttggattaac aatcattggc tttatttgtt
     5641 cactgccatt tttatctcct ttgcttggtc caggggcagt tattagccaa gtgattggaa
     5701 cattgatcgg tgtagagatt gggaaaggaa atattcctcc ccaattagca ttaccggcac
     5761 tatttgctat caatactcaa aatgcatgtg attttattcc agtcggtcta ggtttggaag
     5821 aagcaaaagc tgagacagta gaagtaggtg taccatcagt tctttattca agatttctta
     5881 acggcatccc acgagttatc attgcttggg tagcaagttt tggattatat agctg
//
LOCUS       DAYYAR010000143         5808 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000143 DAYYAR010000000
VERSION     DAYYAR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.145"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACHAT0_002503"
     CDS             complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACHAT0_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8452407.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2240..4426)
                     /locus_tag="ACHAT0_002504"
     CDS             complement(2240..4426)
                     /locus_tag="ACHAT0_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8452408.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACHAT0_002505"
     CDS             complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACHAT0_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8452409.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4648..5088)
                     /locus_tag="ACHAT0_002506"
     CDS             complement(4648..5088)
                     /locus_tag="ACHAT0_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8452410.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5162..5701)
                     /locus_tag="ACHAT0_002507"
     CDS             complement(5162..5701)
                     /locus_tag="ACHAT0_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8452411.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1503 a         1305 c         1015 g         1985 t
ORIGIN      
        1 tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt tttcattatt atctgatttt
       61 caatgtcaaa gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac
      121 tgctggactt aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag
      181 aatattgtaa cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc
      241 tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga
      301 atcgattgcg atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact
      361 tggcgcatca ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt
      421 ctggacaaca gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa
      481 ataatctgct actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat
      541 ttttctttct ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa
      601 agcaacgatt gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc
      661 ttggtaaggt ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc
      721 attaactatt ttcacttgtt gttttttgac tgttgcctct agcccgactc ctgaaagatt
      781 ttttagattt tcagcttgat aaggcttgat ttcatgttct ttcaaataat tcataatgcc
      841 aacagccaat ggatgattgg cattcgcttc caaggctcct agatattgaa ggatttcatt
      901 tttattgaac gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag
      961 tgttcctgtt ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg
     1021 gtttttcaaa agtaatccgt ttttagctgc tattgatgtg gaacgagcaa caactaatgg
     1081 gatagctaaa cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa
     1141 tgcattagga agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa
     1201 tgcgacataa aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc
     1261 tccttgtgct tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc
     1321 agttacttcg atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt
     1381 ggcttgtttt ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc
     1441 acctttcaag attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc
     1501 accttcgttt acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt
     1561 cactgattct ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc
     1621 attcatttca atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa
     1681 gaaatccatg acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata
     1741 aatatatgca actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt
     1801 caattccatc ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc
     1861 taagatcaat acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc
     1921 catcattggt gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg
     1981 tttgaaattc cccatgtgca tggaatgatc catgccgccg tgttcgtgat ccatgtgcat
     2041 cgaatggtcc atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga
     2101 atgatccatc tgactatggt catgtttcat ctccatctgt ttttcatgag attctgtact
     2161 ttctttgttt actacttctt ttttatgctc atgttttttc tccatatcgt tcccattcat
     2221 ttttactcca tcccttctct tactttttca tgtgtcgatt taagcacagg gagttcaaca
     2281 atacactgac ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta
     2341 gtgcggcaaa aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga
     2401 tttttctcaa tgtcaaccta gacaactcga ttgtttgtgc aatggatgcc aaactgctat
     2461 tcatcagcgt cacatcggct gtttccatcg caatgtccgt tccacttccc attgccatac
     2521 cgacatcagc taatgctaag gccggtgcat cattgattcc atcgcctgcc ataccgactt
     2581 ttttgccatc tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag
     2641 caaagatatg ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt
     2701 tgtctcctgt cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt
     2761 tgacttctag tttgatttga tcagccacgg caatcaaccc gatcacttgc ttttcatcag
     2821 ctaaaaacat gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat
     2881 caaatgatag gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt
     2941 taatcgtacc gctgattcca gcacctgggt gggctgtaaa atcaatcata tcgtatgttg
     3001 ctgcttcttg cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt
     3061 gttctaaagt ataaaaaata gataggactt ctttagaacc gactagatct gtaactttgg
     3121 gctttccttc tgtaatcgtt ccagttttat ccaaaacaat gctgtctaag tgagcagctg
     3181 cttctaatgc ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta
     3241 ccataatagc tgttggagtc gctaaaccta atgcacatgg acacgcaatg actaatacag
     3301 agacactatg gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg
     3361 tgataaacgc gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt
     3421 tttggattgg ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata
     3481 ctgtctgctt gccgacttgt gtaacttcca tgtgaagtag accattcgta ttgattgtcc
     3541 cgccaaagag cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac
     3601 tttcatctat cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag
     3661 gacggatgac tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc
     3721 cgtttcttat cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac
     3781 ctgttttcgt ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca
     3841 tactgctgct ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc
     3901 tcaatgcaaa ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg
     3961 tcttgattgc atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag
     4021 cagacagcac taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc
     4081 ctaacatcat agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa
     4141 gatcgaatag catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg
     4201 cttcatcgta caaaattgca ccatagccga tgttttctac gctttggatt aatttttcag
     4261 tggtcgtccc ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga
     4321 ccacaccagg ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca
     4381 tacctgtaat gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc
     4441 tttataaccc aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc
     4501 aaacttgacc gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag
     4561 tgcgcttact gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga
     4621 aaattgttgt ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg
     4681 gatacagtta cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc
     4741 attgatatct tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat
     4801 cctttttgca cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc
     4861 cgagacagct ggataataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt
     4921 ttttaccaaa cgacccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc
     4981 taagatctgc gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat
     5041 ctcccactct gaatcactga tctttactgg cgttttgact ttttccattt atttcacacc
     5101 tttcgtttac aaatgtaatc attatcttat gaatacagat tacacttgta aacaaaaact
     5161 gtcaattatt tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg
     5221 ttttaaggat gatcagttta tttcttgcat tagctacttg gatcagctgc tgtattttcg
     5281 gttcattgtt ttcttcaaaa tgccagataa aagataaaaa ttctaagtat tccctgttta
     5341 gtttctccga aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta
     5401 gagaacgcca aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct
     5461 tatctgcttc tttaatcctt tcctctaatg tagaagtata atttccttca acgatccatg
     5521 acggattaga taccataaac aattgctgtt cttgcaaaaa ccactttttt gcctgaatac
     5581 tgtagtctgt tgtatgccat aaaaggtcta gcggcagtag cggaaagtcg gtgatttctt
     5641 tgatcttcct tgcgagggtc gattttcccg acccactttg tccaatcacg atgatcttca
     5701 ttcttctccc tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa
     5761 aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaa
//
LOCUS       DAYYAR010000144         5800 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000144 DAYYAR010000000
VERSION     DAYYAR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.146"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            310..1302
                     /locus_tag="ACHAT0_002508"
     CDS             310..1302
                     /locus_tag="ACHAT0_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8452412.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1362..2783
                     /locus_tag="ACHAT0_002509"
     CDS             1362..2783
                     /locus_tag="ACHAT0_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8452413.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2776..4275
                     /locus_tag="ACHAT0_002510"
     CDS             2776..4275
                     /locus_tag="ACHAT0_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8452414.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4314..5708
                     /gene="uxaC"
                     /locus_tag="ACHAT0_002511"
     CDS             4314..5708
                     /gene="uxaC"
                     /locus_tag="ACHAT0_002511"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8452415.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1905 a         1071 c         1191 g         1633 t
ORIGIN      
        1 gagagactcc tttggtaggg tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa
      121 cacaaaatat tatagaaccc taattaaatg agcattcccc gctttcaaga atagcataat
      181 taaaaataaa aatccaagta atttttaacg tgtgcaatct tttcatatca aagatctatt
      241 gacttatcgt agttatcgta atatgatgaa tgcgttaaca aaaagtgaaa aagtaaggaa
      301 aatgatggaa tggtaacgat caaagatatc gctaaagcag ctggcgtttc tcatacgaca
      361 gtttctcgtg ctttgaataa cagcagttta ataaaaaatg aaacaaaaca aaaaatacga
      421 caactagcta aggaaatgaa ttattcgcca aactatagtg caaaaggttt agtgaatcaa
      481 aaaaaatatc taatcggact atttttctca agtttgcata aaggaacttc atcaaatttc
      541 ctagcagatt ccattgcagg catccattca gtattagaca caaattacag tttatctgtt
      601 gaaagtatcg atgttgttga tttaacagaa atcaacctgc aacgctacga tggactgatc
      661 gttatgagtc aaagtgatga tgatcagcct ttcatcgact atctcaaaga aaaagaattt
      721 cctttcgttg ttgtcaatcg tcacatcaat gatccagcgg ttatcaacgt cactgccgat
      781 gatgcggccg gtgttatgca ggcagtagat tacggtattg gatgcggaca tacgaagatc
      841 gcttatattg gcggaaagaa aaattttcgt tcgacaaaag aacgggaaaa aggggtattg
      901 aaaagtctaa aaaaagcgag catccctgtc aatctagcct attttttaac aggagattac
      961 agcttagaaa gcggcctcca aaacatggaa tacctattat cactttctga cctgcctact
     1021 ctggtttttt gcggcaacga cgacatagcg attggtgcat tgagagctgt acatttagct
     1081 ggcctccgtg ttccccagaa catttctctg atcgggttcg atgattctcc agtcgtctcc
     1141 tatctcaatc cgccgcttac caccgtacgt aagcctttga aagaaatgtg tcaagaagga
     1201 acaaagcttt tactagattt gatcgaaggc aggacgatcg aacaatctaa aattgagctc
     1261 tccaccttgt tgatccttcg tgagtcgatc aattacttgt aaactacaag taattttata
     1321 taaaaatgtt aacgtgtgca taacacagaa aggaagttta tatgaaaaaa ctgacaaaag
     1381 aattagtgaa aaaaaacgaa gcacctgtaa aagttcttca gtttggtgaa ggcaatttta
     1441 tgcgtggatt tattgattgg cagattcagc agctgaataa ccaacaattg tttaaaggta
     1501 atgttgcaat cattcaacct ttagaacaag gtcttggaaa tatgatgaac cagcaagata
     1561 atttatatac ggtcatttta caaggactca gcaatggcga aatcgttgat acttccgaga
     1621 ttatcaccgt agtagaacaa atgatcaatc cttatgatga atgggatgct tatctatctc
     1681 tagcagaaaa tgacgacttg gcctatatcg tctccaatac aacagaggca ggaatccaat
     1741 tcgtggagac tgataattta acagatactc ccccacagag cttcccgggc aaactgacag
     1801 cctttttata ccgacgtttc aaattaaaca aagctggctt taccatcatt ccttgcgaac
     1861 tgattgacag aaacggcgaa cgtctaaaag aaatcatttt acgctacgca gaaaaatggg
     1921 atttagaacc tgcttttgtt gagtggttag aaaaagaaaa tgttttctgt tgtagtttag
     1981 ttgatcgcat cgtcccaggt tatcctcgcg aagatgcagg tgttttcaat caacggcata
     2041 actatgaaga tcaattgatg gtcaaagcag aaccattcat gctttgggtg atcgaagggc
     2101 ctaaagaatt ggagaatcaa ctaccattca aacaggctgg cttgaatgtc atcatcacag
     2161 atgatatgac tccttatcgt gaacgtaagg tgcatttgct taatggaccg catacagcga
     2221 tggtccctct tgctttgttg gcggaattgg aaactgtaga agaagtaatg aaagatcctt
     2281 tattctccgc atatgttgat cagttattca atctagaatt gattcctatg ctttctttac
     2341 ctaaagatga attagccatt tatgctgacc aaatcaaaga acgtttcttg aatccttttg
     2401 cccatcacaa attagaagcg atttcattaa atagtgtttc aaagttcagc acccgtctgc
     2461 ttccagtgtt caagaagtat atcgaagaac aaaatcaggt accaccgtta atcactgttt
     2521 cactagctgc gctacttctt atgtatcgag gcgatcaagt aaagccacat gatgatgaaa
     2581 agacgataag cgaatttact gatgcttggt ctgataatgg aacagctatt cctcgattat
     2641 tgcaaaacgc tgcgctttgg ggagaagatt tgtctcaaat accgaatgtg acagatacag
     2701 ttcaagaaat agctgcaaag atcgatcaaa aaggtgtccg gtcagtatta caagaaatga
     2761 aaggaacgat ggtccatgct tgaaaccctg caaccaacaa tgaaattaaa tcgacgagac
     2821 agtgtggtcg tcgcattaac ccctattcca aaacaaacac aactgacgat tgatcagcaa
     2881 acgatcacca ctcttgaaga tatcccgcaa ggtcataaga ttgcattggg tgatttgaaa
     2941 gctggagaca acgtgatcaa atatggctat ccaatcggtc atgtgacgac agacgtaact
     3001 gccggtcaat ggctgcacac tcataatgta aaaaccaatc tttctggtga attggattat
     3061 cgatatgaaa aagatgtcca tccaagtcat tacctttttg agaaccgaac atttcaaggt
     3121 tacttgcgaa aaaatggaaa agtagggatt cgtaatgatt tgtttttagt acctaccgtt
     3181 gggtgtgtga atggcattgc tgaactgatc gtcaaacagt tcaaggaaaa acatccagat
     3241 ctgggacagt ttgaccatat tacgatttta aagcatcctt atggctgttc gcagcttgga
     3301 aaagatcatc aaaatactcg agaaatttta gcggacgcag taaatcatcc caatgccgga
     3361 ggagttttag tatttggatt aggttgtgag aacaatacag ttcctgaatt caaaaaaatt
     3421 ttaggtgaat atgatgaaga acgagtgaaa tttctagtgg ctcaagatgt ttacgatgag
     3481 atcgaacaag gtgtggctct attagaagag cttttaacag cggcggaaaa tgaccagcgc
     3541 atatcagtcc cgctctctaa gttgaatgtc ggtttgaaat gcggcggttc tgatggtcta
     3601 tcgggaataa ctgctaatcc tttacttggt gcattttctg actacttgat tgctcaagga
     3661 ggcagcacga tcttaacaga agtgccagaa atgtttggcg cagaacaagt attgatggca
     3721 cgagcggaaa atgaagaagt ttttgactca atcgttgact tgatcaatga cttcaaacag
     3781 tatttcttgt cttacgggga accagtttac gaaaacccat ctcctggtaa taaagcaggt
     3841 gggatcacta cattagaaga taagtcttta ggctgcacgc aaaaatcagg aagctctcct
     3901 gtcgttgatg tgctacaata tggcgaaaaa atcagaaaac cagggcttag tttattgcaa
     3961 gctcccggga atgatcttgt agctgcctct gctcttgctt catccgattg ccagttggtc
     4021 ttgtttacca caggaagagg gactccgttt ggctcatatg tcccaacggt caaggtatct
     4081 acgaatacaa ctctttttga acgcaaaggt cattggatgg attttaatgc aggcgtctta
     4141 ttagaacaac caatggaagt aatcttagaa tcgtttgttc aaaaaatcat cgctgtagct
     4201 agtggagaag aaacgaaaaa tgaacaaaat gacgttcgag aaattgccat ttttaaaaac
     4261 ggagtaacac tttgattttc ataaaacagt ataaaaagaa agaaggaaga tttatgtttt
     4321 tatctgatga ttttttatta aaagacgatt gggcgaaaaa gctttaccat tcctacgcaa
     4381 aaaacatgcc cattattgat tatcactgtc acttagatcc aaaagaaatt tacgaaaaca
     4441 acaatttcag caatctgaca gaagcttggt taggaggcga tcattacaaa tggcgcttga
     4501 tgcgagcttg cggcgtgcct gaagaaaaaa tcacaggaaa tgcttctgat tttgaaaaat
     4561 ttttagcttg gtgtcaaacc gtaccaaaaa ttatcggaaa tccgctttac agttggtcac
     4621 atcttgaatt gaagcgtttc ttcggtattg acttgttgat aaatgaagaa aatgcagaga
     4681 aaatttggac acaagcaaat gaaattttgg cagctcctga ttttcgccgt cgggaaatcg
     4741 caaaaaaatc gaatgtagaa gtgatttgta caacagatga tcccgcagac gacttgcact
     4801 atcatcagtt gcttgctcat gaagaaccag atttgaaggt tctaccttca ttccgtccag
     4861 ataaagcact caatattgaa aaagaaggat ttaatgcatg ggtcacacta ctggaagaag
     4921 cagctgacca aacgatcacc acctatcaag aactgataga agtcttaggg cagcggatag
     4981 aatacttcca tcaacatgga tgtcgtgttt ccgaccacgc tttagacatc ttacgctata
     5041 aagaagcgga tgaaacagta ttagaagaga tttttcaaaa agcacgagct aacaaactat
     5101 tatcttcaga tgaaatcgat gcttatctta cagaaacact gatacatttg acccacttct
     5161 atcattctca taattggacg atgcagctgc acatccatgc ttatcgtaac tgtaatacac
     5221 aaatgttgga aaaattaggg cccgataccg gctatgatgg aatgaatgat ctttctttga
     5281 ctattcctct gcaaaaatta ttgaaccgtg cagaagaaac ggatcagttg ccaaagacga
     5341 tcctttattc attgaatccg aatgactatc ctgcgatttt agctttgatg ggctgtttcc
     5401 aaaaagaaca gaatggaaaa ctgcagttag gttctggatg gtggtacaac gatactcgag
     5461 ctggtatgcg cgatcaattg acacaatttg ctgatggtag tgctttgggg aattttgtcg
     5521 gcatgctgac agattcaaga agtttcctgt cctacactag acatgaatat ttccgtcgtg
     5581 ttctttgtga atttttaggc gaaatggtca tgcgaggaga agcacctgaa gatgaacaac
     5641 ttttaggaag tctagttcaa gcgattagtt atacaaatgc taaaaattac tttggctttt
     5701 ttgcctaatt gattaatagt aacagtaacg aatcaaaaac aggatagtga aataacggaa
     5761 tccgtttatc cacgttttag agaagaagac ggaccaaatt
//
LOCUS       DAYYAR010000145         5700 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000145 DAYYAR010000000
VERSION     DAYYAR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.147"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1329
                     /locus_tag="ACHAT0_002512"
     CDS             28..1329
                     /locus_tag="ACHAT0_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8452416.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(1398..1997)
                     /locus_tag="ACHAT0_002513"
     CDS             complement(1398..1997)
                     /locus_tag="ACHAT0_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8452417.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            2252..3133
                     /locus_tag="ACHAT0_002514"
     CDS             2252..3133
                     /locus_tag="ACHAT0_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8452418.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            3146..4756
                     /locus_tag="ACHAT0_002515"
     CDS             3146..4756
                     /locus_tag="ACHAT0_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8452419.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(4833..5492)
                     /locus_tag="ACHAT0_002516"
     CDS             complement(4833..5492)
                     /locus_tag="ACHAT0_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8452420.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
BASE COUNT         1693 a         1090 c         1067 g         1850 t
ORIGIN      
        1 aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc aaaaaattat tttaggagcc
       61 acttctgctt taactatcgt tattttaatt ctaagtttgt tttttgtcat ccgatcatct
      121 acaaaaaaag aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa agaaccgaca
      181 gatgataagc aacaactagt tactcaagca aaaaatctgg ctcaaagcta cgattatgac
      241 caagcgattg cgttattgga aaaagatagt aagaacggat ctactgaaac acaacagctt
      301 cttcaaacac taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc tactcagatc
      361 tctcatgtct ttttccacag cttgatcgtg gacccgcaaa aagccttcca ctctcaacag
      421 gcacaaggat acaaagatta tatggtgact gttgaagaat tcaaccgttc gatcgaacaa
      481 ctttatcaaa atgattttgt tttagttaat ttgaatgatt tgatccaaaa agacgaacaa
      541 ggaaatcttt cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt ggttctttca
      601 caagatgatg tcagctatta tgaatatatg gataattccg gttttcctag ccgattggtc
      661 gtatcaaata acaatgaaat caaaaatgtc tataaagaga aagataaaga agaaatcggt
      721 gactttgaca tggttcctct gatcgatgcc tttgtcaaga aacatcctga tttttcttat
      781 cgaggcgcaa aaggtacctt ggccctgaca ggttacaacg gagttttagg ttatcgaaca
      841 tcaaaatctc aatatggaga caatgaaaaa acaaaaaaag agattaaaga agctaaaaaa
      901 gtggcggaac agttgaagaa agacggctgg agctttgctt cccatacatg gggtcacttg
      961 aatatgactc aagcttcact agaagatgta aaaaaagaca atgaattgtg gcagcaagaa
     1021 gttgcaccta tcctaggaaa aacaaatatt ctgatttatc catttggtgc agacatcagc
     1081 gactggcagc cttattcagc agaaaatcaa aaatttgctt acttgaagag tcaaggtttc
     1141 gatattttct gtaatgtcga tgcttctaca ccagcttggg gacaattagg tacggatttc
     1201 tatcgaaatg ctcggatcaa tatcgatggc attcgttttc aatctgattt agacggaaaa
     1261 aatcctattc ttgatccatt catcaatgtc catgatgtct atgacaaaga agcacgcgaa
     1321 tctgcttgat tttacgtcat ttatgaacca aaaaagccga actgacattt tatcgtcagt
     1381 ttcggctttt ttatgcttta tttcttcttc ggtgcgattc ccttgatcag gacttctgtc
     1441 cattctgctg tatatggaag cagttttgcg tatatctccg gcttagaaat tccttctacc
     1501 attgcttgaa aatagaggat caagaactca tccgaatatt tcaaatccac ttttccttct
     1561 ttccgccctc gatggaaaag atcaagcatg atactgaaac tttcttgcgt atacgcatcc
     1621 atcgcgtcag aaaattcttt tttttctttt tttgtatagt atgtcatcaa atcgatataa
     1681 aattgtttgt tcactcgatt gagataacgg attttgtttt cactcatggc aatcagtgtt
     1741 tcttcaaatg gaagattttc cgcaatcaac gctttcgctt cttgccctaa atcattcata
     1801 aatgcaaaaa atacttcata aatcagccct tccttgctgc caaaatattt aaaaatcgtc
     1861 gctttactta cctgagcgtt ctcagctacc ttatccattg tcactccagc ggttcctatg
     1921 tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt tctttttctc ttgtgctctc
     1981 ttttcaaaac cgttcatttg tagagcctcc cgtaatttta aacttttata atatatatag
     2041 ttcaaattta ttctgaggtt tattataact atctattgag aaagtatcaa gctagcaatt
     2101 ccttgtttat gaattcattg acagaaattt ataaaaagaa tattatgtac taaaataata
     2161 aaaaaaagtt ttataaaaag gtataaaacc tttttattaa actatttaat cttgtttagt
     2221 atatattttt agaaaaagga gtggggtttt tatggcagaa atcgtcaaag tcacacattt
     2281 gaaaaagagc tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg tacattcagg
     2341 tgaagtcgtt ggatttatcg gaccaaatgg ttctggaaaa tccacgacga ttcggacact
     2401 tttagggatt ataaaaaaag acggcggaac ggcaacgatt tttgggcaag atgtatggga
     2461 agatagttta tctatccatg aacgtctttc ttatgttcca ggcgatatct ctttgtgggg
     2521 aaatttgact ggaggagcga tcatcgatct cttcatgaag cttcatggaa aagggagtgc
     2581 tcaaaaaaaa gatgaactga ttcaacgttt ctctcttgat cctaagaaaa aagcaaaaaa
     2641 ctattcaaaa ggaaatcgcc aaaaagtggg cttgattgct gctttggctg ttgattcgga
     2701 tctctattta tttgatgagc ctacttctgg tttagatcct ttgatggaaa cgattttcca
     2761 agaagaagtt gaaaaattga aagaacaggg caaatccatc ctactttctt ctcatattct
     2821 cagtgaagtc gagcgactag cggataaagt cgtgatcata cgcgaaggaa cactcgatga
     2881 agaaggaaca ctcgatgaat tgcgtcattt gactcgttcg accatcacta tagaaacaga
     2941 agcagagaat agtcacttag cacaattgaa cggtgtccat gactttgtgc aaaaaggtca
     3001 cctcgctcaa tttgctgctg ataacgaagc tttcgatgtg attttagctg aagcgtccaa
     3061 attaggtgtg aaaaaatttg aatctattcc accgactctt gaggatttat tcatgagaca
     3121 ttatgaagga tgaggaggaa gcgaaatgac tgataaattt tcacgctggt ccaccctttt
     3181 ttctcaaaac cttagccgtg actggaaaaa gatcctgata tggctagtcg gtgtcactgc
     3241 tttttctggt ggttttgtcc ctgcgtttga agaattgact tccggacaag gattagctgg
     3301 attatacgaa accttgcaaa acccggcaat gattgcaatg gtcggaccaa ctccagcttc
     3361 gaatgctgct gagtatactt taggagcgat gtatgcaaat gaaatgttgc ttttttgcag
     3421 tttgtttgct gctgtgatct ctgttttaca cgtggtgagc caaacgagaa aaaaagaaga
     3481 acttggtctg attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa actctttagc
     3541 tgtcatcgct gaaagtattc tgatcaattt gcttttagca atatcgatca gctgtacgat
     3601 gatttttttc caagcggatt cgatcactgc agaaggtgct tggctttttg ggctatctgt
     3661 aggtagtatg gggattttgg ggagtgccgt tgcactgttt gctgcacaac tgatgccaag
     3721 ttcagccagt tcgaatggtc tgtctttagc gtttttaggt ggactttacc ttctacgagc
     3781 aataacagat gtagctgatt cctccctttc gatgtggaat cctttaggct ggtcttattt
     3841 gacctatcca tttacaacta acaactggat tcctttgctt tatttgtttt tgttcagtct
     3901 aatcttgata atcagttcat ttgttttaga ggaacatcga gatttgaaca ccggctatat
     3961 ccccgaacgt gaaggaaaac cgtatgctaa acgttctctt ttatccttgc gtggtttatt
     4021 attctatcta aataaaggga tgacaatcag ttggcttatc acttttggtt tgatgggggc
     4081 ggcatacggc tctatctacg ggaatatgca acagttttta gaaagtaacg acttaatgaa
     4141 acagatgttt atccaatctg gcgtctcaat tgaaaaaaac tttacaagca cgatcatggt
     4201 agtcttgatt tgtctagctg ctattttacc aattgctagc atcaatcgtt tatttacaga
     4261 agaaacccac ggacgtatga atcagctgtt cgcaacaaaa atttctagga accaacttta
     4321 ttggaccaat atgttactag ctattctatt tgctatattg gcaatcctga ttagttctgg
     4381 tgcactaggg ctttctgctt tggcttcgat gaaggatgcg aaagacatgg atctattgat
     4441 gtttcttaaa gccgggttca atttcctgcc tgccgtttta gtgtttatcg gaattagtgg
     4501 ctttctattc ggatggcttc ctcgaatcgg taaacttgct tatatctact tagcttactc
     4561 ctttgcactc aattactttg gcggaatttt ggatttacca gaatggtttt ccaaaacagc
     4621 tgtccctagc tgggttccac ggatgccaaa cgattctttt gatctttccg tgttccttac
     4681 gttatcatgt atcagccttg cactgttcgt ccttggttct attggttata ggcgaagaga
     4741 cttactagaa ggataataaa aaagcatgta caatttcatg ctagcctcca aatctgggtc
     4801 aaaaatccaa catttggagg tcattttttt atttacaagt cttctaactg ctctttatca
     4861 atgatccaga cttctcgatt ctttcttttt acataaccat tctcctctaa taatttaaat
     4921 tttctagata aagtttcggg ggtcgtaccg ataaatgcag caagttcttt cattttcatt
     4981 ggcaggaaaa agtgatcctt ccctgtcgct ttagctaagt caagcagata gacagcgact
     5041 cgttcttcta ctttttccat catcaaaaaa gttgtctgct gctgtacttt tgttaatttc
     5101 tcaacgttga ttgctaaaag tttcagacta agggcgggat aatgcagtaa gagttccgag
     5161 aaatcttttt gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc aaataaagac
     5221 tcatttggaa cgcctagcaa ctggctctct ccctcatatc cgcccggctc tattactcgt
     5281 aaaagctgtt cctttccgct agcagataat tgatacactt tcatactgcc ggaggccacg
     5341 atcactaatt gaggttctcc attcggcgca agaatctgtt ctcctttttc cacatagtga
     5401 tgtttgacaa gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt gaacaaagga
     5461 acaaatgaaa cacataaatg atcatgactc ataatctctc ctccttctaa acagctattt
     5521 ctaataaaga aacataactt cccttattcc aactgataat tttcttttat catagcataa
     5581 acgtcaagcg aaccagatac cagcaaatag agtggatttc tttttgtccc ttgctttatt
     5641 ttccttcttt tgagacctta agtaattgat tcctatcaat tttttaaata tttacttttt
//
LOCUS       DAYYAR010000146         5672 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000146 DAYYAR010000000
VERSION     DAYYAR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.148"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..1491)
                     /gene="tyrP"
                     /locus_tag="ACHAT0_002517"
     CDS             complement(70..1491)
                     /gene="tyrP"
                     /locus_tag="ACHAT0_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8452421.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="ACHAT0_002518"
     CDS             complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="ACHAT0_002518"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8452422.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3902..5158)
                     /gene="tyrS"
                     /locus_tag="ACHAT0_002519"
     CDS             complement(3902..5158)
                     /gene="tyrS"
                     /locus_tag="ACHAT0_002519"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8452423.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5270..5475)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1618 a         1186 c         1001 g         1867 t
ORIGIN      
        1 aaaaactatt cacttggcag gacctcctac taaatcagcg acaaaacgtc ggctgaaaga
       61 cttatttttt tatttttctt ctaaactttt ttctacctca atcccccaag aatctttctt
      121 gaagtgattg atgatcaatg gaataacgaa catgaccact gtgatagctg ctactaataa
      181 tgcatcactc aatgtcgctg tacctaatgt agcaaatacg acaacgatga tagagaagat
      241 cagcatgcag ctgactaccc aagctaatcc gtttccgttt ttaccaattc ggtaaggacg
      301 ttcgaattct ggatgtttct tacgcatttt aagcaatgaa acagcaatca aaatgtacac
      361 gatacaataa ataacagttg tagcatttgt aagtgtcaag aatacagcac ttacgtcatc
      421 cattacacca taaagcaaag caaataatga aataacgatt gattgtgtta gaacgacatt
      481 tctagatacg ccgtatttgt tttctttatg gaatccgaat tttggtggaa ggaatccttc
      541 acgagctact tggatgattg tttttgatgg tccagtaacc caagctgata attgtaaaag
      601 cacaccaata aataccatga agctgaagat atttccgatc acttctggta agcctaggat
      661 ttgacagtca atcaagatag gctgcgtgat atttgctaat tccatttttc catctggtac
      721 gacatttgct acaagcatcg cattgatgac attcaataaa actaatccga tcaaagcgat
      781 aaatacgcct tttgtataat tttttgttgc gtctttcaaa cgaggcatgt aaacagaact
      841 catttcgatc ccaacaaaaa taaatgtgat accagctaag tatttcaatg aatcaatatg
      901 ttccaagtca gggaaagctt ttgaccatga aaagtcacct agatagccgt ttgagactag
      961 tccgactttg aaggctgcca aaacacctaa aacgaacatg atgactactg ggatatatac
     1021 ccctagccat acaccgatgt tcccgccgac ttttgccata tcgaatttca agttcaagat
     1081 cgtgatgatc cagtagataa ctaaaataca acctaaaacg aaccagtgat tattgccaag
     1141 ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga ccattaccat
     1201 acctgggaac atttgcaccc ataatagcca tgcaacgaca aatccccatt tgctgcccag
     1261 tgctgttttt acccataact gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc
     1321 ggaaaccatt gatatcggac cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa
     1381 tgtccaaccg actgcggcta acgtaggaat actccgaacc gttgcacaca gtgccattgt
     1441 cagaccgata aaggtcccaa gacccatttt acttacttca gatttactca ccgttgacac
     1501 tctccttgat tttttggata aaataacggt gtctttgaag tagtttcagc aataaacgaa
     1561 acaattcagc gaatatttga ggggattctt tgagtttatt gcttgaaggg tttcttcaaa
     1621 gacaacctca aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt cagatccaaa
     1681 catgcgtcag aaacagttag tattcaagct gttttctaac atgattacct tgcttatttt
     1741 gcttcgcttg ccaataattg gtctgcgtag attttttcca atttttcttg aagagctgct
     1801 ttgatttttt cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg
     1861 ctggcacgca atacagttac ttttccggca cggttccatt cttcatctga gaatcctagt
     1921 tggttcacaa attgtaatgg gctgttgcca tagtctggga tagcaaagtc agtatgtgaa
     1981 gttaagaact cattaccgta aatgcttcct ttcacgtatg aagaataatc atatacgtca
     2041 tggttcaatt tattcattgc aactaagtca tcgttgcctt tttctttgaa tacatagtct
     2101 accatattga aatctggata agttaatgga tgaacttcga tttctttgtc tcctacttta
     2161 aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac cttcgattga tgcacccatt
     2221 aatttaccat atcctgtgac attcaaaggt aagacatggt gtgctgccca aacgctggct
     2281 gctgttgccc ctgcttttga accttccaag atataagccc caagtaatgc tgggatatct
     2341 gcgccttttt caaatacata tgtcgcaaag taagagataa cgtctctcat gcggatatct
     2401 ttgatgacga taccaccggc agaatatgga acatagccca ttttatgtgg gtcaatcgtt
     2461 acagattctg cttcttcgat tgctttatat gcactgtgta cttcttctaa gatataatct
     2521 ttgttttctg taaagacatt gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg
     2581 aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc cgccataagc agcatctacg
     2641 tgtaagtaga agtaaatacc gtctttttct aatacgcgac gcaatgcaac gattttatcg
     2701 ataccgtcga ttgctccttc ttctgttgat cctacaacac cgactacacc taggattggt
     2761 gttttttcag ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc catgcgatag
     2821 ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga taccgatgat gtcggcagct
     2881 tttagccatg aatagtgttt agtttgtgga accaaccatt ttcctaattt ttctaaatgt
     2941 tttccgctac gtgctgagtg agctttgatt tcgtcgattt tttctgggac gctgtctaac
     3001 agattcatga tttcttctgt tgacaagttc attaattccc aatcgctttt tccagcaact
     3061 agttcaggtg tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa
     3121 agtccttcta agtttgctaa agaaccatca gcaacgatgt ggccccagcc atctttatag
     3181 ctcattaatt ttgcaaattc cattcctact tcttcttcca tttggcttgt tgcaggagaa
     3241 gattcatagg caacgttatt accattccat agcatagcaa aattgtacgc tagtaaagat
     3301 ggcatcaatg tttcagagtt catatgaccc cagtaacgtc cggcattatg ccaaggaacg
     3361 gaatgggtac gcatccgcgc tgaaatttct gatagaacat cctttgtttt attcacagtg
     3421 tgttcaaaac tagcactgct tttttcttct ggcgtaataa taggcatgtc ttgaggcatg
     3481 tagttttgac gccagcctaa atgttcatcc accaattcat tcaacaaagc tttatagatt
     3541 tggccgttct cagctttgtc cccaatgaat agggcattta ggtttagatc tttcgacaat
     3601 gattcactca tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta
     3661 cacctgttct tctattctgt aaactaagtt ttggcctgat ttttaatcat attttaatat
     3721 gtatcaatta tccgaattgt tcacactttt gaaataatta aaaaaaacga gggaatttac
     3781 aaatgtaaac tccctcgcaa agattgtatt aacgacaacc agcgttctat tttaggatgg
     3841 gtttcaaaat tggaatgttg taatagtatt tccctttttc aaggtaaaaa cttactaaat
     3901 tctaatcaat tactttggcc aaaaagtaat ttttcttacc ttttcgaata accacaaact
     3961 taccatcgaa ttcatcggat ggatttatga caaaatttaa atcggtaacg cggtcaccgt
     4021 taatacttat agctccgttt gaaacatctt cacgtgcttg acgtttagat ggttcaattt
     4081 ttgtggaaac aagcagttcc acgatatttt caggtgtgct tgaaatttca acgttcggca
     4141 ttttaccgaa tccttgcgcg atttcttccg catttagttc tttaatattt ccagagaaca
     4201 atgcttgcgt tattttttgc gcttctttca agtcttcttc actatgcaca aatcttgtaa
     4261 cttcttctgc caaacggcgt tgtgcttcac gtttctcagg ttctgtctct acttttttcg
     4321 ctaagtcttc gatttcttct tgagacaaga aagtaaagaa tttcaagtat ttcactacat
     4381 cgcgatcatc ttgattcaac cagaattggt agaactcaaa tggtgaagtt ttcttcggat
     4441 caagccaaac ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca
     4501 atgggatcgt taaaccgaac gcttctgctt ctggtccttc taattttcgg attaggtcta
     4561 atccagctgt gatatttccc cattggtctg ctccaccgat ttgcaattgc acatcgtatg
     4621 ttttatgcag atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg
     4681 agattcctac ttccaaacgg ctggcaacga tatcttttgc taacatcgtg ttgatgttga
     4741 agtttttacc gtagtctctc aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt
     4801 tgttcacaaa tgtgatattt gcatctttac caaacaattt tctcatttga ttggaaagtg
     4861 cgtccacgtt atgttgcact tgttccatcg tttgcaacac acgttctgtt ttgcggccgc
     4921 ttgggtcgcc gatcgttcct gttccgccgc caatcagaat atatggatga tggcctgcca
     4981 attggaatct tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag
     5041 ttggatcaac gccgcaataa agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt
     5101 cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa ttcatcaata atattcattt
     5161 tactgacctc cgtgtagttt ttttatgaaa gcaggaacct gctagcatgt gtaattaatt
     5221 atttcttcag gataggatag gaaataaaaa aagtcccttg gcatataagc caagggacga
     5281 aatctcgcgg taccacccaa tttgtatgtc tagatatcca acatacctct cgaacattta
     5341 acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg
     5401 tactggtttg cagcgaccac cagctttctg aaacattggc tttaacaact acttcgtttc
     5461 gatcttcgtc ttgttattat taatatgtgt ctatcataga gcgcaacttt ttctttgtca
     5521 acagaaaaag gatatttttt ttaaattcct cattttttct tttttgaatc cgctctaatt
     5581 ccgaacattt tattttcaat tattgttaaa agaaataact ttttctgtat tttttctgca
     5641 atttatcgag aagtcacttt actttgcctc tt
//
LOCUS       DAYYAR010000147         5667 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000147 DAYYAR010000000
VERSION     DAYYAR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.149"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..765)
                     /locus_tag="ACHAT0_002520"
     CDS             complement(13..765)
                     /locus_tag="ACHAT0_002520"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8452424.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1129..3795)
                     /locus_tag="ACHAT0_002521"
     CDS             complement(1129..3795)
                     /locus_tag="ACHAT0_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8452425.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4136..5587
                     /gene="cls"
                     /locus_tag="ACHAT0_002522"
     CDS             4136..5587
                     /gene="cls"
                     /locus_tag="ACHAT0_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8452426.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1641 a         1192 c          991 g         1843 t
ORIGIN      
        1 aaaaagaacc cttcacgagt cgtgaaaggt tcttttttta tctgcggacc aaaggagcag
       61 tctataggtc agcttagtcg tactaaggat ttctcgtttt tcactgaata gtttgcgaat
      121 gtacctggtc acaacaggat cttttgcgta cgcgtcatag tatagcttca tcaattcttc
      181 agtaattgat tcctgtgtaa aataagtaaa atcttttttc ttcactttga atccttcatc
      241 aattaagtat gtttcaaatt gatccattac aactggcgat gtattaggat cttcttctat
      301 cattcctagt tgcttttcga taggataaaa aacatcatcg attgattcca ctactctgat
      361 tatcacgcac catttgcttg actttttcat caatttttct aattgaataa gatgtaccaa
      421 tgctggattt gctgctgtaa aaaccatttc atatgtctca tttgattgga taaattcttc
      481 taacgactgt ttgataaagg aaacatttgg caactcattt ttttctgctt ctttttttgc
      541 attttctagc attcctgaag aaaaatcgat caaatcatag cgattaattt cttttgctaa
      601 ggaaagaaat ctgccagatc ctccagcaac gtctgctgct gtttcagcag gcaggacttt
      661 ctccgttttc agataatttt ttacatcttc agcaatagtt acttgcgatt cttgctggat
      721 ttcataatat tcttcagcaa attcatccca ttctttttca ttcatttgat ttcctctaat
      781 tttttgttat atgttttcac ttttcggaat aaatcattct cctcttttat ctattctatc
      841 tcttttgatg aataataaaa tgattggctt tccgtctatc ataaataaaa aaagggactg
      901 tgaccaaagt cgtgtcaaag tccctctttc cgaataaaca gtgtccaatc agatgaccct
      961 cagtattagc tcaaaatgct gattttacaa cttcttcctt aaacggtgtt ctcctgccaa
     1021 ctcttcggtc acaagtcaca atattcaaca aacatgagaa gctgtttgtt gaaattgcac
     1081 cttgtgactc agaattaaac ggcaggctca caacctcgtt gattcgtttt atttatttcc
     1141 ttgtactcgt acaatgatca atttagtgat ttccatcaaa acaattgtca caagtgcgat
     1201 tcccatacat gtaaagactt ctgtcaatcc aaatgctgat gggatagaga agacttcttt
     1261 cagtcctggg atcattgtta agcagtaaag cgcgctacat acaactacag ctagtaagac
     1321 acttctgtta gagaagaacc ctgcttggat agctgtttgt gtgtttgaac gtgctgggaa
     1381 tgtttgcaat gtccgacacc aaatcaatgt tgaaaatgac atggctacac cgaattcagg
     1441 tgaatgctgc atgccaagcc attgagcaaa gataacagca atcccaatca gcacaccacg
     1501 atacgttaca gaaatcaatg ttttgtccgt aaagattcct gaatttggat ctcttggcgg
     1561 gcggttcatc gtagcaggtt cagctttttc tgtacctaat gcaattgctg gcagtgaatc
     1621 attgactaaa ttgatgaaca acagttgtaa tgctgtaaat ggattggacc agtcagcaac
     1681 taaggcaaag atgatcgcaa tgatcgcacc taaatttcct gcaaatagat aagaaatagc
     1741 tttcttgata ttatcaaaca cattacgtcc tacttctact gcgctgacaa tagaagcaaa
     1801 gttatcatcc gtcaatacca tcgctgctgc atcttttgcc acatctgtac ccgttcccat
     1861 agcaattccg atgtctgctt gtttcaatgc tggtgcgtca tttacaccat cacctgtcat
     1921 agctgagata tgacctttat tttgccaagc acgaacgata cgaattttat tctcaggtga
     1981 aacacgtgca taaacagtga ctttttctaa aacttcgttt agttgatctt ctgtcaattc
     2041 atctagttcc gtcccggtca aagccaggtt tccttcttca aaaatcccga tttgttttgc
     2101 gatagcaaca gctgttgttt tgtggtcacc agtaatcatg attgttttga ttcctgcact
     2161 tcttgcttct gttaccgcct gagtcacttc agcacgtgga ggatcgatca tagcaagcaa
     2221 accaacaagg atcaaatcgt cttcatcttc aaatgataaa tcaaaattat caagtggacg
     2281 gtaagcaaaa gctagtacac gtaatgcgcg tgttgaaaaa gcttcatttt gctcttggaa
     2341 ttttttcatc aattcatccg tcatcggaac gacttcacca tttagcaata cttttttact
     2401 gcgttggaag acgatatctg gtccgccttt aacaaacaag taatttttgt tatcaattgt
     2461 gtgtccagta gacattaatt ttcgatctga gtcaaaagga agttctgctt gtcttgggta
     2521 gttgtttcgg atttcctgat aaggctggtt taatttttca ctaaagtcga taaacgctac
     2581 ttccgtaggg tcgcccaatt tagtaccgtc ttcactgatc gatgcatcat tcgctaaaac
     2641 actgatttcg atcaagcgac gttcgtcagc tgtccatttt gaagggtcat ttgtgaaatc
     2701 cccagtatcg ccattaccta aaaagtagtc aacaactgtc attttgtttt gtgtcaatgt
     2761 accagtctta tctgtacaga tgatacttgt cgaaccaagt gtttcaactg ctggaagctt
     2821 ccgaataatg gcatgacgtt ttgccatttt attcgttccc aaagataaca cgattgttac
     2881 gatagactgt aatgcttctg gaatagcagc tacggctacg gctacggcaa acatcactgc
     2941 gctgacgata tctgcagtca tatctcctgt accttctcca agataaatac gaaacagttg
     3001 gatacctaaa atcaataaag acaatgccaa aatagccaaa cttagttttt tactgaattt
     3061 atccagacct cgttgtaacg gagtgacttg ctcagttgtt gattccaaaa ggccagcaac
     3121 ttgtccgatc tccgtattat tcccagttga agtcactaag aatttaccgc gtccgtaagt
     3181 tacgatcgtt ccactgaata ccatatttgt acgatctccg attggtaccg tacttgtgat
     3241 atccgttact tctttttctg cagaagtcga ctctcctgtc agcatacctt catctacttt
     3301 taatgatccc gcatctagta aacgtccatc agcaggtaca taatcgcctg cgtctaacaa
     3361 cacgatatct cctaccacaa gttcgtttgc cggaatcgtt ttttccttac catcacgaat
     3421 cactcgagca tcgggcgctg aaaggttttt caacgcttct aaagaacctt ctgctttttt
     3481 tgtttggaca acactgatta tagaattcaa catcaatact gcaaaaataa ctaagaattc
     3541 gatgtagtca cccatgacca tctgaatcag tgcgacaatc agcaatacga taaccattgc
     3601 atctttgaaa gtttctaaga acaatttcca agttggcttc ttcggctttt cttttagctg
     3661 gttcaagcca tctctttcta atttttcctt cgcttgcgtg gtgctcaatc catcggttga
     3721 gctctgcgtt tgctgcatca acgagtcgat cgtttcttta tagaccgctt gttgtgcttg
     3781 ttccttgttt tccaaatttc atcccccatt ttttgatctt ggccctagtc ctgtctgaaa
     3841 aaaaagagac ctaagacaaa tacgcatttg tcttaggtct caccatttca tacaatacca
     3901 gaaaaccatt tggttaactt actgttgata ttatcgcaac aaacgttgcc ggttactccc
     3961 ctaaggagat attcaactgt actaatatcc taccttatta acctacaaaa agcaactact
     4021 ttagcccgat ttacctacat tttcatgaaa ggagtgaaaa gtatattcac cttggtaggc
     4081 ttctttcttc tatcatgcta tactaatgtt attgtatgta aggaggaaca tttttgtggt
     4141 atctagtatt ataaccgccc tttatctatt aaatgcactt attgctttgg tggctatttt
     4201 gatcaaaccc cgagatgtag cagccatttg ggcatggctc ttagtattgt ttgcccttcc
     4261 tggggtgggc tttgtattat atttattttt cggacgtgga ttaacggata agaaaaaatt
     4321 ctatctccga caaagtgact tgaaagaatt agaaaacttt cagtctttta gaggagatac
     4381 cattgaacat tacgatcctg acatgggcga taaagacaaa cagcaatttg ttgacttctt
     4441 ctcttcatta aatcgtatgc cgctgacaag aatgaattct gtcactcttc tcacagacgg
     4501 acaagagaaa ttggattcac tgcttcaaga tctaaaaaaa gccaaacatt cgatccatat
     4561 cgaatattac gcatttgtga cagataatat cggccagcaa gtcttacatg ttttagaaga
     4621 aaaagccgca gaaggcgtgg aagttcgaat attatatgat gcatttggct ctcatggcac
     4681 aaaagcaaaa gatttcaatc gtctaatcaa aaatggtgga catgtccata catttgttac
     4741 ctcacaaagg gcattacttc gtttccgatt gaattaccat gatcaccgaa aaatcgttgt
     4801 gatcgatgga aagattagtt ataccggtgg tttcaatatt gccaatcaat atgtaaatac
     4861 aacaaaaaaa ttcggctatt ggcgcgatac gcatatacgg attttcggtg ccgcttcttc
     4921 tttgctccag cttcgcttct taacagactg gaacgtctcg gtacctgaag aaaaaaaggt
     4981 cggctatcat ttgaattatt tctttaaaaa agcagatcga gatgaatcta agcttgctga
     5041 tacatccatc cagcttgttt caagcggacc gaataacgaa agggaacaaa tcaagctttc
     5101 atttatcaaa ttgattactt ctgctaaaaa acgtgtttgg atacagacac cttaccttgt
     5161 tcctgatgaa agtgtcattg ctgctttaaa aatcgcaact gcctctggtg tagatgtgaa
     5221 aatcatgatt cccaacaaac cggatcatcc ttttatttat cgagcaacac aatattatgc
     5281 tcggcagctg atcaaggaaa atgtacaaat ccttgtctat gagaacggct tcctccatgc
     5341 aaaaacattg ataatggatg atgaaatctg catggtaggt tcagcaaatc aagatattcg
     5401 tagctaccga ttgaattttg aaacaagtgc tgtcatttac gatcctgagt ttttagaaga
     5461 acttgctact cagttcaaag aagatgagac acattgttca tccatgacaa ctgaaacagt
     5521 caaggaaatg tctaactggc tattattcaa gcaacaaatt tctcgattat tttctccaat
     5581 cctataattt ttttattcaa gtagagaaaa atatactaag attaaaggga ctgtgacaaa
     5641 aatcttgtca cagtcccttc ttccgaa
//
LOCUS       DAYYAR010000148         5615 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000148 DAYYAR010000000
VERSION     DAYYAR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.151"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..2231
                     /locus_tag="ACHAT0_002523"
                     /pseudo
     CDS             172..2231
                     /locus_tag="ACHAT0_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
     gene            2314..2883
                     /locus_tag="ACHAT0_002524"
     CDS             2314..2883
                     /locus_tag="ACHAT0_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8452427.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3058..3864)
                     /locus_tag="ACHAT0_002525"
     CDS             complement(3058..3864)
                     /locus_tag="ACHAT0_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452428.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4002..5516
                     /locus_tag="ACHAT0_002526"
     CDS             4002..5516
                     /locus_tag="ACHAT0_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8452429.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2003 a          969 c         1131 g         1512 t
ORIGIN      
        1 acaaaattaa aaagtacata agttattatt ccgattttct tgaaacaata aaaaatcaaa
       61 aaaaatactc tcctaatagt ccatcttctt ttgattttat ttatataatg aaatcatctt
      121 atgaaaatta ctacttcatg tttagacaag agtagggagg actaagaata aatgtgttca
      181 aaaagacgtt caaaacaact aaataaacac gcttgtcgtt tagataagga acggtcgtgt
      241 tctgaaaaac actacgttaa gtttatgatg cgcacgatgc tgatcagttt atctgtaggt
      301 gtctatatgc atgtacccgt tcaggcaacg acacaaacca tcaaggtcgt acgtacctct
      361 gtttctgaga ccactgattc ccctatcact attactcata ctttcactga tgcaaatgga
      421 aaacagctac ttcctgcatt gacttcctat aataaagtca gttatcctcc aacgataaac
      481 cactactggt taattcctaa caaaacatta tggacatcgg aaaccggaag cagtgtgccg
      541 ctttctatct tttccgatat gactgatcct acaaaaggtg atcagttaaa acagatcact
      601 gatattttga atcatgcttt gacagatata aaggacggtg gaagcgcaca agtttcttat
      661 gtatatgaaa aagattattc acagtttgaa agaaagaaaa tcgatgttct ggcagggaca
      721 aaagtttcga tatctgatct ggtagaaagg ctgaagaacg cagatggaac acaaggagat
      781 atgtccaaag taatttcaaa caatgggaat atcgtggata cgactaatac aggaacatct
      841 actatttcct tgtcctattt tgatccaatt gctttaaaaa caatgactgc atctgctgtg
      901 atccatgttc gtgctgatca aacagctatc aaaggaaaag atcttcactt aagaacagga
      961 gagagttacg cccctaaaga tcttgttgaa tatgtaacaa aaagcgatgg aacttcagga
     1021 aataaagaag aaatcaaaat gaacgggaca gtagatacta caactccggg agaatatccc
     1081 gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg cagaaacaac agctgtagtt
     1141 tatgtagaag catctgctgc ttcatcgact gtcatcactc attttataga tagtcaaaca
     1201 aataaagaac ttcaagcaga aacacatatg agtagtgtaa atcgtattcc tgatttgcgc
     1261 ctacctgctg aattttctga tcgagtgcca aatatagatt tatcgactta tgaacaaaat
     1321 gggaaagtca cttctatttc tgattggatg caagcaaacg gattagacag aaaaagtcac
     1381 tggtcggaga tccttgaagt acttcactca gaactgatga ataataggaa gcaatcagga
     1441 ggggaaatta cctttactta tgtttatata ccagacaatc gttcattaga aggtcatgat
     1501 cttattgttc atccaaacga aaaaataaac aaagaagagt tgatccaatc agcaacaaat
     1561 tccttaggta aacccgtacc gttaaaggat gtatcggtta caatcaatga agtaccattg
     1621 gaggatggtt atttgaccaa acaaacaggt gattttacga ttatgtatag ttatcatgac
     1681 ccgtattcac tccaagaact ccatgctcag tcactattac atgttttacc attaaacaaa
     1741 cagatagatc aaaaagaaca aaaaagacaa gaagaaagct tgtcagaaaa aaacaacaaa
     1801 tggctcatgc agtttctcca acagggaaag taaaacaaac aagaagccta gagttagcaa
     1861 gaatatctag aaaaatctca gaatctaata aaacgataag gcaagaagct catttggccc
     1921 cacaagtttc tggattggaa aatgaaattt tagagcctaa aataaaacaa caatcagaaa
     1981 aagaatttgt tgctaaacag tcggaagatc agaaggaatc agatgaccag aaggaaacga
     2041 agaaaaaacg cttgtcaact attgattcca ccgatgagca tctgcagccg caagtaccac
     2101 ttcctagtcc gggaacaccc gcacctggtg gaggtattcc ttcttctgct ttaggagatt
     2161 tcatgcgagg tatggcagga acatgggtat acgctaatcg aaatgaattt gacgcagatg
     2221 actggttctg atttatatct ctatctgtta tagtcaaata agtagctgag tcatatgtta
     2281 tttggcataa tggagacgag gagggataga aaaatgttag ataaaacgtt accttacgca
     2341 gaaatctgga tgacaagacc acttcaacaa aatgttccaa tcacgccttt agcggaaggt
     2401 tttgaatttc gattttatca aaaagaagat gaatctgctt gggcggagat tgagacttcc
     2461 gttggggaat ttcaaacgac agatgaagca cttgagtatt ttcaacaaaa ctttgctcct
     2521 tatcctgaag aacttgaaag aaggatgttt tttgtagaga caaaaaaagg agaaaaagta
     2581 gcaacctgta ccgcttggcg aaaaaaatca agagagcaag tgtatccggt ttttcattgg
     2641 ttagcagtga aaccagatta ccaaggaaaa gggctcgcaa aagcattgac tgccagagtc
     2701 ttgcaagaat ttcctaccct ccatacggac ggaccaatct atcttcacac acagacatgg
     2761 agccatcagg cgattgctct ttataaaaaa atgggtttta cttttattgc tgaaaatcta
     2821 gacggcacac ctaatccaga ttatgaaaaa gttatgcagg ttttaagtaa attagataaa
     2881 taacgaaatg gtgaaataag tcctcagtat tgggataaaa aacgaaatag tcattcctca
     2941 tatcttactt ttttgagtta atactatcgg ataactaatg gcatgccata cattcaaaaa
     3001 agaaggggca gtgacaaaag tattgtcact gccccttctc ttgtttttta gcatgtttta
     3061 tcgtttgatt tttttaggaa taaaattctt gatgaacgta aatttttggt aatcgtttcg
     3121 actatagagg taaccaaata cactgaaaac aactgctcca ataaaattaa caaataaatc
     3181 cttcatagta tccatgattc ccacgtctaa gtaaccattt tggatgattg ttttatctgt
     3241 tttaccatct gcattttgac tttcaatgat tgtccgagtg atatctggaa catgatagac
     3301 agtgtttccg tctttactta actccacact attgatttgc tggatgatcg tatctttttg
     3361 catatcgaat tgcagtaatt tatctgctga aaattccaca aactcccaca taacaccaac
     3421 tgtcatcgaa aaacagaagg aaacaactgc tagtaataaa ggagtcaagt tcatggtttt
     3481 tgtattttta ttgagcaaat aaacaaggga aaagccgaca cccccagcta aaaatccgct
     3541 gaatgcatgt agaaaagtat cccacattga aaaatagcca taaaaatcac ttaactctcc
     3601 taaaactgtt gaactaaaaa taaaagataa caaaatgatt tctagcatat ttggaatctg
     3661 tatctttaat atcttttcca gaaaataagg gatttcaaat aaaattaatg tcagtaataa
     3721 aagcattgta tttccccagt gttggttcca gacctgatcc actagcataa taacgacgat
     3781 gatacgtaat aaaatataga ttccgtttgt gattctacct gcagtttttg tactgttcgt
     3841 aaaaaaggtt ttcaaatgat tcataaaata actccttcat gtatatcctt aattatacta
     3901 agaagcgtgt gtttaccaaa gctatatgac ttaaagaaaa acagagttga tattgtgaaa
     3961 aaaatcactt aatatagtat aaggagaaaa gggggataca aatgaaaaaa ttagttgctg
     4021 gtttattaac agccgttgca cttcttgcta tgagcgcatg cggcgcagat cagaaaaata
     4081 cagcgcagac aagttctagt acgaaggaat ctgccgaagt atcatcaggc gcttctgaac
     4141 aagtatatac agatccaaaa gaaatgaaag aatcttatga tgtcgtgatt attggagctg
     4201 gtggtgcagg gatggcagca gctatcgaag caaaagatgc tgggttgaat ccagtaattt
     4261 tggaaaagat gcctgttgcc ggaggaaaca catctaaatc atcaagcggt atgaatgctt
     4321 cagaaacaaa atttcaaaaa gaagaaggaa tcaaagactc gaatgatgcc ttctatgaag
     4381 aaacgctaaa aggtggtgga ggaacgaatg ataaagaaat gcttcgatac tttgtcgatc
     4441 actctgctga tgcggtcgat tggctagatc aaaacggtat caaactggac aacttaacga
     4501 tcactggagg gatgagtgaa aagaggacac atcgtccgtc tgatggctcg gctattggtg
     4561 gatacttagt ggaggggtta ttgaataacg tccatgagcg ggaaatccct atttttgtca
     4621 acgcagatgt gacagacatt aagaaaaatg gggaaacggt caatgaagtg actgtacatg
     4681 ttcaaggaga agaaccaaaa gaggtcaccg gaaaagcaat cgtagttacc acaggcggtt
     4741 ttggtgccag caaagaattt attgaaaaat atcgtcccga tttagctgat tatattacga
     4801 caaatcaaga aggatcaact ggagatggta tcaccatgat tgaaaaatta ggtggacaaa
     4861 ccgttgatat ggataaaata caaattcatc caactgtgca gcaggatgag ggggtcttga
     4921 ttggtgaggt tgttcgcgga gaaggagcaa tcttggtcga tcaagagggg aaaagatttg
     4981 tcaatgaaat ggatacaagg gataaagtat ctgcagcaat tacagcattg cctgaaaaat
     5041 cagcttattt gatttttgac caaggcgtgc gtgatcgtgc cacagctatt gaattttatg
     5101 accaaaaagg atatgtcaca actggaaaga caatggaaga attagcagaa aaaatcgatt
     5161 taccaaaaga aacactagca aaaacagttg atggatggaa tacggatgtt gcggagaaaa
     5221 aagatgctca attcaaccgt gccacagcca tggaacatga cttatcgtct ccgaattatt
     5281 atgcgatcaa aatcgcacca ggaatccatt acacgatggg cggtgtgaaa atcaatacga
     5341 acactgaagt attagataaa gaaaaccagc caattagggg gttatatgct gctggtgaag
     5401 tcacaggtgg cttacatgga aacaaccgga tcggtggaaa ttcagttgcg gagatcatta
     5461 tttttgggcg acaagcgggc atacaagcag ctcgatttgc ttcggaaaat gaatgaaata
     5521 gaacgtaaat aaaaagaggt tgtgaaatca gcgttttgag ctaataccga gggtcagctg
     5581 attgaacact gtttagtgaa gaggttgtga aaaag
//
LOCUS       DAYYAR010000149         5613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000149 DAYYAR010000000
VERSION     DAYYAR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.152"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            187..2181
                     /gene="uvrB"
                     /locus_tag="ACHAT0_002527"
     CDS             187..2181
                     /gene="uvrB"
                     /locus_tag="ACHAT0_002527"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8452430.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            2191..5007
                     /gene="uvrA"
                     /locus_tag="ACHAT0_002528"
     CDS             2191..5007
                     /gene="uvrA"
                     /locus_tag="ACHAT0_002528"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8452431.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            5270..>5613
                     /locus_tag="ACHAT0_002529"
     CDS             5270..>5613
                     /locus_tag="ACHAT0_002529"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8452432.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPY"
BASE COUNT         1854 a          941 c         1286 g         1532 t
ORIGIN      
        1 cgaaaaaatg aaaaaaatgc gagtttctaa cattttatgt tataaaacat aacatgtata
       61 ataagataaa aaagtggcga aagtatgttc ggtgtggtat aattttttag taaggaatgc
      121 ctttgctttc cttttctttt tattggtcgt aaaatgaaaa ggaatgaatg aaaaggagga
      181 cgaagaatga ttgagagaga tacctcccga cactttgagc ttgtctcgaa ataccaaccg
      241 gctggtgatc agcctgaagc gattaaccaa ttagtggatg gcgtagtcgg cgggaaaaaa
      301 gcacaaatct tattaggtgc gactggaaca gggaaaacat atacgatttc caatcttatt
      361 gagaaagtga ataaaccaac actgattatt gcccataaca aaacattggc tggtcagctg
      421 tacggggaat tcaaggaatt tttccctaac aatgctgttg aatattttgt ttcttattat
      481 gattattatc agcctgaagc ttatgtgcct tcaagcgata cgtatatcga aaaagattca
      541 agtgtaaatg atgagatcga caaattacga cattcggcta ctagttcatt attagaaaga
      601 aatgatgtga tcgtcattgc atccgtttca tgtatttttg gattaggctc gccttttgag
      661 taccaaaaac aagtagtttc gattcgacag ggagcagaac tagatcgcaa tcagttgatt
      721 cgtgacttag tcagtatcca attcgaaaga aatgatatag attttcagcg cggtcgtttt
      781 cgagtgcgcg gtgatgtcgt ggagattttt ccggcttcaa gagatgaacg tgccttacgt
      841 gtggagtttt ttggagatga ggtcgaacgt atccgagaag tcaatgcact gacaggtgaa
      901 gttttaggag aaacggaaca cgtagcaatc ttcccggcga ctcactttgt gacgaatgat
      961 gaacatatgg agcatgccgt agcgaatatc aaagcggaat tagaacagcg tttaactgtc
     1021 ttgcgaaatg aaaacaaact tctggaagct caacgtttag aacagcgaac caattacgat
     1081 attgaaatga tgttagaaat gggctacacg tcgggaattg aaaattattc tcggcatatg
     1141 gacggacgta aagaaggtga accgccttat acgctattgg atttctttcc tgaagatttt
     1201 ttgatcgtgg cggacgaatc gcacgtaacc atgcctcaaa tcagaggaat gtataatggt
     1261 gaccgagcga gaaaacagat gctagtagat tacggtttcc gtttgccatc tgctttagac
     1321 aatcggccac ttcggttaga agagtttgaa aaacatgtca atcagatcat ttatgtctct
     1381 gcgacgccag gaccatatga acatgaacaa acagataccg ttatccaaca aatcatccgt
     1441 ccgacagggc tgcttgaccc agtgatcgaa gttcgtccaa tcatgggaca aatcgatgat
     1501 ctagtaggcg aaataaatga acgagtcgaa aaagaccagc gggtgtttgt aacgacattg
     1561 acgaaaaaaa tggcagaaga cctcacagat tatttcaaag aattagggat caaagtcaaa
     1621 tatttgcata gtgatatcaa aacgctggaa cgaacagaaa tcattcgaga tcttcgatta
     1681 ggtgaatttg atgtgttagt tggaatcaat ttgcttcgtg aaggattaga cgtacctgaa
     1741 gtttcccttg ttgctatctt ggatgcggat aaagaaggct ttttacgtag tgaacgttcc
     1801 ttagtccaaa cgattggacg agctgctcgg aatgaagaag gtaaagtaat catgtacgca
     1861 gacaaagtaa cagattctat gcgtctagcg atggatgaaa cgtcaagacg tcgtacgatt
     1921 cagcagaagt acaatgaaga acatgggatc gttccaaaaa cgatcattaa agaaatccga
     1981 gatttgattt ctattacgaa agaatcggaa gatgatacaa aagaagcggt acaagtctcg
     2041 tatgaagaaa tgacgaaaga agaaaaagat acgttattga tgaaattaga aaaagaaatg
     2101 aaaaatgcgg cgaaagcatt ggattttgaa acagccgcta atgtgcgaga tatgatttta
     2161 gaattaaaag cttcaaagta ggaggacaac atggctaacg ataaaattac gattcacggt
     2221 gcacgtgccc ataacttgaa aaatatagat gtaacgattc cacgagataa attcgtggtc
     2281 gttacaggtt tgtccggttc tggaaaaagt tccctagcat ttgatacgct gtacgctgaa
     2341 gggcagcgga gatacgtaga gagcctttct gcttatgctc gtcagttttt aggacaaatg
     2401 gataagccgg acgtagatag catcgacgga ttgagtccgg cgatttctat tgatcagaaa
     2461 acaacaagta aaaatcctcg gtcgacagta ggtactgtga cggaaatcaa tgactattta
     2521 cgcttgttgt ttgcacgtgt aggacatccg atttgtccca acgatcatat cgagattacc
     2581 agtcaatcag tagatcaaat ggcagatcaa gtaatggctt tgcctgagcg cacaaagatc
     2641 caagtgttgg caccgatcgt ggtaaagaaa aaaggacaac ataaaaaaat attcgaacga
     2701 atccaaaaag agggatacgt ccgcgttcga gtcgatggtg agatctatga tttaagtgaa
     2761 gtgcctgagc tggagaaaaa caaaaaacat gatatcgcga tcgtcatcga ccggatcatt
     2821 gtgaaagaag ggattcgttc tcgtctattt gattctttag aggcggctct gcgattggca
     2881 gacggctatg ctctggttga cgtgatcggt gaagaagaaa tgttgtttag cgaacactat
     2941 gcctgtcctt attgcggatt tactgttggc gaactagaac caagactgtt ctcattcaat
     3001 gcgccttttg gagcttgtcc agactgtgat ggtttaggtg tcaaattaga agtcgatcaa
     3061 gatttagtga ttccagatag aacgaagaca cttagtcaag gtgctatcgt tccatggaat
     3121 ccaatcagct ctcagtatta tcctcaaatg ttagcgcagg cttgtgaaag tttcggtatc
     3181 gatttagata caccattcga agaattaccg gaagagcatc aggatatcat cttgaatggg
     3241 acagatgatc ttttccattt ccattatgaa aatgattttg gtggcgttcg cgatgtagaa
     3301 acgactttcg aaggggtatt gaagaatatc gagcgtcgtt atcatgatac gaacagtgat
     3361 ttcacgcgtg agcaaatgcg tctttatatg acagaattaa cttgtcagac atgtaaaggt
     3421 tatcgattga atccccaagc tctatctgtt caaatcaatg gaacgaatat cgggcaaaca
     3481 aatgagttgt ctattcaaaa agcagtagat ttcttctcaa acttaacgtt atctgaacaa
     3541 gagaaagtaa ttgccaaacc gattttaaaa gaagtaaatg acagactttc attcttggaa
     3601 aatgtgggac tagactatct aactttgagt cgcgcttccg gaacattatc tgggggagaa
     3661 gcgcagcgaa tcagattggc tacacaaatt ggatcgaatc tttctggagt tttgtatatt
     3721 ttagatgaac catcgatcgg attgcatcaa cgcgacaacg atcgactgat tggctcatta
     3781 aagaaaatgc gtgatcttgg caacaccctc gttgtagtcg aacatgatga agatacgatg
     3841 tgggcagcag attatctgat tgacgtcgga cctggggccg gagaacaagg cggacaaatc
     3901 gtggctgcag gcactcctga agaagtagct aatgatgaaa attctttgac agggaaatat
     3961 ctttcaggga aaaaatcaat ccctgtacca aaagaacgcc gtaaaggtaa tgggaaagca
     4021 atcaagatca ctggtgcttc agaaaacaat ctgaaaaata ttagtgttga atttccatta
     4081 ggagaatttg tagcagtcac aggtgtgtct ggttctggaa agagtacatt ggtcaatagt
     4141 atcttaaaga aatcgttagc gcaaaaatta aataagaatt ctgctaagcc aggtaaattc
     4201 aagacaattt ccggctacga aagtatcgaa aagatcatcg atatcgatca aagcccaatc
     4261 ggccggacgc cgagaagtaa tccagcgact tatacaagtg tatttgatga tatccgtggg
     4321 ttatttgctc aaacgaacga ggcaaaaatg cggggttata agaaagggcg ttttagtttc
     4381 aacgtaaaag gcggtcgttg tgaagcttgt cgcggggatg gaattattaa gatcgaaatg
     4441 cactttttgc ctgatgtcta tgttccttgt gaagtatgtc atggcaaacg atataactct
     4501 gaaacattag aagtgcatta caaaggaaaa agcattgctg atattttgga aatgacagta
     4561 gaagatgctg tagaattctt caagcacatt ccaaagattc atcgcaaact gcaaacgatt
     4621 gttgatgttg gcttaggtta tgtgacgatg gggcaaccag caacgacatt gtccggtggt
     4681 gaggcacaac ggatgaaact tgccagtgaa ttgcacaaaa tctctaatgg aaagaatttc
     4741 tatatactag atgaaccaac gacaggactt catagcgatg acatcgcccg cttgttgcat
     4801 gtattacaaa gattagtaga tgctggtaac acagttttag tgattgaaca caatctagat
     4861 gtaatcaaaa cagcagatta tatcattgat ttaggaccag aaggtggaga aggtggagga
     4921 acgatccttg cgactggaac accagaagaa atcattaacg taaaagaaag ttatacaggt
     4981 cactatttga aaaaaataat ggtataaaga ggatagatag ctgagatagt taaaaaaaca
     5041 taaaaaaatg aatttttgct tgttttatta atatcagtgt tctagaatca ttacatggta
     5101 gttctttcta cactgattgg tatagacaaa aacttcctat aagttcaatc cgttcaaaaa
     5161 agaattgagc aatcggagga gtttcctttg attgcttttt tctttatggt atgatattcc
     5221 ttgtgtagtt ggaaaagaaa aacaagagat tatgagtaag tgagagataa tgatgaacag
     5281 tattgaaaat gaagaattta tccctatatt attaggaagc gacatgaacg tttatggtat
     5341 ggcacgttct tttaatgaag catacggcaa aatctgccaa gcatatgctt cggatcagtt
     5401 agcccctacc cgctacagta aaattgtcaa tgtagaagtt attccaggat ttgataaaga
     5461 ccctgtgttt atcgagacga tgcttcgttt ggcaaaagaa agatattctg ataagagtaa
     5521 aaaatatctt ttgattgctt gtggtgatgg atatgcagaa ttgatctccc aacataaaca
     5581 agagctgtca gaatatttta tctgtccata tat
//
LOCUS       DAYYAR010000150         5516 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000150 DAYYAR010000000
VERSION     DAYYAR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.153"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..502)
                     /locus_tag="ACHAT0_002530"
     CDS             complement(44..502)
                     /locus_tag="ACHAT0_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8452433.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(515..1846)
                     /locus_tag="ACHAT0_002531"
     CDS             complement(515..1846)
                     /locus_tag="ACHAT0_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8452434.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1857..3299)
                     /locus_tag="ACHAT0_002532"
     CDS             complement(1857..3299)
                     /locus_tag="ACHAT0_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8452435.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3286..4050)
                     /locus_tag="ACHAT0_002533"
     CDS             complement(3286..4050)
                     /locus_tag="ACHAT0_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8452436.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4154..5029)
                     /locus_tag="ACHAT0_002534"
     CDS             complement(4154..5029)
                     /locus_tag="ACHAT0_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8452437.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5284..5368)
                     /locus_tag="ACHAT0_002535"
     tRNA            complement(5284..5368)
                     /locus_tag="ACHAT0_002535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5332..5334),aa:Leu,seq:caa)
     gene            complement(5386..5457)
                     /locus_tag="ACHAT0_002536"
     tRNA            complement(5386..5457)
                     /locus_tag="ACHAT0_002536"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5423..5425),aa:Gln,seq:ttg)
BASE COUNT         1673 a         1175 c          831 g         1837 t
ORIGIN      
        1 ttcacccata aatatcaccc tatttaactt ttttcatcgt acactaaaat tcaaaacaac
       61 tatctttaat ctttacttct tggttttgtt ttaataaatt tccttcaaaa atgcctggag
      121 aaactacaat gatcaccgta tctttttgtg cttgctccaa cttcttcaaa tctataatag
      181 ctaacagtgt cccttctttt acctcctggt tactttcaac ccgtatctga aacggacttc
      241 cttcgagttc aactgtatct aaacccatat ggatcagtat ttttctgcca gattcacttt
      301 cgatcgttat ggcatgtcgg gtaggaaata tattatcgat tattccttta actggcgcat
      361 ggatatatcc atcatgattc gttatagcgt atccaactcc tatcattttt gaagaaaaga
      421 cctcgtcttt tactgctgtt aatggaataa tttgaccact agctacagct tttagaatgt
      481 ttcttgtttt aaagaaatgc actttttatc acctctactt taatgctggc caatattttt
      541 tgtttacttt gatcatttca tctagtattt tttttgcgac tctggcattt ggtacggttt
      601 gattcaatgt aaatgcttgt aaagcttttt gataagaacc ttcaaagtaa gcatctacta
      661 gtaacttctc tactgcaact tgtgcctcca tcaatccttt atgaaaatca ctgattggtt
      721 ttcttagtgt aattggctcc acaccagttg caccaacata tgccggtatt tcaacaactg
      781 catctgaccg tagattcgga atcgcccctt gattaggaac gattagcata aagcgacgac
      841 gatcatcatt caacaaggaa atagctatat cgacgatata ttgtccatgc tccccaaaat
      901 taaagttcaa tacttcttcc acaataccgc ggcgtatttt atcagccatt tctttggtat
      961 ttttctctcg tccatccatg accatattag cacgtgtata ctctttgtca gtatgctcaa
     1021 cgaccatatc aggatataag taatactcta agtaattatt cgggatatta cttggaaaat
     1081 ttttcgtgat gactgacatc atttgaaagg ttttttgcca agttttatct cctatattaa
     1141 aatcggcgac ttgcatttct tttgttatta gtttttcaat aatctctggc ataatttctc
     1201 gttgcaattc tttatcataa atcgatgtat accagccaaa gtggtttaaa ccataatacg
     1261 taacgatcca attcttacga tcataaccgt aattcactgc aatcgtttct tcaattgaaa
     1321 tagtcatatc acaggcatta atcattttta cattcggata tttacgacga acagcttcag
     1381 aaacgatcga ctctggattt gtataattca aaatccatgc tttgggggca tagtcttgta
     1441 tatgatcgac aatttccaat aaacctttga ttgagcgcat cccataagca aatcctccga
     1501 gtccacacgt ttcttgtcct actaatccat gtttaagtgg aatcttttcg tccttttctc
     1561 gcatctccaa tcctccaaca cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa
     1621 gctttggatc atccgttgca cgaatgcgaa tatttgaaaa accttttctt ttcagcataa
     1681 atttaacaat caagtacata tccatatttc tttcttcatc aatatcatat aaacgtattt
     1741 cagataatgg caaccgtttt tcattgttca aaatggcttg aacgattcct ggtgtatacg
     1801 tactcccgcc tccagctata gtgataatct gttttttcat catcatcttt tcctttctat
     1861 tcttctaata atttattgaa ttctgccata acataagtca cttctaagcc gatgataatt
     1921 tgataatttt tttcagttgg tttaatgatc ccgcttatcc ctaattgctt gattcgctgt
     1981 tcctcgacca tggtgggatc cactacctct acccgtaggc gtgtattaca actatagaca
     2041 tccaggatat tggcttttcc tccaaacgct tgtagcattt ttgttgccat atattcataa
     2101 tttttacttg ctaaccctaa ctctgtttct tctaaggtta cttcattgcc aaactctgcc
     2161 ttagcttcac gtcctaatgt ctgaatgttt ctcttattga tcaaataata aaaagtaacg
     2221 taatacaagg caaaaaagaa aattccgatt ggaagaatca aaatagcatt ggtagcgatt
     2281 cgaaaattga tgagataatc aacaatacat gctccgaatg taaaaccaat tcgtatattg
     2341 aataagtaac agacaatccc tgctaaaccg gcaaaaagtg catggatcac gtacaacata
     2401 ggagaaagaa acatgaaact aaattcaaca ggctcagtga tatttgcaat aaatgaagtt
     2461 cctgcaccgc ttcccatgat tcctttgacc atctctttat tttctttata agctgcttta
     2521 tagatagcta gacatatagc tggtatacca aacatcatta ctacaaaaaa aactgataaa
     2581 aacagtccag cctgaggatc tccgttgata aagcgtgtca tctctcctct gaaaacttcc
     2641 ttgctaggag atgtatagct tcctaaatcg taataaatat acgtattcaa cacatggtgc
     2701 agtccaaagg ggatcagcaa acgattcaaa aacataaata tccccacacc taatgctccc
     2761 atatcaacga gcagttttgc aaaattatca atcccaattt ggatgagcgg ccagataaag
     2821 cccaaaaaaa cagctgcgat tgtttgtaga atcatcacca tagtcaatgg gaatttttcg
     2881 cccgcaaaat aagaaaaaac tactggtaat ttttgttctt taaagcgatt atacgtccat
     2941 gccgctacta aaccggaaat aattccacca aaaatcccca tattcacttc tgggtccata
     3001 atttctaagc cttttttcaa ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct
     3061 ttgtccttat ttttgacaaa accgattgca actcctatag caaataagat atccaagttt
     3121 ccaaaaacgg catttccagc tgcaccaatt actgggatgt tcagcatatc gtcagctgat
     3181 aaacgcatga tcaatcccgc taatggcatc gctgcaactg gaagtagcat cgctcttcca
     3241 agcttctgca catttgattt aacattcatt tttattcctc ctctattaaa tatcctcata
     3301 tgttactaac tcaatcaaag gatcattttt cactttttga gcaaatgctg aatgaattaa
     3361 aaattcgact tggtgggtac gaggatacac aaaacttgaa ccatttaaca attcctgatc
     3421 caaatacgct gcgtgacaca tgacttcgat cgttttatct tctctcaata tcatgagtaa
     3481 acttgccaaa tattcttcca cactcgtatt gatttgtcgt tttttttcat cagtttctcc
     3541 tacgacatcg aaacaaggct caaaataatc gacatgacga acttcatctt ttcgttcaaa
     3601 gttgctccga accggcaaat catatttttt tgctaatgta acaactacct cctgattttc
     3661 atccaaagta tgagtatgat gatgactgtc taaatgagtt ggtatgatcc ccgcatgata
     3721 aattttttga atctgagcat tccattcctg atacagttga tctttgtcaa tgttaaatgg
     3781 atgctgataa taagataagg atctgaactc tccgctttcg ataagagtat cgacagtttt
     3841 gaggagtggt ttgccacacg tcaatgttaa atggactcct acacctagcc taggcatttc
     3901 ctttcttagt ttcacagcat gctcgaaacc cggcatattt gccatcaatg tagttgatgt
     3961 caaaattcct cgctgataag catccataat cccataattc acaccatggc tatagccaaa
     4021 atcatccgaa ttaaaaatca cttttcccat ttcattctcc ttcttttttc tactaaaata
     4081 ggcgcaacca aataatcata gtccatttac tatgtgtcat ttggttgcgc ctatagatag
     4141 ttacgccctt attttaatag attcttgcta gatgaattgc caaatagatg atttcatttt
     4201 cttgaatggc aagttgaaat tgctgttcga tgatatcttt aatctttagt gctgtacaat
     4261 acgctttgct atgtttaaat gtttcagtaa aaaaaccaat attttctact ggcgtttctt
     4321 cctgataaat caagcgttta accaggaatt tgatatgaac cattaagcga tgataagtca
     4381 gtgattcttt atcataaacg tttgaatagg atttttctag atgggacaaa atcgtggtcg
     4441 tcatttctac tatttttaag ctctttaaac tattaggctc tcctagctca gcattaatga
     4501 tatgcatagt gatgaatcct acttcatttg accctaactc ggtttgatat cgatgattga
     4561 ttcgctcgac cacttctact gctatatcat attcatttgg ataaaggaat ctcatttctg
     4621 gcaacataat tgctgtgaca tctctatctt ctctttttcg ttgaatagca aaataaatat
     4681 gatctgtcaa tgtaatatag atatttgaat ctaatggaga ggagatccta gctgttatca
     4741 gttcaatcac ttcttccgca aaatccataa attcgattgg aatctgcgag actatctcac
     4801 tgaatcgttt gatcttttct ttagaatcta atacaaataa tttgtcagca tcctcaacat
     4861 caaatggctt actcacttta gcagaaaagc caatcccttt ccccatcgca attttttcta
     4921 ttccatcgtc atcgatcaat acagcattat gattcagctt tttcatcaca tacaccgtta
     4981 attcacctcc tctatatgat aaaacaaaac aacttgcaga ctccttcata catatttttg
     5041 tctccaaaca atttttaaag acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg
     5101 cctaattgaa tagtagcacc ctacataatt tacaaaaata gtttaacaca taataaaagc
     5161 gttatcaata tcttttttac tcgttttatg ataatacttg tatcgaattg atttggaggt
     5221 aattacataa aaacaaaaaa aatatagcaa ctctctataa gaaagttgct atattgataa
     5281 ttataccggc ggccggggtc gaaccggcac gtcctcaacg gacactggat tttgagtcca
     5341 gcgcgtctgc caattccgcc acgccggcaa atttaaaaat aataactggg gtagctggat
     5401 tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta taccccaaga
     5461 ataaagggcg aatgatggga atcgaaccca cgaatgccgg agccacaatc cggtgc
//
LOCUS       DAYYAR010000151         5360 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000151 DAYYAR010000000
VERSION     DAYYAR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.154"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..786)
                     /locus_tag="ACHAT0_002537"
     CDS             complement(124..786)
                     /locus_tag="ACHAT0_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8452438.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            970..2616
                     /locus_tag="ACHAT0_002538"
     CDS             970..2616
                     /locus_tag="ACHAT0_002538"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8452439.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2607..3689
                     /gene="uxuA"
                     /locus_tag="ACHAT0_002539"
     CDS             2607..3689
                     /gene="uxuA"
                     /locus_tag="ACHAT0_002539"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8452440.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3797..5350)
                     /locus_tag="ACHAT0_002540"
     CDS             complement(3797..5350)
                     /locus_tag="ACHAT0_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452441.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
BASE COUNT         1734 a          904 c         1022 g         1700 t
ORIGIN      
        1 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
       61 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
      121 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
      181 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
      241 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
      301 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
      361 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
      421 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
      481 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
      541 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
      601 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
      661 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
      721 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
      781 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
      841 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
      901 catagcgatt tttattgtat aaaattcact tgaatacaaa aactaatatg ttagtttagg
      961 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
     1021 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
     1081 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
     1141 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
     1201 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
     1261 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
     1321 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
     1381 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
     1441 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
     1501 attcttgctg cgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
     1561 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
     1621 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
     1681 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
     1741 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
     1801 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
     1861 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
     1921 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
     1981 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
     2041 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
     2101 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
     2161 ccaaatattc ctgatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
     2221 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
     2281 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
     2341 ggaaaaccat tcatcccgag tccagaccca ctattgacag actttcagca taagctttcc
     2401 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
     2461 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
     2521 gaaatgttca tcaagatggt agcaggaccg aatgccgtac gaaaaacatt acaggattac
     2581 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
     2641 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
     2701 cattgatgga tataccagta ggagaggtat ggcccaggga gaggatcaaa gaactaaaag
     2761 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
     2821 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
     2881 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
     2941 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
     3001 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
     3061 aatttgaatt gccgggatgg gaacctgagc gcttagctca tatcaaatcc ttgcttgaag
     3121 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
     3181 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
     3241 actcgatttt tggattacct cgaatcgtaa agaatcgaga agatttagat tggttgtgcc
     3301 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
     3361 cagaaaatga tgtatatggg attttagcag aattttgtca aagagatcga atcccatttg
     3421 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccgcataaat
     3481 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
     3541 acgaatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
     3601 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
     3661 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
     3721 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
     3781 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
     3841 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
     3901 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
     3961 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
     4021 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
     4081 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
     4141 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
     4201 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaaca
     4261 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
     4321 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
     4381 caggtataaa caaatcaaat acagtacaaa aggactccac catttctctt gaataaagaa
     4441 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
     4501 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
     4561 ttggcagatt aggtcgcaaa atatctacag agggatcaat aaaaaagtca atatattcat
     4621 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
     4681 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
     4741 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
     4801 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
     4861 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtacaatg
     4921 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
     4981 gctgacttaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
     5041 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
     5101 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
     5161 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
     5221 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
     5281 gaaaaatatc taattttgta ttgctttttt tcttcaagag aaacgtttta atcgcttctt
     5341 gagtttgcat gaaagaggct
//
LOCUS       DAYYAR010000152         5343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000152 DAYYAR010000000
VERSION     DAYYAR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.155"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHAT0_002541"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHAT0_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHAT0_002542"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHAT0_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="ACHAT0_002543"
     CDS             2092..3132
                     /locus_tag="ACHAT0_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8452442.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /gene="traE"
                     /locus_tag="ACHAT0_002544"
     CDS             3956..4288
                     /gene="traE"
                     /locus_tag="ACHAT0_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8452443.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="ACHAT0_002545"
     CDS             4907..5248
                     /locus_tag="ACHAT0_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8452444.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1772 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     4861 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 ttt
//
LOCUS       DAYYAR010000153         5312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000153 DAYYAR010000000
VERSION     DAYYAR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.156"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHAT0_002546"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHAT0_002546"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8452445.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHAT0_002547"
     CDS             complement(1297..2133)
                     /locus_tag="ACHAT0_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452446.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHAT0_002548"
     CDS             complement(2317..3534)
                     /locus_tag="ACHAT0_002548"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8452447.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHAT0_002549"
     CDS             complement(3683..3844)
                     /locus_tag="ACHAT0_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452448.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHAT0_002550"
     tRNA            3917..4000
                     /locus_tag="ACHAT0_002550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5312)
                     /locus_tag="ACHAT0_002551"
                     /pseudo
     CDS             complement(4240..5312)
                     /locus_tag="ACHAT0_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DAYYAR010000154         5285 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000154 DAYYAR010000000
VERSION     DAYYAR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.157"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..234)
                     /locus_tag="ACHAT0_002552"
     CDS             complement(<1..234)
                     /locus_tag="ACHAT0_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452449.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSS"
     gene            complement(532..1506)
                     /gene="sgrA"
                     /locus_tag="ACHAT0_002553"
     CDS             complement(532..1506)
                     /gene="sgrA"
                     /locus_tag="ACHAT0_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8452450.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1702..2541)
                     /locus_tag="ACHAT0_002554"
     CDS             complement(1702..2541)
                     /locus_tag="ACHAT0_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8452451.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2726..3613
                     /locus_tag="ACHAT0_002555"
     CDS             2726..3613
                     /locus_tag="ACHAT0_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8452452.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLIDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4207..4902)
                     /locus_tag="ACHAT0_002556"
     CDS             complement(4207..4902)
                     /locus_tag="ACHAT0_002556"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM8452453.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4886..5284)
                     /locus_tag="ACHAT0_002557"
     CDS             complement(4886..5284)
                     /locus_tag="ACHAT0_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8452454.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1681 a         1066 c          777 g         1761 t
ORIGIN      
        1 agaagaaaac tcgactttta tcatcttgtt ttctgaaaga aaagataatt tatattctgg
       61 atacctttcg caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag ccagggtgta
      121 gtttagatct aaagcgatat ccttcaccac atataatttc tcgtctcttt cataacaata
      181 gttcaaaaat tgatatactt gcctttcatt gatatcaata aattcttcaa acaaagtaca
      241 tactcctttt tatctattat ttagacttat tccgacagct acatgaataa cgtacaaata
      301 gattcgttac tgatttttca gagatactac catgcgcagt gtgcattgtc aattattctt
      361 gctcttattt tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt tagtaaacaa
      421 aaatagatct ggcaaagcta caacttcgcc aggcaataca gcactctcga atgaaatatt
      481 cactcaaaaa tctatcgttt tattcaattt tagatctgtt gcttttattc tttatttcac
      541 tcgatggaag agagaacgaa aatagcttgc tcctacaatc aaaatgagta acacacccat
      601 ccatgtaatt agcttgttca aggttctact accagtttct ggtaaatcgc tttttctatt
      661 atctgtttct ttcgcttttt cttgatgatc aattccacga gaaacaaaat caggctgttt
      721 tagcacactt tttaggttgc tccctgcatt tctcttttgt tccttagttg gtatgatagg
      781 taccaaatca agttcattat ttacttttcc tgtgctgctt tcgctgctcg tgcttcctgt
      841 gctactctca ctactcatgc ttcctgtgct actttcacta ctcgtactct ccgtactgct
      901 ttcgctgctc gtgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
      961 gctcgtgttt tctgtgctac tttcactgct cgtactctct gtactgcttt caccgctcgt
     1021 gttttctgta ctgctttcac tacttgtgct atccgtactg cttggcggtc caacaactgt
     1081 ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa aaacaatcaa
     1141 atcaacttta tctttactct tgatctcatc aatcgatagt tctgcagaaa tcattattgt
     1201 atactcaata ccagcggtcc attgtacgtc ttttaaataa acttccccaa taactttttc
     1261 tgaacttcca ttaccatccc aaccatatgt tagaacagca tgtacatcac tagcattcac
     1321 gcttttgttt ggtttgactg gaatagtaat gacttgttgt gtcatgtcat aaacaatccg
     1381 atcagttttc acagtaaatg aaagattctc atttgcccgt tcatttgcag aagtactgat
     1441 tccaatcatt gctgcaatta tactcagccc aagaataaca aataatacgg tcgctgtttt
     1501 cttcatttct atcactctcc atataattac cttctatttg ataaaggaat tatataaaat
     1561 gaaaatgatg agaacacgac agtcagttgt gaaaatctaa caaaagatgt cgtgtattcg
     1621 cttgaatttg tttacttttt cgtttaatca atgacagact taaaaaagca agaccgatat
     1681 cagccttgct ttcttcatct tttattgata aacagcagtg tatttcttca tccgctttgt
     1741 tttatatgca tagagtaaca aaccaacaat caagactaac aagaataatg tagttgatat
     1801 agatgcttca gccccaaagt ttcccccagt aattattgta ttttgcgatt ggatcagagt
     1861 ggagaaaaaa ccttgttctt gcgttccgct gactggtata cccaaaatag ctccttgtgt
     1921 ccaattccaa gtaatatgag caccaatagc cgtaaataaa cttcctgtac tgtagataag
     1981 tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact gccagaaatg tcatgttagg
     2041 attcaatccg tgcaaaagag caaataatgc agcagatcca atcattgcta ttaacggacc
     2101 aaatctaagc atctgatgct gaaaaaagcc acggaataaa agctcttctg atgcccccac
     2161 tgcaataaat aaaataaaac tcgttatcag cggcacaatg tttgcgtttc ctgaatctaa
     2221 ccgatacgcc cccgtcaaat ataatggact agtaataagc agaaacactc caattccaac
     2281 aacgagacct cccgctaaat acccccagtc ttttcctcga acagaaacac cataatcttc
     2341 tattcttttt ccttcgacct tactagcaag aaataaagct agtaaaaaaa cagctaaatg
     2401 tccgaccgta ccggcaattg ctccaatcca ttcactattt ccaataaaca tatccgcaag
     2461 tgcattattg acaagaattc ctaaaacaaa accaactaag actattaaaa cagatccaat
     2521 caaccgaata atcctgatca tctttcatcc ctcctttata ttaagtttaa caaccctttt
     2581 ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca tagagagaat agatttttac
     2641 gaactaagaa tatctacagc aaccttacga aaatgaaaga ctgtctccct aattcttctg
     2701 ttagactgtt tttaataagg aaaggatggg aaattttttg aaaaaacaac tactaattat
     2761 gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca atgaaacgat
     2821 tgcaacgttt aaaggtggaa cacttacagt tcaggatttt tatgataacc caaaagttaa
     2881 atcaaataat caaacactat taaaaaaaat gattgtcttc aaagcatttg atgatgtata
     2941 cggaaaagag atttcttcaa aggaagtaac aaaagaatat aatgaacaaa tcaaaaaact
     3001 cggtccaaac tacaaggaac aattaaaagc agttggccaa acagaagaga catataagaa
     3061 actctttaaa cagatgctgg ctttccaata cggtttgaaa gccaatgtaa aattaataga
     3121 taaagatcta gacactgctt ggaaagaatt ctatccagaa gttagtacac agatcattct
     3181 attttcgact aaagaagaag cagataaagc aaaaaaagaa gcaaatgaag gagaaaattt
     3241 ctccaagctt gttcaagctt atggaaaaaa taaaacatta aaggaaactg atggaaaaat
     3301 gaattttgat tcaaccaatc cagagattcc tactgaagta aaaaaagcag catttaaact
     3361 aaaaaacggg gaagtttctg atatcattcc tgtaacagat ccaacgactt atcagcaaag
     3421 ctattatcta gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag acaaatacaa
     3481 atctgagcta gaaaaaattg ctaccgaagc aaaaactctt gatacggaat tcatggataa
     3541 aacaatccgt aaagtgatga aaaaagacaa tgtaactatt aaagatcctt atgtaaaaaa
     3601 tatattcaaa taaacaaatt tgacccaatt ggggatgtgg caaaatttga gaagtgattt
     3661 ttggaaattc cttcttattt ttgaggcaaa ccacttttgc cacaacttca tcggggcctt
     3721 tttttatttg taccaaaaca aatcactgga tttgttacaa tcatgaggat ataatttaaa
     3781 aaagtttttt atttctatca catgaatcaa tgcgccatta agtagattct ttcatgcttc
     3841 cctatttttt cacttgccgt atcgcttcaa aaacaactat cataaactca aactcgactg
     3901 aaaagttatt ttaaaaaaga ctaaaaatat cccttctgga cttgttcttc ttattctgtt
     3961 atactagaga aacacgaaag atatttttat gcgacacttt tcatttgttt ttatgaaatt
     4021 tattcaaaac tggcgtaact tcagagttac aaggatgtat tagaggttag ggaatacatc
     4081 gtttaaatga gaaataagta atagttagta atagtcaccc aaactttaaa ttaatgaata
     4141 ttgaatcgtc agatagcctc ctcgagtttt cgagaaggct atcgtttatt ttatggagaa
     4201 attgattcag aagtaatcag cgaccttttt tagttcacca atcgtttctg cttggaaagt
     4261 ttgcacccaa tcttcttgga agcccctcac tgctttatca atcgagggca tctctaatac
     4321 ttgatctagt agctctttac ctaacgttat cgcatgagct ccagatcgaa tggctgtagt
     4381 gacttgttca caatttttga aactagctgc taatattttt gttggtcttt gcgtacgttc
     4441 gatctcagtc cgcaacgaat caatcacttt catagcatca atgccttgat tttgcatcct
     4501 gttataataa ggtgcaatat aatctgctcc attattaata gctaaatacc cttgtaattc
     4561 tgtgtaaata gctgtaccgg taatcaaata accttctttt ttcaactttt tgatcgcttg
     4621 aaggcctgct tgattcaccg gcaccttgat aaacgtatct tttcccagtt cttctatgat
     4681 aactcgagca tcctctatca tttcttcttc cgttcttcct attgtctgaa tatggatagg
     4741 aatctgtccg attatttttt tgatttcttt catatgcgag aagaagtttg acgctccttc
     4801 ttttttcaga attgtaggat ttgttgttac gcctgttaaa ttcaaaaact gttgacaaga
     4861 ataaatactt tttgtatttg ctgtatcaag catgatttcc atatagcttt cccccattct
     4921 gatcctcttg atcgatttta atgattgtcc cctttcttaa atgcggcatt tccttatctt
     4981 ctacatacat tgttcctgga agttccggct catgagatcc atcaaatttt atcgtgatat
     5041 gtcctaattc attcaaatta gtttggacaa catctcctac tgctgtgatt acatacattt
     5101 gtttatcaaa acagagcttc atatcacaag ttatttcttt tcttacctga ttcaattcaa
     5161 tcttataaca ataatctgtt aatgtttccg gtgcttcatc tccaaataaa atcattattt
     5221 tttctttcct gaatacttct gcttctatac caacatctgt tacaaccgtt tcaaagatcg
     5281 tcatc
//
LOCUS       DAYYAR010000155         5249 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000155 DAYYAR010000000
VERSION     DAYYAR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.158"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHAT0_002558"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHAT0_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8452455.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHAT0_002559"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHAT0_002559"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8452456.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACHAT0_002560"
     CDS             complement(2169..2906)
                     /locus_tag="ACHAT0_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8452457.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACHAT0_002561"
     CDS             complement(2997..3524)
                     /locus_tag="ACHAT0_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452458.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACHAT0_002562"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACHAT0_002562"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8452459.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACHAT0_002563"
     CDS             complement(4401..4886)
                     /locus_tag="ACHAT0_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8452460.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACHAT0_002564"
     CDS             complement(5039..5236)
                     /locus_tag="ACHAT0_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452461.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
BASE COUNT         1394 a         1168 c          889 g         1798 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctca
//
LOCUS       DAYYAR010000156         5234 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000156 DAYYAR010000000
VERSION     DAYYAR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.159"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2241
                     /locus_tag="ACHAT0_002565"
     CDS             <1..2241
                     /locus_tag="ACHAT0_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM8452462.1"
                     /translation="KYLHPIMISIILIFTFLGSLSSIYVNVYTETSNFANQTTEETNS
                     PPYSEIQSIESTNQSSNNETTSTTSSTQLIDPTEKEITAAKAAGEKAYSETGRSQTFS
                     TVAATANFNGHTIPDGYTVIYAKDGVIVAVNGSMSADKTALQGVTKAIAFIDNRPDIH
                     NLFIGSTVNGTTITFPKSIYMGVATAGNTIDSVTPNADKSYSFTPSTWGGLPAGGGMS
                     TFSIGIQLANDIPFTGYSIKNGQIVDGKGNVSNFPTYQAATVQVNANRTGTFSYTVNG
                     GAAKIGTYGTTLSGIHVGDKVVITPTAVTGYSFAQTNGSFTALESNVDTVTYTGKQTP
                     ITIKYQDYFGQMIAPSKIMNLTYGSAAQDLTTNTPTINGYTFTAVSATETKNQSAMSV
                     LASLDTNGNVIVKDANGKQISEVILYYKTNATVSINANGSKYYDGLSVVPIVKYAFND
                     KTESTSLLNNDLSIDQITWNPTDFKAMNEKGVEVTAPTEIGTYTSWQLTKSGLAKLAA
                     RTNYLFTIVQTSDVYTIKQISGAVELSGSKTYDGKAGVPSIHVKLAEGVTSSLTPVQV
                     ALSSMDYTADAQSAKNMVNAGSYTIKLTNSGIDKVKAANPKLSFTDIANTSGTYTIYK
                     ANAVITVDDAAFNYDAQSHSISVGNLHVTGVVLGESLDYTLTVNSRTDIGSQIVTISL
                     GEGSVNNNYTIIATDTAKLTINPTLLLPSTGGIGLLPFVFLGLIFIFSGFFYFIHRKK
                     KAGEQR"
     gene            2238..3152
                     /locus_tag="ACHAT0_002566"
                     /pseudo
     CDS             2238..3152
                     /locus_tag="ACHAT0_002566"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3225..3740
                     /locus_tag="ACHAT0_002567"
     CDS             3225..3740
                     /locus_tag="ACHAT0_002567"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8452463.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3877..5166
                     /locus_tag="ACHAT0_002568"
     CDS             3877..5166
                     /locus_tag="ACHAT0_002568"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8452464.1"
                     /translation="MKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKD
                     TAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLPF
                     YNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQFVT
                     GFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQKET
                     TFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHFIKQ
                     DVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKLISD
                     SEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQPKG
                     ILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
BASE COUNT         1847 a         1015 c         1040 g         1332 t
ORIGIN      
        1 aaatacttac atcccattat gatttcgatc atcttgattt ttactttctt agggagctta
       61 tcaagtattt atgtaaacgt ttatacagaa acgtcaaatt ttgctaacca aacgacagag
      121 gagacaaata gtccacccta ttctgaaata cagtccatag aaagcaccaa tcagtctagc
      181 aacaacgaga caacctcaac cacatcctcg actcaactga ttgatccgac agaaaaagaa
      241 atcacagcag caaaagcagc aggggagaaa gcttattcag aaacaggtag aagccagaca
      301 tttagtactg ttgcggccac tgcgaatttc aacggacata ccataccaga tggatataca
      361 gtgatatatg ccaaagatgg agtcattgtt gcggttaacg gatcgatgag tgcggataaa
      421 actgcgttac aaggcgtaac caaagcaatc gcttttattg ataaccgtcc agatatccat
      481 aatctgttta ttggctccac agtaaatggt actactatca cattcccaaa aagtatctat
      541 atgggggtgg ctaccgctgg aaatacgatc gattcagtta cacccaatgc ggataaaagt
      601 tattctttta caccctcaac atggggaggg cttcctgccg gaggaggtat gagtactttt
      661 tcaatcggta tacagttagc aaatgatatt ccattcacgg ggtactcgat caaaaatggg
      721 cagatcgttg atggtaaggg aaatgtttca aattttccaa cctatcaagc agcaacggtt
      781 caggtcaatg cgaatcggac aggaactttt tcttatacag tcaatggtgg agcagcaaaa
      841 atcggaacgt acgggactac attatctgga atccatgtgg gtgacaaagt ggttatcact
      901 ccaacagcag taacgggcta ttcttttgca caaacaaacg gttctttcac agccctagaa
      961 agtaacgtcg atacggtgac ctatacaggc aaacaaacac caatcactat taagtatcaa
     1021 gactattttg gacagatgat tgctccttct aaaataatga atcttactta cggaagtgct
     1081 gcacaagacc tgacgactaa tacgcctact atcaacggat atacctttac agctgtttca
     1141 gcgacagaaa caaagaacca atcagcaatg agtgttttag ctagtttaga tacaaatgga
     1201 aatgtaatcg taaaggacgc aaatggaaaa cagatttctg aagtcatcct atattataag
     1261 acaaatgcaa cagtatcaat caatgcaaat ggcagtaagt actatgatgg tctttctgtt
     1321 gtaccgatcg ttaaatatgc ctttaatgat aaaacagaat caacatccct tttaaacaat
     1381 gatctcagta ttgatcaaat cacatggaat ccaactgatt tcaaagcgat gaatgaaaag
     1441 ggagttgaag taactgcgcc tactgaaatt ggaacctata cgtcttggca attgacaaaa
     1501 agcggactag ctaaactagc tgccagaaca aactatttat ttactatcgt gcagacatct
     1561 gatgtctata cgattaagca aatcagcggt gcggtagagc tcagtggcag taagacatat
     1621 gatggaaaag caggtgtccc gtcaatccat gtcaagctag cagagggtgt cactagttcg
     1681 cttactccag tacaagtggc acttagcagt atggattaca cagcagatgc acaatcagca
     1741 aaaaatatgg ttaatgctgg cagctataca attaaactaa cgaatagcgg aatagacaaa
     1801 gtcaaagcag caaatcccaa gttgtcattt actgatatag ccaacacatc aggaacttat
     1861 accatctata aagctaacgc agtgattact gtcgatgacg ctgcctttaa ttatgatgcc
     1921 caaagtcact cgatttcggt tggaaatctc cacgttaccg gtgttgtctt aggcgaatca
     1981 ttggattata ccctaacagt taacagccgg acagatattg gatcacaaat agtaacgatt
     2041 agtttaggcg aaggaagtgt aaacaataat tatacgatta tcgcaacaga taccgctaag
     2101 ttgacgatca atccgactct cttgcttcct tcgacaggtg ggataggtct actgcccttt
     2161 gtctttttag gactgatctt tattttttct ggcttctttt attttatcca tcgtaagaaa
     2221 aaagcaggtg aacagcgatg aaaaaaacaa aaaatgtacg tgtggatata tttgctgttc
     2281 tcttttttat cctactggtc ttttttatag gttctccgac tgtgtcagct agagaatcac
     2341 ctattacatt tactttgcat aatcgaatgt tcgaaagcga taagatgcca agtatcagtc
     2401 aaaacgacgg aataaatatc ccggataact ttcaaggagt aaaagggttg aacggcgtta
     2461 ctttcgctat atatgatgtc tcggatgaat tttacaagct gcggtcagaa ggaagtagtg
     2521 tggaagacgc acaaagaaaa ctagcacaaa aaagtgatag tgaaaaaata ctggcagaaa
     2581 atgtgacaaa aacagtagac gaggaagagg gcattgcttc ttttagtgca tcggacaaag
     2641 atgagcaagg tcgtgacgct gtttaccgat ttgcggaaat aaagacttca gattaagtaa
     2701 aagaaaaatc agcaccattt gtcgttgtgc ttcctgtgac agattcatct aatcataaat
     2761 tgacgaccat ccacttgtat ccaaagagcg aacaaaaaat acctgctgca ctcacattaa
     2821 caaaaacagt agagaataag cagacagatt ttgcagatgg ggataaagtt ccttatctca
     2881 tcaccgtaac aattcctgag aacatcaacg agataggaac ctatacgatc aaagatacag
     2941 ctgatcctca gttatggcta gaaccagaaa cgatcgattt tttgattggt ggagacaaga
     3001 ttcatacatt tcacacacaa aaaacaaaac atggttttgt cttatcagat tctggaaaag
     3061 atcttagttc gtttgctggg aaaaaattaa cgatccgtta ccaaatgaca ttgaaagaaa
     3121 acagtgaaaa aacgacattt gataacgatg tacgtttgac gactgacaat caaacagtag
     3181 aaacacatct tgccatccaa acaggaggca aacaatttgt caaagtggat cggcaaacga
     3241 aacaaaaact agcagatgct caatttctag tcaaaaaaaa tgaccaatac ttagcgcaag
     3301 aaaacgggat aaatcattgg gtagccaaag atgattcaag agcaacgatt tttacttcca
     3361 gtaaagatgg aacattttct gtccatggat tgtcttttgg aagttacgaa ctagtggaga
     3421 ttaaaccgcc taaaggatat atattgagtc aatcaaccat tctctttgaa gttgaaaaag
     3481 gcagttatga tccgcttcat cctataccat tggaaatcat caacgaacca aaagcaaatc
     3541 cgacgaataa tggaaaaacg ccagttcaga tgcagacaaa tggtccatca aatgaaaaaa
     3601 atggaggaag ctttccaaaa acaagcgaag agatgcttgg aggtttctca attttagggt
     3661 tgttgctagt cctttcaaca gggacagctt ggttttataa aaaacaacaa aaaggaaaca
     3721 ataggaggga aataaaatga aaaacatcaa aacaaaaata tggacgtttt taggaactgc
     3781 tatcatgctg cttcctttcg ttctcggcct aggaactgca gaggtttcta ggccgtatca
     3841 cctacaccag aaaatgttac ggttaatttg cataaattga aattcacttc agcaccagaa
     3901 aatcaaataa acaacggaac ggaattgaca tttccgaata gcgaaccgtt gaacggcgtt
     3961 gaattcaacg tatatgacat cactgctacg tactatccaa gtaaagacac agcagttcca
     4021 gcagacgcta cacccttcgc aagtgtaacg acttctggtg aaggattagc taacctgact
     4081 cttccaggta aatctgatgg gaaagatgct gtatacgtct ttgtagaaac accaaagccc
     4141 ggtgtcgaaa cgtcgccaaa tatcgtgtta agcttaccct tttataaccc gaattcatca
     4201 gaccctgaca aacctttaga cacgattcac ctatatgcta aaaatgtcat caaagaaagc
     4261 aatatcaaaa tccagaaagc tgggaacttt tcaggggtag ataagttagc aggggcgcaa
     4321 ttccagatca aaaattcaga gaatcaattc gtgaccggtt ttgatgaaaa cactggtgtc
     4381 gctctctatg atcctgctga gtcagcgttg acattcacaa ctgatactga aggatccatc
     4441 agtgtcaatg gattaataga cggcaattat actttagttg aaacaaaagc accagatggt
     4501 tatcaattaa ccaatccaca aaaagaaact acttttatag taaaaaacgg aaaattgcaa
     4561 caaacaaccg gtatcatcca tggcgcagat gaagccaatc ttgggaatcc aaacggtgta
     4621 gtcaatcggg aaaatacaac tacggataac acgattactg tagaaaatgt ccaaaactac
     4681 ggtgatttcc acttcatcaa acaagatgtg aacacgcaag aaaaattagc cgatgcagaa
     4741 ttttctattc taagagcaaa agatccaact gttaaattaa tgaaaaaaat agcaaatctt
     4801 gcctcaggag aatacccata tgcttggtca gatgaagtca caggttctga ctgggaagaa
     4861 gtgaaattga tttctgattc agaagggaaa ttttcaatcg aagggctgaa acaaggggca
     4921 tactgccttc aagaaacaaa agcaccagaa ggctacgcat tacctaagaa tgaagctgcg
     4981 tacactgaat ttacagtaga tgatgatcca acaacttctg atgaaacaac gatcgataac
     5041 caacctaaag gaatcttgcc ttcaactggt ggtatgggga tcatcgtatt catcttggta
     5101 ggtactgctt tagttggagg agcagtgatc tacttcaaaa aacgccatca agaaacagaa
     5161 gcttaaacta ttggaaacaa aaatcatacg ataaataaac gatagtgatt caaatgggga
     5221 gaaaggttgt gaca
//
LOCUS       DAYYAR010000157         5199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000157 DAYYAR010000000
VERSION     DAYYAR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.160"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACHAT0_002569"
     CDS             25..696
                     /locus_tag="ACHAT0_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8452465.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACHAT0_002570"
     CDS             755..1195
                     /locus_tag="ACHAT0_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452466.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACHAT0_002571"
     CDS             1207..1875
                     /locus_tag="ACHAT0_002571"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8452467.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACHAT0_002572"
     CDS             1939..2562
                     /locus_tag="ACHAT0_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8452468.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACHAT0_002573"
     CDS             2618..3004
                     /locus_tag="ACHAT0_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8452469.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACHAT0_002574"
     CDS             3217..4608
                     /locus_tag="ACHAT0_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8452470.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1728 a          861 c         1095 g         1515 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacag
//
LOCUS       DAYYAR010000158         5123 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000158 DAYYAR010000000
VERSION     DAYYAR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.161"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..483)
                     /locus_tag="ACHAT0_002575"
     CDS             complement(<1..483)
                     /locus_tag="ACHAT0_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8452471.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     K"
     gene            complement(494..1162)
                     /locus_tag="ACHAT0_002576"
     CDS             complement(494..1162)
                     /locus_tag="ACHAT0_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFM8452472.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1293..1877)
                     /locus_tag="ACHAT0_002577"
     CDS             complement(1293..1877)
                     /locus_tag="ACHAT0_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8452473.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2212..2442)
                     /locus_tag="ACHAT0_002578"
     CDS             complement(2212..2442)
                     /locus_tag="ACHAT0_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8452474.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(2457..3407)
                     /locus_tag="ACHAT0_002579"
     CDS             complement(2457..3407)
                     /locus_tag="ACHAT0_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8452475.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3404..4246)
                     /locus_tag="ACHAT0_002580"
     CDS             complement(3404..4246)
                     /locus_tag="ACHAT0_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8452476.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(4379..5104)
                     /locus_tag="ACHAT0_002581"
     CDS             complement(4379..5104)
                     /locus_tag="ACHAT0_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8452477.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
BASE COUNT         1728 a          930 c          642 g         1823 t
ORIGIN      
        1 tttagagttt agcgataaac tacatccaat agaaattttc tgccgttcac cacttgatag
       61 cgaaaaaatg tttttatcta atatatttga aagatctaat aattcagcta attcattgat
      121 tcttttatcc atatgctctt ttgtaaatcc aaagttttcc atcgaaaata ctaactcact
      181 cgttgtattt gttgtaaaga attgtcctct aggatcttga cttacgtagc caatattttt
      241 tgatatactt tcttgtgtta aggattgtag cgagttattt tcaaataaaa tttccccaac
      301 caattttccg tcataaagtc ttggaataag tccattcatt aactttaata gtgtggattt
      361 cccactccca gaagaaccag taatcaatgt aagactacct ttttttattg aaaaattaag
      421 gtcaacaagc gcttgttcct taacatcctt atatgaaaaa gaaacattgt taaattcaaa
      481 caattctttc acctcaaatc aaaatactta agaaaaaaat agtaacggaa aacaggatga
      541 tatagtctaa taagctcatg tttgttttat agtaagaact tcttttcaca ggagattcta
      601 tcccctttgt tattgcatta gcggtcattt cttcagcaat catagttgca cgtataagaa
      661 cgggaactaa cagaaattcc aaatattgca gaggctgaaa aaaagacaag cgaatgtttc
      721 tcatttttaa agattcttta atcattctta tttcagaacg ataagtcgga aagaatctga
      781 tcactactgt aatacttaac attatttttt tcggtaaatg tattttttct aaagaggata
      841 ataattcatt gctagtatag acactaacca acaaccatga gatcattacg accggcaaca
      901 ttcgaaatac aatatataag gatgtcccaa aaaatatagt aaaaaactct atcttgatag
      961 catcaataat tttcattact aaaaacagaa ctgaataaat caagatgaaa taaatacatg
     1021 tctttatttt acgaaacatc aatactaaaa ctgtagcaat aatgaaacac cccacttgaa
     1081 atatatttcc ctttcctaaa aaaataatga ccggaaaaaa aatatttaaa gtaatcaagg
     1141 ttaacggatt gatttttgac actctttcac ctccaaaaaa taagatcgca ctgttatctt
     1201 aagctgataa aaatgcaccg ttattcagat aacagtgcga tcttatcaaa aagcataact
     1261 gagaacaatt ctcaatgcaa gctttttttt aattaactca gtaattcaat ccacccagga
     1321 acagaaaatt gatacaatct tctggttaat agcatagtct tttcttttga atagctatgc
     1381 ttcaatatat catagaatgt agcgtagaat gagtaaatgt agaagtggat ttcttcttca
     1441 ttaaagacta aattttgatt gatcattccg atagtcttta acttatctag acaacagact
     1501 gtcttttttg tcacaaatga tgtaattttt tcaacaatat cttgatattt ggttccagct
     1561 gattggaata aaaccaaatc gaatatacgt ttattctcaa aaatataatt aataaatgga
     1621 tctaattcat tgaagttctc tctccaactt ttctctatat ctatattttc taagtttaag
     1681 aggtatttgg taaactcttc attatacata ttaataagcg cattaatatg cggctgaata
     1741 atttcttcaa ataacgcttc cttagaagta aaatgtttat aaaaagcacc cgtagttacg
     1801 cctgcggtca agcacagttg ccttatctta gtttgctgaa atcctacttt ttcaaaatgg
     1861 cttagtccac tttctattaa tttttggtga gttgtaatat aatctctggt ttctatgggt
     1921 acttcctcct aataagatga attaactata ttatcttaca caggacttga agtttcaatc
     1981 gttaacgtct attactgaac tcattcctta gagctaaact aatgagaaag agcaaaataa
     2041 ctgatacaaa aagcaatcct acaagaatta atagactcat ctaaatcatt caccttttct
     2101 aaaataatcc gagaaagatt taaaaaaatt atatccagct attaggataa gtcctatcat
     2161 aatcactcca atagccattt tcttatcctc cctaaaaata aagcctttta cttaaaacct
     2221 ttttttgtaa tcgtcaataa attttttccc tttatcagtt aattgaaatc ctatatctaa
     2281 atagtcctca tatctaagtt tagtcagcat ctcacttaca gatgctggac taacacttag
     2341 aacttttgct aaatcattat tttttaattt cttttttctt tttgataatt cctgaattgc
     2401 tagtaaataa attagtatag tagttgtttt aatttgtacc atacctaccc tccaaattat
     2461 ttcgaaaggc cttcagcaat tttatccagg ttccatttca tcatcgcata gtaactatca
     2521 ccatcttgtc ctttttctgc aattgaatca gtaaaaatcg ttgaatagat aggaatattg
     2581 gtatcttttg atactgtttt cattggtcta tcgtccacac tgctttctac gaataaggag
     2641 ggaacttttg ttgtgcgtaa tttttcaact aagtgtttta tttgatctgg tgttccttct
     2701 tcttcagtgt tgatttccca aatgtaagca gaaggcacat tatacgcttt cgagaaatat
     2761 ttaaagcatc cttcacttgt aacaatcatt tttttatcat tcggaattga agcaaattta
     2821 gatttagctt ctttgtctag agaatccaat ttttcaatat acttatctag attttcttta
     2881 tagaattttt tattatcagg atctttttcc gctaactgtt tttcaatatt tttagcgtaa
     2941 ataataccgt tttctaaatt taaccaagca tggggatctt ccttcccttt ctcactctga
     3001 ccctctaagt aaataacatc tatgccatca cttgctgcaa aatagtcttt gttttcctct
     3061 ttgttcgcat tttttactaa ttttgtaaac caagcatttc ctccagtttc caagttaaca
     3121 ccgttataaa aaatcaaatc tgcttttgaa gttttttgaa catcttcagg caaaggttca
     3181 tattcgtggg gatctttccc aataggtacg atactgtgta aatcaatttt atcttttgct
     3241 atattttcag taatatctgc taaaatcgaa ttagtaacta ctacctttaa tttttcgttc
     3301 gaagccactg tatctttgtc tttactattt gttgaacatg ccccgaatat aaaaagagca
     3361 atcaaaatcc ccagacttcc cagtactgct ttccattttc tcatcataac tttctctcct
     3421 tctttcttaa aaatttttgt tttggtgaga ttagaaaact aattacaaat aagaatgccg
     3481 ctgttaaaac aatacttgat cctgctgcta tgttaaaact ataaccaatg aacaaaccta
     3541 gcaatgatga gcacgctccg actgtcgccg aaataaatat catagttttt aaactgtttg
     3601 catataaata tgctgtagca gctggtgtaa ttaacattgc aacaattaat atagttccca
     3661 cactttgcat agctgttact gctactaaag ttaatagaat cattaataga taatgataaa
     3721 aactgacatt catccccatt gcttttgcca ttaatggatc aaaagaagtg attaataatt
     3781 ctttaaaaaa tagaacaact gtaaccaaca ctaaaacaga aacgccaatt gtaatccact
     3841 tatcaatatc ttgtacagct aaaatatttc caaataagat atgaaataaa tccgttgagc
     3901 tatttgcaac tcctataagg ataactccta atgctaaaaa agaactgaaa gtaataccaa
     3961 ttgctgtatc tccttttatt acactattgc tttttataaa agtaataatg atagaagcta
     4021 atagaccaaa tacaatcgca ccaataaaaa aattaatacc taaaatataa gaaattgcta
     4081 ctcctggcaa aacagcatga gaaatagcat cacccataag agacattcct cttaaaataa
     4141 taaaacaacc tactgttcca gaaacaatcc ctataactac agatgtaatt agagcattct
     4201 gtaaaaagtg aaagtcatac agaccattaa taaaattttg aagcatatta ttccccttgt
     4261 atattagagt tagggtttta accttccggt tttgattgga taaccctatg aaccatcgtt
     4321 attttaattt ggtattaact catttctttt gagataatat atttattgga ttgagaggct
     4381 aagccgttcc cccctctcca ataaatatag tatctccata ggcttttttt aaattctctt
     4441 cattaaatac agattctaca cttccgtgag caattagatt tcgattcaaa agaataatat
     4501 tatcaaaata cttctttacc ttgcttaaat catgatgcac aattagcact gtttttccct
     4561 gatgtttcaa ttcttttaac gtatccataa ttattcgttc actaactaag tctatcccta
     4621 caaatggttc atctaaaaat atgaaatccg cattttgagc aagacatctt gctaaaagta
     4681 cacgttgaaa ttgtcctcca gataattctc ctatttgatg attagaatat tttcccatat
     4741 cgaccttttc caaagccttt gaaactcttt gccattctgc atttttaatt ctttgaaaaa
     4801 ctttcattcc ggcatatgtt cccatagata cacattcttt tacttttatt ggaaaagtaa
     4861 aatcaatatc agctttttgc tctacatatg caatcttcac cagtttttta ttgatatctt
     4921 cattattaat ggagacctct ccctgatgag ggataatatt taacattgct ttaagcaatg
     4981 tagattttcc agcaccattt ggaccaataa tacctgttaa agcaccttct tgtattttta
     5041 aagaaatctc gttcaatgct aaaaaatcgt tataacaaac attaagcttt ttcacttcta
     5101 acataacttt ctccttctaa atg
//
LOCUS       DAYYAR010000159         5115 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000159 DAYYAR010000000
VERSION     DAYYAR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.162"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..1269
                     /locus_tag="ACHAT0_002582"
     CDS             34..1269
                     /locus_tag="ACHAT0_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8452478.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKPEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFAVLKRKINS
                     YQNPESHKTKLYCGAESFY"
     gene            1242..1322
                     /locus_tag="ACHAT0_002583"
     CDS             1242..1322
                     /locus_tag="ACHAT0_002583"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452479.1"
                     /translation="MLWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1448..2527)
                     /locus_tag="ACHAT0_002584"
     CDS             complement(1448..2527)
                     /locus_tag="ACHAT0_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8452480.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2630..3070)
                     /locus_tag="ACHAT0_002585"
     CDS             complement(2630..3070)
                     /locus_tag="ACHAT0_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8452481.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3127..3537)
                     /locus_tag="ACHAT0_002586"
     CDS             complement(3127..3537)
                     /locus_tag="ACHAT0_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8452482.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3717..4151)
                     /locus_tag="ACHAT0_002587"
     CDS             complement(3717..4151)
                     /locus_tag="ACHAT0_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8452483.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4251..5099)
                     /locus_tag="ACHAT0_002588"
     CDS             complement(4251..5099)
                     /locus_tag="ACHAT0_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8452484.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1878 a          990 c          870 g         1377 t
ORIGIN      
        1 ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat cttaatatca
       61 acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac agaggagata
      121 gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac ctttcaggat
      181 gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac agcgaccatc
      241 aaagcaaaaa ttgactattc aggagactta gcacctatac tacaatcagc tgttacattt
      301 gagaatgttg atcccgcttt gtcgattggc tatgacgaga acactttgac tatccaacac
      361 aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa accagcgctt
      421 tttactgtca aggtatcaga tggactaccg aacgatcatc atcatttaac gccgtatagt
      481 cagcggcaga caattgagca agattcagct atcgactcag gaggagatct tgttgaagaa
      541 ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga aaatccagac
      601 ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac tcaaccagag
      661 ccagacaatg aatcaaaacc tgaaataacc cctggagaaa aaccagatgt agacccagaa
      721 gaaaaaccag atgtgactcc agaaccagat acggattctg gaaaccaaac agtaccggaa
      781 acaaatccag acacagataa tgaaactgaa aatccagaaa aaccagaggt agccccagaa
      841 gaaaaaccag atgtaactcc agaaccagat acggattctg gaaaccaaac agtaccggaa
      901 acaaatccag acacagataa tgaaactgaa aatccagaaa aaccagaggt agatccagaa
      961 gaaaaaccag atgtgactcc agaaccagat acggacgcaa gagaccaagg aataccagaa
     1021 aaaataaaca aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa atcaaatcta
     1081 gcaattctaa aaataaatga agagcagctg aataaaaaat cacgaatatt tgatagcgcc
     1141 caatcagctg aaaccttaaa aagtagcaag gatacaacct ttgctgtcct gaaacgaaaa
     1201 ataaacagct accaaaatcc ggagagtcac aaaacaaagt tatactgtgg agcggaatca
     1261 ttctattaag tatagcaaca atgctttctg caaaaagatt taaacaaaac agatctctct
     1321 aacatttgaa taatgtctgt gaacgcattt ttcaaataaa aagaaatatc cagaaaatga
     1381 ccccaaaata ccagattgaa atctgatatt tggggtcgtt catactttat ctttcttcat
     1441 atttatttta agggatatct gtttcttttg gaagatggtt cgggatcttt ttcactttca
     1501 atacatctaa aaagaaggta aagatcggaa tgccgacaat caagccccaa acgccaaaga
     1561 accgttcgct aacaagtaag accacaaatg tataaaaaat cggcagttct gttttgctgg
     1621 acatcaactt cggattcagc acataagact caattagatg gacaatcaaa atcgtaatca
     1681 ggatataaac aacatctcgg atgcctcctt gggaataagc tatgaaagat agaggaatac
     1741 aagaaatgat aacacctgct actgggatca gacttaaaat gaaaatcatg atcgctaaac
     1801 tcagcagctg atgaaagcca aatacagcca aggcgattgt agtgagcaac gtatttaaaa
     1861 gagcgacaac aaattgagct tctaatacca aaccaaatgt attgacaaac ttatcggcaa
     1921 aatagtaaat atcttgaaag aaccaagaaa attcactttt taaaaacaat cttgagaata
     1981 caattgtttt tttcttttca atcataaaaa agaaacttaa gatgaatgac atagcaaacg
     2041 ccactgccat ttttcctata tcctgtaaat agccaagcgc aatcgaagcg ccattttgaa
     2101 gctgttcaaa aaaatcgttt cgctcaatat aagtatggat atattgtaaa acaggatctg
     2161 catcttttgg ctgatgctcg taaaaattaa taacggactg gatcatgtca aatgtttgat
     2221 gaataagcat cgggatatac tttgtgaatg ccagatagat aaggtaaacg atcagtgtat
     2281 aaatcatgat agctaaaaca cctgtaggga ttcggacaaa tcgctgaatg atttgaacaa
     2341 gatgaatcat caaataagta aagataaatg tgagcaaaat ggttgtaatc atacttctaa
     2401 ctaagaaaag aacaaaaacg acaaataata aaacagtgag acggcgtatt ttttcattcg
     2461 ccaaaaaacg ctgataaacg gtcaaatcga caacctccaa ataacaaaaa ataaacacgt
     2521 tattcattat atgatggaat atgaaaataa gaaagagcaa aaagaaaaac cgctaaatga
     2581 cgttatctta tgagttggtt ttcctcataa aatcgttcat ctagcagttt cagccctttt
     2641 tctggtcttg atgaaaatta tctagcattt ttcgggtcag atgttgcagt gtatccaatt
     2701 cttcctgttc aagtggaaga taagacttga tcttttgtgg aataccttct gcttgttttt
     2761 gcaacgcagc accttgagca gtcagcgtaa catttacgat tctttcatca cccgtttcac
     2821 gctgacgatt aatataattt ttttctgcta acttttttag taagggagtc agcgtaccag
     2881 aatccagaca caaatcattt cctagctctt tcactgaagt ctttttcttt tcccataaaa
     2941 ctaacatcac aagatactgg gtataggtaa gattcagcgg cttgaggagg ggagtgtagt
     3001 gtctaatgat ttctttagag gcggcatata aaggaaaaca tacttgatta actaaatata
     3061 gatcttccat ttttccacct ccgctgtttt actatagtgt atcatagcgg atttacacaa
     3121 aaaagtctat tcactaactg ctttcagagt aacttctata tttccagctg tagcttttga
     3181 atacggacac actttatgcg ctgcctctaa taattcttgc gtttcttcca gagaaagacc
     3241 ttcgatagat ccttcaatct ctacacctaa gacaaagctc gtttcacttt ctgaataaag
     3301 ggaaacttgt gcactgacag tagaagcccc agagatattt ttttgctgtt tgatcaattc
     3361 taatgcactg ttaaaacatg cactgaaacc agctgcaaac aattgttcag ggttggttgc
     3421 attttcgacg cgtttaccag gagctgcaat attcaattcg aatgtacgat cgggattatg
     3481 cacttctcca gagcgtccgc ctgtgttgat cattttcgta gaatacattt ttttcataaa
     3541 attaccacct ttaattatat tttatacaat tgaattttac acaatataat tgtggaaagc
     3601 aaagaaatgg attggcataa gataaataag agaaagcaca tgaaacagac gcgctttctc
     3661 gaatagcaaa catatacaaa aaatatcaga gacaaaaaat aatctcatca taaacattac
     3721 atatatttct caaaaagttg ttttctttct tcaaaaatta cttcacgatg gacctgatga
     3781 agcatccata aataaccaaa aggatctctg aacattgagt tagtcacacc aaagtccatc
     3841 atttctgtca tttcttgaat ctctacacag ccatttcgta atgctttatt atgtgtgtcc
     3901 tcgatatcag agacgatgat attgaaccag atcgtatttg gatgttcagc agtaggagca
     3961 gtcaaaccaa gagacgtgtt ttcatccagc atatggaatc tcgtaccata caaggtaaaa
     4021 atgacttcat tattgcctct agggtaatct gttgcctcaa tcagttgtat ttcatcaaag
     4081 attgtttgat acagttctag tgctttcaaa ctatcattta atacaaaatc gatttccgtt
     4141 cctttatgca taaagatctc ctctcctttt cagtcgaaca gttactagat tcttttatta
     4201 taccagctaa agaaaacgca ttcaaaaaaa tagcggattt acttaccaac tcattcgatt
     4261 ggatgataag taaatccgcg tcccattact tctgctacgc tgctgacaat gacaaaggca
     4321 cttggatcga tttcttctat caatggtttc attcgggaaa attcatgatc agtcacaacg
     4381 atcaataaaa cctcttttga ctttcgatta tacccgcctt ttgcctctag cagggtaagg
     4441 ccacgtccaa tctcagcact taagcgaagg cggatgtctt ccaaataatg ttcactcatg
     4501 accataacag atttctttcg attgatacct gtttcgatat aagtcatgac aattgaagtg
     4561 attgcaatag ataaaatagc ataaagaaac tcttctattc caaaaataaa cagattaaga
     4621 gaaacaacga atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg gaaatatttt
     4681 ttaaaaatga ggggagggat cgtcgtaccg ccgctagaag agttgttttt atataaaatc
     4741 gcaactccta aagcaaaaat cgcacttcca aaaatgacag acaacaaccg atctgaagta
     4801 atcattgttt ctggaactat tgcaatgatt aaaggaaaaa ccaagcttcc gaaaagtact
     4861 ttaaaaaagg gttttttacc taaaaataga agagctagta ccaacataac gatattcaac
     4921 actaaaataa ctatagctcg gtcaaagcct aaagcagctt ccagtaatat tcctagaccg
     4981 ctgacacctc cagcagcaat atgatgaggc cctaaaaaca tattgatagc gactcctaat
     5041 atgaagaggg caaaaataat cttaatgtat gtccatataa attgtctcgt ttctttcatt
     5101 tattcacttc cttaa
//
LOCUS       DAYYAR010000160         5107 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000160 DAYYAR010000000
VERSION     DAYYAR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.163"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..1893)
                     /locus_tag="ACHAT0_002589"
     CDS             complement(127..1893)
                     /locus_tag="ACHAT0_002589"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8452485.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1853..3607)
                     /locus_tag="ACHAT0_002590"
     CDS             complement(1853..3607)
                     /locus_tag="ACHAT0_002590"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8452486.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3717..4286)
                     /locus_tag="ACHAT0_002591"
     CDS             complement(3717..4286)
                     /locus_tag="ACHAT0_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8452487.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4304..>5107)
                     /locus_tag="ACHAT0_002592"
     CDS             complement(4304..>5107)
                     /locus_tag="ACHAT0_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8452488.1"
                     /translation="KELIDQVLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVP
                     YIEKVSADVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPT
                     KGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKM
                     KDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGA
                     VVIATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1696 a          957 c          683 g         1771 t
ORIGIN      
        1 aactaattca atcatactga ttctttcgtg tattcaaaat tagatgctgt ttaataaaat
       61 tattcttttt gaaaaagaat tataactata tcccaaaaat tgtaggttaa tttaaagcct
      121 atttcatcat cgtgattgag cttctttttc atagacgaat ttctcagata gtttagtttt
      181 ctttatgaga tttttgtata tttctgacct tcgatacaac tcactatgag taccttgatc
      241 aactattttt ccttgatcca tcactacaat tttatttacc ttttcaatag acttcaagcg
      301 atgcgatata attagtactg tcttatcttg tattaaacga tttaaacttt cctgaatacg
      361 tttttcattt tccgcatcta aactggcagt aatttcatct aaaattaaaa ttggcgcatt
      421 tttcaaaaaa gcccgtgcta tggataggcg ttgtcgctct cctccagaaa gtgaacttcc
      481 gttttctccg accaatgtat gataaccgtc aggtagtttt tcaataaagt cttcgcagtt
      541 agctaaacga gctgcttttt tcacttcctc atctgacgct tgactatttc caatacgtat
      601 attttccaaa atacttgtat taaacaaagt tacctcttga aaaacaatag cgattttgct
      661 aaacaaagaa gctggagata cacgtttaat atcataccca tcaattaaaa tacagccttc
      721 atcataatca tacagtctag acactaattt caagatacta gttttcccgg aaccactagc
      781 acctactaac gcagtcactt ctccttgttt agccgtaaaa gagatatgat ctaaaattgg
      841 ggtattgtta tcatatgaga aagagacatc tcgcaactca atgtcaaaag atttcagagg
      901 actatctgat ccctcttgga tactggtttc cttcatagct ctaattcgct gtattttagg
      961 agctaaataa aatatttcaa gcactgcttc tttcatagaa tcgaaggaat cctttatttt
     1021 tattgcggct agtaagtaac caacgacgta gagtatagtc acttcacccg ttaacagtaa
     1081 gtgaacacct actagaatga ctactgctaa actaacatat gagaaaatag aggataaggc
     1141 catgactgaa aaagtactta attctacctt taaatgaatc ctttcactct cttccatttt
     1201 tttgaaaagt ctgtcttgaa cttttttaga tagattaaag ctattaatct cttgctgcaa
     1261 ttctatagtt tcttgaaatt cttccgagtt ttctctcaaa gtatcgtaat atttagtatt
     1321 ggctttcgtt tgggatttct ttgataacaa gattaagaca aaactaaata acgttggcaa
     1381 aataacagct aatcccattt tgacattacc aaccaaaagc atcactgaaa taaaagggaa
     1441 aaacagagcc ataccgccgg ctttaggtat tgcatgactc atcgcatgct caataccttc
     1501 aacgtcagac atgatagttt gagataaatc tgataaatta tgttttgaaa aataggataa
     1561 tggtaaattt gacaaatcgt ctgcaatttg cattcttaaa tgcgcacttt ctttataagt
     1621 actattatat aagttttcat actccctatc taacaaccaa tacatgaaaa gcaaagtaac
     1681 gaacgatact gtaaaataaa gccaaggcgg ctttacattt tctaaaacta attcgtctat
     1741 cagcatcatt ataatgaatg ctggtaccat attaatgcag tatacaaaaa agctagaaat
     1801 agatgcttta accaaatctt ctgcgccctg attagttaaa gcgaatttgt ttttataaaa
     1861 acttttcatt gtcaagcctc caatcatttg cattctcata attagtgacc aattgtttat
     1921 acacttgatg tttagccatt aattcattat gatttccacg ttctactatt ttcccttctg
     1981 acaagacaag aatttcatct acagatttaa tgctggataa tcgatgagct atcataataa
     2041 ctgttttttc tttcatcaga tgttgaaaag ccttttgtag ttcatactca ttatctggat
     2101 caatagatgc actggcttca tccataataa caatctttgc atcttttaaa attgctctgg
     2161 caatagctat acgttgtttt tctcctcctg acagataaac tcctttacta ccaattaagg
     2221 tatgctctct ttcgggaaat ttttctaaaa tcgattcgca accggctaat cttagcgcgt
     2281 caaatacttt acttgctggc gcttgaggat ttgctataga aacgttatcg taaatactct
     2341 gattgaatag tttcgtatct tgaaagacaa aagcaattgt gttaataatt gtgtcttttg
     2401 aatagtgatt taaaggctta ccaccaattt taatctgtcc cttatccaca ttgtagaaac
     2461 ctgaaattag tttagctatc gtactctttc cgcttcctga ttttcctact aatgcataaa
     2521 ttcttttttg ttctaaaact aagttgaaat tttctaaaac acactcttct ttgtatgaaa
     2581 agctgacatt ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc ccatatttta
     2641 gttggtcttg cttcatttct aaataaaggg agtctagtgt ttcgactgca tatcttgctt
     2701 caaacaagtg catacttaca tacattattc tcatgaaaga aacaaaaata attccactta
     2761 aaaaaaacaa catcaacaag tcaacagcta atatcttggg agtggtaaga gaagttataa
     2821 aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa
     2881 caaaaggtgt tttcccactc aaagaatact tatatgcaaa cttagcataa tcgttaattg
     2941 ctttattcaa ggctttaaat gatgttactt tagcaccaaa tatctttata acttgaattc
     3001 ctcggatata ttctaccgtt tcagagctta acacatctaa cgattcttga tatttttgaa
     3061 taaaatttgt attctctgtc attttcttta acaagaatac accaattact aacatggcga
     3121 ttagaaccaa tccaatttta aaattaatga aaaagcctaa ggcaactgat aaaacaggtg
     3181 ttactatggc ttgagcacta tctggaatca agtgagcaat tgccatatgt gttttagccg
     3241 cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac
     3301 ttgcttctgt taaaccgcta attccctttt ttctcaaatt agtctctaag cgaaacccaa
     3361 ggatatgaga aaataagcca gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa
     3421 cagtttgaac cgcataactc ttagctaaat attcatctcc tacaataatt agagacttaa
     3481 gaaatttgta aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata
     3541 aagtggctaa ccatccgtag tatttcattt tagggacata actaaataat tttttgtata
     3601 ctttcatttt ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg
     3661 ctttccgtaa tagcgtaaat tagagatgta caaacagata gaaggtaaac tacgttctat
     3721 tttttgtatt tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct
     3781 acaactaaca ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt
     3841 gttgcagcag attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat
     3901 ataggaatcc actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga
     3961 ggaattgctc gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta
     4021 atcgaactga taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa
     4081 acaattgtgt aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat
     4141 atgattccag ataataatcc aaaaatccct ggggatatgc tgtgaccaaa cgccccacca
     4201 atactactaa ccattattga aaaaaagtaa attactaggg aaataactgt agctaaacca
     4261 ataaaaatat aatcttttgt tttcatatat attctcctca attctatttc gttatgtcat
     4321 taaaaatctg ttctatcaaa gttgaatctt ctactctaag ttttatatct ttttttattc
     4381 caccattttt taaataaata attctattgc atgttttata aagaaattcc ggatcatgag
     4441 tggctataac tactgcaccc ttttctttta agtctttaat aaggtcagca atcctttcca
     4501 tattaatata atccaaccca cttgtaggtt catcaaatag aaataaatta gtttttgaca
     4561 atgaagctat agaaaccaat aatctttgtt tttctccacc ggataaatca aacggtaatt
     4621 gatctttcat tttataaagt tccatttttt ttaaaatttt ttcttgctga tctttactaa
     4681 ctatttcatt ccccagggct aactcgctcc tcactgattc tgtgaaaaat tggtaatcca
     4741 tttcttgcat tactaaaaac ggcgatgaca aatttttttt attttcgatt atctttcctt
     4801 tggtagggcg aataatcttc atgagtattc ttagtaaggt tgttttcccc acaccgtttt
     4861 tccctaccaa accaataaca tctccaactt tgacatcaaa atttatgtct tttagaatat
     4921 tttcataagt aatttcttca acttttaata catctgcact taccttttcg atatagggaa
     4981 cttcaatcga actattaaaa atgtcaaatt tccttaatgc gttattgttt tcagttttta
     5041 acaactgaat agttgtagtt tttacagttc tatttttaac aaatagaact tgatcaatta
     5101 attcttt
//
LOCUS       DAYYAR010000161         5087 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000161 DAYYAR010000000
VERSION     DAYYAR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.164"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHAT0_002593"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACHAT0_002593"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFM8452489.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHAT0_002594"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACHAT0_002594"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFM8452490.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHAT0_002595"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACHAT0_002595"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFM8452491.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACHAT0_002596"
     CDS             complement(4028..5044)
                     /locus_tag="ACHAT0_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8452492.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
BASE COUNT         1337 a         1270 c          909 g         1571 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaa
//
LOCUS       DAYYAR010000162         5013 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000162 DAYYAR010000000
VERSION     DAYYAR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.165"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..165)
                     /locus_tag="ACHAT0_002597"
     CDS             complement(<1..165)
                     /locus_tag="ACHAT0_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8452493.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
     gene            complement(367..762)
                     /gene="panD"
                     /locus_tag="ACHAT0_002598"
     CDS             complement(367..762)
                     /gene="panD"
                     /locus_tag="ACHAT0_002598"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFM8452494.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(772..1620)
                     /gene="panC"
                     /locus_tag="ACHAT0_002599"
     CDS             complement(772..1620)
                     /gene="panC"
                     /locus_tag="ACHAT0_002599"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFM8452495.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1635..2462)
                     /gene="panB"
                     /locus_tag="ACHAT0_002600"
     CDS             complement(1635..2462)
                     /gene="panB"
                     /locus_tag="ACHAT0_002600"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFM8452496.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2474..3334)
                     /locus_tag="ACHAT0_002601"
     CDS             complement(2474..3334)
                     /locus_tag="ACHAT0_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFM8452497.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3572..4741
                     /locus_tag="ACHAT0_002602"
     CDS             3572..4741
                     /locus_tag="ACHAT0_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8452498.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT         1610 a          942 c          631 g         1830 t
ORIGIN      
        1 tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag aaggcgtaag
       61 ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat cgacgatttg
      121 ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt gacatgaaaa atcctccagt
      181 gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta gccgattcac
      241 ataaaatgga ctttgtctac agtctgtatc ttctcttttt gtgtctactt tattgacatc
      301 tatccaatta gtaatcaata aatatttcaa taatagtcta gctatatata aataacatta
      361 caaaacctaa agaattatat ctgataacct tccatgtttt tcatatgatg ttactctttc
      421 aactttatta ttatcatcga caaaaacaac cttaggtcta tgatccttaa cctcattttc
      481 atttaattca caatatgcca ttaaaattat tttatctcca gttgatacac atctagctgc
      541 agctccattc aagcaaatca tccctgttcc tctctctcca gctattgtat aagtttcaaa
      601 tctatttcca ttattaacat caactatttg tactttttca tattcatata ttcctgctgc
      661 ttctaataaa ttcatatcaa cagtaatact tcctacataa tctaactcag cttgttcaac
      721 tgtagctcta tgtatttttc cttttaacat tgtaactttc attatagccc cctattgtaa
      781 ttcaaatgta aaattatcta ttaatcttgt ttttcctata aatactgcta ttgcaactaa
      841 aactgatttt tcaatataat ttactctttg taatgataaa gaatcaacaa cttctacata
      901 atcaatcttt gccaaattat aagtatttat atttttactt attatctcaa ttatttttaa
      961 gctgtctctc tctccattat ttaaagcttc cttagctaaa gttaaagatt tatttaaaat
     1021 agtagctgaa gatctttcct ctaaacttaa gtatgtgttt ctagaacttt tagctagtcc
     1081 atcctcttcc cttataattg gacaccctac aacgtctata tcaatattaa ggtccctaac
     1141 cattctttta attacagcta actgttgtgc atccttttct ccaaaataag ctctatctgc
     1201 tggtattatg ttaaataatt tacttacaac agtgcaaacc cctctaaaat gtattggcct
     1261 actagctcca cataaacctt ctgttaatcc agttatatta acaaaagtac ttgcattgtc
     1321 acaatacatc tcttcaactt caggattaaa tattgctgtt gctctacctc ttgtgcatac
     1381 ttctatatct ctttctaagt ctcttggata actacctaaa tcttcatttt tcccaaactg
     1441 agttggattt acaaagatac ttacaacaac tttatcattt tcttttgatg cttttctaat
     1501 taaactttca tgaccttcat gtaaatatcc catagtagga actaatccta cctttaagcc
     1561 ttgttttttc cactctttta cttgacttct aacatcattc actgttttaa atataaccat
     1621 atttcttgac ccttttaata taatttttct aaaacatcat cacttatttt aaacccatgt
     1681 tctggacctg gaaaaactcc atcttttact tcctctatat attttgtaaa tgctttattc
     1741 atctcttctg ataaattagc atatttcttc acaaatttag gggtaaaatc actatacatc
     1801 cccaacatat cttgatatac aagaatttgt ccatcacacc ctgcacctgc tccaatacca
     1861 attgtaggta ttgatatctc tttagaaatt aactctgcaa gcttagttgg aacacattct
     1921 aacactacag cgaatgctcc tgctttttct acagctttag cagctcttat taattcttta
     1981 gcagcttcct tgtcctttcc ttgtacctta aatcctccaa atgcattaac agattgaggt
     2041 gttaaaccta tatgcgccat aacaggtata gaagccttta ctatagcctc tattttatcg
     2101 catacttcta tccctccttc taatttaaca acctgagctc gtccttcttt tattaatctt
     2161 cctgcattaa ctactgaatc atatactgaa gtttgataag acataaatgg catatctgca
     2221 acaactaaag tgttcttagc tcctcttgta actgctctag tatgatgaat catgtcttcc
     2281 attgttactg aaagtgtatc ttcatgtcct agacatacca taccaagaga atctcctact
     2341 aatattccat ttatcccagc ttcatcaatt atttttgctg ttgaatagtc ataagctgta
     2401 agcattgtta atttttcatt tcttaattta gattctttaa atgtaactgc tgtatttttc
     2461 atctatttct ctcctaactc tctcctaaat acttttctaa ttcactataa tctctattaa
     2521 tattttttaa catagctatc tctaatacat tttttgataa tagcatatat aaagctctgt
     2581 cttcatctga taaaacttca cagtgacctt taattgttga aatatcacaa cgttcaactg
     2641 gccctgtcaa actttctata attccttttt cttttatatt tctaatatta aattcaatta
     2701 atggatataa agcttcaatt gccatttttt cagtaaatcc acattgaatt agatatgtaa
     2761 cgcttgtatt aataagtcct agcacaaggt tactagatac tactgatgcg gcatgataaa
     2821 gagatttatt ctccttagat acaattacag tactattacc taactttttg aagaaatcac
     2881 ataaaaactt tgcatatttt tcatcgcctt caattgttat aaaagatttt tttaattttt
     2941 tgtatgaatt atatttatct gatattgaaa acataggatg gacagaatag ccataagcac
     3001 catagtcctg tatatttgaa aagatatctg aagatattgc accactgcaa tggcagatta
     3061 atttgttttt gatttgataa tttttaattt cttgccatac ctcctgaatt tgaccatcag
     3121 gagtcgtaat gaaaatagta tcattttcat ctactaattc tcttaagttt aaatattgtt
     3181 tactagatgt aaaattcgaa gctgctaatg acgagtcttc gcttttacta taaaatcctg
     3241 ttacttgtat tttatgttcc tggaaatact tcccgaatga acaacctacc ttgccggctc
     3301 caataaaacc aacgttcata attatcacct ccataaagaa gtgagactac aagtcccatt
     3361 cttacgatat gagcttaaaa ataaaaatta aatcaaatat acagtatgtt tttttcaaat
     3421 atcatccata gttaatttat cataatatcc catattttaa aataaaagat agcgtaaaat
     3481 attttgtgta aatagaaatt tagggtagaa aaaaaagtcc attctgtaaa attaaagtta
     3541 ccacacagaa attttatagg aggactttct catgactaaa tttactatag aaattatgca
     3601 aacactactc aataaagggg atttagatga attatttcag gatcatttag agcgagcgat
     3661 taattcactt ctacaagcag agctaactgc ttttttagat tacgaaaaat atgatcgaaa
     3721 tggattcaat tcaggaaatt cccgtaacgg gaattattca cgtacattta aaacagaata
     3781 tggcgagttg aatttaacta ttcctagaga tcggaatggc gaattttctc aacaaacact
     3841 accagcatat aagcgaacca atgattcatt agaaacgacc attatccaat tatttcaaaa
     3901 aggcattact atggctgaaa tctccaaatt aattgaaaaa atgtatggtc accactatac
     3961 gccacaaaca atctccaaca tgagtaaatt ggtggctgaa gatgttttag cttttaaaga
     4021 aagaacgtta gaagccaatt attccgttat atttatggat gcgacgcata tccctgtgaa
     4081 gcgacagact gtttcgaagg aagcagtgta tattacgatt ggtattcgtt tggatggaac
     4141 caaagaagtt cttgggttta ctattgcccc aactgagtct gcttatattt ggaaagaagt
     4201 tcttcaagat cttagaaaac gtgggttaga agaagtttta ttagtagtga cagacggatt
     4261 aagctgtatt gaagaaagta tccatagtgt gtatccgaat gcccaatttc aacaatgttg
     4321 tgtgcatgta tctagaaata tcgctcataa agttcgtgtt cgaggtcgaa aagaaatttg
     4381 tgaggatttc aaattggttt accaagcgaa ttcaaaagaa gaggcattgg atcacatcga
     4441 ctttatgact aggaaatgga aaaagcagta tccaagagtc gtcaatttac tcttgaatcc
     4501 tgccctatta accttttata atttccctca cgccatcaga cgaacaattt attcgacgaa
     4561 cctgattgaa ggctttaata agcagctaaa acgatatact cgaagaaaag aacaattccc
     4621 taatgaagaa tctctagaga gattcttcat ttctcaattt aatcaatata accaaaaatt
     4681 tttaggtaga attcacaagg gatttaaaga aattcaggat acattagagt cgatgattta
     4741 actgtaatta acagaatgga ttttccattt acacataatt cttgacgcta ccataatcta
     4801 aataaccatc atattcatct actacttata taattgagtt ttattaaact ttttaagttt
     4861 ctattctatc aaatatttaa acaatctctg catccgaaaa gactacctcg tatagtttta
     4921 ctaacaatat ttttattagc gtgttcaaat cctgatttct tgtatcaatt attttctgtt
     4981 cactttcaat aagaaaaaag tctttacttc cat
//
LOCUS       DAYYAR010000163         5003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000163 DAYYAR010000000
VERSION     DAYYAR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.166"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1292
                     /locus_tag="ACHAT0_002603"
     CDS             72..1292
                     /locus_tag="ACHAT0_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8452499.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1289..4009
                     /locus_tag="ACHAT0_002604"
     CDS             1289..4009
                     /locus_tag="ACHAT0_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8452500.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3993..4937
                     /locus_tag="ACHAT0_002605"
     CDS             3993..4937
                     /locus_tag="ACHAT0_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8452501.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         1860 a          819 c         1005 g         1319 t
ORIGIN      
        1 aaattttcgg ttattgatga aaaaaaacat tgaaaatggt atgataaaag aaaaaaagga
       61 ggaaattttt aatggtgaaa tttatccatg cggcagattt acatatggat cggtcatttg
      121 aaggactgac aactcttgat aaaactgtac aggaaaaact attgaagacg aatctgaccg
      181 ttttatcaaa tatcattgaa caggcaataa taaataacgt tgattttgtt ttacttgcag
      241 gagataattt tcatcaaaat cgtccttcat taaaaataca gaaacatttt tcagaacaga
      301 tgaaacggtt agaaaagaac catatacctg tatttataat attcggaaat catgatttct
      361 atcaaaaaga gcggtattgg ttctcctttc ctaaaaatgt ccatttattt acatccgaaa
      421 cggtagagac aaaaaaaata acgataaaaa gtggagaaac tgtctctctc agtggattta
      481 gttatcgtca accgtggatt caaaaagaca aagtgatgga atttccttct agagaactca
      541 cagattacca tattgggttg taccacgggg aacctggcgt ctctcaaaaa ggaaactatg
      601 ctcctttcca accctcaaag atgcaagaaa agggatacga ttattgggct ttagggcata
      661 tccatgtgcc aacggtctta aatgaaaagc aaacaattgt ttatccaggt gcgcctcaag
      721 gtcatactaa aaaggaacaa gcttctacat ctattctact agttgaatta agtaaaggaa
      781 gttgccaaat acatccgatt aaagtagcag aggtctattg gaagacaaag gaaatatcat
      841 tgaggaaagc aagaacgaca aaagaggtca ttgttcacat tcgtcagcag ctttcaaaag
      901 tagaggatgg atttagttta attgagataa aattaaaaga ttatgaccat ctcaatacag
      961 atgttttgga acggatacaa tcaggagagc tattagacta tttgttagag gaattctcag
     1021 ataggataaa tgatttcttt atttggcgta tttctctcat agagaatact ttttatacga
     1081 agaccccact tgctgcttct gctaaattga tggaacagtt atttcaatac tatcagacac
     1141 ctcaagattt ccaacaaatt ttaaatgaag tatatagtca tcaagaagcg gctagaatat
     1201 tgagcgaact gccggaatat caagaagaaa cacttgaaaa agccaagcag ctgcttaatc
     1261 aagatttttt atttgaggag gatcaggaat gaagatttta aggatcgaga ttgctgcttt
     1321 tggaaaatgg cgtcaaaaaa gttttgactt ttattcaggc aatcagctga tctatggggg
     1381 aaacgaagca ggaaaatcaa cgatttatca atttattcaa gcaatattat ttggtttccc
     1441 ttcaaaaggc cgtaaaaaaa aggattatac accaaaagac ggctcagcct atggcggcaa
     1501 aatatggctt aaacatcctg tatatggtga atttgctgtc gagcgctaca agcagcaaaa
     1561 tcgcggaaaa tcgaaggtgt ggctggggga tcaagtggga agtgatgagt tactagaaaa
     1621 actgatcgca cctttgacga aagagttatt ccagcaggta tttactttcc agcaagagca
     1681 gttgatggaa ctagatcatt tgagagaaaa agagctacat gatgcgctga tttctttagg
     1741 catcacgggc agttctcaag tattccaaaa gcggcaagaa tattatcaga aagcacaaca
     1801 attattcaag aaaaaaggcc aaaaattact aatcaatcaa aaattaaatg aatggaaaga
     1861 gctgcaagaa aaaattcatc aaaaacaaga acaggaagct tattttggca agttgatcca
     1921 acaagaacag gaatacgagt cgcaacagca gcagttattg gcacaattaa aagaggcgaa
     1981 caaacaattt tcattagcca gacagcaaga acttaatttt tctttatatg aagaatggca
     2041 atctctcaaa aaaaacagca ctacaggtct tcctgatgag caacttcttt tagatctgga
     2101 gtctttctcc aaacggtatc agcaaacaaa tgagcgtttg gaagaattag aagcaacact
     2161 ggaaaagcat agcgggatgg atcagcagtc agccagatat tatttttatt tagagcagga
     2221 gcaggaacta aaagatctcc agcaacttca atttacagcg gaacaattaa acgaagaatg
     2281 gcaaagatta caaattgaga ctagagaaac aaattcagaa ttgaagattt tggaacaaag
     2341 gtggcattgg cagaaaaatc cgttaccgga agaattttta tatgaagaag aatggaagca
     2401 tcttctggct gaagaaagaa ggattgctga tctagaaagc cagatttctg tgcgtctcca
     2461 aatagctaaa gaacagcaag agatgattga agcagaagtg aatgacttgg aagagaccta
     2521 tccagcatta ttacaaaatc agtcagagat agcaccagtc tcttcatcta aaccgtatat
     2581 gctgatggct ggcggagcaa taatcattgc aggtatattt gtaccaatgc ccttgaaagt
     2641 gatcctttta ctaattggtg caggcatgat tggctatttc ttctacaaaa ggcaaccaaa
     2701 aattaaaact aaaaatgatt tctctgagat aaaaggaatg tggcaagaaa aactaggaca
     2761 acttgatcta gccttagctc aattagctga agcagaagaa aaacaaaaga gaactggaga
     2821 agaaaaacag attcttcagc aagaagtata tcgttttgcg gatcatcggc acttaggaaa
     2881 gatggatacg ctcaaactca tgaaagaata tggacaggaa gtcatagatt atcagaaatt
     2941 atatggcgta tttgagcgga aacataaaag agaacaggaa atacaagaaa aactacgaca
     3001 aatcgaccaa aaatttgagt ttgttaaaga atggttaccg atacaagata agacaatttc
     3061 agaaaaaatg gtattgcttt cccgttttat acaagagatg gaacagatga aatttgccag
     3121 aagctatcag caaaatactt tattgaagca agaaatcaat caattgaaaa agaaacaaaa
     3181 agtatggata caagaataca gcgaacaatt gactcaagca ggcttatctt atccttcgga
     3241 agtacctgtc tttttacaaa aagccaaaca agccgaacag cagatgaaaa gatacaatga
     3301 attatcggaa atgcttactc cactatttcc tgaagagctg acaaaagaga aattatctgt
     3361 aagactgcgc caactgaaag aaaagcaatc ccaactccag caagaagaaa aagaagcaag
     3421 tgaaaatcgg caacgactcc aacttcaagt agaagagttg caaagagacg gcacattgga
     3481 cgaactttat caacaagcta gccggcaaaa agcaaaatta caggaattac tggaggaatg
     3541 gggagcactt gaaatcgccg ggacattctt aggtgatctt tcaacagaga tgagtgaaag
     3601 acaattaccc cagttgttaa gagcagcagg tgattattta ggtattttaa caaaaggaaa
     3661 ttatcagaag atattactga aaaatgatac actgacttta acagacggat atacagaatt
     3721 tcccatctat gagttatcta caggtacgaa agatcagctg attatggcga tgagatttgc
     3781 ttacctctca ttagaagcta accgttcgat atgcccgatc atcattgatg atggctggct
     3841 tcattatgat catcagcgaa agtatcaatt agctgaactt ttagctaaat ttggagaaaa
     3901 gtatcaaata atttgttttt cttctgataa agaaatggta agctattacc atgagatgaa
     3961 ccaaatcgta aaacaattag aaggagcaaa taatgaaaaa aattcgtgat ttagtaatcg
     4021 atgagcagtt tgaacaattt gtcctgatca aaagtgcaga tgtaagaatc gctaaaaacg
     4081 gaaaaaaatt tattgctttt actttccaag atacttcagg tactatcgat ggtaaatact
     4141 gggatgcttc ggaggaggaa atcaataaat ttacagccgg cacagtcgtt tttctaaacg
     4201 gtaaaagaga ggtttatcaa ggaaaccctc aagtaaagat tcttcatatg cgcttaacaa
     4261 gggtagatga gccaggagag cctagtttat acatggaaag agcccctttg aagaaagaag
     4321 aaatggaaga agaaatcaat caattgttgt ttgagatcac caatgcgcat tggaatcgta
     4381 ttgttcgcta tctacttaat aaatatcaaa aacaattttt tgagtttccg gcagcaaaaa
     4441 gaaaccatca tgcatttgcc agtggattag cttatcatac tttgacaatg cttcacttag
     4501 ggaaatcaat cgccgatgaa tatcctgagc tgaatcgagc attgttgtat gctggtatca
     4561 ttttgcatga tttaggaaag gtgattgaac tttccggtcc tatgtcgaca gaatatacgc
     4621 ttgcaggaaa tttgatcggt catctggtat tgattgacga agaaataact aaagcctgtc
     4681 ttgcgttgaa aatcagcgaa gctgacgaag atgttctcgt attacgtcat atggttttag
     4741 cacaccatgg cttattagaa tatggctcac ctgtacgtcc aagaataatg gaagcagaaa
     4801 tattacatca aatcgacaat atcgatgctt cgatgcagat gatgttgact tcgattcgtc
     4861 aaacagaacc aggaaaatac accgatcgga ttttcggtat ggacaaccga agtttctatg
     4921 taccaaaaga tttatagcaa aaatttattt ttttcattca aaacaaaaaa agcgagacat
     4981 aagtcttcgc tttttttgtt ttc
//
LOCUS       DAYYAR010000164         4956 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000164 DAYYAR010000000
VERSION     DAYYAR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.167"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..828)
                     /locus_tag="ACHAT0_002606"
     CDS             complement(55..828)
                     /locus_tag="ACHAT0_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8452502.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            972..1322
                     /locus_tag="ACHAT0_002607"
     CDS             972..1322
                     /locus_tag="ACHAT0_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8452503.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1303..2019
                     /locus_tag="ACHAT0_002608"
     CDS             1303..2019
                     /locus_tag="ACHAT0_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8452504.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2019..3467
                     /locus_tag="ACHAT0_002609"
     CDS             2019..3467
                     /locus_tag="ACHAT0_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8452505.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3537..4046)
                     /locus_tag="ACHAT0_002610"
     CDS             complement(3537..4046)
                     /locus_tag="ACHAT0_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452506.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4036..4761)
                     /locus_tag="ACHAT0_002611"
     CDS             complement(4036..4761)
                     /locus_tag="ACHAT0_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452507.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1703 a          843 c          998 g         1412 t
ORIGIN      
        1 ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat
       61 cagatcaaaa tacttcaatg cgtggacaat tccgccttct gtatttttct ttgtgataaa
      121 ggtagaaagt tcttttaatc cgtcataggc gttgtccata gcgattttgt tgtcgcaagc
      181 ttccaacaag gcaaaatcat tcggaccatc gccaaatcca aaagtaggaa ctttttccag
      241 ctgaagttct ttttgcagac gtttgacacc tgcccctttt gagattcctt tcttcactgt
      301 atcgatcgag taaggaccat tccgataaaa agttagctca ggaaaatgtt cgtgatagta
      361 ttcgtcacct gattgagata agatcagcaa catatttact gtgttttctt taaagcctaa
      421 aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct
      481 atgaccggta caccagattt tctgatcgtt atagtaagaa agttcatgac cgttcaactg
      541 tacatgatca tacatttttt gacattcttc tgaagaaaat gtttgagaat agatttcttc
      601 gccgtctact cgaatgaaag aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc
      661 catgatagag tggatttctg cttctgtacg tcctgtagcg atgacgggga gaacattgtt
      721 ttcttttaat gcgttcatag cttttgccac ttcgggtgtg atttttgatt tcgcatctag
      781 taatgttcca tcaagatcaa agaaagtgat tgcacgataa tttgacacaa ataaaactcc
      841 ttttttattg gaataagttt atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa
      901 gaattcctga acatattggt tcagtcaaca atttagagta tactataaga agagaattta
      961 ggagggaaag aatgattttt ttcagtattg gtatcttatt cttaataatt ggcagtcttt
     1021 ttcggattct gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg cctcttgccg
     1081 caaaaacaga cgctcattgg cggctggctc aaaagacaag cggaaactgg ttttttctta
     1141 tgggcttact gatggcattg atcggctatt atttaaagac atctgggcat acgaattatt
     1201 ttctgattga aatgcttctg ctagtatttc caattatgcc tatttttatt atgacagaaa
     1261 agaaattaca gaaatatgac ctggaaactg gagggaacga taatgaatat tttaatgatt
     1321 gaagataatc aatctgtttc tgaaatgatg cagatgtttt tcttaaatga aggttgggaa
     1381 gcaactttca agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat
     1441 tgggatatga ttacactaga cctgaatttg cccacactgg acggtatgct agtaggaaga
     1501 gagatccgca aagtttcaag tactgtgccg attatcatgc tgacagctag agattctgaa
     1561 agcgatcagg tgatcggctt agagatgggg gcagatgatt atgtcacaaa acctttcagt
     1621 cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca
     1681 agagaagaag cagcagtttc agatgatcat tttgatatcg agactagtca tttcaagatg
     1741 aatacgaaaa cacgagaagc gtatctggac aaccggccaa ttgaaggact gacgcctaaa
     1801 gagttcgatc tattgtttac tttagcaaaa aaaccacgtc aagtattctc tcgggagcag
     1861 ttgcttgaat tagtctggga ttatcagtac tttggtgatg aacggacagt ggacgcccat
     1921 atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca aactgtttgg
     1981 ggagtcggct acaaattcga tgattctggt gttgcctaat gcgttatttg tatcagcaac
     2041 tcttagcttt ttgcggaatg gtcgcgttga tcttattgat cgttggagtt tcttttacac
     2101 aactgacaaa acaaacgatt gaagaaaaca attaccagca actgtttgga tatgctgaat
     2161 cagttgaaaa gacaacgcaa acctatgccg atacgctccc gcagtttagt caagatcagg
     2221 cgtttcaaaa tgcattgctg ctgacagagc aagtactgac agaacaaaat gtgaacttta
     2281 tatttatcga taaaaacgaa cgtgtcattt atccgactaa tacagcttta ctgaatttta
     2341 ccttaacagc tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga
     2401 ataaaaatat tttaggacaa gcacaggcga cctcttacgc acttgtgcca tttaatttga
     2461 atcatgaatt ctatggagca cttgtagtta gtcaacctgc gcgaaatatc gataacagcg
     2521 ttcgtccaat tacactgaac ctgttcaaag gatttatttt ttccagtatc gttgcagtta
     2581 ttgccagcta tgcttttgca gcattccaag ttaagcgtat caatcggtta aggaatgcga
     2641 cgaaagaagt gacgaatggt aattttgatg tacagctccc cgtacatgac aaagatgaat
     2701 ttgatgagtt agcagatgat ttcaataaaa tgacgaattc attgaaagaa tctcaagcag
     2761 aaattgaaga acaagaaaat cgtcgaagac aatttatggc agatgcttct cacgagatga
     2821 gaacacctct tacgacaatc aacggacttc tggaagggtt agaatacaat gcaatccctg
     2881 aaaatcagcg agaaaacgcg attaaattga tgaaaaatga aacagagcgt ctgatccgtt
     2941 tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta
     3001 aaaaattcaa tggaacagaa acattgaaaa atattgttgc acagttagaa gctaaagcag
     3061 aagcagcagg agatacattg acattgaaag ccgttgatga tattgatgtt tacgctgatt
     3121 atgaccgatt tgtccaaatc atggtcaata ttattcaaaa cgcgatacag tttactgaaa
     3181 acggccaaat cacgattact ttagaaaaag gctatcttga aacgatcatc acgattgaag
     3241 atacgggaat tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt tattataaag
     3301 tagatccttc aagaaaaaat acaaaatatg gtgaatcagg attaggttta ccaatcgtgc
     3361 agcaacttgt acgcttgcat aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa
     3421 caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta
     3481 taaaagatca tgaatcgatc ttctataccg cttgttttta ctataaaatg attgatttag
     3541 cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc atgagtaaag
     3601 agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag tagctcttgg
     3661 atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt tcgcttggaa
     3721 agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc agctgccgca
     3781 caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg atcttgtttg
     3841 atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata aggaaacatc
     3901 ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac ctcttttttc
     3961 aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt caagtgttca
     4021 gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca aatgatggat
     4081 aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc caataagagt
     4141 ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa
     4201 aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga tatcacggat
     4261 atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa gcgcttcata
     4321 aactgactgg aagctgacga tccacaataa aaccaagata caacaaagaa agagaggaaa
     4381 gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag tcaggcgcca
     4441 attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa
     4501 ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag ctaattctaa
     4561 agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat cttggacttg
     4621 attggctaaa gccgattgcg aatggtaaag agtcaggcat gctatcaata aaaaagcaga
     4681 aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta atcgagcagt
     4741 agcatagcgg attattttca tacttttcac ctctataaaa agtatagaaa atgaatatga
     4801 ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa
     4861 agcaagaggt tgtgaagaag ctgccctatt ctattaccgt agatgcaagc ttttgaacac
     4921 tgtttattcg aaaagaggag cagcgacaat aatttt
//
LOCUS       DAYYAR010000165         4934 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000165 DAYYAR010000000
VERSION     DAYYAR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.168"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..1021)
                     /gene="lacD"
                     /locus_tag="ACHAT0_002612"
     CDS             complement(47..1021)
                     /gene="lacD"
                     /locus_tag="ACHAT0_002612"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8452508.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(1018..1476)
                     /locus_tag="ACHAT0_002613"
     CDS             complement(1018..1476)
                     /locus_tag="ACHAT0_002613"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8452509.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(1473..2936)
                     /locus_tag="ACHAT0_002614"
     CDS             complement(1473..2936)
                     /locus_tag="ACHAT0_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8452510.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(2918..3868)
                     /gene="pfkB"
                     /locus_tag="ACHAT0_002615"
     CDS             complement(2918..3868)
                     /gene="pfkB"
                     /locus_tag="ACHAT0_002615"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8452511.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            4011..4760
                     /locus_tag="ACHAT0_002616"
     CDS             4011..4760
                     /locus_tag="ACHAT0_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8452512.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT         1478 a         1089 c          798 g         1569 t
ORIGIN      
        1 gctatctaat tgcagcagca tctttttatg aagaaacaac tttttcttat ctatcaacgt
       61 agacaggtgt tgctgtttct tctaatagtg tacttaattc tttaatattt tgttttcctt
      121 cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt gccactccct
      181 tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat tgttttgcaa
      241 aacgtaatgt atcttgaaat agcgtagcat tgactcccgc acttaaaaaa ataaacggtt
      301 gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga ctataacaag
      361 tctcttgccc atatccttcc acaaatctca tattcactgg cacttctact ttcaatacat
      421 ccacttgata acgttctttt gaaaattctc gcatggcttc aataacttta tgtggtttta
      481 cttttgcata ggcaagagaa gacggatcgc caatagttgc atcataagaa agaatctcta
      541 aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata actatttgtt
      601 tttgcacatt aatctctgct ggttcatcaa cgtcatagta caataaaaat ttacaagcat
      661 ccgcaccagc ttcttttaac cttttcaccg accaaatatt caataaatca ggtaatcttc
      721 ccggtatttt cgtatcatag ccggtttttt cataagctag cagtaagcca cattctttct
      781 ctcgaagcgc tgctgcaggc aggccgtatt ctggatcaag taaaatagct gaggcatagg
      841 gagtcaggta ttgagaaacc atctgtttaa attggatgat gtcttctgat gtagcttctt
      901 tatattggtt gatcattttc ttcaaagcac cacgctggtc gattgctaat gcgctaataa
      961 ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc atcttcttca
     1021 tagatcttca cctatttctt tagttaatat tgtggctatt tctgtttttg attttgcttt
     1081 tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag acaataattt
     1141 caaatgagtc gttcctgctt ctgaatctgg aataaataac gcaaaaataa aatcaactga
     1201 ctgtctatct aaactgttcc attcaattcc tttgttcagt gtggcgataa tcatactagg
     1261 ttcgttgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta ttcctgtagt
     1321 cccttctttt tctctttcta gaagtttttg atatacttct ttttcgtcaa gagcgatccc
     1381 taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgactat ccaatggctg
     1441 ttgtaaaaaa atgttctctt catgaatgat tgtcatgata agtctcctgc tcttcctttt
     1501 tcgataactg tacgatttgc tgctttatta tccatcttct tgtctttatc agttactctt
     1561 ttcttttgga ttaatcctag tatcactcca ccaacgatcg aaccaataag aatcgctgta
     1621 acccattgga tttttcccgt cactacaggt aacactaaaa aaccaccatg tggtgctgga
     1681 acttggacgc caaacaaata agttaagaca gcagcaattg atgagcctaa catcatcgtg
     1741 ggtaacactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt aatatgagtt
     1801 gatcctaaaa taaaattaac caaacctgca ttacgatcat tggtatcaaa atatttacca
     1861 aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tacaagctgc tgacacacca
     1921 gccataaaag tcgtgtttcc ttgtgccaaa agagctgttc ctgttacata tgccgcctta
     1981 ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc aagaacaagt
     2041 ggattcgaat gttcaaatcc agctaaaaag ctcatcattc cttcatttat agccttcatt
     2101 ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac tggatataag
     2161 aagattgcct tcaagccatc tagttgttta ggtaaaattg tcagtagctt ttgcaaaaat
     2221 aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc agtaccgcca
     2281 ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt cgcgatccct
     2341 tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc gccaactgct
     2401 tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcaaaaga atagattccc
     2461 caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg caacatatga
     2521 gagacaccgt tcattaaatt tttatagatg ctacgaccaa aattagcacc actcatcgct
     2581 tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt tcctgccaat
     2641 gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac atcaatgact
     2701 cgttttcccg caaatctttc tgaatggaca tctttatctg cggcaataat gacagcatct
     2761 gcctgtttga tttcttcagc cgttaattca ttttcgatcc caatttgtcc atgggtctcc
     2821 actttgatct tgatgccttt tttcctagct gcttgttcta atgcctcttg cgccatgtaa
     2881 gtatgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt tcccatgttt
     2941 caaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc aaaaaatcag
     3001 tcagtccttt acgaaaagcg gttgagctgc ctgctgctat gcttttacgt aacgtttcat
     3061 caagcggtcg tcgattcata tatcctgcta aaaaagcgcc taacatggta tcacctgcac
     3121 atgctgtatt gacaactttt ccttttggcg agtttcctcg aaacaccctg tctttcgtaa
     3181 aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcacct aactctacta
     3241 gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg aaccaagaag
     3301 aaagttcttc ctcgttcggt ttcagcaaaa acggtcgata gggtaaacaa tccatggctt
     3361 cttgatcact actatcaata atcaaatgaa tccccttttc ctgacaaatc cgactgattt
     3421 ctattaaaac ctttggagac agccctcttg gtaaactccc agaaacacaa agataatctt
     3481 ctgcttgtaa tgctcttatt tgatttaaaa aattatggac agctgtctgc ggtattttag
     3541 gtccttgatt cactaatttg tattcttctc ccgtttgatt gacttgtgta aatacattga
     3601 tacgcgtcat accaggcact tcaataaact ctgtttggat ttgtttctgc tgtaaatagt
     3661 cttctatata ctttcctgta aacccagcag aaaaacctaa tgccgtactt gggaaatcta
     3721 gcatttttaa tactagtgac acattcaccc cttttccgtt tgcctgaatg tcatcctctt
     3781 ttgtgcggtt taccataaaa ggatgcatct cctctgtttc aataaataga tcaattgcca
     3841 aattcatcgt gcatgtgtag atagacataa ccaacgacct ccttgactct attatgaaat
     3901 acgcgaaaga gaaaatgttt tcttaatcgt cccaacacaa agaaaacctt tacatttata
     3961 aactcgtcgt gctatttttt aactaacttt tggtgaaaga aggaaagaat atggatcaca
     4021 aattaagtga aacagaacag tacctctgga actttatcga acaccacatc ctagagatcc
     4081 ctaactactc catcgtgaaa ctaagtgaac aagcaaatgt ctcaacagcc accattgtgc
     4141 ggacaatgaa aaagaaaggc tatgaaggat ttacttcgtt taagcaccat ctaaaagaag
     4201 gagaaaacaa gaacatcaat ttttctttct tagataaagt agacaaaggt atccgtcgag
     4261 ccattctaaa aaatgaacaa gaagtggtac gtacgatcaa tatgttggaa atcggaaata
     4321 ttgaggatgc aatacaaaga atcaaatttg ctgaccgggt ttttgtcttt gcacgaggat
     4381 tttcagaaat gattggtcaa gagatgctcg taaaacttca attaacaggg aaaaattgtc
     4441 aaatgcatac tgatccagaa atcatcaaaa gcatcagtcg gaaactaact aagaaagaca
     4501 gcgttatttt tgtttctttg aacggcgaga caaaagaact cgtcaccgcc gcaaagaatt
     4561 gctatgatgc agagattggt tcgattctag ttacagccaa tcattcctct acactgaaag
     4621 actattgcga gatcaatctc gtaggtttca aatcagaagg ttcttatttc cctgattatg
     4681 aagtacggtc acgtttgcct ctccagatta tagcgagaat cctgcttgac gcctatgtat
     4741 tgcgtatggg ggagaattga aaagcagttt attatggttg gttacagttt aaaacatcac
     4801 attttttatt ctttatattg tatggactca cggaaacttt ttatgtataa agttattcac
     4861 aatctataaa gttactttaa ataacaaaaa aataattgaa aatacttaaa atcaaaaaat
     4921 atctttttaa tcaa
//
LOCUS       DAYYAR010000166         4876 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000166 DAYYAR010000000
VERSION     DAYYAR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.169"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..1177)
                     /gene="xerS"
                     /locus_tag="ACHAT0_002617"
     CDS             complement(92..1177)
                     /gene="xerS"
                     /locus_tag="ACHAT0_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8452513.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1526..1721
                     /locus_tag="ACHAT0_002618"
                     /pseudo
     CDS             1526..1721
                     /locus_tag="ACHAT0_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3253..3771)
                     /locus_tag="ACHAT0_002619"
     CDS             complement(3253..3771)
                     /locus_tag="ACHAT0_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8452514.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4138..4386
                     /locus_tag="ACHAT0_002620"
     CDS             4138..4386
                     /locus_tag="ACHAT0_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452515.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1610 a          795 c          743 g         1728 t
ORIGIN      
        1 atataaaaat gataatgaag cttgccttta aagttgggct tttatctaat tttagagaca
       61 ggcttcatta ttatactatt ttttactaca tttacaaagc gttcaaagca tcaattgtag
      121 ctgcattgtc tatatgagta tataaagttg ttgtatttgt acttgtatgt cccaattgtt
      181 gggcagttaa taccaatgaa tttgtctgtt gatagagtct agtagccaaa gtatgacgta
      241 atttatgtgg actgacttgg actctgaatc cttttgaata ttttgctacc atccgttcga
      301 ttgcaatacc ggatatacgc ttggcttctc cttgataaat tgctagaaat aaagcttttt
      361 cgttttctgt tactttgtat cgttctttcc gaatagctag ataattagct aaatattcca
      421 ttgcaaatgc tgctatattt actgcgtctc tcttatttcc ttttctagtt actaccacat
      481 tattcttttc taaagatagg tcctctaaat taatatttac aacctcggag agtcttaatc
      541 cagatcctaa aatcaatgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
      601 tcaaagctcg tttgctcaaa gtttttttat attcattggc aatataatct agataatttt
      661 gtgtatcttc gtctagaaat aattttttct cgattgcgtt tgcacgggca gctaacgtag
      721 ttttatctgt gatagttgct acttgatcca taggattatt ttctaagtat ggttttccta
      781 aaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgct gtgatatttc
      841 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
      901 tctctttttt tacattttcc tttgtgcgac gtttcaaatg agatttgtac aagtttagat
      961 cataggcagt taactgtctt atcgaggtga ttgaagtatc gggaacgata gactcactca
     1021 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtagtt cgaggtgatt
     1081 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     1141 ttgtatctat tttttgatat atttctgtat ttttcatgtt attcatcctt tcgataagta
     1201 ggggagagga gtatctctat aattggacta taccaattat agagtgtttt gtcaattggt
     1261 atactctgaa atttattaat ttatttcaaa aaagcagata aataggtata gatttaataa
     1321 tttaaaataa atggtataca tgaataggaa aggaaacaga aggaggcaat cttaattttt
     1381 taaattgtct aaattatata gaaagaacaa tctttctata taattagaca taagagagac
     1441 tacggttgat ttgatattaa caagcgtata tgaatgtata tacaaataaa aaaggggagg
     1501 gagaagttaa agaaaattca gagaagtggg gcactcagta gtattaacaa tttaaaagga
     1561 atttcttaaa gaaaatgggt tgcatcctgg agatacaatt tttttagata aagaaaaaat
     1621 tcaagcaaca attcaaaaaa atcttaactg ctgagattga tgtagcaata tctcaatcat
     1681 caacaaagta cgatcctgcc tttcaaaagc ttattgataa atgtttattc attataaaaa
     1741 taaaagaaaa atttatgtca gcttttttca tcaaagtggt aatgataatt cgtctattaa
     1801 ttgtattttt taggaaatat ggtataattg ttttaacgaa aaggtaattc atgatacacc
     1861 aaaaaccact agctggcggg ctagtggttt tcttttacgt tctattcaga ttaacgggta
     1921 ggcttactgt cttttttggc aataattttg caatcaagcc atttacagat gtaatgcgcg
     1981 gatacttctg ctaagattgc aaaaaacata tcgaaaaggt aattcataat acaacctcct
     2041 ccccttgcca taataggccg gggtagcaga cagcataaat attataccat aactgaaaat
     2101 ttcattttag tacatttcaa tatattgatg aaccagcaca ataaaacatg ccatgactaa
     2161 cagaaaaaaa cttattaaac cagatataac ttgtaaacat ccaactatat agaaaacgta
     2221 aattttctat atagttggat taagataacg actatttatt ttatcatatt tttactatac
     2281 cctatacttt tacttggtat atttatgaat aatcgcaaat ttacccctat actactagtt
     2341 tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa
     2401 cttataaatt gatggtattt agataacacg aaagaataaa aataacagct gatagtaaag
     2461 tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa
     2521 acttatgaaa tgaaagacct tttacatctt gcagaaatcg ttactgggat taaactaagc
     2581 aataaaaact aatgttcccc tcattctttg aacttctact atttgattta tttattctga
     2641 taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct
     2701 tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta gaataaactg
     2761 ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata
     2821 aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga
     2881 tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg
     2941 aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg
     3001 ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt atcgaagtga
     3061 gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg
     3121 cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta
     3181 tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca
     3241 gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc
     3301 aaatggagca actgtattta catgaataaa tttatatact tcccatacag ccgttttggt
     3361 tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc
     3421 aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg
     3481 tagatgtgca taggtatttg taaaccactt atataactct cctaactgat tccatttaaa
     3541 ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat
     3601 taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc
     3661 aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata
     3721 agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc
     3781 tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat
     3841 ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatgatttat
     3901 aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac
     3961 gaatatattt cttttgtagt aaaatcggac tggtttgcat atcattttct tcgtaaatat
     4021 caaaatcatg ttaactacta tcaattacta ataaaaacta ccactttaag cgttaagaac
     4081 tgttaagttt agttaagtga ggatagttca caaaatccat tgttaaactt cacgaaagtg
     4141 gtaaaactca aaatgcttta tataggatta cagcatcgct ctttcttcga ttgcgcgttg
     4201 ggtaaaaaat attatgaagt aaaaattgac gatgacacgt tccttacggc tcatcagatg
     4261 aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa aaagcgattg
     4321 ccatattcac gcctcgctcg acgaactatt aaaggtcaag atttacctga agatcatcct
     4381 ttttaggaaa ttgaagcttt ttatgttgca caatgcattt ataatactac aatgatgttt
     4441 tcaccagata ttcttctagg gtgcggtgta atgaaacaga tacacttgca aaagaaaatt
     4501 caccgcgaat ttactaaaat tatgaataat tatgttaatc ttccgaaatt tttttccaaa
     4561 attaagggat caagctggtg ttatagattg tttaaggttg gcaaaaaaat aacttctttt
     4621 taaacaatta tagaaaaccg atactctgtt aagttggttg acacggtgtc gatttttatg
     4681 actttcttat tctgaaactt cactaacgga cagtacctaa aaagtctata gtatggacgt
     4741 ttacgagtac tattttatca aaaatgctgc tcatttcaac ttatatgttt tagtgaacta
     4801 cccccactta gcttggatag gctacaatta actagacaga aaaattaagg tgtgtagact
     4861 agaagaaaac atacca
//
LOCUS       DAYYAR010000167         4859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000167 DAYYAR010000000
VERSION     DAYYAR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.170"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..1695)
                     /locus_tag="ACHAT0_002621"
     CDS             complement(85..1695)
                     /locus_tag="ACHAT0_002621"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8452516.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1917..2162)
                     /locus_tag="ACHAT0_002622"
     CDS             complement(1917..2162)
                     /locus_tag="ACHAT0_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452517.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2888..4228)
                     /gene="glnA"
                     /locus_tag="ACHAT0_002623"
     CDS             complement(2888..4228)
                     /gene="glnA"
                     /locus_tag="ACHAT0_002623"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8452518.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4256..4648)
                     /locus_tag="ACHAT0_002624"
     CDS             complement(4256..4648)
                     /locus_tag="ACHAT0_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8452519.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1432 a         1046 c          797 g         1584 t
ORIGIN      
        1 aacaaatcag aaaaagagaa aagtgacaag aaaattgctt atccccttct cttgctactt
       61 ttctctttct atcaagttca aaaattattt tgtttcgtga tctgctaatg atgctccgac
      121 aaatgctttg attaattttt gcgggcgatt tggacgagaa atcaattctg ggtggaattg
      181 gcatcccaca aagaattttt tctctggcag ctctacgatt tctactaaac ggttatctgg
      241 tgagacacct gaaaagacta gcccttgttc ttcaaacaat tgacgatatt tgttattgaa
      301 ctcatagcgg tgacggtgac gttcttgcac aacatcggct ccatcatatg cttcagccgt
      361 cttcgttcct tttttgattt tacatgggta taatccaagg cgtaaagttc cacccaaatt
      421 ctcgatgttt tcctgatcag ccattaaatc aataatattg tttgctgtat ctggattcgt
      481 ttcagctgat gacgcatctt ctaatccaac gacgttacgt gcaaattcga cacaagccat
      541 ctgcatacca agacaaatac ctaaaaacgg aacgtcattt tcgcgagcat aacgaatcgc
      601 ttcgattttc ccttctactc cgcgatcacc aaatccacca ggaaccaaga ttccatctgc
      661 gtctttcaaa cgttcttcta cattttctct cgttaattct tgtgctttga tccaatcgat
      721 ctcgatgtct gaattatagg cgaaaccaga atgtttcaat gcttcaacta ctgagatata
      781 tgcatctggt aactcgacat atttaccgac aagcgcaatc cgtacttttt tcttcaagtt
      841 caagacacgt tcttctaatt ctttccattc tgtcatgtct gcttctggtg tatctagttt
      901 taaatggtcg caaacaattt gatccattcc ttgtgcttgt aaaactaatg ggatggagta
      961 aagtgtatct acatctggcg actcgataac tgcttctggt tcgacatcac aaaattgtgc
     1021 caatttgttt ttcacgcttt gagaaacagg ttgctctgta cgtaccacca aaatattcgg
     1081 ttggatacct aaaccacgta gctcttttac actgtgctgc gttggtttag ttttcatttc
     1141 tccagcagct ttcaagtaag gaatcaatgt tgtatggata tacataacat tatctgtacc
     1201 gacatctgct ttcatttgac gaagtgcttc aaggaatggc aaagattcta tatcccctac
     1261 tgttccccct acttctgtaa tgatcacatc tgaatcagtc attttagctg ctcgcatgat
     1321 tttttctttg atctcatttg taatatgagg aataacttga acagttgctc ccaagtattc
     1381 gcccttacgt tctttacgca aaacttcaga atagattttt cctgtcgtca cgttggagta
     1441 tttgttcaaa ttgatgtcaa taaaacgttc gtagtggccc agatccaaat ctgtttcagc
     1501 accatcatct gttacgaata cttctccgtg ctgatatggg ctcatcgtcc ccggatccac
     1561 gttgatgtat ggatcaaatt tttggatagt tacttttaag ccgcggtttt tcaataagcg
     1621 tcctaaagaa gctgcaacaa tcccttttcc gattgatgaa acgacaccgc cggtcacaaa
     1681 aatatatttt gtcataagtt caaaaactcc tttttaattt atgtgcagga aaagtctcag
     1741 acaataaaca aagctcccta ttcttcaata agaataggga gccgaaattt ctgtaaactt
     1801 ctgaccctct aaaagggtgc ccaaggtgta taatacaagc tgactaaggg aaagtcaagt
     1861 actcaaatga atactatccg cttacaattt tttctttttc gatatccttt gagaaattat
     1921 ttcttaagtc ctttatgagt gcaaaaaaaa tttaacccac ataataagct tgatataaac
     1981 atagctaacg ttaaacctaa gcaaatcaga aaatcgttac tgaaaaattt taagtgaaat
     2041 aatagataaa aaaataaaac aaacattatg atagataata tcccacctaa caaagaacta
     2101 ctttttccta cttgcagcaa cagtttatat agcttatcgt agaaacaatc tttttctttc
     2161 acccttattt acctccttta cgtaaaaaac gtagtccaca gttctcggat ttttggcata
     2221 tcttttctat taaataatat caaaaacaaa acaattccac taattttttc aaagatgacc
     2281 tttttttcaa caaaagtgcc ttgtagggat tcattgaagg taacgaatca cataagtaaa
     2341 aaataaggag atgcgacaaa agtcgtgcct ccttatttct aaataaacgg tgttctcctg
     2401 ccaactcctc ggtcacaagt cacaatatcc atcaaacatg agaagctgtt tttgaaatta
     2461 tttcttgtga ttcggagtta aatggtaggc tcacaacctc tggataaacg gtattcaaaa
     2521 gccagtttct cggtattaag ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa
     2581 tttttcttaa tactcgaaac taaacgtctt tttcaaaacc tcttggtaaa cggtgttcaa
     2641 aagcttgcat ctgcggtaat aagttcagcc aattcaaaaa caagaaaagc tgtttttgct
     2701 tggctattct tattacttgt tgcagggcag ctttttcaca acctctccac taaacggtgt
     2761 tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga actatttttc
     2821 cgttagtttc tctaatactc aggtcaaaac gctgatttca cagcctctaa ttcacggtat
     2881 tcctgtttta ataaagttct agatattgtt ctctttccca ctcggaaaca gtttgtctga
     2941 atgccgccca ttcgagacgt tttgcttcaa caaaatttga gaagatatgt tgtcctaatg
     3001 cgtcaatcat tacattatcc ttacgcaatt ctttgatagc attatggata gttgaaggta
     3061 agtcctggat atgtgcagct tctctttctt cctcatccat tacataaatg ttacggtcaa
     3121 cagctgctgg cggtgtgatc tgattacgga taccgtctag tcctgcttgc aacaatactg
     3181 ccattgccaa gtatgggttt gcagatggat caactgaccg caattccaaa cgtgtggata
     3241 aaccgcgaga ttctggtaca cgtactaatg gcgaacgatt acggccactc catgcgacat
     3301 aaacaggcgc ttcataacct ggaacgagac gtttataaga attgactgtt ggattgcaaa
     3361 cggctgtgta ggcacgagca tgtttcagca aaccacctaa gaaatgataa gctgtttcac
     3421 ttagttccaa ttcaccgtct ttatcataga aaacatttcc ttcatcattg aacaaagaca
     3481 tattgcaatg cataccagaa ccattgatcc caaataacgg tttcggcatg aaagtcgcgt
     3541 gaagtccatg cttgcgtgca atcgttttga caacgagctt gaaggtttgg atattatcac
     3601 aagcatctac cacatctgca tatttaaagt caatttcatg ctgtcctggc gctacttcat
     3661 gatgtgaagc ttccacttca aagcctagac tttctaattc caacacgatg tcacggcggc
     3721 aattttcacc taaatctgtt ggtgcaaaat caaaataacc acctttatca ttcaaggtcg
     3781 ttgtgatccc accgtcttcg tctaatttaa ataagaagaa ttctggttca ggtcctaaat
     3841 tgaaagaggt aaaaccaagg tcgcgcatat ctttcaatgc tcgtttcaaa ttcccgcgag
     3901 gatcgccggc aaatggtgtc ccatccggat tatagatatc acaaattaaa cgagcaactt
     3961 ttccatgagc actttcccac gggaagatca tccaagtgga gagatctgga tacaaataca
     4021 tatcgctttc ttcaattcga acaaatcctt caatcgaaga accgtcaaac atcattttat
     4081 tttctaatac tttgtcgatc tggctcactg gaacttctac atttttgatc gtacctaaga
     4141 tgtctgtgaa catcaatcgt aaaaatcgta cgttttcttc ctcaatgatt cgtttgattt
     4201 cttcacctgt tatctgtttc tttaccatgg tatccctctt ccttttttaa tttattcagt
     4261 caaagaattg tctttgcaat ccttgcgact gataatgacc tgttttgctt aacccacctt
     4321 gcgccaaaat ctcatcacga aaaatttgtc gaacgtcatg atctgtcaac ggcttcttgt
     4381 tttttgcttc ttgttttttc gcctgtgcca tttcgtatac acgtttgatc ccagccatat
     4441 tcaatccctc tgaaagatag tctttaatct caagcaacat atcaatatca ttcagcgaat
     4501 acatacgtcg gtttccttcg cttcgttccg gatgaactaa atcttgttct tcataatagc
     4561 gaatttgccg agcagacaaa tcagtcagct tcataactgt accaattgga aatacggaca
     4621 tagatcggcg caattccttt tctcccatca gacgtcccct ttcgttttat aagttggcta
     4681 aagtgtacca attaaaaaaa atattgtcaa ctgcatatgt taggttttct aacatgagat
     4741 ctctattttt tataaaataa caaaaaaata agaacacaaa aaaggtatct agttgatttt
     4801 cgtaccagat accttttatt ttttattata ctgtactatc ctttattagt gtttttatt
//
LOCUS       DAYYAR010000168         4833 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000168 DAYYAR010000000
VERSION     DAYYAR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.171"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..675
                     /locus_tag="ACHAT0_002625"
     CDS             <1..675
                     /locus_tag="ACHAT0_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8452520.1"
                     /translation="LSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLEL
                     LGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMI
                     FAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQ
                     VLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKY
                     YPGKGQ"
     gene            677..1621
                     /locus_tag="ACHAT0_002626"
     CDS             677..1621
                     /locus_tag="ACHAT0_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8452521.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1820..2464
                     /gene="pcp"
                     /locus_tag="ACHAT0_002627"
     CDS             1820..2464
                     /gene="pcp"
                     /locus_tag="ACHAT0_002627"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8452522.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2543..3820
                     /locus_tag="ACHAT0_002628"
     CDS             2543..3820
                     /locus_tag="ACHAT0_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452523.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            4078..>4833
                     /locus_tag="ACHAT0_002629"
     CDS             4078..>4833
                     /locus_tag="ACHAT0_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8452524.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         1557 a          885 c         1039 g         1352 t
ORIGIN      
        1 cttagccttt taggcgtctt gattgttatt ctcgggtttg catttaaatt agactcgata
       61 ttgatcgtaa tggttgcttt agttgttaca gctttgactg gcggtcttgg cattcaagga
      121 atgttagaac ttttaggtac aagttttgta aacaatcgcg ggatggcgat ttttatcatc
      181 attatgttgg caacaggaac gcttgaaaga aatggtctga aagaatcagc ggcttccttg
      241 atcaagcgat tcaagaaagt atctgctggt atgattgtag atatttatgg gatctttcga
      301 atgatctttg cggcttttaa cgtgagtttt ggtggagttg ctggatttgt tcgtccaatc
      361 attttaccga tggcattggg gaccatcgaa tcaaaagggt tagaagtcca tcctaagcat
      421 gaagaagaac taaaaggtat ggcttcagct atggaaaatg tctgctggtt ctttggccaa
      481 gtactattca tcggaggagc aggggcgttg ctagtccaat caacactgaa agatctagga
      541 tatgacgtaa cattgggaca gcttgcttta gtggaagttc cagtggctat cacggcttta
      601 gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat tagcgaagaa atattatcca
      661 ggaaaggggc aataagatgg acttttttac aaatcctgat attttattag gcgataaatt
      721 actggagatc ctttatatcg tgatgggact cgtattgatt tacactggag taagaaattt
      781 aacagataaa accaatcctc atcgttatgg gtcagccttt ttctggacgg tcttaggggt
      841 agttattgca ggaggaagat ggcttccggc aatcgtgaac ggtggcttga tctttgcgat
      901 gaccatacca gcaattgcta agaaagtcag caaaggagaa agccggcttc catcaaaaga
      961 atatatggaa aaaatgtcag acaaactagg aatgaaaatt tttattccag ctttgagtat
     1021 cggtgtcttt gcgatcttgt ttgctctatt taccaattta ggtgctttag tcggcgtggg
     1081 cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt tctcgagata atcgaccaag
     1141 tacattttta gatgatgcag cagatatgct gggcacagta gggccgttaa gcatgttgcc
     1201 aatgctttta gcttctctag gtgcggtatt tacaagcgct ggagttggga cagtgatttc
     1261 caatgcagta ggtacgatcg ttccagaagg aaatgtgaac atcggcatca tcatttacgc
     1321 tcttggcatg gtgatattta cggtaatcat ggggaatgca tttgcggcca tcaccgtgat
     1381 gacggttggg atcggtgcac cattcgtatt tagtcatgga gcgaatccag cgttaatagg
     1441 aatggttgca ttgacttgcg gtttttgtgg gaccctgctg acaccgatgg ctgctaactt
     1501 caatatcgtg cctgtagcga tgctggagat gaaagacaaa tacggagtga tcaaaaacca
     1561 gttgtttatt gcgttattta tgctagtttt ccaaattgct tatatgattc tatttaaata
     1621 atagaagaga ggggttgtga cagaataatt gtcgcaacct cttctaacat tgtagtggaa
     1681 cactattttt ttataagaat atattaagtt tgatctgtta tgttatttaa actattgttt
     1741 cctttattta cgctgttcaa ggaaaatggt atgataaaag ataaccatat gttaaatcaa
     1801 tcggacggag gaacaacaga tgaaagtact agtaacaggt tttgatccat ttggtgggga
     1861 caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca gatgaaatca gcggtgctga
     1921 aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc agtcaagtag tcaaagaagc
     1981 catccaacag caccaaccag atgtcgtgat ctgtgtcgga caagcaggtg ggcggtcagc
     2041 agtctcgttc gaacgtgtcg caatcaatct agcagaagca agaatccctg ataatgaagg
     2101 aaatcagcca ttcgatacgg ctttagaaga aaacggtcca gccgcttact ttacctcatt
     2161 gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt ctaccagcgt acatctctta
     2221 tacagcagga acatttgtct gcaatgatat catgtatcgt ttgcttcatt tgatcgaaac
     2281 acaattcccg gcaattcgag gtgggttcat ccacgtgcca ttctcacccg atcaagtgat
     2341 cgatcgtcca gtgggtacgc cttctatgtc tttagaagac attgcttctt ctttgactta
     2401 tgcagttgaa gcagcagtag aaaataaaga agatatttcc ggaaatgccg gaacaactca
     2461 ctaatttagc aagaaacact ggctggttgc taacgttatg ttagtagcca gctttttgta
     2521 attagaaagg aaagaaacag ccgtggaaga atggcaatcc gtctttgaag aatggtttcc
     2581 aaaagaaatc agcaaaagct atcccatcaa aatttctaaa caatacacaa gcagtcaacg
     2641 ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa ctagtcgata agcaccgccg
     2701 atatctaatc agctctcgtt ttatggaaga acattattta gctgcaacag attgggtgtt
     2761 ttctgacttc aagatcaatc cgttttttcg aacaaaacga agccagcaaa aattgtattg
     2821 tgagtgtggt agagaattaa aagtgcaata catcgttaaa tcgccaaaaa ccggaaagat
     2881 cctaaaacta ggcatcaacc actttgccga tcacctgcat gtctctccga cagtggcagc
     2941 ttcgatccat cagggaatga caaaagttga tctggcctta gacgaattat tatggcttaa
     3001 gcagaagaat atcgattttc ctgaaggact atggcaaaaa tactgttttg tcttgtacca
     3061 aaatcgtcgg atgaaacagc catatttacc ggatataaaa ttagctcagc ggctcgcaga
     3121 atttcgccaa gtagaaatgc cgatttacat agcggattat caagcattgg aaaatgaaat
     3181 caagaaaata agtgaacaca tcaatgggca gtcaaaaaag cgacagataa aaaaagaact
     3241 atttgacgac tttgcagaag aattagtaaa ggatgtagaa gaattcttga tcaactatcg
     3301 cgcattttta cgtaaagatt ggcaatccat cgtatatgaa gaagtgccag tacatcctaa
     3361 tgcttatttt gaaacattta tttccgtgtt gagaaagaca aaacgtcaac gaacaccaga
     3421 agtcactgct cagatggaat attttgcgaa aaaccaacga ttcatccaac caaaaatcta
     3481 tctatttatt tggaagcaat attgtcgcta tggttttaca gaagggttct tcgacagcat
     3541 tcctcggata gtcagaaatg gttttttaaa agtattgcgt aaagaacgag aagctataca
     3601 atctgctgat aagaaagacc gtacagtctc aaaagaaaaa tggcaactag tagtgaaaga
     3661 tatccaatct ggaaatgtgc aagagacaat cgacaaatgg aaaggaaaac attatcgttt
     3721 tactgaagct cagaaacaag cgttggaata ttatcagaag ttagaagaat cactgcgttt
     3781 caatgatgag gcaagaaagt atctgaagga attactataa aaaacatact aagattagag
     3841 gggaacatca ttgacgaatg atgttctcct ctattttcta tttttagatt atagtgaaga
     3901 gtgaaagaaa gttcgaacaa agccttgagg actacgagtc cctatcaaaa accgaacttc
     3961 tttactgtca ttttagaatc aggtttacgt atgttggggc catgagcccg tgtataggaa
     4021 attggaatcg aaatgaagca gtaaagttct ttcaaaactt aaaaaggagg aaaacaaatg
     4081 ccttacattc ttgcattaga tgtttcaatg ggacacagct acagtgtcct atacaaagac
     4141 gacacctgct tatttgaaga cgagattgaa cacactcaaa gaggatttca tgcattactc
     4201 gaagagatcc agcacttgcc tgagcgtcca ttgattgttt ttgaagcaac ggggatttat
     4261 tccagaccag tcgaaaagtt ctttcaagat catcactttt cttactgttt gttaaacccg
     4321 ttagaggcga aaaaacagat ggaagaaagc accttacgca gttggaaaac agataaatcg
     4381 gatgcccacc ggctcgcaca gactcatctt aaaaatcaac gtcagccaaa agaagtacaa
     4441 agcaattttt acttagagat gcgcgattta gcccgttttt atcaagaaat tgagaagaaa
     4501 atcactcgtc tgcgcatgga tttacacaac tgccttcaat tgacttttcc agagcttgaa
     4561 cagttctttt caaacaggct gaccccttac gcattgacat tgatccgtct ttttccccat
     4621 ccagattttg tattggcatc tactcggaca aaaataaaaa ataaactgat caacgaaaca
     4681 cggaaaaaaa tctccgcgaa tcgtgccgag caaaaggcag atcaaatcat ccactatgca
     4741 cagtgtgcct atccagctgt tgaaaaagac agtatccact gtcaaaaaac gatctattac
     4801 gccgaacttc tccaagattt actcgaacaa aaa
//
LOCUS       DAYYAR010000169         4726 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000169 DAYYAR010000000
VERSION     DAYYAR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.172"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..1062)
                     /gene="whiA"
                     /locus_tag="ACHAT0_002630"
     CDS             complement(127..1062)
                     /gene="whiA"
                     /locus_tag="ACHAT0_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8452525.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1096..2094)
                     /gene="yvcK"
                     /locus_tag="ACHAT0_002631"
     CDS             complement(1096..2094)
                     /gene="yvcK"
                     /locus_tag="ACHAT0_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8452526.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2091..2975)
                     /gene="rapZ"
                     /locus_tag="ACHAT0_002632"
     CDS             complement(2091..2975)
                     /gene="rapZ"
                     /locus_tag="ACHAT0_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8452527.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3110..3841)
                     /locus_tag="ACHAT0_002633"
     CDS             complement(3110..3841)
                     /locus_tag="ACHAT0_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8452528.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(3845..>4726)
                     /locus_tag="ACHAT0_002634"
     CDS             complement(3845..>4726)
                     /locus_tag="ACHAT0_002634"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8452529.1"
                     /translation="FYEVCEEYDLPYPKTLIVREEMLVNGHLEQELPFEFPVALKPAN
                     SVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFIPGDDSNMRVLNA
                     YVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKAFLEKIEYTGFAN
                     FDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFNKPFVETTYGTNQ
                     SDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKDRSIKRWLLMKYM
                     FHNYIPRFKKYFHVKEG"
BASE COUNT         1311 a         1037 c          804 g         1574 t
ORIGIN      
        1 cctttccaaa ttatgttact ccagtaaaga tggaattata gtttgacgga agaaaagcaa
       61 gtataacttt taaaaaaaac tgatacattc gcatctcctg cttcatgtat cagttttttc
      121 tttggattaa actgcttctt cacgcagttt ttccgcaaat tcatttattt tgcgaattcg
      181 gtgattaatt cccgattttg atatcgcacc tgagggaatc atttccccta attctttcaa
      241 gctgacctct ggatgttgaa gccgaagttc tgcgatttcc tgaagttttt caggaagggc
      301 atgtagaccg acctttgctt cgatcaattc gatgttttcg atttgtttag aagccgcatc
      361 aatcgtcttg ttcagatttg ctgtctcaca attgactaga cgattgaccg aatttctcat
      421 atcccgtacg attcgaacat cttcaaatcg gagcattgag ttggttgcac caatcaatgt
      481 taagaaatca gcaatttttt ctgctccttt taaataagaa atatagccat tgcgccgttc
      541 aagcgttcga gcatgtagac cgtaataatt taacatatcg caaatgtctt ggttatgctg
      601 ttcataaata gaaaatattt ccaaatgata acggctcgtt tccggattat tgactgaacc
      661 tgaggccata aaagcaccac gtaagtaaga acgcattttt tgcgaatttc ccataatctc
      721 gtccgaaacg tgtccattga acatcatacc atccatgata tcaagatcca tcaacacatc
      781 ttttgtgcct tgcttcaacc ggacgatata gacattgttc ttttttaatt tcattttttt
      841 acgaacgagt agctcacttc gtacctgata atgatctttc aaaagagaat agatacgtct
      901 ggcaattgca gcattttctg tttggacgtt caaaataaac tgctggttca caatacctaa
      961 agagccgttc attcgcagca aagcagctaa ttccgctttc gcatgttctc gatgaacttc
     1021 caaaccagtt agttctttct tcacatctgc agcaaatgac atcctattct cctcctttct
     1081 ccaacaataa attgcttaat attttgaacc gaaaactaaa cggaacaatt cttctactac
     1141 tttgtctcca tcgtggaaga caccaccatc tttcaattcc aaaaagtcgg tggaaatcac
     1201 tcgacatcct tcatttctca atccgttgaa gtcgtgtgcg acttgtacta gatactcatc
     1261 atagatctct gggtccatat aatcttcagg cactttttcc gtattgacca atactgtatc
     1321 gataaaaggc tgattcaaat gcttgtgtaa aacacgtata tgatcggcat ctgtgaaatg
     1381 ttccgtttcc cctttttggg tcatgatatt acagatatag acggtttcag cagttgtctt
     1441 gagcattgct tcgccgattt ctggtatcac taaatttgga aggatactgg tgaataaact
     1501 tccgggtccc aaaacgacca tgtcggcgtc cataattgct gaaacgactt ttcgagccgc
     1561 tttggcttca cttttatcat ttgtgttgca gacatacaca cggtcaatgg ttttacggtc
     1621 gatcgcgatt ttagattctc ctactgcagt cgttccgtct ttaaagacag catgcaaggt
     1681 caaaggagtt tctgaagaag gataaacatg tccatctaca tgcatcattt ttgctaataa
     1741 ttgaatcgcc tcataagtgc ttccccgcat ctcggaaact gcggcaatga tcagattacc
     1801 gatcgtatga ttggctaaaa actgatcttc ctttttgaaa cggtattgga aaatatcttc
     1861 atagaattgt ggcatatccg ataacgccac taagacattt ctcaagtcac caggaggagc
     1921 catagcgatc gattctctga ttgcaccgct gcttccccca tcatctgcaa cagtcactac
     1981 tgctgtgata tctgctcctt gatttcgcaa acttttcagg ataactggca gaccagtgcc
     2041 accgccgacc actacgatct ttggttttct tatccggtag gttttcattt tcacgaccgg
     2101 ttcaccgtct ctttcctttt atccttgtcc cgatgtgtca cattgacttt atagtttttc
     2161 cctaaagcat ttgccaaacg ttcagtcaat gccacagaac ggtgttgacc gcctgtacag
     2221 ccgattgcaa ctgtcaagct gctttttcct tctttaatgt atccaggcaa tacagtttcc
     2281 agaagatcca aaaatttatg gtagaatgat tgcgtttcat caaaggacat cacataatca
     2341 tagactggct gatcttttcc ggtcaatggt cttagttccg ggatataatg agggttgggc
     2401 aaaaaacgca catccatcac aatatccgaa tcgatcggca acccgtattt aaaaccaaat
     2461 gatacaaatt cgatccgaaa tccttggtct ccctttgatt tgaatgactc attgattttt
     2521 tcacgtaatt gtcgtggtgt caatgtcgtc gtatcaatga tcacggaagc ttgggctttc
     2581 aagtcgtcta gtatcgcccg ttctttacgt atcccttcag tgatcaaacc gtccatcgcc
     2641 attggatgtg ctcgtcttgt ttctttgtag cgagccacta gttcttcgtc tgaagcatct
     2701 aaaaataaga tagaagtgtc gatgaagttc gtattttcga tttcgatcag catgttttgg
     2761 atttcttcaa agaaggaacg agaacgtaaa tccactccta aagccatttt tgtgactttg
     2821 cctgattctt tgattaattc ccagaatttt ggaatcaatc gaggcggcat attatcgata
     2881 caaaaatagc ccatatcttc aaaactttgg atagctactg ttttccctgc accgctcata
     2941 cctgttatga tgactaattg caaattatct gtcataatta cgaactcctc tcacattgtg
     3001 catagtaaaa ttatatcatt ccgggcgtat cattaccagt tttctttttc ttttacaaaa
     3061 agatttggac caaatatctt ttaggatatt tgccccaaat ccaatttttt tacttttccg
     3121 atgctgtatc caatgccttg cttctttcag ctagcgctcg ttcaatcgtc cgatctgcta
     3181 aaatagattg ggcatcgatc actggataat ggttatcttc agcaaattca tgcatcaatg
     3241 acaattctgt gcaacctaag ataacttttt cacagtttag gcgctctacc gcttcttcta
     3301 agatttcgta atatagctct tgattcaaat aatccgattc cttgatctca tgatagatca
     3361 gataattgat tttttcttga agaacagtat caggaatcac tgtttcgaat ccaagatttt
     3421 tgacttcgcg ttcatagatt ccagctttca tacttccttc tgttcctaga atcgctactc
     3481 tccctgtcgt atgctgacgg acaagttcat ttgctgcttc tcttggcata tgaaggatag
     3541 gaatatccgt tgccgcttgt aattcttcaa aaaagtagtg tgcggtgtta caagtcaaca
     3601 caatgaagtt cggctgcaat aaattttgtt tctcaatatc atccagcaga aacggcattg
     3661 gattttcttc tgatcgatct aaaatataag ctgtacgatc aggaactgtt gcatgattga
     3721 ataagacata attcaaatat tcttgatctt ttgtagcttt cgttcggtgg ttgatcaagc
     3781 gaacaaaact ttctgtcgcc atcgtgccca ttccgcctaa aatactgaaa aaattctcca
     3841 tgaactagcc ttctttcaca tgaaaatact ttttgaatct tggaatatag ttatggaaca
     3901 tatatttcat caacaaccag cgcttgattg accggtcttt ttcataaaac acagtagtac
     3961 cgtaacgatt ttctttgatc atttgctcag ctagtttttt gtcttcattt tcccgagcat
     4021 attctaaaaa gatttttttg ggcacaccca tccataatct tgctttatct gattgattcg
     4081 ttccataggt cgtttcaaca aagggtttgt taaacactcg atcttctgtg acgaaacgag
     4141 ccagattcaa gccgttcaat gtcacaaaga aactgctacg accttgtctc aaattgattt
     4201 caaataattt atattctcca tctctaggat catacttcat atcgaaattg gcaaagcctg
     4261 tatattcgat tttttccaaa aatgccttaa tggtttgata gattttttcg ttataatctg
     4321 gcatgatcac aacgtaatta ccaatcgatg ctggtgtagg atcttctaac agtggatgac
     4381 caagacacat catccgaact tgatgatctt catctacata cgcatttagc acacgcatat
     4441 tactatcatc acccgggata aaatcttgta caatcatttc acttttataa ccggcttcgt
     4501 atatccggcc taaaattaaa tcgaactctt ctctagtctc taaaataaaa gctttttttc
     4561 ttccttcaaa ttggacagaa agatactcca cactgtttgc aggtttcaaa gcaactggga
     4621 attcaaaagg caattcctgt tccaaatgtc catttactaa catttcttct ctgacgatca
     4681 atgttttagg atacggaaga tcatattctt cacaaacttc ataaaa
//
LOCUS       DAYYAR010000170         4690 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000170 DAYYAR010000000
VERSION     DAYYAR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.173"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..996)
                     /locus_tag="ACHAT0_002635"
     CDS             complement(13..996)
                     /locus_tag="ACHAT0_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8452530.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1001..1648)
                     /locus_tag="ACHAT0_002636"
     CDS             complement(1001..1648)
                     /locus_tag="ACHAT0_002636"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8452531.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1733..2365)
                     /locus_tag="ACHAT0_002637"
     CDS             complement(1733..2365)
                     /locus_tag="ACHAT0_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8452532.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="ACHAT0_002638"
     CDS             complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="ACHAT0_002638"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8452533.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2929..3666)
                     /locus_tag="ACHAT0_002639"
     CDS             complement(2929..3666)
                     /locus_tag="ACHAT0_002639"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8452534.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3663..4652)
                     /locus_tag="ACHAT0_002640"
     CDS             complement(3663..4652)
                     /locus_tag="ACHAT0_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8452535.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         1327 a          972 c          924 g         1467 t
ORIGIN      
        1 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
       61 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
      121 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
      181 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
      241 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
      301 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
      361 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
      421 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
      481 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
      541 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
      601 aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
      661 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
      721 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
      781 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
      841 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
      901 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
      961 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     1021 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     1081 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     1141 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     1201 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     1261 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     1321 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     1381 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     1441 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     1501 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     1561 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     1621 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     1681 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     1741 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     1801 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     1861 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     1921 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     1981 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     2041 ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     2101 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     2161 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     2221 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     2281 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     2341 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     2401 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     2461 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     2521 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     2581 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     2641 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     2701 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     2761 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     2821 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     2881 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     2941 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     3001 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     3061 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     3121 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     3181 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     3241 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     3301 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     3361 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     3421 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     3481 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     3541 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     3601 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     3661 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     3721 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     3781 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     3841 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     3901 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     3961 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     4021 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     4081 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg taatgattga ctggaatcct
     4141 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     4201 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     4261 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     4321 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     4381 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     4441 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     4501 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     4561 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     4621 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
     4681 tttatcatac
//
LOCUS       DAYYAR010000171         4678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000171 DAYYAR010000000
VERSION     DAYYAR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.174"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..457
                     /locus_tag="ACHAT0_002641"
     CDS             <1..457
                     /locus_tag="ACHAT0_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8452536.1"
                     /translation="NNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFGETYVLKMP
                     AINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSYENDKMY
                     IIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            469..1473
                     /gene="neuB"
                     /locus_tag="ACHAT0_002642"
     CDS             469..1473
                     /gene="neuB"
                     /locus_tag="ACHAT0_002642"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8452537.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            1470..2618
                     /gene="neuC"
                     /locus_tag="ACHAT0_002643"
     CDS             1470..2618
                     /gene="neuC"
                     /locus_tag="ACHAT0_002643"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8452538.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            2591..3268
                     /locus_tag="ACHAT0_002644"
     CDS             2591..3268
                     /locus_tag="ACHAT0_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8452539.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3219..4400
                     /locus_tag="ACHAT0_002645"
     CDS             3219..4400
                     /locus_tag="ACHAT0_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8452540.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            4413..>4678
                     /locus_tag="ACHAT0_002646"
     CDS             4413..>4678
                     /locus_tag="ACHAT0_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8452541.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNI"
BASE COUNT         1729 a          606 c          917 g         1426 t
ORIGIN      
        1 aaacaataaa gatattactt tgacagatgt tgatatgaca cgatttatga tgtcaatacc
       61 ggatgctgca agattagttc tgtctgctgc tatgaattca aaatttggag aaacatatgt
      121 cttaaaaatg ccggcaatca atattttaac tttagctcaa tcattaataa aaatatataa
      181 tgatacacat gatgaaaaat atagaggtga aataaaagtt gtaggtatca ggccaggaga
      241 aaaattatat gaagagctaa tgactacaga cgaaattgaa cgctcatatg aaaatgataa
      301 aatgtatatc attccaccac actatcatgc tactgacgaa ctgtacaaag attatgaaaa
      361 aattaaaatc actgcatact cttcagatgc aacagaatta atgagtggac aggaatttat
      421 tagaatcgca aaagagaata atgtcgtgac ttggtaagga gttttatgat gaaatcagaa
      481 gtatatgtaa ttgcagaaat tggtgtaaac cataatggaa gtctagattt agctttagaa
      541 agtattgata aagctaaaga atgtggagct aacgcggtta aatttcaaac atttaaaacg
      601 aataagctag tttccaaaaa ggctccaatg gcaaaatatc agaaggataa tcttaaagag
      661 gacagttctc aatttgaaat gctaaaaaaa ttagaattat ctcaatctga ttttattaga
      721 ataaaagatt attgtataca aaataaaatt gattttttgt ctactccttt tgatgaagaa
      781 agtgcagact ttctaaaaca aattggtgta caaggattta aaattggctc aggagattta
      841 acaaatttac cttttcttag aaaaatagat atgtatgggt tgccagtttt attatctaca
      901 ggtatggctg atattcatga ggtcagtgag gctgtagatt gtttcataga tagtccagtt
      961 actattttac attgtacttc aaattatccg gctagttctg aagatattaa tttattggcg
     1021 attaacacat taaaagaaat gttcgggata cccgttgggt actccgatca ttcattggga
     1081 tatgatgttg gtatttgtgc tgttgcgatg ggtgctaagg taattgagaa acattttacc
     1141 ttggataagg aactacctgg tccagatcat aaagcttctt tagatcctca ggaatttgca
     1201 gattttgtaa agcatattag aaacactgaa atatttttag gtgatggtgt taagcgagca
     1261 atgccttctg aacttagtac aaaagaagta tcaagaaaaa gcctagtggt cactgagaat
     1321 ttaaatgtgg gagatattct tacagagaaa tctcttacaa taaaacgtcc tggaaatgga
     1381 atagctccta agtttttata tcaatatatt ggcaaaaaag ttaaaagacc aataagtgca
     1441 gatagtgttc tgcttgagga agatgttgaa tgaaaaaaat acttatagta agtggaacac
     1501 gagctgatta tggaatatat aaacctgttg ctgagaattt actacaagat aattgggatg
     1561 tatcatttgt tgtttctgga atgcatttat cccataaata tggatttact aaagaattga
     1621 tcttagctga cggatttaga attagtggag aagttcaaag tttgttttta gagaacaata
     1681 agggaaacat ggctcggtct gtggcattag agataaatgg tttcacaaat atatttgaac
     1741 aaactagccc tgattttgta ttattactag gggatagagg agaaatgttg gcagctgcga
     1801 tagcttgttt gtatcttgga atcaaaacag cacatattca cggaggcgaa gtgtctggta
     1861 ctgtagatga atcaataaga catgccataa cgaaaatagc atctgttcat tttgctgcta
     1921 cggaaaaaag caaagagcgt ttaataaaaa tgggggaaga ttcttggaga gtttttcccg
     1981 tcggagctcc tagaatagat actattttaa ataatagatt acctagtttt agagatataa
     2041 aagaaaaata tcatctaaaa tttgaaaaga aaaaatacgt tttgcaagta tttcatccag
     2101 ttgtaaccga actagaaaat atagaaaaac aaattttagc tttgatcaat tcaatggaac
     2161 aacaatctca accaattatt tgcattttac ctaatagtga tgctggtagc gaaattatca
     2221 gaaaaatgta ccagaaatat ttaaaaacgg atattattta tattacgaat ttagagccgg
     2281 aggaatacct gactatatta aaagagtgca aatttatgat cggaaattct tcttctggga
     2341 taattgaagc tgcatcattc aaaataccag taataaattt aggaaccaga caaaatggac
     2401 gagaacaatc taaaaatatt ataaatgcaa atctaaatga aaatgaaatt gaaactgctt
     2461 tgaataaaat acaagatcct cagtttataa aaagcttgaa aaatgttaag aacatctacg
     2521 gtgacggcaa cagttctaga agaataacaa aaacattgaa tcaaatattg aatgatgggg
     2581 aagatattaa gtggattcaa aaacaaatag cttattaaaa tatactagtg atccatacat
     2641 tattattggg gctggcggac acgcaaaagt cattattgaa accttaagat tagaaaataa
     2701 gttaatatat ggattttttg atgataagca aaataaaatg caagatctga aatatcttgg
     2761 gaaaataaac gaattgaata gctgggcgga tagcaaaaaa aaaatctata aatttattgt
     2821 agctattggt gactgtgtag taagaaaaaa gattataaaa aaattgaata tgccaagtga
     2881 gcaatatgga atagctgttc atccaagtgc tatagtggca aaagaaattt caattggtta
     2941 tggaactgtt atttttgcga atgctgtaat taacccagat gctgtaattg gagagcatgc
     3001 tataataaac acaggtagca ttgttgagca tgattgtaga attaataact atgttcattt
     3061 atctccaggt gtatgcttag cgggtggagt acatgtaggt gtaggaactc aaataggaat
     3121 tggttctcaa tgtatccaga tgaagaaaat tggatcttgg tgtatgatag gcgcagggag
     3181 cactattgtt aaggatattc cctcacattc cttagcttat ggcaatccag ctaaaattaa
     3241 gaaagaagga attaattttg aattctagaa ttttattatc aagtcctcat atgggaggaa
     3301 cagaagaaaa atatgttgct aaggctttta aagataattg gatagcgcca ttaggtgaaa
     3361 atgtggataa atttgaagaa agtattacaa aatatactgg ttctaagggc ggagcttctg
     3421 cgttaagttc aggtacagct gccatacatc ttgccttaag actgcttaat gttggaaaaa
     3481 acgatattgt ctttgctcct tcatttactt ttatagctac agtaaatcct attttatatc
     3541 aaggggctgt accagtgttc attgactcag aacctaatac atggaatatg agccctgttg
     3601 cactagagag cgcattagaa gaagctgaaa gaaacaacca attacctaag gcaatcatcg
     3661 tagtgaattt atatggtcaa atggctgatt atgaacgaat tttagctatt gcaaaccaat
     3721 ttaatgttcc agtgatagaa gatgcagcgg aatcgttagg ttctacttat aagggaattt
     3781 cgtcaggctt atttgggaca attgggatct attcatttaa cggtaataag atcatcacaa
     3841 cctcaggtgg aggagctcta gtgtctagtg ataaaaaata tgttgagcat tcaagatttt
     3901 tagcaacaca agcaagagat caagcaccgt attatcaaca tagcgaaata ggttataatt
     3961 accgtctaag caatatttct gccggtatcg gacgtggaca aatggaagta ttgaatgata
     4021 gagtagctaa acgaagagaa attttcaatc gatataagga agctttttct ggaattgata
     4081 gaattgaaat gatgccagaa gtggatggca tggtgccaaa tcgttggttg tctactttaa
     4141 cgatcaatga ttataattct attgatcaag tagttaacgt aataaataaa ctaaatgaaa
     4201 aaaacattga aacaagagct ttgtggaaac cactacattt gcaaccagta tttcaagggg
     4261 taaaaatgta ttaccatgat aataatctta gtgtgtcaga aaagttattt aatagcggct
     4321 tatgcttacc ttctggaagt aatatgacag atagtgaaca agaatttgtt attgatgagt
     4381 ttaaaaaaat attcaagtag gtgtgatatt ttatgaacat aggaattatt ggtttaggtt
     4441 ccatggggaa aagaagactt agactcattc aagaacactt taatgatatt ttactatgtg
     4501 gtgttgatac taatgaggcg agacgaaagg aagttgaaaa ggaatttgga ataccaactt
     4561 ataatagtct aacagaggca aaaaatttgt ttgaaataaa ttcagttttt gtttgtacat
     4621 cccctataac ccatgaattt attataaaag aggctttaaa tttagattgt aatatctt
//
LOCUS       DAYYAR010000172         4589 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000172 DAYYAR010000000
VERSION     DAYYAR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.175"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHAT0_002647"
     CDS             186..2855
                     /locus_tag="ACHAT0_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8452542.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACHAT0_002648"
     CDS             complement(2946..3560)
                     /locus_tag="ACHAT0_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8452543.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3679..4218
                     /locus_tag="ACHAT0_002649"
     CDS             3679..4218
                     /locus_tag="ACHAT0_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452544.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4215..4553
                     /locus_tag="ACHAT0_002650"
     CDS             4215..4553
                     /locus_tag="ACHAT0_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM8452545.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1419 a          790 c          860 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa ataacggtgt
     3601 acatttttaa aaggagatgt ataatcgaga tttagaaagc gttttcctca aaatttaatg
     3661 aattaggaag tgattactat gaaaaaatta tttagcgcac tttttattct gttatttggc
     3721 tttattggta ttgggacttc tggaaatata gttaaagcta gcacgatggt tgaacaaaca
     3781 agtgaaatgg caaaaacttt tagtcaatta acaccagaag aacaaagtta cttcaaatcc
     3841 aaaggattta gtgataaaga tactttttac actcaaaaga tagagcctca agatccgaaa
     3901 acacgttttt ctgcaaatgt attgcggtta accgcatcta ctaaaaaaat tactaatacg
     3961 caggggcgga cagaatatat tataacaagt cccaattcgg cgatgtatcg tatttctaca
     4021 cgcttgagtt taggtagtgc aaaatcatat actcaaacag taaatctagc taattcttat
     4081 ggttatggtg gaggaatgta ttttacattt actgggaaaa gacaatatta cagtgtaaaa
     4141 ttagctatgc aagtttataa tacatttggt gtagggtcag tctcttgcac tgcgggagga
     4201 ttgacaatag gaaaatgata atttcttact atgtcttaaa tagaagtgat gatgaaggac
     4261 tcttcgtttt tcaatcatta catgaggata cgatgctaaa agagttttat aatcggatta
     4321 agaataaaca gatagaaaca aatttatatt ttttaagtga ctctatcttg ccttttatta
     4381 gcaaccctaa aattcttaac ttgttgcaga caaaagattt gaaaagttta ccgattattg
     4441 tcattgatga ggaaattgta aaatatggcg cttatctaag cgtagaagaa ttagaaaaat
     4501 taactaacat tggaatgagt attcaggagg ataatggggt acatggaaca tagaatgaat
     4561 atgcacagtt gagaacggaa tttgacatt
//
LOCUS       DAYYAR010000173         4503 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000173 DAYYAR010000000
VERSION     DAYYAR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.176"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1259)
                     /locus_tag="ACHAT0_002651"
     CDS             complement(27..1259)
                     /locus_tag="ACHAT0_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8452546.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="ACHAT0_002652"
     CDS             1514..2083
                     /locus_tag="ACHAT0_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8452547.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="ACHAT0_002653"
     CDS             complement(2261..2701)
                     /locus_tag="ACHAT0_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8452548.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="ACHAT0_002654"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="ACHAT0_002654"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8452549.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..>4503
                     /locus_tag="ACHAT0_002655"
     CDS             3655..>4503
                     /locus_tag="ACHAT0_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8452550.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQ"
BASE COUNT         1343 a          843 c          898 g         1419 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caa
//
LOCUS       DAYYAR010000174         4434 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000174 DAYYAR010000000
VERSION     DAYYAR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.177"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACHAT0_002656"
     CDS             complement(543..2048)
                     /locus_tag="ACHAT0_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8452551.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACHAT0_002657"
     CDS             complement(2045..2731)
                     /locus_tag="ACHAT0_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8452552.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHAT0_002658"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHAT0_002658"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8452553.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAYYAR010000175         4418 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000175 DAYYAR010000000
VERSION     DAYYAR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.178"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..913
                     /locus_tag="ACHAT0_002659"
     CDS             59..913
                     /locus_tag="ACHAT0_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8452554.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACHAT0_002660"
     CDS             1010..3259
                     /locus_tag="ACHAT0_002660"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8452555.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4418
                     /locus_tag="ACHAT0_002661"
     CDS             3262..>4418
                     /locus_tag="ACHAT0_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8452556.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1556 a          625 c          807 g         1430 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagt
//
LOCUS       DAYYAR010000176         4367 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000176 DAYYAR010000000
VERSION     DAYYAR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.179"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..1363
                     /locus_tag="ACHAT0_002662"
     CDS             170..1363
                     /locus_tag="ACHAT0_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8452557.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(1529..1951)
                     /locus_tag="ACHAT0_002663"
     CDS             complement(1529..1951)
                     /locus_tag="ACHAT0_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452558.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            2322..2996
                     /locus_tag="ACHAT0_002664"
     CDS             2322..2996
                     /locus_tag="ACHAT0_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452559.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
     gene            complement(3158..4330)
                     /locus_tag="ACHAT0_002665"
     CDS             complement(3158..4330)
                     /locus_tag="ACHAT0_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8452560.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT         1299 a          798 c          817 g         1453 t
ORIGIN      
        1 ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa gctggattat
       61 ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt attctttttt
      121 ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa tgacaacgta
      181 tcagcgaaag ttattattat cgacatcggc gggaattgct ttagagaata tggatattat
      241 gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg gtacgcaagc
      301 gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt tttttggcat
      361 catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc tgttttcgat
      421 tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct ttcgctttat
      481 cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt ccgaaagttt
      541 ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc aggtcggtgc
      601 ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga agatgttgta
      661 tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct tgactgaacc
      721 aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca gactgtttga
      781 atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg tccaaattgc
      841 cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat tagggctttc
      901 ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt tggggatgat
      961 gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt tgtttttaat
     1021 ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga gtttggtagc
     1081 agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg gcgcagtgat
     1141 tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta tgaaccttgg
     1201 acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc attatagttt
     1261 aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg tcttgacaac
     1321 tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga gtctgtaaat
     1381 gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc caaatcaaaa
     1441 acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca gctttttcaa
     1501 ctgctaattt ccttctatct ggcggctact attctttgac gtccgtcatt tcaaagtact
     1561 ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga gcgtctgcat
     1621 aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga taattatatg
     1681 attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc ttttccatag
     1741 aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt tggaacaatt
     1801 cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat tcgttttgta
     1861 gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt ttaattttct
     1921 tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct actttactat
     1981 actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc aatagtgcat
     2041 aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat catttaatcg
     2101 tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt attattgcat
     2161 acttaataac agaactatag caaaaaatcc cgtgtatcaa aacgggatta gattaagttt
     2221 cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt gaaaaggaca
     2281 tttattgaaa acggttgttt tttacttaag gaggaaattt agtgaaaaaa ttagttgcat
     2341 tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct actactcaaa
     2401 gcacaagttc agctcaggct tccagcagca gtaccacgat ctccgaaagt acggcagatt
     2461 catcgaaaga acaagaacaa attgaaaaga aaaaagaaga agagaaacaa aaagcggaaa
     2521 aagctgcact agaaaaaaaa gtgacagaag cggaatctgc tttgtcacaa gctgaagcaa
     2581 atcctacaaa agaaaactat gcaactgctt cagcagctat ccaagcgcta cctgaaaaca
     2641 aacaagagtt gacaagccgt cttgctgcaa ttgattccac aatcaaagca aaagaagcag
     2701 aagaagaacg gaaaaagcaa gaagaaattg cacgacaaca agcagaacaa gcgcaacaag
     2761 aagcaatcgc acagcaacaa gcagaacagg cgcgacagca agcagcagct gaacaggcct
     2821 ctcagcaggc acaagccgca gctcctgagc aaaataatga acaaactgtc tatgtcacac
     2881 cgaccggttc aaaataccat acacataaat gtggaaatgg tacgtatacc cctgccactt
     2941 taagcgaagc tcaagctcgt ggtttaaccc cttgctccaa atgttatgga aactaattca
     3001 aaagttatac tataataata aaattcttct gaaacataaa aaaatagaag ctggcgttcc
     3061 agatagtaaa ccaaaaatag cagtttacac gtccatttcg tcagcttctc tcttttttca
     3121 tttagtctgt tccctctgct tttttggaca tcttcattca ttctttctca atcaaatagt
     3181 acacctcttt ttcttttcgc agtttatatc ttcctttttt caatttcatt gaaaactgtt
     3241 tcgaatgttt atgttttaca tggattttaa gcaggagctc ctcatgttgc aagcgatagt
     3301 tcctgccttt taaatcagaa aatctcacta gtccgtattc atcagaagct ttcacaaatg
     3361 gaactttttc tctagtaata tagtgtttgc cgttgagcgt ttttacagaa aagaccattc
     3421 cttcgattgg tcgtccgttt gtgttcttta cataaaaaga taaaggatag tttcgcttag
     3481 cgatggccaa agaatctaaa taagcgatga gtagccaaac taagccgatt gatccaagta
     3541 gtataccgat aaaccaaata aaaaatttcc attttttcca ttgatcaatt ccttggatcg
     3601 ctttttttgc ctcttttgct tgataaggaa tacgatgccc cgtcacaagc agtcgatgtg
     3661 tattgaccat ataaggagta caggtaagca aagtcacgag atctctgcct ttttggatac
     3721 ccaaagaatc tacttcatca ggtaagacca cttggatttt ttctacttga tacgccaatg
     3781 tctttccatt gatctggatg aaaaactggt ctcctttttt caacttaggc aaatctgtaa
     3841 ataattttgc ttctgggagt ccacgatgcc cagaaagaac ggcatgggtg tttttcccgc
     3901 cagtcggata agaactttct tccaataatg acgttccttt ttgcaaaaat atttccgttg
     3961 tctgatcgaa tataggtaag ctgacattga tttttgggat ttcgatgact ccaagcatat
     4021 gttccataaa aaaggcatta ctaggtaaat cttttaatgc ttttgcatca actgcttggt
     4081 tgaaactgcc tatccctggt gatacgtttt gctctgccaa ctgttggttt ttctttgtca
     4141 ttttttcctg ctgttttttt atttcctctg aattcttatt cgatgcttgc ttttgataat
     4201 ggatgatcat ttgctgggca agaaaatcat tcagtgaatc ttctacaaaa ggataagaaa
     4261 agatcccgat cccaacgact aaaatgattg ccatgaagat gtccagtatt cttattctgc
     4321 ttttattcat catgtccacc tattttctaa tatgtatatg aagaggt
//
LOCUS       DAYYAR010000177         4335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000177 DAYYAR010000000
VERSION     DAYYAR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.180"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHAT0_002666"
     CDS             127..579
                     /locus_tag="ACHAT0_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452561.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHAT0_002667"
     CDS             593..1282
                     /locus_tag="ACHAT0_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452562.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHAT0_002668"
     CDS             1407..3758
                     /locus_tag="ACHAT0_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8452563.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHAT0_002669"
     CDS             3838..4233
                     /locus_tag="ACHAT0_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452564.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1394 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatt
//
LOCUS       DAYYAR010000178         4312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000178 DAYYAR010000000
VERSION     DAYYAR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.181"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..380
                     /locus_tag="ACHAT0_002670"
                     /pseudo
     CDS             31..380
                     /locus_tag="ACHAT0_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(652..942)
                     /locus_tag="ACHAT0_002671"
     CDS             complement(652..942)
                     /locus_tag="ACHAT0_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452565.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1344..1580
                     /locus_tag="ACHAT0_002672"
     CDS             1344..1580
                     /locus_tag="ACHAT0_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452566.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1587..3539
                     /locus_tag="ACHAT0_002673"
     CDS             1587..3539
                     /locus_tag="ACHAT0_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8452567.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3611..4108)
                     /gene="dfrG"
                     /locus_tag="ACHAT0_002674"
     CDS             complement(3611..4108)
                     /gene="dfrG"
                     /locus_tag="ACHAT0_002674"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8452568.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1472 a          751 c          723 g         1366 t
ORIGIN      
        1 taacttaaaa gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa
       61 atctacgata gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg
      121 acctatggat tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata
      181 ttgcttttaa aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag
      241 gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt
      301 acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat
      361 cctacaattc cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc
      421 cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc
      481 ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata
      541 tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc
      601 catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat
      661 tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat
      721 tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat tattataata
      781 acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga
      841 agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg
      901 caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc
      961 attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt
     1021 tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact
     1081 aaaaaaaaga ggcagcagta tagatttaag tagttcagtt ttggagtaca aaaagtgaaa
     1141 atgtaaacct aaatgagtat caaattgcaa ctgaatttga tactcgtttt ttattgttaa
     1201 atcaacgttt gttgttaatt tacaccacaa aatgtaagtt aaaatgtata taaaaatgcg
     1261 aacgtgaaat aattattgta ggaatgcaaa ttggtgtgtt aaaatggtta caatttacta
     1321 agcaaaaagg ggtgcatttt cctatgcaat ttcttgagcc aaaaaataag aacgctaagt
     1381 ctgtggattg ggaaatatca gagcaagtaa gggtgatagt taagcaatac gctgaatatg
     1441 cagagcgtac tgaaagcgaa gcagtggatg aattcttatt gaacatttta gacgataaga
     1501 agtttattga atggattgcc aacaagcgaa gtaataagcg gattgttgaa aaaatgggta
     1561 tcaaagatag agtgggttga tagacaatga ccaagcttaa aagattagcc acgaaggaag
     1621 atgaaattgt agatgttaaa attccgattt caaacgaaga attaaaagat agggctaaac
     1681 aatatgattt attaactcca aaacgatttg caacaaggta taacaaaatg ttgttcttgc
     1741 caacgtcttt taaatggaac ggttctgaat atcccattca atataattac tgcatcaatc
     1801 cgttttgttg taattttggg aaagaacagc acaaattcaa agatgtaaaa gggaaaccaa
     1861 gtcgatataa aatgactggt agttccaaag ataaaggtca taaaggtatg tactgcaatg
     1921 acaatcctat tggcagaggt gtatcgcaaa actgtacagt aaccccttta tctaattggt
     1981 ctgttgttga agaaattaaa cgccttatag aaatcaatag catccaagat gttgagccag
     2041 attatcaatt tcataaagaa ggctgctcgg aagaagaatc aaccccattc aatgagccga
     2101 agcaatttta caaaaggggg aaaagtaggg gcaaatctca acgctatcaa tgtaaggcgt
     2161 gtaagaagtt cactaatgtt ctaccaaaaa gagaagaaac cactacttat caccaacaaa
     2221 agaacaccat tcttccaatg ttcgctaaga tggttgttgg aagggtttca gtcagtcgta
     2281 cttgtgatat tttaggaatt ggagttggaa cctactatca taagctggag tggctgtata
     2341 ggcgttgttt ggagttttta gaacgatatg agacacaacc ccttcaaaca aagaaattta
     2401 acgagatgtg gcttaatacg gacaagatgc actactacct aaataacgtc cgtaagaaag
     2461 gacaaggttc aaagaaatat acggggtttg aagaccttaa tatgcaaacg tatatagttg
     2521 tatctgctga ggtcttatca agatatgtgt tccgttctga tgttgcctat gattggaata
     2581 tttcaatgga tgaattaaat gaagatacaa gaaaattcaa agaagaccat ctcaatactt
     2641 ttagtcgtaa aaatgatagg ttagattggt cttattatcc acaagaacca tccgctaacg
     2701 atagcgaaaa taggaatgct tatcttcacg aattagggaa aattactaat aggagtagat
     2761 ttgtcgatgg tttaaatgtt gatgctcctt atactacaac tgctcactat tggttaatta
     2821 aacaaatggt taatgccgac gaatggcgaa tgattagtga tgatgacttc tctattagaa
     2881 acgcctttta tagagtattc acgaaagaat taaggttgag cgatgcacat cattttattt
     2941 gccaagttaa caagacgaaa agcagaaaac aatgcttaaa agaattcgga caagcaaaag
     3001 cagaactgct cgattgggga gatatcagag gattcaaaac aaagtttcta cggactttgg
     3061 catctcatta tctaactgaa ttactaacaa gtcatcaatt ccacgaagag gctattagta
     3121 aagatggaga aagatatcgc aaatatgccg ataaccctat caaacatcca ttagccacta
     3181 aggataaagg attttattca gttgattgta gaacggattt atctgctctg gaaccaaacg
     3241 aaatcgcaaa gatgctatta aatgttaatg accattcgac caatagcttt attcaacaga
     3301 taagacgtta tatatcatct cttgaaagac cattaacaac agctcgtggt gataagaaga
     3361 gctatattta cgctaacttc aatccgaagt atgctcaatt tgcgattaca attctaagaa
     3421 cttactacaa cttttgtcgc ccttttaaat cagcggataa aaaggttttg acacctgctc
     3481 aaagattagg gattacagat aaacaatttg attggaaaga tataatttat ttcaaatgac
     3541 aaaaagagct tggagtaatc ccaagctctt gtgctttatc tttactgttc ttcttcaatt
     3601 aacgcacccg ttaactcaat aagttttttc tttcatatac atgaaaatag tagttatacg
     3661 gatttttatc attctttatc cctttttggg caaatacctc attccattcc tcataattca
     3721 cttctggaaa aaaagtatct ccttcgaatt catgatgtat ttttgtgatg tacattttct
     3781 caacataagg gaaaaacaaa ttataaatct gttctcctcc gaaaataaaa atttcttctt
     3841 cgtttttaca taactcaaaa acatcttcta ttgaatgaac aatttcacaa ccattaaagg
     3901 taaacccctt atctctcgtc agaataatat ttcttctgtc aggtaaggct cttccgattg
     3961 attcaaggtt cttcctacct aatattatcg gatgtccctt tgtagtattt ttaacatatt
     4021 cccagtcctt gggaatcctc caaggaatgt cattctcttt gccaatcact ctattcttat
     4081 ccatcgcagc aatcaaagaa actttcattt ataacacagc ctttttaact aatttaccat
     4141 ctaatttctt atttctatca aaaaaccttt tctcattatc ctgcccgtta gttgaataag
     4201 caattttttc tataagttat attatcataa taccacaaaa tcacaaattt tcattcaatt
     4261 atcattttca attgcatttt ataaatcatt ttgtatgtaa tttggtatgt aa
//
LOCUS       DAYYAR010000179         4099 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000179 DAYYAR010000000
VERSION     DAYYAR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.182"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..1239
                     /locus_tag="ACHAT0_002675"
     CDS             46..1239
                     /locus_tag="ACHAT0_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8452569.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            1232..2959
                     /locus_tag="ACHAT0_002676"
     CDS             1232..2959
                     /locus_tag="ACHAT0_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8452570.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            3353..3550
                     /locus_tag="ACHAT0_002677"
     CDS             3353..3550
                     /locus_tag="ACHAT0_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8452571.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            3552..3863
                     /locus_tag="ACHAT0_002678"
     CDS             3552..3863
                     /locus_tag="ACHAT0_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8452572.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            3967..>4099
                     /locus_tag="ACHAT0_002679"
     CDS             3967..>4099
                     /locus_tag="ACHAT0_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFM8452573.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     "
BASE COUNT         1436 a          634 c          883 g         1146 t
ORIGIN      
        1 tttttcagaa agaaaaatga atttacataa aaaaggagaa tttgtttggt aaaagtagaa
       61 gtaaaacatt taacaaagat atttggtaaa aagacacaag ccgctttaga tatgatgaat
      121 gatcaccagc cgaaaacgga gattttaaag aaaaccggtg caacggttgg tgtgtatgac
      181 gtgaattttg acgtaaaaga aggagaaatc ttcgtgatca tggggctttc cgggagtggg
      241 aaatccacat tgatccgtct attgaatcgt ctgattgagc caacttcggg cagtatctat
      301 attgatggag aagatgtatc gaagttaagc aaagaagaat tgcgagaagt ccgtcgtcat
      361 aaaatcaata tggttttcca aaacttcggt ctgtttccac atcgaacgat cttggaaaat
      421 acagaatacg ggttagaagt ccgtggtgta cctaaagaag aacgacaaga aaaagcagaa
      481 aaagcattag aaaattctag tttgctttca tttaaagacc aatatcctag tcagttatct
      541 ggtgggatgc aacaacgggt tggcctagcc agagctttag caaatgatcc tgagatcttg
      601 ctgatggacg aagcgttctc ggctcttgat ccgctgatcc ggcgagaaat gcaagacgaa
      661 ttactggacc tgcaagccaa tgttcaaaaa acgattattt tcattaccca tgatttgaat
      721 gaagctttac ggattgggga cagaatcgcc ttgatgaaag acggcgaaat catgcagatc
      781 ggaacaggtg aagaaatatt gaccaatcca gcaaatgatt atgtacgtga atttgtggaa
      841 gaagtcgatc gttccaaagt gcttacggct caaaacatca tggtacctgc attgacgacg
      901 aatatcgaat ctgatggacc aaacgttgca ttgacaagga tgcgtaatga agaagtcagc
      961 atgctgatgg ctgttgacag aaagagacac ctgaaaggga tcatcactgc agatcaagcg
     1021 ttagaagcta ggaagcaaaa acgtccattg atcgacttcc tagatgaaaa tgtgacggtg
     1081 attgggaaag acatggtggt aagtgatatt ttcaatatta tttatgactc accgactcca
     1141 cttgcagttg tagaggatgg aaaattaaaa ggtgtcgtca tccgaggtag tgtgattgaa
     1201 gcattagcag aaacaagcga ggtgagtgaa catgaataat ctattgaact atcaattacc
     1261 agtggcaaac tgggtagaaa atatcacaga atggtttacg acaacttttt ctggcctatt
     1321 ttcattttta caaacgattg gacaggcagt gatgtcgggg attacaaatt tactgttagt
     1381 gattccagct cctttgttta tcttgttatt gacggtagca gctttcttta tttctaagaa
     1441 acgtccagga cttacgttgt tcacattaat cggtctatgg tttatttata accaaggtct
     1501 atggaatgat ttgatgaata ctgtcacact tgttctttta tctagtgtga tctcgatcat
     1561 cattggggta ccattaggga tcttgatggc aaaaagcagt aaagcccaaa gtattatcaa
     1621 accgatcctt gactttatgc agacaatgcc aggattcgtt tatttgattc cagcagttgc
     1681 atttttcggt atcgggatgg ttccaggggt atttgcctca gtcatctttg ctctgccacc
     1741 aaccgttcgt tttacgaatt tagggattcg acaagttcct aaagaattag tagaagcttc
     1801 tgattcattc gggagcacaa gtcgtcaaaa actattcaaa ctagaattgc ctttagctaa
     1861 aagtacgatc atggcaggga tcaatcaaac aacaatgctg tcactttcaa tggtcgttat
     1921 tgcctctatg atcggtgcac ccggcttagg acgcggcgta ttatcagcat tgcaaagagc
     1981 acaagtcggg aatggatttg tcaacggggt tgcattagtt atcttagcaa tcatcattga
     2041 ccgttttaca cagcatttga accagccaaa caataaaaaa gctgcaggag caacaacaca
     2101 gaaaagtaaa aaacgtcaag gactgatcat tggtgctgta gtagtagtga tcttaggtgc
     2161 gattggtata gggagttttt catctgccaa agaaacaaaa cgtatcaact tatcttatgt
     2221 tgaatgggat acagaagttg cttcaacaaa tgtagtcggt gaagtattga aacaaatggg
     2281 ctatgatgta acgatgacgc cattagataa ttcaatcatg tggaaatctg tttcaaatgg
     2341 cgagtcagat gccatggttt cagcttggct acctaaaact catggttcac aatatgcaca
     2401 atataaagat caagtcgaag acctaggtgc caatttgaca ggagcaaaag ttggtttagc
     2461 agtgccagcc tacatggatg taaattcgat cgatgagtta accgatcagg caggtaagaa
     2521 aatcatagga atcgagccag gagctggtgt agttacggct gctgaaaaca cgatccaaaa
     2581 atatgacaac ttgaaagact ggaaagtaga gacatcttct tcaggagcaa tgaccgttgc
     2641 actaggtcaa gcgatcaaaa aacacgaacc aattgtagtt acaggatgga cacctcattg
     2701 gatgtttgcc aaatatgatc tgaaatactt agaagatcct gaaaacggaa tgggaagcga
     2761 agagcagatc catacgatgg ttcgaaaagg attgaaagaa gaccagccag aagcttacaa
     2821 agtattagac aatttccatt ggtcagaaaa agatatggaa aaagttatgt tggaaatcaa
     2881 caatggaaaa gatccacagc aagcagcaaa agactggatc aaagagaacc aagaattagt
     2941 cgaaagctgg aaaaaataaa aagacagaca cgaaataaat ggagcttcca aaattggatc
     3001 aaatatccaa atttggaagc tccatgttcg tctctatctt ttttgtctat aaaaaaagag
     3061 aatgcgtagc tcatcttcga aacgtccaaa gacgccgaca aactattatg aatttatggg
     3121 tgcgagtcaa agagatcgat cgtaggtatg aaagactaat gtgattaaag tttattatgt
     3181 acgaagtgca ttctcaaaaa atattaaaga atatacatag tttatatact taaaagtgat
     3241 taaattctta ttcaggaatt attttgctac gtttcaagaa ggaattgttt tttgaaattt
     3301 aatgaatgtt agaataatag tctaaaaaca ttaatttagg ggtgattaga ttatgaaaca
     3361 tttaaaaatt ttgtctatta aagagacaca acttatctat gggggtacca ctcatagtgg
     3421 aaaatattat ggaaatggag tgtattgcac taaaaataaa tgtacggtcg attgggccaa
     3481 ggcaactact tgtattgcag gaatgtctat aggtggtttt ttaggtggag caattccagg
     3541 gaagtgctaa aatgaaaaaa aatgctaagc aaattgttca tgaattatat aatgatatat
     3601 ctataagtaa agatcctaaa tattctgata ttcttgaggt tttacaaaag gtatatttaa
     3661 aattagaaaa acaaaaatat gaattagatc ccggtccttt aataaataga ttggtgaatt
     3721 atctatattt tactgcttat actaataaaa taagattcac tgaatatcaa gaggaattaa
     3781 taagaaattt gagtgaaatt ggaagaactg ctggaataaa tggtttatat cgagcagatt
     3841 atggagataa atctcaattt taatgatact tataatatta aatatgtctc aaaatttttt
     3901 gattagaaaa ataggtatat tgaatcagtg acataaaagt atttatagtt tgtaggggga
     3961 atcgtaatgg aagaaaaaaa tagactaaat gctaaacaat gttcagatca agagcttaaa
     4021 aaaattaaag gaggtgctgg aacaaaacct caaggaaaac cggcatcaaa tttagtggaa
     4081 tgtgtttttt cattattta
//
LOCUS       DAYYAR010000180         4031 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000180 DAYYAR010000000
VERSION     DAYYAR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.183"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..638
                     /locus_tag="ACHAT0_002680"
     CDS             330..638
                     /locus_tag="ACHAT0_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8452574.1"
                     /translation="MFQNKGQRYVTKGVMDSLLVELQTLCWNLIDQNVQKKLPLDYLQ
                     IFEFSIEKGNQKLVHRQEEPEARKEYLISPKLRLESVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            665..964
                     /locus_tag="ACHAT0_002681"
     CDS             665..964
                     /locus_tag="ACHAT0_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8452575.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGTLPKNVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVCGLLVEGRLVWSQIGGTNE"
     gene            1138..1683
                     /locus_tag="ACHAT0_002682"
     CDS             1138..1683
                     /locus_tag="ACHAT0_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8452576.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEEKEKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFSRGNDEQSLE
                     ILTTIFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLSDHSLLFQ"
     gene            1839..2135
                     /locus_tag="ACHAT0_002683"
     CDS             1839..2135
                     /locus_tag="ACHAT0_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452577.1"
                     /translation="MKVDGECLTSSYKSIYEFFEEKGLDTDLLYQFYIAFRGQQLSFP
                     MKMYDRELVAQKIETMIQENHSIDSRKLTDRYGFSTRWIQSIIRQQQKEQHVHG"
     gene            2128..3018
                     /locus_tag="ACHAT0_002684"
     CDS             2128..3018
                     /locus_tag="ACHAT0_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HFM8452578.1"
                     /translation="MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGNVSDIEGLI
                     EKGPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYS
                     LRLAQEISYIYGFKDLMGETGEFSEEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQ
                     ALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLS
                     MKPMATKLQTELGKNVAYTEKNLQQDLDILEGEFEEVSSDIVSSDTVLSQLERLSHLL
                     ETNMITEEEFQQLKQELLKK"
     gene            3100..>4031
                     /locus_tag="ACHAT0_002685"
     CDS             3100..>4031
                     /locus_tag="ACHAT0_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8452579.1"
                     /translation="MKAKRLFLLGTILLLGGCSTVSQNEQKKQVDSTTYETETTRKES
                     STKEIETTTQETKTTESSSKATSSDTSDFQIKQELWDTNKASQLATFVPQWGKTLGQE
                     YKSYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGNAGFNLVAVYSDV
                     ETGEYLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFTDIINGQT
                     PQTPVVEESTQQATTTTTQSTSSSGISNWEAAKETVLNNKALWIYESPDKNEANTNII
                     ESTQYRKDLMQDENGEFYPVDISTDDIGNTVMNGG"
BASE COUNT         1495 a          580 c          780 g         1176 t
ORIGIN      
        1 ttttgacttc ctttcttgta ttatttataa taacaatagt agtataacat taataggata
       61 gttcattaag caaaattaag tatcttagtt actaataact atataaattt taaacttgta
      121 gtcgcaattt agtatagagg tactaaatat gaaaactcca tacttttaga aaagcatttc
      181 ttatatttag ttgtgatgtc tattcagtta attgaaataa ataatatagc aatgaaatag
      241 agataaattc aaatatatat tatatccatg aagtccaatc ttctttatag aagattgggc
      301 tattttttac gacaaaggag ggaaaagaaa tgtttcaaaa taaaggacaa cgttatgtca
      361 caaagggagt aatggacagt ttactggtgg aattacaaac tttatgttgg aatttaatcg
      421 atcaaaacgt tcaaaagaag cttcctttag actatcttca aatctttgaa ttttcgatag
      481 aaaaaggaaa tcaaaagttg gttcatcgtc aagaagaacc ggaagcaaga aaagagtatc
      541 tgatttcacc taagcttcgt ttggaatcag tgtctcaaaa aatatgggtc attgattcgg
      601 gtgagtatca aacgatgtta ttaccagaag aatactgata tctaaaagaa aggagcgaaa
      661 tgacttgtta gaaacggcta tcgaagtact ggaaaagtgt gcacagctag tcactactcc
      721 tgaagagtgg ggctatgaat ccgttacgat ggaaaaagag gaaattgaaa tgggtacgct
      781 tcctaaaaat gttcatctac cacgtttagt gatgacacat ctttatattt attgcgcacc
      841 agaagatgga aaagactatg tagtttattt cattacagat attaccagcc agcgagaatt
      901 tgtttgtggg ttactggtag aaggacgatt ggtttggagt cagattggag gaacgaatga
      961 ataagatcga ttatttgaga atgattgcga aatgtttaga agatatcctt accgcgattt
     1021 atcgaattct gaagaaaaaa ggagaaagaa tatgaaatat tttcaactag tattgacact
     1081 tataacggca atggtaattg aattgcttga acaaaaatta gcataggaga aaaagagatg
     1141 attgaaaaag aattagtgac cacacaaaca gaagtttggc gagatcctca tcatacccat
     1201 cgctatgttt tcaaacgaca atgggcaatc aaaggaaaag aagagaaaga aaaattagca
     1261 gtagtcatta ccattcgtcc aacagataca gaaccattta cgaatgattt gagtatgtta
     1321 ttaatcgaaa agaatatccg acaattggga tttactggtt ttattgccgt gaatcttttt
     1381 tcgtatacaa aagcgaaaag tcctcgggta ttttctcgtg gcaatgatga acaatctttg
     1441 gaaatattaa cgaccatttt tacagaaaag aaaatcaaca ctattatttt tgcttgtggt
     1501 tcgattacgg caacgagtca agtggcttat gaacaagcaa agaccattta cgatcaattg
     1561 agtgcgaaac aaaagaagat gaccaaagta ttgataaatg cagaaggcaa gttatctcac
     1621 ccattaagta ttcatgttcg taatgaatgg caattgtcgg atcattcttt gttatttcaa
     1681 taaacgaaaa taagtattca atgtatatgt ttctttttct gttattttac ttgttctttt
     1741 gcagactaaa caacttgtaa aacctcttgt tatatgcatt atcaatgaag gaatcaatat
     1801 gatattttta aaatgtcagg ggaaatggag gtgagcaagt gaaagtagat ggggaatgtt
     1861 tgacgagttc gtataaaagt atctatgaat tttttgagga aaaaggacta gacacggatt
     1921 tactttatca gttttatatc gcttttcgtg ggcaacagtt gtcgtttcca atgaaaatgt
     1981 atgaccgaga attagtcgct caaaaaattg aaacaatgat acaagaaaac cattccattg
     2041 attctaggaa attaacggat cggtatggtt tttcaacgag atggattcaa agtattatca
     2101 gacaacaaca aaaggagcaa catgtacatg gctaatgaag aaacagtagg tttgagaatc
     2161 atagaagaag cattcaaatt accaatggtc aaagttgatc gtcgagcatt tttgattcaa
     2221 acatttcaag ggaatgtttc tgatattgaa ggactcatag aaaagggtcc acaagcattt
     2281 ttatccaaag aaatgctaga tcgtgaagca caaaaatgta ttcaatcgat taccttgaaa
     2341 tcaagtgcgg tatcgttagc aactggactg ccaggaggta tcgctatggc agcaactatt
     2401 ccagcggatc tccttcagtt ctttggttat tcattacgat tagcacagga aatttcgtat
     2461 atttatggat ttaaggattt aatgggagag actggagagt tcagtgaaga agcaaagaat
     2521 acgctgatat tgtatttggg aattatgctt ggagtaactt ctgcaggttc tgccgtacga
     2581 gcgatgtctg gaaagctgtc tacacaagca ttgaaaaaga ttcctcaaaa agcattgaca
     2641 caaacgctgt attatccagt aatcaaacga gtactaggta tttttggtac aaagttaact
     2701 aaaaatacct ttgcgaaagg tatttctaaa gcagtaccag ttgtgggagg cgttgtttca
     2761 ggaggcatca attacttgtc tatgaaacca atggcaacga agcttcaaac agagttaggg
     2821 aaaaatgtag cgtatacaga aaaaaatctc caacaagatt tagatatttt agaaggtgaa
     2881 tttgaagaag tatcttctga tatcgtgtct tctgatacag ttctttctca attagaaaga
     2941 ctttctcact tgttggaaac gaatatgatt acagaagaag agtttcaaca attgaagcaa
     3001 gagctgttaa agaaataatt atttagaaaa agcaaccact tatacgggtg gttctttttc
     3061 ttgaatgaat tcaaatttat tattaggaga agaaaaagga tgaaagcaaa acgattattc
     3121 ctattaggca cgattctttt acttggagga tgttctactg tctctcaaaa tgaacaaaag
     3181 aaacaagtgg attcaacaac ttatgaaact gaaacaacaa gaaaggaatc ttctacaaaa
     3241 gaaatagaaa caactactca agaaactaaa acaacagagt cttctagtaa ggcaacgagt
     3301 tcagatacga gtgattttca aataaaacaa gaattatggg atacaaataa agcatctcaa
     3361 ttagcaactt ttgtcccaca atggggaaag acattaggtc aagaatataa aagctacaat
     3421 cctcagaaca atgtaagtct gtatggtacg cctttaccaa gtgcagttat tgatggaaat
     3481 tggaaaatgg caatcaatga agcaccagtt actgtacaat ggtcagaaga tggaactgga
     3541 aatgcaggat ttaatttagt agcagtttat tctgatgtag aaactggaga atatttaggg
     3601 cagcatgttt actttttcgg ttttcaaaat ggacaaccta aagtttattt aacgcaacaa
     3661 aatcaaggta atgaaaataa ttatctgtac tttaacgaaa ctcaaaatca acagctaaaa
     3721 caaggattta cagatatcat taatggacaa actccccaaa caccagtagt agaagaatct
     3781 actcaacaag caacaactac aacgacacaa tctacaagta gttcaggaat ttcaaattgg
     3841 gaagctgcta aagaaactgt actaaataat aaagcgttat ggatttatga gtctccagat
     3901 aaaaatgaag caaatactaa tattattgaa tccacccaat atagaaaaga tttaatgcaa
     3961 gatgaaaatg gagaatttta tccagttgat atttctactg atgatattgg aaatacggta
     4021 atgaatggag g
//
LOCUS       DAYYAR010000181         4022 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000181 DAYYAR010000000
VERSION     DAYYAR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.184"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(72..614)
                     /locus_tag="ACHAT0_002686"
     CDS             complement(72..614)
                     /locus_tag="ACHAT0_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8452580.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            749..1138
                     /locus_tag="ACHAT0_002687"
     CDS             749..1138
                     /locus_tag="ACHAT0_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452581.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1142..1906
                     /locus_tag="ACHAT0_002688"
     CDS             1142..1906
                     /locus_tag="ACHAT0_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8452582.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2035..2982
                     /locus_tag="ACHAT0_002689"
     CDS             2035..2982
                     /locus_tag="ACHAT0_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8452583.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3112..3852
                     /locus_tag="ACHAT0_002690"
     CDS             3112..3852
                     /locus_tag="ACHAT0_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452584.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1344 a          683 c          810 g         1185 t
ORIGIN      
        1 aaaataataa ataaaaactg tctaacattt aaaatttaat ttagatatta gacagttttt
       61 aagtttgtga gttattttcc ttgtcgtttt ctcacttttt tagtccagaa accacctgaa
      121 atatattttg gctcataaga aatgataaaa gcacgctctt cgatttcttt gatcaggcga
      181 tacagttcac gttcgctttt tctaggagaa agtatttcga gaactagccg ctcaccttcc
      241 agccccattg cagaagtctg tgtcacacca taaccgtgtt ctctcagtat acccggcaaa
      301 cttttatctt ctgcgctatt tgctggcaaa atcgttgtga ccatgatgta tcctaatgcc
      361 aatttgtctt caatcaaaat tccgacactg atacctactg cgtaacctaa cgcatagaca
      421 aataaattca gtggattatc taaacgatct aaaacaaggg ataagccgac gatataaatc
      481 gtgatctcga ccatactgac aagtggagca atcagtcgat agcctttcat tgtcaacatg
      541 aaacgtaacg tgttcagagt gatataagca aaattaatga ggaagatcat ccccaacatt
      601 tttggatcaa tcatttgtaa aactcctttc atttatataa caacaatatc aaaaaaaatc
      661 gtaagtgaag aaaaattgaa actttataca tacaaaaaaa tgataaaatg attataatag
      721 aattataaag ataggttagg tgattaaaat gaaaacggtc aatcgtgtgt tttgtgccag
      781 ccacgtgcta agtctccttc tcagtatata cttaggtgtc tattcgattc aacagccaat
      841 tgaggtagag aacattgttt ttcagatgtc gctgacagat ggtttaattt taagcgtttt
      901 tttctttttg atactgttta ttggtaatat tttcggtagc gttgccagtt ttttgaattt
      961 ttctatttat ccactccttt cgctagcatt aggagtggtg ggactttgtt ctctgtgctt
     1021 atttcccgag ccattcagtc ctgcatttat attatttggt acactgaacc tgttccagac
     1081 aactgttgga gcatggttgc tatggcgatc aggaaattta atgaaaatag gtgagtaaca
     1141 attgaatcca attatattga aacgttctgt ccatcatatt ccagtgttag aagttgttct
     1201 gaaagaatta agagaggaac gtattcccgt tgtcatctat tatcacggat ggcagacaag
     1261 taaggagtta gttttgacgc aaggaagaaa aattgcgcag cagggcattc gtgtgatcct
     1321 gccagatgcg atgaatcatg gggagcgcaa acagccggtt tcttctattc catcctatac
     1381 attttgggat agtatctacg gaaatctgtt tgaatttgac acacttatca agcatttaga
     1441 aaaaagaggt cttctacatg atcagcttgc agttggcggg gtttcgatgg ggggaatgac
     1501 aacatgcgcc ttgttggcta aacatcctca tatagtagcg ggcgtgtgtt tgatgggttc
     1561 ccctgcacct ttggaatatg gaaaagaaat cgtaaggcgc gcatcggaat ataattatgt
     1621 cgtaccaaaa gactattttg atttgatttc atgggttaag agctatgatt tgtcattgca
     1681 gccggaaaag ttagctggac gtcctctgtt cttttggcat ggacaagctg acgaaaaaat
     1741 cccttatata caaacggaac gatttgtaga aaaaaatcgg cataagtctt acggaaaaga
     1801 aattgttttt cgttcctcaa aaacacagcg acatatggta caaataccac taatggaaga
     1861 agcttccact tttttagcaa aaaatttgat aaaaaagtat aaataattga aacggaaaat
     1921 cactcatgac gatagaggat tttccgtttt ttattttggg ctataataaa ctctgttagg
     1981 aaaatttggt ataattgata gtaagaatga caacatgtta gaaaggaaga gagcatgccc
     2041 actttagcag atgttgcaaa acgtgccaat gtttcaaaaa tgacggtctc tcgagtcatc
     2101 aatcatccgg aacaggtgac tgacgaattg aaagagttag ttttctcagc aatggctgaa
     2161 ctggattacc gtccaaatat tgctgcaaaa gcacttgtca cgaatcggtc gcagatcatc
     2221 aaattattta tactggaaga aatcgacaca actgagccct attacatgaa tttgctgatg
     2281 ggaatcgcta aacggatcgg taaagcgcat tattctctcc aattagttac aaatgatgcc
     2341 ttcgacgtcg gttcttgtga tggatacatc atcacaggtg tcagaaaaaa agacttcgat
     2401 tggatcgacc gtttggaaaa accagttgtc ttgtttgggg aaaattctga aggatatgat
     2461 tatgtagaca gcgataatga atatggtaca gcaaaagcaa cgcaatatgc cttagaaaga
     2521 gattatcaaa cgattattta tatcggtatc gaagtggacg agccttttga aatttcaagg
     2581 gaaactggtt atttatctgt gatgaaggaa gttgatcttc ctagtaaaat cttgcggttt
     2641 ccaaatcatt cgacagaagt agaaagatat atgacagaaa actgggcaga ttttacagat
     2701 aaaacttgtt ttgtatgtag ttcagatcgg ctagcactag gagcagtaag agggattata
     2761 aatgctggag gacacttgcc cgaagaattt ggtgtgattg gatttgatgg ggtattttta
     2821 gaccaaatca gttcacctaa attgacaacg atcaagcaag atattatacg tatgggtgaa
     2881 gtatgtggag aaatgttgtt aaaaaaaata gaagaaaaag gggcgagtca aggttatcgt
     2941 cgtttttttc ctgaactagt tatcagagag acaacgaatt aagtcttatt actatatata
     3001 tatttatgaa attcatcgct attttcttaa aataatcagc gcattgctac tgaaaacaat
     3061 gaatctgtgc tattattgag aaaagttcca tttttgagaa ggatggtttg aatgaaagaa
     3121 atcagacgaa aacatttccg ttttcctgcg tacgatgatg agttaggcgt aaaattaacc
     3181 aacgacaaga agcgagtatt atttgacgga caagaagatc ttttgactga acagaatcag
     3241 tccccgtttg aacagatgaa agaatttcat ttcacgaaca cgaacaccga gaaaatccgt
     3301 cgcaagcgtc gtgcaacaaa tggattgaag tcagctccgc gtaaggaaaa tttggaaaag
     3361 cataaaggaa aattaccgga ttataccggc cattaccaat cagaaatcac atcgacagga
     3421 cgaaaaaaac tttttggtga ttcctctcgt tctgtcagac aaagtgaaac aaggacagag
     3481 tccaagcata ctacacgaaa cgcatctgtt gacacgaagg tgcgggagcg ttcttatttt
     3541 gttcctaaat atgtacccgc atcgattatc ccagacccta gagaaagcaa aatttctgag
     3601 gatcatttaa tcgaatcgat gaaaaaaacg gatgactctt atctgttgtt cgatacagaa
     3661 ccgacgccgt atcaagaaaa aagaaatggt gagccgtctg tacgaacatt caatcgaaca
     3721 aaaacgttag aggaaacaaa gcagtcagaa gaaaaaaaat cgaaaaaaag ccatggtatc
     3781 ttagaacgct cattacaagg attgatcgat gatcaaggaa actctttggg agaaaatagc
     3841 tattttaaat aagtgatata caaatttata ttcggctaca gttggaaagg aaacgctgcg
     3901 gcgtttcctt attttatact ctatccgaaa gtttatatcg tatcctcaaa tttttttgag
     3961 tttaagcgaa ttttttctcc caataagtaa gaaaataatg gcaaaataaa aaaacaaact
     4021 at
//
LOCUS       DAYYAR010000182         4016 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000182 DAYYAR010000000
VERSION     DAYYAR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.185"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(175..1461)
                     /locus_tag="ACHAT0_002691"
     CDS             complement(175..1461)
                     /locus_tag="ACHAT0_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8452585.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1454..2722)
                     /locus_tag="ACHAT0_002692"
     CDS             complement(1454..2722)
                     /locus_tag="ACHAT0_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8452586.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2974..3435
                     /gene="mscL"
                     /locus_tag="ACHAT0_002693"
     CDS             2974..3435
                     /gene="mscL"
                     /locus_tag="ACHAT0_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8452587.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3494..3940)
                     /locus_tag="ACHAT0_002694"
     CDS             complement(3494..3940)
                     /locus_tag="ACHAT0_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8452588.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1136 a          817 c          707 g         1356 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaatac tggtttattt
      181 tggtctcatg taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag
      241 tacgtctttc atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc
      301 aaataaactt tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata
      361 ttgtccaagc attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc
      421 agcgaatcca agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc
      481 tccgctgaag tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc
      541 atcgataatt ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat
      601 ttggaacaac agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa
      661 tgtgtctaat cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc
      721 actttgtttg atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg
      781 agggaaattt tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat
      841 cttaccaact acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata
      901 caagtcttga gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa
      961 tgggtgtgta ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc
     1021 ttcatacatt tggatttcct gaccgatgat tcctttttcc ttgtttacag tttcttctgt
     1081 gaagtaaggt gcttgtacaa aatcgatcag agtcgtcaga ttcttttcta cctgatccgt
     1141 cgtagaaaac agatagctgg ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg
     1201 tttcccaaat aattgaaaga catcaccatc ttctttttca aacaacttat gttctaaaaa
     1261 atgggcaatt ccatctggaa ctttcacctt ttctttttca ccatagggaa taaattcgtt
     1321 atcgatcgaa ccataattcg tactgaacaa accatatgtc ttgtgatagt catttttagg
     1381 caacaaatac acggtcaaac cgtttggcag tacttcatga taaagtgttt cattgatttg
     1441 ctcatattct gttttattca tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt
     1501 cgatttgctc agctacttgt tgtatctctt tgatcgttac gctctccata cgtttcaacc
     1561 attcttcatc agataatttt gtttccggca gccagctgtc caaaaaagaa gcttcgattg
     1621 ccgcttgcgg agagtctaga gaaagcagat attggtttct cagcattgct tttgtctgag
     1681 acagttccaa ctctgttatt tcaccatttc gcaaactttc taattgctcg tgaatcagat
     1741 gaagtacttg atttcggttc ttttcatcga taccggtttg gacactcatg aatcctcgga
     1801 acgtatccac gctactagag gcataataag ctagactttc tttctctctg acattcatga
     1861 aaagtttaga gtgaggaaaa ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt
     1921 ctggttcatc gtaatacaca tttgtctgat aagctaggtt cagctttgct tgaacgattg
     1981 gctcacgtac ttgtcgttcc ttcacgatat tcacttgagg ttgggtataa aacagatctg
     2041 gataaactgt ttcccgcgac tcaaatggta attgcccaac tgcttcagct gctttgtccg
     2101 gatcaacatc gccgacaaca aaaatgtcga tttgatcatt tgccatcatt tcttgatagt
     2161 aggacgctag cgaactagca gtgatcttgt ccaaatcttc gactacgccc aaccctggga
     2221 tcttttgttc cgggctgttt tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt
     2281 gtttatcttc cttcaaactt tccaaatata gacgcagatt gttcttttcc aagtcaaacg
     2341 tcaactgatc aaactgatga tttttaatat ttggataaaa taaaacctct tttaaaaaat
     2401 ctaccgcttg agaaaaaagg tttggatcat tgacatattc accattcaca aaactgatcc
     2461 ctgcgtttag ccaatgtaaa tttccttttt ttctaactga taatccaaag cttgcgccat
     2521 aaagctctgc aagttgcgca cttaactttg tctgatccgg ataattcaaa ctatttgttt
     2581 ccaacatgct gctgagaagt gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa
     2641 aacggataaa aatccggatc gttttgtatt gttttgttgg taagacatga agattcacgc
     2701 cttctgccaa ttgatacacc atatttatat gctccttttc acagttgatt gcccgcttac
     2761 tcttttaatg ggtcaacctg ccttttttac ttattcctct gcttgaaatt tataagttca
     2821 ggcaattcgc cgttttttct aaatgatgtt acactttata gtatagcata gatgtttttt
     2881 atcaaaagaa acagacggct aaaagcgtat attgtttttt tattttttat ttttcgatat
     2941 actaaagaca aataaaagaa gggagggtca ttcatgatca aagaattcaa agagttcatc
     3001 atgcgtggag atgtcttgga tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc
     3061 atcgtaaacc aagtggtgca aggcttgatc acaccactaa taggttggtt tgtcgcaatg
     3121 atcacaggta cttctgattt agaaggagca ctttcaattc ttgactggtc gccgacaaaa
     3181 ggcgtcacat ttgcgtttgg taacgttatt agtgctatca ttacatttct gatcactggt
     3241 tttgtcttat tcttagttgt caaagcggca aaccgtgcga agaatttacg ctccaaagaa
     3301 gagcaagtgg aagtggaaga aactcctact gcagaagatt atcttagtga catacgcgat
     3361 cttcttgcca aacaagtggc agaagacaat acacatccag tagagcaaac aaatctttcc
     3421 gatacttcta aataataaag aatctaaaaa aaagaaagcc gattttcagt gtcggctttc
     3481 tttttttaga ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat
     3541 cttccaaata cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt
     3601 attcttgtgc gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg
     3661 tttcttgaac aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac
     3721 gtccaaaatg gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat
     3781 cagtatcttg aaaaaacgta tgccaagctt gtccatcaat gtcatcaact tcttgataag
     3841 gacactgttg ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg
     3901 ctgttgacaa ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt
     3961 ttttattctt agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatct
//
LOCUS       DAYYAR010000183         4013 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000183 DAYYAR010000000
VERSION     DAYYAR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.186"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHAT0_002695"
     CDS             85..1434
                     /locus_tag="ACHAT0_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8452589.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHAT0_002696"
     CDS             complement(1562..2152)
                     /locus_tag="ACHAT0_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8452590.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHAT0_002697"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHAT0_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8452591.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1148 a          741 c          784 g         1340 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tct
//
LOCUS       DAYYAR010000184         4012 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000184 DAYYAR010000000
VERSION     DAYYAR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.187"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..1399
                     /locus_tag="ACHAT0_002698"
     CDS             77..1399
                     /locus_tag="ACHAT0_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8452592.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(1461..1892)
                     /locus_tag="ACHAT0_002699"
     CDS             complement(1461..1892)
                     /locus_tag="ACHAT0_002699"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8452593.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(1956..2792)
                     /locus_tag="ACHAT0_002700"
     CDS             complement(1956..2792)
                     /locus_tag="ACHAT0_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8452594.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(3534..>4012)
                     /locus_tag="ACHAT0_002701"
     CDS             complement(3534..>4012)
                     /locus_tag="ACHAT0_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452595.1"
                     /translation="SECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMN
                     SLTLTSLKPFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYL
                     HKKVILEGEVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
BASE COUNT         1199 a          812 c          701 g         1300 t
ORIGIN      
        1 ccagccaact actgctcttt tgccgcttct ctttggcttt tttcatacga tacagaataa
       61 gaaaggatct gtaaaaatgg cacaaaaaac aacaaccgca actgcagttg aggaaaagaa
      121 aaaagaaggg ttttggtcaa caaaaattac cggcgtaagt ttgccccttt atttgatcat
      181 ggtccttgtc ctgatcattg caatggcttt aggaaaatta ccgacgaata tgattggccc
      241 aatcgctgtt ttggtcattt tcggtaattt atttcatttt atcggaacaa aaatccctat
      301 cgtcaaaagt tatttaggtg gtggctccgt ttttgcgatc tttgcctctg ccgcgattgc
      361 tacatttggg atcttacctg agtatgtagt aaaatccaca gaaaactttg tcagcaatat
      421 gggcttttta gacttttata ttgctgcctt gattactggt agcatccttg gtatgaaccg
      481 taatctattg atgaaagctt ctgttcgttt tattcccgtt gcacttattt caatggtcgt
      541 ctcctttttt gccgtaggac tcgtaggtat gcttatcgga aatggttttg caaattccgt
      601 tttatatatc tcattgcccg caatggctgg tggtgttgga gccggagctg tacctttatc
      661 aacgatttat gcaaatgttt taggtacgga cgcttcttct atcatttccc gcctgattcc
      721 tgcgacagca atgacaaatg tcctagctat cattggtgca gcgttagttg ctcgtgcggg
      781 acaatcaatg ccaaaattca acggtaatgg taaattaatg gaaaaaggcg accttggcga
      841 tggaaaacct agaaatgtaa aaccttccat tccacaatta ggtgtcggtt tgatggtatc
      901 attgactttc ttcatcttag gacaaatctg taactatttt gttcctaaag tccatgctta
      961 cgcattcatg atcattatcg ttgttatctg taaaatcgca aacctattac cagaatatta
     1021 cgaagatgca gctatcatgt tcaacaactt gattgtcaaa aacttgacag cagctgttct
     1081 tgccggtatc ggtattgcgt tattgaatct aaatgtattg gcttctgctt tgacatggca
     1141 atttgttgtc ttatgtttga caagtgttat cgcgatttca gtagtttcag gatttgtcgg
     1201 tcgtttgttt ggtttatatc caatcgaatc aactattact gctggcttat gcaacaactc
     1261 gatgggtggt acaggaaatg tagctgtctt atctgcagct aatcggatgg aacttattgc
     1321 ttttgctcaa atgggcaatc ggcttggtgg tgccatcgtt ttgatcatct caggtttctt
     1381 gatgcaatta ctttcttagt caaaaaaaat acctgatcat caagtatcgg cttgatgacc
     1441 aggtattttt tgataaaaat ttacaggatt ttcaaaaatc ttttttgcat tttatcggaa
     1501 tgataaccga tgcgttcata aaatcggtgt gcttctactc tgtattctgc agaattaagt
     1561 cttattgctg atattcctcg tcttttcgct tcttctacgc cagtcatcaa ggcctttccg
     1621 attcctcttt tttcagcatg tttagacaca gcaagtgcca taatattgaa catcggatcg
     1681 ctgaaaatcg attcatagga ctccgcttgt acatagccag ctgctttttt agaagtagga
     1741 tcttcataga ccaagaaaaa atttttttct ggctccttca acagtttctt catttgtctc
     1801 accgtaacgg ttaatggtac atcatatcct aattgttccg cattgatttc tcgtagtgct
     1861 tctgcatctc ctaaacgcat ttcccgcaac atccaaatca ccagccaatt tttatttttt
     1921 taaatgaatt tttttatcac agtatactcc ttttttcatt tagaacaata tgtttcagtg
     1981 attttgactt gcgttagtct agattctcct aatgtttgta agatgacgaa agcattgtaa
     2041 agattgatca aaagaaccac gaggacaatt acttgaaaaa taaaggtcac tttttttgga
     2101 gacaaaaccc tgctaaatct tgctccccac aaacctccaa taacagcagc ggggataaca
     2161 taaaacaaca tagataaatc aaagcgcata aagctacttg ttaaagcaat cgttaccaat
     2221 tttgccagtt gagaaaagaa aatcgtagat agagaatata gtgttgcttc ttttattggc
     2281 aatgcaaaca tcagcatcaa caaagaaaca ttgactggtc caccaccaat acctaaaaaa
     2341 ctagctaaaa agcccagaac ccccccacat ataaggtacc aagtccatga cgtcaaatga
     2401 aattttggct gatgatgctt ggtgtagaag aacgcaaaaa ccaaggtcaa taccgtcaaa
     2461 aaaatttgga tcagctgcac atgcttctcg ttttcaaata actgtaaaaa aacttcgaat
     2521 acaatgttgc cgactactcc tccaacaaca gctccactag atacccaacc gacaatctgc
     2581 caattcagtg atttcccgct tgatacttgt cgcacagtcg aaacaatgga catcgtgaat
     2641 accgcaactg ttgagtaaaa agaaatccct gcaacagaat gggcgccgat aagatcaaga
     2701 atcggtttga tcaacacgcc tccacccatc ccggaaatag cgccaactgt gttcgccaga
     2761 acgatcacca caaaataaat cagaccaatc atcttgtatt tcctttctct tgtttatatc
     2821 ttttagcgat tgttactctt ttcttttatt atacagtaaa agaaacaaac gaggcttcga
     2881 aagcttttct actttttaag gaaatttagt gctaaacgaa aagaggttgt gaaaaagctg
     2941 tcctgcatca agtaataaga acagccaaac aaaaatagct cctcataatt ttcgtttggt
     3001 tgaacttatt accgcagatg caagcttttg aacaccgttt aagaaagagg tcgtgagcct
     3061 gccgttcagc tccgagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgttgaa
     3121 tattgtgact tgtgaccgag gagttggcag gagaacaccg tttaaggaag agactgtgaa
     3181 gtcaacgttt tgacctgatt attagagaaa caaatggcaa aatagttctt catatttcgc
     3241 cgtttttcga gcgaatgccg aggggcagct gattgaacat cgtttatttg gaaaaaggga
     3301 ttgtgacaag acgtttgtca caatcccttt ttttatatct tattaattca ttcttttgtt
     3361 ctattcttct tcttccgcca tgaatgagaa acccatatca aagaactata aaggttatca
     3421 aaacagcgtt tatgactaaa tgaattagag ttatctatca ctatttttca agcgatttaa
     3481 accaaatggc atacaatatg gcatacaaaa acttcaataa gccgtggggg tttttaaatt
     3541 tcttcttgtt ctttaaaatc aatgagtgga gctacataat ttgaatccac tttttcaaac
     3601 gtcacttttt gcatcgacga atgttttctt tcccaagata acgtaaatag atttgactct
     3661 ggacttataa agtctacttc accttctagt attactttct tatgcaaata ttcctctaat
     3721 aaatatagac ttgctaaagc atacaacaca ttttccaaat ttgcttcctt ataatttgaa
     3781 gaacgaccat gttttactct gttatatatt ttccaccaat ctggtgtatc ttcaggccac
     3841 ccatctccaa aaggttttag tgaagtcaat gttaaagaat tcataaccct aacaccatta
     3901 tccaaacaca ttaattcgct agatttaata acagctttaa aatcagttat gcttttattg
     3961 ctactgctat caaatccgca aagttctttg aaaataatct cacactccga ac
//
LOCUS       DAYYAR010000185         3993 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000185 DAYYAR010000000
VERSION     DAYYAR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.188"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..893)
                     /locus_tag="ACHAT0_002702"
     CDS             complement(<1..893)
                     /locus_tag="ACHAT0_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFM8452596.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTL"
     gene            complement(897..1307)
                     /locus_tag="ACHAT0_002703"
     CDS             complement(897..1307)
                     /locus_tag="ACHAT0_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8452597.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1325..1816)
                     /locus_tag="ACHAT0_002704"
     CDS             complement(1325..1816)
                     /locus_tag="ACHAT0_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8452598.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1826..2617)
                     /locus_tag="ACHAT0_002705"
     CDS             complement(1826..2617)
                     /locus_tag="ACHAT0_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8452599.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2610..3383)
                     /locus_tag="ACHAT0_002706"
     CDS             complement(2610..3383)
                     /locus_tag="ACHAT0_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8452600.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(3539..>3993)
                     /locus_tag="ACHAT0_002707"
     CDS             complement(3539..>3993)
                     /locus_tag="ACHAT0_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8452601.1"
                     /translation="DCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGAS
                     TKLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNE
                     HLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1252 a          825 c          539 g         1377 t
ORIGIN      
        1 agagttttgt ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag
       61 ttattttgat cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt
      121 tcattagttc cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact
      181 ggtgatttcc aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca
      241 caatactctg aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca
      301 atcatgttaa attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct
      361 tcggattcaa aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct
      421 cctagtatca aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa
      481 tcattcttat catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa
      541 tgaattaaat ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta
      601 gctttgcgat tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt
      661 ccagcgtaaa tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat
      721 tcttcaaatt tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa
      781 ggtttaggat ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa
      841 ggcatgcaat ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac
      901 tcatcctctt cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt
      961 cttagatttt cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca
     1021 atcaaaattc ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt
     1081 cttttagcaa attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct
     1141 tgatttttta cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca
     1201 tctccaagtg ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct
     1261 acaaatccaa tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct
     1321 gttcttaata aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg
     1381 tcacttcaac tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat
     1441 ttatcgtcgg acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac
     1501 ttgttattgg taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa
     1561 taagaacttt ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg
     1621 taattattga agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt
     1681 catcattggc tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc
     1741 attgtgttgc tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt
     1801 tcattttttc atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag
     1861 aaactactaa gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat
     1921 acacaattgc tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat
     1981 ttccggtcat tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg
     2041 tggcaatgag tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact
     2101 ttaattttgt ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata
     2161 aaggataagc agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa
     2221 aactaccttc caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta
     2281 caaatacact atctccaatt cccgctaaag gacccattag acctgttttc agagcactta
     2341 ctgcttccaa agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt
     2401 aaggcgctgt atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca
     2461 ttccttcagg atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac
     2521 ccaacccttc cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa
     2581 ttacttgttt gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca
     2641 gctggtgcta cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg
     2701 ccaagtaacg aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact
     2761 aacaaatagg caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca
     2821 gcaggtaata gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg
     2881 attgcacttc ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat
     2941 acaaagcttg tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct
     3001 ttttgtgcga atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca
     3061 tctagttgta caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc
     3121 tggttagttg agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact
     3181 gatgcaccac caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa
     3241 agtccagttt gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg
     3301 gaggttccga ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact
     3361 aaaaatgttt gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc
     3421 aattactgtg ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa
     3481 atgagcgtct aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt
     3541 agaaaatagt ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa
     3601 tacagtaaat atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa
     3661 atagttgcgc tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt
     3721 caatcattcc agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat
     3781 cgtaatattt ttcatacaaa tatagtactt tttctatctc attgaatact tttttggaat
     3841 ctaaaaagcg aagcttagta ctagcgcctt caatagtaaa gagtttaact aattcattta
     3901 agcagaactc acagctatcc gagtccatat acttatttaa tttttctaag gtttgattcc
     3961 cattaattag attttctaaa tttaaacaat cga
//
LOCUS       DAYYAR010000186         3967 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000186 DAYYAR010000000
VERSION     DAYYAR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.189"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..125
                     /locus_tag="ACHAT0_002708"
     CDS             <1..125
                     /locus_tag="ACHAT0_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8452602.1"
                     /translation="NQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(303..2300)
                     /gene="tkt"
                     /locus_tag="ACHAT0_002709"
     CDS             complement(303..2300)
                     /gene="tkt"
                     /locus_tag="ACHAT0_002709"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8452603.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2402..2653)
                     /locus_tag="ACHAT0_002710"
     CDS             complement(2402..2653)
                     /locus_tag="ACHAT0_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8452604.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2800..3423
                     /gene="lexA"
                     /locus_tag="ACHAT0_002711"
     CDS             2800..3423
                     /gene="lexA"
                     /locus_tag="ACHAT0_002711"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8452605.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3603..3821
                     /locus_tag="ACHAT0_002712"
     CDS             3603..3821
                     /locus_tag="ACHAT0_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452606.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1111 a          840 c          730 g         1286 t
ORIGIN      
        1 tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct agaaaatcac
       61 taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag ttttgtctcg
      121 cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat tctttgtttt
      181 gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc ttctaacaca
      241 taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac aattgcttgg
      301 atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc aaattcttct
      361 aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct
      421 gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac acgtttcttc
      481 actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa gaggtcaaag
      541 cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt ttgagctttc
      601 acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt ttctccttgt
      661 gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc ttttgtagaa
      721 ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc tgttgtctcc
      781 atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat cacttgaaca
      841 ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct
      901 ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg gattgctgct
      961 aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc gtagatacga
     1021 gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc gcggacaccg
     1081 aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc agccaccatt
     1141 gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat agctttcgat
     1201 aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc aacttcataa
     1261 gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc ttcaaattgt
     1321 tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca tgcatcttca
     1381 gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc ttctggaaca
     1441 gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc atcttcacca
     1501 agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc gatgactgtt
     1561 tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat cgctttagag
     1621 attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata tgcttcatag
     1681 cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga aatatcgttt
     1741 gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga acttgcttct
     1801 tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata gtgatccatc
     1861 acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat tgccataccg
     1921 acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc atctgtatga
     1981 tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt caaatcatcg
     2041 attgtcactt gatagccagc taaatgaagc aagctataca acattgctga cccatgtcct
     2101 gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg atttactttc
     2161 aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa tccaggatgt
     2221 cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt aacgccaagt
     2281 tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt ttacctttat
     2341 ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc agtatttttt
     2401 actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct tttaattttt
     2461 ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata tgatgacgca
     2521 taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct tgttcttcgg
     2581 cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc tctatttttt
     2641 caggtgacag catgctcagc cctcctttta ctctccttca tattatataa aaactgaaaa
     2701 aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc gctttttata
     2761 tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca aactgaatca
     2821 agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg atacccgccg
     2881 actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt tcacggtcat
     2941 cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc tcgtgcaatc
     3001 gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat tccagtactt
     3061 ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga cttctttcca
     3121 atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat tcgtggagat
     3181 agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa gcaagaaact
     3241 gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac ttgcaaacga
     3301 ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct cgaaccaatc
     3361 atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag tcatatctat
     3421 taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg gtctcaaact
     3481 cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt tacaaaccga
     3541 acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag aggtgagaat
     3601 tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta ggtattatta
     3661 ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt actttgtggt
     3721 tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt catgaagatc
     3781 atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg actgctaatt
     3841 attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt cacatccttt
     3901 ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat tgcggaaaaa
     3961 gtgattt
//
LOCUS       DAYYAR010000187         3961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000187 DAYYAR010000000
VERSION     DAYYAR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.190"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..177)
                     /locus_tag="ACHAT0_002713"
     CDS             complement(28..177)
                     /locus_tag="ACHAT0_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452607.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1145..1471
                     /locus_tag="ACHAT0_002714"
     CDS             1145..1471
                     /locus_tag="ACHAT0_002714"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8452608.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1479..2387
                     /locus_tag="ACHAT0_002715"
     CDS             1479..2387
                     /locus_tag="ACHAT0_002715"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8452609.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3013..3267
                     /locus_tag="ACHAT0_002716"
     CDS             3013..3267
                     /locus_tag="ACHAT0_002716"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFM8452610.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3809..>3961
                     /locus_tag="ACHAT0_002717"
     CDS             3809..>3961
                     /locus_tag="ACHAT0_002717"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452611.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKK"
BASE COUNT         1248 a          760 c          681 g         1272 t
ORIGIN      
        1 aatctttacg ttccagtttt attctttcta aaaaatatac tcacttcttt tcttagactt
       61 ctttttttga ttctcctctt tatggtcttg tccttttttg ccttcaaaat atttaaataa
      121 acccctttca aaagcatttc taggttttgg acaataagga atgacttttt tcttcatata
      181 aatacctcat ttaactattt gatatcatca cattatcaaa aaaaataggt tttttattaa
      241 aactatcttg acctttcggg agatttcact gtgatttatt tttattccca tacttttttc
      301 gttggtcact ttcgtatacc taatctaagc gatcatcggc gacggtgctt ttacaacgaa
      361 tggcaggaca aaacaagggg cggaaaaact tttttgccta tttcataaaa aactttttcc
      421 tacagcaatc ccttgttttg tcctaccaca cgttgtgaac ccaaagcacg ccgacgaacg
      481 cttagctata cgaaaatgac ctccaaaaac aaaaaagtga ggtgaataga aacaaaaaga
      541 aattgaaatc tcccaaaatg tcgtccgcct cggagacaac ttgctgctga aaatggttgg
      601 ttaattcatt taagtattaa ataagataaa tcatcaaatt aatcggaaaa atagcatgat
      661 tggccttatc aaaaataaac tgtaaaaact tatatttcta tgagtggtgg aacaatgcta
      721 ataccaaatt aatttatcga ttacccacct gcacaaacca aagatagcat tattaggaaa
      781 cgaatcaatc agcgaatcca gcattataat gaggatacta aaaataaagt tttcataatt
      841 ataaatccgc cttcctacga aaggcaccaa tgaggtgatg aaaaccttag agcgctttct
      901 ttcttttttt attccgtgtt accctttcct tgcaggtccc tggtacacta cagaacccga
      961 ggaggtgagt ggcggggctg tatagcggca accccgcata attatatggt gtcggtcatc
     1021 cgttaaggca tcaatgaatg gcgcaaaccc gtagcccgta aacttatctc ttccgaagtt
     1081 gactcgattt cgccggatga ccagtcccta ccaaacctgt actatttttt tacaggaggt
     1141 tgctatgttc aaaatctttc gtaaaaattg ttgtggcttg gatgtccaca aaacgtggat
     1201 ctatgcgtgt atcggaatca cagatgcgaa tggtcgtaca gtgtataagc aagctcgctt
     1261 ctcttccttt acaaaaggtc tcaatgaatt agctgaatgg cttgctaaat acaactgtaa
     1321 ggatgtgtgt ttggaatctt ccggtaaatt ttggattcct gtttttaata ttcttgaaaa
     1381 aacttgtttt gtcacactgg ctcatccgaa gtacaccaaa ccccagaaag gaaataaaac
     1441 agatcgcaaa gatgcaaaaa tggatttgtg atctctttat gtgcgacatg atcaaaccca
     1501 gttttattcc tggtcttgaa atccgccagc ttcgtgatct aatgcgttat cggacgaaac
     1561 tgaccaacat gaaaacaagt gaaaagaatc gtgctttaaa ctgcttaacc gtttctaatc
     1621 tgaaactaga tgatgttttt agtgatgtgt tcggaaaatc atccacttct atcatccaat
     1681 acttgttaga acatcccggt gaatcatttg atgttcgacc atttatccat gcccgttgta
     1741 aaactcccgt tgaagaaatc caagcggcag ttgagggggc gatttctgta cctcaagcga
     1801 ttaagcttcg ccaatgcctc aaccatatta acgagattga gaagcatcga gaagagatca
     1861 atctagagat tcaagctctt gcagaaccct tctcccctgt tttggaacta cttcgtacca
     1921 taccgggctt agacactcag ccaataacag cgattgcgat tctttcagaa ataggaccgg
     1981 atatgtccgt gtttccaact tcgaaacact ttatttcttg ggctggatgc tgtccaagaa
     2041 acgatcgcag tgcccgacga acaaaatcaa cacgtatctc tcgtgcaggg cagtcgttga
     2101 aaccgctact tgtacagatt gccaatgctc ttctcagatc caaagaacat ccagaattta
     2161 aaaatcgcta tcgtagaatc aaagcgaatc gtggacacaa gaaagctatc attgctattt
     2221 gtaagatgct cctgaccgct atctggaatg tcttatcaaa ggtggaaccc tattctgcta
     2281 aaggatatct ggagccaaaa cctgcgaaag aagagaaagt tctgacgcaa tcccaagctc
     2341 tggacctttt aagaaaacgc ggatatacca ttatagatag tgcttaaacc aaagttacgt
     2401 tgattcttcc taaatagact cgttgtcttc catttcaaaa attttgaaaa cggtaggctt
     2461 atttagtgta ccgttcttca ggtatcttca aatagattta ttttcaaact tatttccttt
     2521 cttcttttac tctcctatag cttagcattt aagtgggcac tcgtgtcacc ttgctagata
     2581 gctatgaatg ccgtaataag cctcctagta tataggcaga ctgccaatta taactgattc
     2641 aatccacatt atgataaaat ccaccttttc gtttcatatg tgttgaaata tcacatatgt
     2701 caaaagtgca cgagtctttt cattcagtgc tttgtaaata ctcaaaatta ttccttttca
     2761 ctttaaaaaa aagataatta gtacaaaatc agaaatgagg aaagtattag gatggactag
     2821 aaaggagttg ttgaatttgt tgcaacagat tggtcattta tcaattaaaa attaggtgaa
     2881 gttttattca tcactgcagc tgctgtttcc cagagaataa aacgtctaga agaaactggt
     2941 cagttactgt attatcaagc agcaattgac tacgaacaag cttgattact aaccaaagcg
     3001 tttatccacc tgttgattaa atcaaatcaa aaatcaggtt tttatacgtt cataaaagca
     3061 atctccagtg tactcgaatg tgactgtatc ataggtcctt attcgatgtt aatcaaaatg
     3121 gggtttcctt ctaccgaatc attggacagt tttatcaatg atttatcaaa gtttggacga
     3181 accaacaccc aaatcgtttt ctcgactctt gtttccttaa tggtttatca gttctggaat
     3241 gtttatcatg cgcttttatt aaaatagaaa agagcttttt tcatgcgaca aagcagtgat
     3301 catttattgg cacatgcaat ctttgtggat ccattttttg aaaatacgat atttagtttt
     3361 gacccaaaaa tctttaaata cgtatttgat aatttatctt aacaaccaga taattttatt
     3421 cagcaccgta ttatctatat tgaattctcg gaatcaagat aaattttttt ccgtttttgt
     3481 ttctttttta atcttcttct tgttattaat agtgattagg atgatattcc tatattgatc
     3541 tttgataatt agatataatt tttgaataac gttccctgct attcgagcta gaaatacaag
     3601 tacgccatga cctagttagc gacaactact tagagatttc ttttgttctg ggacaacaaa
     3661 atgccatgac aatagcagta aataatgata cttccgtaag gctcgccgta aactggggag
     3721 aggtgaaatt cctatgtgct tacccaaagc ggttcaaaaa ttttaaacta ttattttagg
     3781 agatgatttt ataaaatcga aagcctactt gaataaaaaa caagcctgtg aatttctagg
     3841 aattgataat aatactttag ataagtggat tcgtgtatat aattttccag taataaaaat
     3901 tgatggcgta gttcgctttg acgtagagga tatcaaagat ttcatgaata cattcaaaaa
     3961 a
//
LOCUS       DAYYAR010000188         3888 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000188 DAYYAR010000000
VERSION     DAYYAR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.191"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2808)
                     /gene="brxC"
                     /locus_tag="ACHAT0_002718"
     CDS             complement(<1..2808)
                     /gene="brxC"
                     /locus_tag="ACHAT0_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFM8452612.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYP"
     gene            complement(2833..3408)
                     /locus_tag="ACHAT0_002719"
     CDS             complement(2833..3408)
                     /locus_tag="ACHAT0_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFM8452613.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(3408..>3888)
                     /locus_tag="ACHAT0_002720"
     CDS             complement(3408..>3888)
                     /locus_tag="ACHAT0_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFM8452614.1"
                     /translation="HENIFFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQK
                     LVNLISILMIDDLFNEFMTEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDY
                     TIKRLIAAYQTNLIETGLVRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         1158 a          746 c          538 g         1446 t
ORIGIN      
        1 agggtaggta taagtgtttt gtaaatcaaa ataagagcgt aaattgtcta attgaccttt
       61 taaatatttc ttgaaatttt ccattgtatg ttcattgtta ttttcatcaa caatattacg
      121 actttgaaac agttctctac cgatttcgtc aaccaatttt tttgcttttg gcgaaatcac
      181 cgcgcttggt tcaattttta tcttttcact ttctcgtcgt ttttgaataa tcattacaat
      241 actatttgga tcatccatcc gtgaaatttg tacattgtta taaagtaaat caattttacc
      301 agacatgaaa agtttagcga tgcaccattc cacatcaagt tctgtgaatc catacggttg
      361 ttttgtttca aaacgatcac gaattgtttt catcgtaatc gttaggtgat ttctcttttg
      421 atcattgata aaattgaaaa tttcctgaac tgcttgctca tttgatggat cttcaataat
      481 ttcatttttg ctttctttca ctaaatataa gatatcttct ttttctttag gcgcatcaat
      541 ttggtttaat ttatggtata cttcttcagc cattttctga agtgctgatt ggaattttgc
      601 tttaaagtcg gagcctgtca gttgaatttc tgcaccgtta tagaaaaatt ttgcagattt
      661 caacagttca tttaattgat cgttgatact tgcacttagt tcctgtaatt caccagattt
      721 ttccgaacga atcgcgtcaa agttctttac tacactcgaa taagtcgttg ttaaataaag
      781 attcattcta gcatacattg ctatatcatc tttaaattct ttatctgatg gcaattctac
      841 gaataataag ttacgggaac tcttaagcgc aagttctgcc tcttgacctg ctaaatcaga
      901 gtatggtgaa ataaaattaa ctgtcatttc agcatttcca gcttgcttgt atggacgttg
      961 atctaacact tgtgcataag taaacgtata ccggttttta aatttatttc cacctgacat
     1021 gcctaatttt tcataggcaa aactatttcg gtctagaacg ccgtcaaaaa ttcgtttaga
     1081 tagttctacg atcaaatcac tatctgtaat attttgattt ttaatctcac gagtaatttc
     1141 ctgttcttca tcagtttgga aaatataact atctaagttc ttccgcacaa agttttcctt
     1201 aattaaaagg tccaatgctt cttctactcg ttttttagtt tccaggcggt cttcgttgat
     1261 atttcttatc atcaagctcg taatattttc aacatttggt ttgatggctt gatcaacata
     1321 tttaatcatg aataaaattc gtaagacatt tacgttaaag ttatctatat cagttggtct
     1381 ttgaatatga gaattatttg tagcttgtgt aatcactcgg ctaattgctg tatcaatcca
     1441 acgttctaac gagtaataaa actgatctaa agaaaccaaa ctggttgtat cattttcctt
     1501 atttcgttca gcggcttctt taaacattgc tagtaatgaa cgttcaccag acgataagtt
     1561 tgatccttga tagctgtgat tacgaatctc ggttagaatt tgccccaata aattgaattg
     1621 atagggaata aatggataaa cttctgcgta attttcagca tcttggaaga gctttaattc
     1681 aggactatca acaaagagta atttattttt caattctgtt tgaacagttt caaattgtaa
     1741 ggctaaagaa tcattggcaa actctttttt cttcaaaata cgttctttaa ttacttcatc
     1801 ggcatttgct gaagataaac tcacagttgt ggcaaaacga tccttgatct tagaaaaatc
     1861 attatcagaa ttgttatcaa agtttgttac gtgatcaatc gcttcctgac ttgtcactaa
     1921 tacccaagcc ttaccttcac aaattcgacc caaatcttcc acaacggttt gtaactgcaa
     1981 agtcaactca cgattaccgc cgatatattg tccgacctca tcgactagaa aggcaatatg
     2041 atggtctgct tctttggaat caagatagct tttaactaga tttgcaaagt catcaatgct
     2101 tattaaataa tttcctgatg ctttttctaa aaaattatca atagcggctt cactcataaa
     2161 atcgatttta agtgccgctt ctttgatatc atcttggatc caatcaaact catggcgctc
     2221 ttcttgccat tttgccccat taatctcttc aaaagcagct ttgaatgcat caaacttttc
     2281 ttctttaatt aaccgacgtt caaaatctgc tagatgtgga atcgtgctta atccttgcat
     2341 ttcgttaaat acctttaaaa aaacattcac gattccgttt ttgtcttttt taccacctgc
     2401 atcacttttt gaatcaatat taaacagaat aacatctgta tttatttgtg agcaccgttc
     2461 catcgttgcc aaagtcagtg gatctttgat tttcccatct tctttgaagt aatcaatggc
     2521 tttcttgtca tttatttctc gatttgcgag caagtaagaa ataattttta agaaatgaga
     2581 tttacctgaa ccaaagaacc cctgaatcca aacacccatt ttatctgttg gtgtattgat
     2641 actccgttgg taggcatcaa aaattgtttt aaagtgtttt tgtagttctt tcgtaaccac
     2701 gtattcttct aattcttgtt gaacattttc atcctttgct tgaccgacct taataactcc
     2761 ttgaatattt cggtcaattg gttttgcaaa aatatcttta attagcattt aatctcctac
     2821 cttccatccc aactaaattc taaatgctcg ataataatta tcatctttat tggtattaag
     2881 aattgtcaaa cttaattcat catattcccc tggatacatc atcacaactg gtacgcgatc
     2941 aaatacctga tgcattttat ttaaaataga gtgcgctcta attaatggat ataattcgcc
     3001 aattcctgtc aggaacacaa ctgatttttc tggtgtatgg gcatccacat attcaaaaaa
     3061 tacattttct tcctcaggta aatccatttt caataaacga ccaatgcttt ccactaattt
     3121 ttgtaaacca cgtcgttctt ccatcttaat cgctttttca gtgaaaccgc gtttttctag
     3181 atactctaac ataatcacat agagattata aactttcact tcataattat caccaacaga
     3241 tgttccagat atatcattaa tataatctct tacaattaac tcgtctttcg gatcgtagtc
     3301 aaagatataa tagcctactt cattacctaa accacgattc aataaaaaat cttctttttg
     3361 aattacttca ttcaactttt ccaaatgctc ttgaatcgat aatgacatta taaaattcct
     3421 cccagtgcta gtacaatatc tttacgattc ttgtcaacta accattgaat caatcgttta
     3481 tctaataata tcggagtcat tcgtccatat tctttttccc ctctgactaa tcctgtttca
     3541 attagattcg tttgataagc ggcaataagc cgtttaatcg tataatcttt ccattttgaa
     3601 atatcttctg aacttctccg cttctctgta aaaaaagaac ggtaatcaac tggtgttaat
     3661 tgcaaatttc ctttacgtag attttcagca tatacctcag tcataaattc attaaaaaga
     3721 tcatcaatca taaggatgga aatcaaattc actaactttt gattatctat atttaactga
     3781 ttgaaaagtt gcaatccagc ttcatccagt tgactaactc tccttttcac gttctgatag
     3841 atttcttttt gacggttact gctcactgca aaaaaaatat tttcatgt
//
LOCUS       DAYYAR010000189         3850 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000189 DAYYAR010000000
VERSION     DAYYAR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.192"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1155)
                     /locus_tag="ACHAT0_002721"
     CDS             complement(1..1155)
                     /locus_tag="ACHAT0_002721"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8452615.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1340..3781
                     /locus_tag="ACHAT0_002722"
     CDS             1340..3781
                     /locus_tag="ACHAT0_002722"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8452616.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1231 a          721 c          840 g         1058 t
ORIGIN      
        1 ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga
       61 agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc
      121 gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata
      181 ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta
      241 gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt
      301 tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct
      361 ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct
      421 ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc
      481 ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat
      541 atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca
      601 gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg
      661 gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc
      721 caagctgtaa cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt
      781 gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc
      841 aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt
      901 tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt
      961 ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg
     1021 attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg
     1081 gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc
     1141 aatccctatt ttcatttcaa cacctcttta cttatgataa aaaacttact ttactaaggt
     1201 atcataaaaa gattaaaaga aatataaaaa agagaatatt taaggattgt taaaatttaa
     1261 tcttagaaaa aaataactgg aacaagagct atttgttttt acagcaaaga atggtatatt
     1321 tttgtaggag gtggcttact tgaaagaagt cgttatgata gatgctgcaa gaacgccgat
     1381 aggaaagtat cgcggaagtt taagtccatt tactgcagtt gaattaggca cacttgtgac
     1441 taaaggatta ttagataaaa caaaactgaa aaaagataaa atcgatcaag taatatttgg
     1501 aaatgtcctg caagctggta atggtcaaaa tgttgccaga cagattgctt tgaacagcgg
     1561 tctccctgta gatgtcccag caatgacgat caatgaagtt tgcggatctg gtatgaaagc
     1621 agtgatctta gcacgccagc tgatccaatt aggtgaagca gaacttgtga tagccggcgg
     1681 aacagagagt atgtctcaag cacctatgct gaaacagtat cagtcagaaa caaatgaata
     1741 tggtgaacca atttccagta tggtcaacga cggattgact gacgcatttt caaatgcaca
     1801 tatgggatta accgcagaga atgttgcaac acaattttct gtgagcagag aagagcagga
     1861 tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg gtgtattttc
     1921 tgaggagatc atcccagtca agatttctga tgaagacgtg ttatctgagg atgaagcagt
     1981 tcgtggaaat agtacattgg aaaaactggg cacgttacgt acagtattct cagaagaagg
     2041 aactgtaaca gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg tgatccttgc
     2101 atccaaagaa tacgcagaaa ataataatct gccttattta gcaaccatca aagaagtagc
     2161 ggaggtcggt attgacccaa gtatcatggg aatcgctcca atcaaagcca ttcaaaaatt
     2221 gacagataga tcaggaatga atttatcaac aatcgatctt tttgagatca atgaagcatt
     2281 tgcagcttct tctatcgtag ttagccaaga actgcagtta gatgaagaaa aggtcaatat
     2341 atatggagga gcaatcgcat taggccatcc aattggtgca agcggtgcac gtatcttgac
     2401 tacattagct tacggattgc ttcgtgaaca gaagagatac ggtattgctt cattatgtat
     2461 tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa cacataagga
     2521 tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa cggcgcagtc aattaataga
     2581 aaaaaatgta ttgacacaag aaactgcgtt gatttttcag gaacaaacac tttctgaaga
     2641 gctctccgat cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa tggggatcgc
     2701 gcagaatttt caaatcaatg gcaagaaaaa atggattccg atggcaacag aggaaccatc
     2761 tgtcattgca gctgcaagta atggtgcaaa aatctgtgga aatatctgcg cagaaacacc
     2821 tcagcggctg atgcgagggc aaatcgttct atcaggaaaa tcggagtatc aagcagtgat
     2881 caatgcagtt aatcatcgaa aagaagagct gattctttgt gcaaatgaaa gttatccttc
     2941 aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact cgggaattca tggggagttt
     3001 ccatgcatac ttatctattg attttttagt ggatgtaaaa gatgcaatgg gcgctaacat
     3061 gatcaactcg attcttgaga gtgtggcaaa caagttacgg gagtggttcc ctgaagaaga
     3121 gattctattc agtattttaa gtaattttgc tacagaatca ttggcttctg cctgttgtga
     3181 aatcccattc gaacgcttag ggagaaacaa agaaatcggt gaacaaattg ccaaaaaaat
     3241 ccaacaggca ggggaatatg cgaagcttga cccttaccgt gcagcaacgc acaacaaagg
     3301 gatcatgaat ggtatagaag ctgtagtggc agctacagga aatgatacac gtgcggttag
     3361 cgcttcgatc cacgcctacg cggcaagaaa tggattatac caaggattaa cagactggca
     3421 gatcaaaggg gataaactag ttggaaaact aacggtgcct ttggctgttg ccacagtagg
     3481 cggagcttcc aatatcctgc caaaagcaaa agcatcttta gctatgttag atattgattc
     3541 agccaaggaa ctagcacaag taattgctgc agtagggtta gctcaaaacc tggctgcttt
     3601 gcgtgcatta gttacagaag gaatccaaaa aggccacatg ggacttcaag ctcgttcatt
     3661 agcgatctca attggggcta ttggtgagga aatcgagcaa gttgcgaaaa aattacgtga
     3721 agcagaaaag atgaaccagc agacagctat acaaatattg gaaaaaatcc gggaaaaata
     3781 aaaaagcaat atctttagaa gaataaagca agatttacag ttattttatt tactgcagat
     3841 cttgcttttt
//
LOCUS       DAYYAR010000190         3805 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000190 DAYYAR010000000
VERSION     DAYYAR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.193"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1623)
                     /locus_tag="ACHAT0_002723"
     CDS             complement(<1..1623)
                     /locus_tag="ACHAT0_002723"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8452617.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEY"
     gene            1956..2426
                     /locus_tag="ACHAT0_002724"
     CDS             1956..2426
                     /locus_tag="ACHAT0_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8452618.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2648..3751
                     /locus_tag="ACHAT0_002725"
     CDS             2648..3751
                     /locus_tag="ACHAT0_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8452619.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         1193 a          684 c          798 g         1130 t
ORIGIN      
        1 atattcagga acccatttag cagattcaac agcttctttg atatccgtga tttcttcacg
       61 attcaatttt tgtgcaacaa ctgattttgc gacagtttca gcaatcgttt gagagaattc
      121 agtgattttt gctactggag gcaagatagc tgctcctggt tttgttacat caacgattcc
      181 gcctaatgct cgactagctt gagaaatgat ttcagcattt actcgtgtag ctgttgaagc
      241 aatcaaacca agccctaaac ctgggtacat caacgcatta tttgcttgac cgatttggta
      301 agttacaccg ttatattcca catcagcagc agggatacct gttccaacta aagcttttcc
      361 gtccgtccat tcaatcaaat cttttgcttt tgcttctgcc aattttgttg gattagacaa
      421 aggcatgatg attggacgtg gggtatgagc cgccatttct ttcacgattt cttcagtgaa
      481 tgctccaggt tgggtagaag taccgatcat gatcgttgga tggattgctt ttacaactgc
      541 ttctaagttt gtcaattcat cagcattagt gaattctgag cgtttacgag cgaatgggat
      601 ttgaccagga gtcaatcctt ttgtatcttc aaacaataaa ccctgtttat ctacttgata
      661 gaaatgttgg cgtgcttctt cttcaggtac accttgacgg atcatttcat ctaaaaggat
      721 gtttgcgata cctacaccag cagtaccagc accaaatgtc aagatcgttt gatctttcaa
      781 ttgctcacct gatatattca atccacctaa tacaccagct agtacaacga ttcctgtgcc
      841 ttggatatca tcattgaaag tagtgatttt atcttcgtat ttttctaaga tcgttgcagc
      901 atttcctcgt ccaaaatctt cccaatgtaa taacaattct gggaataatt cagtagctga
      961 ttgaacgaat tgatcaatga attcgtagta agtttcgcct tctacacgag catgacgatt
     1021 ccctaagtat aatggattat tcaataattc ttcgttatta gtaccagcgt cgattgatac
     1081 aggcaagact tgtgcaggat tgataccagc cgcagcagtg tagaccatca atttaccgat
     1141 agcgatgtca acaccgttca caccccagtc acccatacct aagattcctt ctgcatctgt
     1201 cacaacgatc aaacgaatgt cgcgtccatc tgctgcgttt ttcaaacttg ctttgatatt
     1261 ttctggttcg tcgattgaaa gaaatgcagc atcttgtggt ttcaagaaaa tttcgttgta
     1321 ttgttcgatt gaatccgcaa cgacaggatc ataaacgaca ggcatgaatt caactaaatg
     1381 ttcgcccatc aatttataaa acaatgtccg gttcgtattg aatagattca ttaagaagat
     1441 acgtttttcc aaatcggtct gtttgcttaa gtattgaccg taagcttgga cagattgttg
     1501 ttccaatgtt tggacagtac ttggaagcat accagtgatt ccgtattttg cccgttcttc
     1561 ttttgtaaaa gctgttcctt tatttagaaa aggattattt aataagttta gccctgtaag
     1621 catgaatgat tcctcctaag ttttaataca aacgaagtat aggagtcaga tgaggatata
     1681 gtcaaaaaga gcaggtataa taaattttat ttatatgagt aatagagtca aaagaaatca
     1741 aaatagcggt atttgttctt tttatatcaa caatccttgc ttgattacta tgcgtatctc
     1801 ttatatgcta aagaaagaaa agtagaagaa taaaggatat gttagataga taaatagaat
     1861 ctctgaagaa taaatcacgt gatcgtgacg ataaaataca cttacgtcag gtgataaaaa
     1921 atacataagt agaaaactaa gaggaggaac agaaaatgaa agaagttcaa ttgatcagaa
     1981 aaagcgaact atcagaaggt ggttgcaatg catgcggcgt agtagaagca acaagctata
     2041 cattaaagct tggtgccaac aaagcgataa tctctgagtt gacagtaggc ggtcttgtgg
     2101 acagtctggc gcttgctgaa ggttttattg gagaagatat ttatgagatg ttttcagaaa
     2161 tccgtcagtt aaaaaaaggg gaaaactgca tagaggttca tcatgagagc ccgaatgtga
     2221 gattcaaacg tggtgataat gagatgatct tcaataatca tgtgtccgat cacaccgagc
     2281 tatatggaat cgtgaatcaa atactaactg agctttttgg attgggacct tatgcgttca
     2341 aggaagaaaa cggtaatcca aaattaaacg aagaatggca agaaacgatt gaaatacaaa
     2401 gaaacaaccc gcacttattt caataggcat ccacagtatt tcgtaaaagg gactttgaca
     2461 cgacttttgt caaagtccca tcgtttttta tctatttgtg ttctaaacat ttctttcaga
     2521 gtagtaacaa ctggaatgac tgaaatgtaa acagattatt tgcctgtatc aatacggaag
     2581 agtaatataa agtgtgtaaa cgctatcata ttattcattc aattttttat agagggaagg
     2641 cattgaaatg gaaaaaacgt atcaagcagg tacaaatgaa ggaatagtgg attttatcaa
     2701 tatggaagat ttagaaatag ccgcttcaca agtgattcca gctggtggtt atggttacat
     2761 cagcagtggt gctggcgact tatttactta tcaagaaaat gaacgggcat tcaatcatca
     2821 gctgattatt cctcatgtat taaaagatgt agaattaccc gatacaacta cacattttga
     2881 caaagaaacg ctgactgcac caatcatcat ggcaccagtt gctgctcatg gcttagctca
     2941 tgtgaaggcg gagaaagctt ctgcaaaagg tgtagcagat tttgggacga tctatacagc
     3001 aagttcttac gcttcttgta cattagaaga gataagagaa gcaggcgggg agaaagcacc
     3061 gcaatggttt caattttata tgagcaaaga tgatgggatc aatttggata ttcttgaagt
     3121 ggccaagcgt aatggagcaa aagcaatcgt attgacagcg gatgcaacag ttggcggaaa
     3181 ccgtgaaaca gaccgcagaa atgggtttac cttcccactg ccaatgccga tcgttcaggc
     3241 ttatcaatca ggagtaggcc aaacgatgga tgctgtgtac aaatcttcca aacagaagct
     3301 tagtccaaaa gatgtagaat ttattgctgc tcattctgat ttgccagtct atgtgaaagg
     3361 tgtccagtca gaagaagatg tctatcgttc attagaatct ggtgctggcg gaatctgggt
     3421 atctaatcat ggtggccgtc aactagatgg tggtccagca gcgtttgatt cgttgcaata
     3481 tgtggcagaa gcagtagata aacgtgtacc aatcgtgttt gacagcggtg tacgacgcgg
     3541 acagcatgtt ttcaaagcaa tcgcatcagg tgcagatctt gttgctattg gcagacccgt
     3601 tatttatgga ttgtctttag gaggaagtac aggagtccat caagtgtttg atttctttaa
     3661 aacagaattg gaaatggtca tgcaattagc tggaacacaa acagtagaag atatcaaaaa
     3721 aataaaattg cgtgagaatc gttttattta agaaacaaat gacgaaaaaa ataggagtgc
     3781 ggcagatgat gccgtactcc tattt
//
LOCUS       DAYYAR010000191         3802 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000191 DAYYAR010000000
VERSION     DAYYAR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.194"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..433)
                     /locus_tag="ACHAT0_002726"
     CDS             complement(<1..433)
                     /locus_tag="ACHAT0_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8452620.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGS"
     gene            complement(587..1117)
                     /locus_tag="ACHAT0_002727"
     CDS             complement(587..1117)
                     /locus_tag="ACHAT0_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8452621.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(1216..1566)
                     /locus_tag="ACHAT0_002728"
     CDS             complement(1216..1566)
                     /locus_tag="ACHAT0_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8452622.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1723..3252)
                     /gene="ahpF"
                     /locus_tag="ACHAT0_002729"
     CDS             complement(1723..3252)
                     /gene="ahpF"
                     /locus_tag="ACHAT0_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8452623.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(3262..>3802)
                     /gene="ahpC"
                     /locus_tag="ACHAT0_002730"
     CDS             complement(3262..>3802)
                     /gene="ahpC"
                     /locus_tag="ACHAT0_002730"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8452624.1"
                     /translation="EEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFTFVCPTELE
                     DLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSHQISRIFDV
                     LDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRTHPGEVCPA
                     KWKESGETLKPSFDLVGKI"
BASE COUNT         1225 a          787 c          624 g         1166 t
ORIGIN      
        1 ttgatccttc ttttgtattc actacgttta gttcttttaa tacttctact gaaatactga
       61 cagaagttgc cgcaagaata atcccaaaga aaatcgcttc atttcccgaa acttggaatg
      121 ctttgcctgt caaccagcca aaaacaaccg ggaagatgat cccaagcaaa gcaacgaaca
      181 taccaggtcg gaaatatttt ttcaaaagag ataaatcact ttccagacca gctaaaaaca
      241 ttaataagat cacaccaatc tccgaaaagt catggactag aatatcaggt ttgacccaat
      301 tcaatccagc ttggcctagc aaaacaccaa caagtaattg tccaatcact gctgggatac
      361 ctaatctccg cgagatatgt gagccaatcg ttgttgaaat caatatcagg cataaaatac
      421 caataaattc cattctcatc attcctttgt ataaaatata gcccgaactt agcataacat
      481 acattattga aaaaatagga aaatatactc acaaaaaaac caacaatcca aaagattttt
      541 ttggattgtt ggtttttata cggtctattt taataactaa aaatgattaa cgtatagtca
      601 tttttcttat tctgatgaaa ttgataatct cctggatgcc catgaaaatc aaaataaagc
      661 cgaatatttg gaatagaagc atcaaacttt tgaatggatt gaacaataag acgaatccgc
      721 caatcaacaa cagaatacta ctataaacac tccaactttt agcttttgta ttcatgctga
      781 agaacaactg gaacaagctg ttgatgataa cagctaatcc aagaagaatc ggcaggatag
      841 aaacgattgc aggtgcaaaa gcatagacga tcagagctaa gatcaggaaa aaaagaccac
      901 tgaacagacc gatactccaa gaacctgttt tccttttaaa acggtaagcc tcaaaaagat
      961 tcaacaaccc aagcaaaaca agataggcgg tgatcagata accgataaaa tgaaaaacag
     1021 cagaagggtt tagcacaatc gcaatcccag aaataatata aacagcagct cttaataagg
     1081 cgtatttttg aatattttgt ttaatcgtct caaacatata agaaaacctc ctttgactta
     1141 ttttaacgtg aactagtcat tttaccaaat taaatgttac aaaatgagcg aaggagaaga
     1201 atatctctgg aaaaatcaac ttctgaggat cttttccatt tttttgcctt ttgctagctc
     1261 atcgatcatt ttatctaaat aacgaatttc tttcatcaag ttatcctcga tgtcttctac
     1321 tcgtacacca caaacaacac ctgtaatcaa ggaacgtgaa ggattgggga caggggcttc
     1381 cgcaaaaaat tgttcgaaag tcacttgttt ttgtagttga tattctagtt ctgtttggga
     1441 ataacccgtc agccaacaaa tgatttcatc tacttcctct tttatacgat cttttctctc
     1501 cgcttttgct acatacatag gataaacgct tgcaaaactt gtcgtaaaaa tacgattttg
     1561 cgccataatc agtcaccttt ctttttttca aattatttac tacttatagt atataagaaa
     1621 aagagaagaa aaagcgatta ttcaaaaaaa caaaaaggat tgtgacaaaa ttcttgtcac
     1681 catccttttt tccgctctct gactttttac aatcttcata gctcaagtgc gcatcagata
     1741 atcgaatgct cctaaagctg cagtagctcc tgaacccatt gaaacaatga tttgtttgta
     1801 tggactatct gtacaatcac cagctgcgaa aacgccgggg atattcgttg cgccattttt
     1861 ctcggtcact atttcatttc ttggattcat ttcgatcgtt ccatctagcc aatccgtatt
     1921 agggagtaag ccgactagaa tgaatactcc tgaaacagct agtgtatgca tctccatcgt
     1981 acggcgatcg atataggaga gagattctac ctgattcgta ccgctgattt ctttcgtcgc
     2041 tgcatgtttg ataacagtga cattcgcaag agagactagt ttatcctgta agatttgatc
     2101 tgcttttaat tctggtaaga attctagaac atatacatgt tttgctaacc cagccaaatc
     2161 gattgctgct tcaacaccag aattaccacc accgataaca gcaatctctt ttcctttaaa
     2221 taaagggccg tcacagtgtg ggcaataagc aattccttta tttttaaact ctttttcgcc
     2281 tggtacattg atagaacgcc agtgcgcacc tgtcgcgatg atggcggttt ttgctttaag
     2341 tgatataccg ttagctaagc caatattaat aaaatgttgc tctttattta aggaaacggc
     2401 ctgttgattc atcataatgt cgatagggta actacgcaca tgactttcta cttgctgcat
     2461 caattttgga ccttctgtgt aagaggtacc aatcatattt tcaatcccta gtgtttcaac
     2521 gacttgtccg ccgaaagaat cagcgacaag tcctgtacgg attcctttac gtgctgcata
     2581 gatagctgca ctactagctg ctggtccacc gccaattact aatacatcat aaggttcttt
     2641 ttcaaacaac tcttcttcaa tcaaagggcc agtgattttt tcaacaattt gttcgatcgt
     2701 catgcgtccg ctactaaatt cttctccatt taaaaacaca gttggcactg ccatgatttt
     2761 tttatcatcg atttcttttt ggaacatccc accttcaatc atcgtatgag taatatttgg
     2821 atttaataca gctaatatat tcaatgcctg tacaacgtcg gggcaattat gacaagtcaa
     2881 gctaacataa ctgtcaaaat gcaatgtttg tgttattttt ttgattttaa gactcaaaga
     2941 ttcactgatt ttcggtgccc gtccactaat ttgtaacata gccagtaaaa aagaggaaaa
     3001 ttcatgaccg agggggatac cagcaaaaac gattccactt tctttgccaa ctggattgat
     3061 agaaaaacta ggtgtgcgca tcgaatctct tttttcccaa gagattttag gagacatttc
     3121 tgctatttcc ttcgtaaatt cagctagttt ttgactattt tcactattat ccaaactaac
     3181 agaaaaaaca ataggagatt caatcagtgc taaatattgt tcgagatttt gtttagtttc
     3241 tttgtctaac atatccacca cctaaatctt tccaacaaga tcaaaacttg gtttcaatgt
     3301 ttcgccactt tctttccatt ttgccggaca aacttcacct gggtgggtac gtacatattg
     3361 agcagcgcga atcttatcaa tcagtgcact agcgtcacga ccgattccat ctgcattgat
     3421 ttccatagct tgaacgattc cgtctggatc aataataaat gttcctcgct gagctaatcc
     3481 ttgctcttca tccaaaacat caaaaatacg agaaatctga tgagacggat caccgatcat
     3541 cacgtattcg attttaccga tagcatctga tgtatcatgc catgctttgt gagtaaagtg
     3601 ggtatcggta gagcaagaaa agacttcaac acctaaagat ttcaatgtac tgtattgttc
     3661 ttgtaagtct tctaattcag taggacaaac aaaagtaaaa tcagctggat agaagcaaag
     3721 aatactccag ttccccataa gatctttttc actgacttct aaaaattctc cttgatgata
     3781 agcatgtgca ttaaattctt cg
//
LOCUS       DAYYAR010000192         3720 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000192 DAYYAR010000000
VERSION     DAYYAR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.195"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..355)
                     /locus_tag="ACHAT0_002731"
     CDS             complement(<1..355)
                     /locus_tag="ACHAT0_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452625.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(615..1163)
                     /locus_tag="ACHAT0_002732"
     CDS             complement(615..1163)
                     /locus_tag="ACHAT0_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452626.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1164..2021)
                     /locus_tag="ACHAT0_002733"
     CDS             complement(1164..2021)
                     /locus_tag="ACHAT0_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8452627.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2307..2593)
                     /locus_tag="ACHAT0_002734"
                     /pseudo
     CDS             complement(2307..2593)
                     /locus_tag="ACHAT0_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2583..2912)
                     /locus_tag="ACHAT0_002735"
     CDS             complement(2583..2912)
                     /locus_tag="ACHAT0_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8452628.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2989..3261)
                     /locus_tag="ACHAT0_002736"
     CDS             complement(2989..3261)
                     /locus_tag="ACHAT0_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8452629.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3261..3524)
                     /locus_tag="ACHAT0_002737"
     CDS             complement(3261..3524)
                     /locus_tag="ACHAT0_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8452630.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1092 a          754 c          473 g         1401 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat actctatttg agagtcatct aattggctca
      241 taatcctttc tttttggtta gtatctgcag ttacttgttt agtatgagcc atgaacccaa
      301 acatactcac cccaacaacc aatcctaaat atattttctt tttaaacttt ttcatctaca
      361 tctcctccta atagttttat tttagagtta gctctattac ttatttacta actcaacaac
      421 agattatcat acacttgtta atgaaatcac aatatctcat taaaacacac cctaaaatca
      481 taaccgttcc tatgctcatt ttttggattt tttaatcacg taaccagaaa aataaatccc
      541 ctctatttca cgcaaatcaa agaagaacta agtaactacc gtttaaaggt agttacttag
      601 ttcttctttg attattatct aactttatta ttcattcccc accctttgcg cagtgagtct
      661 tcggtatctt gttttatctg actggtagtt cttattattt ctccacgatc atctaattct
      721 ggaacaattg gatatttttc tctgaacaag tctagttctt tcaccacgtc attaaacaaa
      781 tcttgcttat cttcttgcgt aagattctgt ttaatatact catctaaagc ttttctaagc
      841 aattcatctc ttgtcatatt agtctcatac ttttctacta aaactctgga agtcgcattt
      901 aacaaagtcg acagttttaa aggatgcttg gtggaataaa ccttatcaaa ctttctgatt
      961 ggtgcgccaa ctttttttgt tcttcctcta ctctgtcttt caacattgga ggaagttttt
     1021 atttcttgaa cttggtgcac ttctaattgc ttattttcag atggtgtaat tgcttgctgc
     1081 tttttctttg cttctgcttc tttttttatt ttggcgagtc ctttgttgtt cccaaaaata
     1141 tcaagtcctt tgatgtcagc caattactcc acctccgcat accaattctc gtgttcttcc
     1201 aattctttca aaatactgat aaataattcg tggcttttgc gatcccattg ttttaagaga
     1261 cctttctttg tcttctccat cgtaatacct gatccgtcgt atcgttttaa gcgcgcattt
     1321 ttgagtacaa catttttaat caaatgttcc ccataaatct cttgggcttg tttatacatt
     1381 tcctgatcta ctgcgtcacc gctttccaac atgaaaggaa taacaccagc aacatataaa
     1441 tttgtatcaa attctttcac aaaaaagtcc atatactcaa gatattgcat cgcatttctc
     1501 atcgatttta cttgggtctg taagacgacg attgcccaat ccgcaatgta catcgcggat
     1561 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
     1621 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
     1681 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
     1741 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
     1801 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
     1861 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
     1921 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
     1981 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
     2041 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
     2101 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
     2161 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
     2221 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
     2281 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
     2341 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
     2401 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
     2461 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
     2521 tctttcaatg gcttctcttt tagcttttta aatacttaat gacatgtttt gaaggaacga
     2581 tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt
     2641 gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct
     2701 acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta
     2761 aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac
     2821 tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt
     2881 gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta
     2941 attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc
     3001 gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta ccttataaac
     3061 aacccggtgt tgtctattca atcttctaga atacctacct tcatgtgtag gttttaactt
     3121 ctcaaatgat tgggtaggga gatagggatc ttcctttaaa gtctgaataa ccttctcaaa
     3181 ttgaggtttc aaattagact gtttgatttt ctttaaatca gctttagccg agtttttaat
     3241 catcacagaa tagttattca ttataagtta tcccaatcaa tttcatcaac attagtaaat
     3301 ccactatcat ctttttctcg cttacgaaca acacccaaag tgcctatttg ctcaagtaat
     3361 agcgtttctt tgattgcttt ccaatcatcc aaaccaataa gcaccgcatt attttcactg
     3421 cgctcactaa tgatttcaat ttccgtatga ttctcattta catctttcaa caacttataa
     3481 aaatttttcc gtgctgagct aggatttaag actgtttttg ccatatctac tccccctcta
     3541 atttatatct ccattctaac gtacttaatt aagtacgtaa attcgattgt tttgtttaaa
     3601 ttggagtacc aaaacagcat tgtaaagtgt tatccgtttc ctttataccc gtggtgctag
     3661 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
//
LOCUS       DAYYAR010000193         3696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000193 DAYYAR010000000
VERSION     DAYYAR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.196"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1838)
                     /locus_tag="ACHAT0_002738"
     CDS             complement(<1..1838)
                     /locus_tag="ACHAT0_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8452631.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
     gene            complement(1835..2575)
                     /locus_tag="ACHAT0_002739"
     CDS             complement(1835..2575)
                     /locus_tag="ACHAT0_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8452632.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            complement(2565..>3696)
                     /locus_tag="ACHAT0_002740"
     CDS             complement(2565..>3696)
                     /locus_tag="ACHAT0_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8452633.1"
                     /translation="IGKNVFEILKNVILAPVLFITSMISGGWEETKNNMIAVWNNIKE
                     SAINIWESIKNIFVSYFTNIYFAALNIWTGFKLTLINIWNEVVNQAKSIWLNIKYFFI
                     NLWIDIKYFAIQKWIELKFSIIQTWIDLKYNAITTWNNIKQFFKDTWKNIKDTAYNTW
                     ISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVN
                     LFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKN
                     IVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHL
                     HLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
BASE COUNT         1173 a          704 c          438 g         1381 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcac taggataccc accagtagat
       61 tctccacctc ccgatggacc accaccttgt ccaggaatta tttcttttcc tttaactgta
      121 agttttcctt gtacatctaa gtctccaaaa taaattgctt taccatttcc taataaaact
      181 aatccttttc ctactttagg agaaatcaaa atatatttgc cgtctccatt tgtacgaata
      241 actaaagaat tatcttcaat aggggttgga gtagaagccc caggaaaagg attaccaaca
      301 gaatcagttg ttccaatcgt tccaattgaa cccttagaat tccaaaattc cattcctttt
      361 ttagttaact ccattatttt ctttttgttg ttccaaattt ggagtagtcc attaactaat
      421 ttcaatacat ctccagtctt gttaaaagaa ttttgaaaga tatctgcttt gattaatcca
      481 gtttgtataa aattggcatt aaatatacta tctaatgtcc aagctgaatt aaaaggcccc
      541 cgccatcctt tcttagaaaa agcaatccca tttttattca ttcttagtac ttctcttgcc
      601 ttttctaaat ctggattatc cataataaag atgttggaag gtttttcttt tggccataaa
      661 actacatatc ctcctgcact gccttgacct gtaatcattg aagaaacata atcattaaaa
      721 tcactcatat aattattcgt tgcataatct tttaatttat cctgaatagt tacagcttgt
      781 tgctgataaa aagcaacttg aatgtctcct gcttctaatt tcaaagtttt ttcagataaa
      841 gaatcgtatt gtagtccact aacctttgat tcaagataaa tattatattt tttatgatat
      901 attttaaatg tatcaaacag accataattt ctaatcttcg caaattcttt cgcttcttca
      961 ctatcagtca acttatcaaa ttcaaccgta atagaaactt taggcttatc acagccagga
     1021 ttgatcgttt taaagtaatt tttagctatt ttatttaagc tcttaatgtc ttttactcct
     1081 tgttcttctg taaactgaac atgttctgta tagacatcag gatagttatt aatataggca
     1141 ctatctactg gcgaaccata aattcgatta gtcgtaccta cttcactttg aggatcagca
     1201 tatggaataa ttcttgtttt tatacctgtc caatctaatt tgaccttcaa accagacata
     1261 tcttttccat accgaatcgt tccaacatta ttgcgacctc ttcgctttaa caaagaaagc
     1321 ttaaatggtt cacgtttaat ttctccgccc caatattgaa gcaatgatcc ttgttcacca
     1381 gcaatacaat ttaaaacatt tctcgcttca aagattgtgc tagaaacagc cgtaatatca
     1441 gaataaagtc gtatgtcaga ttttttatcc attttagttt caataattga tatcgcttct
     1501 tgaccagttt tagagtccac ttccacaaga gtgactaccc gtcttccaag tcgatttgta
     1561 cgactctgag cataaatagt tactgtattt aaaaaagtat ctatatcttt atcatcaata
     1621 aaaaagatat gatactcttc ttgatcgttt ggctttgctt ttatttgata gtcattttca
     1681 aaatattcat caaatctagt tcctaaagga taatccaact ctaattcata ttttccatta
     1741 gctacttcat atattccaca tcttgtacaa tcctttaaaa ttcctaagcc attcgttgag
     1801 aaatctgttt cagtaggact atatattctt ggtttcatac ttttctccac cacctaggca
     1861 ttatttcaaa tgaatgaata tttttcgtcc atttaatttc attttttcca ggatataaaa
     1921 atggaaaatc taaaaataag gtgacctgat cctgatgttc taaattacca tctaattttc
     1981 gataagcctc ttctaattta gaatctataa ataattctct gtccaacgat ttcaaatcat
     2041 atttatcctt attaatataa aaagaagcat ctccagaacc actcaattta ataagcggtt
     2101 ttgaagaata cttctctggg ttataaattt caaaagtttc tgtttgtcga atagtaaatc
     2161 taccattgta attttcctta aatggtcgaa tactcactgt aaattcaaaa gggacaatat
     2221 ttcctgtttt tcttgttcct ttaaattcag gtccttctat tactacagct tgataaatat
     2281 attgttcatc atagtaaaga ataaaatcac tataagacga catatctaac cattcagtga
     2341 ttcgatcttc ccactcctgc accatatcaa ttgaaggtgc tttatagtaa cactcaatct
     2401 ttctagtcac atttttgtaa tagcctttat caataatgat agaatcattt ccttctcgct
     2461 ctctcaattc aattactcga ctagctgaaa cagaagcagg cctgttttga atatacacat
     2521 taaattcaga agaataatgt tgattaataa aaaattgtcc tctcttaagt tgcatataat
     2581 gttcctccta ctgcaccagc atctcgtttc atttgtcttg tcaattctgt tttaattttt
     2641 gtggtaatct tccgaaccat tgaatcaggt aaatctccat aaacatttaa atgtaaatga
     2701 atttcttttg tttcactagc agatatattt tgctttgttt gtgtaattgg taggtgactt
     2761 attccattta ctacaggttg aacagacggt gtttttacta aatttgtcat ggtttgatct
     2821 aacttacttt gctctttatc aataccaacg ataatacctt gaacaatatt tttaccaacc
     2881 atatcacgca tccatcttga tggagaatga atatctaaag ctcctttaat ccaatttttg
     2941 atattacctg caataccttt gatagtatct ttcaaagcat tccacttttc ttttacacca
     3001 tttataagtc catcaatgat gttcttgcca atttcaaata aattgacttc ccttaacgaa
     3061 ttaaagattt cttttacccg attaatagca tttgaaacac cattttttag atttgtccat
     3121 gcattttcag cagaattaac aattccagta acaatattcc agaaagagtc cttaatgtta
     3181 ttccatgtat taatcatggt attttttata gaaatccacg tattgtatgc cgtatcttta
     3241 atatttttcc aagtatcttt gaaaaactgt ttaatattat tccaagtagt aatggcatta
     3301 tattttaaat cgatccaagt ttgaataata gaaaacttca attcaatcca tttctgaatt
     3361 gcaaaatatt ttatatcaat ccaaagatta ataaaaaaat actttatgtt taaccaaatc
     3421 gatttagctt gatttaccac ttcattccaa atatttatca atgtaagctt gaaccctgtc
     3481 caaatattga gtgcagcaaa ataaatattt gtaaaataac ttacaaatat attctttata
     3541 gattcccaaa tatttatggc actttcttta atattattcc aaacagcaat catattattt
     3601 tttgtttctt cccatccacc agaaatcatg gaggtaataa acaaaaccgg cgcaagtata
     3661 acatttttta aaatctcaaa gacgttttta ccaatc
//
LOCUS       DAYYAR010000194         3591 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000194 DAYYAR010000000
VERSION     DAYYAR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.198"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..1592
                     /locus_tag="ACHAT0_002741"
     CDS             174..1592
                     /locus_tag="ACHAT0_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8452634.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            1663..3132
                     /locus_tag="ACHAT0_002742"
     CDS             1663..3132
                     /locus_tag="ACHAT0_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8452635.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            3142..>3591
                     /locus_tag="ACHAT0_002743"
     CDS             3142..>3591
                     /locus_tag="ACHAT0_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8452636.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQE"
BASE COUNT         1193 a          523 c          737 g         1138 t
ORIGIN      
        1 gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat aagttaaatc
       61 ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga catatgaaac
      121 gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag aaaatggaga
      181 aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat gtttgggtcg
      241 gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa cgtgatacta
      301 gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg aaagattttg
      361 ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag agagaccagt
      421 ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt tatacagggt
      481 ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat gctgagagtg
      541 atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca caagaagggt
      601 atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa gaagaatatt
      661 tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga cgtttagtgg
      721 aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg gctccgggat
      781 tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg tatcttgtct
      841 attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt ttggaaggtc
      901 cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca ggtagaacag
      961 cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat caagaaaatg
     1021 ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat caaaaagagg
     1081 atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata aatttacatg
     1141 atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg cttgtcaaaa
     1201 atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat cttagtgact
     1261 tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat cgcttcaatt
     1321 tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt caatatatgg
     1381 ataaaggact tattagacct atatctttaa agcctaacca atcttataaa ctaaaagtga
     1441 ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac gctcgtgtac
     1501 atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg actgcaataa
     1561 ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg caggtctgaa
     1621 aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta caaaaaaatt
     1681 gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc tacacactgc
     1741 attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga aatgcttgag
     1801 gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt aattttcgga
     1861 acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc taaggaagta
     1921 tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact tcaacgagct
     1981 tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg ggcagcaatg
     2041 gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga tggctcattc
     2101 gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat tgcaaccaca
     2161 tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg cggaaaccct
     2221 attttaggat tacttgtagg atttgttatg attcatccag gactagccaa tcggaatgat
     2281 ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag cattccagca
     2341 gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt agcaaaagtt
     2401 gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt cccaactgtg
     2461 acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg gaattttatc
     2521 ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc ctttggtgct
     2581 tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca tgctattcaa
     2641 ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc tatttggtca
     2701 gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt taagaaaaaa
     2761 tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg tattactgaa
     2821 ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg ctcgtttatt
     2881 ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat tggacaagga
     2941 ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat atttggtaat
     3001 ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata taaagtgttt
     3061 atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa attgccagtt
     3121 caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat aagcgttgta
     3181 gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt cttttcaaaa
     3241 ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt atctccaatc
     3301 gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa aacaaaatca
     3361 ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg tgagggattt
     3421 gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat gaccattaat
     3481 tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt tccagactac
     3541 caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga a
//
LOCUS       DAYYAR010000195         3553 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000195 DAYYAR010000000
VERSION     DAYYAR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.199"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHAT0_002744"
     CDS             <1..156
                     /locus_tag="ACHAT0_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8452637.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="ACHAT0_002745"
     CDS             175..1452
                     /locus_tag="ACHAT0_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8452638.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="ACHAT0_002746"
     CDS             1462..2118
                     /locus_tag="ACHAT0_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8452639.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="ACHAT0_002747"
     CDS             2118..3494
                     /locus_tag="ACHAT0_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8452640.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
BASE COUNT         1458 a          357 c          605 g         1133 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta acc
//
LOCUS       DAYYAR010000196         3514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000196 DAYYAR010000000
VERSION     DAYYAR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.200"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1359
                     /locus_tag="ACHAT0_002748"
     CDS             28..1359
                     /locus_tag="ACHAT0_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8452641.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1325..1675
                     /locus_tag="ACHAT0_002749"
     CDS             1325..1675
                     /locus_tag="ACHAT0_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452642.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1767..2186)
                     /locus_tag="ACHAT0_002750"
     CDS             complement(1767..2186)
                     /locus_tag="ACHAT0_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8452643.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2187..2531)
                     /locus_tag="ACHAT0_002751"
     CDS             complement(2187..2531)
                     /locus_tag="ACHAT0_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8452644.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2881..3477
                     /locus_tag="ACHAT0_002752"
     CDS             2881..3477
                     /locus_tag="ACHAT0_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8452645.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          923 a          640 c          641 g         1310 t
ORIGIN      
        1 taaagaaaat gaaaaagaag gtgtgtaatg gaaaacgaaa ctgacgttat ttatattcat
       61 cctcaaaaac gaatcgtttc acagaaaaga aagtattttt atcttggttt tacaggtgtt
      121 ttttttcttt ttattggctt gctttctaat actcctactg ataattggag tggattgcta
      181 actattctta cctcgccaag caatcttttg acagattatt ttgctttagg tgttttcgga
      241 agcgcattca taaatgtagg gattttgacc ttactttctg tgcttttagc ttatcggcac
      301 aaggtgattt taaacggtcc cttatttgct tcgattctaa cagttaccgg tttctctttt
      361 tttggcaaaa acctttataa ttctatctca atcattttag gtgtgtatct atacgctgta
      421 tttgtcaata aacctttttc tcagtacatc atgatcggac tgtttggtag cgtattatca
      481 ccggtcgtta gttatatcac ttttggtatg cgttttcctt tactgatagg tattttattg
      541 gggaatttag ctggcatagc aattggtata cttcttccgc ccttagctgc tcaaacttta
      601 gttttccatc gtggattcac actttataat attggattca cctctgggtt gattgcgatg
      661 acttttactg ctgttttgag gttattcagc tattcaatcg tcgaaaatac attggttttc
      721 aatgaatatc actttcctct catttggatt atttttggct ttttttcgct gacagtcgga
      781 attggtttct attataattc ctttcggctt tcggggattc gagaaatttt cgattcatct
      841 ggaaaattga caactgattt cattgctaat tctggtattg gtgctacttt gatcaacatg
      901 ggcttagttg gtctgatgct gtcttcttat gttttgctgg ttggcggtca gttaaatgga
      961 cctgtcatcg gtgctatttt atctgcggtg ggtttcagtg cttttggttg tcatttaaaa
     1021 aacagttttc cgatactagt cggaatattt attgcttctt tgtttggaac ttttcatgag
     1081 atcaccagta ctggcatgct tgttgcagct gtgtttggga caggtcttgc gcccataagc
     1141 ggtttttacg gcagttttta cggtgtaata gctggtgtgc tacatattgc tcttgtccat
     1201 aacgtcagca ctttgcacgg aggactaaat ctatataaca gtggtttttc aactggtttc
     1261 gttgcaggta tccttgtacc gatactagat aactttaccg ctgtcagaaa ggaaaaaaag
     1321 acacttggaa aaagaattat caaaaagaat catcgatgag cttcttactt atttatattc
     1381 ccatgaaata actgaaatac gtattggagt agaatttgct gaggaagggc ttttcataga
     1441 gatacagggt acggcagaaa ctcagccaga agatttagat gtacttgctg atttgctgaa
     1501 caatcctcgg gaccctagta tggaaatcta ctatcaagaa ctattaggta atccccgaca
     1561 tgatcgggaa gattatcatc tgttaggtag tttgattgat gacgtggaaa tcatgtatgc
     1621 ccagccgatt atcaatatta agatctttcg acgtgcttct cattcctatt attaacccta
     1681 ttttctattt ataaaagact gaatcaaaaa agcagctcct gaaagttcga ttaaaatcta
     1741 ctttcaggag ctgttcaagc ttgacattat tgattttcca atccttcata cacttcatct
     1801 gttacaggtt ctaaaaactc tgaattacca gcagtgatcg ccaaatgttc aaaccaactg
     1861 tcttttgtcg caccatgcca atgtttaatc cctttgtgcg ttacgacgac atctccagcc
     1921 attaatcgtc ttgctggctt tccttcttcc tgataccagc cttctcctcc ggtcacaagt
     1981 aagatttgat agccgtctgt atggacatgc cagtgattgc gacaacctgg ttcaaaagtt
     2041 acattaccta caccaacaga aacttcggga tcattcacta acatattcag atagctttgc
     2101 ccggtaaata cttgagcgta agcttcgttt ttttcacctg ctggaaaaat cacgccattg
     2161 tgtatttctt cgtgttttgt catcatttac gcctctttct tatcttcgct attttcttct
     2221 aaatagattt cttttgctaa actaaaagct gaccaagctt ttggccattc tacataaaaa
     2281 gctaaatggg taatgatttc tgctatttct tcactagtaa tgccattttg cttgccaatc
     2341 ttcagatgag ctggcaattg ttcaaaattt cctcctgaaa ttaatgcaga tatggtgatg
     2401 atactacgct gatgagcaga taattcttct ctggaccata cttgtccaaa taaaacatca
     2461 tcatttaatt ctgcaaattt tggtgcaaaa tctcctaatt gatctcgtcc tgcggtttgt
     2521 ttttctgcca tttctcttat tctcccttca cgactgttag gtgattcaac tatggttcta
     2581 aggcaacttt attttctatt gtttcttcgc atttcatttt aaatgtagtc tttcagcctg
     2641 tccaatgctt agtttattca gtcgatatgc tttttaagca tagttgctaa cgcttttttt
     2701 attttaaaaa aaacaagtta ttaaataagt ctttagttgg atgttttttt gagatcaatc
     2761 ttttatactt atgatgaact tacaaaattg tttgttttct gtcatatcaa aaaatggcgc
     2821 agttagttgc ttttctagta aagtatagtt aacaaagaac ctctaaggag ttgatgaata
     2881 atgaatattg ctgcctctcg aaaactgata aatttcatat ccattatcgg attaggccta
     2941 tcaatcgcgc tgacgattta ttttattaat ttaggtgttt tcaaagattt gaatgcgtta
     3001 agaggattag taggtgattc catcatttta ggaccgatta tttttgtctt catccaaatc
     3061 ctgcaagtag ttattccaat cattcctgga gggatcagta ctgcagctgg tgtcctgata
     3121 tttggccctt atgctggatt tatctataac tatattggaa tctgcattgg ttcgattatc
     3181 atttttctgc tagggcgtcg ttatggaaag ccattcatac tttccatgat tagcgataag
     3241 acatataaca aatatattgg ctggctggat aatcaaaacc gttttgagaa gctgtttgct
     3301 ctagctattt ttcttccagt tgctcctgac gatgcgttat gtttgatggc tggattgaca
     3361 aacatgtcag tgaaacgata tacactaatc attttgattg ctaaaccact gtcgattttc
     3421 ttatatagta tggccttgat ttatggaggt caatatctaa gtggtcttct tggataatat
     3481 ttattttgta aaattagtca ttgattgaca atgt
//
LOCUS       DAYYAR010000197         3492 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000197 DAYYAR010000000
VERSION     DAYYAR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.201"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(240..1940)
                     /locus_tag="ACHAT0_002753"
     CDS             complement(240..1940)
                     /locus_tag="ACHAT0_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452646.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2447..>3492
                     /locus_tag="ACHAT0_002754"
     CDS             2447..>3492
                     /locus_tag="ACHAT0_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8452647.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKV"
BASE COUNT         1177 a          615 c          697 g         1003 t
ORIGIN      
        1 tgaatggtca aatacaagaa aaggtatttg gtcagtctcc aaaagtcata ttcttcatcg
       61 atcattaaca gacaaagaac tggcccgcca aggatatgag gacatctcct tgaagtacca
      121 gttcgtacac tcaacttatt gaaccgccgt atacgggtcc gtacgtacgg tggtgtgaga
      181 ggggcggaag gtcagttggc tttcccctct actcgatcgg atgtatttat cccttttgcc
      241 taagcacgta aaagcttgta tctatgataa ataaataaac atagcaaggt aaaccagcca
      301 atcaaagtaa gagaaagaat gaaatagaaa tagattggcg tttgataatg aagcgtgagc
      361 tcgttgattc ctttttgctg cttcacgatt ggggtcccga tatctgttac ttcataatca
      421 gtcaatgttt ttccattttg ctgtagcaca gtccgatcgt agacgataac agggatatga
      481 acagttttgt ccccagtgtt tttccacttg attgtcaaaa gaccgcctgg atgtgttttt
      541 ataaaagttg tttcgttttc tagtatttct tcgttatacc ggtcgtattt attgtctttt
      601 gtctccttat agattggcag atagtcaggt gtggtttttt ggaaaacgaa caagctggta
      661 gacatatctt tgctgaagaa tgttttcctt gcttcttccg gcgtatcgaa tagataagta
      721 tgtcgactga caaatgtttc attttcccac ttttccagat gctggtataa gttctggatc
      781 gtctgtgctg aaaaaataag aatgccaaat atacttaaaa gacggaacca cttttgttca
      841 gaccactggt tcagtgaaag ggcaaggtaa agtagaagca atagcgtaaa tggcacgaaa
      901 aatcgaaatg gaaactggat caaactaata aaaggcacat ttttttcgat caatgtcgtc
      961 catggaatca agctagtaga aagaaaaaag aaaacaagac ttgtgtacaa cacgattttt
     1021 tggaataagg tgttcttttg ttgaaagaaa ataaaaagca aaaaacctga taatatcaat
     1081 ataggaaagg ccttagggta atggagccaa taactgccat ccagatctat cgccttttga
     1141 ctcatttctc tgttaacaaa gggaggaaga atcttattcc cactgtaaac aatagctaat
     1201 cctgcccaaa tatttgctgt cagaacaaaa taaagcaaga ccgacttaat cagttgaagc
     1261 agtgcttttc ctttttcagt ctggttaaaa aagtaataaa gataaaaagg gatatacatc
     1321 aaagctaaca tgatcgaaga cagtaaatgg atctgtgtca taagtcccac tgagacagcg
     1381 actttgataa cagggaactt tttatgaagg acgaaatcag caatagggat catacataaa
     1441 ggataaaaac aagtccccca gctagtgaat ccttggttga tagaccagta ttgaatagaa
     1501 tacgtactca tgaataaaac ggctactggt acacttaccc aatttcgaac attgacatat
     1561 ctcagtaaaa tcagcataga aaatcctgaa ataagataaa gaatgaaatt tgaaagaatc
     1621 tgataatgaa accagctacc tgcaagcaac acgatcaatc cttgtaaata agcgaaaaat
     1681 gggccgtaaa cggcattcac gatcctgcca ctttgctgga atccataaag ggaaggaaaa
     1741 taatggaaat ccccatattt aatttgttgc gcggcatcat agaaccgatt gtaatggaat
     1801 ggggcatcag cacccaaaat tagtttatgt gtgaataaat gaggcatgag aaatgccaaa
     1861 gcaatgaaaa aaattgctaa aaatgggcca tattttttca attgtcgaac tcctttgcaa
     1921 actttagata aattcatcat atatcctatg cgagaggtag gcaagaaatt agaaaattca
     1981 tgaaaatatt tatgaatttt acaagaaaaa cataagtgaa agttctaagg agagaggatg
     2041 cgacataagt tttgttgcat ctccttgttt tgaaacagaa tttttcttaa tactcgaaac
     2101 aaagtagctt tttcatgacc tctcggtaaa cggtgttcaa tcagctgctt ctcggtaata
     2161 agctcactag cctagaaaat atgagaagct attttctgac tagcgctctt attactcaaa
     2221 gcataacgca gttttcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt
     2281 aataagccca acccaacaaa aaatatgagt agctattttt ttgggctgta cttattactt
     2341 gatgcaggac agctttttca caacctcatc ggctttcatt ttctattttt ttcctgaacc
     2401 catataataa aagaaagaac aaaaaaactt gcaagggagg acacatgtga agcagagtaa
     2461 gcaaaaacag tctcgtcgac ttttgtttcg tttgttttta ttaatatgta tcgtcgtagg
     2521 gttactgatc gctttatatc cattttatgt ggattcttta aatagtttta ttgatcaaaa
     2581 acgtatcgaa caagtacaaa aacggaccgc agctgaaatt gaagtgcagc gaaagaaaat
     2641 ggaggagaaa aaccaacgat taactgacca aggattcaat cctggtactg atcctttcaa
     2701 tgaaaagaac agaaatgaaa gtacaacatc cagccaatta gaggaatggc tgatcggaag
     2761 tatcaatata cctaagatcc aattaaatat ttcactttat gatcggctca atggtaggat
     2821 ccttgaaaat ggagcaggcg tcttgcaagg aacctctttc ccgcttggtg gaaattcgac
     2881 gcattcagtg atctctgctc acagcggttt acctaatcga cggttattta ccgaattaga
     2941 tcgtttagaa aacggagaca cgtttatttt gactgttttg ggggagaaat tggcttatca
     3001 agttaaaaat atacaagtgg tactgccaga tgacacgtct gttctaacga tttcagaagg
     3061 agaagactta gttaccttgc ttacatgtac gccgtatatg atcaacacgc atcgtttgct
     3121 agtgacagga catcggattc cttataacga atcggtaaaa aaagaagaag gaaaaggaaa
     3181 tcaggaaaga accatgcggc aatggtttat tcttgcagga acgataatag ctgtagtcat
     3241 actgttagtg tttatcggac gactaatcta tcagtataga ttaagtaaaa aagtgcttga
     3301 tttttcattt attattagtg attcgtcagg gaaaccggta aatggtagtt cattcatttt
     3361 gaaacataag aaaaaaacac ttactagaaa tggtgtgccg ttttcagtac aaagcgacca
     3421 tcatgggaaa gtcaagctgg atcaattgcc tggtggaact tatcgaatcg tttcagctga
     3481 tgatcctaaa gt
//
LOCUS       DAYYAR010000198         3476 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000198 DAYYAR010000000
VERSION     DAYYAR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.202"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..492
                     /locus_tag="ACHAT0_002755"
     CDS             <1..492
                     /locus_tag="ACHAT0_002755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8452648.1"
                     /translation="PLMIDAIGKLIHEGYECELDIYGEGGLKTELESYINHNGLTEFV
                     HLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEALAIGVPVIATDCPVGGARMYLGD
                     RNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQKGIKLRKELTSKNISNIWYEYLN
                     QFF"
     gene            511..1935
                     /gene="wzy"
                     /locus_tag="ACHAT0_002756"
     CDS             511..1935
                     /gene="wzy"
                     /locus_tag="ACHAT0_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFM8452649.1"
                     /translation="MKEKINILMFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAIC
                     TWHWRKGDSIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSES
                     DMIMVKWYSCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIV
                     VPIVFVSKLLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKV
                     ITRFVFIIFALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIG
                     IYILNVITKARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNG
                     IYPFANTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNG
                     GYIGGIVYIFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIV
                     KNFVYGVLIILLINFFLQQFNKRL"
     gene            1981..>3476
                     /locus_tag="ACHAT0_002757"
     CDS             1981..>3476
                     /locus_tag="ACHAT0_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8452650.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAVIVFICIILVNVLFYRRYVVKIISKIIVKI"
BASE COUNT         1154 a          442 c          630 g         1250 t
ORIGIN      
        1 cccttaatga ttgatgcaat aggaaaactt attcatgaag gatatgagtg tgaactggat
       61 atttatggag aagggggatt aaaaaccgag cttgaaagtt atatcaacca taatggatta
      121 acagaatttg ttcatctaaa agaatttaca aataaaatac atgaaaagat gcaaaatgcg
      181 gcaggatttg taatttcttc tgattatgaa ggtatatcta attctatgtt agaagcactg
      241 gctattggag tgccagttat cgctacagat tgtccagtag gtggcgcaag aatgtattta
      301 ggtgatagaa atgaaatagg tcatcttgtt gaatgtaata atctagataa gttaacagat
      361 gcaatgcgta gggtactttt ggatagagaa agtgtggtaa gaaaagcgca aaaaggaatt
      421 aaactaagaa aagaactgac atctaaaaat atttcaaata tatggtacga atatttaaac
      481 cagttctttt aaccaaaagg aggatattgt gtgaaagaaa aaataaatat attgatgttt
      541 tcgttaacgt tagtcgcgtt tacaatattt tgcgcttttt cattaaatca gaactattgg
      601 ctaaattggc ttggaatatt gatttactta tatgcaatct gtacatggca ttggagaaaa
      661 ggagattcaa tattttcatt atacacgatt tttttcactc tttttttatt atttagttat
      721 gggcaatgca ttatgtgggc attcggtatt ggaacagatc gtggaatcgg tacaacagta
      781 gtagcttatg gtacggggat tattcctagt gaatcagata tgataatggt aaaatggtat
      841 tcttgtattt ctatgtttgt atttcacata ggagcactct tatttacgag aaaaaccaca
      901 ctaagaaaag ttagttggta tgaatctgga gatgctgatg ccgatagaaa gtttttattt
      961 aaaatagggt gtatcatttc tataatagta gtaccgatag tttttgtttc taaattacta
     1021 gaggtacgta tagcattagt ccatggttat aatgcattat attatggtga ttatgctaca
     1081 caaagtggat atttacaaat tattctatat cttttttttc cagccttaat atgtttatta
     1141 attggtagca acttttcaaa agtaataacg agatttgttt ttattatttt tgctttatat
     1201 tctttattag gaattatgtc aggagacaga ggaagttggt tgtatagctc tattatactc
     1261 atatgggcat acactcaata taaaggaacc tttaattata aaaaattaat aaagtggctt
     1321 attttagctg ttataggaat atatattcta aacgtaataa cgaaagcacg tgatggtgga
     1381 ggactaagta aacttacttt aaaagatttt acatctgtat ttgattcaga tgattctcct
     1441 ttagttgatt ctttttttga aatggggggg actatgagta ttataacttt tttcctaata
     1501 agtggtaatg gaatatatcc ctttgctaat acttatttga cctcactctt aggctctata
     1561 tcaacaagaa tgctatcaat gtttggaata aagtttgttt tattagctga ttggttttct
     1621 caggattatt taggaatcac ttgggggact ggattctcaa tgattgcaga agcctatgtc
     1681 aatggtggat acattggggg tatagtctat atttttatta ttggtatagt catcggtaaa
     1741 atcctttcta atagtataga caaccaagat attgaaaatc atcctatgaa gatgtttttg
     1801 gctataacta cagcaaatat tataatgggt tttcctagag ctgcttcata tttaattgtg
     1861 aaaaattttg tgtatggtgt tttaattata ttgttgataa actttttcct acagcaattt
     1921 aataaacgtc tttaatattt aagcttaata aaaagataaa acaagtcagg agttgcaaaa
     1981 atggaaagaa ctgctcggct caaaaaaaac actatttggg ctctggtgtt tcaaataact
     2041 tctattgtct gtggtatgat cttgctacgt ttgatattaa caaagtacgg atccgaggtt
     2101 aatggaatga ttagctctat taaccaattt ctacaaatta tttctttttt acaattagga
     2161 gtaggcgctg taatgcaatc aactctatac aaaccactag cagaaaagga taatttaatg
     2221 ataagtcaaa tcttagcttc tggagggaag ttttatcggc aaattgcata tattctacta
     2281 gtatacgtgg gtttgttact tgcagtctat tatgttctcg ttcctaatga atttgggaga
     2341 tcatatacaa tggcaatgat tgcaacatta agtattaatt catttgctca atattatttt
     2401 ggtattgtta atactttgtt attaagtgct gaccagaaaa tttatattac gtatattgca
     2461 cagacaatag catttatttc taatacagtt gcatgtgctt tattaattag tctgaacaca
     2521 gagatcctta tggtaaaact tattactgct ataatttatc ttgcttgtcc aatatattat
     2581 agatggtatg tggatcgaaa ctataatata aagaggaatg tgtcatatga taaggaaccg
     2641 atccctcaaa aatggaatgg ggttgcacaa cacgtagctt ctgttgtact agatagtact
     2701 gatgttgtag tgttatctat attttctaac ctgatatatg tatctatata taatgtgtac
     2761 aatttagtaa tatacggagt aaaacagcta ttcatggcta tgacaaatgg cgttcaggcc
     2821 ttactagggg aactttgggt cagaaaagaa atagaagttc tttacaaaac atttgcatgg
     2881 ttcgaatgga ttgtacatac tttgacagtt tttatttttg gctgtaccgg tatactaatc
     2941 gttccattta ttcaagtata tacaagagga gtaacagatg ctaattataa tcaattatca
     3001 ttcgcagctt tacttgtaac tgctaacgca cttcactgtc tgcgtacacc ttatcatgta
     3061 atgataaaag cagctggtaa atataaagaa actcaaaata gttttataat tgcttctttg
     3121 ttaaatttag gtttatcatt aattttggtt aaaccactag ggctagttgg agtagcatta
     3181 ggatctctaa tttcaatcac atatcataca ctttggagtg caaattataa ttcaaaatat
     3241 ttgcttaaat ggccgatgaa aaaattctat aaacagttat ttgtaaacat cttaactgta
     3301 gttctttctt ttggggcgac atcacaattg aaaatgatgt caatcacata tttggattgg
     3361 attgttctcg caattaaatg tgcagttatt gtatttatat gtataatcct agttaatgtt
     3421 ttattttata gaagatatgt agtaaaaata attagtaaaa tcatcgtaaa aataaa
//
LOCUS       DAYYAR010000199         3462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000199 DAYYAR010000000
VERSION     DAYYAR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.203"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..1787
                     /gene="yfcC"
                     /locus_tag="ACHAT0_002758"
     CDS             48..1787
                     /gene="yfcC"
                     /locus_tag="ACHAT0_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8452651.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1944..3128
                     /gene="iadA"
                     /locus_tag="ACHAT0_002759"
     CDS             1944..3128
                     /gene="iadA"
                     /locus_tag="ACHAT0_002759"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8452652.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3239..3442
                     /locus_tag="ACHAT0_002760"
     CDS             3239..3442
                     /locus_tag="ACHAT0_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8452653.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1095 a          582 c          813 g          972 t
ORIGIN      
        1 attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa
       61 attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca
      121 ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt
      181 tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt
      241 tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat
      301 tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat
      361 tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct
      421 ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa
      481 aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa
      541 attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg
      601 cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg
      661 caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat
      721 tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat
      781 tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa
      841 ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg
      901 tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga
      961 ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa
     1021 tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata
     1081 tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag
     1141 agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc
     1201 cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt
     1261 tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg
     1321 atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag
     1381 gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt
     1441 tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca
     1501 acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac
     1561 tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag
     1621 gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac
     1681 ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt
     1741 taggaactat tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg
     1801 taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa
     1861 aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat
     1921 acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag
     1981 attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac
     2041 agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac
     2101 taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt
     2161 atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag
     2221 ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag
     2281 cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc
     2341 cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg
     2401 gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg
     2461 ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca
     2521 atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa
     2581 cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg
     2641 aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc
     2701 ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga
     2761 tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc
     2821 cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc
     2881 tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag
     2941 ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg
     3001 gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg
     3061 ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa
     3121 gtttttaatc aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat
     3181 ctgttgttga tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat
     3241 ggcagattta aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga
     3301 agcatacggc aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact
     3361 aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc
     3421 tcgaaaagac gaaaaaaaat aaaaaccact tattactaat tg
//
LOCUS       DAYYAR010000200         3442 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000200 DAYYAR010000000
VERSION     DAYYAR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.204"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2199)
                     /locus_tag="ACHAT0_002761"
     CDS             complement(<1..2199)
                     /locus_tag="ACHAT0_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFM8452654.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESLSESFNEARSSEPSITQESQIER
                     QEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVRVPRASIGLIEF
                     IRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITFEVAFFQSGGTE
                     PNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGNLGNLLIDGQDV
                     TNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREPISGGVRIAEVT
                     NPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYALEVTDVDLFNE
                     GRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNVINLNENELTYT
                     LHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRFSANNTIGGPTS
                     FTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKVIKTDENGQRLA
                     GAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYLLDATVHDVFIN
                     EYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGETLQTG"
     gene            complement(2871..3139)
                     /locus_tag="ACHAT0_002762"
                     /pseudo
     CDS             complement(2871..3139)
                     /locus_tag="ACHAT0_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          967 a          764 c          544 g         1167 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tacattaaaa gactcacttg tgctttcttc tggtacactg atttctattg agaccgtatt
     1861 tgattgaata tcaccgcttc ttgcggtcaa ttcgtattta cctgaatgct gggaagaata
     1921 agtaatcaga cgttcgtaat cattgagttg cttcacttcc ccttcaatca aatcctcact
     1981 cttatcaatg accaatgcat ttagcaggtc tggtgttcct ctgactcgca actggaactg
     2041 ctcacccaaa gcagcctcct cttgatcaac ttcgatcgtc agcgaacgct ctggctgttc
     2101 tgctaaagac tcagctagaa ccgttatgtt cccaaattga ttgatcacaa taagcataac
     2161 tagaaacata cttaacagtt tatgaaattt gttcctcact attactcccc cttataattt
     2221 tgactaaaca acaaggtacc cgataatttt ctaaaaagat tccccaactc aaaatggctg
     2281 cgttacctcc ctcgtatttt cggttcaact caagcgttgc aatgtaagct gtaaacaaag
     2341 ctaatgattg ccgattgtct tctgttgatt gattcgatag ctttgtatga cttttagttg
     2401 tccgtgtggc ttaaccataa ttatgatttc acgattcctc ctgccagtgc cttgcacaag
     2461 ctggagcgta accctaatag ttattttgtt aatgtactta tatactttta tacttttcca
     2521 ttagaaacaa tcactaaatc gttgatacca aggtcagact ctagaatttc atcagtgagt
     2581 ccctgtattg gttaaccttc atcaatccca aaaaaatata tcaatgctta gcatcaaaat
     2641 gaatatatag tttagaatac ttgggattca tcgataactg atcgcttatt tggccatatt
     2701 atttggcact tggttcaact aaacgcattt aggatgttcc ttcattctcg tcatggatgt
     2761 tctcaaaccg tcattactaa taaactgtcc accgaatcga tggttcattt cttgcatttt
     2821 ccacgtccaa ttactctttt tgctctttat ttgggcgtag tttcactttc ttaacttgta
     2881 tcgtcttcca tcaactcctt aatcatcttc ttagctttat tggctcggtt tccttgtaaa
     2941 aggtatctac aaatagtgac aatctgtatt aaatcttcat cattttctgt aatgtcaata
     3001 atttctatat tctgtaccat atattgcaaa gggtttcact aagttcataa ccgaagcgaa
     3061 ttaaacaact tttgtataga ataactacct tttctatttt tccattggta attctatcta
     3121 ttaattggtt taatcctttt gttgtagttg attccactac caagatgatt ctctcgaatt
     3181 ttaaagcttg aagatgtatt gctatttttg ctaactggcg tatttgcaac tatttttccc
     3241 ttctattctt ccttcattga ttcccctcta aaattactat ccataaaaaa ttgcataaat
     3301 gacaatagta tttatattag tcatactgct ttttaacaca gcaaattcca gaaaaacatt
     3361 cgttacatat ttgtgaattt ttccaaaaaa taacattttt ataaaacgac gaaaaataaa
     3421 tcactaccag tcaacttcta ta
//
LOCUS       DAYYAR010000201         3324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000201 DAYYAR010000000
VERSION     DAYYAR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.205"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(499..774)
                     /locus_tag="ACHAT0_002763"
     CDS             complement(499..774)
                     /locus_tag="ACHAT0_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8452655.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            881..1645
                     /locus_tag="ACHAT0_002764"
     CDS             881..1645
                     /locus_tag="ACHAT0_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8452656.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1768..2271
                     /locus_tag="ACHAT0_002765"
     CDS             1768..2271
                     /locus_tag="ACHAT0_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8452657.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            2550..3197
                     /locus_tag="ACHAT0_002766"
     CDS             2550..3197
                     /locus_tag="ACHAT0_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8452658.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     regulatory      3239..3305
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT         1107 a          590 c          676 g          951 t
ORIGIN      
        1 tgtttctttc ttatttaaaa aactaaaaac ttttttgttc tgtctggaaa atatgggtta
       61 agcaaattca ttgcaagtta tagatcagct tttttactac ctgtctttaa tacttgaggc
      121 aaagcgcctc aaaaacaacc ttatcgacgg tgttcaaaag cttgcatcta cggcattaag
      181 tccagcctgt tgaaaaatat ggagagctat ttatcacaac cagtccttaa tgcttgatgc
      241 agaccagctt tttcacaacc ttattgacgg tgttttatca gctgcctctc ggtattaagt
      301 cattacattt caaaatatga gaagctattt tgaaaatgtt tctccttaat actcgaggca
      361 aagcgcttca aaaacaacct tatcgacggt gttcaaaagt cagtttctcg gtattaagtc
      421 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt ttccttaata ctcaaaacta
      481 agcgaccttt tcacaacctt atcccactac atcaaatttc tttcgttcct caatcatcgg
      541 atcttcttgg acatccacat actgcactcg tccacttggg ctcggagact tcttgacttc
      601 ttcgataaat ttttggatga tgtcgtcatt tccgattgct tcgattgtga ctgagccgtc
      661 gtcttcgttt tttaccgttc cggtcacgcc taactgatca gcaaccatct tggtcatata
      721 gcgaaatccc acgccctgga cacgtccttg gacattcatt ttaactttgc gcatgttcgt
      781 cacctttctt tcctgtttta taaatcaatg ataccgattt ttttaggatg aaacaagctc
      841 aagcacctat tccatgatat aatcagttga ggtgaggaat gtgaaagaga tccaatcagc
      901 aaaaaatcca agaatcaaag aattaaaaaa actacataaa aagaaataca gagaagaaaa
      961 ggcagaatat cttttggaag ggttccatct gatcgaagaa gctgcgtcgt ctaatgcaga
     1021 aataaaagaa ctttttgtga cacagagagg tatgaaagaa tggggagact ggctggctgt
     1081 aaacaaagag atttctactt ttctagtttc agatgaggtc atgaccgcta tgtcagatct
     1141 acctacaccg caagggatct tagcagtgat gacgttaaca tctgaagata cgccagattt
     1201 ttcaggaggc tggcttttac tagataatgt acaggatcct ggaaatgtgg gaacgatgat
     1261 ccgtacggca gatgcgtttg gtatggctgg tgttttatta ggtaaaggaa cggcagatat
     1321 ttatagtaca aaggtgctgc gaagcatgca gggaagcaat tatcatttgc ctgtgttaca
     1381 aaaagatttg gcagatgtaa tccctgtaat gaaaggcaaa gggtttacgg tgtacggtac
     1441 agaattaaat gaagaagcac atgcattaga caaagtagaa aaaacagagg atttcgctat
     1501 aatcatggga aacgaaggtc agggagtaag tcaggaaatc ttgtctttga ctgatcagaa
     1561 cgtatatatc ccaatgaaag gaaatgcgga atcattgaat gtcggagtcg cagcagggat
     1621 cgttctttat catttttctt tatgattaag gaactattaa gctttagcta ttttatctag
     1681 agcgactaga ataatccgaa cactggtggt atacttagat caatttcaga agaaaatgaa
     1741 attcaaaaag gtggcagatc gataattatg acagaaaaat ggcgtgaaga cgaagaatat
     1801 atgtcttatg tacaagatct tttagaaaca gaagcagtga aaaaattagc gaattacaca
     1861 cagcatgttc attccactcg tttggagcat tccatcagtg tttcttacta tagttatctg
     1921 ctcgcaaaaa aatggggtgg caatgctaaa gcaacagcgc gtgctggact tttgcatgat
     1981 ctgttctact atgattggcg tacgacgaaa tttgacgagg ggacacatgc gtatatccat
     2041 ccaagaattg ctgtaaagaa tgcagaaaaa atcaccgatt tgtcggatct tgagcgagat
     2101 atcattttga agcacatgtg gggtgcaacg atcgcaccgc ctaagtataa agaaggatat
     2161 attgtgacat tcgtggacaa atactgcgca gtcaaagaag cggctcagcc catgtctgca
     2221 agtatgcgta agagatggca acgttacttt gggaaagaat cctcgattta ataggtaaaa
     2281 ccaataataa tagactatat tccgagaaaa tctggctgat tttctcggtt ttttctgttt
     2341 gcagttgact atctgttcaa gatacggttt acttttattt atcgaatgaa tcatgtacat
     2401 agaagaatga acattaaagg gggtgatgat aagatgcaaa acgtatctta tttaaaagag
     2461 tccgggatct tcaggtaaca catactttgc agaagatcac ttttttctac aaagtatgca
     2521 ctttggttag aagaaaggac tttaaaatca tggaacaaac aataaaacca taccaatatt
     2581 tttttataaa aagagaagta gatcagttat taaatgcgta ctcatctgtc aatgatccaa
     2641 aaacggtaca aacagttcag gcactagcag ctgaaaagat tcgagacata ttagaacacg
     2701 aactgccaga aatcgataca tttttaacga cagttttaga cgtaaaactg acaaaagcgc
     2761 aagcagaaag agcattggag gagctgaagc aaatcgtcca gccgtttatg cagcctagca
     2821 aaccacaaat cgagaaatta ttccgaaaag tgaaaaaact caaacaacca gcttggaatg
     2881 aaatcgactt gcaagaacat acgtacatcg gctggaatga tccaggtacg cagaaaaaat
     2941 tcatggtcta ttacgaagat gaaaaattaa aagggattag cggtacttta tcaccaacga
     3001 taaataaggg aatctgtgca atctgtcaga aaacatcgaa tgtgtcgctt tttctttcta
     3061 caacgaaagc aggttctgat ggcacgtata cgaaaaaggg gaattatatt tgtcatgata
     3121 gtgaccaatg taatcaacag ttaacgaaat tggctccttt ctatgaattt gctgaacgtg
     3181 taaagaaaga acgataattt tattgttttt tacagaaaaa tatgctatct tttaatggaa
     3241 gtgaataggt gatggtgttc gcctttaaat gctaacgaaa gatagctaat gacgcctact
     3301 aaagtctggg gatagcccct ttct
//
LOCUS       DAYYAR010000202         3310 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000202 DAYYAR010000000
VERSION     DAYYAR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.206"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..845
                     /locus_tag="ACHAT0_002767"
     CDS             45..845
                     /locus_tag="ACHAT0_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFM8452659.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            861..1739
                     /locus_tag="ACHAT0_002768"
     CDS             861..1739
                     /locus_tag="ACHAT0_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFM8452660.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1752..2732
                     /locus_tag="ACHAT0_002769"
     CDS             1752..2732
                     /locus_tag="ACHAT0_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8452661.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            3003..3095
                     /locus_tag="ACHAT0_002770"
     CDS             3003..3095
                     /locus_tag="ACHAT0_002770"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8452662.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
BASE COUNT         1140 a          542 c          695 g          933 t
ORIGIN      
        1 gttgttaccg tagtagctac taaaaataaa gtgaggatta atcaatggaa acgattattc
       61 aaatcatgaa aaaatatgat ttttcagaga tggagacaag agtgtatacc acactattag
      121 aaaaaggtaa tcttacagga tatgaagtaa gtaaaatctc aggtgtgcca cgctcgaaag
      181 tatataatat tttggaaaaa ctattaaaaa agaatttgat tgtcgttaat aaatcagaac
      241 ctaaacttta tcatgccatc tcggcaaatg agtttttaga gaaattagaa aaaagtgtta
      301 aaaatgatct ttcttttctt accaagaatc tagggatgat caaagaaaaa gatgaagaag
      361 aaatgctgtg gaaagttgat ggtatcgagt atgttctgga taaagtagag catttggtga
      421 aaaacgcaaa agaaagtttg ctcattcaag tttggcatga gaatttcacg gattcactac
      481 taaaggcatt gcagcaagct gaaaaaagag ttgataagtt tgtactgatt ttattcagtt
      541 ctacccatga atatgatctt cccttagaaa agtattatat tcatggtttt gaaacagaca
      601 aacttgctga ctttggagca agatggatca acatcgtcgc cgatgagcaa gaagtcgtgt
      661 ttggtaccat taacgaagaa ctgcaatcaa ccgatgtcac ttggacaaaa aaccacgcca
      721 tggtcaatct agcaaaagaa tatgtcaaac atgatgcata tacactaaaa gtgattgcag
      781 aatctcctga agaacttaaa gcaaaatatg gtgagggctt tgagggaatc agaaagattt
      841 actagtagga ggaatttttt atgattcgga atattttgct gacactaatt gtcgcaatca
      901 atctatattt tattatttac tttctaagag accttttcaa aaacaaacaa aatttcaaag
      961 aagaaccagg ggatatgcgt ctgttgccct tcacgtcatt tatcatcttc tttctttcaa
     1021 catttggcgt ttccgacttt gctatcggaa ccgtccttta tccaaagttg aaatgggtca
     1081 gcatgaaaaa actacccgga acgttgaata cccaatgtgt agtaccggta gccgtcatgg
     1141 cattgtctta catcactgcc atcaacgtag ggatcaagac actagctgtt tgtattatct
     1201 gccaaattat cggtgcatat ttaggaccaa gatttgtcgt gaagttacca gagaaaacga
     1261 tcaaactgtt tgtcggcata ggattggtca ttgctgcatt gttgattttt gcaggacaaa
     1321 tgaactggat tccatcaaac ggaacagcca gtgaattgta tggcggaaaa ttaatcttgg
     1381 caggattttt actctttgtc tatggtgcat tgaacaatat cggcattggt tcttatgcgt
     1441 taacgatggt aactgtctac ttgctaggct tgaatccagt tgcagccttt cctattatga
     1501 tgggggcttg taccttctca gtaccgatcg gcagtgttca attcattaaa tttaatgaat
     1561 acagcagaaa aatcacactt tttacttcaa ccttcggtgt attgggggta ttggtcgctg
     1621 tattcctagt caaatcttta gatacctata tcctgaaatg gatcgtgatt ttggtcttac
     1681 tatacagtgc atatacgatg ttgggcagtc aattaaaaaa agttacagca gctaattaag
     1741 gagcgattca aatgttattt cttaaaaaag aagatattat caaaagtttt tcaatgagag
     1801 aagccatcga tgcggacaaa aaagcactgt cattgtattc agcaggaaag gcaagtgtgc
     1861 ctcttcgcac caatatcgat gtgccaaaaa gcaacggaca aagcttatat atgcctgctt
     1921 atgtagaagg tggcgaaggc gcgctaggtg tcaaaatcgt ttccgtgtat cctgaaaaca
     1981 tcaagaaaaa cttaccaagt gtaccagcaa cgatgatcgt attggatcca gaaacgggta
     2041 tggtatccgc ttgtttggat ggtacttatt tgacacaatt aagaacaggc gctgttcaag
     2101 gagcagcaac agaacttctt gcaagagaag atgctaaaat tggcgcgctg attggaaccg
     2161 gcggacaagc acagtcacaa ttagaagcaa tgttgaccgt tcgtaaatta gaagaagttc
     2221 ggatttttga tattgatttt gagcgtgcca gccaatttgc tgaaaaaatg atgcaacaat
     2281 tttctgtaac gatgcgccca acaaaaacca gtcaagaatg cgttgaagga gcagacatca
     2341 ttacaagtgt gaccacttca aaacgtgcga cattctctgc tgagtgggtc aaaaaaggtg
     2401 cgcacatcaa tggagtcggt gcttacacac cggaaatgtg tgaaattcca cgagaaataa
     2461 tcaaagccgc agatgttgtt atttttgata ccatggacgg tgttctaaaa gaagctgggg
     2521 acttcatttc accgctgcaa gatgggtata ttcaaagaga cagctatcat ggggaactag
     2581 gccaactgat caatgaagaa ttgatcggtc ggacaagtgg agaacaaatc acgatcttca
     2641 aaacagttgg ttcggctgta ttagatgtgg tagttgcgac agaaatcgtt aagaaagcta
     2701 aagaaaataa tttgggaaaa ttgattttct aattaaaaag gctgaaatta aaacctttat
     2761 tattgcgttt aaaaaaagaa aacaaagaag tttgtaatca aatggaaact gaccccataa
     2821 tttaaataaa ataaaaaaca tctcttgttg aattctagta atgaagcgac cctaaaactt
     2881 agaccaaaaa atctaacttt tgggtggagt tgtcaagaaa aaccgggcac ttagttaaaa
     2941 aataagttgg acgggttata ctaaaattga caccaaatta agagaaagcg aagatctgga
     3001 ttatgccaag acgttcattt gatgaaatat ttaaaattac cgctgtaaaa ctggtaacgg
     3061 aagaagggtt ttctgttaaa gaagttagtc tatagctaga agctcgctct aatagcgtgt
     3121 attgatgagt tcaagaagtt gaaaaatatg gataacgtac ttttctcggt agaggaagtc
     3181 atgaaatcca ttcgcagaaa tgaaattaca cgcttagaaa aagagaataa acggttaaaa
     3241 gaataggtag aactcctaaa aattaccgaa gtcttcctga aagaaaaggc tcttcgtcaa
     3301 attgtgttgg
//
LOCUS       DAYYAR010000203         3211 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000203 DAYYAR010000000
VERSION     DAYYAR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.207"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..345
                     /locus_tag="ACHAT0_002771"
                     /pseudo
     CDS             70..345
                     /locus_tag="ACHAT0_002771"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            372..671
                     /locus_tag="ACHAT0_002772"
     CDS             372..671
                     /locus_tag="ACHAT0_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8452663.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            845..1396
                     /locus_tag="ACHAT0_002773"
     CDS             845..1396
                     /locus_tag="ACHAT0_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8452664.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1542..1835
                     /locus_tag="ACHAT0_002774"
     CDS             1542..1835
                     /locus_tag="ACHAT0_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM8452665.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1828..2124
                     /locus_tag="ACHAT0_002775"
     CDS             1828..2124
                     /locus_tag="ACHAT0_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452666.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2199..3197
                     /locus_tag="ACHAT0_002776"
     CDS             2199..3197
                     /locus_tag="ACHAT0_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8452667.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1198 a          444 c          650 g          919 t
ORIGIN      
        1 gattgggcta tttttatggc aaaggaggga aaagaaatgt ttcaaaataa aggataacgt
       61 tatgtcacaa agggagtaat ggacagtttg ccggtggaat tacaagcttt atgttggaat
      121 ctaatcgatc aaaacgttca aaagcaattt cctttagact atcttcaaat ttttgaattt
      181 tctacggaaa aaggaaatca aaagttggtt catcgtcaag aagaaccgga agaaagaaaa
      241 gagtatctga tttcacctaa gcttcgtttg aaatcagtgt ctcaaaaaat atgggccatt
      301 gattcgggtg agtatcaaac gatgttatta ccagaagaat actagtatct aaaagaaagg
      361 aacgaaagga cttgttagaa acggctatcg aagtactgga aaagtgtgca cagctggtca
      421 ctgcttctga agagtggggc tatgaatccg ttacgatgga aaaagaggaa attgaaatgg
      481 gtatgcttcc tgaagatgtt catctaccac gtttagtgat gacacatctt tatatttatt
      541 gcgcaccaga agatggaaaa gactatgtag tttatttcat tacagatatt accagccagc
      601 gagaatttgt tcgtgggtta ctggtagaag gacgattggt ttggagtcag attggaggaa
      661 cggatgaata agatcgatta tttgagaatg attgcgaaat gtttagaaga tatccttacc
      721 gcgattcatc aaattctgaa gaaaaaagga gaaagaatat gaaatatttt caactagtat
      781 tgacacttat aacggcaatg gtaattgaag tgcttgaaca aaaaatagca taggagaaaa
      841 agagatgatt gaaaaagaat tagtgaccac acaaacagaa gtttggcgag atcctcatca
      901 tacccatcgc tatgttttca aacgacaatg ggcgatcaaa ggaaaagaag cggaagataa
      961 attagcagta gtcattacta ttcgtccaac agatacagaa ccatttacga atgatttgag
     1021 tatgttatta atcgaaaaga atatccgaca attaggattt actggtttta ttgctgtgaa
     1081 tcttttttcg tacacaaaag cgaaaagtcc tcgggtattt cttcgaggaa atgatgaaca
     1141 atctttggaa atattaacga ctgtttttac agaaaagaaa atcaatacca ttatttttgc
     1201 ttgtggttcg attacagcga cgagtcaagt ggcttatgaa caagcaaaga ctatttacga
     1261 tcaattgagt gcgaaacaaa agaagatgac caaagtattg ataaatgcag aaggtaagtt
     1321 atcccaccca ctaagtattc atgttcgtaa tgaatggcaa ttggcagata gtcaattatt
     1381 atttcaagaa aaataacatt ttattttctc atgttactta taagtagttt tacatgcttt
     1441 ttgactttaa aaatacatgt gatgatacat gttatatgaa ttatcaatgt acccagtaat
     1501 atgctatttt cattatgtaa aaaataacag ggggtgtgta gatggagatt gatgaaactt
     1561 gtttgagtaa ctcttataaa agtatgaatg acttttttga ggaaatggaa ctggattcag
     1621 aattgctcta tcaattttat cttgcttttc gagggcaaca gatttctttt ccaatgaaaa
     1681 tgtacgatcg tgagttagtt agaaaaagaa tcgaaaacat gatagaacag gaaaaaactg
     1741 tagatatccg tcaattgaca gaagtttatg gtttctctgc aagatggatt caaaaagtaa
     1801 ttaaacaaaa agaaaggaga cgagtacatg gctaagctga ttgtattagg tggtttaatt
     1861 tacggtggat attggttata tcaatatatg cttgcgcatc cgaatataga taaaggaaaa
     1921 gtaattaaga cagttggggt aatcatagtt ggactcatag ctctaactaa gaaaaataat
     1981 actaatagcc caaaaataat gagaacttat cgctgtagag gatgtggaag ggaaatttct
     2041 actagtttta atccatctga gagtcctcaa ggaagagacc gctgccatac gggctatcga
     2101 cattattggg aaagaatcga ttaaaagatt accatttaga tcaattaatt gtctgataga
     2161 ataagtacgt gagggagaaa ttgaaagggg gaaatataat gaaaaaatgg ctgttattac
     2221 taataggaat gggaatttta ttcactggtt gtacatctga gaatacgagg actaaagcga
     2281 ctatagaaac tgatacaaca attgagacta aaaaaagtaa aacgcaatca actgaacaaa
     2341 ctacaacaga aatgaagcaa tctgtggaaa caagtgcaag ttcaatcact gaatcttctt
     2401 ctgaagaggt taagaatact ttgtgggata cgaataaagc ttccaaatta gaaacttttg
     2461 tcacacaatg gggaaagaca ttaggtcaag aatataaaag ctacaatctt cagaataatg
     2521 taagtctgta tggcactcct ttaccacaag ccgtgattaa tggcgattgg aaaatggcaa
     2581 tcaatgaagc accagttacc gtacaatggt cagaagatgg aactggtcat gcagatttca
     2641 atttagtagc agtttattct gatgtagaaa ctggagaata tttagggcag catgtttact
     2701 ttttcggttt tcaaaatgga caacctaaag tttatttaac gcaacaaaat caaggtaatg
     2761 aaaataatta tctgtacttt aacgaaactc aaaatcaaca gttaaaacaa ggtttcagtg
     2821 atattgtgaa tggacaaact cctcaaacac cagtagtaga agaatctact caacaagcaa
     2881 caactacaac gacacaatct acaagtagtt caggaatttc aagttgggaa gctgctaaag
     2941 aatctgtgct aaataataaa gctttatggt tgtatgattc tcctgataaa aatgtatcaa
     3001 atactaattt aattagcaca aatgaatata tgaaagatct aatgcaagat gaaaatggaa
     3061 gctactattc aattaatatt gtaacggatg atgatggaaa cacgaccatg gctggtggaa
     3121 aacaatttcg tgtttataca gatggaagaa tttctcaacg tattggaatg actaacaatt
     3181 ttcaaatgat taaataattg aaaatattat t
//
LOCUS       DAYYAR010000204         3200 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000204 DAYYAR010000000
VERSION     DAYYAR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.208"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..786
                     /locus_tag="ACHAT0_002777"
                     /pseudo
     CDS             278..786
                     /locus_tag="ACHAT0_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            879..2041
                     /locus_tag="ACHAT0_002778"
     CDS             join(879..1155,1155..2041)
                     /locus_tag="ACHAT0_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8452668.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2321..>3200
                     /locus_tag="ACHAT0_002779"
     CDS             2321..>3200
                     /locus_tag="ACHAT0_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8452669.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQI"
BASE COUNT         1131 a          516 c          634 g          919 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaggaaata agaaaactaa acttgtatcg
      601 ttaacaaaat atggtcgaaa ggtgtacgaa tcacataatt atttacatga agagctttat
      661 agctcaataa atgaagcgtt agaatcattc tcaccagacc aaatttcaac cattaaacag
      721 attctagaaa aagtatctta tacaatcaat gaatatcaag taagtctgac aagaaaggaa
      781 gattgacatg aaaaaaatga tttttgaatc ggctacacta gactagacag aaaaaataag
      841 gtgtgtaaaa taaatgaaaa cacactggag gaatttttat gtcaagacgt caacgtagaa
      901 cctattcaaa agaattcaaa caacaagtcg tcgatcttta tctcgcagga aagccccgag
      961 ctgaaatcat tcgagattat gagctgacta cctcggcctt tgataagtgg gtcaagcaag
     1021 cgcaaactac tggctcgttt caggaaaaag ataacttgat ccctgaacaa gtagaactaa
     1081 ttgcattacg aaagaaaaat aaacagcttg aaatggagaa tgacatttta aagcaagcgg
     1141 cgctgatatt gggacgaaaa gacaagtaat tgataccaat aagcacaaat actcaatatc
     1201 agcgatgtgt aaagtgttaa acatctcttg ccaaacctat tattatcaag ccaaaccagt
     1261 tgaagatgaa tcagaactgg aagaactcgt tcaagaagaa tttattcgca atcgaaaagc
     1321 ttatggtacc aggaagctga agcagtgctt ggccaatcta ggtcttcaag tgagtcaacg
     1381 cagaatcggc cgtatgatga aacagcgtgg cttgcgttca acatacacat tggctcattt
     1441 caaattccat tcttctactt gtaacgaagc caagaccgcc aatattctag acggaacctt
     1501 cctacaggaa aagtcattag aagcgattgt aaccgacctt acctatgttc gtgttgggaa
     1561 gaaatggcag tatatctgcc tgattcttga tttgtttaat cgagaaatca tcggctattc
     1621 atgcggggag aagaaagatg cgacgttagt caaggaagct tttgcaaaga ttccgtactc
     1681 gttgaataat gtacaacttt ttcacacgga tcgtggaaaa gaattcgata accagacgat
     1741 tgacgaaatt ttatgggcct ttaatatcac gcgttcctta agcaaaaaag gctgccccta
     1801 tgataacgcc gttgcagaat ccacctataa atctgtcaaa gtagaattcg ttcatcaata
     1861 ccgctttgag acattggcta atttgcgatt agagctattt gattacgtcc attggtggaa
     1921 taccatccgg ctgcacggca cgttaggcta tgagacaccg atcagttttc gacagcagag
     1981 attggcgcag cgaatccttg ataatgagct cggatgtgat accggtggcg aggcacctta
     2041 attatttgaa tgagcttctg ccttagagaa tcagattcga ggaggctcgt tgtcaagggc
     2101 agtcggaaca ccagcagcaa gcttacgcaa taccttataa tttttgtcaa aaaaagtgtt
     2161 gccattccac atatatcttt aaacagctat tgccagataa aaataaatga tattatatat
     2221 ataaatactt atccgaaaag gggaatgaga gagtcaaggt tcttggaatg tcgaatagtc
     2281 gagtaggtag attttctatc tacaaccttg ttgtttaaaa atgaaaaaaa ttggaattag
     2341 agaagttgcg aaagaggcaa atgtttctcc tacgactgtg tcgagggtat tgaatgatcg
     2401 tggatatttg agtgaggaaa caaaaagaaa agtttttgat gccatgaaaa aattagaata
     2461 ctatcctaat gaactggcga gagcactttt taaaaacgaa acatataccg taggtttgat
     2521 ttttcctaca attactaatc catttcacgc tgagttaatt caagatatcg agttcgagct
     2581 ttccagcagg ggatataaag tgttgctgtg caatagctta aacaatccaa caaaagaaaa
     2641 agagtacttg acgatgttga gaaagaatca ggttgatgga attatagtag gaacgcacaa
     2701 taaagatatt gcggattatg atattccagg gctacctatt gtagcgattg atagaagtct
     2761 tggtgaaaat acagttacag tatcttgtga taattacagg ggtggggaat tagctactca
     2821 actacttgta aatcatggct gtcagaatat actgtgtatt cggggagata gtaagataaa
     2881 gctacccgcg aatagtagga ctttagcata taaagatgtt ttgaataaat tcatgctgga
     2941 accgttagtc ctagaagtac catttgtaaa agaaatcaat gaaaaaaatg aaataattaa
     3001 gacatatctg gaaactcatc caaatattga cggggttttt gcaggagatg atttattggc
     3061 ttctttggcg attaacaact taggaagttc gggcaaaaga gttccaaaag aggttaaagt
     3121 gattggattt gatggagctc aacaaacatt gatttataat ccagaattat caactattca
     3181 acagcctata gatcaaatat
//
LOCUS       DAYYAR010000205         3199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000205 DAYYAR010000000
VERSION     DAYYAR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.209"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACHAT0_002780"
     CDS             301..585
                     /locus_tag="ACHAT0_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452670.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACHAT0_002781"
     CDS             588..1004
                     /locus_tag="ACHAT0_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452671.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACHAT0_002782"
     CDS             complement(1141..1860)
                     /locus_tag="ACHAT0_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8452672.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACHAT0_002783"
     CDS             2522..2608
                     /locus_tag="ACHAT0_002783"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8452673.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..>3199)
                     /locus_tag="ACHAT0_002784"
     CDS             complement(2910..>3199)
                     /locus_tag="ACHAT0_002784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452674.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAYYAR010000206         3181 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000206 DAYYAR010000000
VERSION     DAYYAR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.210"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(194..343)
                     /locus_tag="ACHAT0_002785"
     CDS             complement(194..343)
                     /locus_tag="ACHAT0_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452675.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            805..1017
                     /locus_tag="ACHAT0_002786"
     CDS             805..1017
                     /locus_tag="ACHAT0_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452676.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(1295..3094)
                     /locus_tag="ACHAT0_002787"
     CDS             complement(1295..3094)
                     /locus_tag="ACHAT0_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8452677.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         1125 a          555 c          560 g          941 t
ORIGIN      
        1 taacaaaatt gtcataaaaa acgagatggt tgatattaga acttgtgttt ttgtttaggt
       61 ctagagataa agagagacaa tttccaaata tatgtaatga atacaaaaag acgtttgtcg
      121 cggattaccg ttacaaacgt cttttattga ttttccgaga tctcatcagt cgccatttgg
      181 aatggttcac tccttatcta gattcgttta atgctcggac tgcttgttcg attgtaatac
      241 caaacgcaac gttattttcg ttttgtgcat caatcgccat aaaacggtta gaagttgtat
      301 caaaagctac gcggtattgc atatttgttt ttgtaaacgt cataaaattc atctcctttg
      361 taatgtaatt tagttcttga aagtttcctt taaactttcg aacttatacc aagtttagca
      421 tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt tccttattta ttttttctta
      481 aatggtttag ctattttctt ttaatccgat aagtagccca atcatcaagg ttacaaatac
      541 tattgcttga atataaatca tatgctcacc tctttataaa taacttagca atataaagaa
      601 aaaaacgcac gaacaacata ggaatataaa caaacgtttt tgtcacaacg tctaagatcg
      661 tgttaaaaaa tagttatcta aaaagcagaa agatactcaa aataaaaaat aaaaaaatga
      721 ataaaaacgt tttcaaatac aaggtaattt gttattataa aataaagaaa aaacaaaata
      781 aaaataaaga aaagagggaa tgaaatggta aaagcagaag taggaagatc ttatatatgc
      841 aaaccgatcg gagtatctca tgaactaatc ggctgtattg aacgtacata tatcaacacg
      901 gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc aacttatcaa tgaaagcaag
      961 tatcgcatgc tcgtaaaatt ctgtgatatt agcgcaccta ctgaattggt ttcttaaaca
     1021 gtatttttga gaattcgcaa gaactccctt ataatataaa taaagacaga agggagcgag
     1081 aagatatgcc aaaaagaaaa agttcaggga aaatgggaat tattcttgca gtgatctctg
     1141 caattcctgc tattttagct attgttcgtg cgatgaagga atcaagagaa agttaagcac
     1201 acattatgag cgtcaaacaa aaaacgcata tacctgcggt ggcaggttat gcgttttttg
     1261 ttgtataaag acttttttca ttaacgattt ttgattagcc gactcccaaa acaaaattta
     1321 gcagtttatc tgaataagtg atttcttcat ctgtttgttt cacactttca tttagtagtc
     1381 ccatctcatc ggttatgata ccatccacac cataaaacat catgcgactc atggcatctg
     1441 aatcgttgat tgtccataca tatacttctt ttccgtcttg gtgggcagaa ttaatgaagt
     1501 tacggttgat cgtactatat tccattgtca gaaaatcagc aggcgtcaca gggggcccaa
     1561 caatattgaa aggtaagatg tagcccacat aaaagtttgg ttctgcttct tttaaatctg
     1621 aaacggtttg gtacgtcaaa ctttgcaaaa tatgatggtt ctctaagata actttgcggt
     1681 atttatggac aaatcgttcg acaaggtcat ccgaatcttt tttagaggtc ttgatctcaa
     1741 taagtaattt ttggtttagc ttatgtgctt tttccaaata agaatcaaaa gaacaaacag
     1801 gggcttgcac tccgttttca gtaactgtaa gatttgtcaa ttctttcaat gtaagatcct
     1861 gtggtgttcc atttacccct gttaagtgac gaagatttgc atcatgcatc actacgaaat
     1921 gaccatcttt tgtttcctga acatccatct ctacataatc aggatgatag tctctgcttg
     1981 ttttttcaag tgcttctaaa gtattttgcg ccccattttg ggtactcaca cctctgtgag
     2041 agacagtcac tggttttgtc tgaacagctg aagtcagata atccatattg taaagacaga
     2101 cgccaatacc gaaaaaaact gcaaaaagag tcaaaccagt gtttttcagc gcagagaaac
     2161 gcaagttggg ggcttgtggg ataaaccact tagggatctc aggcaagaag ccttcgtcat
     2221 ccatgaaatc aacaatgata taaaaaatcc ctacggtaga catcacgata ttgaataata
     2281 aaatcccttg taaaagcgtc atagcaaaca cagcgctgat caatgcatag tctgggaaaa
     2341 attgttcaac catactttgt gccaaaataa caacgatata tcctaaagaa gaaaggagta
     2401 aaatcgtacc gccaatcaca ataaattggc ccgtgatggc caaaagacgc gatttggtca
     2461 atgaccagct ttcccgaatg gctgctcgaa aagaacgatc tcgtagaatc atttccggta
     2521 aagcaaaaat cagtcgaatc ccaatatagc caaggaaaat ataaacgagt aaaaaggaac
     2581 tgacgatgat ccaacgatta gcaaaaataa aatccataat gaaagcgggg atttttattt
     2641 ttgacagtag atcagaatta aaacttaatc cgctgatcgg tagaatcaat aaaaaataag
     2701 caaggaaaaa caaaaaagta atgggtctca ctttcttcag ctgaagtatc gtcaagtgta
     2761 agagttgctt caaagagatt ggttctttct ttttaataaa aaaaacactc atcaaaagaa
     2821 aagtaaattc gaaataaact aacagtaaaa tcgtcatcaa aactacgatt aacgagagta
     2881 gaaccccagg atgttgagaa aaaattgaag gaatattgtc ataagacaaa tagtcaattg
     2941 cccctcggtg taaaataaaa cgggtcgtac tgctcaataa gggtaaacaa ataaataaaa
     3001 taaagccatg catcagcaag acatcacgga aataagcttg tgttcctttt aaaaagtccc
     3061 aagcattacc taagctacgt ttcaaataat tcatcctatc acctgctctt cacattgtag
     3121 cgcaaatcta atgaaaaagg aaagggtgca gtgtgttttg cagacccttt cccagattta
     3181 a
//
LOCUS       DAYYAR010000207         3068 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000207 DAYYAR010000000
VERSION     DAYYAR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.211"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            732..>3068
                     /gene="esp"
                     /locus_tag="ACHAT0_002788"
     CDS             732..>3068
                     /gene="esp"
                     /locus_tag="ACHAT0_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8452678.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGT"
BASE COUNT         1120 a          455 c          601 g          892 t
ORIGIN      
        1 ggatcagtta attgaaagag aaagctggga agattattac gtgggtctaa ctttttcaga
       61 aaagttaaac aactacaagt taatagatta taagtatttg agtgaaagca attaattatt
      121 catgtagagt actagacact ttaaatatgg acaattcaat tataaagaaa aagaatcaat
      181 aatttaatga ggctgtgaca aatgtcattt tttgtttcgc taaaaacgag tgaaaaaatg
      241 ttgataactc caatcattcg tttaagattc taggaatcat tcaggaatgc tgtgaaaccg
      301 atactttata attataaaga aggtgatttc aaattccgaa gaaggaattt gaagtcacct
      361 ttttacttat gtcacagtct cattcagtaa tgcgggataa ggctccttat tccgctattt
      421 gaatactctg aagtttaatc taagaaagag taatattttg aaaacatgct tcatcgcata
      481 gaaatgaact taatttcaga aagaatacat acatatcttt ttatatgttt tcagctgctg
      541 aatggttaaa aagcacaaat atccgaaaaa aacatgaaga aatgtacttt ttttgttgta
      601 atgtgcatga aattctttaa gtaaactagt taacataagt ttagtaagat tcctattctg
      661 tgaataatac gtatcagatt tatcttttat tcttactaag aaaataacta ttaagggagt
      721 tgatttgaat aatggttagc aagaataata agagagtatt tctagaaaaa acaaagaaac
      781 gagtattgaa gtacagtatc aaaaaattaa gtgttggggt tgcatctgtt ttggttggtg
      841 ttggtcttgt cttgggaaca actgaactag ttcaagcaca agatgaaatt tctccaagca
      901 ccccacttga aacagcgata agctccgtac aagtaggtga caaagtagct tcggggaata
      961 cttttcagga gaatcctggt tatacgaaaa attataactt ttcggattta caatttagtc
     1021 ctcaagaact aactggagac acactaaaag gcaatacaat tggatttgaa gtttacggaa
     1081 aacataatat tgcagcatca actaaaaact gggaaatccg tcttcaatta gatgaacgat
     1141 tagctaaata tgtagaaaaa attcaggtcg atccaaagaa aggaattgga tcttctagac
     1201 ggacctttgt aagaattaat gattcgctgg gtagacctac aaatatttgg aaggtcaatt
     1261 atattcgtgc aagtgatgga ttatttgctg gggctgaaac aactgataca caaactgctc
     1321 ctaatggggt aattacgttt gaaaagagct tagatgaaat ttttaaagag ataggcatag
     1381 acaatctcaa gaccgatcgt ttaatgtatc gaatctactt ggtaagtcat caagacgatg
     1441 ataaaattgt accaggtatt gatagtacgg gctacttttt aacagattca gatgatttct
     1501 ataatagctt agatgtatct gaaaataatc ctgaccagtt taaacatgga tcagtaagtg
     1561 caaaatatga ggaacctaat actcaaacaa aagatggttc aggatccaca ggtgctaatg
     1621 gagctataat cttagatcat aagttaacga aaaattataa tttctcatat tcagcttccg
     1681 caaagggcac gccttggtat gctaattaca aaattgatga acgattagtt ccatatgtag
     1741 cgggtataca gatgcacatg gttcaggctg acaaagtaac atatgatgtt tcttttgaat
     1801 cagggaaaaa agtggctgat ttagcaattg aacggcgcaa agatcatgaa aattatggtg
     1861 taggttcaat tactgataat gatcttacca aacttattga ttttgctaat gcaagtccac
     1921 gtccagtcgt tattcgatat gttttacaat tgactaagcc attagatgaa atcttagaag
     1981 atatgaaagc aacagcacaa gttgaagaaa ataaaccatt tggtgaagat ttcatctttg
     2041 attcttggtt gtcggatacg aataaaaaat taatccagaa cacttatgga acaggttatt
     2101 attatttgca agatattgat ggtgatggaa accctgacga taaagaagag agcggagaca
     2161 cgaatccata tatcgggaaa cctgaattag aagaagtata tgatgttgac acaacagtta
     2221 aggggaaagt attcatccac gagttagcgg gaacaggtca caaagcccaa cttgttgata
     2281 aagaaggtac tgtattagca gaaaaaacta tcgctccaaa tgaaaaagat ggggctccaa
     2341 tttcagatac tgtagaattt gaatttacgg gtgtagattc aagtaaatta atcgcgaaag
     2401 atgaattaaa aatccaaatc gtttctccag gttttgataa accagaagaa ggttcaaccg
     2461 ttattaagga atcaccaaaa gcggttgata aacaaaccgt ggtagttgga tttaaaccag
     2521 atgctaaaga atcaattcgg aataataaaa acttacctga agatgcagag tattcatgga
     2581 aaacagagcc tgatacttct aacgttactg atagtacgaa aggtattgta actgttaaga
     2641 tcggaaatcg aactttcgac gtggatgtag agtttgctgt aaaagcttct caagctatgg
     2701 aaaatgatgc aacatacgta cctataacaa caactccaga aacgacagtt caaagtggaa
     2761 aacctacatt tgataaacca gatgttcctt tagctaaaga tgccttttca attttagatg
     2821 tctataataa ggacttcggt aatgcaagtg ttgatgcaaa tactggtatt gttacattca
     2881 ctccagctaa aggtgtagga gaatcggagc cgattactgg aacaattcct attaaaattg
     2941 tttaccaaga tggttctgta ggcacgaccg atttagcagt aactgtaagt aaagatattt
     3001 atgaaaatcc aggagaaaac attcctgcag gctaccacaa agtgaccttc accgcagggg
     3061 aaggaaca
//
LOCUS       DAYYAR010000208         3044 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000208 DAYYAR010000000
VERSION     DAYYAR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.212"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..769
                     /gene="fabI"
                     /locus_tag="ACHAT0_002789"
     CDS             14..769
                     /gene="fabI"
                     /locus_tag="ACHAT0_002789"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8452679.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(879..1931)
                     /locus_tag="ACHAT0_002790"
     CDS             complement(879..1931)
                     /locus_tag="ACHAT0_002790"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8452680.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2057..2977
                     /locus_tag="ACHAT0_002791"
     CDS             2057..2977
                     /locus_tag="ACHAT0_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8452681.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          883 a          616 c          587 g          958 t
ORIGIN      
        1 aaggagagtc attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa
       61 caaaaaaagc attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat
      121 ctatacttac caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc
      181 attcactgta gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat
      241 cggtgaatat gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga
      301 agaattgtcc ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat
      361 cagcagctac tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag
      421 cgggatcgtt agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat
      481 gggtattgcg aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc
      541 taaaggaatt cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg
      601 tgttaaagac tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg
      661 tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg
      721 aatcatcggt gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc
      781 attctttaaa gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt
      841 ttttcttttt acgttttttt caactcatta ctttgaaatt attccacgac ttcaatccag
      901 ccttctggtg cttctatgtc tccaaattga atacctgtca attcatcata caaccgtttt
      961 gttactggtc ctacttccgt ttcactataa aatacatgaa gattgttatt gtgataaatc
     1021 ccaccgattg gagaaataac agccgctgta ccacatgcac cagcttctgc aaattgatct
     1081 agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc ttcagctaac
     1141 tgcaacaagg aatatttcgt gatacttggt aagatagagg gcgattgcgg agtgatgaat
     1201 tttccgtctt ttgtaattcc aaagaaattt gctgcaccta cttcttcgat tttcgtatgt
     1261 gtggctggat caagataaac acaatctgag taatccagtt cttttgcttc tgttcctgga
     1321 agtaaactcg ctgcgtaatt tccgccgact tttgctgcac cagtcccctt aggagcagca
     1381 cgatcgtaat cagatacaat aaaattcgtt ggttttagtc cacctttgaa atacgctcct
     1441 actggcgtac agaagacgac aaataaatat tctggagctg gatggacacc aatgttttca
     1501 ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata gggcggcaca
     1561 aaatcttcat ttgcttttac cacttgcttg acagcatcaa cgaacatctt ttcagggacc
     1621 attggcatca gcaatcgtct agcactttga ttcatccgtc gactattttg ttcaggtcga
     1681 aagagattga ccttgccgtc ttttcgacga taagctttta gaccttcaaa acattgctga
     1741 ccataatgaa gcgctggaga gccttcatga atcgtcaggt aattatcttc tgtcaattct
     1801 ccttcttccc attgtccgtc tttccatttg gcaataaaac gccaaggagt tttgatataa
     1861 gaaaaaccta gattgttcca atcgatttgc atatacttac caccttcgtt attttctcca
     1921 cattttatca ttatccatat tatttgtcat tttattttca gcttttttcc ttcattatag
     1981 ttatttttgc tatttccttt tttgaaaact tcttggtaca atacagaaag aatcaaattt
     2041 tagaaaagag gattttatgt tttcattatt agcaactgcc ggtacgacag actcttctgg
     2101 cgagacattg acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt tttgggacgg
     2161 gattgattgg gacgccattt tggctgcctt tatccaaaaa agtttatatc tgattttttt
     2221 gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat ctgattgata agtcttttaa
     2281 tcagtattct aaaaaagcca ttctaaacga gagccgctta aagactttac atagtctttt
     2341 aaatactatt tttcattata cccttggttt cttttttatc tatgcaattt tatctgctat
     2401 cggtgtgccg atcggatcac ttttggctgg tgccggaatt gcaggtgtgg caatcggtct
     2461 tggtgcccaa gggttcatga gtgatgttat taccggattt tttattatca tggaacaaca
     2521 gatggatgtt ggggactaca tcaagctagc aaacttaacg attgaaggaa ccgtcgtatc
     2581 tgttggtatc cgtacccttc agctgaaagc aacggatggt actgtccatt tcatcccgaa
     2641 tcgaaacatc accacgatca gcaatctctc tcgtgcaaat atgcaagtac ttttagatat
     2701 tcggattgtt cctgaagaag gatatgacaa aatctatgag atcattgatc gagtaaatcg
     2761 tacattagct gaaaaatacc aagaagagct ccaaactgaa ccttctatct ttggattagt
     2821 ggatcttgga aacagcaatt ttgcgattcg tacggtctgc tacgcattga acggaaaaca
     2881 atatgcctta aaagaagagt tcttgagctc ctatgtcaaa gaattgacag cttctggttt
     2941 cacgattccg aacagtccaa ttgctgtcgg aaaataattt ggacaaacga tgcaacgaat
     3001 ctcactaaaa agagcgtggg acaaaagtaa agaacacttt tgtc
//
LOCUS       DAYYAR010000209         3038 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000209 DAYYAR010000000
VERSION     DAYYAR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.213"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACHAT0_002792"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACHAT0_002792"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8452682.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACHAT0_002793"
     CDS             complement(1034..1954)
                     /locus_tag="ACHAT0_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8452683.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACHAT0_002794"
     CDS             2067..2819
                     /locus_tag="ACHAT0_002794"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8452684.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1044 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagat
//
LOCUS       DAYYAR010000210         3029 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000210 DAYYAR010000000
VERSION     DAYYAR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.214"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            184..2418
                     /locus_tag="ACHAT0_002795"
     CDS             184..2418
                     /locus_tag="ACHAT0_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8452685.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            2566..2886
                     /locus_tag="ACHAT0_002796"
     CDS             2566..2886
                     /locus_tag="ACHAT0_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8452686.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         1077 a          561 c          611 g          780 t
ORIGIN      
        1 aattacttgc ttcccttttt atttttagta taataagagt caaaggtcag gaatggtcaa
       61 tagagatttc accttttcta cctcataaat ctcttaaaaa actaggggtt attcttttac
      121 ctcatgaaaa acgagatgta aaatatggat gtacccattt ttgtgaaagg acgtgaatga
      181 ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga ttcacttgta tgccactgtc
      241 aatggtcagc ggacacaact cgattactgt cagagctgtt atcaaaaaat caaaaatcaa
      301 caaaatggag gtctgactaa tatggcccaa aatgatccat ttggatttgg cagtttggac
      361 gatcttttcc gttccatgtc tcgccaaatg caacagcaag gaaattatga gcaaacacca
      421 ccaacacaat ttggcggaaa caacaacttc aatggtggtc agccacctca aggcaataca
      481 gaaggtttat tgggagaata cggaatcaat atcacagaac aagcacgaca aggtgatatc
      541 gatcctgtga tcggacgcga tgacgaaatc aaacgagtca ttgaaatttt aaatcgccga
      601 accaagaaca atcctgtctt gattggagaa ccaggtgttg gtaaaacagc agttgttgaa
      661 ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa aattaatgga caaagaagtc
      721 attcgtttag atgtcgtatc tttagtccaa ggtactggta tacgaggaca atttgaagaa
      781 cgtatgcaaa aattgattga agagatccgt caagcagaaa atgtgatcct attcatagat
      841 gaagttcacg aaatcgtcgg tgctggttct gctggtgatg gaaatatgga tgctggtaat
      901 attttaaaac cagctttagc tcgaggagaa ttgcaaatgg ttggtgctac taccttaaat
      961 gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc gtatgcaacc tgtacgggta
     1021 gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag gtttgcaaaa acgttatgaa
     1081 gactatcatc atgtaaaata tactgatgag gccatcgaag cagccgcaac tttatccaat
     1141 cgttacgtcc aagatcgctt cttgccggat aaagccatcg atttgctaga tgaaacaggt
     1201 tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa caatcgagaa aaaactgcaa
     1261 gaagcagaag aacaaaaagt tttagcttct cgagaagaag atttcgaaaa agcagcttat
     1321 tatcgcgatc agatcaataa acttcaaaaa atgaaagaac gtcagctcac tgaagaggaa
     1381 acacctgtga ttaccgaaaa agatatggaa aaaatcgttg aacaacggac aggtattcca
     1441 gtgggtgaat taaaagaaaa agaacagaca caactgaaaa acttagcaga tgacttgaaa
     1501 gctcatgtca tcggacaaga taacgctgta gatcgagtag cgaaagcgat tcgccgaaac
     1561 cgtgttggat taaacaaaca gaatcgtcca atcggctcct tcttatttgt tggaccaact
     1621 ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg aactattcgg ttcacaagat
     1681 tcaatgattc gtttcgatat gtctgaatat atggaaaaac atagtgtctc taaattgatt
     1741 ggttctcctc caggttatgt cggatacgaa gaagctggtc aattaacaga aaaagtacgg
     1801 agaaatcctt acagtcttgt cttgttagat gaagtagaaa aagctcatcc agatgttttg
     1861 catatgttct tgcaaatctt agatgatggt cgattgacag atgcacaagg aagaacggtc
     1921 agctttaaag atacgatcat cattatgaca agtaatgccg gaactggtaa agtagaagca
     1981 aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt ctgtattgaa ccaattaaac
     2041 aactacttta caccagaatt tctgaaccgt tttgacggga tcatcgaatt cagtgcatta
     2101 tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag acgatgtcaa ccaaatgctg
     2161 gcagcacagc aacttcacat cgaagttcct acgaatgtca aagaaaaatt agttgactta
     2221 ggctatgatc catctatggg tgctcgtcct cttcgacgca cgatccaaga acaaatcgaa
     2281 gatggtatcg ctgaatttta tcttgatcac ccttctatcc atgagttgaa agcaaaactg
     2341 gataaagatg gaaaaatcat cgttacttct aaaccagaac gtttggctaa agaatcagcc
     2401 gaagaaacgg ctgaataaat ccagttttat agcgtgagac ggtatttctt tgagaaatgc
     2461 cgtctcattc ttttttcaga aaagtcaaaa tactgttcat tttataaaaa agaaattata
     2521 ataagagaaa caagcaatta aaaatacata aaagaggtga gcgccatgaa attggtaaat
     2581 gtaacaaaca gccattcccg tcttgtatta aaccagcttg aaaacactga tgcccatcta
     2641 gtcaaggtct acacagccgg aaataccact attgtctaca ctgaagcccc tgaacataat
     2701 gaaattctgc tgatcaatga caaacgtaaa atccagccga aagaaatcga agatgctaaa
     2761 aattatttct tagataaaat caaagacgcc gtttaccacg aggatgaaat caaagtaatc
     2821 gaattggcag gccttgttga aatctcgatt ccaaaacatg aattaggcgc tacattagcc
     2881 gtgtaaacaa attttgaaca taataattaa agagcgtggg acaaaagtaa agaacacttt
     2941 tgtctcacgc tctaaacacg tataaacggc gaaagcagaa gcaactcctt cggaaataag
     3001 ctgaaattca cgaaaattgc ttttcaaat
//
LOCUS       DAYYAR010000211         3014 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000211 DAYYAR010000000
VERSION     DAYYAR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.215"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1469)
                     /locus_tag="ACHAT0_002797"
     CDS             complement(159..1469)
                     /locus_tag="ACHAT0_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8452687.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="ACHAT0_002798"
     CDS             complement(1481..2899)
                     /locus_tag="ACHAT0_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8452688.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          945 a          622 c          467 g          980 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaa
//
LOCUS       DAYYAR010000212         3001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000212 DAYYAR010000000
VERSION     DAYYAR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.216"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACHAT0_002799"
     CDS             <1..963
                     /locus_tag="ACHAT0_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8452689.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="ACHAT0_002800"
     tRNA            1152..1225
                     /locus_tag="ACHAT0_002800"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
     gene            complement(1740..2039)
                     /locus_tag="ACHAT0_002801"
     CDS             complement(1740..2039)
                     /locus_tag="ACHAT0_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8452690.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2045..2275)
                     /locus_tag="ACHAT0_002802"
     CDS             complement(2045..2275)
                     /locus_tag="ACHAT0_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452691.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(2534..2647)
                     /locus_tag="ACHAT0_002803"
                     /pseudo
     CDS             complement(2534..2647)
                     /locus_tag="ACHAT0_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1019 a          509 c          519 g          954 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
     1261 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
     1321 ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata tttctgtata
     1381 tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttatat tgtgagataa
     1441 taataaagaa gagtttaaag cgttccccaa aaaccactgc cccataagtg tgttacgctt
     1501 taaactcttt tatatttgaa gccattaaaa agcataccat atttttgaaa aaaagtgaga
     1561 aaaaaggctt ataattggag tggtagttaa ttagtgactt atttttgatt ttatagcact
     1621 gatactataa aatatagata tcatcatatt acacaatctt aatactaact taaaaaatat
     1681 ctcctttcat aagtatggtg ataaaatccg ttccgggcta cctttttagg tagcctactt
     1741 taatctttgt atctttctgg atcaacgaaa gtatacttta tatagtcata acgccgatga
     1801 tcgcttcgag cgtctagcac atcagtcacg atatcaaaca aaaaatatac gtctttcttc
     1861 attctagttt tcgcagcagg gattttaaag tagttcttat tagaatagta gagattaatt
     1921 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
     1981 ttgacaaatc tattcgaagg atcaaattta aaccataatt gcgtctcatt aaaaagcata
     2041 accattactc cgatctattt ttaaacttag tctctacctc taatatatat cgagttttta
     2101 ttttgccttc agagaatact gtttcttttt ttgcagttac aggttgttta ttttcggaaa
     2161 aagctaatat agctaaaatt gaaacatcca tctggaattt gtctttttcg ttgctttgct
     2221 cataaaaatc tgcatattca tcactaatat tttttctaat aaattcttcc atcataaaga
     2281 tcacctcgaa aagagtatac gaacaaacgt tctttttgta aaggtggaat ttattatact
     2341 aaataaatag gtgaacaagt acttgtgcca aattatgtgc caaaaaaaat cgaatttaat
     2401 aaaacacaaa caaaaagaaa tcctattatg ataacatttc ttataataac aaacacaaca
     2461 gaagacgtgt aatagttagt caggaacgta caaataaccc ctgtatcctt tgcggtacag
     2521 ggggttattt tgtataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg
     2581 atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc
     2641 catcaataaa cagtcgaaga gaaaataggg aaaaattgat tactaaagaa aaacttacta
     2701 ggtcgacggt gtagttgatt ttcatacttc aatagattcg aattaaacga tttcgattgg
     2761 cttataccag tcctatccaa cgaaagatat atcgttctta actctagcgg tataacttga
     2821 agctttacgt aaagccaatg ggaatgaact ggagatgact ttttattatt tttagtaata
     2881 gctgatgggt taatacttac aactttcctt ttcaaagtac catataaaaa aacggtgggg
     2941 ttgtttagcg aaataaagga tctaagccaa tagcttagat cctttattct atgctttata
     3001 t
//
LOCUS       DAYYAR010000213         2942 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000213 DAYYAR010000000
VERSION     DAYYAR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.217"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..752
                     /locus_tag="ACHAT0_002804"
     CDS             144..752
                     /locus_tag="ACHAT0_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8452692.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            752..1093
                     /locus_tag="ACHAT0_002805"
     CDS             752..1093
                     /locus_tag="ACHAT0_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452693.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            1335..>2942
                     /locus_tag="ACHAT0_002806"
     CDS             1335..>2942
                     /locus_tag="ACHAT0_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8452694.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNA"
BASE COUNT         1017 a          396 c          644 g          885 t
ORIGIN      
        1 gttgtgggac tttttttaat acgattacaa tcgatgagca aatattattt ccaaaagcct
       61 atttacctga tgaagcagat ggtgttcaac atattcgttt tacattagaa tttccacaat
      121 atattgaatg gagtgaaaga taaatgggag aaaaaagaag taaaaccgga attatttctg
      181 tcgaaaaacc tacttggttt cctttggaag atgagacagg gaattttccg gtatatggta
      241 ctgcgttgcc aatgggaaca gcagtaagta tcaaaccaac agctaattat gaaacaacac
      301 aagattatgg ggattcagtg gttcaagatc aatttacagc ttttggtggt gcagaagttg
      361 aactggaagc aaatggatat actcataaag ttttatctac aattactgga gggaaaattg
      421 ttaaaggtgg tgccttgcgt tctggagaag atattgcgca agatggtgcg tttgcatata
      481 gacgtaaaaa atcgaatggg aaataccgtt atacggtctt ttataaagga cagtttgctc
      541 tagattcaga tgaaacatca acgattgaag gaagtaaagt aagttttact catccaactt
      601 ggaagggttc atttgtagat gtaccaggac ttggatatat gtattcagtt gatgaagatg
      661 atgaaggcgt ggataaagca atgatcggaa attggtttac aaaagtagca attccaattg
      721 aagaagcaga actttcagga ggtacagaat aatgtcaaaa tatcaaacaa cgattaaatt
      781 aacgaaaaaa gacgaagaag gtaaatatga gcaagtacaa tttaaatctg ctgaattttt
      841 accaggaaca gttgtagaag atgctgcagg agttatggaa gaaatgcaaa cagcgactga
      901 taagcaatca gttaaaaaag ctttaagtcg tgcttattca tttattgcag atactttatt
      961 tgagggacaa tttactggtg aagattattg caaaggaatt gatgctcgtg agattgcctc
     1021 attgacagga aaattattga agtctgttac tgcaggtttt gatgaaactt atacagagac
     1081 gaaaaaaaag taagtgaggc gctcaagtca ccttcattta agtattcgat tacttaccga
     1141 gaattagata taaagacgca attactcgaa gcaggttgga cgttaccaga aattgaacgt
     1201 actgacttgg atgagttaat gcgtcttttt gcttttagag atgcggtaaa agagcatgaa
     1261 gatgttgagt actatgataa tttcactcaa ttttaggagg tgatactttg aataacgatg
     1321 acctaattct gaagatgata ctagatgaat ctggttttac tgccggtatg aataatgctg
     1381 tcaaaaagtt gaactctttt gatgaaacaa tcgaaagaaa aagtagaaat agtggcagtt
     1441 cattaggtag tatttggaaa atatttgctg gaagttttct agctagcgga gtaactagaa
     1501 tcgtaggagc tggctttgat ctaatcaagg gttccataag tggagcggtt gatcgagtag
     1561 atacgatgaa taacgctcta cgaaatttcc aaaatatggg atttagtaat tcagaaatta
     1621 tgaagaatat cggaaagaat gggcttttat ctcaaggtat tcaaggactt cctactgctt
     1681 tgaatgatgc gataagtcat gttcaacttc ttgcttcttc tacaggtgat ttaactcgtt
     1741 caactcaaat atttaaagct ttaaatgatg gaattcttgg tttcggtggt tcaactgatc
     1801 aagttaatga agctgttatt caattatctc aaagtttctc aaacggaaaa gtagacgcac
     1861 aaacttggaa ctcaatgatc aacgctcaac ttggacctac tctttctgct attgctaaaa
     1921 agatgggaat tacaatggga gatctgaaag aaggtttatc tcaaggtaag atttctgttg
     1981 aagaattcca aaatcaatta atagaaatgg ataccaaagg tggcggagga cttaaatcat
     2041 taagtcaaat cgctaaagat tctactaagg gaattaaaac ctctatacaa aatgcaaaaa
     2101 cagctgttac acgtggtgtt ggtgaggtaa tagaaggatt aaataaagca ttagtagatt
     2161 cagatttagg tggatttaag ggaattattg ataaagtggg cagttcaatg gaatcgttct
     2221 taaaagtaat tgctgcaaat attcctacag cagtatcatt tttaagtaat ttatttaacg
     2281 aaattcaaaa atttggttct gctttgaaat tcatgatgcc ctttcttgtt cctgcagcta
     2341 ccgcttttgg agcgttcatg tttcaactta aaggtatacc agcaataata aaaagcttca
     2401 ataattttaa gaatgccata attggcgttg gaaattcatt aaagattatg ggggcaatag
     2461 ctgctgctaa tccatttgtt ttaattattg gtgcagtcgt tggtgcaata gctgttttcg
     2521 gttactttat ggcaaccaat gaagagctta gaaacaaggt tatatctatt tggaatgatg
     2581 tgaaagattc cgtaattggt gtattaaaaa atataaagga ctggggaatt gatacttgga
     2641 attctgctaa agaaatggca tcaaatgcag ttgagggtgt caaagatgct tggtcaggaa
     2701 caaaggaatg gttttctaac acctggaaag gtattaagga tggggccaca ggtttatttg
     2761 ataaaacagt agagacttca agaaatgcag tcgatagtgt aaaaaatgca tggtctaata
     2821 tgaagaaatg gttttctgat acttggcaaa gtataaaaga ttcagccaga gaaaaatgga
     2881 atggaatcaa aggttctatt atgtcagttg ctggtccatt gattacaggt ataaagaatg
     2941 ca
//
LOCUS       DAYYAR010000214         2934 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000214 DAYYAR010000000
VERSION     DAYYAR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.218"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACHAT0_002807"
     CDS             14..1141
                     /locus_tag="ACHAT0_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8452695.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1348..2490)
                     /locus_tag="ACHAT0_002808"
     CDS             complement(1348..2490)
                     /locus_tag="ACHAT0_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8452696.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2495..2680)
                     /locus_tag="ACHAT0_002809"
     CDS             complement(2495..2680)
                     /locus_tag="ACHAT0_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8452697.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          972 a          534 c          458 g          970 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaatccatg taaacatggt ttatttttta
     1201 cactgttttc aaacaaaccc ttgctatttc ttcggttcgt cctcgtccat tttcgatacc
     1261 gacagctagc ttataaccca aggtatatca acataaaaaa ataaaaatag gctatgacag
     1321 cagcgtgaca gcaaaaaaga tggaaagtta aatatcaaac ctcgtaattt catttgcgag
     1381 tgcttgattt gtttctggat ataagtgtcc atatttgtcc aaagtagttt taatagaggc
     1441 atgacctaga cgcttagata ttgcatagat atttttatta tgttcaatta aaaaggcaac
     1501 atgcgagtgt cttaatccat gcatatgaat tggttttact cctgcaattt cagctagttt
     1561 ttttattctg cttaaaatag ttcttggaga tggcggtaat ccatcaaaag aaaaaaccaa
     1621 ttctatattt ttcccaattg attgttgagt ttttttccat gtttttaaat cttcaacggt
     1681 ttgtctgtct aacgaaatta tccgaatact tgatgtattt tttgtttcag caaattcata
     1741 atcttgtctt gtacggatgt aaattgactt atttacgcta catctgccat ttattaagtc
     1801 aaagtctttc cattgtaacg ctaagagctc ctcactcctt acacctgtaa caaaaataaa
     1861 acgcatcaaa cgtttataaa attcttcata aaaatccccg tgataaacac tattatatac
     1921 tttcttaaat tcatcaactg tccaaaattc tacttgagga cgctgagttt ttattttacc
     1981 tatagtatct acaggatttt cttcaataat ttcaagtaca attgcccttt taaaaactac
     2041 tctcaactta ttgaatatct gtttaatata atttggtgat aatggttgct caatgccctt
     2101 ttttacaaca tggattttcg ataaaaactg ttgaaattcc tgtatatgaa ttggtcgaat
     2161 ctttacaacc tgcatttctc caaaaaaatc caatgcacgt tgtaaaatat gatctgtttt
     2221 catatatgtt ttttctacag ttcccaactt ataccaagga aaaaaatact ctcgataaaa
     2281 atcatgaaac gttattcctt gcagataaat tccttgtgaa tcagaaaaac tttgttttaa
     2341 ctcttcgagt ccccttctag cttctccttg agttttgaag cctcttctgg tcacttgaat
     2401 tctttttcca aagcgatctt gtcctaaagt tgcagaaaaa aaccaagtgc ctctttttct
     2461 gtctttataa acattcgcaa ttttcctcat tttactattc ctcccaagaa atatggagaa
     2521 cttcttctaa aattgaaata ggaattcttt ttatttttga attacgataa aaaaagtatc
     2581 ctttctcaac taaaagttct ttacattcct tgaagatttt attagcatag tactgagaaa
     2641 aacctagatt aatcaaatct tttcgagtag ctgttttcat tatagcacct tccttacttt
     2701 ttttcattta tttctatcag ctgcttttgt caaaagcaca taggaatttc acctctcccc
     2761 aagtttacgg cgagccttac ggaagtatca ttatcccaac tttaaatcat cgctttttat
     2821 tgactataaa aattatttta gaatatttat cgctccttct ttacagcaag aaatcatggc
     2881 gcaactaaaa tatgctcctt aaagtgttaa cgtactgata gaatctattt aatt
//
LOCUS       DAYYAR010000215         2932 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000215 DAYYAR010000000
VERSION     DAYYAR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.219"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..1512
                     /locus_tag="ACHAT0_002810"
     CDS             34..1512
                     /locus_tag="ACHAT0_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM8452698.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(1580..2671)
                     /locus_tag="ACHAT0_002811"
     CDS             complement(1580..2671)
                     /locus_tag="ACHAT0_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8452699.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT         1030 a          523 c          461 g          918 t
ORIGIN      
        1 tgttattatt agcaacaaaa aaaggagtgc atcatgaaaa agttatctca aaaacgttta
       61 gctcaaatca ttattgattc tagaaaatct ctgaaaatga cacaacaaca acttgcagta
      121 gctactggta tcaaccgtgc cttaattagt cgattagaat ctctcgattt tatcccgtca
      181 atacaacaat tagaatcatt agcagaagtt ctaaattttg aaccaacaga tctatttata
      241 aatgaatcaa ataaatcaaa cagaacaaaa catcagtcga agaatattgc tgtagccggt
      301 actggttacg taggtctttc cattgctact cttcttgccc agcacaatca ggtaacagca
      361 gtagacattc ttcctgaaaa agtaaaacaa attaacaata agaaatctcc aatacaagac
      421 gattacattg aaaaatatct agctgaaaaa aatcttaatt taattgcaac gactgatggt
      481 gcagccgcct atagagaagc cgattttgtt gtcattgctg ctccaacaaa ttatgatagt
      541 aaacaaaatt attttgatac ttctgctgta gagtcagtaa tcaaattggt gttagaagtc
      601 aatccaaagg caataatggt catcaagtcg acggtacccg taggctatac cgaaagtatt
      661 cgtaaaaaat acaatactaa aaatattcta ttcagcccag aatttctcag agaatctaag
      721 gcattatatg acaatctata tccttcgcga attattgtcg gcacggacca aactgacaaa
      781 actttacgag aagcctctca cacttttgca gatttactcc aagaaggagc aatcaaaaaa
      841 gatatcgaaa ctcttttcat gggattcaca gaagctgaag cagttaaatt attcgctaat
      901 acttaccttg cacttcgagt atcttacttc aatgagctgg atacttatgc agaaatgaaa
      961 ggactaaata cacaagaaat cattcacggg gtttgcttag atccaagaat tggcacacat
     1021 tataataatc catcttttgg atatggaggc tattgtttac cgaaagatac aaaacaactt
     1081 ctcactaact ataacgatgt tcctcagaat atgataagtg caattgtcga tagtaatcgt
     1141 acaagaaaag actttatagc agatcgagtg ttagaacttg ctggcacgta tgaggctaac
     1201 agttcgtgga atgcagaaaa agaaaaagag gtagtcatag gtgtataccg tcttacaatg
     1261 aagagtaatt cagacaactt ccgtcagtca tctattcaag gagttatgaa gcgaattaaa
     1321 gcaaaggggg caactattgt aatttatgaa ccaacacttg aaaataacac tacatttttt
     1381 ggttcaaaag taataaataa tttagatgat tttaaaaata tcagtcacag tattattgct
     1441 aatcgttacg atagtaatct tgatgatgta aaagaaaaag tatataccag agatttattt
     1501 caacgagatt aaaaaataac acctcagatt tgtagtgtct gaggtgtttc tataaacttt
     1561 tataagtaaa aaaatacaat tatacttcat aaaaagactt ataccactgc gcaaatttac
     1621 gaagcccgtc acgcaaactt gtacttggtt taaattcaaa atctcgttct aacgcactag
     1681 tatctgcata agtaactggc acatctcctg gttgcattgg aactagttcg gtatgagatt
     1741 caaagtcgta atcttctggt agcacccctg ctgcaattag ctcttgttgt agaatatcta
     1801 caaaatctag taggttttcc ggcgtattgt taccaatatt ataaatatga tatggtggga
     1861 ttggtagccc atcttcccca tcaattttct ctggagcgca ctgcataacc ctctttactc
     1921 cttcaacaat atcatctata taggtaaagt cacgcttgca attcccataa ttgaatattt
     1981 ggatattatc accagcacga agtttatcag taaaaccaaa ataagccata tctggacgcc
     2041 cagcaggacc atatactgta aagaaacgta accctgtaga cggaatattg taaagtttag
     2101 aatatgcatg tgccattgat tcattacttt tcttagttgc tgcatacaac gatacttgat
     2161 tgtctacttt atcctctgta gaataaggaa ttttcttatt agtaccataa acggaagacg
     2221 atgaggcata cactaaatgt tccacaccag attctccatt atcatatgaa tgacgacaag
     2281 cttcaagaat attatagaat cctattatgt tagattcgat atacatatct ggattagtaa
     2341 ttgaataacg gactcctgcc tgagctgcta aattcaccac gatacttggt ttataattat
     2401 caaagatgaa atcaattgtt gctttatcag caatagaacc tttgataaaa ttccaagtag
     2461 atcctggatg gttctttgcc tccgtttcta tctctgataa tctatagttc ttaatagaga
     2521 catcatagta atcattcata ttgtcaatgc caatgatttt tattgatgac tgagtacgta
     2581 ataactcaag aaccaaattt gatccaataa aaccggctgc gccagttatt aatattgttc
     2641 tatttgataa ggacgtgttt aattccagca ttttttagca ctcactttct ttttttctta
     2701 tgaatacata tttgttatat attaaaaaat cttatttaga cttatctatt gtcactttta
     2761 tcattctaat ctttgcacgt agtagaataa ttatacagat aggaaattgc taagcaaccc
     2821 acttctttct attatctatt aacttattct aaaacgaata gaaattattc ttgatcacat
     2881 aagtaacctt aaaagaaaaa atttaatata tgtgtctaat tttggcaaat at
//
LOCUS       DAYYAR010000216         2922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000216 DAYYAR010000000
VERSION     DAYYAR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.220"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1368
                     /locus_tag="ACHAT0_002812"
     CDS             <1..1368
                     /locus_tag="ACHAT0_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452700.1"
                     /translation="SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIAL
                     TIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSK
                     QIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDS
                     IIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFL
                     FQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIA
                     AKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGE
                     SWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVD
                     VNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINK
                     KNENK"
     gene            1355..>2922
                     /locus_tag="ACHAT0_002813"
     CDS             1355..>2922
                     /locus_tag="ACHAT0_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8452701.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTI"
BASE COUNT          997 a          435 c          503 g          987 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgatattttt caaaaataga agatacaata agacaagaga
     2461 actattttat ttatttatga ctgttttgtt attaatcact ttaactgttt ctagtaaaaa
     2521 aaatttcaat caaaaaataa tttctcagcc gggacatatt gttgtaaaca aagaaacagt
     2581 agaggcgtat accactaaag gaatggatta ttttttatat gattattctc ctatcgcttc
     2641 attggagaat aaggagcata taataaaaca cgaagcaaag attggcagtc aatgggtaaa
     2701 ccttccttat aaaacaactg gtagttctat catatataga gtaactaatg agaagagcca
     2761 agtaatcaca cttcctgtat atgcttattt aggaacgaat gtagaaataa atcatcaacc
     2821 tgtaaaggag caggtgaatg ctaatggatt ggtacaaata agcggagtca aaggagttgc
     2881 tgatattaaa gtaagcacaa gttatacagt aacgactatt at
//
LOCUS       DAYYAR010000217         2819 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000217 DAYYAR010000000
VERSION     DAYYAR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.221"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACHAT0_002814"
     CDS             <1..963
                     /locus_tag="ACHAT0_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8452702.1"
                     /translation="PINTFAYTINNEFNLGPNEGSSQVANNQYILLHETANETATGRN
                     EAQYMKRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIYRPDVNQAYNTLGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
     gene            1267..1674
                     /locus_tag="ACHAT0_002815"
     CDS             1267..1674
                     /locus_tag="ACHAT0_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452703.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            1688..2089
                     /locus_tag="ACHAT0_002816"
     CDS             1688..2089
                     /locus_tag="ACHAT0_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452704.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            2091..2462
                     /locus_tag="ACHAT0_002817"
     CDS             2091..2462
                     /locus_tag="ACHAT0_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452705.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(2498..2797)
                     /locus_tag="ACHAT0_002818"
     CDS             complement(2498..2797)
                     /locus_tag="ACHAT0_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8452706.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         1022 a          435 c          509 g          853 t
ORIGIN      
        1 cccattaata cgtttgctta tacgatcaac aatgaattta atttgggccc aaacgaaggt
       61 agctctcaag tagcaaataa tcagtacatt ttactgcatg agacagctaa cgaaacagca
      121 acagggcgca atgaagcgca gtatatgaaa cgttcatgga ctagtgctta cactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc taacagtccc gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tagctagaga ttcagcaatg
      361 aaatatggta ttccacttac actagacaca ccgtataatc aaccagggat caaatcgcat
      421 ttatgggtaa cacaaaatat ttggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcctat gatttggctc atggatttac cgatgaaaat
      541 ccgacaactt cagatgataa accagtcatt gatccaactc gagcaggtgc tgcaaatcct
      601 accctgacag atggaacaaa ttacgcccac attgatcagt tcggagaaat cgaaaacgca
      661 aacttacatg tggctggttg gcacatcgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaaaagctg atggaatata tagaccagat
      781 gtaaatcaag cttataatac tttaggaaat gttggctatc atgtatcttt taacatgcgc
      841 aacttcccta ataagaaagt ctatgtaatg atgcgggcaa cgaatgatcc agaagggaac
      901 actaaaggcg gagcacaaga ttttcatgat aaacgctggt atttaaatat tccgcaacga
      961 taaaaatagc ccctcatttt tgaggggaag tacatatagt tgttgatata gttgctaata
     1021 ttgttagcta atttgttagt aaaactgtat acgaatttag atagtaaaaa aacagtttgt
     1081 tccttatata gaggcacttt tactactgat taaatgtcag atatgcttac aaatatactt
     1141 ataaatatgc ttacaaaatt agctaatatt tccgttgaca aagctttgtt actattgtta
     1201 aactaaattg actagcaaaa gtgaaacata aatttattaa ataaatgtaa aaattaggag
     1261 aaatcaatgt cttatagagt acaattaata atttcagaag atgtagaaag tcagcaattt
     1321 ggcactaatg ttattaataa agtaattaat ccagctcttt ctattaatgc gcctttaatt
     1381 cctactgctt tatcattttc tgtaacagct gtggtgtctg agatagagga tacagaaaag
     1441 ataaaaatag tagaaattga agttttaaac aagaatgaaa aacaaatttt ttctacgggt
     1501 gaagtatcgg taagcttgcc tcctcaagtt aacgatataa actttaatat aaacgccaga
     1561 aatgtcttgg ttgaagaagc aggagagcat tatgctgttg ttaaatttaa tggaactgag
     1621 attggaaggc aaatctttga tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac
     1681 gatacggatg cttgatatag taccatcaaa tacacatcgg agtggtagct taataaattt
     1741 tgctgcgatt tttgcttgcg catcggtaat tctagcatct ccagttgttg tggatgcaag
     1801 ttctactccg aaaactaaaa atgataatca acttgtaatt actacaaaaa atgagattaa
     1861 cacaatttca agtaatgatg gcaaaatttt tgatgtgata aatacggttt taaaaagaaa
     1921 agctgaaatc aatataaaat atgatgaaga tttaaacttg tatttctttg ttatcaaaac
     1981 aacatctgaa ttgttttcct cagattacga tgttttggat actctcgata atgtattatc
     2041 tgactataaa tatatgggga aatctgtagt agcaactttg ggggaataaa atgtttgact
     2101 ttaatgaata tttatctatt tccaaacaaa ttgaatcaga tgataaatat aattctaaag
     2161 aatcttatag aagaactgct gtttcaagag catactacag tgcttttaaa aaatctgatg
     2221 agtatttgaa agaaaattat gatattattt ataatggaag tagcggaaaa ggaagccacc
     2281 aaaccgtttg gaatttgttt tcaacagtta aggaattgaa taccttagga atacataata
     2341 gtgggtatag aatgttagag aaaagaaaat gtgctgacta tattcctaat gaaaccatta
     2401 cgaaaacaga tatggcgctg atgaatcgag aagcagaaaa aataataaac aaactcacct
     2461 aatgaatttc aagcgagttt gtttgttatt tttttattta atctttgtat ctttctggat
     2521 caacgaaagt atactttata tagtcataac gccgatgatc gctacgtgcg tccggcacgt
     2581 cagtcacgat atcaaacaaa aagtatacat ccttcttcat tctagttttc gcagcaggaa
     2641 ttttgaaata gttcttatta gaatagtaga gattgattaa taagctatct tcgattgcta
     2701 aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta ttcgaaggat
     2761 cgaatttaaa ccataattgc gtctcattaa aaagcatagc cattactccg atctatttt
//
LOCUS       DAYYAR010000218         2812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000218 DAYYAR010000000
VERSION     DAYYAR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.222"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHAT0_002819"
     CDS             85..972
                     /locus_tag="ACHAT0_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8452707.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHAT0_002820"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHAT0_002820"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8452708.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHAT0_002821"
     CDS             2274..2720
                     /locus_tag="ACHAT0_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8452709.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          939 a          454 c          609 g          810 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc tt
//
LOCUS       DAYYAR010000219         2801 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000219 DAYYAR010000000
VERSION     DAYYAR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.223"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..1035)
                     /locus_tag="ACHAT0_002822"
     CDS             complement(88..1035)
                     /locus_tag="ACHAT0_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8452710.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
     gene            complement(1040..1792)
                     /locus_tag="ACHAT0_002823"
     CDS             complement(1040..1792)
                     /locus_tag="ACHAT0_002823"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8452711.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            complement(1793..2701)
                     /locus_tag="ACHAT0_002824"
     CDS             complement(1793..2701)
                     /locus_tag="ACHAT0_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFM8452712.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
BASE COUNT          913 a          526 c          349 g         1013 t
ORIGIN      
        1 ttctatccca gatttgattt tacaaaactc atcagtcctt attgacaaag agcctgaatt
       61 taccctctaa tcttatctta gtatgtttta attgatttca gttttcaaat aattagtcat
      121 tccacttaaa ataccatatt tcggttgata gccaattgac tggatcttag taatatctgc
      181 tagagagtca tggatatcac ccgaacgctc ttctttgtat tcaagtgtca attctacacc
      241 gagaatttca ttgattgcat gaatcaattc caaaagtgtg gttgctttac ccgttccaac
      301 attgaattgt ttccccagtg cagcttcctc attcgcaact aaaagtaatg cttggattac
      361 atcatctata taaacaaagt ctctagactg gcttccatca ccaaataatg taaatgaagt
      421 tgcttctcct acgatttgtt tcttatagcg atctacgaga atagaaatca caccactgta
      481 tggagaattc ggattttgat ttggaccata gacattgaaa aaacgaactg cgctaccggg
      541 tacatcatat aaatgacaat aatctagcac atattgttct gcagcgaatt tatcaaccgc
      601 ataaggcgtc aatgggcgaa tcacggactc ttcttttttt ggtaaagtcg gttcatcccc
      661 ataaacagcc gctgaagaac taaacacgat ccgttttaaa tcaggttgat acttgcggat
      721 caattctaaa atcattaata cactttcaaa attcacacga tgtgtttcta ctggtcgggc
      781 aactgaatct gccacactag caactgctgc taaatgaaag atataatcaa atttattagc
      841 tttcatgatc tcttccatca aggatttctc agcgacatct ccctcaataa aagtgatatt
      901 tttcgtcata tcaagatttt ctacttttcc cattgataag tcatcgacga cgattaccac
      961 atgttcttct cctaaatgat tggctaaggt agaaccaatg aagccagccc caccggtgat
     1021 aagataacag ctcatccttt tacctccgat ataaataatt atagatatct tgaagctccg
     1081 aagaatcttc taaaaattta ggaaacaaaa attctaacca tttaaaatct tcttcactat
     1141 caatatctaa aattagataa tctctcattt ttattatccc acattgccca tcaaaaatat
     1201 aattactttt ttttagaaat tcaggtttaa aagaatacat tgaggcattc atttcaaaaa
     1261 cttcaggtgt ctgttgccta gatgtgtatc ttgatttatt aactatagta acttcatttt
     1321 caattctttt taccatatta aaatagggat tacgtcttgc agaaacaact gaaaagtaga
     1381 cgtctttatc taaatttttt agatgttcat ctattaattc ttcaagatta gaaacttcac
     1441 gtaaaggtga agtcaaatct aaatctataa ttaaatcaaa tatattatct tctctttcta
     1501 gttgtaagaa tgaatcacgt atcacttcaa ttttccctac agtatctgta gctaattcag
     1561 gtttacgttc tacaattttc acagacggat ttaacttagt cgtcaattct actaactcat
     1621 tactgtccgt attcaaacaa gtcgtgcaat tatgttcagg atgtttttgt tgatagatat
     1681 ctatcattgc taaagcgaaa tggactaggg gctttcctgc caaatatttc aaatttttat
     1741 ttttaaaccc cttagaccca gctctaccac aaatagtaaa taaaatttcc atttatttct
     1801 cctcagctat tttcaatact tcgtttgcat gatataaatc attccagttt tctttgcctg
     1861 ttactacgtt ttcaaagaaa tatttcaact ccttctcata catatcatta gcagattcag
     1921 gcatttcttt aacaatgttt tcttcaaaac ttattttatt atttaataag tcaacattga
     1981 tacattcatc taaagtaaac aattgaaatt ttcttgtagg aactcttcca aaataatcta
     2041 aatgaatttc tataatttta tcatcgtatc tcaatatata tgttgctaaa tcattactgt
     2101 taatttcaag attcgaatat ttaccaaact gtttttttac tgatttaggg aaaccaaata
     2161 ggtttataat ataatccaat tcatgtatac agtctaattc tactcctccc cccatagatt
     2221 taaaggcaga ataattattt ctataatcag taccttttct ccaatcagga agataagtag
     2281 aacaaatagc tcttacagaa tatacttttt tactttgaat actattacga acaacttcca
     2341 tgatattttt atacctcagt ggagcagcaa cataaatatt tttgtaatca taattatatt
     2401 gagtaagatc aatactctca gaaaaagcag gtttttctaa aaatatgtaa tcaacttttt
     2461 caacaattac atttagtgtt tccagatgct taaaagtcgg atttgtaata aaagctatat
     2521 cataatgtgt atccaaatcg tctctatttt gaatatacgt tatttcttca aactcttttt
     2581 ttccactgct actataagct gttatatcaa aattaataga attctttttt ccatagctta
     2641 ttagattttt tagatgtctc tttccaattg accctaaccc aaaaaaaatt actttcatca
     2701 ttaatttacc tcaatgtcgt taattaagtt aataatatac ttatcttttt caaaagtata
     2761 ttggggttcc attttctttt ccaaaagcgt gaaaaattct t
//
LOCUS       DAYYAR010000220         2796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000220 DAYYAR010000000
VERSION     DAYYAR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.224"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACHAT0_002825"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACHAT0_002825"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8452713.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1085 a          435 c          569 g          707 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattgg
//
LOCUS       DAYYAR010000221         2574 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000221 DAYYAR010000000
VERSION     DAYYAR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.225"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1022)
                     /locus_tag="ACHAT0_002826"
     CDS             complement(45..1022)
                     /locus_tag="ACHAT0_002826"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8452714.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="ACHAT0_002827"
     CDS             complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="ACHAT0_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8452715.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            2296..2553
                     /locus_tag="ACHAT0_002828"
     CDS             2296..2553
                     /locus_tag="ACHAT0_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452716.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
BASE COUNT          701 a          537 c          475 g          861 t
ORIGIN      
        1 aatttaaatt gataagccat gactttgttt cgtcccttct tttcttaaaa ttccgcgatt
       61 tcttttactt tgtcttcgat atcttttgca tttggcaacc agacattttc tgcttgtcca
      121 aatgggaaga ctgtatcagg tgcagaaact cgtccgattg gggcttctaa tgaaaggata
      181 gcgcgttctg agatctcaga aactacttgc gcggcaacgc ccgcttgtcg ttgtgcttct
      241 tggacaacaa ctacgcgacc agttttttcc acggattgaa tgatcgtttc tacatctaat
      301 ggcgcaactg tacgaagatc aatgatttca acagatatat tttcttttgc aagattgtct
      361 gctgctttga ttgcttcacg caccattgct ccgtatgtga taatagatac atctgttccc
      421 tcacgagtaa ctgctgcttt atccaaagga acttcgtaag cttcatccgg cacttcttct
      481 cggaatgagc gatatagttt catgtgttct aggaaaacaa ccggatcgtt acttctaata
      541 gaagaaatca atagtccttt tgcatcataa gggttggaag gaatcactac tcgaatacca
      601 ggagactgag cgatcaaacc ttctaaatta tccgaatgca attctggtgt atgaacaccg
      661 ccaccaaatg gcgaacgaat tgtgatgggt agatttctag tgccgctcat tcgatatctt
      721 gtacgagcca tttgagctac tacttcgtcc attgcttcga aaataaaacc aaagaattgg
      781 atttcaggaa ctggacgaaa tccttctaac gcaagaccga aactcaatcc tgcgattcca
      841 gattcagcta aaggagtatc gaatactcgg tcttctccga acttttcctg caatccttca
      901 gttgcccgga aaactcctcc gtttttccca acatcttcac caaaaacaac gacattctcg
      961 tcattttcta gttcaagtgc taaggcatct gtgattgctt ggatcattgt tttttgtgcc
     1021 atgattattt cgactccttc gcttcataaa ttgcaatttg ttctttgatt gattgtggag
     1081 atacttcgaa catgtttttc aagaaatctg aaaccttttg ttttggtact ttgtcggctt
     1141 cagcaatagc tgctttgatt tcttctttag ttgcttcgat gacttgttct tctttttctt
     1201 cagaccaaag tcctttttcc gtcaaatatt tacggaagcg gatcaatgga tcttttttct
     1261 gccaatcgtc atctgtgtct tttgtacggt agcgggttgg atcgtcacca gatagtgtat
     1321 gtggtccata acgataagtc agtgtttcga ttaggactgg tccatttcca ctgatcgccc
     1381 attcccgtgc cattttagat acagtatata ctgcaagcgg atccattcca tcgacttgaa
     1441 taccaggaat cccagctgcg acagcttttt gtgcaagtgt ttttgctgca gtttgttttt
     1501 cacgaggtgt agagatcgca tacccgttgt tttggatata aaatacggca tttgcatggt
     1561 aagcgccagc gaaattgatt gcttcgtaaa aatcaccttg tgatgaaccg ccatcaccag
     1621 tataagtgaa gacgacattt ttcttgtttc gtttcttcaa acctaatgca acaccggctg
     1681 cttgtacata ttgtgcaccg atgataattt gtggtggtag agctttcaat tcttccggat
     1741 aagaattacc tgcagcatgt cctcgagacc ataaaaaagc ttgagataaa ggtaaaccat
     1801 gttttactag ttgaggtaca tcccgataac ctggaagaag aacatcttct ttatcaaatg
     1861 cgtaagcact agccagctga cttgcctctt gtccagcagt tggtgcaaaa aagcctaggc
     1921 gtccttggcg gttcagcgct gttgaacgtt ggtctaatac acgagaccat accatatgag
     1981 tcattaattc aacgagttca tcatcggata aatcaggtac taaatctttg ttaactacgt
     2041 ttccatcttg atcaagtgct tgatatactg ggaaatctgc attgacttca tctagcagtg
     2101 cttggaagtc gatcggtgct tttttgtttg ccatttgtca cacattcctc tctcatctat
     2161 aagaaaatct cttcattctg cgattgaaac agtgaataaa actgtatcac tatttaaccg
     2221 tcttcattaa taaattatca tggatttatg agaaatacaa gctaaaagca caaattttca
     2281 cgatgaaagg aaattatgcc aatccgcata ttaacagcat tagtgaataa aatcacaata
     2341 aggaaagcgt tttatatttc atctgcattt tttctatttt ctaatcaaac ttacaaccat
     2401 ctgtttctat ttaaaaacgt tggtgttata cagttttgtt gcaaacattt attttattta
     2461 cacatcgtga acggacgtac aaataatggt tttttcttag tttctttata tcgtattctt
     2521 tttccgaaaa aaaagaaaca gcttgaaatt taaatccaag ctgttatatt cttt
//
LOCUS       DAYYAR010000222         2503 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000222 DAYYAR010000000
VERSION     DAYYAR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.226"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..411)
                     /locus_tag="ACHAT0_002829"
     CDS             complement(46..411)
                     /locus_tag="ACHAT0_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452717.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            619..1101
                     /locus_tag="ACHAT0_002830"
     CDS             619..1101
                     /locus_tag="ACHAT0_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452718.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1298..2188
                     /locus_tag="ACHAT0_002831"
     CDS             1298..2188
                     /locus_tag="ACHAT0_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8452719.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          845 a          417 c          501 g          740 t
ORIGIN      
        1 taaaaagaag aaagtagctt ctctttgtac atatatggtt tgtttttatg gatgccgagc
       61 ttttttataa gataaggcta ccgcccataa cgacccaagg atttgtggaa ctaagtaaat
      121 acctgcaaga agcaagatca gctgggtcgg ttttaaagga tgatagaagt aacgccattc
      181 agtaaaattg gcgcaaaata aataggaagt gacagtagat ataatattgg caatgaataa
      241 agatataata cgcctgttcc tttgaaaata atatccagca aaaagagata aaaatatcaa
      301 tgtgatgaac ccgcttacgg aatgataacg aaaatcaagc agtaaggcga aatgcacata
      361 aggaacaaaa gataaaagca acggaaggtt tttccagcaa gtttttatca tctttcatca
      421 ccacctaaca agtgttatta ttttgttagt tttattgtaa attgaatcct gcaataatca
      481 attacttttt cattatttct agattaaatg aataaactct catgccatta tctctaaaaa
      541 aaaagttttt ccccctaatt tttttagata gaaaattgta tagtatgatg agagaaaaag
      601 tagtttgggg gaacgttaat gagaagaaaa gcagtaatcg atcggcttta tttatttttt
      661 tggttttttc ctatcgtttt gctacctttt acatgggtaa atgctgttgt acaaggtgcg
      721 gagatattag gcctgatcca aggaagcgga tttgtggttt ctaggaattt tccagctttg
      781 attgttttct atttagtgat aagtgtaggt ctcttattac caaaatctct gatcaatcac
      841 tacaattggc agctgcttta cttttcttgc ttattagtat ttacatctac gttttctttg
      901 ctgcctcgct tgattatcgg ccctaatggt ttgagcgaga gtggattgaa ggatttagct
      961 tattcctcca tgtatttctt gacgatcaaa ccatggttct atttagtaat tgcctcattt
     1021 attcttgcaa cattttttta cttctggaca gaaatcagga taaaaagtga acgatctgta
     1081 tctataaaaa ataactattg atggtggatc atcagtaaaa aaacatatga gctgcgtaaa
     1141 atgcatgttt ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa acgtttacat
     1201 gatttttaca taacttaact atatttttac agcaaccata cgagggattt acagtcgact
     1261 gataaacttt agttgtaaat aagaaaggag caataaaatg aaaaaagtat tgtttactct
     1321 aggtatgatg ggaattcttt tagccggatg cggctcagga tcaggaaata cagatggctc
     1381 tgccacacaa aataattcaa gtgactcaaa tgaacaagta aaaatcgttg cggtaggttc
     1441 gacagcatta cagccgttag tcgatgcagc acaagaaagc tttgtacaag aaaatccaaa
     1501 ttatcaaatc tctgtacaag gaggaggaag cggaactggt ttgagccaag tcgaagctgg
     1561 agcagttact atcggaaact ctgatgtatt tgcagaagaa agagacggag ttgatgcttc
     1621 aaaattagtg gatcataaag tagcagtggt cgggatggca ccaatcgtca ataaagatac
     1681 agatgtcaaa gatatcacca aacaggaatt gattgatatc tttacaggaa aaatcactaa
     1741 ctggaaggaa gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg caaacggcag
     1801 cggtacccgc gcgacattcg aaaaatgggg actggatggt gctacccctg tgcagtccca
     1861 agaacaagat tcatcaggaa cagttcggca gctggtaagt caaacaccag gagcaatcag
     1921 ctatttagct ttctcatatc tcgatgattc cacacaagca ctaagtatcg acggtgtaga
     1981 accaaaagaa gagaatgtag cagataacag ctggggaatc tggtcatatg aacatatgta
     2041 cacgaacgga aaaccttccc ctgaagtcca aaaattctta gactacatga tgacagaaga
     2101 aatccaagaa ggacctgtca aagaattggg ctatttacca atcacaatga tggaagtaga
     2161 acgtgatcac gaaggaaata tcaaataact agcagataaa catgccacgg tagtcgttac
     2221 tattaaggaa aaatcaacta tgtttcccca catatacatt tttcctttaa ttgtgaaaaa
     2281 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg
     2341 tttattcgaa aaaaagagct acgacataga actgtcgtaa ctttttttgg ctcttcgtca
     2401 aatcgtgttg gtgaagacaa atttgaataa aacttagacc gatatgcggc gcgttctgtg
     2461 cgccgcatat ttttttagtt ttcaaagatc agaccttgtt gta
//
LOCUS       DAYYAR010000223         2408 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000223 DAYYAR010000000
VERSION     DAYYAR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.228"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..2183
                     /locus_tag="ACHAT0_002832"
     CDS             30..2183
                     /locus_tag="ACHAT0_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8452720.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          825 a          428 c          538 g          617 t
ORIGIN      
        1 atgaatgtaa ataggcggaa ggataggaaa tgatgaaaga ttttatgaaa aaaatattaa
       61 atacttcttg gttagaaaaa ataagaaaag gtgcacaaga acctaagaac aaaacgtttc
      121 gccggtccca tgttcctttt cgcttaaatt ttcttttttt cattatattt acgttatttg
      181 ttgcgttgat tgctagattg ggttatttgc agatcgtgaa cggcgaagag atggaggcgc
      241 gagtgaagtc tacttctacg attacggtcc aagaaagtac tcctcgcgga atgatctacg
      301 actccacagg aaaagcttta gttacgaata aagcgaatca ggccatcaca tatacgagag
      361 gaacacaagt tacggctaaa gatttgcttg aaatcgcaac gaagctgaac tcgctgatcg
      421 atgttccggt agatgaaaat ctgacagata gagataaaaa agatttttgg ctggctgatc
      481 ccaaacattt gaaagaagcc acaaaaagac tttcggcaaa agaaaagcaa ttagggaaca
      541 gcgaacaata tagtgcgatg gtcaataaag tcacagctga tgagattaat ttaaacgagg
      601 agcaattaaa aattgctacc atctttaaac ggatgaatag tgcatatgaa ttaaacactg
      661 tctatatcaa aaactctggt gttactgatc aagagttagc tgttgtggca gaacgcgctt
      721 ctgaacttcc aggagtttcg actggcacag actggacacg agaatataat gcagcaagtt
      781 cactgaaaag tattcttggc agtgtgacga ctgaaaagca aggacttcca gcagatgaag
      841 cagagaaata cctagcaaaa ggttattctc ggaatgaccg tgtaggacaa agttatttgg
      901 aaaaacaata tgaagatgtc ctgcaaggaa ccaaaaccca atatgaagtt tcccttgata
      961 atgaaggaaa tgtttcgaat caaaaagaaa tcttctctgg ggaaaaaggt tctaatttaa
     1021 tgttatctat gaatgcagaa ttccaatcaa aagtagaaga aattttgaaa aggaattatc
     1081 aaacattgat caacaacggc aaagcacagt attcgcctgg tgcttatgca gtggctatga
     1141 atcctcagac aggagaagtg ctagcaatga ctggtttcag tcatgaacaa ggttccaaag
     1201 aaatcaccga gaatgcttta ggcacgatta ctagcgcctt tgatccaggg tctgtagtca
     1261 aggctggtac tttgacagca ggctgggcaa gtaacgcgat cagcggaaat caagttttga
     1321 ttgatgagcc gattcgtctg caaggtgctt cagaaaaaag ctctgtcttt aatcgaagcg
     1381 gacaagttgc tcttgatgca gtcaaagcat cggaattgtc ttctaatacg tatatgatca
     1441 aagtggcgtt aaagatgtta ggactagact atacacctgg aatgggacta cctagtttag
     1501 atgaagaagc taaagcttat cagcaattga gagactcttt caaagagttt ggtcttggta
     1561 caacgactgg gatcgatttg ccgaatgaat ctcctgggat ctcacgttct gttgattata
     1621 tgaagaagtt caatgcggat aatggcaaag aatggtatgc tccaggtaac tttaccgatt
     1681 tggcatttgg acagtttgac acatatacac caatccaatt agctcaatat gcttcaacag
     1741 tcgctaatgg cgggaaacga gtacagccgc acctagtcaa agcgatctat ggcaatgacg
     1801 aaaatggaaa cttgggtgaa gtgaagaaag aaattggcac aacagtagaa aatacggtca
     1861 atatttctgc agaaaatatg gcgatcctcc gtgaaggatt ccaccaagtt gtccatggta
     1921 ccgatcctta cacaacagcc aaaccattag ccagtgcgaa aatggacctt tccgcaaaaa
     1981 cagggactgc agaaacggtt gcggaaggtc atccagatat tgcgacagtc aatagtaata
     2041 tcgtggcata cggaccaaca gacaatccgg aaattgccat cagtgttgtc ttgccgaact
     2101 tattagacga acaagaccac ataaacttga cgattgctaa agaaataatg gacgcgtatt
     2161 atgatatgtt catggcaaaa taaaaaagag gtcgtgaaaa agccgcttag tttcgagtat
     2221 gaagaaaaat tttgtgaaaa tagcttctca catttttgct taaatttgac ttcataccga
     2281 gaaactggct tttgaacacc gtttactcgg caaaaaaggt tgtgacaaaa attgtggctc
     2341 tttgtcaata aggactgatg agttgtgcaa aatcaaatct gagtcagaat gaaccacatt
     2401 ctggctca
//
LOCUS       DAYYAR010000224         2400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000224 DAYYAR010000000
VERSION     DAYYAR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.229"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHAT0_002833"
     CDS             207..2069
                     /locus_tag="ACHAT0_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8452721.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACHAT0_002834"
     CDS             2069..>2400
                     /locus_tag="ACHAT0_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8452722.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAYYAR010000225         2391 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000225 DAYYAR010000000
VERSION     DAYYAR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.230"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            225..1838
                     /locus_tag="ACHAT0_002835"
     CDS             225..1838
                     /locus_tag="ACHAT0_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8452723.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          751 a          425 c          454 g          761 t
ORIGIN      
        1 tttcattcga tccatcattt ttcttcagct tttaaaaaat atacagggtt atccccttca
       61 aagtattatc tatccttaaa atagaaaaaa atttttaggt tttatccaca aattatttct
      121 ctatattttc tgtctattca aaataaacaa taattttccg gcacaattat ttgctgtttt
      181 ttaccataac atagtacgat caacgtagaa aaggagatgt ttttatgaaa gttgttgtca
      241 ttggcggtgt agctggcggt ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc
      301 acgaaatcat tatctatgaa cgcggagaaa atatttctta tgcaagttgt gctttgcctt
      361 attatttagg aggtgtgatc actgaccgtg actcgctgat cgaacgtaca ccagaaatat
      421 tgaaaacaaa aaacaacatc gatgtcttta ctaaacacga agtaacagca atcgatcctt
      481 ctactaagcg attaacagtt aaagacctat ccacaaatga agaaataaaa acagattacg
      541 ataagttgat catctcttct ggtgctagac cagattatcc ggatattccc ggagtttttg
      601 aagcagaaaa cggctttgtc ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc
      661 ttgaagaaaa aaatccacaa catgtcgtca ttcttggtgc aggtgttatg ggtctggaat
      721 tagctgagaa tttcaagcat cgcggcttag acgtgacttt aatcgatcaa ttgccacaag
      781 tcgctttccc ttatgatcca gaaattgcta atttagttta tgacaaattg ctgaaagaag
      841 gattagccgt tcatttagaa acaagagtta ctgagatccg tgataaaggt cgagaaatag
      901 tattatcaga tggctccgtc ctttctgctg atatgctaat ttttgctgtt ggtgtttctc
      961 cgaagaatga agtggtgaaa gcaaccggca tacaattatc agatacaggt cagatcattg
     1021 ttgatgacca gttacaaacc aatctgccgg acatctatgc gattggcgat attatcgaaa
     1081 cgactagtgt agtgactggt cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc
     1141 aaggacatat gctggcagat attttaaatg gtacgcctat gcgctatcgc ggatatattg
     1201 gtgcaggtgt cgcaaaaatc tttgatcata cagcaagtta tgcgggaatg acagaacatg
     1261 aattaaaagc atcaggcata acaaattata aaactgtttt tatcactccc tttgaccatg
     1321 cctatttcta tccagaagct acaagattaa atctaaagct gatttttgat gcagatagcg
     1381 gtcgtatttt aggtggacaa gcatttggag aaaaaggtgt cgataaacgg atgggagaac
     1441 tttctgtagc aatcaccgga aacttgacag tctttgattt gcctgatttg gagttgcctt
     1501 actctccacc gtattctact acccgtgacc cgttgaatat agctggttat gtcgcaatca
     1561 atcaaatgac gaatatcgta gaaacgatca aagcaagtga tatacccgaa aacgatttga
     1621 aagaagcgtt ctttttagat atacgtgaac ctaataaagc accttctggc agtatctcgg
     1681 caacaaaaaa tattccaatg aatgagttac gagatcgaat caatgaaatc ccaaaagata
     1741 aaaaagttta cattactttc agaagaggat tgaatactta tacttctgcc cgaatcttgg
     1801 caggtttggg tatcaaagcg attttgattg aagaataaaa aaacgaccgc ggtgggtagc
     1861 cgcggtctag gagttatttt tacttgagga gtaaaaatga aaaagtatat agggttgttg
     1921 gttggtatgt atatagaata gaagataaat gtgaagaaat tgtgcattat ttcttcacat
     1981 ttattaggtt atagtttaag aaagtttaaa cattgcgcaa tttaattatt ttagctgttt
     2041 cttttcataa aaaaacatgt tgtgactctg agacaaatca tagtccatag tgatgtctct
     2101 agagtaacta cgcctaatat tacgaggcat catattaatc tatcagcact agtacctaaa
     2161 ctagcatccc tttcgaatat tttttcttat ataataaagt ttgctttcac tcctctaatc
     2221 tcaccaaatc gactggcttg cgttgtgtga tactaggaac tgattggttt agaacgactt
     2281 ttctattgta atcatatttt tcgccatcct cgttgtcttc taatccttca atgaatgatc
     2341 cctaatgtgt gtattaaata atttaatttt tttgttccta tttatttaac a
//
LOCUS       DAYYAR010000226         2365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000226 DAYYAR010000000
VERSION     DAYYAR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.231"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1451
                     /locus_tag="ACHAT0_002836"
     CDS             150..1451
                     /locus_tag="ACHAT0_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8452724.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1555..1755)
                     /locus_tag="ACHAT0_002837"
     CDS             complement(1555..1755)
                     /locus_tag="ACHAT0_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8452725.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            2151..2300
                     /locus_tag="ACHAT0_002838"
     CDS             2151..2300
                     /locus_tag="ACHAT0_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452726.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          768 a          454 c          408 g          735 t
ORIGIN      
        1 cgtacaaaaa aaccgtcttt tcttggctct ataatttcta ggactgatag aaaatcattc
       61 tatcggtccc ttttgagcat gaaaaaacaa aacatacaga agctccgtta gaattgaatc
      121 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcatatc aaactactac
      181 ttggaatcaa agataaaaac attttcatca caaatgtgga atcgaagagc attaagaata
      241 tcaagagtct tgttgtttct gcgacgttaa gtaaagaaat tcgtcgctgt cctttgtgta
      301 aacagatgaa tcatgaaggc atgatcgtta agaacggaaa gaaaaaatcg ctcattcaac
      361 ttaataaatg tgcgaaccaa ctgacttatc ttgctttagc gaaacaacgc taccactgtc
      421 gtggctgcca cacctatttt acggcgaata cctacattgt cgatagaaac tgctttatcg
      481 ctaaacaagt gcgctataaa attttagaag agctaactga aaaacaagca atgacgacaa
      541 ttgctaagca ttgcggcgtt tcttggtcga ccgtttctcg gacactcgct tctctgatcc
      601 ccatgacaaa agtaaaaaga aattggttac cgcgttgttt attaatggat gaatttcgtt
      661 cactgaaaaa tcaagtcggt ccctactcct tttcttgtat ggatggcgat actggaaaat
      721 tactggatat tcttccttca cggaagaaaa aggctctcgt ttcttatttt atgcaatttg
      781 aacgacgcgc acgattgaag gtaaaggtca tcgtgacgga catgaatgtt tcttatgcct
      841 ctctgataaa ggaatgtttt ccaaaggcaa aactcgttgt ggatcgtttt cacattgtca
      901 aacatctcat tcgtaaattt gaggatattc gcgtacgaat catgaaaagc tttgatcgaa
      961 acgatccaac acaagccaaa tattatcgac aattaaaggc gttgtctcgg cttctgatca
     1021 aacgacagga caagcttgtt tatgacaaat ggaccaaatg gcgcaatttt ggttgggcgt
     1081 atctcaccga aagtgaagtg gtggatcgtc tgctttcgat ctctgacgaa ttacgaatcg
     1141 cctattttta ctatcaagaa attcttcaag cgtttcacga caaagaggcg gacactttct
     1201 ttaaacttgt aagaacgatg cccaattccg ttccaaaaga actgcattct ataaaaaaag
     1261 cttttataaa ctatgaatcc ggcattcgtt tagccctcga attgccttat tcaaatgcca
     1321 aaatagaaaa tctgcatacc cacatcaaag cattgaaacg agtcgcttat ggctttcgaa
     1381 gctttcggaa aatgaagacc agaattttct tactaaataa tctgattacc tatgaatcaa
     1441 aaaacatcta aagtagaaaa ggaattctac cttagatgtt cttatctcac tctatcagtc
     1501 ctatttgaca aagagccctt ttctttcgaa aagacggtta aaagtcaatt tttattaagc
     1561 tttaacaatg tttgttgctt gagggccacg ttgaccatct tcaacatcgt aagacactgc
     1621 ttggccttct tctaaagttt tgaagccgtc accttggata gctgaaaaat gtgcaaatac
     1681 atcatttcca tcttcaccag tgataaagcc aaaacctttg tctgcgttga accattttac
     1741 tgtaccgtta ttcataaata aattcctcct gatacgtatc aatgtacgtt ttttcgttgc
     1801 aataaatatt tgataggcaa aaggagtttt atctatgtag atatatcgct caaattacgt
     1861 ttcaaaatag attactcatt taatatatca tgctttttct aataacacaa ggataatgca
     1921 atgaaatata aaattcattg tcttctattg tcttcgaatc gaagaaaaag ttgaataacc
     1981 gcgattgatt cactttttgt tttcttgttt atttgctttt atctgcaact tttgacttta
     2041 aaatttctta actatgaaaa cctttcaatt ggtggtatac ttataaatgg aatttcgtta
     2101 tcaacgcgtt cgttgccttt cagcaagtat ttaaagaaag gagaatggct atgacattct
     2161 ataacaaaga tatacaatat cgagtaacct tggatacaga tttgaatcta tttgtcgttt
     2221 ttgacaaaga aaataccaat catgtagcga caggtgttac gatcgaacaa gcagtgcaag
     2281 aattaaaaaa atctgcataa ggagtggaaa ccctcatatg gacgattctc gttcttctct
     2341 ttaaacgcat ctactggtag ataac
//
LOCUS       DAYYAR010000227         2340 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000227 DAYYAR010000000
VERSION     DAYYAR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.232"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1657)
                     /locus_tag="ACHAT0_002839"
     CDS             complement(365..1657)
                     /locus_tag="ACHAT0_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8452727.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="ACHAT0_002840"
     CDS             complement(1931..2191)
                     /locus_tag="ACHAT0_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452728.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          781 a          441 c          355 g          763 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
//
LOCUS       DAYYAR010000228         2303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000228 DAYYAR010000000
VERSION     DAYYAR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.233"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACHAT0_002841"
     CDS             191..445
                     /locus_tag="ACHAT0_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8452729.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACHAT0_002842"
     CDS             446..763
                     /locus_tag="ACHAT0_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8452730.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="ACHAT0_002843"
     CDS             1322..2257
                     /locus_tag="ACHAT0_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452731.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          840 a          311 c          433 g          719 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag aaaaatgtct
     2281 aaacatgttg gtaaatcaac aaa
//
LOCUS       DAYYAR010000229         2263 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000229 DAYYAR010000000
VERSION     DAYYAR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.234"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..885
                     /locus_tag="ACHAT0_002844"
     CDS             <1..885
                     /locus_tag="ACHAT0_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8452732.1"
                     /translation="FKLFFNYCNILKKIKPDMVIAYTIKPNIYGGLACRLRKVSYALN
                     ITGLGTAFQKKGVLKSLIVFLYRLAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLN
                     GAGVDVEHFSYLEYPKDEQVHFLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGY
                     FEEDYGIKIEKYEKEGWLKYHGYQSDIRPFVERAHCFVLPSWHEGMANTNLESAASGR
                     PIITSDIPGCKEAVIESKSGYLCERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMI
                     KKFNKKKVVDETIKSLFS"
     gene            892..1287
                     /locus_tag="ACHAT0_002845"
     CDS             892..1287
                     /locus_tag="ACHAT0_002845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8452733.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            1284..>2263
                     /locus_tag="ACHAT0_002846"
     CDS             1284..>2263
                     /locus_tag="ACHAT0_002846"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8452734.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDIIQNLSDEYYTRAICKCKYKDNWKGISAKFALKNKWVFLIYI"
BASE COUNT          894 a          245 c          430 g          694 t
ORIGIN      
        1 tttaaactat tttttaacta ttgtaatatt ttgaaaaaaa taaagccaga tatggtcatt
       61 gcttatacta tcaaaccaaa tatatacggt ggattggcat gtcggttaag aaaggtttct
      121 tatgcattaa atataaccgg attaggaacg gcatttcaaa aaaagggggt tttgaaaagt
      181 cttattgtat ttttgtatag attagcagct aaaaaatcta aggtcatatt tttcgaaaat
      241 tttgaaaata tgaaaacatt tcagacttta caaattgcta aacaaagtaa atacaagcta
      301 ttaaacggtg caggagtaga tgtagaacac tttagttatt tagaatatcc taaagatgag
      361 caagttcatt ttttatttat tggaagaatt atgaaagaga aaggtgtaga tgaacttttt
      421 gaagcaatga aaaacttata tagtgaagag aaaaattgtg tacttgatgt ccttgggtat
      481 tttgaagaag attacggcat aaaaattgaa aaatacgaaa aagagggatg gttaaaatat
      541 catggatatc aatctgatat tcgtcccttt gtagaaagag cacattgctt tgttttgcct
      601 agttggcacg aaggaatggc aaatactaac cttgaatcag cagcatcagg gagaccaatt
      661 attacaagtg atatcccagg gtgtaaagaa gcagttattg aatcgaagtc tggttattta
      721 tgtgaaagaa aaaatagttg tagtctctat aattcgatga ataaatttct aaaattatca
      781 tatgagcagc gcattaacat gggaaaaatg gggagaaatt atatgattaa aaaatttaat
      841 aagaagaaag ttgtcgatga aacaattaaa tcattattta gttaaaaaga tatgttttgc
      901 aaagtatgtg atagaaaaga gggattctgt ggcttcagaa tagatatcaa agaattggca
      961 aaagtttctg atattttttt atttactaca ttacaagaag gaatgccaag atctatgatg
     1021 gaagctatgg caagtggatt gccatgtata gcaagtaaaa tccgaggtaa tgtagacctt
     1081 ttagtggaag gaaaaggtgg atatcttgtt ccagctaaag atgcagaatt agtagcagag
     1141 tgtttgagag aactaatcaa taataaatct cttagaaagt atatgggaaa tgagaacctt
     1201 tccagaatta aagaattcga ggttagtatc gtaaaaaaaa gaatcaaaga aatatatgaa
     1261 gaagttatag gtgagagtgt aaaatgaaaa atccaatgtt ttcaataata gtacctatct
     1321 attgcgttga aaaatatttg aaagattgct tagattcaat aagaaatcag acatttaaaa
     1381 attttgaagc aatactagtt gatgacggta gtcctgacga atgcccagaa atatgtgact
     1441 attattccca aatagatgga cgtttaaaag taattcataa gaaaaacggt ggaataatag
     1501 atgcacggaa aacaggagtc actagtgcta tgggagagta tatagttttt ctagatggtg
     1561 atgattggct tgcaaatact tatttagaaa aatatgcgga agtaattact aagtacaatc
     1621 cagatataat atgtagtggg tattttgcat cacgttccaa caaaattaga aaaagagaat
     1681 tgtcttgctc gtttggatat tatgacaaag ataaaattat aaatttgatt tatcctaaat
     1741 taattgagga tgaacatgga gagtatttta gaccacaatt atgggcaaaa gcttttagaa
     1801 ctactctgta caaggaacaa caacaagtat ctgggcaagt tagtgttggt gaagatcatg
     1861 catgtacaaa accatgtatt taccatgcaa attcgatata tatgatggaa aaatgtttat
     1921 attattacag aataaacgaa aattctataa caaaaaaacc acaaccatat tattgggaag
     1981 gaccaaaggc tattggagag cattttgaaa agcagattga tatgaataag tatgattttc
     2041 aagatcaagt atatagaaat gttacacaca atttatttaa tgttgctgtt tcacaattta
     2101 ataaacagga caaatattta aatataaaaa aagatattat acaaaatcta agtgacgaat
     2161 actatactag agctatttgt aaatgcaaat ataaagataa ttggaaagga attagtgcta
     2221 agtttgcatt gaaaaacaaa tgggtatttt taatttatat tta
//
LOCUS       DAYYAR010000230         2209 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000230 DAYYAR010000000
VERSION     DAYYAR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.235"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            585..731
                     /locus_tag="ACHAT0_002847"
                     /pseudo
     CDS             585..731
                     /locus_tag="ACHAT0_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(819..1112)
                     /locus_tag="ACHAT0_002848"
     CDS             complement(819..1112)
                     /locus_tag="ACHAT0_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8452735.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1149..1334
                     /locus_tag="ACHAT0_002849"
     CDS             1149..1334
                     /locus_tag="ACHAT0_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452736.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1515..>2209)
                     /locus_tag="ACHAT0_002850"
     CDS             complement(1515..>2209)
                     /locus_tag="ACHAT0_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8452737.1"
                     /translation="RMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPM
                     SVLFHVLFYTGCRVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSA
                     RRVLVTPLVMEKLQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHV
                     KRCVNRSGINKPFHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEK
                     VKPLIAFSSLES"
BASE COUNT          688 a          383 c          330 g          808 t
ORIGIN      
        1 aaataatcct ttacacgtta ttctaaattt aatttttatt attttattga tgtatagaaa
       61 gtttggggct ttttttctct ttttattcta aggttatttt agtcttgtcc tttaatacaa
      121 agattttatt tttaaataaa aaatactctt tttttacgat tattgaaaaa aaaagataaa
      181 acttgattaa ttgaagtatt gcttaaattt ggaagctatg agggaagaat aaaagagtta
      241 ggacgggatt atctactatt tttctttgtt ttaaatattt gttatctttg aacataaaag
      301 agaaatgtgt cgactgcagt gttgtgtccc tcatcacgaa taaacggtgt tcaattagct
      361 gatcttgtat tcacttaaaa aatggtaaag tacgagttgc tattccccat tttcaatttt
      421 ctaacgctca ggacaaaata ctgttttcac aacctcattg gaataaaagc atatatattg
      481 tttcaagaac gaaatcttgt tatcttttta aagggcatct gtccaaaatc taattttagg
      541 aggtacgttt tggtatttat tcgtacaaat aaaagagaat tatctttatt catgtaacgc
      601 atccaattga ttttttcatg tacgctcctg gaacgatttt actgacgatt gcttatttaa
      661 ctgcgaatcg ctcattagct tttatcatag ctatccatat tttgaataac gttcttggct
      721 ttgtattata gatacagatg acaaaaaaag ttcaagtatg cggtgtagaa ccaatatcgc
      781 atacttgaac ttttttcaat gagtataaat taaaacttct aattttcaat tcccatcatc
      841 tgatatgccc gaccgtctga acttcttttg aaaaagccaa atgtgattag ataacgtctt
      901 tgttcgacat aatcgccact agaaaatacg ttcagcagta tcgaattaac ctctgtttct
      961 gtataagtca ctccatggtc aaagcgtttc aagacttctt tcaataggat gtatttcttt
     1021 tttgttgaac ttggtacttg ttttactttt ccatcattca tgaatgtgtc taaaactttc
     1081 atcttttctt gctccgtata agtataaacc atcttctttc tccttttgat tctaagaata
     1141 ctccatttat gttagaatat tctggatttt ttattaaagc ggttactctt aagaaagata
     1201 ttatcatagt gctctttttt ttactactac ttataggtag atttattcta atccattatt
     1261 ctcccctacc aataattaaa gaaaaaactg gatcagggtg ttcaaattac atgctaagaa
     1321 aaaagaaccc ttgaatacca agggttcttt ctcagtctga taacaatgat taacgacgga
     1381 tttctttgat acttgtgaaa agtcgttaaa gtattgatat aaaaggattt ttattacctc
     1441 tgactacata ttgactaatt tgttaaaaaa tcgtttttca agaatcctgt aataccagtg
     1501 ctttcaccgt ttttttaact ctctaaagaa ctaaaagcaa tcagaggttt taccttttca
     1561 tttgacgtta agtggcgata aattttcgaa gttgtttggt cagtatggcc taagcgatct
     1621 ttcaagactt cccagctaca gtgttccgct actaagcttg ccattgtatg acgagctaaa
     1681 tgagtatgaa atggcttatt aattcctgag cgattcacgc accgtttgac gtgatcattt
     1741 acatactctc gacgataagg aacaccacgt ttactagaat caagatacac aaacaaatat
     1801 gtttcttcta taataaacct gtaatgttgc ctcatctcac ccaaagcctt aatcaactgt
     1861 tgtagctttt ccataactag aggagttact agaactcgtc tagcagaact aactgtctta
     1921 gtagtttcaa ttcgaaaatc cttactctta cctttaactg ccgtttgctt atagaacaga
     1981 atttcatttc ttttgaaatc tatatctcca ggctgtagag caagcgcctc gctgactcgg
     2041 cagccagtgt aaaatagaac atggaaaagt acagacatcg gtaattcctc atgaacaatg
     2101 ccataattca aaaaagttct caattcatcg acagtaagaa acttgtcctc aatattattt
     2161 atggcttctt ttaaatcagt taccgttaac tgatattttg gcattctca
//
LOCUS       DAYYAR010000231         2171 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000231 DAYYAR010000000
VERSION     DAYYAR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.237"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..525
                     /locus_tag="ACHAT0_002851"
     CDS             7..525
                     /locus_tag="ACHAT0_002851"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8452738.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            653..1336
                     /locus_tag="ACHAT0_002852"
     CDS             653..1336
                     /locus_tag="ACHAT0_002852"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8452739.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1645..1718
                     /locus_tag="ACHAT0_002853"
     tRNA            1645..1718
                     /locus_tag="ACHAT0_002853"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1679..1681,aa:Arg,seq:tct)
     gene            1877..>2171
                     /locus_tag="ACHAT0_002854"
     CDS             1877..>2171
                     /locus_tag="ACHAT0_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8452740.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTS"
BASE COUNT          728 a          339 c          438 g          666 t
ORIGIN      
        1 aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt gattatattt
       61 ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac tccttatatt
      121 attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata tgtacgaccg
      181 aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa ttcaggaaaa
      241 aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt ttatacaaaa
      301 gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat tatcggtagt
      361 cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt tgcatggatt
      421 caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg gaataccttt
      481 gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg ttaaaaacat
      541 aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa acactcatgc
      601 agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta gtatgcgcaa
      661 tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc gtacccagca
      721 aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag cagaagtacc
      781 agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc ttcagcctga
      841 acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg ccacgacaat
      901 cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg agaaagctaa
      961 aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag gtcaagcagc
     1021 agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata gtgcaaaatc
     1081 aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg gcgttttgat
     1141 ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga ttccccgtgg
     1201 caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg atcatcccga
     1261 tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa aggaatcaga
     1321 aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg aatcgagggc
     1381 tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg atacaggaaa
     1441 tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt gataacctta
     1501 gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg gccaaaataa
     1561 ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta attatctata
     1621 attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc atccgccttc
     1681 taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt tagaaactga
     1741 ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca aaggcaaata
     1801 aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa aactcatcag
     1861 aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag ttgcaattga
     1921 atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga caagtagggt
     1981 ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt ttaagaacca
     2041 aggagtgtac aatggactat taggagtggg actactttat ggagcttatc tttcttccgc
     2101 atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat tatatggcag
     2161 tttgactagc g
//
LOCUS       DAYYAR010000232         2160 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000232 DAYYAR010000000
VERSION     DAYYAR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.238"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..950
                     /gene="msrA"
                     /locus_tag="ACHAT0_002855"
     CDS             117..950
                     /gene="msrA"
                     /locus_tag="ACHAT0_002855"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8452741.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1030..2019
                     /locus_tag="ACHAT0_002856"
     CDS             1030..2019
                     /locus_tag="ACHAT0_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8452742.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT          782 a          360 c          434 g          584 t
ORIGIN      
        1 ctctcttttt tatacgaaaa atctgtaggg ggatgaagta atcgcttatt tcctcacttt
       61 atgataaatt acagctaaag tcactagctt gtcttcttcg gaaaggagga gaatctatgg
      121 atgaggaaca aatattcatt caaacacttt ataatctgat tttgaatccg aatacacgag
      181 attgggaacg aaaagtattg attcagacaa agaatgacat aagagaaaat atatccgtta
      241 aagaacagtt atcaaaacta gaagcaacac tacgtccatt agctatccga atgaatctga
      301 cgcctgatgt aatggacttt tatttattga ttacggaagg atttgataag gagcaaaaat
      361 atgacttttc caagcatgcc atgcaagatg ctgactatca agaacgtgca gtatttgcag
      421 ggggatgttt ctggtgtatg gtcgagccgt ttgaatcgaa aaaagggatc ctatctgttt
      481 tgtcaggata cacgggcgga catgttgaaa aaccaaatta tgatcaagta agtggtggtt
      541 acacaggtca tgtggaagct gtggagatca tttatgacac aagagaaata agttatagcg
      601 agttgctgac aatctattgg caaattactg atcccacaga tacatttgga caatttcaag
      661 atagagggaa gcaatatcgt ccagtgattt tttatcaagg cgaacgacaa aaagaattag
      721 cggaacaatc aaaacagaaa ctagacagtt caggaacttt ccaccaacca atcgtcacaa
      781 aaatcgaacc agccggtacg ttttggccag cagaaaacta tcatcagcaa ttttacaaaa
      841 aacaaccgaa acgttataaa aaaatccagc aagcacgtaa tcaatttctg atctatcaac
      901 gagtaaaaaa caaatggcaa aaaaatatac gaaaaaatca ttttgattaa ctccttttct
      961 ttaaaatgta gtaaactaaa aatagaaaaa agaaagcgtt ttaaaaagat ttgatgagga
     1021 ggagaaaaat tgtatattgc agatagtgca cgttatgaaa aaatggaata tcgtaaatcc
     1081 ggtcattcag gtttgaaatt accgatttta tctctcggat tatggcaaaa ttttggtgat
     1141 tatgatccta tccataatca aagagagatt ttaagaggcg cattcgacat gggaattacc
     1201 catttcgacc ttgcgaataa ttatggaggc cctgctggag ctgcagagaa aaactttggg
     1261 cgtattttta gagaagattt tcaagcctac cgtgatgaac tgattatttc tagcaaagca
     1321 ggttatcata tgtgggaagg gccatatggg gaatggggct ctcgtaaatc catcatttca
     1381 agttgtgacc aaagtctcca aagaatgggg ctagattatg ttgacatctt ctatcatcat
     1441 cgcccagacc ctgatacacc tttggaagaa acagcagaag ctttgatgca acttgtaaga
     1501 caaggaaaag cattatatat tggtatatcc aactataatg gagaagatac aaaaaaaatg
     1561 actgaaattt taaaaagaaa ggaagcaccg ttcattattc atcagatgcg ctacaatatg
     1621 ttttctcgtg cactactaga agacgatcta tctccagtat tagaagaaga agggctaggg
     1681 gcaatcacgt tcagtccgct tgctcaaggg ttgctgacaa atcgttatct acatggtatt
     1741 cctgaagatt ctagagcaca tcgtaaggaa ataccattcc tttcagaaga acaagttgga
     1801 tcaactctgg aaaaaatcaa agcactgcaa acaatcgccg tatcacgagg acaatcatta
     1861 gcgcaaatgg cccttgcatg gaatttgcga caaaaatctg ttacaagtgt actggtcgga
     1921 gcaagtcggt taagtcagtt acaagaaagt gttcgaatga tggataattt agatttctca
     1981 ccagaagaag aattgagaat cgaccaaatt ctagaataga aacgatgtaa tcaaactttt
     2041 tatccatata ttaaaaaaaa caaaagatga catcaagaat cgaatgaaga tcgtctgtta
     2101 ttgaacatca tattttttgt cgttcaaaat aaaaatgatc gaaggctagg agtgaatttt
//
LOCUS       DAYYAR010000233         2103 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000233 DAYYAR010000000
VERSION     DAYYAR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.239"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACHAT0_002857"
     CDS             complement(<1..426)
                     /locus_tag="ACHAT0_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8452743.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACHAT0_002858"
     CDS             complement(535..1566)
                     /locus_tag="ACHAT0_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8452744.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          656 a          337 c          361 g          749 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaattata ttgagaatta atcctttttt atataagttg tttgcttatt
     2041 gatggattgg ataaattaaa ccatcacaac gagtggaaat tatatgaaat ttttcataaa
     2101 aat
//
LOCUS       DAYYAR010000234         2090 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000234 DAYYAR010000000
VERSION     DAYYAR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.240"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..607
                     /locus_tag="ACHAT0_002859"
     CDS             <1..607
                     /locus_tag="ACHAT0_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8452745.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELGMICGKLDRQYYSASAISSIVIPPK"
     gene            620..1528
                     /locus_tag="ACHAT0_002860"
     CDS             620..1528
                     /locus_tag="ACHAT0_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8452746.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1528..>2090
                     /locus_tag="ACHAT0_002861"
     CDS             1528..>2090
                     /locus_tag="ACHAT0_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452747.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQA"
BASE COUNT          703 a          307 c          497 g          583 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtatgatttg
      541 tggaaagtta gatcgccagt attatagtgc aagtgcaatt tcttccattg tcataccacc
      601 aaaataggga ggtgatttaa tggcaaagtg gaatgtcata ttaagtacaa cagaacccta
      661 taattacgtg gggatgattc aagttcgaca aggcaataag aatacagagg ttatggaagc
      721 gactatagtt gaaaatggtc ttccctacga tttatcagaa tgtaaggtat attttgaatc
      781 agttgtaggt gggaaatatc cagtccaatt agaaacaaaa attgtggatg ctaaaaaagg
      841 gaaaattaac tatatttttg ataaatattc catgcagtgt ttacatcgac aaacagccaa
      901 tttcattata tttaaaggag aagacttgat tggaacaact caagacttct cttattttgt
      961 cattaatgct gtttcaaaaa cagaaggaga aatgggttct tattggcaat caatcgaaga
     1021 tttaattgcg gatatgacgg actttattaa tgaaaataaa ggcgatttta cggactggat
     1081 gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa caggaacaca gtttccaaga
     1141 ttggagagaa ggacaagaat cagactattt ggcatggttt gaatcaatca aggatatttt
     1201 aaagacgatt gatccaggtg gagtcatgtt agccgaacta acggatgcgc gagtagatat
     1261 tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg gcagatatgg actatttata
     1321 tcagaaattg cgagcaacgc ttttcacgat tgaatacggg gagattgaag tgactgatat
     1381 tttgcaggat gatctctttt cagataatca tgaagtcgaa aaagttggaa ctgtagcatt
     1441 cccgattgaa gaaggagcct tgatcatcgc aaccgttgat gatccaaagc aaaatgtgtt
     1501 cactcttgag aaagttgggg tgatctaatg gctaaaacta aacgaatgat ggaaacggat
     1561 gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac atgcttccgc agttcttggg
     1621 ttagaagaaa taatagcagg agaagcaaca gttttatctg ttaatggaaa aattggagcc
     1681 gtcgtcatta ctaaagaaga tttgggatta gagaatgttc tcacagaatt accctatgca
     1741 agtgaagaag atgacggcat tatcacggca gaaatgtatc aaaaaatttt aaacagtgga
     1801 gaaggtgact acgtgttgcc agttgccact atcgaccgtt tgggtggcat aaaagttggt
     1861 gaattattga cgattgatga gacagggaaa gtctctgcag tcagacaatc tgatgtcaat
     1921 ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa attatactgc tggagaaaac
     1981 atcactattg atgaagatgg aacaattagt tctacaggtg gtagtgggac tggtggtgtc
     2041 aatcagtcat atgtagatca aaaattccaa gaagctgtaa atcaagcaga
//
LOCUS       DAYYAR010000235         2079 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000235 DAYYAR010000000
VERSION     DAYYAR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.241"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..461
                     /locus_tag="ACHAT0_002862"
     CDS             <1..461
                     /locus_tag="ACHAT0_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFM8452748.1"
                     /translation="SEYIDNPLIQEYILGEEYTIDVCLNFDSTPVSIVPRKRIATRGG
                     EILKGQIIKDRELIETTKKMLEKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPISI
                     KSGANSPEYIIKMIDSKKNLRYRENYLDKMIALRYDQAIYLDEKGNVYND"
     gene            454..1149
                     /locus_tag="ACHAT0_002863"
     CDS             454..1149
                     /locus_tag="ACHAT0_002863"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8452749.1"
                     /translation="MIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIFPATFDEI
                     YRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLENADTLLTE
                     LRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPISYLLLLE
                     KFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFYQADKKIR
                     SLWEIKNFLGVIS"
     gene            1146..>2079
                     /locus_tag="ACHAT0_002864"
     CDS             1146..>2079
                     /locus_tag="ACHAT0_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFM8452750.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNI"
BASE COUNT          803 a          250 c          409 g          617 t
ORIGIN      
        1 ttagtgaata tatagataat cctcttattc aagagtatat tttaggtgaa gaatatacta
       61 tagatgtttg cttaaacttt gattcgacac cagtaagtat tgttcctaga aaaagaatag
      121 caactagagg tggggaaata ctgaagggtc agattattaa agatagagaa ctaattgaga
      181 ctacgaaaaa gatgttggaa aaattaaatg caatcggaca tattactatt caatgtataa
      241 aaaacgaaaa gggaatattt tatttagaaa taaatgcaag atacggtgga ggagccccca
      301 ttagcattaa atcaggagct aattcccctg aatatattat aaagatgata gattcaaaaa
      361 aaaatttgag atacagagaa aattatttag ataaaatgat agcactgaga tatgatcaag
      421 cgatttattt agatgaaaaa ggaaatgtat ataatgatta aagcggtgat ctttgattta
      481 gatgacactc ttgtgtctga atatgattat gtaaaaagtg gatacaaatg tgtagctaga
      541 agattagcag tgcaaaaaat tttcccggct acttttgacg aaatatatag agtcctttta
      601 gatctttttg ctgaagatag taaaaatgta tttaatcgtc tatatgacta ttttgaaatt
      661 ccttatagta aaaaagaaat taaagaactg gtaaatgtat atcgagaaca caaaccagac
      721 ataaattttt tagaaaatgc agatacttta ttaacggaat taagaagtag aggatacaag
      781 ctggggatta ttacagatgg gtatgctata acccaacgaa acaaattgga tgctttgaaa
      841 gtagactatt tggtagatta tatttgtatt actgacgaat taggccgtga attgtggaaa
      901 ccacacccta tctcatatct tttattgtta gaaaagttta atttaaaggc acaagagtgt
      961 atatatattg gcgataatga gttgaaagat tttataactg ccaaatcaat aggaatgcaa
     1021 acaattaaaa tagaaagagt aaatggtatt tacagagaaa gcagtttgaa gttcaaaaga
     1081 ttttaccaag ctgataaaaa aatacgttcg ttatgggaga taaaaaattt tttgggagtg
     1141 atttcatgaa aagtaaaaga attctattag cttctccaca tatgagtgaa gaaggatatg
     1201 aaaaagagta tgtaaaagaa gcatttgata caaattgggt tgctccttta ggtgcgaatg
     1261 tgaatggatt tgagaaagaa ttagcagaat acgtgggagc tagtcatgct gccgcattat
     1321 cttctggaac agcagctata catttagctt tgaaagcggc tggggtcggt aaagatgaca
     1381 tagtgatttg tcagtccttg acgttctcag ctactgctaa tccaattctc tatcaaggag
     1441 ctatacctgt atttgttgat agtgattacg aaacttggaa tatgaaacct gaacatttgc
     1501 gaacagcact agaaaaatat cctaacacaa aggctgtatt agtagtacat ttgtacggtt
     1561 tatcagctaa tttagacgaa attactaaaa tatgcaaaga atacgatgtt actctaattg
     1621 aagatgctgc agaatcatta ggaacaacat ataaaggaaa atatactggt acatttggcg
     1681 actatggaat ttattctttt aatggaaata agataatcac aacttcaggt ggcggaatgc
     1741 ttgtatccaa tgacaaagaa aaggtggaaa aagtgagatt ttgggctacg caatctcgtg
     1801 agaaggcacg ccattatcaa cacaatgaaa ttggctataa ttatcgaatg agcaatatcg
     1861 ttgctggtat cggtcgagga caattgaaag tattaaatga aagaattgag aaaaaacgat
     1921 atattcatag tttttataaa aaagaattgt ctaaattgtc aaatatacaa ttcatgccag
     1981 aaaataattg gaatgaaccg aattattggt taagtgtcat aacaataaag ggaataaatg
     2041 cgaatcatct tattgatgag ttagacaaaa ataatatag
//
LOCUS       DAYYAR010000236         2059 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000236 DAYYAR010000000
VERSION     DAYYAR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.242"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..417)
                     /locus_tag="ACHAT0_002865"
     CDS             complement(130..417)
                     /locus_tag="ACHAT0_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8452751.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(588..1940)
                     /locus_tag="ACHAT0_002866"
     CDS             complement(588..1940)
                     /locus_tag="ACHAT0_002866"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8452752.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT          561 a          410 c          356 g          732 t
ORIGIN      
        1 aaaaaagggg ttgacaaagc ttgtcataac ccctttttct gattaaaaga tagtaaagag
       61 acagtcatcg gtaattgtga tgattttttg tcatcactta aggatgagcg gccttttcaa
      121 aaactgtctt taaagatcat catcagtttc tagcacatat ggctgacctt catatgtgta
      181 ctctgtattc aatgtgccgt cattttcatc tgagtagatg cgtctactga aaggaaaacg
      241 atgagaaaca ttatctttgg cgtcatttgc tggcatcgtg taatattcct cttgcgtttc
      301 attcaactga gagagtactt gatctgccaa ctcatcaata ttgatcgtta atttcttcga
      361 agcactttta taatcttcta aaaatgcatc ttgttggaca aaatattctt tattcatctt
      421 ctttctcacc tttcttttca aaatcaaata aagctgcttg ttcttttatg taacagcttt
      481 atctttgctg cttattgtga caaaaaattc gaagaaagac aactaattgt acttttcaca
      541 aaaaaagaca gagggtcgtg acaaaattgt gtcacaaccc tttttcttta ttgtgcagtt
      601 tcgatttcgc ctagcgctgc ttgtgcaagt aggttcaagt aattccatgg gcggtcaaaa
      661 tgtggttgga agaagaaatc tgaaagtgcg agatcttcta cagtcatttt attttgaaca
      721 gctaaagaca tggtatttgc tgattgtgta atgtcgtatt tggacatgaa ctgagcacct
      781 acgatacgat tcgttccttt ttcatagact agctccatca ttactttttc agtagttggc
      841 atgaactctg gacgataatt atcttcaaat acagtcgctt ggacttctag atcgttcaaa
      901 gttgcgcttt ctttggtcac acctgtcgaa ccgatcgtcc agccaaatag gtaaagtcct
      961 gaagttcctt gagtaccacg atacgccatt ttatgatctg tcaagttatt tcccacaagc
     1021 aaaccttgac gaacggcatt tgttgccaaa ggaatatagt tttgcgtgcc acttggattg
     1081 tagttgacta ctgcagagtc tccagctgca aaaatgtctg gattacttgt ctgcatgtat
     1141 tcattcactt tgattgcccc gtttggcagt gtatctactt ttccttcgat cagttttgta
     1201 tttggacgga agcccacaca taaaatgacc atatctgtat caaatgtatc tgttggtgta
     1261 gctacttgtt tcacttttcc ttcttcatct gtgataaatt cagtaacatt ttcgcctagc
     1321 gcaagagtaa caccgcggtc tgtcaattct ttttctaaga tatctgtgaa tggtttatct
     1381 agatatttat tcaagatgcg atctaatcca tcaatcaatg tcacttgttt tccagattcg
     1441 acaaaagctt ctactaattc aataccgata tatccaccac caacaattac tacacgtttt
     1501 gcttctttcg cttgtgcaat aatttcattt gcttgattgt agtttttgca taggagtaca
     1561 tttttcgcat cgattccact aatcggtggg ataataggcc atgaaccagt tgtcatcact
     1621 aatttatcat aagaaaccgt ttctgtttct cctgttttca gatcttttgc cgttactgtt
     1681 ttattttctg tatcgacgtc tgttacatcg tgttccattt taactgttgc tcctaatgaa
     1741 gataattctt caggatttga gtagaacaag cctgctgggt cctttactac tccgccgaca
     1801 taaagtgcga taccgcaaga taagaaagaa atattgtcat ttctttcata tactgtcact
     1861 tctgcttctg gatgattttt taaaatactt tttacagctg cagtacctgc atgagtacag
     1921 ccaattacta ctactttcat ttatggtccc tccgattaac attttgtcct tcgacaaaac
     1981 tactataaca gaaataaaac ggatacaaca gtttaatgct tgtgaattca caaaggtttg
     2041 tttgtagttt cattccttt
//
LOCUS       DAYYAR010000237         2027 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000237 DAYYAR010000000
VERSION     DAYYAR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.243"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(355..969)
                     /locus_tag="ACHAT0_002867"
     CDS             complement(355..969)
                     /locus_tag="ACHAT0_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8452753.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1575..1952)
                     /locus_tag="ACHAT0_002868"
     CDS             complement(1575..1952)
                     /locus_tag="ACHAT0_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452754.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          668 a          402 c          311 g          646 t
ORIGIN      
        1 caaatgtact ataattactt tgtaaagagt tttggggaag actctttcat caacgtgtat
       61 taagagacaa gaaagaatcc atatggattc tttcttgtct ctttttccac tactttttat
      121 ttttctgaca ctacatattc ttataactca tttctcataa ctgtgaaaca acttataaaa
      181 ataacaaact ttaaacaaaa caagcttaaa atcaaagatg atccatctta aaataactaa
      241 aacaaaaaaa gtagtcgttg ttaatcctca ttaataatcg ttttcttttt ttcaaacgct
      301 ccatttttat gtggaacatt atttttttaa ggaactttgt caacatgtgg ataactattt
      361 tccacttgtg tcctggacta gctgttttgc tgtaaaatac aaaacttgct gcagcaaaaa
      421 gatcgttgca agagaggctg ttccgcagat cgctggatct agatttggca aagtcaagac
      481 tgcatcatgt aaattggcgt ggttgtccaa aaccaaatca gcaaattcaa ataatcgcaa
      541 accactgcta tgttgcgatt tgatacttcg tgacaaatca aatccagtaa caatgattaa
      601 tggatgacca ttttctttga tccactgagc tagttcgatg attgccgggt ttcttccttc
      661 attcgaaaga agaagaaaaa tgtcttctga tttgacagaa agctgatcaa taattgcctg
      721 tcctgcacct tctagcgttt catagacacc tccggctgga ttttcaatca atatacttgg
      781 tacaagatcc ggggtttgat cccaaaattc aaatgctaca ccacttagtg tgcggctagc
      841 atatatctgc aatctaccac cttgattaat ttgtttcgcg atccaagtgc cagcttgaag
      901 catcagcggc tcttctatcc ttaatttctc ttccaattgg tgataatagt caaaataaca
      961 taaactcatt tatctctccc gcctagctat acttgacatg aatagaaaca gctgcctaat
     1021 ctaaggaagt acataaaaaa acagtagtta gacagtaaca catgtggtct ttgctgtttc
     1081 aaacacaaaa aaggcattga acgaatacgc ccaatacctt gacatagcaa ataaaacaac
     1141 gtgcagacca tggaaaatat ctacataggg gtagaatttt caccctagat taggtacaat
     1201 aaattaggtc gatgcactgc attgactatg gcaaaatgat cggtcagggt gtatgtgtgt
     1261 gactggtcat tccctgattc ttagcgccaa ctaagggtca gggagccttt ttttattcag
     1321 ctgtttctat ctgaatttat tgtaagtaat ctcttatcca agtgcaagca agaaaataca
     1381 aaaaaacatt ggtatgattt atatatatat aaataacata aaaaaaggaa tggcttatcc
     1441 cgatacaaca agcattcctt taaaaaagat tcttttatat aagtgaaaaa agcttttttc
     1501 caccaattaa ataaatctct catctcaata gtttatccat tattttatct ttttttgcgt
     1561 atacctacta aatattaccg tcttcttctc gttcttctac tttgttcgag agaatctgta
     1621 ctagaaaaag tagttgtatg tctacctttt ctgcctatcc gagactttct tttcatatat
     1681 gggatataaa tgattagata cagtacagca aaaaataaag caaatacgca agcttcccaa
     1741 ctacctaacg ccaattcaac catcaaggta gcaaccccaa cggatgctgc ataccagata
     1801 aaaatcagaa acgtttcttt ttttctgacc atgcgaaaca tgataaatag actaataagc
     1861 aatagtggca acacaaatat ccaattgctt ccttccatca gccacttgag tatatcaagc
     1921 agcgactgtg caacagcgac taattttccc atagtgaaca cacacctttt tttataatta
     1981 aaaaattcgc ctttttattc tactatgaat cttctggctt ggctttc
//
LOCUS       DAYYAR010000238         2004 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000238 DAYYAR010000000
VERSION     DAYYAR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.244"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1622
                     /locus_tag="ACHAT0_002869"
     CDS             156..1622
                     /locus_tag="ACHAT0_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8452755.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          632 a          336 c          406 g          630 t
ORIGIN      
        1 gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat taaataaaag
       61 aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata tgattacatc
      121 tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa ttacgctggt
      181 ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta gttaataact
      241 atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc atttatttta
      301 ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt aaaggaggcg
      361 tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat gccgcttgga
      421 cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt ctgattgctg
      481 gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta caaatggtag
      541 cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa ggactttcta
      601 ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg atcttattta
      661 ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc acaccacata
      721 tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca accgtttcga
      781 tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac tctacaagaa
      841 atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta gggatttgtg
      901 ctgtattagg ctcagtcgca atgggtatgc tgtttgctag tgataatatt cctaaagact
      961 tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat ggtatcggta
     1021 actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc gcattagcct
     1081 tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa tttgtaccaa
     1141 actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt ctgacaggta
     1201 tactagtagg aatcattatt ttgttgccga tttttggtat caaagatatg aatgaattag
     1261 taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta tgggtgttct
     1321 tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat aaatttgtta
     1381 aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact gcgtttgcat
     1441 gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca tggtggttcc
     1501 aattatttac gaatatcatc acaccaatcg tcttgatctt attaggtatg atcttgccag
     1561 caattgctcg tcgagacaaa aacaaagaaa tcactgaacc agaaacagtg gttcagcctt
     1621 aaaatagaaa tacttgttac aagaggttgt gacagaagtg gtcagcttca agaaataaga
     1681 aggaatttac gaaaattgct tctcaaattt ttgtgaagtt tcggcttgtt tttcgaagaa
     1741 gctacttctg gaacaccgtg aatcagaggt tgtgaaaaag ctgtcctgca tcaagtaata
     1801 agaacagcca aacaaaaata gcccctcata tttttcgttt ggctgggctt attaccgcag
     1861 atgcaagctt tggaacaccg tttaaggaag aggttgtaaa atcagcgatt tgacctgagt
     1921 attagagaaa caaacggaaa aatagctcct catattttgc cgctttttga gctaatgctg
     1981 aaggtcagct gattgaacac catt
//
LOCUS       DAYYAR010000239         1979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000239 DAYYAR010000000
VERSION     DAYYAR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.245"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..367
                     /locus_tag="ACHAT0_002870"
     CDS             <1..367
                     /locus_tag="ACHAT0_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8452756.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..749
                     /locus_tag="ACHAT0_002871"
                     /pseudo
     CDS             567..749
                     /locus_tag="ACHAT0_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1330..>1979
                     /locus_tag="ACHAT0_002872"
     CDS             1330..>1979
                     /locus_tag="ACHAT0_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8452757.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVDDK
                     VHPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQ
                     LFDQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIA
                     CDILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          657 a          305 c          461 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggtttaact
      661 tggggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcgttaaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacga tcgtgttaga ttgtgaaaac cgatagctga atgaaataaa cagcgggtgc
     1021 gccctttcca tttcggcagg aggaggctca aggggttcgg ggaatgaaat tccctgaggt
     1081 ctttttattt ttttaggagg agtgctataa tcaaagaaaa cgagaaaaaa gagcaaaata
     1141 aaagccgagc agtgcgctaa cactctcgac tatttacgga aactacttca cctagctccg
     1201 taactcaggt tcattatacg aatattttta ggtttcttca agctagagat ttttggatat
     1261 gaatttttag ggttacgtga actctggaaa ttctttccaa aagtctcttt tttcgtctgg
     1321 aggacgaaaa tggcaaatga aaatcagttg agtgaaattc aatcaaaaat tgaaggtcga
     1381 ggggcagaaa aaagaacaac gataaatcag ctcgaaaaac ttaaccgtat ttctaacagg
     1441 cggttaagtg ttgacgataa ggtacacccc ctaaaattaa gttggtcaat cgatcgaatt
     1501 acaatcgtcg gaaaactgaa tgataccata gcttattgga cgcaaaatgg agttgtgttt
     1561 gtcgattttg agcaattaat gcgtttgaat gaaaatcaag ggtatttgaa gtcggttggt
     1621 acaaatggtt ggcaattatt tgaccaatat ggagaagata tcgcctatat cgaaatgtta
     1681 aaatttcacc ctgacaaagg tcgtattgat tttaatccaa ataaaattgg cgagttttta
     1741 aaggggtcga tgaaacaatt cattcatgat ttgtttctgg atgcgcattt ctcacgtgca
     1801 gatattgctt gcgatattct cggtgttcct gatgatttta ttacccagta tcgaatcgtt
     1861 gaccctgtgt cttttaagcc aatttatggc cgttctggca aattagaaac agcgtactgg
     1921 ggtagtcgtg ccagcgagcg acaagtgcgg atgtacaaca aaaagcttga acaggaacg
//
LOCUS       DAYYAR010000240         1956 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000240 DAYYAR010000000
VERSION     DAYYAR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.246"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="ACHAT0_002873"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="ACHAT0_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8452758.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="ACHAT0_002874"
     CDS             853..1041
                     /locus_tag="ACHAT0_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8452759.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="ACHAT0_002875"
     CDS             1054..1611
                     /locus_tag="ACHAT0_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8452760.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="ACHAT0_002876"
     CDS             1643..1885
                     /locus_tag="ACHAT0_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8452761.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          716 a          305 c          404 g          531 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttc
//
LOCUS       DAYYAR010000241         1880 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000241 DAYYAR010000000
VERSION     DAYYAR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.247"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..553
                     /locus_tag="ACHAT0_002877"
     CDS             <1..553
                     /locus_tag="ACHAT0_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452762.1"
                     /translation="IKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGY
                     GIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSST
                     LLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRL
                     SEEKILLPENWAELRKLVEYGII"
     gene            540..>1880
                     /locus_tag="ACHAT0_002878"
     CDS             540..>1880
                     /locus_tag="ACHAT0_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8452763.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYV"
BASE COUNT          626 a          242 c          287 g          725 t
ORIGIN      
        1 tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca tacaatcaat
       61 ctttgggata gaagaagttt ttgtcggaga taaatgtatc ttttttgatc aacctttatt
      121 atctgatggt tatggcatag atgaaacaga tattttcgct aaagtaaata gtatattttc
      181 tggaaaaaaa gaaactttaa ttaagcttca tcctagagca aggacaaatc gttatggtga
      241 aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa atgacttctc
      301 ttcaacactt ttgattagtc ctatatctac aatttctttt tcaccatcta tgatgtttga
      361 aaagacgatc aactctatta tgttggctaa attaatttta aatgaatata cagcattatc
      421 tgaagataaa aaagaaacat tgacaaatat tgttcttttc tgtgaacatt ataaccgtct
      481 atcagaagaa aaaattttac tcccagaaaa ctgggcagaa ttaagaaagt tggtagagta
      541 tgggattatt taacacactt tccaagtcaa aattagttaa gtccagtttc tggtatacta
      601 tagggaattt ttttattaag ggaatttcat tcataactat tcctattttt gtaaatatta
      661 ctactcaagc agaatatggt ttgattaata atttcacttc atttgtatca attttttcga
      721 ttcttgttgg gctaagccta aattcggcaa ttaataatgc aagttttgac tataaagatg
      781 acataaatga atttatgtca tcggtacttt ctctagctac actttcgtta attgtgttct
      841 tattaatagg gaacatatat tttttgtttt caaatgcttt ttttgatatg aaccaacttg
      901 tatttaattt gatgttatta caaagttttg ctagttttgt aattagttat atccagtctt
      961 attttacgat taactttaac cattttaaat atttgatttt atcttttata tctacttttt
     1021 tgaatattgc aatctctgta gctttgatgc agacattata ttttgatgat aaatatgttg
     1081 gcagagtagt cggcggaaca ctgtcatttg tgttgatagg tttagtaatt tatttaagaa
     1141 taatgataaa gggtgctcat ttttttgata aaaaacagtg gagatacgct ttaaaaatct
     1201 ctttgccgct aatcccacat tccttagcaa atatagtttt agcacaattc gatagaatta
     1261 tgattaattc ttatttaggt cctgctgcgg ggggaatata ttcctacatt agtaacatag
     1321 gtattattct aagcgtaata tggatgtcta ctaataacgc ctgggttcca tggttctatg
     1381 gtgagatgag taaaaataat gaaaaaaaag tgaagaaagt tagtaactat tatttagtag
     1441 tctttacttt gattacgatg attgtaatgg tagtttcgat tgatttagca aaatttatgg
     1501 ctccgaaaga ctattattct gggttatctt tagtggttcc gataacctta ggctatttct
     1561 ttcagttttt atatagttta ccagttaatg ctgaatttta tgagaaaaaa acatcgttta
     1621 ttgcgattgg tacagtagca agtgctatag ttaatatact attaaacatg attttcatac
     1681 ctaggttagg aataattgct gctggataca caaccgtatt tacgtatttt ttacttttta
     1741 tttttcatta taatatcgct aaaaaaatta cgaataaaca attatttgac acaaaaaaaa
     1801 tatgtatcat aataattttt gtatcaattt tttctttgat acttcatatt ttaatcaatc
     1861 aaatgttgat tcgttacgtt
//
LOCUS       DAYYAR010000242         1877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000242 DAYYAR010000000
VERSION     DAYYAR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.248"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..945
                     /locus_tag="ACHAT0_002879"
     CDS             <1..945
                     /locus_tag="ACHAT0_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8452764.1"
                     /translation="INDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNVAANMKNNY
                     NGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRA
                     YIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKE
                     KLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHV
                     AGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNF
                     PSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1135..1208
                     /locus_tag="ACHAT0_002880"
     tRNA            1135..1208
                     /locus_tag="ACHAT0_002880"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1169..1171,aa:Ile,seq:cat)
BASE COUNT          631 a          319 c          359 g          568 t
ORIGIN      
        1 attaatgacg agtataattt agcgccgaat caaggagact ccagattagc aattcctaac
       61 aaaattattt tgcacgaaac tggaatagat gcaccagcaa gaaacgtagc cgccaacatg
      121 aaaaataatt ataacggaag caattcttat actacagatg ttattggtga cggtgggatt
      181 gtttaccgtg tgggtgagca aggatatgtt tcgtggggag ctggtaacgc taatccttat
      241 gcgcctgtac agattgaatt acagcgcaca tatgataaag cattgtttga aaaaaactat
      301 cgagcttata ttgaatatac aagagatagt gcaaaaaaat atggaattcc attgactctt
      361 gatcaaggaa cttctttatt tacaaaagga atcatttctc atttgtgggt gacaaattat
      421 gtttggggga accacacaga tccatatggt tacttatcgc aaatgggagt tagcaaagaa
      481 aagcttgctt atgatttagc tcatggattt accgatgaaa atccaacaac ttctgaaaac
      541 aagcctgtca ttgatccaac acgagctggt gcagctaatc ctacgctgac agatggaaca
      601 aatcacgccc acattgatca gtttggggaa atcgaaaacg caaacttgca tgtggctgga
      661 tggcacattg ctaactataa atacgagtat attttcatta tggactacaa tactgggaaa
      721 gaattagctc gagtaagagc tgatggaatt tatagaccag atgtaaacca agcttataat
      781 acttctggaa atgttggtta tcatgtatct ttcaatatgc gtaattttcc tagtaagaaa
      841 gtctatgtaa tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa
      901 gatttccatg acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt
      961 gaggagcagt acataactat attgacaact ataaaaatta ttcgataaaa tagtgatgtt
     1021 atcgcatatc ttcactatca cccataaata gtcacactcc aagctatgcg ataacaggtt
     1081 tgttgccaca cattctactg gttgattgtt tatggcttta tgtggcaaca accagtaccc
     1141 ttagctcagt tggtcagagc agacggctca taaccgtccg gtcgtaggtt cgagtcctac
     1201 agggtacatt aacgtagcca tttgaatcgt tctgtgttag aatttttttg aagagtatta
     1261 tacaagctaa agcttttctt cattgccact caaatgagtg gcttttttat gtatcctttt
     1321 atggattaat gaaaggatgt ttcacatagt tatacttctg tatatttgaa aagttttact
     1381 ttgattttta aatagaaaga catttgggtt aaattgtgag ataataataa agaagagttt
     1441 aaagcgcacc ccaaaccact tccccataag tgtgttacgc tttaaactct tttatatttg
     1501 aagccattaa aaagcatacc atatttttga aaaaaagtga gaaaaaaggc ttataattgg
     1561 agtggtagtt aattagtgac ttatttttga ttttatagca ctgatactat aaaatataga
     1621 tatcatcata ttacacaatc ttaatactaa cttaaaaaat atctcctttc acaagtatgg
     1681 tgataaaatt cgttccgggc taccttttta ggtagcctac tttaatcttt atacctttct
     1741 ggatcaacga aagtatactt tatatagtca taacgccgat gatcgctacg tgcgtccggc
     1801 acgtcagtca tgaatcggct acactagact agacagaaaa aataaggtgt gtagaataat
     1861 aaaaaacaca ctggagg
//
LOCUS       DAYYAR010000243         1854 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000243 DAYYAR010000000
VERSION     DAYYAR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.249"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHAT0_002881"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHAT0_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM8452765.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACHAT0_002882"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACHAT0_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8452766.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          551 a          329 c          299 g          675 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcga
//
LOCUS       DAYYAR010000244         1845 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000244 DAYYAR010000000
VERSION     DAYYAR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.250"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..709
                     /locus_tag="ACHAT0_002883"
     CDS             143..709
                     /locus_tag="ACHAT0_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8452767.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            854..941
                     /locus_tag="ACHAT0_002884"
     tRNA            854..941
                     /locus_tag="ACHAT0_002884"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:886..888,aa:Ser,seq:cga)
     gene            1275..>1845
                     /locus_tag="ACHAT0_002885"
     CDS             1275..>1845
                     /locus_tag="ACHAT0_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM8452768.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          645 a          277 c          356 g          567 t
ORIGIN      
        1 ttttttaatt ttgcaaaaaa aagttaaaga agactgaaaa aaatttaaaa tataacgttt
       61 tgagtaaaat gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac atatactgca
      121 gtaaagaagt gggggagttt taatgagaag aaaagtctat accaaggatc aaattctaaa
      181 agctgcttat gatgttgttg caaaggaagg gtttaaagga tttactgcga gaaatattgc
      241 taaaaagatg gggatttcga cacagccgat ttatctggaa ttcaaaaaca tggaagattt
      301 aaaaaatacg ttgctggatt ctatctttga acaactaaaa gaagatgttt tccctattga
      361 acgtactggt gataaaatca ttgatctcgg tttgaactat atctactttg caaaaaagca
      421 tcatagtctt tacatggcat tgtacattga tgaacatggg gacggaaaga aaatgcatga
      481 tttttcatac aattatttcc gtgaaatagt taaagaagat gaaagataca atcaattatc
      541 ggatgaatat gtcgatgctc tgcatactgg tacatgggta gcagtgactg gcttagcgtc
      601 attgatgtca tcaaacatca tgcatccaac agaagaacaa atcattgcac tgatcaaacg
      661 gacaattgac tcgattttag cattagataa tcctgaaata atcagatagt gttctatcag
      721 tatgaatttg atcggtaagt ccgttggctg caaactgtca atggactttt ttgctaaaaa
      781 aagcgatttc tgttatttat ctattagaaa taggcttgac atttcgtgaa aaaagtttaa
      841 aaatagtact tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac
      901 cggctaatac cggcgcgcag gttcaaatcc tgtaccttcc ttatatcaac gattagaggt
      961 tgtgaagaag ctgtcctgca tcaagtaata agaacggcca aacaaaaata gcttttccta
     1021 ttttttcgtt tgattgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg
     1081 aataaggggc tgtgacaaga cttttgtcac agccccttat tgttgtactt ttaacctaca
     1141 atattttttg attccccctg agtactattt tttataaatg ctagtaaatc aatcttcttt
     1201 ggcctttttt tgataaaaaa aatactagat gtaagataaa agtgtaaaaa cgatttgttt
     1261 taggagggga aacgatgtgt accgcaatca cttatgcaac gaaagaccat tattttggaa
     1321 ggaattttga ttatgagatg tcttataatg aagtggtcac tattactcca agaaattatc
     1381 gtttcgattt tagaaaggta aaaaatttag ataaacatta tgcaatgata ggaatagcag
     1441 ctggggtatc caactatcca ctctattatg aggctacaaa tgaaaaaggg ttgagtatgg
     1501 caggactgaa ttttcctggc aatgctgatt ataaagaatt acaagaagga aaagataacg
     1561 ttgcaccatt tgagtttatt ccatggatac tagggcaatg ctcaaccata gacgaagcta
     1621 aagaattatt ggctactatc aatctagtca atatcgattt tagtgaaaaa ctacccttgt
     1681 cacctttaca ctggttatta gctgataaag aaaaatcgat agtaattgaa agtatgaaag
     1741 atggacttca tttatatgat aatcctgtcg gtgtgttgac aaataatccg ccatttgatt
     1801 atcaattatt taatctgaac aattatcgct cgctatcaaa tggaa
//
LOCUS       DAYYAR010000245         1811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000245 DAYYAR010000000
VERSION     DAYYAR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.251"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..308)
                     /locus_tag="ACHAT0_002886"
     CDS             complement(<1..308)
                     /locus_tag="ACHAT0_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8452769.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     "
     gene            500..1795
                     /locus_tag="ACHAT0_002887"
     CDS             500..1795
                     /locus_tag="ACHAT0_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8452770.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          562 a          364 c          333 g          552 t
ORIGIN      
        1 ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc aatgtttact
       61 ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt gtcatgtttc
      121 tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc ttgaagcatt
      181 ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc tacagattcg
      241 ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt tcttttcttt
      301 ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc gtgaagcaac
      361 cacccgccta ggattgaggc tctatacttt ttgatgttgg tgaacttcac caacatcttt
      421 tttgtataaa aaaatataga aaagacaccc tgaaatcctt tagaattaat ttggctacca
      481 attcatagga ggattcaaga tgtctcatac tcagcttatc aaagataccc taaatattct
      541 agacctaaat atctatttcg aggaaaattg tttaactaaa gaaaaataca aagggcaaat
      601 ttgcttgatt tacaaaggaa ctcttctgta caagccagaa gagtgttccc attgtctttg
      661 tgtcgtcccc tctaggatca ttcgttgggg gacgacaacc gttcggttgt tactgaatga
      721 tgtgtctgag tatcgcactt atctggagct gaagaaacaa cgatttaaat gtaagtcttg
      781 ccaaagaaca ttcgtagcgg atacctctgt tgctgaaaaa cactgtttca ttagtcaaaa
      841 agttcgttgg tctgtcattg ctcgcttaaa agagaatact tccatgacgg aaatcgctcg
      901 tcagaaaaat atttccactt cttcggtcta tcgtgtcatg aagcgattct accgcccatt
      961 gaatcctttc aaacagactt tgccaaaggt cttatgcttt gatgaattca aatcggttcg
     1021 aagtgttgcc agtgcaatga gttttatcat gatggacgga caaagccatg cattattgga
     1081 tattgtcgaa aatcgacgct tgccttattt agaacgctat ttttctcggt tttcattagc
     1141 gactcgtgaa gccgttcaat tgattgttat tgatatgtat gcgccttatg tttcgttggt
     1201 gaaaaagctt ttcccgaatg ctcagttgat catcgatcgt ttccacatcg tccaacatat
     1261 tggcagaact ttcttgaatc atcgagtgaa agaaaccacg gtacgactta aaggaaattc
     1321 ccactctcaa cgagggttag ggaaaaaatt gaagcgctat tggaaactcc tacaaaaaga
     1381 agaagcaaag ttaaactatg aaaaacgggt ttggcgtgcc agttttaaag attatttgac
     1441 tgaaagtgag attgccgatc gactgttagc tgcttgtccc aacttacgcc aaggctatca
     1501 gttataccaa gacattcttt atgcggtaaa gaaaagagat cagtcactat ttgaggattg
     1561 tttaactaga gacattagcg gtctgccaga aacctataca accactttac gaacgtttaa
     1621 aaagtttctg ccacagatcc aaaatgcgct tcactacagc tattcaaacg gaccattgga
     1681 atgcttaaac aaccacatca aagtattaaa acgcaatgcg tacggctttc gcaactttta
     1741 caatttcaaa ctacgaatta tgattcgtca tggcaaaaca tttttaacca aataaacaga
     1801 acagaaagag g
//
LOCUS       DAYYAR010000246         1800 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000246 DAYYAR010000000
VERSION     DAYYAR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.252"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1581
                     /locus_tag="ACHAT0_002888"
     CDS             <1..1581
                     /locus_tag="ACHAT0_002888"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HFM8452771.1"
                     /translation="INNSDSVIKDETSFENQLLNGKLLGKIINFSKDNEDYPLNVFWE
                     NTDGSRVLFGEIIGQNNSMYGVAYRIEKDNFYKKFIDEGSKNYVGYDDFINQTKDIIF
                     VPYLLLQKIIETKKDINEGIENISDSKISPVSSIITEQTQIDISELEDKHAIQQNDHF
                     SEHNEFIERFNQLTIRSKLHYSKRDLWNFHTAMLGDSLVVLAGLSGTGKSQLVTSYAK
                     ALQLSDTQIKFISVRPFWEDDSDLLGYADTVNSVYRPGDSGLIDALLEASRHKENIYM
                     VCFDEMNLARVEHYFSQFLSVLEMEPNTRKIKLYNEDLENKLYNSSTYPSTIEVGQNI
                     LFVGTINTDESTHQFSDKVLDRSNIISLELVPFHEISEVNDYIQSDQKKRNQLKYDDF
                     KNFKNSNPTNGLTQKEKEMFWKLHLAINSKDKNIGIGWRILKQIDEYLKNLPVQNDLS
                     RKEAIDIQLVQRVLSKIRGSDEQLSELVGKWNENDLKQVGEFEQIINEYPESSDFKYT
                     KKILLQKARELNLHGFTL"
     gene            1565..>1800
                     /locus_tag="ACHAT0_002889"
     CDS             1565..>1800
                     /locus_tag="ACHAT0_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452772.1"
                     /translation="MDLPFSLKLNFGYKSEEQEYELLFFQDEASLLEYTPVKVKEFSN
                     LSFTFNSIDKHAKLYIPELDDYLIENQVKEENGD"
BASE COUNT          718 a          235 c          289 g          558 t
ORIGIN      
        1 ataaataaca gcgattctgt aatcaaagac gagacaagtt ttgaaaacca attactaaac
       61 ggcaaactac tcggaaaaat tataaatttc tcaaaagaca atgaagatta ccctttaaat
      121 gttttttggg agaacactga tggatcaaga gttttatttg gtgaaataat cggacaaaac
      181 aattcaatgt acggtgtagc atataggata gaaaaagata atttttacaa aaagtttatt
      241 gatgaaggta gtaaaaacta tgtaggttac gatgatttta taaatcaaac aaaagatata
      301 atttttgtgc cctatctatt acttcaaaaa attatagaaa ctaaaaaaga tattaacgaa
      361 ggcatagaaa atatctctga ttccaaaata tctccagtaa gttcaattat tactgaacaa
      421 acgcaaatag atatatctga gttagaagat aaacacgcta ttcaacaaaa tgatcacttc
      481 tctgaacata atgaatttat agaaagattt aatcagctca caattcgtag taaattacat
      541 tactcaaaac gagacttatg gaattttcat actgctatgt tgggtgatag tttggtggta
      601 ttagctggac taagtggtac cggtaaatca caattagtaa catcctatgc aaaagcactt
      661 caattatcag atacacaaat aaagtttatt tctgtgagac ccttttggga ggatgattct
      721 gatttattgg gctatgctga tactgtaaat tctgtctatc gtccaggaga tagcggtcta
      781 attgatgcac tattagaagc atcaagacat aaagaaaata tttatatggt ttgttttgat
      841 gagatgaatt tagctcgggt tgaacattat ttctcccaat ttctttcagt tttagaaatg
      901 gagccaaata ctaggaaaat caaactgtat aacgaagatc tagaaaataa attatataac
      961 tcttcaacct atccttctac aattgaagta ggacaaaaca tattatttgt aggaacaata
     1021 aatacagatg aatctactca tcaattctca gataaagttt tggatagatc aaacattatt
     1081 tcacttgagt tagtaccatt ccatgaaatt agtgaagtaa atgactatat tcaatcagat
     1141 caaaagaaac gtaatcaact taaatatgat gatttcaaaa attttaaaaa ctctaaccct
     1201 actaatggtt tgacccagaa agaaaaagaa atgttttgga aactccattt agcaattaat
     1261 tctaaagaca aaaatatcgg tatcggttgg agaatattaa aacaaattga tgaatattta
     1321 aaaaatcttc cagtgcaaaa tgacttatca agaaaagaag ctattgatat tcagcttgta
     1381 cagagagtat taagtaaaat cagaggttct gatgaacaat tatcagaatt agttggtaaa
     1441 tggaatgaga atgatttaaa acaagtgggt gaatttgaac aaataattaa tgaatatcct
     1501 gagtcttcag attttaaata tactaaaaaa attcttttgc aaaaagctag ggagttgaat
     1561 ttacatggat ttacccttta gtctgaaatt aaactttggt tataaaagtg aagaacaaga
     1621 atatgaactt ttattttttc aagatgaggc atcgttatta gagtatacac cggttaaggt
     1681 aaaagaattt tctaatctaa gctttacatt taacagtata gacaaacacg caaaactata
     1741 tataccagaa ttagatgatt atttgattga aaatcaagta aaagaggaaa atggagatat
//
LOCUS       DAYYAR010000247         1793 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000247 DAYYAR010000000
VERSION     DAYYAR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.253"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHAT0_002890"
     CDS             131..505
                     /locus_tag="ACHAT0_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8452773.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHAT0_002891"
     CDS             complement(619..1248)
                     /locus_tag="ACHAT0_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8452774.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHAT0_002892"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHAT0_002892"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACHAT0_002893"
     tRNA            1668..1741
                     /locus_tag="ACHAT0_002893"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
BASE COUNT          621 a          263 c          313 g          596 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt ttt
//
LOCUS       DAYYAR010000248         1773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000248 DAYYAR010000000
VERSION     DAYYAR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.254"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHAT0_002894"
     CDS             <1..1604
                     /locus_tag="ACHAT0_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8452775.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          641 a          285 c          359 g          488 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct
     1681 ttttacttgt tcacatttta taacggagta tttaagaaag ctattgaata atgattcttc
     1741 catctttttc attcatttac cggttctgtt gca
//
LOCUS       DAYYAR010000249         1731 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000249 DAYYAR010000000
VERSION     DAYYAR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.255"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..>1731
                     /locus_tag="ACHAT0_002895"
     CDS             275..>1731
                     /locus_tag="ACHAT0_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452776.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNR"
BASE COUNT          633 a          243 c          310 g          545 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac a
//
LOCUS       DAYYAR010000250         1716 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000250 DAYYAR010000000
VERSION     DAYYAR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.256"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1716
                     /locus_tag="ACHAT0_002896"
     CDS             <1..>1716
                     /locus_tag="ACHAT0_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8452777.1"
                     /translation="YDFSAQDIAMKFCIKRNVASHYLNQLFSDGKLLKNDSVRPVMFK
                     YNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLPLIIKGNSGVGKSFLASLIY
                     QYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKKGAFTGAEKDTAGMLSSANG
                     GYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEYANVRLILATTEDKNTSLLA
                     TFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITIDSKIFCSIELFTDLLSKTFE
                     GNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWIDPEISIDLKKMFLKSLSEI
                     DLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESDEFIALRNYVVNKISPIIDS
                     TGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFRMKNDKQKLLANEIWSSLEV
                     EESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITASAIARETNKLLNTYVYEAFD
                     MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVS
                     TALAIE"
BASE COUNT          617 a          221 c          315 g          563 t
ORIGIN      
        1 tacgattttt ctgctcaaga tattgcaatg aaattttgta ttaaaagaaa tgtcgcttcc
       61 cactatttga atcagttatt ttcagatgga aaattactaa agaatgattc tgttagacca
      121 gtaatgttca agtataatca acaaaagcca aaagactgct tttctaagtt tattggagca
      181 gacatatctt tgaaatccac tatagataaa tgcaaagcta ctgtaatgta tccccctaat
      241 ggcctcccat taataataaa aggaaacagt ggcgttggaa aaagtttttt agcaagtttg
      301 atctatcagt atgctcttga tagaaaagta attcataatg acgcaaaatt tgttgttgtg
      361 aattgtgcag attatgcaaa taatccagag ctattgtcag cagttttatt tggatataaa
      421 aaaggagcct ttactggggc tgaaaaagat actgcaggga tgctatcttc tgctaatgga
      481 ggataccttt ttttagatga ggttcacaat ctaagtgcag aaaaccaaga gaaattgttt
      541 cttcttatgg attctcaaaa gtatcgtaaa cttggtgaaa gtgtgcaatg ggagtatgca
      601 aatgttcgcc ttatcttagc cacaacagaa gataaaaata cctctttgtt agcaactttt
      661 agaagacgca tcccagctga aatcactctg ccagattaca ataatagaag tacatctgaa
      721 aaaattcaat tattatttga atttcttaaa gatgaagcaa ttacgattga ttcaaaaata
      781 ttttgttcga ttgaactttt tactgatctt ttatcgaaaa catttgaagg aaatgttgga
      841 gaattgagga atgaaatcaa gcttctttgt gcagaaggtt atttaggaaa taagagaaag
      901 tcttcaattt atttagggga caaactagac agcggttttt ggatagatcc agaaattagt
      961 atagatttaa aaaaaatgtt tcttaaatca ctttctgaaa ttgatttgat tgaacttttt
     1021 caaaatcaca tttcaattga agaaagcatg agtcaattaa gagatagaat acttaaagag
     1081 tgtgcaggtt cttctgagta taattattta gaaagtgacg aatttatagc attaagaaac
     1141 tatgttgtta ataaaatatc accaattatt gattcgacag gtctatgttt attagatgaa
     1201 ttcttggcgg atatcagtct atttataatg tttattgatg taattgaaga aacatcaaga
     1261 aattttttgc tgtctaagtt cagaaaattt cgtatgaaaa atgataaaca aaaattattg
     1321 gcaaatgaga tttggagtag tttagaagtc gaagaaagta aacaagaact tttgttgaaa
     1381 tggatacttt ttcttgtaaa gaagttctat gtgaaaatac cagaaaccca ttgtctgata
     1441 gttatgcacg gtaaaataac agcttctgcg atcgcaaggg aaacaaataa gttgctgaat
     1501 acatatgttt atgaggcttt cgatatgcca attgaaggtg agactagtga cttaattaac
     1561 ctaatcaaca atttttgtaa aagtatagat acgacgaatg gactcattct gctagttgat
     1621 atgggatcat tggaacaaat gtatgaaaaa attgaatcca atgtaatagg tgatttggta
     1681 attttaaaca atgtttctac agctttagcg attgaa
//
LOCUS       DAYYAR010000251         1656 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000251 DAYYAR010000000
VERSION     DAYYAR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.257"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHAT0_002897"
     CDS             64..972
                     /locus_tag="ACHAT0_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8452778.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1589
                     /locus_tag="ACHAT0_002898"
     CDS             972..1589
                     /locus_tag="ACHAT0_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452779.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          609 a          221 c          345 g          481 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt taatcagtca tatgtagatc
     1501 aaaaattcca agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt
     1561 ttacttttga aaaaattggg gaggtataga gaatgacaga tatcgtaaaa ataaaacaaa
     1621 gcaatgtaca ggtttatcca caaactcatt ggaatg
//
LOCUS       DAYYAR010000252         1643 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000252 DAYYAR010000000
VERSION     DAYYAR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.258"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..1596
                     /locus_tag="ACHAT0_002899"
     CDS             130..1596
                     /locus_tag="ACHAT0_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8452780.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          461 a          296 c          337 g          549 t
ORIGIN      
        1 atattgccat taaaaattac ttgcttttat agaaaattag tttatatttc ttaaataagt
       61 tttttagtta aatttttcgg taaagatgta taatattttc tgaaaattta aagaattggg
      121 gtatagatta tggaaaaaca gcaagacaag ggcttcttcg ggcagccgaa gggattgtca
      181 actttgttct tcactgagat gtgggaacga tttagttatt acggtatgcg tgcattactt
      241 atctattata tgtacgatac agttgccaac gggggacttg gattaccacg agcaacagca
      301 ttagctatca tgtcgatcta cggatctttg atctacatgt ctagtatcgt cggcggatgg
      361 gtatctgacc gagtgttagg agcaaaacgc actgtattct tcggtggagt tttcattatg
      421 cttggccaca tcgtcttggc tttaccattt ggtgtaagta cattgtttgt ttcgatggca
      481 ttgattatta tcggaaccgc ctttttgaaa ccaaatgttt ctggcatggt cggacatctt
      541 tattcaaaga ctgacttgcg aagagatgca ggattttcga ttttttacat gggtgtcaac
      601 ttaggggcat tattagcacc actaatcgtt ggtacaatcg gtcaagaata taactatcat
      661 ttagggttct ctttagctgc tattgggatg ttctttggat tattacaata catcatccaa
      721 ggacgcaaga cattagacgg agtgggtaca acaccgccga atcctttgac aactgaagaa
      781 cgtaaaggat tcatccgtaa cttgatcatc gcacttgttg ttatcgctgc agtattcggc
      841 ggggcacaac taactggtca tctgacaatt gacttttttg tcaatacaat cagtgtattg
      901 gggattatct taccgttgta ttactttatc aaaatgctga cttcaaaaga tgtaactgct
      961 gaagaaaaac caagagtatg ggcttatatc ccattattcc ttgcagcaat cattttctgg
     1021 tcattacaag aacaaggttc atctatctta gctttatttg ctagtgaacg tacacaatct
     1081 acgctatttg gtgtaacgat ccaagcaagc tggtatcaat cattgaatcc aattttcatc
     1141 tttattctga caccagtatt cgtaacgatg tggacgaaac ttggtgaacg tcaaccatct
     1201 acagtagtga aattctctct tggactttta tttgcaggat tatcgtactt gctattgatg
     1261 ttgccgggta tgctttatgg tacaagtgga aaagtaagtc cattgtggtt gttgggaagt
     1321 ttcttcatcg tggttattgc tgaattgtgt ctttcaccag ttggtttgtc tgtgacaaca
     1381 aaattagcac cacgagcatt tgaatcacaa acaatcgcta tttggttctt agctgatgct
     1441 tcttcacaag caatcaatgc acaaattgca cgcttctaca caccgggaac agaatctgct
     1501 tacttcggta ttatcggtgc agtagcaatc gtcggtggta tcttgctatt tactgtgaaa
     1561 gaaccaatca aaaaactaat gggctctatt cattaatcaa tgaacataaa aacaggaatt
     1621 gcttatttgc agcagttcct gtt
//
LOCUS       DAYYAR010000253         1640 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000253 DAYYAR010000000
VERSION     DAYYAR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.259"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..>1640
                     /gene="ltrA"
                     /locus_tag="ACHAT0_002900"
     CDS             416..>1640
                     /gene="ltrA"
                     /locus_tag="ACHAT0_002900"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8452781.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRL"
BASE COUNT          586 a          299 c          388 g          367 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag
//
LOCUS       DAYYAR010000254         1630 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000254 DAYYAR010000000
VERSION     DAYYAR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.260"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..240
                     /locus_tag="ACHAT0_002901"
     CDS             <1..240
                     /locus_tag="ACHAT0_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452782.1"
                     /translation="KKLLKQFAGIGNANVKFICLSCGEKESIPMDVVKFLDDADIAND
                     PNNPPQFTCEKCGKEMYPEYYRNALGIEFKISDVQ"
     gene            394..>1630
                     /locus_tag="ACHAT0_002902"
     CDS             394..>1630
                     /locus_tag="ACHAT0_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8452783.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNPHVHM
                     VVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQAAYKN
                     NGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKRTNTK
                     ETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKLFINL
                     NEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHAKRFM
                     REENRKIDQKEFRQQYEEEK"
BASE COUNT          584 a          248 c          365 g          433 t
ORIGIN      
        1 aaaaaacttt taaagcaatt cgctgggatt gggaacgcca acgtgaaatt tatatgtcta
       61 agttgtggtg aaaaagaatc aatcccaatg gatgtagtaa aatttttaga tgatgcagat
      121 attgcaaatg atccgaacaa tcctccccaa tttacctgtg aaaaatgtgg gaaggagatg
      181 tatccagaat attatagaaa cgcccttgga atagagttta agatcagtga tgttcaataa
      241 gaaggttaat catacaaaaa gtccaccgga gaattcattc tccggtggtt ttcttaaaca
      301 gtaaagaaag tataaaaagt taagggctaa aaaggctgca ctaccagtct tttagccctt
      361 ttcattttgt ttttgaacaa gtaaaatatt actatgaaag cgaatattct cgaagcaatc
      421 ttttttgacg aacaccataa ttgggataac tttgttgaga aatacgggcg aaagattcgg
      481 ctagtcgtca aacgggaagt aaacaagttt agatattgtg gagatattca aaaaggatac
      541 cgactgtttg tctgcgaggg atgtcatcaa acaaagtttg ttcccttgaa atgtcgtgga
      601 aagttctgtc caacgtgtgc aacaggtgaa agccaaaggt ggtcagcggt tgtggcgaat
      661 gatatgttta aagtgacaca tcggcacgta atctttacga tcgatgaggg gttacgggaa
      721 atctttatga tggattcctt actgaaagga ctaatggatg aagccgcaaa aattattctt
      781 gcctattttg agaaaagaaa ggtgaaggca ggtatagtag ctacaatcca cacctttgga
      841 tcaaaactag aatttaatcc gcatgttcat atggttgtta ctatgggtgg cttaacaaga
      901 gatggaagtt gggaagaata cgattattta cctttttcta tgttaagaaa atactggaaa
      961 aatgctgtat tgaaactaat tagacgaacg ttaggtgagt gggacaagca aagagttcaa
     1021 tcaagattac aagctgcgta caagaacaat ggagaagggt tttacgtaaa tgctcctaaa
     1081 aggagcagga caaatctaaa gtcactactc caatacatca gtcgttatat gaagcgtggc
     1141 ccgatcgcat taagccgaat cttaatgtat gatggcgaca ctgtcatgtt caactacatg
     1201 gacaaacgaa cgaatactaa agagacgatg accatgagta tagacgaatt catagctgca
     1261 ctgattcgcc atattcaaaa taagaatttc aaaacgattc gtcgctatgg aatctatagt
     1321 cgtagaatca aaacattaat gaaaaaggtc atgaaggagt atcagaaacg agtaaaaaag
     1381 ctgtttatca atctgaatga ggccttaaga ccaaaatcct gggaagaaag aataaccgaa
     1441 gaatttggtg aaaatccctt agaatgttct tgttgcggtg agtactatga attcgttggg
     1501 atgagtgtgc gcaaaaatgg acggttagtt attaaatatg cgaaagataa ccatgcaaag
     1561 cggtttatgc gagaggagaa tcgaaaaatt gaccaaaaag aattcaggca acagtatgaa
     1621 gaagaaaaag
//
LOCUS       DAYYAR010000255         1624 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000255 DAYYAR010000000
VERSION     DAYYAR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.261"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..427)
                     /locus_tag="ACHAT0_002903"
     CDS             complement(137..427)
                     /locus_tag="ACHAT0_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452784.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="ACHAT0_002904"
     CDS             complement(545..805)
                     /locus_tag="ACHAT0_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452785.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="ACHAT0_002905"
     CDS             complement(942..1355)
                     /locus_tag="ACHAT0_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8452786.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          611 a          263 c          237 g          513 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccga
//
LOCUS       DAYYAR010000256         1619 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000256 DAYYAR010000000
VERSION     DAYYAR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.262"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..276
                     /locus_tag="ACHAT0_002906"
     CDS             16..276
                     /locus_tag="ACHAT0_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452787.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(638..877)
                     /locus_tag="ACHAT0_002907"
     CDS             complement(638..877)
                     /locus_tag="ACHAT0_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452788.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1163..1477
                     /locus_tag="ACHAT0_002908"
     CDS             1163..1477
                     /locus_tag="ACHAT0_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452789.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          557 a          219 c          286 g          557 t
ORIGIN      
        1 aaggatggat gaaatatgaa taacgaaact atcattttag gaaaaaaata tatttgccaa
       61 cctattggct tgaaacaaaa agtgattgga gaagtaatca gtaaattaga aaattgtgtt
      121 gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga
      181 aaagtagtgg caaaatatac tcacgtttat gagttggaaa caacagaata tctatataac
      241 tatagcaaca aatatgaaca tactttagtt tcataaagat ttaaataaag tgccttcaaa
      301 agcgagaaga ccaagctttt gaaggcactt tatttagttg gcagtgattg ttcatgattt
      361 ttgatttatt caaaaggttc gcctagagaa tctgtctctg taacgaggcc tggtggagaa
      421 actcgataaa aacctatact gccagaactg tcctattttc ggatagacaa actagtagtt
      481 ttaaaaagat catccgatcc aatcatttca aaaaatgcaa aactgatatc aggatcattt
      541 agctcaggct tgttatcgta aagcatttgt aaaggttgca tttagttagg agtaaacgcg
      601 aaactatcat cattatttgc tgaagaagat gaagaagcta attcagagtt tgtttgaaat
      661 aaaggagacg tgctgacagt ttctttaccc tgttttttgc agaggtagaa acttttatat
      721 cggaagtttc taccggagaa tttttctcat ttgtagaaat tgttgaatcg atactattcg
      781 aaatttgcgt atctgaaggt atgttctttg atgcagaagt cccacaagca gtaagtaaca
      841 ataaatttga taaactgata aacagaattc ttttcattat gtacacactc cttatagtat
      901 cgtttagcat acacctatgc tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt
      961 tatagtatct attagtctta gtaatacata tatggagagg gtgatatccc agttcaaacc
     1021 agatacattg gttaccatct gtattaacga ttatgcctct aaacgctttt tttatgaaaa
     1081 aatttataag tgaaaacgta ggtatgatat tatctagagg taattttttt gggtatttaa
     1141 atagttgcta ggaggatagc ttttggattt ttacagtaat tttatcttaa taatagcaat
     1201 attattactt ctaaatattt ggttttttga taagagtaga aatgcaggga taggttttag
     1261 aaccaaacga agtacgtctt ctgaaaaaaa gtgggtatat tcacagacta ttttttacgg
     1321 aggggttatt tcaatcagtt tactttcttc aacactttat tcttttaatg ttatagacgt
     1381 ttccatgtca aattttatat ctattattgg aatccttatt tctgcaatta ttacacaact
     1441 acttcttgtg tttgaagaga agtcaaaaaa taattaaagc aattatgcct tttagtacag
     1501 gaattattac ttcttacaaa tgtatagcga atgtgaagcc tgctgtttta attgttctag
     1561 aaaaactttt gagtgaacaa ttattttatg ttaggatgaa ttaatttctc tagagttta
//
LOCUS       DAYYAR010000257         1616 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000257 DAYYAR010000000
VERSION     DAYYAR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.263"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..240)
                     /locus_tag="ACHAT0_002909"
     CDS             complement(<1..240)
                     /locus_tag="ACHAT0_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8452790.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFET"
     gene            complement(258..578)
                     /locus_tag="ACHAT0_002910"
     CDS             complement(258..578)
                     /locus_tag="ACHAT0_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8452791.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            877..>1616
                     /locus_tag="ACHAT0_002911"
     CDS             877..>1616
                     /locus_tag="ACHAT0_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8452792.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEG"
BASE COUNT          600 a          271 c          260 g          485 t
ORIGIN      
        1 tgtttcaaat tttgagtgaa aagttgtaga agtatcgtac aaaactgaaa attcaaaatg
       61 agaggcgctg aaatgtgcaa gttcatgaga taaatgaaag gcttctgcag tttcaccgta
      121 tagattttca ttcaaaaaaa tgattctggg ttttggatag tagaaaccag gctctttcat
      181 ctccatataa actactttca aattgtattc gctcaacatt tctttcaatt tcaaatacat
      241 acaaaccatc actccaacta ttcattttcc tctaaagctt tagcaattgc aatcgcttta
      301 cgcattgtct ccttagatat ttcttttcca tcaaaagaaa aaacagtatc gtcttctgat
      361 aaatccacat gtttaggggt ttctctttct tctctaccta gaaggtagtc tacagagaca
      421 tcgaaatagt cagcaatccg acttaattca tctgaattag gagtattatt tttccacttt
      481 gctagataac cgttagaata tccaaaatta atttctaact gccgtataga tatctttttc
      541 ctcttcgcca agtcttttat tatttcatag gtattcatcg ataaaacaac ctttctaaat
      601 gtttacaaaa aaagtttaga aaaatacgca gaaataattt gactaattct gagtaatgag
      661 ctatactata tcttgtaaac agatttaaca actaaaaaga caacaaaaaa cactattgat
      721 atgtaaatgc agaccgccaa gaaagcttta aaatcaatgt ttttatgtct tatttaatta
      781 tgctcttagt atagagtatt actcagatac tgtcaattga atttagaaaa aagttgttaa
      841 atttgtttac gaatataaaa gaaaggaatg agaaatatga acacaccgca aatttttaat
      901 ttcgaacaaa acgaagttcg gacaatttta gtaaatgatg aaccatattt tgtaggcaaa
      961 gacgttgcga gtgttttggg ttattcaaac actaaagatg ctttgtcgcg gcatgtagat
     1021 ttggaagata agatggggtc gcgaatcacg acctcaggtc aatcaagaga gatgacaatc
     1081 atcaacgaat ctggtttgta cagtttaatc ttaaaatcaa aacttccctc tgccaaaaaa
     1141 tttaaacgtt gggtaacaag tgaagtgttg ccagcaatta gaaaacatgg aggttatcta
     1201 actccagaaa aagtagaaga agctttgctt aatccagata caatcattca attagcaact
     1261 caactaaaag aagaaagaac tggaagatta atcgcagaac aaaagattgc agagtacgaa
     1321 cctaaaatct cctatttaga tagcatatta tcttctacag attcagtaac aattagtcag
     1381 attgcagcag attatgggat gtctccacaa cagatgaata aattacttca taaactaggt
     1441 attcagaaaa aagtcggtaa ccaatggtta ttgtgcaaaa aacacatgaa ccaaggatac
     1501 acaaaatctc atacaactga gatcccgaaa gccgatggtg gcactaaaat tgtaatgaat
     1561 accaaatgga cacagaaagg gcgtctattt atctacgaat tactaaaaaa agaagg
//
LOCUS       DAYYAR010000258         1569 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000258 DAYYAR010000000
VERSION     DAYYAR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.264"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..851
                     /locus_tag="ACHAT0_002912"
     CDS             90..851
                     /locus_tag="ACHAT0_002912"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8452793.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(966..1406)
                     /gene="fabZ"
                     /locus_tag="ACHAT0_002913"
     CDS             complement(966..1406)
                     /gene="fabZ"
                     /locus_tag="ACHAT0_002913"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8452794.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          535 a          288 c          278 g          468 t
ORIGIN      
        1 atgataaatt ccaataattt aagtaggtat tttccctcta ttttgttatc attaaagata
       61 acaaaatagt aaattggaaa ggaacagtta tgaagaaaaa taaagttatt ttaagtatta
      121 gtttagcagc agcattgctg tttgctgctg gttgttctgc aaacaacacg acttcttcta
      181 gttcgtctac agaaagttca aaaagtacat ctactgcgga aagcacgact gccagtagcg
      241 tcgatttgaa cgcattggaa ttaccacaat tgacgaaaga tgtcgccgaa aatgaagatc
      301 tggtcgaaat ggtgacgaat aaaggcacaa tcaaaatcaa attatttccc gaacacgcac
      361 caaaagcggt tgaaaacttt atgacacatg caaaagacgg ttactacaac ggactgactt
      421 tccatcgcgt catcaaagat ttcatgatcc aaggtggcga tcctaaaggc gatggcactg
      481 gaggggaaag catttgggga gaaggatttg aaacagagat caacaaccac ctttataata
      541 taagaggtgc gttatctatg gctcgctcac aagatcctaa cagtaacggc agccagttct
      601 ttatcgttca aaatacagat gatatgcatg atggtctttt aaaagacgac tatcctcaag
      661 caatcatcga cgcttataaa aacggcggtt atccttcact agatggccat tatactgtct
      721 tcggccaagt gattgaagga atggatgtcg tggatgctat tgcaaatatc gaaacagata
      781 gttcagacaa accaaaagaa gaagtaaaaa tcgataaaat caatatactg caagaagcaa
      841 aacaaaaata aaaaaataac gtctgaatcc tgcatagatg acaacgtatg ctaggattca
      901 ggcgtcttct ttttacataa aaagcaagta agcttccttt tgttttgcta tatcaggttt
      961 tatagtcaag cattttggtt gacgatgaac gtgaactcgc actcacagac ttttttatca
     1021 tctacatatg ctgcagataa cgctgtaccg actgatccct tcattttggt gatttcaaaa
     1081 tgacatttca gcacatctcc tggaacaact ttttgacgga atttggcctt attgatccca
     1141 ccgatataag ctctttttcc tagaaactct tctgatttta ggataagtat cgaacctgct
     1201 tgtgccagtg tttctagaat caacgcaccc ggcatcactg gattgtcagg aaagtgacct
     1261 tggaaaaaat cttcattgat tgttacattt tttgtcgcaa cgatcttttt tcctggttct
     1321 aaagtatcta cataatcaat ataacaaata ggataacgat taggaatcaa ttccattact
     1381 tcagtagcag ataatacgta agacataatt aaactccttc tttcaggcaa ttgccatttt
     1441 cattactttg actatcaaag tatcatattc ctaaatattt agcaatcttt ttttaattta
     1501 agaaaaaata gtttgacaat caaaacattt tgactaataa ttaagaaatc aagaagttat
     1561 tttagctat
//
LOCUS       DAYYAR010000259         1556 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000259 DAYYAR010000000
VERSION     DAYYAR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.265"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..839)
                     /locus_tag="ACHAT0_002914"
     CDS             complement(<1..839)
                     /locus_tag="ACHAT0_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM8452795.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSR"
BASE COUNT          500 a          311 c          266 g          479 t
ORIGIN      
        1 attcttgagc tggctgcagc tagtgagtgt ctttcattta tccataagat aaagcttgga
       61 ttcaataaat aaataaatgt tttggttaaa gctgtgacca gcaaatgagg aaccgtgcga
      121 gccagttgat ctgccaatgc aaacatttct ttaggtgaag gcgctggcgt gtctgccaac
      181 ggatactcca gtcccaatgc ttggtaaccg tgcataccat agcagtggta cggtagtaat
      241 tctaacggta cgaaccgatc aagatctgcc acaaaacaag caatcgtcag cagctcggtc
      301 aggttcatat tgatacctgg aaccacagga actcgaattc gcacttcgca gttaggatac
      361 tcaattaaaa agcgacgaat attcttcaaa atttgttggt tatctaagcc ggtccattgt
      421 ttgtggagct tgctggtaaa catttttaca tccacaaaca atgtatccac atagggagct
      481 accttttgga tttcattata agcaccacaa aagctggttt ccagtacggt attgattcca
      541 atgtatttcg atttttgcaa aatttcttta gcaaaatccg cctgaaccag tgcctcgcct
      601 ccgctaatgg tcactccgcc atccgagtga aagtaaaaaa cagcatcctt ctcaatctca
      661 tccattacct cttcagcggt catggtctgg ccaaatcctt tttcaatctg cttgctttgt
      721 gattcgggaa tcgaacacca ggcacagcgt aatgggcagc cctttaaata aacaacggtc
      781 cgtaaaccag caccatcatg tagagaaccc tgttctattc ttaatactgt tccttccata
      841 tagatttttg aattacacac tagtttaaac taaaacacac aaggaaaaac ttaccattta
      901 atcataaaat tagctcatat aaatatacta ggatgtaaaa tagactggtc aaaatctggt
      961 caaaaaagag gactaataca ggtagtcctc ttttttgtta ttttaatatt ctaaatcatt
     1021 ttttattggt ttattccctt attcgatgta gactcaaatc actatatcac tagatatcaa
     1081 acggcgtcat acggaatcaa aaacatcaaa tttgacctgt taaatggtca ttgaataaca
     1141 ttcaaaatca ataaaacgga gtcagacacg gagtcaaaaa cgctgagagt aaaaaatata
     1201 tacagccttg taggtaaagt accacagggc tgtatatttt ttatttcaga ttaccttaaa
     1261 tgattccatt tactttttat ctcatttttt cctactagtt agtatcaaat actctcctat
     1321 tttctcaata aactattttt ggatacatgg tatctgaaac agctaaaacc ttgtcattac
     1381 agcaaaaaat cacgttatcc agactgtaga caaagtccat tttatggatg atgtctacag
     1441 tcttttcttt tataatagat ttatgaaaac gaatgcaagg agtgatagct atgatgtcta
     1501 aaaacaatac gaatggtaga aatcaatttg cgatgcttac gattgatgat ctagtt
//
LOCUS       DAYYAR010000260         1517 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000260 DAYYAR010000000
VERSION     DAYYAR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.266"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..437)
                     /locus_tag="ACHAT0_002915"
     CDS             complement(138..437)
                     /locus_tag="ACHAT0_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8452796.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            788..1420
                     /locus_tag="ACHAT0_002916"
     CDS             788..1420
                     /locus_tag="ACHAT0_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8452797.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          524 a          242 c          207 g          544 t
ORIGIN      
        1 ggataaaata caatataaag aaacatctgt ttctttattt agaataaaaa aatacagact
       61 ctgttcaaat aaatgaacct agcctgcatt atgagattga ctatactttt taattcattg
      121 agatgaataa ctcgttttta taaatgccca aaagtaatcg gtgccgaagc cattcctcga
      181 cctaattctt tttcaaattt tcctattgtt gatatctcca tataaaacgg tgatactggt
      241 ttagacaatt ctcccctgat tgctaacgga cgtaaactac gttccaaatt atttatgact
      301 ttaggataat actgaccttt ttctaagcct gtctttgcgt ctatcaaaag tatacgttcc
      361 ttttctttga tctctggatt taaaacgaga tcatataatt ttagtaccat gttttctatt
      421 tcttcacttt tagacatcac gatttcctcc caaaaatata tttcttccca ctacattcta
      481 taaacaaagt atacgctaca tctagatttt tttaaatcat tactggattg ttaaaacctt
      541 tcaatacact gttatggcaa cgatttttat tttaaaaaca ttctaaatta aatcagtaaa
      601 aatttttatt ctaagatttt tattagaatt acgcctaatt tgtgtctctc agggcaattc
      661 taagaacgtt tcaagagata gcgacactat cgttatcgaa aaaggcgaaa acctatctca
      721 ttttctgcta atgtaagttg gtttagcttt tgttaatatg actttatgaa aatggaggta
      781 attaaatatg atttttccta aagttaatga agcaagcttg aataaaggat ccttttttca
      841 tatctatgaa aacgaagatt ttgaactgta taatatcctt tcacaaaaag aggtcatcga
      901 aaaccaaata atctgccatc caattattta caaatctatt ttcaagtatg cattttttgc
      961 ttccaatgat tttcaaagaa tggagattac cgactgtata ttcgaaaact gtgatttttc
     1021 aggctgtata ttgacaaaaa gcatgttgca tcgcgtgaaa tttgtcaatt gtaaattaac
     1081 aggtactaaa ttgatggaaa gttatattgg gaacacgtta ttcgagaatt gtaaaatgga
     1141 ctatgtaaat ttatctggtt caaacataaa agaaagtacc tttgagcact ctgtcttatc
     1201 aagcgcagat tttgtagact gttccctcac taaaataatg ttttctacta atgatttaga
     1261 aaatgctagc ttttttgata ctgatttaaa aaatatcgat ttaagtagca actcctttga
     1321 aatgattgaa gtacgctcgg aacatctaaa aaattgtacg cttaatcagt tgcaagcact
     1381 tggttttgct agaaaatttt tacaaataaa agtagtataa tcaaaatgaa tatataagat
     1441 aaatttttag caatacaaat atccataaaa aaacattgcc tattttaact cttcatgtat
     1501 actgactcta tcttcaa
//
LOCUS       DAYYAR010000261         1515 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000261 DAYYAR010000000
VERSION     DAYYAR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.267"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            264..947
                     /locus_tag="ACHAT0_002917"
     CDS             264..947
                     /locus_tag="ACHAT0_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8452798.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(984..1481)
                     /locus_tag="ACHAT0_002918"
     CDS             complement(984..1481)
                     /locus_tag="ACHAT0_002918"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8452799.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          482 a          255 c          249 g          529 t
ORIGIN      
        1 ttaaagaaga ttgggcttca tggatataat atatatttgt aaataaagtt aattcagtta
       61 taattatcaa aactacacaa ttattaaagt ttttcatatc tctttgttta gtaagttttt
      121 ctaaatctta acccgtggtg ctagttaatt ttttcctaga ggaaaatgtc taaacatgtt
      181 ggtaaatcaa caaaaaaacg catatagtag tctcaaaact cccaagaaat gaggactatt
      241 taacaaacta cgatggttac gtcgtgacaa ctgaacattt tgacttagcc aatcctgtta
      301 ctaaagtcga tgatatcccc gattatgaaa tgtacagtca aacaatcgat agcttaaata
      361 agcgttttgg caatcgtgtt ttaaaaggca tcaaaatcgg ttacatcgct tctgaaaagg
      421 atcggatcat cgactattta gccgataaag attacgattt aaaattactc agtgtgcatc
      481 ataatgggca atttgattat ttagatgacg aagtcaaaga tatggatcct gctattgtga
      541 ttcctcagta ttttgcacaa ctttcagaag cactagttgt tatagaagca gatgtctttg
      601 ctcattttga ctatggtttt cgggtatttg gtctatccgt tgctgaattt aaacaatatg
      661 aagcgcagtt tttaccgatt ctagatcaag taatcaaaaa taaattggca ttcgaattaa
      721 atgctaagag cgcttacttg tatgacaatt tagcgttata tgaatatgtc atcgatttat
      781 atctttcacg tggtggtacc ttgtttagtg ttggatcaga tgggcattac cttgaacatt
      841 tccgtttcca tttcgatgat ctttttgctc tgttaaaagc aaatggtgtg acggaattag
      901 cgatttatca aaaaggaaag cgaattatgg ttccattacc agtctaaaaa aacgtcctca
      961 gcatcaaaag tgaggacgtt tttttagatt tctttttcta aaatcggaca aatacgatct
     1021 tgttttccta aaggaaaatg tttgatcccg atttttttaa atccatgacg ttcataaaaa
     1081 cgaatacctg catgattttt ttcataaaca gtcaaacgta aataacgttt ttgtttcgtt
     1141 ttagcataac gattcgcttc atcgagtaac cgttgcccta accctttgtt ttgataatct
     1201 ttgagcagat agagccgttg aatctctata gcatcttttt catcattgac tttcatataa
     1261 ccaatgagta tttgatgatc cttaatacca ataaatttac tggttgactg tgccaattct
     1321 actgccaata tttcagttgt caattgtgtc tgtaaatatc cttgcatatc aagtggatct
     1381 gcgccatcga taaacgattc ataaaaactg acaacactta caacttgtaa agcatctaga
     1441 tcatcataat ctaaatacgc tagtgtgatt tgattgttca tctaactcct ccttttgaca
     1501 tagtatacta taaat
//
LOCUS       DAYYAR010000262         1486 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000262 DAYYAR010000000
VERSION     DAYYAR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.268"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..1412
                     /gene="nhaC"
                     /locus_tag="ACHAT0_002919"
     CDS             45..1412
                     /gene="nhaC"
                     /locus_tag="ACHAT0_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8452800.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT          388 a          283 c          327 g          488 t
ORIGIN      
        1 ttttttcatg gcagggcctt ttcatttgaa ataggagatc gacgatgaat aaaaatattt
       61 ctttaaaaga aagtatcgct gtattgatcg tgttgttggc actcctagga gtactgatca
      121 tcggattcca gctctcgcca catatcccga tccttgcagc tttcatgtgt ttgttgtttt
      181 atggcagatt taaaaagttc acatgggatg agatccatga cgggatcatc aaaggaatca
      241 ctccagggat cattccaatc ctgatttttc ttttgattgg tgtcttagtt gcttcatgga
      301 ttttatctgg aaccatccct acgattatgg tttatggatt taaacttgtt tctgtgcgtt
      361 tctttttacc gacagcattt cttgtttgtg caattgtcgg agtgactgtc ggcagttcat
      421 ttacaacgat ttcgacgatt ggaattgctt ttttggggat cggtcattta cttggattca
      481 atgatgcaat gactacaggc gcagtcgttt ccggtgcctt tttagggaat aactgctcgc
      541 ctttatcaga tacgaccaac ttggcagcgg gtattggagg agttaatcta ttcgaacata
      601 tcgctggtat gcgttatacg gatttcccag cattcgtcct ttcgttgatt ttttatacga
      661 tattaggaca taacagtcat tcagccgatc tagctagtat caatgaaatg atccaaaaca
      721 tgaaagcgag cttttggatc tctccttgga cattgattcc actagttatt ttatttgggg
      781 gggcaatcaa aaaaattccg gctatcccta cattactagc gggatcgaca gctgctctcg
      841 ttctttcttt tatccatgag aaaagtttga cggtcggaaa agcagcagac atcttgatga
      901 atggttatgt cgcaaataca cctgatccaa agctagatga actgctttct cgaggcggga
      961 tcatgagtat gttaggttct gctagtttga ttttacttgc attggcatta ggtgggttac
     1021 tgatcaaata tctgatcgtt gataccatcg ttcaagaatt aaaagaaaag atggaccgac
     1081 cttctcgttt agtcgggttt actgccttga gctgtatcgg aatcaatctg atcgttggag
     1141 aacagtatct gtccatcatt cttccaggag aaacattcaa gcgttctttt gatgacgttg
     1201 gcttatcaaa aacctatttg acacgaacac tagcggattc aggtgcgacc gtcaattctc
     1261 tcgtaccgtg gggagtcagc ggcacattca ttatgggaac gatgaaagtt agcgcgttgc
     1321 attacttgcc attcgccttt ttctcgattc ttgcacctgt ctttacaata ttaggcggtt
     1381 ttttattgaa tcgtcgacgt gtaaaaaatt gaaggaagga aaaaagaggc tgggacagaa
     1441 gcgtttaact ccaagaaata agaagaaact caagaaaatt gctttt
//
LOCUS       DAYYAR010000263         1476 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000263 DAYYAR010000000
VERSION     DAYYAR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.269"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..503
                     /locus_tag="ACHAT0_002920"
     CDS             75..503
                     /locus_tag="ACHAT0_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8452801.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACHAT0_002921"
     CDS             490..732
                     /locus_tag="ACHAT0_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452802.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACHAT0_002922"
     CDS             complement(1032..1247)
                     /locus_tag="ACHAT0_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452803.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..>1476
                     /locus_tag="ACHAT0_002923"
     CDS             1354..>1476
                     /locus_tag="ACHAT0_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8452804.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVL"
BASE COUNT          466 a          224 c          294 g          492 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtatta
//
LOCUS       DAYYAR010000264         1422 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000264 DAYYAR010000000
VERSION     DAYYAR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.270"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACHAT0_002924"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACHAT0_002924"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8452805.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1131..1406
                     /locus_tag="ACHAT0_002925"
     CDS             1131..1406
                     /locus_tag="ACHAT0_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452806.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT          439 a          284 c          213 g          486 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1081 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1141 aggataaaaa agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata
     1201 cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag
     1261 gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaaa
     1321 aaaaagaatt tcataaacag atgaaaaaac tgattgaaaa agaatttcca agaagtcata
     1381 aactctatgt gaatcagcaa ttttaacttc taactgttct tt
//
LOCUS       DAYYAR010000265         1392 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000265 DAYYAR010000000
VERSION     DAYYAR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.271"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1192
                     /locus_tag="ACHAT0_002926"
     CDS             <1..1192
                     /locus_tag="ACHAT0_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8452807.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
BASE COUNT          553 a          202 c          298 g          339 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct gatggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      421 agtaacgtat ccagatggta cgaaagatac agtgaaagtt ccagtagaag tgacagacaa
      481 tcgttccgat gccgataaat acaatcctga aggacaaaaa gtgactacag acttaaataa
      541 agagccagac gcatctgagg gaattaagaa caaagaagat ctaccaaaag gaactactta
      601 tacttggaaa gagaaagtag atgttagtac agctggaaac aaaaaaggga ttgtagtagt
      661 aacatatcca gatggatcta aagaagaagt agaggttact atttctgtag aagataaaaa
      721 agcaccgaat aaacctcaag ttgatcctat tacagagggt gaccaaattg ttactggtaa
      781 aactgaacca aatgcagagg tgacagttac attaccaaac gggagtcaat accatgggac
      841 agctgataaa aatggtaatt ttacagttaa agttcctaaa ttagaggcag gaacaaaagt
      901 gatagtaact gcaactgatg aatctggtaa tactagtgaa cctactaatg tagttgtttc
      961 ttcaaacgaa aaagacagtg aaaaagccgt tagtaaagat aataagactg acaatcaagg
     1021 tagcaagcaa aacaaaaata gaggcaaaag cagtcctcaa aagcagtcct caaaagctta
     1081 tcctaagacg ggagagattg atagtaatat ctttactatt agtggtggtt taatcctgtt
     1141 aggaacttta gggttattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1201 tttcttaaat cttctctgaa aagaggaaga atctttttct tcctcttttt gttttttatt
     1261 ttgggagata ggttgttagt taaaaatgaa gatacttttc atagaaggga aaagaatgtg
     1321 attaagtgaa aaaaatagta attattggat caaaccattc ggggctagct gcagctcgat
     1381 ttctaaactt at
//
LOCUS       DAYYAR010000266         1365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000266 DAYYAR010000000
VERSION     DAYYAR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.272"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..566)
                     /locus_tag="ACHAT0_002927"
     CDS             complement(<1..566)
                     /locus_tag="ACHAT0_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8452808.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMS"
     gene            complement(563..1216)
                     /locus_tag="ACHAT0_002928"
     CDS             complement(563..1216)
                     /locus_tag="ACHAT0_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8452809.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          442 a          218 c          119 g          586 t
ORIGIN      
        1 aaactcattt ttttatcaag tttcatcatt ttatcagtaa caatttttat ttcttgtata
       61 ttatttttta ttgattttgc ataaattaaa gaaataatag cagaaaataa aattgatatt
      121 aataaagaat atggtaaaaa tttaaggctt aaatctttcg catctttttg catatcagct
      181 gtagaaacaa attgtagatt tgttttttta ccatcattaa gctttatttc tctttcttca
      241 attatcaaag aattactatc actttctaaa ttaacattta tattatcttt gatttgtatt
      301 tcattcttat tatttttttc ttttatgaat acttttattt cactactctt ggaataaaac
      361 tccaaagttt gttctatata ttttatttct tttccattca tattactaga aatttcattt
      421 gccatattat gaatcttttc ttttctcgtc tccaaatatg ttttaggaaa gataaaaaaa
      481 attaatgaat gaactaatat aattattatt ccaagaacag aaaatatttg tatgaacatt
      541 tttggaaata tctttaattt tttcattttc tctccaattt atatcctaca tttcttatag
      601 tagttataca gtctaattgt aatttttttc taagttcttt tatatataca tctattactc
      661 gatcataagg agtttcttcg ctatctttcc atacataatc aataatttgc attcttgtta
      721 acacttgtcc attattatcc aataaacatt ttaatacttc tagttctttt gcatttacat
      781 ctatttcttc accatttatt ttagctgtat agctattaaa attaaccgat agatccttat
      841 attcaaactt ctctaaatgt ccataattct tcttaattaa agaatctatt ctagctttta
      901 agaccggcaa tgaaaatggc ttttctacat atccgtctac taaattagta aatgcatcaa
      961 ttttatattc ttcatcacta aatgcagtca agattagaat tggtaaattg cttttctttc
     1021 taatttcttt caacacttct aaaccattta taaaaggtat ctgaatgtct aagataacca
     1081 aatttatatc gctattaaat tttgacaagg cttctcttcc atctttagct tgaataacag
     1141 tatatccaaa ttctgaaaga tattcactta ttccctctct tatcatatta tcatcttcaa
     1201 cagttaatat tttcatatat acctccactt aagcctatac actagatatt atacagcata
     1261 ttatggctct ataatatcta gtgttggtga caccttctgt tgccaatact ttttcttttt
     1321 tctaataaat gcaaacagac accttgaaat cctttagaat aaagg
//
LOCUS       DAYYAR010000267         1254 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000267 DAYYAR010000000
VERSION     DAYYAR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.273"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACHAT0_002929"
     CDS             290..1135
                     /locus_tag="ACHAT0_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8452810.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT          475 a          149 c          208 g          422 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggat
//
LOCUS       DAYYAR010000268         1252 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000268 DAYYAR010000000
VERSION     DAYYAR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.274"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..710)
                     /locus_tag="ACHAT0_002930"
     CDS             complement(42..710)
                     /locus_tag="ACHAT0_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8452811.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            844..1131
                     /locus_tag="ACHAT0_002931"
     CDS             844..1131
                     /locus_tag="ACHAT0_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452812.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          474 a          210 c          211 g          357 t
ORIGIN      
        1 tgatatttct gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca
       61 agaggatcaa gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca
      121 gatccaattt ccagatactt cactttattc aatgtataag atagaataga acagctaagt
      181 aatgtataca gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa
      241 gtaatcaaga aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa
      301 atgatatctg ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca
      361 gaaagaatac ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga
      421 aggctctccc ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga
      481 aaagcgtgtt ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt
      541 gaagtgacag aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc
      601 ccaccatctg ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct
      661 gcgcctaaaa tgattccgca caattctgta aataaattta attttttcat ttctagcctc
      721 ttttcttttg aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag
      781 aggaaaatga aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga
      841 aaaatgcgag cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat
      901 caaggagttt ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat
      961 aaagcaaatc aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac
     1021 atctttcttt ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa
     1081 ctcattcctc tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata
     1141 agaaaacaag acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg
     1201 tcatgataca gtataatatt cgttgaacag gccaataaag gtgttctccc cc
//
LOCUS       DAYYAR010000269         1222 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000269 DAYYAR010000000
VERSION     DAYYAR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.275"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(377..1081)
                     /locus_tag="ACHAT0_002932"
     CDS             complement(377..1081)
                     /locus_tag="ACHAT0_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452813.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          363 a          212 c          217 g          430 t
ORIGIN      
        1 gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaaagtgtt
       61 agtagaaaat cgctttcgat tttccaccaa cacaaaatat tatagaaccg cttttttctc
      121 tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa
      181 tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata
      241 ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt
      301 tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt
      361 atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag
      421 gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat
      481 tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa
      541 taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg
      601 aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt
      661 ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg
      721 tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca
      781 cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa
      841 ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat
      901 ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt
      961 gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc
     1021 ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca
     1081 tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag
     1141 gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc
     1201 cgttttttga aagaaaaata aa
//
LOCUS       DAYYAR010000270         1217 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000270 DAYYAR010000000
VERSION     DAYYAR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.276"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..1198
                     /locus_tag="ACHAT0_002933"
     CDS             254..1198
                     /locus_tag="ACHAT0_002933"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8452814.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          409 a          182 c          256 g          370 t
ORIGIN      
        1 aggtgcgatt gctgtcttat tatatcgttg cagcgaattt ttttggattt attttcagtt
       61 ctaaactttt ggttgtatta tcaggggata taggtgtaaa catttgtcat ttatctgtta
      121 tgcgtattca ggaattcgtt tccatcaaaa attataataa caactattat aattaacatg
      181 ttgataaaat gaataaacaa gactatactt ttcatgaaag taacaaatag ttatgaaaag
      241 gagctgttta ttcatgaaaa aaacaagtcg taaagtagta atcgtcggta caggttttgt
      301 tggaacaagt atcgcttatg caatgatcaa ccaaggaatc tctaatgaat tagtattgat
      361 cgatgtgaat caagaaaaag cagaagggga agcattggac cttttagatg gtatggcttg
      421 gggcgatgaa aatgtggctg tatggtcagg tggctatgaa gaatgtaaag atgctgatat
      481 cgttgttatc acagctggta tcaaccaaaa acctggtcaa tctcgtttag atttagttaa
      541 aacaaatgcg tcaatcatgc gccaaatcgt aaaagaaatc atgggatcag gattcgacgg
      601 tattatcgtt gttgcttcaa acccagtaga tattttgaca tatatcgctt ggaacgaatc
      661 tggtctgcca acatcacgtg ttatcggtac tggaacaaca ttggatacaa ctcgtttccg
      721 taaagaaatc gcattgaaat taaaagttga cccacgcagc gtccacggct atatcttagg
      781 tgaacatggt gattcagaag ttgctgcatg gtcacataca actgtcggtg gtaaaccagt
      841 gtttgaaatt gtagaaaaag accatcgtat tgcccaagat gaattagatg tgattgctga
      901 taaagtccgt aatgctgctt atgagatcat tgatcgtaaa aaagcaactt actatggtat
      961 tggtatgagt actgcacgta tcgtaaaagc tatcttgaat aacgaacaag ctgtattgcc
     1021 tgtatctgct tatttaactg gtgaatacga cgaaaaagat atctttacag gtgtgccatc
     1081 aatcgttgac gaaaatggtg ttcgtgaagt tgttgaatta tcaatcaacg aagaagaaaa
     1141 agcaatgttc agcaaatcaa caagtgcttt aagagaagta ttgaacactg tattataaga
     1201 aacaatgaat atgaact
//
LOCUS       DAYYAR010000271         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000271 DAYYAR010000000
VERSION     DAYYAR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.277"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACHAT0_002934"
     CDS             414..947
                     /locus_tag="ACHAT0_002934"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8452815.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAYYAR010000272         1195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000272 DAYYAR010000000
VERSION     DAYYAR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.278"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHAT0_002935"
     CDS             <1..1076
                     /locus_tag="ACHAT0_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8452816.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAYYAR010000273         1183 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000273 DAYYAR010000000
VERSION     DAYYAR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.279"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..1128)
                     /locus_tag="ACHAT0_002936"
     CDS             complement(34..1128)
                     /locus_tag="ACHAT0_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8452817.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          334 a          256 c          189 g          404 t
ORIGIN      
        1 ttctagcgga ttatttctgc aataactaat tatctatttt ttattttcaa ttaatttaaa
       61 gcttgaccat ggtttcaaaa agtcaaggag gaataattcg tcatcggaga ttcttcctac
      121 aacattgaca cgtccgtcgt ttttcatatc cttcaaggcg atttgtgttt ctcctttgta
      181 ttgaccgtac ccttcattgt caatcagtac atccccatga tgcatatctc gcgtattatg
      241 aggcgggaaa tctttatctt tgtagtatac acgagtcatc gttgaacgaa ggatatattc
      301 tgagcgatcc cctcgataac tatgaaggtt atcaaacaag cagattcgtt cgttttctgt
      361 aataccttct tccgtatcta ctttcaatgt tggataatcc gcgttaaatg cttctgccat
      421 ttctttcaat tcttcttcac ttgcgtacgc atttccaata gaaatatcat caatcaatcc
      481 tgtaaggatt aaatgcttca cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt
      541 gcacaagcca tcttgcgtag gccaagggcc aaactcagca gattgcgaat tgacgaatgc
      601 catcgtattc aaattatatt ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc
      661 taaaccagag tatcgatgag gataaaaatt atgagaacct aataaattat ccgtgtttgg
      721 cgaataactc atgatgttgt caacgtaatt cgtgcccgaa ctcatattga tctcgatttt
      781 gataccaaaa ggattgcgcg tcatttttgc ttcttctgct cctgtgaatc cgatatctaa
      841 gcggaccccg taggcaccca tcttatggaa aaaagataag tcatcgtaag aaatctccaa
      901 ctgttcaaat aacgctggat tgatatcaac cattacttcc atgcctaggc tattcgcgta
      961 atcaactact tctttaaatt ctgaaagaac tttttcttta tcatcattga tctgcagcaa
     1021 actggtaaaa acccgtttat acccatactt atgagctaaa tccaagtacg ctttgtcctt
     1081 ttcaaatgtt gaccgttctg gataaatcga gattcctaat tttcccatat taccatttcc
     1141 tcctgcatct tttcgttttt gtatccactt acattatagc aaa
//
LOCUS       DAYYAR010000274         1146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000274 DAYYAR010000000
VERSION     DAYYAR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.280"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..577
                     /locus_tag="ACHAT0_002937"
     CDS             35..577
                     /locus_tag="ACHAT0_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452818.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            733..>1146
                     /locus_tag="ACHAT0_002938"
     CDS             733..>1146
                     /locus_tag="ACHAT0_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8452819.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMA"
BASE COUNT          475 a          121 c          231 g          319 t
ORIGIN      
        1 atatttcatc aacgtttcat aaaaggtgtg tagaatgtta ttgaaagtag ctataaaatg
       61 ctattgtgta aaaaataaag gaggaataat ttatgaaaaa atagtaccaa gaggagcatg
      121 cctattactt gcattatgtt tgagcttaag cgcagttggg tgtcaaaaaa gtaatgaaaa
      181 caataaaata aaagaaggac aatccataag ttctgaagag gaaggcatgt caattaatgc
      241 aaatggcaaa aatgaaactt ttaagccatc agattataca ttggaggcaa agaaagaata
      301 tgtttatgaa tatttagggc ttaaatttaa actctcagac aaatttagaa attatatagc
      361 tgataaaaag atagcgatgt tagatgatca aagtcctata gataaagaac taaagtatgc
      421 tattttgaca tttgagaaaa tgacagaaga acaaaagaat gcagttatag aaaagatggg
      481 cgatggatat aaaaattggc aaaatgagct tgaaagaatt gggactattg gtatatttga
      541 aaaaaataca tcagaagaaa aaaatctaaa attataaaat gtgatactca tactaaaata
      601 ggagtttcaa gtgatggaaa atacgattgc tatttaagca caaatagcgg agctgaaagt
      661 aatcttctgg atgaattaaa aagaactgag attcaaatta tagataaaaa agaacgtcct
      721 aaaaatggtt ttgtgctatc agaaaaaact gatttagaaa atacagaagc atttaacaaa
      781 gaatcggtta aagatttacg taaactacca acaaaggaca taaatggcaa ggattttaca
      841 agtaaagatt ttgagaagta tgatcttact atggtaaatg tatttgcaac ttggtgtaca
      901 gcttgtgtaa aggaaattcc tgatttggtt gaggttcaga atgaaatgaa aagcaagggt
      961 gtaaatatcg ttggagttgt tacagatcct gttgatgata acggtgaaaa caaggaagca
     1021 attgaaaagt caaagttaat acatgaaaag acaaaggctt catatccttt cttaatgcca
     1081 gatggaatgg caacactttt tttgacaaaa attataaggt gttgtgaatg ctgcggtgtt
     1141 atggct
//
LOCUS       DAYYAR010000275         1143 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000275 DAYYAR010000000
VERSION     DAYYAR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.281"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..1073
                     /gene="yidC"
                     /locus_tag="ACHAT0_002939"
     CDS             123..1073
                     /gene="yidC"
                     /locus_tag="ACHAT0_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8452820.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT          388 a          227 c          239 g          289 t
ORIGIN      
        1 aaaatttttc gctacgaaaa agaacgaata tttatatgaa atttctatga agttgattga
       61 attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt
      121 ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt
      181 tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcagga atcgtttatc
      241 gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgagaat ttcaattggt
      301 cttacggctg ggcagttatt gcaatgactg tgatcgttcg tatcatcatc ttgccactag
      361 gtatcagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac
      421 aagttgaagc agcacagcaa aagctaaaag ctgcaacaac acgtgaagaa caaatggcag
      481 cacaagcaga aatgcagcaa gtatatcgcg aaaatggatt gagtatgacc ggcggaatcg
      541 gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtatttt acggcacgct
      601 acacagaagg tatccgggaa tcatcttttt acggcatcga tctaggctca ccaagtatgg
      661 tgcttgtggc catcgcaggg gtcgcttatc tgcttcaagg atatatttca acaatcggta
      721 ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct gctatgatcg
      781 tcttcatgtc aatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt
      841 tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag
      901 tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt cactccaaga aaagacgtga
      961 cagaatcagc aaagccagct aacaacaaac aagcaacaaa acaaatcaaa caaccaaaaa
     1021 acagtacaaa caacaaaggc cgcaacgcag gaaagcagca gaagcggaag tagatagtaa
     1081 ataagagtgt aataaaaatt cattaacagt tttagcaatt aaaaatatcg accaagattt
     1141 ttg
//
LOCUS       DAYYAR010000276         1134 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000276 DAYYAR010000000
VERSION     DAYYAR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.282"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(405..779)
                     /locus_tag="ACHAT0_002940"
     CDS             complement(405..779)
                     /locus_tag="ACHAT0_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8452821.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(802..>1134)
                     /locus_tag="ACHAT0_002941"
     CDS             complement(802..>1134)
                     /locus_tag="ACHAT0_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFM8452822.1"
                     /translation="LKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQMFDKGLQR
                     PLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGAIKVDKLDY
                     SINYECGF"
BASE COUNT          386 a          212 c          137 g          399 t
ORIGIN      
        1 taggagtgag catttttcga aaaatactaa aaatttttca aaataaaaaa aggcatacag
       61 acaataaact gtctatacgc ctcacagact ctattctata catctaattt tggctgtcct
      121 gcaaaaaata tgtgttagcc tccatcgaaa gatcaaactt tggaaaacat cattataact
      181 ttgcttggta agagctttgc taaaacatga tatgacttgt atacaaaatg tggagtgtat
      241 tccaataaat aggaaggatt acaaaatttg aaaccaaata agtaaaaaat actgtcactt
      301 tgaattaatt tgaaatagta aattctcatt aaactattga aatgctgata ccaaaaaatg
      361 aagtactttt cgttcatacc aacttagagc acttcgttat ctatttattt cttcatttgg
      421 cttatctcta taatctcatg atttggtccg tagaaattaa agtaacggat tccattttcc
      481 cagaacggta attccataat atttttatct aataattcat atcctaaatc ttgaattgaa
      541 ttgaatacct catctatgtc atccgtatct aatgctagat gatcaatggc tcctctacac
      601 tcagtagttt tatcttccca tatttctatt atcgttccat tcaactcgaa aaagccgact
      661 ttacttccca cattcatttc ttcattaatc agactaaacc ctaaggattc ataaaagatt
      721 tttgaagctc caaaatcttt tgttggaatt ccaatatgtt gtaaaccatt taatttcact
      781 tacatcatcc cttcaattgt tttaaaatcc acattcataa tttattgaat agtctagttt
      841 atctaccttg attgctccct ccgtaacagt catacttaat gctgttccga ctttatcata
      901 cattctagta cttaaagcaa cattatcaat atataaaaca gctatcgtat catcaacaat
      961 taactttagt tcatattctt tgcctatttc caaaaatagt ggtcgttgca gccccttatc
     1021 aaacatttga ggccaaggat agttcggagt ttttccgaaa gtaagctgat tattaatcaa
     1081 tgaaatacga aattcgtatc cttcttgagt atcactatct acaaataatt ttaa
//
LOCUS       DAYYAR010000277         1128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000277 DAYYAR010000000
VERSION     DAYYAR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.283"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..151
                     /locus_tag="ACHAT0_002942"
     CDS             <1..151
                     /locus_tag="ACHAT0_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase DegT"
                     /protein_id="HFM8452823.1"
                     /translation="PVFKNYDYIGDDVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKR
                     VINDA"
     gene            141..761
                     /locus_tag="ACHAT0_002943"
     CDS             141..761
                     /locus_tag="ACHAT0_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8452824.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
BASE COUNT          436 a          133 c          202 g          357 t
ORIGIN      
        1 acctgttttt aaaaattatg attatattgg cgatgatgta gcagaaactt tatttgactt
       61 aggagtatgt ttaccatcag atacaaagat gacagatgaa gatttaaaat atgtagtatc
      121 tacgattaaa agagtgatca atgatgcgta aaaaaacttt ctatcagctt tttgggaaaa
      181 gaattttaga tatcctattg tcaggaattg ctctcatcgt tttatcgccg ataatcttaa
      241 ttgtaggttt tttagtaaga attaaacttg gttctccaat tatcttcaaa caagaacgac
      301 caggaaaaag tgaaaaaatt ttttccatgt ataaatttcg tacaatgact gacgaaagag
      361 accacaatgg agaatattta cctgatgaaa ttcgactaac taaatttggg aaaatgctta
      421 gagcaacaag tctggatgag ctacccgaac tttggaatat tttgaaaggt gatatgtcga
      481 tcgtaggacc tcgtccatta ttagtggagt atttgccact atatagtgag aagcaacgga
      541 aaagacacaa cgtaagacca ggattaactg gactggctca ggtcaatgga agaaatgcta
      601 ttagttggga agaaaagttt gatttagatg tttattatgt tgataaaatt agtttcttta
      661 atgatcttat tattataatt cagacatgta aaaaagtaat caagaaagag aatattaata
      721 caatcaataa caatatccca gaaaaattta aagggagtta aatacagcag cttaatgcaa
      781 gttttgtgac ttgcattata tttaggtata aatcaaagcg agtatattag aattatataa
      841 catatgttaa aaaaacgtat agctaataaa agtatgattt taaaaatttg tgtagagttt
      901 taaatatact gtttcaatac gagaaaatta taaaaaagta taaggaaaag agagttatgg
      961 caaagtatta taataaaaaa attcttataa tggcaaacaa cgatgatggt ctttaccgtt
     1021 ttcgaggaat gctcataaaa aacttagtag atcaaggtaa tgaagttatt gtatctcttc
     1081 caaagggcaa gaatattcct aaattaattg aacttggatg tcaaatta
//
LOCUS       DAYYAR010000278         1125 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000278 DAYYAR010000000
VERSION     DAYYAR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.284"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..493
                     /locus_tag="ACHAT0_002944"
     CDS             <1..493
                     /locus_tag="ACHAT0_002944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452825.1"
                     /translation="WGENMSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQK
                     SILTEFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIF
                     SSISIIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKND
                     ESY"
     gene            complement(552..722)
                     /locus_tag="ACHAT0_002945"
     CDS             complement(552..722)
                     /locus_tag="ACHAT0_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452826.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(722..1021)
                     /locus_tag="ACHAT0_002946"
     CDS             complement(722..1021)
                     /locus_tag="ACHAT0_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452827.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
BASE COUNT          337 a          211 c          165 g          412 t
ORIGIN      
        1 gtggggtgaa aacatgagca gaaaaattga taatataata ttgttaacaa ctatttttta
       61 tttagtaatc ggagcaactc tttccttact gtcatttttg tccaacagta gtttgattgt
      121 gttaaatcaa aaaagcatat taacagaatt tgcccacata gattacttct ctttttttaa
      181 aactggacta atatgtttat ttctatcttc atttatcctc tatataggaa ctacattttg
      241 tctaatggtg cttaatttaa aaatgtttat aattgaaaaa tggatagtta ttatcgcaaa
      301 gattttctca tcgatatcaa ttatcattcc aatgacattg acgttttcta aagagcaatt
      361 taacgtagct actacattcg tatcttttat agctctattt tcgtttgctg ttcctaaaac
      421 ttttcaaagc aaatctagca acgcaaaggt taatgtagaa aacactgata gaaaaaatga
      481 tgaaagttat taaaaaaaat aattctttca tttattgtca aacctttctt atagacatta
      541 gactttttta gtcagtccct cccgactggc ttttttgttt tgtactcagc ttcatccagc
      601 cctataaaaa tccaaaccat gtacacaatc gtacctatca acgcttgtct gcttccccag
      661 aggcctaaag cgtagatgat tagcggtgcg ctgaatacta gtgctctgtt gaatttgccc
      721 attaacttgc ctccttcaaa acatctgaag gcgataaact ttttacacga tatttgtttg
      781 cgtctttcca ctttaaaaac caaataaaag tatggtaatg aataaatgtg gttgagtgac
      841 ctggacgaag tattccttct gcaaattcag gaattgtttc catttccttg cagtattcaa
      901 ctaaagtagt ttttgacatg ccgtggaact tctccaagat taaactttgt cggtaccagt
      961 cgtctggttt tactgccttc tcagcagctt ctatcagctc tgaaagtgtt ggttttttca
     1021 cttattatcc ctcctaaaaa ttatcgttcc atactcgatg tttaacattt cttgaagctc
     1081 tgattttgta agaatgattt tatcttccaa cgactttacc tccct
//
LOCUS       DAYYAR010000279         1101 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000279 DAYYAR010000000
VERSION     DAYYAR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.285"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1014)
                     /locus_tag="ACHAT0_002947"
     CDS             complement(<1..1014)
                     /locus_tag="ACHAT0_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8452828.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRT"
BASE COUNT          324 a          237 c          184 g          356 t
ORIGIN      
        1 tgtacgaatc cttcggcgga tattttcagg aaaagacaaa atttgaatag catcttcaaa
       61 gccgttttct aaaatgtcgc aagcagcact taattttgaa tcacaaccat atttttccaa
      121 gaagtgctct tttctttcac gtgtcaattc aagttcagag gctttaaaga tactcttcaa
      181 ctcgtctttt acatcagaag aaactttctt tggcagctta tcaaaaatat ttctcaaaaa
      241 gtgggcttgg catctttgcc agctcgcacc taaaaagttc ttgcgaacag ccttaactaa
      301 ccctttgtgt gcatcagaaa tgaccagttt tacaccgaac aaacctcgtt gaacgaacga
      361 ttgaaaaacg gtatcccaat tatcttccga ttcgccgtct tgaacatcaa aaccaagaat
      421 cgtacgtcgt ccatcagaat caatgccgat accaatatat acaccttttg agacaatacg
      481 atgattttcc cttactttca tatacagggc atcacacatg aaaaatggat attccgagcc
      541 ttctaggctc cggtttctcc actcaaaaac aaatggatcc aactgtttca tcagatttga
      601 aacgaatgat ttagaaacag tcgcaccatc cgttagcagt tcaactgttt tagttacttt
      661 gcgagtggag acaccagaaa tgaccatttc aatcatggca gcgatcaaag ctttttcgct
      721 tcgctgatat ctttcaaaga tagtaggact aaacttaccg tctctggttc gtggaacatg
      781 aagttctaac gtaccaattc gagttgtaaa cgaacgatcg tagtaaccat ttctttgtga
      841 ttgccgttct tctgaacgtt cataggcatt tgcctgaata tattgatttc gttgttcttc
      901 catcagctga ttaaacatga tagtcataag tgtttttgcg gtatcattcg caccgctttc
      961 gtttataatt gcttgaattt cttcgctttc aaaagtaaaa tgtacttgag tcattgatat
     1021 cagtcctatt cgagtatgtt ttctgcaaaa aacattgtac tcctaactga gatcaatggc
     1081 ttttttgatg cttcactttt a
//
LOCUS       DAYYAR010000280         1088 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000280 DAYYAR010000000
VERSION     DAYYAR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.286"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1088
                     /locus_tag="ACHAT0_002948"
     rRNA            <1..>1088
                     /locus_tag="ACHAT0_002948"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          287 a          250 c          332 g          219 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagc
//
LOCUS       DAYYAR010000281         1087 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000281 DAYYAR010000000
VERSION     DAYYAR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.287"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            227..568
                     /locus_tag="ACHAT0_002949"
     CDS             227..568
                     /locus_tag="ACHAT0_002949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452829.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
BASE COUNT          397 a          119 c          183 g          388 t
ORIGIN      
        1 taaatgtaac tggaaaagaa tgtatttcta tattgatggt gatgatgctc ttacattcgg
       61 taaaactact ttttctagtt ttaattttca gtttctatta aaaaataatt tttatggttt
      121 attgaaataa ggttgcgaag gaagatgtag agttatttgc actgttgtgt tggtatatct
      181 cttaatgctg tgtctttttg gggagattaa ttagtggagt gattatatga gtatagatga
      241 ttgtttagaa gaacaaataa gattattttt aaagttttta aaagttaaag ttcaaaataa
      301 ctacttcaca tgttatagtt atgcacaccc tgttttaaat agtcttcctt ttaattcttt
      361 agtgcaagga ttgattacaa atattgtttt attagtctgt acaccaaatg aaatatttgt
      421 aaaaaagata ggcagtggta tttcttcagt aagattaaaa gagaaagaat atactcaaaa
      481 gattattcat attcctaagc ctgaaattaa gcgttttgag ataaaagact attctgcttt
      541 agggctgttc tcaattggtt actggtgata aaaaataaga atactaaaac tatacgtaga
      601 atataagtaa tcggcatcaa tcctaattat gtgattgaat gccgattttt gttacgcgct
      661 atttttaatt atctgtaaga agaataattt tattttactc atattcgttt tcactttgaa
      721 gaagtaaatt gcaatttctt ataagttaag cagctatgca aaaggctatt tattctattg
      781 tgaaatataa gatgtagttg aaacaggata tatagaatca aaatacgaat cttattttga
      841 tttttctagt tttttgaaaa aagcaccctt tgatcgttgt attagtggaa aagagaaaaa
      901 ggagcatact tatcatgtca caaaactaca actaaatatc taaacaacac caatttgatg
      961 cctattgaaa aaaagtattg cgaaatgaag ccaagtctat tagaaaatga aataatagaa
     1021 taaaagaaaa tgggaaaccg ctgaatgatg taaatgaagg taagtatcca cgaactaatt
     1081 ttgatga
//
LOCUS       DAYYAR010000282         1082 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000282 DAYYAR010000000
VERSION     DAYYAR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.288"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..132)
                     /locus_tag="ACHAT0_002950"
     tRNA            complement(48..132)
                     /locus_tag="ACHAT0_002950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Leu,seq:caa)
     gene            complement(153..223)
                     /locus_tag="ACHAT0_002951"
     tRNA            complement(153..223)
                     /locus_tag="ACHAT0_002951"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(189..191),aa:Cys,seq:gca)
     gene            complement(269..340)
                     /locus_tag="ACHAT0_002952"
     tRNA            complement(269..340)
                     /locus_tag="ACHAT0_002952"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(306..308),aa:Gln,seq:ttg)
     gene            complement(352..424)
                     /locus_tag="ACHAT0_002953"
     tRNA            complement(352..424)
                     /locus_tag="ACHAT0_002953"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(389..391),aa:His,seq:gtg)
     gene            complement(427..500)
                     /locus_tag="ACHAT0_002954"
     tRNA            complement(427..500)
                     /locus_tag="ACHAT0_002954"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Trp,seq:cca)
     gene            complement(507..587)
                     /locus_tag="ACHAT0_002955"
     tRNA            complement(507..587)
                     /locus_tag="ACHAT0_002955"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(551..553),aa:Tyr,seq:gta)
     gene            complement(589..664)
                     /locus_tag="ACHAT0_002956"
     tRNA            complement(589..664)
                     /locus_tag="ACHAT0_002956"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(629..631),aa:Phe,seq:gaa)
     gene            complement(700..772)
                     /locus_tag="ACHAT0_002957"
     tRNA            complement(700..772)
                     /locus_tag="ACHAT0_002957"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(737..739),aa:Asp,seq:gtc)
     gene            complement(782..854)
                     /locus_tag="ACHAT0_002958"
     tRNA            complement(782..854)
                     /locus_tag="ACHAT0_002958"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(819..821),aa:Val,seq:tac)
     gene            complement(870..941)
                     /locus_tag="ACHAT0_002959"
     tRNA            complement(870..941)
                     /locus_tag="ACHAT0_002959"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(906..908),aa:Glu,seq:ttc)
     gene            complement(956..1045)
                     /locus_tag="ACHAT0_002960"
     tRNA            complement(956..1045)
                     /locus_tag="ACHAT0_002960"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1009..1011),aa:Ser,seq:gga)
BASE COUNT          303 a          283 c          247 g          249 t
ORIGIN      
        1 ttatttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac cggcggccgg
       61 ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt ctgccaattc
      121 cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg aaccggtgat
      181 gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac tattatcaat
      241 ctataaaata aagaataaat tatagaaact ggggtagctg gattcgaacc aacgcatgag
      301 ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaaaa gggcgaatga
      361 tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac ttcgccacat
      421 tcgccatggc aggggcagca ggaattgaac ccacactaac ggttttggag accgctgttc
      481 tacctttaaa ctatgcccct ataaaatgga ggagagtgga ttcgaaccac tgaaccctaa
      541 ggaacggatt tacagtccgt cgcgtttagc cacttcgcta ctcctccatg gtggcgcggg
      601 acagaatcga actgccgaca cacggagctt caatccgttg ctctaccaac tgagctaccg
      661 cgccatattt taaaattatt aattgaataa caagaacgac ggttccgacg ggaatcgaac
      721 ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa ccagttcatt
      781 atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg ctctcccagc
      841 tgagctaaac ctccaacatt caattaaaat gacccgtacg ggattcgaac ccgtgttacc
      901 gccgtgaaag ggcggtgtct taaccgcttg accaacgggc caggtaaatt tgctacggag
      961 agtaagggat tcgaaccctt gagacagttt ggcaccatct acatgatttc caatcatgct
     1021 ccttcggcct ctcggacaac tctccagagt gaaagaagct caatactctt tctcaaaatg
     1081 ag
//
LOCUS       DAYYAR010000283         1068 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000283 DAYYAR010000000
VERSION     DAYYAR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.290"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(169..564)
                     /locus_tag="ACHAT0_002961"
     CDS             complement(169..564)
                     /locus_tag="ACHAT0_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM8452830.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            complement(557..928)
                     /locus_tag="ACHAT0_002962"
     CDS             complement(557..928)
                     /locus_tag="ACHAT0_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452831.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
BASE COUNT          331 a          201 c          133 g          403 t
ORIGIN      
        1 gaaacatttt gaatatcaat tcctacattt tcagttccag attccattac tgataaaacg
       61 aaataaaaat ctttagttga ctgcataact gaatcaagat atattgtccc atcaggatag
      121 atttctttta atttgctact gattgcagca atgatttcat ctttcatgtt actttccttt
      181 cttaacgatt tttattgcca tttgtttaaa tcttcgagga atataagaca tatttgcaag
      241 attcgttgca cgagttaaca tgaatttccc tttgacaaag ccaccatttt tagtcctatg
      301 accttcttct acatatttaa aataatgttc attgtttata acttctccaa caattcgacc
      361 gcttgatagt tttctagctt ttactacacg atatcctcgc cttaaattac ctgattttac
      421 aggagttaat ggtttagcta aactaacgac tttattcatt gaatcgttaa cgaaggatat
      481 tccttctttt tgagcaatta cagtcatatt tttaaaattc tcaatgatct tttctgcatt
      541 tgattcatat ttgagattac ccatttttct cacttcctat caaattcact tcacaatgac
      601 taggatagta aaaaggctta gtagcaaata gagaaaaaat taagccactg gccttttgag
      661 ttattgttat tcgatctcct ttttttagtt taatcttagg atgaacaaat aatttatatg
      721 tgtcagttga aacatttacc atctctccat cttctatgac tgctagtcca tcgatttgac
      781 cttgtgaaag agcgcaagaa ataggattat catatacttt tttgtaatct tgaatagtaa
      841 tatttgtatt agaatcttca atgtccgtca gtctttcaat aatacaacta tcttcataag
      901 tagtttctaa aatatctgct tcattcatta aaaaaactcc aatcctccac aaccaataac
      961 tcttcgtatc aaatcaccat agcctagaag taactcacta acatcatttg ttgttgaagc
     1021 ataactaatc gttgtatcgc cacgtctaac tgaagatact gttttttc
//
LOCUS       DAYYAR010000284         1043 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000284 DAYYAR010000000
VERSION     DAYYAR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.291"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACHAT0_002963"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACHAT0_002963"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8452832.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          374 a          132 c          182 g          355 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg taggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aac
//
LOCUS       DAYYAR010000285         1013 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000285 DAYYAR010000000
VERSION     DAYYAR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.292"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..>1013
                     /locus_tag="ACHAT0_002964"
     CDS             141..>1013
                     /locus_tag="ACHAT0_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8452833.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKKGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          351 a          179 c          180 g          303 t
ORIGIN      
        1 ttcgaacatc atcactctca tctacttaat ttcgcatttt tactttacgc cgattgtaaa
       61 attaagctag acaactaaaa aagtcatttg tgctacactc aaaatagttc cgaccaaaga
      121 attataagga gtgagtacaa atgacctata cccatcttac aacggatgaa cttgtaataa
      181 tagagtctta tttcaaaatt aatcaatctg ttgctaaaac tgcccattgc ttgaatcgtt
      241 caagacaaac gatccataaa gtatatctgt tcttcaagaa aggaaaatca gccttagaat
      301 attatcaaca gtataagaaa aacaaatcaa actgtggtag acgtccgctt gttttacccg
      361 aggaacaatc agaatatatt caaagaaagg ttgttcaagg atggacaccc gatgtgattg
      421 ttggtcgtgc agcgtttcct attagttgtt ctgctcgtac catttatcgt atgttcaaaa
      481 aggggctatt tgattcttct gacttaccga tgaaaggcaa gcgtaaaccg aatggacatc
      541 aagaaagacg tggaaaacaa actttccgcc gctctattca tgaacgtgaa aaggattata
      601 gccaattctc aaatgagttt ggtcaccttg aaggtgacac tatcgtaggt ctgaaacata
      661 aaagtgctgt aattacctta gttgaacgat tatcaaaagt tatcatcaca ttgaaaccgt
      721 gtggtagaca agcgattgat attgaaaaaa aattaaatca atggtttgaa tctgtaccga
      781 aaaacctatt caaatccatc acttttgatt gtggaaagga attttcaaat tggaaacaga
      841 tcagtaatgt caatgatatt gccatttatt tcgctgatcc aggaacgccg tctcaaagag
      901 gcctaaacga gaattctaac ggattgttac gtagagatgg tttattgaaa tctatggatt
      961 tcaattcagt agatgaattt tttattcaat ctgtcgcatc taaacgaaat aat
//
LOCUS       DAYYAR010000286         1001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000286 DAYYAR010000000
VERSION     DAYYAR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.293"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1001
                     /locus_tag="ACHAT0_002965"
                     /pseudo
     CDS             370..1001
                     /locus_tag="ACHAT0_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DAYYAR010000287          991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000287 DAYYAR010000000
VERSION     DAYYAR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.294"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..94)
                     /locus_tag="ACHAT0_002966"
     tRNA            complement(21..94)
                     /locus_tag="ACHAT0_002966"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ACHAT0_002967"
     tRNA            complement(108..181)
                     /locus_tag="ACHAT0_002967"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ACHAT0_002968"
     tRNA            complement(209..281)
                     /locus_tag="ACHAT0_002968"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ACHAT0_002969"
     tRNA            complement(301..374)
                     /locus_tag="ACHAT0_002969"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="ACHAT0_002970"
     tRNA            complement(384..457)
                     /locus_tag="ACHAT0_002970"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="ACHAT0_002971"
     tRNA            complement(468..553)
                     /locus_tag="ACHAT0_002971"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="ACHAT0_002972"
     tRNA            complement(570..641)
                     /locus_tag="ACHAT0_002972"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="ACHAT0_002973"
     tRNA            complement(653..725)
                     /locus_tag="ACHAT0_002973"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="ACHAT0_002974"
     tRNA            complement(743..824)
                     /locus_tag="ACHAT0_002974"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="ACHAT0_002975"
     tRNA            complement(832..904)
                     /locus_tag="ACHAT0_002975"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="ACHAT0_002976"
     tRNA            complement(908..980)
                     /locus_tag="ACHAT0_002976"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DAYYAR010000288          990 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000288 DAYYAR010000000
VERSION     DAYYAR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.295"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            777..>990
                     /locus_tag="ACHAT0_002977"
     CDS             777..>990
                     /locus_tag="ACHAT0_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8452834.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDI"
BASE COUNT          362 a          162 c          124 g          342 t
ORIGIN      
        1 ctgaattcgg aattaaaaac cacgataggt aactttaaac aaacatgaag agactttaat
       61 aagacttctt tatctgaaaa cacttttgtt tctctctttc ctttctacat cattaaatag
      121 tattttatac gttatctaaa atactattcc tctgtcaaaa taagctaact tacttcatta
      181 taaaatatcc acttatcgct ttcatgtata ttccaccgta aaaagtacat tttatcaccg
      241 agaactcatc atttttcatc atgatgaata ctatttacat atatctagtt caaagacaat
      301 aattattatg atccgttatt atctaagcac tatacagata ataggaattt ttcgtttcta
      361 aatactccaa tttgggaatc caataaaaat tttcgttcaa atacgtgtct attcgaactc
      421 ttttctagtt gttaattatg aagggggaag aaataaaaaa tacaatctat acacgaataa
      481 gaatattatc caaaaggagg ttttagaaaa gatttattat acgtaaaaag ctcaagacat
      541 tttcttagcc ataaggacat gtttgatatt taagaagata tatgaatttt cttttctaag
      601 tataaagata actatccatt atcaactaaa aaaacatttt tgaaaataaa catcagcagt
      661 cctatcaatg catgtgagct tatagaagtt attcgcagcg cagcataatt gcattagctt
      721 cttttcgtgt tttttgcctc ttttcccttt aaatcttaat ggaggaggtg tgcaatatgt
      781 cagataacga cgaacgcgta aatgcccagt tgattcgatt ctttgaaaag gtcattttga
      841 acaccgctaa tacttattat aaaaaacaag aagaaattca aaaacatgaa caacttgatc
      901 aactcccacc atatttgttt actaaagata ctataaaatt taaacaaccg attaatgttt
      961 taaatgtgac tctccttgtt gaagatataa
//
LOCUS       DAYYAR010000289          982 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000289 DAYYAR010000000
VERSION     DAYYAR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.296"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>982
                     /locus_tag="ACHAT0_002978"
     CDS             82..>982
                     /locus_tag="ACHAT0_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8452835.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEY"
BASE COUNT          335 a          183 c          192 g          272 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata ca
//
LOCUS       DAYYAR010000290          972 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000290 DAYYAR010000000
VERSION     DAYYAR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.297"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..965)
                     /gene="scrK"
                     /locus_tag="ACHAT0_002979"
     CDS             complement(93..965)
                     /gene="scrK"
                     /locus_tag="ACHAT0_002979"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8452836.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
BASE COUNT          298 a          211 c          138 g          325 t
ORIGIN      
        1 tttttttttc acttttaggc gtcccttcaa ttactaattg aaaaaaagat cgcatcaacc
       61 aaacaaatag tcgagacgat cttttttcca aattaagatt tttctttaac gtctcttgct
      121 aaagctaaac aacctacaat accagaatta tctaacaatt ttggtgtcat aatataattg
      181 tcaacatcgg gaatagcgac atatccgttg atcaactcaa taaacttatc ttgtactttc
      241 ttttttaagt gtttttgttt cattactccg ccaccaaata taatgacatc aggtgataac
      301 atcaatgttg tattatatgc acactgagca atgtaataag cttctatttc ccaagtaacg
      361 tcattttcag tcacttcttg tccctttttc cctgatcttc tttcgattgc cggaccagca
      421 gctaagccct ctaaacagtc tccgtggaat ggacatgtcc cagaaaagtt gtctttattg
      481 tgtcgtttta caagcatatg tcccatttct ggatgactaa aaccttcaat gaagtttccg
      541 ttttggatag cgccacctcc tatgcccgtt cctacggtat agtaaacaac acttgaaagg
      601 cctctgccat ttccagaaac atattcacca tagcaagagg catttacatc agtcgtccaa
      661 gcagttggaa tattaagttg cttttttaaa cttccaataa aatcaaaatc ctgccaagca
      721 agttttggcg ttgatttaat gaagccatac gttttagatt cacaatgaat atcaatagga
      781 ccgaacgatc ccactccgat accttctaaa tcatctttat gatgattaaa aaaatctaaa
      841 actaaattca ttgtttcttt gggtgtagtc gtgggaaagc tgactttttc aaatatgttc
      901 aaatctttat ctccaatggc acaaacaaat tttgttccac cagcttcaat agccccaaaa
      961 accatacatt tt
//
LOCUS       DAYYAR010000291          959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000291 DAYYAR010000000
VERSION     DAYYAR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.298"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACHAT0_002980"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACHAT0_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ACHAT0_002981"
     CDS             complement(570..>959)
                     /locus_tag="ACHAT0_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8452837.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAYYAR010000292          952 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000292 DAYYAR010000000
VERSION     DAYYAR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.299"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..887
                     /gene="galU"
                     /locus_tag="ACHAT0_002982"
     CDS             6..887
                     /gene="galU"
                     /locus_tag="ACHAT0_002982"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8452838.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          345 a          161 c          220 g          226 t
ORIGIN      
        1 agcaagtgaa ggtcaaaaaa gcagtcattc ctgccgctgg gctaggaaca cgttttttgc
       61 cggcaacaaa agccatggca aaagaaatgc ttccaatcgt ggataaacca acgatccagt
      121 ttattgtgga agaagcattg gattcgggaa tcgaagacat cttgatcgta acgggaaaag
      181 aaaaaagacc cattgaagat cactttgatg caaatatcga attggaaaac aatttgcggg
      241 aaaaggaaaa aacagaatta ctcgctttag ttgaagaaac gactcaagtc aatcttcact
      301 tcatccgtca atctcatcca agaggactgg gggatgccgt gcttcaagca aaagcattta
      361 taggaaatga accgtttgtc gtcatgctgg gggatgattt gatgaaagat gatatcccat
      421 tgactcaaca attgatgatg gattatgaag aaacacaagc ttccgctgtt gccgtcatgc
      481 gtgtaccaga aaatgaaaca tctaaatacg gaattattga tcctaaaaca gatagcgaaa
      541 agggtcgttg cagagtaaaa gggtttgtag aaaaacctga aatgggaaaa gcaccaagta
      601 atttagccat tatcggacgt tatctactga ctcctaaaat atttgagata ttagaaactc
      661 aagaaccagg agcaggaaac gagatacagc taacggatgc acttcagact cttaaccaga
      721 cagaggcggt ctatgcgaga gagtttaaag gaaaaagata cgacgtaggc gataagttag
      781 gatatatgaa gacaaatatt gaatatggac ttcaacattc ggaaattggg acttcattaa
      841 gtgcatatat tatccagttg agtaaaaagc taagaacaga aagttaactg gaaataagtg
      901 gcctgctgag aatgaaaggc tatttgttat taaagcggca gcttgtgcgt ca
//
LOCUS       DAYYAR010000293          950 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000293 DAYYAR010000000
VERSION     DAYYAR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.300"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>950)
                     /locus_tag="ACHAT0_002983"
     CDS             complement(37..>950)
                     /locus_tag="ACHAT0_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452839.1"
                     /translation="LKDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFF
                     SDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFE
                     NQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYI
                     NDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNL
                     SLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLT
                     IQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          308 a          162 c          145 g          335 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca
//
LOCUS       DAYYAR010000294          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000294 DAYYAR010000000
VERSION     DAYYAR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.301"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHAT0_002984"
     rRNA            <1..>943
                     /locus_tag="ACHAT0_002984"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYAR010000295          940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000295 DAYYAR010000000
VERSION     DAYYAR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.302"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACHAT0_002985"
     CDS             <1..459
                     /locus_tag="ACHAT0_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452840.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACHAT0_002986"
     CDS             473..904
                     /locus_tag="ACHAT0_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452841.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          358 a          135 c          197 g          250 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga
//
LOCUS       DAYYAR010000296          924 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000296 DAYYAR010000000
VERSION     DAYYAR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.303"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..320
                     /locus_tag="ACHAT0_002987"
                     /pseudo
     CDS             18..320
                     /locus_tag="ACHAT0_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            428..>924
                     /locus_tag="ACHAT0_002988"
     CDS             428..>924
                     /locus_tag="ACHAT0_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8452842.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          343 a          140 c          159 g          282 t
ORIGIN      
        1 taagaaggag tataaaaatg aacaaacata atagaaaaat aacgaataac aaagaactga
       61 tcttgttact aatagggatt tggggtatct ttttactctt taatatgtat atgccgacta
      121 tgagagcgga tgacgtagtt tatgcaagta gattagatga acttggctat ttgggtgctt
      181 cgatagaaca ttataagaca tggagttcaa gaattattat agaactattc ttaatgttct
      241 tttctaaaca ctttatgcta tggaaattat taaattctac gattatgtta ggaattgtag
      301 tattactttg caagtatgtt gagagtataa aatattttgt gtaaatgaaa aaatccatac
      361 aaaaaaggaa gtcccttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga
      421 cttccctatg aatgatttta ctacagaaat tgtgcaaact ctagtcacta aaggcgattt
      481 aaatgaatta ttccgttcgc acttagaaaa agcgataaac acactcctac ggactgaatt
      541 aacggctttt ttagattacg aaaaatatga tcgcactggt tttaattcag gtaattcgag
      601 aaacggttct tactttcgat caatcaaaac cgaatatggt gaattaacat tggaaatacc
      661 tagagatcgt aatggtgagt ttaaacaaca aactttacca gcctacaaaa gaacaaacga
      721 tacattggaa accactatta tccatttatt cgaaaaaggt gttacgatgt ctgaaattgc
      781 tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt ccaacatgac
      841 taaagttctg actgaagaag taaatgcctt taaatccaga gccttaaatg ataagtatgt
      901 cgctattttt atggacgcta ctta
//
LOCUS       DAYYAR010000297          920 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000297 DAYYAR010000000
VERSION     DAYYAR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.304"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..>920)
                     /locus_tag="ACHAT0_002989"
     CDS             complement(56..>920)
                     /locus_tag="ACHAT0_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8452843.1"
                     /translation="GKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPEL
                     EEWQARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTE
                     SKNRWMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRY
                     LPSKDYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFD
                     YGSAIRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFM
                     KTNARSDSYDV"
BASE COUNT          285 a          194 c          163 g          278 t
ORIGIN      
        1 aactagatca tcaatcgtaa gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga
       61 catcatagct atcactcctt gcattcgttt tcataaatct attataaaag aaaagactgt
      121 agacatcatc cataaaatgg actttgtcta cagtctgcgc attctcatta gagaacactc
      181 ccttttttgt gacttttctg aagctagagt gaatactttc aactgcatta gtagtgtaca
      241 tgatttttct aatggcgctg ccataatcaa agagttgttc aacatgagaa aagtttcgtt
      301 tgcatacatc tactgcacca gaataatgag accaccgatt ttgaaaactg tcgaaggcag
      361 cgtgtgcagc gtttaaagaa gaagcactgt agaatttttt catgtctcga cagacttcct
      421 tataatcctt acttgggagg tatcgtaacg catttcgaac aagatgaaca atacagcgct
      481 gcacaatgac agaaggaaaa attgcttttg cgccttcttt gagaccggaa acaccgtcca
      541 tcgaaatgaa aaaaacatct tctacaccac gtactttcag ttcatcaaat acttgcatcc
      601 agcgattttt agattctgtt tgattgagcc ataggcctaa aatctccttg tttcctttaa
      661 gatcatagcc aagaatggtg tacacagcat attctttagc ttcatagttt tcgcgtaaag
      721 tgacgtacat acaatcaacg aatagaaatg cataacactt ggctaatggc cgagcttgcc
      781 attcttccaa ttcaggaagg acggcatccg tgatatcaga aatcatttcg tgggaaatat
      841 caaagccgta aatatcttca acggttgcgg cgatatcgca ttgactcatt cctcgtgcat
      901 acatggaaag aaccttacct
//
LOCUS       DAYYAR010000298          914 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000298 DAYYAR010000000
VERSION     DAYYAR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.305"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..432
                     /locus_tag="ACHAT0_002990"
     CDS             <1..432
                     /locus_tag="ACHAT0_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8452844.1"
                     /translation="RICFVDKLCYYYRQQNNSIMHKPKSNLVFRHGNMIADYEKLIRQ
                     NGNNIIDKKACMLVAFDVLIMEIFNQKNVGGTCEDYKNALVSLKHHKILRENISNIVR
                     MKAYELEPNMRKKLALVTISIMFNFKMYKTAYNMLWRIYNR"
     gene            446..>914
                     /locus_tag="ACHAT0_002991"
     CDS             446..>914
                     /locus_tag="ACHAT0_002991"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8452845.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKIND"
BASE COUNT          363 a           96 c          173 g          282 t
ORIGIN      
        1 agaatctgtt tcgtagataa attgtgttat tattatcgcc aacaaaataa ttctataatg
       61 cataagccaa aaagtaattt ggtttttaga catggaaata tgattgctga ttatgaaaag
      121 ttgataaggc aaaatggaaa caacataata gataaaaaag cttgtatgtt ggttgcattt
      181 gatgtactaa ttatggagat ttttaatcaa aaaaacgttg gtggaacatg tgaagattat
      241 aagaatgcat tagtttcttt gaaacatcat aaaatattga gagaaaatat ttcaaatata
      301 gttagaatga aagcatatga acttgaacct aatatgagaa agaagttagc ccttgtgacc
      361 attagtataa tgtttaattt taaaatgtat aaaacagcgt ataatatgct ctggagaata
      421 tataatcgat aaagaggtag gaaatatgag gaaattgacc tttgtgatag gaattatggg
      481 aaaaggaggg gcagaacgag taattgcgaa tttggcaaat aattttattc aagataactg
      541 ggaaattgat attataacta tatatgggaa tcggcaagac tatgaactga atagaaaaat
      601 acaggtacat ccaataatat gtaaaagttc gtttcgtgca tttagacctt tggaacgctt
      661 taaggaactt aggaaaaaaa taagggaaat acagcctgat ctggttattt cgttccttgc
      721 tgatgtaaat atccatgtca ttatttcatt gttaggagta aaacaaaaat taattgtttc
      781 tgaacgaaat gatccacaac ggaatccaga agaaccatgg attaggaaaa ttagagattt
      841 tctttataaa aatgtagatg gaattgtttt tcaaacacca gacgcaaaaa aatattttag
      901 taagatcaat gata
//
LOCUS       DAYYAR010000299          905 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000299 DAYYAR010000000
VERSION     DAYYAR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.306"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..905
                     /locus_tag="ACHAT0_002992"
                     /pseudo
     CDS             1..905
                     /locus_tag="ACHAT0_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          330 a          157 c          172 g          246 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacac
//
LOCUS       DAYYAR010000300          897 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000300 DAYYAR010000000
VERSION     DAYYAR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.307"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACHAT0_002993"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACHAT0_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8452846.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          256 a          188 c          138 g          315 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagatt
//
LOCUS       DAYYAR010000301          874 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000301 DAYYAR010000000
VERSION     DAYYAR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.308"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>874
                     /locus_tag="ACHAT0_002994"
     CDS             <1..>874
                     /locus_tag="ACHAT0_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8452847.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRK"
BASE COUNT          380 a          114 c          142 g          238 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaac
//
LOCUS       DAYYAR010000302          849 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000302 DAYYAR010000000
VERSION     DAYYAR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.310"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..799
                     /locus_tag="ACHAT0_002995"
     CDS             107..799
                     /locus_tag="ACHAT0_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8452848.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          374 a          131 c          146 g          198 t
ORIGIN      
        1 atagtattta tattaaaatt acatatgtaa tttaaaaacg cttgacattg tgtttgagat
       61 cacttacttt tagcatgtaa aagtattgga ttaaggagct atgcaaatga aaaagaaatt
      121 tattactggt gggattgtcg ttgcatttct atgtctaatt ggctacacag gcacgcggta
      181 cgcaaatgaa attattttgt ggggtggaga gacagatatt gatacaatca atgagaatct
      241 gacacagctt gatcatacat tgacagataa agagcaagcg attacagatc ttaccgctcg
      301 actagcacaa aaagaacaga cattgcaaga ttcaaaggca gatgtccaag aataccaaac
      361 aaaagtaaat gaattagaaa accaaaaaaa tcaatcggca gcagaaaaac aaaacttaga
      421 aagtcaatta gtcagcaagg atgcacaact acaaagcaaa caaaatgaaa tcaatgtcaa
      481 actagatgag atcaaccaaa aaaatgatga aatcaatcat ctgaatgcag attggacagc
      541 aagactacaa gaagcacaag caaaactttc agaattacaa aatactgtaa atggtttgaa
      601 tcaacaaatc gcaacactga cacaagaaaa aacaaatgta gaaactcagt taacagatac
      661 acaaaagaaa ttagacgaag caactgctga aaactcaagc ttgaaacaat atattcaaaa
      721 gttagaaaaa gcaaaagatg aagtggaaga tacagctgat aaatctcagc aaattgttga
      781 agaacatttg cgtaaataat ctttcttaac aagtaaacct aaattagtaa agaagcaaca
      841 gtaatctat
//
LOCUS       DAYYAR010000303          829 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000303 DAYYAR010000000
VERSION     DAYYAR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.311"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>829
                     /locus_tag="ACHAT0_002996"
     CDS             <1..>829
                     /locus_tag="ACHAT0_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452849.1"
                     /translation="YMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHIN
                     RALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVT
                     LITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILL
                     GFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLII
                     KLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELL
                     "
BASE COUNT          264 a          110 c          121 g          334 t
ORIGIN      
        1 ggtatatgaa tttgaaaaaa attttttctg taaaaatatt tatagtttat gcatttgtta
       61 gtttattata tttatttcca gcatttaaaa acatggaaat atttggcttt tatgatacgg
      121 catttcacat taatcgtgcg ttaagtttag aatctatttt tagctcccct attaattttg
      181 aaacgtttag atcatatggc atgcaagtaa ataattttta cccatggtta actctatatc
      241 ctttattttt attgatcaag tttactaatt tagcaatagg ctataacctt tttctttata
      301 ttgttacgct cattactcta tttatatgtc attatgtaat gtatgaaatt accaaaaaac
      361 atgttactag ctcctttttc gctattatat atacaacatc ttcttttagg agtgtagaaa
      421 tatttcttcg aggggcaatg ggggaattac tcgcaatgtc aatacttcct cttattttac
      481 tcgggtttat aaagctttac gactcaaaaa aagaatcttg ggttatgttg gcgattagca
      541 tgactttatt aatttacact catgttcttt ctgtagctat gactatggta atgattataa
      601 ttgctttaat tattcaattt tatcggaaaa agaaaataga aatcttctta attattaaat
      661 taataaaagc tgctatagtt actttacttc tttcactagc gtttataggt cctatgattg
      721 agcagacatt gtatcaagat ctgaatagac cttatgtgga tatattacaa aaaagagcaa
      781 tttatctggg taaagagttt ttgattggaa gtatagattc agaattact
//
LOCUS       DAYYAR010000304          826 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000304 DAYYAR010000000
VERSION     DAYYAR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.312"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHAT0_002997"
     CDS             136..309
                     /locus_tag="ACHAT0_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452850.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          277 a          135 c          104 g          310 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actcca
//
LOCUS       DAYYAR010000305          791 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000305 DAYYAR010000000
VERSION     DAYYAR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.313"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..716
                     /locus_tag="ACHAT0_002998"
     CDS             <1..716
                     /locus_tag="ACHAT0_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8452851.1"
                     /translation="KKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTST
                     ESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADK
                     SAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWS
                     ESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNE
                     TANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DAYYAR010000306          787 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000306 DAYYAR010000000
VERSION     DAYYAR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.314"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..>787)
                     /locus_tag="ACHAT0_002999"
     CDS             complement(42..>787)
                     /locus_tag="ACHAT0_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8452852.1"
                     /translation="RNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSLKHVQKL
                     MKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAADITYIPTKKNGWC
                     YLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTARE
                     VEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIE
                     GFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          214 a          126 c          136 g          311 t
ORIGIN      
        1 ggatttgagt caatatcttg gacacatttt agtagagacg gttaagccat tttttctttt
       61 gctaattctt caaattcctg tggggttaga tagtgaatcg agctatggat tcgatttcgg
      121 ttataaaatc cttcaatgta gctaaatagc gctcgatttg cttcttcaaa atctgagtag
      181 ctagtcgtgt agacttcttc ttttttcaat gaggcatgga aagattcgat tccagcatta
      241 tcataaggtg ttccttttct actataagaa tgccttattt tgttggtttt aagccattgt
      301 tccacttctc ttgctgtgta ttggctgccc aagtcagtgt gtagaatcat tccttctgga
      361 atgtgataat gtatttttgc tttattcaat gtttgaatga cacaatccac agtcattcgt
      421 tttgaaaatg tataactaat aatttttttc gtgtgtaaat ccatgattga agataagtaa
      481 caccaaccat ttttcttagt aggaatgtac gtaatgtcag ctgcccattt ctcacagatc
      541 gtttcagtag aaaagtcctg atttaagaga ttctcttttg aaatgatcgg tttattagac
      601 ctttgaggtc tatatttttt aactacaatc gaccttaaat ttaattgttt cattagcttt
      661 tggacatgtt taagagatac tgaaatccct tcttttaata aaacttgatg gatctttgtg
      721 gcaccatagc gttgtttatt cgtaaaaaaa attgttgaaa tcttcttttt caacttatta
      781 tttctga
//
LOCUS       DAYYAR010000307          783 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000307 DAYYAR010000000
VERSION     DAYYAR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.315"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..263)
                     /locus_tag="ACHAT0_003000"
     CDS             complement(<1..263)
                     /locus_tag="ACHAT0_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFM8452853.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINR"
     gene            complement(256..>783)
                     /locus_tag="ACHAT0_003001"
     CDS             complement(256..>783)
                     /locus_tag="ACHAT0_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8452854.1"
                     /translation="PRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQW
                     WALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLP
                     LELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLL
                     NRIDKEILKVGNIDE"
BASE COUNT          273 a          125 c           92 g          293 t
ORIGIN      
        1 tctctgttaa tgattatagt taagataaaa ctaatcattg ctccaatcag tagccaagaa
       61 aaacgcattt taaaaaaatt tttctttata aaaacctcat acaaaactat aattccaccc
      121 caagcaaaag ctattttcat cgggatatta gttacattaa aagtactagg cataaaataa
      181 agcagacaaa tgattaaatt tattactcga aacgcatctc gacttaataa actcttctct
      241 aaaacaggta ctattctatt catctatatt tccaactttc aaaatttctt tatcaattct
      301 atttaatagt ttatcagagt aatccgattt aacttttctt acaaataatt tttcactttc
      361 ttttatttga tctatatccc tttcatcaaa ccatttgttc atggaaggat caataatatg
      421 aatatttgct aatttcgaag tttgtaaagt agtttcttca atatttttca attcaagtgg
      481 taaatctggt cctccaataa catttttatt tttgaacgag gaattaaaaa aaagtgtatg
      541 aaaaaatttt tcatctgggg caaacatacc tattttaaaa ttcaggtagt cttgttgatt
      601 ttgttcatac atttgtatta attctgttag gcattctttc gacaaagccc accattgaga
      661 accatgatac aaatcaaatt tatggtgtct aatttttggg ggaagtttaa tcatcctcag
      721 acaacgatta attatagctc taaaaatttt aaatgcagtt gtgttattcc tattaattct
      781 agg
//
LOCUS       DAYYAR010000308          771 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000308 DAYYAR010000000
VERSION     DAYYAR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.317"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..482
                     /gene="ssrA"
                     /locus_tag="ACHAT0_003002"
     tmRNA           116..482
                     /gene="ssrA"
                     /locus_tag="ACHAT0_003002"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          251 a          126 c          150 g          244 t
ORIGIN      
        1 aaaggggcag ttattatata aataatggga tatgattcgc ttttttattg aatttatccc
       61 aatatatgct atactaattt tgtatgaaaa atacacgtaa cccttgttag ctaacgggga
      121 cgttacggat tcgacaggca cagtcgagct tgaattgcgt ttcgtaggtt acgtctacgt
      181 aaaaacgtta cagttaaata taactgctaa aaacgaaaac aactcttacg ctttagctgc
      241 ctaaaaacag ttagcgtaga tcctctcggc atcgcccatg tgctcgagta agggtcctaa
      301 ctttagtggg atacgtttca actttccgtc tgtaagttga aaaagagaac atcagactag
      361 cgatacagaa tgcctgtcac tcggcaagct gtaaagtgaa tccttaaatg agttgactat
      421 gaacgtagat ttttaagtgg cgatgtgttt ggacgcgggt tcgactcccg ccgtctccat
      481 atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt
      541 attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc
      601 aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg
      661 tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa
      721 ggaaaaacac aattatggtt taagcatgtt ccttcaaata agttttttta a
//
LOCUS       DAYYAR010000309          770 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000309 DAYYAR010000000
VERSION     DAYYAR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.318"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..>770)
                     /locus_tag="ACHAT0_003003"
     CDS             complement(86..>770)
                     /locus_tag="ACHAT0_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452855.1"
                     /translation="FVLLLHGRVINLVNRKSTTKFANSFWFLENINETVEKNGVYKGD
                     FHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSN
                     LKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSY
                     YWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGTLGWTETYYEKIYK
                     EIIEQYFPK"
BASE COUNT          248 a          138 c           75 g          309 t
ORIGIN      
        1 ccactcaaaa tcttgacaaa agaaaaaagt tgtgatttcc aaaatcacaa cttttttctt
       61 ttacaataaa tcaatcctta ttaaattatt taggaaaata ttgttcaatt atttctttat
      121 atattttctc atagtatgtt tctgtccatc ctaatgttcc tataaaatca agtcctctac
      181 attttaaagg ttcacttaaa ctattaatat agttaataat gttgtctatc cttttctctt
      241 tatcttcacc ttcaatctgg ggagcatata gtccaatttc ctcccaataa tatgaatctc
      301 tttcaattcc catgatggta gctttggcta atttcaaatc aaataacgat tttagaattc
      361 ttagtgtctc aataaagtct tctctcttca actctttaat ccattgtgaa ttttctggat
      421 tatctaaaat ttcaagttca tcttcattat aagtatattt caaatttgac ttgcttaact
      481 catagataaa tgaagtttct tgactcataa ttcttttaag tacttgacta taagtttcgt
      541 ttgactttgc ccatttcgct aattctacca ctgtattcac taataaatcg aaataatgat
      601 gttctcgttc aatagtctca gcattttgaa aatgaaaatc acctttataa actccattct
      661 tttccactgt ttcattaata ttttctaaaa accagaatga gtttgcaaat ttagtcgtac
      721 tttttcgatt aactaagtta ataactcttc catgtaataa taagacaaaa
//
LOCUS       DAYYAR010000310          767 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000310 DAYYAR010000000
VERSION     DAYYAR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.319"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>767
                     /locus_tag="ACHAT0_003004"
     CDS             <1..>767
                     /locus_tag="ACHAT0_003004"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8452856.1"
                     /translation="IICVKKDGTRFSQEYDKLILATGSKQIMLDVPGNNLANIFSIKN
                     FPNAKEIFKALDDNTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDR
                     SFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNS
                     PIGKKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTA
                     YIATQHIDNQDFTPSGSQFTNGFSIFNYNFYAAGLNL"
BASE COUNT          287 a          100 c          132 g          248 t
ORIGIN      
        1 attatttgtg taaaaaaaga tggcacaaga ttttcccaag aatatgacaa acttatacta
       61 gctacaggtt caaagcaaat tatgttagat gtaccgggga ataatttagc taatattttt
      121 tcgataaaga attttccaaa tgctaaagag atttttaaag ctttagatga taatactatt
      181 aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga agccataaga
      241 aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc gacttattac
      301 gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat acatctttgt
      361 ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat agtcactaca
      421 agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct atctaatagt
      481 ccaattggca aaaaggaact cttgagagat gttagtggag catatctaac taatgaatac
      541 cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac tttttttacg
      601 tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac agcttatatt
      661 gcaactcaac atattgacaa tcaagatttt acaccttctg gttcccaatt tacaaatggc
      721 ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaa
//
LOCUS       DAYYAR010000311          766 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000311 DAYYAR010000000
VERSION     DAYYAR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.320"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..722
                     /locus_tag="ACHAT0_003005"
     CDS             <1..722
                     /locus_tag="ACHAT0_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8452857.1"
                     /translation="KQQINDLYSTLLRTIDDLEFKNKEILKLEKLKYDFFKGASHELK
                     TPLASLKIILENMKYNIGKYKERDIYINECIEIVDSLTKNISQILSVHSIENLNNDEE
                     YLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTALKIILSNLISNAVKYTD
                     VNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNY
                     NIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          341 a           53 c          105 g          267 t
ORIGIN      
        1 taaaacaaca aattaatgat ttgtattcta ctttattaag aacaattgac gatttggaat
       61 ttaaaaataa agaaatttta aaattagaaa agttaaaata tgactttttt aaaggtgcat
      121 cccatgaact taaaacccct cttgctagtc ttaaaataat actggaaaac atgaaataca
      181 atattggaaa atataaagaa agagatattt atattaatga atgtattgaa atcgttgata
      241 gtttaacaaa aaatatttct caaatattat cagttcattc tatagaaaat ttgaataatg
      301 atgaagaata tttgaaaata aatgatacat tagaagacat attaaaaaag tatgaaatat
      361 tagcaaatca aaagaaaata actgtcaata attatatatt agacgagaat gtttatatag
      421 gaaaaacagc tttaaagatt attctatcta atttgattag taatgcggta aaatatactg
      481 atgtaaatgg ggtaattaat attgggatag ttaatgattg gttatatata gagaattcat
      541 acggtaacaa taaaatttcg aatatggata aaatatttga tgttaaattt gatttaaata
      601 aggaaaatag taatggatta ggtttatata ttgtaagtaa tattctgaat aattacaata
      661 tagaatataa agtattgcag gatgaagaat tttttatctt taaaataaaa atattttctt
      721 aattaagata taattttgca atagttattg aaaaatagag acttat
//
LOCUS       DAYYAR010000312          764 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000312 DAYYAR010000000
VERSION     DAYYAR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.321"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(237..>764)
                     /locus_tag="ACHAT0_003006"
     CDS             complement(237..>764)
                     /locus_tag="ACHAT0_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452858.1"
                     /translation="GLIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLV
                     ELDVNNYRELYVFNSLDVEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKNIKKCDK
                     CFESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIK
                     KAFKIKTIGTAPNFK"
BASE COUNT          261 a          103 c           85 g          315 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttatattt
      481 ttacctttgt aaaaatattc gattgaatat tctatttggc acgactcact tggcagtata
      541 tgtatgtcaa tatttttcat ctgtttcata ggtatttcaa catctaaact attaaaaaca
      601 tataattccc tgtaattatt tacatctaac tctactaaat ttatctttgg aacaatagat
      661 cctccaccca ttccaccata ataaaaaaaa tcttgttctc tatcaaaata gcacatatta
      721 agctcagttt gtttatcatc ctcaataatt ttataaatca aacc
//
LOCUS       DAYYAR010000313          739 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000313 DAYYAR010000000
VERSION     DAYYAR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.322"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..>739)
                     /locus_tag="ACHAT0_003007"
     CDS             complement(7..>739)
                     /locus_tag="ACHAT0_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8452859.1"
                     /translation="ICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          282 a          130 c           85 g          242 t
ORIGIN      
        1 ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg cttcccaaat aataccagaa
       61 aacaattgaa atgacatcgc gcttattgaa aagtacatga gcctccacca tttcatctat
      121 aaaaatagat agtataaaaa ttaaaacaat accaagaaac gcataattca ctttacttct
      181 aaaaaatgtt ttgagtaaac taaagatatt taacacatag aaagcataga tacatataaa
      241 agcaattata ccacttgata caagtacttg aagaaatcca ttatgcatgt ttccatgtgc
      301 tctttttagt tcattcacat ctgtagtatc aagatctact tttttcaaaa attttgcatc
      361 gctattccca tagaaatcgg tctctccata accaaataac aaagttggtc ttataacttt
      421 aagcgcagct ttccaaatcg taaaacgacc agctgaaatt tcggagttat cactagaatg
      481 ctgtaatatt acttcttttt ctggaagtgg agttttatct gattcatttg actctataac
      541 ttctactttt ctatcaatcg atacaggaat tgttgctaaa aaatccttag cataattagt
      601 accaaaagaa aaatatacca atagaactaa taatatgctt accatccatt tgatttttac
      661 aggtagtact tctctaatgc tctttctaaa ttttgaattg gcaaacaaca aaagaaataa
      721 tactaaaaaa gaacaaatt
//
LOCUS       DAYYAR010000314          735 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000314 DAYYAR010000000
VERSION     DAYYAR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.323"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>735)
                     /locus_tag="ACHAT0_003008"
     CDS             complement(<1..>735)
                     /locus_tag="ACHAT0_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8452860.1"
                     /translation="FGPFRANNIDKDFFKLKMNNLNVAMADRERLYNSLQNLTLYSLG
                     DIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKTIEQIKIDNLLSQNYDPEIYLFLY
                     ENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLG
                     MDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQ
                     FIGLNIYKRITVKEIANYFSVSETKL"
BASE COUNT          250 a          121 c           86 g          278 t
ORIGIN      
        1 ttaatttagt ttcacttaca gaaaaataat tagctatttc ctttacagta attcttttat
       61 agatatttaa gccgatgaat tgaacgacag aagaaattaa gggagaaaga tgctctatct
      121 tagtttttcc gatttcctta gtataataaa caatcccaga atccctcact tttaagacct
      181 caggtaacga tacagctaat tcgttttctt gtatcaaagc atctctactt tgatacgctt
      241 ctattatatc cattcctaat gttatagacg tttgtgctaa cttttcaaaa acaatgatag
      301 aataattttt ttcagaccga atcgtatctc cactaacact tggaattatt ccattactca
      361 gattaaaaac catgttttct aaatttcgaa tatctccatt tttaacatat tccagtattt
      421 tattttcata aagaaacaga tatatttctg gatcgtaatt ttgtgataac agattatcta
      481 ttttgatttg ctcgatagtt ttacttagat ttcctgtata ttcaattaac ggttcatgga
      541 atagatcttc tatttttcca gtaaaaaaat aatgaactaa aatcagaata tctcgaatgt
      601 ctcctaaaga atacaaagtc aaattttgca atgaattata gagtcgttct ctatcagcca
      661 ttgcaacatt caaattgttc atttttagtt taaaaaaatc tttatcaata ttatttgctc
      721 taaatggacc aaata
//
LOCUS       DAYYAR010000315          715 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000315 DAYYAR010000000
VERSION     DAYYAR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.325"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..294
                     /locus_tag="ACHAT0_003009"
     CDS             58..294
                     /locus_tag="ACHAT0_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8452861.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            291..689
                     /locus_tag="ACHAT0_003010"
     CDS             291..689
                     /locus_tag="ACHAT0_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8452862.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT          258 a          113 c          128 g          216 t
ORIGIN      
        1 atacttggat ataatgtact tactatagaa tgagaatggt atagagggtg gcgaaatatg
       61 aaaaaaatga aaaagtataa aattagaaaa tctggcaata gcgatgtaac aactatccca
      121 ccagaagtaa aagaattcat gggtgtgcag acaggtgacg caatcagtta tgtttttcaa
      181 tctgatggtt ctgtacgaat gatcaaagca caagaagaac ctgatattga ttcattagtt
      241 gattcgatca tgaatcaata tgaagacgcg ttaaaagatt tggttgattt atgaggtatc
      301 tttcgactaa agaaattatc aaaatcaacg cgaaagttat tttacgtcac tcgccaggcg
      361 aaatgatcgg tgtaaaagat gcaaatgcat tagatatggc cgtcaaacag ccttcacaag
      421 cagtattcaa tcaagaactc tatcctgaaa tctatgaaaa agcggcgatt ttagcaatta
      481 atttagctaa aaaacatcct tttcataatg gcaataaacg aactgctctt gttgccatgt
      541 tgttgtttct tcaattgaat aattgtccag ttgctttttc tcgtcaagaa gctgttgatt
      601 ttatcataat gattaccact agttcattgg atttcgattc actaaagtcc aagataacca
      661 gttatttacg acaaaccgca ataaaatgaa cgaattaaaa actttatttc tcaat
//
LOCUS       DAYYAR010000316          710 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000316 DAYYAR010000000
VERSION     DAYYAR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.326"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..166
                     /locus_tag="ACHAT0_003011"
     CDS             <1..166
                     /locus_tag="ACHAT0_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8452863.1"
                     /translation="QISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDY
                     REKLRLLRSL"
     gene            270..>710
                     /locus_tag="ACHAT0_003012"
     CDS             270..>710
                     /locus_tag="ACHAT0_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8452864.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKY"
BASE COUNT          248 a          101 c          150 g          211 t
ORIGIN      
        1 tcagataagt gatagtacac tgaaccgttt agtaaaactt ggtgagtttc ctccctgttg
       61 gtacgggatt cgaggacact attttcgtga tgatgtttta gaatggatga gaaagagtga
      121 ttcgaatgac tatcgagaaa aattgcgact acttcgatct ttataagtta cattttcttt
      181 ctaagatgcg gtacaatgat gacatagata gaaagatttc caaagggttt ctgcttgttg
      241 ggttccttca aaataggagg aatcaataaa tggaaaaaac agatcgattc aagaagcaaa
      301 tcagttctac tggtaggatc acatggtact ttcaagcata tcgaacaaca agacgtggct
      361 ttaattcaaa acgtgaggcg caactcgctt atctggaaat ggagaaacaa caaaggctta
      421 agaaaaaaat tgttgcaagc aatgacaagt ttagtgttgt aggtgagcaa tggttccaat
      481 attaccgttc gttaaatgaa caaaaggaaa taacctatga taagcgagag gaacaattaa
      541 aaattattgt tcgatggatt ggtgataaaa agttgtctga tttgacacca gagtgccttc
      601 aagaattgct ttttacctta aaggaatgtg gcgttaatgg aacaaatgtt ggctatgcca
      661 aaaatagttt acaatcactt attcaagttc taaatatgat ttttaaatat
//
LOCUS       DAYYAR010000317          619 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000317 DAYYAR010000000
VERSION     DAYYAR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.327"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..436)
                     /locus_tag="ACHAT0_003013"
     CDS             complement(<1..436)
                     /locus_tag="ACHAT0_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452865.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT          183 a          112 c           86 g          238 t
ORIGIN      
        1 tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat actttataag
       61 ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata tatttttcct
      121 gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga gataacagag
      181 ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg acggttactc
      241 gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg atcgtttttt
      301 tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac tctgataaat
      361 ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct ttgcttaaaa
      421 aaaactcata attcattagt catcagctct ttctacgtat attactttcc atgcattcac
      481 caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca gcgattcttg
      541 tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt tttatttggc
      601 ctttctcgcg atcaaacct
//
LOCUS       DAYYAR010000318          593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000318 DAYYAR010000000
VERSION     DAYYAR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.328"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>593)
                     /locus_tag="ACHAT0_003014"
     CDS             complement(<1..>593)
                     /locus_tag="ACHAT0_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8452866.1"
                     /translation="LLRMFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFT
                     DVSAAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKG
                     YGYVVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAH
                     QYQVFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQ"
BASE COUNT          264 a           87 c           65 g          177 t
ORIGIN      
        1 tattgttctc gttgcctaat agttattcta ataaataaaa atagaacaac aaacatttga
       61 atgattaaaa atattaagac accaaacaca aaatgatcaa atacttgata ttgatgagca
      121 acataagaaa caaataaacc aattgtaagt aaataaatca tgaatatagc agtaagttta
      181 gaactggact gttcaacaat cctatttata gctaattttc tatataacca aataaacatc
      241 agcaaaaaca aagaatcaag cattatctgg aatactacat aaccataccc ttttatccct
      301 tcaaatgaaa acatatacgt cataaccatg gaagtaataa tagatctgaa atcatcaata
      361 agcatacata atagtaattt atttaaaagc actatattag ttttcttctt ttcctttaat
      421 ataaaataac ttccgattat aggtatagct gcggaaacat cagtaaataa ttcaacaaaa
      481 aaagtgagtg gtatcaataa acatacaaaa agatattttc ttttgttaag atcagtatta
      541 gtcatataaa ataagagaaa aaagaaaaag tttcccacga acattcttaa tag
//
LOCUS       DAYYAR010000319          583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000319 DAYYAR010000000
VERSION     DAYYAR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.329"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..>583)
                     /locus_tag="ACHAT0_003015"
     CDS             complement(38..>583)
                     /locus_tag="ACHAT0_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8452867.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          167 a          110 c          100 g          206 t
ORIGIN      
        1 aaatccactc cttttttcat gctctaaaat gatattttta cacaactgtt acggttgttt
       61 tacgaaattg gacgggactt tgccagttta atttctgttt tatccgttca ttattgtagt
      121 aatagatata gctatcgatg gctgatttta attcctcgaa acagttataa actttcccgt
      181 gaaaaatctc ttgttttaat aagccaaaaa aattctccat aagtgaatta tctaagcagt
      241 tccctttccg agacatgctt tgaaagattt tgtactcctt caatttgttt ctataagctt
      301 tcatttgata cgcccaacct tgatctgagt gaaacgtacg acgataggga caatcgttag
      361 tggtctggat agcttcctcc aaaccttcca ttaccgctaa tgcattgggc ttttcagaga
      421 tacgatagga taaaatctca ctattgaaca tatccataaa aggatctaga tacaatttct
      481 tttgacggat aatgcctttg gcatccactt catggtattt aaattcagtt gtatccgtcg
      541 tgattttctg atggcagata ctggtgtaga aacgacgatg gat
//
LOCUS       DAYYAR010000320          575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000320 DAYYAR010000000
VERSION     DAYYAR010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.330"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHAT0_003016"
     CDS             complement(132..509)
                     /locus_tag="ACHAT0_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452868.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          174 a          103 c          101 g          197 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagta
//
LOCUS       DAYYAR010000321          575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000321 DAYYAR010000000
VERSION     DAYYAR010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.331"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..575
                     /locus_tag="ACHAT0_003017"
                     /pseudo
     CDS             258..575
                     /locus_tag="ACHAT0_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          195 a          105 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DAYYAR010000322          575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000322 DAYYAR010000000
VERSION     DAYYAR010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.332"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          186 a           81 c           81 g          227 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct tttta
//
LOCUS       DAYYAR010000323          574 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000323 DAYYAR010000000
VERSION     DAYYAR010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.333"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>574
                     /locus_tag="ACHAT0_003018"
     CDS             <1..>574
                     /locus_tag="ACHAT0_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8452869.1"
                     /translation="TYSIDTHLHLSAAPYFRLLINESANLYAEFAHKSIELDNPSLLF
                     IDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEITSIITFLKNEQFK
                     IDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRHYQQELAL
                     LTIPSNEKVIPKKKHILYSDELVDPIIRYLE"
BASE COUNT          200 a          108 c           64 g          202 t
ORIGIN      
        1 acctattcca tagataccca tcttcattta tctgctgctc cctattttcg actactaata
       61 aatgaaagcg ctaatttata tgctgaattt gcacataaat ctatagaact agataatcct
      121 tcccttttat tcattgataa acagcaagaa gtccaaatct tttctactca aaaggaaaca
      181 caaactttta tcattatcca gttttatttt gatgagcaat tattagcaca tattacggga
      241 caatcttttt ctatagatca agaaataaca tctatcatta cttttctgaa aaatgaacag
      301 ttcaaaattg attatctaaa acatagtaag ccagctattt cctatgatca ggaaatcaat
      361 tcttttttca tactatgcca acagatgagc attcatttgc tttccttatt agttacttat
      421 tgtctgcaaa aaattcaatt gtcttcctta ccaaggcatt atcaacagga acttgcttta
      481 ttgacaatac cttcaaatga gaaagtcatt cctaaaaaaa agcatattct ttattcagat
      541 gaattagttg atccaatcat acgatattta gaag
//
LOCUS       DAYYAR010000324          569 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000324 DAYYAR010000000
VERSION     DAYYAR010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.334"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>569
                     /locus_tag="ACHAT0_003019"
     rRNA            <1..>569
                     /locus_tag="ACHAT0_003019"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          139 a          131 c          173 g          126 t
ORIGIN      
        1 gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccagtggc
       61 gaaggcggct ctctggtctg taactgacgc tgaggctcga aagcgtgggg agcaaacagg
      121 attagatacc ctggtagtcc acgccgtaaa cgatgagtgc taagtgttgg agggtttccg
      181 cccttcagtg ctgcagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt
      241 gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa
      301 gcaacgcgaa gaaccttacc aggtcttgac atcctttgac cactctagag atagagcttc
      361 cccttcgggg gcaaagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt
      421 tgggttaagt cccgcaacga gcgcaaccct tattgttagt tgccatcatt cagttgggca
      481 ctctagcaag actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg
      541 ccccttatga cctgggctac acacgtgct
//
LOCUS       DAYYAR010000325          560 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000325 DAYYAR010000000
VERSION     DAYYAR010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.336"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..535
                     /gene="spxA"
                     /locus_tag="ACHAT0_003020"
     CDS             137..535
                     /gene="spxA"
                     /locus_tag="ACHAT0_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8452870.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          196 a           87 c          110 g          167 t
ORIGIN      
        1 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
       61 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
      121 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
      181 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
      241 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
      301 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
      361 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
      421 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
      481 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
      541 taatacgaaa cttggaaaag
//
LOCUS       DAYYAR010000326          547 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000326 DAYYAR010000000
VERSION     DAYYAR010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.337"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACHAT0_003021"
     CDS             168..362
                     /locus_tag="ACHAT0_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452871.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          233 a           68 c           88 g          158 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaa
//
LOCUS       DAYYAR010000327          538 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000327 DAYYAR010000000
VERSION     DAYYAR010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.338"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..>538
                     /locus_tag="ACHAT0_003022"
     CDS             35..>538
                     /locus_tag="ACHAT0_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8452872.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNV"
BASE COUNT          207 a           68 c          108 g          155 t
ORIGIN      
        1 tatagatgac tataattcat aggaggagat ataagtgttt aaaggaaaaa caatattagt
       61 aactggcgga actggttcca ttggaagtga aattgttcgt cagcttttaa aatatgaacc
      121 aaaagctatc agaatttata gtagaggtga agaaaaacaa ttttatatgc aacaagagct
      181 ctcaaatcac actaatttga gatttttaat tggtgatatc agagactctc aaaggattga
      241 ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc gcaatgaaac acgttccagc
      301 aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg atgggaactc aaaatgtaat
      361 agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt atcagtacag ataaagtagt
      421 gagtcctact aacaccatgg gtgcgacaaa gttattgagt gaaaaattaa taatagctgc
      481 caatcagtat aaaggaaaaa cagaaacaat attttcttgt gtaaggtttg gaaatgtc
//
LOCUS       DAYYAR010000328          521 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000328 DAYYAR010000000
VERSION     DAYYAR010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.339"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>521)
                     /locus_tag="ACHAT0_003023"
     CDS             complement(<1..>521)
                     /locus_tag="ACHAT0_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8452873.1"
                     /translation="CYNVEKYIDECINSLINQTLKDIEIILLNDGSTDNTLAILKKYE
                     KLDSRIKVYEHKNMGLGPTRNRGIKIANGEFLAFVDSDDFVSNTMFESLYNCAIAENA
                     DIAIGEVGIFSDREIERVKIRKSLTNLKTIELATYNKKEFFKQFYFNRLYSHNAWDKI
                     YRRSLVLEFNLSFG"
BASE COUNT          174 a           99 c           45 g          203 t
ORIGIN      
        1 ccaaatgata aattaaactc taatactaaa ctacgtctat atatcttatc ccaagcatta
       61 tgtgaatata aacgattgaa atagaattgc ttaaaaaatt ctttcttgtt atatgttgct
      121 aattcaattg tttttaagtt cgttagactt tttcttattt ttactctttc tatttctcta
      181 tcagaaaaaa tgcctacttc tccaattgca atgtccgcat tctctgcaat tgcacaatta
      241 tataaacttt caaacatagt attggaaaca aaatcatcag aatccacaaa agccaaaaat
      301 tctccattag ctattttgat ccctcgattt ctagttggcc ctaaccccat atttttatgt
      361 tcgtaaactt ttattctaga atcaagcttt tcatatttct ttaatatcgc caacgtatta
      421 tctgttgaac catcgtttaa caaaataatc tcaatatctt tcaatgtttg atttatcaaa
      481 ctatttatac actcatcaat atatttttca acattataac a
//
LOCUS       DAYYAR010000329          514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000329 DAYYAR010000000
VERSION     DAYYAR010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.340"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHAT0_003024"
     CDS             complement(<1..132)
                     /locus_tag="ACHAT0_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8452874.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAYYAR010000330          497 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000330 DAYYAR010000000
VERSION     DAYYAR010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.341"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>497
                     /locus_tag="ACHAT0_003025"
     CDS             <1..>497
                     /locus_tag="ACHAT0_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452875.1"
                     /translation="PLIPGYYTLYVKIGETIFYSRFEIILKDLGTPEWEKMKIEIEKM
                     VGGLARTFIPKKTSRREITTSNYLMNDQLFQRILEFENNIPQAIVSLESLKKEAKYRI
                     DKRYNWKPIGSKNLVDQKTISQLQIHPEKRGLLYTPSRIIKYDIIENQWIKFILSYFK
                     NFSLLA"
BASE COUNT          196 a           77 c           67 g          157 t
ORIGIN      
        1 cctcttattc caggttatta cactctttac gttaaaatag gtgaaactat cttttattct
       61 cgatttgaaa taatacttaa agatttaggt acaccagaat gggaaaaaat gaaaatcgaa
      121 attgaaaaaa tggttggcgg tcttgctaga acttttattc ctaaaaaaac ttctcgtcgt
      181 gaaattacta cttcaaatta tctaatgaac gatcaacttt ttcaacgaat attagagttt
      241 gagaacaata tacctcaagc aatagtttct cttgaaagtt taaaaaaaga agcaaaatat
      301 agaattgaca aacgatataa ctggaaacca attggaagca aaaatttagt ggatcaaaaa
      361 actatttcac agttgcaaat ccatccagaa aaacgtgggc tactatatac acctagccgc
      421 ataataaaat atgatattat tgaaaatcaa tggatcaaat ttatcttatc ttattttaaa
      481 aatttttcac ttctagc
//
LOCUS       DAYYAR010000331          492 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000331 DAYYAR010000000
VERSION     DAYYAR010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.342"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>492
                     /locus_tag="ACHAT0_003026"
     CDS             <1..>492
                     /locus_tag="ACHAT0_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8452876.1"
                     /translation="AKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLP
                     DWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSD
                     SFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMT
                     DIQL"
BASE COUNT          179 a           61 c           86 g          166 t
ORIGIN      
        1 gctaaaaaga aagatagatt actttatcta tcttctacat ttttgaagag aaaagaaatt
       61 caatatatta agtctaaagt gacgcataat aaaaaaattt cttatcaata ccatgtagga
      121 cagtatttac ccgactggca tccttgggaa gattatcgca atttttttgt ttcaaacaaa
      181 aaaactaatg ggtgtagaga aatattcgct attgaattac cttggattat agatacattt
      241 gggaaagtta aatcgtttac tgtaatgacc cagaaattat ccaatcttga tattgattat
      301 tcggacagtt tcactcttat tattgagcat gaaactggta tagttggaac tttaagtgta
      361 gatgttgttt caagagtagc taaaagaaat ttaactataa ttggtgaaaa gattttaata
      421 gaatgggaag gtaacccgga ttctctattt tcatggaatg aagaaacttc tagtatgaca
      481 gatattcaat tg
//
LOCUS       DAYYAR010000332          476 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000332 DAYYAR010000000
VERSION     DAYYAR010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.343"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..>476
                     /locus_tag="ACHAT0_003027"
     CDS             125..>476
                     /locus_tag="ACHAT0_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8452877.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIF"
BASE COUNT          180 a           78 c           86 g          132 t
ORIGIN      
        1 ccgcactatg ccattacatt tatatctacg cgaattgtaa aattaagtta gaaaaataaa
       61 aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc
      121 ataaatgacc tacaaacatc ttaccataga cgaactgaca atgatagaat catattatct
      181 tcaacataat aagccggttg aaatcgctaa ccgaatggga cgtgctatac aaactattta
      241 taatgtagtc aataagttca agcaaggcaa gactgctctt gattattggc accagtataa
      301 agaaaataag aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg aagtagatta
      361 cattaaagag aaagtcactc ttggttggac gcctgacgtc attatcgggc gaaaagaaag
      421 gtctgtttca tgcggtatga gaacacttta tcgtttattt tctaaaggaa tatttg
//
LOCUS       DAYYAR010000333          462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000333 DAYYAR010000000
VERSION     DAYYAR010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.344"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACHAT0_003028"
     CDS             complement(<1..430)
                     /locus_tag="ACHAT0_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8452878.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAYYAR010000334          460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000334 DAYYAR010000000
VERSION     DAYYAR010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.345"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          149 a           89 c           52 g          170 t
ORIGIN      
        1 cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag atgaaagatc aggttttatt
       61 agatctcatt ctttcatcac ttgaagggat ttaatgaact actttttcct ctttgtcgtt
      121 tttatatatt cgtattcctt caaacagtag gatagcctct ttcaatgctt gatatttgaa
      181 ctgtccgtta cttaaaaatt tagatgtgcc gccctcttcc tagaacatat tcttttttac
      241 tttttcatta attgacagac caatcgaacg aaactgtaaa tttcgatagg ataatgattg
      301 gtcaaactca ttcattttca agacatctcg attcatattc aagataggaa tctttttatc
      361 aacatcactt tattcgtatt ttcctctacc ataaaagctt cacgctccac aaaattgttt
      421 tatacactac tattcagccc attcaacgac caatgaattt
//
LOCUS       DAYYAR010000335          460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000335 DAYYAR010000000
VERSION     DAYYAR010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.346"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHAT0_003029"
     tRNA            complement(20..91)
                     /locus_tag="ACHAT0_003029"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACHAT0_003030"
     tRNA            complement(113..200)
                     /locus_tag="ACHAT0_003030"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACHAT0_003031"
     tRNA            complement(225..298)
                     /locus_tag="ACHAT0_003031"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACHAT0_003032"
     tRNA            complement(312..382)
                     /locus_tag="ACHAT0_003032"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACHAT0_003033"
     tRNA            complement(384..459)
                     /locus_tag="ACHAT0_003033"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAYYAR010000336          441 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000336 DAYYAR010000000
VERSION     DAYYAR010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.347"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..180)
                     /locus_tag="ACHAT0_003034"
     CDS             complement(<1..180)
                     /locus_tag="ACHAT0_003034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452879.1"
                     /translation="MSCFVESERLSNGNNSVLNEYDKVVINKVKSLCEEARESISHIN
                     GSNFKQTLFNLIKLDA"
     gene            complement(277..>441)
                     /locus_tag="ACHAT0_003035"
     CDS             complement(277..>441)
                     /locus_tag="ACHAT0_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452880.1"
                     /translation="TAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQL
                     GLVDNFNNIS"
BASE COUNT          156 a           84 c           46 g          155 t
ORIGIN      
        1 agcatctagc ttaatcagat taaataatgt ttgcttaaaa ttgctaccat taatatgact
       61 gatagattct ctagcttctt cacataaact ttttacttta ttaattacta ctttgtcata
      121 ttcatttaac acactattat taccattact tagacgctca gattctacga aacaactcaa
      181 atcagaaatt tcccaaaaaa ttttttgtta aaatccaatt tcctcaactc ctccaaacta
      241 cgtaaatgat atagtctcat aataaaataa aacctctcag ctaatgttgt tgaaattatc
      301 cactaaacct aattgatatt gcttaggaga tacacctgcg tactttttga atactctcga
      361 aaagtgtgaa gaatctgaaa atcctaaacc taaagaaact tcactgatag tatggtttga
      421 tttcaacatt attttagccg t
//
LOCUS       DAYYAR010000337          431 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000337 DAYYAR010000000
VERSION     DAYYAR010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.349"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          140 a           78 c           69 g          144 t
ORIGIN      
        1 agcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaatttcc
       61 gtgaatttct tcttatttct tggagttaaa cgcttctgtt ccagcctctt tttatctgtc
      121 tttcactaat gatcagttgt agataagcaa gttgcttttt cttacatcat accgcccatc
      181 aagacaaacg tttttagctt gtacgtactg gacaaaaatc ccattatatc aagaaaaaac
      241 aacgttgata atacttaaaa ttattaggct agaacggact agtaagagtt ttttgcccct
      301 tttttgcccc tcaaatatcg ctttacagcc agaacataag ttcgtataat cgttttgagg
      361 tgatttttat gatggaagac tttattagaa aaaatatcag tgatgaatat gcagactttt
      421 atgagcaaag c
//
LOCUS       DAYYAR010000338          411 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000338 DAYYAR010000000
VERSION     DAYYAR010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.350"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..>411
                     /locus_tag="ACHAT0_003036"
     CDS             37..>411
                     /locus_tag="ACHAT0_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8452881.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKN"
BASE COUNT          186 a           54 c           60 g          111 t
ORIGIN      
        1 attaatagga acgtatattc gaaaggaata gaaataatgt ggttagaaaa aataaatgac
       61 aaaaaattta agtacaccga acgttatacg gatcccttaa ctgaaaaaaa gagaaaagtg
      121 tccgttactt tatctagcaa ttcacgccaa gcttggaatc aagctaactt acttttgaat
      181 gaaaaaatcg cagaaaaaat aaaacgaaat gaagaattac cactaacttt tggagaatta
      241 aaaactaaat gggatgaaaa atacaagcca actgtaaaag aaagttccta tcgaacaact
      301 caggtttatc tatcattaat ttcaaaatat ataaaagatg atgtacttgt aaaaaacgtt
      361 aatagtaatc ttatacaaga tatgctagat tactactatt ttgaaaaaaa t
//
LOCUS       DAYYAR010000339          387 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000339 DAYYAR010000000
VERSION     DAYYAR010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.351"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..376)
                     /locus_tag="ACHAT0_003037"
     CDS             complement(<1..376)
                     /locus_tag="ACHAT0_003037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452882.1"
                     /translation="MVLKQGKQDYQIIGYLEDDLNKIKINSDDVMYFFMTPLSDFPSS
                     VIDNVEGIQIYSNTLTKFGFIDDINESHSTRAQSLKEFLDPYIIVCTPKIKRNHEQAR
                     NYYQGDDLELIPKPKSEVFKNLI"
BASE COUNT          129 a           58 c           48 g          152 t
ORIGIN      
        1 ttattaaatt tttaaaaact tcagactttg gtttggggat taattctaaa tcatcacctt
       61 gatagtaatt tcttgcttgt tcatgatttc ttttaatttt tggtgtacaa acgataatat
      121 aaggatctaa aaattcttta agtgattggg cacgagtact atgagattcg tttatatcat
      181 caataaaccc aaatttagta agcgtattac tatatatttg tataccctct acattatcaa
      241 tcacactact ggggaaatct gaaagaggag tcataaaaaa gtacattaca tcatccgagt
      301 ttattttaat tttatttaaa tcatcctcca aataaccaat aatttggtaa tcttgtttac
      361 cttgttttaa aaccatttat attttcc
//
LOCUS       DAYYAR010000340          386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000340 DAYYAR010000000
VERSION     DAYYAR010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.352"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACHAT0_003038"
     CDS             <1..>386
                     /locus_tag="ACHAT0_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8452883.1"
                     /translation="VGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEYGKLLYQI
                     WKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKR
                     LVKQFDEPKVVVTDKAPSITSAFKKL"
BASE COUNT          133 a           64 c           76 g          113 t
ORIGIN      
        1 gtgggctact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat cttatatgat
       61 cgtggcatta acgtttctca tacgacgatt tatcgttggg tgcaagaata tggcaaacta
      121 ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt attcatggaa aatggatgaa
      181 acgtacatca aaattaaagg aaaatggcat tatttgtatc gagccatcga tgcagatggt
      241 ttaaccttgg atatttggtt acgtaaaaaa cgggacacac aagcagccta tgcttttctt
      301 aagcggttag tgaagcagtt tgatgaaccg aaggttgtag tcacagataa agccccctct
      361 attacaagtg cctttaagaa actaaa
//
LOCUS       DAYYAR010000341          380 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000341 DAYYAR010000000
VERSION     DAYYAR010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.353"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>380
                     /locus_tag="ACHAT0_003039"
     CDS             <1..>380
                     /locus_tag="ACHAT0_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8452884.1"
                     /translation="FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSP
                     LCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVK
                     VLETLSEEANLPNILGDKGYISHK"
BASE COUNT          133 a           71 c           62 g          114 t
ORIGIN      
        1 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
       61 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      121 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      181 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      241 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      301 cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa
      361 aggatatatt agccataaaa
//
LOCUS       DAYYAR010000342          378 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000342 DAYYAR010000000
VERSION     DAYYAR010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.354"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>378
                     /locus_tag="ACHAT0_003040"
     CDS             <1..>378
                     /locus_tag="ACHAT0_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM8452885.1"
                     /translation="RKAYGTRKLKKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFK
                     GQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGY
                     SCGEKKDASLVKEAFGRIPYSLTD"
BASE COUNT          116 a           72 c           90 g          100 t
ORIGIN      
        1 cgaaaggctt acggtacccg aaaattgaag aagtgtttag caaagcgtgg gcttcaactc
       61 agtcggcgcc gaatcggtcg aatcatgaaa cgccgcggat tgacatctac ctatacgatc
      121 gctcatttta aagggcaacg aacagcttgt aatgaagcga aaacagcgaa tgtattagat
      181 cggaccttta cacaagaaca gccattggaa gccatcgtta cggatcttac ttatgttcgc
      241 gtggggaaaa agtggcatta tatctgctta atacttgatt tgtttaatcg agaaattatt
      301 ggttattcct gtggtgagaa gaaagatgcc tcattggtaa aagaagcctt tggacggata
      361 ccgtattctt taacagac
//
LOCUS       DAYYAR010000343          368 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000343 DAYYAR010000000
VERSION     DAYYAR010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.355"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACHAT0_003041"
     CDS             complement(<1..272)
                     /locus_tag="ACHAT0_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452886.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          106 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataa
//
LOCUS       DAYYAR010000344          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000344 DAYYAR010000000
VERSION     DAYYAR010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.356"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHAT0_003042"
     CDS             complement(<1..>321)
                     /locus_tag="ACHAT0_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8452887.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYAR010000345          302 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000345 DAYYAR010000000
VERSION     DAYYAR010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.359"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>302
                     /locus_tag="ACHAT0_003043"
     CDS             <1..>302
                     /locus_tag="ACHAT0_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8452888.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          117 a           48 c           68 g           69 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 ag
//
LOCUS       DAYYAR010000346          300 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000346 DAYYAR010000000
VERSION     DAYYAR010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.360"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          106 a           33 c           48 g          113 t
ORIGIN      
        1 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacattc
       61 taatatattc atatgagcta attttatgat taaatggtaa gtttttcctt gtttgtttta
      121 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga tctgtggggt aaatcattat
      181 acaaaagtaa aattttactt attaacaggc tgtagaaata taaaaaagaa ctaaaattta
      241 actaaattat gtgctattct taaggaaagc caagtgtatg agaaagtttc tgagaatata
//
LOCUS       DAYYAR010000347          283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000347 DAYYAR010000000
VERSION     DAYYAR010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.363"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>283
                     /locus_tag="ACHAT0_003044"
     CDS             <1..>283
                     /locus_tag="ACHAT0_003044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452889.1"
                     /translation="EIWCFIKIIRSLSLQGYTPISGWIFENDILSQELPFLHEDTTVV
                     LSKNDIRINLVYNNSMEKEYFSNTLDIPVKTSSKRNKPDIRLDIILQDK"
BASE COUNT          106 a           46 c           37 g           94 t
ORIGIN      
        1 atgaaatttg gtgctttata aaaattatta gatctctatc cctgcaaggc tacactccaa
       61 tttctggatg gatttttgag aatgatattc tttcacaaga attgccgttt ttacatgaag
      121 acactaccgt agtattgagt aaaaatgata tacgtattaa tcttgtatat aataattcca
      181 tggaaaaaga atatttctcc aatacactag atatcccagt aaaaacctct tcaaaacgaa
      241 ataaacctga tattagatta gacattatcc ttcaagataa ata
//
LOCUS       DAYYAR010000348          266 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000348 DAYYAR010000000
VERSION     DAYYAR010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.366"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..>266)
                     /locus_tag="ACHAT0_003045"
     CDS             complement(34..>266)
                     /locus_tag="ACHAT0_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8452890.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaata
//
LOCUS       DAYYAR010000349          261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000349 DAYYAR010000000
VERSION     DAYYAR010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.367"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>261
                     /locus_tag="ACHAT0_003046"
     CDS             <1..>261
                     /locus_tag="ACHAT0_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452891.1"
                     /translation="PGQAATITIGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPG
                     INATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNATTTT"
BASE COUNT           87 a           54 c           56 g           64 t
ORIGIN      
        1 tcctggccaa gcagcaacaa ttaccatagg aaccgtttct agtggttcaa ccgcttctgt
       61 tacgaatgta ggaacatcat ctgcagctag atttaatttt gttttaccaa agggagataa
      121 aggtgatcct ggaataaatg ccacaactac agctgtagct acaactacag caaatgggtt
      181 gatgtcgtcc acagataaaa ctaagttgga tggaattgca gctggagcac agaaaaatcc
      241 aggtaatgct acaactacga c
//
LOCUS       DAYYAR010000350          256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000350 DAYYAR010000000
VERSION     DAYYAR010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.368"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>256)
                     /locus_tag="ACHAT0_003047"
     CDS             complement(<1..>256)
                     /locus_tag="ACHAT0_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFM8452892.1"
                     /translation="LIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIF
                     LISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTS"
BASE COUNT          100 a           42 c           35 g           79 t
ORIGIN      
        1 agaagtatat ccaaacatag ctccaacatt tggacttgta tagacaccaa ataaccgatg
       61 ttcaataaaa ccttgtcttg caagtaatcc tggcgttcct gttgggacat gataagaaat
      121 caaaaaaata aattgataga tagatattag cgcagctaaa aaaattatga taatgaaaac
      181 attattaaaa tacaccattt ttttcttttt ttcctcttca ctttgtgaaa agtctgcagg
      241 aaaaattact atcaaa
//
LOCUS       DAYYAR010000351          253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000351 DAYYAR010000000
VERSION     DAYYAR010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.369"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACHAT0_003048"
     CDS             <1..>253
                     /locus_tag="ACHAT0_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8452893.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAYYAR010000352          252 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000352 DAYYAR010000000
VERSION     DAYYAR010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.370"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..>252)
                     /locus_tag="ACHAT0_003049"
     CDS             complement(32..>252)
                     /locus_tag="ACHAT0_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8452894.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DAYYAR010000353          248 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000353 DAYYAR010000000
VERSION     DAYYAR010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.371"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..>248)
                     /locus_tag="ACHAT0_003050"
     CDS             complement(44..>248)
                     /locus_tag="ACHAT0_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFM8452895.1"
                     /translation="KVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQI
                     RQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           63 a           62 c           49 g           74 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttg
//
LOCUS       DAYYAR010000354          241 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000354 DAYYAR010000000
VERSION     DAYYAR010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.denovo.372"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..220)
                     /locus_tag="ACHAT0_003051"
     CDS             complement(<1..220)
                     /locus_tag="ACHAT0_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8452896.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT           63 a           45 c           46 g           87 t
ORIGIN      
        1 caggaaaaat gatttcggtt ctcccacaac gtcgcttatt ttctttgtat tgttcaaagt
       61 attcaagtgc tgttccacca catttgagaa agttatagac attgtaaatt gtttggcgtc
      121 cacgtttaag ctgcttcgca acgatagcaa ccggagtttc ttgatgaaaa tatgcttcta
      181 tcattacaag ctcgtttggt gtaagatggg tataagtcat ttatgttcac tctccttgta
      241 t
//
LOCUS       DAYYAR010000355        10087 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.guided.098, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000355 DAYYAR010000000
VERSION     DAYYAR010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.guided.098"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            353..523
                     /locus_tag="ACHAT0_003052"
     CDS             353..523
                     /locus_tag="ACHAT0_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452897.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            537..1139
                     /locus_tag="ACHAT0_003053"
     CDS             537..1139
                     /locus_tag="ACHAT0_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8452898.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1155..1832
                     /locus_tag="ACHAT0_003054"
                     /pseudo
     CDS             1155..1832
                     /locus_tag="ACHAT0_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2143..2640
                     /locus_tag="ACHAT0_003055"
     CDS             2143..2640
                     /locus_tag="ACHAT0_003055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFM8452899.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2641..3057
                     /locus_tag="ACHAT0_003056"
     CDS             2641..3057
                     /locus_tag="ACHAT0_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFM8452900.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3077..4621
                     /locus_tag="ACHAT0_003057"
     CDS             3077..4621
                     /locus_tag="ACHAT0_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8452901.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4764..4988
                     /locus_tag="ACHAT0_003058"
     CDS             4764..4988
                     /locus_tag="ACHAT0_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8452902.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5003..5872
                     /locus_tag="ACHAT0_003059"
     CDS             5003..5872
                     /locus_tag="ACHAT0_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM8452903.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5853..6587
                     /locus_tag="ACHAT0_003060"
     CDS             5853..6587
                     /locus_tag="ACHAT0_003060"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8452904.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6620..7528
                     /locus_tag="ACHAT0_003061"
     CDS             6620..7528
                     /locus_tag="ACHAT0_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFM8452905.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7525..8067
                     /gene="sat4"
                     /locus_tag="ACHAT0_003062"
     CDS             7525..8067
                     /gene="sat4"
                     /locus_tag="ACHAT0_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFM8452906.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8160..8954
                     /locus_tag="ACHAT0_003063"
                     /note="aph(3')-IIIa"
     CDS             8160..8954
                     /locus_tag="ACHAT0_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8452907.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9230..9361
                     /locus_tag="ACHAT0_003064"
                     /pseudo
     CDS             9230..9361
                     /locus_tag="ACHAT0_003064"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9437..9930
                     /locus_tag="ACHAT0_003065"
                     /pseudo
     CDS             9437..9930
                     /locus_tag="ACHAT0_003065"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         3548 a         1572 c         2207 g         2760 t
ORIGIN      
        1 acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt
       61 tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta attaattatt
      121 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
      181 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag
      241 ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta
      301 taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa
      361 atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata
      421 tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa
      481 agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg
      541 ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag
      601 cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc
      661 cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat
      721 tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta
      781 agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt
      841 taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac
      901 gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat
      961 tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt
     1021 tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa
     1081 cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa
     1141 aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct
     1201 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     1261 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac
     1321 caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg
     1381 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac
     1441 ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata
     1501 tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat
     1561 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     1621 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1681 tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta
     1741 cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag
     1801 caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt
     1861 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
     1921 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
     1981 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
     2041 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
     2101 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
     2161 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
     2221 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga
     2281 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
     2341 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
     2401 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
     2461 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
     2521 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
     2581 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
     2641 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
     2701 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
     2761 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
     2821 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     2881 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     2941 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     3001 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     3061 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     3121 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     3181 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     3241 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     3301 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     3361 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     3421 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     3481 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     3541 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     3601 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     3661 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     3721 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     3781 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     3841 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     3901 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     3961 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     4021 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     4081 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     4141 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     4201 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     4261 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     4321 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     4381 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     4441 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     4501 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     4561 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     4621 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     4681 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     4741 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     4801 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     4861 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     4921 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     4981 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     5041 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     5101 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     5161 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
     5221 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
     5281 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
     5341 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
     5401 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     5461 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     5521 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
     5581 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
     5641 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
     5701 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
     5761 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     5821 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     5881 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     5941 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     6001 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     6061 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     6121 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     6181 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     6241 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     6301 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     6361 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     6421 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     6481 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     6541 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     6601 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     6661 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     6721 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     6781 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     6841 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     6901 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     6961 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     7021 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     7081 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     7141 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     7201 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     7261 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     7321 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     7381 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     7441 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     7501 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     7561 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     7621 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     7681 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     7741 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     7801 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     7861 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     7921 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     7981 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     8041 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     8101 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     8161 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     8221 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     8281 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     8341 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     8401 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     8461 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     8521 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     8581 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     8641 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     8701 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     8761 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     8821 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     8881 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     8941 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     9001 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     9061 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     9121 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     9181 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     9241 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     9301 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     9361 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     9421 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     9481 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     9541 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     9601 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     9661 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     9721 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     9781 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     9841 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     9901 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     9961 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
    10021 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
    10081 ttgtgat
//
LOCUS       DAYYAR010000356         8215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.guided.119, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000356 DAYYAR010000000
VERSION     DAYYAR010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.guided.119"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..138)
                     /locus_tag="ACHAT0_003066"
     CDS             complement(<1..138)
                     /locus_tag="ACHAT0_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8452908.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IE"
     gene            283..468
                     /locus_tag="ACHAT0_003067"
     CDS             283..468
                     /locus_tag="ACHAT0_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8452909.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            626..793
                     /locus_tag="ACHAT0_003068"
     CDS             626..793
                     /locus_tag="ACHAT0_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452910.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1411..2070
                     /gene="vanR-B"
                     /locus_tag="ACHAT0_003069"
     CDS             1411..2070
                     /gene="vanR-B"
                     /locus_tag="ACHAT0_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8452911.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2073..3416
                     /gene="vanS-B"
                     /locus_tag="ACHAT0_003070"
     CDS             2073..3416
                     /gene="vanS-B"
                     /locus_tag="ACHAT0_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8452912.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            3587..4393
                     /gene="vanY"
                     /locus_tag="ACHAT0_003071"
     CDS             3587..4393
                     /gene="vanY"
                     /locus_tag="ACHAT0_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFM8452913.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            4411..5238
                     /gene="vanW-B"
                     /locus_tag="ACHAT0_003072"
     CDS             4411..5238
                     /gene="vanW-B"
                     /locus_tag="ACHAT0_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFM8452914.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            5235..6206
                     /locus_tag="ACHAT0_003073"
     CDS             5235..6206
                     /locus_tag="ACHAT0_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8452915.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            6199..7227
                     /gene="vanB"
                     /locus_tag="ACHAT0_003074"
     CDS             6199..7227
                     /gene="vanB"
                     /locus_tag="ACHAT0_003074"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8452916.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            7233..7841
                     /locus_tag="ACHAT0_003075"
     CDS             7233..7841
                     /locus_tag="ACHAT0_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8452917.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(7907..8080)
                     /locus_tag="ACHAT0_003076"
     CDS             complement(7907..8080)
                     /locus_tag="ACHAT0_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452918.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         2349 a         1698 c         2122 g         2046 t
ORIGIN      
        1 ttcgatgttg gcaagatagc gccagtcaat atttattttc tctgccattg ctttgcgcga
       61 aacgcgcttt gcttgccggg ccgctttcac atccgcgccg aaagtttcaa agccgggaca
      121 atcttcaata ttcgccatat cgcacctccc acttacattg tatatttcat actctgcccg
      181 tggaatgttg gtatatgcac ctttcttaaa gtttatattt cacattttgt ttcttttatt
      241 cttcgagcaa aagaattata atgtaccctg tggaggtgta ctatggacta catgacgttg
      301 aaagaggcag gcgagaaatg gggtgtgccc ccacgatgga taaactacta ttgtgctgcg
      361 ggacgcatcc ccggcgctgt aaagatggcg acgatctggc tcattcccaa aactgcggaa
      421 aagccgattg acaggcgtac aaagaaagga aaggcggtta ctgaatagta taatgaaacc
      481 ggcgcatttt ggatatggtt ttgaagagag agaaagcatg gtattttgtc acggaaaaac
      541 tataagatga tagatggcag gctgctccag accgataaaa agtattctca tctgaaacag
      601 aaagaaaaga tttacacatg gatgtatgaa gaagcgaaac tttatcatga cagaaatgga
      661 aaatgcccga aacaaagacg aggaagctgc tgctgtatca gccgtatatg gtagaattga
      721 aaaggccggc atctggattc tttatggtga agtctccaga cattggataa ctgctggtct
      781 aatgcgcctt tgagtatggg gtgattgatt ttaataccgg ggaggtacgg ctttttgagg
      841 aaaatattct gggatttaca tttggcgatt attacatttc ctgtaactgc aaggacacgg
      901 atgatgaaga tatgaggaat atagacttgc tgattttcta ctgtcttgaa cggtattagg
      961 actgaatctg ctgcgcggag tgcggcaaga gcgaccggag ggagtacaca caggccctgc
     1021 ctgtgtgaaa agccataccg caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga
     1081 ggcttggggt gtccccaaca agcaaaacga gaacgccgca ggcgggcaag tggtggatca
     1141 gaatatccgt aaaggatatt ctgatccatt gcttgccagt attatcggga tgttttgaca
     1201 accgctgtaa tcgaaacatc ccaaacatta agaaggtgtt aattattatt atcttgattt
     1261 aattcgttta aaccaataat atagatacat acaccatgaa aagaagcgag tatcccaatg
     1321 acggatgatt aaaatccgaa aagaaatcgc tttgaaacta cagggaaact acagactgtt
     1381 atgttatctt cttaaaatgg agggattttt atgtcgatac gaattctact tgtcgaggat
     1441 gatgatcata tctgtaatac agtaagggcg tttctggctg aggcaggata tcaggtggat
     1501 gcctgcacag atggaaatga ggcatacacc aagttttacg aaaacactta tcaactggtt
     1561 attcttgata ttatgctgcc cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg
     1621 aaaaatgata ctcccattct gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg
     1681 gcgtttgatg cagaggcaga cgactatgta acaaagccat tcaagatgca gattttacta
     1741 aagcgggtgg aagccctgct gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc
     1801 aggctgacac ttctgccgga ggatttcacg gtactttgtg acggtacaga gctgcccctg
     1861 acacgaaaag aatttgaaat ccttttgctg ctggtgcaga acaaaggcag aaccttaacg
     1921 catgaaatca ttttgtcacg tatatgggga tatgactttg agggtgatgg cagcacagtc
     1981 cacactcata tcaaaaattt gcgggccaag ctgccggaaa atatcatcaa aaccattcgc
     2041 ggtgtaggat accgattgga ggaatcataa taatggaaag aaaagggatt ttcattaagg
     2101 ttttttccta tacgatcatt gccctgctac tgcttgtcgg tgtaacagca acactgtttg
     2161 cacagcaatt tgtgtcttat ttcagagtga tggaattaca gcaaacagta aaatcctatc
     2221 agccattggt ggaactgatt cagaatagcg ataggcttga tattcaagag gtggcagggc
     2281 tgtttcacta caataaccaa tcctttgagt tttatattga agataaagag ggaagcgtac
     2341 tctatgccac accaaatgcc aatacatcaa atagttttag acccgacttt ctttatgtgg
     2401 tacatagaga tgataatatt tcgattgttg ctcaaagcaa ggcaggtgtg ggattgcttt
     2461 atcaagggct gacaattcgg ggaattgtta tgattgcgat aatggttgta ttcagccttt
     2521 tatgcgcgta tatctttgcg cggcaaatga caacgccgat caaagcctta gcggacagtg
     2581 cgaataaaat ggcaaacctg aaagatgtac cgccgccgct ggagcgaaag gatgagcttg
     2641 gcgcactggc tcatgatatg cattccatgt atgtcaggct gaaagaaacc atcgcaaggc
     2701 tggaggatga aatcgcaagg gaacatgagt tggaggaaac acagcgatat ttctttgcgg
     2761 cagcttctca tgagctaaaa acgcccatcg cggctacaag cgttctgttg gagggaatgc
     2821 ttgaaaatat cggtgactac aaagatcatt ctaagtatct gcgcgaatgc atcaaaatga
     2881 tggataggca gggcaaaatc atttccgaaa tactggagct tgtcagcctg aatgatggga
     2941 gaatcgtacc catagctgaa ccgttggaca tagggcgcac ggttgccgag ttgctgcccg
     3001 attttcaaac cttggcagag gcaaacaacc agcggttcgt cacagatatt ccagccgggc
     3061 aaattgtcct gtccgatccg aggctgctcc aaaaggcact atccaatgtc atattgaatg
     3121 cagttcagaa cacgccgcag ggaggcgagg tacggatatg gagtgagcct ggtgctgaaa
     3181 aatgccgcct ttttgttttg aacatgggcg ttcacattga tgatactgcg cttccaaggc
     3241 tgttcacccc attctatcgc attgatcagg cgcaaagcag aaaaagtggg cgaagcggtt
     3301 taggacttgc catcgtacaa aaaacgctgg atgccatgag cctccagtat gcgctggaaa
     3361 acacctcgga tggcgttttg ttctggctgg atttaccgct cacatcaaca ttgtaaatat
     3421 ttaaaactta aatgattttg accgacaggt ataaccctgc cggtcttttt ttgctgctac
     3481 aggaaaacta cagactggct acagggaaag tacagatgcg cttgccataa taacaatcgt
     3541 accagccaca aatcgtagtt ttattgcaaa ggaggcattc gatcaaatgg aaaaaagcaa
     3601 ctatcattcc aatgcggatc atcacaaacg gcatatgaaa caatccgtgg aaaagcgggc
     3661 ttttctatgt gcgttcatta tttcgttcac agtctgcacg ctgtttttgg ggcggagact
     3721 ggcttccgta ttggaggcaa cacagatacc gcccatccct gcaactcata caggcagcag
     3781 cactgacgta gtggagaatt tggaggaaaa cgctcttgcc accgccaaag aacagggaga
     3841 tgaacaggaa tggagcctga ttttagtgaa caggcagaac cccatccccg cacagtacga
     3901 tgtggagctt gagcaactat caaatggtga gcggatagat attcggattt ctccctatct
     3961 tcaagatttg tttgatgccg caagaactga tggagtttac ccgattgtcg catccggata
     4021 ccgaacaaca gaaaaacagc aagaaattat ggatgaaaaa attgccgaat ataaggcgaa
     4081 aggctacacc tctgcacagg ctaaagcgga agcagaaact tgggtggccg tgccgggaac
     4141 gagcgagcat cagcttggtc ttgctgtgga tatcaatgcg gacggaattc attcaacagg
     4201 caacgaggtt tatagatggc tggatgaaaa cagctatcgt tttggtttta ttcgtcgcta
     4261 cccaccagac aagacagaga taaccggtgt gagcaacgag ccgtggcatt accgatatgt
     4321 tggcatcgaa gctgccacag agatgtacaa ccaaggggtt tgccttgagg aatatttaaa
     4381 accagaaaaa tgagaaaagg atgttatgtt atggacagaa aaagattgac acagcgtttc
     4441 ccgttcctgc ttccaatgag acgggcgcag agaaaaatgt gcttttatgc gggaatgaga
     4501 tttgacggct gtcgctatgc gcagacgata ggggaaaaat cgctttccca tttgctcttt
     4561 gaaacggatt gcgcattata caaccacaat accggatttg acatgatata ccaggaaaac
     4621 aaggtgttca acttaaagct ggcagcaaag accttaaacg gcttattgat aagaccgggg
     4681 gaaccctttt ctttctggtg gctggtacgc catgcggaca aagatacccc ctataaagac
     4741 ggccttacgg tgaccaatgg taaactcacc accatgtcgg gcggcggtat gtgccagatg
     4801 agcaatttac tattttggat gttcctgcat acgccattga caattatcca gcgcagaggt
     4861 cacgaagtaa aggagtttcc agagccaaac agtgacgaga ttaaaggggt ggatgcaacc
     4921 atttcagagg gctggattga tttaaaagtg cgaaacgata ccgactgcac ctaccaaata
     4981 tgggtgacct tagatgatga gaaaatcatc ggtcaggtgt ccgccgacaa agagccccaa
     5041 gcattataca aaattacaaa tggcagtatc cagtatgtcc gtgaaagtgg cgggatttat
     5101 gaatatgccc aggttaaacg gatgcaagtt gccttaggta ccggggaaat aatagattgc
     5161 aagctgcttt atacaaacaa atgcaaaatc tgttatcccc tcccggaaag tgtggatatt
     5221 caggaggaga accaatgaga aaaagtgtgg gcattactgt ttttggatgc gaacaggatg
     5281 aggcaaatgc tttccgcgct ttatcgccgg attttcatat tatccctacg ctgattagcg
     5341 atgcgatatc ggcagacaac gcaaaattgg ccgctggcaa tcaatgcgtt agcgtaggcc
     5401 ataagtccga ggtttccgag gcgacaattc ttgcgctgag aaaggtcggg gtaaaataca
     5461 tttctacccg cagcatcggc tgcgatcaca ttgatacgac tgccgccgag agaatgggaa
     5521 tctcggttgg cacggttgcg tattcgccgg acagcgttgc ggattatgct ttgatgctga
     5581 tgctgatggc catacggggt gcaaaaccca ccatgcacgc cgtggcgcaa caagatttca
     5641 gattggatcg tatccggggg aaagaactgg gggatatgac tgtgggagtt attggaaccg
     5701 gccatatcgg gcaagcggtc gtcaaaaggc tgcggggatt tggatgccat gtgctggcct
     5761 atgataacag ccgaaaaatg gatgcagatt atgtccagct tgatgagctt ctaaaaaaca
     5821 gcgatattgt tacgctccat gtgccgcttt gtgcggatac ccgccatctg atcggtcaga
     5881 agcaaattgg agagatgaag caaggcgcat ttttaatcaa caccgggcgc ggagcgcttg
     5941 tcgataccgg ggcgctggtg gaggcgttgg aaagcggaaa gctgggtggt gcggcactgg
     6001 atgtgttgga gggcgaggat cagtttgttt ataccgattg ctcgcagaaa gtgcttgacc
     6061 atcccttttt gtcgcagctt ttaaggatgc cgaatgtgat catcacaccc catacggcgt
     6121 actacactga gcgtgtgctg caggatacta cagaaaaaac aatcaggaat tgtctcaatt
     6181 ttgaaaggag tttacagcat gaatagaata aaagtcgcaa tcatcttcgg cggttgctcg
     6241 gaggaacatg atgtgtcggt aaaatccgca atagaaattg ctgcgaacat tgatacggaa
     6301 aaattcgatc cgcactacat cggaattaca aaaaacggtg tatggaagct atgcaagaag
     6361 ccatgtacgg aatgggaagc cgacagtctc cccgccatac tctccccgga taggaaaacg
     6421 catgggctgc ttgtcatgaa agaaagcgaa tacgaaacac ggcgtattga tgtggctttc
     6481 ccggttttgc atggcaaatg cggggaggat ggtgcgatac aggggctgtt tgtattgtct
     6541 ggtatcccct atgtgggctg tgatattcaa agctccgcag cttgcatgga caaatcactg
     6601 gcctacattc ttacaaaaaa tgcgggcatc gccgttcccg aatttcaaat gattgataaa
     6661 ggtgacaagc cggaggcggg tgcgcttacc taccctgtct ttgtgaagcc ggcacggtca
     6721 ggttcgtcct ttggcgtaac caaagtaaac ggtacggaag aacttaacgc tgcgatagaa
     6781 gcggcaggac aatatgatgg aaaaatctta attgagcaag cgatttcggg ctgtgaggtc
     6841 gggtgtgcgg tcatggggaa cgaggatgat ttgattgtcg gcgaagtgga tcaaatccgg
     6901 ctgagccacg gtaccttccg catccatcag gaaaacgagc cggaaaaagg ctcagaaaat
     6961 gcgatgatta cagttcccgc agacattccg gtcgaggaac gaaatcgggt gcaggaaacg
     7021 gcaaagaaag tatatcgggt gcttggatgc agagggcttg cccgtgttga tctttttttg
     7081 caggaggatg gcggcatcgt tctaaatgag gtcaataccc tgcccggttt tacatcgtac
     7141 agccgctacc cacgtatgat ggccgccgca ggaatcacgc ttcctgcact gattgacagc
     7201 ctgattacat tggcgttaaa gaggtgataa gtatggaaaa tggttttttg tttttagatg
     7261 aaatgttgca tggcgtccgt tgggatgcca agtacgccac atgggataac ttcacaggaa
     7321 aaccggtgga tgggtatgag gtgaatcgca tcatcggcac aaaggccgtg gcgtttgctc
     7381 tgcgcgaggc acaaatccat gcggctgcgc ttggctatgg cttgctttta tgggatggat
     7441 atcggccaag aactgcggtg gactgcttcc tgcgttgggc agcgcaaccg gaggacaatc
     7501 tcacaaaaga aaaattttac cccaatatag agcgagccga gttgattaca aagggttatg
     7561 tggcctcaca atccagccat agccgtggaa gcgcaattga tcttacgctc taccacctgg
     7621 atacagggga acttgtttca atgggaagta acttcgattt tatggacgaa cggtcgcatc
     7681 atacagcaaa agggataggg gatgcagagg cacaaaatcg aagatgcttg cgtaaaatca
     7741 tggaaagcag cggatttcag tcttatcgct ttgaatggtg gcactataag ttgattgatg
     7801 agccataccc cgatacctat tttaattttg ccgtttcata atgaaagtat tggttttgta
     7861 tcagattttg gatttgataa aatctatgct ggtcatggac aatcttttac tggaaagtgg
     7921 ttaaagcatc ttttgtaata cattgcaaat tatagtattc aatctgccca gcggcagaaa
     7981 agaaaaaaac cgctgttggg cagacctttc ccacgcccat tttgtatttt gctggcggcg
     8041 gttttgagaa atcaaggccg ccgttctttt ctggcagcaa aaggtgtggc ggcccatagg
     8101 aggatgccgc tatgctgttt tgctattgcc atgctctaat ccacttccac cactcaaaaa
     8161 aagccttgcc cctccatgaa ctgaacccca aaagttgaac tatttaatgg actgt
//
LOCUS       DAYYAR010000357         5665 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.guided.150, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000357 DAYYAR010000000
VERSION     DAYYAR010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.guided.150"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            184..648
                     /locus_tag="ACHAT0_003077"
     CDS             184..648
                     /locus_tag="ACHAT0_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8452919.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            632..1261
                     /locus_tag="ACHAT0_003078"
     CDS             632..1261
                     /locus_tag="ACHAT0_003078"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8452920.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1615..3093
                     /gene="msr(C)"
                     /locus_tag="ACHAT0_003079"
     CDS             1615..3093
                     /gene="msr(C)"
                     /locus_tag="ACHAT0_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8452921.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3140..3586
                     /locus_tag="ACHAT0_003080"
     CDS             3140..3586
                     /locus_tag="ACHAT0_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8452922.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3785..4300)
                     /locus_tag="ACHAT0_003081"
     CDS             complement(3785..4300)
                     /locus_tag="ACHAT0_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8452923.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4417..5619
                     /locus_tag="ACHAT0_003082"
     CDS             4417..5619
                     /locus_tag="ACHAT0_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8452924.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1752 a          948 c         1205 g         1760 t
ORIGIN      
        1 gttttagcgt tgtgctttct ttatcatttt ttccgttcat tctacaaaaa atttcttttc
       61 tctatacttt tctattgaga gcgttttttt tattacacag ctgattcttt gacttttttc
      121 tttttttatt ctattattga caagtcaatc gttgacgagt caattattag aaaggaggaa
      181 attatgtctt tagaagagat tagtcacctt ttatatcaaa tcaaattagc cgatcaaaaa
      241 atcgtccaga tctttgaaga aaatatcggt ataagtctta ctagatacga gatcatgatg
      301 agactgaaag aagaaaatcc attgctacaa agcagtttgc aagaaaagtt gcagattgat
      361 caaggagcag ttactcgtca tctgaaggta ttggaagcaa atggctatgt ttcgcgtcag
      421 agaaatcctc agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc ccagaagaaa
      481 atcgagcatt gtgaaatgga ccataaagat acaggttccg tgctatcccc ggctttcagc
      541 gatgtagaaa tgaaacaatt tctaacactt ttagataaat tgaataccca actttgcaat
      601 ataacaacag aaacgaaaga ggttaataat aatggaacaa aaaaatgatt tttcaacaat
      661 tatgactggt agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc cacacgaaga
      721 aatgctggaa atgatcaaag aagcgacgac tgcaccttct tctgtcaata tgcaaccatg
      781 gcgctttgtg gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt tgatccgttt
      841 caatacgaga caaaatgata cttcttctgc aatggtcatg atttttggcg atatgcaatg
      901 ttacgaatat ggcgaagaaa tctataacca agcttacgaa tcagggaaga tgccaaaaga
      961 agtcagagat caacaattag ctgcgatcat tccttattac aagagttttt caagagaaga
     1021 aatgaacgat gtagtcaaag tcgattctag cttagcggct atgcaattca tgttagttgc
     1081 gcgtgatcat ggctatgaaa caaatccaat tggtggagtc gaagcagatc agttggcaga
     1141 ggcatttggt ttggataaag accgttatgt tccagttatt attttatcaa taggaaaagc
     1201 ggtagaagaa ggttacgagt ctgttcgttt agcaccagaa aagatcacta cgttcgaata
     1261 aaaggttttc agttactatt cagtagctat tgcttttttt gaatcgtgtg tggtaaccta
     1321 ataaaaaagg aggtcggaaa aatgactgca tcgatgaaat tacgtttcga acttttgaat
     1381 aacaactaaa taaattcaaa agacatgtat aaacaacaat ttcatggatg gagtatgtcc
     1441 attttttcga acgacagtag aataggtgaa agctatgaca gatttggtga tttagtcata
     1501 gttatttcgt tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca gatttttctg
     1561 ctctttttta gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa acatatggaa
     1621 aatttagcag taaatataac aaatctacaa gtcagctttg gcaaccagct agaactatct
     1681 attgattctc ttcgtgtcta tcagcaagat cgtataggga tcataggaga aaatggtgtc
     1741 ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt ttcccgatca tgggaaaatc
     1801 caaacagaga tcaccttcaa ctacctgcct caattaacct atctcgctga agcaaaggac
     1861 ctaaatttgg aattagccag tcatttccag ttaagactgg aagaaacttc agagcggaaa
     1921 tggagcggag gggaagaacg aaagatcgag ttgatacgtc ttctttcttc ttatgaacaa
     1981 gggatgcttc tagacgagcc aacaacccat ctagatagaa aaagtattga tcgactgatt
     2041 gaagagcttc gttattatta tggtacgctg gtttttgtta gtcatgatcg ctattttcta
     2101 gatgagctag catcgaaaat ctgggaagta aaagacggag aaatccgaga gttttcgggg
     2161 aattatagtg cctatctcac tcaaaaggaa ttggagaaaa agactcagct acgagaagca
     2221 gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa gaaaaaacaa
     2281 gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag accggatcgg
     2341 ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa tgcgaaaaca
     2401 ttagagagaa gactccaaaa aataggagaa acaaccaagc cgcagcagat gaaacaaatc
     2461 cgttttccag taccaaaatc tcttgaactc cacagccgct atcccatcat gggacaaaat
     2521 gtccaattgg aaagaagtgg gagaacatta ctggtaaatg gtgattttca gttttcttta
     2581 ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta agacaacctt attagaacat
     2641 atccgcaaac aaggagaagg aatccttctc tctccgaaag taagctttca agtatatcag
     2701 caaaagggtt atcaaatgac atctgaagaa tccatcattc gttttgtcat gagacaaaca
     2761 gagttttcag aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc tcaggaaact
     2821 ttgacgaaac cgttatgtac attaagtggg ggagaagcga cccgtctgac gattgctttg
     2881 ctttttacta agccaagtaa tgtgttgctg ttagatgaac cgactaattt tattgatatg
     2941 gcaacgatcg aagctttaga gaagctaatg caaatatatc cgggaacgat tttgtttact
     3001 tctcatgatt cttactttgt cgagcgtacg gctgatgaag tttatgaaat aaaagggcag
     3061 aaaataaaaa aagtacttac gagaaatttt taaatgtgct acgatgattt tcgtgacaaa
     3121 tttaaaggag tggtacagat tgcctacaaa taaaaaagaa ggaataattt ttacaacaat
     3181 gatgtgtttt ttgatggtct tggggatgag cgcgtataat ttatggctgc atcaagatct
     3241 tcatttatca agtttgttga ttggtctagt gcctggattc gtggtggctt tcattttgga
     3301 tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt aaactaccag taaatccaga
     3361 aagcaaggtc caaatgatct taactatttc atgcttgatg gttttaggaa tggtgacatg
     3421 tatgtcttta ttcggtgtga tcatggaaca aggaatacct gatcaattat tctctgctta
     3481 tatgaatgct tggaaaatga atgtgattgc cgctttaccg ttgcagttga tcatcgttgg
     3541 cccgctttca agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga acactgttat
     3601 ttgaaaaaag gccttggcat gctttttgtc atagtctctt gaatgcagtt ctagaaaaat
     3661 agtgatccga acgataggtt ttggcctgtc gttcggatcg ctttttcttt tccaactgta
     3721 ttgctctttt attgaagata atttgctctt caatagatat ttctagaaag gttctgttta
     3781 tcccttactc aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat tggagttcct
     3841 gctgacaaag cataattctg cgctggatca caacataacg tccaaattct tgtttcttgt
     3901 ctagcgagag gtcattcaat tttttttgat ttttttgcat gattttgttc agctgttgtt
     3961 ctctagtgaa tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa gcatagattt
     4021 ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc ctctttgatc cattcctcta
     4081 caagagcacg tccttcatca gtcaagtggt agatcttttt ttgagagtgc tcgtctttgt
     4141 ccaaaacggt cacataaccc tgctggtcta acttggcaag cgttgtatag atctgactgt
     4201 gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc atacccagaa cgtggtttgg
     4261 ataaaagcac gcataataat atataggcta aagtattcat aaaaatccta cctctcgtct
     4321 tattttcttt attatatcac agtaaagttt ccacgtttta catgtgcata tttacatgta
     4381 aaataaatca tgtatgatgc taatagagag aaagggatgg aaatattgga gaatcaaata
     4441 gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct agttgtagca
     4501 gctacctgta tgatcgcttt tatgggagtg ggtctagtag acccaatctt aaaaacgatt
     4561 gctgcacagt taaacgctac gccagctgaa acgaccctgt tatttactag ttacatgtta
     4621 gtcactggtg tggtgatgct atttacaggt ttcttatcta gtcggatcgg tttgaaaaaa
     4681 acattgatga caggattgtt tatcatcgtt gtattcgctg gtcttggcgg attatctggg
     4741 aatatcggaa tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc tttatttgtc
     4801 tctactgctt tagcaacgat cgtcagtatt ttagttggta acacagaaaa agcaatcatg
     4861 atgtatgaag cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg tggggtcttg
     4921 ggcagtggtt catggcgtta tccatttttt ggggtagcat ttttgatgtt ttgcggtttt
     4981 atagccctag cggtactttt acctgaaact gagaaaccaa agagaaaagt aggcctttgg
     5041 gaaggtgtca aagcgttaag caatcataaa cttcgttcag ttggactaat cgcattgtta
     5101 tataatttcg gattctttac tttacttgcg tatgcgccat ttttattgat tggctattct
     5161 gaattagaag tcggttttgt attttttggc tggggtgtgc ttctggctat tgcttcgatt
     5221 ttcgttgcac cggctcttga gcggaagttt acgacttatc ggacaatttt agcagcattt
     5281 gtcttattca ttatttgttt gctgttatta ggagctggaa cttctatgcc agtccttgta
     5341 ccgatcagca ttgtgttagc aggatttttc caaggaatta tcaatacact gttgacgacg
     5401 attgctatgg aaataccagg tctgcaacga aacgttgctt cgtccagcta tagttttgtc
     5461 cgctttttcg gtggagcttt agcaccattt attgcaggtg tgattggtga acatattggt
     5521 gaaagctatg cgttttattt tgcaggtgcc attgttctta tcagtgtatg ttgtatcatt
     5581 tatcaccgta attattttgc tacaaatgaa gaagaataag aaatcagagg ttgtgaaaaa
     5641 gctgtcctgc atcaagtcat aagaa
//
LOCUS       DAYYAR010000358         3613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.guided.197, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000358 DAYYAR010000000
VERSION     DAYYAR010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.guided.197"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..343
                     /locus_tag="ACHAT0_003083"
                     /pseudo
     CDS             1..343
                     /locus_tag="ACHAT0_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(615..905)
                     /locus_tag="ACHAT0_003084"
     CDS             complement(615..905)
                     /locus_tag="ACHAT0_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452925.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1307..1543
                     /locus_tag="ACHAT0_003085"
     CDS             1307..1543
                     /locus_tag="ACHAT0_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8452926.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1550..3502
                     /locus_tag="ACHAT0_003086"
     CDS             1550..3502
                     /locus_tag="ACHAT0_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8452927.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT         1253 a          614 c          656 g         1090 t
ORIGIN      
        1 ataccttatt tgatgcaaaa gaaatctacg atagtcaaca aaaaaatttt cattatatcc
       61 agaaaaattt ttcttagtta aggacctatg gattgcagta atgcagaatt cgtcgaataa
      121 actgccaaag acatacaatc atattgcttt taaaattgac gaacaagata tagattcgtt
      181 tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg
      241 cgaggcttct tcaatttact tttacgacta tgataatcac ctcttcgagt tacataccgg
      301 tactctccag gaacgtttaa aatcctacaa ttccactacc tgagactagt aatgactagt
      361 ctctttttat ggcctctgat ccccccattg agcgaatact aatagggctt cgaattgatc
      421 tgttcaatct ttaagcaata tccctcaaat gagtttggaa agtttagtga ttcatcacaa
      481 atacttaaat acatgtagtt atatatagat tgaggttctt gtgagcgtat gctcgtgtgg
      541 gttcaaatcc catcgtctgc atccatcttt ccaaaacaag cggctaggac atttttgtct
      601 cagccgcttg ttttctagtt tattctttca tcttgtcgga ccttttttct tgggtataga
      661 cttttctcat attctaccac aattttgggt ttccctaaca catgatagga tctcacaaca
      721 aattgctttc tattattata ataacctgta acgactaact tcatcccttc gtctacttca
      781 gctaggaagt tcaaactatg ggaagcaatc aaacaattga ccccatttaa actgaaacgg
      841 atcaacggtc tggcactaac ttgcaaagta cggatcttat ctactgttcc atattttttc
      901 tccataacct tcacctcaat accattatac gaacatacat tccctttgta aagaagaaac
      961 tctgttttat ctttttgact ttttgtctat tacctttctt aaaatcatat tggcacttcc
     1021 tgcgaagatg aaggacatgt actaaaaaaa agaggcagca gtatagattt aagtagttca
     1081 gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt
     1141 tgatactcgt tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa
     1201 gttaaaatgt atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt
     1261 gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga
     1321 gccaaaaaat aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat
     1381 agttaagcaa tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt
     1441 attgaacatt ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa
     1501 gcggattgtt gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct
     1561 taaaagatta gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga
     1621 agaattaaaa gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag
     1681 gtataacaaa atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat
     1741 tcaatataat tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt
     1801 caaagatgta aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg
     1861 tcataaaggt atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac
     1921 agtaacccct ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa
     1981 tagcatccaa gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga
     2041 atcaacccca ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc
     2101 tcaacgctat caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga
     2161 aaccactact tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt
     2221 tggaagggtt tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta
     2281 tcataagctg gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca
     2341 accccttcaa acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta
     2401 cctaaataac gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct
     2461 taatatgcaa acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc
     2521 tgatgttgcc tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt
     2581 caaagaagac catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta
     2641 tccacaagaa ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg
     2701 gaaaattact aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac
     2761 aactgctcac tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag
     2821 tgatgatgac ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt
     2881 gagcgatgca catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt
     2941 aaaagaattc ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa
     3001 aacaaagttt ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca
     3061 attccacgaa gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc
     3121 tatcaaacat ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga
     3181 tttatctgct ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc
     3241 gaccaatagc tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac
     3301 aacagctcgt ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca
     3361 atttgcgatt acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga
     3421 taaaaaggtt ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa
     3481 agatataatt tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt
     3541 atctttactg ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata
     3601 tacatgaaaa tag
//
LOCUS       DAYYAR010000359         2481 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.guided.227, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000359 DAYYAR010000000
VERSION     DAYYAR010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.guided.227"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..118
                     /locus_tag="ACHAT0_003087"
     CDS             <1..118
                     /locus_tag="ACHAT0_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8452928.1"
                     /translation="HKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            254..346
                     /locus_tag="ACHAT0_003088"
                     /pseudo
     CDS             254..346
                     /locus_tag="ACHAT0_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            460..1095
                     /locus_tag="ACHAT0_003089"
     CDS             460..1095
                     /locus_tag="ACHAT0_003089"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8452929.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            1202..2440
                     /gene="mef(H)"
                     /locus_tag="ACHAT0_003090"
                     /gene_synonym="mefH"
     CDS             1202..2440
                     /gene="mef(H)"
                     /locus_tag="ACHAT0_003090"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8452930.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
BASE COUNT          835 a          353 c          443 g          850 t
ORIGIN      
        1 acataagtat aagacacctt atcaagtcgt gaaaatgata aacccgaact caaaagaaga
       61 tatggaactg ttacgagaag tattaaggaa taacaatttt gatgatgagg acgagtaact
      121 tttttcctct tgcgagaaga tacagcaaac aaaaagcccg atcaagagtg caaagcacca
      181 ccaatggtgg caaagctctt gacagggctt tttcttttct gttgtgggca atcaagagga
      241 agaaagggat aaaatctgat ttgtggtaaa tcaggcaaag gcaacgcagg tatcacagta
      301 tgaattgaca ccgcataagg aaagcaactg gaagattgta gaataaatga aaaaggcttg
      361 cattctgcat ttttataaaa ttgcaagtct ttatattgac aagcataaaa tactaagata
      421 tactttaatc agaccaacgg tttgtattgg aggtaagtta tggcacgaaa taaatatcca
      481 gaagaaacag taaaactcat tttagatgtt tctacaaaaa tgttttgtga aaaaggatat
      541 gataacacat cattgcaaga tattatcaat gaaacaaaac tttctaaagg tgctatttat
      601 catcatttcg attcaaagga agatatttta aaagcaattt ttcaaagaat tggaaatgaa
      661 aatgcagtaa tatttgctaa aattagagat aacgatagtt taaacggttt agaaaagtta
      721 agagaaatat ttaagacagc ggtattcaat tctaaccaaa gcgttttatt gacagtttct
      781 cctcaactat taaacaatcc tcgttttctt gctatgcaaa tagaacaaat gtatgaaatt
      841 gtagtacctg aatttgttga acccgtttta gaacaaggta tcaaggataa gtctttggag
      901 attgaaaatt cacatgaaat tgcagaagtt atcatggtat taagtaatgt atggctcaac
      961 ccattagttg aaatgacgga taaagagggt atggaaaaac gttgcaatac attcaatgcc
     1021 cttttaaatg gaataggcat agacacatta ttagacgaag aaacaatttc tgcatttata
     1081 cagttttgta aataactgta tttgttatcc ctatacgata gactgctcta taaaagagca
     1141 gtttatttaa accattatat aaaccgacgg tctgtttgat taaataaaaa ggagagattt
     1201 tatgcaagca gaaaaaaaac tttttaatcg tgattttata cttgttgtta taggacagat
     1261 aatttcttta tttggaaacg ctatcctacg atttgcatta ccgtactatt tgttaaaaga
     1321 aactggttca tcagctttgt ttggtattgc tacagcctgc tcttttttac ctatggttat
     1381 tttgtcgctg gtaggtggta ttattgctga caggacaaat aaaagaaaca ttatggtcat
     1441 actagatttt gtaacagcaa tcataatttt agtattttat ttgtcattgg gaaaaattcc
     1501 tatcatacct ttgtttattg ttgctttgat gttactttat ggtatttctg gtgcatatca
     1561 accagcggta caagcaagta ttcctctatt ggtatcatca gataaactta ccgtcggtaa
     1621 tgctataata aatcaagtaa atactttagc aaactttatc ggaccagcta taagcggagt
     1681 agtttttcaa ttttatggta tcaatcccat tttaattgtt agtattatat gttttacttt
     1741 ttcagccatt atggaaattt ttatccatat tccacaccaa aaaagacctc gtgaagtagg
     1801 tgtaattgca gttgctaaaa acgatttaaa agatagttat acctttttga aaaaggagaa
     1861 acccattttt atatccgtaa tctttttggt ttgcattttc aatttggttt taagtgctgt
     1921 tttaattgtt ggtctgccta tcttaatttc tgaagtttta aaaatgccag atacactata
     1981 tggtttttca caatcagcat tatcattagg tggtttatgt ggtggtattt taacagctct
     2041 tgttgcttca aagttaaaac taaaaaacct ttataccttg ttacttattt gttctgtttc
     2101 tgttttactt atgggaattt cgctattttt agaattgcct gcaatagttt cctactgggt
     2161 agttacagtg atgtgctttg tggctatggg tgtatctact atgtttgttg ttcaaattta
     2221 cactcttgtt cagacacaaa caccaccgca attagtagga aagataatgg ctacgctggt
     2281 agcagttgct atgtgcggtc aacctattgg acaaatggtt tacggtattt tattcgatgt
     2341 atttatacaa gaaacatggg ttgttatgtt tgtagcttct attgcttcaa tcatgatttc
     2401 aatatattca aaaagaattt ttcaaaagtt agagatataa tacaaggggt taaatagtat
     2461 gttaggctaa aaacaatata t
//
LOCUS       DAYYAR010000360         2201 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.guided.236, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000360 DAYYAR010000000
VERSION     DAYYAR010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.guided.236"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1558
                     /gene="eat(A)"
                     /locus_tag="ACHAT0_003091"
     CDS             56..1558
                     /gene="eat(A)"
                     /locus_tag="ACHAT0_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8452931.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1625..2152
                     /locus_tag="ACHAT0_003092"
     CDS             1625..2152
                     /locus_tag="ACHAT0_003092"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8452932.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT          767 a          390 c          482 g          562 t
ORIGIN      
        1 cctctttagc cagttctgaa aataggactg gtcttttttt acgaggtgat ggaaaatgtc
       61 taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc caaggcacat tattatttga
      121 acaagcaaat ctaaattttg acacacaatg gaaactagga cttatcggac gaaacggtcg
      181 aggaaagaca actttactga atattctaca aaacaaacta ccttatcaag ggcaagtaat
      241 ccatcagcaa gaatttgcct atttcccgca acagacaaaa gataaagaac gtttaaccta
      301 ttacgtgtta aatgatatta cggattttga gatatgggaa atcgaaagag agctccaatt
      361 gatgcaaaca gatcctgaaa tcttatggag agaattcagc acactatcgg ggggagagaa
      421 gacaaaagtc ctactggcac ttttatttgt ggatgacact catttcccgt taatcgatga
      481 accaacgaat catttggata tctctggtag aaaacaagta gcggcttatt tgaaaaagaa
      541 aaaacaaggc ttcatcgtgg tcagccatga ccggggattt atcgatgaag tagtggacca
      601 tgttttagca atcgaaaaaa gtcaactgga actttatcaa gggaatttct ctatctatga
      661 agaacagaaa aaacttcgtg atgaatttga aatggctcaa aatgaaaaat tgaaaaaaga
      721 agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa tggtctcgtt cccgagaagg
      781 agataaaaca aagaaacaag tcggattcat cgatactgaa tctagacgag tgaataaagg
      841 agcagtgggt gctgatgctg cacggacgat gaaacgatcc aaagcaatcg tgaatcggat
      901 ggagacccag atcagcgaga aagaaaaact attaaaagat atcgaatata tcgattcttt
      961 gacgatgaat agccaagcgt ctcaccataa gcgactttta agcgtagaag atcttcaatt
     1021 agggtatgaa aatctgttat tcgagccaat tcattttaca atcgagcctc atcagcgggt
     1081 ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt atccattatc ttctgggggc
     1141 attcaacggc aaggttatag gagaaaaata ccagccaaaa catctgagca ttagttatgc
     1201 aagccaaaat tatgaagaca atcgaggaac gttggcggaa tttgcagaga aaaaccaagt
     1261 agactaccaa gcatttttga acaacctccg aaagcttggg atggaaagag atgtttttca
     1321 taacaagatc gagcagatga gtatgggcca acggaaaaaa gtggaattgg ctaaatcttt
     1381 atcacagcca gctgaactat atacatggga tgaaccattg aattatttgg atgtcttcaa
     1441 tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct gccatgttac tagtggaaca
     1501 tgatcaaacc ttcctggata aagtatctac tgagattatt tctcttgaga gaatctaacc
     1561 cttgtaatct caagaacaac aaggtatggt taaggggaac tttgccaacg aaggagagaa
     1621 tcacatgctt tatgtaacga gacacggaga aacaacttgg aacgcacaag gattagtttg
     1681 cggacgggca gatgtgccgc tgactgaaaa aggacagatg caggcacaga aactagcaga
     1741 aaaggtagta gatttgcctg ttccaatcac gaaaatcatc cattcccctc ttcaacgagc
     1801 aagagataca gcacaagcag ttgctgatcg tctcagcttg ccgctgaccg ttgatgaacg
     1861 attggtagaa atggattttg gggattatga tggactgcca agcaaagatg agaactttca
     1921 aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg gagtcagtct tagatgtgta
     1981 tgcacggatc gttcctttat tgaaagaatg catcgaagac gaagaaaatg tctatctgtt
     2041 agtttgccat aatgccttga taagagtgat caatgcttat tttcatccga tgcctaatga
     2101 ggggttcttt acattcatgg tagataatac agaattggtt tcttatgagt aattatgttt
     2161 ttgtgttatt taaataaaat atttcaaggc actgcttttg c
//
LOCUS       DAYYAR010000361         1075 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.guided.289, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000361 DAYYAR010000000
VERSION     DAYYAR010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.guided.289"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACHAT0_003093"
     CDS             111..194
                     /locus_tag="ACHAT0_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8452933.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACHAT0_003094"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACHAT0_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8452934.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          406 a          175 c          165 g          329 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgt
//
LOCUS       DAYYAR010000362          866 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.guided.309, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000362 DAYYAR010000000
VERSION     DAYYAR010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.guided.309"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            253..801
                     /gene="aac(6')"
                     /locus_tag="ACHAT0_003095"
     CDS             253..801
                     /gene="aac(6')"
                     /locus_tag="ACHAT0_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8452935.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          309 a          127 c          182 g          248 t
ORIGIN      
        1 ttcataattg gtttttttta aagcaaaaga taatctatat catgatgaac aaacagagtt
       61 ttttcctcca attgatggct ttcctgatct ttcctatttt gaataaagtg tttctgtata
      121 attttttatt ttataagtaa gataaataat gaaagaatag agatactgat tggtagctaa
      181 agatttttga gcgagaacat ttcgtttttt caaaaggact gttagaataa gcaagaacaa
      241 gggggaagac gtatgataat cagtgaattt gaccgtaata atccagtatt gaaagatcag
      301 ctttctgatt tactgagact gacttggccg gaagaatatg gagacagctc ggcagaagaa
      361 gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag cggtagacca agatgagtta
      421 gtaggattta ttggtgcaat ccctcaatac ggtatcacag gttgggaatt gcatccatta
      481 gttgtagaaa gctcccgacg aaagaaccaa ataggtactc gattagtcaa ttacttagaa
      541 aaagaagtag cttccagagg aggaatcacg atttatttag gtacggatga tttagaccat
      601 ggaacaacgt taagtcaaac ggacctgtat gagcatacat ttgataaagt ggcttccatc
      661 cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat taggttataa aatcgtaggt
      721 gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa aacgattatt
      781 cctcgaccaa attctcaata ataaaaagag gttgtgacag aagtaatcag cttcaagaaa
      841 taagaaggaa tttacgaaaa ttactt
//
LOCUS       DAYYAR010000363          733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5058
            SAMEA115707466-rid22593543.guided.324, whole genome shotgun
            sequence.
ACCESSION   DAYYAR010000363 DAYYAR010000000
VERSION     DAYYAR010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707466
            Sequence Read Archive: ERR13197195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290096.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290096.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:24:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,038
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 38 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 16 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707466-rid22593543.guided.324"
                     /isolate="VRE5058"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            236..733
                     /gene="dfrG"
                     /locus_tag="ACHAT0_003096"
     CDS             236..733
                     /gene="dfrG"
                     /locus_tag="ACHAT0_003096"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8452936.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          285 a           74 c          141 g          233 t
ORIGIN      
        1 cttcaaaatg gactactttt ttcattttaa cttacatacc aaattacata caaaatgatt
       61 tataaaatgc aattgaaaat gataattgaa tgaaaatttg tgattttgtg gtattatgat
      121 aatataactt atagaaaaaa ttgcttattc aactaacggg caggataatg agaaaaggtt
      181 ttttgataga aataagaaat tagatggtaa attagttaaa aaggctgtgt tataaatgaa
      241 agtttctttg attgctgcga tggataagaa tagagtgatt ggcaaagaga atgacattcc
      301 ttggaggatt cccaaggact gggaatatgt taaaaatact acaaagggac atccgataat
      361 attaggtagg aagaaccttg aatcaatcgg aagagcctta cctgacagaa gaaatattat
      421 tctgacgaga gataaggggt ttacctttaa tggttgtgaa attgttcatt caatagaaga
      481 tgtttttgag ttatgtaaaa acgaagaaga aatttttatt ttcggaggag aacagattta
      541 taatttgttt ttcccttatg ttgagaaaat gtacatcaca aaaatacatc atgaattcga
      601 aggagatact ttttttccag aagtgaatta tgaggaatgg aatgaggtat ttgcccaaaa
      661 agggataaag aatgataaaa atccgtataa ctactatttt catgtatatg aaagaaaaaa
      721 cttattgagt taa
//
